LOCUS       DAJSAW010000001       156289 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJSAW010000001 DAJSAW010000000
VERSION     DAJSAW010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 156289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 156289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..156289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.001"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(12..1535)
                     /locus_tag="ONT64_000001"
     CDS             complement(12..1535)
                     /locus_tag="ONT64_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCR2301448.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="ONT64_000002"
     CDS             1787..2968
                     /locus_tag="ONT64_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR2301449.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="ONT64_000003"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="ONT64_000003"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR2301450.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="ONT64_000004"
     CDS             complement(5053..7764)
                     /locus_tag="ONT64_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2301451.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="ONT64_000005"
     CDS             complement(7774..8475)
                     /locus_tag="ONT64_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2301452.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="ONT64_000006"
     CDS             8661..9260
                     /locus_tag="ONT64_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR2301453.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="ONT64_000007"
     CDS             9341..10354
                     /locus_tag="ONT64_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR2301454.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="ONT64_000008"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="ONT64_000008"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR2301455.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="ONT64_000009"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="ONT64_000009"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR2301456.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="ONT64_000010"
     CDS             11802..13175
                     /locus_tag="ONT64_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2301457.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="ONT64_000011"
     CDS             13532..15676
                     /locus_tag="ONT64_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR2301458.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="ONT64_000012"
     CDS             complement(15728..16768)
                     /locus_tag="ONT64_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR2301459.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="ONT64_000013"
     CDS             complement(17045..18847)
                     /locus_tag="ONT64_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2301460.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="ONT64_000014"
     CDS             complement(18861..19613)
                     /locus_tag="ONT64_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2301461.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="ONT64_000015"
     CDS             complement(19622..19984)
                     /locus_tag="ONT64_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR2301462.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="ONT64_000016"
     CDS             complement(20160..21032)
                     /locus_tag="ONT64_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCR2301463.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="ONT64_000017"
     CDS             complement(21058..21570)
                     /locus_tag="ONT64_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HCR2301464.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22499)
                     /locus_tag="ONT64_000018"
     CDS             complement(21708..22499)
                     /locus_tag="ONT64_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCR2301465.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22554..23162)
                     /locus_tag="ONT64_000019"
     CDS             complement(22554..23162)
                     /locus_tag="ONT64_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCR2301466.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="ONT64_000020"
     CDS             complement(23155..23472)
                     /locus_tag="ONT64_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR2301467.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="ONT64_000021"
     CDS             complement(23628..24356)
                     /locus_tag="ONT64_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR2301468.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="ONT64_000022"
     CDS             24515..25396
                     /locus_tag="ONT64_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2301469.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="ONT64_000023"
     CDS             complement(25633..26676)
                     /locus_tag="ONT64_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2301470.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLYFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="ONT64_000024"
     CDS             complement(26679..27347)
                     /locus_tag="ONT64_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2301471.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="ONT64_000025"
     CDS             27489..29825
                     /locus_tag="ONT64_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2301472.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="ONT64_000026"
     CDS             complement(29940..30251)
                     /locus_tag="ONT64_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301473.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="ONT64_000027"
     CDS             30432..31907
                     /locus_tag="ONT64_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HCR2301474.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="ONT64_000028"
     CDS             31954..32538
                     /locus_tag="ONT64_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR2301475.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="ONT64_000029"
     CDS             32540..32959
                     /locus_tag="ONT64_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HCR2301476.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="ONT64_000030"
     CDS             complement(33240..33524)
                     /locus_tag="ONT64_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HCR2301477.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="ONT64_000031"
     CDS             complement(33876..34586)
                     /locus_tag="ONT64_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HCR2301478.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="ONT64_000032"
     CDS             complement(34583..36055)
                     /locus_tag="ONT64_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR2301479.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="ONT64_000033"
     CDS             36193..36666
                     /locus_tag="ONT64_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR2301480.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="ONT64_000034"
     CDS             complement(36819..37370)
                     /locus_tag="ONT64_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR2301481.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="ONT64_000035"
     CDS             37475..38005
                     /locus_tag="ONT64_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HCR2301482.1"
                     /translation="MKEFKELEDFIGTRFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="ONT64_000036"
     CDS             complement(38488..38931)
                     /locus_tag="ONT64_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2301483.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="ONT64_000037"
     CDS             complement(38947..40107)
                     /locus_tag="ONT64_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR2301484.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="ONT64_000038"
     CDS             40614..42314
                     /locus_tag="ONT64_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301485.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPSFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="ONT64_000039"
     CDS             complement(42416..43249)
                     /locus_tag="ONT64_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR2301486.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="ONT64_000040"
     CDS             complement(43553..45718)
                     /locus_tag="ONT64_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2301487.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="ONT64_000041"
     CDS             45978..48272
                     /locus_tag="ONT64_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCR2301488.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="ONT64_000042"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="ONT64_000042"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR2301489.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="ONT64_000043"
     CDS             48923..49930
                     /locus_tag="ONT64_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR2301490.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="ONT64_000044"
     CDS             49952..50971
                     /locus_tag="ONT64_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2301491.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="ONT64_000045"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="ONT64_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCR2301492.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /locus_tag="ONT64_000046"
     CDS             complement(52729..54150)
                     /locus_tag="ONT64_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2301493.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="ONT64_000047"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="ONT64_000047"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCR2301494.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /locus_tag="ONT64_000048"
     CDS             complement(56561..57817)
                     /locus_tag="ONT64_000048"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR2301495.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57929..58134)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58397..59698
                     /locus_tag="ONT64_000049"
     CDS             58397..59698
                     /locus_tag="ONT64_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR2301496.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="ONT64_000050"
     CDS             complement(59767..60366)
                     /locus_tag="ONT64_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2301497.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60620..61501
                     /locus_tag="ONT64_000051"
     CDS             60620..61501
                     /locus_tag="ONT64_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2301498.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61514..63124
                     /locus_tag="ONT64_000052"
     CDS             61514..63124
                     /locus_tag="ONT64_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HCR2301499.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63201..63860)
                     /locus_tag="ONT64_000053"
     CDS             complement(63201..63860)
                     /locus_tag="ONT64_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR2301500.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64143..64808)
                     /locus_tag="ONT64_000054"
     CDS             complement(64143..64808)
                     /locus_tag="ONT64_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HCR2301501.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="ONT64_000055"
     CDS             complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="ONT64_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCR2301502.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="ONT64_000056"
     CDS             complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="ONT64_000056"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR2301503.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(66573..67019)
                     /gene="dhaM"
                     /locus_tag="ONT64_000057"
     CDS             complement(66573..67019)
                     /gene="dhaM"
                     /locus_tag="ONT64_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCR2301504.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            67122..67442
                     /locus_tag="ONT64_000058"
     CDS             67122..67442
                     /locus_tag="ONT64_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR2301505.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(67554..68423)
                     /locus_tag="ONT64_000059"
     CDS             complement(67554..68423)
                     /locus_tag="ONT64_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2301506.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(68519..69822)
                     /locus_tag="ONT64_000060"
                     /pseudo
     CDS             complement(68519..69822)
                     /locus_tag="ONT64_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(69819..70328)
                     /locus_tag="ONT64_000061"
     CDS             complement(69819..70328)
                     /locus_tag="ONT64_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301507.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(70503..72638)
                     /locus_tag="ONT64_000062"
     CDS             complement(70503..72638)
                     /locus_tag="ONT64_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2301508.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(72721..74376)
                     /locus_tag="ONT64_000063"
     CDS             complement(72721..74376)
                     /locus_tag="ONT64_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR2301509.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(74378..74692)
                     /locus_tag="ONT64_000064"
     CDS             complement(74378..74692)
                     /locus_tag="ONT64_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR2301510.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            74862..75122
                     /locus_tag="ONT64_000065"
     CDS             74862..75122
                     /locus_tag="ONT64_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HCR2301511.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            75136..75450
                     /locus_tag="ONT64_000066"
     CDS             75136..75450
                     /locus_tag="ONT64_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HCR2301512.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(76180..76734)
                     /locus_tag="ONT64_000067"
     CDS             complement(76180..76734)
                     /locus_tag="ONT64_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR2301513.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIRLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(76860..78788)
                     /locus_tag="ONT64_000068"
     CDS             complement(76860..78788)
                     /locus_tag="ONT64_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCR2301514.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(79094..79810)
                     /locus_tag="ONT64_000069"
     CDS             complement(79094..79810)
                     /locus_tag="ONT64_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCR2301515.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="ONT64_000070"
     CDS             complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="ONT64_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HCR2301516.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="ONT64_000071"
     CDS             complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="ONT64_000071"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR2301517.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(83280..84740)
                     /locus_tag="ONT64_000072"
     CDS             complement(83280..84740)
                     /locus_tag="ONT64_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2301518.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(84730..86025)
                     /locus_tag="ONT64_000073"
     CDS             complement(84730..86025)
                     /locus_tag="ONT64_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR2301519.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(86357..87034)
                     /locus_tag="ONT64_000074"
     CDS             complement(86357..87034)
                     /locus_tag="ONT64_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HCR2301520.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(87064..88155)
                     /locus_tag="ONT64_000075"
     CDS             complement(87064..88155)
                     /locus_tag="ONT64_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCR2301521.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(88256..88690)
                     /locus_tag="ONT64_000076"
     CDS             complement(88256..88690)
                     /locus_tag="ONT64_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2301522.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="ONT64_000077"
     CDS             complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="ONT64_000077"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR2301523.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(90728..92623)
                     /locus_tag="ONT64_000078"
     CDS             complement(90728..92623)
                     /locus_tag="ONT64_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2301524.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(92681..94456)
                     /locus_tag="ONT64_000079"
     CDS             complement(92681..94456)
                     /locus_tag="ONT64_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR2301525.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(94506..96002)
                     /locus_tag="ONT64_000080"
     CDS             complement(94506..96002)
                     /locus_tag="ONT64_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2301526.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(96196..97818)
                     /locus_tag="ONT64_000081"
     CDS             complement(96196..97818)
                     /locus_tag="ONT64_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR2301527.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(98267..99580)
                     /locus_tag="ONT64_000082"
     CDS             complement(98267..99580)
                     /locus_tag="ONT64_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCR2301528.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(99825..100049)
                     /locus_tag="ONT64_000083"
     CDS             complement(99825..100049)
                     /locus_tag="ONT64_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301529.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(100069..100491)
                     /locus_tag="ONT64_000084"
     CDS             complement(100069..100491)
                     /locus_tag="ONT64_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HCR2301530.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(100635..101345)
                     /locus_tag="ONT64_000085"
     CDS             complement(100635..101345)
                     /locus_tag="ONT64_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301531.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(101363..101860)
                     /locus_tag="ONT64_000086"
     CDS             complement(101363..101860)
                     /locus_tag="ONT64_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2301532.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(101897..102349)
                     /locus_tag="ONT64_000087"
     CDS             complement(101897..102349)
                     /locus_tag="ONT64_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2301533.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(102484..103089)
                     /locus_tag="ONT64_000088"
     CDS             complement(102484..103089)
                     /locus_tag="ONT64_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR2301534.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(103539..104159)
                     /locus_tag="ONT64_000089"
     CDS             complement(103539..104159)
                     /locus_tag="ONT64_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2301535.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(104161..105030)
                     /locus_tag="ONT64_000090"
     CDS             complement(104161..105030)
                     /locus_tag="ONT64_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HCR2301536.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(105053..105460)
                     /locus_tag="ONT64_000091"
     CDS             complement(105053..105460)
                     /locus_tag="ONT64_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR2301537.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(105479..106768)
                     /locus_tag="ONT64_000092"
     CDS             complement(105479..106768)
                     /locus_tag="ONT64_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2301538.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(106795..107097)
                     /locus_tag="ONT64_000093"
     CDS             complement(106795..107097)
                     /locus_tag="ONT64_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2301539.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(107122..109215)
                     /locus_tag="ONT64_000094"
     CDS             complement(107122..109215)
                     /locus_tag="ONT64_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR2301540.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(109704..110468)
                     /locus_tag="ONT64_000095"
     CDS             complement(109704..110468)
                     /locus_tag="ONT64_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HCR2301541.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="ONT64_000096"
     CDS             complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="ONT64_000096"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HCR2301542.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(111473..112549)
                     /locus_tag="ONT64_000097"
     CDS             complement(111473..112549)
                     /locus_tag="ONT64_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR2301543.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(112573..113166)
                     /locus_tag="ONT64_000098"
     CDS             complement(112573..113166)
                     /locus_tag="ONT64_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HCR2301544.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="ONT64_000099"
     CDS             complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="ONT64_000099"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HCR2301545.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(114836..115135)
                     /locus_tag="ONT64_000100"
     CDS             complement(114836..115135)
                     /locus_tag="ONT64_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HCR2301546.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="ONT64_000101"
     CDS             complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="ONT64_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HCR2301547.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(116890..117801)
                     /locus_tag="ONT64_000102"
     CDS             complement(116890..117801)
                     /locus_tag="ONT64_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR2301548.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            117921..118778
                     /locus_tag="ONT64_000103"
     CDS             117921..118778
                     /locus_tag="ONT64_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2301549.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(118965..119327)
                     /locus_tag="ONT64_000104"
     CDS             complement(118965..119327)
                     /locus_tag="ONT64_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR2301550.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            119673..121112
                     /locus_tag="ONT64_000105"
     CDS             119673..121112
                     /locus_tag="ONT64_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2301551.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(121119..123671)
                     /locus_tag="ONT64_000106"
     CDS             complement(121119..123671)
                     /locus_tag="ONT64_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HCR2301552.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(123785..125116)
                     /locus_tag="ONT64_000107"
     CDS             complement(123785..125116)
                     /locus_tag="ONT64_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2301553.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(125082..126560)
                     /locus_tag="ONT64_000108"
     CDS             complement(125082..126560)
                     /locus_tag="ONT64_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2301554.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="ONT64_000109"
     CDS             complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="ONT64_000109"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCR2301555.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            129170..130597
                     /locus_tag="ONT64_000110"
     CDS             129170..130597
                     /locus_tag="ONT64_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2301556.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(130689..131054)
                     /locus_tag="ONT64_000111"
     CDS             complement(130689..131054)
                     /locus_tag="ONT64_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCR2301557.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(131051..131416)
                     /locus_tag="ONT64_000112"
     CDS             complement(131051..131416)
                     /locus_tag="ONT64_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCR2301558.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(131608..131856)
                     /locus_tag="ONT64_000113"
     CDS             complement(131608..131856)
                     /locus_tag="ONT64_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301559.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            131855..131983
                     /locus_tag="ONT64_000114"
     CDS             131855..131983
                     /locus_tag="ONT64_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HCR2301560.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            132040..133734
                     /locus_tag="ONT64_000115"
     CDS             132040..133734
                     /locus_tag="ONT64_000115"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCR2301561.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            133738..133857
                     /locus_tag="ONT64_000116"
                     /pseudo
     CDS             133738..133857
                     /locus_tag="ONT64_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            133834..134007
                     /locus_tag="ONT64_000117"
     CDS             133834..134007
                     /locus_tag="ONT64_000117"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301562.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            134385..135518
                     /locus_tag="ONT64_000118"
     CDS             134385..135518
                     /locus_tag="ONT64_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR2301563.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            135549..135869
                     /locus_tag="ONT64_000119"
     CDS             135549..135869
                     /locus_tag="ONT64_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HCR2301564.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(135898..136362)
                     /locus_tag="ONT64_000120"
     CDS             complement(135898..136362)
                     /locus_tag="ONT64_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2301565.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(136494..137747)
                     /locus_tag="ONT64_000121"
     CDS             complement(136494..137747)
                     /locus_tag="ONT64_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2301566.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(137776..138093)
                     /locus_tag="ONT64_000122"
     CDS             complement(137776..138093)
                     /locus_tag="ONT64_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR2301567.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(138119..138439)
                     /locus_tag="ONT64_000123"
     CDS             complement(138119..138439)
                     /locus_tag="ONT64_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2301568.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(138903..140318)
                     /locus_tag="ONT64_000124"
     CDS             complement(138903..140318)
                     /locus_tag="ONT64_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2301569.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(140335..141651)
                     /locus_tag="ONT64_000125"
     CDS             complement(140335..141651)
                     /locus_tag="ONT64_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2301570.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(141801..142499)
                     /locus_tag="ONT64_000126"
     CDS             complement(141801..142499)
                     /locus_tag="ONT64_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR2301571.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(142788..143744)
                     /locus_tag="ONT64_000127"
     CDS             complement(142788..143744)
                     /locus_tag="ONT64_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR2301572.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(143857..144690)
                     /gene="msrA"
                     /locus_tag="ONT64_000128"
     CDS             complement(143857..144690)
                     /gene="msrA"
                     /locus_tag="ONT64_000128"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR2301573.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(144865..146499)
                     /locus_tag="ONT64_000129"
     CDS             complement(144865..146499)
                     /locus_tag="ONT64_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301574.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(146486..148354)
                     /locus_tag="ONT64_000130"
     CDS             complement(146486..148354)
                     /locus_tag="ONT64_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301575.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVL
                     YYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIK
                     FVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNN
                     DITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPIS
                     LLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAK
                     VIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKY
                     KAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENK
                     IEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            148320..149957
                     /locus_tag="ONT64_000131"
     CDS             148320..149957
                     /locus_tag="ONT64_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301576.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(150102..150842)
                     /gene="truA"
                     /locus_tag="ONT64_000132"
     CDS             complement(150102..150842)
                     /gene="truA"
                     /locus_tag="ONT64_000132"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR2301577.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(151033..151668)
                     /locus_tag="ONT64_000133"
     CDS             complement(151033..151668)
                     /locus_tag="ONT64_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCR2301578.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(151815..153056)
                     /gene="sstT"
                     /locus_tag="ONT64_000134"
     CDS             complement(151815..153056)
                     /gene="sstT"
                     /locus_tag="ONT64_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR2301579.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            153254..154252
                     /locus_tag="ONT64_000135"
     CDS             153254..154252
                     /locus_tag="ONT64_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301580.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            154265..156283
                     /locus_tag="ONT64_000136"
     CDS             154265..156283
                     /locus_tag="ONT64_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCR2301581.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
BASE COUNT        46512 a        32228 c        26992 g        50557 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaataca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcgcttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatctttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60421 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60481 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtttt
    60541 ataaaaaggt ataaaacctt tttattaaac tatttaatct tgtttagtat atatttttag
    60601 aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt
    60661 tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg
    60721 atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat
    60781 aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc
    60841 tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg
    60901 aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga
    60961 tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg
    61021 aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt
    61081 tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga
    61141 aaaattgaaa gaacagggca aatccatcct actttcttct catattctca gtgaagtcga
    61201 gcgactagcg gataaagtcg tgatcatacg cgaaggaaca gtcgtagaag aaggaacact
    61261 cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag
    61321 tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt
    61381 tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa
    61441 aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg
    61501 aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct
    61561 tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg
    61621 ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac
    61681 cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga
    61741 gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc
    61801 tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat
    61861 tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga
    61921 aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca
    61981 agcagattcg atcactgcag aaggtgcttg gctttttggg ctatctgtag gtagtatggg
    62041 gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc
    62101 gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt
    62161 agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt
    62221 tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat
    62281 cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga
    62341 aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa
    62401 taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc
    62461 tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat
    62521 ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtcg tcttgatttg
    62581 tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg
    62641 acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat
    62701 gttactagct attctatttg ctatattggc aatcctgatt agttctggtg cactagggct
    62761 ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc
    62821 cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg
    62881 atggcttcct cgaatgggta aacttgctta tatctactta gcttactcct ttgcactcaa
    62941 ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg
    63001 ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat
    63061 cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg
    63121 ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca
    63181 tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact
    63241 tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa
    63301 gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag
    63361 tgatccttcc ctgtcgcttt agctaagtca agcagataga cagcgactcg ttcttctact
    63421 ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt
    63481 gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt
    63541 ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg
    63601 cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc
    63661 tttccgctag cagataattg atacactttc atactgccgg aggccacgat cactaattga
    63721 ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt
    63781 gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca
    63841 cataaatgat catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa
    63901 cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa
    63961 ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg
    64021 agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa
    64081 acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt
    64141 gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt
    64201 tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt
    64261 tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa
    64321 tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa
    64381 tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct
    64441 gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat
    64501 tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg
    64561 tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt
    64621 ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat
    64681 ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc
    64741 tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca
    64801 agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg
    64861 gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa
    64921 aactgcgatg agcatcaccg cagttttatt gaacttaagg ttgattgatc acatctaaaa
    64981 gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc gatatgtccg attgcacgct
    65041 ctcctagata ggaagctcgt ccttttttag ctaccaagac tttggtgtgt tcttttgcct
    65101 ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc ctctgtgact ggcagccaaa
    65161 catctaacat cgttttatcg tcagcttctg cctttcctcg gctttgaata cccgctgttc
    65221 cctgctcgat cacttcgcct aatttttctt gagaatcaat agaatcgacc tcttttgtcg
    65281 ctttcatcat gttcatgaat gcagatccgt ataacggacc agaagcccca ccgactttcg
    65341 atatcatcgc catagagaat actttgaatg tatcgctgat tgtttcagga tgatcggctt
    65401 gaaaagcttc ttcataagct gaaacccctc ttcccatgtt atttccatgg tctccatcgc
    65461 caatcggggt atctagatca ctgagatatt gtttgttctg attgattgcc tctgcaaatt
    65521 gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat attatcaccc ttttatcttg
    65581 agatttttta ccatgaaatc gtctgtacat tgctttctaa agaatctttc cattgtgtct
    65641 ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc tagtgaggtc ataaaattac
    65701 cgacttttcg gaaaactaca ttgacgcctc tttcttccaa cagagctaaa acatcattca
    65761 tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc gttgactagt acacctgctt
    65821 cttttggtaa attcgagtaa tcatctaaaa tcttagtaac aagttccttt gccagtactt
    65881 tagaaggctg catcttttct cgacgatagc ctggctcacc gtggatacca acaccatact
    65941 cgatctcatc ttctcctagt tcgaatcctg gtttccctac ttctggtaca gttgctgctc
    66001 gaagcgctag accgatcgtc ttagttgcct gtacgacctt ttctcccagt tctttcaact
    66061 cagctaggga agcaccgttt cttgctgcat cgcccacgat tttgtgtaca aggacagtac
    66121 ctgcaactcc gcgttttcca gcagtatacg tactattttc aacggcgata tcatcatcta
    66181 cgatcacact ttctacttca atatcgtcca tagcagctaa ttcctttgcc atatcgaagt
    66241 tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac tccttttcca ctatccgctt
    66301 ccttaatagc cgtctgaatc tgatctggag ttggagaagt aaacacatca ccaagcaccg
    66361 ctgcacttaa catcccttcc cctacaaacc cggcatggga tggttcatgt ccgcttcctc
    66421 caccagaaac caaaccaact tgttccgctt tctgattctt ggctacaaca cgggaagatt
    66481 ctacacgatg aacaagttct ggatagcttt ttactaatcc ttccagcatt tcttcgacaa
    66541 tattgcttgg atcattcatg atttttttca atttattccc atcctttctt tccggcattt
    66601 tgtgcttctg ttaagatttg ctgagtatca tctgttatac cggcagtgat ggctgctgca
    66661 aaagctcctt ccactaaagg cgcatccacc agatgataac gttgctgctg ctcttcttcc
    66721 aacatgtctt ttgccagttc cgagttcaat accgcgctac cgatatccgc aaaaacaaaa
    66781 taactagctg catcgcttga ttcattgact gcttctacga ttttcatcgg gtctgatccg
    66841 atctcgccat ttattccgcc gagagaaaaa atttgcacct cttcggattt ttgcatctgc
    66901 tcaatcattt ctttaatacc atctgttact ttttgactat gagaaataag caaaatactt
    66961 tttttcacaa gaatcttcct ttcttttaca tagttgctaa cgattggttt cgctttcatt
    67021 aatgaacaat tcaagtatat aataaatttc taattttggg aagatatgtg tagtgcttct
    67081 tgaagtttgt tatactttaa ttaaaagaga ggagtgatcg aatgattaca acaacaacga
    67141 acagtgtaga gggaaaagcc gtagtagctt ataaagggat cgtttttggt gaggtcatta
    67201 ctgggatcca tgcatttaaa gacttggagg cggggttgcg aaatatcttt ggcggccgct
    67261 caaaaagcta tgaaaacgaa ctaatgggtg caagagaaga agcacttgcc gaaatggccg
    67321 aacgtgcaaa agcattagga gctgatgcga tcattggcgt aaaaatggac tatgaagtat
    67381 taggcagcga caatggcatg ctgatggtca catgtagtgg aacagctgtc tccacgagat
    67441 aagtagggga catctgtttt aataactaaa gagagatggg acatgtagtg gcactcaaga
    67501 attagatttt tcagtctaac ttcgaggtac tctaccttcc catcctctct tttttatttc
    67561 tttttgcgga tgattccgta agctgctgcg actaacacac ccgcccctgc taaggaataa
    67621 ctgacagaag ctgttttttc atttgttttt ggcaaagtct gacttgttga tttggatggc
    67681 tctgtcgttt gctgaactgt ttctgtcgtg cttccagagg tcactgattt ttctcctgcc
    67741 gaatgcaaaa tatcttttat tcgttggaaa ctagctgcta agtcagcttt ttttgcttca
    67801 aaatcattag caactgtttg tgcttcgctg atctttccgt ggattgcttg actggccgct
    67861 gtgatatccg cttgagtttg acttagcttt ccagaagctg tttgagcaac ctgatttaat
    67921 tgctgattga attcttttgc ttgttggatc ttcccgtcaa gatctgtttg gacagcttcc
    67981 gcttttgctt tcgcttcggc aagctttttc tcaagttgtg cacggtcatt tcccacttta
    68041 tttttaagat cagctaaatc ttgatcgatt gctgcttggt cttctttcaa tttgtttttt
    68101 gcagcttcta atgcttcttc gagttctttt cttttatttt ccaagtcttg caaatgatcg
    68161 atttggtcag aagaggtaga actatttatt gatgtatctt ctgaagatga aatcgtttct
    68221 cctgaattat ctgtcggtac attggaagtc atttctgttt ccgaggcacc gttttcagca
    68281 ccagaagagc ttatagttgt tgaaggttct gtttgagcag gttcagaagt ttccgcaaaa
    68341 gctgtctgtc ccaataagaa aatactggaa acagcaattg gccagattaa cggcttcatt
    68401 ctttttttca tcacttttcc catccttact actttttaac taatttctta ttataagcaa
    68461 aaatgtttgg aagaaccata aacacaacag gctgtttgtc taaaaaacgg tgcatctttt
    68521 atttttttgt acgttccggg cttttccatg tcccgcttcc ataacctaaa atcgttttga
    68581 ctgctggaat gaaagtcgtc acgatcgtta tcgcgttcac tatccagaac aaaagcatat
    68641 atagtggtgc gaaaagaagg tatttcaatt ttcttcgtcg atcatctaca atcagcgaag
    68701 ctagcaactg gaaaaatcct gcgaccattt caaagcagat gaacacaaaa ctgaatgtaa
    68761 aagtaatata aatctgttcg tagtttcctt gatatacata atgcccgatt aatccagcaa
    68821 aaagaactac ggaaatacaa aagaatattg accagacaat actcaatgtt tgatcaatga
    68881 agatgatcgt tcttccaata tgtttaaaag gatggagcat cacttttttg aaattcgtca
    68941 gccagacttc tgttccacct tttgcccaac gttttctttg tcgatatagc atcttcaacg
    69001 tcacaggtac ttccatgaaa aagataatct caggagcaaa aacagataac cagtcattta
    69061 actgatgatc ccaagcaata ctgatgtctt ctgtcgctcg atcttgtctg aatccgccca
    69121 catcgatcaa ggcttctttt cgatacatcg tatttgctcc actgtacgca taaagaccac
    69181 cgaaaacagc tgactgtgtt cgtttaatga tgcccacgat acttgagaat tcaaccgttt
    69241 gagattttgc aatcaatttg gtacgatttt ggacatccat atttgcagtg accgcagcaa
    69301 tatgtcttgc accagggcga atgaaataat tcacatacct aatcagtgca tctggctccg
    69361 gtaccgtgtc tgcatcattg cttaaaatca atttcccttt tgcaaaggca agtccgatat
    69421 taaacgcgtg ggcttttcct ttattttttt cgatccgaac cacacgaaga ttagcgtatt
    69481 ttttcatcag tctagctaat atttctggtg tttggtccgt tgaaccatcg tctgtcacta
    69541 atacctcata gttgtgataa ttcaatttcg tcatcaaata ctcgattgta tcttcgatca
    69601 caatttcttc attatgggct ggcaccatga tcgtgatgaa cggttcgaca ctcaagggaa
    69661 catctaccca ttctttttgc ttatgtttgt ataaaaacac ataacaccag acaccaataa
    69721 accatacaaa tccgcctaaa atcggataag aaacgagaaa aaatacataa tagacaaaca
    69781 agtatctaag aataaattac ctgtatgggt gattgagatc atttcagttc caccccattt
    69841 tctttataca aatctgcaat gaatgtcgta tctagatttt tctcttcgga gacagaataa
    69901 aaacggaccg tttctctctc ttcttttgtt ccaaaacgtt ccgtatacac ttcttcaagt
    69961 aattcttgtc tttttttcaa tttttcatct tcatattcaa aagatttatt tacttttttg
    70021 tatagatttc gattgttcca cctagtcaat acaaatgagg taatcaggaa actgaaaaaa
    70081 atcaatacaa aaaagccgat taagaaatac agcattttct tttctgataa aaaagcgtac
    70141 aaggaaaaat ccttggctct ttctggtctc gttaatggga aaaagaccca tataaaagga
    70201 agaacaacga tcatccatcc taaaagagcc actaacgttt gccaaatctt taaccagtaa
    70261 ttaccttttg aaaaatactt atctttcacc aaaattgatg caacttcttc tcttgttact
    70321 tttttcatat cctcacctca cgtttttttg ttacgacgta ggagtttacc atacatttca
    70381 tttcttttca cataaagtgt tcctaaattt ttcctaagaa aaagaacaaa acatttctca
    70441 tcagcaaatg aaaaatattt tgttcttttt atcagttgtt ctttttattt tttatctctt
    70501 aattatgttg ctctttccaa tacgacaatg cttttcattc ctctagatat cgccgtgtac
    70561 atttgtctgc gtcttctttg ctcatctcca tcgttttcaa atggatataa gattacatga
    70621 tcaaattcca gtccttttgc tagactcaaa ggaatgattt ggcagttttc taatccatat
    70681 gaattttttt taatcgattc ttgtagcgtt tgggtttcct cttctgtttg ggtgataact
    70741 gccgcttttt ctccttttag agaaaccaaa atctcagcta gtattttcaa caattcttca
    70801 gaggatcgaa aagagcggta ttcgggttct tttccttgcg gtcggatcga tacgatgtcg
    70861 atcttctctt gatccacagc ataggttttg aaaagctctg tgattgctcc gctagaacgg
    70921 taactatttc taagatcata ccgatggatc ggcagatggt agtcgtcgaa acaacgcata
    70981 atatcagcaa aagtagtgga agaattaaag attgcctgat tctcgtcacc tactaaagta
    71041 aagtgagttt ttggaaataa ttcgatcaat agacctaatt gtgccagggt ataatcttgt
    71101 acttcatcaa tcaatagata gcgaaatgct ggtacattta ccttttcaac aagtaaatga
    71161 cgaatagcta atataatgac tgcttcatca acagtacgtg tgggttgcca agcataagct
    71221 gagccatatc gtttttcata aattctttca aacaactgat gctcatccac ccatgccatt
    71281 tcttcgattt gtctggttat ttttcgatat tttttttgta aaagtttcct tgcataagct
    71341 acaatacttt gttccgaatc atcagaaatc aatttaccga ataattccaa ctgcctatct
    71401 tctgtcaacg agagtacctg attctgcaca gcaggacttt ttgcttgttt caaaagatga
    71461 ttcttccatt cgctcatcag tgcctttttc gttgcttgaa gcctatcgat caatttagga
    71521 tgagcaggaa ctttctcata tattttttcg atcgtttttt tagaaaggac tgtttttcca
    71581 tttttagtaa gtccacggat aaaattcgga tgatctaaaa acaaaggatc tgattgtttt
    71641 agattatcga taaacttttt gctacgcaaa cgttctgtct gctccgatac attttctccc
    71701 gtgatacgtt tgaaataagc ctcttcacct tctatttcct ctgctgaaag ctgtgaaacc
    71761 agctgtatga tggttagatt cagtggattt ctttcaccta atgatggtag gacattcgaa
    71821 atatagtcga taaaccgatg attaggtgat agaatcagca agtcatcgga tgtcatcgta
    71881 tctcgatata aatagagcag atacgcaata cgctgcatga tagcagaggt tttaccactt
    71941 cctgctacgc catttaccaa gatattttga cttttcacat cccgaatgat tgtgttttgt
    72001 tccgattgga tcgtagcagt gatagccttc atttcattgg tctcatcttg gcctaagact
    72061 tctaataata cgtcatcttg aatagctaca gacgtatcaa aaaacttgac caaacgatct
    72121 ctttccaaaa gaaattgtcg tctttcttta ataaccgtat cgattttatt ttgacgaacc
    72181 gtataagaag atggaccaat catgttattg taaaaaagtt cggcaattgg tgaacgccaa
    72241 tcataaacaa gattttcatc tgcatcatct gtgaaatttg ctgtaccgat ataaaaggat
    72301 tcttttggct cattatccag aaagtctaca acgacttttc caaagtaggg acttttcaat
    72361 aaacgttcga tctttgctag ctgattttct gcgtttttct gtttgatatt catctgatcg
    72421 atttctctat ttttcatttc aatcatcgaa aaagtatcca ggttatctaa gtaactgtca
    72481 aaattcaatc gaagctcgcc agccatttct ttcaacgact ggtttccttc tatttttgcc
    72541 gtatccatcg attcccgcag ttcgtttttt ttatcaatta gcttttgata cacgttcttc
    72601 agatatgctt cttctaactg tttttgtgtg ttgaccatac acattctcct ctttttttaa
    72661 agtggtggac ccgattagtt tacctctttt aagagtcgaa tgcaagcttt ttaacgagta
    72721 tcatttgtta ggatatatca gttcttcagg tcggacagtt tgatataaag aaaatgcacc
    72781 atcaaggttt tttacgtggt agccagactg cttcaagatt ctttccgcta gatagctacg
    72841 taatccgcta tgacagctga cgatatattc ttgttggctg tctaattcat ttaatcggtc
    72901 ccgaagttca ttcaaaggga tatgtttggc ttccttgaat ttgccctttt ccaattcttc
    72961 tgctgttcgc acatctaata aaatttttcc ttttgctagt tctgtcggca attcatgcca
    73021 ttgtacggtt tcactaagcc cttctgcaat gttcattgct gcatatccta gcatgtttac
    73081 tggatctttg gctgagccaa atggcggtgc atacgtcaat tctaattccg gtaaatcgaa
    73141 gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg atccgtttat ccacaccttt
    73201 tgccccaaca ccttgtgcac cataaatctc gccagtctct ggatggaaga tcaacttcag
    73261 taaaatatct gttgcttctg gataatagct tgcatgatct tttcctgata catgaatgac
    73321 ttcaaaagac agttcgtttt ctttggctgt tctttcactt aaaccggtgg atgcggcagt
    73381 cattccaaac gttcgtataa ttgctgttcc aataccgcct tgatttcttc gagaaattcc
    73441 ggaaatcgtg tccgcaactt gtctaccttg acgattggct ggtgaagcaa gagaaataag
    73501 tgcatctttt ccagtgattt gctgttttac aacgatagca tcccccaccg cataaatatc
    73561 aggaatattc gtttgataac gttcgtctac taggataccc ccgcgtatgc ctaattcgat
    73621 tccagcggtc actgctagct ggttatcagg acgaacacca atggataaaa taactaaatc
    73681 agcagggatg gtacgcccat catccagtcg caatcgtttc ccgtggtctt caaatccagc
    73741 gacagcatga gaagtaatca cttctactcc ttgatgagac aattcttttt ctataaaagc
    73801 tgccatttct tcatccagtg ttggaagaat atgtggtgct tgttcgatca ccatgacttc
    73861 taaacctctt ctttttaggt tttctgccat ttccaatccg atgaatcctg caccaacgat
    73921 cacggcacgc tttggctgtt tttctaatgc atgcatcact ttatcgatat ctggtacatt
    73981 tctaatagaa aaaacattgt cggcttctgt tatccctgga atcgatggaa caactggttt
    74041 tgcaccagga gataaaacca atgcatcgta ctgttcaatg tgtttttgac cctcatgtac
    74101 gacttctacc gttttgtttt cgggaaagat ggctgtcact tcatgatttg gacgcacatc
    74161 taaacaaaaa cgagcggata atgattctgg cgtttggaca agaagatttt cccgttcact
    74221 gatttctccc gaaagataat aaggcaagcc gcaatttgca aaagaaacat acggtccttt
    74281 ttcaaaaaca acgatttctg catcttccat taatcggcga agccgtgtag cagcagacat
    74341 tccacctgct acacctccga caataacgat tttcatctta tttccctcct actgttcgat
    74401 caaaccattg attcatgcca ccacgtacat tcactacatc ataaccagat ttctttaatt
    74461 ctttagctgc ttgtttactc ctcatccctg attgacaaat cacatagacg gtcttttctt
    74521 tgtctccatc atatctgttt atactttgta atggaacatt tttagctcct tttatatgcc
    74581 cgccccggta ttctgcaggt gttctaacat caagtaacgc tggattttcc aaaagctttt
    74641 cctttaattc tgggactgtt atcgatttta ttgattgaaa tagcaaattc attttctgca
    74701 cctcatttac atttttataa atacctcatg gggtatatta aacaggagct ttattaatat
    74761 gtcaaccaga atgactcttc atttttttca ctacttttta tatttgcata aaataccgat
    74821 aaggggtatt atctaattat aaaaatagag gagaatgtgt tatggagatt gataataaaa
    74881 atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg tggtatccaa aaaatgatcg
    74941 aagacgaaaa agaatgtatg gatgtcatca ctcaattaag cgctgttcgt tctagtattg
    75001 atcgcgtaat gggcatgatc gttgcagata atctgaaaaa ttgctttgag aaacctgata
    75061 gtaatccaga agaacaagcg gctaaactag aacaagcgat caagatgatt attaaaaagt
    75121 aaaggaatga tttcaatgaa agtcgttttc catattgatg aattagaaaa atggtcagaa
    75181 acagggaaaa acgtaaaaaa cctgataaaa gcttctccag aaacgacgat tgtcgtatct
    75241 gtaaatggca tcgcgattac cggttatcta gactctgcca atgcagaatt cttagatttg
    75301 caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg ctaatcatat ttcagaaagt
    75361 tctttacctg aacaagtaat cgttgtacca gcaggtgtcc ttgatttagt tgaactgcaa
    75421 tcccaaggtt atgcttatat caagccttag tcagtagagg ttgcgacaga agtgatcagc
    75481 ttcaagaaat ttcggcttat ttttcgaaga agctacttct ggaaaaccgt gaatcaaagg
    75541 ttgtgaaaac gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat
    75601 atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa
    75661 gaggttgtga aatcagcggt ttgacctaag tattagagaa aaaaacggga aaatagctcc
    75721 tcatattttg ccgttttttg agctaatacc gaaggtcagc tgattgaaca ccgtttaagg
    75781 aagagattgt gaaaaagtcg cttagtttcg agtattaaga aaaattttgt gaaaatggat
    75841 ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga ataccgttta
    75901 aggaagaggt tgtaaaatca gcggtttgac ctgagtatta gagaaacaaa cggaaaaata
    75961 gctgctcata ttttgccgtt ttttgagcta ataccgaagg tcagctgatt gaacaccgtt
    76021 taccaagagg ttgtgagcct accatttaac tccgaatcac aagaaataat ttcaaaaaca
    76081 acttctcctg tttttggttt ggttggctta ttatcgagaa gtagctactg ggagtctatt
    76141 tatcaaaaaa aggaatgcga cataggtcac accccttttt tattttcttt gaaacatctt
    76201 tcggtactcg acagcataca agacaaaaga ttgtaaaaga ccgtaagtga tgacggcaaa
    76261 aatgactctc acccacagcg ttggtaattc tgggaaaagc agcaaaagga tcatgccaac
    76321 atagatgaca gcagtcgcta ctttgccata ccacttcgct ccacttaatc tgatattttt
    76381 agatagcccg attaatccag ctacaaacat aaagccttct tttatcaaga atagaccaaa
    76441 caacagccaa agccacggtc gataccatgc catagacaaa atcagaaaaa gttgtgtcaa
    76501 tttatctgca aacggatcaa gcaccttccc taagtctgtt acctgattat atctccgagc
    76561 aatgaccccg tctagataat cagacaaacc tgagacaaca atgaccgctc ctgctatata
    76621 aaatgactgt ctcagcacaa aataaagata aatcggcagc aaaaaaatac ggaatatcga
    76681 taaaagattc ggaatcgttt gtatttcttt acgccagtct gtgcttaatt tcattttatc
    76741 gccctacctc ctcagcactc ttttcttttt attttagctt acacggggaa taaaaccaat
    76801 gatttcactg tttttatttt tctttacagc cactgatatt atgagctaat ttgagataat
    76861 cacttgctgt tttcatcgtt tactcttctt tcttcctgtc gtttgcgcat ttcttttact
    76921 agtactttat gagctttttt tgatcctcgc tctaggtcac gctgccgttt acgtgtttta
    76981 taatcgataa acagactgtc taaatcgtaa cctgtcgggt acagatcttc tgctgaacgc
    77041 accagacgaa cacgctgacg aggcacgtgg attatttttt tgtctaaata tacatcaatc
    77101 gtatcttcac ctatatcttc ataaactaac gcttcttttt ggtattcatt gacaaatatc
    77161 cgatcacctt ttgcaaagag ctggcgttct tttttattct ccttcctatt tgtaactgct
    77221 gtcttaggaa gaaattcttt tttctgtgtc cgataggctt tttccgccaa gtaacgctct
    77281 gcttgatgga tcaatttcat cgacatatcc attttatgag caatccacaa agcttggctt
    77341 tctcctgttt ctccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
    77401 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
    77461 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
    77521 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
    77581 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
    77641 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
    77701 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
    77761 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
    77821 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
    77881 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
    77941 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
    78001 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
    78061 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
    78121 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
    78181 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
    78241 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
    78301 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
    78361 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
    78421 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
    78481 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
    78541 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
    78601 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
    78661 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
    78721 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
    78781 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
    78841 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
    78901 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
    78961 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
    79021 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
    79081 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
    79141 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
    79201 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
    79261 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
    79321 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
    79381 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
    79441 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
    79501 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
    79561 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
    79621 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
    79681 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
    79741 catctccaag caaaactagg atcagtgtcc caataaactc tcccatgatt tgagtcatat
    79801 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
    79861 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
    79921 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
    79981 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
    80041 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
    80101 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
    80161 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
    80221 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
    80281 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
    80341 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
    80401 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
    80461 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
    80521 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
    80581 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
    80641 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
    80701 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
    80761 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
    80821 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
    80881 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
    80941 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
    81001 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
    81061 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
    81121 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
    81181 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
    81241 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
    81301 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
    81361 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
    81421 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
    81481 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
    81541 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
    81601 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
    81661 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
    81721 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
    81781 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
    81841 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
    81901 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
    81961 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
    82021 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
    82081 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
    82141 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
    82201 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
    82261 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
    82321 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
    82381 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
    82441 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
    82501 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
    82561 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
    82621 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
    82681 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
    82741 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
    82801 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
    82861 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
    82921 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
    82981 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
    83041 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
    83101 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
    83161 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
    83221 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
    83281 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
    83341 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
    83401 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
    83461 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
    83521 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
    83581 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
    83641 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
    83701 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
    83761 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
    83821 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
    83881 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
    83941 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
    84001 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
    84061 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
    84121 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
    84181 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
    84241 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
    84301 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
    84361 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
    84421 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
    84481 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
    84541 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
    84601 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
    84661 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
    84721 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
    84781 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
    84841 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
    84901 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
    84961 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
    85021 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
    85081 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
    85141 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
    85201 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
    85261 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
    85321 gcgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
    85381 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
    85441 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
    85501 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
    85561 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
    85621 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
    85681 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
    85741 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
    85801 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
    85861 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    85921 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    85981 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    86041 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    86101 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    86161 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    86221 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    86281 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    86341 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    86401 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    86461 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    86521 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    86581 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    86641 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    86701 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    86761 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    86821 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    86881 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    86941 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    87001 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    87061 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    87121 atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    87181 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    87241 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    87301 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    87361 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    87421 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    87481 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    87541 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    87601 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    87661 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    87721 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    87781 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    87841 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    87901 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    87961 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    88021 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    88081 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    88141 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    88201 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    88261 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    88321 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    88381 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    88441 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    88501 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    88561 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    88621 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    88681 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    88741 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    88801 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    88861 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    88921 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    88981 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    89041 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    89101 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    89161 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    89221 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    89281 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    89341 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    89401 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    89461 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgtttcaa atagtcaatg
    89521 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    89581 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    89641 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    89701 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    89761 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    89821 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    89881 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    89941 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    90001 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    90061 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    90121 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    90181 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    90241 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    90301 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    90361 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    90421 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    90481 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    90541 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    90601 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    90661 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    90721 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    90781 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    90841 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    90901 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    90961 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    91021 ttggaaaaac tgttgaaact tccccgttta caggactgta gatttttccg tccgttggaa
    91081 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    91141 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    91201 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    91261 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    91321 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    91381 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    91441 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    91501 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    91561 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    91621 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    91681 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    91741 ttgcataatc tgcaattgga cctacaacta ataaagtaat tgggaccatc acgatgatac
    91801 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    91861 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    91921 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    91981 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    92041 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    92101 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    92161 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    92221 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    92281 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    92341 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    92401 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    92461 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    92521 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    92581 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtacccctta
    92641 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    92701 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    92761 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    92821 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    92881 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    92941 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    93001 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    93061 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    93121 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    93181 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    93241 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    93301 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    93361 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    93421 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    93481 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    93541 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    93601 tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag
    93661 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
    93721 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
    93781 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
    93841 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggca
    93901 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
    93961 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
    94021 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
    94081 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
    94141 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
    94201 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
    94261 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
    94321 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
    94381 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
    94441 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
    94501 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
    94561 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
    94621 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
    94681 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
    94741 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
    94801 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
    94861 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
    94921 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
    94981 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
    95041 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
    95101 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
    95161 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
    95221 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
    95281 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
    95341 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
    95401 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
    95461 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
    95521 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
    95581 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
    95641 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
    95701 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
    95761 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
    95821 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
    95881 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
    95941 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
    96001 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
    96061 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
    96121 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
    96181 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
    96241 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
    96301 tggaacagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
    96361 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
    96421 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
    96481 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
    96541 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
    96601 cgtcctagca ttgtctcttc ctttcgcatt gattgattcc aagatttcct cttttgtaaa
    96661 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
    96721 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
    96781 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
    96841 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
    96901 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
    96961 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
    97021 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
    97081 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
    97141 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
    97201 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
    97261 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
    97321 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga
    97381 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
    97441 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
    97501 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
    97561 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
    97621 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
    97681 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
    97741 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
    97801 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
    97861 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
    97921 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
    97981 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
    98041 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat atgaggaact attttttcgt
    98101 ttttttctct aatactcagg ccagaacact gatttcacaa cctctttata aacggtgttc
    98161 actcagctgt ccctcggtat tagctcaaaa aacggcaaaa tataagaaac tattttttcg
    98221 tttttttctc taatactcag gccagaacgc tgatttcaca acctctttat ttcacattca
    98281 atatccacat gaatcccaca aatacaaaga acagaccata catgattgga gatacttctt
    98341 ttcctctttt agctgctaac atggtaattg gatagaagat aaaacctaat gcaatcccat
    98401 ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct
    98461 ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat caatactcct accataatca
    98521 atgctggtgc agttacttgt gaagtaacaa ctgatagtaa tggagaaaag aacaagccta
    98581 acaagaagaa aatccctgta gtaatagctg tgatacctga acgtcctcca acggcgatac
    98641 cagctgatga ttctacaaat gcaccaacag gagaagtacc aaataaggaa cccgcaagca
    98701 tagctgttga atcagaagct aaagctttcc ctacacgagg catcttgttg tctttcatga
    98761 aacctgcttg gtttgctaat ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca
    98821 agaatgtcag cactacgacc cacatttgaa gagaattgat gtcccctaca tgttttaatg
    98881 caactaaaaa tgttggtttc aaacttggtg ctgcggcaac aatttcagaa ggcattggaa
    98941 tcaaacctgt gaccagacct aaaatcgttg tagctgctat cccgatgaag atgccacctg
    99001 gtacacgccg aacaagcaag atagcagtta cgactaatcc aaaaatcgtc aaccaagtag
    99061 aaccaacgtt gagtgatcct aatcctacta atgtagattc attcgaaacg atgatcccac
    99121 cttcgctcag accaaggaaa gcgataaata gaccgatacc gccagaaatc gcatatttca
    99181 aatctgctgg gatcgcatca atgatcattt cccgcagctt gaagattgtg atcaaaataa
    99241 aaatcaaaga agcaacgaat acgccggcta atgctgtttc ccatggaatc cccattccta
    99301 aacaaacaga ataagcaaaa aaagcattga tcccaagagc aggtgccgtt ccaattggat
    99361 aacgggctaa tacccccatt aaaacacagc cgatcgcact ggctaatgca gtcgcagtaa
    99421 acactgctcc ttcatccatt ccagaagcac ctaatacagt tggatttaca aataagatat
    99481 aagccattga gataaatgtt gtaaatcctg ccagtacctc acgttttacc gtggtgttca
    99541 atttttctat ttcgaagtag gaaagtattt tttccttcat cttccatccc cctaaatccg
    99601 aacattataa ataaagtccg ttaaaatttc gatttattaa taaaacaacg tgttttatca
    99661 tatactaaaa caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt
    99721 ccatctgatt aaatcccttt atctcaaagt tcatgaacat tcactttgga ttacaagaat
    99781 ttctttattg tgttaataaa gtttcttcat tctagtctca aaaatcaata gtgtgatttg
    99841 attttaccat ttcgtttctg ttcttctttg atgaattctt ttgtatcgac aggattcaga
    99901 ttatttagtc cgccaaaacc tgtatcgacc atttttcccc agccttttgt cttctttttt
    99961 cctgatttga gattctttgc tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa
   100021 taaccttcta atcttcccat tagatccact gtttttcctc ctttttattc aatcagacga
   100081 ctcagctttt cctataacaa aataattgcc ttcagggtca gaaaaattga aagtcaagat
   100141 gccattgtta tctgtgattt ctcctggttc ttccatgttc tcgtagatag tttcaaattc
   100201 atccgtaaaa aatactaaag aaggcggtgg tcctaatacc tctggtgaaa acttctgcac
   100261 aaatactttt gggaatacgc ctaattctaa ttcttttgag atgctgagta caatactctt
   100321 aaattcttct ggcaattcga ttgtttcaga aatttctgca ccaatttttt ctacaaagaa
   100381 tcggcaaatc atctctacgt cattgacata aagcatcaca cgtgtttttt gtatcatcat
   100441 ttgacccttc tttctatttg attttctttc ctgtctataa actgattcca ttatatcacg
   100501 aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa taaagcaata gtttctagga
   100561 ttgctttcct ccacaaactt tttttacaaa aaaaaaaaac ggcaatgaat gccgctcttc
   100621 ttatttcgat tcttttataa ttctgcttct gattcatctt tggttagtgt gtgtctaaca
   100681 ctagttacgc caaatgtttg atcgattttt tccttcaacg cttcgattgc ctgtgcgcca
   100741 cgtgccgctt gttcagcatt gatggattca atgaccagca ccgctgcttg tgtttcttcc
   100801 gtcagcattg ttctttgggc ttcacgccag ttgagacagc gacagatcgc tccttgttca
   100861 tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg
   100921 aatgattcgc caccttgggc ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct
   100981 tctccgccgc aaggtacgcc aaaagacaag gaaatactat tatagatatc tacaagcgga
   101041 ttgattggga aaaattcccg ttcttgattt gcacgtttca gtaaagcttc aatagaagaa
   101101 cgtgcacctt ttttagtctt gaatgtggta aatgcctgac gccactgagc aatgacttcg
   101161 ttttggctga atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat
   101221 aattgatgat acgtttcttc actttctttt gttacgtggt tatcgatacc atttacggat
   101281 aagatttcga tggttgcttc aggaaatact tcccagaaat tggtatccac tattactttt
   101341 ttcatctgtt cacacctctt gattagaata attgcttgat gaatagatag tctgtttggg
   101401 gatcatctcc catataaaaa acatgttcgc tgtatttgat aaatcctttt ttacgataaa
   101461 aagcgagcgc aacatcgttg tgttcccaaa caccgagcca aatggctgtt ttttgctttt
   101521 cttttgcttt ttcaatagct gtatctagta aaaatgtacc gataccttga tgtttaaatt
   101581 tttttcgtac atagattcgc tcgatttcta aaaaatgatc agccatttcc tcagattgac
   101641 tcgttcctgt atttagtttc aggtaacccg caggctcttg atcgatactc actaggaaaa
   101701 aatcggtttc tttatctttt agctcttctg taagtttttc tagtgcataa gcttgtgtca
   101761 agtaatcagc taaatgctcc ggtgaattat cttttccaaa tgtatctgta aatgtttcga
   101821 tactaatttg ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt tcgtatttat
   101881 tcacggcttc ttcctttcaa tagatccttt tattgccttt tttgacaaaa ttccaatctt
   101941 cgctaacatt cttgcatact ttatttaaaa gatattccaa ttgttttgct tcttcgtcag
   102001 aaagaccatt taatgcgact gcattggagt agttgttttc tcgaatgatg aaaggataga
   102061 tttctgctcc ttttttagtc ggatagagtt tcttgatttt tttattttct ttatcttcca
   102121 aacgttcgat catcccattg gattctaatt tcttgattgc tcgggcagtc gttgtacgat
   102181 ctactttgat catttctgct agtttttctt ggatgattcc aggattttcg caaatacgta
   102241 ctaaataaag atactgtcct cttgttaaat cgacttcttt gaattcgata ttgctaatag
   102301 aatctagcgc tcgggcaatc attccgatgt ctcgaaggat ttcagccatt tcatcccacc
   102361 tttattttgt ttgtaagact agtttacctc tttttgttgc aaatgcaact attttcaata
   102421 aaaaaatcaa gttctttttt aataactagt aaagtatatt cttttcttct tttacgaaaa
   102481 agctcaatca caatagatct ggtttttgtt ttctaaaata agtaactgtt tcttcgttgt
   102541 tcgcttttct gatagctttg gcaactcttc tatactaaaa aatcccatgt tggaagtttc
   102601 attattctca ataaacttgg cctcttcacc gtggatcgca caagcaaaaa caagtttata
   102661 atactgaaaa agctgtggaa tatctttttg tttattcgta tcaaaaactg ctcgcaactg
   102721 agtagtttct actgttaatc ctgtttcttc atagacttct ttacgcacat tttcttcagg
   102781 agacaatcct atttcagcaa atccaccagg aagtgaccat tctttcgtcc gtttatcttc
   102841 gattagtagg accttgccat cttttttgat ataggcacga acatctactt ttggtgttgg
   102901 atgtccttct ccaatcgttg tgagcgcttt tatttcttcg aaagtttctt ttcctatttc
   102961 actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa cgctctttat caaatacatc
   103021 ttttccataa tatagaccag cttgagaaag tgcgagtagt cttttgtatt gagccaactt
   103081 cttgttcatt ctcttccctt cttttttagc aaatgtatga ctatgtaatc aagtatagaa
   103141 gactggttta attacggcaa ataaatataa tgaaatctgc aataaataaa agaatgttaa
   103201 aaaactcgag aacaaaagac attcgacttc tctggggctg tgaataaatg gcgttcaaaa
   103261 gcttgcatct gcggtaataa gccccaccaa acgaaaaata tgacgagcta ttcttgtctg
   103321 gctgttctta ttaattgagg caggacagct ttttcacaac ctccccacta aacggtgttc
   103381 tcctaccaac tcctcggtca caagtcacaa tattcatcaa acatgagaag ctgttttttt
   103441 gaaattgctc cttgtgactc ggagctgaac gacaggctca cgacctctcg gtaaacagcg
   103501 ttcaaaagcc aatttttcga tattaagcca tttctttgtt acttattgtc attgatttct
   103561 ttttctaatt tatccaatag tggagtgtaa tccacttgag tatagtctgt tacatcaaat
   103621 tcatatagtg ttcctaactg gaatttgtca tattttcggt cggcgatgat ctttttgaat
   103681 gcttctttcg taatatgatt ggtccctttc gttcgatctt ccaatacgtc accatagtga
   103741 taatgatcca tgatatagct caaatgacga tctgattcac gatcacgttt gtaccgacgt
   103801 tgacataaca cttcaaagtc tgctgttagg cgaatcgtga tcacctgata tccgtatcta
   103861 tctgctaatt cttggagtcg tgctttttgc ttatcactaa aaggatactc ggacacaatc
   103921 actttctttc ctgcttccat atacaaatca agtgctttgt aatagaaagg ataaactttc
   103981 gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc cgatggattc tgcatagtat
   104041 tccttgattt catcgagcgt gatggcatac atatctggaa atcgttcccg tagtttattc
   104101 ataagatatg tttttcctgt accaggacta ccagccagaa gaatgaaata ctttttcatc
   104161 tcatttattt cctaagttaa ttggactttc caagttcata aacagtccaa tcttatgctt
   104221 gacaatttct tttactgctt catttgctgg aatcacatta tggcgaagag acttgttgat
   104281 ttctgtttca ctaaaggttt ctttagctac tttcgtccag ttgactaaaa gctccgttcc
   104341 aacattgaat ttgcggatcc cgttattgat caattcagga taatcttctt ctttcacacc
   104401 tgttcctcca tggataacta gggggacatc aactactgca tcgatttctt tcagcaaggg
   104461 aatgtttact tctgtttttg atttgaattg tccatgattc gtaccgatag caatagctaa
   104521 tgcatccacg cctgtagctt ctacaaattt cactgcgtct tccggtcttg tatatacctt
   104581 gtcattttca gcgacatgga ttccttcttc tgtaccgccg attgttccaa gttctccttc
   104641 tacggaaaca ccgtgcgcat gggcatattc tactactgct tttgtcttca ggatattttc
   104701 tttgaaaggc agatgggacc catcatacat gacagaagta tatccgctgt cgattgcttc
   104761 tttgatatca tcaaagtctc gggcgtggtc taagtgcaag actgcatcaa ccatttcttc
   104821 ttctgcaata gctgtcatta cattgactaa cacatgcatg ccgatatatt tagcggtatc
   104881 gacacttgtt tgaataatga tcggcgaacc catttctttt gctgcgccta gcatagcagg
   104941 aagcatttct aggttatgcg tattaaaagc tccaactgta aaattcaact cttctgctgt
   105001 ttttgttact tctttcaatg ttgtatacat cttttattgc caccttatct ttttatttgt
   105061 ttcctttgat cgttacttgt tttgcaattt gtcgcatctc atccattttg cccatgtagt
   105121 aatcgatccc ttgatcatca ccagcacgag cagcatctgc acgcttctgg ttatagaggc
   105181 tcatcagttt tttataccca tctccgatcg tctgtttatt ttccaaactc ttttctaaat
   105241 attggatggc ttgatcgttc acatacaatt cagaaaattt aagtccaact tgttcataaa
   105301 gtttcgctaa tttatgctta tcttgggtag tggaaatctc ttgtttcaat tgtgtaatct
   105361 ctgattgaat cttttcggtc gcttctggcg atagggcttt ttcttctttt gccagaactt
   105421 ttttcggttc tttttctttt ttcttaaaaa atccaaacat cattttccct actttcaatt
   105481 aaaatgcttt caaaactggg cttaagatcc aggcatataa gagtgctgcg gctactgtat
   105541 ctacatcggt tgctgtagca ttgatgaatc ccatcgaatt aaagatcgtt actagcaaag
   105601 cgggcaataa tgtaataaag aaaccatgag caataccgcc aatgatcgct cctcggcgtc
   105661 cgccaactgc gttgccaaat atccctgctg tccctccggc aaagaaattg gttagcatac
   105721 caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa
   105781 tcgttgtcgt aatgaaacct aaaatgacag catttgggct ataaggaaag aaaactggac
   105841 aatctaaggc agggatcgca tcaggaacta atttcatagc tatccctcga aatgccggca
   105901 cgatttcacc taacagtaga cgtacaccag ctagcaatac atacacaccc actacgaatt
   105961 ggatggcttg taaaaaggca aacatgatat agttttgatc tcctgataat tcagaagcga
   106021 atggttcgcc tgcaaataaa acagtgatga tatatagcgg gaccatcacg accatgacag
   106081 acaaataagt atcttgcaga aattcgaaat gtgtcggcaa cttgatatct tcgatcgact
   106141 ttttattttc gcctttctca ccaaaaagct tagcaacacc ggcttcaaat aagtacccga
   106201 tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt gattttacga atgatcggtt
   106261 gcgctactgc cggcatagca atggcgaaac aagctccgat aaaaccaccg accaagatca
   106321 atacgatccc tcgcagaccg gcaaaatagc caaagactgt cgtcatcgtt gccatccata
   106381 aaattgcttg accagttaaa aaaatgtact tccattttgt gaaacgagcc aaaataatat
   106441 tgaatacaaa aatggctaag aacgtcagtg caatatctct acctaaaccc aaatcattca
   106501 ttgcttgacc attgatagat tcaatagaag ggatgatccc ttgcatatga aatccttcag
   106561 taaagatttt cccgaaataa gttaaacttc caacgatgat gctcgaaccg gcactcaaca
   106621 cttgaaatcc taggagtgtc tttaacgtgc cagagatgac ttgtcctgct gatttcttct
   106681 gcagtagtag acccaacata gcaatcaatg caatggtgat cgatgcctga gtcaaaatat
   106741 tttcgatgat aaaattgata acgcccatgt ttttaccctc ttttcttttt gtatttagat
   106801 aactccctta tcttgcagaa ctggggtgat tttttcttca atttcatttt ttgaaacaat
   106861 atttttcaaa tagatcatcg ccgttttagg atcaactgcg aacttttcga actgcgaacg
   106921 aaaattttct gctgtgatga tcacatctgg tttcatggaa actgcggaag aaatatcaca
   106981 atggtccagt ttggcttcta ctcctaattt gttcaaaaca tcctcagcag acatctgtgc
   107041 tgcaaaacta cttcctaaac ctgcaccgca tacgaataaa atgttcaatt tgttcatctt
   107101 tgttctcccc cgtatcattt attatttctc aactactgaa aaaagcagtt gtttgaaatc
   107161 ttcgatattt gtgcattcaa tcaatcgatc caacttctct tcgttgttga tcaattcaat
   107221 tagttctttc atgatgttca gatgcgagta ggagtctact gctgccaaac aaaaaataat
   107281 ctttactgga tccatttcag gattaccgaa atcgattggt tgctcaatcg tagctacgct
   107341 gactcctaat ttattgaccc catcttctgg tcgggcatgg gctagtgcca tgtgtttgcc
   107401 aatcacgata tacggtccgt aatcattcac tgcacgaatc atcgcttcga catagcgctc
   107461 ttcgacaatg ttttctttaa ccaatggtct agcagctagt tcaatcgatt cctcccaagt
   107521 agtggctttt tctttgatca tgatattgct gtccgtcaaa atatctttta acataggctg
   107581 gatctccctt ttgttgattt ctaaattgtt tgtttcgaat aatttttcta acttgccata
   107641 gatgattcga tctactcggc caccactttc tttcatgaca tctattattt gagtgaataa
   107701 ctccgtcgat tcatgactgt taggctctaa ccgctgtagt tcttcatttc ttttcaaaaa
   107761 gtcttctata accaagatat tcgtctcagt taaaatcgga tcgacaacta aaagcggctt
   107821 atttcgataa ttcaattgaa aggtgctgaa aaccagattg acatccaatt tatctactaa
   107881 ttcgatttct tttgaactta aaactgcagt aatctcgatc tgaggaaact tttctttcag
   107941 attctctgct aacagatttc cagtagccat cccatggtta cagacaacga ctgatttata
   108001 aacataattc aagtctcgct tgattacact ggcgatcgtc gaaaagtgga tgacaagaaa
   108061 agctatctcg tcctccagta tcttgccacc aattacttct tcgatcgtag gaacaaaatg
   108121 agtgactgcg cggtagattg ggaaataatt tgttcggatg ttttctttca atggattcat
   108181 gacttggata tgattcttga ttcttacaat cagtgcagcc atgtgcttat agagattttc
   108241 gaataaagct tcttccttta gatggaaagg tacgtgtgtc tcgttctcta caaattggat
   108301 caattggatc gttagtaatt gagcttgtac ccactcgatt gaattggaga tatctctagc
   108361 attgaacgtc tcaatagtga aacgaagata atttctttct acttgcggga cattgtccaa
   108421 ttgaaattcc tcgatcatgt ggtcaataaa tgcagaaaag ctactgtctt ttatttctgt
   108481 atttttccaa ttaatggcag agataaattc atgtttcctg attcgctgga tccagatcaa
   108541 tgtaaaaagc aggatttgtt ttttataaat atcatcttct tgtttcaaca acttattgga
   108601 atagatctcc tgtatttttt tgatttccac caaaggaaaa taatggagaa agattttttg
   108661 agccggtgtt aattgtgcat cttgattcat aaagaagtcg gtcattccca gattctgatt
   108721 gataaaatca tagatcatcg tacggataga tcgttctgaa cctttataaa ccagtccttg
   108781 ctttccataa ctgacgatct ctatcccata actgaccagt gttgctcgta accgtctcat
   108841 atcttcatct aatgtacttt tagagatttg aaattcttct tcttttttgt ttagatggat
   108901 cgggattcgg ctgtaagcaa cggctaaaac tagatcaaaa atacgttcgc gccgattcaa
   108961 aaagccgtct ttcacatctt ttatttcagt ttctaatcga gcttgctttt ccttttccag
   109021 cttcaacaaa aaaactttgt tgcgaattgt acggatctcc ggaagctttt tactgctcaa
   109081 ccagatattc gcttcaagaa cttcctgacg gatcgtccgc tgactgacat tgaactcact
   109141 tgctagatgt tttgctttta ctggatattg ggctgtcatg attctgtgaa gtatttcaat
   109201 tgttcttgtt ttcatagaaa tgatctcctt ctgatttcaa gtatatggtt ccgcttacat
   109261 aataaaaagt agatatttct gccacttgaa gtggaaagaa agtaagcaaa gcaaacaaaa
   109321 aataagccat atgctttttc atctcctcaa cgaaaaagac atacgcctat tattacaaaa
   109381 attttgccca tctatacaaa aaagaggttg tgaaaaagct gtcctgcatc aagtaataag
   109441 aacaactaaa ctaaaatagt gcttcctatt tttcgtttgg ttgttcttat tatcgcagat
   109501 gcaagctttt aaacaccatt tagtggagag gttgtgacag agatgttcag ccttgagcga
   109561 taagaagcga ttttgaaaaa tcgcttccca tattttacaa aatcgtgcct tattgccgat
   109621 aggctatctc tggcacactg tttattcaga aaaaggagct gcgacaaggc ttttgtcaca
   109681 actccgttca taaatatttc ctgtcaatcc agcataatcg gctgaataaa atcggcttgc
   109741 catctttttt ctttctcaga aaaatcaaaa taccatatct gagaaaaata ctgtcgttgt
   109801 gtcttacttc ctgagatcaa tcgcccatct tttcgatcga ctgaataatc acagcatctg
   109861 aaatcaattc ggatggcaaa actattttcg tggatctgtc gataatttcc tacttcctga
   109921 atagttattt ttttagtatg atttcttaca ccgtctgctt tgtttttttg cagaactttc
   109981 tgatgttcgg caaacagctt gtctgtataa cagcttctgg ctttttcagg agtttcttct
   110041 tcccaagcgg attggatctt catgaatatc ttttggattt ccagaaactg tgctttcttt
   110101 tctttagccg ttcctgacat gcgggacaat atctccgctt tcagcctttt cttttgtctt
   110161 ttattcttga cgatagaaaa agcagaaacc cctcctactg acaaaacagc taatgcaaga
   110221 aatcccgttt gctcataact agaaggacct gtacctggac cataataccc ataagaatct
   110281 ctgccgtaag aacgactgcc aaaataactg cccccatatg aacgaccacc aaatgaacct
   110341 ctgcccgaac ttcgaccgct agatgtacct ccaccatgac cacccgcgac agcgaatcct
   110401 tgggtcggca gagcaaacaa acataggccc actaacaaaa tgattgctaa ataattgatt
   110461 tttttcattg tcttcctcgt tatcttttat tacaaaaaac tatttttatg atagccttgc
   110521 taaaaggaag agtcaataac cagatgcata aactgttttt ttagcaattt tgtcttattt
   110581 agtcattgag taacatcgga caaatgtatg tgtcggcagt ccataattcc tctatcttca
   110641 gcatgaacaa gcttgcagac aagagatctg cgcttcctcc aggagagatt tggttttcct
   110701 gacaaaagtg caacagtgct gtaaatggac gatttggtaa agcactttga ctagcagcat
   110761 gagcaagttt ttgcatttcc actaacgttt tctgatcact gcgatgcaat acgcatgtgt
   110821 catctagcga actcatcaaa actaacaaca tatgcatttg tttctgtaca tatgaatcat
   110881 tatctagctc cttagctgct tggattgcaa gcctgatatg cggataccct aatatcgctt
   110941 cttcatatgc accatttacg ccatattttt gcttcaccct atgaccatga gtggtttttt
   111001 tatgacgata ttttgtatca ggaaaagcag caagcttgct gacatcatca agtattttcg
   111061 ataaagaaaa aggctgttcc atactgattt tttgtgcaat gacactgatc aatagcccca
   111121 ttgcccaaat cgctccttta tgggtattga tgccatttgt agcaatcagc attgcctttt
   111181 ccgcttttcg cccaatctca gcaattcttt ctcttaactc ctgatcaata gcatgatgcc
   111241 atgctatttg agccatttct tcaaaaaaag gagttagcgt cttagcagac ttaagaaata
   111301 aagcatagtc catatcggaa tgtgcacccg catccagtgc atccactaat ccaggttttg
   111361 gacttaatgt tacttctgcg atcaatgctt cctctaccaa cgcagcgagt tgctgagtca
   111421 ctgctttttt tcgttcgtca tttatcatga cactcactcc ttcttcctac tattacttgc
   111481 tgaaaccttt tttgatagct acccaaatga tatgaagtaa ccagcttaaa gcaaatgcac
   111541 aacttgccgt cattagataa gctacaagaa tcatgactcc tgaaggtaac tgcagccaac
   111601 tatctttaaa caataataac agtacttgga aaacaaatac attggctaaa tacgtacgat
   111661 aagcataaac agacaaccat ttgaacaatg gcaattttgg cgaatcgttt acaatcattt
   111721 tggaagcgcc tatgaagaca gcaaagatga tgactaaact gtataaactt tggatcgtat
   111781 tcaagtaagg tgcattcgca aagctcaagt ctcctggatg tgcgagcaat gaacggacag
   111841 aaaagaacgc gataactaag gcaacaggta agaaaagata acgtactctg tccaaatatt
   111901 gatagacttt ttcgtgataa acaaatgcgg ctaccccata aatcatgaaa ctagcaacaa
   111961 accgatctaa taagtaccag ctgtcatgat aaggcccagt caatacttgt gtttgataga
   112021 acagatacca tgcgacatat aaaatcgttg aaccaatcaa tactggccat acatattttt
   112081 tattagcaaa tactttgtaa ccgagccaaa caaaaaacgg catgaacaat tggatctgca
   112141 gcatcataac ggtataccat aaatgaggcg ctccatcacc tgtgacatat ttcagtaaaa
   112201 agcttgcagt atccgtatac ggagtttgtt gttggactgc tggaaatagc caaagataag
   112261 ccgtcgtcca tagaagatac ggaacgacta gttcaaaaaa cttttctttc aaaaattccc
   112321 aataagacaa atgctgactt gtttttacca tattataaac aattgcaaaa ataaacattg
   112381 gtgctgtgta tttaacgatg acgtaaaagg taccaagaaa agctgtattt gttgcgctgt
   112441 ggctgtttgc caaagcaaag ctcaatatcg tttgcatcat aacagccgtt acagctgata
   112501 ctttctggaa gtcacttacg ttgattttga tttttttgtt ttcaaacatc tctttctctc
   112561 tctttttctt tattaccaaa gttctttggt taatttaggt ccttcgactg ttttcaatag
   112621 aatttttttc tctggctctc tcaaatactc ttttagtgaa aaaccgcctt gccgagtcac
   112681 tacttgggta tcgaattgct caaaaaaagc aggatgcgat tgaattacct gaattggcaa
   112741 ttcaatcggt gaatttgcgt acagcagcaa atcaaaatca ctggtttctt ttactgccgg
   112801 aatcccagtc gataattcaa atcctaagct gccgccgatt ccccatttac atcctgataa
   112861 tatgtttcta gcagcatgaa attgctgata aacaggataa tccatgattt tctcttgtct
   112921 atacgattct tgttcgatga tgtcccacgg atgaatgact tgtttgacgg catgttttga
   112981 gatttctgtt gaccatctct gattgcgggc aggtccacgt aaaccaatcg gtagtatttc
   113041 tgcgtgatcc gtcgacgcac gacgaactgt tccgaacatc ttttcagtta gaacgaccca
   113101 ttcaggcaag gtgaaccctt ctatttcatg tttatcaaaa aggacaatat catgtgcttg
   113161 aatcattttt tatgcatccc attcttcgtc cattttggca cggactttat ttgtttcgac
   113221 acgtccgaat gttttggctg tttcgtttgt gtatcgattt gtcagcatcg tgtcatttgt
   113281 attgcggaca tcttgaatgg cttcattgat tgcttcgatg actttgctga cattttgttc
   113341 tgaagcagct tggtcggtga tatcttctag gaaatgatag atcgctccta gttttgtata
   113401 attattgata tcgtaagcga tagctggaac tttttcagca gctgcttcga tttcagcaac
   113461 cgaacgtttt gtcacacgtg cagcactttc tttagacatc gcttggacgg taatttcttt
   113521 attgtttaaa gcaatcaagc ggcttgattg taatccatgt gctaaaaatg cacctgaaat
   113581 agcatttcct gcaatcaatc caataactgg atgtccagct tgacggagtt ttgcataagc
   113641 agcagcacta ttagccaaag ccacatggat accgatcaat tcttcgttat agccgtatgc
   113701 ttggcttggt acatcgatca caacgatgat tggccgtttc acttttttgt ctttatcttc
   113761 ttcgtaaacg tgatccaaaa tttgtcccat gcggaaacct tcaactaaac caacttcacc
   113821 gtttcgtaca cggtacatcg ggtttttagg atcaggtacg attgatgtca cgatggcttc
   113881 ttctatgaat ttacttgatt taccataata cacagtacta atagaagaag ttggattttt
   113941 catgccagtt agtgccgcaa accaatcgta accaacactt gctgcagctg gttcatttcc
   114001 ttctgctact tctggtaagt caattgattt tgcttgatgg ttagcaattg cttcattgta
   114061 atcgtcaatg gccattggtt ttgaaagatc taaagaatca agtaatgata aataaaatgc
   114121 tgagctttca ctgcgatgcg tgtcttttcg ttcgtccatt gctgcgatga cagcagcttt
   114181 gattgcttct acactatcag tcactaattc atcaatgatt tttgattcta cccgttgacg
   114241 agatcctaat gtattccaaa tcaagtcttt gtcactagaa tcaaattctc gaacgcctgc
   114301 ttcttgttca ataacttctg gtccattcaa accgactctc gctttttttg ttgcgattaa
   114361 ataactcatt aatgctgaag taatcgacat accgccaaat gatcctacac gtcctggaat
   114421 caaaccgatg actggtacat attgttttag agcaatgatt acatttccga tttctgaaat
   114481 agataacagt ccgtagtttg cttcttgcaa acgaacacca cctgtatcta gtacaatgac
   114541 tggaaagatc tcatttcctt ttttattttc ttctaacaca tgttcaagtg cagcaacgat
   114601 ttttgcaccg gaaacttcac cgataccgcc accttggaat tttccttcca tagcgattac
   114661 tactgcttgt ttgccattga tcgtgccacg gctgacaacg atcccgtcat cactttcagg
   114721 gacgattcct tgtgctggta aacttggcgc gatgatttga tcgaatggat caatcaattc
   114781 acgtccattg tcaagtagag cgttcactcg ttctctggcg tttaattcta caaaactatt
   114841 tttcatctga aagttcctcc attgcttgcg tcaatcgtag gtacacaact cctggtgtag
   114901 caccgaagtc attgatcacg atatctgctg aaattggata acgatcaaaa aatctcgtta
   114961 atacattgtt ccatacttcg ccgaagccat cgcttcctgt acaaattttt acagtagatt
   115021 ctgttttttc tgttggaaac atcaagattt ccaaatctcc tgaaccgacc actcctacat
   115081 ggattttctt attgatcggc tgtttcgttg gatatttata agctaattgt tccatggatc
   115141 tctccccttt ttgtaaaaca gagcatacac gttctgctta ttcgtctttc ttcttccatc
   115201 tgggtaaatg gcgacaaaag gtttttctcg ccaattttat gaccaagttc tgaatttcat
   115261 tggtggttca tacaggccat ctgaccattc gatcaaatca tcgatggttt gtgcagctaa
   115321 taatgaacgt ttcgcttcac tacgtttgat gcctagatct tctggtaatt tcacgatgcc
   115381 ggctgcacgc aagctatcta gtgattcttt cgtgctagtc attccaagag gtgtaacacc
   115441 gccaattgct gcaattgctg cttgtcgttc ttcttttgtt tttgctttgt acaaataagc
   115501 aattccttct tctgtcacga tatgtgtagt atcttcgcta taaatcatca ctggtgcatt
   115561 ggctaagcct gcttgttttt gaacagctag tgcatctaat tcttcgacaa agactggttt
   115621 tttatttgaa ccatatgttt caaccatttg aacaactaat ttttggcctt tccctaatgg
   115681 atcatcattg tcgcgaagtg attgccatgc tggtgtagaa tgacgacgac cgccaggatt
   115741 atgtcccatg ttaggcgcac cgccaaaacc agataaacgt ccttttgtta cggtagaaga
   115801 gtttccttcg taatctaatt gcaaggttga accgataaac atatcgatgg catattgtcc
   115861 tgcagcttgt gataatgttc ggttactgcg catcgtgccg tctttaccga caaaaaagac
   115921 atctgggcga gcagcaatat atttttccat accaacttcg ccaccaaaac tatggatgct
   115981 ttcaacccaa cctgattcga tggctggaat catcgttgga tgtgggttca atacccagtt
   116041 cttcgcgatt ttccctttta accctagtga ttcaccataa gttggcagta acaattcgat
   116101 agctgctgta ttgaatccaa taccgtgatt caaagattgt acgccgtgtt ctgcgtagat
   116161 tcctttgatt gccatcattg ccattagaat ttgcaattct gtgatgtttt gaggatctcg
   116221 tgtgaataaa gcttctaatt gataaggttt atctgctttc acgacaacgt ctacccagtc
   116281 accagggata tctacacgtg gtaaagaatc gacaacttcg ttgacttgaa cgatcacgat
   116341 accatctttg aaagctgcag cttcgatgat tgttggtgtt tcttccgtat tgaaacctgt
   116401 gtacaagttg ccgtctttat ctgctttatc tgcagctact aaaacaacat ttggaatcaa
   116461 atcgatgaat aagcgtccat aaagttccaa ataagtatgg atatcaccta atttcattgt
   116521 gccgtcttca atcatttggg cgacacgcaa gctttgtgca ccagcataag agaaatcgat
   116581 cttgcttgcg attccttttt caaaaatatt caaatgttct ggtcgtgaga tactagacat
   116641 gatcaaatgc agatcattca ctttttctgc atctactttt tcaagacttt ctgaaagaaa
   116701 acttgcttgt ttttgattgt cgccttctaa tacaactttg tcgccgggag caatcagttt
   116761 ttccattact tctacgattt tttcagtttc tacatacttg ccattgagca agttgctgat
   116821 tttttccaat cgctgcgctt ttttttcgcg tttagaagcc cagttgcggt ttggtttttc
   116881 cataattagt catctccatt tttgatatag aaacgtggcg tcatcaatac catgttggtc
   116941 tagattgatc gtacggatcc ctttatctac accaaatttt gtatgaatca gctttgtcaa
   117001 agtattgccg gggggaaatt caatcgtcag atccattcct aattcttttg ccaagtccat
   117061 catttggttc cactgcacag ggaaagctag gttttccaac aaatctttac gaatctcttc
   117121 tgcgtcctga gtggctcttc catccgtatt ttttatatac aaacatctag catcagatag
   117181 atggacagac tgcatatacg gcttgaattt cttgaccgta tcattcatca agagacagtg
   117241 agatggcaca gggactttca gcagcttcgc tgtttgcgct tggtgccgtt tggcttctgc
   117301 caacgtaacg gataaacctt cgatactccc agataacgta tgttgcatcg gacaattttc
   117361 atttgacaga taaactggat ggtcaggatc aaatgttttt gctacaattt cctcagcttc
   117421 gcttctcgtc acacctacta ctacgcccat tgcataaccg cttggataag cttctttcat
   117481 taagactgcg cggtgataga ctagttccgc tgcattctca aatctcaaag atcgacaagc
   117541 aacagctgca caaaaagcac caatcgaatg tcctgctgca atatcaggct gaataccgtt
   117601 tttttccatt tcatctaaat aaaatactgc ttttgtcagt aatgctagct ggataaaaac
   117661 cgtatcttga tagttttctt ctttatcaac taaccgatac cccgtcacat tttctaaacg
   117721 ttccatgtac atttcaggga tatccgctaa catccctaac ttctgactgc cttgtccagg
   117781 aaataagaaa cctgtactca tgagcctcgc ctcctcagca cttacttttt ccactgctat
   117841 cttagtcaat ttctgctata ataaaaaatg ccaaaaaaag aaaaaaccat aacaaaaagg
   117901 tatggatggg atggataatt atgaaattgc ttcatctgca gtattttaaa aaagtattcg
   117961 aaacaaaaaa tgttacccaa gcagccaaag aattatttat ttctcaacct gccttgagcc
   118021 gtgcgatcaa acatttggaa aacgaattag gtgtcccttt gttttaccat agcggcagaa
   118081 acattgagac gaccgtttat gcagaagaat tttatccata tgcagtcaag acattagaca
   118141 cattagaaga aggaatcaaa ttattgtctt cggtcaatga gaagatcgtt acttccgtca
   118201 tattgtattt agaggtagcc tctgtttcta ttccgaactt ggtccgaatt tttatggaaa
   118261 agcatcctga tatccaatta acgatcatgc aacatcattt accagatgat attaccgatc
   118321 ctgtactgta catcacttcg gagatgaagc ctggcatgac caatattcct atcatgaaag
   118381 aacctgttta tgtcgccatt cctaagaaac atcctcttgc taaaaaagaa catctgcaat
   118441 tagaagatat ccaacgcacc cccatcttga tgctgtcaaa gagcaacgcc ttccgtcata
   118501 cactggatgc agcacttgaa gcgaaaagta tcgaattgac gatcagttcg acgacagatg
   118561 atcctgcgac attacgttct attgtcaagc aaggattagc tgttagcttt ttcccaaaag
   118621 tttcttggtc ttacgataaa aatgatcctt tcgtcttgcg ggaaatcaaa gatttgccct
   118681 tgacacgaac aatttattta tcttctagct tcacagaaga tcatcctttg atcaaaatga
   118741 ttgcaaaaac gatgcgggaa ttttatttag gaaaataaaa aaaaaagagc tgggacaaaa
   118801 ttcttgtcgc agctcttttt tcgaacaaac ggtgttccag aagtaatttc ttagaaaata
   118861 agccgaaatt tcccaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
   118921 gctaaacatt tctgtcacaa cctcttctct attgtcctag acattcattg cgttgattta
   118981 aaacttccat caatcaaatc tgctagttca tctagctttt ctattttacg tagatcaata
   119041 gagatccaat gttctttatt catgtgatag gctggaaaat acccctcttt tcctcgcaaa
   119101 attccgccaa gttcaggatc gaccttcaaa tcaagaatat tgatgatcct atggtcactt
   119161 gcatcatcat agattttttc ttctggaaca gacatcagga ccccaaacca ttttttgctt
   119221 ttttgatgtc tgaatacgca ataatcagga aattttttaa aaatatattc tggagacaaa
   119281 ttgtatgttt cattgatgtg atcgattagt tcttttttcg ttagcatctg cttccatctc
   119341 tttcttattg attcgcttta ttcaaagata ccacgtaaga atagaaaaat gaatacaagt
   119401 agttgctaat caaatacttc gatatttttc ttttttatca actcttaaat gttcaatccg
   119461 cctgtgttgg tcttgctacc atctactatt aaaatgttta ttattgtttg cccacctatt
   119521 ttttgatttt agtggtatca tttttcaagt tcatgatgaa attcttatca tcagtaaatg
   119581 ctccagaaaa ataaacaatg attgaaaaat cagttgaaac ttgaccaccg tttattttct
   119641 ccgtattctt agaatagaag ggagtctgat tgatgtattc actaatgaaa aaaatcatta
   119701 ccgaaaaaga tattcgacgt catgtttctt tagtagaaca attgctgaat catacaaaga
   119761 taaccgtaaa tgaattagct gaaatcatag ggacaaccga acgaacgatt ttttctgacc
   119821 ttcaatctat tcgctctcag ctgcccgaag gctgggacat catcagcgat caagcaggaa
   119881 tcagtttgca aaaccagcaa aatttattaa caaatgacct ttgggagatt tttttcaaac
   119941 aatcagtcag tgtcgaactg ctgaaaaatc tgctttttac aaaaaaagtt gctgtccctg
   120001 attttctagc cgaccatggg ctttcttatg ggacattgaa aagacatgtg actaaaatca
   120061 atcagcgttt agcaagttac gacttgcaga tcgatttgac gaaatataca gcttgtattc
   120121 ttggaaaaga gcgtgcgatt cgtacttttt atcatcgatt gctgattcct ttcactcata
   120181 acaactattt tttcgaggat tattcgatcc atgaatccca ttattttcaa tttttaagaa
   120241 acctcagtca aactgaacta gcggttgaaa cagaagagat tttcggaaca tgttggttct
   120301 ttatcaatac gatccgtata aaagcgaatt gccgattaga ttcttctatc cataccaaca
   120361 gcacactctc ctccctgtat gactctgcat tgaaaaaatt gtatttaacg gaaggtattt
   120421 atcttaaaga cactgagctt tcttttgctt ctttctgctt tctggaaagt tggaattaca
   120481 acaataacta tggacaagaa accgctcgct gcctgcatca ttctccattt ttagaggtcc
   120541 tcgaaacgtt tgtagaggaa ctagcttcag agctgtctct tgatcaacta aaaaaaacat
   120601 ctctgactga taacttggct ctcctcatat taaaatatca tgaatcgccg atattgattg
   120661 aagcattgga ccgtcaatac catcgttttt tagaaacata tgatcagcaa tatgcacact
   120721 tgtatcaaca gaaagagaat ttactgaacg atctaaaaaa agaaatagaa atcaatgaac
   120781 caaattactt tctccaactc ttgtctctac tgatccaaca aacgattttt tctatcaaac
   120841 cgaatttgtt caatgtctat tttattttcc aaggagaacc ttcatggaag gcgttcttgc
   120901 agcaggaatt aaaagattac ttaggaaaac gtgtctgctt cttacctatc gaactaaccg
   120961 atctttccac tatttctttt gaaaaaacag atatcttgat ttccaatttc cctctagatt
   121021 ctctagatat ccctattgta tacatttctt ctattccaac gaaaaatgag ctcaatcgtt
   121081 tgactgaact cactttcaaa acttttttat agtaacggct agcgactgta ttctctagct
   121141 gttttttctt ttttgcgaaa cagtttgttc atgatccctt tttctatttc tacaactaag
   121201 aatagaagaa tcacatttgc tccaatcaat aactgttgtg tcatcgttaa acttactgta
   121261 ccgatcagct gctggccgac tggtagaaaa ataaccatcc cttgcaataa agcaaggata
   121321 cctaatgaaa gaaaaagcgc tttgttttgc aaaagacctt ggttcaatga cggcttggac
   121381 aactctctgc aattgatcat gtaaacagct tgagctaaga cgatattttg cagaagaatg
   121441 gtttgctgta agctttgccc ttcaaactgc atagccaata aataggcagg aaccatgatc
   121501 agtagggaga catagatgat ccgaaacaaa tgataaccag ataggattcc ttgctttgga
   121561 tctcttggtg ggcgcttcat gatttctgga tctggcgact cgaaccctag tgcataagat
   121621 aaagtgattg tcgttaccat attgacccat aaaatctgta ctggggacaa aggtagcggg
   121681 cgattcagca taagtgccca aatcaagatc aatccttgag ctaatgcagt tggcaagaag
   121741 aaattgatgg ttttttgcaa attatccaaa atccgccggc cctcttttac ggcttttgca
   121801 atcgtatgga aattatcatc tcctaaaacc atatcagctg cctgtttgct tacttcacta
   121861 cctttgattc ccatagcaat tcctacatcg gatcttttca aagcaggtgc atcatttacg
   121921 ccgtctccgg tcattccgac gatttctcca tttttctgca aagctttaac aattcgcagt
   121981 ttatgttctg gtgtcgttcg agcaaagaca tcgactattg gcacttgctg aatcaactct
   122041 tcatcagaca taagatccaa atcgatccct tctaaaactt tggcagtatg ttttaagcca
   122101 atttgttccg caatcgcttt tgctgtgtct ttgtgatccc ctgtaatcat tttcactgag
   122161 atccctgctt gttggcattc ctttacagct ttgactgcac tttcttttgg cggatcgatc
   122221 aatccggcga tccctgcaaa agttaattgg gttaaagtct catgcgtgag ttcctgtttc
   122281 gaatctactt ttttataagc aaatcctaaa acacgctgac cttttctcgc aaattctgca
   122341 gctgtctgac tccatgcttc ttgttctgca gaagaaagag ttgatttagc aaataatact
   122401 tctggtgcac ctttgacgta aatcaccgct cctttttcct ccgcatgcat cgttgccata
   122461 tatttgtatt cagaactgaa cggaatcttt gcttctattt ttttcaaagg tagattggct
   122521 gtttctgtgt gtcgaatcag cgcaacttct gtcggattgc cacttaaatt ttctgttttc
   122581 tgttcttctt ttgtttttaa ttcctgacag tttttcataa tggacaacac gcaggcttct
   122641 tcttttgcag ctacatccac aactgtcatt tcattttttg tcaatgttcc tgtcttatct
   122701 gaacagatca ctgtcataga acctaatgtt tctaccgaag gaagtccttt gatgatcgcc
   122761 ttttcttttg ccatttcatg tactccaacg gatagaatca tggtcaaaac agctggcaat
   122821 ccttcgggta ccattgacac caccaaagca atcgtagccg aaagaaggat atgccaatcc
   122881 ataccatatc gaaatgtcgt aaaaataact aagaataggc tcaaacaaag aatgccttga
   122941 aaaatttgtt tgtttaatcg gttgattttt ttaatcagag gcgtcgtttg ttgttcgacc
   123001 gattgcaatg cttgattgat tttaccgatt tctgtcgtgt ttcctgtttc tacaacgaca
   123061 ccgactgcag aaccagattg tacaagcgtt cctgaaaaag ccatattttt ctggtcgccg
   123121 atcgattctt cttctgtcaa aacggcatga cttttttcag atgcttctga ttctcccgtc
   123181 aaaatcgatt cttccactaa taaatcatgt gcttcgatta atcggatatc tgctggaacg
   123241 acatctccgg cacgtaagac aatgacatct ccttttacca gttctttcgc agggattttt
   123301 tcttgcatcc cgtttgatag tactatcgct tcttgtccca tcagctgctt caaaccattc
   123361 aatgattctt ccgcttttct ttcttgccag taactaacga gtccattgac gatcactacg
   123421 aataaaataa tacttccttc aatatattca ctagaagcaa atttcaaaaa agaggcaatg
   123481 atcaacacga tcatcagcag atcagtaaaa tgctttgcta tttttttcca tcgttttgtc
   123541 tgctgccttg cttttatttc attatatcct tgatcagtca ggcgtttctg tcgttcttct
   123601 tgcgacaagc cttctgttga tgtttgatag tttttcaata catcttctac cgtcaatctg
   123661 taccagttca tcctatcccc tcctgtttaa ttgatactgt tattatacgc agggaagttt
   123721 tttcaggcgt atcgattatt tcaggggggt ctgaaataat tcgttttgca aaatgtatct
   123781 gggcctaact gatatttcta ttgcttattc ttttatatac aaaatttaag aatacaaacc
   123841 agataaccaa taaaaaagga ataaagcgtg tcgtgctttc gaataacaag ctgaaagcaa
   123901 ttgaagtaaa aaacaccaac aaagcaagat ctttatatct ttgtgtagtg ttccgtttct
   123961 tttctttaat aaattgaaca tgtgcacaca agatcgaaga ccacacaaat agaaaacaac
   124021 tcgttgtcat gcctgccaaa agctgaaaaa acaatcgact atcagctaca aaaaaatgta
   124081 gacaaagacc ggcaaaaaat aacattcctg taattaaaat accgtgtaaa ggtacgcctt
   124141 tcttagaaag cgcacaaaac caatggggca aacttttttc ttttgctgtt tgatataaaa
   124201 tcctgctagt actgtagact gcactatttg ctgaggacaa agaagcaacc aaaatcatta
   124261 gattgacaat tgctgctccg ccttggcaat ggatcacttg cagcatagtc actaaaggac
   124321 tttcttttaa atctaaacaa tgccatggaa caacaaataa aatcgacaaa atcgttccta
   124381 tatagaaaaa gagaatacgc caaggcagac agttgattgc ttttttcaaa ttcttttgcg
   124441 gatcacttgt ctcgccaacg gttaagccga tcatttcaat tccagcaaaa ctgaataaaa
   124501 ccataggaaa tgagactacc attccagcaa agccaaacgg tagtaattca tcgatctgtc
   124561 caaaattttc tttgattccg atagactggg tatgaaaatc tgagacactc aatcctatac
   124621 ccaataagat aaaaaggaaa atccccaaga tcttgatgat tgagaaaaaa gattccaatt
   124681 caccaaacca cttgactgca acaaggttga ctgccattaa gatcattagt gcgctacctc
   124741 cgcttatcaa agctggtaca tgtacaaacc agaaatggat atacaatcct attgctgtta
   124801 attccgcaag tccagctcca agccagcaaa gccaataagt ccaattgaca aaaaatgccc
   124861 actttttgcc taaataatag tcaacgaaat cactgataga gttaaatgta ctatttgcta
   124921 aaaataaatc acccatggcc cgcatcaaac cataaatagc caaaccgcat atcaaataaa
   124981 cgagaataac agctggccct gctgatgcaa ttgcctttgc tgaacctaaa aataatcctg
   125041 ttccaattgc gccacctaat gcgattaact ggatatttcg attagttaga gtccgtttca
   125101 ttgccggttt ttccatcaag ttcacctttt tctttttcaa gcagcatggc gacttcctgt
   125161 tcccaagaag gtttctttat tttttcaata cagagtggta ctacaaacaa aatgacactg
   125221 atggctgtga ctgcaattag attgatccat gttccagctt tcatcgtcag tccgacagaa
   125281 aatccgattg ccgcaaataa aacaataacg acagtccaaa ctccaagatt gctctttttc
   125341 tctttttttc caattctaaa tggtcgattc aactttgcat gtgtataacg caatctcaaa
   125401 actgcgatcc ccatgatgac atagacgata cagtagataa cagtagttga atttacgagc
   125461 atcaaaaatg cttgattgac gccaggaatc agcaaataaa aaagtgcaaa gattgagata
   125521 atgacagact gagtcaaaat aacggatttg gatacgtcaa attgattcgt cttccaaaaa
   125581 ttgaattttg gtggatacgc accttttcta gcactttctg tcactgtctt tgctggtcct
   125641 gaggcccaag cagataattg gactgctaca ccaatgaaga caagtaaact aaacagattc
   125701 acaataagat gtggcaaacc aagaatttgg caataaatcg aaatagattg cgcgatatta
   125761 ttcaattcca tttgtccttt tggtacgaca tttgctacaa ctaaagcatt caaaatattg
   125821 acgaaaaata taaaaattaa cgcggcaaat actccgcgaa tatacgtttt aactggattt
   125881 tccaatcgag tgatatacac agaggacatt tcgataccag taaaaataaa catgatcggc
   125941 gcaaaataaa caaatgagga tgcagtcgta gtatctggta ttaatttgtc ccatgaaaaa
   126001 ctgcctaatg ttccggttgg tacgatccca actttgatcc acgcagcaaa ccctaataag
   126061 agcatcatcg tcaaaggcac gtacaatcct aaccaaacac cgacttttcc gccgattttt
   126121 gccatatcaa atttcaaatt caaaagagtg atgatccaat aaacgactaa aatgacaact
   126181 aatgtaaatt tgctgttcaa accaagcggc acattatcga taatattacc aaacaacgga
   126241 gcgagtgctg aagcgaccat caccatccca ggaaacattt ggacccataa cagccatgag
   126301 acaacgaacc cccatttttt tcctagagca ttcgataccc aaagttctgg accacctttt
   126361 tggggaaaca ttccagcaaa ttcaccagag atcaatgaga ttggaaaagc gtacaataat
   126421 actgcaacca gcatgtaaaa aatcatcgtc cagctcgttg ctgctacatc tggaatattt
   126481 cgtacacttg ctacaagggc gcaggtcatt ccgataaagg tcatggtcgt gatttgcttt
   126541 gatgaaccag tatctttcat ctttattcct tctttcaata gcgacatatg tttttgtttt
   126601 cttttatttt tgttcaagaa cagaaatgac aaggtttcat cgattcagta caaaacccag
   126661 tatcaccgaa acatccattg aacgatctta gtgaactggg tttgatacat gaaacgaacg
   126721 atccaactta tagaacgttt tataaaaaag aatatcttgg cgaggaatat atgaaaaaca
   126781 gggggaaata caattgtttt gatgaagcaa ataattgtct gttctccata tgtctcttgt
   126841 tattctttca cacaaaaatc agttctattg gatgatttcg tttagtttct tttccattgc
   126901 tttggcgatt ttttttgtat aaaaattgaa aattcgatca tcattcaaat aaggagtcat
   126961 gatagccgca cgtagcagag tgattttctg ttcttttttc cattcttctt tcgttagacc
   127021 cattctttca acaaaacgaa tcggagaatc accgtaatct tcttgtgtaa atgtagtatg
   127081 agaagtaata aaccgttcac cataaacatc gccatccata taagaagacc gatcgaacat
   127141 tttttcattt aattcattga tagcgttcaa atctgtacag ccttgttctt tgaataccca
   127201 attgaccata ttaaaatcag gatgattcaa tggatagact tcgattgttt ttcctttaac
   127261 agtaaaagtc agatgatcga gaaattctct gaatctttga gctgcctcga ttgaagcccc
   127321 aatcaattga ccatatccgg tcacattcaa tggcaataca cgatgtgctg tccagactgc
   127381 ggcggcggtt gctccagctt ttgatccttc caaaatataa gctcctaaca tatctggagc
   127441 ctttactgat ttttcaaaca catatggcgc gaaataagaa atgatatttc tcatattttt
   127501 atgtttgatt acgattcctc cagcagcata agggacatag cccatcttat gaggatctat
   127561 cgtgacagaa tccgcttctg aaatagctct gaacccttca tatacctctt tgtcaatcgt
   127621 tacgcagtgg tggaaaacat gatgttcttc aaagaattca gctaaggaag catacggcac
   127681 aaattctcct gcttcgttta gaaacagtga acgcgcatag ccgccatatg cggcatcaac
   127741 atgtaaatag aaatagatcc cttcatcttt taacctttct cttaattgga cgattttatc
   127801 tacactgtct acttgacctt cttctgtcgt tcctacgaca gcaaccacac ctagaattgg
   127861 gattttttgt ccagctaatt cacgaattgt tttttccaaa gcattaatat ccattcgata
   127921 atcctcttga actgggatag caaccatctg atctaagccg actccgcaaa tatctaacgc
   127981 cttcatccaa gaatagtgtt ttgtttgagg gaccagccat ttccctaatc tttggatatt
   128041 ttttccgcta cgggaagaag ctgctttgac ctcatccatt tcttcgtccg taaagctttc
   128101 ggtcatttcc aaaatttctt caacagataa attcaaaagt tcccactcag acattttttt
   128161 cactttttca ggcagcactt ccttgacagc taaaggaatc gatttgatgc aacgggcgta
   128221 ccataatcct tcaagattag caatagaacc atctgctgca atgtgtcccc atccgtctgt
   128281 catattgaat aatgaagcaa aatcctgtcc tacttctgct tccatttgcg aagttgccat
   128341 agaagattcc aatgcaacat tatttgggtt ccacagcatc gcatagttgt aagctaataa
   128401 agcaggcatc aatgtttcag cattcatttg tccccagtaa cggcctgctg aatgccatgg
   128461 gatcgatcct gcgcgtaatc gctgactgac ttcatccaag acctctatca tatggtcgcg
   128521 ggtggctaga taatctggag tgagcttatc tccttcgcta attgcaggca tatctgaagg
   128581 aatataattt tctctccatc caagatgttc atctaccatc ttgttcaaca acattttata
   128641 aactggtcca ttttcagctt tgtcgcctat aaagacggcc ttgatatcca tatctttcat
   128701 ttttcttccc tccatatttt gttggtcaat gtcttgcaga tacatgtgtt tatctgtaac
   128761 acctcttcac tatagcgcca aaaatgagaa attttttttt ataacatcaa atccacgtag
   128821 atttttacaa acaaaaataa ataaagtcgg atttttctga taattcaacg caaaaaagcg
   128881 aacaattttt caataaatca gtcttctaaa atagataacc taaaaaaagc gaacaaacaa
   128941 ataaaatgac tgtccttcat tcactaaaca tatttttatt tttttatatt gttaaaaatt
   129001 cacaatttat tttttaatag aattatctgt tttccaaatt ttataacata aaaaaccttt
   129061 tttaacggca tttatatgct ttttcagaaa ttaactgatg atttcaaaaa gaaaacatgt
   129121 cataatattt cccatattgt gcaaaaaaat acaaggcgga ggctctacta tgattgaaga
   129181 atatattgag aaaaatattt taagacagct gtttttatgt gggcaattct atgtaaataa
   129241 agaagtcaat ctagaaaagc tctccaatct cctgcatgtt tgcaaaacca ctttactcaa
   129301 tgatatcaat aacataaaaa aagaatttga agaacagatc gcttatacac atcgggaaaa
   129361 ggaccgttac actttatatt tttcagaaca tatacctcga tgcaagatca tgcagcaatt
   129421 gtctcaaaat tcgttgtttt taaaaacttg tctgctttat ttggaagaag atgaacctga
   129481 ttatcttcaa ttaactgaat gtgaattcat ctctgtcagc aaagcttatt cattaaaaaa
   129541 acaagtatta gcttacttta atgactgcgg gatcgaaatc gaccgctact cccctcgatt
   129601 cactgaaatg gaaagacgtc tgctgcttct taatgtgtcg tatcgtttag gcggttttaa
   129661 ttcttgggag ctacctgaat cgttttttga acgtgccgat cgtttcattg aatccgtaac
   129721 ggaaaatagt ggcagatttt atgataaaga aaataaagaa atcttatcga ttggttttgc
   129781 tatcagtttc ctacgccagc aagtgtgtgc tgtgacaata gattcaaaat ttatcgaaga
   129841 aatcaaaaaa cgtcctattt acaattatgt cgaatcagca tgggaaaaca ctgatttcca
   129901 aacctattat aaaaaagaag aattcgcctt tattttgaca ctattcaatc tttgtaatta
   129961 tggtttccat tcttatcagt tgattgcgga agactttcaa cagctacacc aagtgtttat
   130021 tgacaatact cccgaaataa aagaactagt tgctaccttt gaatctcact tcaatcaaga
   130081 attattcgga aaccaaccgt tcgaacgtgc gctgattcat ctgatgcgtt cggcttggga
   130141 taactatcaa ttattcatgc cagaaaaatt ttatctttta aatgaggaac agacaaattt
   130201 atataaagaa gtccagacaa ttttttccag ctggtccagc cagttacctt atgatctacg
   130261 gttgaatcct aattgcatgc gggcttttgt gatcgaattg agcggaattc tccgtctgac
   130321 aaaagaacat ctgactatct atatcgtcac aaattcggat gttcattatc tgatttatcg
   130381 ggaagccttg gaagctgtaa ctactttcga ttttcaagtt gcaccaacaa tttattcaag
   130441 tatatctgat ataaagaagt atgcgcagca gtcctcaaac cgggtattat gtgaacgtac
   130501 attatataca ccagatgcgg tccaatatga aaatattatt cctatttcga tcaatacgat
   130561 cgatcgagct atcatttcag ctgtgcaaaa caaataaggg gctgtgacaa aaatcatgtc
   130621 acagcctctt atttgttgaa gatgcccatt tctgtaacaa tctcttgata cacgtcaggc
   130681 tcacaacctc acttattttg gacatctagg ggtccgatct ctttatttaa aaaataagaa
   130741 atgatggttc ggataactac caacgccgct agttggaaaa tatcctgcaa ggtaggtttg
   130801 ataatcgatt cgatgatccc agctgaaaca agaatttcca aacttagaag tatatagcct
   130861 cccaacatct ttttaatagc attgttctcg cttactcggt cgctttgcac ttttgtatgg
   130921 acaaataaat atttcaaaaa atccttgatt gatagacaaa caccccaaac caataaacag
   130981 atagcaaaga tatcaagtcc cagtacaagt ttttcgaaat atggaagaag aaactgcatc
   131041 aactctgtcg tcatcctacc tctcctcttc tttgtttttc tttgtcttct atttcctgat
   131101 tcaagaaata agaaatgact gttcgaatga taataataag tgcaagcttc aaaatatctg
   131161 tccaagtcgg cttgataact gactcgatga tatcagcaac gactagtact tccaagctta
   131221 acaaaatata acttgctagc agttttcttg tatctgcatt acgagtatta cgttctgcat
   131281 agtttttctt gacatgagac caatcaaaaa gtttgaatag aacaagaacg atcccgacaa
   131341 ttaagatacc gatagaacaa atatttaaaa tagcaatgat ccattctaga aattcaatta
   131401 aatttgagcg aaacatatac gctacctact ttcttttaat ttgttcctat tcttttattg
   131461 taaaagaaag ttaccttgtt cgtctattta aaagttgttc taatgataac ggttcgctta
   131521 aatttttctt tttcactctc ccatttttct ccctccatcc ataaatcgtg gaagtatcca
   131581 aaacaacgta gcaagttaaa ataaggctta gtccgtcatc agcttttcaa agaagcactg
   131641 tctcggtgct ttttttgtac ccaaatttct aaaaatagaa ccatacacct cctcaaatct
   131701 tttcttctta cctaccctat ttttatttac aggaaaatca gacacttctt cttttttgtc
   131761 aaaatttcct ttttcttctc gtcctatcat ttggcattct aataaaaacg aactatcctt
   131821 ggatatagaa agagaaaaca aggaggcgtt tttcatgagt aaaaaattaa tcaaagtcgg
   131881 catcggttta ggtttgcttg cccttggcgc tgcatatcta ggaaaaaaga caggactatt
   131941 cgaagatgac agtcatttat atgatgaatt cgaatcaatc tagcatccta tttagttgaa
   132001 tcactacgga catacttgga agacattgga ggaatcacaa tgagaaaaag taaagcaata
   132061 atgattggtg ccggtattgc caatatggca gcggctgttt atttgattca agaaggcaaa
   132121 tggaaagggg atcagatcac cttttattca ttggatgatc acggttcaaa tgatggtgca
   132181 ccgaccgaca ctgtagtgga tgaatattgg aataaaaacc atccattaga aaaccaaaaa
   132241 ggctatattg ctcgaggcgg tcgcatgctt aattaccgta cctatgtaga tttgatggat
   132301 ctgttaagtc gaataccttc tgttactgag ccagggatga ctgctgaaga agatacgaga
   132361 gattttgatg caaaacaccg aacctttgac aaagcacgtt tgatggaagg tggcaaagga
   132421 atcattgatg gcggaaaact gggattcaat aataaagacc gtattcttct gactaaactg
   132481 attgctatgc ctgattcaga agaagaaaaa ctagacaata tcactatcga agaatatttc
   132541 aaagatgatc cacacttttt tgaaacaaac ttctggttta tgtgggaaac aacatttgct
   132601 ttcagaacac gtagttctgc tcaagaattg cgtcgctata tgcatcaaat gatttatgaa
   132661 ttcactcaaa tcgaacatct agtgggcgtc aatcgaacac gttacaatca atacgaaagt
   132721 attatgcttc ctttgatcga gtacttgaaa gaacaaggat gcaaaatcat tttgaaccgc
   132781 cgtgtcactg actgggaatt caaagaaaca ccgatgcaag atgaaatcac tgtgactggt
   132841 ttgaaaatta ccaatacact gactcaagaa gaagaatacg ttgtggtcga tgaagataca
   132901 gcagttattt tcactaatgg gtccattaca gattcagcta ctttaggaga ttacgaaaca
   132961 ccagctgtag aaaatatgga ttatggtgca gcttctagtc tctggaaaaa agccagtgaa
   133021 cgtttctaca acttgggaaa tcctgacaaa ttctttgctg atcgagatgc aagcgaatgg
   133081 gtcagcttta ccctgacgac gaaaaaccat ttgttgttga atgaaatcac ccgtatcacg
   133141 actcaagaac caggaaatgc cttgaattca tttatttcaa ccacaccgat cacgccgtta
   133201 ggccaaaaag atgtcaatat gtctattgtc gtgcatcatc aaccgcattt tacgtctcag
   133261 aaaccaaatg aaacagttat ttggggttat ttcctttatc caagacgacg tggcgaattt
   133321 gtggataaac agtatattaa aatgaatgga aaagaaatgc tagaagaact gattggacaa
   133381 ctttcaaaag tagatcctgg accagtgaat attcgtgaaa aagaaacaga aattttcgac
   133441 agcgtaataa ataatatccc tgtctatatg ccttatgctt ccgcgttgtt caataatcgt
   133501 gccaaaagcg atcgaccaaa agtcattcct aaacattcga ccaatctggc ctttacagga
   133561 gaatttgtcg agcagcctta tcagatgatc tttactgaac aaagtgctgt acgttctgga
   133621 gaaatcgctg ctttccattt tgcgggtatt cctatgtccc gtcttgtcaa aacaccgcgt
   133681 tacgacaaag atattcctac tctagctcgt gccgcaaaaa aaatgtttga ataatcaaga
   133741 ggttgtgaaa aagctgccct gcatcaagta ataagaacaa ccaggctaaa acagcttctt
   133801 atgtttttga tttggttgga cttattaccg cagatgcaag cttttgaaca ccgtttacca
   133861 agaggttatg aaaaagctgc cctgcatcaa gtaataagag cagccaagct aaaacagctt
   133921 cttatgtttt tgacttggtt agacttatta ccgcagatgc aagcttttga acaccgttta
   133981 ccaagaggtt atgaaaaagg cgtttagttt cgagtatgaa gaaaaatttt gtgaaaatgg
   134041 ttcctcacgt ttttgcataa atttgactta ataccgagaa actgatccct tctgtcataa
   134101 cctcttttta tttatgtctg tttctttttg aaaacaacta ttttttcaac gtttactctc
   134161 ttgccaatcc aacatcatct aacggataca actatttcaa tgattaccat tacactaaaa
   134221 aatcggaaac acacctctcc gaacacaaat acataagact atagaatgtt tcatttaaga
   134281 aaaaaagcgt ttcattatca aaaaggtaag atctccttta aaatgaaatt atctgtagta
   134341 ctaataatgc agacaatttt atgaatctga aaggagcatc tccaatgaaa gcagtcactt
   134401 ggcaaggaaa acagaaaatg gaaatcagaa acgtagatga tccaaaaata ttagaaccaa
   134461 cagatgcaat catccgtatc acagcaacag ccatttgcgg ctccgatctg catctttacc
   134521 atcatggtga agcagttttg gaaaaagggt atgttgtagg acacgaacca atgggaatcg
   134581 ttgaagaagt gggatctcaa gttcaaacat tgaaaaaagg gcaacgtgtg gttatccctt
   134641 ttaatattag ttgtggtcat tgtcatttct gcctgaatca catggaaagc caatgtgatg
   134701 aatcaaatcc agatcagtta tatggcgggc ttttcggatt tggcaagttg aacggtaatc
   134761 attggggtgg tcaagcggaa tatctaagag ttccttatgc agactctact tcatttgttg
   134821 taccagataa tgatcttcct gatgaaaaag ttttatttct atcagatatt ttgcccacag
   134881 cttattggag cgttgaaaac gcaggagtaa aaaaaggcga tacagttgtg attctaggtt
   134941 ctggtccagt cggtctattc gctcaaaaat tcgctgttat ggctggagca gagcgcgtaa
   135001 tcgcagtaga tccagtacag caccgtctag ataaagcggc taattacaat ggggttgaaa
   135061 cttatttctt agaagacacg gcacaagctg gaacagaatt gtatgaactg gctaaaggtg
   135121 gcgcagatgt cattatcgat tgtgtaggaa tggacggatt agagcctgtc aaagaaaaag
   135181 cgaaaaattt agtaagctta caaagcggca caatctctcc attacagatg gcatcgcaag
   135241 ctgtcaaaaa atttggaaca gtccaagtaa ctggtgttta tatgactcca gcttcttctt
   135301 acccgctgca agaatttttt atgcgggata tcgctgtcaa gcatggacag gcaccaatta
   135361 tccatctaat gccaaaaatt tacgcaatga ttgcagaagg actattcgat cctacacaaa
   135421 tcattactca ttcgctgcca ctatcagaag cagcacaagc atatgagatc tttgacaaaa
   135481 aagaagataa aaatataaaa gttgttttga aaccttgaat tcagtaaaaa atacaggtga
   135541 ttttctttat gcccaaaatc aaacgaaata cccatgagat cgaaatagga aaagaagaag
   135601 atatcgaaat acgaggacgt cgcttccgct tctatttcca aaatcgttac acattgatct
   135661 cactggctgt cgatttatta accgggattt tctatatttt tggaagcctt gcttccttaa
   135721 ctaaaatccc cgatcgatat ggcacctatt tttatttggc tggtgctatt ttcttgacga
   135781 ttcgtcctat tctcagaatc gtaaggaaca tttttattta cgacgaaaaa aaagcaatga
   135841 agagaacaag caaaaaaaaa gaggcatgag aatttttcaa atctcatgcc tcttttttca
   135901 ccctatcgta tcactttgat acagacgagc ggtcgtccca ctttcttcct tgaccattgt
   135961 caaaaatgtc cactcgtatc gttcataaaa cgtggtatga tctgtaatta agtaaagttt
   136021 ttcgtaccca agttgatgca tttcttttct gatttccgct aatatccgct tggctatccc
   136081 ctgcttacgc ttttccggtt caacaaacag ggcacaaaga tttggtgtca gatcagggcg
   136141 gttatggaag tcgttttcaa tgacgcccgc tcctccgacg atggtttctt tttcatctaa
   136201 gaccacatac cattgaggaa tactttcctt attagcgatt ccagtacgaa tactttcctc
   136261 atacacaaca gcaggaactc cccattttga tgaaaaccaa ttcgcgcttt gttttactaa
   136321 ttctgggtgt tctcttaatt tgatccagtt atatgtaacc aatgtttaaa cctccgacta
   136381 aaataaaacg tttacaaaaa ttatacaagt ctttatgaaa attagcaatg acaacaatag
   136441 tgatgtaaaa aaaaaaacgg aaaacaatca cttgttctcc gttttttgat taatcaagca
   136501 tctaaagctt cttttcttct ttcttggatc gtagctacct tgacgaacgg tagatagatc
   136561 aaaatagaaa gaaccaaatt gacagcggct agtacaccac ctgcaatact ttttgttgcc
   136621 aatacaccgc caataattgg tggagttacc catggaggca taacggtagc tgctggcact
   136681 aaacctgtag aagtcgcaaa gtaagcaaca ctaactaaga ccattggtgt caaaataaat
   136741 ggaatgaaca tgatcgggtt caacacgata ggtaacccaa acatcgttgg ttcattaata
   136801 ttgaagacac ctggcgcaat cgacaagttt gttacaacca tatatggttt gtttttacgt
   136861 cctactaaat aaatagctaa cagtaaacct aaagttgcac ctgttcctcc taagttgaca
   136921 aagctgtcaa agaaaggttt gttcacgata aacggtgcgc tatgtccagc tgcgattgca
   136981 ttaacgtttg cttcaatggc tggcgcattg attgtttgca tcaaaggatc gaccatattc
   137041 gctccgtgta aaccaaagaa ccataagaat ggtgtgataa atgcaagcag taaggcagat
   137101 ggataagagt ttgcaagtcc cataaatggt tcttggacca ctgtatagaa agaacctacg
   137161 atatcatcaa caccgaatgc agcaaaaatt gcagcaacta aagcaaacgc agagatagtg
   137221 atcattgctg gtagtaatga agcaaatgct tttgcaactg ccggtggcac accatcaggc
   137281 attttgatga tcaatttttc atttccgctt aaacgtacaa agatttctgt tgaaataatc
   137341 ccaacaataa tagcaataaa caaaccgttt gagctcatac ctaatgctcc gcctaaagcg
   137401 aagaaagagc ctaaagaaat cacacctgcg gaaacaccgt cttttcggta gccgttagct
   137461 aaatgataag caagcaagaa agcaatcaag acagaaagaa tggcaaatgt accgttccaa
   137521 gcagcagccc cgaatccttt ccatgattca ccaccaaaaa ttgagttcat caattcttgg
   137581 aacccgggaa tcggtaagtt attgatcatt actgctaatg ctcctaagat cattaacggc
   137641 atactcacca taaatgagtc ccgaatcgca actaaatgcc gttgtgcacc aatttttgat
   137701 gctacaggta taaaatgttt ctccataaat gcaacaaatt tatccataaa tcttccctct
   137761 tttctttact gtagattacg cgttcaatcg acgatacaga ttaatgatct cttttgctaa
   137821 atctgtaaaa gctatagatg tcatcagatg atcttgactg tggaccgtca aaagcgtgac
   137881 ttcaacgtga tttccttgtg cttcttgtgt cagcatctct gtttgagagt gatgtgcttg
   137941 gactaaagat tcctctgcat ctttgatttt ttcatctgct aaagtgaaat cacctttttt
   138001 cgcagcagca atcgcttcca ttgcatcgct ttttgcattt ccgccaaaca tgatcagacc
   138061 catgatggct tctagatttt tttgatcttc catttattgt atcctccgat aatttttatt
   138121 atttgtttat tagtttttct gcttggtcga gtactttttc accattcatc atgccatagt
   138181 cagccatatt gattacatct aaaggaatgc ctttaggtgc tagcttttca gcaaattgat
   138241 tttgcataaa gcgtacttgc ggtcctaaaa gtaaaacgtc tacattttta gattctagtt
   138301 gattatccgc atctgaagca gatacagcaa agatgtctgc ttccattcct tttgcttctg
   138361 ctgccttttg catttttgtg actaggagac ttgtactcat tccagctgaa catacaagca
   138421 tgattgtttt cttttccatt ttaaacacgt tcctttctat acttacactt gtatataagc
   138481 aagtagcgtg ccaactttaa aagcgcttta aaaagttgat atcatcacat ttaaggtcta
   138541 taccaagttg catacacact aaaaaagaaa gcgcacacta aacacacaaa ttaattttta
   138601 ttaccttttt tcttttaacg tgcacaaaaa aagttgtgac acaaaacttg tcacaacttt
   138661 tttcaaataa acggtattca aaagcttgca tctgcggtaa taagtccaac caagttaaaa
   138721 acataagatg ttgtttttac tttgctattc ttattacttg atgcaggaca gctttttcac
   138781 aacctctcaa taaacggtgt tcaatcagct gaccctgggt attagctcaa aacgctggat
   138841 tcacaacctc ttttatttca aataaccagt aaatacaatg aacatctaca aaaaacttct
   138901 atttatttat agatttcgtc aggcaaatcc aattctctct tatcaatcaa agtctgatac
   138961 cacgaagcag attttttcaa tgttctgctt cgttcatcgt tcaattcgat tcgaactaat
   139021 ccatatcgat tcttaaatgc attcattggt gacacacagt cagtgaatgc ccataacata
   139081 tagccttcgc aatttgcgcc ttcttctgtt gctttgatca agtaatgcaa atgctgtccg
   139141 ataaattcga tacggtaatc atcttgcacg attcctttct catccatata ctgttcttca
   139201 tattctcttc ccataccgtt ttctgttatc agccaaggga tattatgata attttctttt
   139261 agatacatgg ctatatcata catgattttc ggatagattt cccatcccct ggaattattc
   139321 attttcttcc caggtaaatc aaagacttca taatatcgtt caggatggaa aggtgtctct
   139381 tcattccatt gataacgagg ggcgcgaacg cgtttaggat aatattgatt gattccaagg
   139441 aaatctactg tatgatgctg aatcgtttct aaatcctctg ttgcaggatc gaaataaatc
   139501 gattgttttt gacacaactc caggaactct tctgtgtagt atccttttac catcggatca
   139561 aaaaatactc gattataaaa caaatcatac atataagcag ctttttggtc ttctggagaa
   139621 ttggatcgag catagaccat ttcaggattt aaaatacagc caatccttcc accataattt
   139681 ccgctgcggt aaatctctac tgctttagcc gttgctaaaa ctttatgata attccacatc
   139741 atccactttt ttgtgttttg ttcatgcggc cagcgaacag catccaaata gcaacgcgtt
   139801 tggggaacga ttggttcgtt gaatgtaaac cagtttttta ccctatcacc gtaacgatca
   139861 aaggcaattt gagcatactt agcataaaaa tcaactactt ttcttgaaga ccagccatca
   139921 tatttttcca aaagaaaagc tgggacttca taatgttcca aacaaatcat cggttctacg
   139981 tctgcttcga tcaaagagtc gatcatttca cttatatgct gagcatattc ttcatctact
   140041 atacagttct cataatcagt aaaaaaacga gcccaattga tagaagtccg ataatgtgtc
   140101 agatggatgg atttcatcag atccacatcc tgctggtatt gttccataaa attagttgct
   140161 acggctgggc catacccatt atgccaaaca aaatgttcat ttttatacca gctatctata
   140221 aacgaatcct gtccttcttt ttttcctttc caaccttccg tttgccaagc agaagatgca
   140281 gcaccaagaa taaaattttt tggaatagta attttcatga tttcctcctg ttttttagat
   140341 cccatcattt tccacacgat ttgctacttt tacaaatggc agatagatca aaatagaaac
   140401 aataatacaa acaagttgcg taataactgc accgatattt cctgctgttg ccagataagc
   140461 attgacgatc ggcggcattg gccaagggac cattacaact gctttcgccg caaatcctaa
   140521 agcggttgca aaataaccaa tcgttccagt gataagaggc gttaagataa acggaatagc
   140581 taaaattgga ttcagcatga tcggcatacc aaaaataact ggttcattga tgttgaagat
   140641 cccaggagca agggataact tggtgatttg tttcatatct tcccgttttc caactaacaa
   140701 tactgcaatc agtaaaccga tcgtgactcc agacccgccc atactcatat acatatccca
   140761 aaatggcata gtaataatgt tcggtacttc ttttccagct tcaaaagctt ccgtatttac
   140821 agcaattgcc gctaaaagca acggttcccg tatcggcttc accatttgat ttccatggat
   140881 accaatcacc caaaagactt gcgcaataaa catcaatagc aaaatacccg gtaacccttg
   140941 tacgacacct tctaatgggc gttgtacgac attgtaaatg atatcataaa ggtacattcc
   141001 tgtaagttct ttgatgataa atcccgcagc cgcgataccc gatacagtca gaatcgtcgg
   141061 gaataaagca gaaaaactag cagaaacatt cgttggcacg gtatcaggca ttctcagctg
   141121 tagtttcttc tgtttcctca accagcaata aagttcaata gaaaggatgg agaccaacat
   141181 tcccaaaaat aaccctttcg tatctgtata ttgtttagcc aatacgtttt ctactataac
   141241 agtctgattt tcattcacta attcataaac agttggatta acagataaat aagacatcac
   141301 tgcgatgata ccagggaata caccatccac tttattcagc ttggctaatt cgatccctat
   141361 cagaaaaaca gcatagatgg tcaagaaact tagtgtgaca tagctgatgg cagatgcgat
   141421 aggctttaat tcttctaaaa aacgcaagaa acttatctgt gctaagccat tttccgagtc
   141481 aaagaccatt gctgaaaaca atgtgccaaa agcacccgta ataattaccg gaatcaatgc
   141541 agcaaatgcg ttttttatca ccatgatata ccttaaatta ttgattctcg ttgccacgtt
   141601 ccctaaaaca ttggctaatt tatccataaa actcattcca gatcccctca ttcctgcttc
   141661 ttctccttga cgtcatctct ttttgtctat tagtcagcgc ttacatttcg atgaacagaa
   141721 actatttact cctctttttt accaaatata aaacaaaaat tcatctgatg tatttatcat
   141781 aaaatacatc agatgaactg tcaattagtt tctttttctc ctattatttt ctgtaattct
   141841 ctccgattta ttgccatatg gacagtcatt gcatcaaacg cagcaagtgc attccgcctt
   141901 tcgatggcct gaacgatctc tgtatgaata tcgatcgtct cgattttcag atgctggata
   141961 tcataatttt tattaaacaa agcaattgat tcattaatga ttgggataat atgcagcaag
   142021 gctacgtttc cgctagcttc tgcaatcatc gtatgaaaat cgatgtctaa ttgaacatgt
   142081 tcttgtgtgc cttctcgaaa agaagtttca atcttttgcg ttaatttttt catttggacg
   142141 atttgattct tcgttgcata ttccgctgcc aaacctgcca tcttaggctc caataaatat
   142201 ctcagttcaa ataagtctcg tatcatccga tcctgatctt caatcaaaga aaaaccgagt
   142261 gggtcattgg caatccctcg cttatcactg acaaatgtcc ccgctccttg ccgaacttca
   142321 aggatattcc tggaagctag agcccgcaca gcttctcgca ccgtgcttcg ccccacctct
   142381 aaataatcgg acaattcata ttcattcggc agtttatctc ctacttgcaa ctgcatttcc
   142441 tttaaatatc taaggatctt gtcagtagtt acttctacta gggttttatt ttcttccaaa
   142501 ccacatacct ccttttcatc tgatgtattt tttcgtaatt attataacac gtttgaatca
   142561 tctgatgatt ttttgtatac aaaattgatt aatccgttca tagacataca aacgaagaag
   142621 atatttacca taaaaaagac taggataaaa ttcactccta gccttcgatc atttttattt
   142681 tgaacgacaa aaaatatgat gttcaataac agacgatctt cattcgattc ttgatgtcat
   142741 cttttgtttt ttttaatata tggataaaaa gtttgattac atcgtttcta ttctagaatt
   142801 tggtcgattc tcaattcttc ttctggtgag aaatctaaat tatccatcat tcgaacactt
   142861 tcttgtaact gacttaaccg acttgctccg accagtacac ttgtaacaga tttttgtcgc
   142921 aaattccatg caagggccat ttgcgctaat gattgtcctc gtgatacggc gattgtttgc
   142981 agtgctttga ttttttccag agttgatcca acttgttctt ctgaaaggaa tggtatttcc
   143041 ttacgatgtg ctctagaatc ttcaggaata ccatgtagat aacgatttgt cagcaaccct
   143101 tgagcaagcg gactgaacgt gattgcccct agcccttctt cttctaatac tggagataga
   143161 tcgtcttcta gtagtgcacg agaaaacata ttgtagcgca tctgatgaat aatgaacggt
   143221 gcttcctttc tttttaaaat ttcagtcatt ttttttgtat cttctccatt atagttggat
   143281 ataccaatat ataatgcttt tccttgtctt acaagttgca tcaaagcttc tgctgtttct
   143341 tccaaaggtg tatcagggtc tgggcgatga tgatagaaga tgtcaacata atctagcccc
   143401 attctttgga gactttggtc acaacttgaa atgatggatt tacgagagcc ccattcccca
   143461 tatggccctt cccacatatg ataacctgct ttgctagaaa taatcagttc atcacggtag
   143521 gcttgaaaat cttctctaaa aatacgccca aagtttttct ctgcagctcc agcagggcct
   143581 ccataattat tcgcaaggtc gaaatgggta attcccatgt cgaatgcgcc tcttaaaatc
   143641 tctctttgat tatggatagg atcataatca ccaaaatttt gccataatcc gagagataaa
   143701 atcggtaatt tcaaacctga atgaccggat ttacgatatt ccattttttc ataacgtgca
   143761 ctatctgcaa tatacaattt ttctcctcct catcaaatct ttttaaaacg ctttcttttt
   143821 tctattttta gtttactaca ttttaaagaa aaggagttaa tcaaaatgat tttttcgtat
   143881 atttttttgc catttgtttt ttactcgttg atagatcaga aattgattac gtgcttgctg
   143941 gattttttta taacgtttcg gttgtttttt gtaaaattgc tgatgatagt tttctgctgg
   144001 ccaaaacgta ccggctggtt cgatttttgt gacgattggt tggtggaaag ttcctgaact
   144061 gtctagtttc tgttttgatt gttccgctaa ttctttttgt cgttcgactt gataaaaaat
   144121 cactggacga tattgcttcc ctctatcttg aaattgtcca aatgtatctg tgggatcagt
   144181 aatttgccaa tagattgtca gcaactcgct ataacttatt tctcttgtgt cataaatgat
   144241 ctccacagct tccacatgac ctgtgtaacc accacttact tgatcataat ttggtttttc
   144301 aacatgtccg cccgtgtatc ctgacaaaac agataggatc ccttttttcg attcaaacgg
   144361 ctcgaccata caccagaaac atccccctgc aaatactgca cgttcttgat agtcagcatc
   144421 ttgcatggca tgcttggaaa agtcatattt ttgctcctta tcaaatcctt ccgtaatcaa
   144481 taaataaaag tccattacat caggcgtcag attcattcgg atagctaatg gacgtagtgt
   144541 tgcttctagt tttgataact gttctttaac ggatatattt tctcttatgt cattctttgt
   144601 ctgaatcaat acttttcgtt cccaatctcg tgtattcgga ttcaaaatca gattataaag
   144661 tgtttgaatg aatatttgtt cctcatccat agattctcct cctttccgaa gaagacaagc
   144721 tagtgacttt agctgtaatt tatcataaag tgaggaaata agcgattact tcatccccct
   144781 acagattttt cgtataaaaa agagagaatc cttatcgaca gattctcctt tttaatcaat
   144841 ttattttata aatgaaaagt taatttacct tttatggaaa actattttaa aaaataaaca
   144901 taaatatgca gttaatgtaa caagataaga aataatagtc gttactgtat aacttgtgct
   144961 tactttaata tcagcaactc ctttgactcc gcttatttgt accaatccat tagcattcac
   145021 ctgctccttt acaggttgat gatttatttc tacattcgtt cctaaataag catatacagg
   145081 aagtgtgatt acttggctct tctcattagt tactctatat atgatagaac taccagttgt
   145141 tttataagga aggtttaccc attgactgcc aatctttgct tcgtgtttta ttatatgctc
   145201 cttattctcc aatgaagcga taggagaata atcatataaa aaataatcca ttcctttagt
   145261 ggtatacgcc tctactgttt ctttgtttac aacaatatgt cccggctgag aaattatttt
   145321 ttgattgaaa ttttttttac tagaaacagt taaagtgatt aataacaaaa cagtcataaa
   145381 taaataaaat agttctcttg tcttattgta tcttctattt ttgaaaaata tcatcgttaa
   145441 tatcagagaa gagaatatta tttgcaagct taagatccgc caaggaaatt gtaacaattt
   145501 ggctggcgta ttttgcagaa gataccatgg caccaaacta gttgtagcta gccaagtcaa
   145561 acacgtacca taataaatct ttttttctat gtttgttaat tttttaaata aaatcagggg
   145621 aatacataaa gataacagac caactagtcc cattgagtaa gtagttaagt cgttgttcat
   145681 agcttcagtc agactatcga acacattaga tgcatgagca tataaatttg tcacgctagg
   145741 tttgttgatt ttttggtaga gcatttgttc aaacatcgga taccaaaaag cactcgtcag
   145801 aaccaatgtc attcctgccg cactaaataa ttttttaaat atagcaataa aatctgcatt
   145861 cttgaaacct ttagaaacca aacgaataaa aataaaaatg ataattaatg ctgtactcat
   145921 taatgttgat agtacatggc tgtacactag caaactcata ctcaatgcta gctttacaga
   145981 cggatacttt tcttcttttc cagagatgat ttgataagca taaaataata cagggataac
   146041 aagtgttatt gcgatagctt cggcaattgc ggaacgatag tagatgtcta ctgtccgata
   146101 agcagagaaa atatagagac aactaaataa aaatgccgct tttttttgtt ttaaaaaccg
   146161 ctcgccataa tgataagcta cgagtgttgt acacaagttc aataaccaaa cgtaaataat
   146221 atagcttaca atcaaatttt ttgtcatcca atataacatt acagctggaa aaaaagtcag
   146281 aaagggataa aaataattta caccatatcc agtataattg aatgtctcat aattaatcgg
   146341 actggtaaaa atagtcgata accccttgat tcgagataag tgaaaagaaa gatctccagt
   146401 ggtataaata ctctgtgtat ttttgaaaaa gatgaaagaa tatatactac taactataat
   146461 taatccactc aatattacta cttttttatt tattttcatt cttcttgttt atctcctttt
   146521 tagtcacata tcgagaatct ttcaacttat taggtgcaaa tcgtttaaaa cttgaataca
   146581 ccactaaaca ataagtcaga atactgatcc ataaagccat gagagcagtt ggagtatatt
   146641 ttgttgtaat ttctatctta ttttctccag catttacatc cacattcact aagccatgtt
   146701 ttttataaga gtcatttacg actttaccgt ttacttttac ctgtgtacct aaataagcat
   146761 atactggtac tgtcactttt tgctttttgt tgcttgccac tgtgtaagta atgttattat
   146821 ctgcagcttt atacttccct ttttcccacg attctcctac ttttacttca tgtttttcta
   146881 agtgaggcct ttcttttatc gcttctgttg gggcgtaatc gtataatcct cccatatctg
   146941 atgttgtata acgtgggagt gtttcttcat tgataacaat atgaccgtgt tttgattgga
   147001 taacttttgc gtaattttct tttgctgcta tagacgtgac gagtagaaac accacagtag
   147061 cgccaatgat taaccatgaa tattttttat tcatagtctt tttcataaaa acaagcgtta
   147121 tgatcattga agaaaaaaag atttggatac ctaagatacg ccaaggaaac tggagtaagc
   147181 ttatcggtgt tttttgaaac aaaaaccatg gaaataaatt agttacgcat acccatgtag
   147241 aaactacgcc aacatagatg attttttctt ttttctccat ttctttcagt atgaataaag
   147301 gaaaaatcaa cgcaaccaat cctaataacc ccatagtgta tgttgtaata tcgttattta
   147361 acgcttgaat gatactatct gaaatagaaa gtgcttctag ctgcaaatct gttttgaagg
   147421 gactgtgtat ttcttgataa ttcatctgct gcaacatagg ataccaaaaa tagctagtta
   147481 atgcacaagt caaaaaaaca gcctttacta aattcacgac cacaaatttt atatcgtttt
   147541 ttatttgctt gcttaaaaac aaacgaacta taatcataca agcaatcaat gcactacaca
   147601 atagtgtagt taagacatga gtatacacaa gtagacttat actgacagat aaagcgatga
   147661 cttttttttc tctattgaat atcaatccat aagcataata taaaacagct gggataatcg
   147721 taagagcgat tgcttctgca attgcggatc gataatagat atcaactgtt cgataagttg
   147781 caaaagtata taaacaacta aataaaaaag cagcatctat ctttttaaaa aacttttctc
   147841 cataataaaa actaatcaga atcgtacaaa tatttaataa ccaaatatat agaacataac
   147901 ttaatattaa gttatttgaa attccataaa aaacgactgc aggaattatt gtaagaaaag
   147961 gataaaaata gttcagtcca tctccataat tgttaaatgt tgtatagttg attggccctt
   148021 caaaaataga agataaccct ttgattcgtg aaagatgaaa agataaatca cctgttgtat
   148081 aaatttcttg agcatttctc ataaaattta ctgaatataa agtactaagt aagataagaa
   148141 ctactaaaag aaatgctttt ttacttattt tcatagattt gttgttctcc ttttttaatt
   148201 ctattttcag tatactagaa ttgtcttttt ttaataagtt ttgcgttaaa tttttcatag
   148261 cactagtatg atatactaga aaagaatcaa tttttattac ttttaaatag gagaaaaaaa
   148321 tgaaaaaaaa caaaaaccaa ataatagata tcatttttat gttatttcta agttttgctt
   148381 acgtcatccc tctattcatg tctaagggaa tctatcatag tgtcaatcag gatacttact
   148441 ttcatttatc tcgaatcata ggactagata atgtatggag ttctccagta aacttcaata
   148501 acttcgatca tcatggaaca atgatgaata ttttttatcc atggcttact ctttatccag
   148561 cttttttatt ttataaaatg atgggaaatc ttgttttaag ttataatatc tactattttt
   148621 ttatcacttt tttaacaatg gtcgtttctt atttttctat gaaacaaata aaaaacaatc
   148681 gttatatttc gctgttattt tcgattattt atacattttc cgcctatcgt gccattgaca
   148741 tattccatcg tgcttcattg ggagaagctg tcgctttaac atttttgcct ttgatactaa
   148801 tggggtgtta cgaaatttat attagagatt accaaaaatg gtactggttg tctattggca
   148861 tgacgctagt agtgtatact catttacttt ctgtcgctat ggtttctgta tttattggag
   148921 gaacgttgtt tctttctttt tatttttggg accaaaaaat cgctagactc ttaagtcttt
   148981 taaaagctac cgtcctcacc ttttttttaa gtgcgggatt cttaattcct tttattcaac
   149041 aatctagagc acaagaattg aaagtcccac tgggaaaaga attaagtggt atggctccta
   149101 gtgatatgct gacacatatt ttaaataata actataacaa ctatactatt ggattattct
   149161 tatttctcgg attgattggt gcttttattt ttataaaaaa attaactgct gatgatttat
   149221 ttattttctt tcttggtgta tttgttcttt tttgttcaac caatttattt ccttggcaac
   149281 tttttaacca tacaccagtt aagagtctac aatttgtctg gcggttaaat ggtttttctt
   149341 ctttatttat agcctatacg atgagtatag tgatctatta tattttttct actaaaaata
   149401 attcatggaa aataatggtc tttacgtttc ttgcactaat attgcatctc tcaggagtaa
   149461 gcaattctat ccatgctaac aaagattcat taacacttat agcacctgaa gacgctgttg
   149521 ctatagctga aaattacaat catactgatt atgcaaacaa agagtcaatc tatcatcctg
   149581 atatgattaa taatgatcaa tatttactag gtaaccagga aataaatcca acaactcatt
   149641 ttatgagtaa caaacttaca attgaattag ataattcgaa taataaaaat actgtcttga
   149701 cgacacctat ttatcgttat aaagggcaag tggcttctat taatggcaaa ctagtacaaa
   149761 caaaattatc taagtttggc acaactgaat taactattcc tccaggtata aataaagtcg
   149821 ttattactta tcagtacaca aaactagcaa ttgcttcaag atatttatct atcgttactt
   149881 tgattttatt tttactttat cgatttactt tttctaaaca aaaacaaccg agaagagaag
   149941 tacaacattc tcactagatt tttatttaag aaagactttt tagctgtaca cataaacagg
   150001 ctaaaaagtc tttttttata tttctttaat ttgcttcata cattatttaa catcgatatt
   150061 tatactgtga aacatgcgtt tcatccatta tcaaagtatt tttatcgata ataaacttca
   150121 tccaaaaaca gaccttgtgc agggacggtt tctcctgcat gttgccgcac tttgctttca
   150181 aatatttcat cgattgtttc tatcggcatg gcacctgtgc cgatttctag taatgtcccc
   150241 atgataatcc gcaccatttt atataagaat ccgtctccga caaaagtaaa atgaagcatt
   150301 tctccctttc gttcgataga taattcatca atcgtacgca ctgtggattt ttttgatttt
   150361 ttcaatgcag aaaaaccaat gaaatcatgt gtacccacta atttttcgca agctgcattc
   150421 agcttgtcca tatccaactc ttgagggaaa taaaagctgt ggtttcgctc aaatgctgaa
   150481 ggaatcacgt tgttccaaac ataatagctg tattttttgc ccaccgcatt atatcttgaa
   150541 tggaagcgct caggcatttc ttcgattctt ttgatgacaa tatcccgcgg aaggtaacga
   150601 ttcataaagt cgatcatttc tgccagtcca atttctgtgt ttgttttaaa atttgctact
   150661 tgtcctcgcg catgggtgcc agcatctgtt cttcctgatc caataatttc tatctcttca
   150721 tttgtcatct gatgcaatac tttttcgatt tttccttgaa tggttttatc gtcatctcca
   150781 agtctttgcc atcctgaata tctttttccg tcatattcga ttgtcaactt gatgtttctc
   150841 atattttagt ggctcctctg tcttccattt ggtcttctct ctaccttttt aagattgtag
   150901 cacaaaatat agctccttgc ttctctaaga cagaagctat acaagaaaac tgtggaaata
   150961 tatatttcag aaaaacaaaa aacagccacc ggatgaagta tccgctgact gttcaatttg
   151021 acggctcaag ttttactgtt tgagatgcaa gatactcaac ctggcaatgg gcacataatt
   151081 aaagaaaaaa ctgattcttt ggcaaaaacc tattttattc agtaatggcc aagaatttat
   151141 agaaggaatt cttgccagtc cataaatcgc cgcaaaaagc aaactgatca cagacagtcc
   151201 aaggtataag tggcttacgt ccatttgccc attttttcga tagatcaaga aaagaagaac
   151261 tggaaacaat aaaaaaccac tgtaacctaa acctgatccg atattatgga cccatgtcga
   151321 gacattccac gaagattcat gggtattgat actgaacaaa cctgtaaaga tacaatcacc
   151381 tataccatag aacgcgatgg ctgctgtaac tagggtagct aacacacgag atgtactaac
   151441 aaatgcagca tacaatgcag gcaatgacag gatgaaaaag atccctgaaa ccacagacca
   151501 aataagaaac gcttttctta caggactgga aacctctcca aatacactga tcacgctctt
   151561 catttgatct aattcaggat aatacaatcc taatacgtac ggagtcaaaa aatcactcag
   151621 cacgcctaat aataaaaaga aaaaaccaaa tttacgtaaa aaatccatga tacgcctcca
   151681 ataacaatta cctatgcttt taattgttat tataaacgac tttatcttga tttaacagaa
   151741 acctctattt acacgaacat ctatataact cattaaaaac tcgaatcatt ttaaaaatac
   151801 taaatatcta acgttcaacg ttcacttaat ccatctaaac aatctttcaa tttgattttt
   151861 tccccacttc ttttacgcgc gcctagctct cctattgcag taaacaacag atcacttgac
   151921 gaatttaagg cagtttccat tgaatcctgg ataacaccga taataaatcc aataccgacg
   151981 atttgcatcg cgacatcatt agagatacca aacaaactac atgctaatgg aatcaataac
   152041 agtgaaccgc ccgcaacacc tgaagctcca caagcagcta aagcagacaa aatacataaa
   152101 aggactttca aaacaaaagg tgcttcaatc cctaaagaat gtacagcagt caacgtcatc
   152161 aaggaaatgg taatcgcggc accgcccata ttgatcgtag cacctaacgg gatcgatatg
   152221 gaataagact cttttgtcaa tttcaatctt tcagccaaca tcatgtttat cgggatattt
   152281 gcggcagaac ttcgggtaaa gaaagcggga agtgcgcttt ctttcaaaca aaagaaaatc
   152341 aatggatagg gattttcttt aataaacaaa aacgtgagga atgggtacac aactaatgcg
   152401 acaaacagca tcgtaccgat caaaacaagt aaaagctgca gatactctgc caatccagcg
   152461 attccagtcg tagcaattga ttgataaacc aatcctaaaa tccctatcgg agcaaatgca
   152521 ataatcactt gtacgacttg tgacaacgct atagatatct gatccaccaa ctcttttgtc
   152581 gattctgaac gtgctcgaaa agctagtccc aacaatacag cccagaataa gacgccaagg
   152641 taattcgcat caataagaga ttgaacagga ttttgtgtga cattcgtcaa catagtagat
   152701 aaaacacttt ccaaactttg cggtgctttt tcagatactg tttcttgtaa agtaatagga
   152761 acagtaaaaa ttgaacttac aataactgct acaagtgcag aaagcaatgt tcccactaaa
   152821 tataagatca aaataggctt cacgaatgtc ttattcccaa ttttatgctt ggcaattgac
   152881 gccatcgtca agaaaaaaac cagaataggt gcgattgcct tcaagcaact gacaaacaac
   152941 gttcccaata ttgaaataaa tgtccatgtg gggaaaaaga taccaaacaa tattccgact
   153001 aaaataccaa caacaatacg ttgaactaac gacatatcca aaaacttctc taacaaatga
   153061 ccaccctttc acaattttaa caatcattgt atcattttat gcaaagaaac taaaggtata
   153121 attttctaaa aattctaaca atacaaacaa ccatttcttg ctaattttgt actttttctg
   153181 tctacacgtt gttttttgag atattctaag ttaggatgtt attaatgggc aacttatcag
   153241 gaaacgtttt cttatgtttt tgtccaattt ttttgtgagg tgtttattct tgaaaaaaat
   153301 aaagaaggag agttatggct tcctgacaat ctttgtcgct tgtataatga tggttttaca
   153361 gcgtatattg atttcagcag tcactattcc tacatatgca acaaaccctg gtcgattcta
   153421 catctataca tttttacaaa cttccgttgt tattggtgca aatttgatcc cactttacat
   153481 gggttaccaa tatcaaaaaa tcgcccgttt aaaagtttta cattatttaa gtcgttttgc
   153541 ctttatattc ctaatagcca cactagtttg caatatcttt ttttacgtac aagctggcag
   153601 cttgaatatt cgtgattatt ggcttatcct aacacctatc agtcagaatt actttactta
   153661 tgctgtttct tgtactttgc ttttttgtag tattccttat acagttggat ttttaaataa
   153721 attatcgaaa acaacaatta aatatctact attattttta acaattggat tagttggctg
   153781 ctctactcta tttaataaag acccgtttgc attacagaat ggacagagta ttttatggat
   153841 tttttattta tttttaatgg gttatggttt gaaagaatgg aactggaaag aaaaggtccg
   153901 ctataagctt cctcaagtat ttatcagctt gattgtttta ttcggtttga ttattttgat
   153961 gacacaaatt tcactcatca tcagaggaaa tacagataca gcgctgaggt tttctaaacc
   154021 atattctctc ttcgcaatgt attatacttt ctcttcgttt ttatttttag aaatgctatc
   154081 tgataaatta ggtataaaag gaagaggaaa ttttcttgct gtatttttga ttgtcactca
   154141 agttatatgt aatttccctt tagtttctta tcgcgtatct acatttatga aacgtgaatt
   154201 tccaaactca gggaaggcat ggctgataaa tatcgggcag ttttttattt gatatttatt
   154261 agcagtgtcc ctcctagtta tctttttatt ttatatacaa aaaatccgtt tcattcatcg
   154321 tttcatcgaa tacttcgctt ttgattcgat tgaacaatta actcagcgtt ggaaaaaaag
   154381 aattcattgg ctttttgttc ataaaagtta ttttttagtt gcagcatttt tttactgctt
   154441 taccttcgtg cagattttcg ttttagaacc aaaagaaggt tggaaagaaa ctatccaggt
   154501 tgccttaaaa atatttactc agcgacaagc acccatgatc ctgacaatca tcattattat
   154561 gggatttttc ttcttactat tgttgatttc caatcgtttt tggtacgcct tgactgctac
   154621 cttgatcatt aacttactgc tcactatttc gacagtaatc aaaatggaaa tgcgggaaga
   154681 gcctgtcttt ccttcagatt taaaaatgct gaccggattg tcagaactat tatcgatggt
   154741 tagcccagtc ttattaatcg tgggcggatt gatccttctc ctattattga ttactagtat
   154801 tattgtacaa cgtagactac aaaaacagta ttccttgaag attcattgga aacgtcgatt
   154861 tatcagtatc gctgtattat taggatgttt ttcgggtgtc tttttcatta atcacaaaaa
   154921 ttcgccttct ttcttgctgt ttaatttatt taaagtaaac aagactttct ttaaccagca
   154981 aggcgccgta aaagataacg gaccagtcat tcaattccta aacaatatcg acattaaaat
   155041 catggaagaa cctgctggct attcgaaaga aaaaattaat ggaataatga aaaagtacaa
   155101 cacaaaggca gatgaaatca accaaacaag aagtgactgg ttaaacaatc agacgatcgt
   155161 tctcaattta agtgaaagct tcagtgatcc tgcacgtgtc cctaatctga cagtacctac
   155221 agaccctatt ccttacatca aaagcattat aacagaagga acaggaggat tgatgttatc
   155281 cgtcggttat ggtggcggaa cagctaacat ggaatgggaa ggactgacag gcttgagtat
   155341 cagtaacctg tctgcttcgt tagttacacc ttatacccag ctagtggagc gtcagaaaat
   155401 aagtccaaat attaccgatc tattcgatga aaaaatagcc atccatcctt ttacagctgc
   155461 tctttatcgc agaaaacaag tgtttgataa atttggtttt gacaggttct attatttaga
   155521 cagtccggat aaattaactt atacggataa aatagaacaa agcccacgca tatcggatga
   155581 ttcagcatac aaagaaacct tgaaagcttt acaaagcaat acggaaacgt cacaattcat
   155641 ccaattatcc actatgcaaa accatatgcc ttatgatgat tactatagtg aattgaacta
   155701 cactgctgaa ggaactgctg tcattcctaa tagaagaaaa gaattacaaa cttatatgca
   155761 aggccttcac tataccgata cagcagtaaa aaaattcatc caagaaatcg acaaaatcga
   155821 taaaccgatt acctttgttt tttatggtga ccatttaccc gcaatctata gtgggaatga
   155881 ttcgaaaaaa tatggattag aacagcacca gacagattat ttcatttata acaacaagta
   155941 tagtcgtgaa caagcagcta cgttaaataa agaagttgtt gctcctaata actttggtgc
   156001 tatggctctt gagcatgcga atatcaaagt aactccttac tatgcgttgt tgacagaagt
   156061 atctaatcat ctacctgcat taacaattga tcctacagaa agcttgtcta atcgttttaa
   156121 tggaaaacag gtctttgtta cagaggatag tcaaattatc caagaaaaaa atttaactga
   156181 gaaacaaaaa gaaattctag aagattatcg tctgatacaa tatgatcttg ttgcaggtaa
   156241 ccagtacagt gcaacatggg cgacacaaaa aataaaagac taacaaata
//
LOCUS       DAJSAW010000002        99662 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJSAW010000002 DAJSAW010000000
VERSION     DAJSAW010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 99662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 99662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..99662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.002"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            43..480
                     /locus_tag="ONT64_000137"
     CDS             43..480
                     /locus_tag="ONT64_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301582.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            575..1459
                     /locus_tag="ONT64_000138"
     CDS             575..1459
                     /locus_tag="ONT64_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCR2301583.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1683..4850
                     /locus_tag="ONT64_000139"
     CDS             1683..4850
                     /locus_tag="ONT64_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301584.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4870..5415
                     /locus_tag="ONT64_000140"
     CDS             4870..5415
                     /locus_tag="ONT64_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HCR2301585.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5443..5898
                     /locus_tag="ONT64_000141"
     CDS             5443..5898
                     /locus_tag="ONT64_000141"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HCR2301586.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            5895..6653
                     /locus_tag="ONT64_000142"
     CDS             5895..6653
                     /locus_tag="ONT64_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR2301587.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6739..7479)
                     /locus_tag="ONT64_000143"
     CDS             complement(6739..7479)
                     /locus_tag="ONT64_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2301588.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7540..8793
                     /locus_tag="ONT64_000144"
     CDS             7540..8793
                     /locus_tag="ONT64_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR2301589.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9115..9342
                     /locus_tag="ONT64_000145"
     CDS             9115..9342
                     /locus_tag="ONT64_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR2301590.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9465..9791
                     /locus_tag="ONT64_000146"
     CDS             9465..9791
                     /locus_tag="ONT64_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCR2301591.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9859..10572
                     /locus_tag="ONT64_000147"
     CDS             9859..10572
                     /locus_tag="ONT64_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCR2301592.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10882..11733
                     /locus_tag="ONT64_000148"
     CDS             10882..11733
                     /locus_tag="ONT64_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2301593.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11854..13719
                     /locus_tag="ONT64_000149"
     CDS             11854..13719
                     /locus_tag="ONT64_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2301594.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13767..15176
                     /locus_tag="ONT64_000150"
     CDS             13767..15176
                     /locus_tag="ONT64_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCR2301595.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            15284..15985
                     /locus_tag="ONT64_000151"
     CDS             15284..15985
                     /locus_tag="ONT64_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2301596.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16144..16623
                     /locus_tag="ONT64_000152"
                     /pseudo
     CDS             16144..16623
                     /locus_tag="ONT64_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            17471..18046
                     /locus_tag="ONT64_000153"
     CDS             17471..18046
                     /locus_tag="ONT64_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCR2301597.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            18046..18486
                     /locus_tag="ONT64_000154"
     CDS             18046..18486
                     /locus_tag="ONT64_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCR2301598.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            18502..19203
                     /locus_tag="ONT64_000155"
     CDS             18502..19203
                     /locus_tag="ONT64_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR2301599.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(19691..20116)
                     /locus_tag="ONT64_000156"
     CDS             complement(19691..20116)
                     /locus_tag="ONT64_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301600.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            20490..21461
                     /locus_tag="ONT64_000157"
     CDS             20490..21461
                     /locus_tag="ONT64_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2301601.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            21493..22890
                     /locus_tag="ONT64_000158"
     CDS             21493..22890
                     /locus_tag="ONT64_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2301602.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            23049..23672
                     /locus_tag="ONT64_000159"
     CDS             23049..23672
                     /locus_tag="ONT64_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301603.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            24421..26514
                     /locus_tag="ONT64_000160"
     CDS             24421..26514
                     /locus_tag="ONT64_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR2301604.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            26674..27540
                     /locus_tag="ONT64_000161"
     CDS             26674..27540
                     /locus_tag="ONT64_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR2301605.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(27562..27999)
                     /gene="ndk"
                     /locus_tag="ONT64_000162"
     CDS             complement(27562..27999)
                     /gene="ndk"
                     /locus_tag="ONT64_000162"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR2301606.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            28658..31336
                     /gene="mgtA"
                     /locus_tag="ONT64_000163"
     CDS             28658..31336
                     /gene="mgtA"
                     /locus_tag="ONT64_000163"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR2301607.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            31423..32094
                     /locus_tag="ONT64_000164"
     CDS             31423..32094
                     /locus_tag="ONT64_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR2301608.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     misc_binding    32171..32404
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    32447..32685
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            32741..33814
                     /locus_tag="ONT64_000165"
     CDS             32741..33814
                     /locus_tag="ONT64_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2301609.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            33816..34496
                     /locus_tag="ONT64_000166"
     CDS             33816..34496
                     /locus_tag="ONT64_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2301610.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            34513..35352
                     /locus_tag="ONT64_000167"
     CDS             34513..35352
                     /locus_tag="ONT64_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR2301611.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            35383..36606
                     /locus_tag="ONT64_000168"
     CDS             35383..36606
                     /locus_tag="ONT64_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCR2301612.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            37009..38427
                     /gene="nhaC"
                     /locus_tag="ONT64_000169"
     CDS             37009..38427
                     /gene="nhaC"
                     /locus_tag="ONT64_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCR2301613.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(38931..39374)
                     /locus_tag="ONT64_000170"
     CDS             complement(38931..39374)
                     /locus_tag="ONT64_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HCR2301614.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(39592..40932)
                     /locus_tag="ONT64_000171"
     CDS             complement(39592..40932)
                     /locus_tag="ONT64_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2301615.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            41201..42097
                     /locus_tag="ONT64_000172"
     CDS             41201..42097
                     /locus_tag="ONT64_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCR2301616.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(42247..43188)
                     /locus_tag="ONT64_000173"
     CDS             complement(42247..43188)
                     /locus_tag="ONT64_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR2301617.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            43314..44435
                     /locus_tag="ONT64_000174"
     CDS             43314..44435
                     /locus_tag="ONT64_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR2301618.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            44693..46195
                     /gene="eat(A)"
                     /locus_tag="ONT64_000175"
     CDS             44693..46195
                     /gene="eat(A)"
                     /locus_tag="ONT64_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCR2301619.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            46223..46789
                     /locus_tag="ONT64_000176"
     CDS             46223..46789
                     /locus_tag="ONT64_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR2301620.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            47000..48061
                     /locus_tag="ONT64_000177"
     CDS             47000..48061
                     /locus_tag="ONT64_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR2301621.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            48064..48960
                     /gene="murQ"
                     /locus_tag="ONT64_000178"
     CDS             48064..48960
                     /gene="murQ"
                     /locus_tag="ONT64_000178"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR2301622.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            48974..50956
                     /locus_tag="ONT64_000179"
     CDS             48974..50956
                     /locus_tag="ONT64_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2301623.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(51021..51467)
                     /gene="nrdI"
                     /locus_tag="ONT64_000180"
     CDS             complement(51021..51467)
                     /gene="nrdI"
                     /locus_tag="ONT64_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR2301624.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            51678..53102
                     /locus_tag="ONT64_000181"
     CDS             51678..53102
                     /locus_tag="ONT64_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2301625.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(53283..54056)
                     /locus_tag="ONT64_000182"
     CDS             complement(53283..54056)
                     /locus_tag="ONT64_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR2301626.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(54053..54958)
                     /locus_tag="ONT64_000183"
     CDS             complement(54053..54958)
                     /locus_tag="ONT64_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2301627.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(55112..55432)
                     /locus_tag="ONT64_000184"
     CDS             complement(55112..55432)
                     /locus_tag="ONT64_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HCR2301628.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            55906..56592
                     /locus_tag="ONT64_000185"
     CDS             55906..56592
                     /locus_tag="ONT64_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HCR2301629.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGIASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            56594..57538
                     /locus_tag="ONT64_000186"
     CDS             56594..57538
                     /locus_tag="ONT64_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HCR2301630.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            57737..58381
                     /gene="pcp"
                     /locus_tag="ONT64_000187"
     CDS             57737..58381
                     /gene="pcp"
                     /locus_tag="ONT64_000187"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCR2301631.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            58460..59737
                     /locus_tag="ONT64_000188"
     CDS             58460..59737
                     /locus_tag="ONT64_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301632.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(59921..60670)
                     /locus_tag="ONT64_000189"
     CDS             complement(59921..60670)
                     /locus_tag="ONT64_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HCR2301633.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            61000..61380
                     /locus_tag="ONT64_000190"
     CDS             61000..61380
                     /locus_tag="ONT64_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2301634.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            61476..61985
                     /locus_tag="ONT64_000191"
     CDS             61476..61985
                     /locus_tag="ONT64_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301635.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            62062..62241
                     /locus_tag="ONT64_000192"
     CDS             62062..62241
                     /locus_tag="ONT64_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301636.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            62266..62859
                     /locus_tag="ONT64_000193"
     CDS             62266..62859
                     /locus_tag="ONT64_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2301637.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(62906..63421)
                     /locus_tag="ONT64_000194"
     CDS             complement(62906..63421)
                     /locus_tag="ONT64_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301638.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(63447..64316)
                     /locus_tag="ONT64_000195"
     CDS             complement(63447..64316)
                     /locus_tag="ONT64_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2301639.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            64442..65122
                     /locus_tag="ONT64_000196"
     CDS             64442..65122
                     /locus_tag="ONT64_000196"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR2301640.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            65275..66255
                     /gene="pta"
                     /locus_tag="ONT64_000197"
     CDS             65275..66255
                     /gene="pta"
                     /locus_tag="ONT64_000197"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR2301641.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            66378..66851
                     /gene="tsaE"
                     /locus_tag="ONT64_000198"
     CDS             66378..66851
                     /gene="tsaE"
                     /locus_tag="ONT64_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR2301642.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            66844..67368
                     /locus_tag="ONT64_000199"
     CDS             66844..67368
                     /locus_tag="ONT64_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2301643.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(67858..68391)
                     /locus_tag="ONT64_000200"
     CDS             complement(67858..68391)
                     /locus_tag="ONT64_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCR2301644.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(69255..70007)
                     /gene="xth"
                     /locus_tag="ONT64_000201"
     CDS             complement(69255..70007)
                     /gene="xth"
                     /locus_tag="ONT64_000201"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR2301645.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            70120..71040
                     /locus_tag="ONT64_000202"
     CDS             70120..71040
                     /locus_tag="ONT64_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2301646.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            71113..72048
                     /gene="murB"
                     /locus_tag="ONT64_000203"
     CDS             71113..72048
                     /gene="murB"
                     /locus_tag="ONT64_000203"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR2301647.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            72346..73824
                     /locus_tag="ONT64_000204"
     CDS             72346..73824
                     /locus_tag="ONT64_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2301648.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            74101..76161
                     /locus_tag="ONT64_000205"
     CDS             74101..76161
                     /locus_tag="ONT64_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HCR2301649.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            76244..76813
                     /locus_tag="ONT64_000206"
     CDS             76244..76813
                     /locus_tag="ONT64_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2301650.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(76988..77794)
                     /locus_tag="ONT64_000207"
     CDS             complement(76988..77794)
                     /locus_tag="ONT64_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301651.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            77932..79446
                     /locus_tag="ONT64_000208"
     CDS             77932..79446
                     /locus_tag="ONT64_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HCR2301652.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(79849..80832)
                     /locus_tag="ONT64_000209"
     CDS             complement(79849..80832)
                     /locus_tag="ONT64_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HCR2301653.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(80837..81484)
                     /locus_tag="ONT64_000210"
     CDS             complement(80837..81484)
                     /locus_tag="ONT64_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2301654.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(81569..82201)
                     /locus_tag="ONT64_000211"
     CDS             complement(81569..82201)
                     /locus_tag="ONT64_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR2301655.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(82217..82768)
                     /gene="coaC"
                     /locus_tag="ONT64_000212"
     CDS             complement(82217..82768)
                     /gene="coaC"
                     /locus_tag="ONT64_000212"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HCR2301656.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(82765..83502)
                     /locus_tag="ONT64_000213"
     CDS             complement(82765..83502)
                     /locus_tag="ONT64_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HCR2301657.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(83499..84488)
                     /locus_tag="ONT64_000214"
     CDS             complement(83499..84488)
                     /locus_tag="ONT64_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR2301658.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            84746..85288
                     /locus_tag="ONT64_000215"
     CDS             84746..85288
                     /locus_tag="ONT64_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2301659.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            85305..86390
                     /locus_tag="ONT64_000216"
     CDS             85305..86390
                     /locus_tag="ONT64_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2301660.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            86402..87208
                     /locus_tag="ONT64_000217"
     CDS             86402..87208
                     /locus_tag="ONT64_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2301661.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            87209..88036
                     /locus_tag="ONT64_000218"
     CDS             87209..88036
                     /locus_tag="ONT64_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2301662.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            88033..89106
                     /locus_tag="ONT64_000219"
     CDS             88033..89106
                     /locus_tag="ONT64_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2301663.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            89534..92230
                     /locus_tag="ONT64_000220"
     CDS             89534..92230
                     /locus_tag="ONT64_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2301664.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            92307..92795
                     /locus_tag="ONT64_000221"
     CDS             92307..92795
                     /locus_tag="ONT64_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2301665.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            92807..93301
                     /locus_tag="ONT64_000222"
     CDS             92807..93301
                     /locus_tag="ONT64_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2301666.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            93322..94131
                     /locus_tag="ONT64_000223"
     CDS             93322..94131
                     /locus_tag="ONT64_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2301667.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            94128..94949
                     /locus_tag="ONT64_000224"
     CDS             94128..94949
                     /locus_tag="ONT64_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2301668.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            95000..97099
                     /locus_tag="ONT64_000225"
     CDS             95000..97099
                     /locus_tag="ONT64_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HCR2301669.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            97096..98214
                     /locus_tag="ONT64_000226"
     CDS             97096..98214
                     /locus_tag="ONT64_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HCR2301670.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            98253..99131
                     /locus_tag="ONT64_000227"
     CDS             98253..99131
                     /locus_tag="ONT64_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2301671.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            99322..99474
                     /locus_tag="ONT64_000228"
     CDS             99322..99474
                     /locus_tag="ONT64_000228"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCR2301672.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVLVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT        32588 a        17205 c        20867 g        29002 t
ORIGIN      
        1 ataaagaaaa caatatcaaa aaatgcacgt agaagtagat aaatggagtg gttttgcaaa
       61 tttcttttat ttctagaagt tattcacgta tacggatttg tttttctcac aaataaaaag
      121 gcaggaagta caatggatca tttgtatata aaatatattc atcaattttc cgttttggtg
      181 ttaggcacac taattatagc ggggattgtc gcatttatta ttcaaggccg tgacatatta
      241 acagtacatc aagagttttt cagttatgca tttggtattc ctctgatcgc tataaggtct
      301 gataacaaaa agtggaagaa acgctattac attctcttgc tatttttgtg tttttatctg
      361 ccacctaaga tttttacttt attaggtata caatgcctat cattttcttt tatttcctat
      421 atcattgttt taattttcct cagtttcata gtatggatgt tttatcttgt caaaaaataa
      481 atgaatgttt catgaacagt acttcatcac agcctaattt ttttctttat gctatcgtta
      541 tattaaagaa atcaatcaga agggaaagaa agtgatgaaa aaagttttat tagtggtaaa
      601 ccccagttca ggcggtgaac aagcaaagga atttgagcaa ttagcgatag caaaattgga
      661 atcggtgttt gatgaagtgg tcgtcttgca tacaaaaaaa gcaggggatg caaaaaactt
      721 tactcgcgaa gcggctacgg aagggtatca tagtgtgttt gtcatgggcg gagatggaac
      781 agtcaacgaa ggaatcagtg gaatcgctga acaagaacat cggccaaatt tcggattttt
      841 cccattgggt acggtgaatg acttggctcg agcattaggg ataccgctag aacctgagga
      901 agcgatcaat catttttcga ttgaatcagt caagccattg gatattggaa aaataaatga
      961 cgactatttt atgaatgtcg tcgcaattgg ctctatccct gaatcaatca atgatgtaga
     1021 tgcggagaag aagacgaaat ttggaaaact agcttatttc atgtcaggaa tcaaacagtt
     1081 agcttccaca caatcctact cgtttcacgt agaagttgat ggaaagaagg aagaaattga
     1141 gagcagtacc ttgctgatag gactaactaa ctcagtcggc ggttttgaaa cattacttcc
     1201 aaatgcaaaa gtggatgatg gtaagctcca tttggtttat ttaaaagatt cttctttgat
     1261 cgatacgctt aaaacattgc cagacctatt aaaaggagta gatgaatcta cgaaaaatct
     1321 agcctacctt acttgtgagc aattagttgt gtcattagct gatgaggaag aattgacgac
     1381 gaatgttgat ggagatgaag gggataaatt acctgtgaca atcaaagtat tgccctcaca
     1441 tttacaggtt tattgttaaa atggaggttg tgaccgagga gttggcggga gaacaccgct
     1501 tgttcgggat aagggactgt gacaagactt ttgtcacagc cttttttttg tacaaaaaag
     1561 aaatttacac ctaatagaaa aatttttcca taaaaataca tattttaaat atgaaagtac
     1621 aattggaaat agattaaaac gataaaataa aattgtcaaa acgtcagaga ggatggtgga
     1681 gaatgaatca atgtgtaaaa aagctgattg cgtcgatttt gtttgtcttc cctctcttga
     1741 tggtgacatc cgtttttgcc gatgtagaga aaacaaacga atcaacgcaa tcagagaatc
     1801 aaagttttga caagactact ttttcagaag aatcgattac ttctgaaact acagaatctc
     1861 aaagtgaggc ttctactaat aaaaggcaag aagaaaataa gacaagtgag acctcagaag
     1921 tagctgaaca aacgtctgat agcagtgaag cggcactggc aagagctgtt tcagcggatg
     1981 ggtacagcga agctgccaca acagaagaac tggcgcagct tctcgcagac gaatcagtaa
     2041 ctaagatccg actgattcag cctttgactt tagacaggga attagagatc aaacgagata
     2101 tcgttattga ttttggtgga ttcgctcaca atttcggcac acaccatatt tatatcaatg
     2161 agatacctca gtcgatcgag ttccagaatt ttaaaggaac agctgcacat ccaggtggat
     2221 tgacaccaaa tatcgatgga aatgccatta tcattgctta tatgaccgat ttttggggaa
     2281 gcagatatca ttttacggga gaaataaaat ttaccgggac acttgatttg tatgatgggt
     2341 ccaaattagg actgatttac gctcctcgtg caactgtcac attagatggt gtttccggtg
     2401 tgttggatgt ccaaccagtg aaggaaggac tgaatgccgc tccgggtaaa gcctattttt
     2461 gtagaagcta tcaattgaat gtgatcaatg gtagtcaact ttatggaccg tatctaggga
     2521 aattctacgg ttttctagat tcaggagatg agactggtag tggaaaaaca gcgccaggaa
     2581 tccatatctt aagcggttca aaggtctctc ttgattatga tcgggcagat ggcgttagcg
     2641 atgaaggaga ggcagtcgat acgctacctg gtaatgtcat gtttgaagtc agtggttcgg
     2701 gaagcgaatt ttcggtaaat acaaagatca atattaccaa tgacaacaat cgcggaatca
     2761 tccaaatgag aggttcagga tcgaaagtca ctgtatcgaa tggtgggaaa atcaccatca
     2821 atacagcaac tacgggcgga tttcgcttgc aaggagattc tagtcaaatc catgtatcaa
     2881 gtggtggaca aatctcggtc aaaatggctg gagatgacga tcaacccggg aacaatggga
     2941 tgcgttttgt aggaaaaggc ctttctttga tcgtagaagg agccggatca gttatcgaca
     3001 tcgataagca gagtgggcgt tccagcgcag ttcgttttga gaatggtacg caaaagctga
     3061 cagtaaccaa tggaggctca ttgagggtaa gaaatacagg tgatggcaaa tcttacgtga
     3121 acagactcaa tcaaggaaat caagcgatcc aattttatga cgcttctggt tttctagaaa
     3181 gtccaggttc agctgatttt acagtcactg gagaaaaatc aaatatcgat atacgcgcag
     3241 attccggagc aactgtagat acaactggag atattgattt aaattttacg gcaggtgaaa
     3301 aaacttattt agtcatggtc gggaaaacag gggaagataa gaccggcatc atcagtgggg
     3361 atgtgttgaa tgtggactta gaatctccta cctattttga tttccaaaac aaacggattg
     3421 gaagcgagac caatggcata ggcggctggg tattccaagg aaatggagat tcttctttaa
     3481 agatcgccca ttcagaaatc gctttatggc gaaaaacagg cgcaacattc gataactttg
     3541 aaaaagaccc ggattatttt tctgatatga cagacttatc gatcaatggg accgacttag
     3601 gcaatttcgt atcaagccct agtgcaggtt taacaaatga atttgctcag acaggtgccg
     3661 gaatgaaaaa ttataatcga atcagtgcca ataatcagct cccgatgatt gaatccttaa
     3721 gagtgccaac gaatgcagat aaaaaaatct atgggcatgt cgttgttccc gaaggtgtcg
     3781 aggcaaagcc aagagatgct tggacaggcg aagtagaagt gactttacgt attacttacg
     3841 cagatacgac gaaatcatct gtagaaatta ctgctctcac gcaaggacaa acagatgaaa
     3901 atgaaggggc aggatataac ccatggggag aaggcgaaca aggcggcttg ttcgaagttc
     3961 aagtacccaa tgatgagaga ttaaaaacgg aggatcaagt gcaagttgtt gctgctaaaa
     4021 aagtaagagg tggccaagaa gtgacagaac tggactctgc caaaacgaca gtcgatgtga
     4081 ttcctcctga acctgctaat ttaagtacca cattgatcaa tgcagcttct cgcacgatca
     4141 caggtacagg tgaagcagga gccttggttt cattgaaaaa aaatcaagct gttttgtcag
     4201 aaacaactat tgatgataca ggggaatttg ttctttctat cccagaaaat caactttttg
     4261 caggagatgt attagaaatt gtattaaacg accaagcagg agaagctact gctaagggtg
     4321 tgcagaacaa gccaagtacc aacgatgaaa ttgggaatca caatccaagt gatacgccag
     4381 ttgtttatca tgatgccccg ccttttgcac ctgctacgaa gatcgtagta gaaggtggtc
     4441 tctcatttct tgccccttcc aaactcgatt ttggtcagat caaagcaact ggtttgacac
     4501 aagaagcatt tggccaaatg gatatacagg aaaaattatg gatttatgat cagcgagaaa
     4561 cgaaaaatcc gtggatctta acgataaaat tatctcagcc tttcacaaat gatgcaggat
     4621 tttctatgca ggatgtccta tatttcaaga aaggagagga gtatcaatta gtgaatgaag
     4681 aagaactgga aattgtcaga ggacaaaatt caacgaatgt gcaagcagat tattcagaat
     4741 atttaaataa cggtcgggcg tttaaacttg tcttaaccaa agaaaatcag atagctggta
     4801 attactcagc agagataaca tggacattag cagatgcgcc gacagaataa agatcaagga
     4861 ggatgattga tgaatcgaat atggaataaa agtcatcgaa acaactgctc tttacttatg
     4921 atccttctcc ttggattcat cctttggcag acacctgtcc atgcctccga attcaatttt
     4981 gcggtagatt ttgaattacc ggctaatcag attgataaga acaaaacata tataaatgta
     5041 aggatggagc ctgggaaaac acaggaaatc ccctacacgg tctttaaccg aacagataaa
     5101 tcagtaacag ttgagacatt gatccactcc gctacgacga attcaaacgg tgtgatcgaa
     5161 tatggagaat caaaaagtaa atttgatgaa acattaagtt atgatatgaa aaacttagca
     5221 aaaacagtgg gagaaataac tgttcctcct caaagtgcgg tcactgagac actttcactg
     5281 aaagcgccag aaaaagagct ggctggtgtg gtcgcaggag ggatcacgtt tcaagaaaaa
     5341 aaagagaacg tttctaacaa aagtacagtt ggtgtcgtca atcagttttc tcaagtgaaa
     5401 gcaatcttga tttgaaatag cgaaaaagag tcttccccag atttgaaatt aaaaaaagct
     5461 tatgcggatc aaagcgatgg aagaaatgtg attaaggcga atcttcaaaa tccaaatgct
     5521 tcttatatgt tcggcttgac tacatcggtt gaaatctaca aaaaagaaac aaaagagcct
     5581 tacctttcaa taaaaaaaga aaaaatagat gtagcaccca atacaaattt tgatttactt
     5641 gtcccattag aaggaaaaaa attagctccg ggacattatc aagcggttgt gaagggtgct
     5701 tggaaagaaa aacgctggaa tctccatacc gattttgaaa taactgatga tgcagcggac
     5761 caattgaatg aaaaggatat tgatttaaaa aatcagccag aaacatttga ctggcgatgg
     5821 ctgatacttg ttggagtcat tcttattggc ttatttgctg gattatttat tttaaaagaa
     5881 agaaggaaaa agaaatgaaa aaaacaattg cattaagtac cgttctagct ggaagtctat
     5941 tattatgtac acaaagtgtt tttgctgaga ataagagctc agccactaca gaaggaacga
     6001 tttctgtgac caaaccagat gctgcaaatc ctgaagacgt tgttccagtt cctcctgttg
     6061 atccaaatga tcctgatact ccttttacac ctgacgaacc aaatcaggga acatccggat
     6121 tactgacgat tgaccaagct ccagatcttg attttggaac aatcaagtta ggtacagcaa
     6181 aaacagtata tgctgcatta caaagcggaa cggacagtac tggagcacaa acacaagtac
     6241 agaatttcgt acaagtaacg gataaaagcg gaaattatgc cggttggaaa ttatcagtca
     6301 aacgaacaga atttattggt acaacatctg atacgaataa actaactggt tcaaaggtga
     6361 aattcaaaaa cgccgtgatt cgaaatagcg caactaatag cagtggaatg ccatctaccg
     6421 tttctggaac tggcgctgaa ggaatcgaaa tcccagcaga tcaggcagta gatctagtaa
     6481 ctgcaggagc aaatgaaggg attggcacat ggatttattc attaggagag aatgtggaac
     6541 aagggagaca atcagtggaa ttatttgttc ctgtatccaa ttatgctgta gacaactacg
     6601 tttcaacatt cacatggtcg ttaacagatg ctcctacaga agaggaagcg taaaatcata
     6661 tcaacgattt cagggagtgg agccgattca aggcccactc cctgaaatct gctatgctta
     6721 cgtctcagct cttattcatc aactcgaagc agttttttgg aagtcatgga aacgttcctt
     6781 tgtcttttca aagatctttc gaccaacgag taaggagtca ccgttgaaaa attctaacac
     6841 ataatgagca gaatcaattt tctgcaattg cgataagttg atacagtaag attttaaaag
     6901 taaaaagtaa gcatactttt ctaactctgt cttcaattgt tttaaagaag tattcactaa
     6961 tttatcttct gtagttgttt tgaccataac ttggtttttc aaacctttga tactttcaat
     7021 atataaaatt tcccgaggat acaggacgag acttccacct ttttgatcaa taaagatttt
     7081 atcgacattt acgatctgct gttctttcca attgatctgt tctagcactt gacgaatttg
     7141 ttcagataaa ttcctgtctt ttctgatata tccaatcgga aggattttcc gattgatgat
     7201 ctctactgca aaattcgtat aagcggtgaa aaaaacaata ttactatgtg catttttttt
     7261 acgtatttgt tcggcgacat caatacctgt gagtgattgc ttcaagtcga tatcgataaa
     7321 gaaaacatcg tgattatcaa tagtgcccaa ctggatcaac tgattgattt gagagataga
     7381 agtgagctgc acgatattct cgatatgatg attgaccaat tctctgatga tccattcgct
     7441 gtgaaactgg ttatcttcta tgataaaata ctttttcata tgcaggctcc tattaaaaaa
     7501 atatgtgtac attttgaaac aaaacaatgg aggaataaga tggtcgcaac agtgacactt
     7561 tatttgacct ttactttgat tttagattta ggtatctatt tattcttttc gaaacaactg
     7621 ctagtaaaaa acagctttgg ttgtatagct ttgatggtgg tgacactttt acttcctatc
     7681 cctaaagtga gctttcctta cctagttggc ttattattga tgtttctttt ttattttact
     7741 acgaaaaacc tgtttatcag ctgtttgatc agtaatcttt tttttgctat cactggactc
     7801 agcttttttc tggtttttga tctgatggat tatttaggaa agagacatct tggaacagca
     7861 agtttttttg cactacttat cgcatttatt ttctatctat tagtaaaaag tatagacaaa
     7921 cagacgagat ttctcaactt gctttttact tatccaactg aacactattt tggaggtatt
     7981 tgtattttat taagctgggg cgtgatttac ttggtattgg gaagtgttca cttccgctca
     8041 tctaattatc tgcttctatc tcttttgtcg atagtcatca gttttttttc tttgtttttt
     8101 tccattatgt tgatcagttt ccaagtaaaa gaaagacaac atcaggaatc gcttcaactg
     8161 tatcaaataa atgaagaata ttaccatcat ctagaggaat tcaaacatga ttataaaagt
     8221 ctactgtttt cattaaaaat tactcttcag gaaaataaaa aagaaacgtt ggaattttta
     8281 gaaatattag aagatcattc tgcagctatt tttgaaaaac cgcaaataca acagctaaca
     8341 aatattgtat ccccagctgt caggggtgtc tttttaaaat ttctagaaaa agcagaatcg
     8401 gagaaaatcc cgatccaagt agttattcct gtcgaagtga aaacaatccc gattgatctg
     8461 gttgttttta ttcgctgttt atctattttt atttctaacg cattcgatca tcgtgtagcc
     8521 gatcagagtc cgattacgat caatctgctg gaagaaaaag agggatcacg ttttagtata
     8581 gcgaacaagg caaatccaac gaatttgacg ctatcggaga tggtcaaaag aggaaaaacc
     8641 tctaaaaaaa atggcgggtt aggtctatac agtgcgaaaa aaatgttgga tgtttacgaa
     8701 aatgcagaac taaaaatcga attttcaaaa cagaatcact atttttttgt agaaatgtat
     8761 ctggcatcta gaaagagcaa atcagcctac tgacttactg tcaaaggata agtttgagga
     8821 aagtctacac ggagatagag acaacaacac agcatattca aaagaaacag aagcgtgatg
     8881 accgttgtca cccttctgtt tcttttgcta tgtagttaaa caaagtagtt gacacttccg
     8941 ctacttagta ttagtacgaa agtatacttc atgatactaa gtagataaga agtgaaaagc
     9001 ggaacccacg atcgtcaaaa tgctaactgc tttgcttaca ccagataaag ggaagagcaa
     9061 tatcgcttat tttaggcgtg gagaagcaag caaatcccta cgatcacaaa aaaaatgctg
     9121 acccagcagt taagagagtt agaacaagat gaaagtatcc atcgaaaagc ctataaccaa
     9181 gtcccgccta aagtagagta ctcgctcacc gaagaaggaa aatcattaag agagattcta
     9241 gtagcgttgt ctgtttggga agaagaacgt atcgaaaaac aaaaagcctc aagggaagaa
     9301 gacacgctac tgcatacaca tggtggctac ctaaattatt gattttctat gacatttatt
     9361 ttaaataaaa gaaaacagga gtttttttaa aagcatcatc tattttttat gtaaaaaaat
     9421 ggtatgatta atcaagtaag aagtgattgg aagaaggcaa gcaaatgaat agtatggaaa
     9481 aagtcaacca gtttagagat gaacgtaatt ggcgacaatt tcataacgaa aaagatttgg
     9541 ctatctctat ttctttggaa gcatctgaat tgttagaact ttttcaatgg aaaacgccgg
     9601 aagaagtaaa agaaacacaa ttagaacgaa tcaaagaaga actggcagat gtattgatct
     9661 attcatacat gatggcagac aaccttcacc tggatctaga tgagatcatt gaagaaaaat
     9721 tgatcaaaaa taatttgaaa tatcctgtag aaaaaagcgc aggtgtacga aaaaaataca
     9781 cagagttgta gaaatgtgtc attcggctgt tggaaagagc aaaagaaaaa cagtaaaatg
     9841 agtctggagg gagagaaaat gaaaaaaaaa ggaaaagcaa aaagatggct agtcaatatc
     9901 ctcttgttcc tcttgttatt agtaggacta gctttgattt tcaatgaaca gatcaaggat
     9961 tattttgtca gagagacagg agataaatat gctatagcga atgtaacaaa agaagatctg
    10021 aaaaagaata acgataaaga tgtcagtttt gactttgatg cggtagaacc aatgacgaca
    10081 gaaggggtca tgaggtcgca aatgagagga acagatctac cagtgattgc tagtatcgca
    10141 gtcccttcag tttcaatcaa tttaccggtt ttcaaaggct tggataatac gtcactactt
    10201 tacggagcag gaacattatc tccagatcaa gaaatgggaa aaggaaatta tgccttagca
    10261 agccatcgtg caacgaaccc tgaattgctg tttacaccgc tagaaaattt agagatgggt
    10321 gccaagatct atttgacaga tttagaaaat gtgtatacat acaaaacatt ttttaaagaa
    10381 aaagttgctc ctacagatac ccaattactg aatgaagtcg agggaaaaga aattgtcaca
    10441 ctaattactt gtggcgacat ggacgccgtc acgcgactag ttgtacaagg agaactggaa
    10501 agtgttacat cgataaaaga tgcgacagat gatatgagat ctgctttcaa tctagagaca
    10561 aaaacatttt aggatactgg gacataagtc gttacagatt aaaaacagcc aaaggatatg
    10621 aatcgataag gaaaaaccaa tttgctgaaa aaaagagaaa cgcaaagagg tgcatctctg
    10681 atttggttgt tttctagata gacttgtgtt ccagtttttt tattttttat atgaaaaggc
    10741 ttgcataaat aaaaattcga tggtatgatg attttgaata agaaatggat tgttactgta
    10801 cggcaggcaa aaccagaatt cagctaagaa acagtaggcg aattctaggt ttttttattt
    10861 ttcttgagag ggcgaagcaa aatgaaaatt gataaaattt tgaataacaa tgtcgtcatt
    10921 tcgaaaaatg gattcgggga agaagtcgtt tgtatgggaa aaggactagc ttttcaaaaa
    10981 aagattgggg acgagatttc gccagaagcg gtccaaaaag aatttgtttt aagagattct
    11041 tttgctaaaa accaattcca gcagctgatg gctgatattc cggcagagga gatcgaatgg
    11101 gtcaaacaag tagtcgaact ggctgaagat aaactaaaag ttgaattttc gcctaatatt
    11161 tatctgacat tgaccgatca tcttcattat gcgatcacac gagcaaaaga aaatatccaa
    11221 ctgcctaatc cattattatt tgaaacaaaa aaattttatc cgaaagaata tcaagcagcg
    11281 aaagaggctc ttttgttcgt gaaacaaaag acgggtgtgg aactttctga ggatgaagcc
    11341 ggttttattg cctttcattt tgtgaatagc caacaaggaa acgaagacat gcaagtgacg
    11401 atgaccgcta ccacaatggt aagagatata ctgagtatca tcagtaaatt cttcggcatc
    11461 gtgttcgatg aagaatcttt gaattatcag cgtatcatta ctcatctgca gttttttgcc
    11521 cagcgttatc tcaaagggga aggatctgac gagcaagatg aatttctcta cgcattagtt
    11581 caaggaaagt atccaaaagc gtttcattgt gtcgaacgga tcaatgagta tcttcgacaa
    11641 acgcaaaaac aggaaatggg gatcgcagag cagatttatt taacgattca tattcaaaga
    11701 gtagtaagtg agaaaaaaag catccaatca taaggattgt tactgtatgg caggcaaaac
    11761 ctggattgaa aaaataggtt cacgaatgtt tgtgactgtt tttttcaatc tgggttttta
    11821 ttttttaaat aaaaaagaaa gaaggaaaaa acaatggata atcaagcagt tggtcgtcgt
    11881 gtatgggaag cggtaggagg acaaaagaat gtcaaaagtt tagtacattg tgctacgcgg
    11941 ttgcgattca gattaaaaga tgaatcttta gcagatacgc aaaagttaaa agaagacccg
    12001 gacgtgatcc aagtcgtaca aagtggcggt cagtatcaag tagtgattgg cagtaatgta
    12061 gcagatgttt atcaatcgat cgtggatgag gaaggtcttg cagtttcgga ggatggaaaa
    12121 gaagagtcca aaaatatttt gaatcgtctg atcgatatta tctctagtat atttacacct
    12181 tttctagggg cgatggcagc cgccggggta ttgaaaggat tcttatcatt ggcaacggtg
    12241 atgggctggc tgacagctga tagcggtact tatcaaatat tgtttgctgc agcagatggt
    12301 gtctttacct tcttacctgt catgcttgct tttacagcag ctaaaaagtt caaagccaac
    12361 cagtttttag cagtagcaat tgctatggca cttgtgtatc cagctatcac agcagttgct
    12421 ggtgcgggag aagctatcag tttctttggg atcccagtga ttctttcacc atctggttat
    12481 acttcttctg tcataccaat cattctagcc gtatgggttc aaagtaaatt agagccattc
    12541 gtaaaaaaag tgattccgca atttctgcaa atgattttag ttccgcttgt cgttttagta
    12601 gtgatggttc cattaacatt tcttgcttta ggaccaatcg ggacggttgc cggtaatgct
    12661 ttaggcggtc tcttcaactc gatttatgga tttagtccaa tcgtagcagg tctaatcatg
    12721 ggaagtttat ggcaagtatt cgtgatgttc ggcatgcatt ggggattcgt accgatcatg
    12781 tttttgaata tcgaacaata tgggtttgat gtattgatgc caatgcttct tccagccatc
    12841 ttggcacaag gtggggctgc tttagctgta gcattgcgaa caaaagatac gaaacttcga
    12901 gcattaggga tttcttctac agtgacttcg ttatttggaa tcactgaacc aactgtgtat
    12961 ggtgtgacat tacctttgaa gaaaccattc attgccgctt gtatttctgg aggtattggt
    13021 ggagctatta tcggattttc cggagtaaaa gctttctcca gcagtttggt cagcttattg
    13081 acgattccga ctttcattaa tacggtggat ggtgtagagt caaatgtgac agtagcagta
    13141 atagctacag gaattgcttt tgttcttgca ttcgtaggta cattgatttt aggatttgac
    13201 gaacaaacac aagacaatca attagaaaac aaacatgcga atgcaggaaa accgattact
    13261 tcagcacggc ataccttaaa aagtccttta acgggtaaag tactgccatt gagtgaagta
    13321 ccagaccaag ttttttcttc tggcgtgatg ggaaaaggta tcgctattga tccagaagta
    13381 ggagaactag ttgcacctgc agacggagag atcacgacta tttttccaac tggacatgct
    13441 gtagggatca caacgactga cggagcggag attttgattc atatcgggat ggatacagta
    13501 gaattaaatg ggaatggctt tgagatctta gtcaaacaag gggatctcgt caaagcagga
    13561 gatttattga ttcgttttga catcgaagca attagagcag ctggctatag cgtaatcact
    13621 ccagttgtca ttacgaatac tgatgcattt gccgatatac ttgaattaga ccaaaaagag
    13681 attattgcaa acgaagatgt tctggcaatt gtaaaataag gaggaaatca ggatgcaatc
    13741 acgtttttca gaaaaatttt tatggggtgg agcaactgct gccaaatcaa ttagaagggg
    13801 cttatgatcg agacggaaaa ggactatccg ttgcagatgc tatgcctgga ggtaagcagc
    13861 gctttgcgat tatcggcagt gaagaatttg actggacgat tgatcaagaa aaatatattt
    13921 acccgaatca tcgaggaatc gatcattacg atcgtttcaa agaagatctt gccttgtttg
    13981 cggaaatggg attcaaatgt tatcgttttt cgattgcctg gacacgaatt tttccaaatg
    14041 gagacgaagg tactcctaac gaagcgggat tagaatttta tgatcagctg attgatgagt
    14101 gcttaaaata tgatattgaa cctgtgatca cgatttctca ttacgaaatg cctcttcatt
    14161 tggcaaaaga atacggtggc tggaaaaatc gaaaactgat cgacttctat gaacgatttg
    14221 ctcagacagt attagagaga tacagcagta aagtcaaata ttggatgact ttcaatgaaa
    14281 tcaattcggc ctttcacttt ccagctttaa gtcaaggatt agtaaaaagc aatggagcag
    14341 gagagtacca aaatattttc caagcatggc ataatcaatt cgttgcaagt agcaaagccg
    14401 taaaaatcgg ccacgaattg cgttcggata tccaaatcgg ctgcatgatt atctatgcaa
    14461 caacttacag catcgatgcc aatccagtca atcaggcagc aacaatgatc caaaaccaag
    14521 aattcaattt tttctgcacg gatgtccaag tacgtggaga ataccctgca tacacagcgc
    14581 gtacccataa aaaatatgga gtcgatccgg aaaaattaga gcaaacggaa aaagacttcc
    14641 agttgctaaa agaatatcca gtggactaca tcggatttag ttattatatg tctacggcaa
    14701 tcaatgagac tgatccacaa gcagcaactt cagaagggaa cttgctggga ggagtaaaaa
    14761 atccgtttct tgaggcaagt gaatggggct ggcagatcga tccagaaggg ctgagaatcg
    14821 cattgaatga attatacaat cgttaccaga aaccattatt cattgttgaa aacggtcttg
    14881 gagccattga tcatatggaa gaggatggca cgattattga tgattatcgg atcgattatt
    14941 tacgtcgtca tatcgaagcg atggctaatg cagtagcaga cggtgtagat ttgatgggct
    15001 atacgccttg gggatgtatc gatcttgtca gtgcatcaac aggagaaatg agtaaacgtt
    15061 acggctttat ctatgtggat ctagacgatg aaggaaatgg gacactgaat cgatcaaaga
    15121 aaaaatcttt tgattggtat aaagaagtaa ttgcctcaaa tggcgtaaac ttgtaattaa
    15181 agcagctgaa tgaaaaagaa cacacttctc acatgtgatt tgtgttcttt ttttattttt
    15241 tatgtgctat tcttttagta atagtaaagc aaagggtgac gttatgaaaa ggacacgtgt
    15301 actatacttg gaagtcgcag ataaaattaa agaagatatt ttctcaggaa aatatccagt
    15361 cggttcgatg ttgccgacag aaagtgaatt agaagaactg tttcaagtca gcaaaatcac
    15421 tgtccgaaag gcaatcgaac tgctggcatc agatgaatat gtcgcaaaga aaagtggtcg
    15481 tgggacgacg gttctaagca atcgtcctta caatcgatta tccaaagcgg caagctttac
    15541 gcaaatcttg gagaactccc gatatgaagt ccggaaagaa acgctggatt tgcaattggt
    15601 ccatataaag aaggaagatc ctttgttttc ctttttcggt gagcaagctg tttgttttaa
    15661 gcgcttatat tatttgaaca atcagcctta tatttatttt gttcattatc ttccacaaga
    15721 aatgatgcaa ttcagcagag aagaatttgg caaagaatct ttgtatcgtt tgctgacgaa
    15781 aaaaggatat actattgact cattttctga tgatttctcg gcagtatttt taacagaaga
    15841 agaacaagga attctgaaaa cagacgcaca gatcggcatc aaacggatac ggaaatcgtc
    15901 tacgcaaatt ggggaaatga ttgaatattc ggaggcggtc taccatacgg agctgcatcc
    15961 ttaccatatt gattatgaaa cataaataaa aaaagaggtt gtgaaaaagc tgccctgcat
    16021 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttaggctta
    16081 ttaccgcaga tacacggctg tttctggatt accatttatt ctggataagg gggtgtggca
    16141 cgattttcag aaggaatcta tgaaccaatc actgtattga ttttagcttc gatggtaacc
    16201 ccattcggct tgacgatcgc ttatttctta gggaaaatca ttcgtaaaaa tatactcaat
    16261 cgtcaagaaa tcgatacatt gaagaccgct tttcctatgg ggatctgtca gatcacagag
    16321 ggatgtttcc ctatcgtatt gaacgacttg gcacgcaatg tcatagcaac gggagtcggt
    16381 gggacagtag gtggcggatt aagcatgttt tggggcgcag atagccgtat tccggcatcg
    16441 ggaatgtttg cagtcgctac gatgaccaga ccatgggcat tcattggtgc attgttagct
    16501 ggttcttttg tcacaggaat aaccatttta ttattgaaaa aaccagtaga tccgaatgca
    16561 gaaatcatcc aagaagaaaa agaggaagaa gacatatctt gggatgattt gacaattagt
    16621 taggagaggc agaatggatt acaagaaaaa agtacaatag atgtaccttg agacaggaat
    16681 ggtatgaaag aatcagagtt agaaagaatc gaatatacag attttggttt aggagcgatc
    16741 gaaactgtcc agagcatcgg caccctcctt gaaatgacga tgaaggaaaa atgagaacct
    16801 ttcgttgtcg aaagggtctg gtatacctta tgtcgaagga aaggaaacaa aaaaactttc
    16861 ctgtactatt ccaaaaagga aagaaagtta ttatacagta aaaaaagaaa ttattcttca
    16921 tgctagagat cagtacacgt tcgagcccaa tatcaaacac tagttccagg caggcgcaac
    16981 aggtgcggtt atcactgaat tttcttccag cagtgacgat tcaagtgata tttttacaaa
    17041 tccagctatt tatcgttaac aaaaagattt ttaggagaag ctgatgaaca aaaatgatca
    17101 aattttgatc gtttagttcg tcggcttttt tatttagaga aacatcattt atcagtgaaa
    17161 cataacacga gaacaaaatg attttttcgt taataaaaga aacaagtatt tgttgtttaa
    17221 aataatacac gttattggca tataggacga aatcccagta tcttctataa gtaagtcatt
    17281 tcatatcgat gaaaacacgt tatttatttt tgttttatct ataaaatggc tgaaaaatga
    17341 tttttttcta aatactatat atagtggcgt ctatcctata taccactaaa aaactacaaa
    17401 atctagtctt ttattttaaa aaaacacgtg tagaatgaag atagactttt gagttaggga
    17461 gtgttttttt atgaaactta ctgttttttg cggttcccgt tttggtaata aagaaagtta
    17521 caaatttatt gcgcaaacgt taggaaaata catggcacaa gaagaaatcg aactggttta
    17581 cggtggttca tactccggga ttatgggagt gattagtaaa acagtactgg aaaataacgg
    17641 aaaagtcaca ggtatctatc cgaatgggct ttttgaaact gaattgcctg gaaaagatgt
    17701 tactacattt attcctacag aaacgatcga tgaaagaaag gtattattat ttgaaaaggg
    17761 agatgctgtc ctcgttttcc ctggaggtct aggtacattg gaggaattct cacaacttct
    17821 ttcttggata gcaatcggtc tgacgcccga caagccaatc ggaatattga atattggcgg
    17881 ttattacagc ggcttgcaac aattattaga aacttttgca aaagaggatt ttatggataa
    17941 aaaatggttg gatcaagtaa tcttttccaa caatccattg gaactggtca atgaattacg
    18001 agcagtatct attaaaaatc agttattatt gaaggaggca aaataatgag tcaaatatat
    18061 ttagcaggac catttttttc agaggagcaa atcgatcgag taagtcgtat cgaaaaagcg
    18121 ttggaggaaa acaaaacagt cacaagtttc tactctccac gtcatcacca agaaagcaat
    18181 tatgaactat tcagcgctgg atgggcgcag gaagtatacg aaaaagatat ggaagaactg
    18241 acaaatgcag aatttgttgt tgctatcctt gactttgagc atcaaacaat cgatccagga
    18301 acggcttacg aattaggtgt ggcaactatg ttgaaaaaac cgatgatcat tgtacaagaa
    18361 gagacggtac ctacgaactt gatgattaca caaagtctcc atacgtattt aaaatcagac
    18421 caagctgtac gtgaatatga ttttgaaaca ttgcctgttg aaacttacgt aggcgagtac
    18481 ttatagaaga ggaagggttg tatgtcagtg attttgattt caggaacgat tggtgcaggt
    18541 aagagcagtt tgacggatat gcttgcaaaa gagattgatt ctaaaccatt ttatgaaaat
    18601 gtcgaagata acgaagtatt gccgctcttt tattcaaatc ctgagcagta cgctttttta
    18661 ttgcaaatct tctttttgaa caagcgattt ttagcaatga aaaatgcttt agtgaacgat
    18721 gacaatgtgc tagaccgatc catctatgaa gatagcctgt tgttccatct aaacgctgat
    18781 ttaggtcggg taacggatat cgaagtacag cagtacgata gcttattaga tacgatgcta
    18841 aacgagttag atgatgttgc tccaaaaaaa cggcctgatt tgatggtcca tatcaaagtt
    18901 tctttggaca cgatgctaga acgtatcaaa aaacgtgggc gtgactatga acagctagaa
    18961 agcgatgaga cgttatatac ctattatgaa acgttgaaca cccgctataa tcaatggtat
    19021 gaagattttg atgtatgtcc taaaatacaa gtggatgggg ataaattcga ttttgtagaa
    19081 aatccagaag atgcaaaaaa ggtgatccgt cagatccaag aaaaattagc tgaattggaa
    19141 ggaaagcctg ctggaaaata ttttacggat aaccagcatg caaaaggtca agaattaggc
    19201 taaaaataaa aaaccaaacg acgggaaaga aaaattacta cgtgatgtag aataagcctt
    19261 tctatcgttt ggttatttta tattcaaata tttgaaataa ataaatcttt actctttgat
    19321 ttgtaaactg aaatgatcca ctgtttgttt tactgatgaa gcttgatcaa aggaagaagc
    19381 tgagtttgaa gttcataaaa atcagccata gtgagttttg cttggatttg tttatcttct
    19441 tctacttttt acataatttt gaacaggata agattcaaat gttttaaatg aatctttgac
    19501 tagtttgtga cagttgtcgc atatgctcaa gcttccttgg tcattaaatt ctgtatggat
    19561 atttttgttg ttattacatg tacaataatc aggactatcg atcatccatt tcgtctcctt
    19621 tacaatcatc ttgcttcttt actatagcag aaaagaaggg gagaaacaaa agaagatcca
    19681 acagacggca ctatagactg tagatgttgc catcctcatc cttcaaacgg taattaccga
    19741 tttttgtaaa tatgactgtt gtttcttttt tattttcttt tcctgaaata atatattcgt
    19801 catctttggt agtgatcttg atatctgtca gcactgtttt ttcttcagat gattcagaag
    19861 aatcagttcc ttgtgtctga tacgtgcgtg tccattccat ttttttatct gtaaatgtca
    19921 actgtcccgc ggataaatcc atatctttac gtacttttag cgtatataca ctgttggtgg
    19981 tatctgcttt ttccgctttt aagacagaag aatcggttgt ttcgtccgag gattggtcag
    20041 agccgccaaa caaatcacag ccagtcaatg tcaaaagtcc cgcagacaaa ataaatagta
    20101 cgattaattt cttcaaaata cttcccccat ttgataggat aaagaaatta taccattgga
    20161 atgtaaaact tcaaacggat ggaagagaaa aacgcacaaa acacccacaa atagagtaaa
    20221 ttttagaata aaattttaaa aaggcaaaaa atccgccact ttcagaaagc gttttaaaaa
    20281 agtgttgcaa tcttttaaaa aaatgctata ttaaaaatga accggttcaa atacttgttt
    20341 aagtataatt aatagtgaga ggaagttgct ctactgaaga ctttagttat atggatattg
    20401 atgacaagca acactatgca aagaatgcgg tcatcataat cgaacaacaa gaaagagaaa
    20461 tcaaatgtaa ctggcaggag tgagaatgta tggcatcgat ccgtgatgtg gctaagatgg
    20521 ctggtgtttc agtgggaaca gtttcccgtt acttgaatgg tcagcaactg aaggaaaaga
    20581 acatgaaaaa aatagcagaa gcgatttcag cgttggacta taaagaaaac atcattgcta
    20641 aagggttgaa gaataatcga agcttctcta ttggactttt gataaacggg atttccagcc
    20701 gttttgggtc ggaagttgtc tcagggatcg aaagagtagc tgaagaaaat gggtatagct
    20761 tattattaag cgggtttgcc gatcagccag aaaaaattga acaaaaaatt gaatacctga
    20821 tgaagcatgt gatcgatggg ctgattgtct tcttatccga agaagagtgg tctggatttg
    20881 aaatgctcac aaaaatgagt attccagtta tcgcattgaa ttgtccagaa ggaccagcag
    20941 gtattgatac aatattagtg aatgatcgag aaagtgtagc aaaagtgatc gctcatcatg
    21001 gtgaacaagg acataaaaaa atcggattta ttgctgccac tcaaacagac tacgtagcaa
    21061 gagaacgttt agcaggagcc aaagaagcgg tccaaaatga tccatcgatc caattagagg
    21121 tctttacagg agactattct agaaaaagcg gctattacgg ggcaaaagca ttattagaaa
    21181 aaggggcaac tgctattttt gtcagcaatt acaatatgtc gattggtgcc atcgaatgtt
    21241 tcaatcaaga aggtgtcaga atcgggaagg atattgcgtt cagtcactat gattatttag
    21301 ataaaaacga tctgagtatc cttccgaaaa tcacgatcac gccaccaaca gataaaatcg
    21361 gggaacgagc agcagaacag ctgttaaaac gaatcaaaaa agaagaggaa gaaaatgaaa
    21421 aagtgattgt gatggaaaat atcatacatg gaatcaacta aaacgaacta aaaaagaaaa
    21481 agaggtgctt tgatgaaaaa atatcaattt ccagcaaatt tttggtgggg ttctgcagca
    21541 agtggcccac aaacagaagg aaggatttca ggcgatggaa aaggagagaa catttttgat
    21601 tattggtaca aaaaagaacc gcaaaagttc tttgatcaag taggacctga aaaaacctcg
    21661 caagtttata cgaagtatca agaggatgta cagttgatga aacagactgg gcataattca
    21721 tttcgaacat ctatccaatg gagccgtttg atacctgatg aatctggaac aatcaacgaa
    21781 gaagccgttc gtttctatca tcgttatttt gatgagttga ttgaaaacgg tatcgaaccc
    21841 tttgtgaatc tgtatcattt cgatatgccg atgtatctgc aagaaaaagg cggatggttg
    21901 aaccgagaaa cggttgaagc ctatgaaaaa tatgccaaaa gttgttttga attgtttggt
    21961 tcgaaagtaa aaaaatggtt tactcataat gaaccaatcg ttccagtcga agcaggttat
    22021 ttatatcgat ggcattatcc agaagagtca gatatgaaaa aagcgatcca agttggctat
    22081 catgaagcgt tggcttcagc gttagccatc aaatcttacc atgaaatgga gcaaggtggt
    22141 gttattggta tcatcttgaa tctgacacca agctatccaa gagacgaatc aaaccctgaa
    22201 gatgttaaag cagcgcaaat agcagatgcg ttcttcaatc gttcattcct tgatccagct
    22261 gtaaaaggcg agtttccaag tgaattgatt gagatagtaa aagaacttga tctcatgcct
    22321 gtgatccatg cagaggattt gacggttatc aaagaaaata cagtagatct tttaggtatc
    22381 aattattacc agccacgccg ggtaaaagca aaagaaacac caatcgatac taaaaacggt
    22441 ccaatgcctg aggattattt cgacaattat gaaatgcctg gtcgtaaaat gaatccttat
    22501 cgcggatggg agatctatga acaaggaatc tatgatattt tgatcaatgt acgagatcat
    22561 tatggaaata tcgactgctt tatttctgaa aacggtatgg gtgtagaaaa cgaagcacga
    22621 tttatcaaag aagatggcat gatccacgat gattatcgga tcgaatttgt ccaatctcat
    22681 ctgaaatatg tccatcaagc cattcaagaa ggtgccaatt gtttaggcta tcatatgtgg
    22741 acatgtatgg ataactggtc gtggaccaat gcatataaga atcgatatgg ctttatttca
    22801 gtagacttag cacaagatgg aaaaagaaca atcaaaaaat cgggtcattg gttcaaagac
    22861 gtatcagata aaaacggatt tgaggcttaa gaaagaatag ttgagagcaa atgcgtgaaa
    22921 ttctccattc ttaccatttt gtgaaaaaga agttcaatta tttacgatct ttttcaactt
    22981 ttttgtcttt ctatttcatt ttttgtatat aatagaatag tcaaaattaa ataggagtgg
    23041 aggaaagcat gaggaaaaaa agaccgtttc ttctcttttg gttattcggt tttttcttac
    23101 tgcttatttt atccgcagca ttttcaggaa aaatgacagc agatcttgct cagaaagaaa
    23161 cagaaaaaat cgtagcaagc aaagaatcag tagataattt caaaaaagaa ttctcaactt
    23221 attggtgggc ggagtataaa ataactgcca attacgaaaa aggcgaattg gacatttatt
    23281 tgccaaaagg aaaagaagaa aatcaattta ttaatatcga tgacgtactt agtgcgattt
    23341 ctctcagaca atatcaattt aaagacaagc atttgaccgt tttgcttttg actcatgatg
    23401 gcgacaaagt agcccgtttg aaaaatggaa aagtgatcac cgcttattct gaaaaagtag
    23461 acaaaaaagt ggtcgccgat cgtttagcgg aatggaaaaa gacaaatgaa caagtcaaga
    23521 aaacaactgt ctcttctcaa aaaaacgagt cgacaggaac aagtccttca gattctggca
    23581 gtggacaaac gataacggga agtgaagaaa atcaagcctc aattattacg aatgagtcat
    23641 cacaaggaca atatcaatcc tatggacaat gaaaaacaga ggttgtgaaa tcagcgtttt
    23701 gacctgagta ttagagaaac aaacggcaaa atagttcctc atattttgcc gttttttgag
    23761 ctaataccga aggtcagctg attgaacacc gtgtatcaag aggtcttgag cctgccgttt
    23821 aactctgagc cacaaggagc aatttcaaaa aatagctcct tatatttttc gtttggttgg
    23881 gcttattacc gcagatgcaa gcttttgaac accgtttacc aagaggttgt aaaattagcg
    23941 tcctgacata atctaatacc gagggtcagc tgattgaaca ccgtgtatca agaggtcttg
    24001 agcctgccgt ttaactctga gccacaagga acaatttcaa aaaacagctt ctcatgtttt
    24061 ataaatattg tgacttgtgg ccgaggagtt ggcaggagaa caccgtttaa ggaagaagtt
    24121 gtgaaaaagc tgtcctgcat caagaaataa ggagctgcga caagaatttt gtcacagcct
    24181 ctttttttgt acgaataccg agaaactggc ttttgaatac cgtggagtga agcggtcacc
    24241 acaagacttt tatctcttca ttagtttaat cgcttacatc gcagcggttt tatgctgtga
    24301 aaagtggacg gaataatctt tagaaagaat aaaaaaatct taagataaca acttttttag
    24361 gaaattttca tttttcagga gcaaaataag aaaaaatgtg aatagagaga agtgatatac
    24421 gtgacacaac ttaaaaaatt gtgccaaaat cgtttagcaa tcgttttgac agctgttttc
    24481 tttttctggc tgaagacgat cattgcctac tatgcagatt tttcattagg tgtggaaggt
    24541 acgatccagt actttatctt gtggatcaac ccaattgcga ctaccttatt gttttttggc
    24601 ctttctttgt atattaaaaa gccaaagcca gcgttagcta ttcttttgat cattgatatt
    24661 ttaaatacgc ttttgttgta cttgaatatt attttttatc gggaattcac agatttcatt
    24721 acagtcaaat ctgtattagg cttttcgaaa gtgtcgcaag gattgtcagg aagctccttt
    24781 tctttgatga agccccatga tgtcatctac tggctggata tcgctgtgtt cattgggcta
    24841 ctggtttggt tgaaggtcaa gaaaatacct atcaaaagca accctgtcgg caaaccgatg
    24901 gctatcgctg ttacctgtct ttcagggctt attttttcag gaaatctagc gctgagcgaa
    24961 gccaaccgtc cacaattgct gcagcgaaca tttgaccgtt catatattgt caaatatcta
    25021 ggaatcgatg cgtatactat ttatgacgga atcaaaacag gaatgactag cagtgttcgc
    25081 gcgcatgcaa gcagtaatgg gattgatgaa gttttagatt atacaaagaa acattatgca
    25141 gagccaaatc ccgaaacatt tgggatcgct aaagggaaaa atgtgatcgt tctccattta
    25201 gaaagtttcc agcagttttt gattaatatg aaggtagacg gacaagaagt cacaccgttt
    25261 ttaaatagta ttttccagaa tcaagcaacg atcagctttg acaatttctt tcatgaagta
    25321 ggacagggaa agacaagtga tgctgaaaat atgttggaaa ctggcacatt tggtttacct
    25381 caaggctcat tattcacaga acttgggtca gacaatgtct ttcaagccgc accagcgatc
    25441 cttggacaaa agcaaggcta tacaagcgct gttttccatg ggaatgtagc tagtttttgg
    25501 aatagagatc atgtatataa aaacttaggt tatgacaatt tctttgatcg atcttatttt
    25561 gatgaatccg acgaaacgtt aggttatggg atattagata aagatttatt cagagaatct
    25621 gcccaatatc ttgagcatct acaacagccg ttttacacga aatttctttc cgtaaccaac
    25681 cacacgcctt attatacgga tgacaagaat ttcgactttc catctttgaa tactggcaat
    25741 agcacagtag ataattatgt tcgaacggca cattatctgg atcaatcatt ggaacaattt
    25801 tttacgtatt tgaaaaaatc tggtatttac caaaattcaa tctttgtgat ttacggggat
    25861 cattttggga tctcgaatac agataataaa gatttagcaa gcgctcttgg taaagatcca
    25921 gatacatggg atgaatttga caacgcacag atgcaacgag tgccattgat gatccatatg
    25981 cctggttata ctaaagggac agtcaatcat gaatacggtg gagaaatcga tgttttgccg
    26041 acattactac atctattggg aatcgatgat aaagattatc ttcattttgg tacagactta
    26101 ttatcttctc agcatgatca agtagttgct tttcgaaatg ggaactttgt gacacccaaa
    26161 tacaccgtcc taggaggaaa agcttacaat aatcagacag gagaaatcat cgatacgaaa
    26221 gcagcaggaa tcgatgagga aatctccaaa gatcaggaaa aagtcaagac tgcgttaagt
    26281 ctttcagata agctgaatca ggaaaatctc ttgcggtttt atgtgccaaa cggatttgag
    26341 acgattgatc cgaaaaaata tgactataaa aatgaagcgg aacgtaatga agcaatcgaa
    26401 aagcaaaaag gcgaacattc gacaagtgtc ttttcaaaaa atggtaataa aacaacaacc
    26461 aacttatatc cagtaacaag aacagacgtt gaacagcaaa aaaataatca ataaacaatc
    26521 aaaccaacct tcagtagtta gacaacattc taatgcttga aggttggtat tttttatgaa
    26581 aaaaacattt ttttattttt ttcgtgtatt tttccttttt aacgtatcta tttattgaat
    26641 gtggaaaaat ttaaattaaa aaggagaact attatgtatg atggtgaatt gatcaagaaa
    26701 cttcgagtca acaagaagtt gacacagtcc caattagcgg aaggaatctg ttttaagact
    26761 tctctagtcg ggattgagac aaattcagtg aaaaaaatgt catttgtaac attgagatca
    26821 tttttagagc gaatgaacat gacacttgca gaatatgaat ggatgcgaaa ccagctggat
    26881 gaaccaataa agttaaagaa aaaaagacga atgctagatc gggtacaaga agacagcttt
    26941 gatccttata aagagatcag caataaccgt aggcatttta agaaaacaga agatctgtat
    27001 tatttggttt tgaatcttca catggtcgaa aaaacagaag tatctgagac agaatttgaa
    27061 tttttacgtt atgaatgccg aatcattgaa gaatacttga tgcagatacc agactatggt
    27121 caatttgaac tatctattct agctgatttt ccttggatat tcagtgatcg gttcattgag
    27181 aacaattatt tgaaaatcaa aaaaagaatg cgacgattag cacagttgaa caatcatgag
    27241 cagtatctct ttactttttt aatgaacctg acttcgtttt atatagaaaa tggtcggttg
    27301 aaaaaggcac gtagtgtgaa tgaagatatg aaacggagct tagcacataa agaaagaagt
    27361 acagtgatct atgaaacatt gatggcagat tattatcaaa gattgatttc tgctgcttta
    27421 ggggaagaaa agacgaaaga ctaccaagaa ttcttcacag tgctcgaata tatgttagga
    27481 aagaatcaac ggaggtcctt agagaaaaaa ctgctggcca ttgctgaacc cagcaactag
    27541 accagcagtt tagaatttgt atcacttcgc cgctttttca gacggtttgt ttatatcgaa
    27601 tggcgggaaa aagcgggcga tctcttttac cgcagcatca cgggaatcag aagcgtgaat
    27661 gatgttttca gtccccggca gcgcgtagtc tcctcggatc gtacctggaa cagcatcggc
    27721 agcttttgtt gctccaacca tcttacggac gatatcgatc acttcctcgc cgaccagcac
    27781 aagataaacg acaggaccgg aagtcatgta atcgatcagt tcatcaaaaa aactgcgctc
    27841 agtcaagtgc tgataatgtt cttttgctaa ttcaggagtc atagtttcaa acttcatttc
    27901 agcaatggcc agtccctttg cttcaaaacg ttggatgatg cttccgacta aatggcgtct
    27961 cacgccgtct ggcttgataa taacaagtgt tctttccata acagcacctc gtttcctact
    28021 atatttctat tatagcggga ggtggaaaat gagacaaata aaaccgcgtg agacaggaat
    28081 ataacgtgtg tattttaaaa aaagtatgaa aaacatggaa aattgatttg acatcctctt
    28141 ttttagttgt tatattaacg aaggaaaacc gaataagatt tgttaggtga ggctcctata
    28201 caaacatagg ctactgccca aaaacgtcga gagacgccaa tgggtagaac aggaattgtc
    28261 gagataaggc ttttcataag gtagctaaaa gcaggtatct gctttttacg ttgtacagtg
    28321 tcaaaactca acgatgagat ctgagaagct ttttttaata tgctttttca aacctttcgc
    28381 tgagtatttg gcgagaggtt tttttgttgc cgcaaatccc catcggtgaa aaataaaaag
    28441 gaggtggtct aattggaaaa ataactattc gaaaaatgat tggaagtcat gaattaaaat
    28501 tacttagtaa aggagatgag aacaaatgga tgacagtcat tcgaaaaaca aggatctaga
    28561 ggaaaggcca gggctattat ctatctgttc tcagcccctt tcagtttttt ctctatcgat
    28621 ccgatgaata gctaaagacc aaaagcagcg agaacagctg tcttttggac acaaaggaga
    28681 gattgagatg gaaaaaaaga aagatacaca aaaaatctat gcagcttttg ctggaaaaac
    28741 gaaacaagaa ttatttgaac tattccatgc atcagcaaat ggattgacag aagaacaaat
    28801 cgaacgttca agagaaaatt acggtgaaaa tgcgatttca tatggcaaaa aaacaccatt
    28861 cattgtagaa atattgaaag catacatcac gccatttaca ttagtgttga tcgcattagg
    28921 aattatttca ttcatcacgg aatatgtatt agcagctcct ggagataaag atcttttcgg
    28981 tgtagccatt atcttcacta tggttatcgt cagcgggaca atgacgttga ttcagtccgt
    29041 acgttccaat caagcggctg aaaaactgaa atcacttgtg aaagtaacgg cagctgtaaa
    29101 acgtaatgga aagtacacag agattcctat ggaagaaatc gtctgtggag atcttgttcg
    29161 gctttcagca ggggatatga taccagcgga tatgcgttta ttgtccgcaa aggatttatt
    29221 catttcccaa gcagcattga caggggaaag ctatccagta gaaaagcatg cgaaagtatg
    29281 tgaagacgat cagcaaagcg aaacaagtta cgaaaatctt gtctttatgg gaagtaatgt
    29341 catcagtgga agtgcggaag gaatcattgt ttctgttgga aatgaaacac tatttggtca
    29401 tgtagcagaa acgctatctg aaaaaccaat tcaatcaagc tttgaattag gcatccataa
    29461 aacatccatg ctactgatca aatttatgtc attgatggct ccagcagtta tcgtcatcaa
    29521 tgggcttaca aaaggggatt ggctagaagc ttttcttttt ggactatctg tagcagttgg
    29581 tctaacacct gaaatgcttc caatgatcgt tacgacgaat ttggtcaaag gagcaagtgt
    29641 gatggccaaa aaaggaacag tgatcaaaaa tttgaatgcc atccagaact ttggtgcaat
    29701 cgatgtgtta tgtacggata aaacggggac attgacacaa gataaaatca ttcttgaata
    29761 tcatatggat tgtgaaggaa atgaagatga tcgtgttttg cgccatgcgt atctaaatag
    29821 ctactatcaa acagggctga aaaatttgtt agatgtggct atcattgatg aagcaacaga
    29881 aactttagat acagataaga tcaattatca aaaagtggat gaaattcctt ttgattttga
    29941 acgtcgtcgt atgagtgttg tggttgctga tccagctggt aaaacacaaa tgatcacaaa
    30001 aggcgcgatt gaagaaatgc tgaacatttc taaatttatt gatcttggcg gtactgttac
    30061 accattaaca aaagaacgaa aagaagccgt acttgcaaaa gtacagtcac ttaatgaaga
    30121 tggcttacga gtgattggtg tcgcacaaaa gaccaatcca agcgtcgttg gagaattttc
    30181 agtcaaagat gagtcagaca tggttttgat tggttatctt gctttccttg atccaccaaa
    30241 agaaacaacg aaacaagcgt tgaaagctct gaaagaacat ggtgtagctg taaaagttct
    30301 gactggggac aacgaattag ttacacgttc agtgtgccgt caagtaggtc tagagatcaa
    30361 tgaattggtt accggtgaga aaatctcgga aatgagtgat catgaattag cacaagtagc
    30421 tgaagaccat gaagtattcg taaaattgaa tcctcagcaa aaagcacgtt tgacgacagc
    30481 tttacgtaaa aatggtcata ctgtcggatt tttaggagat ggtatcaatg acgctcctgc
    30541 aatgaaagtg gctgatatcg gtatttctgt agatacagct gtggatattg cgaaagaatc
    30601 tgcagacgtg atccttttag aaaaagattt gacgattttg gaaagaggcc ttctttctgg
    30661 acgagaaact tttgggaata tcatgaaata tatcaaggct acagcaagtt ctaattttgg
    30721 caatatgttt tccgtgctga ttgcaagtac gttcctgcca tttttaccaa tgctgccttt
    30781 acaacttttg tttttgaatc tgatctatga tgtttcctgc atttccttgc catgggatcg
    30841 gatggataaa gaatatttgg acgagccaaa aaaatggaat gcatctagta ttggggaatt
    30901 catggtttac tttggtccaa caagttcgat atttgatatt actacttact tgttgatgta
    30961 ttttgttatt tgtcctgctg tagtgggcgg aagtttccat acattggata gttcacagcg
    31021 agtgattttc atcgcattat tccatgcagg atggtttgtc gaatcactat ggacacaaac
    31081 cttagtgctc catgcactgc gtacaccgaa agtaccattt atccaaagca acgcaacatt
    31141 tgccatgttt gttatcacta cattaggaat tattgtcggt tctgtccttc cttttacaag
    31201 ttttggtgct gaattaggct tgatgccact tcctggcaat tactggttat ggttatttgc
    31261 tacagtcatc gcttatctgg cgcttgtgac agctgtgaag aaaatctata tccgtcgatt
    31321 ccaagaactc ttataaacga gaaggctggt tctttgcttc tgttcttttt ggaccagtcg
    31381 tgctaaacta tgtgtattgt tttggaaagg attaatgtat agatgaaagg tgcaatattt
    31441 gatttagatg gtttgttagt tgacacagaa aaaacgtatc gtgatggctg gctttgggga
    31501 tttcagcaat ttgatatcca tatcccaaaa gaaatcgttg acgcttgggg cggaaaaaac
    31561 tggaaacaaa gttttgatat tttagtgaaa gcagcaggta gccctgaaaa agtacaggaa
    31621 gtccgagcga agcgtgaaga atatttttat caacagctga caagcggagc catccactta
    31681 aaaccatatg caaaagaaat attggcagac ttaaaagaac gtcatatgaa actaggcatt
    31741 gctacgacca ctgttacgaa aagagcaaca gatatcttgg atcactttaa attagctgat
    31801 tacttggaca cgtttacatt cggagacgaa gtgtcggaga acaagccttc accgattcct
    31861 tatctgacag ctttggaacg aacaggatta gaagctagtt cagcctttgc agtggaagat
    31921 tctttagtag gagcaacagc tgcttcacga gcaggattag gcgttgtttt gatacctgat
    31981 tccagttttg tccgaaatta taccgtcgaa gaaaaggaaa aattgaaact tcttgcggaa
    32041 ggaaaagatc ttcgaactgt tattgaaatg ttaaataaaa aaacagaaaa ataaatatag
    32101 aaaaaataat ttgaatttcg tacttgactt ttcagttttc ctcatgtaga ttaatcccaa
    32161 caacaaaata tcgatttcgt tgaaaaggaa agtaagtttg gaaactttgc agagaggctc
    32221 tggctggtga gaagagtcaa gtaagaaaag ctgaaaatgg ccttggagaa taatttgtcg
    32281 ataagtaggc aaattcggaa ctgttcgacc gttaaaacga acgccaatct ctctttttga
    32341 gatgttgggc ttgagggaag gaaacttccg aaaaaaggtg gtaccgcgat acgtcgtcct
    32401 tttactgaca tagtttatgt tagtaaaagg actttttttt tgaatattat ttgcattgaa
    32461 atggtaagta cgatagaaga acctttgaca gagagcttcg gtagctgaga agaagcaagg
    32521 aacgactatt ggaaaatggc catcgagcaa ttcatcccga gcatagatgt aaggcgatga
    32581 cggctgcgac cgatatcttg ctagagtatg atcgtacttg aagaggagtt gtgtgtgaac
    32641 acagcttgaa catgaggtgg taacacgcaa aaaagcgtcc tcaagaaaat gacattttca
    32701 ttttcttgag gacgcttttt ttagataagg aggaatttta atgattgaat tgcgagatgt
    32761 ttctgtatgt ttcaaacaaa aagaaaaaga gatacaggca gtagattctg tagatttaac
    32821 gattgaaaaa ggcgatgttt atggaatcgt cggttactca ggtgcaggga aaagtacgtt
    32881 agttcgtgtg atgaatttgc tgcagaaacc aacagcagga gaagtcattg tcaatcagac
    32941 gaatctgagt cagctctctc ccaaagaatt gcgaaaagaa agaaaggcca tcggtatgat
    33001 ctttcagcat ttcaatctaa tggaaagccg aactattttt gacaacgttg atttttcttt
    33061 aaagtattct ggaaaatcga aacaggaacg tcgtcaaaaa gtgaatgaac tactggaact
    33121 tgtcggtttg gaagaaaaag catctgctta tcctaaacaa ctgtcggggg gacaaaaaca
    33181 acgagtagca atcgcacgag ctttggcgaa tgaacctaag gtattattat gtgatgaagc
    33241 aaccagtgca cttgatccga aaacgaccct tcagatcctt gcattgttaa aaaaattgaa
    33301 tcgccagtta ggtttaacaa tcgtcctgat cactcatgag atgcaagttg tcaaagaaat
    33361 ctgcaacaag gtagcggtta tggaagatgg aagaatcgtt gaaaaaggta gcagcatcca
    33421 gatcttcagc aatcctgaag aagaattgac caaagatttt attcgaacag ctactcatct
    33481 tgaccaagca ttagaaacga tcattgccca ttctgctttt gccgatcaga tcgccaataa
    33541 atggctggtg gaattaagtt atattggaaa tcagacgaac gaacctctaa tcgctcatct
    33601 gtacagcaaa tatcaagtaa cagcgaatat tttatatggc aatgtagagt tattacaaga
    33661 aacgccgtta gggagtttga tcgttacttt agctggagaa accaaacagc ggaaaaaagc
    33721 attggattat ttgatcaaat ctggtgtaaa aataaatatt ttacaaaaaa acgatttaga
    33781 tagtcgtatc aaagtgatcg aaggaggaag ataaaatgac tgatttcttt aaaacatatt
    33841 tgcccaatgt ttatttgatt ccggatgaat ttgtcgaagc gacgaagcaa acactatata
    33901 tgtctttttg gacagcattc attggcggga tgattggaat catcttaggt gtgacgttag
    33961 tagtgacacg ccctaatgga ttactagcta accgtctgct atttgaaata cttgataagc
    34021 tgatcaatat cattcgttcc atccctttca ttatcttgct ttctctttta gcattgacta
    34081 ctcgtttctt agttggaacg acgattggtg cgaaagcagc tttagtgcca ttgatttttg
    34141 gagttgttcc gttttttgct cgtcaaatcg agaacgcact gctagaagtt gatcctgggg
    34201 tgatcgaagc ggcagaagcg atgggaacca gtccaatcgg tatcatttta cgtgtgtatt
    34261 tgttagaagg tttgcctggt atcattcggg tctcggcgtt gactatcatc aacgtgatcg
    34321 gactgactac catggctgga gcagtaggtg caggcggatt agggaatctt gctatatcga
    34381 gagggtataa tcgattccaa acagatgtaa cgattgtggc tacattattg attctaatct
    34441 tagttttttt aagtcaattt atcagtaatc aactgattaa aaaaattaca cattgaaagt
    34501 ggaggagaaa aaatgaaaaa aattattcgt tttgtagtca ttacatttgt agcaattatt
    34561 tggttggcag ggtgtagcca aggaaaagca gatacaactg acaaaaaaga aactatcgta
    34621 aaactaggga ttattggaga agatacagat gtatgggaca acgtgaaaga ccgtttgaaa
    34681 aaggagaaca ttaatttgga atacgtgaaa tttacagatt acaatcaacc aaatgtggct
    34741 ttagcagatg gatcgattga tttgaattct ttccaacatc aattcttttt agacaattac
    34801 aataaagaac acggaactga tttagtatcg atcggaaata ctgtcaatgc accgctggga
    34861 atttattcta acactttgaa ggatgtagct gatatcaaag acggagcaaa agtagcaatc
    34921 cctaatgatg ttacaaatgg cggcagagct cttctgttat tgcaaactgc tggattgatc
    34981 aaagtggata aggcaaaagg acaagcgccc actgtttctg atattaccga gaatagaaaa
    35041 aatctagaca tttctgaatt agatgcttcc caaacagcac gtgctttgtc agatgtagac
    35101 atttcagtga tcaatagcgg tgtagctgta gatgctggat ttaatcctgc ttctgatgcc
    35161 atcttcttag aaccagtgga tgaccatgca cgtccttatg tgaatatcat cgttgcaaga
    35221 aaagaagatg aaaacaataa aacgtatcaa aaaatagtag acgcttatca aacagaagac
    35281 acagtcaaag taatcgagaa aacatcaaaa ggatcaagcg tgcctgcatg ggaaacattc
    35341 gggagaaaat aagaaaaatg actagcatag gaggaatttt ttatgacaac aacaatctca
    35401 acaaaccaat tgaaggatct aatccaatat cgtttaccag aatatcaagc acttgctcta
    35461 gatatccacg aacatcctga agtaagtaac tatgagtttt attcctcagc agcgttgatt
    35521 aaacaattag aaaaagaaag gttttgggta gaaaaagaag tagctggaca ccgaacagcc
    35581 tttgatgcac gttatatttc caataaaccc ggtcccacaa ttgccttttt ggcagaattc
    35641 gatgctttac caggtattgg tcatgcatgc gggcataatt tgtttggtac ttactccgta
    35701 ttagccgcca gcatagtgaa gcaattcatt gatgaaactg gtggagaaat ccgtgtatat
    35761 ggcaccccag gggaagaagg gggcgagaac ggatcagcta aaggcagctt tgttcgagaa
    35821 ggtttttttg aggatgtcga tgtagcgtta tgcgtccatc cagcttttcg ttatgggaaa
    35881 acgacagaat ctttagcgaa tgatccagtc gacatcaaat tttacggtgt cgcttcccat
    35941 gcagcagcag cacctgaaaa aggagtaaat gctttggaag cattgatcca agtgtttaat
    36001 ggaatcaacg ctctacgctt gcaattgcca aaagatgtca atatccatgg gatcattaca
    36061 gacggaggag tagctgccaa cgtcattcca gattacgctg ctggacgttt ctatttacgt
    36121 gcaggaaatc ggcaaacatt agataaagta tatgaaaaag tggaaaatat cgtacgaggt
    36181 gctgcgataa gtacgggcac gacttttgag tttggattgt tccaaaatgc agttgatgat
    36241 gtcatcgtta ctccttcttt tgatgactta ttcttccagc atgcaaaaga agcaggtgtt
    36301 ccagatgaag aaatagaaac ggaacaaaga acaagtctcg gttcttctga tgtaggaaat
    36361 gtcagccaag tcataccaac aatccagcct acggtagcaa tctctgagac atatattgcc
    36421 ggacacacag aagaattcaa agctgctgct aaaagtgaca aaggccttgc ttctattgcg
    36481 attgcagcag aactactcgc caatacagct cttgatctgt tgaaagagcc aaagctatta
    36541 gaaagaatca agcaagaaca tcaagaaata gtagcaagaa aaaaacaaga gggcgctact
    36601 ttctagtaaa aaataaactt gtgtgtgaaa gttataaaaa taattttcag aaaaatgaaa
    36661 aaatattttc tttttcattg acaaatgatc ttatggaagg taaacttcgt acaagtcaac
    36721 ttttcacgca gtagcggtta ggtgttttgt tcagaaagtc ggtggtcgct gtgaaccgat
    36781 caagcctaat tgacgaatta catggaaagt ccatacattt gaataaaagt ttgaaagtga
    36841 aaaacagatc tgtttttaat taaggtggta ccgcggagtc attcgtcctt agtttaaaac
    36901 aggtcttttt atttttataa aggaggaatc gcggatgaat tgcggcggag taatccgata
    36961 tcttaacttt tttagaacat aagaacaact aaaaaaggag agattatcat gggaaaaaaa
    37021 ggaacaccaa gttttttaga agcaacactt gtattaatgg ttattgtttt aggcattgcg
    37081 attggcgttg tcggattaaa ggtctcacct aatatcacga tcttagcagc gatcggaatg
    37141 gtcatgttgt atgcagtgat aaaaagatac ccgacagatt ggctgcacga aggaatcatc
    37201 aatggcatca aaccaggaat catcccaatc tttattttta ttttagtcgg ttcattgatt
    37261 gctgtatgga tacaagcagg gatcattcca actctaatgg tcattgggtt caaattgatc
    37321 agtgtccagt ggtttgtccc ttctgtattt gttgtgtgtg caatcgtagg tagcgctgta
    37381 gggagtgcct ttactgttat gtcaacgatt gggattgcat ttttcggtat tgggacaaca
    37441 ctaggcttaa atccagcgct ggttgttgga gctattgttt caggtgccgt ttttggagat
    37501 aagatgtctc cattatccga atcaacaaat ctagcagcag ctattgttga tgccgatttg
    37561 ttcaaacaca tcaagcatat gatgtggtca actgtcccgg catttgttgt ttctttcttt
    37621 ttattcatga ttttaggaca cacaagtaaa gccgcaagtc tttcatctat tcgtgaagtg
    37681 actgatgttc tagaagcaaa ctttactatt tcaatctggt cactgattcc tttattttta
    37741 atgttactgt gtgcatggaa aaaggtgccg gcaattttaa cgattttgtt gaatatcgct
    37801 gtagcagtag gaatgatttt tattcaaaac ccacaaactt ccttaacgaa gttggctggg
    37861 gtcatcgaat cagggtttgt ttcaaatact ggaaaccaac aaatcgattc actgcttagc
    37921 cgcggaggaa tcgaaagtat gatgcctact gtgtcattga ttattctaac tttgtcatta
    37981 ggtggattgt tgatcgaatt tggcttgatc tcaaccgtga tggaacgagt atcaagaaag
    38041 atgacgaaca caccaaattt gatttttaca acattgatga caagtatcgg agtaaatatt
    38101 tttattggtg aacaattcct atctgttatt ctgcctggta atgcatttaa agaaacttat
    38161 gaaaaagcag gatttgatcc aactgtatta ggtagaacgc tagaagatgg tggaacagta
    38221 atcaactact tagtgccttg gggaattgcc ggaagtttcg tagcaagtac atttggtatt
    38281 cctacactca cttacttgcc attcgtattt ttcagtttgc tttctcctgt gttttcgatg
    38341 gtcagtgcct tcacaggttg gggaatctca aaaaaatttg tgaaaccgca agaagcagta
    38401 agcaagacag cgcaattaga acaataaaaa cataaaaagg gattgtgaca agacttttgt
    38461 cacaatccct tttacgaata aacggtattc aaaagccagt ttctcggtat taagccaaat
    38521 ttatacaaaa atgtggaaag ccatttcaca aaatttttct taatacgcga aactaagcga
    38581 ctttttcaca acctctcgat aaacggtgtt caatcagctg accctcggta ttagctcaaa
    38641 aaatggcaaa atatgaggaa ctatttttcc atttgtctct ctaatactca ggtcagaacg
    38701 ctgattttac aacctcttaa taaacggtgt tcaaaagctt gcatctgcgg taataagcct
    38761 aaccaaacga aaaataggag aagctatttt tgtttggctg ttcttattac ttgatgcagg
    38821 gcagcttttt cacaacccct tattcacggc gttccagaag tagcttcttc ttatttcttg
    38881 aagctgataa cttctggaac atcctctttt tttatttgga cggataatgg tcattccatt
    38941 tctttgatca tttttttcat tttgtcgatc tcttcttctt gtgcctgaat gatttcttcc
    39001 gctaattttt ttacttcagg atcttttaaa tcagctcgtt tacttgccag gatagcgata
    39061 gaatgatgag gaatcatggc cttcatccaa gctgtgtcgt tgacagtagt ttggcttcgt
    39121 accataaaca aggaaccggc aaaaacaaga agactagcgg ctaagatcat aacatttatc
    39181 tttttgtttt catacatttt ccacatgaac aacagcatga tgactgccat gactgcaccc
    39241 atcatcaacg ccatgaatac tcgtgtacga ctgaagaaga tatgatcgag cgcatagaca
    39301 ttcaagtaca tcataccaaa cataactacc gttgaaacac cgatcatcaa caaaaacttc
    39361 caatactttt tcatttaaaa caaactcctt tttttcattc ctttcttcta tcatgtcatg
    39421 agcatggaga acaatcaatt attttgcaca gagggttttt gaacaccgtt taccaagagg
    39481 tcatgagact gtttattcgg aaaaaggagt tgcaacaaga cttttgtcac aactcctttc
    39541 ttcaatagct ccatctattc cacataaata aaataaaatt ttctatcgat ttcattcatt
    39601 cgtcaatacg aacgcatcat tctttttttg ataactgaaa taaatgaaca ggtaaagcag
    39661 gccagtcaat acagttaccc caatggagtt cgtgaacagg ataccaagga aaatcaatcc
    39721 taagacagct aatgcaaaaa agctgattcc tttatcccat aatgttcctt ttcttagtgc
    39781 cagtacaaag gatgccgcac tgataatgat ccagatcaat aatactgtat acgaaaggga
    39841 acctgccaga tagccaaata atttgtcacc gacaaaataa gaaagagcta caccaagata
    39901 aagaacactg gtacaaaata aaacagcacg ttgcggtact tgatgcttgt ttaaaacagc
    39961 tagttttcta gaggaacctt ttcgatcctt caaacggaag aacaaaattc ttgaagatgc
    40021 atacacaccc gaattgatag atgagaacaa agcaaggaca atcacaaaat tgacgatatc
    40081 cgctgcaaat gggatattca ttttttcaaa taccatcaca aaaggactga cattcgtgcc
    40141 tgctaaagta ttccaaggaa aaagaatcaa aagcaaaaac aaaggaatga tgtaaaagga
    40201 aatgattctt cccatgacac ctttgattgc tttaggaatc gcacgtttag gatcatctgc
    40261 ttcgcttacc gtgatagcaa tcagttcaga accgccgtaa gaatagataa ctacaagcaa
    40321 agaactgatg attcccttca taccatgagg agtaaaccca ccatggtcag tcaattgctg
    40381 aagggtaggg aaaacgcctg tgcccaacat ttgctgaccg acaagataag tagcaaaaat
    40441 gatcagtaag ataatgacac tgattttagc aaaagccagc caatattctg tttctgcgaa
    40501 taaccgtacc gaataaagat tgatgatcgt agttaaaaca gccaacataa aaacaaatac
    40561 ccaggctgga atgtgcggga accacaactg caaaaaggaa gcagctgcaa cagcttcagc
    40621 gatgatattg ataatccaca tagaccagta gacccaatca gtaaaatcag cagaatgagc
    40681 acctagataa gggcgtatca aaccagataa tccgtgatgc gtgtcgccac cgcttaaaac
    40741 gatttttccc agacctttca tcacgatata taaaatcagc ccaccaatca aaaaagcaat
    40801 caatacagaa gggccggcga tcccaatcgc ttcgccactt cctttaaata gacctgcacc
    40861 gattgctcca cctaaagcta acatggtgat atgtctggag ctaagttgtt ttttcaattt
    40921 tttatcttcc ataaatgacc tctttctttt aaattcacaa tcaaaattta tattaagaat
    40981 aaatagcaat atttaacaat gactaatgat aacaaatttt cagacaatta caactattca
    41041 gaaaactttt aggcgaattt taatagaaga atgatttatt tcatcatatt ttcatacaaa
    41101 tgttaaattt tcatacaaat ataggaaacg ttttactggc tttttactat ttaaacaggc
    41161 tatcatagga aaaagtgaca gagaaagtga ggcggaaaag atgaacaaaa atgacgtact
    41221 gctagttgca aaaaataaat cactttattt tttgccttcc atccgctctt tagatgtttg
    41281 gtccggaaaa gatttactac agtcggaatt cattaaatca gctagacaac agttggcaaa
    41341 ttatcagctg atcgtttttt tagattatgg atttgatatg gagatggctc gacgcgtgcg
    41401 cccatttacc aatgcaaaaa ttgtgttatt tttttggaat cacttcaaag cagaacacga
    41461 ggcgatgctg aagcaggcac aagatgaacc ggcaatcgat gagatttatc attttgattt
    41521 tttggaagca aaagaacttg ggctgaaaca taatagttca ttttattcga aacatatgaa
    41581 tttttcttct agcgaaccaa agacggatct attttttggt gcaacgaata atgggagaaa
    41641 agaacgagca gaaagctata agcaggaatt tataaaaaga gatgttacga ccaactattt
    41701 cattcttcca catcgaggga atgaacaacc tggatatctt acttatgaag agtacctgaa
    41761 aaaaacaggg gaatcgcgcg ggattctgga actgttgcgg gagggtcagc aaggagtgac
    41821 tttgcggaca tttgaatcca tgtattttca aaagaaacta gtgacggata atcaagcgat
    41881 tgctcattat catttttatc gtcctgaaaa tatttttttg cttcgagaac gtgatttaga
    41941 tgaattaccg gaatttctgc atacaccatt taagccaatc ctatcagagc tattggaatt
    42001 ttatgatgca gaaaattggg caaaacgatt tatcactaac gattccacta tttatgagca
    42061 atacgaatat gacctctcct ctgttgaaaa caaataaaaa acctacattt tgaagagatt
    42121 atgtactgaa acaaacaagc acaattctta tggcatcatg agagttgtgt tttctttttg
    42181 atcttttatt gggcttaaaa aaagagaacc agctgctgat tcagctgatt ctcatcagag
    42241 agccttttat tctgcgccaa ggattccttc ttttgcatgg actaattggg tcaaaaaggc
    42301 acaatatggt gtagcaatat catatttttg tccttttcgc caaatggcac cattgatgta
    42361 gtcgatttca gtcaaacgat ggtttttgat caggtcttgg tacatagatg ggtaatgcaa
    42421 cccgatgttg tcaggatcat aagtagaagc aatatgttgg tacacttctt cttgatctag
    42481 tatgattcct tcttttgctg cgatagaagc aaattcagat acgattgttc gaaccatgct
    42541 ctctgccgct ttttgcgcgc ctagttcagc aatattacaa tccaaaatcg tgcataaacc
    42601 attcaatgtt ccatttacac aagcttttcg ccaaatggaa taacgaacgt tatggctata
    42661 gatcggattt aaatttgctt cttgaaagac ttctacaact ttttttgtaa atgcttcgcc
    42721 ttctggttcc aagttctcta gttcgatttc tccatcacct aacaatgtga cttttccggg
    42781 accagctaga ccagctgtcc acatcgtgat cccaaataag atatttttct ttggcacata
    42841 tttttccagt acatcttcat gacctaatcc atttagtaaa cacaaaacat aagtattgtc
    42901 agtaataatg gattggatcg aacaaaacat atcatctaat tgattggctt ttgttaatgc
    42961 tacgatcaag tccacgtgtt cgttgctttc gatgatttct tctggtgaat agattggcag
    43021 ttttgcgacc acttctttac cgttaaagtc agcaatcaac ccatttgtac gaatggcttc
    43081 aatgtgagca ggccaacgat cgatcaagag gacctcattt ccgctttgat gtagcatcaa
    43141 tccgattcgg ctgcccattg ctccggcccc agctattgcg attttcatgt tttttctctc
    43201 ctttattcaa tgagttccct tttagtatat agaaaaatgg aagaaaatag cattgttttg
    43261 tttgaaaaaa atgaaaaaaa gtgtcttact taagaagaaa gtgagggata cagatggata
    43321 ttttggcggc aattgattca tttaaaggaa gtgcttcttc atttgaacta aatcaggcag
    43381 ttctaaatgg ctgggaagat cgtaaaggaa gaaaaatcaa tgtgccgatt gcagatggcg
    43441 gagaaaatac tgcagaagca atctattacg cgctcagggg aacatggcgt accattgatg
    43501 ggatggatct cttattccgt cagcgaaaaa tcagctattt attgacaact tggcaaggaa
    43561 aaaacaccgc agtcatcgaa tctgcacaag cattggggtt agatctactt gataaaccaa
    43621 cggatcaaac tatccgactt gcttccagtt atgccttagg agaacttatt aaagatgcac
    43681 tgcttcaaga agtagaacaa attattgtca ctttaggagg aagcgggtgt agcgatggtg
    43741 gtctaggatt actccaatcc ttgggtgctt ctttagaagg gtacacagaa ggaaatcctt
    43801 tattatctac taaaaaactt cattttgatc gaagaaacgt tcttgtttct aaagtaaaat
    43861 tgattatcgc tgcagatgtt accaatccat atacaggtca acaaggagct gcttggcaat
    43921 ttggaagaca aaaaggagga atagatacga cgctagcttt cttagataaa caagcaggaa
    43981 agattgctca gcaaataaaa gatttctggg aggtggattt agatgatatt ccaggaagtg
    44041 gagcagcagg cggtattggt ggagcccttt ttttgttggg agcgaagatg gtttctggtt
    44101 tcgatttagt cagccaaatc actggattgg aaaaaaagat cgaacaagca gatgtcatca
    44161 ttacaggaga agggcggatc gatcagcaaa cgatccacgg aaaagttcca tacggtgtag
    44221 cagtgcttgc aaaaaaatat cgaaaaccaa taatcgctat ttgcggaagt agagaccaag
    44281 agctagaagg actttctgat tttctcccaa ttgtcttaag tattcatttg ggtccaatag
    44341 gtttagaaga agcaatggaa cacaggcaaa cattagaaaa aacaaaaatc ttaggacaga
    44401 cacttagcag atttttcacc atcctcccga agtagttgac aaaatcattt tctttatgct
    44461 atcgtaattt caaatatagg acaaagtgag ggatccgtgc attgaaaaat taagtcgatt
    44521 gatgaaagct gcattcagac tgtaccttgt ggagacaagg aagcagtgtg cagattttcg
    44581 ggattggctt ggttttttcg atgtatgggt ctttttgtgt gtatccaacc cttgcatcct
    44641 ctttagccag ttctgaaaat aggactggtc tttttttacg aggtgatgga aaatgtctaa
    44701 aatcgaaata aaaaatctga cattcggcta cgacagccaa ggcacattat tatttgaaca
    44761 agcaaatcta aattttgaca cacaatggaa actaggactt atcggacgaa acggtcgagg
    44821 aaagacaact ttactgaata ttctacaaaa caaactacct tatcaagggc aagtaatcca
    44881 tcagcaagaa tttgcctatt tcccgcaaca gacaaaagat aaagaacgtt taacctatta
    44941 cgtgttaaat gatattacgg attttgagat atgggaaatc gaaagagagc tccaattgat
    45001 gcaaacagat cctgaaatct tatggagaga attcagcaca ctatcggggg gagagaagac
    45061 aaaagtccta ctggcacttt tatttgtgga tgacactcat ttcccgttaa tcgatgaacc
    45121 aacgaatcat ttggatatct ctggtagaaa acaagtagcg gcttatttga aaaagaaaaa
    45181 acaaggcttc atcgtggtca gccatgaccg gggatttatc gatgaagtag tggaccatgt
    45241 tttagcaatc gaaaaaagtc aactggaact ttatcaaggg aatttctcta tctatgaaga
    45301 acagaaaaaa cttcgtgatg aatttgaaat ggctcaaaat gaaaaattga aaaaagaagt
    45361 cagtaggcta aagaaaacag cagctgaaaa agccgaatgg tctcgttccc gagaaggaga
    45421 taaaacaaag aaacaagtcg gattcatcga tactgaatct agacgagtga ataaaggagc
    45481 agtgggtgct gatgctgcac ggacgatgaa acgatccaaa gcaatcgtga atcggatgga
    45541 gacccagatc agcgagaaag aaaaactatt aaaagatatc gaatatatcg attctttgac
    45601 gatgaatagc caagcgtctc accataagcg acttttaagc gtagaagatc ttcaattagg
    45661 gtatgaaaat ctgttattcg agccaattca ttttacaatc gagcctcatc agcgggtggc
    45721 gatttcaggt cctaacggtg caggaaagtc atccattatc cattatcttc tgggggcatt
    45781 caacggcaag gttataggag aaaaatacca gccaaaacat ctgagcatta gttatgcaag
    45841 ccaaaattat gaagacaatc gaggaacgtt ggcggaattt gcagagaaaa accaagtaga
    45901 ctaccaagca tttttgaaca acctccgaaa gcttgggatg gaaagagatg tttttcataa
    45961 caagatcgag cagatgagta tgggccaacg gaaaaaagtg gaattggcta aatctttatc
    46021 acagccagct gaactatata catgggatga accattgaat tatttggatg tcttcaatca
    46081 agaacaatta gaacaactga tcttgaacgt gaaacctgcc atgttactag tggaacatga
    46141 tcaaaccttc ctggataaag tatctactga gattatttct cttgagagaa tctaaccctt
    46201 gtaatctcaa gaacaacaag gtatggttaa ggggaacttt gccaacgaag gagagaatca
    46261 catgctttat gtaacgagac acggagaaac aacttggaac gcacaaggat tagtttgcgg
    46321 acgggcagat gtgccgctga ctgaaaaagg acagatgcag gcacagaaac tagcagaaaa
    46381 ggtagtagat ttgcctgttc caatcacgaa aatcatccat tcccctcttc aacgagcaag
    46441 agatacagca caagcagttg ctgatcgtct cagcttgccg ctgaccgttg atgaacgatt
    46501 ggtagaaatg gattttgggg attatgatgg actgccaagc aaagatgaga actttcaaaa
    46561 agcacgttta gcgttcgctg tacgattccc aaatggggag tcagtcttag atgtgtatgc
    46621 acggatcgtt cctttattga aagaatgcat cgaagacgaa gaaaatgtct atctgttagt
    46681 ttgccataat gccttgataa gagtgatcaa tgcttatttt catccgatgc ctaatgaggg
    46741 gttctttaca ttcatggtag ataatacaga attggtttct tatgagtaat tatgtttttg
    46801 tgttatttaa ataaaatatt tcaaggcact gcttttgctt agcagtgcct tttttaggtt
    46861 atgacaattc ttttgataat ataaactcaa cctttagaaa tgaaatttaa ttattttaat
    46921 aaaagtattg aaattttgtt tctttagata tatactctag tcatagaaat gaagacggat
    46981 acagaaaaag agggataata tgtttggctt ttcaatattt atgaacgatt ctttgtcaga
    47041 agagaagaaa cagtatatcc gccaaatggc atccaatggc tttgtcggta ttttcacttc
    47101 catgcatata ccagaagatg atgtttcggc atatcaaaag cgattgatcg aactaggaag
    47161 cgaagcgaaa aagaataaac tagaattgat ggtcgatatt tcaggagagg cattggaacg
    47221 agcgggtttt tcattagaag atctaacgcc tttgaaagca attggtgtaa caggtttaag
    47281 aatggattat catatttcca atcagcagat tgccgactgg tcacatcaat tgaaaatcag
    47341 tttgaatgct agcacgatca cgcctaaaga tatcgatgaa ttgaaagaag cagaagctga
    47401 tttctcacag attgaagctt ggcataatta ttatccacgt cccgaaacag ggctggacaa
    47461 agaatggtat cagaaaaaaa atcaatggct caaaattcaa ggtctccttg tccaaggttt
    47521 tgtaccagga gatacagagt tgagaggccc actatatcaa ggattgccaa ctttggaaga
    47581 acatcgaggg atccatcctc ttgcaggcgc tctagatcta ttagcaagta acacggatat
    47641 tgtttacatc ggagatgcag gtctgtcaga aaacgttcag gagcaatttg cttctttcca
    47701 aaaagaacag acagtcttgc tccatacgga acctgtagat gaagaatttt acgaatatat
    47761 attagggaag catacgaatc ggcaagacga tgcaagagat gtcatacgaa gcgtagatgc
    47821 acgttttcga gagattccgc ctatcccagc aagaaataca gctacgcgta tgaaagggtc
    47881 tattacttta gataacgaaa agtatttgcg ctatatgggt gagatccaat taaccaaata
    47941 tgatttgcca gcggatgaaa aagtgaatgt agtagctaaa gttatcaaag aagagctacc
    48001 attgatcaac caaatcaaag ctggtatgaa ctatcaattt ataagaaaag aaggaagata
    48061 aaaatgagca aaatcaattt agagaaccta acaacggaac gcagaaacca agaaactttt
    48121 ggattagatg agatgagtat ttctcaagcc gttcaactaa tgaacaaaga agatcagaaa
    48181 gtggccgctg cggttgaaaa agaactgaca agtatcgaac cagtgattgc taacacgatt
    48241 gagtccttta aaaaaggtgg acgtctgatt tatatgggag ccggaacaag tggaagacta
    48301 ggtgtgttag atgctgctga atgcgtaccg acattcggtg tagaaccgga aatggtcata
    48361 ggtctaattg ctggtggaga agaagcaatg actgtagctg tcgaaggggc cgaagattct
    48421 tatgagctag ggcaagaaga tctaatcaag ctgaatttga caaaaaatga tatagttatt
    48481 ggtatcgcag ctagcggtcg tacaccatat gtgatcggtg gattagatta cgcaaaatcg
    48541 atcggtgctg tcacaggaac gatttcttgc aataaacaag ccgaaatcag taaacacgcg
    48601 gatttcccaa tcgaagtcga tgcaggtcca gaatttttga caggctctac tcgattgaag
    48661 tcaggaacag cacaaaaact tatcttgaat atgatttcta ctatcagtat gattggtata
    48721 ggtaaagtat tcaataactt gatggtcgat gtcaaaccaa caaatgaaaa actaatcgaa
    48781 cgctcaaaac gaatcatcat gcaagcgaca gaagctgatt acgaaacagc tgaaaagtac
    48841 tttgaagaag cagatcaaaa tgttaagctg gctatcgtca tgattttgac aaatagtgat
    48901 aaagaggaag ctcaagcaaa attagctgaa gcaaacggat ttgtaaaagg gaccttatga
    48961 gggggaaaag accatggcaa aaaacgaact gactttggaa gaactggcaa aacaaattta
    49021 taaaggggca ggcggcatgg gcaatgtaga aagcattgtc cactgcatga ctcgtgtgcg
    49081 tatgaagatc atcaatgatg atcttgtaaa tgtctctgaa ttaaaagcga ttccaggagt
    49141 gcttggtgta gtagaagatg aacagcttca agtcatcatc ggacctggga aagtgaacaa
    49201 agtagcacag caaatggttg atttggcagg cgtgaaatta ggagaaaaac tttcttctgt
    49261 aagtacagct acagcaaatt catcagcatc tggaaaagac cgtgtaaacg aacgggcaca
    49321 agaaatgaaa gcggcacaaa aatcgaaaca aaaaccatct aaaatcaaat cggtattaaa
    49381 agacatttct aatatctttg tgccaatgat tcctgcattt gtcggtactg ggatcgtggc
    49441 agggattgcc gcagttttag caaacttagt cacagccgga acacttgatg cagcaacatg
    49501 gcaacaatat atcgatgtga tgaacatctt gaaaaacggg atgtttagct acctagttat
    49561 ctatacaggt atcaatgctg cacaagtttt tggtgcaaca ccaactcttg gtggagttat
    49621 cggggccgtt gtgatgctga cagggatgaa tccggaagca ccactgaaaa acttatttac
    49681 tggtgaagct cttgcagctg gacaaggtgg gatcctaggt gtcatcttcg ctgtttggct
    49741 attgtcaatc gttgagaaaa aactgcataa agtagtacct gatgcggtag atatcatcgt
    49801 tacaccaact ctttcattga ttgccatcgg tctgattgaa attttcttca tcatgccttt
    49861 agcaggtttc atttctgatg gcatggtcgg tgggatcaac tgggtacttg gcgttggtgg
    49921 agcatttgcc ggcttcgttc taggcacatt attcttgccg atggttatgt tcggactgca
    49981 tcaaattttg actccaatcc atgtgcaaat gattgatgaa actggtcgta cgctattgct
    50041 tccaattctt gcgatggctg gtgcaggaca agtgggtgca gcattggcat tatggattcg
    50101 ctgcaaaaaa gataaagaat taacagaaat gatcaaagga gctttaccag tcgggatcct
    50161 aggaatcggc gaaccattga tttacggagt aacattacct ctaggacgcc catttattac
    50221 tgcttgtatc ggtggaggta tcggcggggc agtgatcgga atgatcggaa atgtcggggc
    50281 tatcgctatt ggaccttcag gagccgcttt gatcccattg atctctgacg gtaaatggtt
    50341 aggctacatc cttggattat tggcagctta tgctggtggc ttcgttgcta cattcttctt
    50401 cggtattcct aaagaccgtt tagaaaaaga agaattagcg actgaaacag ccacagtaaa
    50461 tgaagtgcct gaaaaagtag tacctcaaac ttcagctgaa agtgtgacta tcctctcatc
    50521 agttgctgac ggaacagttg tttctttaga taaagcaagt gatccagttt ttgcacaaaa
    50581 gatgatgggt gaaggctact ttgtagaacc agaaaacgga catatctatt ctccagttac
    50641 tggggtagtc agctctgtct tcccaacgaa acacgctatt gggatcacaa cgccaaatgg
    50701 tttagaaatc ttgcttcata tgggaatcaa cacagttgac ttaggcggta cgccatttga
    50761 tgtgaaagta acagaagggc agcaagtgac acctgatacg cttgtagcag aagctgactt
    50821 agaagcgatc aaagcagcag gcaaagaaac atccatgatc gtcttggtaa ccaatatgga
    50881 tcgtgtaaaa aactttgtct tggaaaaaac aggtaaagca aaagcaaaag cacctgtgat
    50941 ggatgtcaaa gcttaatatg atagaaacaa tagaaacgaa agaagaaact gaaatctatt
    51001 tttcagcttc ttctttttta ttatttattg ttttctgcta aaatctgata aattcgtgtc
    51061 acatcagatg gtgttccccg taattcataa tctgccaaga aaggaaagcc aaatgtttgt
    51121 gcatattgtt tggctgttag acaatactga ttattgaagt ttttatttcc gctaccgact
    51181 acaccaagac aaagtttatg attgtcttca tgttccagat attcacgcat cgtttcggtc
    51241 aagatttctg tatcaccgtt atctaaacca tttccgccgt ctaaataagt aggaacgaaa
    51301 gtaaaaaatg gttctgtttc tttcgtaaaa tcactatttt catggatttc ttttaaagta
    51361 acttcaggat tgacaggatc ttctgcatgc attttttcag catattctgc tagatgtttg
    51421 gcaaaggcac gcgtatttcc tgaaatcgat atatataaaa tgttcatgga tcatcctcca
    51481 attctttcct tttagtatag catctttttt aagttttttg gtggtgtaat ttggaaaggg
    51541 actaggcgct ttgcttgtat aatgagaaaa aagccggtag aattatattt ctattaaagt
    51601 ttttattaat taagtaaaaa taaaaaaggt ttgttatact ttttgataaa agcggtttta
    51661 ttttggagga tgcttacatg gatacagagc aacagaaaaa aggaattggt ttaattccac
    51721 ttgctgcctt agtaattggt tcagcgattg gaggtggcgt atttggtatt atgacggaca
    51781 tctccggatc ggctggcggc ccagctattt tcagttgggt actcgtagga acagcaatgt
    51841 tgatgctctc cttgtcgatc aataatatca ataaaaaacg tccagattta aaaggcggga
    51901 tctttagtta cgcagaagaa ggttttggcc gtttcgctgg ttttatcagc ggttggggtt
    51961 actggctcac atgctggtta ggcaacgtag cttttgcaac attgttgatg agcgcattag
    52021 gatatttctt cccagtattt gaaggtgggc aaaatctggc atcgatcgtg atgagtacag
    52081 tctttttatg gggatatgca tggctagtga atcggggagt cgaaaatgct agtttcgtca
    52141 atgctatcgt cacattctgt aaattagtgc cattattttt atttattgta gtagctatca
    52201 gcacgttttc gatccaacaa ttcattgata acttttctag cgacattata ttgaatgcga
    52261 atggacagcc gatcccgttt tccagtcaag tattgagttg catcatggtg atgctttggg
    52321 tatttgttgg tattgaagga gcctctgtcg taggttcacg agcaaaaaaa caatcagatg
    52381 ttgggaaagc aacgattcta gggatattcg gcttgattat tatttacgtc ttggtttcca
    52441 tgcttccata tgggacaatg acgatgaatc agctgcaaac gatcaaggcg cagcctgcta
    52501 tgggctatgt gtttgaaatg atggttggta aatggggagc agtcgtgatc aacggcggtt
    52561 tgatcatttc tttagttggt gtttggctct catggacgat cttaccgatt gaaacaatgg
    52621 gaaacatggc tgaagacgga cttttgccta aagcatgggg aaaactcaac aaaaatgggg
    52681 caccaactta tgctatcgta ttgacgacgt tatgtacgaa tgtcttttta ctttcattat
    52741 tattcactga cagtgcttat aactttgctt acacacttgg tacagcagct attttcttca
    52801 cgtggctttt cttaggattg taccaaatga aactttctta tcagcggaaa gaatgggtcc
    52861 aatttttagt agggctttta gcaagtgcct ttcaagtatg ggctatgcta gtcgtagcct
    52921 ttaatgaagt aatgcttgtt cttgttcttt tcttgccagg catttatttt tacctcaaag
    52981 cacgtaagga gcaaaacgag cctgtacaac atatccactt agataagatt ttacttatag
    53041 gtgtgacgat ggtcggagtg attgcaatgg ttttatttgc cactgggatg cttcacgtat
    53101 agaagtataa aagaagtata aagagaggtt gtgagcctgt cgtttattca aaaacatact
    53161 aggaaaggag ctgtgacaca aatgttgtca cagctccttt ttcagaataa acggtgttta
    53221 agagacagcc aatcggcaat aaggctgaac gtctctttca cagcctcttt tttgatgagt
    53281 catcatacat tttttcgctt aaatacgaga tatcctaatg ctaagaacaa aacgatgtaa
    53341 gctaaattac cgaatagcat ttgattagta gaaagatgag tcaattgctt catgatctct
    53401 tcgtttccaa tttggttttg cagattcaac atactgatcg gattccattt gatccactcc
    53461 catttttgga tgagtgcaat aaggatacca gatatgattg acgaagcaaa ataaaagacg
    53521 attcctgctg agatggatgc gccggaacta ttgataaagc aagcaatcat caacactaaa
    53581 cttaaaatca gccataaacc gatataacta cccaaagtat aagtgaacaa gctttggatc
    53641 agtgtttgac cagtagaaac cttctccgtg aaggaaatcg aagggaaaag aatcagtttc
    53701 atcaacacag tgacaataat agctaaaagg tataaataaa cagaataaat aactaatgtg
    53761 atccatttgc ttactaatat ttctcctcga gaatactttc gataaaggag atttttcaat
    53821 gtaccatatt gtgcttccat cgaaatgatc gtgcttgcag ctgcaatcat gataaagaca
    53881 atccaagatg tggcactaaa aagctgagca gtcatttcaa ctggatcaat gatcgtcgtg
    53941 tattttttgg ctagaagtgc tgttcctatg agcaaaacaa ctaatagaac agataaaaca
    54001 gcagttgact ttttcttgat caatttaaat aattcttgtt ttacaagcaa tgtcattttt
    54061 cttcttcccc ctttactgaa tccagaattt ccaatagaga agattccaaa tcattcgtca
    54121 gatgctggac gtctgtcaca tggatatttt gtgcagacaa tagctggatc acggttccta
    54181 ataattcttc gttttctttt aaaagcttga ttttatcagg atcttccaaa gtatatccag
    54241 cttctgtcaa gatcgttcga gcagctagat catttgttgt agagataacg atgaaatctt
    54301 gcgctgtttg gttcaattca gccattgtac attgtttgat gatctttcct tgattgatca
    54361 ggatcagctc atcgaccatt ttttcaagct cgctgagtaa gtgactggag atcaagaaag
    54421 aggtgccctg agtagccaag tcggtaatga tttttcgcac atctcttgtt gcttgtggat
    54481 ccaaaccgtt catcggctca tctaaaataa cgatagacgg atggttcagt aaggcaagag
    54541 caattcccaa tttttgtttc attcccaacg agtaggattt cgcttttttg tgaatgtact
    54601 tttccatctt gagctgattc acgatagtgt cgatagcaga tacatcgttg gtttcagaga
    54661 ataacttcag atgatcatag cctgttaaga atggatagat acccgggtat tcgatcaatg
    54721 ctcctacttg ttccaaccct ttatgactag agggagaaat ctcattttca ccaatacaga
    54781 ttgttccatc tgagtaatgg atcagtccta aaatagcttt catgatcgtt gtttttccag
    54841 cgccattagg acctactaat ccaacgattt tgccagggga taagctaaaa ctgacatcgc
    54901 tgagggcttg tttgtgacca aagttcttgg ataattcttg gaccgaaaga cttttcaaaa
    54961 atattcactc cttttctata gatagtacaa gcatacacct gatgagtgga gaagtcgcta
    55021 ggtcattgga cttatttctt attttttcat aaaaaaattg aatattaaat aaaaaggctt
    55081 attttttctg atcagtactt attccaagtg tctagtggcc gctagtaatc ttgatgccaa
    55141 tgatactgat gagtagcatc cccacaaaga accaagtgga aagattcagc ttatcatgaa
    55201 acaagaccac accaattatg accgaaccaa ctgcgccaat ccctgtccag ataggataag
    55261 agagactcat cggcaaactt ttgattgcct ttgacaaaaa gtaaaaacta gcaatcatgc
    55321 caatcactgt gtaaaaagaa taattgattt tagaaaaacc atcactccat ttcatcatcg
    55381 ttgaccagaa tacttccaaa attcctgcta tgattaattc catccatgcc atacttacat
    55441 tcctccttaa ctataaaaaa atacacaaaa agaggaaaat catcccttca aagacctaat
    55501 ggaatgatca tcctctttat cctacccggt ggcagattgt atatcttttt ctgtagtata
    55561 gcacacctat ccagatatcg gcaatctcat actttttagt aaagtgtacc aagcactgac
    55621 ggatatatcg ttagcggaaa agaagatgaa tgctttgcaa tttcatagaa agaagttgtt
    55681 atttattaat catttaaaaa aaacagtaac tagaccttat ttctatatat tatcgggcaa
    55741 taaattaaaa aaagctttac cttacaataa gtaagataat ttttgtgata aaaaaataac
    55801 gaaaaatatt taaaaatagt gtctatttcg tgagaaaaca actatatttt ttatgtgttt
    55861 tgctttataa ttaaaaaaat attgtcattt tatggaggaa aatatatgga aaattatctt
    55921 agccttttag gcgtcttgat tgttattctc gggtttgcat ttaaattaga ctcgatattg
    55981 atcgtaatgg ttgctttagt tgttacagct ttgactggcg gtcttggcat tcaaggaatg
    56041 ttagaacttt taggtacaag ttttgtaaac aatcgcggga tggcgatttt tatcatcatt
    56101 atgttggcaa caggaacgct tgaaagaaat ggtctgaaag aatcagcggc ttccttgatc
    56161 aagcgattca agaaagtatc tgctggtatg attgtagata tttatgggat ctttcgaatg
    56221 atctttgcgg cttttaacgt gagttttggt ggagttgctg gatttgttcg tccaatcatt
    56281 ttaccgatgg cattggggac catcgaatca aaagggttag aagtccatcc taagcatgaa
    56341 gaagaactaa aaggtatagc ttcagctatg gaaaatgtct gctggttctt tggccaagta
    56401 ctattcatcg gaggagcagg ggcgttgcta gtccaatcaa cactgaaaga tctaggatat
    56461 gacgtaacat tgggacagct tgctttagtg gaagttccag tggctatcac ggctttagtt
    56521 gtggcaagtg tctacttcta tcttaaagaa aagaaattag cgaagaaata ttatccagga
    56581 aaggggcaat aagatggact tttttacaaa tcctgatatt ttattaggcg ataaattact
    56641 ggagatcctt tatatcgtga tgggactcgt attgatttac actggagtaa gaaatttaac
    56701 agataaaacc aatcctcatc gttatgggtc agcctttttc tggacggtct taggggtagt
    56761 tattgcagga ggaagatggc ttccggcaat cgtgaacggt ggcttgatct ttgcgatgac
    56821 cataccagca attgctaaga aagtcagcaa aggagaaagc cggcttccat caaaagaata
    56881 tatggaaaaa atgtcagaca aactaggaat gaaaattttt attccagctt tgagtatcgg
    56941 tgtctttgcg atcttgtttg ctctatttac caatttaggt gctttagtcg gcgtgggcgt
    57001 cggtgttttt gcagccatgc taatcttgat gttcttttct cgagataatc gaccaagtac
    57061 atttttagat gatgcagcag atatgctggg cacagtaggg ccgttaagca tgttgccaat
    57121 gcttttagct tctctaggtg cggtatttac aagcgctgga gttgggacag tgatttccaa
    57181 tgcagtaggt acgatcgttc cagaaggaaa tgtgaacatc ggcatcatca tttacgctct
    57241 tggcatggtg atatttacgg taatcatggg gaatgcattt gcggccatca ccgtgatgac
    57301 ggttgggatc ggtgcaccat tcgtatttag tcatggagcg aatccagcgt taataggaat
    57361 ggttgcattg acttgcggtt tttgtgggac cctgctgaca ccgatggctg ctaacttcaa
    57421 tatcgtgcct gtagcgatgc tggagatgaa agacaaatac ggagtgatca aaaaccagtt
    57481 gtttattgcg ttatttatgc tagttttcca aattgcttat atgattctat ttaaataata
    57541 gaagagaggg gttgtgacag aataattgtc gcaacctctt ctaacattgt agtggaacac
    57601 tattttttta taagaatata ttaagtttga tctgttatgt tatttaaact attgtttcct
    57661 ttatttacgc tgttcaagga aaatggtatg ataaaagata accatatgtt aaatcaatcg
    57721 gacggaggaa caacagatga aagtactagt aacaggtttt gatccatttg gtggggacaa
    57781 agtgaatcca gcctatgaag cagtaaaaaa aatgccagat gaaatcagcg gtgctgaaat
    57841 catcaaagtg gaagtaccga ccgtattcga aaaaagcagt caagtagtca aagaagccat
    57901 ccaacagcac caaccagatg tcgtgatctg tgtcggacaa gcaggtgggc ggtcagcagt
    57961 ctcgttcgaa cgtgtcgcaa tcaatctagc agaagcaaga atccctgata atgaaggaaa
    58021 tcagccattc gatacggctt tagaagaaaa cggtccagcc gcttacttta cctcattgcc
    58081 aatcaaagca atgacaaaaa atgtacaaga tcatggtcta ccagcgtaca tctcttatac
    58141 agcaggaaca tttgtctgca atgatatcat gtatcgtttg cttcatttga tcgaaacaca
    58201 attcccggca attcgaggtg ggttcatcca cgtgccattc tcacccgatc aagtgatcga
    58261 tcgtccagtg ggtacgcctt ctatgtcttt agaagacatt gcttcttctt tgacttatgc
    58321 agttgaagca gcagtagaaa ataaagaaga tatttccgga aatgccggaa caactcacta
    58381 atttagcaag aaacactggc tggttgctaa cgttatgtta gtagccagct ttttgtaatt
    58441 agaaaggaaa gaaacagccg tggaagaatg gcaatccgtc tttgaagaat ggtttccaaa
    58501 agaaatcagc aaaagctatc ccatcaaaat ttctaaacaa tacacaagca gtcaacgttg
    58561 ggagatctat gcaaaattaa cgaaaaaaca gcgggaacta gtcgataagc accgccgata
    58621 tctaatcagc tctcgtttta tggaagaaca ttatttagct gcaacagatt gggtgttttc
    58681 tgacttcaag atcaatccgt tttttcgaac aaaacgaagc cagcaaaaat tgtattgtga
    58741 gtgtggtaga gaattaaaag tgcaatacat cgttaaatcg ccaaaaaccg gaaagatcct
    58801 aaaactaggc atcaaccact ttgccgatca cctgcatgtc tctccgacag tggcagcttc
    58861 gatccatcag ggaatgacaa aagttgatct ggccttagac gaattattat ggcttaagca
    58921 gaagaatatc gattttcctg aaggactatg gcaaaaatac tgttttgtct tgtaccaaaa
    58981 tcgtcggatg aaacagccat atttaccgga tataaaatta gctcagcggc tcgcagaatt
    59041 tcgccaagta gaaatgccga tttacatagc ggattatcaa gcattggaaa atgaaatcaa
    59101 gaaaataagt gaacacatca atgggcagtc aaaaaagcga cagataaaaa aagaactatt
    59161 tgacgacttt gcagaagaat tagtaaagga tgtagaagaa ttcttgatca actatcgcgc
    59221 atttttacgt aaagattggc aatccatcgt atatgaagaa gtgccagtac atcctaatgc
    59281 ttattttgaa acatttattt ccgtgttgag aaagacaaaa cgtcaacgaa caccagaagt
    59341 cactgctcag atggaatatt ttgcgaaaaa ccaacgattc atccaaccaa aaatctatct
    59401 atttatttgg aagcaatatt gtcgctatgg ttttacagaa gggttcttcg acagcattcc
    59461 tcggatagtc agaaatggtt ttttaaaagt attgcgtaaa gaacgagaag ctatacaatc
    59521 tgctgataag aaagaccgta cagtctcaaa agaaaaatgg caactagtag tgaaagatat
    59581 ccaatctgga aatgtgcaag agacaatcga caaatggaaa ggaaaacatt atcgttttac
    59641 tgaagctcag aaacaagcgt tggaatatta tcagaagtta gaagaatcac tgcgtttcaa
    59701 tgatgaggca agaaagtatc tgaaggaatt actataaaaa atagaggctg ggacagaagc
    59761 gtttaactcc aagaaataag aagaaattca cgaaaattgc ttttcaaatt tttgtgaatt
    59821 tcagcttatt tccgaaggag ttgcttctga ttccgccgtt tatacgtgtt tagagcgtga
    59881 gacaaaagtg ttctttactt ttgtcccacg ctcagttttt ttatgcgtgt tgttgtgcgt
    59941 tgtagtattc ttcttccatt ttttgcatac gttcacgttc tttttggtta taccaaggtg
    60001 aaacggtaaa tgcaaggaca tcattcagca agtaaacaga gctgttcacg aacatcgcca
    60061 aacttgcaga tccatgcgta aatgaaatat accaaaggac catttgagca agccctgaag
    60121 ctaaccacca gtaatattgg ttgttgtaac gcaagaaact gatgatacca gcagtcaagc
    60181 taatagaaaa agcaatggca tctacccacg gccgaggatc atctgtataa cgaccaatca
    60241 ggaatcctga aacaagatag acaaggaaag tactgatgat tgcgataaac cagctttttc
    60301 cagtgaattt tcggattttt gaagccatat tatgattcca ttctttactt aacaatacgg
    60361 ggatatccaa tgtgatcata taagcaatct gttcaccgat acttaaatag ttctttgctg
    60421 aaaacccaac gataataaaa caaatcgcag acaaaatacc taaaataccg ttgactgatt
    60481 ttgccgcatt gatggataaa atacacaata caccaagcgt ggtaccgata aatgtaataa
    60541 cggacagcca agtgatggga ctattgacaa gagtcatgac ctggcagcca agactgaaaa
    60601 agaaaagata ataattttgt tgcggccagc cttttagctg atgagctaaa aactgaaaat
    60661 atgacttcat gtatgaggac tccttaagac actaaatgta gtgtgttctg tatttactaa
    60721 cacaatctat tgtatgcttt tcttgaaaaa aaactagtct ttcaattata aataatttgt
    60781 gttatgataa ttttctgttt gttttcttga aaatataatt tcttactttc atttattata
    60841 ttgggaacag gaagagtgtc ttggaggacc tactttctgt tttttttgcc ttttttctta
    60901 taaaaaggaa aaacagctat ttttttcaat ctgacacatt tacaatggaa ctggagatta
    60961 atatagatcc gagcttaaac gaagaatagg agtgctcata tgaaccttac aacgatccat
    61021 catatagcca tcattgtttc agattatcag aaatcacgtg atttttatgt taataaatta
    61081 ggatttgaag taatcagaga aaattaccgc aaagaacgag atgactataa attagatctg
    61141 aaactaggaa gtgctgaatt ggagattttt ggaattgcta atgcgccaca acgaccaaac
    61201 tatccagaag cttgtggctt gcgtcatctt gctttcaaag tagacgatat tgaagaaaca
    61261 attgcagaat tgcagtcctt agggatcgaa tcagaaccga ttagagaaga tacctttaca
    61321 ggaaaaaaaa tgacgttttt ctttgatcca gacggattgc cattagaact gcacgaataa
    61381 agataacaaa attgtcactt ttttttcgtt tcattcgatt tttcattcgt tcatttcttg
    61441 atattcttta aaaaaaagaa acaggagaat gaagaatgaa aaaaatgatg tttggtgttg
    61501 ctgccctcat cgtaataggg attaccggta tgacgggact gctcaatgcc gcttttcaca
    61561 tggatgatat tagtgaccag gaatatcgga tctcattgaa aaaagcagca gcaatcgtaa
    61621 aaaacgaaac aaaagaacat aacatatctg ctattcattt tgcagttgaa aaagatttgg
    61681 agaatcctac gaatgcaaca tatgaatacc agtttttttg tgaaggacaa gtcatagcag
    61741 tcaatccatc cactgggaaa acaaccgtac gaaatgacac ggaaaaggcc gcagaacatc
    61801 tttttgatat agaaaaaacg gaagagttca aacaaccgca agcagcgatg aaggaagcag
    61861 tcagctacta cgggaaaaaa gcaaaagtga aagagtggac acttctagtc aaagatgaac
    61921 gtccttatta taaagtccga ttaatagatg gagaacagga ccgtcaggta tggatatctg
    61981 catagaagaa ggatattgct tctctttttt acaatataag agtaagctaa gagagtaaag
    62041 atgaaaggaa gttgagataa catggaaaaa atgaaatgcc ctaattgcgg aaaaaaattt
    62101 gcatacgaag aagtcaataa tgtagtcgaa cataatgata aagaaatgcc agtcgtctgc
    62161 ccttattgcc gaactgaagc ggcaagaatc gttactcatg gttatttcat tactgaaaag
    62221 attgaagatt ttttaaaata acgattttgc gatttctttt tctgcatgtt agaatggaga
    62281 aaaggagagg aaactatgat tcgttttgcg agaaaagaag atgctccaga aatcgcccca
    62341 ttgatcttag tgattctaaa agatatggaa ctgccatttt tgaagcagac aggggaacaa
    62401 aagactttag agattttaga agaagcaatc actgatccaa attatcgtta cagttattca
    62461 cgaggtatcg tagaagaaat agacggtaaa gttgcaggag ttgcttacgg ttatacagat
    62521 gaagaagaat caatcatcga tcagccactg gtacctatct tagagaaata tcagctagat
    62581 gcgtctacac gtttgttcat tgataaagaa acgtttccca acgaatggta tttggactct
    62641 atcagtgtat cagaagattt tagaggacaa ggcatcggtt cgcgattatt agaagcatta
    62701 cctaaattgg cgaagaaagc taatcgctcc gtcatcggtc taagtgtgga tgaaaaaaat
    62761 ccaaaggcta aaaaattata cgaacgccat gacttcaaag ttgtcggtca gcggatgatc
    62821 agtggtcatc tttatgatca tatgcaaaag aatatctaaa aagatgtacc tgaaatgcgt
    62881 atttccgcat ttcaggtaca tctttttaac ttaagttaag tcggtaagaa acaggcttat
    62941 cgcgactttt gtctttacgg tagagccagt aatctaaaac cgcagcaata caaatacaaa
    63001 ctggagcatc ttcatcattt gagacatcca atacatagta atctcctgaa aaaagtgttg
    63061 ctttatccat tttcatgatt tgctgattga aatgatggat ttgatattga tgattataga
    63121 tatcaccttg gactgtccag tgaagtctac gaatataata aaagtcaccg ggccaattga
    63181 atattttctg catggttccc accttttcga actctttata cagctcaaaa cgcgtaccga
    63241 atgtcaggct tatctgcttg atgtgagcaa cgagatcgcc attcatggca taaagagaca
    63301 atgcatcgcc ctttgttccc cagcgaccaa ccatcaaaaa cagggaatgt cctttttcgt
    63361 ttttgacaat tgtccgagtt gcacgtccaa gctgcttttc ctgaatgaaa aattctgaca
    63421 tctatatccc tcttttctag ataaaactac aagtcttctg caatcgcgcg caatatggct
    63481 tttcctacgc cagattcaag gtttgtgtcc gttgtatatt tggcataatc ttttacttcg
    63541 atttctgcat ttcccattgc gaaactgact cctgctactt gcagcatgct gacatcattg
    63601 aagttatcgc caatcgtcat gacttcttct aaagaaatcc cacgctcgtg agcgacatgg
    63661 gcaacagcaa ttcctttttg agcgttttta tggttcactt cgatattgtt ttgaccagag
    63721 gaagtaacgg ctagatcgcc taaaagagaa atctgctttc caactggacc taatacagct
    63781 ggcccttctg tatgaaaaca aataattttt aaaacttcca gattcttttc ttccaaaata
    63841 gcacgaatac tgtccacgta tgtgatgtgc aataactcca gcttagctga ggccatggca
    63901 atcgccattt tatacgtaag gtgaggtaaa tgggtagcca ccatggaagc aaaactttca
    63961 attcgtttcg cttgattttc agaataaaga ccatcgttgg ttgctacttc atagtaaata
    64021 ccgttagcat ctagaatatc taaaacagag attgcttgat tatttggaat cgacacggta
    64081 aacaatgaat ttccttcttt atcaaatacc tgtgccccgt tcaaagtgat catcgcacaa
    64141 tcgattccta cctcatctaa agctgcgcga gcttcttgat agtttcgacc agtagcgacc
    64201 atgaactcaa caccggcatc atttgcaaaa cgtatcgcct cggcattttc aggagaaaca
    64261 ctcatatggg catctaataa tgtgccgtcc atatctgatg caattaattt gatcataatg
    64321 attatcgttc ctttctgctt cttgttccta gtttaccata ttaatcaagt gctgagggtg
    64381 aagattgtcc cttgtgattg aagaaaatgt gagttattct ttttattagg aggtggagtt
    64441 tttgaaagaa atcattcata acagttggca agaagttttg tcctcagaat tttcaaaaga
    64501 ttattatctg catctaagag aatttttaaa aaaagaatat gcgtcccaaa agatccatcc
    64561 agatatgtat catatctatg aagcattgga attgactcct tatgaagaag ttaaagtggt
    64621 gattttagga caagatcctt atcatggaga aaatcaagct catggcttat ctttttctgt
    64681 gcaacctggc gtaaagatcc cgccatcgtt aagaaatatc tataaagagc tttatgatga
    64741 tcttggaatc gcacctgttc aacatggaaa tctagtgtca tgggctaaac agggagtcct
    64801 tttactcaac accgttctta ccgttcggga agggcaagcg tattctcatc gcgggaaagg
    64861 ttgggaaaga ttaacagaca cgatcattga aaaattaaat gaacgggaaa aaccaatcgt
    64921 gtttatcttg tggggaaaac cggcacaaga aaaaatcaaa atgatcgata agagtcgcca
    64981 tatcatcata acatctccac atcccagtcc gttatctgca agtcgtggct tttttggttc
    65041 caaacccttc tcgaaaacga atgatgcgct ccttgcgtta ggagaagaac cgattgattg
    65101 gcaactgcct gaaacagtgt aataaaacag cgctttcctg ttatagtgca gttcgctttg
    65161 tatgtgaaaa acttaatttt tccagcattt ttgactgata cagataaaat aaactgttct
    65221 ttttttctgg aatagtgtat gataatacat ataaaaacgt ctggaggtca tcttgtggaa
    65281 ttgtttgata gtttaagatt taaaattgtt cgccgtggga tcaaaatcgt tttcccggaa
    65341 gctactgatg ctcgtatttt aggcgcagct gcccggttga aagcagaaga acttgtggaa
    65401 cccattttag tagggaacga agaagaaata agaaatgcag cgcatgccag aggaatcaaa
    65461 acatctagct ttacgatcat cagccctgat aattacgata agtgggatga aatggtggat
    65521 acttttgttg aacgtagaaa tggaaaggca tccaaagaac aagcagaaaa gattttaaaa
    65581 gatgtgaatt actttggtac gatgcttacg tatatgggga ttgcagacgg aatggtctct
    65641 ggagcgatcc attcgactgg cgacactgtt cgcccagcac ttcagatcat caaaacaaag
    65701 cctggcatca gtcggacaag cggagcgatg atcatggtac gtggaagaga tcaagaaaaa
    65761 tatgtctttt cggattgtgc gatcaacgtg aatccaactg cgcaagaatt ggctgaaatc
    65821 gccgtagatt ctgcaaaaac agcagaattg ttcgatatcg aaccaaaagt agcaatgatg
    65881 agcttctcga caaaaggctc agcgaaatcg ccagaagtag acaaagtagt tgaagcaact
    65941 aaaatcgcta aaaaactagc gccacaatac atgatcgacg gcgaattgca atttgatgct
    66001 tcttatgttc cttcagttgc acaactgaaa gcgccagatt cagaagtagc tggtcaagca
    66061 acagtctttg tatttccaga attgcagtca ggaaatatcg gctataaaat tgctcagcgt
    66121 ttcggtaact ttgaagcaat cggaccaatt ctacaaggct tgaacaaacc ggtttctgat
    66181 ttgtctcgtg gagcaaatga agaagatgta tataaattat cgattatcac agcagcacaa
    66241 acattgattg attaacaaat acacgcgaac tagccaaaaa cagcagctgc tgttcttgac
    66301 tagttcattt tttctttaaa aaaacttcat tacgcgatat actggcaaaa agaaagaaga
    66361 gcgggagaag atgaaaaatg atcgaactat caggattaac aatgacagaa caattcgcag
    66421 aggcaattgg tcattccgca atgccaggtg acaatctgat attgactggt gatctaggtg
    66481 cgggtaaaac aacgttgacg aaaggaattg cccaaggatt agggataacg caaatgatca
    66541 aaagtccgac atacacgatt attagagaat atagtcaagg tcggattccg ttgtatcata
    66601 tggatattta tcgggtagca gctagcggag cagatttagg gttagatgaa tattttgaag
    66661 gtgatgggct ttccgttgtt gaatggggaa atctattaga agaagcactg ccggaggatt
    66721 atttggaact gattttagaa aaaagtgata cagatttaga aaagcggtat gtgaaactaa
    66781 tctcatacgg ggagcaaagt gaaaccttca aacagcgaat tctagaaaaa tgggctgaaa
    66841 atcatgagtg aagaagttgg gatcatcttg cgggaagcag tgccaggaga tgcaaaagac
    66901 attcttttga tgatgggaca agttaataag gagacagaat ttttagtttt ggatgaagca
    66961 gaactcctgt taccaccaga aaccttagaa gaagagttgg attatatata cgaaagcaat
    67021 aataatttat tgctgcttgc aatctatgaa gggacgatta ttggaacagc atctgtcaag
    67081 gcagacagtc aatttcgttt gtctcacgtg ggagaagtcg gtatcagtat tctgcaggaa
    67141 tattggggaa tgggactagg cactcttatg ctagaagaaa tcatcagttg ggcgaaggaa
    67201 atgggtgtac tttttcgatt ggagcttgac gttcaagttc gtaatgaaag agccgtacac
    67261 ctttaccgaa aaatgggctt ccaaatcgaa gcggtcatgc ctagaggggc aagaacagat
    67321 ctaggtgaat ttttagatgt atataaaatg agttatttga ttgaatagaa gaaataggaa
    67381 aagagtgaga aactgtcatc cttttgagaa ggcagaaaaa cacacgaagg gactgtgaca
    67441 aaagtcgtgt cgcagtccat tttttttgaa taaacggtgt tcaatcagct gaccttcggt
    67501 attagctcaa aaaacggcaa aatatgagga actattttgc cgtttgtttc tctaatactc
    67561 aggtcaaaac gctgatttca caacctcttg gtaaacggtg tccaaaagct tgcatctgcg
    67621 gtaataagtc caaccaaatg aaaaatatga gaagctattt ttgtttggct gttcttatta
    67681 cttgatgcag aacagctttt tcacaacctc tccaataaac ggtgttctcc tgccagctcc
    67741 tcggtcacca gtcacaatat ttaataaaca tgagaagcta ttttttgaaa ttgctccttg
    67801 tgactcggag tgaaacggca ggctcacgac ctctttgtgt gtttcataag tattctttta
    67861 ctttatagtc acaagttttt tcaatggatc tgtcccgaat tttttttctt gataaagcag
    67921 gatttccgca catgcacgac tatcttccaa tgcatcatgg tgatgatcta attggatgtt
    67981 caaattttcg cacacagtgt tcaatcgatg atttggaaat tcaggaaaca ggcgacggct
    68041 acttttcact gtacataaag aaagataatt aggctgttca agatgataat aatctaagca
    68101 tcccgctaag acctttgtat caaaagcagc attatgtgca acgactaaac gatttttttg
    68161 aaaatatggt tggatagttt gccagacttc tgggaatttc ggcgcattgc gtacgtcttc
    68221 ttgatggatt ccgtgaattt ggacatttcg ccagaaaaaa ggagtctcag gctgtatcaa
    68281 tgtatagaat tcatcaacga tctggctatt ttttaccatg actagcgcaa gagaacaagc
    68341 actataaggc tgatgattgg cagtttcaaa atccatggca acaaaattca tttctgtaaa
    68401 acctccttgg gaaattactg taacatactt ttgaaaaaag caaataaatt taacaaaaaa
    68461 catagaattt tttatgtaat atttgttgag aaaagctagt ttttgaactc attccttgtg
    68521 atttggagtt agatggggat catgacttct cggtattaag tcaaatttat gcaaagttgt
    68581 gaggaaccat tttcaaaaaa atttcttacg actcgaaagt aggcgacttt ttcacaactt
    68641 ctccactaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa tagcaaaata
    68701 tgaggaacta ttttgccatt tgtttctcta atactcaggt caggacgctg atttcacaac
    68761 ttctcaataa acggtgttca aaagcttgca tctgcggtaa taagtccaac caaacgaaaa
    68821 atatgaggaa ctatttttgt ttggctgttc ttattacttg atgcagtgca gctttttcac
    68881 aacctcttaa taaacggtgt tcaaaagctt gcatctgcgg taataagccc aaccaaacga
    68941 aaaatatgag gaactatttt tgtttggctg ttcttattac ttgatgcagt gcagcttttt
    69001 cacaacctct taataaacgg tgttcaaaag cttgtatctg cggtaataag cccaaccaaa
    69061 cgaaaaatat gaggaactat ttttgtttgg ctgttcttat tacttgatgc aggacagctt
    69121 tttcacaatc tctcgataaa cagtgttcaa aagccagttt ctcggtatta tgccaaattt
    69181 atgcaaaatg tggaaagcca ttttcacaaa atttttctta atactcgaaa ctaaacgact
    69241 ttttcacaac ctctttattt caaatctaat tctactggac aatgatcact gcccataatg
    69301 tccgtatgga tctttgcatc agctagtcct ggctctaaat ctttgctcgt caaaaagtaa
    69361 tcgatccgcc aaccagcatt gtttttcctt gaattgaatc ggtagttcca ccaagagtag
    69421 acaccttcct gggttggata aaaataacgg aatgtatcga taaacccgtt atccaacagt
    69481 cgagaaagag ccgctctttc ttctggagtg aatccagcat ttttttgatt cgttttccag
    69541 tttttcaaat caatcttttg atgagcgaca tttagatccc cacacacgat gactggtttt
    69601 tgttttttta gattttccaa ataattataa aaggcttctt cccattctaa tcggtaatct
    69661 aaacgtttta attcgctttg agaatttggc gtgtaacagg ttacaagaaa gaaatcggaa
    69721 tattccaaag taatcagacg gccttctgta tcatggatat cgatacccat tccgtaagtg
    69781 gcattcaatg ctggtttttt agcaaaaatc gctgtgccgg aatagccttt tcgttccgcg
    69841 taattccaat attgataata acctggaaga tctaaatcaa tttgtcctgc ttggagtttc
    69901 gtttcctgca gacagaaaaa atcggcgtct aactcatgaa aaacttctaa aaaattttta
    69961 tttacgatgg cacgaagccc gtttacgttc caagagataa atttcaataa aagcacactc
    70021 cttcgaatta atttccttac ctttgcgata ctattgtacc tcattttagt aaaaaatggt
    70081 gtaagatgaa agtaactaaa tgatccagga ggaaagtaga tgaagattgg tgtaatcggt
    70141 gtagggaata tcgcagaaaa agcatattta ccaacttatg cgaaaaaaca aggaacactt
    70201 gatttttatt ttgcaacaag aaataaagaa acaaaagaaa gaattcaaaa agagtacggg
    70261 ttccaacatc tatatgaaac gatcgatgag cttttagaag aaaaaatcga agcatgtatg
    70321 atccacgcag caacaaaagt acattatgaa ttagccaaaa aatgtctaga aaatggtatc
    70381 catgtttata ttgacaaacc actgagtgtc catctaaatg aagttcgtga attgcaagaa
    70441 ttagctgata agaaccaaat aattttgatg atcggtttca accggcgctt tgctcctatg
    70501 gtagaagaat tgaaacgtat cccagataag cggttgatcc acctgcaaaa aaatcgaata
    70561 gcggcaaaag aaacgacaga gttcgtcctt tatgatttgt ttttacatct tattgataca
    70621 gctgtgtatt tattagatga acccattttg agaacaaaat accagataag agaaacaaag
    70681 gaatttttgg aatatgccat tttacaatta gaaacacaaa atcaaacggc tattttaacg
    70741 atggatttag caagtggcgc taatatagaa aaatatcaag taacaagtaa aaatggtact
    70801 tatgaagtaa acgatctgac ccagcttgtc attcaagagg ctgatggaaa aaaagtgaaa
    70861 gaatttggcg actggacaaa cacgctagtc aaacgcgggt ttgagccaat ggtagaggcc
    70921 ttcttttctc agatccgttt aggtaaatcg gaaacagaat tgcttaaaca aaaagaagtt
    70981 gtaagaagtc atgaattatg tgaggaagtg cttaaagatc actaccgtca tcaactttga
    71041 acaagactgg caagattgta ttaagcgtga tagaataatg aggacgtttg agaaaagaaa
    71101 ggaaaatcct cagtgaataa agaagaaata gttgaaagct tagccacgat tactatattg
    71161 tttgatgaac cattgatgaa ctatacgttt acgaaaacag gtggaccagc tgatgtcctt
    71221 gcattcccga aaaaacaaga agaagtaaag caaatcatcg attattgccg aatccatgat
    71281 ataccttgga tggtattagg aaatgcaagc aatctgatcg ttcaagatgg cgggattcgc
    71341 ggtgtagtca tcatgctgac tgaaatgaaa cagattcatg tcaaaggaac gatggtcatt
    71401 gcggaagcag gtgcctcact gattgatacg acttatgcag ctttggcaga atcattgact
    71461 ggatttgaat ttgcttgcgg catcccggga agtgtcggtg gagcggttta tatgaatgca
    71521 ggtgcgtatg gcggagagat caaagatgtt tttgctgaag tggatctttt gcttgaagat
    71581 ggaacattga aaactttgac aaaagacgaa atggtatttt cttatcgtca cagtaaagtc
    71641 caagaattac gtgctatcgt attggaagct cgattcagtt tgcaaacagg ggattatgaa
    71701 gcaatcaaag caagaatgga tgagttgacc gaattgcgtc aatcaaaaca gccattagaa
    71761 tacccttcat gcggaagtgt ctttaagcgc ccagtaggac actatacagg tcagctgatc
    71821 caacaagcag gattgcaagg cctgaaatgg ggcggtgcac aagtctctga aaaacatgct
    71881 ggattcatcg tcaatatcga tcatgcgaca gcgacagatt atattgaatt gattgcgcac
    71941 atccaagaag taatcaaaga aaaatttgat gtgtctttag aaacagaagt gcggatcatt
    72001 ggagaaaaac aagcaatatc agaaacaagc agaagtttag ccaattaata agtaatggaa
    72061 cttaaaataa gtaaaagagc aggacggtgg ccgtcctgct cttttttgtg gttgtttacg
    72121 caaatggcgc aaatcttgta cattttaaca caataaaaca aaaaatatag aatatttcct
    72181 cataaatttt tccatttttt gcaatattct caaaaaaaag ataaaaaatg agaataactg
    72241 atgaaaaaac aagcataatc catcataaaa tcagtatagg tgaaaagatg aactttcatc
    72301 tgcatatcaa gtaattggta tgaggcattt tttgaggtgt aggatatgaa gttggattac
    72361 cttatagaag aggaagatag agaaaaactg ctgctagcta gatatttaga atttgagaaa
    72421 aagaattatg tagtgctgcc taagttatat gaaacattag gtttgtctaa atttaaaaca
    72481 agaaattata tcatagaatt aaataaagaa tttactgaat tcaaaacaaa gccagagatc
    72541 caaatttcag aagaagcaga gatccatgtc gaacgtctga atttgacgga tatcaaaaat
    72601 ttaagaagaa tctactttca acgatcaaaa attgctcgac tgttaacgga gatcgtggat
    72661 tcaaaagtaa cgatcgaaaa atttgcagag aatcattttt taagcatatc aagagcctat
    72721 gtaaaaagaa gagaattagt ctcttttctt gaacagcatc aaataaagtt aaagaaaaac
    72781 atattagttg gggaagaaat aaacatacgg aacgtattat ttggggtata tttcggggtc
    72841 tttaatggtt tttctatgcc atttagagaa agcgtcatga atgaagcaaa gagtatcgtg
    72901 caatattttg aatatctgtt caatttaaaa ctgtctgcaa caaaacgaat caaattcaat
    72961 ttgctaacca ctattagttt gttgagggcc tatgggaatc aaccaatcgg aaaacgtttc
    73021 ttttcagatg aatactttaa gagctcagaa ggaatgaaga tcatcaagga tttatcacat
    73081 atttcttttc ttcatatccc taaagagcag atcattgatg agatttctta tatcatgttg
    73141 tttttgatca cggaagaaga atttgaatta gatggcgcat ttggacttgc ggaagctcat
    73201 tttcatgata tccacatgat tagtcaaaaa atgtcagtgg aattgattga tcaattacca
    73261 ttttcgattg aaatctctga agaagaaaga ctatatatac gaaaagaact tcgtatgggt
    73321 atgatgcatg tcaatcgaaa aaattggata ttcgactttg agatgactag ctttacgtct
    73381 aaaaagcagc tacagttttt cttcgagacg tatccaatat tcagtacagc aatccaaaag
    73441 gtcatacaag agaataaacg ctatttcttg ggaaaagatg aacgtttcat gattcgctta
    73501 ttttatgatt atctcttttt attggtcggt atttgtccgg tatcatattt tgaagcaccg
    73561 attcatgtct gtattgattt ttcgcaagga tctgcttata ccgattacat tatttctcaa
    73621 gtgaaaggat ttaaaaatta caatattgtg attgagagtc gaatcacttc tcgtacgaat
    73681 atttatttat ctgattgttt acttgaaaaa tttaataaag aacagataat ttggaaaaat
    73741 ccaccgactc cagatgactg ggaatatttt ggaaatacca tcatcagaga aaaaaataga
    73801 catttaactg aaaaaatcag ctagtattta tggatggcag ctttgtctca catatttatt
    73861 agctgattta tgtttttttc aaagaaaagg taactagata taactagata taactagata
    73921 tattacttaa caaaattaaa aagtacataa gttattattc cgattttctt gaaacaataa
    73981 aaaatcaaaa aaaatactct cctaatagtc catcttcttt tgattttatt tatataatga
    74041 aatcatctta tgaaaattac tacttcatgt ttagacaaga gtagggagga ctaagaataa
    74101 atgtgttcaa aaagacgttc aaaacaacta aataaacacg cttgtcgttt agataaggaa
    74161 cggtcgtgtt ctgaaaaaca ctacgttaag tttatgatgc gcacgatgct gatcagttta
    74221 tctgtaggtg tctatatgca tgtacccgtt caggcaacga cacaaaccat caaggtcgta
    74281 cgtacctctg tttctgagac cactgattcc cctatcacta ttactcatac tttcactgat
    74341 gcaaatggaa aacagctact tcctgcattg acttcctata ataaagtcag ttatcctcca
    74401 acgataaacc actactggtt aattcctaac aaaacattat ggacatcgga aaccggaagc
    74461 agtgtgccgc tttctatctt ttccgatatg actgatccta caaaaggtga tcagttaaaa
    74521 cagatcactg atattttgaa tcatgctttg acagatataa aggacggtgg aagcgcacaa
    74581 gtttcttatg tatatgaaaa agattattca cagtttgaaa gaaagaaaat cgatgttctg
    74641 gcagggacaa aagtttcgat atctgatctg gtagaaaggc tgaagaacgc agatggaaca
    74701 caaggagata tgtccaaagt aatttcaaac aatgggaata tcgtggatac gactaataca
    74761 ggaacatcta ctatttcctt gtcctatttt gatccaattg ctttaaaaac aatgactgca
    74821 tctgctgtga tccatgttcg tgctgatcaa acagctatca aaggaaaaga tcttcactta
    74881 agaacaggag agagttacgc ccctaaagat cttgttgaat atgtaacaaa aagcgatgga
    74941 acttcaggaa ataaagaaga aatcaaaatg aacgggacag tagatactac aactccggga
    75001 gaatatcccg ttgtattaac ttatatagac tcgaaaacat caaatgtggc agaaacaaca
    75061 gctgtagttt atgtagaagc atctgctgct tcatcgactg tcatcactca ttttatagat
    75121 agtcaaacaa ataaagaact tcaagcagaa acacatatga gtagtgtaaa tcgtattcct
    75181 gatttgcgcc tacctgctga attttctgat cgagtgccaa atatagattt atcgacttat
    75241 gaacaaaatg ggaaagtcac ttctatttct gattggatgc aagcaaacgg attagacaga
    75301 aaaagtcact ggtcggagat ccttgaagta cttcactcag aactgatgaa taataggaag
    75361 caatcaggag gggaaattac ctttacttat gtttatatac cagacaatcg ttcattagaa
    75421 ggtcatgatc ttattgttca tccaaacgaa aaaataaaca aagaagagtt gatccaatca
    75481 gcaacaaatt ccttaggtaa acccgtaccg ttaaaggatg tatcggttac aatcaatgaa
    75541 gtaccattgg aggatggtta tttgaccaaa caaacaggtg attttacgat tatgtatagt
    75601 tatcatgacc cgtattcact ccaagaactc catgctcagt cactattaca tgttttacca
    75661 ttaaacaaac agatagatca aaaagaacaa aaaagacgag aagaaagctt gtcagaaaaa
    75721 aaacaacaaa tggctcatgc agtttctcca acagggaaag taaaacaaac aagaagccta
    75781 gagttagcaa gaatatctag aaaaatctca gaatctaata aaacgataag gcaagaagct
    75841 catttggccc cacaagtttc tggattggaa aatgaaattt tagagcctaa aataaaacaa
    75901 caatcagaaa aagaatttgt tgctaaacag tcggaagatc agaaggaatc agatgaccag
    75961 aaggaaacga agaaaaaacg cttgtcaact attgattcca ccgatgagca tctgcagccg
    76021 caagtaccac ttcctagtcc gggaacaccc gcacctggtg gaggtattcc ttcttctgct
    76081 ttaggagatt tcatgcgagg tatggcagga acatgggtat acgctaatcg aaatgaattt
    76141 gacgcagatg actggttctg atttatatct ctatctgtta tagtcaaata agtagctgag
    76201 tcatatgtta tttggcataa tggagacgag gagggataga aaaatgttag ataaaacgtt
    76261 accttacgca gaaatctgga tgacaagacc acttcaacaa aatgttccaa tcacgccttt
    76321 agcggaaggt tttgaatttc gattttatca aaaagaagat gaatctgctt gggcggagat
    76381 tgagacttcc gttggggaat ttcaaacgac agatgaagca cttgagtatt ttcaacaaaa
    76441 ctttgctcct tatcctgaag aacttgaaag aaggatgttt tttgtagaga caaaaaaagg
    76501 agaaaaagta gcaacctgta ccgcttggcg aaaaaaatca agagagcaag tgtatccggt
    76561 ttttcattgg ttagcagtga aaccagatta ccaaggaaaa gggctcgcaa aagcattgac
    76621 tgccagagtc ttgcaagaat ttcctaccct ccatacggac ggaccaatct atcttcacac
    76681 acagacatgg agccatcagg cgattgctct ttataaaaaa atgggtttta cttttattgc
    76741 tgaaaatcta gacggcacac ctaatccaga ttatgaaaaa gttatgcagg ttttaagtaa
    76801 attagataaa taacgaaatg gtgaaataag tcctcagtat tgggataaaa aacgaaatag
    76861 tcattcctca tatcttactt ttttgagtta atactatcgg ataactaatg gcatgccata
    76921 cattcaaaaa agaaggggca gtgacaaaag tattgtcact gccccttctc ttgtttttta
    76981 gcatgtttta tcgtttgatt tttttaggaa taaaattctt gatgaacgta aatttttggt
    77041 aatcgtttcg actatagagg taaccaaata cactgaaaac aactgctcca ataaaattaa
    77101 caaataaatc cttcatagta tccatgattc ccacgtctaa gtaaccattt tggatgattg
    77161 ttttatctgt tttaccatct gcattttgac tttcaatgat tgtccgagtg atatctggaa
    77221 catgatagac agtgtttccg tctttactta actccacact attgatttgc tggatgatcg
    77281 tatctttttg catatcgaat tgcagtaatt tatctgctga aaattccaca aactcccaca
    77341 taacaccaac tgtcatcgaa aaacagaagg aaacaactgc tagtaataaa ggagtcaagt
    77401 tcatggtttt tgtattttta ttgagcaaat aaacaaggga aaagccgaca cccccagcta
    77461 aaaatccgct gaatgcatgt agaaaagtat cccacattga aaaatagcca taaaaatcac
    77521 ttaactctcc taaaactgtt gaactaaaaa taaaagataa caaaatgatt tctagcatat
    77581 ttggaatctg tatctttaat atcttttcca gaaaataagg gatttcaaat aaaattaatg
    77641 tcagtaataa aagcattgta tttccccagt gttggttcca gacctgatcc actagcataa
    77701 taacgacgat gatacgtaat aaaatataga ttccgtttgt gattctacct gcagtttttg
    77761 tactgttcgt aaaaaaggtt ttcaaatgat tcataaaata actccttcat gtatatcctt
    77821 aattatacta agaagcgtgt gtttaccaaa gctatatgac ttaaagaaaa acagagttga
    77881 tattgtgaaa aaaatcactt aatatagtat aaggagaaaa gggggataca aatgaaaaaa
    77941 ttagttgctg gtttattaac agccgttgca cttcttgcta tgagcgcatg cggcgcagat
    78001 cagaaaaata cagcgcagac aagttctagt acgaaggaat ctgccgaagt atcatcaggc
    78061 gcttctgaac aagtatatac agatccaaaa gaaatgaaag aatcttatga tgtcgtgatt
    78121 attggagctg gtggtgcagg gatggcagca gctatcgaag caaaagatgc tgggttgaat
    78181 ccagtaattt tggaaaagat gcctgttgcc ggaggaaaca catctaaatc atcaagcggt
    78241 atgaatgctt cagaaacaaa atttcaaaaa gaagaaggaa tcaaagactc gaatgatgcc
    78301 ttctatgaag aaacgctaaa aggtggtgga ggaacgaatg ataaagaaat gcttcgatac
    78361 tttgtcgatc actctgctga tgcggtcgat tggctagatc aaaacggtat caaactggac
    78421 aacttaacga tcactggagg gatgagtgaa aagaggacac atcgtccgtc tgatggctcg
    78481 gctattggtg gatacttagt ggaggggtta ttgaataacg tccatgagcg ggaaatccct
    78541 atttttgtca acgcagatgt gacagacatt aagaaaaatg gggaaacggt caatgaagtg
    78601 actgtacatg ttcaaggaga agaaccaaaa gaggtcaccg gaaaagcaat cgtagttacc
    78661 acaggcggtt ttggtgccag caaagaattt attgaaaaat atcgtcccga tttagctgat
    78721 tatattacga caaatcaaga aggatcaact ggagatggta tcaccatgat tgaaaaatta
    78781 ggtggacaaa ccgttgatat ggataaaata caaattcatc caactgtgca gcaggatgag
    78841 ggggtcttga ttggtgaggt tgttcgcgga gaaggagcaa tcttggtcga tcaagagggg
    78901 aaaagatttg tcaatgaaat ggatacaagg gataaagtat ctgcagcaat tacagcattg
    78961 cctgaaaaat cagcttattt gatttttgac caaggcgtgc gtgatcgtgc cacagctatt
    79021 gaattttatg accaaaaagg atatgtcaca actggaaaga caatggaaga attagcagaa
    79081 aaaatcgatt taccaaaaga aacactagca aaaacagttg atggatggaa tacggatgtt
    79141 gcggagaaaa aagatgctca attcaaccgt gccacagcca tggaacatga cttatcgtct
    79201 ccgaattatt atgcgatcaa aatcgcacca ggaatccatt acacgatggg cggtgtgaaa
    79261 atcaatacga acactgaagt attagataaa gaaaaccagc caattagggg gttatatgct
    79321 gctggtgaag tcacaggtgg cttacatgga aacaaccgga tcggtggaaa ttcagttgcg
    79381 gagatcatta tttttgggcg acaagcgggc atacaagcag ctcgatttgc ttcggaaaat
    79441 gaatgaaata gaacgtaaat aaaaagaggt tgtgaaatca gcgttttgag ctaataccga
    79501 gggtcagctg attgaacact gtttagtgaa gaggttgtga aaaagctgcc ctgcatcaag
    79561 taataagaac agccaagtaa aaacagcttc ttatgttttt aacttggttg gacttattac
    79621 cgcagatgca agcttttgaa caccgtttat tcggaaaaag agcgtgggac aaaagtaaag
    79681 aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaaga aattccttcg
    79741 gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc
    79801 ttggagttaa acgcttctgt cccagcctct tttttgatca gcatgatttt attcagttat
    79861 cttcaaaatg atttttccga tgtttttgtt tgcttccata tattcatgcg cttgtttgac
    79921 tgcgctgaat tggaatgtac ggtcaatgat tggccgaatc ttgccatttt ctagaaaagg
    79981 cccagctttt tgcataaaag tttgtgcgag ctctgcttta tactgatcac ttctaggtgt
    80041 caataatgta ccaagcagtt ggacacgttt cccaatcaat acaccaaggt ctatctcagg
    80101 cacgatggtc ccacctagca taccaatcaa gacccaacgt ccatcaacgg ctatagaagc
    80161 taaattcttt tcccagtaag aagcgccgat gaaatccaaa ataacatcga ctccccgacc
    80221 ttcagtgatt tttcgtacct cttcttgaaa gtcttgtttt ttatagttga tcaattcatc
    80281 tgcaccaagt tctttacaaa aggcgagttt ttcttctgat cctgcagtaa cgatgatttt
    80341 cgcgtgagaa agctgtctag ctaattggat cgctgctgtt cccacaccac ttgctcctgc
    80401 atggatcaag acagtttcgc cttcttgtaa ttttccaagc cagtaaagtg tttgataagc
    80461 agtcaaaaaa acttctgaga ttccagcagc agaaacatag tctagcgtat cagggagaag
    80521 gattgcacga tcggcaggca tcacagcata ttcggcataa ccacctagat tgactaatcc
    80581 gtatactttt gtcccaggag taaaagtgga gatatcactt ttattctcaa ctacctctcc
    80641 agctacttcg actcccaaaa tagggtaggg aggtttcaaa gagactgatt ctcttttcat
    80701 aatatctgtt cgattgacag ctgctgtatg gactttgatc agtaattcgc cttgttttgg
    80761 tttaggaaca ggcacttcaa tttgttctaa tacatctaca gaaccaggat gcttgattga
    80821 aatggcacgc atggatctat tcctcctttg cccattcttg gaatgctgta ttcaactctt
    80881 tgctaccttc agaaagtact agagtcgtac gagaaatatc cgtttcttcc actagagcgg
    80941 caagtacagc agcaactgta gagcgagtga tcgtttttga tgccttctca ttaggcttag
    81001 ttaattgaat ggaagtaact tcttcatcgt tagtcaatgt gactggttga agaatgacaa
    81061 aatctaagtt agttcgtttc agccattcat ctgcataatg tttagtaata taataatctg
    81121 tcatggactc aggccatttt gctggttcat ctgcataaac agcactgacc atcagatagc
    81181 gagagacatg cgcattttca gctgcgatca ctgttttgat tgcgccatcc aagtctactt
    81241 gcagaagatt ctttccttga gagccggcag caaaaatcaa tacatcgatg cctttgaatg
    81301 cttcggtcat tttttccacg gaccaagtta aatcaaaaga gacatacgaa acattctctt
    81361 tttttactaa tgtttgggaa gcaacatctc tcacccctgc aaaaatttca tgggaacttg
    81421 gagctaaatc ttttatcagg tgtcggccaa tttgtccgtt tgcacctaca acaaaaattt
    81481 tcatttggat catcctttcg acgtcaaatc tttccattca atagtaacac caaaagagaa
    81541 aaaaatcaga gatcggctac gatgactatc agttagtaag agaagggtgc ttgaagtatc
    81601 ttaacaagac attgcatact gctgcagtcg caagacctgc gacgattcct tcaggtatac
    81661 cacttgtaaa aataaccgct agaatagctg aataaacagc atttaaattt gttccgatga
    81721 ctttggcata atcttgctgg aataaaaagt agatcatatt catcactaaa atcgtattgg
    81781 tcaatgaacc ggaaagtcca gctaagaata aagaaacgga atctgctttt tgtttcatta
    81841 gtttgcgcag acctttataa acgaaataag gaaccacccc aatcaagatg cgaggaataa
    81901 acgcgatcag caaggctttc cagcttccat gttctgttcc gatgactggg ataaacggag
    81961 aaaagacaaa agacaaaggt gtttggataa ctgtgctacg gatcatgctg atcagtccaa
    82021 atgtaccgcc aagaaaagca cctagtcttg gtcctaaaac gatcgaagca atgattacag
    82081 gaatatgcat ggttgttgca ttgattggac cgatagggat aaaacctaaa ggcgtaacag
    82141 ctaataaaat caaaatagct aaaaacatcg cagttaatgt aaagtttttt gtatttttca
    82201 taagtactcc ttttttctaa gcatttcttt cattaagagt cagcatgacc tgttcgatga
    82261 tcgtctgaat atcagctaag gcaccttttc caaaatctcc acaagctaaa agagattctc
    82321 ttggttcaat ttcctgatag ccgacagatc tcaatgtttc cagattacgc tgagtgatcg
    82381 ggttttgata catattcgtg ttcatggcag gggcaatcaa ttttggtgtt cccactggaa
    82441 gcgccattgc gactgttgaa agaagatcgt ctgcaatgcc gtttgctagc ttgccgataa
    82501 tgtttgctgt agcaggcgcg atgagaaaca agtcagcttg ttttgctagt tcgatatgat
    82561 tgattacctg cggatttgat tcttgcatga cgtctgtatg gactgcgcgc ttcgacagag
    82621 attgcaaggt taatggcgtg ataaaatgtg tgctgctttt agacataatc acatctacct
    82681 gggcacctaa tttgacgaat tgacttgtaa gatcagcgct tttgtaagca gaaatacttc
    82741 cagtaacgcc tagtaagatc tttttcattt tgattcctcc agtctaattt tctcagtaat
    82801 caatgcagca atctcagatt tactttgagc ttcttccact gttccatctt tagataagag
    82861 atagccgtgg tgtgtctcgc catggatttc cgtcaaatca tttgcgaaaa taaaatcagt
    82921 tttgttattt atcagactat tttttgcgac tgccagaaga gattcctttg gtacatctac
    82981 taatagtttg aaaccaatga tttttgcttt tggctgccat tcacgtagat aagaaatgat
    83041 ttttggtgtt ttcttcaaaa cgagcacaag atgctcggtg ttagaagaga tctttgaaac
    83101 agtggtatct gtctgttcat tttccgcaaa ccaattgtcc agttcttctt gcgttgaaga
    83161 agtgggtagg ttttttgcca gctgttcttc tgaaaaacta aaagcaggag taaaatcact
    83221 gactgccata ctgtggataa tcgcatcgta gtgttctttt ttcgttagtt cttctaattg
    83281 taaaaataag tccagtgcag attcgatcgt ataaatcgtt atattggagt gtcttttcgg
    83341 tttcagtgct tgtttcgtag tcacgtaatc gattgtggta tccggattgg cagctaaata
    83401 atcagctaat gcttgcccta atcgtcctgt cgaatgattt gtaatagcac gtacttgatc
    83461 gattttttca gtcgttcctc cagctgtgat tagaatcttc atttatggtc acctacattt
    83521 actagtgttc ttccttggtg tgttccagca agtagtttag atgctgtttc aggcaattga
    83581 tccagagtaa tctcttggac gaccagttca tctgcaatct gcaaatccgc catctgttgc
    83641 cataaagaaa gacgttgatc tattggtaca ttgacagaat caattccaat caactggatg
    83701 tttcgcagaa taaaaggcaa gacagtcgcg tttagtttga ttcccccagc attgccacat
    83761 aaagctactg caccgttata agagatcaat ggaagaagac tgcttagctg ctcgccacct
    83821 actgtgtcga tgacatagtc gatttgctgt tttcctaaag cttttgttgt ctctggcaga
    83881 aattcatcag gtcgtacgac ttgcaaagca cctagacttt ttagccatgt gacagactct
    83941 tcttttctgg aaaaggcaat gattgactgg aatcctagtt tatgtaacag ggctatcgct
    84001 gtactgccta caccgccaga agcaccagta acgacaactt ttttatcgga ggtcatacct
    84061 tgacggatta aagcgttcac acacaacata gcagtaaacc ctgctgtacc aaggatcatc
    84121 gcttgacgca gcgtcatatt ttttggtaga ggaactaacc agtctcctgg tactttttga
    84181 tattggctat aaccgcctgg atgggtgacg ccaagcccaa aaccagtagc aagtacaaga
    84241 tcttcttttg aaaaacgtgg attgttactt tggacgacag ttccggataa atcaatccct
    84301 ggagtcatcg gatatgttcg tatcacctga ccagactcgc tcattgccaa tgcatcttta
    84361 taattgacat cagaataggc aatttttacc aagacttcat tttcagccaa ctcagtaaat
    84421 ggcagatcac tgattgctgt atggaaaaca ggttccttta ttacctgaaa agatcgaaaa
    84481 gcgttcattc gtgagacact ccttttaaaa ttttctttta tcataccata aagcctactc
    84541 ttttattctt gttcttccgt aaatttagac aaaaattagg tgaaaaataa agaatcaaac
    84601 aactagaatt tattccaaaa gataaagaaa tattcagcaa aaaaaatttt acaaagggtt
    84661 gcctttttca ataaatttcg tatactaata cgagtggtag taaacaggaa gtttactatt
    84721 agaaaacttc gattcggagg aaattatgga aattggtgag aagttaagaa atttgcgcat
    84781 ccaaaagaat ttgacacaag aagaactagg cgaacgcaca gatttgtcaa aagggtatat
    84841 ttcccagttg gaacgagatt taagttcacc atcaatggaa acattctttt ctattcttga
    84901 agtcttaggt gtgactccag aagaattttt tcatcaagaa gcagcgaatc tgcaagtggt
    84961 ttattcaaaa gaagatcata cggtttattt cgatgaagaa aacgggtatg aattggaatg
    85021 gctcgtggct gattcgaatg aaaaagaaat ggagcctgtc ttgctgactt ttgaaggtgc
    85081 aggagaatat aagacgtttg aaccttcatt atctgaaacg tttatttttg tattagaagg
    85141 ggaattagag ttaagcttag gagagacggt ctatgtggca aaagcaggac agtcgatcta
    85201 ttatcaagcg acaaaacaac atcaattaag gaatcatctc aaaaaacgaa caaaagtatt
    85261 gattgttgca acgcaatcat atttgtaata tagggggtaa aaacgtgggg aaaccaatca
    85321 tttcatttga tcaagtagtg aaacgctatg atgatgaaac agtattaaaa aaagtaagtt
    85381 ttgagatcga acaaggaaaa ttttatacac tgcttggtcc ttccggctgt ggtaaaacaa
    85441 cgatcctacg tattatagct ggattcaccg aagctacaga aggggacatc tattttgaag
    85501 ggaaacggtt gaatgatttg ccagctaata agcgacaagt aaatacagtc ttccaagatt
    85561 atgcactttt tcctcatatg aatgtttttg ataacgttgc ttttggattg aagatcaaga
    85621 aaatgtccaa atcagatatc gaaaagaagg taaaagatgc tttgcgtatg gtccagcttc
    85681 ctggatacga aaaacgggag attagtgaaa tgtctggtgg tcaaagacag cgtgtagcga
    85741 ttgcacgagc aattgtaaat gaaccgaaag tattgctatt agatgaaccg ttatcggcgc
    85801 ttgatttgaa actacgtaca gatatgcagt atgaactgcg ggatctgcaa caacgattag
    85861 gaatcacgtt tatttttgtt actcatgacc aagaagaagc gttggcaatg agtgatgaga
    85921 tttttgtcat gaataaaggg aaaatcgtcc aaagtggtac acccgtagat atttatgatg
    85981 agccaatcaa tcatttcgtt gctgattttg ttggggagag caatatcgtg gacggtgtaa
    86041 tgatcgaaga taatctagtt gagtttgttg gtaagcaatt tgaatgtgtc gatggcggga
    86101 tgcgtctaaa tgaaccagtt gaagttgttt tacgcccaga ggatttgacg atcacgacac
    86161 cagataaagg aaaactagtc gtaacggtcg atactcagct gtttcgtggt gtccattatg
    86221 aaatcatctg ttatgatgaa cagcaaaatg aatggatggt ccattcaacg aaaaaagcta
    86281 aagaaggaag caaagtagga ttggcctttg agccagaaga tatccatgtc atgcgtttca
    86341 acgagtctga agaagaattc gatgcacgtc ttgacagcta cgaagaatag gggggacaag
    86401 aatgaaaaca atgcgcagga tctattccgt cccttatatt ttgtggctcg gtttattcgt
    86461 gatcgcgcct gtattaatga tcatttatca atcttttttt gatatgaatg ggcaattcac
    86521 gctgaataat tatctatctt attttacgtc ggggacatcg ctttctatga cgatcaattc
    86581 tgtatggtat gcatttttga tcacgttttt cacgctgatc atcagttacc cgactgctta
    86641 ttttttaaca aaactgaagc acaagcaatt atggctgatg ctgattattt tgcctacatg
    86701 ggtgaatctg ctgcttaaag cttacgcatt tattgggatt ttcagtgtgc acggcagcgt
    86761 gaatcatttc tttgatttta tcgggatcgg gccgaaacag atattattca cagacttcag
    86821 ttttctgttc gtggcaacgt atatcgagat tccatttatg attatgccaa ttttcaatgc
    86881 attggaagag atgaatcctt ctttgatcag tgctagtaga gatttaggcg cgaatagctt
    86941 tgagaccttc cgacgagtga tttttccgtt atcattaaat ggtgtgaaaa gtggtgtcca
    87001 agcagtattt attccttctc tttctttatt catgctgact cgtctgattg gcggaaatcg
    87061 ggtgatcact ttaggaacgg ctatcgaaca gcatttcttg gttacacaaa actgggggat
    87121 gggttctaca atcggtgtga ttttaatcat tgctatgttc atcgtgatgc tcctgacagg
    87181 cgagaagaaa aaaggaggtc gctcataaat gaaaaaattt aaatggtcgt atctttattt
    87241 agggatcgtc tttttattct tatatgcacc gatcttctat ctgatttttt attccttcaa
    87301 tgaaggagga acgatgaaca gctttacagg atttacttgg gaaaattatg aagctgtttt
    87361 tgaagataca cgtttgatca ttatcgtatt gaatacgttc atgctggctt tcttatctgc
    87421 attgatcgcc acgatcatcg ggacatttgg ggctatggga atctattaca cgaaaaaacg
    87481 acgtgcacgg acagcgttgt tgagcatgaa taacatctta ttagtctctc ctgatgttat
    87541 tatcggtgct agtttcttga ttttctttac actactagga ttcggattag gttttcagag
    87601 tgtattgctt tcccatattg ctttcagcat tccaatcgtc gtcttgatgg tcttgccaaa
    87661 attgcaggaa atgaacgatt caatggtcga tgcggctaga gaccttggtg ctaataattt
    87721 ccaagtagtc aaaaatatca ttttgcctta tctttctcca ggaatcatag ctggatattt
    87781 tatggcattt acctattctc ttgatgattt tgcggttaca tttttcgtca caggaaatgg
    87841 ctttacgacg ctctcagtag agatctattc tcgtgcaaga caaggaatca gtttagaaat
    87901 caatgcgcta agtgcgttag ttttcttgtt ctccatgata ttggtcattg gatactactt
    87961 tattagtaaa gaaaaccgtc caaaacggct gaagaaaaac agaagagtac gcgagggggt
    88021 ggcacggctg cgatgaaaaa attacaatct ctgtttttag gtattctcat cattattgcg
    88081 gtgctgctgc ttggtgttcg tcaacttgaa aaaacaagtg gcatgagtgg agcaaaggta
    88141 cttaccatct ataactgggg agattatatc gatcctagtt tgatcacgaa atttgaaaaa
    88201 gaatatggat acaaagtgaa ttatgaaaca tttgattcta atgaagcgat gttcacaaaa
    88261 atccagcaag gtgggacaaa ctacgacatc gctataccaa gtgaatatat gatccaaaaa
    88321 atgatcaaag aaaaactagt acttccatta gatcattcga agatcaaagg attaaataat
    88381 attgatccac gatttttaga cttggatttt gatccagata atcagtattc gattccatat
    88441 ttttggggaa ctttagggat cgtatataac gataaattct ttgatgcaaa tcaaattaag
    88501 cattgggatg atttgtggaa gccggaatta aaagacagtt tgatgctgat tgacggtgca
    88561 cgagaagtta tggggctttc tttgaatagt ttaggctatt ctttgaatag taagaatatg
    88621 gcacagttga cagaagcagc aaataaattg aatcatttaa caccaaatgt caaagcaatc
    88681 gtggcagatg agatcaaaat gtatatgatc aatgaagaag cctccgtggc agtgacattt
    88741 tctggcgaag ctgccgatat gatgtgggaa aatgaacatt tacattatgt gatcccacca
    88801 gaaggatcca atctttggtt tgataatatc gttatgccaa aaaccgtgaa aaataaagaa
    88861 ggtgcctatg atttcatcaa ctttatgcta gaaccggaaa atgcggcaca aaatgcagag
    88921 tatattggtt attctacacc aaatgaagaa gcaaaagcgt tattgccaaa agacatttcg
    88981 tcggacgaac aattctatcc atctgatgac acgatctccc atcttgaagt ctacaaagat
    89041 ctaggatcga aatacctagg catctataat gacctattct tagaattcaa gatgtatagg
    89101 agataatggc aaaactcagt aagaatgggg acttatcaac gttctaaaga gctaaaaatc
    89161 gtcctttttc tatctgagtt ttccaatctt ctaccaaaag cggatgaaac gccgcttttt
    89221 ttgtgttatt ttattttttc taccacgttt ttctaccaga gaaaagtgga caaaaccatt
    89281 gtaaaatagg gcccgagacg ggctagtaaa tctaagattg aaaaagttac agaaggataa
    89341 agtaattgga aactatgcaa atatacttaa cccttctgta atctattaat aaaactaata
    89401 tatgaatcca gttcaatatt tgtagctatg aaaaaacata aaaatcatat tagataaaaa
    89461 ctaaattggt agataaatta ttttgtcaga tatctgaggc aaatattcga ctatttgtgt
    89521 ttcaaagtaa aaattggaat acaaatatag aatagacata ttgcagccga tagtttatta
    89581 tgcttttaga cacattaaac tggaggaaaa aatgaaaaag agcgctaaag atcgagtttt
    89641 tgatgagctg atacgactaa cagggaatat gtcttctgtc actgccgaga aaatttcgaa
    89701 gaatctaggt atctctcgtc agaatgccag ccattatttg acgagattag tggaagataa
    89761 aaaggtcgag aaattaccgg gaaagcctgt tttgtggaag cctttagatg aatatgcggt
    89821 tgtagataat acggagcaaa taaacgaagc ctttcattct gtagttggtc atgacggaag
    89881 tctgagagag gtcattcaaa aatgtatagc cgctgtcaaa tatccgccta acggactcag
    89941 tgtattaata aatggagcca cgggtgttgg aaaaagtttt ttagccacaa aaatttttga
    90001 atacgctatc catgagcaaa ttattgaaaa agatgcacca tttgctatct taaactgtgc
    90061 cgattatgcg gacaatcccg aactgctttc ggcaacactg tttggataca agaagggggc
    90121 ctttacgggg gctgaaaagg ataccgatgg tttattggca acggccaaca acggctatct
    90181 ttttctcgat gaggtccatc gcctatcgaa agaaaatcaa gaaaagctct ttctatttat
    90241 tgatactgga aactatcgtc cagtcggtga aaatgtaaat tggcattctg ctaaggttcg
    90301 ttttattttc gccacgacgg aaaaaggtga aaattattta ttagacacct ttgatcggcg
    90361 tattcaaatt agtgttatgt tgccaacttt tgaagatcgc cccattcgag aacgattaga
    90421 gctgattcaa ttattttttc aaaatgaagc ggatgtttta caaaaagata ttattgtttc
    90481 aagagaagcc ttgtctttaa tgttatctca tccctttagt gggaatattg gtaagctgaa
    90541 gaatatcatt aaaatcagtt gtgcggatgt gtatagtcga acacaagaag aaccattaac
    90601 gattgggaaa aatgagatct tgattcagtt aaatatgctg gaatccgaag tagaaagctt
    90661 gccgattgct agtttattga taaagaaaaa tcaaaagata aaaacggatc gaatcaaacc
    90721 gtttgattat gattcgattt tggaaaaaat tactcagctg ataaatgaag gtaatttcaa
    90781 tgaaaatttt tcttcgataa agaatgaagt acagcatttg gtgcatcaca ttgataagga
    90841 ttctttttta tcaatgagta gaaaattgat gattcagatg tttagaaagg tctgggtatc
    90901 cgttttagga aaaacgtacg gattgacgac gagtggacct gttgcagaaa ttattagcag
    90961 atcctatgtc atttccccaa gctataagac agacttaacc gggataaatc gtacattagc
    91021 tgcaaatcta cctcggacga cttacttatg taatcagttc ttggagcatt taccaccgct
    91081 ctcagaaaag gacgagaact ttctaagact gcttattgtc gtgactttat ctgattatgt
    91141 agatgaatcc atcgagctga agggcctgtt gcttgctcac ggagatagta cggctagcag
    91201 cattcaagca gtagtgaatc aattatgcgg caattatgtt tttgaagcgt tggatatgcc
    91261 agtagagagt aatgtgtcag agattgtcga atcgacaagg aatttcgtga aacggcaagt
    91321 gaatacagat agtctagtgc tgatcgtaga tatgggctct ttaaatcaaa tttacacaca
    91381 gatcaagaac gatttaaaag gggagttact tcttctgaat aatttgacga cttccattgc
    91441 acttgatatt ggcttgaaga tgacgatgaa tgcgccattt aaacagattg cggaggatgc
    91501 agcagacaag tattcaatca atgttcaata ctttgaaggc ttttctcaaa gtagcaacat
    91561 tatcatttcc tgtatgtcgg gtttggggat ttcggacaaa ttgaaagatg tttttcgtca
    91621 tcatttatcg gatgaatcca tcgaagtttt cacgaaggac tacagagaac tacgtgaatt
    91681 aatcgaccaa aatgatgaaa cttattttga caagacaaaa ttggtcatca cgacttcgga
    91741 tcttccacgt tccttttcaa ttcctaatat taatgtttat gacatcttag acggtgatgg
    91801 agcagccaat ttggagcata ttttgagtcc tgaaatgaag ccggcagaat ttaaccgctt
    91861 gattcaggag ctagtaaaat tcttttcgtt ggaaggaatt gcggaccgtt tgaactttct
    91921 gaacccaacg atcgtgatga gtgaagtgga aacagtcatt tctaaatatg aaaattacta
    91981 tcatttcact ttaaatggcc gtgtaaaact aaatctctat atgcatacgg cattgatgat
    92041 ggaacgttta ttcttggctc ggacagaaaa atctgaagaa ccgaattatg atttgaaacc
    92101 agatgaagat gaattttatt atgtttctag aagtattttt caatcaatgg agatgaaata
    92161 taattttaaa gtcaatgctt atgagctttc gctgctctat gaattattag gaccgtatat
    92221 tcaaaaataa agtgtctaaa aaagtgtcta atctgttttt tttaggttag acactttttt
    92281 taatgtgaat taacgtgatt aaaccaatgt ttttaaaagt tggcacagga attgcttata
    92341 taaaagtgaa cagaaagaaa ggaattaacc atatgaagaa aattctaata gcgactcacg
    92401 gtcacttagc ttcaggcttt cttagcagta ttcaattact gacggggaag gttcaagaga
    92461 ttacggtgat taatgcttat gtggatgagt gtgattttga acaggagttg aatgtatttg
    92521 taaaccaggt gaacgagcag gatcagattt ttgtttttac cgatttattt ggcggaagtg
    92581 tcaatcagaa gattaccaaa acctttttgg agaaagagat tgctgtaact attttgtctg
    92641 gctttaatct gccaattctg ctcgaaatag tattggcaac caaggaatta acaaaggagg
    92701 atattcagga actggtaaat aaatgccaac aggaattaaa gatgaccatt cttgatgagg
    92761 tacatgtagt aaatgaagag gaagattttt tttaggagga agaaaaatga ttaaagcagt
    92821 gcgaattgat gaacgattaa ttcatggaca agtagcgatg acttggacaa agagtctaaa
    92881 tttaaccggg ttagtagtag caagcgatga ggcggctacc aacgatattc aaaagatgac
    92941 attgaagatg gctgcaccat cgaatgtcaa agtcattatt aaaacagttg atgacgcgat
    93001 tcatttgtta aaagatccgc gggcgaagga catgcgcttg atggttttag tccctaatgt
    93061 caaagatgcg gtaaaagttg cgaaagcttt cccaacggaa atcgaattaa tgaacgtggg
    93121 gaatgctggg aaaatgacac cgccatcgga agataagaaa accttatcaa aggaagtgat
    93181 gttaacgacg tcagaaattc aagacttaaa agaattagtt gaactttatc cagataccgt
    93241 ttttcagcct acaccggcga tggaaaaacg ttcagctatc tctattttga gtgattttta
    93301 gaaaaaggaa ggagaaaaat tatgtatgat gcatttgtag tcgctttggc cgtttttatt
    93361 ggaattgctg gacatgaaat gttcgggatg tccatgctga gtcgtccgat tgttgttgct
    93421 ccgttagcag gagcgttgat gggggatttg cagacggggt tgattgtcgg ggcgtcacta
    93481 gaagccatct ttatgggtgt cgtcaatatc ggaatctcta gtacggcgga gccttctttg
    93541 gctgctgggc tggctacagc gttcgcaatc aaaatgggag gaaacgtgga tgcgattatt
    93601 cccattgtct ttccactagc agtattagga ttacagttaa tgaatatgat tttttctttt
    93661 gtatgtggac cgatggctgc taaatttttc aaatttgcaa aatctggtga tcaaaaagga
    93721 atcatcaatc tgcacttctt tatgtggttt gtccactatg cgctttatgc tcttattcca
    93781 ttctttgccg ttttatttgg agcagatgtt gtcaaaagcg tcttggataa tattccagag
    93841 gtcatcatga acggattaac agtcgctgga aatctgctgc cggcagttgg gatggcgatg
    93901 ctgttacgga tgctttggag taaaaatatt gctgtgtggt ttttctttgg ggccgttgtt
    93961 atggcgtatc tcgagttacc attgattgca attgccgcag ttggaacgat tattgcaatt
    94021 gttatagccc aaagagatct acaactaaaa aatatatctg ttcatgcagt tcaaagcgat
    94081 gctgctgtaa atgcaacgct tgaccaagaa gaggaggatt tctttcaatg aaattagctg
    94141 atggaatttc taaagatgag aaaaaattgg ttcggaagat gttttggcgc tcgatgacta
    94201 tgtatgcttc ggtgaatccg gtgacgatgg gcggcggtgg tttcgcctat tctatgatgc
    94261 ccttcattaa tcatttctac aaaagtgaag aagatcgagc tgcggcgctg gaacgtcata
    94321 cacagtattt ttcgacgacc attccttgcg cgtcctttgt catggggatt gcggcatcga
    94381 tggagaagga aaattctgaa aaagctggtt ttgacgtgga aagtatcaac gcgatcaaaa
    94441 cgagtttgat gggaccattg gctggaattg gcgattcatt gttctgggga gtttggcgtg
    94501 tcgttaccgc gggactagcg atcaacttag ccaatgccgg aaatattttg gcaccaatta
    94561 tctttttagt tatgttcaat atacctaatt acttgatgcg atactatggc ggcgttttag
    94621 gctattcatt aggctctaaa tatattgaaa agctgtatgc gaacgggtta attgaaattc
    94681 tgacaaaagc tgctagtatc ttgggtctga tcatggtagg tgcaatgacg atcagtatgg
    94741 tcaaattcaa taccgttttg agcttttcaa tgaagggcga agaactaatg aatgtccaag
    94801 aaatcctcga tagtattttt aaaggaattg taccgttatt ggtaacattg agctgtttca
    94861 aattactaga taaaaaagtc ggcgtcatca aaattatttt tggaatggtc gtcttgggaa
    94921 ttttattgtc attacttgga atcgcatagt aggaagctgc agctactagg ctgcagtttt
    94981 tttagcaagg agggaaaaga tgactattaa tgaagtaatt tattcaccca aacacccatt
    95041 ttttaatgct gagttaaacg acggaaatgt gactacttca aacgaataca gctactatcg
    95101 gaatattcct gatgtaaagg gattgacctt cgatgtaagt aatcaaaaga cattagcaaa
    95161 ccttgatgtt tgcgggacga ttcaaacgtt gaccttttat cgagaaaatt tactaactga
    95221 ggaaaaacca ggtgtttggg tcaataaaca gctctctcaa accaattcgt tatccttaaa
    95281 ggttgaagta aacgggattg tctaccattt gtcagaatcg gatcatcgta ttgaagtcga
    95341 tgtgattcaa gactgtttgc ctagatatat tcatcattat tcgacgttta aggtcattgt
    95401 agtatccttt gctcccatac tggctaataa gcgtttaagt atgcttgttc aacaggttta
    95461 cattgtgaat gaaacacacg aaagccttca agtttcagtg aaagatgtgc cttcgtatca
    95521 agagaagtat tcggatcagc aaaatgtgtt gatttctcaa aaaggaaata aggacacgat
    95581 cgcacaacag gaagtggcaa gcttcgccat tgcttttgtg gacccaaatg cttttgaaga
    95641 agtaatgact tttcaggaat ctgatatcga aaaatggctg aaggaaacat tgaactattt
    95701 tgctcagcta tttgggcagc tgtcaatgcc aaacgataga gttgtccatc tctatcgtcg
    95761 tgcactttat caaagctttt ctagtttcgg tatgaatcga cagggacagg tcgtcggcag
    95821 caattggggc agctatcctg ctactaatcg aatttggaat aaggatatgt attacgcatc
    95881 attgccattc accatgtttg aaccggagtt atgtcaaaaa acggttctat ggtttgatca
    95941 atatggcgtt aagtttcctg gttcaaaatt tacaggaggt atcaatcatt ctctgagtaa
    96001 ttcgttatcc agtgcgttac tggctagtct ttattttgaa catactgggg atacaaaatt
    96061 ctttgagcgg tgtccgcagg ttctactgaa tgcttcaaga attattgagg acatattagc
    96121 tcaacgtcgg tcaggggaac caatgctttt tccatctgtt tggttatcag acgcctttgc
    96181 acttggaaag tatcataccg gcagcaatct ttgtctttgg aaggcctgta ttggacttgc
    96241 agaaattttt gaagccctag aacaactgag tcttagcaag cgatacaaaa acattgcctg
    96301 taaactaaag gaatcaattt gtaaacaaat gactatcgat ggtccttttg gtgagcaatt
    96361 tttggaggga atcggtgatg aggagaaaac aacctattca gtgaagcact atcaaaaacc
    96421 gattcttgag caagggttaa tctttttaag tgatgtgatt gtggatggaa aaattaattt
    96481 attgatgcat gatggagaag aatcagacac tacgttgatg cctttttatc aatttttaga
    96541 taataaagat catttatatc agaacaccat ggccttttct gctagttctt tcaatccaac
    96601 ctatagcgag gaaatcaaag gcatcacttg gggattagaa tccggcgcca cttttcctgg
    96661 ctttatcact gttttaatga gcgagctgca caaccctcaa gctttcgtaa cccgattaga
    96721 agaactaatt tgtttggcag atttagacgg ttcgtggtgg tggtggccgt atcgcttaga
    96781 ggctcaacaa ggagaagtcg tgcgagactt tggctgcggc aaatgcggct gggcatcggg
    96841 tctttttgtc agcctaatga tcagtcaata tttcggattg aaattcaaaa aaggagtatt
    96901 gcaaattgat cctgttgata atttaacgtt ttcttggaag aatttgaagt tcggtcaagc
    96961 cttcatttcg attgaatgta cgcaatccga gttgagtatt tcaaatttgt cagaaaagcc
    97021 attgaaaata agtgatacga aaaaaattct tcacgtagaa aagggaaaaa cgattcacat
    97081 tcaaaggaga aaaagatgag tatactaaaa aatattaccc aatttaaaaa aatccagagt
    97141 cgagcaatca ctcccgaaaa tccaacaggc gagaaaggta aagctgcgat gttgccaagc
    97201 gagctaggac caagccgcaa aggtcgtggc gcaattccgc tgacttctgg tgaagaaact
    97261 gtgattgcgg atattactgg aacgggaata attcgtcata tgtggatgac gattcgtgat
    97321 cgaacagaat ttggcagttt tgttttacgt gatttaatct taagaattta ttgggatggg
    97381 tcggatagcc ctgctgttga aagtccttta ggggatttct tttgtaatgg gtttggagag
    97441 cgttgcgagg tgaactcgtt gccgatagtg gttaatccta cagggggctt taattcctat
    97501 ttcgaaatgc ctttccaaac atccgctaaa attacgatcg agaatttaca tccgaaggat
    97561 atgtcctcat ttttttatac gataaattac gctttggtgg acgaattgga agaaaatagc
    97621 ctatattttc acgcaaattg gcatcgggaa cgcattacca aattacagga ggattataag
    97681 ttaattgatc gattaaaggg tcaaggctat tacgtgggta cttaccttgc gcttacctgc
    97741 ctagaacgtt attggtgggg cgaaggcgag tttaaattct acattgatgg ggatagtgag
    97801 tatccgaccg taacctcaac aggttcagaa gattattttg gcggggcgtg ggcctttcat
    97861 gagtacgatg agttcggacg tccacatgcg aaaaactatt caacagcctt tttagggtat
    97921 ccttaccaat caaatagaga tgccacaaga gatcgttttg aaacgggaaa attgaatgcc
    97981 gttcatgcct ttggtgacga cgcaatgcca cgacatggtc tttatcgttg gcatcttcaa
    98041 gaccccatct gttttaccga agatttatcg gtcactttac aacaaatcgg caacgatgat
    98101 gttcgcttat ttgagcggca agacgatgtt gctagtgtcg cttactggta ttcaacagaa
    98161 gcttcaggaa atactaaacg tcatttcact gatcgagaac gcttgccgcg ttaatgaagt
    98221 gtttttcaca tattcgaagg gaggaaccta tgatgatata tggggcaatt gaagctggag
    98281 ggactaaatt tgtctgtgca gtcggtactg aggaattaac gattttggaa agagtcagtt
    98341 ttcccacaac aactccagtt gaaacaatga agcaggtcat tgattttttt aaacctttta
    98401 aggaacaatt ggcaggcatc ggggtcggct gctttggtcc aattgatatt caatgcaaat
    98461 caacgagttt cggtcatatc acatcaacac caaaacttgc ttggcaaaat ttcaattttg
    98521 taggaacgct aaagcaatat ttcaatattc cgattgaatg gacaacagat gtcaatgcgg
    98581 cttgctatgg tgaatatgtt gctggttctg gcaaagggtt agcaggtgtt gcatacttca
    98641 ctgtgggaac aggtatcgga ggtggtgcct tgttcaatgg gactttcgtg gaagggttca
    98701 gtcatccgga aatggggcat atgttgatca agcgacatcc aaaggacaat ttttcaggaa
    98761 actgtccctt ccaccaagat tgtttagagg ggatggcagc aggaccagcg attgaaaaac
    98821 ggttgggtgt aaaaggccag aatttattgg ctgacgattc gttttggcag attgaagcct
    98881 tttaccttgc ccaatgtgca tacaatacaa cactgatgtt ttcgccagat agaattattt
    98941 ttggcggtgg tgtaatgaaa caagaacaca tgaagaaaaa agttcaggat aaatttgttg
    99001 agcttataaa tggatatgtt gaaataccac caattgatag ctacattatt acgcccgaat
    99061 taggtgataa tgccggaata atcggcggat tagcattggc cagaaaagcg gtaagaaata
    99121 agcaaccata attaatgaat gtttcattga tactcatgtc aaaagtattt aaccgagtaa
    99181 tcaaatgatc aaatgaagtt ggataaagcg aaaatgaatt caaataaaaa ggctctaagt
    99241 caaatcgtgt tgttttcatt aaatcactga aaggaaatgc ttctgcgttt ccttttttct
    99301 ttttgttgtg cgctgtatac cttgaggata ttcactggtg gcgtacactt aaatagtaat
    99361 atattgtgtt actttatgtc tgtggtatta gttatagttg cttaccaatg tatgttcatt
    99421 tttcgaaatg ttgacttcat aatccttggc ttaatgtttg aatttttttg gtgatagatt
    99481 ttttcagccg tataaaataa atcgtaattc tattatagag agttgtgatt ataaaaagat
    99541 agcaagaaaa aaaaagaaag tttctataca ttttaaatat tttattccta tttctattgc
    99601 taaaaaagta gaaattctat atttaccttt aactctcgct tctcttgcaa atgtagttag
    99661 ca
//
LOCUS       DAJSAW010000003        82985 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJSAW010000003 DAJSAW010000000
VERSION     DAJSAW010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.003"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            22..540
                     /locus_tag="ONT64_000229"
     CDS             22..540
                     /locus_tag="ONT64_000229"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR2301673.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            668..1351
                     /locus_tag="ONT64_000230"
     CDS             668..1351
                     /locus_tag="ONT64_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCR2301674.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1660..1733
                     /locus_tag="ONT64_000231"
     tRNA            1660..1733
                     /locus_tag="ONT64_000231"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1694..1696,aa:Arg,seq:tct)
     gene            1892..2251
                     /locus_tag="ONT64_000232"
     CDS             1892..2251
                     /locus_tag="ONT64_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HCR2301675.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2374..4005
                     /locus_tag="ONT64_000233"
     CDS             2374..4005
                     /locus_tag="ONT64_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HCR2301676.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4067..4708)
                     /locus_tag="ONT64_000234"
     CDS             complement(4067..4708)
                     /locus_tag="ONT64_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR2301677.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4894..4970
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4990..5298
                     /gene="rplU"
                     /locus_tag="ONT64_000235"
     CDS             4990..5298
                     /gene="rplU"
                     /locus_tag="ONT64_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR2301678.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5325..5669
                     /locus_tag="ONT64_000236"
     CDS             5325..5669
                     /locus_tag="ONT64_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCR2301679.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5682..5975
                     /gene="rpmA"
                     /locus_tag="ONT64_000237"
     CDS             5682..5975
                     /gene="rpmA"
                     /locus_tag="ONT64_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR2301680.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6409..9216
                     /locus_tag="ONT64_000238"
     CDS             6409..9216
                     /locus_tag="ONT64_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2301681.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9407..9898
                     /locus_tag="ONT64_000239"
     CDS             9407..9898
                     /locus_tag="ONT64_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2301682.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10001..10990
                     /gene="manX"
                     /locus_tag="ONT64_000240"
     CDS             10001..10990
                     /gene="manX"
                     /locus_tag="ONT64_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR2301683.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11023..11826
                     /locus_tag="ONT64_000241"
     CDS             11023..11826
                     /locus_tag="ONT64_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR2301684.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11847..12761
                     /locus_tag="ONT64_000242"
     CDS             11847..12761
                     /locus_tag="ONT64_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR2301685.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12844..13218
                     /locus_tag="ONT64_000243"
     CDS             12844..13218
                     /locus_tag="ONT64_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HCR2301686.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13357..13602
                     /locus_tag="ONT64_000244"
     CDS             13357..13602
                     /locus_tag="ONT64_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR2301687.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13774..14838
                     /locus_tag="ONT64_000245"
     CDS             13774..14838
                     /locus_tag="ONT64_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCR2301688.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15084..15509
                     /locus_tag="ONT64_000246"
     CDS             15084..15509
                     /locus_tag="ONT64_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR2301689.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15502..15951
                     /gene="nusB"
                     /locus_tag="ONT64_000247"
     CDS             15502..15951
                     /gene="nusB"
                     /locus_tag="ONT64_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR2301690.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16048..16902
                     /locus_tag="ONT64_000248"
     CDS             16048..16902
                     /locus_tag="ONT64_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HCR2301691.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16903..18240
                     /locus_tag="ONT64_000249"
     CDS             16903..18240
                     /locus_tag="ONT64_000249"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR2301692.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18245..18469
                     /locus_tag="ONT64_000250"
     CDS             18245..18469
                     /locus_tag="ONT64_000250"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR2301693.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18471..19361
                     /locus_tag="ONT64_000251"
     CDS             18471..19361
                     /locus_tag="ONT64_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCR2301694.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19370..20188
                     /locus_tag="ONT64_000252"
     CDS             19370..20188
                     /locus_tag="ONT64_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HCR2301695.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20295..20744
                     /locus_tag="ONT64_000253"
     CDS             20295..20744
                     /locus_tag="ONT64_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCR2301696.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20762..22444
                     /gene="recN"
                     /locus_tag="ONT64_000254"
     CDS             20762..22444
                     /gene="recN"
                     /locus_tag="ONT64_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR2301697.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22499..22615)
                     /locus_tag="ONT64_000255"
     CDS             complement(22499..22615)
                     /locus_tag="ONT64_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HCR2301698.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22688..23644)
                     /locus_tag="ONT64_000256"
     CDS             complement(22688..23644)
                     /locus_tag="ONT64_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR2301699.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23815..24183
                     /locus_tag="ONT64_000257"
     CDS             23815..24183
                     /locus_tag="ONT64_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HCR2301700.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24353..24784
                     /gene="mraZ"
                     /locus_tag="ONT64_000258"
     CDS             24353..24784
                     /gene="mraZ"
                     /locus_tag="ONT64_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR2301701.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24805..25764
                     /gene="rsmH"
                     /locus_tag="ONT64_000259"
     CDS             24805..25764
                     /gene="rsmH"
                     /locus_tag="ONT64_000259"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR2301702.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25769..26170
                     /gene="ftsL"
                     /locus_tag="ONT64_000260"
     CDS             25769..26170
                     /gene="ftsL"
                     /locus_tag="ONT64_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR2301703.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26171..28363
                     /locus_tag="ONT64_000261"
     CDS             26171..28363
                     /locus_tag="ONT64_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCR2301704.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28389..29351
                     /locus_tag="ONT64_000262"
     CDS             28389..29351
                     /locus_tag="ONT64_000262"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR2301705.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29365..30726
                     /locus_tag="ONT64_000263"
     CDS             29365..30726
                     /locus_tag="ONT64_000263"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR2301706.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30752..31840
                     /gene="murG"
                     /locus_tag="ONT64_000264"
     CDS             30752..31840
                     /gene="murG"
                     /locus_tag="ONT64_000264"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR2301707.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31855..33075
                     /locus_tag="ONT64_000265"
     CDS             31855..33075
                     /locus_tag="ONT64_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCR2301708.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33231..34556
                     /gene="ftsA"
                     /locus_tag="ONT64_000266"
     CDS             33231..34556
                     /gene="ftsA"
                     /locus_tag="ONT64_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR2301709.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34579..35820
                     /gene="ftsZ"
                     /locus_tag="ONT64_000267"
     CDS             34579..35820
                     /gene="ftsZ"
                     /locus_tag="ONT64_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR2301710.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35837..36514
                     /locus_tag="ONT64_000268"
     CDS             35837..36514
                     /locus_tag="ONT64_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCR2301711.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36528..37127
                     /locus_tag="ONT64_000269"
     CDS             36528..37127
                     /locus_tag="ONT64_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCR2301712.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37142..37429
                     /locus_tag="ONT64_000270"
     CDS             37142..37429
                     /locus_tag="ONT64_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCR2301713.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37478..38260
                     /locus_tag="ONT64_000271"
     CDS             37478..38260
                     /locus_tag="ONT64_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCR2301714.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38281..38988
                     /locus_tag="ONT64_000272"
     CDS             38281..38988
                     /locus_tag="ONT64_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCR2301715.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39250..42048
                     /gene="ileS"
                     /locus_tag="ONT64_000273"
     CDS             39250..42048
                     /gene="ileS"
                     /locus_tag="ONT64_000273"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR2301716.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42097..43623)
                     /locus_tag="ONT64_000274"
     CDS             complement(42097..43623)
                     /locus_tag="ONT64_000274"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR2301717.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43638..44285)
                     /locus_tag="ONT64_000275"
     CDS             complement(43638..44285)
                     /locus_tag="ONT64_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCR2301718.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44469..44798)
                     /locus_tag="ONT64_000276"
     CDS             complement(44469..44798)
                     /locus_tag="ONT64_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HCR2301719.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44975..45703
                     /gene="sfsA"
                     /locus_tag="ONT64_000277"
     CDS             44975..45703
                     /gene="sfsA"
                     /locus_tag="ONT64_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR2301720.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45719..46732
                     /locus_tag="ONT64_000278"
     CDS             45719..46732
                     /locus_tag="ONT64_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2301721.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46732..48009
                     /locus_tag="ONT64_000279"
     CDS             46732..48009
                     /locus_tag="ONT64_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR2301722.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48072..50774
                     /locus_tag="ONT64_000280"
     CDS             48072..50774
                     /locus_tag="ONT64_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2301723.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50926..51243
                     /locus_tag="ONT64_000281"
     CDS             50926..51243
                     /locus_tag="ONT64_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2301724.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51273..51593
                     /locus_tag="ONT64_000282"
     CDS             51273..51593
                     /locus_tag="ONT64_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR2301725.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51701..53161
                     /locus_tag="ONT64_000283"
     CDS             51701..53161
                     /locus_tag="ONT64_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2301726.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53229..53450
                     /locus_tag="ONT64_000284"
     CDS             53229..53450
                     /locus_tag="ONT64_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301727.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53481..53663
                     /locus_tag="ONT64_000285"
     CDS             53481..53663
                     /locus_tag="ONT64_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HCR2301728.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53663..54076
                     /locus_tag="ONT64_000286"
     CDS             53663..54076
                     /locus_tag="ONT64_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HCR2301729.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54199..55380
                     /locus_tag="ONT64_000287"
     CDS             54199..55380
                     /locus_tag="ONT64_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2301730.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55451..55615)
                     /locus_tag="ONT64_000288"
     CDS             complement(55451..55615)
                     /locus_tag="ONT64_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301731.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55769..55842
                     /locus_tag="ONT64_000289"
     tRNA            55769..55842
                     /locus_tag="ONT64_000289"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55803..55805,aa:Arg,seq:cct)
     gene            complement(55879..56079)
                     /locus_tag="ONT64_000290"
     CDS             complement(55879..56079)
                     /locus_tag="ONT64_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2301732.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(56384..57616)
                     /locus_tag="ONT64_000291"
     CDS             complement(56384..57616)
                     /locus_tag="ONT64_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR2301733.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            57870..58439
                     /locus_tag="ONT64_000292"
     CDS             57870..58439
                     /locus_tag="ONT64_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2301734.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(58617..59057)
                     /locus_tag="ONT64_000293"
     CDS             complement(58617..59057)
                     /locus_tag="ONT64_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR2301735.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            59215..59979
                     /gene="map"
                     /locus_tag="ONT64_000294"
     CDS             59215..59979
                     /gene="map"
                     /locus_tag="ONT64_000294"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR2301736.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            60011..60934
                     /locus_tag="ONT64_000295"
     CDS             60011..60934
                     /locus_tag="ONT64_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCR2301737.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            61010..62149
                     /locus_tag="ONT64_000296"
     CDS             61010..62149
                     /locus_tag="ONT64_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCR2301738.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            62142..62942
                     /locus_tag="ONT64_000297"
     CDS             62142..62942
                     /locus_tag="ONT64_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR2301739.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            62942..63769
                     /locus_tag="ONT64_000298"
     CDS             62942..63769
                     /locus_tag="ONT64_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR2301740.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            63747..64481
                     /locus_tag="ONT64_000299"
     CDS             63747..64481
                     /locus_tag="ONT64_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR2301741.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            64581..65447
                     /gene="rfbA"
                     /locus_tag="ONT64_000300"
     CDS             64581..65447
                     /gene="rfbA"
                     /locus_tag="ONT64_000300"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR2301742.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            65461..66033
                     /gene="rfbC"
                     /locus_tag="ONT64_000301"
     CDS             65461..66033
                     /gene="rfbC"
                     /locus_tag="ONT64_000301"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR2301743.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            66055..67083
                     /gene="rfbB"
                     /locus_tag="ONT64_000302"
     CDS             66055..67083
                     /gene="rfbB"
                     /locus_tag="ONT64_000302"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR2301744.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            67181..68032
                     /gene="rfbD"
                     /locus_tag="ONT64_000303"
     CDS             67181..68032
                     /gene="rfbD"
                     /locus_tag="ONT64_000303"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR2301745.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            68067..70100
                     /locus_tag="ONT64_000304"
     CDS             68067..70100
                     /locus_tag="ONT64_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301746.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            70144..71424
                     /locus_tag="ONT64_000305"
     CDS             70144..71424
                     /locus_tag="ONT64_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HCR2301747.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            71634..72440
                     /locus_tag="ONT64_000306"
     CDS             71634..72440
                     /locus_tag="ONT64_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2301748.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            72452..73672
                     /locus_tag="ONT64_000307"
     CDS             72452..73672
                     /locus_tag="ONT64_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2301749.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            73662..75248
                     /locus_tag="ONT64_000308"
     CDS             73662..75248
                     /locus_tag="ONT64_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2301750.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            75287..77425
                     /locus_tag="ONT64_000309"
     CDS             75287..77425
                     /locus_tag="ONT64_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR2301751.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(77793..78776)
                     /locus_tag="ONT64_000310"
     CDS             complement(77793..78776)
                     /locus_tag="ONT64_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR2301752.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            79154..80545
                     /locus_tag="ONT64_000311"
     CDS             79154..80545
                     /locus_tag="ONT64_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCR2301753.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            80561..81601
                     /locus_tag="ONT64_000312"
     CDS             80561..81601
                     /locus_tag="ONT64_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR2301754.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            81620..82705
                     /locus_tag="ONT64_000313"
     CDS             81620..82705
                     /locus_tag="ONT64_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HCR2301755.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            82738..>82985
                     /locus_tag="ONT64_000314"
     CDS             82738..>82985
                     /locus_tag="ONT64_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCR2301756.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRAL"
BASE COUNT        27149 a        13958 c        17559 g        24319 t
ORIGIN      
        1 taaagaaaga agttgaaaac gatacctgat tacaaaaaaa taacttttat aagttctata
       61 acgttgatta tatttttaat tcttattctt ctcgtcgtct tggttccccg aatttttggt
      121 ttgactcctt atattattga ggacaaagaa atgagtccat attatgcaca aggaaatctg
      181 atatatgtac gaccgaaaaa tacaaatgag attttagtcg gagatattat tacatattat
      241 gaaaattcag gaaaaaaaat ctcgacgcgt cgcgtaatag ccgttgaaaa caaccacgaa
      301 gatttttata caaaagctga tacaaagact tatattgagc ctggagtagt aaaaaagaga
      361 aatattatcg gtagtcccat ctttcagatt ccatatattg gactagtcct ttcaaaaacg
      421 gcatttgcat ggattcaggg gttattcttt acgattgctt tttgcctaac aggaatcaca
      481 gcatggaata cctttgtaga gttgaaaaag aagcggagac gaacacagaa atttttgtaa
      541 gaatgttaaa aacataaatg tattccagag atccaacgac tcttctgaac atgaaacacg
      601 agaaaacact catgcagctt gtagatttag tcagttgttc gtataatgtg tgaagaggag
      661 gagtagtatg cgcaatgaaa tgatgcatcg tcctgtagtc aaagaagaac tattggattt
      721 tatgcgtacc cagcaaaaaa aattaccggg tgagctaggc aaactggaag aagaagctca
      781 tgtagcagaa gtaccagtta ttccccatga aacagttgta tttttgaaat ttttacttgg
      841 acagcttcag cctgaacgaa tcttggagat cggagcagct atcggttttt catcaagtgt
      901 catggccacg acaatcggag aaaacgctca tgttacgaca attgatcgtt tcgatgtcat
      961 gatcgagaaa gctaaaaaaa actatgaacg tctaggactg acagataaag tgactttgct
     1021 tgaaggtcaa gcagcagata tcttgccgga gttgtctggt ccgtatgact ttatttttat
     1081 ggatagtgca aaatcaaaat atattgaatt tttacctgaa tgcttacgtt tgttaagaaa
     1141 aggtggcgtt ttgatggtag atgacatctt ccaaggagga acgatcttgc tcccagatga
     1201 agagattccc cgtggcaaac gagctatcca tcgtaaatta aatgaatttc ttcgtgttgt
     1261 gatggatcat cccgatttga catcgacaat cttgccatta ggtgatggtg tgatcttaat
     1321 gacaaaggaa tcagaaacaa tcgagttgtg atacatgatt atggcagcct gtagataatc
     1381 gattgaatcg agggctcaat tcgaacttga tgtaaaagtt tctatatttg taagtcaatt
     1441 aaaggataca ggaaatctaa ataaacagga gtagcatgaa gtcaatgtag ctcctgtttt
     1501 caattgataa ccttagaaca gctgaaggag aatgtctcgt tttaaaagat tgatttagta
     1561 gttgggccaa aataattcat tatttgctaa ttaaataact atttcgtatt gacagaagca
     1621 atttaattat ctataattat tgatgttccg ctgattcttg tcatagtagc tcagctggat
     1681 agagcatccg ccttctaagc ggacggtcgg gggttcgaat ccctcctgtg acgctaaaaa
     1741 accgttagaa actgaggttt ctaacggttt ttctttgttt tttgtgccaa tattgtttgt
     1801 tttcaaaggc aaataaaata aaaaaacatc cttatcgagt acaattataa ggagcaaaaa
     1861 aataaaactc atcagaaaag aggaagaaga tatgacgata ctatctagaa ttttagtgac
     1921 tctagttgca attgaatttt tttatatcat gtatatcgaa acggtgagaa ctgattctga
     1981 cacgacaagt agggttttta aaatgagtaa agaagagtta agcagaaaat ctgttcaaac
     2041 attgtttaag aaccaaggag tgtacaatgg actattagga gtgggactac tttatggagc
     2101 ttatctttct tccgcatcta aagaaatcac aagtatgcta ttaatatcta ttttttttgt
     2161 tgcattatat ggcagtttga ctagcgatcg ttggatttta ctcaaacaag gtgggctagc
     2221 tctgttggca ttgctttcta ttttcttata aatatagaaa aaataagaat ttatctaaca
     2281 ggaaactttg tgaaaaatat tactttatct catgaaatat gatattctaa tgaatggtat
     2341 tttagaaaat ttcatgaaaa aggagttaga taggtgttca aaaattatgt ttcctcacta
     2401 aaaaaagaat tttcaggtta tgatctttca agatttcaga aagatctttt ggctgggatc
     2461 acagtagctg ctgtagcatt gcctttagcc ttggcttttg gggtatcaag cggagctgat
     2521 gcggccgcag gtttgattac ggctatcatt gccggtatcg ttattggcgg tttatcaggc
     2581 ggtttttatc aaatatcagg tccgacaggt gcgatggcag ctatcttgat gtctattgcg
     2641 gcaacaaagg gaatgcaagg tgtattgctt gctacttttt tagcgggtct atttttactt
     2701 gcagcaggac ttttccgatt aggaacactg acttctttta tcccagcacc cgtaattacg
     2761 gggtttactt caggtattgc tatcattatt gcattaggtc agatcgataa tttatttggt
     2821 gttcactcgg aaggtacaaa cgttattgaa aaactaagta gttatcaaac gataggattt
     2881 ccgataaaca cagcctcttt gatcatggga gggctagtca ttttagggat gtttatctat
     2941 ccaaaaaaat gggcgaaacg aatgcctagt tcgctgctgg ctatcatttt ggcaacagca
     3001 gttatgctat tgttccattt accagtagca actgtgggta agattccaca aacattgatc
     3061 agtagtaatc gtttgaatat gggagatttt agcttttctg ccttgcaaca agtggcagtt
     3121 ccagctatta gcattgcttt attaggaatg attgaaagct tgctatgtgg tgcttcggct
     3181 ggccgaatgg caaataagcc cttagatagc aatcaagaat taatggcgca aggaattgga
     3241 aatctgttgc ttcctttctt tggcgggatt cctgctactg cggccatcgc ccgtacaagc
     3301 gtggccatca aatctggtgc acaaactaga ttagctggta tgatacatgc agttgtctta
     3361 ctgctctcca tgcttgtttt tgcgccagtg atgtcacaga ttccaatgcc agcacttgca
     3421 ggtgttttga ttgtgacggc ttggcgtatg aatgaatggg aaacgatcaa ggagctattt
     3481 actaataaat attggtcagc agttctattg tttatcctta cgatgatttg tacagttatc
     3541 tttgatttaa gtattgctat cgtgatcggg gtgatcggcg gctgtgtatt ttttgttgtc
     3601 aaaagcgctg ctatcacgat ttcattagaa acgatcgatt ggcaaagaat ggggctgcca
     3661 gaaacggaaa agctgaataa ctggacagta gtgtatatta gtggtccact gttttttatg
     3721 tcttcagaaa aactcaaaaa taaattggaa gaactaaatc atcacgatgg tgtcattttc
     3781 tccatgcgag gcgtaccaag tattgatttg acggcacgtg cagtttttga tgaatttcaa
     3841 gaaaaagcag agcaaagagg acaaacgatt atctatactt ccttgcagcc agaagtagaa
     3901 aaacaacttt ctcacttgtg ggaacataca ttcacgcatc aacatcaaac agttgctcat
     3961 gcgttgacag ctttacatgc gaaatatact gcaattagcc attaaaacaa tgaaaaagaa
     4021 cagacaagac tgtaaactga agattttatg gtcttgtctg ttctttttaa tattgctgta
     4081 aaagtgaagc tagtacttgc cagtcttgat ggataacagg tacaagggag cgattgtaga
     4141 aaatagctgc tggatgaaaa agtggaagaa ttcgatagtt tttttctgac cattcgtagg
     4201 catccttttg ttctgttaat tgtaagacag gccctgtata gagttgacca tgatttttgg
     4261 taaccgtata ttctttgcca agtaaacgtt gaagaccaat gtttccaagt gtcgcaatta
     4321 attttggctg gaccttcatc agttcgtaat ccaatattgg tgcatgcgca aatacttcgg
     4381 aacgagtagg tgtccgattt ggataaacag tttctgactg ttgtgtccgt ttattgatcc
     4441 gatgagtcac gcgatacggg cggctcctta cagcactggt gatatacacc tcttctcgtg
     4501 ttaagccagt tgaagaaagt gcctgcatca attcttttcc agcttgaccg ctaaaaggaa
     4561 catgcgtatc aatctctgtt ttccctgggg cttctcccac gagcatcagt ttaggatgaa
     4621 gaggtccttg cccagcgata aatccttcta caggacggcc gttcattctt aaagaagctt
     4681 cttgtatcag ttcttttggg tattccattt atgctcctcc tttttctttt attataagaa
     4741 taaaagaaac acaatgcgac tattttattt ggtagaactg tgaggataga aaagcagaag
     4801 tattgcttga atttttgttt tactttttga ttttaaattt agttgactaa cggttgctgt
     4861 tctggtaagc tagtactgtt gtaattgtgg actccacagc tacaaccgct cagaacaagt
     4921 aataagcaca gcatatgctg tcacttcgga tggcgagtct aagtatatca gaaggaggtg
     4981 ctttatagca tgtacgcaat tattaaaact ggtggtaaac aagtaaaagt tgaagtggga
     5041 caagcaatct atgttgaaaa acttgacgtt gaagcaggag aaaaagttgt ttttgacgaa
     5101 gttatcttag taggcggaga atctacaaaa gtaggcgctc caactgttgc aggtgcaact
     5161 gttgaaggta ctgttgaaaa acacggcaaa caaaagaaag tcgttacttt caaatacaaa
     5221 cctaaaaaac attcacaccg taaacaaggt catcgtcaac catatacaaa agtggttatc
     5281 gacgcgatca acgcttaata gcgtttgaga aagaaggcct atatatgatc aaaggaacat
     5341 ttaagcgaaa tgacgcaggt cagatcgttt cattcacttt gaccgggcat gctgatgctg
     5401 gtccttatgg aagtgatatc gtttgtgcgg gtgtttctgc gttagcgatc agcactgtca
     5461 acgggattgc ctcattagcc ggttttgagc cgattgttga aatgaatgaa gaagaaggcg
     5521 gttatcttta taccgaagtc acttcaggaa tgacacaaga acaaaacaat attgctgaga
     5581 ttctattaga aaatctttta ttaggattac aatcgataga agctgaaaac tcagaatata
     5641 tccaaattaa aacaattaat gataaatagg aggtgcagac catgttgtta tctatgaatc
     5701 tacaattatt tgcccacaaa aaaggtggcg gttctacatc taacggccgt gactcagaat
     5761 caaaacgtct aggtgctaaa cgtgctgatg gacaaacagt tacaggtgga tcaattcttt
     5821 accgtcaacg cggtacaaaa atctatccag gtgtaaacgt gggtatcggt ggagacgaca
     5881 ctttatttgc aaaagttgat ggcgtagtac gtttcgaacg taaaggccgt aacaaaaaac
     5941 aagtgtctgt ttatccagta gctcaagaag cttaattgaa tcttatgatt aaagaggttg
     6001 tgacaagact tttgtcacag ccttttttgt ttataaaaat aaaagttgta gtggaggcta
     6061 cgaagtttac tttgtctttt tacacttaat atttttttgc caatttggaa tcaggatgtt
     6121 ttgataacta tcgttttcaa ttgatggaag ctatattgtt tgctatcagt gaaataatta
     6181 tatggtaaac agtgttcttt ttcttgtttc cattataaaa atcgtatcgg gatttttcgt
     6241 tcttttaaat tgaacatttc tatctatccg cgtgttatca acaaaacaaa atcaggtaaa
     6301 tagttgacga aacagaaata aatacatagt attatgtttt gaagcctaag tacaatagtc
     6361 tatagaaaag gagcgatacc attggcaaaa cgcattgacc gaatttatat gtatgtcaaa
     6421 gaaaaaaccg ctcatttaac aaaggcagaa tatgatcaag gagtaacaac acaggaaatt
     6481 gctgaagtgt tagggattca gcgaacaaat agcagtaaag atctgaatca gctagtgcgc
     6541 gaaggaaaac tgataaagac agacggacga ccagtacgtt acatatatca aacactcgtt
     6601 acgcacgtaa agccagttac aaaacatgta gtcagttaca aagaaacgcc ggtctctgaa
     6661 gaaaaaaata cactccaaat agatactaag gatatctttg caaaaatcat tggagctaac
     6721 ggcagcatga aaaattcagt ggaacaggca aaagctgcga ttttgtatcc gccaagagga
     6781 ttaaattgtt tgattacagg acctaccgga tcaggaaaga cttattttgc acacgcgatg
     6841 tatcattttg caaaagcgca tcatgtaata gatgaggaaa cggaactgat tgttttcaat
     6901 tgtgctgatt atgccaataa ccctgagctt ttgatgagtc atttatttgg gtacgcaaaa
     6961 ggtgctttta caggcgcaga tgaagaaaaa atggggatta tcgatcaagc ggatggcggt
     7021 atgctgtttt tggatgagat tcaccggttg ccgcctgaag gtcaagaaat gatcttttac
     7081 tttatggatc atggaactta cagtcgtctt ggggaaacaa cgaaatccca tgaagcaaat
     7141 gtacggatcg taggtgcgac cacagaagac ccaggttctt ctctattaga aacttttgtt
     7201 cggcgtatcc caatcaatat caaacttccg gcttttgaaa aacgtcctgc aaatgagaaa
     7261 gtcgatttag taaaaatcat gattgcccac gaagcaaacc gcacgcagcg aaagatttct
     7321 ttgacagaag atgtagcaaa agcattgatc ggcagtgtga cctatggaaa tataggacaa
     7381 ttgaagtcaa atatccagtt agtatgtgca cgaggatttc taaatcatat gcaatcaccc
     7441 gaaatctcga tcacaatcga tgatttgaca gaagggattc gtagcggatt gattcaatta
     7501 gcaagcaacc gcagtgcaat ggcagagctt tctaaaatat tggagccaaa gataactgta
     7561 gcaccaaatg atacgatcat gaaaatccaa tcggactcct atgagttacc ttataatcta
     7621 tatgatatca tcggagataa agcagcttta ctgaagtcgg atggtttgga tcaagaagca
     7681 atcaatcatt tcatttctac agatatcaac atccatctga agtctttcta taaagaccat
     7741 ggtttttctt ttaatgcaga cagcaaatta gcagaatttg tagatccgaa aatcatagaa
     7801 gtgaccaatc agatttatgt aatggtcaaa agatctttac catatgagtt tcagcaaaac
     7861 tttatttatg ctatgagctt gcatatcagt tcttttttga aaaaaatcaa cttaggagaa
     7921 gaacggcata cgaatgataa catccgtgag atggcttttg attttccaga tgaatatgct
     7981 gttgcgaaag aagtgcgtag ttatatcgaa acgtattttc aagtggagat tccagaaagt
     8041 gaagactatt acttgaccgt tctactcgtc tcgttacggg caaatcaagc aagcggcaga
     8101 atcggtgtag tcgttgcagc tcacggaaac agcacagcaa gcagtatggt ccaagttgtc
     8161 caacagttgt tagatgcaga gaacgtacga gcagtagaca tgcctttgga tatggatccg
     8221 aaaacagcat tggaccgaat tgaacgcaat gtacaagaag tagatgaagg aagcggggtc
     8281 atactcttag tagatatggg gtcccttgcg tcgtttaatt cgcaaattca aagagacact
     8341 ggaattccag tacgtactgt tgatatggta acaacttctc tagttttgga aactgtacga
     8401 aaggtctcag tattagggac agatctagat atgttgcatg aatcattgaa aaattttcga
     8461 ggttacgcag aaatatcttc tgtagaatct gttcaggaag caaaacctat tcataagaaa
     8521 aaagctgttt tggctatctg cgcatctggc gaaggtacag cacaaagaat caaagaattg
     8581 atcgaacgag cggtaaacaa gcgtcaagaa acagaattag aagttctagc tttgtcgatt
     8641 gtggaagtga aagaagaact gccagttatc caaaaagaat atcagatcat agctactact
     8701 gggattactg atttgaatat ccaagcccca tttattcctt tagaacgttt tattgatcaa
     8761 aatatcgaag tcattttgga tcagttgctg atggaatcag aattggagga aactgaattc
     8821 atctccctag atgaagagtc agctaaaaat acgtgtgttg agtttatctc tgataacttc
     8881 atctttatta atggtagtaa gttaatcgat ccaatgtggc aattttctac acaaatctct
     8941 cagacgacgg gaatcggcga tgaagaatat ggattcaaaa tcaacctagt gatgcacaca
     9001 gcaggaatga tcgaacgtat tattcggaat gaaccattga ctgtggaaga aaacgaattg
     9061 acaaatacaa cgaatgatcc tctttatagc caattggcag cctcagttgt tctgttggaa
     9121 gatcagataa aagtaaaagt gccgatagaa gaaatgtatt atctgctacg tctagtccat
     9181 aatcagttag ataaaaaaga atacactgtg ccatgaatac acaaaaacga tacactttta
     9241 tagtgtatcg tttttgtgta ttggttaaga atcctttaaa ttcaattagt gtatcgtaaa
     9301 aaagttggca cgctttttgc ttatagtagt gtgtgctagg aggtggttgt tcacacagaa
     9361 atgatagggg tttctgtgaa gtagtgagga agaaggagtt ttgcagatgg atattcgatt
     9421 ggcacgtata gatgatcgtt taatccacgg gcaagtcgca acagtttggt cgaagcaaac
     9481 aggtgttgaa cgtatcattg tgatcagcga tgtagtcgct caagataaat tgagaaagtt
     9541 tctactaaaa gaagctgcac cgccggggat cacagcaaat gtaataaccg tgcagaaaat
     9601 gattcaaatc tatcatagtg gattattatc acgagcaaaa gtgatgcttt tattcaccaa
     9661 tccccaagat gttgaagcgg ttgtccgctg cggtgtacag ttgaagtcgt tgaatatcgg
     9721 cggtatgagt tttactgaag gcaaatcaat gattaccaat tttgtttcag taaatcaaca
     9781 agatattgat tcctttcgtt acttgaacag tcaagaagtg gagttagaga ttcgaaaagt
     9841 acctgctgac aggaaaatcc ttttgatgga actactagcg aaaatcaaaa aggtgtagca
     9901 agaatggttg ttggaaaagg tcaaataatt ctgttgtaat ggttagcaat tattaatatt
     9961 tttgaatttg ttacaacaaa agtaaatagg aggtataaat atggtaggga ttatcctagc
    10021 aagtcatgga caatttgctg aaggaatctt acaatccggt tcaatgatct ttggcgaaca
    10081 agaaaacgtc aaagccgtga ttttgaaacc aagtgaaggt ccagatgact tgagagccaa
    10141 attggaagaa gcggttgctt cttttgataa tcaagacgaa gtgttattct tagttgattt
    10201 atggggcgga acaccgttca atcaatcaaa cacattattc gaagagcaca aagataaatg
    10261 ggcaatcgtc agcggtttga atttaccaat gttgattgaa gcctacgcat cacgcttttc
    10321 aatggaatct gctcatgaaa ttgcagcaca catcattgaa acagcaaaag acggtgtaaa
    10381 agtaaaaccg gaagaattag aaccggcaga agcaccgaaa gcagcagttg aagacgcaca
    10441 acctaaagga gcacttccag aaggaactgt ggtcggagac ggaaaaatca aatatgtatt
    10501 agcacgtgtt gactcacgtc tgttacatgg acaagttgcg acagcttgga caaaagcagt
    10561 gcaaccaaac cgtattatcg ttgtttcaga tgcagtatca aaggatgatc tgcgtaagag
    10621 actaatcgaa caagctgctc ctccaggtgt aaaagcaaat gttattccta ttagcaaaat
    10681 gatcgaagta gcaaaagacc ctcgttttgg aaataccaaa gcattgcttt tatttgaaaa
    10741 tcctgaagac gtattgacag ctgttgaagg tggcgtagac atcaaagaat taaatgttgg
    10801 gtcaatggcg cattcggtcg gtaaagtcgt tgtaagtaaa gtattgtcta tgggacaaga
    10861 agacgttgaa gcgtttgaga agcttgaaca aaaaggtgtg aaattcgatg tacgtaaagt
    10921 acctaatgat tcacgcgata acatggatga tattttgaaa aaagcaaaag ctgaattagc
    10981 aaaagcgtaa taaataatat taaaatttat aggaggctta tcatgtctat tatttcaata
    11041 attttagtcg tattagttgc ctttctagct ggtatggaag gtattcttga tgaatttcaa
    11101 tttcatcaac cactagtggc atgtacattg atcggtttag ttaccggtaa cctcgaagct
    11161 ggtatcgtac ttggcggtac gcttcagatg atcgctttag gttgggcaaa tatcggtgcc
    11221 gctgtagcac cagatgctgc attagcatca gtagcatcag caattatttt agttttaggt
    11281 ggacaaggtg ttaaaggtgt tccttctgcg atcgcgattg ccgttccttt ggctgttgca
    11341 gggttgttct taacaatgat cgtacgtaca atcgctgttc caatcgttca tttgatggat
    11401 gcggctgctg aagaaggaaa catccgtaag gttgaaatgt ggcacatcat tgctgtatgt
    11461 ttacaagggg ttcgtatcgc ggtcccagca gcagcgttgc tatttatccc agcacaaact
    11521 gtgcaatcat tccttgaatc aatgccagca tggttaacag atggtatggc tatcggtggg
    11581 ggaatggtcg tagctgtagg ttacgctttg gtaatcaaca tgatggctac aaaagaagta
    11641 tggccattct tcgttattgg tttcgttgta gctgcaattt ctcaattaac attgattgct
    11701 ctaggtgctt taggtgttgc attagctctt atttacttga acctttctaa aatgggtggt
    11761 tcatcaaata atggaggcgg aagcaactct ggtgaccctt taggcgacat tttgaatgac
    11821 tattaatctt gaaggaggaa aataaaatgg cagaagaaaa aatcaaatta tcaaaaaaag
    11881 atcgtttagc tgttgcgtgg cgctctacct tcattcaagg ttcatggaac tacgaacgta
    11941 tgcaaaacgg tggctgggca ttttctatga ttccagcaat caaaaaatta tacaaaacaa
    12001 aagaagaacg ttctgcagca ttaaaacgtc acttggaatt ctttaataca cacccataca
    12061 ttgcttctcc aattttaggg gttacattgg ctctggaaga agaacgtgcg aatggcgcgc
    12121 ctgttgatga tgtagcgatt caaggggtta aagtaggtat gatgggtcct ttagctggtg
    12181 ttggtgaccc agtgttctgg tttacagtac gtccaatgct aggtgcacta ggtgcttcat
    12241 tagctatggg aggaaatatc cttggaccaa tcatcttctt cttaggttgg aacattattc
    12301 gttgggcatt catgtggtat acacaagaat ttggctacaa agctggttct aaaattactg
    12361 atgacctttc tggcggattg ttacaagacg taacaaaagg tgcttcaatt ttagggatgt
    12421 tcgtattagc tgcccttgta caaagatggg tatcaatcaa attcttacca gttgtatcaa
    12481 ctgtaaaatt agataaaggt gcttacatcg aatgggataa attgccttca ggtggtgaag
    12541 gaatcaagag tgcatttgaa caagttaata atggcttagc cctttctcct gaaaaggtaa
    12601 caacattaca agacaactta gaccaattga ttcctggttt agctgcatta ggattaacat
    12661 tgttatgtat gtggttgctt aagaaaaagg tttctccaat cgttatcatc cttggtttat
    12721 tcgtagtcgg tgttgttgga cacgtcatcg gacttttata atccataaaa atatagctcg
    12781 ggcgttgtcc cgagcttttt ttatatagta tactgttcat aaacaaatat ctggagggag
    12841 aaaatggttc aatcaatcaa tacaaaagta gacttggtaa ttgatgctac ttcgtttacg
    12901 ggtctgacag attacgggca aatcatgata ggtgataatg gttttgagtt ttaccatgcg
    12961 cgagatagac gcaaatatat ccaaattcca tgggaagaag tggattatgt actagcatca
    13021 gtcatgctaa agggaaaatg gattcctcga tatgccattc aaacgaaaaa aaatggtaca
    13081 tacacatttt cttccaaaga acctaaaaaa gtgcttcgtg cgatcagaga atatgtagat
    13141 gaagatagaa tggtacaatc attgagtttc tttgatgtag tgaaacgtgg tattcgtagt
    13201 atgtttaaac gcaagtaaaa aataaagaaa acttctaaga ggaggttttc tttatttttt
    13261 tactttgcag aagaaaatat gaaatagcaa aaccaattat ttgtcccctt taaatcccat
    13321 atgctaaaat tgattagcaa agaaggaggt tatattatga gttggttatg gacattgatt
    13381 gttggagccg tgatcggagc aattgcaggt gcgatcacca gtaaaggaaa ttcgatgggc
    13441 tgtgtaacga atatcattgc tggactagtc ggttcaagta ttggacaagc gattttcggc
    13501 gaatggggac caaaagctgc aggtatggca cttattccat ctattttagg cgcagtgatt
    13561 ttggtagcag ttgtctcatt tttctttgga aaacgatcat aagtttttac aaaatagtaa
    13621 actaaaagga gttgcagcga gactgtgact cctttttggc tttatttatt taaaataaaa
    13681 agaagttttc cggaaatttt tatttaactg tcgtgtacga agaaaagttt gctataatgg
    13741 aaaaaagcgt gcaaagaaag agggagtttc attatgatgc tacgtgtaga aaaattgcgc
    13801 aaaaaaatgc aagaagaaaa tttggattca tttttggtta ccagtccgta caatcttcgt
    13861 tacttaacga actttacagg tacgacagga ttagctgtta ttactttgga aaaagcattt
    13921 tttattactg attttcgtta taccgagcag gcggcagcgc aggctcaagg gtttgaaatt
    13981 attaaaaatg tcggacctat ttttgatgag gtagcaaact tagtacgcaa agaagaactg
    14041 cactcattag cttttgagga aacaacagtc tcatttttag aatattcggt tttagaagaa
    14101 atcatcgatg cggaattagt acctgtttca cagatgatcg aagaactcag agaagtgaaa
    14161 gatgaagaag aaatcgctat tatcgaaaaa gcatgtcata ttgcggatat ggcatatgat
    14221 cacatcctga aaatgatcca accgggcatg actgagattg aagtcgcaaa tcaactagac
    14281 ttttatatgc gttctcttgg cgcttcaggt gtttcttttg atacgatcgt tgctagcgga
    14341 ttgcgttcgg ctatgccgca tggtgtagca agcaaaaaaa tcatcgaaca aggtgacttg
    14401 atcacattgg actttggttg ctattacgaa gggtacgttt ccgatatgac acgtacattc
    14461 gctattggtg atcctgggga aaaattaaaa gaaatttatc aaatagtctt agatgctcaa
    14521 ttagcagtta ttgatgctgc aaaaccagga atgactggtg tccaattaga tgcagttgct
    14581 agagattata tttcaaaaca tggttacggg gaagcatttg gacattctac aggacatggg
    14641 atcggtttag agatccacga aggaccaaac gtttcttctc gtgcagaaaa gcaatttgta
    14701 ccaggaaatg tgatcactga tgaaccaggg atctacttac caggaatcgg tggagtacgt
    14761 attgaggatg acttgttgat cacagaagat ggaaatcgtg tgttgactcg ttcaccaaaa
    14821 gaattgatca tcttatagtt tcatcaatag aagaagtaag cgtaaaaatc agtgttcaca
    14881 gactttgtta tagaaaatca ctcgatttaa tgatacacta tagaagaact tttaagctgt
    14941 aagagataat aaagttgggt gttttctcca attcatagca gtttgcagct gatcggcggc
    15001 agttttctta ccacgtgatc actatgccgc atcatagaga attcggatgt atctcgtgct
    15061 taggcaaagg gaggaataaa taaatggcag atgaaaaaaa tttgatttta aacgcaaacc
    15121 aagatttagg agagatcgtg atcgctcctg aagtcatcga agtaattatc gggattgcag
    15181 cttctaaagt agaaggcgta tacggaatgc gcggaacttt tgccagcaat gtcactgaat
    15241 tattaggacg tgcagctcac ggaaaaggtg tttacttagc aaacgaggaa gatggcttga
    15301 aagtagatct ttactgttat ctagaatatg gtgtttcagt tcctaaagta gcaatggaaa
    15361 tgcaggaacg ggtcagccag caagtccttt tcatgacgga tatcgattta tctgaagtca
    15421 atatccatgt agtagctgtt gttcctgaaa aattagaaca accaacaatc gaagaacttt
    15481 ttgaagacga agaggaagag aatgaataaa gaattatcaa gacacgagat tcgggaaatg
    15541 gcattgcagg ctttatttcc attagacttc aatgcagacc taacaaaaga agatgcgatt
    15601 ttcaacgcaa tcgaattaga ccaccgcgac atgatcaacg aagatgaatc agagtttgtt
    15661 cctgtttatc ttgatacgct agttggcggt gtctgtgcaa aaaaagatga attggataaa
    15721 gtgattgaaa aacatttaaa aaacaattgg cacatcaatc ggatctcaaa aatggatttg
    15781 gtgatcttgc gtatcgcgat ttttgaaatg acttatgtga cagatgtacc agcggctgtt
    15841 gctttaaatg aagcaatcga gttggcaaaa acatttagtg atgaccgttc acgtaaattc
    15901 gtcaacggtg ttttatccaa tgtattgaaa gaattagaag ctggggcgta agtcctagct
    15961 tttttctatg aatcatcata aacctgttat aaatcgggaa aattctatgg tagaatgaaa
    16021 acacaaaaaa tatctgggag tggttagatg gcagaattga ttaacggaaa agaattagct
    16081 gaaaaaatgc aggcagagat tgctgaaaaa atccaaaaat tgaaagataa tggtatccat
    16141 cccggattag tcgttttatt ggtaggggaa aatccagcca gccaaatcta tgtaagaaat
    16201 aaagaacgtg tagcaaaaga aatcggaatc cattcattgg tagagcgtta tcctgagacg
    16261 atttctgaag acgaactact tgcagaaatt gaaaaataca atcaagatcc tagttttcat
    16321 ggtatattgg tccaattacc attgccaaag catatagatg aagaaaagat cctgttagct
    16381 atccgccctg aaaaagacgt agacggtttt catccgatga atttaggcag actccttgcg
    16441 ggtaaaccgg acaagattcc ttgtactcca tacggcatca tgaaaatgtt tgaagcttat
    16501 tctatcgatc cggcgggtaa acgagcagta gttatcggac gaagcaatat cgtcggtaaa
    16561 ccgatggctc agctactctt gatggcagat gcaactgtta ctgttgctca ttctaaaacc
    16621 gaaaatctgg ctgaactggc acaagaagca gatattttag tcgtagcaat aggacgtgga
    16681 cattttgtca caaaagaatt cgtaaaacca ggtgctgtag tgatagatgt agggatgaat
    16741 cgtgatgcta acggtaagct gatcggtgat gtgaaatttg atgaagtgga gcctattgca
    16801 ggctggatca ctcctgtgcc aaagggtgta gggccaatga ctatcacgat gctgatgtat
    16861 caaaccgtaa aatctgccga agcttttgga gcaggtgaat agatggcaca agaatattta
    16921 actgtcacta cattaacgaa gtatttgaaa agaaaatttg atgccgatcc ttatttagaa
    16981 cgcgtatatt taacaggcga aatatcgaat ttccgtttac gtgccaatgc ccatcaatat
    17041 ttcagcttaa aagatgacca tgcaaaaata tctgctatta tgttcaaagg agcattccaa
    17101 aaattacgat tccagccaaa agaaggaatg aaagtccttg tcgttgggcg tatttcatta
    17161 tatgaagcgg gaggttctta tcaaatctat gtagagcaca tggagcctga tggaataggt
    17221 gctttatatc aagaattgga agaacgaaaa gaaaagctga tgaaggaagg cctttttcaa
    17281 gggcctaaaa aaatgctgcc acgtttccca aagcgaatcg cggtccttac cagtccaagc
    17341 ggagcggtta tccgcgatat catcacgacg gtgaaaagaa gatatccaat tgtccaattg
    17401 gttttgttcc caacagttgt acaaggaaac caagcagcgg acaatgttgt caggaatatc
    17461 cagagagtag aagagatggg caatttcgat acaatgatca tcggtcgtgg gggtggttcg
    17521 attgaggatt tatggccgtt taatgaagag cgggttgctc gggcgattta tcaagctaat
    17581 acgccggtga tttcatctgt aggacatgaa acagatacca cgatcgctga tttagtagca
    17641 gatgttcggg ctgccactcc aacagcagct gcagaattag ctgttcctgt tttgtcagaa
    17701 gagttgatgc gcattcaaga acggcaagct agattgcagc aggcgtttgt acgccagatc
    17761 cagcgcaaac aagaacggtt cgagcgtgct cgacgttcct atgtatttcg gcagcctgaa
    17821 agattgtatg aaggtcagac catcaaatta gaccagttga aacagcgatt atatcaagct
    17881 acacagcaaa tttatcatgc gaaagaaaaa caaacgatcg cattaagcca ccaattggaa
    17941 caagtggctc cgctttatcg agttaaagca gctcgccaag aaactgctta tctagaaaaa
    18001 cgcttagaag agaaaatcca acaatatatg aaacaacagc agcagcgttt tgctcaagct
    18061 gttcaatctc ttgatttgtt gagtccattg aaaatcatgg gaagaggcta cagctatacg
    18121 acgttaaatg atcaagtagt aaaaagtgtc accgagattc atcaagggga tttgctgacg
    18181 atccattatc aagatggaat cgttaaagga caagtacaag agatagacaa aggagaataa
    18241 aaatatggaa aatccaactt ttgaagagtc actgcaagaa ttagaaaaaa ttgtcatgca
    18301 gttagaacaa ggagacgttc cattagaatc agctctagat tcgtttaaac gaggaatgga
    18361 attaagcaaa cattgccaag atacattagc taaagcagaa gagaccttga cgaaaatgat
    18421 gacagaatca aatgaagaag ttgattttga cggaaacgag gaaagctgag atgagattaa
    18481 ctgacttttc agctattcat ctacctgctg tagaaaaaga aatcttgtct tttttagatg
    18541 agcatacaac ggatagagga ctctatgatg cgatgagtta ttcagtgaaa gcaggcggga
    18601 aaagaatacg tcccttatta cttttaacgg ccgttgcttc ctttgatgag cctattgatg
    18661 ttcccgtcta tcaagtagca gctgctttag aaatggtgca tacgtattcc ttgatccacg
    18721 atgatttgcc tgcgatggac aatgatgatc ttcgtagagg aaaacctacc aatcataaag
    18781 tattcggcga agcattagct atattggccg gagatggttt gcttacagga gcattccagc
    18841 taatcagtat ggcacactta ggtaattctc ctaagcttct gctgcttcag caattagctg
    18901 tttgtgcggg atcacaaggt atggttgctg ggcaagctgc agatatcgag ggcgaatcga
    18961 aaaagctttc tttagaagaa ttagcattta ttcatgaaag aaagacaggg catttgattc
    19021 gttatgcctt gctggctggt ggcatattag cgaagcaacc tgaagaaatc ttattgctac
    19081 ttcaacgatt agccgaacat ttaggtttag cttttcagat aagagatgat ctactcgatg
    19141 tgattggtac aacgaaaaca ttagggaaaa cagctggtaa agatgaacgt atggaaaaaa
    19201 atacttaccc gcgtttatta ggcctagaaa agacacgtga ggcgttggag attgagctgt
    19261 tatccgccaa caagatcata gacaaattag aagaggacgt tttttctttt gatggcgggc
    19321 tgatgcgcca gatgatcaag caatttgatg tggagaaatg agggaaagaa tggaaaaaga
    19381 acgcgtagat gtattagcgg tcaaacaagg attatttgaa acaagagaac aagccaaacg
    19441 ttcagtgatg gctggtttga tttacaacga aaaaaacgag cggtttgata aaccgggaga
    19501 aaaaattcca gtgagtagtg aattaagagt caaaggtaaa aaacttcctt atgtttctcg
    19561 agggggatta aaattagaaa aagcactcaa agcatttgat ctatctgttg aaggaaaaac
    19621 attgttagat attggtgcat ccactggcgg atttactgat gcagctttgc aaaatggcgc
    19681 aaaaatgagt tatgctttag acgttggcta caatcagcta gcttggaaaa taagacaaga
    19741 tcctagagtc gtcgtaatgg aaagagtgaa ttttcgttat gcaaaacccg aagactttac
    19801 cttaggaatt ccagaagttg ctgttatcga tgtttctttt atttcactaa aactgatgct
    19861 gcctccatta catgctattt taaaagaaga gggagaagtg attgctttaa tcaaacccca
    19921 attcgaagca ggcagagaag ctgtagggaa aaacggaatt gttcgtgacc ctcagaccca
    19981 tcaaaaagtt ttagaagaca ttttgtcatt tgcggcacaa ggtggatatg atgtgctgga
    20041 attaagttat tctccgatca cggggggaga aggaaatatc gagttcttgg ctcacttgag
    20101 gaaagtaccc gaaagcggaa caatcaattc agctattaat atggcagaag tggtttcaaa
    20161 tgcacacgaa cagtttgacc ataaataaat tgtaactgtg aaaatcaagt gagaagtatg
    20221 cggattcttc tttattatac caaggaaata agctatcatt gattaagaag aatatgctaa
    20281 ggagcgaaga cgtaatgaga aaaaaagatc ggcatcgttt gattactcga ttgttgagtg
    20341 agcaagatat ccgaaagcag gaagaatttg ttgaaatcct gaaaagcaaa ggaatatctg
    20401 ttactcaagc tacgatttcc agagatataa aggaactgaa actgatcaag gtaccggctt
    20461 cagatggcgg ctaccgttat agcataccag ctgaaacaaa cgaagatgta aatactaaat
    20521 tggaaaaact attaaaagac gctttcgtgg cagttgacca aatggaaaaa tttgttattt
    20581 tgaaaacatt gccgggaaat gcttcggcag cagcaaattt gattgataaa cgttacaaga
    20641 aagaattatt ttcgattatc aatgatgacg ataatgtttt gatgattaca cgaactgaag
    20701 accaggcagt tttattgaag aaagacttcc tgcattacct ttaagttgag gagcgaatgt
    20761 gatgctacaa gaaatcagta ttactaactt tgcgattatt ccggaattgc ggctttcttt
    20821 tcacgaagga atgaccgcgc tgaccggaga gactggtgcg ggtaaatcca ttattatcga
    20881 tgctttaggt ctgttagcag gaggaagagg ttcaagcgat tatatacgtc aaggagcaga
    20941 aaaatgtgtg cttgaagggt tgtttgaact accaaaacaa gaaggattct ctgaactgat
    21001 ggttgaatta gggatcgaaa cagatgagga caatctgatt gttcgacggg atatgtcctt
    21061 aactggaaaa aatgtttgtc gagtgaatgg tcatattatc acattagcta atctgagaag
    21121 aatcggcagt tatttagtag atatccaagg acaaaacgaa catcaggaac tgctccagcc
    21181 tgaatcacat cttgctttat tggatcgttt tggagatgta gctttccaac agaagaaaaa
    21241 gagttatcag caagaatata tctcttatcg tgagctggaa aaaagggtac gaaaagtgca
    21301 gcaaaatgaa aaatcctatg ttcagagaat agatatgctc catttccagc aagaagaaat
    21361 agctgctgct gatttgcaag tcggagaaga agagaaatta aaagaagaaa gagaaaaact
    21421 tagcaattat caaaaaattg ctgatggatt agctgcaggt tacggagcac tgacgaataa
    21481 tgaacaaaac agtgtagatg gtgttggctt ggccgtttca gaactgcaag ggatcgccca
    21541 tctagatgtg gagtatgaag caatttatga aaatatccaa agtgcttact atttgctgca
    21601 agatgcaatc ggcgatatga gtcgtcagat tgatttattg gagttagatg agagtcgttt
    21661 agaagaagtt acgcaacgtt tagaattaat ccgacagttg aaacgtaaat acggcgaatc
    21721 cattgagtca atattggctt actatgacga aatcaccgaa gagctagctt cttctgattt
    21781 ttcagaaggt caattggaca aaatgaaaga ggaactagag caaaaagagc ttctacttca
    21841 gcaacaagcc gcagatcttc atgaagcgcg taaaaaaatc gcaaaagaat tggaaaaatc
    21901 cattctgcat gaattgaaaa gtctgtatat ggaaaacact gagtttgagg tacgtttctt
    21961 aaaagaagaa aatagacaat tgaatcatga tggatttgac caaatcgaat tttacattac
    22021 gaccaatcca ggagagccac tgaagccttt ggtgaaagtc gcttctggtg gcgaactgtc
    22081 tcggatgttg cttgctttga aaactatttt ttcttctgag caaggtgtga caagtattat
    22141 ttttgatgaa gtggatacag gtgtgagcgg aagagttgca caggccatcg cagataaaat
    22201 ttctcaaata tcgaaatact ctcaagtttt gtgcatcact catctgcctc aagttgctgc
    22261 agtcgccgac tatcaatatt atattgtgaa agcagtgatt gatggacgta cgcaaacttc
    22321 tgtttcagag ttgaaaacaa aagaaagaga agaagaaatt gcacgtatgc tggctggaag
    22381 tgagattaca aaactcacac tcgaacatgc aaaagaacta ttgaaactcg caaaaaggac
    22441 ctgagccacc tcgcagtggt tcaggtcctt ttcagcagct gatagctata ttttattttt
    22501 acatgacact cattacagca gtcaatacaa cagatccaat cgttaaaatc agcattgtcc
    22561 aaatgacaaa ttttgttact ttagcaaaag tacttgtttg ttttttttca cgcattgttt
    22621 tcaacctcgt cattaattag ttgattcaag tgtacaatct tttttccatt tccacaagag
    22681 ggctttttta taaaaacttc ccttttttta aataataggt agttacgata caaataaata
    22741 atgttaatag aactagccat ataaaggcat catccctttc agcaataggt aacttaatgt
    22801 tcattccata gatactgccg acgattgtcg gtatagttaa aataatcgtt aaagaagtta
    22861 aaattttcat aatattattc agattattcg atacgattgc tgaaaaagtc tgattcattt
    22921 gttctactag ttttaattga atgcgagaag tagtcattgc ctgctctgtt tccaccaata
    22981 catcacgtaa atgatttttc aaagcatgat tttcttggaa aagtttattt tgcgctagtt
    23041 ctttcaatgc atctaaatta gcagaagtcg cttctctgaa ataaacgagg cttttttgga
    23101 tatccatgat ttgataaagt tgtttgtttt cagtggatac ttgaagtttg gcttccaaag
    23161 tatcgcattg agattgaagc gtagctaaat aactgttgta agagagtgtg acctgccaaa
    23221 tgaattgaaa gaagatattg agattaggcg aaatatcact ttttggaaag gtctggctaa
    23281 agactttctt taaaaacagt ggttcatgat tcgtaattgt gataagcttc tttttgggcg
    23341 tcaaaataat agagagcggg tatgtatcca cttgaaaata accgctagga cttgttttag
    23401 caaaaggata ttgtaacaat aataacgctg gctttgtcaa ctttgattga ttgaattctt
    23461 ctgtacggga attttctgca tcgtccaata tggaagtgat gtaatctgac ggaatttcaa
    23521 attgatgtgt cagctgatga atctcttctt gattaggacg ttctacacaa taccagatcg
    23581 tttggtcagt ttcctttccg gtattacgca gtttctcatt atctaatgta aaatatttga
    23641 tcatgatttt cctcctgttg cctgtttctt ttattataac ggatagggaa ttggaataca
    23701 tggaatgtgt ttgtaagata tagaatataa ctgcataaat ttaaataaaa tgaaacagtt
    23761 gtgtaaagta tatacagtat acttgaagca acgataaaaa aggagtttac gttcatgggc
    23821 acattcacac cacttatctt gttttggtat atttacccgg taatcgtttt gtttggttgc
    23881 caatttcttg taaaattatt ttcgttaaat cgtcgtttta aaataaaagc ccctgatttg
    23941 gcgattcctt ttttgtgggg cggtatgcat gcattgtcta gaaatacagg gacaatttct
    24001 atcttaccgt ttttctttat ttctgttttg ctaatgggaa tttgtattgc tgttttccaa
    24061 gcatattatt atgaagaaat tatttatccg cgttatttca agatgacttg gcgtttggtt
    24121 tttctgttga caatcgtctt gtacgctgtc ttgatcattg tgaacatact ttcttaccta
    24181 tgaaaaaaat agaaaagctg taaaaagtct tttaaaaggc tttttgcagc ttgattttta
    24241 aaaaaatgtt tatgaataaa aaaagttgtt cccggatttc ccagaaaagg tggtagaaag
    24301 tgggggattg tggtagacta attttagtta gtggaggaat ggggggcttt caatgttgat
    24361 gggtgaattc caacataata ttgatgcaaa aggacgttta atcgtgccct caaaacttcg
    24421 tgaagaactt ggcgaaaagt ttgttctgac acgcggattg gatggctgtc ttttcggcta
    24481 tccgatgagc gaatgggaaa atctggaagc taaactgaac gaaatgccgt tggctaaaaa
    24541 agacgcacgt acttttgtgc gattcttcta ttcggctgcc actgaatgcg aacttgataa
    24601 acaaggacga atcaatatac ccagcacgtt aagaaattat gcagctttga caaaaggttg
    24661 cgtagtcatc ggtgtatcta accgaattga aatttgggat gagacacgat ggcaagattt
    24721 ttctgcagca gcagaagaga actttgacga aattgctgaa agtatgattg atttcggact
    24781 ataagaatag gaagaggctg actaatgact gaaacatttc aacactatac agtcatgttg
    24841 aaagaaacgg tagatggatt gaacatcaaa ccggatggcg tttatgtaga ctgtacactc
    24901 ggcggtgccg gacatagtga atatttatta agccaactaa gttctaaagg gcatctgtat
    24961 gcgtttgacc aagatcaaaa agcaatcgat cacgccaagc tgcgtttagc ttcttatgtc
    25021 gaacaaggta tggtcacatt tatcaaagcg aacttcagag aattagaaga acgcttgagt
    25081 gagcatgtag ataaggtaga cggcattttg tatgatctag gggtttcctc tcctcagcta
    25141 gatgaagcag aacgtggatt tagttatcat caagatgcac cgttggatat gcgcatggat
    25201 cagtcagctc ctttatccgc ttatcacgta attaatgaat attcctacca tgaattagta
    25261 aagattttct tccgctatgg cgaggagaaa ttttccaaac aaattgctcg tgaaatcgaa
    25321 cgagtacgaa aaacaaaacc aatccaaaca actggtgagc ttgtcgaaat cattaaacaa
    25381 gtcatcccag ctcccgcaag aagaaaaggc gggcatcctg caaaaagaat ttttcaagct
    25441 gtgcggattg cagtaaatga cgaactcggt gccgaagagg cctctttaga acaggcaatc
    25501 cggttattga aagtcaatgg aagaatcagc gtaatcacat tccattcatt ggaagacaga
    25561 attgtcaaaa gtatgtttaa agaatatagt acggtacaag atctaccacc gggacttcct
    25621 gtcatacctg acgaattcca accagaactg aaagtcatta ctcgtaaacc aatccttcct
    25681 tcagaggaag agctaacaga aaacaatcga tcacgcagtg caaagttacg tattgcagaa
    25741 aaaataagaa tgaaagagga gtagaatgat ggcagaatta aagaaaatgc aagattacga
    25801 atatgattta cctgaattgg tggatcagcc ggaacaacaa atgcctgatt cttcttcgaa
    25861 tcaagaaatc attcttccac aatcaccggc aaaacgatta aagcagattt ctgctttgga
    25921 aaaaattgct gtggcaagcg tggcgattgc tattgttgct ttatgtgttt taacagttat
    25981 gctgcgcaca aatatcagcg gagctgaaaa agaaatcacg tcaatacaaa cagaaatcaa
    26041 tgataaaaag caagaaaaaa cgagtttaga acaagaaaaa aacgaacttt ctcgtactga
    26101 acggattaaa caaatcgcag aagaaaaagg attgtctatt aatgacgaca atttaaggaa
    26161 agtgaagtaa atgagtctta aaaataaaat tcggcggttt atggaaaaga aaaatctaaa
    26221 tccgatgaac aatcgcaaaa aagtaggtat cattttattt gctacgagta ttggattgtt
    26281 ctttttattt gccgtccgtt tctcgtatat cgttattgga ggtcatgttg ctggtacctc
    26341 attagctgaa aaaacaaaac agttgtacca aggaagtgaa gtagtaaaag caaaaagagg
    26401 aacgatttat gatcgtaacg gtgtggcgct cgcagaagat gcctcctctt attcaattaa
    26461 agcgatactc tcaaaaacat atacctctgg ggataaaaaa ctgtatgttg aagaaaagaa
    26521 ttttgataaa atagcagaaa tcctgcataa gaatctttct atagacaaaa aagatgcact
    26581 gaatatttta gaggacggcg caaaaaaaga gctgtatcaa gttgaatttg gtagctatgg
    26641 gaaaaatatc tcccaagaaa ccaagcaaaa catcgaagcg gacatgaaaa aagaaggtgt
    26701 cgctggtctt tattttgtcg accatcaggc tcggatgtat ccaaatggcg tcttttcttc
    26761 tcattttatt ggatacgcag ttccagacaa agatgagaat ggcttagttg gtaaactagg
    26821 tttggagtct gcttacaatg atatcttgag cggtaaagat ggtaagatta tttatcaaaa
    26881 agataatttt caaaatcctc tgccaggtac agtggcagaa gaagaaaaag ctgtcgacgg
    26941 acaagatatc tacacgactt tagacagcag actacaaagt tatttagaaa cactgatgga
    27001 tcaagtaaat gaagaatatc agccagaaga attgacagca gtcctgatga aagcaaaaac
    27061 cggagaaatc ttggcaatgg gacaacgacc tactttcaat cctgaaacaa tggaaggatt
    27121 gactggaaaa gatgctatct ggcgtaattt cttggtacaa gatagttatg aaccaggatc
    27181 aaccatgaaa gttttcacta ctgcagctgc tatcgaagaa ggagaattca acgagaatga
    27241 aaccttccaa tctggaaaaa tccaagtaga agatgctacg atcaatgacc atgactttgg
    27301 tgaaaaaggt gtgctgacca tgcgtcaagc tctttcatgg tcaagtaacg tcgggatggt
    27361 catactggaa cagcgattag gcggacgatg gtataactat ttacaaaaac tgggatttgg
    27421 acaaagtact cattctggat tagatgatga agtaaacggt gctttaccta cattaaatat
    27481 tgtcgaccga gcaatgagtg cttatggaca agcggtaggt gtgacgaatt tccaaatgat
    27541 gaaagctttc acttctattg cgaataacgg tacaatgatt caacctcgat atatcagcaa
    27601 agtggtggat ccccaaactg gagaagagcg aacaactcaa acagaagttc tagggcaacc
    27661 tttttcaaaa gaaacaacag aaaaagtccg tgaatatatg agagatgttg tcgaaagcga
    27721 aaactacggt agtgcttatg gtgtatatag tgtacctgga tacaatgtat cggcaaaaaa
    27781 cggaacagcc caaatcgctt ctgatacagg gggatatcaa acaggagata cagcctacct
    27841 gtattcgatt gtggagatgg ttccttctga agatccagac tatgtcttgt atctaacaat
    27901 gaaacaccct aagacatatg atcgaatggc attggctaaa atcgcaaatc cattgatgaa
    27961 acgggcaatg gattttaaag aaactgaaga agattcagat acagaaacaa aaacagaaaa
    28021 agtatccgta gccgattatc gtaatcttga agcggacgtt gcagcagctg atgctcaaaa
    28081 aagtggactg caacctgtag tgatcggtaa tggtaaaaaa gtacaaaaac aatctactgc
    28141 caacggtgat cagttgatct ctggagaaaa actgatccta tatactggtg gtgataagct
    28201 gatgcctgac gtaacaggat ggtcaaaagc agatatcatg aagctgggga aaatcctcgg
    28261 tattgaagtt agttttgatg gagatggtta ttgtgtcaaa caagaacttg ctccatatga
    28321 aaaaatcact gaagacaaac ttagttttac tttagaagaa taagaagaga aataggagag
    28381 aaaagagcat ggaatggaca caagcattga ttcccattgt cagcagttgt gcaatgacaa
    28441 ttgccgcaat gccgctattt attggttatt ttcaaatgaa aaagcaaggt caggcgattc
    28501 gtgaggaggg accaaagtgg cacaatagca aagccggaac tcctacaatg ggcggcttag
    28561 tgtttctgat cggctctatt ttgacaggaa tttgggttgg agcttggcaa aaacagctaa
    28621 ctcctactct tttcatcttg ctttttgtat tagcgttata cggagtaatc ggctttcttg
    28681 atgattttat aaaaatattc aagaaaagaa acatgggact gaactcgaag cagaaattga
    28741 ttggacagat tctaggggga atcatctttt atcttgttta ccgttctgaa ggttatccag
    28801 gtgtattgaa tttcttcgga ctagatctac ctttaggaat catttatggt attttcgctc
    28861 tcttctggct tgttggattt tcaaatgcag taaatctgac agatggcatc gatggattag
    28921 ttgctggctt aggaagtatc tcttttgcag cttacggtct aattgcttgg catcagcatc
    28981 agtatgatgt attaatcatt tgcttaagtg ttttaggagg tttactgggt ttcttcgtct
    29041 acaacagaaa acctgccaag atttttatgg gagatgtcgg ttcattagcg ttgggcggac
    29101 ttttagcggc aatttctata atgctaaacc aagaatggac attgcttttg attggtttga
    29161 tctatgttat ggaaacagca agtgtgatgc ttcaagttac ttcctttaag cttacaggaa
    29221 aacgaatttt taaaatgtct ccaattcatc atcactttga aatgtgcgga tggtcggagt
    29281 ggaagattga tacagttttt tggttgatca gcattgtgac ttcgttgatc acattatgga
    29341 tcgtttggta ggagatgacg ggaaatgaaa aaaatatcaa catatgaaaa taagaaagtg
    29401 cttgtcttag gactcgcaaa aagcggagta agtgcggcca aattattaca tgaattgggc
    29461 gcacttgtca ccgttaatga tggaaaacct tttgacgaaa atccggaagc acaagagctg
    29521 ctttctttag ggataaaagt aataacaggt agtcatccaa tcgaattgtt ggatgaagaa
    29581 ttttcattga tggtcaaaaa tccagggatt ccctacagtc atcctttagt agcaaaagcc
    29641 caagaaatgg gtattccggt aattacggaa gttgagctag cttacgaagt agcggaatgc
    29701 ccaattattg ggattacagg aacgaacgga aaaacaacga caacaacgat gacaggacta
    29761 ctactgaatg caggagcaga tcaaggaatt gcacgtttag cgggtaatat tgggtatcca
    29821 gcaagtggtg tagcgcaaga agcaaaatca gaagataaaa ttgtgatgga attgtctagc
    29881 ttccaactca tgggaatcac agatttccgc cctcatatag cggtgattac caatatctat
    29941 gaagcacata ttgattatca tggtacaaga aaagaatatg tcaaagctaa atggaacctt
    30001 caaaaaaaca tgacagaaaa agattatctg atccttaact ggaatcagtc agaactacaa
    30061 gaattagcgc aaagaacaaa agcacgcgta ctgcctttct cgacaaaaga agtgttggaa
    30121 gacggtgtgt atgctgacga ttactcaata tactataaaa aagaaaaaat aatggaaata
    30181 tctgaacttg gcgtaccagg taagcataat gtagaaaatg cactagctgc tatttctgta
    30241 gcaaaattgt atggtatatc aaatgaagct atacgagaaa cgttgcattt tttccatggc
    30301 gtaccacatc gtacgcaata tgtaggagaa atccaaggca gaaaatttta taatgattca
    30361 aaagcaacca atattttagc gactaaaatg gctctaagtg gatttgaaac gtctaaggtc
    30421 gtgttattgg ctggcggatt agacagagga aatacatttg atgaattaat tccttcatta
    30481 aaaggaatca aagcaatggt cgtatttggc caaacaaaag aaaagttgat ggatgctgga
    30541 aaaaaagcag gcatcgagac aatagtaaca gccgattctg tagaacaagc agttccttta
    30601 gcattagaaa acagtacaga tggtgatgta gttttattat caccagcgaa tgccagctgg
    30661 gatcaatacc caaattttga aactagagga aacagattca tggaagctgt gaaccgatta
    30721 aaatagaaaa agtagaagcg agcgataaaa aatgaaaatt ttggttacag gcggaggaac
    30781 agggggacat atttatccag ctcttgcctt tgtcaattat gtaaagactc aagaaccgaa
    30841 tgctgaattc atgtatgtgg gagcaaaacg aggtctagaa aacaaaatcg tccctgatac
    30901 aggaatgcct ttccatacct tggagattca aggatttaaa cgaaaaattt ccatgcataa
    30961 catcaagacg gtccgattgt ttcttaagag tatccgtcaa gcaaaaaaaa tcctcagtga
    31021 ttttcaacca gatatcgtta tcggtactgg aggatatgtt tccggagcgg tagtgtacgc
    31081 tgcagccaaa atgggtattc caacagttat ccatgaacaa aacagtgtac caggtgttac
    31141 caataaattt ttaagccgat atgtggatcg gatcgctctt tcatttaaag atgcggcttc
    31201 cttttttcct gaaaacaaag cagttcttgt aggtaatcct cgagcacaag aagtagcaga
    31261 tactaaaaaa tcagatattt tgaaaacatt tgatttggat ccagaaaaga aaacagtctt
    31321 gatttttggc ggtagtcaag gagcacttaa aatcaatcaa gcagtatcag tttttttaag
    31381 tgaatatatt ccttcttctt accaagtttt gtatgcttca ggagatagat attatgaaga
    31441 gatcctaaat aaaataaatc ctgaaataca aaatattagt gtccagcctt atatcaaaaa
    31501 tatggcagag gtcatggctt ccagtgatct gctggtgggg cgtgctggtg caacttcgat
    31561 cgcagaattc acagctctgg ggttaccggc agtgttggtt cccagcccat atgttacgaa
    31621 tgatcatcag actaaaaatg caatgagtct tgttcacgca ggagcagcaa aaatgattgc
    31681 agataatgaa ctaacaggtg aatcactaag tcagacagtc aacgaaatca tgggtgatga
    31741 agaattgcaa aagcaaatgt gccgagcttc taaagaacaa ggaatccctg atgcttcaaa
    31801 acgtctttat gacttagtaa aacaaataat caaaaaataa aggcggtggt cttagtgagt
    31861 aagaaaaaag aaccaaaaaa attgtctcat ccgtcttctg atgaaaacaa tttgactcct
    31921 tggcaaaagg ctaaccgaaa atatctggca gaacaagaga tgaaaaacca agagccaaaa
    31981 aaagaagaca taacattaga tacacccctt tcagattctg aagaagaaaa aacggttcaa
    32041 gaggaacagc aagaagaaag ccagtccgag aaaaaaatcg aattttttga agagatcaag
    32101 caaaagaaaa acggtggacc atataacggt tcttttttga accggctgcc caatttaaaa
    32161 agtcaacggc acaaagtact atttcgtcgg ttagctttga tcatcactgt attgggtata
    32221 ccgctgattt ttttgatcta ttatgtttct ccttatagca aattacaagc agttgcagta
    32281 tcaggcaata agacagtcaa cagccaagaa atcatctctg atacaaaatt gtcattagga
    32341 gaaaatgttt ggggacagta ttttcatcga tctacttata tcgatcgcct taaaaaagca
    32401 cagccaagaa tcgaaacagc aaatatacat ttcaaaggga tgaatgaatt cgatttggat
    32461 gtaacagaat ataaagagat tgctttgatc gcaaaaaaca atcaatacta cccagtgatt
    32521 gaaaatggaa cagtattaga tgaaaaagta gcaaatccta caaaaaatct ccctatcttg
    32581 gaagagttca aagactctgc aaaaatcaaa gaattaacaa agcaatacaa tcagttatca
    32641 tcagaattac aaaaagcaat ttctgaaatc aagtacacgc caagagcaag caataaaaat
    32701 ctgatccaat tgaatatgaa tgacggcaat caagttattg taaatatcaa taatcttgcg
    32761 aaccaaatga aatattattc gcaagtagcc aaagatatgg atgaaaaagg tgtgatcgac
    32821 atggaagttg gtatcttctc gcatccttac ggtagcactc aagaaacgga agaatcgggc
    32881 caatcggaag aaacaactga aagtgaagaa aatgcggatc aaacaaacga atcaagtgaa
    32941 gaggcagaaa cgcaaaaagc acaaacacct ggatcgtcta gcacaaacgg atcatctgat
    33001 tcaaatgatt caagtgaaac aagtcaaata gaagataatc caagtacaac tccgtcaagt
    33061 ttaaatgaaa attgataaaa caactttaaa aagtaagaat gtactttcta aaaaccagta
    33121 atttatggta gaattggaag cagtgaatat atctattaat aagtatattt taaaacggac
    33181 gctagttgtt cgcttgtata tggaatagta agtaggaggg aatcccattc atggcaaaaa
    33241 caggaatgta tgtaggcctt gatattggaa cgacatctgt taaagttgtc gtggctgaat
    33301 atatcgacag ccaaatgaat attattggag taggaaacgc aaaatcagaa gggattaacc
    33361 gaggcattat cgttgatatc gataaaacgg tccaagcgat acaacgagca gtacgacaag
    33421 cagaagaaaa agcaggtatc cagatcaaag gcgtaagtgt cggtttgcca gcaaacatgc
    33481 ttgaagtaga aaattgccag ggaatgattg cagttaacgg agattcaaag gaaatcaccg
    33541 atgaagatgt cagaaatgtg gcttcagctg cccttgttcg ttcaatacct cctgaacgtc
    33601 aaatcgtgtc aatattgcca caagatttta ctgtcgatgg gttcgaggga atcaaagatc
    33661 ctcgtggaat gatcggcgta agattagaaa tgtacggtct gttgtttaca ggacctaaaa
    33721 cgatcatcca taacatacgt aaatgcgtgg aaaatgccgg attaatcgtc aacgaaatgg
    33781 tgattactcc tttagcgctg actgaatcaa ttctttctga cggagaaaaa gattttggta
    33841 cgatcgtcat tgatatgggt ggcggccaaa caacaacggc tgttatgcat gataagcaat
    33901 tgaagtttac caatcttgac caagaaggtg gcgaatttgt cactaaagat atctctattg
    33961 tattaaatac gtcatttaac aatgcagaag cgttgaaaat aaactacggg gatgcatacc
    34021 cagaacggac atcaccagat gaagaatttc cagtagatgt gattggtcag tcagaaccag
    34081 tgaaagtcga tgaacgctac ttgtcagaaa ttatttcagc tcgaatggaa cagattttca
    34141 acaaagcaaa agatgcattg gaccaaatcg aagcattaga gttgccaggt ggcgtcgtat
    34201 tgacaggcgg agctgctagt cttcctggtg ttgtggactt ggcacaagaa atcttcggcg
    34261 taaatgtaaa attgtatgtt ccaaatcaca tggggctgag aaacccggta tttacaaatg
    34321 taataagtat cgtggattat tcagcaaact tgagtgaagt gtatcaattg gcaaaaattg
    34381 ctgttacggg tgaaacctca gcagctcgtc aaatggtagt tgaacaagaa acaacaaaca
    34441 catatgagag ctatgaagca cctgaagaaa caatctacga tgagccggaa ccaaaagagt
    34501 ccggagaaaa tgtaaaaaat aaaatcaagg gtttctttac aaatattttt gattaattca
    34561 tcctgggagg aataagatat ggaattctca attgataata atatcaatga cggcgcagta
    34621 atcaaagtca tcggagtcgg tggtggcggt ggaaatgctg taaaccgaat gattgaagaa
    34681 aacgttaaag gtgttgaatt cattacagcc aacacagacg tgcaagcatt aaaaaattca
    34741 aaagctgaaa cagttatcca attaggcccc aaatatactc gtggtttagg tgctggttct
    34801 cagccagaag ttggacaaaa agcggcagaa gaaagtgaac aatccttacg cgaagcttta
    34861 gacggtgcag atatgatttt catcactgca ggaatgggtg gaggaaccgg tacaggtgct
    34921 gcaccaatcg ttgcaggtat cgcgaaagaa ttgggcgcat tgactgttgg tgtcgtgaca
    34981 cgtccattca cttttgaagg accgaaacgt ggccgttttg ctgcagaagg tatcgctcgt
    35041 ttgaaagaaa atgtggatac attattaatt atctcaaata accgtttatt agaagttgta
    35101 gataaaaaga caccgatgct tgaagctttc cgtgaagcag ataatgtttt acgacaaggt
    35161 gtacaaggta tctctgattt gatcacagcg ccaggttacg ttaaccttga ctttgctgac
    35221 gtgaaaacag tgatggaaaa ccaaggtaca gcattgatgg ggatcggtgt ggctagtgga
    35281 gaagatcgcg tagtagaagc aacgaaaaaa gcaatctcct ctccattgct tgaaacatct
    35341 attgatggag ctgaacaagt acttcttaac atcacaggcg gtttggatat gacattgttc
    35401 gaagcacaag atgcttcaga tatcgtagca aatgcagcaa ctggcgatgt aaatatcatt
    35461 ctaggtacat ctatcaatga agaaatgggc gatgaaatcc gtgtgacggt catcgcaaca
    35521 ggtatcgatg aatcaaaaaa ggaacgcaag tctagccgac cagctagaca agcacaaatg
    35581 caatcacctg ctcaaaagac tgtattagat atggatcaag ctaaaccaac ttcttcagaa
    35641 gaagaaaaca gttttggcga ttgggatatc agacgtgaac aaaatgttcg tcctagagtt
    35701 gatgattcca attttgacaa tatcgaaaaa aaagaatttg atacattcaa ccgcgaagaa
    35761 acaaaatctc atggcgatga tgaattaaat acgccaccgt ttttccgtcg taaaaaataa
    35821 aggagaggaa gagcacatga ttgctgacaa cttgcagaaa atcaagcaag aaattcagga
    35881 ttcgtgtgct cttgttcatc gtgatccgaa agaagttaca ttagtagctg taacaaaatc
    35941 tgttgaatcc gctcaagctg aagaattggt tagagctggc gttatgaatt gtgcagaaaa
    36001 ccgagtagat aaattattag aaaagaaaca agatttattt gattttcctg aaataaagtg
    36061 gcatttaatt ggtaatttac agcgcagaaa ggtaaaattg attgtaaatg aaattgatta
    36121 ctttcatgcg ttggacagtt taagattagc agaagaaatc cagaagcggt tagagaaaga
    36181 attatcctgt tttattgaag taaatgtttc tggtgaggaa tcaaaacatg gaatttcacc
    36241 agatgagctt ttaccgtttg tggaatcttt agctgcctgc acaaaaatca aggttgttgg
    36301 gttgatgact atggcgccta aagaagcttc tgatgagcaa atccataaag tgtttggtac
    36361 gctgaaagaa ttgcgtgatc ttgttcaagc aaaaggttat gcccatgcac catgtagtga
    36421 attaagtatg gggatgagtc aagactttca aattgcgatt gaagaaggtg caacatttat
    36481 ccgtgtagga acagcgctat tcaaagacga ataaaggagg aacaaaaatg ccgcttttaa
    36541 acagaggagc aattgctaat tttttcggat tagctgatga ggaagagtac gaataccaag
    36601 attatcctga aacaacaaaa aaggctgtac agccaactaa aaaaaataca acacagaatc
    36661 aatcaactca ttctgtatct gctgcgaata ctcatgaaaa acaaaaaacg gcgacttcac
    36721 gtcctgtaac gcatgaaagt attcagccgg ctgaaaagaa aaaagtaatt gaactacatc
    36781 aatcacctac aaaaactgta gcgaaagaaa gaacagcttc aagaacctct acacaaccta
    36841 aagcaaatct tgctggaaaa atcactgtaa tggaaccaag agcttattct gaagctatgg
    36901 cgattgctaa acgaatcatt gctggagaag caaccttagt gaattttcat ttagtagaag
    36961 aaaaacaagc gagaagaatc gttgattttc taacaggaac agtttatgca ctagatggag
    37021 atatcaaacg tgttggagat gagatttttc tttgtacacc gactgggatg gagattgaca
    37081 gttcaaccgc tcaatctttt gcagatgcga atttgtttga tctgtaagag gaggaactac
    37141 gatttatact attgcactat tacttcattt acttcaacgg gcagcctatt tctatagcat
    37201 tatcttgatg gtatatgcat tgctctcttg gtttccaggc gggtatcaat cagggctagg
    37261 acgattatta gctaagatct gcgaaccgta tatcagtttg ttcgatcgtc tgcctttgag
    37321 gattggaccc ttggatttca caattttagg ggcaatcctg atcttgaact tagccgtaca
    37381 aggtttagca cagatcgtta tatggataac tgtccattta gtttattgat tgaagcaaca
    37441 aaatctttat tctcagaata gataggaggg gaaaattttg gatgcaaatg tataccagca
    37501 ctttcgggca gatgaacgta catttatcga ttcggtaggc gattggattg aacaggttca
    37561 aggacaatat gcaccctatc tgaccgaatt tcttgaccca agacaatcct atattttaga
    37621 aacactgatt cgacaaagca gtgatttgcg tttcatgttt tatggcggat atgaacaggc
    37681 agaaagaaaa agatgtttga ttttccccga ttattacgaa cctgtagagg aagattttga
    37741 aacagcattg tatgaaatcc attatcctag caaatttgcc agcctaagtc atgggaaaat
    37801 ccttggaact ttagtgggaa cggggatcaa acgctcttac tttggcgata tcatctcgga
    37861 cggtgatcgc tggcaagtat ttattgcaaa tgaggtggat cattttgttg ctgcacaagt
    37921 aacaaagatt ggaaaggttg ctgtacgctt ggaaccaatc aaatacacag aaatgattat
    37981 gccgaaagat agctggacac aagaaagagg aacagttagt tccatgcgac tagacagtgt
    38041 gatctctgag atttataata tttccagaca gcggtccaag cagctgatcg aagctggtaa
    38101 agtaaaagtg aactggacag aaaatacaag acctgatttt gtacttgaac tattagatat
    38161 tatttcagtc agaggttatg gcagagtcca gatccaagaa cttgagggga agacgaaaaa
    38221 agaaaagtac agggtactgt tcggtgtatt acgtaaataa aataaaaagg tggtttactc
    38281 atggctttaa ctccattaga tatccaaaat aagacattcc cgacaaaaat gcggggatat
    38341 aatcaagacg aagtagatga tttcttagat ttagttgttc gtgactatga ggaattgact
    38401 caaagaaatc gagaattaga aaaagcagtc aaacattctg aagaaaaact tgagtacttc
    38461 aatgaattaa aagatgcttt gaaccaatca atcatcgtag ctcaagatac ggctgataaa
    38521 gtaaaaacaa gtgcaagcaa ggaatccgaa gtgatcgtta cttctgcaca aaacaaagca
    38581 gatgagttag ttgcgaacgc tgaaaaacgt gcacatcaat taacaactga tgcagaagaa
    38641 aaagcaagaa aaatcttgac agatgcaaca gaaaaagcaa gacagcttgc gacagaaact
    38701 gaagatctta agaaaaaaac acgagtattc catcaacgta tcagcttgat gcttgagtct
    38761 caattagaac aagtgaaaag tccggaatgg gatgaaattt tacaaccatt ctccagctac
    38821 gtgactgaca gccatgaagt gatcaaagaa gttttagcaa aacagcttga caatgaaaat
    38881 gatactgatg taaactcaga cacaggagtc atagaagcac ctatgacggc atttgataca
    38941 caggctattg atatctcgat cgtgcctggt gaaaatgaaa acgagtagaa cagaaaagca
    39001 ctttgttatt cttaaagcga gccgatgaga gtggaagttc ggtaagacac tgcatggtga
    39061 aagatcctct acttgagacc tgcttttccg aaattagagt aagaagcagc ggctgacctc
    39121 gttattggtc acagagagtt tgcactagat agatgcaact aaatttgggt ggtaccacgg
    39181 catgttcgtc ccttgcggct gaacatgctt ttttgtgtcc ggaaaacaaa taaaggagtg
    39241 tatggttgaa tgaaaatgaa agaaaccttg caattaggaa aaacaaaatt ccctatgcgt
    39301 ggtaatttgc caaaccgaga aggcgagtgg caaaaagaat gggaagaaaa tcgaatctac
    39361 caaaaaagac aagagttgaa cgaaggaaaa cctagctttg ttcttcatga tggacctcca
    39421 tatgccaatg gtaatatcca tttaggacat tcattgaata aaatcagtaa agacattatc
    39481 attcgttcaa aatcaatgtc tggctatcgt gcaccatatg ttccaggatg ggatacacat
    39541 ggattgccga ttgaacaagt attggctaat aaaggcgtga aacgtaaaga aatgtccatg
    39601 gcagaatact tggaaaaatg tcgtgaatat gcactttccc aagtagataa gcaacgagca
    39661 gatttcaaac gcctaggagt tgcaggagat tgggaacatc cttatgttac attagatcct
    39721 tcttatgaag ctgcacaagt acgcgtattt ggtaaaatgg ctgaaaaagg ctatatttac
    39781 aaaggattga aaccaattta ctggtctccg tcaagtgagt cttctttagc ggaagctgaa
    39841 attgaatata aagatgtaaa atctccatct atttttgtag ctttcaatgt caaagacggc
    39901 aaagatattc ttgatgaaga tacagctttt gtcatttgga cgacaacacc atggacattg
    39961 ccagcaaacc aaggtattgc tgtaaatcca aaatttacgt atgtattggt ggaagccgat
    40021 ggccgtaagt ttgtcgtagc gaaagatttg ctagaaacag tccaatcaga aatcggttgg
    40081 gaaaatgtaa cagttctaaa agaatttgct ggacaagaaa tggaatatat gactgcaagt
    40141 catccattct atgaacgtga atcacttgtg attttaggcg atcatgttac tttagacgct
    40201 ggtactggat tagtccatac tgctccagga catggggaag atgactatat cgctggtagc
    40261 aaatataaac tatctgtagt gtcacctgta gactcaaaag gattgtttac agaagaagca
    40321 cctggatttg aaggtatctt ttatgacaaa gcaaatccta tgatcactga tctattaaaa
    40381 gaaaaaggag ctttgctgaa gctagatttc tttactcata gttatcctca cgattggcgg
    40441 acgaaaaagc ctgtgatcta tcgggcaact ccacaatggt ttgcatctat cgataaattc
    40501 cgtcagaata ttttggatga agtagaaaaa gtagagtggg ttatcccatg gggtaaaaca
    40561 cgtttgtata atatgatccg tgaccgcggc gattgggtaa tttcccgtca gcgtgcatgg
    40621 ggagtaccac ttccgatttt ctatgctgaa aatggagaag caatcatcac accagaaaca
    40681 atcgaacatg tggcaaaatt atttgctgaa catggatcaa ttatctggtt tgaaaaagaa
    40741 gcgaaagaac ttcttcctga aggatttacc cacccaggtt ctcctaatgg cgaatttaca
    40801 aaagaaaaag atatcatgga tgtctggttt gattctggtt cttctcatga agctgtttta
    40861 cgtcagcgac cagaattatc attcccagca gatatgtatt tagaaggatc agaccaatat
    40921 cgtggctggt tcaactcaag tatcacgact agtgtggcta tcaatggtgt agcaccatac
    40981 aaatcagtta tttctcaagg atttgcttta gatggtgaag gccgtaagat gagtaaatct
    41041 ttaggaaaca tcattgcacc agaaaaagtg atcaaacaat ttggtgcaga tatccttcgc
    41101 ttatgggtat ctagtgtaga ttatgaagca gatgttcgtg tttctatgga tatcttaagt
    41161 caagtggcag aagtatatcg taaaatcaga aatacgatgc gtttcttatt agcgaataca
    41221 gaagatttcg atcctaaaaa agatactgtt tcgtacaatg atttacgttc tgtagacaaa
    41281 tatatgaccg ttcgattgaa ccaagtaatc aaagaaatcc gagaagaagg gtacgacaaa
    41341 tataacttca tgcatattta ccgtacagtt atgaacttct tgacggttga tctatcgtca
    41401 ttctacctgg attttgctaa agatgtagtt tatattgaag cagaagatga ttatcaacgt
    41461 cgttgcatgc aaactgtttt ctatcaaaca gctgtagctt tgacaaaatt attgactcct
    41521 atcattccgc atacagcaga agaaatctgg tcattcctga aagaagaaga ggagtacgtt
    41581 cagttaagcg agtttcctgg ttatgaagag tttgcaaacc aagaagaatt gatggatacc
    41641 tggactgctt tcatggactt ccgtgataat gtcttaaaag cattggaaga agcaagaaac
    41701 agcaaattga ttggtaaatc tttggaagca aaactaactg tttatccaaa acaacaagtt
    41761 cgtgaaatgt taaaagcttt ggatgcagat attgcacaat tattgatcgt ttcaccagac
    41821 tactttgaag ttatggcagc agatgaacaa gtgccagata acgctatggt atttgatgat
    41881 gttgctatta cagtggaaaa agcagacggg gaaacttgcg atcgctgtcg ccaagttcgt
    41941 aaagatgtag gagtagatga aaaacttcct catctatgcg gtcgttgtgc aaaaatcgtt
    42001 gaagacttct atcctgaagc tgtagctgaa ggattcgaag aaaagtaaaa gggaaaaggg
    42061 ggtgtgacat gttctttgtc acaccccctt ttgttatcaa tcgtttaatt ttccacgttc
    42121 acgataccag ttatctggtt gccagatcca ttcgaatccg tctttttcaa gtagttcgaa
    42181 agctgcttta ggtcccattg atccagcttt gtagttggga aaatccggtt gtgtttggtc
    42241 ccaagctttt cgaatgacat ccacgatatg ccaagattga gcgacttctt cccaatgaga
    42301 gaagttcgtt ccatcaccat tcaacgcatc taatagtaat ttttcgtaag cttctgggct
    42361 attttcaacc atttctgcac tatttctaaa ttccaatttg attggttccg tttcaaatcc
    42421 ttgccctact tctttcccat tcaaggataa agaaaatcct tcagttggct gaatatagat
    42481 cgttagaaca ttaggtggaa gtgtcgtatc gtcacatggt tcatcgacag atgttttgaa
    42541 tacattaaca ggaacttgtt tgaatacgat gttgatccga gtcccttttt cagtcaaacg
    42601 tttgcctgta cgtacataga aaggaacacc tgaccaacgg aagttatcga tagtgaattt
    42661 gcctgcgacg aatgtttcag tctctgattg agcatccact ttgtcttctt cgcgatagcc
    42721 cttgaattgt acaccatcca attcaccttg agcatattgc ccgcgaacga agttttctaa
    42781 cacttcttct tcgttataaa tgcggatagc tttcaatgct ttgatctttt ctgtacggat
    42841 ttctttttcg gaaaatgcta ctggcggttc cattgcaagt aatgctacaa cctgtaaaat
    42901 atgattttgc accatgtctt taagtgcgcc actatgatcg tagtagcccc cgcgatcttc
    42961 tacaccgagg ttttcgccaa aagtaatttg gatattgtcg atatagcgat tgttccattg
    43021 agcctcaaaa atattattgg caaaacggat agctgagatg ttttggatca tttcttttcc
    43081 taaataatga tcaatcctga aaatatcttg ttctgggaat acttctctga tttgctggtt
    43141 caattcaaaa gcagactggt aatcagaacc gaacggtttt tctatgatca aacgattgaa
    43201 gccatttttt gtcataatat tttgtgattt caaatgagaa acgatcgttc caaaaaattg
    43261 aggtgccata gccaaataat agacacggtt accgtttaat tgatagtgtc catctaatcg
    43321 gtcagacaat tcttttaatg tctgataatg cgttgaatca tttacatcat gggattgata
    43381 gtaaaaatgg cttgcaaagg tagttgcttc ttcatctgaa ggattcaatg cacggattgt
    43441 ttctttgaca acttcacggt agtgctcatc gctccaggga cggcgagcgg ttccgattac
    43501 agcaaaatgc tcgcctaaat ttccttttct gtaaaggcgg aacaaagacg ggtacaattt
    43561 acgctgggct aggtcacctg taccgccgaa tatcgtaaac aatacatttt tttcattaga
    43621 catgtggata cactccttta tattttatcg acaaaaatcg tgctggccgc cttaaagctg
    43681 acggggattt tctttctatc attttggata gtgatcggtc cttcataagc tgcgatgtca
    43741 acgatctcat attcttcgtt gatattcagg tcaatagtga ctaagtaatc caacaattct
    43801 ttttcatcaa gcactcgtgc gatacgcact tttgtaccga caggatagga ctccaatgtt
    43861 tgtcggcgtt cttcatggac aacttcctct tttttaggaa tcgatcctcc atgaggacaa
    43921 tgttcaggat gattcaaata gttttctaaa tgatctgcaa gatgttccga agtcacatgt
    43981 tccaatactt cagcatcatc atggacttca ttccagctat agtctaaatg ttcaactaaa
    44041 aaaacttccc ataagcgatg tttgcgaatc agactgctag ctttttgcag tccgcttgca
    44101 gtcaattgta caccttgata aggggaatgt tctacaagct cttctttcac aagtttagaa
    44161 atcatttcac tgacagatgc agctgagaca tctagactag atacgatttg tttattgttg
    44221 actttcgttg tatctccacc tagttctaaa atgattttca aataatcttc tcgatttggt
    44281 gtcatattcg atcatccttt catggtaaaa aaccatcatt agtaagtgta acaaaaaaaa
    44341 cgttaattga ctatttttct aaataaaaaa caaaggattt ttagacgttt ttacaacaaa
    44401 aaacgaaaga tgatagagaa gactcattct ttcgtttata aaggatatat atatacattg
    44461 ggagggatct atttttcaga taacaactta tatgaagtgt ctgcagattc gtcataaaga
    44521 gtttgttcgg gataagaaaa gataagataa aaaccatact gatcacgtac gactaaacga
    44581 tattcatcta tttctaccac agatgtgcca agagactgac cgttgatcaa aaacgtgtaa
    44641 tcagatgtaa tctgtaattg ttgtttttgc tggctatcac taaagaacca tgtaccttga
    44701 tactttgaga gtccttctga cttttccgct tgttttttct ttttcaagta aataaaactt
    44761 gagagaacgg taatgagtga accaagaaaa tagatcattt tttttatcac caaatcactc
    44821 ctttatgaga aaataatcgc atagacatat tctttcttct ttatttcatt gtaccgaata
    44881 aaagctaaaa tttctctaag actttcgact ggttttttta ctgtttttct acaaattatg
    44941 ataggataca tagtaggaag aaaggtgaca taatttgaag tatcagcaag tggaaatagc
    45001 tttttttatt gaccgcccga atcggtttat agcacattgt atgaaaaaag acggagaaat
    45061 aattattgcg catgtcaaaa atacaggtag aggaaaagag gtcctgatac caggcgctga
    45121 agtggctatt gtatttgctc ctggacctaa acggaaaacc gcctatgatc tgattgctgt
    45181 aaaaaagaag ggtgactggt ttaatatcga tagtcaattg cctaatcggc tggcaattga
    45241 tggtatttta gacggcacga tccttttacc aggattaact ggtccattta cggtatacaa
    45301 gcgtgaagta acttatcttc actccaaatt tgatatttat ttagagacgg tggtcggaca
    45361 aaaagcattt gttgaagtca aaggtatgac acttgaaaat aaagcgattg gtgcttttcc
    45421 tgatgcgccc acactcagag gattgaaaca tataggagaa ttaacgtatg ccgcacaaga
    45481 tggttatgcc gcttatgtct tgtttattgc tcagttcgaa catcttcatc tggcaacgat
    45541 ccatgaagaa atgcaaccag cactagctga tatggttcga catgcgcagc aaagcggagt
    45601 tcaaatcctc gcctataatt gtcaagtatc ggcagatgag gtaactgtca agcaggccat
    45661 tccatttgat gtagatatcc cttttgaaga tcctatacaa tagaaagaag gtaaaataat
    45721 gatcaactta ggtattatcg gaacaaattg gattactcat caatttgtcc aagcagcttt
    45781 aagcacaaaa aaatatgaat tgacagcggt atattctaga catttggaaa aagcacagga
    45841 atttggttct cattatgaag gggatatcga attcgcaaca gatttgaaga cttttttcaa
    45901 tattactcat atggacacca tttatattgc ttctccgaat agtcttcact ttagtcaagc
    45961 taagcaagca attttggcag gaaaaaatgt gatagttgaa aagccagctt tttcgacgcc
    46021 aaaagaaatg gatgaaatca tccatttggc aaatgaacac aatgtgtatt tttttgaagc
    46081 agcaagaaat atccatgaat caggttttaa aaagattgcc gattacttac cattaaaaga
    46141 cgagataatc ggtgcaaatt tcagctatat gaaatattct tcgcgctatg accaagtatt
    46201 agatggagaa gaaccgaata tcttctcgac tcatttctca ggtggtgcat tgatggattt
    46261 aggtgtttac cttgtatatg cagctgttgc ttggtttgga atgccaaaag aagtccatta
    46321 tttcccaaga aagatttcta ctggtgtaga tggtcttgga tgggggatct tacgttatga
    46381 gaattttgat gtatcgatcc agccaggtaa aatcggtgat tcttatttac cgtcagagat
    46441 ttattttgac tcaggaacgc tagtgatgaa tggagtcaat gcaattgaac aagcacaatt
    46501 tcatgatcgc gcgcataaag aaatcattga tttggcggta acagctgcag aaaatccaat
    46561 ggcagatgaa gcagaggatt ttgccaatgt catggctcat ccgaaagatc cagcttgggg
    46621 attgttgtat gaagagtgga tcgagctagc tagaaatgtg aaccaagtga tttatgattt
    46681 aagaaaaaat ggcggcatca aattcgatgc agataatgag gaagattttt aatgaacgaa
    46741 tttgtacaag tatttcccga aaattggcag aaaaaatatg aggataaagg gtttatctca
    46801 ccttctctta ttcaacgagc agtttttcag ccactaagta ataaacaaag tatagtagcg
    46861 gtttctccta caggttcagg taaaacactg gcttatcttt ggcctctact actaaacgtg
    46921 gaaccaggtg aagcaagtgc gttggtcatt tttgcttctt ctcaagaact agctattcaa
    46981 gtagcagatg ttgcacgtga atggggaaaa gacaaagagt tgaaagtcca atctttggtg
    47041 ggcggagcca acgtcaaacg tcagattgag ggtttgaagg aaaaaccaga aatcttgatt
    47101 ggtacaccag gaagaatatt ggaattgatg aaagcaaaaa aaataaaagc acatcaagtg
    47161 aaaacaatgg tatttgatga agcagaccag ctatttgacg ctgggaacag ccaaatcatt
    47221 gatcaaattc ttcatcaagc tccaacagag tatcagctag cttttttttc agcgacagca
    47281 gatcgttccc ttgaaagcat tgaaaaaatc actgggaaaa cgtttgagac aattgacgtg
    47341 acagcagagg acgattctcg aaaaggactg agacattact ttttacgtgt accgatacga
    47401 aagaaagatg actacttgag acgtctcacg catatagaag attttcatgg tctgattttc
    47461 tttaatcaac tgactgaatt aggcgtaatg gaagaaaaat tgctgtatag aggtgtaccg
    47521 gtaggaagtc tggcctctga tcagaacaag ctactccgaa aagcagcgct agaacaattc
    47581 aaaaagaaaa tggtcagagc acttttgacc acagatgtgg cggctagagg tttagatatt
    47641 acaggtcttc cttatgtagt gaatgcagaa gttccgttaa gtgaagaagc ttatttgcat
    47701 cgttcgggaa gaactggtcg aatgggtaat gaaggaatag taattacact cgtacaagat
    47761 aataatctaa gagatttaaa acggctttta cgtccatcaa atataacact tgaagagatt
    47821 tttttatacg gaggtaagct gcaaactgag cagccaacca aagaagatgc agctcctgca
    47881 gtaaataaaa agtatgcgaa aactttttct ggaaaggagc agaacccaga atataacaaa
    47941 gaaaaaataa gaaaaaaagt taaaaataaa tcaaaaaaag agagaaataa aggagcaagg
    48001 cgcaaataag cgccttttta tgctatgatt atgaaaacgg gaacaaaatt ggttgccaga
    48061 ggaggggtta tgtgaaagaa cgtcaagaag agatcttgca gctattgaca gaaaaccaac
    48121 aaggattgac agcaagtgat gtagctgagc gcttaactat cgatcgaagc aatgcaagcc
    48181 gatatttaag tgaactatac aaggcccatc atattgtaaa aaccgctggc cgaccagtcg
    48241 tttactcttt gccaacggaa aagtcaaaat ctgatgaagt gcatgtcgac tcttctacgc
    48301 aagtgacttt tgaaacactc gttggagaaa atgattcttt aaaagtcagc atacagcagg
    48361 caaaagctgc gattctttat ccaccgcggg ggttgcatac gattattttt ggagaaactg
    48421 gtacagggaa gtcgatgttt gctgaatgta tgtatcattt tgcaatcgat tcagagatgc
    48481 tttctgcaga tgcgccattt gtttctttca attgtgcgga ttatgctcaa aatccgcaac
    48541 tgctctttgg acacatcttt gggatcaaaa aaggggctta cacaggagca gctcaagata
    48601 gtcccggtct aatagccaaa gcagacggcg gtatattgtt tttagatgag atccaccgtt
    48661 taccgccgga agggcaggaa atgctgttta ctttcattga taaaggtatt taccgtccgc
    48721 ttggagaaag tgcacaagta catgaagcat ctgttcagat cattggggcg acgactgaat
    48781 cttcagaaag tttcttatct acgttcaatc gacggattcc tatggcaatc accttgccga
    48841 atcttgcttc acgttcactg gatgagcgtt atgaaatcat ctctcttttt atcaaacaag
    48901 aagcgaatcg tttgaatcaa cgtatcgata tcgaaaaaga agcgattctt gccttcatgc
    48961 tttatgatgc ggaaggaaat atcggtcaag tgaaacgaga cttgaaacta gtctgtgcaa
    49021 aatctttttt acattaccgt actcatcatg agaaaaagct aatcattcga aaagaagaac
    49081 tttcccttca agtacaaaaa ggattactga aaatcaagga aataccggaa cgtctcgacc
    49141 gattcatgga cagcaaaagt cagtatttga catttgaacc aggatttgct gatgttgtct
    49201 ggtcacaaga tccagaacga aacatgcagg tctataacga tattgaggaa aaagtcactt
    49261 ctctttccga aaccggtgtg gagaacattg atctagagac cctcatttca aaagatgtgg
    49321 atgcttattt ccagacatat gtcaaagaat tgactaaaag ttcgattccc aaagatcttt
    49381 tgccagatga aatctggcgg ttaaccaatc aattgtatga tgtggctgaa aaacggttag
    49441 accgtattta taatgaaaaa gctcgttttg cttttgcttt gcatctgcaa agtacattgg
    49501 atcgtgtgag agaaggtcat atgatcgtcc acccagattt gaataacgta cggaaaaatt
    49561 ttaagaggga attccaggta gcaatcgatc tttcaacgat cattgaagaa gaagaacaag
    49621 ttgagattcc ttttgatgaa atcggcttta ttagtatgtt tctttctatc aatctaggag
    49681 aaagtgagcc tgttaaagaa aacaaagtag atgttctcat attgatgcac ggcagacaga
    49741 ctgcttcaag catgttggag atggcacaag aactattagg aacaaaagta gggaaagctt
    49801 ttaatatgcc actggttatg gaagttcaaa caatgtatga agaagtgtta caatatgtca
    49861 aaaactatca agagcatttg acgaatggcc tactcttgtt aacagatatg ggatcattaa
    49921 acaccttcag tgatttgatt tatgaagaga ctggcattcg gacgaaagct atcacgatga
    49981 caagtaccat gattgtgata gaagcgatac ggatggcaag tgttggaaga agcttagaag
    50041 atatttatca aaatatccag ctttcctttg aatcgatcgt acgtgatcag ttcaaagcgg
    50101 aaaagtctga aactttacag cgtaaaaaag ccgtaatcgt gacatgcttt actggtgaag
    50161 gagtagctgc caaattatat cagcgtatta tgccagtgat taatcaaaat aaggtagaac
    50221 tgatccagat gcagttttta gaacgtgaat cattcaaaaa acacatcgat gccttattgg
    50281 aagaatatga gatcaaagca attgcgggaa cagttgatgt tgattatcaa aatatcccat
    50341 ttttttcagc ctatgacgtt tttgatgatg aaaaactaaa tattttaaag cgtatagctg
    50401 atgacgagat cccgacagct aaaattgtcg attcacttgc tggatcatta agcgatgtct
    50461 tttctattga tgaactgatc cgttcattgc aaaagacagt tcagcagatc cagaccgatt
    50521 tgtcgattat cgtggaacct ggagtagata ctggaatcgt tatccattta gccttcatgt
    50581 tagacggatt gatcaaacaa gaaagccatc ggcaatttga gaaactgtct attttctcaa
    50641 aaatttatcg tttggaaatg gatatcgttc gggcaaactt gatggcacta gaacgaaaat
    50701 atcaggtaaa aattccagaa gatgatatcg catttcttac acaaatgttt cttgaaaata
    50761 aaataaaaaa atgaacacac taatcatact gtttacacac taaacatctg tgtgcgcact
    50821 ataaaaaaga gctaaaaagc atgaaaacgc tatttcagct ctttttttta ttggcatgct
    50881 tcttgcgtta tataaaagtg taaaaaaata aaaggagtgt taaaaatggc aaaaaaaaca
    50941 atcatgttag tttgttcagc agggatgagt acgagtttat tggtcacaaa aatgcaaaaa
    51001 gcagcagaag aaaaagggat ggaagccgat atttttgcgg tctctgcctc agatgcagat
    51061 aataatttag aatctaagaa tgtcgatgta ttattattag gtcctcaagt acgtttcatg
    51121 aaagctcaat ttgaacaaaa acttgcgcct aaaggtattc cattagatgt catcaatatg
    51181 caagactatg gcatgatgaa cggagaaaaa gttcttgcac aagcagaaaa tctaatgaaa
    51241 taaatcaagt atgcagttgg gggtataaca acgtggaaga tcaacaaaat ctagaagcca
    51301 tcatggggtt aatcatgttt ggcggtaatg caaagagcga tgcaatggaa gcgattgcgg
    51361 ctgctaaaat cggtgatttt gagttagcgg ataggaaaat cgctgatgca gaagaatcac
    51421 ttatacaagc gcatcatgca caaacagaca tgttgacaca agaagcacaa ggaaatcata
    51481 tgcaagtgac attattgact gttcacagtc aagatcactt aatgacatct attgcgttta
    51541 ctgatttagc aaaagaaatc atcgaacttt atcgtcgcgt atacagcgaa taaatacaac
    51601 ttcttttttg aacggaagaa gaaaaatttt tgaacggatt ttttattttg ttataaggag
    51661 aactctcttt atataaaaaa cttataggag gaagtaaaaa atgaacggac tatcaagttg
    51721 gatggagaaa tacatcctgc cggttgccgc aaaaatcgga gcgcaaaaac atttagttgc
    51781 tttgcgtgat gcttttatcg gcacactgcc cgcaacaatg gcgggatcag tcgctgtaat
    51841 gatcaatgca attattcgtg atttaccgca acagtttaac agtacctatg ctgacagttt
    51901 agcagcagac aaaggaatct ttgccgtatt gaatgtcatc atcggaatca atggttttgt
    51961 ctggaacggt acactagcaa tcgccggatt gatctttgcc ttttcttggg ggtataatct
    52021 tgcccgagct tatggagtaa atgatctagc tggggggatc gttggtttag ctaccttgat
    52081 tcaaggtatt tcctttggtc cagcattatc agcagcatta gatgtgaatg ttccttcaaa
    52141 tgtcgcagat gcaatcaata gttctgagat tggtgcaacg attgcagatg gcacactgtc
    52201 agttggatta tggggttggc taaaactaga tcatttgaat ggaaacgctt attttacagt
    52261 tatgatcatg ggagctattt cagtcattat tttttgtaaa ttgatgcttg ccaacattac
    52321 gatcaaaatg cctgattcag taccaccagc agtatctaaa gcatttgcag ccattttacc
    52381 agcgacgatc gctttatacg tcattgctat tatcaattat atcgtaggtc gtgtagctga
    52441 tgggcaactg atcattgatt tggtgcaaaa atatattgca gagccattcc taggattatc
    52501 tcaagggttg ggagcagtat tgatcgtcac agtatttgtt caagtattct ggttctttgg
    52561 tattcatgga ccaaatgtat tggcaccagt gctagaggga atctggggac aagcacaact
    52621 aatcaatatt gatattttcc aaaaaggtta taacggaaaa actggtacac cagccgtgtt
    52681 agatgcaatc gaagatggaa aagcttatat gtgggtccgc ggatcgttcg atgcttttgc
    52741 atggtttggc ggatcaggcg gaacgatcgt cttactgata gctatcttga ttttctctaa
    52801 acgtgcagat tatctaacag tagctaaatt atccattggg cctggtatct tcaatatcaa
    52861 cgaaccagtg atgtttggtc tgccaatcgt attgaatgca atcatgttta tcccattctt
    52921 gattgctcca gtagtagcaa cagcaattgg atggacagct acttatcttg gcctagtcgc
    52981 tccggtatcg cagcaagtca catgggtagt accgccgttc ttattgtcat tcttagcaac
    53041 aggagcggat tggcgggcac cgatcgtaac attagtatgt atggtcgtga ctttcttgat
    53101 ttgggcacca tttgtcatct ctgcaaacaa aatggaccct gccgcaggcg aacaacaata
    53161 agatgaacgt gaggagcggg acgctttaag tttcgttcct cataatgaat tttcgaggag
    53221 gaactgaaat gattacaatt gccagtgcaa tgcaacaaga tgaaatcaaa gaattgttag
    53281 aaggatacaa tgaagaagga atgactttta catttaaaga aaaacaagga atcaagctgt
    53341 tctttgaaac aagtgcagaa gatccggaag ttgcggctaa aaaagcaaag gaactaatca
    53401 aagcacagcc ttggggaaca gtcctttatt ttcaatcaac agctgtttag aaaatggttt
    53461 aaaaagatag gatggttgag atgataaaaa acggtttatt tttatgtagt attggttctt
    53521 tgatcgtgtt gtatagtttg aatccacatt cgcaaaggta tgatttgtat agtatggtca
    53581 caggtctttt tctaacagga ttaggaatct atttcttcat gaaggggaaa aaaagagaag
    53641 aagcaaagga gaaaaataac taatggaaat cacgaaaaaa ttaaatattc cagcatcgtt
    53701 tttttacgat aaagttatgg actctgtcct tttcgatgta cgtaaagcga ccggcaaatc
    53761 agtaactaga aaacagctga aaaatttgga gtacgtcaaa caattctcgc aaaataaccg
    53821 ggctcgcata aaaatcgaag aagtcattga aaaccagtct tataagtttc gtacatcgac
    53881 aacgaagaat gaattcgtcg ttcattatca aatcaaacca ttggacgatt cgacctgtga
    53941 ggttcattac acagaaaaaa tggagtctta tggcatgctt caaaagttga atgatatgct
    54001 tttaggaaca atcttgatgt attttaaacg gagacgtttc aaaaaaatgc tagatatgat
    54061 cgaagcaacg tattaaatca atgaaacatg gtactattta gttattgaaa aatgaagtaa
    54121 gctagaagga atttttcata ggaagataga tctttcctgc gaatcctcac tagctttttt
    54181 gtaggaaggg gcagcaaaat gacaaacaaa gtaagagagc taggtgtatc ttcgattgag
    54241 gaaatgtttg atttagccac ttatgcattt aatggggcag atacacctga aaggcgagaa
    54301 cgttttcgta cacttgcgga gaattcttgg aattacggat tttttgatga ggaggatcgg
    54361 ctaacaagtc aagtcatggc gacccctttt ccagtgaatt tttacggaca agagtaccta
    54421 atggccggca ttggctatgt tgcttcttat ccagaagcaa ggggacaagg cgggatcaat
    54481 cagatcatgg aaaagatcct tgaagattgc cgtgatcgta aagtaagctt atcttatctg
    54541 gcgccattct cctatccctt ctatcggcgc tatggctatg aacaatcatt tgataaaatc
    54601 agttatcaac taaacagtcg tgatgtgccg tttatcaaaa aaaaatctgg aaaaataaag
    54661 cgtatggatt ttgaagatgc aaaagctgca atcagaagaa tttatgaaca gatgccggaa
    54721 aaccaacgag ctggtctgaa gcgcgaagac tggtggtata cgtataaatt caagcaaaaa
    54781 ggcaattacc agtttgcttt gcattttgat gataatgagg aagcaacggg atatatcgtt
    54841 tatcagcttg caacgtcttc ctttattatt gcggagtggg gatttttgga tcatgatgct
    54901 ttttgcagcc ttgttcgctt cgtaagttct cacaatggtg cttttgaaac atttgaatat
    54961 tcatgcggct acggcggtaa tgatcaaaat tatctattag aaaatccatt tgcttctgta
    55021 atgatcacac cttatatgat ggcgaggatc gtagatattt ctttgttttt ggaaaattat
    55081 ccatttaaaa agagacaagc tgcatttgcc ctacaaatag aagaagatca ttatgcgaaa
    55141 tggaatgaag gaatcgtgga agtaacaatc gaaaatggaa aaaatcatct ctcaaaagta
    55201 gaacaaacaa aactcccaat cgttcgagga agtatccaac atatcacgca gctattatta
    55261 gggtatcgta aagtggaaga gttggtattc caagaaagaa tacaggtgac aaaagaatat
    55321 gaagaaacgt taagctcaat cttgccacag caacagccaa ttcttaatga ctatttttga
    55381 taaaaaacgg gtgatgacaa tatttttgtc accacccttt tgtttttcat ttggcatttg
    55441 aaggaaaatg ttaaagtaaa tcctcttttt tcgtaaattt gaattttttg ttttcttttg
    55501 ttttagggtt ttcttttttg tggtcttgga ttgcttcttt cgtaatcttt tcccgatttt
    55561 ttttagatga atctaagcgt tctgcgtata ctttttcttt tggcttatca ttcattggaa
    55621 aggacctccc tacattattt cattctacct ctagtttatc atctgttaag gactagtgga
    55681 aacgttttgc ctaacttata agctgatttt tttagcagat aagcttgcaa gtaaaaaaca
    55741 aacaagctac aattgattta ttgaaattgt tcctgtagct cagtaggata gagcaatcgc
    55801 ctcctaagcg ataggtcgct ggttcaattc cagtcaggaa cgtacaaata acccctgtat
    55861 cctttgcggt acagggggtt attttgtata agtagacatt gtagtttcta aactgctatg
    55921 tcctaaacgt ttcgatacgc taagtatatt tactccttga taaagtaaaa tagatgcatg
    55981 cgtatgtctt agtccatcaa taaacagtcg aagagaaaat agggaaaaat tgattactaa
    56041 agaaaaactt actaggtcga cggtgtagtt gattttcata cttcaataga ttcgaattaa
    56101 acgatttcga ttggcttata ccagtcctat ccaacgaaag atatatcgtt cttaactcta
    56161 gcggtataac ttgaagcttt acgtaaagcc aatgggaatg aactggagat gactttttat
    56221 tatttttagt aatagctgat gggttaatac ttacaacttt ccttttcaaa gtaccatata
    56281 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
    56341 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
    56401 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
    56461 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
    56521 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
    56581 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
    56641 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
    56701 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
    56761 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
    56821 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
    56881 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
    56941 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
    57001 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
    57061 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
    57121 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
    57181 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
    57241 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
    57301 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
    57361 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
    57421 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
    57481 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
    57541 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
    57601 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
    57661 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
    57721 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
    57781 aaaactaatt ttgcatatca aaaaaacatg ttacaatgaa attaatcaat ggtggaagcg
    57841 ttataaaaaa attaaggaga ttaaaaaata tggcaagaaa aaagacgatt actagagaac
    57901 agattttggc agctgcttat gaagtagctg ccaccgatgg atttacacga tttactgcga
    57961 gaaacatcgc gaacaaaatg aattgttcca cgcaaccgat ttatcttgag tttaaaaata
    58021 tggatgactt gagacaagct ctttttgagc agatctatga tcatttacaa tatgaagtgt
    58081 tcactgttga gcatacaggg aatacgatta tcgatcttgc ttttaattat atccattttg
    58141 caagaagaga gaagaagttg tatcgttcac tgtacttaga agaatatggt ggaggaaaag
    58201 agatgcagaa tttctcttat acttactttg tgaatacagt gaaacgcgat ccagcttatg
    58261 tgaatctttc agaagagcag atcgaatctt tgtataatgg gacatggatc atagctactg
    58321 ggatcgcagc tttgatgtct tcaagcatca tccatccaac tgatgaacag attgaaaaga
    58381 tgatccaaga cagtatagat gctatcttga aattcaaaga accaatcgaa atcgattgaa
    58441 tatactcaaa agttccacaa atcagtaggt aacaagagat gtgtcaaaat aaggaaaagt
    58501 tcgtttttct tacagcgtat gcatttttaa ctcccgaaga actcttccat gaatgcaatg
    58561 aataaaagag ccatacagat tgaataaatc tggatggctc ttttattgca agtatattaa
    58621 atttttgcga ctagttcttt agcgaaagct tctaaatttt cgatatcttc ttcttcagca
    58681 ttcagatcca ctttaacatt ttcggctcct tttgatgcgc ctgtttttgc aaaagcagca
    58741 tcgaaatcat ccaccgattt gcagaattca tcgtaaaaag tatcaccaga tccgcagaca
    58801 ccgtaaattt ttcccaatag atccaattct tgcaagtctt cataaaagtc tacgatctca
    58861 tcaggcagat cgccatcatc gtatgtataa gtagctacta cgcaaatatc tgcctcctcg
    58921 aattcatctg catctacttg ggtacattcg ttgatttcga cttcgatatc cagattttca
    58981 aatgcttctg caacaatatc ggcgatttct tcagtgtttc ctgtcatgct agcatatacg
    59041 atttttgcta aagccatgat gtgcctcctc gtttataatt atttaacaat tatgaattat
    59101 accaaaacat cattgaaaag aaaataaaac ctaaagacac ctggggaatg tcatttggct
    59161 aaatgtatgc cgctatgtta aaatgagagg gatttgtaaa aaggagacga aaatatgatt
    59221 actttaaaat cagcacgtga aattgaaatg atggcagaat ctggagcttt attagcagat
    59281 gtacataaaa atctgcgtga ttttataaaa ccaggaatca caagttggga tattgaggta
    59341 tttgtcagaa actatatcga gagtcatggc ggaattgctg cgcaaattgg ctttgaaggg
    59401 tatgaatatg cgacttgtat cagtataaat gatgaaattt gtcatggatt ccctcgtaaa
    59461 aagcctttga aaaacggtga tttggtaaaa gtcgatatgt gtatcgattt gaaaggcggc
    59521 atttctgatt cttgttgggc ctatgtagtt ggagaatcta cacctgagat cgaccgattg
    59581 atggaagtaa cgaagaaagc tttgtacatc ggtattgagc aagcacaagt agggaatcgg
    59641 attggtgaca tcggtcatgc tatccaaact tatgtagaag gcgaaaatct agcgatcgtt
    59701 cgtgatttta ttggtcatgg cgttggtcct acgattcatg aagaaccagc agttcctcat
    59761 tacggagaag ctggaaaagg gttgcggttg aaagaaggca tggttatcac gatcgaacct
    59821 atggttaata ccggaacttg gaaaatgaaa atggatccta acggctggac tgcgtataca
    59881 agagacggcg gactttcttg ccaatatgaa catacattgg ctattactaa agatggtcca
    59941 agaattttga catcacaagg agaagaaggc acttattgat ctatccagtc aataggtacg
    60001 ggagaggtac atgaaagttt ggaacaaaat aaaacaaaat gaaaatctga tgcgctttat
    60061 tgagacgact caaaagcgca tcactgattc agaaatgagt acgacctctg tcgtcgttgc
    60121 ttattattta ctcttgtctt tatttccatt gattattgct tttggtaata ttttaccgtt
    60181 tctccacatc gatcaagaga ctgtactgac ctatttacga gaagtgattc ctgagaccgt
    60241 ttaccagttt atcggtcctg caatcaaaga ccttttgaca caaagttcag gaggcttgtt
    60301 atccgtttct gcattagcgg ctttatggtc tgcaagtcaa agtatcaatg ctttgcaaat
    60361 tgcgatgaat aaagcgtatg gcgtagaaaa tagaaaaaat tttttgatcg tccgtttctt
    60421 ctcactaatt gtcattgtct tgttcatgat tgctattagt ggggtaacta tcgttcttgg
    60481 gttgggacag ctgattttgg atgctattca gccaatcgta caaatacccg tgaactttat
    60541 cgatcagttc caaactttga agtggccaat cacattggta gctttatttg tcatcatgtt
    60601 cttgatttat ttaattgtcc ctaatgctca gttgaagttg aagacgatca ttccgggcac
    60661 gatcttcgct actgtcggct ggatgctgtt atcacaagtg tttagtatct atgctaggta
    60721 ttttgcaacg aggatcagcg gttatcagat cataggaagt tttattgtac tcatgctttg
    60781 gcttaatttt gctgctacga tcatcatttt gggcggaatc atcaatgcag ttgttcaaga
    60841 atacgtgacg ggtcatcaaa tcaaaaaacg tcgagatcca agccgaaaat ggttcaataa
    60901 attgaaaact aaattttctc ataaaaaaaa gtagaaacaa gagttcttat tgtaaattta
    60961 tatcagtaat ttataataat tatgactctt aattaaagta ggtgcccata tgcttgtttc
    61021 ttttagtatg gttataagat tttttctgac aattttatta tcactgatat taacacctgt
    61081 attcaaaatc atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa gaagaataaa
    61141 taaagttcca atgccttcag cgggcggact gcctatcttt atttcatttg ttatatcgac
    61201 tttattctta ttccgtaaaa ttatccctca atcgtatatt gtaccgatct tgattgcttc
    61261 tttagtaatc atagtcacag gagtgctcga tgataaatat gagctgacac ctaaacaaaa
    61321 aagtataggg atcttgattg cttctcttat tatttatttt gtagcagata tacgtattga
    61381 ttccttcacg cttcctttta ttggatatat ccaactaggc tggcttagtt ttcccgtaac
    61441 gattttttgg atcttcggta tcacgaatgc ggtcaatctg attgacggtt tggatgggct
    61501 ggcggcaggg atttcattga tcggtttaat tacgatagga atcattggat attttttcct
    61561 ccatgcttct acagtgtata ttccagttgt tattttctgt cttgtagcaa gtattatcgg
    61621 tttttttcct tacaattttt atccggcaaa gatttattta ggcgatacag gagctctatt
    61681 cctagggttt atgatggccg tcctttcact acaagggctg aagaacgtga cttttgtttc
    61741 atcgatttct ttgctgatcg tcatgggagt gccggtaaca gacacgtttt ttgctatcat
    61801 taggagaaaa gcaaaccgtg tttctttttc tacagcagat aaaaaacatc ttcatcaccg
    61861 actaatcagt ttaggcttta ctcataaagg tgctgtcctg acgatttatg cgatggcttt
    61921 gatgttttct ttcacagcta tggtcatgaa ttatactgga agaatcggaa cgatcgtatt
    61981 gattattgcc atgttgtttg ctgcaatttt attatttgaa ttgattggtc tgatcaatga
    62041 gaaacatcag tttattttaa aaacattgcg atttttaggg aataaagagt accgtacgca
    62101 agtaaaacag aaatatttga agaaatggaa ccgtcatata gatgaataac aaattaaacc
    62161 agccgatcct tattagtatt gtgacggcaa acagtgataa aatttttcgg acactagata
    62221 attttatttc aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt gacaacaatt
    62281 cacttcccga gtacaaaaaa agactaacag agtataggaa atatccctac atcgatatct
    62341 attttcatga tgaaaattcc ggatttggtt atggacataa tcacaatctg atagctagcg
    62401 attctcgcta tggcattatt tgtaacccag atattttagt ggatgaagag acagttacag
    62461 cattggtctt gttattgaaa gaacatccgg aatgtgcgat gttagcacct aagattctta
    62521 atgaagatgg aacaactcag cacctcatcc gtgagcagct ggctgttttt gattacgttt
    62581 tgcgctttgt gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg gctgtgtttg
    62641 aatgccgagg attgcctact gatgctaata gttatgtacg gatgatctca ggtagtttta
    62701 tggtagctga tattgataaa tttaaagaga taaaggggtt tgatgagcgt ttcttcatgt
    62761 actttgaaga caacgacttg tgtttgagtt ttgaaaaagc aggttacaag ttattgtaca
    62821 caccctttta tgcagttatc catttttacg ggaagggtgc gcatcgaaat gcaaaactgt
    62881 ttcgcgtgtt tttgacttct atgatgaaat ttttcaataa gtgggggtgg cgttttttct
    62941 gatgaaacca gaaatattag tagtcgttgt gctttatcag caactattca gtcaatctcc
    63001 agcttatgga ccattaagca aagcacttag cgaaaagcaa attcagctta ttatttacga
    63061 taatagtcct caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg tttatcatca
    63121 tgatccgcaa aatcctggtt tagcggtggc ttataatttt gcgttgtctc aagcagatga
    63181 aggaacacgt tgtctcgtca ctctggatca ggatacgagg ctagtcgacg attattttga
    63241 actattgcgt aaagtaacct ttacagatga atgtgttgct gcagttccga tgattttttc
    63301 tgggagccga caagtttctc ccctatattc agatcgttat atcaatcgta cagctcaagc
    63361 agtagaagtt gaaggcccaa cagctgaacg aatcatggca atcaattcag gtgttgcctg
    63421 gtctgtcaaa tttctaaaaa agattggcgg gtttaatcct gctttttctt tagatttttt
    63481 ggatcattgg ctgttttgga aagtggatca gttggaaaaa actgtggaga tccttcctgt
    63541 acgaatggaa catgatcttt cggttttaga ctatgaaaaa gtttcaagta atcgataccg
    63601 ttctatttta caagcagaaa atcgatttta tcatgaatat gatcaaaggc atttatccag
    63661 tcaccgacga caattattgt taagaacagc taaacagttc ttgaccgtca aaaaccggca
    63721 tatctggcgg atgactttgc gctcatatgt taagaattgg aaggtgtgaa ttcgtgcgtt
    63781 cggtttgtat agctaccttt aatggaggca gatatttaag agagcagtta gatagtattt
    63841 tatctcagct ttcagcagaa gatgaagtag tcatttctga tgatgggtca gtagatgata
    63901 catgggatat attaactgaa tacgcagaaa aagataagcg aatccagctt ttttcggggc
    63961 ctcgtcaagg attgatcgct aattttggtt atgcgatcca gcagacgaaa ggagatctaa
    64021 tctttttggc agaccaagat gatgtttggt tggagacaaa agtgaaaaag atcactgact
    64081 attttgagca gaatccggat cagctagtag tagtttctga tttaatcatt gtggacagcg
    64141 agctgcaaga gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa ggatggctta
    64201 ataatgtgat aagaagtcac tatattggtg ctggtatggc tttccggtct tctttaaaac
    64261 ccgatatttt gccgatcccg tcagaagtgc cgatgcatga tatgtggatt ggtttgctgg
    64321 gaggaaagaa gactgggttc ctaagagaac cgttaactct gtaccgaaga catgatttaa
    64381 acgcgagtga gattgaaaca cattctaaat tacggcaaaa aatttcttgg cgtgttcatc
    64441 tgattacggc gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg tttaaatcct
    64501 taatatttcc atgacaaata taacaattga tgagataata acaaagtgaa atttgaaatc
    64561 aaaaaggaga gcagatatcc atgaagggaa tcattcttgc tgggggcagc ggaacacgtc
    64621 tgtacccttt aacaaaggca acatcgaaac aattgatgcc gatttatgac aaaccaatga
    64681 tttattatcc aatgtctact ctgatgttgg cgggaatcaa agaaattctg attatttcaa
    64741 cgccacaaga tacaccacgt ttcaaggaat tatttggaaa cggaaatgat ttaggactgc
    64801 agatcgaata tgcagtacaa gaaagtccag atggattggc acaagcgttt atcattggag
    64861 aagaatttat tggagatgat agtgtctgct tggtattagg ggacaatatt tactatggtg
    64921 gcggtctgtc taaaatgttg caacgagcag cttcaaaaga tacaggcgcg acagtatttg
    64981 gctatcatgt caatgatcca gaacgctttg gtgtagtaga atttgatgag aatatgcaag
    65041 ctttgtcgat tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta actggccttt
    65101 atttttatga caatgaggta atttctatcg ctaaaggtat caaaccttca gaacgtggag
    65161 aactagagat tacggatgtc aataaagcat atttggataa aggaaaacta tccgtagaac
    65221 ttatgggacg cggatttgct tggcttgata caggaacaca tgaatcattg cttgaagcat
    65281 ctacctttat cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt ttggaagaaa
    65341 ttgcttaccg tatgggctac atcgataagg aacaattgct ggcccttgca cagccattga
    65401 aaaaaaatca atacggtcaa tatttattga atttggccgc tgaataagga gtccttacag
    65461 atgaaagtaa cagatacaaa attaacagat gtcaaaatcg tagaaatgga cgtctttggc
    65521 gatcatcgcg gtttcttcac agaaagttat tcaaaagcaa aatttgcaga acatggattg
    65581 aaatatgact ttgttcaaga caaccattca ctatcagctg aagctggtgt ccttcggggg
    65641 cttcatttcc aaaaaggaga agctgcacag acaaaattga ttcgggtagt cactggagca
    65701 gttttagatg tagtagtgga tatccgcaaa ggtagcccga cttatggtca atgggacgga
    65761 tttatcttat ctgaacataa tcatcgacag ttgcttgtac ctaaaggata tgcccatgga
    65821 ttcgttactt tgacaccaaa tgtgaacttc ctttataagt gtgataatta ctataatgca
    65881 gaagcagatg ggggcatcgc atttgatgat ccagatttag cgattgattg gccaattgat
    65941 ccatcaagag cgattatgtc ggaaaaagat caccgtcatc cgacattaaa agaatttgaa
    66001 gcagaaaatc catttgttta cggagaaatc taagaattaa ggagaaattt tcttatgaaa
    66061 aacatcattg ttaccggagg tgccggattc atcggctcta atttcgttca ttacgtggta
    66121 aacaatcatc cagaagtgca tgtcactgtt ttggataaac tgacttatgc aggaaacaaa
    66181 gagaacttgg ccggacttcc tagcgatcgc gtagaattgg tcgtaggaga tgttgcagat
    66241 gcagaattag tggaccgtct agtaaaagaa gcggacgcag ttgttcatta cgcggcagaa
    66301 tcccataacg ataattcatt aaaagatcct ttcccatttg tacaaacgaa tatcattggt
    66361 acgtatacac ttttagaagc ttgccgcaag tatgatgtac gctatcatca cgtatctact
    66421 gacgaggttt acggcgatct tccattacgt gaagacctgc caggacatgg ggaaggcgaa
    66481 ggagaaaaat tcactgcgga aacaccatat aatccttcaa gtccatattc ttctacaaaa
    66541 gctggttcag atctgttagt aaaagcatgg gtgcgttctt ttggcttacg tgcaaccatt
    66601 tcaaactgtt caaacaacta tggtccttat cagcatattg aaaaatttat tcctcgccaa
    66661 gtaacgagta tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa gaatgttcgt
    66721 gactggatcc atacgaatga tcattcttca gcagtgtggc tgattttgac gaaaggcaga
    66781 attggcgaaa cctatctgat cggtgcagac ggtgaagaag ataataagac cgtgatggaa
    66841 ttgatcttag aaatgatggg ccaaccaaaa gacgcatacg accatgtaaa cgaccgcgca
    66901 gggcacgatt tgcgttatgc aatcgattcg acaaaattgc gagaagagct aggttggcag
    66961 cctgagttta cgaacttccg tgatgggttg gctgacacga tcaaatggta cgaagaacat
    67021 gaagactggt ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa aaatggacaa
    67081 taatacagag gaaatttcac tgtattaatg gaaaaacaca tgtgaggcag gcgctcgcct
    67141 gtctcttttt taaaataaaa atcaaggagc ggataaccat atgtttttat taactggagg
    67201 aaatggacaa ttaggtacag aattgcgcca tttattagat gaaaaaaatc tgccatatgt
    67261 ttcaacagat gcaaaagact tggatatcac agatgctgag aaaacaatgg cttatattac
    67321 agaattaaaa cccgatgtta ttttccattg cgcagcttac acagcagtag ataaagcaga
    67381 agaagaagca aaagaattag atgagaaaat caatgtcgat ggcacaagaa atgtagcact
    67441 tgcagcaaaa gctgtgaatg cacaattcgt ctatatcagt acagactatg ttttcgatgg
    67501 aaaaaacaaa aatgtagaat acgaagtaga cgaccaaacg aatccactaa atgaatatgg
    67561 tcgtacgaaa ttattaggcg aaaaagcagt agaagagatt ttagaagact attatatcat
    67621 acgaacttct tgggttttcg ggatctatgg acacaatttc gtcttcacga tgcaacgcct
    67681 agcggaaacc cgcgacaaac tgactgtagt aaatgaccag tttgggcgac caacttggac
    67741 acgtactttg gcagaattta tggtatatgt catcgaagaa aaagcaccat ttggtattta
    67801 tcatttgtcg aacgaaaata gctgttcttg gtatgagttc gcaaaagaaa tattgaaaga
    67861 tacagatgta gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag caacacgtcc
    67921 gcagtattca gtcatgagcc tgaaaaagac agaagcgtta ggttttgtca taccaacttg
    67981 gcaagaagca cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt aatttccttt
    68041 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    68101 aaaagccaaa agcgaatcag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    68161 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    68221 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    68281 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    68341 tctcacaagc tgaagaggac ttttcgttgg tgaacgagga ttattcatta aacggacaaa
    68401 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    68461 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    68521 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag aggaaaaaag
    68581 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    68641 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgattgct ttttatcatt
    68701 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    68761 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    68821 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    68881 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    68941 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    69001 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    69061 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    69121 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    69181 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    69241 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    69301 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    69361 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    69421 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    69481 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    69541 tttacctcgc ttttgtcgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    69601 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    69661 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    69721 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    69781 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    69841 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    69901 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    69961 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    70021 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    70081 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatatt agtagaaagg
    70141 agagtgaaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    70201 ttgcttttgt tgctgacgct agtctttttt gtttggacag caggggtagg ttttccagca
    70261 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    70321 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    70381 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    70441 ctgtttcctt tggttatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    70501 gatcgcggta tttatctgat tgtctttacg gtcttcctcg gaatcacgtt atatcgtgta
    70561 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    70621 gatatttgga tcaatacgaa tacaatcgga agttctttga tgactcttgc tttattgatc
    70681 agtggatttg ccagttcttt tgaacgatgg tatgttcgaa tcatcggtgg gatagcagtc
    70741 gttgcagcct ttttaggtat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    70801 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    70861 attgcctaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    70921 gaaaaagtca atttgtttac cgatcgagaa gatatttggc ataaattcta tcagacgcta
    70981 ggagaaaaat ctgaacaagt tttactagga atgaaaacat ttatcttcca gcgaggaaac
    71041 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    71101 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    71161 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    71221 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    71281 gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca
    71341 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    71401 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    71461 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    71521 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    71581 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    71641 aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt
    71701 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    71761 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    71821 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    71881 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    71941 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    72001 acacccattt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    72061 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    72121 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    72181 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    72241 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    72301 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    72361 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    72421 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    72481 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    72541 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    72601 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    72661 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    72721 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat
    72781 ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga
    72841 tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag
    72901 cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat
    72961 tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt
    73021 tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat
    73081 tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga
    73141 tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt
    73201 ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa
    73261 agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact
    73321 atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat
    73381 tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat
    73441 caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt
    73501 gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt
    73561 ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat
    73621 cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa
    73681 tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta
    73741 aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac
    73801 aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg
    73861 ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac
    73921 agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa
    73981 cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta
    74041 acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt
    74101 atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt
    74161 ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct gtaggtgata
    74221 cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc
    74281 gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa
    74341 gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa
    74401 cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga
    74461 cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac
    74521 ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga
    74581 atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt
    74641 tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg
    74701 gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca
    74761 caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg
    74821 aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg
    74881 aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa
    74941 aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa
    75001 aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata
    75061 agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca
    75121 atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg
    75181 agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg
    75241 aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga
    75301 aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt
    75361 gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa
    75421 tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga
    75481 tgttaagacc caaagcggtt tcaaggtacg tttatcaggg aaaatgagag gaaaagccat
    75541 cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata
    75601 cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg
    75661 gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt
    75721 gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa
    75781 agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc
    75841 tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac
    75901 gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa
    75961 gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa
    76021 tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc
    76081 actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct
    76141 aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg
    76201 caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa
    76261 ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg
    76321 taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc
    76381 tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc
    76441 aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac
    76501 agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt
    76561 ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa
    76621 gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc
    76681 aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt
    76741 tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt
    76801 caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt
    76861 cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc
    76921 acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca
    76981 gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga
    77041 tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac
    77101 tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac
    77161 attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta
    77221 caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga
    77281 agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg
    77341 gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag acggaatcgg
    77401 cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc
    77461 gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa
    77521 aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa
    77581 attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca
    77641 ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt
    77701 attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct
    77761 gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat
    77821 tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt gatggagata
    77881 accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat
    77941 atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct
    78001 tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt
    78061 tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc
    78121 gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg
    78181 gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa
    78241 ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt
    78301 gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag
    78361 ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa
    78421 tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt
    78481 gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac
    78541 tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca
    78601 atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct
    78661 tttttataac cattccattc atctgtataa acttctaccc catccttata tgcgacaatt
    78721 tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga
    78781 tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga
    78841 ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc
    78901 tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc
    78961 tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt
    79021 gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg
    79081 cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat
    79141 taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat
    79201 attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg
    79261 tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt
    79321 gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag
    79381 tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt
    79441 gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct
    79501 cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag
    79561 tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa
    79621 ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta
    79681 tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga
    79741 agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt
    79801 gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga
    79861 aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt
    79921 gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac
    79981 agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac
    80041 aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc
    80101 aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt
    80161 tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat
    80221 gtcgatcgta ggaccacgtc ctgaacgttc tttcttcgtc gatcagttca atgcagaaaa
    80281 cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta
    80341 tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa
    80401 atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa
    80461 aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt
    80521 tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag
    80581 cataatcgta ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa
    80641 agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac
    80701 acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac
    80761 caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct
    80821 cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt
    80881 gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga
    80941 cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca
    81001 cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg
    81061 gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa
    81121 tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat
    81181 gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata
    81241 ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga
    81301 cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt
    81361 ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat
    81421 attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa
    81481 aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt
    81541 tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta
    81601 gttaatggag gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg
    81661 tggagcagag tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt
    81721 tgattttttg atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag
    81781 attaggaggc cgtatttttg ttgtacctaa tcctaagaaa aatattttta aatatttaat
    81841 agaagtgaat aagttactaa aaaaagaatc tttttctata gttcacaatc aggtttattt
    81901 tggcggggga attaatttat ggttagccaa aaagaatgga atcagacaga gaattgctca
    81961 tagccatgca actgaagatg gaaaatctca aaatattgta atgaatgtat tgcgtaaatt
    82021 tttaaccaaa ttgttgttac aaaatgcaac agactacctt gccgttagtc aagaagctgg
    82081 tgaaagtctg tttcaaaacc atccctttga gatagttcat aatggtattg atttagagct
    82141 ttatagtaaa aattcagaag ctaaggttaa taagagaaaa gaattggata tctcaatgag
    82201 tacttttgtt gtgggaaaca ttggacgatt agagaaacaa aaaaatcaat cttatttgtt
    82261 agagattttt ttagaaatac taaaaatgaa ttctgattct tacttgattt tagtaggtga
    82321 agggtcactt agaaataaac tgaaagccaa agcagtagaa ttggatatag agaaaagagt
    82381 aaaattttta ggagaaagaa acgatattcc cgagttatta tctgtgatgg atgtttttgt
    82441 aatgccttct ctttatgaag gtctgccaat ggttggatta gaagcacaag cagcaaaatt
    82501 aaaattagtc ttagcagata ctatttccaa agatacaaag ttaacaaata atgtggattt
    82561 tgtatcatta gatgaaacac cacaagaatg ggcaaaaaaa gtaataaaag agccgtatgc
    82621 taataaaata acacaagaac taaagagtta tgatgtttcg tacactactg aacaaatgga
    82681 gagtatatat agtagagatc attaaaaatg ttcaagatat tttaggagtg attatttttg
    82741 aaaatatgtt atttgatatt ggcatacaat gatattggca acttaaagag attggtcaat
    82801 cgattgaacg aaaatgcagt ttttattatt catatagata aaaaaactga tatagaacca
    82861 tttattgaag catttaaatc atatgataat gttgaatttt tatgtaataa aaaccgtgtt
    82921 aaaatttatt ggggtggttt ttccattgta caagcagaaa tcaatttagt aaagagagca
    82981 ttaca
//
LOCUS       DAJSAW010000004        76631 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJSAW010000004 DAJSAW010000000
VERSION     DAJSAW010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.004"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..831
                     /locus_tag="ONT64_000315"
     CDS             <1..831
                     /locus_tag="ONT64_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR2301757.1"
                     /translation="ALLEISDSDPLLRYYVDSTTHEIILSFLGKVQMEVISALLQEKY
                     HVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVS
                     LGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLTPIV
                     LEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEI
                     PARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT
                     "
     gene            929..1117
                     /locus_tag="ONT64_000316"
     CDS             929..1117
                     /locus_tag="ONT64_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2301758.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1175..1528)
                     /locus_tag="ONT64_000317"
     CDS             complement(1175..1528)
                     /locus_tag="ONT64_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2301759.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2059..2481
                     /locus_tag="ONT64_000318"
     CDS             2059..2481
                     /locus_tag="ONT64_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2301760.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2478..2708
                     /locus_tag="ONT64_000319"
     CDS             2478..2708
                     /locus_tag="ONT64_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2301761.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3206..3406
                     /locus_tag="ONT64_000320"
     CDS             3206..3406
                     /locus_tag="ONT64_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCR2301762.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3433..4626
                     /locus_tag="ONT64_000321"
     CDS             3433..4626
                     /locus_tag="ONT64_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2301763.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4694..6259)
                     /gene="guaA"
                     /locus_tag="ONT64_000322"
     CDS             complement(4694..6259)
                     /gene="guaA"
                     /locus_tag="ONT64_000322"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR2301764.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(6318..7241)
                     /locus_tag="ONT64_000323"
     CDS             complement(6318..7241)
                     /locus_tag="ONT64_000323"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR2301765.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7437..8042
                     /locus_tag="ONT64_000324"
     CDS             7437..8042
                     /locus_tag="ONT64_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR2301766.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            8350..9366
                     /locus_tag="ONT64_000325"
     CDS             8350..9366
                     /locus_tag="ONT64_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR2301767.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9507..10808
                     /locus_tag="ONT64_000326"
     CDS             9507..10808
                     /locus_tag="ONT64_000326"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCR2301768.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10854..11516
                     /gene="deoC"
                     /locus_tag="ONT64_000327"
     CDS             10854..11516
                     /gene="deoC"
                     /locus_tag="ONT64_000327"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR2301769.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11537..11935
                     /locus_tag="ONT64_000328"
     CDS             11537..11935
                     /locus_tag="ONT64_000328"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR2301770.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            12001..13083
                     /locus_tag="ONT64_000329"
     CDS             12001..13083
                     /locus_tag="ONT64_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2301771.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            13295..14857
                     /locus_tag="ONT64_000330"
     CDS             13295..14857
                     /locus_tag="ONT64_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2301772.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14850..15983
                     /locus_tag="ONT64_000331"
     CDS             14850..15983
                     /locus_tag="ONT64_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2301773.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15983..16939
                     /locus_tag="ONT64_000332"
     CDS             15983..16939
                     /locus_tag="ONT64_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2301774.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            17038..18204
                     /gene="deoB"
                     /locus_tag="ONT64_000333"
     CDS             17038..18204
                     /gene="deoB"
                     /locus_tag="ONT64_000333"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR2301775.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            18228..19046
                     /locus_tag="ONT64_000334"
     CDS             18228..19046
                     /locus_tag="ONT64_000334"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR2301776.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            19063..19770
                     /gene="deoD"
                     /locus_tag="ONT64_000335"
     CDS             19063..19770
                     /gene="deoD"
                     /locus_tag="ONT64_000335"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR2301777.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(20068..20754)
                     /gene="gpmA"
                     /locus_tag="ONT64_000336"
     CDS             complement(20068..20754)
                     /gene="gpmA"
                     /locus_tag="ONT64_000336"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR2301778.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20926..21507)
                     /locus_tag="ONT64_000337"
     CDS             complement(20926..21507)
                     /locus_tag="ONT64_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2301779.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(21640..22323)
                     /gene="rpiA"
                     /locus_tag="ONT64_000338"
     CDS             complement(21640..22323)
                     /gene="rpiA"
                     /locus_tag="ONT64_000338"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR2301780.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            22642..23055
                     /gene="rpsL"
                     /locus_tag="ONT64_000339"
     CDS             22642..23055
                     /gene="rpsL"
                     /locus_tag="ONT64_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR2301781.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            23139..23609
                     /gene="rpsG"
                     /locus_tag="ONT64_000340"
     CDS             23139..23609
                     /gene="rpsG"
                     /locus_tag="ONT64_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR2301782.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            23703..25790
                     /gene="fusA"
                     /locus_tag="ONT64_000341"
     CDS             23703..25790
                     /gene="fusA"
                     /locus_tag="ONT64_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR2301783.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            25962..27149
                     /gene="tuf"
                     /locus_tag="ONT64_000342"
     CDS             25962..27149
                     /gene="tuf"
                     /locus_tag="ONT64_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR2301784.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            27556..27864
                     /gene="rpsJ"
                     /locus_tag="ONT64_000343"
     CDS             27556..27864
                     /gene="rpsJ"
                     /locus_tag="ONT64_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR2301785.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            27895..28524
                     /gene="rplC"
                     /locus_tag="ONT64_000344"
     CDS             27895..28524
                     /gene="rplC"
                     /locus_tag="ONT64_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR2301786.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            28552..29175
                     /gene="rplD"
                     /locus_tag="ONT64_000345"
     CDS             28552..29175
                     /gene="rplD"
                     /locus_tag="ONT64_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR2301787.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            29175..29465
                     /gene="rplW"
                     /locus_tag="ONT64_000346"
     CDS             29175..29465
                     /gene="rplW"
                     /locus_tag="ONT64_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR2301788.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            29504..30337
                     /gene="rplB"
                     /locus_tag="ONT64_000347"
     CDS             29504..30337
                     /gene="rplB"
                     /locus_tag="ONT64_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR2301789.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            30380..30658
                     /gene="rpsS"
                     /locus_tag="ONT64_000348"
     CDS             30380..30658
                     /gene="rpsS"
                     /locus_tag="ONT64_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR2301790.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            30681..31028
                     /gene="rplV"
                     /locus_tag="ONT64_000349"
     CDS             30681..31028
                     /gene="rplV"
                     /locus_tag="ONT64_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR2301791.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            31042..31698
                     /gene="rpsC"
                     /locus_tag="ONT64_000350"
     CDS             31042..31698
                     /gene="rpsC"
                     /locus_tag="ONT64_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR2301792.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            31701..32135
                     /gene="rplP"
                     /locus_tag="ONT64_000351"
     CDS             31701..32135
                     /gene="rplP"
                     /locus_tag="ONT64_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR2301793.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            32125..32313
                     /gene="rpmC"
                     /locus_tag="ONT64_000352"
     CDS             32125..32313
                     /gene="rpmC"
                     /locus_tag="ONT64_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR2301794.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            32338..32604
                     /gene="rpsQ"
                     /locus_tag="ONT64_000353"
     CDS             32338..32604
                     /gene="rpsQ"
                     /locus_tag="ONT64_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR2301795.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            32662..33030
                     /gene="rplN"
                     /locus_tag="ONT64_000354"
     CDS             32662..33030
                     /gene="rplN"
                     /locus_tag="ONT64_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR2301796.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            33067..33375
                     /gene="rplX"
                     /locus_tag="ONT64_000355"
     CDS             33067..33375
                     /gene="rplX"
                     /locus_tag="ONT64_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR2301797.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            33403..33942
                     /gene="rplE"
                     /locus_tag="ONT64_000356"
     CDS             33403..33942
                     /gene="rplE"
                     /locus_tag="ONT64_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR2301798.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            33961..34146
                     /locus_tag="ONT64_000357"
     CDS             33961..34146
                     /locus_tag="ONT64_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCR2301799.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            34182..34580
                     /gene="rpsH"
                     /locus_tag="ONT64_000358"
     CDS             34182..34580
                     /gene="rpsH"
                     /locus_tag="ONT64_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR2301800.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            34612..35148
                     /gene="rplF"
                     /locus_tag="ONT64_000359"
     CDS             34612..35148
                     /gene="rplF"
                     /locus_tag="ONT64_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR2301801.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            35309..35665
                     /gene="rplR"
                     /locus_tag="ONT64_000360"
     CDS             35309..35665
                     /gene="rplR"
                     /locus_tag="ONT64_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR2301802.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            35686..36186
                     /gene="rpsE"
                     /locus_tag="ONT64_000361"
     CDS             35686..36186
                     /gene="rpsE"
                     /locus_tag="ONT64_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR2301803.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            36201..36380
                     /gene="rpmD"
                     /locus_tag="ONT64_000362"
     CDS             36201..36380
                     /gene="rpmD"
                     /locus_tag="ONT64_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR2301804.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            36424..36864
                     /gene="rplO"
                     /locus_tag="ONT64_000363"
     CDS             36424..36864
                     /gene="rplO"
                     /locus_tag="ONT64_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR2301805.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            36864..38159
                     /gene="secY"
                     /locus_tag="ONT64_000364"
     CDS             36864..38159
                     /gene="secY"
                     /locus_tag="ONT64_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR2301806.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            38221..38868
                     /locus_tag="ONT64_000365"
     CDS             38221..38868
                     /locus_tag="ONT64_000365"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR2301807.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            39059..39277
                     /gene="infA"
                     /locus_tag="ONT64_000366"
     CDS             39059..39277
                     /gene="infA"
                     /locus_tag="ONT64_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR2301808.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            39310..39426
                     /gene="rpmJ"
                     /locus_tag="ONT64_000367"
     CDS             39310..39426
                     /gene="rpmJ"
                     /locus_tag="ONT64_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR2301809.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            39444..39809
                     /gene="rpsM"
                     /locus_tag="ONT64_000368"
     CDS             39444..39809
                     /gene="rpsM"
                     /locus_tag="ONT64_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR2301810.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            39837..40226
                     /gene="rpsK"
                     /locus_tag="ONT64_000369"
     CDS             39837..40226
                     /gene="rpsK"
                     /locus_tag="ONT64_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR2301811.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            40306..41244
                     /locus_tag="ONT64_000370"
     CDS             40306..41244
                     /locus_tag="ONT64_000370"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR2301812.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            41272..41652
                     /gene="rplQ"
                     /locus_tag="ONT64_000371"
     CDS             41272..41652
                     /gene="rplQ"
                     /locus_tag="ONT64_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR2301813.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            41924..42763
                     /locus_tag="ONT64_000372"
     CDS             41924..42763
                     /locus_tag="ONT64_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR2301814.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            42739..43608
                     /locus_tag="ONT64_000373"
     CDS             42739..43608
                     /locus_tag="ONT64_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR2301815.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            43598..44395
                     /locus_tag="ONT64_000374"
     CDS             43598..44395
                     /locus_tag="ONT64_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR2301816.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            44598..45344
                     /gene="truA"
                     /locus_tag="ONT64_000375"
     CDS             44598..45344
                     /gene="truA"
                     /locus_tag="ONT64_000375"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR2301817.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(45677..48346)
                     /locus_tag="ONT64_000376"
     CDS             complement(45677..48346)
                     /locus_tag="ONT64_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HCR2301818.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(48584..48772)
                     /locus_tag="ONT64_000377"
     CDS             complement(48584..48772)
                     /locus_tag="ONT64_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR2301819.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            49042..49404
                     /locus_tag="ONT64_000378"
     CDS             49042..49404
                     /locus_tag="ONT64_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR2301820.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            49456..51138
                     /locus_tag="ONT64_000379"
     CDS             49456..51138
                     /locus_tag="ONT64_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HCR2301821.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            51255..53291
                     /gene="recG"
                     /locus_tag="ONT64_000380"
     CDS             51255..53291
                     /gene="recG"
                     /locus_tag="ONT64_000380"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR2301822.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            53340..54341
                     /gene="plsX"
                     /locus_tag="ONT64_000381"
     CDS             53340..54341
                     /gene="plsX"
                     /locus_tag="ONT64_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR2301823.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            54463..54705
                     /gene="acpP"
                     /locus_tag="ONT64_000382"
     CDS             54463..54705
                     /gene="acpP"
                     /locus_tag="ONT64_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR2301824.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            54973..55977
                     /locus_tag="ONT64_000383"
     CDS             54973..55977
                     /locus_tag="ONT64_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2301825.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            55978..56922
                     /locus_tag="ONT64_000384"
     CDS             55978..56922
                     /locus_tag="ONT64_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2301826.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            56922..57884
                     /locus_tag="ONT64_000385"
     CDS             56922..57884
                     /locus_tag="ONT64_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2301827.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            57911..58825
                     /locus_tag="ONT64_000386"
     CDS             57911..58825
                     /locus_tag="ONT64_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2301828.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            58851..60632
                     /locus_tag="ONT64_000387"
     CDS             58851..60632
                     /locus_tag="ONT64_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2301829.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            60980..61666
                     /locus_tag="ONT64_000388"
     CDS             60980..61666
                     /locus_tag="ONT64_000388"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR2301830.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            61686..65267
                     /gene="smc"
                     /locus_tag="ONT64_000389"
     CDS             61686..65267
                     /gene="smc"
                     /locus_tag="ONT64_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCR2301831.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            65276..66085
                     /locus_tag="ONT64_000390"
     CDS             65276..66085
                     /locus_tag="ONT64_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2301832.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            66096..67094
                     /gene="ftsY"
                     /locus_tag="ONT64_000391"
     CDS             66096..67094
                     /gene="ftsY"
                     /locus_tag="ONT64_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR2301833.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            67281..69548
                     /gene="gshAB"
                     /locus_tag="ONT64_000392"
     CDS             67281..69548
                     /gene="gshAB"
                     /locus_tag="ONT64_000392"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HCR2301834.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(69637..70089)
                     /locus_tag="ONT64_000393"
     CDS             complement(69637..70089)
                     /locus_tag="ONT64_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HCR2301835.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            70239..71189
                     /locus_tag="ONT64_000394"
     CDS             70239..71189
                     /locus_tag="ONT64_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCR2301836.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            71182..72141
                     /locus_tag="ONT64_000395"
     CDS             71182..72141
                     /locus_tag="ONT64_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCR2301837.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            72138..72893
                     /locus_tag="ONT64_000396"
     CDS             72138..72893
                     /locus_tag="ONT64_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2301838.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            72916..73869
                     /locus_tag="ONT64_000397"
     CDS             72916..73869
                     /locus_tag="ONT64_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2301839.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(74513..74809)
                     /locus_tag="ONT64_000398"
     CDS             complement(74513..74809)
                     /locus_tag="ONT64_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HCR2301840.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            75164..75520
                     /locus_tag="ONT64_000399"
     CDS             75164..75520
                     /locus_tag="ONT64_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR2301841.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            75530..76429
                     /locus_tag="ONT64_000400"
     CDS             75530..76429
                     /locus_tag="ONT64_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCR2301842.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        24959 a        13851 c        16436 g        21385 t
ORIGIN      
        1 gcccttttgg aaatctcaga tagtgatccg cttctacgat attacgtgga ttctacgaca
       61 catgaaatta tactttcttt cttagggaaa gtacaaatgg aagtgattag tgcactgttg
      121 caagaaaagt atcatgtgga gatagaacta aaagagccta cagtcattta tatggagaga
      181 ccgttaaaaa atgcagaata taccattcac atcgaagtgc cgccaaatcc tttctgggct
      241 tccattggtt tatctgtatc accgcttccg ttgggaagtg gaatgcagta tgagagctcg
      301 gtttctcttg gatacttaaa tcaatcattt caaaatgcag ttatggaagg gatacgctat
      361 ggttgcgaac aaggattata tggttggaat gtgacggatt gtaaaatctg ttttaagtac
      421 ggtttatact atagccctgt tagtactcca gcagattttc ggatgcttac tcctattgta
      481 ctggagcaag cctttagaaa agctggaaca gaattgttag agccatatct tagttttaaa
      541 gtttatgcac cacaggaata tctttcacgg gcatataacg atgctcccaa atattgtgca
      601 aatatcgtaa atactcaact gaaaaataat gaggtcatta ttattggaga aattcctgct
      661 cgatgtattc aagattatcg caatgattta acttttttta caaatgggct tagtgtttgt
      721 ttagcagagc taaaaggata tcaggttacc actggcgaac ctgtttgcca gacccgtcgt
      781 ctaaatagtc ggatagataa agtaagatat atgttcaata aaataactta gtgcgtttta
      841 tgttgttata taaatatggt ttcttattaa ataagatgaa atattcttta atatagattt
      901 gaattaaagt ggaaaggagg agattgttat tataaactac aagtggatat tgtgtcctat
      961 ttgtggaaat aaaacaagac tacgaatacg agtggatact atacttaaaa atttcccttt
     1021 atactgcccc aaatgtaaga acgaaacttt aattaatgtt caaaaaatga atataataac
     1081 aatcaaagag ccagacgcca agacgcagag ccgataattt gagaaatgaa actctcatct
     1141 tatcggctct ttttgtttat ctgaatttta ctgactagcc ttcaatattt cttgcttcat
     1201 tgataccttt tgctagtgct tccattaagg atagttcttt gtctgtaaag ctatccatgt
     1261 atttttctac ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt
     1321 catcaataga tacatggagt aatgatacaa gatcatacag cacttgtgta cttgggtgtt
     1381 gtcctttgtt ttctatattt gttaaataac gtgggtcaat ttcaatcatt gttcccactt
     1441 gttcacgagt gagaccccgt ttcattcgag cctctttaat cgctaaacca acggctctaa
     1501 aatcgtattt atcttctttt ttacgcatac taaatcacct ctatacattt tactgttcct
     1561 atataaccat aaacaggtat agaaaaacat gttatatagt ttatgagttc ctatagaaca
     1621 ctgtggaatt tattaatgcg atgaatccaa tttcaaatag aaaggtcgct gattttattt
     1681 acctatacaa ctttataaaa tttagtcata ctcctagaaa gtagataaaa ttggcgactt
     1741 aatggaggga aaagtcgcca tgagaaagat aatctgtcat ctagttctct taattaagtt
     1801 cttatcaaaa aaacttatcc acctgtactg gatagatatg accatcaatg tggttttgca
     1861 gattacatag atagaaaaat caaagcatat gaacagagaa aacaatctgt ttatgtgctt
     1921 ttttggtcac ttaataaata tcttttagga tttctcttca tttatgcaac aaatcccccg
     1981 ttcgtgtcgg ggtttagtat gaagggagat aaagaagcac atgccactcc ctttcacgac
     2041 agaaaggggg tgaaaagcat gaaaccatct tcttttcaag aaaccataga aaatcagttt
     2101 gactatatct gcaaacaagt tattgaagat gaacgaaaag attacttcaa acatctagac
     2161 agactatcag caaaagaagt ctcgttttct gatatagata actaccttgt caactctttt
     2221 tctactgttg atacgtacgt cactgatttc caactattta aattatttgg attaagaatc
     2281 aatgttgaaa gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa aaaacggaac
     2341 attattttac ttcattactt tatggatatg agtgatgtgg agattgcgga gttactaaat
     2401 ttgaatcgtt cgactgttta tcgtcatcga ataagtggac tagccatgat taaagaattc
     2461 atgaaggagt gtaaagaatg aaaacagctt atccaagggt tcccttcccc ctcattgtaa
     2521 aggcgacaga tggcgacgtg gaagcaatta atcagattgt taaacattat cgagggtata
     2581 cctcaaaacg ttcattacgt cgcatgacag acgaatatgg aaattctcat atggttattg
     2641 atgaaaccct acgtggcaga atggaaacga aactcatctc taagatttta tcgttcgaaa
     2701 ttaaatagtc ccattttttc gtggaagcgt gtcgaatcgt atccacgctt cccgatttgg
     2761 gaggtttgtt attccatcaa agcatattgt gtgttcagta ggttttgata ggctaacaag
     2821 ccaaacgttc tttgacaacc aaataaaggc aatcgaatac gtttcggtaa gaaaagagct
     2881 gatgaactaa cacgccatga cctacaaaat tagtgagcga ttcatgttag taattcgaaa
     2941 gtaatcttta gcaggattgc tgccatgact ttcttatcgt gataatgata ctcccgtata
     3001 gccatagtcc gagcgttaga agcgtcacag gcaatgggta cggctacatg agaaccatgc
     3061 agaagtgaaa ttcctgtgag ctttgctaga gctgttcgat tgctaagata ttcttctctt
     3121 gtcagtgtat tattataaaa ctatttgata taattatagg tgaatattgg agttttccta
     3181 gcctataatt gggaaaggag taataatgat aacggtcaca aaaaaggatt tagttgagct
     3241 aggatatgga acttcttttt cagcagacat cataaaaaaa gcaaaagaat tgatgattac
     3301 aaaagggcat atctactatc aatctcgaaa gctagataga gtccctcgtg aagctgttga
     3361 agaaattcta ggaattcggt tttcaaatga aaacaggtct gagtagtttt tgcacagtaa
     3421 ggagtaaaaa ctatggttaa agacccaata aaaaaagcaa agaatggaac ctattatttt
     3481 cgtgcaaatc taggttatga ttctaaaggg aaaaaaattc aaaaatatcg tagtggattc
     3541 actactaaaa aagaagcaag agaagaatat tcgaaactgc tattgatgaa accagaagaa
     3601 ttaagtgaga ataaggataa aatgacgttt aaacattata tctttgaaat tttccttcca
     3661 tggtataaga cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac tattaagaag
     3721 catttctctt attttgataa aatggctgtt tccgatattg agcctattga tgtgcaaaat
     3781 tggcaactaa aattatcaaa gaagtgtaag tcttcttatg taagagctgt tcaaggacta
     3841 ttttcagttg ctatggatcg agcaattgtc cttgggataa ccaccacaaa tccatctaag
     3901 attattggca atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa agaagaattt
     3961 gaaaaagtaa tctctctaat ttacaaagaa gattactatc aacacttttt atttatctct
     4021 ctgtggtttt tatttatgac tggaatgcga attggtgaag ctactgccat tcaatgggaa
     4081 gatattgatt ttgactcagg agtactaacc atcaacaaaa cactctatta caagaatcaa
     4141 tcgaattata gatttgttga accaaaaaca aaggcgagcg ttcgtcatat tgtattagat
     4201 aaatgcacat taacttattt atccgaatgg aaggaagtac aacaaaatct aattcagaca
     4261 gactttgtaa tgagctataa cggtattcct acccaaaaac atacactatc atatgctatt
     4321 gaacgctatg ctaagctggc tggtatccat cgaatcagaa tccatgggtt acggcattct
     4381 catgcttcct tgttaatcag tatgggagaa aatccactta tcattaaaga tcgtctagga
     4441 cacgaagata ttgagacgac tttaggaacg tatggacatc tttatcctaa cagtaatttc
     4501 gaggtagcta acaaattaaa tggagccatt tcctttaaag aagcaaaaca aaattatgat
     4561 acttcaccaa agaatcaatt tactgtagac tacttgcgaa acaaagaaat gaaaaaagtg
     4621 caataaaagt gcagtaaaaa gataagaaga gccagaaacc cttggtataa gaggatttct
     4681 agctctccta caactattcc cactccactg ttgctggtgg tttagaagtg atgtcataca
     4741 cgatacggtt gacgtgggct acttcgttta cgatacgtac agagattttt tgcaagactt
     4801 cccatgggat acgggcaaag tcagcggtca ttccgtcgat tgaagttaca gcacggatac
     4861 cgactgtgta gtcgtaagta cgtccgtcac ccataacacc gacagagcgg atccctggaa
     4921 gaacagtgaa atattgccag atatcacggt caagtccagc agctgcgatt tcttcacgca
     4981 agatagcatc agattcgcgt acgatttcta gcttgtcttc agtgatttcg ccaagaacac
     5041 gaataccaag accaggacca gggaatggct gacgccaaac gattgaatca ggcatgccta
     5101 actgagtccc taattcacgg acttcgtctt tgaataatgt gtttaatggt tcgatcaatt
     5161 caaattgcat atcttctggc agtccaccta cattatggtg agatttgatc gtctgtgctg
     5221 tttctgtacc agattcgata acgtcagtat acaacgttcc ttgtgctagg aaagagacgc
     5281 cttcttcacc agcaagtttt gttgcttcgt catcaaaaac atatacgaat tcattcccaa
     5341 tgattttacg tttttgttct gggtcagata cacctttcaa cttgtctaag aagcgatctc
     5401 ttgcatcgac tttgatgatg ttcaagccga attttccgcc tagactttcc attacttgtt
     5461 ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat agatgtcaat tggtcaccga
     5521 tcgctttttg taaaagaacc ccaacgacac tagaatcaac gccacctgaa agaccaagca
     5581 agactttttt gtcaccaact tgttcacgga ttttttctac ttgcatatcg ataaagttat
     5641 ccattgtcca gttaccttca cagtgacaaa catcaaaggc aaagtggcgc agcaattcga
     5701 ttccgtattc ggaatgacgt acttctgcat ggaattggat accataaaaa ttacgtgttg
     5761 tgtcttggat agaagcaatc ggacaatctt tgcttgttcc gactgtttca aagccttcag
     5821 gaactttagt taccaagtca ccatggctca tccatactgt ttgttgtttt ggtgtttctt
     5881 taaacaagac agcttgatcg cttgttactt ctaacaaggc tttcccatat tcgcggtttt
     5941 tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca tagcaaatcc
     6001 ctaatacagg aatacctaaa ttgaagatct caggatcgat cccaaatgca ttctcatcat
     6061 atacactgtt tggtccgcca gaaagaatga ttccctttgg attcatcgct tttacttctt
     6121 ctgcagtaat gcggtggctc aataattcgg aaaagactcc aagatcacga atacgtcttg
     6181 tgatcaattg gttatactga ctaccgtagt ctaatacgat aatcttttca acgttcgtca
     6241 tatcggcaac gtttgtcaaa tttattaccc ctatcccttt aaattttatc tcaaaatagt
     6301 caaactattt taaaatgcta atattttcgc atgaaaatcg aatcgatgat atgattttca
     6361 gttttatgga gaattatatc ggctcttcct cgtgtaggca agatatattc ctgtaaattt
     6421 ttcaaattca catttttcca tacatctttt gccattttaa aggcttcttc acgatctccg
     6481 atagcaaaac gataataata attctctgga ttttggaaag ccgtatccag taacgcacca
     6541 aaacgatcca aataccattt ttcgatcagc acgggatcgg catcgacata gacagaaaaa
     6601 tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta attgaagcgt attgatcccc
     6661 tcaacgatca agatatcagg ctgctggatc aattcgtact tgtctggaat gatatcatac
     6721 acatcatgtg agtaaacagg tacttttatc tcttcctgac cagattttac acggttcaag
     6781 aaatcgatca gcatctccat atcataactt tcaggaaatc ctttacggtc catgattcca
     6841 cgctctttta atatacgatt tggataaaga aatccgtcag tcgtgatcag ctgaacgttt
     6901 cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc tggctgttgt actttttcca
     6961 acggctacac ttccggcgat cccgataata aaaggcggta cttctacgta tttatgaaga
     7021 aataaacctt tactcaagaa caaagattca aattctttca tataaagata aatcagatga
     7081 gcaagaggta catagatatc tcgaacatcc tgcatggaaa tgcggtcatt caaacttttg
     7141 atattatcta gttcatcttg tgtcaaaggt gcttgcccat tacgataaaa ttctcgccat
     7201 tcttctcttg ccacacggta gtagttcatt ggatcattca tattagcctc ctgcgttctc
     7261 ccatcaatgg aactatttta acacaaaata aacgcaaacc ccaccactta agagagattg
     7321 ccgatctttt tgtataatct ttataataga aagaaacgga gtcttattct tgacttgcgc
     7381 acggatacat aaaaggatag actgttgtca tgaaagaaat gagaaggatg gaagacatga
     7441 caaatcatta ctattctgaa cgcccagata cggcgcacga ttttgaacaa tgggcttttg
     7501 agttaaaagg cagaaccttt caatttgtaa cagattctgg tgtattttca cgtgaaacag
     7561 tggattatgg ttcacgtgtc ctgattgatg ctttcgattg ggaagagtta cctgatgaag
     7621 gaaagatctt agatgtggga tgcgggtatg gaccgattgg gttagctttg gcatttgcga
     7681 cacagcggtt tgttgaaatg gtcgacatta atgaacgagc ggtaagtcta gcgcaaggaa
     7741 acgcaaagcg taaccaaatc gaacatgttg atatccatca atcgaatatc tatgaagcag
     7801 ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc gatccgtgct ggaaaaaaag
     7861 ttgtacatga aattttgaca ggcgcatatc ctcggttgaa gaagggcggt acactgacaa
     7921 ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa gaaaatggaa gaaacatttg
     7981 gtaatgcaga aatcgtcaca aaagataaag gatattatat catcaaaagt gtgaaagaat
     8041 aactagctga aaaatagtgg agcgacgaaa gagtggaact gggcgtgcat gtacgggtac
     8101 gtatcagttc tttttttgtt gagaagtttt taaaaacaaa gaactatttt ttcttcaaaa
     8161 agaaatagta ggaacagagt cgttgttttt tacgataaaa taaataagat acagaacttg
     8221 tatagaaggc tgccaagtcg gtagataaag gaggttttta tcaaaaaaca tactttcctt
     8281 ttttctctat tcttttcttg ggggttttgg tacactgata gggaatttag atagaaggga
     8341 gccaatagga tgaagctgac catcagagat atcgcagaaa tggcaggcgt atcagtaaca
     8401 actgtctcac agattttgaa taacaaagga agcagattta gtgataagac aagaaaaaaa
     8461 gtgttgtcga tcgtgaatga acatcattat aagcccgatt atttcgcttc aaatgtgatc
     8521 aatcgacatt caaagaccat cggaatgatc gtccctgatg tcactgattt cttcttctcc
     8581 aaactgatcg aaggtgtaga gagctatttg aatcctttgg gctatgtgat catgttatgc
     8641 aattctcgtc atagccaaga aaatgagatc aagtatttac aagagctttc ccaccgctca
     8701 gtggacggaa tcttgctggc cacaccaaat attttacctg agaaatatgc attagacagc
     8761 ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg gattgaatcg tcgggataac
     8821 ggacggctta tcgtaaagga atatgagggc gcctatcaag ctgtttcgct gtttattgaa
     8881 aatggccata cgaagatcgg catgctgaag gaaaccactg gatactatca attagaagaa
     8941 cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc tgccttttaa tgggaaattt
     9001 gttgaacatg gagagttgac tgtccaaggt ggatatactg catcaaaaaa attactcaaa
     9061 cataaagata tcacggctat tttttgcggg aatgatgcca tggcgatcgg atgctatcag
     9121 gcgatcgatg aattgggcaa aaaaatccca gaagatatct cagtcatagg attcgatgga
     9181 ttaaagctgt cagaatacat gattccgcaa ttgacgacag tgcagcagcc aagttttgat
     9241 attggatttt atgctgcacg tttcctgatc gatacgatcg aatttcctca aagaaaagtt
     9301 ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga gcgtaaaagt attgacaaag
     9361 ggctaactcg gcggtatatt gcttatgtaa gcatttacaa aggctagtta gtgctttttt
     9421 tatttttagt aaaccagttt actaaagtgg tttacctttc ctaaaaataa tgtataatac
     9481 cgaatgaaga caaggaggat caggcaatgc gaatggtgga tctgattgaa aaaaagaggg
     9541 atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt cacgaactat acgaatggaa
     9601 agattccaga ttatcaagtc agtgcattat tgatggcaat tttttatcaa gacatgacaa
     9661 atgaagaaat cacgaactta acattggcga ttgctaattc tggtgatgtc atcgatttga
     9721 gttctcttga aggaataaag gtagataagc attcgacagg tggcgttggt gacacgacca
     9781 cattgatttt agccccccta gtagctagtg tcggagtgac agtggctaaa atgtctggac
     9841 gcggtctagg ttacacaggt ggtacactag acaagctaga agcaattccc ggatttcaga
     9901 tcgagctttc ggatgaagaa tttgtacgca ttgttaatga aagtaaagta gcggtaattg
     9961 gtcaatcagg aaacttggca ccggcggaca aaaaattgta tgccttgcgt gacgtcacag
    10021 cgaccgttga ttctcttccg ctgattgcca gttcgattat gagtaaaaaa atcgcggcag
    10081 gagcagatgc aatcgttttg gatgtgacca ctggagacgg tgctttcatg aagaacatcg
    10141 aagatgcaag acgtctggca aagaccatga cgagcattgg gaaactggct aaccgagaaa
    10201 cagtggcggt tatctcagat atgtcagaac ctcttggcga agctatcgga aatagtctag
    10261 aagttgtcga ggcaatcgaa acattacaag gaaatggtcc agaagatcta gtagaaatgt
    10321 gctacgcctt aggaagccaa atggttgttc ttgctggcaa agctaagaca accgatgaag
    10381 caagaacgtt gcttcaagaa gctctggaat caggtaaagc gttagcaaaa ttcaaagaaa
    10441 tgatccaaaa tcaaggggga gacccagcga tcgtggaaca tccagaacga attttgactg
    10501 cacgctacac aatggagctt cctgctaaac aaagcggagt tgtgtcaaaa atcgtggcaa
    10561 atgaattagg aattgctgca atgatgctag gtgctgggcg taaaaccaaa gaagaagata
    10621 ttgatcacgc tgttggattg aaactgcata aaaaaattgg tgacactgtg acaaaaggtg
    10681 agtcactatt gacgatctat agtaacgacg aagaaatcac ttctgtcatt gaattattat
    10741 ataaaaatat tgaaattggt gaatcggcaa tgaagccaac gttgattcat gacatcatca
    10801 cagaataaag gaaaaaagcg gactttaccg ttttaaagaa aaggggaaaa gaaatggaac
    10861 taaaccgaat gatcgaccat actattttga aagcagacgc atctaaagaa gctgtgatgc
    10921 aaattatcga agaagcaaaa aaataccatt tttattctgt ctgcatcaat ccagcatggg
    10981 ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc cgtatgtaca gtaatcgggt
    11041 ttcctctagg agcaaacaca cccgagacaa aagcatttga aacaacagat gccatcaata
    11101 acggtgcaga tgaagtcgat atggtcatca atatcggtgc tttgaaatca aaggatgatc
    11161 aacaagtcca aaaagatatc gaagcagttg ttgaagcagc aaaagatcgt gcgcttgtca
    11221 aagttattat tgaaacatcg ttattagata gagaagaaat catccgagct tgcgaaatag
    11281 caaaagctgc aggcgctgat tttgtcaaaa catcaactgg tttctcaaca ggcggagcaa
    11341 aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc agaaatgggt gtcaaagctt
    11401 ccggcggtat ccataacgaa gaagaagcaa tggcaatgat cgaagctggt gctacaagaa
    11461 tcggaacaag cgcaggagta gcaatcgtat ctggttcaac tggtgaaggc tattaatttt
    11521 ttcaaaggag aagctcatgg cagaactcaa acaagaatgg atcaacacag ctattgaagc
    11581 gttagataaa gcctatgttc cttattccca ttttccggta ggcgcttgtc tcgtaacgga
    11641 atcaggcaag atttatcaag gcatcaacat tgagaatgca tcatttggat taacgaattg
    11701 tgccgagcgc acagcttttt tcaaagcagt atctgaagga gaaaaaacat ttacgcatct
    11761 agtcgtagcg gggcacaccc ccgatcctat ctctccttgt ggtgcatgtc gacaagtaat
    11821 ggcagaattc tgttcccctg atatgccggt tacgctagta ggagacaatg gtgtgacgaa
    11881 ggcaacgacc gttcgtgcac tgctgcctta tgcatttact gaaaaagatc tataactcat
    11941 gttcattcag tcagctgact gttgaaaaat aaaacaatta ttttaggagg ctttacagaa
    12001 atgaaaaaag caaaattatt tggtttaggt gctgtcgcac tagcagcagg gttattttta
    12061 ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg gcaaaagctc atcagacaca
    12121 acaactgcag cattgatcac agacacaggt ggtgtagacg accgttcatt caaccagtca
    12181 gcttgggaag gactagaaaa atgggggaaa gatcaaggac tttcacgagg aaatgatggc
    12241 ttccaatatt tccaatcttc aaatgaatca gattacatcc caaatattga ccaagcattg
    12301 aatgctggat tcaaaacgat ttttggtatt ggttataaac taaaaccagc gatccaagaa
    12361 caagcaacaa acaatacagg tacgaacttt gttattatcg atgatgtcat cgatggatta
    12421 gataacgttg tatctgcaac tttcaaagac aacgaagctt cttatcttgc aggtgttgca
    12481 gcagcttata cgactgaaac aaacgtagtc ggctttatcg gcggtgtaaa aggtgaagta
    12541 atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg caggtgcgaa agaattagga
    12601 aaagaaatca aagtattgaa tcaatatgct ggtgacttct ctgctccaga taaaggacgt
    12661 tcaattgctc aaggtatgta tgcacaaaac gcagatatca tcttccatgc ttcaggcggt
    12721 acaggtaacg gtgtattcca agaagcaaaa tcattgaacg aatcaggcga caaaaaagtt
    12781 tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg aatacacatt aaacggcgaa
    12841 aagaaaaact tcacattgac atctacactg aaagcagttg gaacagtagt agaagatttg
    12901 gcacaaaaat cagcggacgg taaattccct ggcggcgaac acactgttta tggattgaaa
    12961 gaagacggtg ttggtctaac agaaggacaa ctttctgacg aagcgaaaaa agcagttgat
    13021 gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc cagaaactcc tgaagaaaac
    13081 taaaaccatt tctatagcct tgttagacac atgtttgaca aggctatggt ttgattagaa
    13141 caagaattgt tcatttatcc aagctgcctt gtgacagttt ggataaggga acaactccct
    13201 gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg tgaatcttag tcaatcgttg
    13261 ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt caagaaaata ttgtcataga
    13321 aatgcagaat attaccaaag aatttggaac attcaaggca aatgacaaca tcaatttaca
    13381 agtaaaggca ggagaaattc atgcgttgct tggagaaaac ggtgctggca aatctacatt
    13441 gatgaacgtg ctttccggat tattagagcc gacatcaggg aaaattttga tgcgtgggaa
    13501 agaagtaaag atcacaagtc cgaccaaagc caaccaatta gggattggga tggtccatca
    13561 gcactttatg cttgttgatg cctttactgt aacagaaaac atcgtgttgg gaagcgagcc
    13621 tagtcgcgca gggatgcttg accataaaaa agcacgaaaa gagatccaaa aagtttctga
    13681 acagtatgga ttgtcagtca acccagatgc ttacgttcgt gatatttcag ttggaatgga
    13741 acaacgggta gaaattttaa aaacacttta ccgaggagca gatgtactga tttttgatga
    13801 gccgacagct gtattgaccc ctcaagaaat tgatgaatta atcgtgatca tgaaggaatt
    13861 agtcaaagaa ggcaagtcga tcattttgat tacgcataag ttagatgaaa tcaaagcagt
    13921 agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc ggtacagtca acgttaaaga
    13981 cgtaacctca caacaattag ccgatatgat ggttggaaga acggtttcat tcaaaacgat
    14041 gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt gaaaatctag tggtgaaaga
    14101 aaatcgtgga ttagaagccg tgaaaaactt gaacttagag gttcgtgctg gcgaagtact
    14161 tggtatcgct ggaatcgatg gaaacgggca gtcggagttg atccaagctt tgactggttt
    14221 gcgaaaggca gaaagcggac atatcaagct aaaaggggaa gacatcacta ataaaaaacc
    14281 ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa gaccgtcata aatacgggtt
    14341 agtcctagat atgacattat ctgaaaatat cgcactacaa acctatcatc aaaaacctta
    14401 cagtaaaaac ggcatgctga attattcagt aatgaatgaa catgccagag aattgatcga
    14461 agaatatgat gttcgaacaa cgaatgaact tgttcccgca aaagctttat caggcggaaa
    14521 tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat cctgatctgt tgattgttgc
    14581 aaatccaact cgtggactgg atgtaggtgc gatcgaattt attcataaac gtctgatcga
    14641 acaaagggac aaatacaaag cagtgttact gatcagtttc gaattagaag aaattttaaa
    14701 tgtttcggat cgtattgctg ttatccatga aggagaaatt gtcggtatcg ttgatccgaa
    14761 agaaacatct gaaaatgaat taggtctatt gatggcgggc tataccttag aagaagcacg
    14821 aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg cagcgaaaaa ttccgtaatt
    14881 tattagtgcc tgtactttca gttgttttag gattgatctt aggcgcgata ttgatggctg
    14941 cttttggttt tcagcccatc caaggttatt cttctatgct agatgcagcc cttggttcgc
    15001 aaagagggat aggtgaaacg ttaaggcaag caacgccgtt gatttttaca gcattaggtt
    15061 tttctgtagc taactcagct gggtttttca atattgggct ttctggacaa gcactttgcg
    15121 ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga tctgccaaaa gttgttttgc
    15181 ttccgttatg cgtgatctta ggtgccttag ctggtgcgat ggcagcggct gttccaggtt
    15241 tacttcgagc attttttgga acaagtgaag tgatcgtaac aatcatgatg aactatatct
    15301 tattatacgt aagcacattt gctttacatg aagtgatgcc tgaaagctat cgctcaagtt
    15361 tggactcttc caatatgatc agtcaaaatg cctcactgcg gttaccatgg ttaacacaag
    15421 cttttggcgg ctctcgcgta aactcaggat tatttttagc aatcatcgcg cttattttcg
    15481 tttggctgct gatgaagaaa acgactttag gatttgaaat ccgttctgtt ggattgaatc
    15541 cttttgcttc tgagtatgca ggaatgagta gtaaacgtac gatcgttttg tcgatggtca
    15601 tttctggcgc tttggcaggg cttggcggtg tggttgaagg attaggaacg tatcaaaact
    15661 tctttgttca aacgacttcg ctatctattg gatttgacgg aatggctgtt tccttgcttg
    15721 gttctggatc agcaatcggt attcttttat cagcgatctt gttcagtatt ttgaagatcg
    15781 gtggacttgg catgcagaca ggagcaggtg ttccgtttga gatcgtcaat gtatcgattg
    15841 ctttgattat cttctttgtc gggatcaact tcctgattcg tttcgtcatg gctaaagtct
    15901 tccaagggaa aaaacaagaa gagatcgttg cgacaataga atcgaaacca acgaatcaaa
    15961 accatgaagg gggcgaacta taatgtcgac tatcgaatta attgccttga tccttacttc
    16021 tacactggtt tattcaacac cgctaattct aacgtcacta ggcggaactt tttctgaacg
    16081 aagcgggatc gtaaacgtag ggctagaggg aatcatggtc atgggtgctt ttagttccat
    16141 cgttttcaac ttgacgatga gtccatcact tggttctatg acaccatgga tcgctatttt
    16201 agtgggtggc gtcgtaggta tcctattttc tctgattcac gcagtagcaa cagtcagttt
    16261 gcgtgctgac catattatca gcgggacagt aattaatctg atggcacctg ctttaggtgt
    16321 tttcttgatc aaagcatggt atggtaaagg tcaaactgat aacatcacgc agaatctggg
    16381 gtatttttct ttcccaggat tagcagatat tccggtcatt ggaccaattt tctttaaaaa
    16441 tacattctta cctgcttatt gtgctatttt gattgctgtc ctagcttggg tgatcatctt
    16501 caagacgaaa ttcggcttgc gcctccgttc agttggtgaa aatcctcaag ctgccgacac
    16561 attaggtatc aatgtatatg ctatgcgtta ttcaggcgta ctgatttctg gcttgttagg
    16621 cggtatgggc ggtgctgttt ttgctcagtc gatttccggt aacttttcag ctggtacgat
    16681 cgttggacaa gggttcatct ccatggctgc tatgatcttt ggtaagtgga atcctttagg
    16741 ggcaatgggt gcctcattgt tctttggttt tgctcaaagc ttgagtatca ttggcgcaca
    16801 attaccagtt atttcttcga ttccgccagt attgctgcaa attgctccat atttgctgac
    16861 aatcattgtg ctggttgttt tcttagggaa agcttctggt ccaaaagcaa acgggaagaa
    16921 ctatatcaaa tcaaaataat cttttagcga ataaaaaggg tgggttacgg gaagtgtttt
    16981 tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa agaaagaagg aataatcatg
    17041 tttaaacgcg tacatctgat cgtcatggat tcagtaggaa ttggagaagc accagatgcc
    17101 gataagttcg gtgatgttgg ttctgataca ttaggacaca ttgcgaaaga agcaggattg
    17161 aacgttccac atttagagca gcttggttta ggaacgatcc gtcctcttga aggtgtggaa
    17221 tcagtgaaag atcatcaagg ctatgcgacg aaattagaag aagtttctgt agggaaagat
    17281 acgatgacag gccactggga aatcatggga ttgaatatca aaactccatt tcgtgtgttt
    17341 cctaatggat tccctgatga tctgatcaaa caaatcgaag aattttctgg ccgtaaagtc
    17401 gtatgtaaca aaccttatag cggaacagct gttattgacg attacggtga acaccaaatg
    17461 aaaacaggcg atttaattgt atatacttct gccgatcctg tccttcagat cgcggcacat
    17521 gaagaaatca ttcctttaga agaattatat cggatttgcc aattcgttcg tgatataaca
    17581 aaagatgatc cgtatatgat tggccgaatc attgcacgtc cgtacgtggg tgaaccagga
    17641 gacttcacac gaacaagcaa tcgccatgat tatgcgttga atccattcgg acacacagtc
    17701 cttgattctt tgaaagaagc aggaaaggac gtgattgctg ttggaaagat caacgatatt
    17761 ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa gcaatatgga tggcgttgac
    17821 cagctgctga aagttatgaa aaacgatttt tcaggaatca gctttaccaa tttagtagat
    17881 tttgatgcat tatacggtca tcgtcgggat gtagtcggct atgcgcatgc gattgaagaa
    17941 tttgacttac gaattccaga attgcttgaa gcgatggaag aagacgactt gctaatgatt
    18001 acagccgatc acggaaacga tccgacgttt actggtaccg atcatacgcg agaatatgta
    18061 ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg cattgcctca aggatattat
    18121 tcagatattt cagcaacgat tgctgagaac tttagtgttt cttcgactga aaatggcgaa
    18181 agcttcttga aattattaaa ataatctttt taaataggag aaagcaaatg accaagttaa
    18241 gcgagatgtt acaagaaacg acccagttta tccttgataa aggagtaaaa gaaattgatt
    18301 ttggtttgat tctaggttca ggattaggtg aattagcaga tgaaatcgaa gatgctgtaa
    18361 tgatttctta tgatgagatc ccgttctttc ctgtttcgac agtagttggg catgcaggaa
    18421 agctagttta tgggtctctg gcaggcaaga aagtgttagc catgcaaggg agattccact
    18481 actatgaagg tcattccatg caaacagtga cctatccagt acgtgtgatg gccggactag
    18541 gggcccattc agtcattgtg actaatgcat gtggtggtgt caacgaatca tttgtaccag
    18601 gagatttgat gctgattacc gatcatatca attttactgg tgaaaatcct ttgatcggtc
    18661 cgaatgaaga tgcaatgggc cctagatttc cagatatgag tcaagcttat acgcctgact
    18721 atcaagcaac agcaaaagca gcagcagaaa aactaggttt ggatcttaaa gaaggcgtat
    18781 atatgggcta ttctggacca acttatgaaa cgcctgcgga gatccgaatg tctcggacaa
    18841 taggagccga tgcagtaggg atgtcgactg ttccagaagt catcgtagca gctcacagcg
    18901 gattaaaagt gttaggaatc tcttgtatta ccaacttagc agcaggtatg caagcgaatc
    18961 tgagccatga agaagttgta gaaacaactc agagagtcaa acaatctttt aaagcattgg
    19021 ttaaagaaac attgacatta ttgtaatagg aggaacaata aaatgagtgt gcatattgaa
    19081 gcgaaagaag gcgagatcgc cgataagatt ttacttccgg gagatccttt gcgagctaaa
    19141 tatattgcgg agactttttt ggaagatcca gtttgttaca atcaggtacg cggtatgtta
    19201 ggctttaccg gcaaatataa aggagaacgt atttctgttc aaggaacagg aatgggaatg
    19261 ccatcagcca ccatctatgc ccatgaattg atccagtcgt atggtgtaaa gaaattgatt
    19321 cgagtaggta catgcggtgc attgtcaaaa gacgtccatg ttcgtgattt agtactagca
    19381 caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt tccaagcctt tcattttcct
    19441 ccaatcagtg atttcaatct attactgaaa gcatatgaga tagctaaaga aaagggctat
    19501 acggttcatg taggaaatgt tctttcggaa gattctttct acaaagatga tttgacagag
    19561 acgttccaat tagctgaact tggcgtttta ggagtggaaa tggaagcagc ggctctttat
    19621 tacttaggcg cgaaatacca tgtgcagaca ttaagtttga tgaccgttag cgaccatttg
    19681 atcaccggtg aagaaacaac agctgcagaa cgtcaatcta cgttcaatga aatgatcgaa
    19741 gtaggattgg aaacagcgat agcagaataa gaaggaaatg aaaaagctgc cctgcattaa
    19801 gtaataagaa catccaagca aaaatagctc ctcatgtttt tgacttggct ggacttatta
    19861 ttgcaaatgt aagcttttga acaccgttta ccaagaggtt gtgaatctgc cgtttaactc
    19921 tgagtcacaa ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg
    19981 tgaccgagaa gttggcagga gaaaaccgtt tatacgaaaa aagggactgt gacaaatcgt
    20041 gtcacagtcc cttttttata gtgtttttta taggtagtat tttttagcaa ctgttaagtc
    20101 atcatttaat tcataaacaa gtggttgacc tgttggaatt tcaagatcca tgatgtcttc
    20161 gtcagagata ccttcgatat gtttagctag ggcgcgtaat gagttaccat gtgcagctac
    20221 taaaacagtt ttgttgtcta gtaatgctgg agcaatttca tcttgccaga atggcaaagc
    20281 gcgttctaat gtcactttca agttttctcc accaggaata tcgcgtttgt ctaacattgc
    20341 gtaacgacgg tcatttgctg cagaaccttc gtcagtttct tccattaatg gaggaagagt
    20401 atcgtaagaa cgacgccaga tgtgcacttg ttcgtcgccg tatttttcag ctgtttcttt
    20461 tttgttcaaa ccttgtaatt taccatagtg acgttcgttt aagcgccatg atttgatttg
    20521 tggtacccaa agttgatctg aatattctaa gatcaagtta catgttttga ttgcacgagt
    20581 taaaacagaa gtgtaagcaa catcaaattc gattccagct tctttgattt tgcgtccgcc
    20641 ttcttttgct tcttcgattc cttctggtgc tagatttaca tcagcccaac cagtaaattg
    20701 gttcaatgca ttccattcac taagaccgtg acgagaaaaa actaattttg gcatgtttag
    20761 ctctcctttt aagataggtt tatatttctg aaattttcag ttctgtttta ttgtacaatt
    20821 ttttggaaga gatttccatc gaaaaattag ataagattct atgagaaaac aagtagagaa
    20881 tataaaaaaa cgaacagtgc ccacgcaact gttcgttttt aggtattaga tgcataattg
    20941 ctcgatttct ttttcaggta aattgatata tttataacat gttccaactg aaacaccgca
    21001 ttttgaagaa ataaattgga tcgtttcttt tttttcataa tagagacgtc ggattttttt
    21061 gatcgttttt gcatcgattt ttggtcttcc gccgattttt ccttttttac gagcttcatc
    21121 taatccaact aatgtacgtt ctttgatcaa tgcgcactcc atgtttgcta ggttttccat
    21181 taattggaaa tagatttttc ccatttcgcg cgtatcgatt ccttcatcaa gactaatcaa
    21241 gtgcagcccg cgatctttga ataattgcgt gagttctgtt aattgccgcg tagatttgcc
    21301 taatcgatga agttggcaaa tgactagtgc atcgccttgt tgcatgtcag cgatcactga
    21361 ctccaaatga acagcagtag aatcattttg ttccacttca tatgtctcat ggaaaatttc
    21421 ttgacaacca aagtcagata attgatcaac ctgagttgtt aaatcaacgt catttatggt
    21481 tgtgcgtgca taaccaataa ttttcatggt gattcctttc tagcagtgat tttctaactg
    21541 gatataacag taaaaatgta gcataaagta cgtcaaaaag atagtttttt cgcaaatttt
    21601 ccgctgtctt tttttattgg atgatcaagc agtttttctt tatttacgac ggattgcgtc
    21661 aagaatctca acgccttttt ctgagccgac aactacttta gaaaccatgt ttaggaaaag
    21721 cccatgttct actaccccaa caagttcatc taaataggta gctaagcttt ctggctgttc
    21781 gatgacttct agatgcagat caatgatgta atgaccacca tctgtggtta agacttctcc
    21841 gttttcgtct gtacgcaaga cgggttggaa tcttttttct tcaaaaatat gaagtagctg
    21901 ttgggagcca taagggatca cttcaactgg gagtgggaac ttcccaagtt tatcgactaa
    21961 tttagaagaa tcaacgatcc aaatcgtttc ttttgagtac gtagcgacta ttttctcgaa
    22021 aagcaaagca gcgccgccgc ctttgatccc ttgaaaatca gcactgattt catcagcacc
    22081 gtctattgtc aaatcgacta cggggacttc atcaatactc tttaacggta ttcctaattt
    22141 ttccgcttgt tcttttgtcg catttgaagt agtaactcct gtgatacgta aaccttcttc
    22201 acgcatacgg cgaccaatct cttctaccat ataataggcg gttgaaccgg tcccgagacc
    22261 tacgatcatc ccgtccttta cgaatttcgc ggcttcaatg ccagctaatt gttttaaatt
    22321 catctttgcg tccacctttt tttagtttga gcatcgtgct ttcctcacta ttttagcgtg
    22381 atttacggta aaaagatacc attctttttt attccaataa agctttttca aaaaaaattg
    22441 catctcttgt tgacatgttt tttcattcgt gatattctaa caaaggtgct ttatctacag
    22501 gtttctgtga agaaacgtgc tgactgttta tcaaagcata tttgataagg atgcagacat
    22561 tgcatttatt ttttatcaca aaaaaagaac cacctggatg tgtggaacta gacgcaaatc
    22621 gtgggaagga ggaaacaagg aatgcctaca attaaccaat tagtacgtaa acctcgtaaa
    22681 tcaaaggtgg aaaaatctaa ttcacctgct ttgaacaaag gatataacag ctttaagaaa
    22741 actcaaacta acgtaaactc acctcaaaaa cgtggggtag caactcgtgt gggtacaatg
    22801 acacctaaaa aaccgaactc agctttacgt aaatatgcac gtgttcgttt gtcaaactta
    22861 attgaagtta ctgcttatat cccagggatt ggtcataact tacaagaaca cagtgttgta
    22921 ttattacgcg gtggtcgtgt aaaagactta ccaggggtac gttaccatat cgttcgcggt
    22981 gctttagata ctgctggtgt taacgaccgt aaacaaagcc gttctaaata cggaactaaa
    23041 aaacctaaag cttaatttta attattaaac aaatgaaact tgcttctact atatagaagt
    23101 aattactatg aacaatattt cggaaggagg agttacggat gccacgtaaa ggtcctgttg
    23161 caaaacgtga tgttttacca gatcctattt ataactcaaa attagtaact cgcttgatca
    23221 accgtgtaat ggttgacgga aaacgcggga ttgcagctaa cattatctat aatgcctttg
    23281 acttgatcaa agaatctaca ggtaacgatc cattagaagt gtttgaacaa gcaatgaaaa
    23341 acgttcaacc tgttcttgaa gttaaagcac gtcgtgttgg gggttcaaac taccaagtac
    23401 cagttgaagt tcgccctgaa cgccgcgtta ctctaggtct tcgttgggta gtaaactacg
    23461 ctcgcttgcg cggtgaacac acaatggaag aacgcttagc aaaagaaatc atggatgctg
    23521 caaacaacac aggtgcttct gttaaaaaac gtgaagacac tcataaaatg gctgatgcca
    23581 accgtgcgtt tgctcactat cgctggtaag attctctctg tttggtcggc ttttataagt
    23641 cgacctcaga tagtgtcctt atttttagca actagatagt aaagagagga gaaacaagga
    23701 gaatgaccag agaattttct ttacaaaaca ctcgtaatat cggtatcatg gcccacatcg
    23761 atgctggtaa aacgactgct actgaacgta tcttgtacta tactggtaaa atccataaaa
    23821 ttggtgaaac tcatgaagga gcttcacaaa tggactggat ggaacaagaa caagaacgtg
    23881 gtattacgat cacttctgct gctactacag cacaatggaa gggtcaccga atcaacatca
    23941 tcgacacacc aggacacgta gacttcacag ttgaagttga acgttcattg cgtgtacttg
    24001 atggtgctgt aactgttctt gatgcacaat caggtgttga acctcaaact gaaacagttt
    24061 ggcgtcaagc aacaacttat ggtgtacctc gtatcgtatt tgcaaacaaa atggacaaga
    24121 tcggtgctga cttcttatat tcagtatcaa ctttacatga ccgtttgcaa gcaaacgctc
    24181 acccaatcca attaccaatt ggtgcggaag acgacttcac aggaattatc gaccttgtaa
    24241 caatgaaggc tgaaatgtat acaaacgatc ttggaacaga gatcgaagaa acagaaattc
    24301 ctgaagaata ccgcgaatta gctgaagaat ggcgcgaaaa attggttgaa gctgtagctg
    24361 aaactgatga agaacttact ttgaaatact tagaaggtga agaaatcact gaagctgagt
    24421 taaaagaagg tatccgccgc gcaacagtta acgtagaatt ctacccagta ctatgtggtt
    24481 cagcatttaa gaacaaaggg gttcaattac tattggacgc tgttcttgat tacttgccat
    24541 caccgttaga tatcccagct atcaaaggga ttgaccctaa aactgatgaa gaagtagaac
    24601 gtcctgcaga cgacagcgct cctttctcag ctttagcatt taaagttatg actgacccat
    24661 tcgtaggtcg tttaactttc ttccgtgtgt actctggtgt tttacaatca ggttcatacg
    24721 tacaaaacgc aacaaaaggc aaacgtgaac gtgttggtcg tatcctacag atgcatgcca
    24781 actctcgttc agaaatctct gaagtttacg ctggagatat cgctgctgct gttggtttga
    24841 aagacacaac tactggggac acactatgtg atgaaaagag tctagttatt cttgaatcca
    24901 tggaattccc agaaccagtt atccaagtcg ctattgaacc taaatcaaaa gctgaccaag
    24961 ataaaatggg tgtggcttta caaaaactat ctgaagaaga tcctactttc cgtgctgaaa
    25021 caaacgtgga aacaggcgaa acaatcatcg ctggtatggg tgaacttcac ttagatatca
    25081 tcgttgaccg tatgcgtcgt gaattcaaag ttgaagctaa cgtaggtgcg cctcaagtat
    25141 cttaccgtga aactttccgt gctggcactc aagccgaagg taagtttgta cgtcagtctg
    25201 gtggtaaagg tcaatacggt cacgtatgga ttgaatttac accaaatgaa gaaggtgctg
    25261 gtttcgaatt tgaaaatgcg atcgtcggtg gggttgttcc tcgtgaatac attccagctg
    25321 ttgaaactgg tttgaaagat gctatggaaa atggtgtctt agctggctat ccattagtag
    25381 atatcaaagc taaactttac gatggttcat accatgatgt cgattcaaat gaaactgcct
    25441 tccgtgttgc tgcttctatg gcattacgtg cagcagctaa gaaagctaat ccagtaatcc
    25501 ttgaaccaat catggctgta gaagttgtta tccctgaaga ttatttaggt gatgttatgg
    25561 gtcacgtaac tgctcgtcgt ggacgtgttg aaggtatgga agctcgcgct ggcggccaac
    25621 aagttgttcg tgcgatggtt ccacttgctg aaatgtttgg ttatgctaca actcttcgtt
    25681 ctgctactca aggtcgtggt acatttacaa tgacatttga ccactacgaa gatgtaccaa
    25741 aatcagtaca agaagaaatc attaagaaaa acggcggaaa agctgaataa tttcttaaaa
    25801 ccggatagtc ctgcaaagag attatccaaa gagactatat ttttaatata gtttcgtgta
    25861 aaataattat cgatgatatg ggcatgtggt gtaccttgtg tacatcatat acctatcaga
    25921 caaataaaaa atatattgta tttttaggag gacattttaa aatggcaaaa gaaaaatttg
    25981 accgttctaa accacacgtt aacattggta ctatcggaca cgttgaccat ggtaaaacta
    26041 cattaacagc tgcaatcaca actgtactat ctaagaaaaa cggcggccaa gctatggctt
    26101 acgatcaaat cgatggtgct ccagaagaac gcgaacgcgg aatcacaatc tcaacagctc
    26161 acgttgagta tgaaacagat acacgccact acgctcacgt tgactgccca ggacacgcgg
    26221 attacgttaa aaacatgatc actggtgctg ctcaaatgga cggagctatc ttggtagttt
    26281 ctgctgctga cggcccaatg cctcaaactc gtgaacacat cctattgtct cgtcaagttg
    26341 gtgttcctta catcgttgta ttcttgaaca aagtagacat ggttgatgac gaagaattac
    26401 tagaattagt tgaaatggaa gttcgtgacc tattaacaga atacgaattc cctggtgacg
    26461 atgttcctgt agttgctgga tcagctttga aagctctaga aggcgacgct tcatacgaag
    26521 aaaaaattct tgaattaatg gctgcagttg acgaatacat cccaactcca gaacgtgaca
    26581 acgacaaacc attcatgatg ccagttgaag acgtgttctc aattactgga cgtggtactg
    26641 ttgctacagg tcgtgttgaa cgtggacaag ttcgcgttgg tgacgaagtt gaagttgttg
    26701 gtattgctga agaaacttca aaaacaacag ttactggtgt tgaaatgttc cgtaaattgt
    26761 tagactacgc tgaagctgga gacaacattg gtgctttact acgtggtgtt gcacgtgaag
    26821 acatccaacg tggacaagtt ttagctaaac caggtacaat cacacctcat acaaaattct
    26881 ctgcagaagt atacgtgttg acaaaagaag aaggtggacg tcatactcca ttcttcacta
    26941 actaccgtcc acaattctac ttccgtacaa ctgacgtaac aggtgttgtt gaattaccag
    27001 aaggaactga aatggttatg cctggcgaca acgtaacaat ggaagttgaa ttaatccacc
    27061 caatcgctat cgaaaacggt actaaattct caatccgtga aggcggacgt acagttggtg
    27121 ccggtgtcgt tacagaaatc aaagcttaat tcacttaagt tttaaagtaa agcaacattt
    27181 attcggacgt aatcgctcgg aacgaaggtc ttaggatctt tgttccgagc ttttttattt
    27241 attgcaggat caggaaaaaa gacaggagta ttataaatag aagaaacacg aaataaaaga
    27301 gtcagtattt tctaaatgaa actttatgaa aacaaaatgt tttttacaag aaaaagcctt
    27361 gctttgtgag tttatatata ttataatgac aaaggtgcgt ttactttagt gaattgcaca
    27421 gacgtaatca gaatgatttc gtcggctaag aaacgagagg ttgcgacacg cccggacgct
    27481 ttgccacgaa cgagcgtgac ggaaattttc gtggagctat gtctactttt caaaataggc
    27541 gaaggaggga acaagatggc aaaacaaaaa attcgtatcc gtttaaaagc gtatgaacac
    27601 cgtattttag atcaatcagc ggataaaatc gtggaaactg caaaaagaac tggagctagc
    27661 gtgtctggtc caattccatt accaactgaa cgcagtcttt acacaatcat ccgtgcgact
    27721 cataaataca aagattcacg cgaacaattc gaaatgcgta cacacaaacg tcttatcgac
    27781 attgtgaacc caacaccaaa aactgttgat gctttgatga agctagactt accatctggt
    27841 gtaaacatcg aaatcaaatt ataaaactaa aacaaaaata atggaggtgt actcatgacc
    27901 aaaggaatct tagggaaaaa agtgggaatg acacaaatct tcactgaatc tggtgaatta
    27961 attccagtaa ctgttgtaga agctactcca aacgtagtac tacaagtgaa aacaatggaa
    28021 acggatggct acgaagctat tcaagttggt taccaagata tgcgtgaagt tttatcaaac
    28081 aaacctgcga aaggtcatgt tgcaaaagca aacacggctc ctaagcgctt cattcgtgaa
    28141 ttcaaaaatg ttgagctagg agaatatgaa gtaggaaaag aaattacagt agatgttttc
    28201 caagcaggag acattattga tgtaacaggt actacgaaag gtaaaggatt ccaaggggtt
    28261 atcaaacgcc acggacaaag ccgcggacca atggctcacg gttctcgtta ccatcgtcgt
    28321 cctgggtcaa tgggtccagt tgcacctaac cgtgtattta aaaacaaacg tcttgctggc
    28381 cgtatgggtg gcaaccgcgt aactatccaa aacttggaag ttgtacgtgt cgatgcagaa
    28441 agaaatgtta tcttgatcaa aggtaatgtt cctggagcta aaaaatcttt aattactatc
    28501 aaatcagctg tgaaagctaa ataattgtag gaagaaagga ggaactaagg aatgccgaat
    28561 gtagcattat tcaaacaaga tggaagccaa aacggtgaaa tcacacttaa cgaagaaatc
    28621 ttcggaattg aacctaatga aagtgttgtt tacgatgcaa tcgtaatgca acgtgcgtca
    28681 ttaagacaag gaacacacgc ggttaaaaac cgtagcgctg ttcgcggcgg cggacgtaaa
    28741 ccatggcgtc aaaaaggaac aggtcgtgca cgtcaaggat ctatccgctc accacaatgg
    28801 cgtggaggtg gcgtagtctt tggaccaaca ccacgttctt acagctacaa acttcctaaa
    28861 aaagttcgtc gtttagcaat gaaatctgta ttgtcagaaa aagttgctga aaacaacttg
    28921 gtagcaatcg aaggcttgaa cttcgacgca ccaaaaacaa aagaattcaa acaagttctt
    28981 gctaacttat ctatcgactc taaagtattg gttgttctag aaaaaggtaa cgactttgct
    29041 gctttatcag cacgtaactt accaaacgtt tctgtagtaa cttctgataa tgtgagtgtg
    29101 ctagacgttg tatcaaatac aaaagtgtta gcaacacaaa ctgctcttac tcaaattgag
    29161 gaggtgcttg cataatgaac ttactagacg taatcaaacg cccagtgatc actgaaaaat
    29221 caatgcttgc catggatgac aagaaataca cttttgaagt ggacactcgc gcaaacaaaa
    29281 ctttagtaaa acaagcggta gaagctgctt ttgacgttaa agtcaaaaac gtgaacatcg
    29341 tgaacgtacg tgcgaaattc aaacgtatgg gcaaatatgc aggatataca aaaaaacgtc
    29401 gcaaagcgat cgttacattg actgaagatt caaaagaaat ccaattattc gaagctgctg
    29461 aataagcact aatagataac tcattaaatt aggagggaaa aacgtggcga ttaaaaagta
    29521 caaacctacc tcaaatggtc gtcgtaacat gacaggttct gattttgctg aaatcacaac
    29581 atcaacacct gaaaaatcat tgttacaacc attgaaaaac catgctggac gtaacaacaa
    29641 cggccgcatc actgtacgtc atcaaggtgg cggacacaaa cgccaatatc gcgtgatcga
    29701 cttcaaacgt aacaaagata acgttgttgc aactgttcaa acgatcgaat atgatccaaa
    29761 ccgttcagct aacatcgcgt tagtacacta tgaagatgga gtgaaagctt acatcttagc
    29821 acctaaagga ctagaagtgg gtatgaaact tgtttcaggt ccagaagcag atatcaaagt
    29881 cggaaatgct cttccattag aaaacattcc agttggtact gttatccaca acattgaaat
    29941 gaaacctggt aaaggtggac aattgatccg ttctgctggt acaagtgctc aagtacttgg
    30001 taaagaaggc aaatacgtat tgatccgctt gaactctggt gaagttcgta tgatcttagc
    30061 tgcatgccgt gctacaatcg gttctgtagg taacgaacaa cacgaattaa tcaacatcgg
    30121 taaagctgga cgctctcgtt ggatgcgtaa acgcccaact gtacgtggta gcgtaatgaa
    30181 ccctaatgat cacccacacg gtggtggtga aggtaaagct cctatcggac gtaaagcacc
    30241 agtttctcct tggggtcaac cagctcttgg ttacaaaact cgtaacaaaa aagctaaatc
    30301 agacaaactt attgttcgtc gtcgcaaaaa taaataatta ctttgaccat gtgtcgcaca
    30361 ttttgagagg aggttcacca tgggtcgtag tttaaagaaa ggaccttttg tcgatgagca
    30421 tttaatgaaa aaagttgaag ctcaagcagg ggttgaaaag aaaaaagtta tcaaaacttg
    30481 gtcacgccgt tcaacagttt tcccaagttt tgttggatat accatcgcag tttatgatgg
    30541 acgtaaacat gtaccagttt acatccaaga agacatggta ggacacaaat taggtgaatt
    30601 tgcacctaca agaacttatc gtggacatgc tgctgacgat aagaaaacga aacgctaatt
    30661 tgaggggagg ataaatccaa atggcagaac aaatcacatc agctaaagca actgcaaaaa
    30721 cagttcgcac ttcacctcga aaagcccgtt tagtaattga ccttatcaga ggtaaaagtg
    30781 tcgcagatgc aatttcaatc ttgaaattca caccaaacaa atctgctgga atcattgaaa
    30841 aagtattgat gtcagcagtt gcgaatgcag aaaataactt tgacttagat gttgaaaact
    30901 tggtagtaag cgaagctttt gttaacgaag gacctacaat gaaacgtttc cgtcctcgtg
    30961 caaaaggatc agcttctcca atcaacaaac gtacaagtca tatcacggta gtcgtatcag
    31021 aaaaataagg agggataatc agtgggtcaa aaagtacatc caattggaat gcgtgtaggc
    31081 atcatccgcg actgggatgc taaatggtat gctgaaaaag agtatgctga gttcttacac
    31141 gaagatttaa gaatccgtaa atttatttca actaaacttg ctgatgctgc tgtatcaaca
    31201 gttgaaatcg aacgcgcaac aggtcgcgta aacatttcaa ttcacacagc taaaccaggt
    31261 atggttattg gtaaaggcgg ttctgaagtc gaaagcctaa gaaaagaatt aaacaaatta
    31321 actggtaaaa gaatccatat caatatcgtg gaaatcaaaa aaccagatct agatgcaaaa
    31381 ttagtaggtg aaggaatcgc acgtcaatta gaaaaccgtg ttgctttccg tcgcgctcaa
    31441 aaacaagcca tccaacgctc aatgcgtgct ggcgctcaag ggatcaaaac tcaagtatct
    31501 ggtcgtctaa acggagcaga tatcgctcgt tcagaaggat actcagaagg aactgttcct
    31561 cttcatactt tacgtgcgga cattgattac gcatgggaag aagcagatac aacatacgga
    31621 aaactaggag ttaaagtgtg gatctatcgt ggagaaattc ttccaactaa aaaaaacact
    31681 gagaaaggag ggaaataatc atgttagtac ctaaacgtgt aaaacaccgt cgtgaattcc
    31741 gcggaaaaat gcgcggggaa gctaaaggcg gaaaagaagt agcattcggt gaatacggtt
    31801 tgcaagctgt tgattcacat tggatcacaa accgccaaat cgaagctgct cgtatcgcaa
    31861 tgactcgtta catgaaacgt ggtgggaaag tatggattaa aattttccct cacaaatctt
    31921 atactgccaa agcaattggg gtacgtatgg gttctggtaa aggggcacct gaaggatggg
    31981 ttgcaccagt aaaacgtggt aaaatcatgt ttgaaatcgc aggcgttcct gaagaagtag
    32041 ctcgtgaagc gttacgtcta gcttctcaca aattaccaat gaaaactaag atcgtaaaac
    32101 gtgaggaaat gggtggtgaa tcgaatgaag gttaaagaaa tcagagaatt aaccactgcc
    32161 gaaatgcttg atcaagaaaa acaattaaaa gaagaattgt ttaatcttag attccaatta
    32221 gcaacaggtc aattagaaaa cactgcacgt attaaagaag tacgtaaatc gattgcacgc
    32281 atcaaaactg tgttgcgtga acaagctaag taattatgga aggaggccat taagcgtatg
    32341 actgaaggaa gaaatcaacg caaagtttat caaggtcgcg tggtttcaga caaaatggat
    32401 aaaacaatta ccgttgctgt cgaaacaaag aaaaaccacc ctatctatgg caaacgcatg
    32461 aactattcta agaaatacaa agtgcatgat gaaaacaaca cagcaaaagt tggcgacatc
    32521 gtgagaatta tggaaactcg tccattatca gctacaaaac gtttccgttt gttggaagta
    32581 gtcgaagaag cagttattat ctaataacac gagattttcc tatatagatt gaatgcaaaa
    32641 tctgaaagga ggatacacaa cgtgatccaa gcagaaagtc gtttaaaagt cgctgataac
    32701 tcaggcgcac gtgaaatttt gacgatcaaa gtccttggtg gatctggtcg taaaactgct
    32761 aacatcggtg atgtgatcgt tgctacggtt aaacaagcaa cgccaggtgg ggttgtcaaa
    32821 aaaggtgacg tcgttaaagc cgttatcgtt cgaacaaaat caggagctcg tcgtacagac
    32881 ggttcatata ttaaatttga tgaaaatgct gcagtgatta ttcgtgatga taaaagccct
    32941 cgtggaacac gtatctttgg gccagttgca cgtgaactac gtgaaaacaa cttcatgaag
    33001 atcgtttcac tagcaccaga agttttataa tcttgagaca cttatcaaag gaggtgcgaa
    33061 acagttatgt ttgttaaaaa aggcgataaa gttaaagtta tcactggaaa agacaaaaac
    33121 aaagaaggcg ttgtactagc agcgttcccg aaaaaagata aagttattgt cgaaggtgta
    33181 aatgtcgtta aaaaacacca aaaaccttct caagctgctc ctcaaggtgg aattgttgag
    33241 atggaagcgc caattcatgt ttctaacgtg atggtggttg actctactgg tgtagctggc
    33301 cgagttggct acaaagaagt agacggcaaa aaagtccgtg tttctaaaaa aaccggtgaa
    33361 gttttagata aataaaagtt taaggaagga gggtcttact gaatgaaccg cctaaaagaa
    33421 aaatatctta aagaagtaac tccatcattg atggaaaaat ttaactatag ctctgttatg
    33481 caaacaccta aagttgaaaa aatcgtcatc aacatgggtg ttggtgatgc ggtatcaaac
    33541 gctaaaaact tagataaagc agttgaagaa ttaactttga tcactggtca aaaaccaatg
    33601 attacaaaag ctaaaaaatc aatcgctgga ttccgtttac gtgaaggaat gccaattggt
    33661 gcaaaagtta cattgcgcgg agaaagaatg tacgaattct tagacaaatt ggtttctgtt
    33721 tcactaccac gtgtacgtga cttccatggt gtaagtaaaa aagcctttga cggtcgtgga
    33781 aactatactt taggtattaa agaacaattg atcttcccag aagtagatta tgatttagta
    33841 gataaagtac gtggtatgga catcgttatt gtaacaacag caaactctga tgaagaatct
    33901 cgtgagttgt tgacacaatt aggcatgcca ttccaaaaat aattaaagga ggcgaactac
    33961 gtggctaaaa aatcaatgat tgctaaaaac aaacgtcctg caaaattttc aacacaagct
    34021 tatactcgtt gcgaacgttg cggacgtcca cattcagttt atcgtaagtt ccatctttgc
    34081 cgtatttgct tccgcgaact tgcctataaa ggtcaaattc ccggcgtgaa gaaagctagc
    34141 tggtaaaaag taaattcgca taagaaggag gtaaaagatc aatggtcatg acagatccaa
    34201 ttgcagattt tctaactcgt atccgtaacg caaacatggt taaacatgag tctttagaag
    34261 tacctgcatc aaaaattaaa cgtgacatcg ctgaaatctt gaaacgtgaa ggtttcgttc
    34321 gtgatgttga atacatcgaa gatgacaaac aaggcgtgat ccgtgttttc ctaaaatttg
    34381 gtaaaaacgg cgaacgtgtt atcacaaact taaaacgtat ctctaaacca ggtttgcgtg
    34441 cttatgtcaa agctgacgaa gtaccaaaag tattgaacgg tttaggtatc gcaatcattt
    34501 cgacttctga aggtgttgtc actgataaag aagctcgtga aaaaaatatc ggcggcgaag
    34561 ttatcgctta cgtatggtaa tcaagtagaa tctacaagga ggtgtcttta agtgagccgt
    34621 atcggaaata aagtcgttgt tatccctgaa ggagtaacag ttactcaaga cggaaacaac
    34681 gttacagtta aaggaccaaa aggtgaattg actcgtactt tttcagcaga tatcaaaatg
    34741 aatatcgaag gaaacgaagt aactttcact cgtccaaacg acagtaaaga aatgaaaaca
    34801 attcatggaa caactcgtgc gaacttcaac aacatggttg taggtgtcag cgaaggtttc
    34861 caaaaagcgt tagaactaat cggggttggg taccgtgcac aattacaagg atcaaaactt
    34921 gtattgaacg ttggttactc acatccagta gaaatcacac caccagctgg agtaactgtt
    34981 gaagtaccag ctaacacaca agtaattgtt aaaggtgcga acaaagaaga agttggcgaa
    35041 ttagctgcaa acattcgtgg aactcgtcct ccagaacctt ataaaggtaa aggtattcgt
    35101 tatgttggcg aatatgtacg ccgtaaagaa ggtaaaactg gtaaataatc gtttagaaaa
    35161 attgcataga agccagcagc aggaaaaagg catttggctg aagtttacct tcaaaaaaat
    35221 acatttttac ttgtgccgca gcttctattt ttcgaaacgt atttactgct tgaaatagta
    35281 gtacaataaa aataaagagg tgacaattgt gattacaaaa ccagataaga acaaaacacg
    35341 tcaaaagaga catcgtcgtg tacgtaacaa tatctctggt actgctgagc gcccacgctt
    35401 gaacgttttt cgttctaaca aaaacatcta cgctcaaatt attgatgacg tagcgggtgt
    35461 aacgctagca agtgcctcta ccttggataa agaaatttca ggtggaacaa aaacagaagc
    35521 agcacaagct gtcggtaaac tagttgctga acgtgcagct gaaaaaggca ttaaagtagt
    35581 agtctttgac cgtggtggat acctttacca tggccgtgtg caagctttag ctgaagctgc
    35641 tcgcgaaaat ggactagaat tttagaagaa ggaggaatac cattcatggt ttatatcgat
    35701 ccaaaacatt tggaattaga agaccgcgtt gttgcaatta accgtgtaac aaaagttgtt
    35761 aaaggtggac gtcgtctacg ttttgctgct ttagttgttg tcggtgacaa aaacggacac
    35821 gtagggtttg gtacaggtaa agctcaagaa gttcctgaag caatccgtaa agcaattgaa
    35881 gatgcgaaga aaaacttagt tgaagtacct atggttggtt caacaatccc acacgaagta
    35941 atcggtgctt ttagtggtgg tagaatccta atgaaacctg ctgtagaagg ttctggggtt
    36001 gctgctggtg gaccagttcg tgccgtatta gaattagctg gggtagcaga tatcacttct
    36061 aaatctttag gctcaaatac accaatcaac gttgttcgcg caacagttga aggtttgaaa
    36121 caattgaaac gtgcagaaga agttgcagaa cttcgcggca aatcagttga agaattaatc
    36181 ggataaggag gacaacaata atggctgaat taaaagttac tttaaaacgc agtattatcg
    36241 gacgtcctca aaaccaacgc gatacagtta aagcgttagg tcttacaaag attaacagtt
    36301 cagtggttaa gccttctaat gaagcaatta aaggcatgat caatactgtt tctcacctag
    36361 tggacgttga agaaatttaa tttagaaaaa tcataactag aacattaagg aggtgccaaa
    36421 cctatgaaac ttcatgaatt aaaacctgct gaaggttcac gccaagtacg taaccgtgtt
    36481 ggtcgtggta cttcatctgg taacggaaaa actgccggac gcggacaaaa aggacaaaaa
    36541 gctcgttcag gcggcggtgt gcgtttagga tttgaagggg gacaaacacc attgttccgt
    36601 cgtttaccaa aacgaggatt cacaaacgtg aaccgtaaag actatgcagt cattaatctt
    36661 gatgtattga accgctttga agacggagct gaagtaactc ctgttgcttt agttgaagct
    36721 ggaatcgtga aaaacgaaaa agctggaatc aaagtattag ctaatgggga actaaacaag
    36781 aaattaactg tgaaagcagc aaaattctct aaagcagctc aagaagcaat tgaagcggct
    36841 ggtggatcaa tcgaggtgat ctaatgttta gactattaaa agatgctttt aaagtaaaag
    36901 acattagatc gaaaatccta tttactgtat ttgtgttgtt tgtatttcgc ctaggtgcac
    36961 atattactgt accaggcgta aatgctgctt cattgcaaaa cctttcttct ttgccatttt
    37021 tcaacatgtt gaacttggta agtggtagtg cgatgcaaaa cttttccatt ttctcaatgg
    37081 gggtttcacc atacattact gcttctatcg tcattcagct tcttcaaatg gacatcgtgc
    37141 ctcgatttgt tgaatggtcg aaacaagggg aagttggtcg taaaaaactg aatcaagcga
    37201 ctcgatatct gacattagta ttggcattta tccaatctgt cggtatcact gctggattca
    37261 atcaatacgc tcaattaggt ttcgtgaata atcctaacgt agcaacttac gtaacgatcg
    37321 gtttgattct aacagccggt acgatgtttg ttacttggct aggagaacag atcacagaaa
    37381 aaggtatcgg aaatggcgta tcaatgatca tttttgcagg tatcatttct agattgcctg
    37441 aaagtatcag agaaatttat gaagattact ttataaatat tgatccttca gacatatgga
    37501 aatctgctat ttttgtggcc attttactag tagcggtctt gttgatcgtg acattcgtaa
    37561 catttttcca acaagctgaa cgcaagatcc cgatccaata tacgaaacgc gtagctggcg
    37621 cgcctacaag cagctatctg ccgttgaaag taaatgcagc tggagttatt ccagttatct
    37681 ttgctagttc gtttattgct acgccaaatg ctgttttaca agcattgagc ggtagattcg
    37741 gttctgaaaa ctggtacaaa gtgatgatgg agatctttaa ctataataca gcacctggtg
    37801 ctacgattta tacgattctg atcgttgcct tcactttctt ctacgccttt gttcaagtaa
    37861 accctgagaa attagcggaa aacttgcaaa agcaaggaag ttatatacca agcgtacgcc
    37921 ctggtaaggg gactgaagag tatgtctcaa gattactgat gcgtttaagt acagttggtg
    37981 cagtcttcct tggtttagtg gcattgcttc caatcattgc tcaaatggtt tgggatcttc
    38041 cacaatcaat cggattaggc ggtacaagct tactgatcgt tatcggtgta gctttagaaa
    38101 ctgcgaaaca gttagaaggc ttaatgctga aacgtaaata tgttggattc attgagtaag
    38161 aaagcgtgtt gaggtttccc ctcaacacac gttctcacac atttattagg agggaaaaca
    38221 atgaacctca ttttaatggg gctgccaggc gcaggtaaag gaacacaagc tgaaaaaatc
    38281 attgatgctt atggtattcc gcacatctca acaggagata tgttccgcgc agcgatgcaa
    38341 aacgaaacag ctcttggcct ggaagcaaag tcttatatgg ataaaggtgc attagttcct
    38401 gatgaagtaa caaatggaat cgtgaaagag cgactagctg aaccagatac agagaaaggc
    38461 ttcttattag atggctttcc acgtacgcta gatcaagcaa aagctctgga cgcaatgcta
    38521 aaagatttga ataaaaaaat tgatgctgtc atcgatatcc atgttggtga agaaatcttg
    38581 gtcgaacgtc tagccggccg atttatctgc agcaattgtg gagcaactta ccataaaatt
    38641 ttcaacccta caaaagttga agacacatgt gatcgttgcg gtgggcatga attctatcaa
    38701 agagaagatg ataaacctga gacggttaaa aatcgtctgg ctatcaacat caaaaatagt
    38761 gaaccaatct tggcttatta taaagaacaa ggtttgctaa acactattga tggtgctcgt
    38821 gaaatcgatg ctgtatttgc agatgtacaa aaaatcattg aaaaatagca cgattgcccg
    38881 tagccgactt gcattatgca gttactcttg tcaagttcaa taaattatga tagaatatgt
    38941 cagtccgact tctagagaat cctctagatg atccattttg agaattttga ggtgggaact
    39001 gcagtttccg ccattttatc gtgtgaaaat gcgaaacaag tacaaggagg tactgtgcgt
    39061 ggcaaaagaa gatatgattg aagtcgaagg tacagtcgtc gaaactttgc cgaatgcaat
    39121 gtttaaagtt gaactagaaa acggacacca agttcttgct actgtttcag gtaaaattcg
    39181 tatgcattac atccgtatct tacctggtga caaagtaacg gttgaattgt ctccatacga
    39241 tcttaaccgt ggccgtatta cttatcgctt taaataattg tactccgtaa aactacagga
    39301 ggtattatca tgaaagtaag accatcagta aaaccaatgt gcgaacattg taaagtaatt
    39361 cgtcgtaaag gacgcgttat ggtgatttgc ccagcaaatc caaaacataa acaacgtcaa
    39421 ggataattgg aggtgctata aatatggctc gtattgcagg agtagatatt cctcgtgata
    39481 aacgtgtagt agtttcactt acttatatct atggtattgg taacactact gctaaaaaga
    39541 tcttagctga tgctggtgta tctgaagatg ttcgcgttcg tgaattaacg aatgaacaaa
    39601 cagatgctat ccgtgcagaa gtagataaat taaaagttga aggggatctt cgtcgtgaag
    39661 tgaacttgaa catcaaacgt ttgatggaaa tcggttctta ccgtggaatc cgtcatcgcc
    39721 gtggattgcc tgttcgtgga caaaacacga aaaacaatgc acgtactcgt aaaggcccag
    39781 ctcgcgctat cgctggcaag aaaaaataat tcttaagtga aggaggttaa aacttcatgg
    39841 cagcaaaaaa agtgaatcgt aaacgccgtg tgaagaagaa tatagaagca ggtattgcac
    39901 atatccattc tacattcaat aacactatca tcatgatcac tgatactcac ggaaatgcat
    39961 tagcgtggtc atcagctggt tcattaggtt tcaaaggaag caaaaaatca actccttttg
    40021 ccgctcaaat ggcagcagaa actgctacaa aagcagcaat ggaacacgga ttaaagactg
    40081 ttgaagtaac agttaaagga cctggttctg gacgtgaagc agcaattcgt tcattacaag
    40141 ctacaggttt agaagtgact gcaattcgtg acgtgactcc agttcctcat aatggtgccc
    40201 gccctccaaa acgccgtcgt gtttaatgag tgccgctcat tttgagtttt atcagtaacg
    40261 tcattgaaca agacactttt cgttttgaaa ggggtaaaga taagaatgat tgagttcgaa
    40321 aaaccaagaa tcgcaaaaat tgatgaagaa aaagattatg gcaagttcat cgttgaacct
    40381 cttgaaagag ggtatgggac tactttaggg aattccctac gtcgtatttt attatcttct
    40441 ttgccaggtg ctgccatcac taatattcaa atcgatggtg tactgcatga attctctacc
    40501 atcaaaggtg ttagagaaga cgtcactcaa atcatcttga acattaaagg cttagcgctg
    40561 aaaatgtatg gccaagaaga aaaaaccctt gaaatcgata ttaccggacc tgctacagta
    40621 actgctggcg atattatcgt tgatagtgat gtagaaattc ttaacaaaga tatgtacatc
    40681 tgtagtgtct ctgaaggagc tacgttccat gctcgcttaa cagtgaaacc tggacgtggt
    40741 tatgttcaag cagatgaaaa taaaaaagaa gatatgccaa ttggcgttct tccagtcgat
    40801 tcaatctaca cacctgttcg tcgtgtgaac tatcaagttg agaacacacg tgttggacac
    40861 cgtgaagatt ttgataaatt gacgatggag atttggacag atggttcaat tgaaccgttg
    40921 gaagcaatga gtttgtctgc gaaaatcatg actgaacatt tggatatttt tgtgaactta
    40981 actgatgaag caaaaaatgc tgaaatcatg gtcgaaaaag aagaaacaca aaaagaaaaa
    41041 atgttggaaa tgacgatcga agagttagac ttgtctgttc gctcttacaa ttgtttgaaa
    41101 cgtgcaggaa tcaatactgt acaagaactt acaaataaat ctgagcctga aatgatcaaa
    41161 gtccgtaact taggacgtaa atcattagaa gaagttaaag caaaattgca cgacttaggt
    41221 ttaggtttac gtaaagaaga ttaaaacctt ttacgaagga gggaaaccaa cgtgagttat
    41281 cgtaaactag gacgcacatc tagccaacgt aaagcgatgt tgcgtgattt aacaactgat
    41341 ttattaatca acgaacgtat tgtgactact gaagctcgtg cgaaagaagt tcgctcaact
    41401 gcagaaaaaa tgatcacttt aggtaaacgc ggagatttgc atgcacgtcg tcaagctgcg
    41461 gctttcgtac gtaatgaagt tgccagcgtg cgtgaagaaa atgaggacat cgtcattgaa
    41521 tcagctttac aaaaactgtt caacgatatt gcacctcgtt acgcagaacg tcaaggcgga
    41581 tacacacgca ttttgaagac agaaccaaga cgcggtgatg gtgcaccaat ggttgtctta
    41641 gaacttgtct aaaatcacac taataacatc actttataga tgattctttt agagtgttat
    41701 gatgttggaa gatatttttc cgagtctagc tcagcgctgc tcctcaagcg aatatcgctt
    41761 ggggagtaag cactcatcat aaagtgtttt tttatacctt caattacctg tcatgacaga
    41821 tatgtttgaa ggcataaaaa aacattttaa gcgagaaata attgaaaatt ttgtcttttt
    41881 ttgatagtgt tagtaggaac tttaggatga agaaggagta atcatggagc caattattga
    41941 attagaaaag ataaattata aataccagcc agatgatctt cgccccgcat tgaaagatgt
    42001 ctcctttaca attgataaag gtgagtggat cgctattatt ggacacaatg gttcaggaaa
    42061 atcgacactt gccaaaacga tcaatggatt gcttctgcca gaatcaggga tcgtgaaagt
    42121 gggaaatcaa atattggatg aagaaaatat ttggacgatc cgacagatgg ttggaatgat
    42181 ttttcaaaat ccagacaatc agtttgttgg ttcgacagta gaagatgacg ttgcttttgg
    42241 tcttgaaaat caagggattc ccagagaaga aatgctagtg cgtgtgaaag atgcacttga
    42301 gaaagttaga atggctgagt ttgcttcacg cgagcctgct cgtttatcag gagggcaaaa
    42361 acaacgtgtt gccattgccg gcgtggttgc cttacgacct gatatcatta ttttagatga
    42421 agcaaccagt atgctagatc cagaaggtcg tgaagaagtg atctctacga tcaagaagat
    42481 caaagaagaa agtcaattga ccgttatatc tatcactcat gatattgatg aagcagctaa
    42541 tgccaatcgg atactagtga tgagacaagg cgaacttgtc cgtgaaggta caccaaaaga
    42601 aattttttct gctggtcctg aactgatcga cttaggtcta gatctaccat ttcctgaaaa
    42661 actgaagagc gcactgaaag aacgtggggt tgatgtgccc agtgaatata tgacagaaga
    42721 aaggatggtg gattggttat ggacatccgt tttgaacaag tagacttcac ttatcagccg
    42781 aatacaccat ttgaacaaag agccttattt gacatcaata tgacgatcaa agaaaacagt
    42841 tatactgcat tagtagggca taccggaagc gggaaatcaa ccttacttca gcatttgaat
    42901 gcgcttgtta agccaacaag cggtacagtc catattggag aacgagatat tcagccggat
    42961 acggataata agaatttaaa gcccatccga aaaaaagttg gcatcgtctt tcagtttcca
    43021 gaagcacagc tattcgaaga aacggtagca aaagacattg ctttcggtcc taaaaacttt
    43081 ggtgtcagcg aagaagaagc actagtccta gcaaaagaaa cattagaaca agttgggctg
    43141 gatgaaagct atttggaacg ttcgccattt gaactttcag gagggcaaat gcgtagagta
    43201 gcaatcgctg gtgtgcttgc catgaggccg gaagtacttg tattggatga accaacggca
    43261 ggacttgatc cccagggcag aaaagaaatg atggagatgt tttggcgtct tcataaggaa
    43321 cagcagatca ctattgtatt agttacgcat ttaatggatg atgtagccaa ttttgcagat
    43381 tatgtctatg tgttggaaaa gggacgaatc gttaatagcg gggagccgca agaggtcttt
    43441 caaaacattg aatggctaaa agaaaaacaa ctaggcgtac ctactgccac cgagtttgca
    43501 gaagaattaa tggctaaagg gatgaacttt gctaccttgc ctttgacagc tgaagaatta
    43561 gcagacgcta tcgtccatta tgctggagga aatgcccatg atgaataaat tgatatttgg
    43621 tcgttatatg cctggagatt ccttgatcca tcgattggat ccccgagcta aattattagc
    43681 cagtttttat tttatcggaa tcattttttt agcaaataat tgggaaacgt accttttgtt
    43741 ggcatttttc acattacttt ctgtcttctt gtcaaaagta gatttgaaat tttttatccg
    43801 aggcattcgc ccattgatct ggctaatcct cttcactgta gctttgcagg tcttgtttac
    43861 aagtggcgga gaaacttatt ggagttgggg tgttttccat atcactgaat ttggtgtgat
    43921 gaacggcctc tttattttct gtcgattcgt gttgatcatt tttatgtcga ctctactcac
    43981 gcttactaca ccgcctttgg aattatcgga tgcgattgag tacttgcttc gtccattgaa
    44041 agccgtacgg ttcccagtcc acgaaatttc attgatgctg tcgattgctc ttcgttttgt
    44101 gcctacattg atggacgaaa cagaaaagat catgaacgct cagcgcgctc gcggtgttga
    44161 ttttggtgaa ggaaacctag ttcagaaaat gaaagcagtc attcctttgc tgattccttt
    44221 atttgtcagc agcttcaacc gtgcggaaga tcttgcgact gctatggaag caagaggcta
    44281 caaaggtggg gaaggaagaa cgaaatatcg tgtattgcat tggcataata gagacacgat
    44341 ggttatcatt gcttttgtat tattaactgt gctactgatc tttttaagag gataaacgtt
    44401 cagtgggccg ctatctattc ttttcttgct gatctgtttg caagaaagga tgcaagcagt
    44461 gtatgacaag aaatattagg gtctgtggca gactaaggtc acaggcctct ttcttttatc
    44521 tgaaaagaaa gaacgaaaaa attttataga aaaatcatta ttccagataa ccttttagga
    44581 acaaaggaga gaaaaacatg gtacgttaca aagcaatcat tgcgtacgac ggaacaaatt
    44641 ttaatggatt ccaaaaacag ccaaatgggc gtactgttca agaagaagta gaaaaaacat
    44701 tacaaaaaat ggcaaatgga aaagaaatca cggtctttgg ttcgggaagg acagatgccg
    44761 gagtacatgc gatcgggcaa gtaatccatt ttgattatcc agaggagcgt ccgttagaac
    44821 gaatgcgttt cgcgctagat acccagtcac cagaggatat tgctgtaaga caagtagaaa
    44881 ttgtttcaga agacttccac gcaagatatt tggtcaaaga aaagacgtat caatttcgag
    44941 tggatatagg gaaaccacgc agtccttttc gacgccatta tgcaagttat tttccttacc
    45001 cacttgattt ggaaaaaatt cagcgggcgc ttcctgattt attagggact cacgatttca
    45061 cctctttttg tgcatcagga agctcgatag aggataaaat acgtacgatc tacgaggcta
    45121 agatggaggt aaatgaaaca aaagacgaat tgttgtttac atttagggga aatggcttct
    45181 tgtataagat gatacggatc ttagtaggaa cgcttttgaa aattggcaat ggccgattgc
    45241 ctgaagatag tatcccagat attattgcga aaaaagatcg taatgcagca ggacctacag
    45301 cacaccctga aggactttat ttgtatgaag tgaaatatga ataagaggtt atgatagaag
    45361 tggtcagctt caagaaataa gaaggtattt acgaaaatta cttctcaaaa ttttgcaaaa
    45421 tttcggctta ttttcgaaga agctacttct ggaacaccgt ttaagaaaga ggtcgtgagc
    45481 ctgtcattta actctgagtc acaaagagca atttcaaaaa acagcttctc atctttgatg
    45541 aatatcgtga cttgtggacg aggcgttgat aagtgaatca tctttttgtt acctattttt
    45601 tctgtaacca aaaaacgatg gaacgttgtt ttgattgccc ttggtgcgga cagtctgaac
    45661 aggttccatc gtttctttat tttctacgtc ttcttctgtg tgtgtttcgt ggttgttctt
    45721 ctattgctgg atcaatcgtg ccataaaagg ggcgattgcg gtcaaggtac cagacataaa
    45781 gaataaacaa tgcaatacat aggacaaaaa ggattgcacc tatcgtaaat ccttcaggca
    45841 agtaactgaa agtgacagta tgtttacctg ctggaacttt tatcatcaag aatgcgtctt
    45901 tgaaattgct gaccttgact ggtttcccat caattttagc tttccatcct ttgtcataag
    45961 gaatagttgt caccaacatg gaatcttcag cggtatttac agtcccagaa gcttttctgc
    46021 cagtggcttc catttcgaca cctttttctt tgatctgatt cattgcggct tgataggcta
    46081 caacatctag accaacgact ttaggctcca tgaaacttat ttctttcgtc ccataaaaac
    46141 ttgcagtaaa tgtcactgtt gtctcttgag gataatagcc aagatcatag tattgaccag
    46201 aaatgttgat ttgcgttttt cgcgatgtac cattgacagt gatcgtcgca ctagaacttt
    46261 ctaattgcgc aaaattggtt gggaacaaac ttaagtaagc ttgtgtattt gcaggcacat
    46321 ggacttccca agtgagctct tttggctggt tatctgctgt ttctgagtaa gtgacgccag
    46381 tcgttgtctt tgtgatcgtc gtattcaact gattcttcaa agtaggtgta tagaatgtaa
    46441 aatactgttc atttagacca gacaatgtat tgatcaagtt tgtctgactg gtcaaattat
    46501 ctgtcactgg ctgctcaaca tcattaacgg acaacggtgc agtaaatgct agtggcaatg
    46561 cattgacatt ctgatacagt gtatagtcgc cgctttgacc agcttggcgg aagccatatt
    46621 tactgaacgt gctttcgttt ttagaaatgt tatatttgat tcctgtaaac ccatccatca
    46681 gcaaagtatt attgttataa cggatattca aatttgtccc tcttgaacgg aaacctaatt
    46741 tatccaggta agaggaagaa ttacgatttc ttatagaaga aaacaaactg ataccactgt
    46801 aaccatagtt gatcgaatcg tttgcagata ctggatccag attttccaag cggtaaaacg
    46861 agtcggaatc tttttttgtt tcatttacaa gagctttcaa gtcaggataa ggatcagtat
    46921 ataaactgcg ggaagcataa ttccaatcat caagaatgcc atggatcata ctgttcgtat
    46981 tgaccagtgc ttcagcagac atgatcaata ataacaatat actcagatga ttgaatgaaa
    47041 gttttttatg ctgataaaat cccacagcaa aagcataaat ccctaagaaa accactgtca
    47101 atacaaagga agtcatcgtc acataggtgt agcttccttc aggtttgatg ccccatgcca
    47161 ccgcaaagag agcagctacg accaaaatga ttcctaacaa cagcccacca tcgtcttgtt
    47221 ctatcttttc ccatcctttt cctgcaagca ggagcactaa gaaagagaac agataggcat
    47281 aacggaataa aaacatattc ggtgcatgca taccatgcca gaacaaattc aatggaacaa
    47341 gataaaaact tgcgataagt atggcgaaaa gactgccgta caaaaattta tttttcaacg
    47401 gtactttccg actggcaaaa taaaacagac agaggactaa tggtagcaga cctgcgtaaa
    47461 cgaacggaat cgatccgtat tttgtcgtgt cataaacacc aatcatattt ttcatgatta
    47521 gatccagata agaagtcgct tctgttttca acgtagtgat ctgactcagt gcctcgccat
    47581 ttgaccgaag atccagcact gctggcaata caatgatcat tgaagcgccg cctgccagta
    47641 aagaagtgat accataagga acaatcgttt ttttatattc tgaccagttt gtcaatagac
    47701 gggcgataaa atataagaaa gaaaatacgc caatcataaa acccatataa aaactggata
    47761 aaaacaagag taaataactg ataaataaaa gtttcggccg tttaagatcc ataacacgat
    47821 gaatccccaa aatcactaat ggaagataaa caaatgcatc cagccacatg atgatctccg
    47881 aatgtgcagt gataaatgag atcaatgcgt aactgacgct aagtgcaaca tgcccccagc
    47941 gaggaatctt gaacgtacgt gcaagaaacc aaaaagccaa tccggcacag ccgaccttaa
    48001 gcaaagtcag aacatacagt gcatctggca tcatctggtt tggaaaaaag aacaccagtg
    48061 gcgtgaagat cccgcctaga tagtaagaaa ttaaagataa ataattcaat cctagagaag
    48121 cattccatgt ataaaaaata ctttgctttc caagaagaac atttcggaag ctggcatgaa
    48181 aattagaaaa ctgtgaaaag gcatcacttg ccaaaatact tctgctgctt ccaggataga
    48241 ttccgatact tagataaaca agtgctaaaa tcaaaaacgg aatcaggaaa ctagctgctg
    48301 cgtagcgtcc gttttctcta aagaatgact ttaaattttt tttcatattt attttccacc
    48361 aaatttctat gaagttgtat tcagtttatc atatccttgt ttttctctgc ttaaaaagag
    48421 tctagttttc acaaaaaaat aaaatttaga ataaataact tcataatttg ttcgtataac
    48481 aaaaataaac gcaaaaaaag ggtaggaatc aaaagtccca cccttaaaaa tttttattaa
    48541 ataaaaaacc gttcactatc tgaacggtcc aagtatcatt caattaaacg cgttcaactt
    48601 ttcctgattt caaagcacga gttgacaccc aaactttttt aggttttccg tcgaccataa
    48661 cgcgaacttt ttgtaagtta ggtttaacag tacgttttgt tgagttcata gcgtgtgaac
    48721 ggttgtttcc actttttgtc ttgcgacctg taaagtaaca aacttttgcc atggtaattt
    48781 cctcctttgc cttgatttgg agcaatagaa taattcagct cataccagac taacatatca
    48841 tatagaatgg gaatttgcaa gtacattttc atagatacaa ttgacaattg agaagaataa
    48901 ttgtaaatta aacaaggtta tacctgaaat tttgcaaggt ttttgagtac tttgggtaac
    48961 tgccttttat ttcaagttgc gctatgataa aatatgatag aagaaaaatg ggagtttttt
    49021 acccgttaag gaggctcatt catggctgta aaaattcaaa cgtcagctgg aaccattgaa
    49081 attaccaatg atgtaattgc aacagttgtt ggtggcgccg cgacagatgt gttcggtgtt
    49141 gtaggaatgg ccagcaaaaa tcaaattaaa gataatatta ctgagatttt gcgtaaagaa
    49201 aattactcaa gaggagtagt tgtccgccaa gaagataatg gtattgctgt agatgtttat
    49261 acgattatca gctatggtac gaaaatctct gaagtttccc gcaacgtcca agaaaaagtg
    49321 aaatacaatc tagaggcatt attaggcgta actgccaatt cagttaatgt ctttgtacaa
    49381 ggtgttcgtg tactgccaga ctaggcagga gactggctgg taactcagca gtcgatctga
    49441 aggaggatta tctaggtgag tgtaacagaa ataagtgcaa gccagttcca ggaaatggtc
    49501 caggcaggtg cgaatcgtct gcaagtaaat gcagaatacg tcaattcatt aaatgtattc
    49561 cctgtgccag atggcgatac gggaacaaat atgaatctat ctatgactag tggagctaag
    49621 gcagtagtag attcaacctc agaaaaagta ggagaattag cagcaatgct ttcaaaagga
    49681 ttgctgatgg gtgcgcgcgg aaattccggc gttattttgt ctcaattgtt tcgtggattt
    49741 tctaagcaaa ttccagatgt tgtctcatta aatgcttctg atcttgctgc agccttcact
    49801 catggagttg aaactgcgta taaagcagta atgaaaccag tagaaggaac gattttgaca
    49861 gtagctagag aagcagcgaa agctggagaa aagaaagcaa aaagtactga tgatgtcgtt
    49921 gaagtaatga cagctgttgt aaaaggagca aagcgtgctt tagctaaaac tccagacttg
    49981 cttccagtcc taaaagaagt cggtgtcgta gacagtggag ggcaaggact gctgtttgtt
    50041 tatgagggct ttttaagtgc attgaatgga gaattccaag cggatgaaac atatgaacct
    50101 tctccagcag aaatggatga tatggtcaat gcagaacatc atcgcagtgt ccaaggtcaa
    50161 atggcgacag aagatatcca ttttggttac tgtacagaaa tcatggtaaa aatcggagaa
    50221 ggacctacag tcgatagtac attcgattac gaagaattcc gtaactattt agatggtata
    50281 ggagattctt tacttgtggt caatgatgac gagatcatca aagtccacgt gcatacagaa
    50341 aacccaggag aagtcttgaa ttatggacaa aaattcggtt cattagtcaa aatcaaagtc
    50401 gacaacatgc gcttgcagca tgagacgatt ttggaacacg atgaagaagt gaacgagttt
    50461 gcgaaaaaag aaataccacg tatcccgtat ggtatcgtag cgatcgctgc tggtaaaggc
    50521 gtccaagagc tgtttgaaag tttaggcgct aattatgtta tcagtggtgg acaaacaatg
    50581 aatccaagta cggaagacat tgttaaagcg attcaagaag taaacgcaga taaagtgatt
    50641 gtcttgccaa acaataagaa catcttcatg gcagctgatc aagcagcaga agtagcagat
    50701 atcccagtag cagttgttcc ttcaagaacg atttcccaag gattgacagc tatgctttca
    50761 ttcaatgatc aagcttcttt agaagaaaac aaagaaacga tgacagaaat gttgtctagc
    50821 gttgtgagcg gtcaagtcac tcacgcgatc agagatacct caattgacgg tgtagagatc
    50881 aaagaaggag actttttggg catgatcgat ggaaaaatcc tgatctctaa cccagatatc
    50941 acagcaacag cgttaagtac actagaacaa atgatcaatg aggacacaga gatcgtaacg
    51001 atcttggcag gtgatgaagg aacagttgaa caagcaaatg aatttgctga gaaactaaca
    51061 gaaaaacatc ctgatttgga aatcgagatc caccaaggag atcagcctgt ctatccttat
    51121 ctattttctg ctgaataatt tctgtgtcag aatatctttc taacacagta ttcattcata
    51181 aaaggccttc ggttagtgat caggtatgat cgctgaccaa gtcctttttt tgttaaagga
    51241 gggaggaaaa atttatgcgt tctttagatg atccaatcac tatgctggca ggagtcggag
    51301 aaaaaagagc agctagtctt gccagcttgg gcatccaaac gattgaagac ttactgactt
    51361 attatccttt tcgctacgaa gatattcagg aaagaaatct aaacgagatc caggatcaag
    51421 aaaaggttac tttgaaagga atcgtagtct cgcctcctgt gatgaatcga tttggttata
    51481 aaaaaagccg attgcaattt cgtatgatgc aagaacatga tgtgttcaac gtttcttttt
    51541 ttaatcaacc ttatctaaaa gaaaaagtca ttttatcgga ggaaatcgcc gtatacggta
    51601 aatgggatgc aaagcgtaaa gccttgaacg ggatgaagat tttaggttct caaacaatgg
    51661 atgacttttc gccgatatat cacgtcaaca aatcaattcg tcaaacgaca ttagttgatc
    51721 taatcaaaaa agggtttcaa gaatacggca cattgattga agaaaatctg ccaaacaacc
    51781 ttgtggagaa atatcgactg cttgaccgag cttctgctgt aagaagtatg cattttccaa
    51841 aagaccacga agaaaaccat caagccaaac gacgtgtcgt ttttgaagag ttctttttat
    51901 ttcaaatgcg tcttcaagga ttgaaaaaag cagaaaaagc agaaacgaat ggtttggaga
    51961 tcctttatga tgtccaaaaa ttgaaacaat ttactcaaaa gctacctttt gaactgacaa
    52021 atgcacaaaa acgtgtaacg aatgaaatct gtcgggatct tcgcagtccg cagcatatgc
    52081 agcgactttt gcagggagat gtaggtagcg gaaaaacagt ggttgccgcg attgcgttat
    52141 acgctaccgt tacagcaggt ttccaaggtg cgttgatggt gcctacagaa atcttggcac
    52201 aacaacatat ggaaagcttg caacagctgt ttgatcccaa tgaagtaacc atcgcattga
    52261 ttacggggtc aacgaaagca aaagaacgac gtgagctcct tgaaaaacta gagcaagggg
    52321 aaattaacgt cgtgatcggc acacatgcat tgatccaaga tggagtcgaa tttcagaatt
    52381 taggtctagt tattacggat gagcagcacc gttttggcgt gaaccagcgg aaagtacttc
    52441 gtgaaaaagg ctggcgacca gatgtcttat ttatgacagc gactccgatt cctagaacat
    52501 tagctattac agcatatggt gagatggatg tatctatcat tgatgaactt ccagctggac
    52561 gtatcccgat cgaaacacgc tgggtaagaa cacctcaatt ggacagtgta ctggattgga
    52621 cgaaaaaaga actggcaaga ggacatcaaa tgtatgtcat ctgtccgctg attgaagaat
    52681 cagaagcatt agatgtaaaa aatgcagtgg aaatctatga aaaacttcgt gaactatttg
    52741 caccaaaata tgaagttggc ttgttacatg gtaaaatgaa gaatcaggaa aaagaagtaa
    52801 tcatggaagc cttcaaagac aaccagatgc agatattagt ttcgactact gtaattgaag
    52861 taggggtaaa cgtgccgaac gcgaccgtca tgctgatcat ggatgcggat cgatttggat
    52921 tggcacagct gcaccaactt cgtggacgtg ttggacgcgg aagtgatgct tcctactgta
    52981 tcttagttgc taatcctaaa aatgaactag gtgtggaacg gatgaaaatc atgacagaga
    53041 ccaccaatgg ttttgtacta agcgaaaaag atctggaatt acgtggtcct ggtgaagtat
    53101 ttggcttccg tcaatctggt ctgcctcaat ttgcgattgc tgacttggtg acggatggaa
    53161 atgttttgga ggtagccaga gaagaggctg cagccctttg gcaaataaag gattggcaga
    53221 tgcttcctga gtatcagcct ttagcgcaaa gcctccaaat agaaaatcag gataatcgtt
    53281 atttcgatta gcagactgtg ctatacttta tactgatgac ggaaaaagga gtggaaacaa
    53341 tgaaaattgc cgtagatgca atgggcggag atcacgcacc tcaagcaatt gtagaaggcg
    53401 tggctttagc gcagcaagat tttccagaag tagaattctt actttacgga aaagaagcag
    53461 aaatcaagaa atacttaaca aatgaaaaaa acatcacgat tatccataca gacgaaaaaa
    53521 tcaatagtga tgatgagcca gtaaaagcaa ttcgacgaaa aaagactgca tcaatggtcc
    53581 ttgctgcaca agcagtgaaa aacggtgaag cagatgctgt attttcagca gggaatacag
    53641 gcgcgctact agcagcaggg ttgttcatcg taggacgtat caaaaatatc gaacgccctg
    53701 gcttgatgtc taccttgcca gtagtaggga aagaaggtgc cggtttcgat atgctggatc
    53761 ttggcgctaa cgcagaaaac aaaccagaac atttgttgca atacggtatt ttaggttctt
    53821 tttatgcacg taaagtccga ggtatcggac gtcctcgtgt cgcactctta aacaacggaa
    53881 cagaagcaac aaaaggtagt gaagtgacga aaaaagccta tgaactcctt cagaatgaaa
    53941 caagcttgaa ttttattgga aacgtagaag ctcgtgagct actcaatggt gtggctgatg
    54001 tagtggtcac agatggattt acaggcaacg ctgttttgaa atctattgaa ggaacagcaa
    54061 tgaatatgat gaatctatta aaatctgcta ttctaaatga agggatcaaa ggaaaaatgg
    54121 gcgctatgct tttaaaagac ggcttacgtt ctttgaaagc agaaatggat tattccaaac
    54181 atggcggtgc cgttttgttt ggcttgaaag caccagtcat taaaactcat ggagcaacag
    54241 gccccgatgc tgttcgctat acgatccgcc aaatccacac aatgcttgag acagatgtag
    54301 ttggacaatt agtcgaacaa tttgaaaaaa gcgaagaata gtatttggat atctgaagcg
    54361 atccgttcca ttgaaaaaaa ggtggacatt cctgaaaaat gccagtaaaa ttagccatgg
    54421 tgtaggtaat tgacaagtgt atgattcttg gaggtgaaaa agttgactcg cgaagaagta
    54481 tttaacaaag tagcgaaaat tatttcaaat cattttgaga tagataccga taaagtgacg
    54541 gatgaattga acatcaaaga tgatttaaaa gctgattcaa ttagtattat ggaattcgtt
    54601 ttagagcttg aagatgaatt cggtacggaa atttcagatg aagacgctga acaaattgaa
    54661 acagtcggtg gcgcagtaga ttatatcagc agccatctta aataatttca aataaaagca
    54721 aagaaggata aaaagctgca tgcagctttt tatccttctt tttttaatct ttttgacacg
    54781 taaaagtcat ttttctgata attctggcga taaacagaaa ttatgttgca aagtaaagcg
    54841 gttttctata taattattaa taattcatta cacaaagata agaataacct ttgttataaa
    54901 acgatttatt ctgtgtaatg tcctttttta ttaagaaaaa cttccattta ggaagcagag
    54961 aggggtattc gattgtccga taaccaatta ttagaagttc agaacctaca tacaggattc
    55021 cgactcaaag atgactatta cgatgcagta gatgacgttt catttacttt agacaaaaac
    55081 gagattttag caatcgttgg ggaatctggg tgcgggaaaa gtaccttggc tactacgatt
    55141 atgggattac ttgatcctaa taacacaaaa attacaggtg agattttata taacgatctg
    55201 aatttaatcg acctgaacga aacgctctat aataaaatcc gcggaaatga tatcggaatg
    55261 attttccaag atccgttgtc tgcattaaat cctctcatga gaatcgaaga ccagatcaaa
    55321 gaaggacttt cctatcacac aaagatgaat gcagaacagc gtcaagctcg tgcactagaa
    55381 ttgctagaac aagtagggat tcctaatcct gcgagagttg gacgccaata tccccatgaa
    55441 ctatcaggcg gtatgagaca acgtgtcatc atcgcaatcg caatcgcctg caaacctcca
    55501 atcgtcattg cagatgaacc gacgacagcc ctagatgtaa ccatccaagc acagattctt
    55561 gatttactga aagaccttca ggaagaaaca aaaacaggga tcatcttgat cactcatgac
    55621 ttaggagtcg tagcagaaat ggctgatcga gtggcagtga tgtatggcgg acagttcgtt
    55681 gaagtagcag gtgtaaaaga actgtttgat catcctaagc atccttatac acgctcttta
    55741 ctgaactcta taccacaaga aggaaatcat gatgcagaac tccatgtgat cgaaggtgtc
    55801 gttccttctc tgaaaaacat gcctcgtaaa ggttgtcggt ttgcaccacg tattccatgg
    55861 ataccagcaa gcgatcatga agaagagcct atccttcatg aagtcgcacc gaaccacttg
    55921 gtacgttgct cctgctataa gcatttccac tttagagacg aaaaaggaga ggtgtagatg
    55981 acagaattaa tcacaatcaa agacctgaaa gttcattacc ctattcgcag tggattcttc
    56041 aatcgagtaa cggatcatgt atatgctgta gatggtgtgg attttattat cgaaaaagga
    56101 aagacatatg gtttagtggg agagtctggc tctggaaaat ctactacggg aaaagccgtt
    56161 gtgggattag aaaaggtaac agccggacaa atcatgtatg aaggaaaaga tgtaacgaag
    56221 cgaagcaacc gcaaaaaaat gggttataac aaggatgtgc agatgatctt ccaagattca
    56281 atgtccagtt tgaatcctaa aaagcgagtg ttggatatca ttgctgaacc catccgaaat
    56341 tttgaacggt taagcgatca agaagaaaag aaaaaggtaa aaggactgct agatatcgtt
    56401 gggatgccgg aagatgcgct atataaatat cctcatgaat tttcaggcgg acaacgtcaa
    56461 cgtctaggag tagcccgtgc tgttgctacc aatccaaaac tgatcgtagc cgatgaacct
    56521 gtatctgctc tggatctatc tgttcaagcg caagtattga atttcatgaa acgaatccag
    56581 caagaatacg gcctcagcta tttattcatt tcacatgatt taggtgtagt aaaacacatg
    56641 tgcgacaata tcgcaattat gtacaaaggt cgatttgtag aaatcggtac aagagaagac
    56701 atttataatg acccgcgaca tatctatacg aaacgtctgc tttcagctat tccgcaaatc
    56761 gatgtagaaa atagggaaag ccacaaagaa aatcgacgaa gagtagaaca agagtatatc
    56821 caaaaccaaa aagaatacta tgatgcgact ggtcgagtct acgatttgcg tacgatcaca
    56881 actacacaca aagtggcgtt gaaagatgga ggtgcaagct aatgtggaaa acgattctgc
    56941 gacgtgtttt attgatgatc ccgcaagtta ttattttaag tgttctgatc ttcttattag
    57001 cccaagcgat gccgggggat ccatttaccg gtttgatcaa tccaaatcag gatccggcag
    57061 tgatcgaaca gatgcgacaa gcagctggct tgaatgaccc atggtatgtc cagtacttcc
    57121 gttggatcgg aaatgctttg caaggcgatt ttggtcaaag tttcttatac aaacgctctg
    57181 ttgctgactt gatcggcgaa cggatcatca acactttata tctatcgatc ttgacagttg
    57241 tccttactta tttgattgct gtgcctctgg ggatgcttgc tggccggtat caaaattcaa
    57301 tcttggataa gattgtcgta atctacaact tctttagttt tgctgttcct ttatttatct
    57361 tcggcttgat catgctattt gtttttggtt atcgtttagg ctggttccca acaagtggat
    57421 cacaaacact aggtttgcaa ggtggattta tcactcaatg gatagaccgt ttgaaatata
    57481 ttctgttacc atcaattacc caagcatttt tggcgactgc cgtcacaatc caatatctgc
    57541 gaagtgaagt aatcgattcg aaatcgctcg attttgtcag aacagcccgt tccaaagggg
    57601 taccgacgaa taaagtattc agtcgacata ttttccgaaa tgcagcgttg ccgatggctt
    57661 ctcaaatggg gtatgaaatc acttcgttga ttgctggttc tgtagttatc gaaaagattt
    57721 ttggttatcc gggagtcgga aaactattta ttgattccat cggccaacgg gactataccg
    57781 tgatcacatc cttagtattg attttaggaa tcgcgacgct agtggggaca ttactttcag
    57841 atatcatcat gagtatcgtt gacccgcgta ttcggattca ataaaaccaa tcagaaagga
    57901 gaatggatcg atgagtgaaa aaaacaatac agcagttcaa gaaagtattc ctcctatggg
    57961 gatacgaatg attgctcgag aatttaaaaa agataaaatt gctatttttt cattaggttt
    58021 gctagtggtt ttattattag tgatctttat cggtgcgatg ctgaccgatc aagacaaagt
    58081 gatgtatgtt agtatcttcg acaaatacgc tgcaccagga acagtttcga cacaaggaca
    58141 aaagttcatt ctgggagcag atgaaggcgg acgtgatgta ttaggacaat tgatcatagg
    58201 tgctagaaac tctgttacga tcggatttgc gatcacgatc cttacttcga tcatcggggt
    58261 tggattaggg atcatctctg gctactacgg agggatcatt gacaacatct tgatgcggat
    58321 cgttgatttt atcatgattc taccgatcat gctgatcatt atcgtcttcg tatcgattat
    58381 ttccagatac aatgtctggt cctttatatt catcatgagt gcattttatt gggtagctaa
    58441 agcccgatta ttccgaagca agacattatc tgaagcaaga agagattatg tcagtgcctc
    58501 aaaaacaatg ggaaccaatg attttaaaat catgttccgc gaaatcatgc cgaatctaag
    58561 ttcattaatc attactaatt tgacgatgaa ctttgcggca aacatcggta tcgagacaac
    58621 tttgactttc ttaggtttcg gcttgccggc aaatgtacct agtttaggta cattgatcgg
    58681 ttatgcaagt aacggagaag tcttagtgaa tcggcaatgg atctggttac ccgcatcaat
    58741 ccttatttta gttttgatgt tgagtatcaa ttatgttgga caagcattca agcgttctgc
    58801 agatgcgcgc caacggttag gctagtatag aagcgaaggg ggaaatagga atgaagaaaa
    58861 cgatttttgg atttgcagcg ctgatgtctg ctgtcgttct tgcagcatgc gggggcggag
    58921 gtacagatac ctctggaaac aactctggag gcaacggcaa taacaaagag gaattaactt
    58981 ttccaattgc aacaaaaaat aatgatgatg caatcgaaga cggcgaatta gaagctgcgg
    59041 tcgcaacgga cagtcagttt aaaggattgt tccagtggga gttttaccaa gattcatatg
    59101 atgcagcttt tatggctcct tctcatgaac cgctatttgc aaacgatgac gatttcagaa
    59161 tcacagacga cggagctgca acgcttgatc tagatgaaga tacgaaaaaa gcgacaatca
    59221 aagtaaaaga caacgtcaaa tggtcagatg gtgaagatgt aacggcggac gatgtgattt
    59281 tcccttacga aatcattggg aataaagatt acactggtat ccgttatgac gacactttcc
    59341 gtaacatcgt aggtatggaa gaatatcatg atggaagtgc cgacacaatc tccggaatca
    59401 aaaaaataga cgacaaaaca gttgaaatcg aatataaaga agtaaaccca agcatgcttc
    59461 aatcgggtgg tggtatctgg tcatatgcag caccaaaaca tacgttagaa ggtgtagcaa
    59521 tcaaagatat ggaatcaagc gatcaagtac gtaaaaatcc agtgactttt ggcccttatt
    59581 acatgagtaa tatcgttgca ggagaatcag tagaatactt gccaaacgag cactactgga
    59641 atggtacgcc taaattgaaa aagatcacga tgaaatctgt accgatggca agtgcaactg
    59701 aagcattgaa ctctaaatta tatgatatga ttttccaaat gccaacagat acttacgata
    59761 cctataaaga aattgctggc tatacaaact taggccgtga acaaacttct tatacttatc
    59821 taggattcaa gttaggtaaa tggaatgcag atacaaagag tgtggaatac aatccagatt
    59881 caaaaatggc tgataaatca ttacgtcaag caatgggcta tgcattagat aataatgcag
    59941 taggtgaacg tttctatgca ggtttgagaa gcaatgcgac ttcattgatt cctccagtat
    60001 tccagtcata ccatgatgat gagatgaaag gattcacaca agatctagac aaagcgaaaa
    60061 aactattaga tgatgctggc tacaaagatg tggatgacga tggtttaaga gaagacaaag
    60121 atggaaataa actaacaatc aactttgctt cgatgtccgg tggggaaaca gcgcaaccat
    60181 tggcagatta ctatatccaa caatggaaag aaattggttt ggatgtccaa tacacaacag
    60241 gtcgtctgat cgaattcaat tctttctatg ataaattaga aaacgatgat cctgaaattg
    60301 atgtttacca agctgcttgg aatacaggaa ctgatccaaa tccaagtgga ctatggggac
    60361 caaatgctgc gttcaactac actcgttatg aatcagaaga aaatacaaaa ttgatcaacg
    60421 atatcagttc aagcaaagca tttgatgaag agtaccaaaa agaagcatat caaaaatggc
    60481 aacaatatgc atttgaagaa gcatttgcga ttccaacgtt gttccgtaat gaagtattgc
    60541 cagtcaacga ccgtgtcaaa gactgggatt ggtcttatga tgtaccaaac ccatgggcaa
    60601 atgtttctgt tacagcagat tcacgttcat aatcactaaa aatgaccgaa aaagggcgat
    60661 gtcctttttc ggttttttga tatttttctg tttatttctc taatattcag gtcaaggcgc
    60721 tgatttcatg acttctccaa taaacggtgt tcaaagtcca gtttctcggt attaagtcaa
    60781 atttatgcaa aaatatggaa agccattttc acaaaatttt ttcttcatac tcgaaactaa
    60841 acgaattttt cacaacttct cgtcaatcac taggattatt aaagctttac ttaccttttg
    60901 accgaaccat ttcaaatttg acaaaaaacc agtataataa ggaagtatcg aaaaaatatc
    60961 cataaaagga atgtggaaaa tggacaacca gctgacaaaa gaactaaaag aaaactataa
    61021 catcgttttt cacgatctaa atttgcttga acaagctttt acccattcat cctatgtgaa
    61081 tgagcatcgc aatctgcaat tatcggataa tgagcgactt gaatttttag gagatgccgt
    61141 tttagaactg atggtttctc agtatttatt ccgtttatat cctcatttgc cagaaggaaa
    61201 attgacaaaa acacgagctg cgatcgttcg cgaagacagc ttgtcgaaat ttgccaaaga
    61261 atgtcacttt gatcaatatg ttttgttggg caagggagaa gagaattcag gaggacgtac
    61321 gcgaccagca ttgctttgcg acctattcga agctttttta ggcgcgttat atcttgacca
    61381 gggattcgac acgacccatg cattcatcga aaaagtgatt tttcctaaag tcaaagcagg
    61441 tgctttttca catgagatgg atcataaaac aaaactacaa gaagtcttgc aaaaatcagg
    61501 tgatgtatca atcgaatacc gattgatcaa tgaagaaggt ccagctcatg aacgagtatt
    61561 ttggatcgaa gtttatgtag atgatcaatt gattggcact ggacaaggga aatcgaaaaa
    61621 attagcagaa caagctgcgg cggaaaatgc attggcagca ctgtaaaagt aagtgaggga
    61681 aagctttgta tttgaaacga attgaaatag ccggttttaa atcatttgca gacaagacag
    61741 tcattgattt tgaaaatagc gtcacagcag tggttggacc aaatggcagc ggaaaaagca
    61801 atattacaga ggctatccgc tgggtattag gagaacaatc tgctaaaagt ctgcgaggcg
    61861 gaaaaatgcc ggatatcatc tttgccggtt ccgatacccg taagcagctg aatattgcag
    61921 aagtcacagt gattttagac aatagtgatc attatctgcc attggagtac aatgagatca
    61981 gtgtaacccg ccgttatcga cggactgggg aaagcgagtt tttcatcaat aaacaaagct
    62041 gcagattaaa agatatccaa gaattgttct tagattctgg attaggaaaa gaatcgttct
    62101 caatcatttc tcaaggaaaa gtagaagcga ttttcagtag taaaccggaa gaccgtcgtg
    62161 ggatctttga agaagcagct ggtgtcttga aatacaagca gcggaaaaag aaagcagaac
    62221 agaagctttt tgaaacagaa gacaacctca gccgcgtcca agacatcatc cacgaattgg
    62281 aagaacagtt aactcctttg gccgcccaga gtgaggcagc aaaggagttt ttacggctaa
    62341 aagaaacact gactcaaacc gatgtctctt tgatggttgc tgaaatcaag acagcaaaaa
    62401 aagattggga caacaaacaa gcacagcttg cgaaattcaa tctagaatta ggcaaactat
    62461 ctgagtcgat ccaagaacaa gaatccattt tggcgaagca gcgtaaagaa aatgcgcaag
    62521 ccgatcgtct gatcgaaaaa aatcagcaag tattattaga tctttcggaa aaactgaagc
    62581 agacagaagg acaaaaagat gttttgcagg agcggacaaa gcatacacaa aaaagctcgc
    62641 aagaatatca aacttcatta gcagaagcac aaaaaaaggt taaacatttt gaaaagcttc
    62701 aagaaagctt gatgaaagca gcagcagaaa aagaaacaga gatccaaaaa gctgaagcaa
    62761 atctcataaa aacacagcaa gaattagaaa aataccaaaa atcaacgaaa gaattattgg
    62821 cagaattaag agatcaatat gttgatttga tgcaagaaca agcagctgta ggaaacgaac
    62881 tgaagtatct agaacgtcaa tatatacaag aaacagcaaa aagcaagcag acgttagcca
    62941 agcaatcgga agtagaagca tcagtagacc gattgatctt acaaaaagaa gaattgacac
    63001 aaaagcaggc gcagctaaaa tcatctttaa ctgaaacaaa agagaagtta gaaatgatcc
    63061 aacaaaacgg gaaaaagttc caagaagcat tagcaaaaga acagcctaaa atgtatcaat
    63121 taatgaatca agtccagcag ctacgtgctc gtaagaaaag ccttcaagaa atccaagaaa
    63181 actattttgg tttttaccaa ggggttcgat tggtcttgca gcataaacag cagctttcgg
    63241 gtattgtcgg agcagtggct gaactgatcg atgttcctgc agatttcacg ttagctatcg
    63301 aaacagcttt aggtggggca gcccagcatg tcatcgttga aaatgaaaaa gatgctcggc
    63361 aagccatcac gtatttgaaa cagcaaagag gaggaagagc aacattcctt ccactgacga
    63421 caatcaaacc aagacaatta ccggctcata tattgacaca agcagcagct gtagaagggt
    63481 ttatagggat tgccagtgaa caagtcagtt atcccgatca aatccaaact gtggttcaaa
    63541 acctgctagg aacgatcctt ttagccaaag acttaactag tgccaatgca atcgcgcaaa
    63601 cgatccgcta tcaatatcga gtggtttcct tagaaggtga tgtgatgaat gccggaggat
    63661 ccatgacagg tggagcgaat aaacgaggaa accaaggcag tttgtttgta caaaatcaag
    63721 aattgaaaca gttgactagt gaatttgaag aagcagataa acaactccaa gcacaagaaa
    63781 aaaaagttca ggagctgcaa caagaaacag ctcgtttagc cgaagaacaa gaagttttgc
    63841 gtacacgagg cgaacaatta cgttttgaag aacaagaagc gacaaatcag ctacaaaaca
    63901 tcataaatga acttgaacgt tttgaaaaag aaaagcaaat ctcaactttt gagacacgtg
    63961 aactgcagca gtttatcgaa gattacgaaa agcaaacgaa cgaattgaaa gacaaacaaa
    64021 cagatcttga atcgcaacga caaaaaatcg acgaagaaat caaatcgtta agtcaagaaa
    64081 gcgaccagat ggaagccaga agagcacaag tacaaagtca aaaagcacaa gaacaagcag
    64141 atcttgctgt cttgaaagag cagtttaatc atctgcagat ccagctccgc ggtgcccgag
    64201 tccaaaaagc agaggcaacc gaacgtcaag aagcgataga aaagcagttg gcgacattga
    64261 cagcagactt ttctgatcat gaagtcacag aagaaagttt ggaaaaacaa attaacgaat
    64321 tatctgctca gcgagaaaca ttaaaagccg agttggcaaa agccaaagag cagcgagata
    64381 gaaggcaaaa ggagatcgat aagcttgaag cagtcttggc agagcgtaat cgtgagcaaa
    64441 aagcacgact gtcggaacaa tccaaattag aagtccaaaa agaccgagct gaaatgattt
    64501 tagataatca tttgacctat ctgcagtcag aatatcagct gacatttgag aaagcttccc
    64561 aagactatca agaaacaacc gatatcgaag acagtagaac aaaggtttca tccttaaaag
    64621 aacagattga aaaactggga ccagtaaact taaattcaat cgaacaatac gaacaagtca
    64681 gtgaaagaca tacattctta gcaacacagc gagatgatct gttagcagcg aaaaaccaat
    64741 tgtttgaaac gatggatgag atggatgatg aagtgcggac aagattcaaa gaagtatttg
    64801 aagctatccg tcaagaattc aaaattgtct ttccaaatat gtttggcggc ggtaaagctg
    64861 aattagtact gacggaacca actgatttgc tgaaaacagg tattgagatt gaagtacaac
    64921 ctcctggcaa aaaactgcaa agtctaagtt tgctttctgg aggagaacga gcacttacag
    64981 ccatcgcgtt gctcttttcc attatccgtg tctgtcctgt tccgttttgt atcttagatg
    65041 aggtagaagc agcgttagat gaagccaacg tgaaacgttt tggtcgttac ttgagcgatt
    65101 tccaagatga cacacaattt atcgtagtca cacaccgcaa aggaacgatg gtcgcagcag
    65161 atgtactgta cggagtgacg atgcaggaat ccggtgtatc aaaaatcgtt tctgtacgga
    65221 tggaagatat caatgaagag gcgaaaatca acgaaacgaa agtgtaggaa cgaaaatgat
    65281 caaattagta gcaatcgatt tggacggtac attattaaat gaacaaaagc tgatttcaga
    65341 tgagaataaa caagcattag cacaagcaaa acagcaaggg gtcaagatcg ttctatgtac
    65401 aggacgcccc ttagctgcga tggcgcatta tctgcaagaa ttaggactgg tagatgaagg
    65461 tgactacagc atcactttca acggcggttt ggtacaaaaa aatgatactg gtgaaatcat
    65521 ggaaaagaaa gtgatggaag tatcagatat ccaacgctta tacaaactgg ctcagcaatt
    65581 ggatctgcca ctagatgttt tgtctgacaa tgtggtttta cagctgccta gtgccccaag
    65641 gaagcaatcc ttgtacaatg tgttgaataa gctgctgcaa ttccaaccag cggatataag
    65701 cgatattaca gatgaatttg tgcttaataa ggcagtaatc gcttatgacc aagaagaatt
    65761 agatcctaaa atcaaagaaa taccagcaga attccatgaa atgtatgaga tcatcaaaac
    65821 aagaagtatg cttttggaat ttatgccaaa aggtgttaca aaagcttatg gtatttcttt
    65881 attggcaaaa gatcttggtt tgaaacaaga agaaatcatg gcgatcggtg atgaggaaaa
    65941 tgatttaccg atgatccaat atgctggact aggcgtagcg atggagaatg ctgtgccttt
    66001 cgtcaaagaa gcagcagatt tcgtgacagc cagcaatgaa gaaaatggcg tagccaaagt
    66061 gatccaaaaa tttgtattag agtaggggga gtcaaatggg attttttgat aaaatcaagc
    66121 gtgcttttac tggcgaagag aaaacagaag aagttgaagt acaggagaag tacgacaagg
    66181 gtttagaaaa gacgcgtaaa acatttggtg aacggatgaa tgagttgttt gccaattttc
    66241 gaacagtcga tgaagacttt tttgaagaat tagaagaaac acttatcggt gcagatgtag
    66301 gttttgaaac agcgatacga attaccgaag cattacgtca agaagtgaaa ctcagaaatg
    66361 cgaaaaaatc ggcagaagtc caaaatgcga tcatcgagaa acttgtggat ctctatgaag
    66421 cggaaggaat caatgaagta aatgaactaa atatccaaga agacggacta acagttattt
    66481 tgtttgtcgg tgttaatggt gtagggaaaa caacaagtat tggtaaactt gcccatcagt
    66541 tcaaaaacga aggtaaaaaa gtattgatgg cagcagcaga taccttccgc gctggagcaa
    66601 tcgaccagtt agttgtctgg ggcgaacgcg ctggtgttga agttgttcgt ggcaatgcag
    66661 gaggcgatcc agcagctgtc gtattcgatg ctttggaaag agcaaagaca gagcaggcag
    66721 atgttttact agttgatact gcaggccgac tgcaaaataa agtgaacttg atgaacgaat
    66781 tagaaaaaat aaaacgagta atccagcggg aattaccaga agcaccccaa gaaacacttc
    66841 ttgtattaga tgcaaccact ggtcaaaacg cgatggttca agcaaaacaa ttcaaagaaa
    66901 caacagatgt gaccggtctt gttttgacaa aattagatgg tacagcaaag ggcggtatcg
    66961 tacttgctat ccgtaatgaa ttgcatttac cagtgaaatt ggtcggtctt ggtgaaggaa
    67021 tcgatgactt ggagccgttt gatccaaatg attttgtcgt tggattgttc aaaggattac
    67081 taaaagaaga ataacccatg tctaaaaagc aggagctgtg acaaaagttg cagctccttg
    67141 tttgaatgat tgtttttaac atagctgtgt ttttgatgcc ttcattttta tacttatttg
    67201 taagcggttc tcaaacaatt tcgttctttt tcttagactt tttgattata attagattca
    67261 tatgaattgg aggtcactta atgatgaatt ttaagcaatt attattgcat gtcaatgcgc
    67321 gcccttttat cgaccaagct cgatttggta tagaacggga aggacagcgg gttgatcttg
    67381 caggaaatct agcaaaaacc gatcatccag caatctttgg cgatcgatcc tatcacccct
    67441 atatccaaac agattttagc gaaacacaaa cagagatgat cacccctgtt accgattcta
    67501 ttcccgaatt atttcagtat ctcgctgctg tttatgatgt gactgctcgt tctataccga
    67561 aagaagagat gatctggcca ttaagtatgc cacctgcctt accggaaaaa gacgaagaga
    67621 tcattattgc aaaattaaaa aatttcgaag atgtcttgta tcgacgttat ttagcaaaag
    67681 aatacgggaa gcgaaaacag atggtaagcg gtattcattt caattttgaa tttggcgatg
    67741 agttgctaag aacattgttc agccatcagg aagaatttca agatttttcc gaattcaaaa
    67801 cagaacttta tttgaaaaca gcgagaaact ttatgcgcta tcgctggatg atcacttatt
    67861 tattcggtgc ttcaccaatg agtgagaaga actacttttt agatgaatca catccgcaag
    67921 aacctgttcg cagtattcgc aacagtgcgc tgggctatac gaatcatcca aatgtgaaag
    67981 tttcctatgc ttctatgaaa cagtatttag cagatatcga gcgaatgatc gaagaaggga
    68041 aactttcgga ggagaaagaa ttttatacac cgcttcgttt ccgaggcgga aagaaagtcg
    68101 cagatttagc aacaacaggt gttcgttata ttgaattgcg gaatatcgat ttgaatcctt
    68161 atgcaagatt gggtatcaat ccagagcaag ttcggttctt acaattattc ctgatgtaca
    68221 tgttatggac agaagaaaaa gaagactgtg accaatgggt agcagaaggt acgactcgaa
    68281 ataacaaagt agcactagaa caaccatcag atcaaacaga atttcatcaa gaaggtagag
    68341 aaattcttga aggaatgaag caaatgctgg ttgaattgga ttggttggat tctctttatt
    68401 tagtagagga agcattgact caaatggatc atcctgaaca aacgttagca gcgaaactct
    68461 atcaagaagc acagctatcg agtcagcaag aagttgctgt ggcattggga catcaatatt
    68521 ataaagaaag ccatgaacgt ccctatcagt tggccggttt tcgcgaaatg gaactttcta
    68581 ctcagatatt tatgttcgat gccatccaaa aaggtgtcca agtcaaagta ttagatgagt
    68641 cggatcaatt tttgcgtctg caattccaag accatgtaga atatgtgaaa aatgcaaaca
    68701 tgacaagtaa ggacagctat atcgtcccac ttatcatgga aaacaaaaca gtaacgaaaa
    68761 aagtcttaaa agaagctggg ttccgggtac caggaggcgc agaattttca tccatggaag
    68821 aagcggtaaa agcttatccg agatttgccg agcaggcatt tgtgatcaaa cctaaatcaa
    68881 ccaattacgg gttaggtatc acgattttca aggaaggagc tagtctggaa gattatcaag
    68941 caggtttagc cattgctttt cgtgaagaca gttcggtttt agtagaagaa tttatgccgg
    69001 gaacggaata tcgtttcttt gtgatcgacg gagaagtcca agccatcatg ttgcgagtcc
    69061 ctgctaatgt tattggtgac agtatccgaa cagtgaaaga acttgttgaa gaaaaaaata
    69121 gtgacccttt gcgtggaacg aatcatcgtg cacctttaga attgatccaa ttaggtgaat
    69181 tagaacaatt gatgttgaaa gaacaagggt tgacgatcga atcagttccc caagccaatc
    69241 aaattgttta tctaagggag aactcaaata tcagtacagg tggtgattcg atcgatatga
    69301 ctgatgaatt ttctgaagct tacaaaaaaa tcgccgtttc tgcggtagaa gcactaggag
    69361 ctaagattag tggtatcgat ttgattatac cagataaaga aatcgatcct acaactgata
    69421 aaaaagcata tgggatcatc gaagcaaatt tcaatccagc catgcatatg catgtttatc
    69481 cttttgcagg aaaaggcaga agattgacga tgaatgtatt gaaattgtta tatcctgaag
    69541 tattttaaaa gaaaaagctt ttgacagatt gtcctatttg atgtcaatct gatcaaaagc
    69601 tttttttgca tgtaaaaatt tatgtgtttt ttcgttttaa aataaacgat cccagaagct
    69661 ttttttcttt tttggttttt ctttttcagc tggcgaagag ttttcttctg ggtatttttt
    69721 ttcgatatca atcacaggtt catttactgc ttcttttgct gttaagagca atccgaattc
    69781 ctcaggattt gcagtgacgt aatcgttgac tacagtgaaa cggctattgt ttttcgttgc
    69841 tagctggata tattggtttt ggacagaaag gggtagagcc ccattcaaga gtatggaagc
    69901 atctggatgt tttttgagtt cttttaaaaa gttttctttg ttttttggtt ggatcatttc
    69961 agcaacagtc atactaagat agcagcgttc cctgaatgtc cctaaatatt tatgctgttc
    70021 ttcagggttc actaaaggtg taccataaag tcccttatcc aaatggcttt ttacatcatc
    70081 ttgtgccata aaaaataccc tccttcttct ctttgtcttt cttacgatta tagcatattc
    70141 ccaataatac ctaaaacata tgaaaaagcg gttgcttttg ttcaacgatt cacttaaaat
    70201 aagaatgaaa atcattctca attagaaagg tggattggat gcgctacgga cgactagtag
    70261 cattattagt tattttaagt agtatttcat tgttcatcgg agtatcgaat gtacaattgt
    70321 cagaactgct ccagttagat gggcaaagct tacaaatctt gctagtgact cgtttgccac
    70381 gaacgatttg tttgatcctt gtgggagcga ccagtagtat ttgtgggttg atcatgcaac
    70441 atttgaccca aaataaattt gtttcaccaa cgactgcggg cacgatggat agtgcgcgtt
    70501 taggtatttt agtggctatg attttcctac ctggtgcatc cttgattgtc cgatccttga
    70561 cagcattttg ctttgctttt gcaggaacat tgcttttttt atcccttacg cgattgttcc
    70621 tccagaaaaa tcaattgatg cttccgttga ttggagtcat gttagggaat atcattggtt
    70681 cagttgctac tttttttgct tatcaattcc agttggtaca aaatatgact tcatggcttc
    70741 aagggaattt tgcgactgtg atacgaggaa attatgagtt attgtatgct gttgttccat
    70801 tgttgctctt ggtttggcta tttgcttacc gatttacgat cattggtctg ggcgaatcgt
    70861 ttgcagtaaa tctgggaata gattaccaga agatgcagtt tttaggcatt ggcttagtat
    70921 cattggctag tgcagtcatg ttgatcatgg taggaagcat tccttttatc ggagtgatca
    70981 ttccgaatct tgtctctaga atatatggag atcaagtgca caaaacaatt ggcgtaactg
    71041 ctttagcagg tagtttgttc ttagtatttt gcgatcttgt cgctcgcgtg ttgatttttc
    71101 cttatgaaat accagtcagt gtgatcgttg gaatcatcgg tggggctatc ttcctttatc
    71161 ttctgataag gggacgacga tatgcttaaa aaaaagccat ctatttttat tggtatgctt
    71221 gttcttgttt tatttgttac aggaatgtac ttgacgatcc atacatatgg aaactggcat
    71281 tttgcatgtg cattgaggag taagaaaatt tttgcattta tccttgtctc gctagctgtc
    71341 agcagtgcca caatcagctt tcagacaatt acgcataacc agtttttgac tccttcagta
    71401 ttgggacttg agtcgttata tgtccttgtc caaacggtcc ttttcttctt tatcggagga
    71461 gtgacgatgc ttcagcagac aggtacagtt tttttcttat tgaacgttgg gctgatggtc
    71521 gggctgagtc tattgttatt tttctttctt ttgaaaaaaa tgagagcgaa tcttttttta
    71581 ttactgatgt cagggatgat tttaggtacg ctttttggta gtgtcagtac ctttttacaa
    71641 gtcatcatgg atccgaatga atatgatttg cttcaaggca gattatttgc aagctttggc
    71701 aatatccaga gtcagtatct tttgcctgct tctttgatca ttatcggctg ctggtacctg
    71761 ttatttcgat atcgacatgt gctagacgtt ttccatctcg gaaatgaaca agcagtaaac
    71821 ctcgggatag acacagatcg ttttgaattt ttgattttat gttgtgtcag tcttttgaca
    71881 gggacatcaa cggctcttgt gggtccagtt acttttttag ggtttattgt cgcaaatatc
    71941 agttatcgtg tcttttctac ttatcgtcat ggtcagctat ttatcggaag ctttctggta
    72001 gctatgctgt ttctgacagg aggacagctc gcagtagagc agattttcgg ctggaataca
    72061 acagtgagtg tcgtcgttga atttgtcgga ggaatttatt ttgtcacaaa attgattcgc
    72121 gaaaggaaga gtatcctatg attgatttac aaaaggtcaa taaactgtac catggaaaat
    72181 cagtggttaa agatgtacag ttaacgattc ctgaaaagaa attgactgct tttatcggtc
    72241 caaatggtgc tggaaagtca acggtgttgt cgatgatcag tcgtttgata ccaaaagata
    72301 caggtgaaat ctatttagac cataacgaag taaaggcttg gaaatccaat gaattagcca
    72361 aaaaattatc gatcctacga caatcgaatg aaataaatgt ccaaatcacc gtgaaagaat
    72421 tagtagaatt tggacgattt ccttatacga aaggaaaatt gacgacaaaa gacgaggaaa
    72481 tcgttcagga atcactggaa caccttgggt tagaagaaat agcggatcaa tcgattgaca
    72541 ctctttcagg cggtcaactc caacgtgcgt atattgcaat ggtgctagcg caagacacgg
    72601 attatatttt actggatgaa cctttgaaca atctagatat gaattttgcg gttcaaatca
    72661 tgcagatttt aaaaaattta gtacaagaaa tcgggaaaac aattgttatt gttcttcatg
    72721 atatcaactt tgctgcaagt tatgcagacg aaattgttgc aatgaaagat ggacagttat
    72781 ttacgcaagg aacgacccaa gaaatgataa aaaaagatgt attggatgaa ttatacgaaa
    72841 tggacatacg gatttgtgag attgaaggcc gcagattctg tctttatttc taaaactaac
    72901 attaggagag aagagatgaa aaaattagta gtaggtatct tgattctagc atcatttggt
    72961 ttagcagcat gtggaaatag caacaacacc agtcaggcag acacaaaaaa aagcagcaca
    73021 caaactgaaa ctacattgac gataacagat agtaatggag accaaatcga ggtgccaaat
    73081 aatccagaaa aagtcgtcgt attcgacaat ggttctttag atacgatgga tgctcttggt
    73141 gttggagata aggtagttgg agcagctaca agcagccttc ctgagtatct ttcatcctat
    73201 aaaaaagtag aatcagcagg tggaataaaa gaaccagact tagaaaaaat taatcagctt
    73261 cagccagatc taatcatcat ttctggccga caaagagatt ttcaaaaaga tctaagtcaa
    73321 attgctccga ccattttcct ttctttagat gcgaaaaatc catgggaatc tttccagcaa
    73381 aatgtcactg ctttagggga gatctttggt aagcaagaag aagctaaaac tcaattagag
    73441 gaattatcta gtgcaatcga tcagacgaaa aagaaagcag aagctactga caaaaaagca
    73501 ctagtcacac ttgtgaatga agggcaatta tcagcctacg gatctggttc tagattcggt
    73561 ttcatccatg atttatttgg ctttgaacaa gcagatgatc aaatcgaagc atccactcac
    73621 ggtcaaagtg tttcttatga atatgtgctg gaaaaaaatc cggatatctt atttgtagta
    73681 gacagaacaa aagctattgg tggagacgac agcaaagatg atatttctgc caatgaactg
    73741 gtagcacaaa cgaatgctgg taaaaatcag cagatcatct cccttgaacc agatgtctgg
    73801 tatttaagcg ggggcggttt agaatcaatg aaactgatga tcgaagatgt gaatcaagca
    73861 tttaaatagc ataaataaca gaggattaaa ctctcatact atgtaacaat aatgtttctt
    73921 acattcttaa atagcaagag cctatcctca aagaagttat caaaaaccat ccgtaaaatc
    73981 gtattgatga ttttacggat ggtttttatt tatttgagaa atcttttttc aacgtgaatt
    74041 cattatctac atcatacggg ttgattctag aatggggagt ggcaaaaaat agtgtgatcc
    74101 attttttcag catcaagatc gcctcctttg atttgaatca aaggtacaaa gcgaaactta
    74161 gataaaactt aaatttagat atttttattt tgtaccgatc tcttctttaa acgatgttcc
    74221 atctgctgct tctcggcaat aagtccacta gactagaaaa tatgagaagc tattttctgt
    74281 ccagcgttct tattactcaa agcaaatcgc agttgtcaca atctcttcta aacgatgttc
    74341 catctgctgc ttctcggcaa taagcccact agactagaaa atatgagaag ctattttctg
    74401 tccagcgttc ttattactca aagcaaatcg cagttgtcac aatctcttct aaacaatgtt
    74461 caatcagctg accttcggta ttagctcaaa gcactgattt cacaacctct atttaggcat
    74521 gtgtctcagc aagcggttga tagttgatga tctgcatttt tcgataaaaa tcggcgggaa
    74581 gcttactcca ccgttttaat aattgataga aataactctc ttctgctttg attttatact
    74641 cgcttaatat caatccaaaa ctattcgttt tttttgaagc taatttttta taaatatcca
    74701 atgctttggt tttatcttca ttgacaaaga gtgtttttac ttcaatataa gaaccctcga
    74761 tattttgtgt ttcttcgata aaatagatgt tttttatata ttccttcata gtgattcctc
    74821 caaagaagtc ttttatttga taagtttact ctaccgaaga gttgttaata atccgtgaac
    74881 aaaaagtaat tctttttcca atagttgtta aaggtttttt attttttata gtattttttg
    74941 ttatttaaga aaggaataag gttaaacaaa taacaggttg ctttttgggt ctttttccgt
    75001 tcaagaaact gaacgaagaa aagggaaagt tgtctctttt gtgataagag aaatcataat
    75061 aaacgaatga cttactttcc aataaattga gaacagtcag aaccagacaa attagttgca
    75121 tctgatgaat aaagtgatta ttctatgaca tgaggtgata cagatgaaat tagaagtgac
    75181 aaaagaagca caagaagtat tgaagaataa attagaagca gacgatcaac ttttattaga
    75241 tatcgaaaac ggtgacggtc catttgcaga aagccaagtc agttgccaat tggatacggc
    75301 atttcgattg atcattgtca aaaaagatac acaaacagat ctaagtcttt attctgtagt
    75361 ggctgataca ccagttggac caatcaaaat caaagacagt gcaatacttt acatggatga
    75421 tccatccaca cttgctttag aaccaactta taatagttta caattaaaag gaccaagcgg
    75481 cttgagaaaa ggcaacttgc aagttgttcg aaaagactag agggataaaa tgggaaaatt
    75541 ggcgatcatc agcgacctac atgtggacat caataaattc ggcgaatcag agctgatgca
    75601 gctttggcaa gttttgcaaa agcacaagat cactcggctt catctagccg gagatacggc
    75661 taataaagta gaccgttgcc tagctgttgt cgaatttttc gtcaataaag gattatctac
    75721 gacatttcat ttcggcaatc acgagttggc agatgtgccc aatgaacaga tgatggacca
    75781 ttttccagac ccacattttt taaatgaacg gtatattgct ttaaatgagc agaccgttct
    75841 tttagggatg aatggctggt acgactatca gttttccgaa ttgaaagatt acgaggcaat
    75901 cgtacgaatg aagcgtttgt attggtatga tcggttcatc cagcgtggga aaacagatct
    75961 cgaggtcaat gcagaaatgc ttgcagctgc aaaaaatttg ctagaccagc tggaagcaaa
    76021 aaaacttgag gtgattttat ccactcattt cgttcctaaa cgggcattta tcatgtatca
    76081 atctgctcct tatgagcgtt ggaacaaatt aaatgcattt ttaggatcag aatcattcgg
    76141 agaattactt gaccgttatt caaatatcaa acaagtagtt tttggccata cgcatcgtcg
    76201 ctttgaagat aaatccatcg gtcacacgat ttacagctgc agaccttttg gttattatta
    76261 tgaatggcag ctgacccgag attttgtctt gaaagaacag ttgctagaca gctatgatcc
    76321 tatgaaatta cgtgtcctct tgcgtgccca tcacccagct tttcaggcct atcaaacaca
    76381 acatttgcaa gaggaatttg aacaagcgat gacaatcgtt ccgtattgag acagcattag
    76441 taatgtgtaa aagaaaatac cttgtaaaac ataaggataa gtaagagcag tagatccatt
    76501 tatcgtggat atgctgcttt ttttgcggaa aataatcagc gaatgtttat ttgatcggat
    76561 gatttgtaac tcgcagagta ctagatgcaa gcgtgtattc tttttttgaa cgataatcac
    76621 aaaaaaaaat a
//
LOCUS       DAJSAW010000005        72517 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJSAW010000005 DAJSAW010000000
VERSION     DAJSAW010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.005"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..791)
                     /locus_tag="ONT64_000401"
     CDS             complement(<1..791)
                     /locus_tag="ONT64_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR2301843.1"
                     /translation="MFPFFKKKPLKLQNTIILVVCFSTFVSLIVSAILIRNFILSMNR
                     SSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIMIASNLDFIVVMDPEGIR
                     YSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFRYFVPIYYDQEIIGAVCV
                     GLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMKKVLLGLEPQEIAEQLSE
                     KTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDFEVGK"
     gene            966..1490
                     /locus_tag="ONT64_000402"
     CDS             966..1490
                     /locus_tag="ONT64_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR2301844.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            1506..3005
                     /locus_tag="ONT64_000403"
     CDS             1506..3005
                     /locus_tag="ONT64_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCR2301845.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3093..3695)
                     /locus_tag="ONT64_000404"
     CDS             complement(3093..3695)
                     /locus_tag="ONT64_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR2301846.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            3799..4755
                     /locus_tag="ONT64_000405"
     CDS             3799..4755
                     /locus_tag="ONT64_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCR2301847.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(5060..6016)
                     /locus_tag="ONT64_000406"
     CDS             complement(5060..6016)
                     /locus_tag="ONT64_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2301848.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            6190..6750
                     /locus_tag="ONT64_000407"
     CDS             6190..6750
                     /locus_tag="ONT64_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301849.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            6743..7567
                     /locus_tag="ONT64_000408"
     CDS             6743..7567
                     /locus_tag="ONT64_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCR2301850.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            7577..8194
                     /locus_tag="ONT64_000409"
     CDS             7577..8194
                     /locus_tag="ONT64_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR2301851.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            8212..8895
                     /locus_tag="ONT64_000410"
     CDS             8212..8895
                     /locus_tag="ONT64_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR2301852.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            9578..10813
                     /locus_tag="ONT64_000411"
                     /pseudo
     CDS             9578..10813
                     /locus_tag="ONT64_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            10821..11708
                     /locus_tag="ONT64_000412"
     CDS             10821..11708
                     /locus_tag="ONT64_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2301853.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            11725..12561
                     /locus_tag="ONT64_000413"
     CDS             11725..12561
                     /locus_tag="ONT64_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2301854.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            12564..13187
                     /locus_tag="ONT64_000414"
     CDS             12564..13187
                     /locus_tag="ONT64_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301855.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            13184..14476
                     /locus_tag="ONT64_000415"
     CDS             13184..14476
                     /locus_tag="ONT64_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2301856.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      14835..14932
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            15048..15629
                     /locus_tag="ONT64_000416"
     CDS             15048..15629
                     /locus_tag="ONT64_000416"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR2301857.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            15632..16939
                     /locus_tag="ONT64_000417"
     CDS             15632..16939
                     /locus_tag="ONT64_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCR2301858.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            17232..17720
                     /gene="purE"
                     /locus_tag="ONT64_000418"
     CDS             17232..17720
                     /gene="purE"
                     /locus_tag="ONT64_000418"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR2301859.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            17713..18837
                     /gene="purK"
                     /locus_tag="ONT64_000419"
     CDS             17713..18837
                     /gene="purK"
                     /locus_tag="ONT64_000419"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR2301860.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            18850..20145
                     /gene="purB"
                     /locus_tag="ONT64_000420"
     CDS             18850..20145
                     /gene="purB"
                     /locus_tag="ONT64_000420"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR2301861.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(20325..20702)
                     /locus_tag="ONT64_000421"
     CDS             complement(20325..20702)
                     /locus_tag="ONT64_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301862.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            20958..21686
                     /locus_tag="ONT64_000422"
     CDS             20958..21686
                     /locus_tag="ONT64_000422"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR2301863.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            21686..21940
                     /gene="purS"
                     /locus_tag="ONT64_000423"
     CDS             21686..21940
                     /gene="purS"
                     /locus_tag="ONT64_000423"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCR2301864.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            21942..22613
                     /gene="purQ"
                     /locus_tag="ONT64_000424"
     CDS             21942..22613
                     /gene="purQ"
                     /locus_tag="ONT64_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HCR2301865.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            22614..24836
                     /gene="purL"
                     /locus_tag="ONT64_000425"
     CDS             22614..24836
                     /gene="purL"
                     /locus_tag="ONT64_000425"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCR2301866.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            24821..26260
                     /locus_tag="ONT64_000426"
     CDS             24821..26260
                     /locus_tag="ONT64_000426"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR2301867.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            26283..27335
                     /locus_tag="ONT64_000427"
     CDS             26283..27335
                     /locus_tag="ONT64_000427"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR2301868.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            27332..27910
                     /gene="purN"
                     /locus_tag="ONT64_000428"
     CDS             27332..27910
                     /gene="purN"
                     /locus_tag="ONT64_000428"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR2301869.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            27907..29442
                     /gene="purH"
                     /locus_tag="ONT64_000429"
     CDS             27907..29442
                     /gene="purH"
                     /locus_tag="ONT64_000429"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR2301870.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            29458..30732
                     /gene="purD"
                     /locus_tag="ONT64_000430"
     CDS             29458..30732
                     /gene="purD"
                     /locus_tag="ONT64_000430"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR2301871.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            30854..31768
                     /locus_tag="ONT64_000431"
     CDS             30854..31768
                     /locus_tag="ONT64_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR2301872.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(31901..33466)
                     /locus_tag="ONT64_000432"
     CDS             complement(31901..33466)
                     /locus_tag="ONT64_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR2301873.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(33459..34910)
                     /locus_tag="ONT64_000433"
     CDS             complement(33459..34910)
                     /locus_tag="ONT64_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2301874.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(34924..35940)
                     /locus_tag="ONT64_000434"
     CDS             complement(34924..35940)
                     /locus_tag="ONT64_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2301875.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            36160..37248
                     /locus_tag="ONT64_000435"
     CDS             36160..37248
                     /locus_tag="ONT64_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HCR2301876.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            37302..38828
                     /locus_tag="ONT64_000436"
     CDS             37302..38828
                     /locus_tag="ONT64_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR2301877.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            38830..40035
                     /locus_tag="ONT64_000437"
     CDS             38830..40035
                     /locus_tag="ONT64_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2301878.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            40292..41269
                     /gene="guaC"
                     /locus_tag="ONT64_000438"
     CDS             40292..41269
                     /gene="guaC"
                     /locus_tag="ONT64_000438"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR2301879.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            41533..42957
                     /locus_tag="ONT64_000439"
     CDS             41533..42957
                     /locus_tag="ONT64_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR2301880.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            43418..44527
                     /gene="pdhA"
                     /locus_tag="ONT64_000440"
     CDS             43418..44527
                     /gene="pdhA"
                     /locus_tag="ONT64_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCR2301881.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            44530..45507
                     /locus_tag="ONT64_000441"
     CDS             44530..45507
                     /locus_tag="ONT64_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCR2301882.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            45532..47175
                     /locus_tag="ONT64_000442"
     CDS             45532..47175
                     /locus_tag="ONT64_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR2301883.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            47183..48589
                     /gene="lpdA"
                     /locus_tag="ONT64_000443"
     CDS             47183..48589
                     /gene="lpdA"
                     /locus_tag="ONT64_000443"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR2301884.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            48751..49026
                     /locus_tag="ONT64_000444"
     CDS             48751..49026
                     /locus_tag="ONT64_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCR2301885.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            49032..49799
                     /locus_tag="ONT64_000445"
     CDS             49032..49799
                     /locus_tag="ONT64_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HCR2301886.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            49967..51796
                     /gene="typA"
                     /locus_tag="ONT64_000446"
     CDS             49967..51796
                     /gene="typA"
                     /locus_tag="ONT64_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCR2301887.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            52057..53829
                     /gene="recQ"
                     /locus_tag="ONT64_000447"
     CDS             52057..53829
                     /gene="recQ"
                     /locus_tag="ONT64_000447"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCR2301888.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            53943..54245
                     /locus_tag="ONT64_000448"
     CDS             53943..54245
                     /locus_tag="ONT64_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCR2301889.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            54248..55411
                     /locus_tag="ONT64_000449"
     CDS             54248..55411
                     /locus_tag="ONT64_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR2301890.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            55437..58865
                     /locus_tag="ONT64_000450"
     CDS             55437..58865
                     /locus_tag="ONT64_000450"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCR2301891.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            58886..60010
                     /locus_tag="ONT64_000451"
     CDS             58886..60010
                     /locus_tag="ONT64_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301892.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            60000..60437
                     /locus_tag="ONT64_000452"
     CDS             60000..60437
                     /locus_tag="ONT64_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR2301893.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            60421..60759
                     /locus_tag="ONT64_000453"
     CDS             60421..60759
                     /locus_tag="ONT64_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCR2301894.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            60878..61438
                     /gene="rsmD"
                     /locus_tag="ONT64_000454"
     CDS             60878..61438
                     /gene="rsmD"
                     /locus_tag="ONT64_000454"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCR2301895.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            61435..61926
                     /gene="coaD"
                     /locus_tag="ONT64_000455"
     CDS             61435..61926
                     /gene="coaD"
                     /locus_tag="ONT64_000455"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR2301896.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            61916..62959
                     /locus_tag="ONT64_000456"
     CDS             61916..62959
                     /locus_tag="ONT64_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HCR2301897.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            63118..63846
                     /locus_tag="ONT64_000457"
     CDS             63118..63846
                     /locus_tag="ONT64_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR2301898.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            63962..64207
                     /locus_tag="ONT64_000458"
     CDS             63962..64207
                     /locus_tag="ONT64_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR2301899.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            64204..64422
                     /locus_tag="ONT64_000459"
     CDS             64204..64422
                     /locus_tag="ONT64_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR2301900.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            64558..65097
                     /locus_tag="ONT64_000460"
     CDS             64558..65097
                     /locus_tag="ONT64_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCR2301901.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            65090..67363
                     /locus_tag="ONT64_000461"
     CDS             65090..67363
                     /locus_tag="ONT64_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCR2301902.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            67401..68447
                     /gene="holA"
                     /locus_tag="ONT64_000462"
     CDS             67401..68447
                     /gene="holA"
                     /locus_tag="ONT64_000462"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR2301903.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(68536..68790)
                     /locus_tag="ONT64_000463"
     CDS             complement(68536..68790)
                     /locus_tag="ONT64_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR2301904.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(69190..69459)
                     /locus_tag="ONT64_000464"
     CDS             complement(69190..69459)
                     /locus_tag="ONT64_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301905.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(69929..70384)
                     /locus_tag="ONT64_000465"
     CDS             complement(69929..70384)
                     /locus_tag="ONT64_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR2301906.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(70522..72450)
                     /locus_tag="ONT64_000466"
     CDS             complement(70522..72450)
                     /locus_tag="ONT64_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCR2301907.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        23605 a        12514 c        15818 g        20580 t
ORIGIN      
        1 ttttttccta cttcgaaatc caatagctgc tctgtttggt agaaaatgtt ttttgcaggt
       61 gtattgatca agaggatagt tttatcagaa gagatggcta agattccttc atctacttca
      121 ttttcgataa tcgttttttc acttaactgt tctgctattt cttgtggttc aagtccaagc
      181 agtacttttt tcattttttg ggcaagaaaa acggcgccta tgatgcctac gctaagtcca
      241 ataatcaatg aaaaaacaat cattttttgt ccatctttga cttgattgtc aatcgtgttg
      301 tttgttaagc caacacaaac tgcaccaata atctcttgat cataataaat agggacgaaa
      361 tagcggaagc cgaaccctaa cattccttct ttttcagaga aaaattcttg tcctccaatc
      421 acttttttca catcttcatg acttgagaaa ggaagaccga tgtaacttgg gtctgcgtga
      481 gaatatcgaa tgccttcagg gtccatgaca acgataaaat ctaaattcga tgcaatcatg
      541 atttcatttg ttattccttg aatcgcatgt gtcgggtcac cagtctgcag ctttttttgg
      601 atgagtttat cattggcaac gattttagca atgctagaga ttttatctga cacactagat
      661 cggttcatcg aaagaataaa attcctgatc aatatggcag aaacaatcaa agaaacgaat
      721 gtggagaaac aaacgacaag gatgatcgtg ttctggagct ttaatggttt tttcttaaaa
      781 aaaggaaaca taggctacct cgaaagaatt tattgtgaaa attttaacat gaaaacaaaa
      841 atttaacaaa ttgtcaacga taaacacaac cttcctataa aaaacaccac tgagtcggta
      901 aaagaagcta tacagaattt tcaaacattg gtatgataga cgtagaaatg gaaggaggcg
      961 tgtatatgat agggggaatc gcacttgaga cgatttattg gtatacattg ctgatttgtg
     1021 caggattggc agtcctattg attatttttg gagatgtatt cgattttgat ggcccgcttg
     1081 atccgatgct tattatccct tggttaactt tcacaagctt atttggtttt ctaggtgagc
     1141 gtttaacaga tcaatcatct ttatttttat ttattgctag tggaggcata tcgatagtgt
     1201 tggtattttt gcttaatttc tatatattga tgccgatgaa acatgccgaa tcaacgatgt
     1261 cgtcttctga aaaaactctg gaaggacaag tagctgttgt gatcacaccg atccctgtac
     1321 gaggtatggg agaaattcag ttgaaaagtg ttaccggttc cattagtcgt ccagcttgtt
     1381 tttacagccc ccaagaacag acagctgagc ggggaacgaa agtattgatc attgagatca
     1441 aggaaagaat ctgttatgtc atgccttatg aaagcatgat aaaagcttaa aggagcgtgt
     1501 gttaaatgga aataccagtt gtttttatcg cgatcgcaat cgcagctttg attttactga
     1561 tgttgctgat cgtctttgta gcgaagtacc agacagcaaa accggatgaa gcgttgatca
     1621 tcagtggaag ttatttagga agcaaaaatg tgcacattga tgaaagcaat aacaaaatca
     1681 aaattgtgcg tggaggtggc gcattcgtct tgccagtttt tcaacgttcg aatcggatca
     1741 gtctattatc gagtaaattg gacgtctcca cccctgaagt gtataccgaa caaggcgtac
     1801 cggtgatgtg tgatggtact tcgatcatca aaatcggttc atctgtagaa gagattgcta
     1861 cagcagcaga acagttttta ggcaagacaa gagaagaact ggaaaatgaa gcaagagaag
     1921 tattagaagg acacttgcgc tcgattcttg gttctatgac tgtagaagaa atctatcaaa
     1981 atcgcgataa gttcagtcaa agcgtacaag aagtcgcttc tgtagacctt gcaaaaatgg
     2041 ggttgatcat cgtttctttc actatcaaag aggtcagaga caaaaacggt tatcttgact
     2101 ctttagggaa accaagaatc gcccaagtca aaagagatgc agatatagca gaagcggaag
     2161 ctctaaaaga aacgcgtatc aaaaaagctc aagcagaaaa agaatcacaa gcagctgaat
     2221 tacagcgaca aacagagatc gcagaatcat taaaagaaaa agaactgaaa ttagcaacct
     2281 acaaacaaga acaagatgtt gctaaagcaa aagcagacca agcctacaat ttagaaagtg
     2341 cccgagcaca acaacaagta atcgaacagg aaatgcagat taaaatcgta gaacgccaaa
     2401 aacaaatcga actggaagaa aaagaaatca cacgtcgtga aaagcaatat gattcggaag
     2461 tgaagaaaaa agcagatgcc gatcggtacg ctaaagaaca agaagcacaa gctcaaaaag
     2521 tcaaagaagt gactgaagcc gaagcagaac gattccgtgt ggaagcactg gcagaagcgg
     2581 aagccaataa gacacgttta gctggacaag cagaagccga agcagcacta gcaaaaggaa
     2641 aagcagaagc cgaagcaaaa caaaagatcg ccaatgcctt caaggaatat ggcgaagcag
     2701 ctgtcttgag tatggttatc gatatgctac cgcaattgat gcgcgaagca gcacagccgc
     2761 taggaaatat cgaaaaaatc tctgttgtcg atacaggaag cagttcagga gaaaccagtg
     2821 gagcgaaccg agtgacaaat tatgccacaa atttgctttc ttctactcaa gaaacactaa
     2881 aagcgacaac aggacttgac ttgaaggaaa tcgtagagaa tttgtcgaca cgtgggacta
     2941 ctagccaagt aaatatgtat gaagcgccaa aagaggcaga acaaaactcg atgaaggaag
     3001 aataaagtag tacacttcac cgttcaatca aaaatggagg aagacttttt aaagttcttc
     3061 tctccatttt tttattttat tgcaaaagaa aattagtcct cgctattttt cttttgtttt
     3121 tggatcttag cttctatctc ttgtaaattt gttcttagca atcgttcttt cttttggagt
     3181 tcgaccaaat cttctgatag ttcttccttc ttttctgttt caggaggacg aagaaagaaa
     3241 ttgataccga ttgcaatgaa tgtccctata aaagtatcta gtacacgttg gatagcatac
     3301 aaagcggatt cgccttgagg gatgctcaac gcaatcagca acaacgtagc tattgcggaa
     3361 atgataccgg aattattgtt aataccatct gacacgacga tcacaataat aactagaata
     3421 ggaagcaaga tcaattctac caaaaaatca ttgtgaaaat agcttttgac aaaaaaataa
     3481 atgatagcta atgtaccacc taatgtattg ccgatgatcc tagactttcc aaaagaaacg
     3541 gttgtagtca agtcctgacg caaagaaaaa acagcagata atgcagcaat catcgggatt
     3601 cctcgttgag tcaaatgaaa aaacaagata cataaaaaaa cagctaaggc acttttgatc
     3661 gtacgcatac ctagtcgaaa acggccaaat tgcatagaga ttctcctttt tggtcttttt
     3721 actatcatac ctaaactttt gtctgatagc tagtttttca ttattcaagt ataattaaaa
     3781 taaaaaaggg ggaagtaaat gcgtatagga atgcgaacag tcaaaacagt gatcagtgtc
     3841 ttcttttcga tggtcacagc gggtagctta tcgctgttgt attggccagc tgcagggatc
     3901 attgcattat taagtgtagg aaataccaag cgctctacat tgcgtacggg catgaatcga
     3961 atcgcagcct ttatattagc tacggcaaca gcactgttca gtttttttgc tgttgggtat
     4021 actatttttg gcttcagtct ttttttattc ctgttcatcc cgcttgcctc tcgcttcaaa
     4081 ttaaccgaag ggatagtagt aaattcggtt ttggtcactc attatttagt ggaggaaaac
     4141 atgtcttggc agctgatcgg caatgaagcg ggattaatgg cgattggatt agtatttgct
     4201 ttgcttgcaa atgtttacat gccagatcgt acgaagcagc tgaaggaaaa ccaaatacag
     4261 atcgaaaaag aatttcgaaa tattttgaga caaatggcag aatttctttt atcagaaaat
     4321 aaagaagatg tgcagatcaa atgcgaacat ttactgacat tcattcgcga aagccaagaa
     4381 gatgccagag aacatcaaga aaactactgg ctcagacagc cgctatacta cgaaacttac
     4441 ttctctatga gaagagcaca ggccaatgtg attaaagaca tgctggaaaa tctagagaga
     4501 atccagcaac cagcttatta tggaaaacat atctatggtt tattgattta tactgcagaa
     4561 acattttcag agtcaaatga tggcagacaa attttaacac gtattgaaga agtttatgta
     4621 ttgtatcggc aaatgcctct gccgactagt cgccctgaat tcgaggatcg agcagaattg
     4681 ttccagtttt tgcagtcttt taaaagtttt attgaaatca aggcagaatt ctcccaacaa
     4741 aaaatccaaa aatagaggtt gtgaattcag ctaatattca ggtcaaaaag ctgatttcac
     4801 aacctcttct ttaaacggtg ttctcctgtc aactcctcgg tcacaagtca caatattcaa
     4861 caaacatgag aagctgtttt ttgaaattgc tccttgtgac tcggagctga acggtaggct
     4921 cacaacctct ttcttaaacg gtgttcaaaa gctttcctct gcggtaataa gtccagccaa
     4981 acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg cagaacagct
     5041 ttttcacaac cttttaaaat tacatatttt tccatagaac ttgcatttga tccaagattt
     5101 cgatgacatc ttttgtcaag aagagagagc gattgggcaa gtcaccttca atcatgtgta
     5161 ccatattctt tatttcgtaa ttcattgctt cagatgtatt cccgctttcg atgaattcct
     5221 ttgtaccatc tgtaaataag atctccgcac gatcagcacg cgggtaatca gcaatcgtca
     5281 cataaccgtt ctcaaaggca acgataccga cttttggcat ttttgcctga aaagtcaagc
     5341 tgacagcagc aagttcattt tctttgttac gcaagatagt gacggattgt tcgtccacgc
     5401 ctgtttcaaa aggaaccatt gtagaggcaa ctacttcagg ttgagagctc aaaaagaaac
     5461 gagcgaaaga tacagcatac gtaccgatat ccaacagagc tcctccagct aattctggat
     5521 tgaagaaccg gtttttaggg tctggttctt tgtaactgcc gaaaggtgct tggatcattt
     5581 ttaaagcccc cagttttcct gtatccatta ttgagcgtaa ctgctgataa agtggcatgt
     5641 tgaagattgt catcgcttct gctaagatca aattcttttc ttctgctaag cgcatagcgt
     5701 cagccagttc ttttttattc atcgtgatgg ctttttcaca tagaacgtgt ttgcctgctt
     5761 ccaatgctgc caagatatgg tcgatatgct gtctatttgg tacagcaatg tacacgatgt
     5821 cgatttcagc atcgtctaac atttcttgat agcttccgta agcttttgga atattgtaac
     5881 ggtgagcgaa gttttccgct ttttctgaag ttctggaagc aactgctgtt agttcgctcg
     5941 tctcttgatt gaagctttcg gcaaactcat gtgcgatggt accaagacca acgattcccc
     6001 attgatattt tttcatctga aaacgctcct tcatagttgg attgagtttc ttgtctgtct
     6061 attattataa caaaaatcac gaagaataaa gaggagagga cttgaaacat taaaaaaaat
     6121 gaaagcagcg atttaagagg tgctttttga tcaaaactta agtagaatag ttaaaaggag
     6181 gggataaacg tgcatcaagt atatattgat cttattatga gtgtattatt agaacagttt
     6241 gaaactgaaa aaaatttttt agatgaatac ttgctgatca gtccagaaca atggcaaaaa
     6301 tggaaagatg gcgagaaaaa tctgccatct gaagcgatgc agaaagtaaa aaatcttttc
     6361 actgattatg aatggatgct aacgcaaaag attttgcgtc aaacgattat ttttcccgaa
     6421 aaacgaaacg ttgccgttgc agagtacaag caaatcaaaa cacgcatcgc gcaaaaatgg
     6481 cttagcagca aagcagcaaa agtcgagctg atccctttga aaaataatca gcaggctggt
     6541 agataccttg acttgaaagt aagtattcaa tatgatgaat ggggctatga tgatattttg
     6601 aattttcgtc tgccagcaga tgttcaaaaa cagatcgaag gagaaaaaat cgaacttctc
     6661 gattgggtga atgaaaatct ggaagaaact tacgttcatg aggcgaatgg ggaacgtgaa
     6721 gaacaacgaa aggatttttc aagtgaataa atttttagga tttggagcag ttgtgattat
     6781 gatcgcagca atgggctatg ttatttttag cgatgatgat tcgcatgttc aagccggcaa
     6841 tcaagcagtc gaacgaacaa gcaccaaaac aactgctagt aaagaaaaga acagtcaaac
     6901 aaccagcagc aaggtaaagg acaaagaaac aaaagatcgt acgtccaaac aattgccaga
     6961 tgttaaaaca gacgattgga agctagtttt ggttggtcca gatcacaaat tagaaaaaga
     7021 aattgatgag tctactcaat tggcatcatt aagtaacgga tacttgatcg acaaacgcat
     7081 tgctccagaa tatgaagcat ttgccaaggc tgctgaagca gctggatttc cattagtgat
     7141 ggtctcagct taccggtctg tatcatccca acaacaagtt ttcgaacaaa atgtccaaga
     7201 tgtgatgagt cgtcaaggtg tatcagaaga ggaggcgaca aagattacta aagaaacgat
     7261 tacggagcca ggatacagcg aacaccacac aggtctggca gtcgatgtcg tcgatcaaaa
     7321 ctggtacaac agttatcctt ctactgtatt ggatgcaagt tacggagatc agcctggagc
     7381 taaatggatc gcagataatg ctgcgcagta tggatttatc gttcgttatc caaaaggaca
     7441 cgaagatata actaagatca catatgaacc ttggcactta cgttatgtcg gtaaagaaaa
     7501 cgctgagtat atcaccaaac accaattgac gctagaagaa tatctgaatg aattaaatga
     7561 aaagtaggaa ttgatcatgg cgaaaaaaaa gtggaaaaag caaaagcgca agaatatccg
     7621 ttggccagca gtggttgccg gtgtagcagt gatactctta gcctttgttt tttccttgaa
     7681 gaatctgtct tctcctttta ctgataatca gcaagaaaat gaagcgcaat cccatcagca
     7741 atttattgaa cggcttgttc cgcatgccca agaactacaa gatggttatg gagtgcttcc
     7801 tagtatcatt ttaggacaag ctatattaga atcaaattgg ggaaaaagta ccttggctag
     7861 caaatacaat aatcttttcg ggatcaaggc ttatggggat cagaagaaag taagcctgga
     7921 aacaaaggaa tttgttaacg aagaatggat aactatccaa ggagacttca aagtgtatga
     7981 ttcatgggaa cagtccatgg atgaccatac acaactgttc gtccaaggag tagattggaa
     8041 tcctgcgctg tatgaaaaag tgattacggc caccaactat caggaagccg cacaagcatt
     8101 gcaagatgca ggttatgcga cagaccccgg ttatgcgcaa aaaatcatcc aggtcatcga
     8161 gacgtatcaa ttgaatcagt atgaccggag atagcaaagg agaagtggct gatggcagaa
     8221 caatttacgc cagaaatcat gacggaattg agaaaagata tcgtaagagt accggaaatc
     8281 attttagaag caagcgggat caagatattt ggaaaattga tccgttcgat catctttact
     8341 acggatattg cgatcatacg taatacaaat gcgaatgcag taattgctgt gtatcctttt
     8401 acacctcatc cagcgattac taaaagcatt attgaggcag ctgacattcc agttttctcc
     8461 ggtgtaggtg gtggtttgac acaaggtgct cgttcttcct acatgagcat gttcgcagaa
     8521 gcgcaaggtt cgttaggggt cgttttgaac ggtcctacgc ctttagagac agtaaaagaa
     8581 gtttgtcgag tagttgatat tccagttatc agtacggtaa cgtcgaaata tacagaaata
     8641 gatgaaaagt tagcagctgg tatttcgatt atcaatatca gtgcaggtaa agaaactgca
     8701 gaagcagtac gccattttcg taaaaaatat cctgaacttc ctatcattgc gacaggtggt
     8761 ccaacggaag aaagtattgc tgagaccatt caagcaggag caaatgccat cacatatacg
     8821 ccaccaagta gcggagagct ttttagtcgt aaaatggaaa aatatcggga aactgagaaa
     8881 aagaatcatg aataacaggt gagaatcaaa agacgaggaa gagcagatag tggctcttcc
     8941 tcgtcttttg tactccagta ctatttgttc agaattcaaa aaaaagagaa atttttttgt
     9001 ttttttacct gtaatgtaca taagaagagt taaaaccttt tgttattatt agcattctaa
     9061 caaaaaaatg tcttgattta ttgaagcgct ttctatagaa tgaaaaacga ataaaaacta
     9121 aaaaaggcac ccgacaattt agtagcagct aaattgtcgg ggcttgaaaa gtcagcggga
     9181 actgaccaat caagttgcca tagtcaatgc ttgtgcatcg acgtgtatat tgtacctaat
     9241 ttcatacaga attactagtt atttttctgt tttttaggga agagcgcaca cgaagaagca
     9301 tgcttattta gctatgtcaa ggatattggg cagttgtctg atatcctttt ttctttgcgt
     9361 aattatcctc ccatcattga agcaagaagt gtttttagcg atgaaagaag aatcagcact
     9421 atctaatttt tcttgaaagt attgagttgt tcatcctagg cagaacgata aggcaagtct
     9481 tggtttttat tccccttgaa aaagatcgtt taataaatat gttctgtata aaaatacagt
     9541 atattgaaaa ataacaatgg aagtagaggt gcagagaatg aagaaaagaa tgagaatcat
     9601 cttagcttta agtgttatgt tattcttctt gacggcttgt gggcaagaag atgacaaaaa
     9661 aatcacgctg aacatggctt ggtggggttc gcaagtccga cacgatgcaa cagtaaaagt
     9721 aatcgagctt tatgaagaaa aaaatcccca tgtgacgatc gattatgaat tttatgatta
     9781 tgaaggatat tacacaaaat taggtacact agcagcatca gatgatatct gggacatttt
     9841 ccaactgaac aatcaatttc ctcaatacat caatcagatt gaaccattag atgaatatgt
     9901 ggagcaaggg ataattgata tttctaatgc agatgaatcc tatttagcaa cgacgactta
     9961 cgacgggcaa ttagttggac tgtctaacgg ggtgaattcg tttgcgattg cttacaataa
    10021 aaatatattt gaacaattaa atatccctga accagcttca aattggacat gggatgaatt
    10081 tgaacaaatc agctatcaaa tcactgaaga attaggaatc tatgctattt caagattcga
    10141 agattttata gctggctgta ttattcaaat accacaagtt gagaaaggat tgaattttta
    10201 taatcgggaa gatctatcta acagcttggg atttgaaaat ccagactatc taacagattt
    10261 tttccagctg cgtaaaaatc tggtagacaa aggggcccat ccagaaccag gtggtggagc
    10321 tgcaagtacg acggatctgt cttttcaggc ggttagggat agagaagcgg caatggtgtt
    10381 tctatcaagt aaccagttga cagaaatatt gtcaggggct cctgaagata tggagattca
    10441 gttgattaat ccacctcgca gaaaagcaga tggagaatct ggtatccctt tacgttcttc
    10501 gcaaatgctg agtatggcaa aaagctcagc ccataaagaa gaagctgcta agtttattga
    10561 tttctttcaa aacagtgaag aagccaatga aattttaaaa ggagaacgtg gtgtaccaat
    10621 catgtcgaat attcgagaac agctcgctcg tgcagatgat gcaactttgc aagcgacatt
    10681 tgattattta gaaatgattt ccgatatgga caacggcgaa gtgaatgtct ttgatagtgc
    10741 tttgaatccg gaaattcaag accaatacaa tcttttagtt gaaaaagtca tttatgaaga
    10801 agtaacagca gaaggaaggc atgaaaaaag aaaaactaaa acggttcttg gatcaagaca
    10861 atgtggtcgg ttacgtattt gcttctccat tcatcctagg atttctattg ataacagtga
    10921 taccgatgat cttgtcactt tattattcgt tcaccgatta tcgtttggga aatccagtct
    10981 catggatcgg cttggataat tatataaaga tgctgagtga tgcccgtttt ttgaactcca
    11041 tccgagtcac ttttctctat gtatttgcat ctgtacctgt gaaactgatc tttggtctat
    11101 tgattgctta tcttttaacg aggaatatca aaggaacgac tttctttcgt tctttgtatt
    11161 atgtgcctag tttgatcggt ggcagtattt ctatcgctct agtttggcgg gagttgtttt
    11221 ctaaaaacgg gttagtgaat attttcctgg aacgattagg cgcacctgcg ctttcctggt
    11281 ttagcgatca aaaactggtc atgatccctc ttgttttgat gtctgcttgg caattcggtt
    11341 cggcaatgat catctttgct gcaggtatca aagagatccc aggctcgtat tatgaagcgg
    11401 cagagatcga tggagcgaac aaatggcaga cgttttacaa gatcacgtta cctcttcttt
    11461 cgccgatcat tttgtataac ttggttatgc agacaatcgc ggcgtttatg acttttacgc
    11521 aggcatttat cattacccaa ggtggtccga atgatgccac gaacttttat gctctttatg
    11581 tgtataacca agctttttct tttaattcta tggggtatgc tagtgctatt tcgtggatca
    11641 tgctgttgat tatgggagct gtcacctttc tgattttcaa atcatcaaaa aaatgggtcg
    11701 ttgtgtgaat gagaggagga agaaatgaat aatacagtaa cgaaatggtt tcaatacggt
    11761 tttattttgc tattaggtct agtcatgatt tatccggtct tatggatgat cgcaggttct
    11821 tttaaaacgg atcaagagat tatgagtggt tcgctgaatt tgattccaga gacttttcaa
    11881 tgggaaaatt acgcaaaagg atgggcgggg tttgctggaa tttcattttt tactttcttt
    11941 aggaattcgt ttgtcatctc tgcagtttcg acgataggga cagttctgag ctcgacttgt
    12001 attgcttacg cattatcgag aatcgatttc aaaggaaaaa gattctggtt cgtcgtcatg
    12061 ttagtaacga tgatgattcc tgcacaagtg attttgattc cgcagtttat tatttacaat
    12121 cggctgaatt tggttgggac gtatgtgccg ctgattttac cacatttttt tggacaagca
    12181 tttttcattt accagatcat gcaattcatg gtcagcatcc ccaaagagtt agatgaatca
    12241 gcgattattg acggatgcag caagtatagt gtctttacca aaattatttt tcctttgctg
    12301 aaaccttcga tcatcacgac gatcatcatc cagttttatt ggaaatggga cgacttcatg
    12361 ggaccattga tttatttaaa caagccgaga tcttacacgg tatcgattgc catcaaatta
    12421 tttgcagacg ctggatctag tacgacttac gcgagtatgt ttgcgatgtc tacgttgtcg
    12481 ctgctaccgg tttttctgat tttcttattc ttcaataaat atcttgtcca ggggatcagt
    12541 acaagcggtc tgaagggctg aatatgaaaa agccaataga agagatgttt ctttacaagc
    12601 tcaactacta tgtctgtcgg atttttttaa ccagtcttat cagtaatgct cttttcgctt
    12661 tagttctagg atgtttgttc actttaggat tagtgctgtc tgctcgtgtt gtctatctga
    12721 tttatttttt gatgatgtat ccttcttatt atctatctgt ccattacttg gcagaaaaaa
    12781 aggtatgttg gaggaaaggg aaaatcaaac ggatgtgttt catcattacg ttgtttcagc
    12841 tgtttgtcat tatttttgca gttgattggt atttattagt gggaaagatt ggagcttggt
    12901 atctgattcc ttttctattg tgtctgttgc tttttgtatc ccatagtttt tttagtacgc
    12961 taattgatat ctgcaaagaa gataaagcag atagtcttgt ttatttgatc aaaaggaata
    13021 tctggctgtt gtcaaaaaac gtcaaggggt ttctgtggta tttatgcctt ttgtatggac
    13081 tgttctggtg tatacagcct gattttttac tcactgcatt actgtatcca gggctgccat
    13141 ttgtcacagt attttgctta aaagaaaagg agaatgactt taaatgaaga aaaaatatgt
    13201 acaagtcgga cttggtggga gagcgcgttt tttttatgag gcaattgcct ctacgtatca
    13261 tcaaacttgt gaactagtcg gtttttgtga tgtatctcaa acaaggatgg attatgcaaa
    13321 tcggatactt gtgaacaagt atggacagaa aaaagtaaag acatgtatgc catcacaatt
    13381 tgagcaacta ctggatgacg tacggccggc tgaagtcatt attacgtcag tagatcgaac
    13441 gcatcatgat tatatcattc gtgcaatgaa aaaaggatat gatgttattt gcgaaaaacc
    13501 tctgactatc gatgaggaaa aagctcaaga aataataacg gtccaaaaac agacgggaag
    13561 aagcctgcga gtcactttta attatcgtta tgcaccgtat cacacgaaga taaaggaact
    13621 gatagaaagt gatgtgattg gtgatgtgtt ttctatccac ttcgaatggc tgttaaatac
    13681 gcaacacggg gcagattatt tcagaagatg gcatcgaaat aagcacaata gcggcgggct
    13741 tcttgttcat aaatctaccc atcattttga tctagtgaat ttttggttga acagccgtcc
    13801 ccaaaaggta tttgcattcg gtggattaaa cttttatggg atgcataatg cggagaaacg
    13861 cggagtagaa atattttaca gtcgatgcta tggaagcgaa ctagctaaag ctgatccatt
    13921 tgctatagac ctgtcagcca atgaggaatt gcgagaaatg tatttagaag cagaaaaaga
    13981 cagtggatat attcgtgacc gcagtgtatt ttccgatgat atctctattg aagatacact
    14041 aggcgtaacg gtcaaatatg aaactggtgc gattttgacg tattcgctaa acgcgtatat
    14101 gccgtgggaa ggattcaatg tttctttcaa tggcagcaaa ggacggatcg aaatgaaagt
    14161 gatcgaaaag tcttacatca atgcaggtgg agataaaaag gatgaaggag cagccgttct
    14221 tcagtccatc catgtctatc cgatgttcgg tgagccatat gaggtaatcg tcgaacaaga
    14281 agcgggcgga catggcggag gagatccgcg tttattagat gatctatttg gtgaagaaaa
    14341 agaagaagat ccgtggaatc gggcagcaac tcacgtagat ggtattttgt cgattttgac
    14401 aggaatcgca gcaaatcatt ctattgcttc aggaaaagca gtagtgattg acgagcttgt
    14461 cagcttaaaa gaataagaat agatttacgg aaattacttc ccgtagttta taaaatcttg
    14521 ccttattgcc gataggctgt cttttgaaca ctatttattc ggaaaaaggg gctatgacat
    14581 gctttttgtc atgccttctt tttatgtggg ctgctttttt agataggaga aaataaaaat
    14641 aactatttaa acccgaacaa aaaggaataa tgcattaaaa atcattagat tttacaaaaa
    14701 aataagtaaa ccacgttgtc agtttcagaa agtagtgtta gactgttttc aatatttgaa
    14761 acaacatttt ttattcaaat atggcaaact gccttcacgt gcaaaacagt tgcaagaaaa
    14821 tgaatttaca ctattattag ttttacatac ctacttatat atcgtcataa tatggatgac
    14881 agtttctagc cagtaccgta aatgctggac tataagtaaa agattggcta tttcaccctt
    14941 cttttcttat gataaagtgg agggtgtttt tctttaacag aaacatagta ggtttttgca
    15001 caaaagcggt tatttcggta ttaataaatg ataaaggagc aaataaagtg aaagaattag
    15061 ttgaacgtat tcaaaaagac ggacgtatat taggcgaggg cgtattaaaa gtcgacagct
    15121 tcgtcactca ccaagtagac cctgtattga tggaacaaat gggaaaacgg tttgcagaag
    15181 tatttaaaga acaaggaatc acgaaagttg tgaccattga agcttctggg atcgcaccag
    15241 ctttatttgc tgcaagagaa ttagatgttc caatgatctt cgctagaaaa gcaaaaagtt
    15301 tgacgatgga tgaagaatta ttgacagctt ctgtttattc ttttacgaaa caagtgacaa
    15361 gtacgatttc tatttctcgt aaatttttat cttcagaaga caaagtattg attatcgacg
    15421 atttcttggc aaacggacaa gcggcaaaag gattgatcga attatgccag caagctggag
    15481 cgcaagtaga aggaatcggg atcgtcatcg aaaagtcttt ccaagatgga cgtcagttac
    15541 ttgaagaaat gggattgaga gtcgtttctc ttgcgcgtat tgcttccttg acgaatggac
    15601 aagttgagtt tcttgaggag gacgcataat cgtggaaaaa gaaacacaaa atggaaaagc
    15661 tgcggtttta gggctgcagc atctgctagc gatgtatgca ggagcagtag cggtgcctct
    15721 tttgatcggt acaggactag gctttaatca agaacagatg acttatttga tttcaattga
    15781 tatctttatg tgtggattag cgacattatt gcaattgaca gttaaccgat ttttcggaat
    15841 tggcttgcct gtcgttttag gatgtgcgat ccaagcagta gctccactga tcttgatcgg
    15901 aaccaatgaa ggagtaggag ctatctacgg atctattatc gcttctggga tctttgttgt
    15961 gttagtttct ggtgtctttt ctaaaattaa aaaactattc cctcctatag taacaggaac
    16021 ggttatcacc gttattggtt tgactttgat tcctgtagca atcgagaaaa tgggtggagg
    16081 taatgcaaca gcagctgatt ttggtgacaa aacaaatctg ctacttgcat ttgtgacgat
    16141 tctcctaatt gtaggcgtgc aaatgctggc aaaagggttc attcgttcaa tcgcggtatt
    16201 gattggttta gtcggaggaa gcatcttagc tgctttcatg ggaatgattg atctgagcgc
    16261 gattggagat gcgccgttgt tccatgttcc acaacccttt tattttggta aaccaacttt
    16321 cgacgtatgg tcgattttac taatgatcat catttccatg gtcagcatgg tagaatcaac
    16381 aggtgtatat tttgcacttg gtgacattac tgggaaaaat gttggagaag aagaattgaa
    16441 aaagggatat cgtgcagaag gacttgccgt tatcttaggc ggtattttca atacattccc
    16501 ttatactggc ttttctcaaa atgtcggatt ggttcaactt tcgggaatca aaacaagaaa
    16561 acccatctat ttctctgcat tcttcttgat tgttttagga ttattcccta aaatcggtgc
    16621 gttagcacaa atcatccctg agccggtact tggtggagga atgttagtca tgttcgggat
    16681 ggtggcagtc caaggaatcc gtatgctgtt ggaagttgat ttcagcaatg ataaaaatct
    16741 attgattgtt gctgtttctg ttggatttgg gctgggtttc aatgttacgc cgacattatt
    16801 cagtcaaatg ccgcaaacat tgcaaatgtt cactggaaat ggcatcgtaa tgagtagcat
    16861 tacggctatc atattgaatt tgatctttaa tggattgaaa aaagatgaag aaatattgga
    16921 agaagccttc aagaagtaat agagcaaaag aaaggttgtg aaatcagcgt tttgacctga
    16981 ttgttagaga aacaaacgga aaaatagttc ctcgtatttt gccgtttgtt tgagctaata
    17041 ccgagggtca gctgattgaa caccgtttat ccataaaaag gaatgtgacc taacttttgt
    17101 cacattcctt tttttcatct ttaataaaca cgaacaataa aatgtttttc tttgttaaca
    17161 ttcgaaattt ctttgacatc aaaaaaagtg attgttaaaa tatgaatgta gaaaaactgg
    17221 gaggaaaaac aatgacagca aaaatctctg tgattatggg gagtacttca gattgggaaa
    17281 caatgaaaga agcatgtcaa atattggatg aattcggtgt gtcttatgaa aaaaaggtcg
    17341 tttctgctca tcgaacccca gatttgatgt ttgaatatgc acaaaacgct cgaaaaaaag
    17401 gaatcaaaat cattattgca ggagcagggg gagccgcgca tctgccgggt atggtcgcat
    17461 caaaaacaac tttgccggtt atcggtgtac cggtccaatc acgaacatta aatggtttgg
    17521 attcgcttct ttcgattgtc caaatgcctg gaggagtacc tgtagctact acagcaatcg
    17581 gaaaagcggg agcaattaat gctggactac ttgcgataca gatgctttcc atgtatgatt
    17641 tagaattgga ggctaaattg gctgaaagac gttcaatgtt ggctaaaact gttatagaaa
    17701 gtagtgatca acttggttaa acctttgttg cctggttcaa cgatcgggat cgtcggtggc
    17761 ggtcaattag gtcgtatgat ggcgattagt gcaaaagaaa tgggctttaa agtaggtgtg
    17821 ctggatcctg tgagcgattg tccagccgca caagttgcag attggcacat tgaaagtgtc
    17881 tatgatgaca cctttgcgct gaaagagctt gccagaagaa cagatgtcat tacctatgaa
    17941 ttcgaaaacg tcagtgtaga ggcgttaaat gcgattttac ccatgtcatt tattcctcaa
    18001 gggacagact tgcttgcgat tacgcaagac cgcctgttag aaaaatcttt tttggaaacg
    18061 aataacatcg tgattgctcc ttatgcaacg atcgtcagtc cgacggatat ccaagatgcg
    18121 attgacggga tcggctatcc ttgtgttttg aaaaccactc gaggcggcta tgatggcaag
    18181 gggcagtatg ttttgaaaag tcgggcggat ctagctcctg ccatggacct tttaagagaa
    18241 ggaacctgtg ttttagaagc atggatcccg tttgaaaaag agatttcgat catggtggca
    18301 ggaaatggtc aaggagattt cacgactttt ccggtcgtgg aaaacatcca ccacaacaat
    18361 attcttcatg aaacgattgc tccagccgcg attgatcagg atgtgatcga ggaggctgag
    18421 cgtattgccc gtgtgattgc agaagcagtg agcctttcag gtgttttagg ggtagaaatg
    18481 tttttaacaa aaacaggcgg gctatatgtg aatgaattag ctcctcgtcc tcacaattct
    18541 ggacattatt ctattgaagc ttgttctatg agtcagttcg atgcccatat tcgaggtatt
    18601 tgtggttggc cattaggaga aaaagtacgt ttgataagca atgcagtaat ggtcaatgtt
    18661 ttgggagaac aactgctgga aagctatcgc ttgattgaga ataagccaga ctggcaattc
    18721 catttctatg gaaaaaaaca agcaaaaaaa ggtcgcaaga tgggacatat cactatcttg
    18781 acagaagaca tccagcaaac gctggaagaa atcaaacaaa cgaatatttg ggattagagg
    18841 agaaaaaaca tgttagaacg ttatacacgc cctgaaatgg ggaatatctg gacggaagag
    18901 aacaaatacc gtgcgtggct cgaagtcgag attttagcag atgaagcttg ggcagaactt
    18961 ggtgaaatcc cgcaagaaga tgtgaaaaaa attcgtgagc atgcatcatt tgatatcgat
    19021 cggatcttgg aaatcgaaaa attaacacgc catgatgtcg ttgcgtttac acgagcggtt
    19081 tctgaaacat tgggagaaga aagaaaatgg gtccattatg gtctaacaag tacggatgtc
    19141 gtagatactg cttatggcta tctcatcaag caagcaaatg atattttacg ggaagacttg
    19201 aaacgtttta cggaaatcat tggcgaaaaa gcaaaagaac acaaatatac agtgatgatg
    19261 ggtcggacac acggtgtcca tgctgaacca actacttttg gattgaaact tgccacatgg
    19321 tattcagaaa tgaaacggaa catcgaacga tttgaacatg cggcaaaagg agtagaagca
    19381 ggaaaaatct caggagcagt gggaacgttc gcaaatatcc cgccatttat tgaagagtat
    19441 gtatgtaaac aattggggat ccgtccacaa gaaatctcta ctcaagttct ccctcgggat
    19501 ctacatgctg aatatttctc agcgatggcg cttattgcta caagtattga acgttttgca
    19561 acagagatac gcggattgca aaaatccgaa acacgtgaag tagaagaatt ttttgcgaaa
    19621 ggtcaaaaag gttcttctgc tatgccgcac aaaaggaatc cgatcggttc agaaaatatg
    19681 gctggtctag ccagagtggt gcgcggacac atggtcactg cctatgaaaa tgtcacatta
    19741 tggcatgaaa gagatatttc tcattcttca gcagaacgaa tcatcttgcc tgataccacg
    19801 attttattga attacatgtt aaatcgtttt ggcaatatcg ttaaaaatct aacggtcttt
    19861 cctgaaaaca tgaagcggaa tatgtctgca acatttggtt tgatttatag ccagcgcgtt
    19921 atgctgaaat tgatcgataa gggaatgaca agagaagaag cttatgatct tgttcagcca
    19981 aaaactgcgt acgcttggga caaccaagtg cagttccgcc cgctgttaga agaagatcca
    20041 accatcacat cggtattatc taaagaagaa atagacgatg cctttgatta tcatcatcat
    20101 ttgaaaaacg ttgacgtgat attcaaacga gtaggtttgg aataaagaag agtgagttgc
    20161 aaaattcaat taaaatagaa tagcaaaaat aaccaaaaaa gaagcagatc cgttagcggt
    20221 tctgcttctt ttagtttatt atattttctt atcaataata ggaacctgat tttaaaagcg
    20281 gctacctctt taccgtgcct ctttacatac aaaaaataga ggaattaagc gtaaatggct
    20341 tggctattat aactaaaatg ctggttatgg agatgttgca gcaagttttc gagaaaagcg
    20401 tattccatct cttgtccatt gaataagatc tggatacgat aatgattttt gaaacgcttc
    20461 tgttcatgaa tctgactgat tgaaagttcg cctttgtgtt ctgtctgata aagaaaagtc
    20521 cttttacggc attcagcaca gcgcttggtt gctcggcgga tttttcggaa tgtataaaaa
    20581 ctagtatctt tattgcataa ataaacgtct ttgatcgaca gcaaatcatt atatagacag
    20641 gaacgttctt cagctgttaa ttctgataaa aaaagctgac cttgttttat tgcatcttcc
    20701 atcggcaagt gcctcctcga acccattatc caaattttta gttaattcta atataccatc
    20761 attaagtata aatgtaaacg cttgttgatg gtataagatt ttcgtttctg ataaaacacg
    20821 aacattaaaa gaatctaatg ccttaaatgt acatattttt gttgatttat aaaaagatga
    20881 ttgctaaaat ttaaagtgaa aaaacgaaca aaaaacgatg tatttgcttt taatgtaaat
    20941 aaaaaggaga gacgtacatg gagaagaaag aacttttgta tgaaggaaaa gccaaaaaaa
    21001 tatatcttac ttctgatgag aacttgattt ggatagaata tttagatcaa gcaactgcgt
    21061 tgaatggcgc gaggaaagac cagataagtg gaaaaggaga attgaataat caaatcacaa
    21121 gtgcaatttt ccgttatctg actcaaagag gaataaaaaa tcattttatt gaacaattgt
    21181 ctaaaaccga acaattagca aaaaaagtac aaattatccc tctggaagtg gttatccgaa
    21241 atattgcagc aggaagtttc tcaaaacgtc taggtgttcc cgaaggtaaa gaattcatca
    21301 aaccaatcat tgaattctac tataaggatg accagttgga tgatccattt atcaatgaag
    21361 agcatatcta tttcttacaa cttgcaaaac cggaagaaat cagtgagctg aaagcccaag
    21421 cgttggctat taatgaaatt ttgaccgaat tatttgctaa ggtcggtttg gttttagtag
    21481 atttcaagct agaatttggc cggtcgaatg gacaaatttt gcttgcggat gaagtaacac
    21541 cggatacttg ccgattatgg gatgaacgta cgaatgaaca tatggacaaa gacgtttacc
    21601 gcagagatct aggtgatttg atcccagtct atcaagaagt atataaccgt ctatcggaac
    21661 taactgaaga aaaggagcga gtatgatgta tttcgtaaaa gtatatgtaa cctataaaga
    21721 ttcagtgtta gatcctcaag gggaggcagt aaaaaaagca gtccatcgta tgggatatga
    21781 aacaatcgaa gatatccgaa taggaaaata ctttgagata caacttgctg atgcagaaga
    21841 accaatcgat acagtgattg aaacgatttg tgacaagctg cttgcaaatg taaatatgga
    21901 aacataccga tatgaaatca aaaaaatgga ggaagtataa catgaaattt gcggtcatcg
    21961 tttttccagg ttcaaattgt gacgcagata tgttatgggc agttcgcgag atcatgggcg
    22021 ctgatgccga atttgtacgg catgatgagt cctctttaga aggatttgat ggggttttac
    22081 tgccgggagg attctcctat ggtgactatt tgagatgcgg tgcgattgct cggttttcta
    22141 caatcatgga agaagtgatt cgttttgctg aagaagggaa accagtattc ggtacatgca
    22201 atggattcca gatattgaca gaagcaggac ttttgccagg tgtactgaga cgaaatgagt
    22261 ctttgcattt tatttgcaaa actgtaccat tgaaagtcaa ccccaagacg gcctttactt
    22321 cagagtatga agccgacgaa ataatctatc ttccagttgc tcatggggaa ggaaattatt
    22381 tctgtgacga agaaacactt caagaactaa aaaaaaataa tcagattgcc tttacttata
    22441 atgacaacat caacggcagc gtggaaaaca ttgcagggat tacgaacaaa gcagggaatg
    22501 tgttaggaat gatgcctcat ccagaacgtg cagtagaaga cttgctagga tcagaagatg
    22561 ggaaacggtt ctttgcttcc attttaaaaa acttcggaaa ggtgaccgta tagatgaaaa
    22621 cattgaaaga accgacagct gaagagatca agaaaaaaag aatctatgcc gaatggggat
    22681 tgactgatga agaatatcga atgatcgaag aagatatctt aggaagaatg cctaattata
    22741 cggaaactgg actgttttct gttatgtgga gcgaacattg ttcctataaa aattctaaac
    22801 cggttctacg caaatttcct actagtggac ctcatgtgtt gcaaggccct ggagaaggcg
    22861 caggcatcgt cgacatcggt gatggactag cagttgtctt caaagcagaa agtcacaatc
    22921 atccttcagc cgtggagcct tatgaaggag ctgcgacagg agttggcgga atcatcagag
    22981 atattttcag catgggggct cgtccaattg cgttgctaga ttcattgagg ttcggcgaat
    23041 tagataatcc aagaacgaaa tatttactag aagaagtagt tgcaggtatc ggcggatacg
    23101 gcaattgtat cggcataccg actgtaggcg gcgaaattgc atttgatcct tgttatgaag
    23161 gaaacccatt agtcaatgca atgtgcgtag gtttgattga acacaaacat atccagaaag
    23221 ggcaagccgc tggcgtcggt aattcgatca tgtatgtagg tgcaaaaaca ggtcgcgacg
    23281 gtatccacgg agcaactttt gcctcagaag aatttaatca agaagaagaa cagcaacgtt
    23341 ctgctgtaca agtaggcgat ccttttatgg aaaagctgct gcttgaagcg tgtttggaat
    23401 taatcttgca tcattcggat tctctaatag gtatacagga tatgggagca gcaggacttg
    23461 tttcatctag tgcggaaatg gcttcaaaag ctggctcagg acttatattg acattagatg
    23521 aagtcccaca aagagaaact gggatgacac cttatgaaat gatgttatcg gaatcacaag
    23581 aacggatgtt gatttgtgtt aaatcaggag aggaagaaaa aatccaagaa ctatttcaga
    23641 agtatgatct agatgcagtc actatcggta aagtaacaga cgacgggcaa tatcggctat
    23701 atcatcaagg agaagaagta gcaaatctgc cagtcgatgc attagctgaa gatgcaccag
    23761 tttatcataa agaaatgaaa gaaccagctc gaatcgtaga atttcagcag ttagctcctt
    23821 atcagccagt tatcgaagaa cctggacaag tattgcttga tctgctgcag caaccgacga
    23881 ttgcttcaaa acgttcaatc tacgaaactt atgattcaca agtacaaacc aatacagttg
    23941 tccgtccagg aagcgatgct gctgtgatga gggttagagg gacgaataaa gcgttagcta
    24001 tgaccacaga ctgcaacgcg cgctatctgt atttggatcc aaaaatcgga ggacaaatag
    24061 cagtggcaga agctgctaga aatattattg ctagcggtgg taagcccttg gcgatcaccg
    24121 actgcctgaa ctatggttct cctgataaac cagaagtttt ttgggaatta tcgacttcag
    24181 ctgacggaat tgctgctgcg tgtgaaacgt taggaactcc tgttatttca ggaaatgtct
    24241 ctttatataa tgaaacggat ggtcaagcaa tctatccaac accaatgatt ggtatggtcg
    24301 gattgattga ggatcataaa catatcacta ctcaggaatt caaaaaatca ggggatctaa
    24361 tctatatatt aggtaagacg tttgcagatt ttgatggcag tgaattacaa aagatgcagc
    24421 ttggaagaat cgaaggtgtg atccgaaact ttgatctatc cattgaaaag agaaaccaag
    24481 agttagtatt gacagccatc caaaacggat tgattgaaag tgctcatgac tgttcagaag
    24541 gtggcttggc aattgcactg gcagaatcag ctttcaaaca tcagctaggg atatctgtac
    24601 agtttgaatt gtcctctgct caattatttg cagaaactca atcaagattt gtgttgacag
    24661 tcgcaccgga aaataagaca cgattcgaag aaatgatggg agatgctgct gttttagcag
    24721 gtaaggtgac ggatgaagcg ataatcgaaa tttcagcaac tgacggtcag atcaaaatag
    24781 aaactgcagt agcaagaaag tgctgggagg atgcaatcgt atgtcttatg aagtgaagag
    24841 tttaaatgaa gaatgcggga tcttcggtgt ttggggtcat ccagacgccg ctagtgtggc
    24901 atacttcggg ctccatagtc ttcagcatcg aggacaagaa ggtgcaggta tcgtgtccaa
    24961 tgatcgagga aagcttaagg ggcatcgaga tttggggctg ctttctgagg tattcaaaga
    25021 tcaaagaaaa ttgcagcagt taacaggaga agctgcaatc ggacatgtac gttatgcaac
    25081 agcaggcaat ggaagtgtgg acaatattca gccttttcta ttcaaatttc atgatcagtc
    25141 gatcggctta gcgcataatg gtaacttgat caatgctcgt tcccttagaa aagagttaga
    25201 ggaaaatggg gcgatcttcc attctaactc cgatacggag atattgatgc atttgatccg
    25261 acgaagtgaa gaaacagatt ttttaaataa attaaaaagt gctttgaatg aagtcaaagg
    25321 tggctttgcc tatttattaa tgacagaaac ggcaatgatt gccgctttgg acccaaatgg
    25381 ttttcgtcca ttagcaatcg gacaaatgaa aaacggagca tacgtgattg cctcggaaac
    25441 ttgtgcatta gaagttattg gggcacgttt tataagagat gtccagcctg gtgagatagt
    25501 gatcatcaat gacgaaggaa tccagattga ttgctttacg aaggacacgc agctttctat
    25561 ttgttcgatg gaatatatct attttgccag accagattca aacatcgcag ggatcaatgt
    25621 gcatactgca cggaaaaata tgggacggaa tttagcgaaa gaatcgccag taaaagcaga
    25681 tatggtcgtt ggcgtaccta attcttcttt gtcagccgca agcggttatg cggaagctag
    25741 tggaatccct tatgaaatcg gattagtaaa aaatcaatac attgcccgaa catttatcca
    25801 gccaacgcct gagctaagag agcaaggtgt tcgaatgaaa ttatctgctg tacgaggagt
    25861 agtggaaggt aaaaaggtga ttatggtcga tgattcaatt gttcggggta cgacaagcag
    25921 acggatcgtc catcttttga aagaagcagg ggcaaaagaa gtccatgtga ggattgcttc
    25981 tcctcctttg aaatatccat gtttttacgg gattgatatt caaacgcgta aagaactcat
    26041 tgcggcaaat cacacgattg atgaaatcaa agaatgtatc ggtgcagatt ctttaggttt
    26101 tttaagtgaa gagggtctga tcgaatcgat cggtctggaa aaagatgctc cgtattcagg
    26161 attgtgtatg gcttatttca acggagatta tccgacgccg ttatatgact atgaagaaaa
    26221 atatcaggaa tcactaaaag aaaaagtatc gttcttttaa aagcagacca agaagggagt
    26281 atatgaattc aatggcaaat gcatacgcaa aagctggcgt agatgtcgaa gctggatatg
    26341 aagtagtaga acggatcaag aagcatgtca aaaaaacgga acgtttaggt atgatgggcg
    26401 ctataggagg attcggcggt tgttttgatt tatcggtctt tcaattaaaa gagccagtcc
    26461 ttgtctcggg gaccgatggc gtggggacga aattagcagt ggcaatcgaa gcagacaaac
    26521 acgatacaat cgggatcgac tgcgtagcga tgtgtgtcaa tgatatctta gcacaaggcg
    26581 cagagccgtt atatttctta gactacttag caacggggaa aaatgatccg ttacgtttgg
    26641 aaaatgtagt aaaaggtgta gcagatggat gtgttcaagg aaatatggcg ctgattggcg
    26701 gagaaacagc tgaaatgcct ggtatgtact caggtaatga ttatgatcta gccggatttg
    26761 ctgtagggat agctgaaaaa tctcaattat tgacaggaga aacgatcaac ccaggagatg
    26821 tgttgcttgg aatagcatct agcgggattc attcaaatgg gtactctctt gttcgcaaaa
    26881 ttttctttga gcagcagcag ttttctttat catcaaaact gccagaattg aaaaaaacac
    26941 ttggcgaaga attgcttgag ccaacgaaaa tctatgtttc ttccttgctt ccgctgatca
    27001 aagaaaagaa aataaaagga gctgcacata tcactggtgg agggtttgta gaaaatatcc
    27061 cgcgaatgtt accagaatct atggctgcac aaatcaaact tggctcatgg ccgattcttc
    27121 caatattcgg ctgtttacaa aaatatggtg aaattcctga gatggagatg tatgaaatct
    27181 tcaatatggg catcggtatg gtcctggcag tcgaagaatc gaaagtaaaa gaaatagtac
    27241 acgaaattga gcaagctggc gaaaaagttt atgaaatcgg gaaagtgatc gccaaagaaa
    27301 aacatcgtgt ggtgtttata ggagaagaac gatgagaatc gctgtttttg cttctggaaa
    27361 cggaagcaac tttcaagcgc tagccgatta tctatcgaaa aaaggattgg aatcttcgat
    27421 tgactggttg ttttgtgatc agcctgaagc atatgttttg aaacgtgcaa ctgcgttgag
    27481 tgtaccggca gattgttttt cacctaaaga atttgattca aaaaaagagt atgaagaagc
    27541 aattttgcat aaattgaaag aaaaaaagat cgatttgatc gtattagctg gatatatgcg
    27601 aatcattgga ccggtattgc tgaaaaatta tgataaaaga atcatcaata tccatccgtc
    27661 tcttttacct gccttccctg gtttgcatgg catacgtgat gcctttgaag ctggagtaaa
    27721 agaaacgggt gtcacaattc attatatcga ccaaggtgta gatactggac caatcatccg
    27781 gcaggaaaaa gtgagaattg aacaggagga cacgttcgat tctttagaag aaaaaatcca
    27841 tagagtagag catcgaatct atccagaagt gattagtgaa attattgaaa atggaggcta
    27901 tctgaaatga caagagcatt gatcagtgta tcagataaaa ctgggattgt agcattcgcc
    27961 caaggtttga ttgaacaagg agtagaaatc atttcaacag gaggaacaaa gcatgcgttg
    28021 gataaagctg ggatcaaaac gctttcgatc gaagaagtga cagggtttcc tgaaatgatg
    28081 gatggccgtg tcaaaacact tcatcctaaa atccatggtg gtcttttagg aagacgcgat
    28141 ctccctagcc atatggaggc aatggaaacg gaaaatatcc aaccaatcga ctttgtttgt
    28201 gtcaatttat atcctttcaa agaaacgatt atgaaaccag atgtcgaaat agcagaagct
    28261 attgaaaaca ttgatatcgg tggtcctagt atgctccgta gtgcagcaaa aaaccatgaa
    28321 tttgtcacct ctgttgttga tccatctgac tatgaagaag tactttcaga attacgtcag
    28381 tatgggcata cttcactttc tttcagacaa aaactagcgg caaaagtctt tcgtcatact
    28441 gcggcatatg atgcactgat cgctcaatac ttaacggatt gggtggaaga aaaagaaccg
    28501 gaaaaattga cacttacgta tgaacgaaaa caaacgcttc gttacggtga aaatagccat
    28561 caagccgctg ccttttatca gtccgctctt ctggaaaaat attcgatcgc agcagcaaaa
    28621 caactccatg ggaaagaact ttcttataat aatatccgag atgcagatgc tgcgctacgg
    28681 atcatgaagg aatatgacga gccaacggta gtcgccttga aacacatgaa tccttgtggt
    28741 attgggacag catctacgat tttagaagcg tgggaagcag cgtatgaagc agatcctgtt
    28801 tcaatctttg gtgggatcat cgttttgaat cgagaagtag atctgccaac agcagaacag
    28861 atgcataagc tgtttctaga aatcatcatt gccccttctt atacacaaga agctttggaa
    28921 ttactgcaaa aaaagaaaaa catccgatta ctgacagtag actttgcgca ggaagaaaat
    28981 caaaatcgag aagaaacggt ctcagtctta ggtggacttc ttgtacaaga tcaagatctg
    29041 gcaatagaaa atgaagaaga gtggaaagtc gtgacaaaac gtaagccgac tccagcagaa
    29101 gcaaaagcat tagcgtttgc ttggagagct gtcaaacatg tgaaatctaa tgcgatcgta
    29161 ctggcaaacg aacgtcagac agtaggaatc ggagccgggc agatgaatcg agtcggttca
    29221 gtaaaaattg cgattgaaca agcgcaagag aaaatggaag gtgcagtttt agctagcgat
    29281 gcttattttc caatggatga ttcggttgaa tatgcagcca aacatggtat aaaagcaatc
    29341 attcagccag gaggaagcat caaagaccaa gattctattg atatggcgaa taaatacgat
    29401 ctagcaatgg tctttactga tgttcggcat tttagacatt agagaggagt cggataaatg
    29461 aacattttgg tgatcggtgc aggtggtcgt gaacatgcaa taagtaaaaa acttttagcc
    29521 agtccgaagg tggatacggt gtattgtgcg ccaggaaatc cggggatgac aaaagatggt
    29581 atcaattgtg tcgctatcag tgagcgagat cattcagcgt taatccattt tgttaagaaa
    29641 gaggctattg attggagtat cgttggtcct gaagttcctt tgttaaatgg gatcgtcgat
    29701 gattttatgg cggaaggatt gaaaatcttc ggtccgacaa aagcagcagc tctgatcgaa
    29761 ggttcgaaaa gttttgctaa acaattaatg gatacttatc aaatcccgac agctaaatcc
    29821 agaagttttg aatgttacga aaaagcagca gcgtatatca aagaaaatgg tgcgccaatc
    29881 gtagtgaaag cggacggctt agcagctggg aaaggtgtag tcgtggctac cacagtgaag
    29941 gaagcgctgg atgcggcaga acaaatgttg aaccagcatc gatttgggga tagtagcaaa
    30001 aaaatcgtcg tagaagagtt tttggcagga gaagaattct cccttttggc gtttgtccgt
    30061 aataccaagg tgtatccgat ggtcatttcg caagatcata agcgggcatt tgataacgat
    30121 aaagggccaa atacaggcgg aatgggtgct tattcaccag taccgcaaat ttcaaaaaag
    30181 atgattgaaa aagctgtgga agaagtttta aaaccagcgg ctttgggaat gaaagaacta
    30241 ggacgctctt tcacgggtgt tttatatgcg ggtttgattg ctacgaagga aggaccaaaa
    30301 gtgatcgaat tcaatgcgcg tttcggtgat cctgaaacgc aagtcgttct ttctcggttg
    30361 aagagtgact ttgcgcaagt aatcgatgat ttattagaga atcgtacagt agagttaaag
    30421 tggcagcaag aaggatacaa tgtaggcgtt gtggttgcag ctgccggtta ccccgaagaa
    30481 tatgagacag gaatggttct accagatttt ctggaagaag agctttctgt ttactatgct
    30541 ggagtttcct caaataacgg caaactagtt ggagcaggag gacgtcttta tttagtagaa
    30601 gcatacggtg agacgatcaa agaagcacag aaaaaaattt acaccgcgtt aatggataaa
    30661 acgacagatg gaacatttta tcgtacagat attggagaaa aagctgccaa tagcgaactg
    30721 atcgtgaagt aaatcagctg cttccattta taaaaaatac aacaaacata atcggaagcg
    30781 ccgattttag tgaagccttt tcataaagag tgtggtacac ttttgttgtg tatataaata
    30841 aggaggagtt cagatgaaaa aaattcaatt aggtacttct gatcagaaag cagctgcagt
    30901 gatcttaggc tgtatgcgta tcaacaatgc ggaagatcca gtaaaagtga ttgagacagc
    30961 ttatgaaaac ggcattgatt tttttgacca tgcagatatt tatggaaacg gtgaatgtga
    31021 agaaatcttt gcagatgctc tagcaaaaac atcaatcaaa cgagaagatc tttttattca
    31081 aacaaaatgt ggaatcgttc caggtgtgat gtttgatttt tctaaaaaac atatcattga
    31141 atctgtagaa aaaagtttga cccgtctgaa aatggattat gtggatgctt tacttcttca
    31201 tcgtccagat acattggtag aaccagagga agtagctgaa gcgtttgatg aattggaacg
    31261 gtcaggaaaa gttcgttatt ttggcgtcag caatcaaaaa ccaatgcaaa tcgaattgct
    31321 gaaaaaatat gttcgtcaac cattagtagc taatcagctg caatttggct tgaaacatac
    31381 tggaatgatc gatcaaggga ttcatgtcaa tatgacagat gaagcaagta tcgatcatga
    31441 cggtagtgta cttgattatt cccgattgca tgatatgacg atccaagcat ggtcaccata
    31501 ccagtatgga ttttttgaag gtgtatttat cgggaacgaa aaattccctg aattaaacca
    31561 aacattagaa gaactagcag ctaaatatca aacgacacca actggcttgg cttctgcttg
    31621 gattttacgc catccagcca atatgcaagt gatcgccggt acgatgaacg tagggcgaat
    31681 caaggaaatc gctcaagctt cagaaattaa tatgtctcgg gaagattggt acgaattgta
    31741 ccgtgctgct ggaaacattt taccttaaag attgtcataa gcaggtcaaa agggctataa
    31801 attaaaaaga cagaataact tgaaagctgt tttcagcttc gagatattct gtcttttttt
    31861 attgcaaatg gaaagagtat cattgtacgg atttcgttta ttagttattt ttgtttttaa
    31921 ggaaaagctg tgtccgatat tcttttggag aaatcccttg ttttttctta aataaataac
    31981 tgaaatagtt agggtcatta taaccaattt caaaagcaat atctgaaata cgatagtctg
    32041 attgctgcaa taattttttt gctaattcga ttctttgttc tgtcagataa tcgatgaatg
    32101 tttggttaaa cgattgagaa aaaatcgtac tgaaatgaga gggacttaat gcaacttgtt
    32161 gagcgaccca agttaaggac atatctggat cggtaaaatt ttctttgata aaatccaatg
    32221 catggttcaa aattggctga tatttgttcc ttgaaggctg tgagggcaga gcagttaaga
    32281 aagaaatgaa catataaact gtcgaacggt aaagcgagtc gtcggcgctg attgctaagc
    32341 gttgactggt ggatgtgcaa tgaaccattt cttgcgggat tttcttttct ttcaggcaga
    32401 catgctggag tagcttagtc aagacaaata aacgatataa tcgtgttcga gttggtgtgc
    32461 cttgtggttt cgtcaagtgt tcgatgtact tagatacttc gtttttcgat aatgaagaca
    32521 aatcaagtga gaggtcaaag taaaagacag aatcaactgg aagtttcgct tcttgaatca
    32581 aatcctcata gctgataatt tgttctttaa gggaactggc ggtttcaagt atcatctgcg
    32641 tgatctcaaa agatgcaggt aattcgctga ttctttctgc gacaggacca atggcgacag
    32701 aaaaattatt tttttgtccc ttttctaatt catggaccaa cgtttgggct acctgataac
    32761 tggtctcgat cacattttct tcaagagaat caactattaa aaatttgatg aatcgagagg
    32821 aaacagcaga gaagatcact tgttgattct ccctaaataa aaacttcaaa taatcactga
    32881 gatgagcgta atcttcaaag tctgtactga cttggtttgc tgctaataat acggtcattt
    32941 ttttccctgc taaagagcga ttgaaagatt cagcaagttt caatgtttca ctgatcgtaa
    33001 gtgagccttt gaacaaaccg ttcaagaaat gatttttttt caattcgaaa acaagatcag
    33061 aagaagtgtg cgtttctttt tgctgcaaat ctaaagcagt tactgctttt tctattgctt
    33121 gtttcaattc gctactttta atcggtttaa gcagataatc atctgcctgt acctggattg
    33181 ctcctttcat ataatcgaaa tcgtcgaatc cactaataaa tatgagacga gtccaaggaa
    33241 gtagtttcct tgcttctttt gaaaaagtca acccgtccat gaaaggcatt tggatatcag
    33301 tcagaatgat gtcgggctgt aagtcgataa ttgaagacag tgccatctca ccatctccag
    33361 cttcgccgac ataagtgatt ggataataag aaccaaggac agttatttgt tcccgcaagt
    33421 tattgcgaat gaggtattcg tcttcgatga taaaaacttt atacatgttt gtttcctcca
    33481 attgttttgg gagatagagg gatcatcagt gtgacagttg ttccttctcg gtatttgctg
    33541 tcaatcgtga taggtttttg atcatttccg taataaagaa ggagacgctt gtttacgttg
    33601 taaagtccgt agccgatact gaaatctgaa tcgatccctt tggctaactc tttacgtatt
    33661 tctgccagtt tttctgcagg gatgccaatc ccgttgtcta taactttgaa ttggatcatc
    33721 tcatcaattt tgcatgcggt aatacgaata aatcctttgt gacggacgta cttcgttcca
    33781 tggtatacag cattttcaat caatggctgc aagatcattt ttaaaataga aacaggaaaa
    33841 agtgtttctg gtatatcgat cttatagtca agcatatggc cgtatcttat ttttaagatg
    33901 actagataat cattgatatg gctaatttct ttttcaagag gaatccagtc ttgtcctttg
    33961 cttaaagacg tacgaaaaaa gccggaaagt gcatgggtca tagtcagagc ttcttcaaag
    34021 cagtcttgtt cgatcaaaga aataatggca tccaaactgt tataaataaa atggggagta
    34081 atctgagctt gcaatacacg aacttcactt tgtgctaaat ggtattgttt caaagcgttc
    34141 tcccgtaaag actggtttag atcttccgcc attttattca tgctagcggt aagtgtttgt
    34201 aattccagtg tttccggcag ggttaaacga aagccaaggt tccctgaaga aagttgttct
    34261 gacatctcga tcagctgatt gatcggttct tggacctttc tctctaaaaa gcgacgattc
    34321 tttttcgcat agaaaaaaat ggtactaaga actccaagtt ccactattgt taatagaatg
    34381 agcgagcgga caagtgtcgt attttttttg cttgctaagt cgatctccac acgaacaaaa
    34441 tcttgtaaga tatctgacag cagttgagtg acagaatcta cctgattgat gatctcttca
    34501 ttggtatcca taaaatcttt tgccaagata ttattgatca gctcgttttg ataatcttcc
    34561 agtgtatcga tcgtacgtag cgatacatct aacgtactaa tttctttaag tgtatttgta
    34621 ttatttttga tctctcgaat atcattccta agttcttcta ggatattttc ctcattgaac
    34681 tcatctacgg ttacttggcc agaaatcaag tcccacatac tttccaaaac tttttgatca
    34741 accttcaaag caattgcatc tgctttttgg atatttgcga tcgtctgttg ataaatcaaa
    34801 agctgttgcg aatataacag tatgctcagt agcaaaggga taacagcaaa tagaagcatg
    34861 gctttgtttg cttgggcaaa aatctgtttt atgcttaaat tttttgtcat gatacttctc
    34921 cagtcaataa tttctggttg ggatgttagc tatattctgc tgggaaaaga cagtctcgga
    34981 aataaaaata tctttaggta atgtttttcc tgtttgaata tttccaaaat aacgagtaac
    35041 ggtattggct acataccagc cgacatcagg tttacactcg actacacaat tgacgattcc
    35101 tgagcgtagc ttctcgatca tatcttcttg tccatcaata gttacaatga tcaaatcttc
    35161 tccaggtttg attgatgttt cttttatcgc agctaaagca ccgtgagtca tttcatcact
    35221 atggctgtat aatacatcga tttcggataa atcatttgtt tctaaatatc gtttcatctg
    35281 gtcttttcct ttcatccgaa taaaatcacc gacaattcgg tgaacaatag tcatcggagg
    35341 tttatggtcg atggttccat ctttttttag ggttggttta cgattgattg attccaaaaa
    35401 tccttcggag cgtagcttgg tcggtgtgga gttctctgaa ccagcaagtt ctaggattct
    35461 gatttcctgt ttctttgaat ttgtgaagtg attgctgaca aataaacccg cccgatttcc
    35521 ttctgctttg aaactggggc caatgtgggt gacgaaaagc tcgggatcat tcgtttgaat
    35581 atgtctgtcg acaagtatga cagggatccc tgctcgcttt gcttctttta atacagagtc
    35641 ccacccggat tcttgcaaag gagtaaaaat gatcatatcc actttatagg caatgaacgt
    35701 ccgtatatct tggatttgcc gttcctgatt catgtagcca tttcggtaga gaacttggta
    35761 atcttctttt tcaagcgcag tgataattga ttcagtatgc tttttccgcc agctactctc
    35821 cgttcctgat tgcgaaaaac caacgactag tgggtcaggt gcttcctttg tattaggtgt
    35881 acaccccatc atttggtaag cgattacaac aagggctaaa aaaaataaaa cacgtttcac
    35941 tttaaatcac tccatctgga tttgttagta ctatcttatc agataaaagg gaaaatcaaa
    36001 gtcaaaagga atcaatcgaa aaaaatccaa gaaacttaaa aaaatccaag acaaaaaaag
    36061 tacacctaaa aaatttaaag taaatcccat aaaaaaatca atgttaacgc tttctttatt
    36121 agctatactc ggtgtatcaa ttgaaatggg ggataaaaaa tgaaaaacaa atttagaaag
    36181 attgttggga ctatcgctat tttgaccgca agcattctat taagtgcgtg tggcaattca
    36241 ggatctgcag atgatagtac ggggtatgta ggaatcgcta tgccgaccaa atcagcagaa
    36301 agatggatcg ctgatgggaa caacatggtc tcggaattag aaaaactggg ttataaaaca
    36361 gatcttcaat atggagaaga taaagtagaa aatcaagtag cacaaataga aaatatgatt
    36421 acaaaagggg tagatacact tgtgattgct tctatcgatg gttcggcttt aactgatgtg
    36481 ttagctaaag caaaagaagc agatatcaag gtgatcgcct atgatcgttt actgatgaat
    36541 tcagaaaatg tcgattatta cgcgactttt gataattttg gagtaggtgt atcgcaagca
    36601 gcttatattg aagagcactt aggattgaaa gaaggaaaag gaccattcac gattgaatta
    36661 tttggtggct cgcctgacga caacaacgca ctaatcaatt acaacggagt gatgtctgtt
    36721 ttacaaccat atatggacaa cggtcagttg gtcgtgccat ctggacagac gagtttcagt
    36781 caaatcgcta cgcttcgctg ggatggttct acggcgcaag caaggatgga taatctgtta
    36841 agcgcaaatt atactgacca aacattggat gctgttttgt cgccatatga tccaataagt
    36901 ttaggaatca tttcgtcatt aaaaggcgtg gggtatggga gtgaaagtaa acctttacct
    36961 gtgattactg gacaagatgc gacagttgct ggcgttaaat cgatcattgc cggggaacaa
    37021 acacaaacga tctttaaaga cacacgtatc ttagcaaaaa atacgattga aatgatcaaa
    37081 gcaatcagtg atggagaaga ggttccggtc aatgatactg aaacttatga taatggggtg
    37141 aaaactgtcc ctacgtattt agcaaataca gtatctgtcg ataaagataa ttatcaagca
    37201 gagctgatcg acaccgatta ttataaagaa tccgatctga aaaattaaga ggaaccaaac
    37261 aaggaactaa agagatctaa gtcagagaga ggataagaat tatggcggat tgtattttaa
    37321 aaatggcaga tatcaccaaa aaattttctg gaatccgagc gttagattgc gtgaatctga
    37381 tgattgaacg tggggaaatc catgcattgt gcggagaaaa cggagcggga aaatcaacat
    37441 tgatgaatgt cttgagcggc ttgtatccat atggctccta tgacggggaa atcttttatg
    37501 atggaaagct gtgtcaattt aaagatttga aagatagtga agaaaaaggc atcgtcatca
    37561 ttcatcaaga attagcacta agtccttatc tttcaataaa agagaatatt tttctcggaa
    37621 atgaacaagc agaaagaggc gtgattgatt gggaactgac agaaaagaaa acatatgaac
    37681 tgctgaataa agtcggtttg tctatcgacc cgaatcttct agtatcccag attggcgtag
    37741 ggcaacagca actagttgaa atagctaaag cattatcaaa atccgtccgt ctgttgattt
    37801 tggatgagcc taccgctgca ttgaatgaag aagaaagcgc gaatttgctg agattgatcc
    37861 gagagttgaa agagcaaggt gtaacctcca tcattatttc acataagctg aatgaaatcg
    37921 tggatatcgc agatcgaatc accattttac gggatggaca gacgattgag acactagaaa
    37981 atgaaatgat cagcgaagag catattattc gcgggatggt gggcagagat ttagttgatc
    38041 gttatcctga aagaaaaccg gatattggag aagtttattt taaagtagaa gactggacgg
    38101 tctatcaccc tcttgatcac gagcgaatca taaacgacca catcaacttg catttgaaaa
    38161 aaggagagat tgtaggaata gctggactga tgggagcggg cagaactgaa ttcgctatga
    38221 gtctatttgg acattcttat ggaagcaaga tatccggacg tatattaaag gaaggggcag
    38281 agatatcagt taagaccgtt ccttcagcca ttgcaaacgg actcgcttat gtctcggagg
    38341 accgaaaatc tttaggactg aatctgctga tggatattcg ggaaaacgtg acgctgagca
    38401 gtttggataa aataagtcaa aaaggagtat tagataaaga aaaagaagta actgaggcag
    38461 aaaatttccg gaaaaagatg cgtataaaaa caaactccat ccatcaaatt gtcagtagtc
    38521 ttagtggtgg gaatcaacaa aaagtcgttt tatccaagtg gctgatgaca caacccgata
    38581 ttcttttttt ggatgagcca acccgtggga tagatgttgg ggcaaaatat gagatctaca
    38641 cgattatcga agaaatggca gcaaatggca aagctgtctg tcttatctct tctgaacttc
    38701 ctgaaatatt agggatgtgt gatcgaatct atacgatgaa cgaaggacgt attacaggag
    38761 aagtctcgcg agaagatgca gatcaggaag tcttgatgaa attaatgacg aaagaagagg
    38821 tgggttaagt tggaacaggc aaaaaatctt acggataaaa aagagaaaaa actaaatttg
    38881 aaaagtagag cagctgatct ttttggtcgc tacagcatgg tgatcatttt agcgggacta
    38941 cttattgcgt ttcaattgat gacggatggt attttctggc gtccattgaa tataactaac
    39001 cttgttctgc aaaatagcca tatattagta ctagctgcag ggatgttatt agttgtgctc
    39061 ttaggtcatg tggatttgtc ggtagggtca gtgatggcgt ttgttggtgc aattgcgggc
    39121 atgatgatgg tcaatcgcca aatgaacccg tggatgacag ttatcgcatg tcttgcagta
    39181 ggcgggctga tcggcgcatg gcaaggtttt tgggtagctt atgcagggat accggcgttt
    39241 atcgtgactt tagcaggatt gctgatgttt cgtggactga cacaagttgt tctaggaggc
    39301 caatctctag caccttttcc agctgttttc caaaaaatgt ctacgggtta tttaccagac
    39361 ttaagcgaag gaagcagcct tcacttcttg acattgattt taggaatgat catcacgatc
    39421 gtgttagttg ttggtcaatg gcgaacaaga aatcgtcgga aaaatcattt atttgaagtg
    39481 gaatctccaa cagctttctt ggtaaaggcg gtatttacag cagtcgttgt actaggaata
    39541 acttatatct ttgcctctta tcaagggtat ccgattattt tgatcattct tggtttgatc
    39601 gttgcagcat atgggttttt gacaaataaa acagtagcag gtcgacagat ttatgcgacg
    39661 ggcggaaatc gaaaagcggc tgaactatca gggatcaaaa caaagcgaat cacgttctgg
    39721 gtattcgtca acatgggagt catggcagct ttagctggat tagttttagc tgctcgattg
    39781 aatgcggcaa cacctcaagc aggtacatcg atggaactgg atgcaatggc ggcagtatat
    39841 tttggtggtg cttcgacttc cggcggcatt ggaacaatca tgggtgcgat tgtcggtggc
    39901 ttggtcatgg gtgtattgaa caatggcatg tcgattttag gcgtcggggt agactggcag
    39961 caggcaatca aaggattgat cttgctttta gccgttgtgc tagacttgta taataagaaa
    40021 aaacgaacaa cgtaggagaa cgccggttat tcagaaaaaa gactgtgaca agacatttgt
    40081 cacagtcttt ttttcgctaa aagcgaacat tcattgtttc gtttgtatag actaaaaata
    40141 atatatgttt ttttaccatt cgtataaatt ctctttttgc tcttgatttt tttgcttaat
    40201 tgagttaaat tacatagggc gctaaaaaaa gccttgatta aattaataat gttcgtgttt
    40261 tgctggatgt gaacatcagg aggataatgt aatgaaagta tttgattacg aagatattca
    40321 attaattcct aataaatgta tcgtaaatag ccgttctgaa tgtgatacaa cggtcacatt
    40381 aggtaaacat acattcaaaa tgccagtagt gcctgccaac atgcagacga ttattgatga
    40441 aacaatcgca gaattcttag cggaaaatgg ttatttctat atcatgcatc ggtttgatga
    40501 agcagctcga attccgttta tcaaaaaaat gaaaaagcgt ggattgatta cctcgatcag
    40561 tgttggcgtc aagaaagagg aatattcctt tatagagaag ttggcagaag aatcgctaaa
    40621 tcctgattat atcactatcg acatcgcgca tggacatgct aactcagtta ttgatatgat
    40681 tcagcatatt aaaaaatatt tgcctgaaac ttttgtgatt gccggtaatg ttggtacacc
    40741 ggaagccgtc cgtgaattgg aaaatgcggg cgcagatgca acaaaagtcg ggattggtcc
    40801 aggaaaagta tgtattacaa aaatcaagac aggctttggg actggaggct ggcaactggc
    40861 agctcttaga tggtgtgcga aagcagctcg caagccgatc atcgctgacg gtgggatcag
    40921 aacacatggc gatattgcaa aatctgtccg ttttggtgcg acaatggtca tgattggttc
    40981 attgtttgcc ggtcacgagg aatctcctgg cgaaacaaaa gtcgaaaatg gaatcgtgta
    41041 taaagaatac tttggttctg cttcagaatt ccaaaaaggc gaaaaacgaa atgttgaagg
    41101 aaagaaaatc tggatccaac acaaaggatc tttgaaagat acactagtgg aaatgcaaca
    41161 agaccttcaa tcatctattt cttatgctgg aggaagagat ctggaagcca ttcgaaaagt
    41221 tgactatgtc atcgtgaaaa attcgatttt taatggagac acaatctaaa aaataaataa
    41281 aatattagga tgttgtgaaa aagttgtcct gcattaagta ataagaacag ccaaacaaaa
    41341 atagttcttt atatgttttg tttggctgga attattatcg cagatacaag cttttgaaca
    41401 tcattattca gaataaggga ctgtgataag atttttgtca cagtccctta tgttttgcaa
    41461 aaaaagtttt tcttttttct cgtaaattcg gtaaaatcaa aagaaaaaat agacaataga
    41521 aaagagaagc ttatgtggga aaaagtagag aaaaaacaag gaccgattta tcgtcaaatt
    41581 atggaacaga ttatgagaga aatcgaaaac ggaaggatca atcctggaag tcaacttgaa
    41641 tcagaacgaa aactagggca actttttggt gtgaatcgtt ccaccgttgt ccatgcattg
    41701 gaagagctta gggagcttgg ggtactaacg agcaaacgtg gcagcggatg gtttgttaat
    41761 cagactgaat gggggaaatt cgctgttcca agaatcgatt ggcggcaaat gatctctcct
    41821 cgttatgagc agacggactg gtatgagcaa aaaataagag aagcaaagca gatccaaaaa
    41881 acatcgtttc ttgatttata cgctagcgag atgccagatg agcttttgcc gaattttgag
    41941 tttccttctt attctcttga agaaatctta gcggaagaaa aagaaatgga tcttttaggc
    42001 tatactccgt tacgtaacaa aatcaaacgg tatttggaaa gtaaatttga attggcattt
    42061 tcccccaacc agcttcttgt gactggtggc ggtcagcaag caatcttttt gattttacaa
    42121 acgattcttt cagctggaga ggggatagct gtagagtcac cttctttttt ttaccgatta
    42181 cctcttttca aagcttcagg aacgcgttta tttgggatac cgatggatga agaagggatc
    42241 gacttagaaa aactgaaaga atcgatccat aaaaataaat tgaaagcagt gcttatcaac
    42301 ccgaattttc aaaaccctac aggaaaagtg atgagtcaaa aacgacgaga aaagctagta
    42361 gaattgtgtc gacagtatcg gcttccaatt attgaagacg atgtatttac ggatctttca
    42421 tttctaaacc aagaagaagt ccttcctata cgaacgattg atccggataa tgtgttatac
    42481 gtcggttcgt tatctagggt tttaggaaaa acaacgaaaa tcggttgggt gataggacct
    42541 gagactttta tctcgcaact agctcaagcg caagaaatga tggagttttc gatgaatatc
    42601 cttacacaaa tcacagtggc taatgtattt aatgaagcaa ttgatttgaa aatgcaagat
    42661 gtaagaaaaa agttgaaaga aaaaagccga ctgataagcg cttggggaga ttcacaatcg
    42721 tttttcaagc ttcattcaat tttaggtggt tactatgcct ggattacttg ggatgggaaa
    42781 aaactagacc ccgaactagc agaaaaagta gtagatatgg gtcttggaat tgcaccaagt
    42841 ctactttttg ggcaagatat aaatggaatc agaatcaatt acagtaggct ggatgaaaac
    42901 caaataccgt tgttttattc aaagatgaaa attctagaaa agtggttaac agaataattt
    42961 cacaaagtaa atagattaaa gaatataaca gcttggattt aaatttcaag ctgtttcttt
    43021 tttttcggaa aaagaatacg atataaagaa actaagaaaa aaccattatt tgtacgtccg
    43081 ttcacgatgt gtaaataaaa taaatgtttg caacaaaact gtataacacc aacgttttta
    43141 aatagaaaca gatggttgta agtttgatta gaaaatagaa aaaatgcaga tgaaaaataa
    43201 aacgctttcc ttattgtgat tttattcact aatgctgtta atatgcggat tggcataatt
    43261 tcctttcatc gtgaaaattt gtgcttttag cttgtatttc tcataaatcc atgataattt
    43321 attaatgaag acggttaaat agtgatacag ttttattcac tgtttcaatc gcagaatgaa
    43381 gagattttct tatagatgag agaggaatgt gtgacaaatg gcaaacaaaa aagcaccgat
    43441 cgacttccaa gcactgctag atgaagtcaa tgcagatttc ccagtatatc aagcacttga
    43501 tcaagatgga aacgtagtta acaaagattt agtacctgat ttatccgatg atgaactcgt
    43561 tgaattaatg actcatatgg tatggtctcg tgtattagac caacgttcaa cagcgctgaa
    43621 ccgccaagga cgcctaggct tttttgcacc aactgctgga caagaggcaa gtcagctggc
    43681 tagtgcttac gcatttgata aagaagatgt tcttcttcca ggttatcggg atgtacctca
    43741 actagtaaaa catggtttac ctttatctca agctttttta tggtctcgag gacatgctgc
    43801 aggtaattct tatccggaag aattgaaagc tctaccacca caaattatca tcggtgcaca
    43861 atatgtacaa gcagccggtg ttgcattagg tttgaagaaa cgaaacaaga aaaatgtcgt
    43921 cttcacttat actggtgatg gcggttcatc acaaggtgat ttttacgaag caatcaattt
    43981 cgctggcgct taccatgcaa atgccgtatt ttatatccaa aacaacgggt atgcgatctc
    44041 tacacctcgt gaaaaacaaa ctgcagcaaa aacacttgca caaaaagctg tcgcagctgg
    44101 gattcctggt attcaagtcg atggaatgga tccgcttgca gtatatactg tatctaaaat
    44161 ggcacgggaa tgggcgatca gtggaaatgg accagtccta atcgaaacac tgacttatcg
    44221 ttatggacca catacactat ctggtgacga tccaacccgc taccgtacaa aagacacaga
    44281 tgacgattgg cagaaaaaag atccattgat ccgcttccgt aaatatttga cggaaaaagg
    44341 actttggtct gaagaaaaag aagaacaagt catcgaagca actaaagaag aaatcaaagc
    44401 agctattgct gaagccgaca aagtaccaaa acaaaaggtt tcagatttct tgaaaaacat
    44461 gttcgaagta tctccacaat caatcaaaga acaaattgca atttatgaag cgaaggagtc
    44521 gaaataatca tggcacaaaa aacaatgatc caagcaatca cagatgcctt agcacttgaa
    44581 ctagaaaatg acgagaatgt cgttgttttt ggtgaagatg ttgggaaaaa cggaggagtt
    44641 ttccgggcaa ctgaaggatt gcaggaaaag ttcggagaag accgagtatt cgatactcct
    44701 ttagctgaat ctggaatcgc aggattgagt ttcggtcttg cgttagaagg atttcgtcca
    44761 gttcctgaaa tccaattctt tggttttatt ttcgaagcaa tggacgaagt agtagctcaa
    44821 atggctcgta caagatatcg aatgagcggc actagaaatc tacccatcac aattcgttcg
    44881 ccatttggtg gcggtgttca tacaccagaa ttgcattcgg ataatttaga aggtttgatc
    44941 gctcagtctc ctggtattcg agtagtgatt ccttccaacc cttatgatgc aaaaggacta
    45001 ttgatttctt ctattagaag taacgatccg gttgttttcc tagaacacat gaaactatat
    45061 cgctcattcc gagaagaagt gccggatgaa gcttacgaag ttcctttgga taaagcagca
    45121 gttactcgtg agggaacaga tgtatctatt atcacatacg gagcaatggt gcgtgaagca
    45181 atcaaagcag cagacaatct tgcaaaagaa aatatatctg ttgaaatcat tgatcttcgt
    45241 acagttgcgc cattagatgt agaaacgatc attcaatccg tggaaaaaac tggtcgcgta
    45301 gttgttgtcc aagaagcaca acgacaagcg ggcgttgccg cgcaagtagt ttctgagatc
    45361 tcagaacgcg ctatcctttc attagaagcc ccaatcggac gagtttctgc acctgataca
    45421 gtcttcccat ttggacaagc agaaaatgtc tggttgccaa atgcaaaaga tatcgaagac
    45481 aaagtaaaag aaatcgcgga attttaagaa aagaagggac gaaacaaagt catggcttat
    45541 caatttaaat tgccggatat cggtgaagga atcgcagaag gtgaaatcgt caaatggttc
    45601 gtcaaaccag gagatacgat caatgaagat gatacattac tagaagttca aaatgacaaa
    45661 tcagtagaag aaatcccgtc acctgttaca ggaacggtta aaaatgtgat tgttccagaa
    45721 ggtaccgtag caaatgttgg ggatgtatta gtagaaatcg atgcaccagg acacgaagac
    45781 aatgaagggg acagtggtgt agccgcagaa tcccaaactc cagctaaacc ggctgctgaa
    45841 cccactgttg atacagaatc agctggatcg tcaagcgaag gagtcttcca atttaagtta
    45901 ccggatatcg gtgaaggaat cgcagaaggt gaaatcgtca aatggttcgt caaaccagga
    45961 gatacgatca atgaagatga tacattacta gaagttcaaa atgacaaatc agtagaagaa
    46021 attccgtcac ctgtcacagg gacagttaaa aacgtgattg ttccagaagg cactgtagca
    46081 aatgttgggg atgtattagt agaaatcgat gctcctggac acaatagtgc accgtcaact
    46141 agtgcgccat cagctgaagc accaaaagaa aaagtggaaa catcaggttc agcaagtgtc
    46201 gttgaagctg ccgatccaaa taaacgtgta ttggctatgc catctgttcg tcaatttgcc
    46261 cgtgaaaaag atgtagatat ctcacaagta actgcaactg gaaaaggtgg cagagtaaca
    46321 aaagaggaca tcgaaaactt cttggcaggt ggtccaagca gtgcaccagc gaaatcagaa
    46381 gcgccagaag cggcagcacc gaaagaagca gctcctgcag cagaaagcaa accagctgca
    46441 ccagcaaaac cattcaagtc caacttagga gacttggaag aacgtgtagc aatgacgcca
    46501 acacgtaaag ctatcgctaa agcaatggtc aacagtaagc atacagctcc acacgtgaca
    46561 ttgcatgatg aagtagaagt gtctaaacta tgggataatc ggaaacgatt taaagaagtt
    46621 gctgctgcaa atggtacgaa acttactttc ttgccatatg tcgtgaaagc tttaacagcg
    46681 actgttaaga aatacccagt attgaatgca tctatcgacg atgcaaacca agaaatcgtt
    46741 tataaacatt actacaatat cggtattgca actgatacgg atcacggact atatgtacca
    46801 aatgtgaaag atgcagaccg caaaggaatg tttgctattg cagacgaaat caatgagaaa
    46861 gcaaaattag ctcatgacgg caaactatct gcagaagata tgcgcaatgg aacaattacg
    46921 atcagcaata tcggatctgt aggtggcggt tggtttacac cagtcatcaa ctatccagaa
    46981 gtagctattt taggtgtagg aacgattgca caacaaccaa tcgtaaatgc tgaaggcgaa
    47041 atcgttgtcg gtcgtgtgat gaaactgtca ttaagtttcg atcaccgtat cgtagatgga
    47101 gcaactgcgc aacaagcaat gaataatatc aaacgtcttt tagctgatcc agagctatta
    47161 atgatggaag gatgataaac aaatggtagt tggagatttt gctgtagaat tagatacagt
    47221 tgtaattggt gcgggacctg gtggctatgt agctgccatc cgcgctgctg aaatgggaca
    47281 aaaagtagca atcattgaaa gagagtatat tggcggtgtt tgtttgaatg tcggctgtat
    47341 tccttcaaaa gcactgatag ctgcaggaca ccattatcaa gagtcactag attctgaaat
    47401 gtttggagta actagtgaaa acgtcaaact agattttgca aaaacacaag aatggaaaga
    47461 aaacaaagtt gtccatacgt tgacttctgg tgttggtttc ctattaaaaa aacataaggt
    47521 tgaaacaatc gaaggagaag cgttcttcgt agatgatcac acactacgcg tcattcatcc
    47581 agattcagct caaacttatt cgttcaataa cgcaatcatc gcaacaggtt cccgtccaat
    47641 cgagattcca ggatttaaat tcggaggacg tgtgttagat tctactggcg gattagcatt
    47701 gaaagaagta cctaagaaat ttgtgatcat cggtggcggt gtgattggtg ccgaattggg
    47761 cggagcgtac gcaaacttag gagcagaagt aacgattctt gaaggctcac cacaaatttt
    47821 accaacttac gaaaaagatc tagtaaaatt agtagaagat gatttcaaga aaaaaggcgt
    47881 aacagtcgta acgaatgcaa tggcgaaaga agcagtcgat aacggtgaca gcgtgacagt
    47941 gaaatatgca gtggacggga aagaagaatc cgttactgct gattatgtga tggtcactgt
    48001 aggtcgtcgt ccgaatacag atgatatggg cttagaacaa gcgggtgtcg aagtaggaga
    48061 acgaggattg atcacggtgg ataaacaagg acgtacgaac gtacctaaca tctatgccat
    48121 cggtgatatc gttccagggg cggcattagc acacaaagcc agttatgaag caaaaatcgc
    48181 tgctgaagca atctctggca aaaaagtagc tgtagattat aaagcaatgc cggcagtagc
    48241 cttcactgat cctgaattgg cttctgtagg tatgacgatc aaagaagcaa aagatgctgg
    48301 tatcgaagca aaagcatata aattcccatt ttcagggaac ggtcgggcat tatcgcttgg
    48361 taaaacagaa ggattcatcc gtctagttac gacgatcgaa gataacgtgc ttattggcgc
    48421 gcaaatcggt ggagtcggcg caagtgatat ggtctctgaa ttagctttag caatcgaatc
    48481 aggaatgaat gctgaagata tcgccttgac gattcatcct catccatctc ttggcgaaat
    48541 cgtcatggat gcttcggaat tagcattagg tttaccaatc catatttgat ttgaataata
    48601 caggcgaaac gcaactgaaa aatcagttgc gttttgtttt ttagaattat caataatatt
    48661 attatgtaaa cttaaaaata tgacaacttc ttggtttatt accacttcct atgctacact
    48721 gaagaggaat atgaaatagg agagttgaag atgcaagaat atcagtatcc acttgattta
    48781 gattggacaa cagacgaaat ggtcattgta atgaagatgt gggaagatct ggaacgtgcg
    48841 aatgaaaaag ggataaacaa tgaagctttt ttgaagactt atcagcagtt taaaactgtt
    48901 gtcaaatcca ttggtgagga aaaacgattg ggacgggaat ttgaaaaagc ttccggttat
    48961 tctttatatc gtacagtgca gcaagcaaaa aaaaatccaa acaaaacttt gagaatgaac
    49021 gggtgagata aatggaacag atgattgatc atatcaagtc ctggcttgaa gaagctggtg
    49081 agaaaatcaa atcttctcaa caaacattaa caatctcgca aaaatcaaat cgaacggatc
    49141 tagtaacaaa tgtcgatcga aacatccaga attttctaat cgaaaaaatc cattcctttg
    49201 atccagaggc gaaaatattg gcagaagaag aagggtgtaa tcaattatcg gatctcgatg
    49261 gaagagtctt tatcatcgat cctattgatg gcaccttgaa ttttgtgatt gaaggggaaa
    49321 atttttgtat tatgctcgct gtttatgaat ccggcgttgg aaagcttggt ttcatttatg
    49381 acgtgatgag aaatgaactt ttttggggtg gaaaaggaat aggcgtgtat aaaaatgatg
    49441 agccattgcc tagaccagca aataaatcct tatcggaagg cttacttggt gtgaatagtt
    49501 atcttttcgg tcataataaa tatggaatcc gagaaattgc ggaaagcagc atggggacac
    49561 gtatgtacgg atgcgctggt cttgaactag tggcaatctt gaaaggaaac cacattgctt
    49621 atatttcaaa ccttagccct tgggattatg cagcgggaaa tgtcttattg gaagagtttg
    49681 gtatggtaac aagcggtctt tttggtactc ctttgacatt tgaaaaaagg gaatactatt
    49741 tagcagggac ttcttgtgca tatgcagaaa tcgcagacct tttgaaaaaa gaaggctgaa
    49801 aaaaaggtag aaatcattca caaaaatgat aaaaacgagc aaaagattaa ttttctcgaa
    49861 aaaatgaaaa cattttacta tatttcatga aaaaagtctg ctataattag cagtcgagta
    49921 aaatttgccc tgtcataaag aaccaataaa aaaggagtta gttacattga attatagaaa
    49981 cgatatccgt aacgtcgcaa ttattgccca cgttgaccac ggaaaaacaa cattagttga
    50041 tgaactatta aaacaatcag atacattaga cgcacatacg caattgcaag aacgtgcgat
    50101 ggactcaaat gcgctagaaa aagaacgtgg tatcacgatt ttagccaaaa acacagctgt
    50161 agactataaa ggtattcgcg taaacatcat ggatactcca ggacacgcgg acttcggtgg
    50221 cgaagtagaa cggatcatga aaatggttga cggcgttgtg ttggtcgtag atgcgtatga
    50281 aggaacaatg cctcaaacac gttttgtatt gaaaaaagca ctggaacaac atatcacacc
    50341 aatcgttgtc gtaaacaaga ttgacaaacc ttctgctcgt cctgagcatg tcgtagatga
    50401 agttcttgaa ctattcatcg aattaggagc agatgatgat cagttagact ttcctgtgat
    50461 ttatgcatct gcactaaatg gtacgtcaag cttgtcagat gatccagcag atcaagaacc
    50521 aaccatggca cctatttttg atacaattat tgaaaaaatc cctgctcctg tcgataacag
    50581 cgacgaacca ttacaattcc aagtttcatt acttgactac aacgattatg taggtcgtat
    50641 cggaatcgga cgtgtattta gaggaacaat caaagtagga gaccaagtag cattaattaa
    50701 attagacgga acagttaaga aattccgtgt aacgaaatta tttggtttct ttggactaaa
    50761 acgtctagag attcaagaag caaaagctgg agacttgatt gctgtctcag gtatggaaga
    50821 tatctttgtc ggtgaaacag ttactcctgt ggatcatcaa gatgcgttac ctatccttca
    50881 cattgacgaa ccaacattgc aaatgacttt cttagttaat aattcaccat ttgccggtcg
    50941 agaaggtaaa ttcgtaaccg ctcgtaagat tgaagaacgt ttgatggcag aacttcaaac
    51001 agatgtatcc ttacgagttg aaccaacaaa ctctccagat gcatggacag tttctggtcg
    51061 tggagaattg catttgtcta tcttgattga aaacatgcgc cgtgagggat acgaattgca
    51121 agtatctcgt ccagaagtta ttgaaaaaga aatcgacggt gtaaaatgcg aaccgtttga
    51181 acgcgtgcaa atcgatacac cagaagaata tatgggtagt gttatcgagt ccttgagctt
    51241 acgtaaaggt gaaatgcaag atatggtcca tacaggaaac ggacaaatcc gtttgacatt
    51301 cttaacacct gcccgcggtt tgattggtta ttcaactgaa ttcctttcaa tgacacgtgg
    51361 ttacggaatc atgaaccata cttttgacca atatctgcca atgcttcctg gtcaaattgg
    51421 cggacgtcac caaggggcgc ttgtttcgat cgatacagga aaagcaacga cttattctat
    51481 catgagtatc gaagaacgcg gtacagtctt cgtagaacca ggtacagaag tatatgaagg
    51541 aatgattatc ggggaaaaca gccgtgataa tgatttgact gtcaacatca cgaaagcaaa
    51601 acaaatgaca aacgtacggt ctgctactaa agaccaaaca tctgtcatca aaaaaccaaa
    51661 acaattgacg ttggaagaat ctttggaatt cttgaatgat gatgaatatt gcgaagtaac
    51721 accagaaagc attcgcttga gaaaacaaat tttagagaaa aatgctcgtg aaaaagcatc
    51781 taaaaagaaa aaataatctg taaaaggatg aagagagaaa ccagcttgtt ataaaaaata
    51841 gctggtttct ttttttgtcc aactcttttc tgtggagaag tcatctttgt caatggacat
    51901 aaattcagaa aatacgccga aagacgttta aaagactttt aaaaaactta tttctagaga
    51961 gtttttgttt cataataaga taatgtttca cgaaaggaga tcaacttgtc cttcgaagaa
    52021 aatcgaagta aaatggcaag agaaaggagc gggaagatga acgacttatt aaaacaatac
    52081 tttggttatg atgagtttag acctggtcaa aaagaaatta tccagaaagt cattgatcag
    52141 gaaaatgtat taggaatcat gcctaccgga agtgggaaat caatctgtta ccagcttcca
    52201 gctttactat tagatgggct tactgtggtt gtgtcccctt taatctcttt gatgaaggat
    52261 caggtggacg cggcgaatca attaggtata cccgccactt ttatcaatag ctcgttagat
    52321 ggttacgaaa cagctagacg cttccaagaa atcgatagac agcagtaccg tttactttat
    52381 atcgctcctg aacgttttat catgccggat tttatccaag caatgaagcg atggaacgtc
    52441 cgtatgatcg ctattgacga ggcacattgt atctctcagt gggggcatga tttccgccca
    52501 agttatttac agatggcgaa ccagctagat caactgccaa atcgtcctgt aattgttgct
    52561 ttgacagcta cagcgactgt gcaagtagct gctgacataa aaagactttt aaaaattcca
    52621 gaaaacaacc atattcagac tggttttgaa cgagaaaatc tacgatttca agtaattaaa
    52681 gatcaaaaaa aagaacaata tctaatcgaa tatttaaaga tcaacaaaaa tcaatctggt
    52741 atcatttatg cagcaacgag aaaagaagta gaccgtctct atcatttgct taaaaaattc
    52801 gatttttccg tgggtagata tcatggtggt ctaaatgaga atgagcgaac agaaatgcaa
    52861 gaagcctttt tatacgatcg cttgcagctg attgtagcga caaatgcatt tggtatgggt
    52921 ataaataaaa gcaatgtccg ttttgttatc cattatcaaa ttcctggctc tctagaagca
    52981 tactatcagg aagctggtcg ggcgggaaga gatggtcttt ccagtgaagc catcctgctt
    53041 ttctctcctc aagatatcca agtccaaaag ttttttatcc agcagtccca aagagaagaa
    53101 gggcaaaagc aaaaagaata tgaaaaatta aaagcgatga cagaatacgt gtatattgag
    53161 tcctgtcttc agcaatacat cctaaactat tttggcgaga caagcagtcc gtgcaaccgt
    53221 tgtggtaact gtttagatga cagagaaata gtagaagtaa cgacagaagc acagatggtt
    53281 ctttcttgct taaaacgtat gggtgagaac tatggtaaac aaatgctgat gaaagtctta
    53341 gcaggatcga aagaacaaaa gctgcaagct ttaggttttg gacatttatc tacctatgga
    53401 ctgatgaaaa accaatcgca aaaagaaacg atgcagttaa ttgaatattt gatatcgaat
    53461 ggctatcttc tgaccatcaa tggtgagtat cctgttttga aagtaacaga aagagggata
    53521 caagtattga aaggacaaga atctgtttat cgaaaggaac ccaagaaagt ccagcagcta
    53581 tcggatgaag aaaccgatac actatttgaa gtgctgcggg aactgcgaac ggatctagca
    53641 tcagaagcag gcgttccccc gtatgtcgta ttttcagatt ccacattgaa agaaatgagc
    53701 cgaatacgtc catcaagcag attggagatg cttcagataa aaggagttgg ccaaagcaag
    53761 cttgataaat atggcgaagc ctttttatct cggataaaaa atgcggactc taacgttctg
    53821 gataagtaga aagaacgaat acgtaaaaat gaataaaaac aactggttaa atctgttttt
    53881 tttgctcctt atgaaaatca aatatgttat aattaaggaa ttagaatggg aggtaagcac
    53941 agatggattc tttgtcgaaa aaacttgcga tccaagtatt aaatgatgaa gcagaacgga
    54001 tcaaacgatt aatccgaaat caaaacaaca gtctctgtat ctcacaatgc aaagccttcg
    54061 aagaagtggt agatacacag atgtacggct tttcccggca agtcggtttt gctgtccgta
    54121 taggagtaat cggaaagaac gagggccagc tgatgttaag tgagctagaa cgagaattga
    54181 atcagttata tagtgaggtt taccaagaaa tttatgataa aaaagaagtt ggcaaggagg
    54241 aatagacttg cctaagaaag tgaaaaaaag gcatctatta gactatagca tcttgattcc
    54301 ctatttaatt ttatgtgtat tgggattgat catggtatat agttctacgt cgtatctatt
    54361 attagaaaac ggacaaaatc cgtccgcatc agtcattaac cagagtattt tttgggtgct
    54421 tagcttgatt gctatcgcac tactttataa aatgaaaaca gatgttttga aaaaccagcg
    54481 tttgatcatg gcagccatcg cagttttgac gattttgctg ctgattgttg tgttttttgg
    54541 taaggagatc aatggggcga aaggttggct gcaaattgca ggattctcga tccaacctgc
    54601 cgaataccta aaaatcattt ctatctggta cctgtctctg actttatcta aaagacaaaa
    54661 cagtgtacaa aaagattttc ttgggactgt gaaacgtcca cttgcaatgg ttatcggact
    54721 tactgcatta gtcgctattc ttcccgattt tggtaatgcg gcagttatct ttttgattat
    54781 tttggttctg cttttagcca gcggagtcaa ttatgtgtat actttgattg taggagtagg
    54841 aggcttttgt ctaagtactt ttacgatctg gctgatcaat atcacaaacg gaaagatctt
    54901 acccggacgt ttacagtata tctataatcg atttgctatt taccaaaatc ctttctcaga
    54961 cgaattaaat aaagggcatc aactagtcaa cggctactat gccatgttca acggaggact
    55021 atttggcagg ggattaggta acagtatcca gaaaaaagga tttttacaag aagcacaaac
    55081 cgattttatt tatgcaatcg tagtggaaga actgggagtg attatgggga ttttgatatt
    55141 agcgttgcta ttctttatga ttgtgcgcat tgtccttgta ggtatccgtt caaaagatcc
    55201 gttcaattcg cttttgtgca ttgggatcgg cgcaatgttt ttgatccaag tatttgtcaa
    55261 tcttggagga attactggcg ttattccttt gacagggatc acgttcccat ttttaagtca
    55321 aggaggctcc agtttgctga tgctttcgat ttgtgtcggc tttgtcctca acatcagtgc
    55381 ggatgaaaaa cgcaagagct taggtttata ataaaaaaag atgtaggagt ggttcgatga
    55441 aaaaagtttt agttgctaac aggggtgaaa tcgctgtacg tgtatttcgt gcttgtacag
    55501 aattaggaat taaaactgtt ggaatctatg cagaagaaga tgaatattcc gtacaccgtt
    55561 tcaaagcgga tgaagcatat ttagtaggac aaggaaaaaa accgattgat gcgtatctag
    55621 atattgaagg gatcatttct atcgcgaaag aatgcggagc tgacgctatt catccgggtt
    55681 acggtctttt gtcggaaaac ttgaattttg cgcaacggtg cgaagaagaa ggcatcattt
    55741 ttgttggacc caaactgcat catttagata tttttggaga taaaatcaaa gcaaaagctg
    55801 cagctatcga agcggggatt gcttcgattc caggcacaga tggaccaatc gcttctatag
    55861 acgatgcctt ggagtttgcg aaacaatacg gttatccgat tatgatcaag gcagctctag
    55921 gcggcggcgg acgcggcatg cgtgtagctc acgatgaaaa gagtgcaaga gagggctacg
    55981 aacgtgcgaa aagcgaagca aaagcagcct ttggtagtga tgaagtgtat gttgaaaaat
    56041 acatcgctaa tcctaagcat atcgaagtac agattctcgg ggatacgcat ggaaacgtca
    56101 tccatctttt tgaaagagat tgttctgttc agcgccgtca ccaaaaagta gtagaagtag
    56161 cgccttgtgt ctctatgaat gaacagcaac gtcaaaaaat ctgccaggca gctgtccaat
    56221 tgatgaaata tgtcgggtat gtcaacgcag gtacagtaga atttcttgtt gaaggagatg
    56281 atttttactt tatcgaagtc aaccctcgcg tccaagtgga acatacgatc acagaaatga
    56341 ttacagatat tgatatcgta acaacgcaac tgttgattgc tcaaggtttg gatctgcata
    56401 aagaaatcgg attgcctcag caagaaggaa ttaaattaaa tggttcagcc attcaatgcc
    56461 gaatcactac agaagacccg ttgaataact ttttaccaga cacagggaaa atcgatacgt
    56521 atcgttcacc tggtggtttt ggcgtacgtc tggatgtcgg aaatgcttac gcgggctatg
    56581 tagtgacacc gtattttgat tcattgctag taaaagtatg tacgcatggt gctacctttg
    56641 aaacagccat tcagaaaatg gaacgttgct tgaaagaatt tagaatccgt ggagtgaaaa
    56701 caaacattcc atttatgctc aatgtcatca cacatcctga attccaatca ggaaatgcaa
    56761 aaacaacctt cattgatagt acagcaacat tatttgaatt tcctcgacta cgagaccgcg
    56821 gaaacaaaac gatgaaatat atcggtgaaa ttactgtgaa tggttttcca ggtatcgaaa
    56881 gtggcgaaaa accattttat gaagaaccac gtatgcctaa agatctaatc acaagaactg
    56941 attacgtaac ggctaagaat gtattagatg cgaagggtgc cgatgctttg gtcgaatgga
    57001 tcaaaggaca ggaaaatttg ttgttgacgg atacgacatt tagagacgcc catcaaagtc
    57061 tgctagcaac tcgtgttcgg acaaaggatt tcaagcagat tgcacgtttg actggagaag
    57121 gtcttcctga actcttttca agtgagatgt gggggggagc aacatttgat gtggcttacc
    57181 gctttttgaa tgaagatcct tggcaacggt tgagaaaaat tcgttcttta atgcctaata
    57241 cgttgctgca aatgcttttc agaggttcaa atgccgttgg gtattctaat tatcctgaca
    57301 atgtattggt ggaatttgtc aaagaagcag cagcgcaagg aatcgatgta tttcgaatct
    57361 ttgatagttt gaactggact ccacaaatgg aaaaaagtat ccaagctgta cgggatactg
    57421 gtaaaatcgc agaggcagct atttgctata ctggcgatat caacgatcca agtcgggcaa
    57481 aatacaatgt tcagtattat aaagacatgg caaaggaact agaacaactg ggtgcgcata
    57541 tcattgcaat caaagatatg gctgggctgc ttaaaccgca ggcagcttat cgtttgatta
    57601 gtgaattgaa agaaacgaca gatctgccaa tccatctgca tactcatgat accagcggga
    57661 atggaatcat cacttattct gctgcttcaa aagcaggagt agacatcgtt gacgtggcaa
    57721 tgagtgcaat gagtgggaat actagtcagc ctagcatgag cagcttgtat tatgcactgg
    57781 tcaatggtcc acggctgcca gaaatcacga ttgaaaatgc acagaaatta aaccattatt
    57841 gggaagatgt gcgcatgtat tacaaaccgt ttgaaaacgg cttgaatgct cctgaaacag
    57901 aagtatatat gcatgaaatg ccaggaggac agtattcgaa tctacagcaa caggcaaaag
    57961 ctgtcggatt aggtcatcgc tgggatgaaa tcaaacaaat gtatcatact gtcaacttga
    58021 tgtttggcga tatcgttaaa gtaactccat catctaaagt agttggagat atggcactat
    58081 ttatggtcca aaatgatctt acagaagaag atatttatga aaaaggcgaa acactaagtt
    58141 tccctgaatc tgttgtgact ttcttccaag gagaactagg gcaacccgtt ggcggatttc
    58201 ctaaaaaatt gcaaaagatt attcttaaag gcagacctgc attgaatgaa cgtccaggtc
    58261 ttttggctga atcagtcgac ttcaacgaag taaaaaaaga attggcagaa aaaattggct
    58321 atgaaccaaa acaagacgaa gtattaagct atttgatgta tccgcaagtc tttttggact
    58381 atcaaaaagc atataatcaa tttggcgatg ttacattact ggacacacca acatttttcc
    58441 aaggtatacg tttgggtgaa acaatcaatg tacaaatcga acgcggaaaa acgcttatca
    58501 ttcgtttaga tgaaatcgga gaacccgata tcgaagggaa tcgcgtactg ttcttcaatt
    58561 tgaatggaca aagacgtgaa atcactatca aagataattc gatcatcagt acggtccaaa
    58621 cccgattgaa agctgaaccg acaaaccgag aacaaattgg agcaacgatg tctggttctg
    58681 ttttggatgt attagtgaaa aaaggcgata acgttaaaaa aggtgatacg ttgatgatta
    58741 ctgaagcgat gaaaatggaa acggccatcg aagcacgttt tgacggagaa gtcgcacatg
    58801 tatatgtgtc ttcaggagac actatttcgt ctggtgacct tttgattgaa gtgactgaga
    58861 aataataagc gaaaggtggg gaaacatgaa aagactgggt ttgttttggc tgattctgct
    58921 attagttatt gtggcattct acatggaacc agtcttgttt ccgccaaaac ttgaagtgaa
    58981 ggaaaactct gatcaacaaa tatcaaacca aatgacaacg tggaaatacc aggagcttaa
    59041 agcaactggt ttttctactt atatcggaaa accgatcgat cagctggaaa aggattttgg
    59101 ccaagcctat gatcgattaa ctagcggatt tggttttgaa acaagatatt atgaaaataa
    59161 agctagtcat ctcacatttg aagcgaacat ccaagacagc agagtcacaa ctgttaaagt
    59221 actgcaaaca gggcctgctg atatcgcgcc gttttattta gggatgacga tgcaagatct
    59281 tacgaaaatc actactatct atactaattt cacttttgag taccatgata cagaagttgg
    59341 aatcgaacta atggaagagg atatgaatta tcgtccgcta attgcttttg ataacgacac
    59401 ttttgctatt ttattttttg atcaaacaac aaacggccta ttttcagttg tgtatttaga
    59461 taaagatagt cttttgaaac tattgccata tcaagtgttt ggtgaaggcc ttccacatta
    59521 tcaagcagat aaatcggctg attgggaatc aatcaatcgc gttaaagaac gaaaaagtac
    59581 gactttatta aatatgctta gaagagaaga cgaacttccc atatacaatc aaacacttgc
    59641 ctttcaaaac cacagtcagc tgctactaca tgattttttg acaaggcctg aggaaatatt
    59701 gtcagaggaa cgtatggctg attggcaaaa atcgctaggc agtgctcaaa cgacagtgaa
    59761 attctcactg acgaatgatg aattggagaa gttaatcagt aaacaaaaat tagaaaatac
    59821 tagtgggcta tttattcatc cggcgattga cgctactttt tcgtttttat attggtacag
    59881 cgatccttac aaccatgacc gtttcatgga cccgggaacg gattctgtag gcattgcttt
    59941 ttctaaagaa aatatgttag tcttattgca agaagaaaac ggagaaagta gtgatagcag
    60001 tgatcgttaa cgaagagctg tttcaactgg aggatcaatg cctccaagtc gctgaaatga
    60061 taaaaaagag taaaacgatg gcttcttatc ttgaacataa acagtcaatg gaagaatctg
    60121 caaaagtttg ccagttgaaa gaagcgttct tgtctaagaa agaagcattt gaaaatgtag
    60181 cggcatatgg tgagtatgca cctgattatc gtgaaaaaaa acgagctgtt cgaaaagcaa
    60241 aaagacagct tgatatggat gagcatgttg cagctttccg atatagtgaa acacagctgc
    60301 aaagtctgtt ggatcaaatt gtccaagtac ttgccgaaga agtttctaat gaaatcaaag
    60361 tagatgcagg tagcccgttt tttgaaacga aaaaacactt tggatgcgga ggtaattgtc
    60421 atgcaattag acaatagtga atttgaaata acaaaacgac gcgggctgat cgtatgggtc
    60481 tacagcttaa aacaattaaa gaatctgaaa aagttcggct tgttgcatta tgtctcaaga
    60541 agaatgaaat atgtcgtgct ttacttaaat gaagagacat tggaagaaac agaagaacga
    60601 atccgtaaat tacattttgt tcgaaacgta gaacgttctt accgaccgga tatcgaaatg
    60661 aattttgccg aaaaaatagg aaacaaaaca gtattaaaag acgaaggttt cgaaattgaa
    60721 gagctaaata cagaaatccg attagctgaa catctttaaa aatctcttat gagcagtttt
    60781 taaataaaaa ataacttgga taacgatcga tttttgatca ttgtccaagt atttttttgg
    60841 tatactagta ccagtaccat ttctaaggag tgagtagatg cggataatat caggaaatta
    60901 tggcgggcgc cgtttaaaaa gtttagcagg ggcgaacacc agaccaacta cagataaagt
    60961 aaaagaatcc atattcaata tgattggacc atattttgat ggagagacag tattggatct
    61021 gtttgcagga agcggtggtt tagctatcga agcagtatct agaggctgca gtcatgcggt
    61081 atgcgtagac aaaaattacc aagcgctaaa aatcatcaaa gaaaacatcg aaattacgaa
    61141 agaaccagag aaatttacca caataaaact tgatgcggac aaagcaatcg aacggctagc
    61201 agcggagaaa caacaattcg actacttgtt tcttgatccg ccatatgcaa aacaaaaaat
    61261 aatcgatcaa atccataaaa tggaagaact gggacttttt tctaaaaccg cagttatcgt
    61321 atgcgaaacg gataaaacag tcgaattgcc agaaactgtg gctggattcc atcaaataag
    61381 aaaacagatt tatggcataa cggccgtaac tatctataga aaagagggaa atcaatgaaa
    61441 cgtgcattat tcccaggaag ttttgatcct tttacaaaag gtcacttaga tacggtggaa
    61501 cgtgcggcta aattattcga tgaggtagta attggtgttt ttatcaatac ttcaaagaaa
    61561 agtttgtttc ccccagaaga aagaatgaca ttgatcacaa aagctgtttc tcatcttcct
    61621 aatgtaaaag tcatgcatca agaaaatcaa ttgacggtag aaacagcaaa agagattggt
    61681 gcagatgcgt tgatcagagg catacgaagt atcaaagact ttgaatatga acgagagatt
    61741 gctcagatga atcatcatct atatgatgaa ttggaaactg tcttcttatt agcaaaacca
    61801 gaatatagtc acgtgagttc cagtatttta aaagaagtac tgcactttgg cggagatgtg
    61861 agcagctact taccgccagt gatcaacgaa gctttagctg gaaagcgtga atcaaatgaa
    61921 agctaaacaa tggttgaaaa cagtactgat tatcatattt ggaatgttga tcattggatt
    61981 tgtagtagtt cccttgccgt attatgtaga atcaccggga gccacgatcg atctgaaaga
    62041 actaattacg gtcaatgata aggaagataa aggttctggg tctttctctc ttacctctgt
    62101 tggtatcagg caagctacag gtttcttagc tttaaaagct aaaatgaccc cttttgaaga
    62161 attgatcagt gaagaagaat tgactggtgg ggcgacaaat gaagaatata atcaaatgca
    62221 acagtattat atggattctt cccaaaatgc agcaatcgaa caagcgttaa aacttgcaga
    62281 caagccttat aaaatggaat tcaaaggcgt atatgtcatg agtgtagaga aaacatcgaa
    62341 tttctttggc aagctttcag ttggagatac agtaacaaaa gtagacggga aaagctttca
    62401 aagtacagaa gcatttatgg attatgtaaa aagccaaaaa gttggacaaa caatcgaaat
    62461 cagttatttg cataatggtg aagaaaaaac agcttctggt gatctgatcg agttaccaac
    62521 agataaaaag gcaggaatag gtatcacgct tactgaccat accgagatcg aatcagatac
    62581 agatgttcgt attaattctg gctctatcgg tgggccatca gcgggattga tgttcacttt
    62641 agaaatctat gaacaagtca ctggtaaaaa cttgcgacat ggtaagcaaa ttgcaggaac
    62701 tggaacgatc aacagtgaag gagaagttgg ccgaatcgga ggaatcgata aaaaagtggc
    62761 aagtgcggat aaagctggcg tagagatttt ctttgcacca gatgatacga tttcagacga
    62821 tgttaagaaa gaatatcctg aaataaaaac caactacgaa gaagcaaaag ctgccgctaa
    62881 aaaattgaat tcctcaatga aaatcgtacc agtaaaaaca gtacaagaag cgcttgacta
    62941 tctagagaaa atgaaatgaa actaaataat taaagagggg ttgtgacacg agttctgtca
    63001 caacccctct ttttcgtcaa aaatcttcac gataaaaata aaaacaaaag aaaaaattga
    63061 cgtaattaga tgttgaaggg acgattttgc cagagctcac tgtttcttca attttaaatg
    63121 aaaggagtaa tgaatgattt ttactggtca ttctttgaaa aaagaacaat gatagaaaaa
    63181 atcagcaaga aaatactagc ttcacccaaa ttgatcattt ggatgtctat gatagttctt
    63241 atcttttgta tcgcaagtgg ttttttatta tcaaatttgc tattttatag agaaacagaa
    63301 gagactgctg caaaagaaat actttcttcg tttagttcca gtggagaaga tccatcagct
    63361 gaaggaaacg atgtagaaga ggatgctcgt ccaaaggtca tgtacaccga tatcaaagga
    63421 agtgtgaagg agcctggtat ctattccttt tcttcagaag aacgggtcta tgatgtacta
    63481 aagcgtgcag gtggattatt agaagaagcg gatagcgatc gaatcaactt ttctgcgaag
    63541 atagaagatc agcaagtcct atatatccct gctgtaggtg aagagctgcc agaacatctt
    63601 gatcagttgg catcctcgga aggaaaacag cctacagcag attccgagcc tcccaaaata
    63661 aatatcaata cagccactcc atcagaactt cagcagattc ctgggatcgg atcagtcaaa
    63721 gcgcaagaaa tcattcgatt tagagaagaa aacggatcct tccagaaagt ggaagacctg
    63781 caggagattt ctggtatcgg tgaaaagaca gttgaaaaac taaaaaactt cgttacaatt
    63841 aaatagaatg attcaaaatc gactggcacg agcaataaat ggacgctaat ggatactaag
    63901 aataatccaa ttctaagagc ttggcaggac gaggaggtag tatttttgga taggaaatag
    63961 cttgattgtt cgcaaagaaa cattaaaaaa accaatgctg aatgtctatc tccaaaataa
    64021 aatatctggg attcatatta tgaatacggc tgtcagcggc aataacagtc aagctctacg
    64081 agaacgcttt gccaaagatg tacttagcta tacagcagat aaagtattta ttctcattgg
    64141 gacgaatgat ctagcagagc ataaacaact ttctaaagaa acgtaccaaa aaatctgcag
    64201 tggatgatag ttcagctgaa aaaagactat agttctgatc atgctttctt tatcacgcca
    64261 ccagcagtcg atgagacaaa acaaaaatat cggaataaca agttgatcaa agaatatatc
    64321 tctgttctca cagaagtagc aaaacgccat gattgtgaag tatcgatttt tatacggaat
    64381 ttcttgaaca aatggattcg aagaattttt ataatgggct aatggatgac ggacttcatt
    64441 tcggcagtga agggtaggat cttttgtcag acaggatgat tcgttatatt taaagaatat
    64501 tttgtttaag caatgagtac tttttaatca acttgaaaaa acggaggaat ataaaacatg
    64561 ttagaaaaat cttcaaaagc atgcgatacg aataaagaaa aagttactga taagtcacat
    64621 aatgaacgca ttccttggga ccagtacttt atggctcagg cagtactgtt atctttacgt
    64681 agtacgtgta cacgtttaga agtaggagcg acactcgttc gagaaaaaag gatcattgct
    64741 ggcggctata atggagcagt cagtggagat gtccactgca tagatgaagg gtgttacgtc
    64801 gtggatggac actgcttgcg aacgatccat gcagagatga atgcactcct acagtgtgca
    64861 aaacttggta tcccgacgga aggttcagaa atctatgtca cacattttcc atgtttggcc
    64921 tgtacaaagg ctttgttgca agctggtgtg aaagggatca attatctcca tgactatcga
    64981 aatgatcctt atgcccaagc gttgatcgaa caattaggtg tatctgtaca ccaagttgct
    65041 ttagactcac agcatttttc aaaacttcaa aatgaattaa caggaaaaca tgtctagcgt
    65101 tttagaaagg catgttcgaa atcggttgat ttttccggca attacgtcga tgattatctg
    65161 tttgttgtat tacactgaca attggggtta tcgtttgctt cttgccggaa tttttttatg
    65221 gttttcgcta aaagaaggac gttttatcgc gtacttgtgt attttgtgta gcataacggt
    65281 atccatttct tataggctac aagagtattc atttttagag gaaaaaacaa catctgcgca
    65341 aattactgta tttacagata cgattcgtgc aaatggtaat tttttgacaa tggaaggaag
    65401 gttatcagag aataaacaaa agctccagct ttcttatcaa gtaaacacag aagcagaatt
    65461 gaaaacatgg cttaacttcc gaggaaaggg aatagaattg actgctgaag gcgagttcat
    65521 ctctccagca aaaaagcgga atctttataa ttttgaccaa gaagcttact tcaggaacca
    65581 tagaatttct ggacggttcc aaattcaaaa gattgaacag atcaccatgc aaaatcactg
    65641 gcgaaactgg cttcagagaa aaagaggatc ttttatctcc tttgtccaat ctgaatttcc
    65701 gcaaaaaaca agtgtatata ttaattcttt attatggggc tataaagatg caggattcag
    65761 agaaccagaa gaaattttta gggagagtgg tttgctgcat ctgttcagtc tatcgggtct
    65821 acacatccaa ttttatttag ggtggctgta ttatttattt cgacgtatcg gatggccact
    65881 acacgtcagt attattccgt tgagtctact aactatctgt tatgtttcta ttgcaggaag
    65941 ttctatttct gttttgcgtg ctagtttgct gtttttattg cgattgctag tcaaattgct
    66001 gaataagcat tattccacca tggacttatt tgcagttgtc ttttggcttc ttttactatt
    66061 tgatcctcga tttctttttc atgcgggtgg acagcttagt tttttcatga gttttttgtt
    66121 tatcctgatt tcttttgatg gagattggtt gaaaaaaacg gcctctcacg tcctgtttac
    66181 tatgatcacg atgccgttga ttgtatggca tttttatgag tggcctattt taggtagtct
    66241 ttttacatta ctgattgcac cggtattcaa gtttcttttt cttcctggtg tgttatttct
    66301 cacactcttc aatcgctggc tgccggaaac tttacttttg ctgatagaag agggattaat
    66361 cttatttgag cgaataatcg atttttcttc agggtcgtct ctcattatag gacgtttgcc
    66421 ccttgtgcta tctgggatgt atattttcgg attactgatt tgtatggata ggttgaaaga
    66481 acaacctgtg aaacacttgt tttatgttgg tcttttttct tttatttttt tgactttgcc
    66541 atttttacgc ttttcttcaa cgatcgcttt tatcgatgtc ggtcaaggag acagtatctt
    66601 tttgcaaagt ccttttcgaa aagaaacgat tttgatcgat actggaggtc agttacactt
    66661 tgaaagagaa aagtgggcgc aaggtatgat acgtcctcct gctgaaagaa atatcgttcc
    66721 atatctaaaa ggtcagggga taagtgtaat cgataaactc atcttgacac acgacgatac
    66781 ggatcatgtg ggagaattac ccacgttatc tcagcatttt actatcaaaa aaatttatat
    66841 cggttggggc gctggacgaa atgagccatt acaaaggcat ttatctgaag ctcaaaaaaa
    66901 tggtactgag atcattgaaa tcaaacatgg ggacaggatc agcggttatt atgatcttta
    66961 tgtattgact ccttttgaaa agggagaggg aagaaatgaa gattcgattg gtctatggat
    67021 ggaatataac aataggcgct ttttattttt aggagatttg aatcaagcaa tggaaaaaca
    67081 attgcttttg atttatccta atctgaaagc cgatgtagtc aaattaggtc atcacggtag
    67141 ccgtacgtca agtaacacag attttctttc ccatattgaa gcaaaacatg ggattatatc
    67201 ctgcggcgta aataatcgtt atggtcaccc gcacagcgaa gtattggaga cactagaaga
    67261 aaatcagata aaaacatggc gaacggatca acaaggaatg atcacctatc gctggcatcc
    67321 agtttttcat cctcatgggc tagtggaaac aatgatagac tagtaattct catctgctca
    67381 tggtaggata atttttgaaa gtggtgagta atttgagtat acaagcagag ttacaaaaaa
    67441 tacgtaaaga aaaacttgca tcttgttact tgattttagg aacagaaaag tttctccaag
    67501 atcaagttcg gacagaaatt gtcaaaaaaa tccaattaga gggcgaagat gacttaaatt
    67561 ttttaagttt tgacatggaa aatgcaagtt tagatgaggt agtagctgaa gcagagacgt
    67621 tgccgttttt cggagatcaa cggttagtat tcgtagagaa tccttatttt ttaacaggtg
    67681 aaaaagtaaa caatggaatc gaacaaaata ctgatttatt gacggcttat ttgaaagaac
    67741 cattagaatc gactgtatta gtattctttg cgccatatga aaagttggat gaacggaaaa
    67801 aagtaacgaa acaattgaaa aaaacagcaa ttatcatcaa tgtccaacag ttgaatgaaa
    67861 aagaagtacg tcaatatctg atgaatacct tagagaattc ctcaatcaaa atggaacgaa
    67921 gtgcaattga tttattcttg aggttgaccg acttagattt atccaaaatg atgagagagt
    67981 tacagaaatt ggtcctttat ggacaaaatc aacagcaaat cacggtaaga gaagtagaag
    68041 aattagttcc taaaacgtta gaacataata tttttgacat gacccaatat attttaaaag
    68101 ggaagacgga acaagctttg cgtttatacg aagatttagt tatgcaaggg gaagaaacaa
    68161 tcaaaattaa tgcgattttg ctttctcaat tacgtttatt tttacagaca aaatttcttg
    68221 taaagattgg ttatcagcag gcaaatattg ctgaaacatt gaaaatccat ccatatcgtg
    68281 taaaacttgc catgcaggaa gtaaaaaaat tcgatgaaca tttgctggta cagttgtttg
    68341 atcaattagt tgagatggat tatcaaatca aaacgggaca aatcgaaaaa gaattaagtt
    68401 ttcaactatt cgttttgcgc acagggcagt tgttaagaaa tcgataagaa gaaagacgag
    68461 ggcaaagcat gcgggaaggt cgtttgtttt tgtccttttt ttgacaaata aaaaaatcgg
    68521 ccgaagccga ttcttttatt tagcgatttt tttgcttaaa cgagatttgt cgcggttagc
    68581 tttgtttttg tggattagac cttttgattc agccatatca atagctttaa cagcttcttt
    68641 atataacgca tctacgttgt cagcacctga agctacagca tcttcaaatt ttttgattgc
    68701 agtacgcata gcgcttgttt gagatgaatt tttcacatta gcgttttcgc ttgtacgaac
    68761 acgtttgatt gcagattcaa tgtttggcat tagtttcacc tccgataact taagttctta
    68821 cgcgtgttac cgcataattc aacattcatt attataccta aaggcagcgt gctttgcaat
    68881 atgaacatga caagtttttt tccttaacag tcttttatta ggattttttt tcagtaaaaa
    68941 cggatagtac aagctgaaaa aaatcaaaaa gacgctattg tgggaggcaa tagcgtcaaa
    69001 ccaaactaga tttttattta tctatttaaa caaagtttga atgggaatgt ttggtactaa
    69061 gttattatat aacaaagaga accgtttgca aagttttttt tgaaaaggga tacgattccc
    69121 tgttgttctt tgtttttcta ttaagaaact gcagttattt tataaccagt atcctttgat
    69181 cagtaaaggc tattctggtg taccgattat tttaatgtat tcatcgatat tttttatcaa
    69241 catttttttt atgaccaact ggttccgacg gttccatttg ccacttactt ccagcacata
    69301 tttatttagg ggcaaacgta agatatattt tgccaaatag cgatctttaa gcaggccatt
    69361 gatcacacgg tcctcaagct gcaagttgat cccagtcagc atcattggct gttcttgtaa
    69421 aacgtacgta tcaattaaaa gaccttcaaa agtttccaca agcgtcttcc tttcttttca
    69481 aagtaacctg aaaacaggca ctaattcttc cattttgata ttcgttgaag tggatgcgat
    69541 cattctttct tttgtccact gtaatctagt ataacaaaaa agagcagaag agccaaaaag
    69601 aaaaaagagc atcgaattct ataggaagtc tcattggcac ggcctaaatt attacaaaac
    69661 attgattaaa aaaagcccct gcatcgcagg agcatcccct atatcgtttt aataggtcaa
    69721 tcaaaaagca gtatcaaaca cattttgtat caaatcagga aataatagat aagctaagcc
    69781 cagaatcaaa agtaccgcta gaagaaacca gccgattgtt cctatgaaat actttaattt
    69841 ttcgaacatt gtatcacctc gttgtcagta tatcacggat cagtaaattg ggggtgttaa
    69901 gaatctccta aaatagacaa aatttgttct attttcctaa agcaaatact tgtaagatac
    69961 agtaagtgat accagaagca atgaccggtc cggcggcgat tcctcttaag aagacaacac
    70021 ctaaaatcgt gccgaaaaca agtgcaacag tgatttgtgg atcagaagca atcaatccta
    70081 cacccttgga agataagaca gccactagcc caccgcaaaa aacagctacc cagccaatag
    70141 gggatttaaa ggcttctaat agatcacgta gaccgatttc tcctgttgcg atagggatta
    70201 gaatagccgc tgtgatgatc gtcaccccaa tattgattcc tttgccttga agccaaggga
    70261 aaaaggaacg ggaggctggt atgagcttca taatcaaaac aaaagctgcc gctatcatca
    70321 gactttgatt tttagctaaa attgctacaa gtaaaataat tcctaaaaaa agccaacttt
    70381 ccatgaaata tattcctcac tttcttcgaa acaatcataa catgttttta gaaagttaca
    70441 aaagtaggaa ttcatccttc aagacctatt cataagctgt ttttttcata aaataaacaa
    70501 aatcataaaa aaacactttg cttatttgac agaagacagc cgcgctttgt atagtggaca
    70561 agacttacat ttttttgaag aatcagaaag atcaggtaaa ggtcttagta aacgcaagcc
    70621 attcagaata accaatattg tgcttccttc gtgtcctaca actccaagag gtagatttaa
    70681 aaattgcagg aagttagaag caatcaacaa gagaataaca agaatcgaaa aaataatatt
    70741 ttgtttgatg atccgccgca tttttaaaga tagccgatga ctgtagacaa atttatttaa
    70801 gtcgcttttc attaagacga tgtctgcgat atccatagcg gcatctgtcc cttgccccat
    70861 agcgacacct aaagaagcgg ctgctaaagc aggcgcatca ttgactccat caccaaccat
    70921 cgcatttact tggtaaagtt cattctcttt tttgactagt tgtgtttttt cttccggagt
    70981 acagcttgca tagtaatgtt caatccctac ttgttcagca atggcctttg ctgtgccaga
    71041 atggtcgccg gttatcatac ttgtatggat gttttgggat ttaaagtagg aaattacttg
    71101 ttgggtattt gcttttggaa tatctagcag acctaaaaca gcaattaact gctgatcttt
    71161 agaaagataa atcacggttt tgccttgatt ttccaaacgt tcgatcattt ccgcaatctc
    71221 tttgctgatt atcatcgtct caggatcata tgcgtgcttg ccaactttcc attgcgcacc
    71281 taaataggta gtttgtaatc caaaaccagt aatttcttct attggtaagt attggatttc
    71341 ttcaggtatt gtgcaatcga aatattgcaa aatcgcatga gcaagtggat gcgtcgtttt
    71401 ttgttccata gcaacaagaa gttgttgtgc caaatccttt tcatcaaaga aaaagctgtc
    71461 agttacaact ggttttcctt tagtgagcgt tcctgtttta tcaaaggcaa tcgcttttaa
    71521 ttcagaaagc ttttctaaat ggataccgcc tttgattaaa atcccatttt ttgcagaatt
    71581 tgaaagagca gctagagaag ctggtgtagc agatgcgaca agcgcacaag gagaagccac
    71641 aactaataat accattcctc gataaaagct ttcatcccaa ctccaaccta ggaaaaagtg
    71701 ggggagaagg atcattagag gaacagcaag caaaatcaat tttacataat tattttcgat
    71761 tcgttcgata aatgaagctg tttgagttgg cgtattctgc gcttcttcta ctaattggat
    71821 aatcttagca aagacagttt cggaactcgt ttttgttact cgaatcgtga tgggatttcc
    71881 ttgattcagc gttcctgcat acatctcatc gcctaaatat ttttctacag gcacagattc
    71941 accgcttaat gcagactcat cgatcgttgc ctgtgtgctt tctaaaaagc catctatcgg
    72001 aacagctgct cctttagcga caaataccaa gttatccacc tttagttgag aaacatctac
    72061 ttcttgcatt tgtccatttt cttttaataa ttgggctttt tgtggttgaa gattcatcaa
    72121 agcagtaatt tctttttgac ttttattagt tgtgtattcc tctaatgcac cgcttaaaca
    72181 aaagatgaat gtcaacattg ctccttcaaa gtaattaccg atcaaacagg caccaatagc
    72241 agctaaagcc atcaataaat ccacattcag tgtccgatca ttccacaatt ctttcaagcc
    72301 ttccgatgtt tgtttatatc caccgatgat aatggcactg atataaaaaa gtggggtgat
    72361 ggtaagagag aatcggtcga agataaaacc gaagatcata aataaaaggc agccgattgt
    72421 tgctgcaaaa gcttttttct gattagtcat cgttattaga cctccttgtc aaaacaagga
    72481 tagcataata aaaatgtaat tgataataat aacagct
//
LOCUS       DAJSAW010000006        71080 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJSAW010000006 DAJSAW010000000
VERSION     DAJSAW010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.006"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            161..391
                     /locus_tag="ONT64_000467"
     CDS             161..391
                     /locus_tag="ONT64_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCR2301908.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="ONT64_000468"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="ONT64_000468"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR2301909.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="ONT64_000469"
     CDS             1802..1978
                     /locus_tag="ONT64_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR2301910.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="ONT64_000470"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="ONT64_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR2301911.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2593..3060
                     /locus_tag="ONT64_000471"
     CDS             2593..3060
                     /locus_tag="ONT64_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2301912.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3145..4029
                     /locus_tag="ONT64_000472"
     CDS             3145..4029
                     /locus_tag="ONT64_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2301913.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4143..4379
                     /locus_tag="ONT64_000473"
     CDS             4143..4379
                     /locus_tag="ONT64_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HCR2301914.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4467..4547
                     /locus_tag="ONT64_000474"
     tRNA            4467..4547
                     /locus_tag="ONT64_000474"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4501..4503,aa:Tyr,seq:gta)
     gene            4554..4625
                     /locus_tag="ONT64_000475"
     tRNA            4554..4625
                     /locus_tag="ONT64_000475"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4586..4588,aa:Gln,seq:ttg)
     gene            4750..4920
                     /locus_tag="ONT64_000476"
     CDS             4750..4920
                     /locus_tag="ONT64_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301915.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5140..5847
                     /locus_tag="ONT64_000477"
     CDS             5140..5847
                     /locus_tag="ONT64_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2301916.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5907..6551)
                     /locus_tag="ONT64_000478"
     CDS             complement(5907..6551)
                     /locus_tag="ONT64_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCR2301917.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6678..6965
                     /locus_tag="ONT64_000479"
     CDS             6678..6965
                     /locus_tag="ONT64_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301918.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7073..7393
                     /locus_tag="ONT64_000480"
     CDS             7073..7393
                     /locus_tag="ONT64_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2301919.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7474..8787
                     /locus_tag="ONT64_000481"
     CDS             7474..8787
                     /locus_tag="ONT64_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR2301920.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8784..9503
                     /locus_tag="ONT64_000482"
     CDS             8784..9503
                     /locus_tag="ONT64_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCR2301921.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9631..10182
                     /gene="raiA"
                     /locus_tag="ONT64_000483"
     CDS             9631..10182
                     /gene="raiA"
                     /locus_tag="ONT64_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR2301922.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10404..12938
                     /gene="secA"
                     /locus_tag="ONT64_000484"
     CDS             10404..12938
                     /gene="secA"
                     /locus_tag="ONT64_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR2301923.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13064..14165
                     /gene="prfB"
                     /locus_tag="ONT64_000485"
     CDS             join(13064..13135,13137..14165)
                     /gene="prfB"
                     /locus_tag="ONT64_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR2301924.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14270..14956
                     /gene="ftsE"
                     /locus_tag="ONT64_000486"
     CDS             14270..14956
                     /gene="ftsE"
                     /locus_tag="ONT64_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR2301925.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14940..15833
                     /locus_tag="ONT64_000487"
     CDS             14940..15833
                     /locus_tag="ONT64_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2301926.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15985..17433
                     /gene="cls"
                     /locus_tag="ONT64_000488"
     CDS             15985..17433
                     /gene="cls"
                     /locus_tag="ONT64_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR2301927.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17770..18627
                     /locus_tag="ONT64_000489"
     CDS             17770..18627
                     /locus_tag="ONT64_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR2301928.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18742..19662
                     /gene="pstC"
                     /locus_tag="ONT64_000490"
     CDS             18742..19662
                     /gene="pstC"
                     /locus_tag="ONT64_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCR2301929.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19662..20546
                     /gene="pstA"
                     /locus_tag="ONT64_000491"
     CDS             19662..20546
                     /gene="pstA"
                     /locus_tag="ONT64_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR2301930.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20561..21367
                     /gene="pstB"
                     /locus_tag="ONT64_000492"
     CDS             20561..21367
                     /gene="pstB"
                     /locus_tag="ONT64_000492"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCR2301931.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21383..22141
                     /locus_tag="ONT64_000493"
     CDS             21383..22141
                     /locus_tag="ONT64_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCR2301932.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22154..22831
                     /gene="phoU"
                     /locus_tag="ONT64_000494"
     CDS             22154..22831
                     /gene="phoU"
                     /locus_tag="ONT64_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR2301933.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23069..24100
                     /locus_tag="ONT64_000495"
     CDS             23069..24100
                     /locus_tag="ONT64_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCR2301934.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24097..24864
                     /locus_tag="ONT64_000496"
     CDS             24097..24864
                     /locus_tag="ONT64_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCR2301935.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24964..26532
                     /gene="liaX"
                     /locus_tag="ONT64_000497"
     CDS             24964..26532
                     /gene="liaX"
                     /locus_tag="ONT64_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HCR2301936.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26829..27149
                     /locus_tag="ONT64_000498"
     CDS             26829..27149
                     /locus_tag="ONT64_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HCR2301937.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27149..27499
                     /locus_tag="ONT64_000499"
     CDS             27149..27499
                     /locus_tag="ONT64_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR2301938.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27666..28604
                     /locus_tag="ONT64_000500"
     CDS             27666..28604
                     /locus_tag="ONT64_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCR2301939.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28619..29449
                     /locus_tag="ONT64_000501"
     CDS             28619..29449
                     /locus_tag="ONT64_000501"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR2301940.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29493..30518
                     /locus_tag="ONT64_000502"
     CDS             29493..30518
                     /locus_tag="ONT64_000502"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR2301941.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30533..31471
                     /gene="galU"
                     /locus_tag="ONT64_000503"
     CDS             30533..31471
                     /gene="galU"
                     /locus_tag="ONT64_000503"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR2301942.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31596..32036
                     /locus_tag="ONT64_000504"
     CDS             31596..32036
                     /locus_tag="ONT64_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCR2301943.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32039..32641
                     /locus_tag="ONT64_000505"
     CDS             32039..32641
                     /locus_tag="ONT64_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCR2301944.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32700..33806)
                     /locus_tag="ONT64_000506"
     CDS             complement(32700..33806)
                     /locus_tag="ONT64_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCR2301945.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34016..35017
                     /gene="ccpA"
                     /locus_tag="ONT64_000507"
     CDS             34016..35017
                     /gene="ccpA"
                     /locus_tag="ONT64_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCR2301946.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35354..37699)
                     /locus_tag="ONT64_000508"
     CDS             complement(35354..37699)
                     /locus_tag="ONT64_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCR2301947.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            37978..38880
                     /locus_tag="ONT64_000509"
     CDS             37978..38880
                     /locus_tag="ONT64_000509"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR2301948.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            39093..40868
                     /locus_tag="ONT64_000510"
     CDS             39093..40868
                     /locus_tag="ONT64_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HCR2301949.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40934..42520)
                     /locus_tag="ONT64_000511"
     CDS             complement(40934..42520)
                     /locus_tag="ONT64_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR2301950.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            42771..42995
                     /locus_tag="ONT64_000512"
     CDS             42771..42995
                     /locus_tag="ONT64_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCR2301951.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43137..44888
                     /locus_tag="ONT64_000513"
     CDS             43137..44888
                     /locus_tag="ONT64_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2301952.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44888..46672
                     /locus_tag="ONT64_000514"
     CDS             44888..46672
                     /locus_tag="ONT64_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2301953.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            46998..47849
                     /locus_tag="ONT64_000515"
     CDS             46998..47849
                     /locus_tag="ONT64_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2301954.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47903..49654
                     /locus_tag="ONT64_000516"
     CDS             47903..49654
                     /locus_tag="ONT64_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2301955.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            49658..49828
                     /locus_tag="ONT64_000517"
     CDS             49658..49828
                     /locus_tag="ONT64_000517"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2301956.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(49868..50770)
                     /locus_tag="ONT64_000518"
     CDS             complement(49868..50770)
                     /locus_tag="ONT64_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2301957.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            51001..52404
                     /locus_tag="ONT64_000519"
     CDS             51001..52404
                     /locus_tag="ONT64_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR2301958.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52404..53837
                     /locus_tag="ONT64_000520"
     CDS             52404..53837
                     /locus_tag="ONT64_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR2301959.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            53982..54908
                     /gene="trxB"
                     /locus_tag="ONT64_000521"
     CDS             53982..54908
                     /gene="trxB"
                     /locus_tag="ONT64_000521"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR2301960.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55122..56849
                     /locus_tag="ONT64_000522"
     CDS             55122..56849
                     /locus_tag="ONT64_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCR2301961.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56864..57214
                     /locus_tag="ONT64_000523"
     CDS             56864..57214
                     /locus_tag="ONT64_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR2301962.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(57306..58166)
                     /locus_tag="ONT64_000524"
     CDS             complement(57306..58166)
                     /locus_tag="ONT64_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCR2301963.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58258..58686
                     /locus_tag="ONT64_000525"
     CDS             58258..58686
                     /locus_tag="ONT64_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR2301964.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58848..59024
                     /locus_tag="ONT64_000526"
     CDS             58848..59024
                     /locus_tag="ONT64_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR2301965.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            59051..59497
                     /locus_tag="ONT64_000527"
     CDS             59051..59497
                     /locus_tag="ONT64_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HCR2301966.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(59651..61030)
                     /gene="brnQ"
                     /locus_tag="ONT64_000528"
     CDS             complement(59651..61030)
                     /gene="brnQ"
                     /locus_tag="ONT64_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCR2301967.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            61736..62707
                     /locus_tag="ONT64_000529"
     CDS             61736..62707
                     /locus_tag="ONT64_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR2301968.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            62731..64923
                     /locus_tag="ONT64_000530"
     CDS             62731..64923
                     /locus_tag="ONT64_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HCR2301969.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            64940..65416
                     /gene="ybeY"
                     /locus_tag="ONT64_000531"
     CDS             64940..65416
                     /gene="ybeY"
                     /locus_tag="ONT64_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR2301970.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            65400..65795
                     /locus_tag="ONT64_000532"
     CDS             65400..65795
                     /locus_tag="ONT64_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HCR2301971.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            65810..66709
                     /gene="era"
                     /locus_tag="ONT64_000533"
     CDS             65810..66709
                     /gene="era"
                     /locus_tag="ONT64_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR2301972.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            66852..67664
                     /gene="recO"
                     /locus_tag="ONT64_000534"
     CDS             66852..67664
                     /gene="recO"
                     /locus_tag="ONT64_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR2301973.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            68048..68965
                     /gene="glyQ"
                     /locus_tag="ONT64_000535"
     CDS             68048..68965
                     /gene="glyQ"
                     /locus_tag="ONT64_000535"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR2301974.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            68967..71042
                     /locus_tag="ONT64_000536"
     CDS             68967..71042
                     /locus_tag="ONT64_000536"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR2301975.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        23266 a        12442 c        15206 g        20166 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact
     2521 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2581 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2641 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2701 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2761 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2821 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2881 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2941 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     3001 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     3061 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3121 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3181 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3241 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3301 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3361 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3421 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3481 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3541 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3601 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3661 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3721 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3781 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3841 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3901 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3961 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     4021 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     4081 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4141 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4201 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4261 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4321 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4381 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4441 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4501 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4561 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4621 cccagtatac cacaatcgta agctctcgtt agttcgagag ctcttttttt tgctttgcta
     4681 ccttgttcct tgaagattct gctgatttaa gatagagtta agttaactac aagaattgga
     4741 gggatccaca tgaaatggaa agaaaaagta gacgaagcag ctgaaaagtt atatgatttg
     4801 gtcaaatcag aaaaatacaa tattgaagtg aatattccga aaaaaggcgg aaaagcggtt
     4861 caggtaaaat caaaacgccc aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa
     4921 taataacaaa agatccataa gtcggcattt tcatcgattt tagggtcttt tgttattttc
     4981 taggttgaac atatctaaaa aattgtttct gacaatagga gtaggaagaa cgatgtgtgt
     5041 ctaaatcctc tgttgatgga tagaaacctt tatctttttc agttgtgaat caggtatata
     5101 ccagttataa tagaagaaac gaaaggacgg gtgtacgtta tgtcgaatca attacctgta
     5161 tatatacaga ttcatgacca attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga
     5221 gaccgtcttc cttcagaaag agaattagca gtgaaattca atgtcagccg tatgacgttg
     5281 agacaagcaa ttcaaaactt ggcagatgaa ggaatcttag aaagaaagat cggttcgggt
     5341 acatatgttg cgcgagaaaa agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa
     5401 attatggaat cgcaaaatcg agtaccgtct agtcggacaa tctcttattt cttgacttcg
     5461 ccaagctcaa gtgaaatgga aaaactaaac ttgagtaaag atgacacgat cttgcgaatg
     5521 gaacgtattc gtttcgcaga tgatgtccct atttgttttg aagttgccag tgtgccacaa
     5581 aagctgatcg atggctacag caaagcagag attactgaat cgttgtatcg cacgctagaa
     5641 gaaaaaggcg ggaaaaagat cggtccagcg aatcaaacga ttactgctgt gttggcttct
     5701 gagaaaattg ctgactattt agaggtaaaa cgaggagatg cagttcttcg tttgcgccag
     5761 atttcttatt ttgaagacgg tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat
     5821 cgttttgagt tttatttgga aaaataaaaa acaggtgatc cctgattcct tacttagaaa
     5881 taaaagagaa tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa
     5941 gaaacaggac cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta
     6001 aagtggtcag cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg
     6061 atttctttta taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga
     6121 tacgtgatca tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac
     6181 aatgcttgag agacagcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt
     6241 cctccaaaat atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact
     6301 tccaacatcg gtacgccagc cgttccactt ggttctccat catcgctgct tcgctggatt
     6361 tcattttttt ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt
     6421 tttatttctt gaatgaaact ttttgcttgg tcctctgatt ctattcgtct aagagaacag
     6481 ataaagcgtg attttttgat ctcaatctca ctttgtccat tttcttttat tgtacgatag
     6541 cgctcgatca tcttgtcacc tctttttttc attttcttta ttcagtataa ataaagttct
     6601 aggttgtttc aacactgtaa cggttgcgca atcgctgtag tatgcgataa taaagacaaa
     6661 ggtaggtgga tctggggatg caggaacgaa tgaaaaagta cgatgcaatt acgcattatt
     6721 tgaagaacaa tggcggctct caagtaacgt tgacctttac ccaatttgac gaactgctgt
     6781 ttcccagtaa cggacttccc aaaacagcaa gggaaagtac agattggtgg gcaaatgatt
     6841 ataaacaccc tgaaaaaggt gcctatggct ggataaatgc aggctatgaa gtagttgtca
     6901 tcaatttgga caaagaatat gtcgtgttca ataaattagt gaaatctagt tggctatttg
     6961 attaaataaa ccgcgtttag aaaacgttga caaatttctt tctttttata taatagagta
     7021 acagacgcga agaaaacgtt tgcgtagttt gttttaaaaa ggagagaaaa gtatggctaa
     7081 aaaaacaatc atgcttgtat gttcggctgg tatgagcaca agtttactag taacaaaaat
     7141 gcaaaaagct gctgaagcaa aaggaatcga ctcggatatt ttcgctgtgt ccgcttctga
     7201 cgcagataac aatcttgcaa ataaagatgt agatgtactg ttattaggac ctcaagttcg
     7261 ctttatgaaa gctgattttg aaaaacgtct ggaaccaaaa ggcattcctt tagacgtcat
     7321 caatatggct gattatggta tgatgaatgg cgaaaaagtg ttagatcaag caatcacgct
     7381 cataggtgaa taaatagaac agtataacta tcgaacaagc gatccaaaaa atagatcgct
     7441 tgttttttcg tatatttttt tagggcgaat aatatgaatc atttttgggg tagaaggctt
     7501 ctgacgagag atattaaagc aatagactgt gaggaggtaa atttaccaaa ttatgagaag
     7561 atcgcagcag tggaacgttt gggcaactgg attctttgtc atcgctgtgg aatgaggacg
     7621 tccgtcattg atggacagct tgcagattac ggttattttt gtacacattg tctatattta
     7681 gggcgatgcg acactcaaca agatttatat ttgtttgatc agcctgaagc aaaacctcga
     7741 gaagtcgtct ttttttggac aggacagctt actgaaaaac aaacggaaat cgcaaagaga
     7801 atactttgcc attcagaaaa aagacatcat cttatttggg ctgtaacagg tgcagggaaa
     7861 acagagatgc tttatcctat tttagtggaa actttaaaag ctggaggaag agtagcgatt
     7921 tgtacaccgc gagtcgatgt ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca
     7981 gaagaaaaaa tcacgttgct ccatgggaat acagcaacgg cttatacctt tacttctttt
     8041 gttatctgta cgattcatca gctataccgt ttttatcggt cctttgattt aatagtgatc
     8101 gatgagatcg atgcgtttcc ttataacggg gatgcaggat tagctcatgc tgtgcaaaca
     8161 gcgattaaac cagacggacg attcatctac ttaagtgcca cacctgataa aaagttacta
     8221 aaggaaatca agcagacgtt tgacatccac aaacttgctt tgcgctttca tcggcgttta
     8281 cttcctgaac ccgtcttggt tttcatgaat aattggcgac aaaaatgcaa gaacaagaag
     8341 aagataaaat ctttggtgcg gatcattcat caattaatta aagaaaacca tatcctttta
     8401 ttttgtccaa gtatcacgat gatgcatcgt ttgaaagcca cacttcaaga ggctcttccg
     8461 agatatgaga tcacagatgt ttcttcaaga gacaatcagc gggcggaaaa agtccagaaa
     8521 atgcgagaag caaaatatga catactgtta accacgatga tccttgaacg tggagtcaca
     8581 tttgaaagaa tatccgttat cgttttagga gcggatcatc cggtttttac taaatcttct
     8641 ttggtacaaa tcgcaggaag agccgatcgt aaagggcctt atacgaacag tcaagtatac
     8701 tttttctatg aagaaaaaac tgcagctatt cgaaaagctt gtcaggaaat caaagaaatg
     8761 aatgaagaag ggaagaagat gctgtgaggt gcagttgctg cagtaaagat atcgtccaaa
     8821 atttaacatt cagcgagatt attggtgttt cgaaatacag attacctcag acttgttcag
     8881 tttgtcgcgc acaatttcat ttattggaaa caagaggctg ttctggctgt caaaaaccaa
     8941 tccatttcaa tctagagagt acgaaaaaga aagtaaagtc tattcccgtt tactgcagtg
     9001 actgtcaaga atggaagaag aaatatccga cctatgattt tcagcaccgt gccttttttg
     9061 tgtacgatga aaatatgaag gcctggctca atcaatacaa gtttatggga gatatccgac
     9121 tagcaggcac ctttgcatat aaatggacgc agataaaaaa agaatacccg acatacatct
     9181 attgtccaat acctttatct gaaaaaaggc gaacagaaag aggattcaat caagtaagcg
     9241 agatgttgaa agcagcaaga attccttttc agatgctact caaaagagag aagcatctgc
     9301 tggctcaagc aaaaaacacg cgaaaggaaa gattggagat gcctcaacct tttgaacttg
     9361 cagtcacctt ttctaagata caaaataaaa aaatattgat tattgatgat gtctatacaa
     9421 caggacagac tcttttccac gcagcagatt gtttgcaggt cgcacagccg aaaatcatcc
     9481 gaaccatttc tttagcaaga tgaattagat gtggaaattt gatagcgatt taatttgcaa
     9541 ataacctaga gttcgatata atagaattaa gaagagggaa agagtggtcc ttctccttct
     9601 tgattgtggt agacgaaagg ggtaattttt atgtttagat ataatgtacg cggcgaaaat
     9661 atcgaagtaa ctgaagcgat tcgcgactac gttgagaaaa aagtaggcaa actagaacgc
     9721 tatttcagtg acgcacccga tgcaacggcg catgtgaact taaaagttta tacagaaaaa
     9781 acagcaaaag tggaagtaac gatcccatta ccttatcttg tattacgtgc cgaagaaact
     9841 tctccagatt tatacgcaag tatcgatttg gttgttgaca aattggaacg ccaaatccgc
     9901 aaattcaaaa caaaaattaa tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct
     9961 actgctttat tcaatgaaga ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt
    10021 acgaaacgcc tttcattgaa accaatggat agtgaagaag cagtcttaca aatgaacatg
    10081 ctgggacata acttctttat ttttgaagat gcagaaacaa atggtacaag tatcgtttac
    10141 cgccgtaaag atggaaaata cggactgatt gaaacagact gatcagcctt acttaaataa
    10201 caacgaaaac ttgcagcagc tactgacagc tgccgcaagt ttttttgagt ttcctcctct
    10261 tttagatgaa atatgaataa attatgaagt tcttacttta atacgcttaa gatatttcaa
    10321 ttatctataa ctaatgataa aatgaaaagt aaagtctaca tagtctggat aatacacaga
    10381 aggaaaagga gattacacaa cgcatggcca atttgattcg ttcacttatc gaaaacgata
    10441 agaaagaact aaaacgttta gatagcattg caaaaaaagt tgaagcatac gctgaccaaa
    10501 tggcagcatt aactgatgaa caattacagg caaaaactgc tgaattcaag ggcagatacc
    10561 aaaaaggaga aacattagat caattacttc cggaagcttt tgcagtcgtc cgagaagcag
    10621 caaaacgtgt attggggtta tatccacacc acgtacaatt gatgggtgga atcgtgcttc
    10681 acgatggaaa tatccctgaa atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc
    10741 cagtatatct aaatgctctg actggcgaag gggtacacgt agttaccgtc aatgaatatt
    10801 tagcaacccg tgactcgacg gaaatgggcg aactttataa tttcttagga ttaacagtgg
    10861 ggttgaatat caactcaaaa tcttcggatg aaaaacgcga ggcttataat tgtgatatca
    10921 cttatagtac gaataatgaa ttaggatttg actacttgag agacaatatg gtcgtttacc
    10981 gccatcaaat ggtacaacgt ccgctgaatt atgccattgt cgatgaagtt gactcaatct
    11041 tgattgatga agcgagaacg ccgctgatca tttcagggca agcagaaaaa tccacagctt
    11101 tatatacaag agcagacaac ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg
    11161 atgtacaatc aaaaacaatc agtttaaccg aagcagggat cgaaaaagca gaagaaacat
    11221 tcggtttgga taacttatac gacattgaaa atactgcatt gacccatcac ttggatcaag
    11281 cattgagagc caactatatc atgcttttag atatcgatta tgttgtccaa gacggaaaag
    11341 tactgattgt tgaccagttc actggtcgga tcatggatgg acgccgttat tcagatggac
    11401 ttcaccaagc aatcgaagct aaagaaggcg tagagatcga agacgaaacg aaaaccatgg
    11461 cgacgatcac tttccagaac tatttccgta tgtacaagaa acttgcggga atgactggta
    11521 cagctaaaac agaagaagaa gaattcagag aaatttataa catccaagta taccagattc
    11581 cgacaaatcg tccggttatc cgtgatgatc gtccagattt gctttaccca acattacaaa
    11641 gcaagttcca cgctgtcgtg caagatatca aagatcgcta ccgcaaaggt caacctgtgt
    11701 tagtcggaac tgttgcggtt gaaacatctg agttattatc agatatgctg aatcaagaac
    11761 gtattcctca tgaagtgctg aatgcgaaaa atcactttaa agaagcagag atcatcatga
    11821 atgctggtca aaaaggtgct gtgacgattg ctaccaacat ggctggtcgg ggaacagata
    11881 tcaagttagg acttggggta agagaacttg gtggtctagc agttatcggg acagaacgac
    11941 atgaatctcg tcgtatcgat aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg
    12001 gtatgtcaca gttctacttg tcattggaag atgatttgat gaaacgattc ggttcagaac
    12061 gaatcaaatc tttcttagat cggatgaaga tcggtgagga agatgctgtt atccaaagca
    12121 agatgctgac gaaacaagta gaatctgcac aaaaacgtgt agaaggaaat aactatgaca
    12181 cacgtaaaaa cgtcttacaa tacgatgatg tgatgcgtga acaacgtgaa gtaatctata
    12241 aacagcgaca agaagtaatc atggaagaaa aaagcttatc caagccacta atgaacatgg
    12301 tcaaacggac aatcagtcgt gtagtagatg cgcatacaca attagagaaa gaaaattgga
    12361 atttggaaag tatcatcgat tttgccggca gtactcttgt ccatgaagat agtattactc
    12421 ttgcagatat cgaaggaaaa acagctcagg agatcaaaga gtatctaaac gaacgagcac
    12481 aagaagtatt taataccaaa gcggctcaac taaatggtcc agagcaactg ttggaatttg
    12541 aaaaagtcgt gatcttgcgt gttgtggact ctaaatggac agaccatatt gatgcaatgg
    12601 atcaattacg tcagtcaatc ggcttacgtg cttatggaca gaacaatcca ttagtcgaat
    12661 atcaaacaga aggctataag atgtttgaag atatggtagg cgccatcgag tatgaagtca
    12721 ctcgaatctt tatgaaagct gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag
    12781 gaacagcttc tcactcagaa gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa
    12841 aacagccaat ccatgtgaac aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga
    12901 aaaaatataa gaactgtcat ggaaaaaatg caaactaatt tctgacagca ggattggtga
    12961 gacaaattct ttttttaaga attcgtctca ccttctttct gttatttgct gattctgtta
    13021 gactagatat aatgaaaaaa gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat
    13081 tcgaaatctc ctgacagaaa tgcaggaaaa ggtcacaagc ttcaggaggt ctctttgact
    13141 tagaccagct ggaagaagac attgcggaag cagaagcaaa aatggctgaa cctggttttt
    13201 gggatgatag cgaagctgct caaaaagtga tcagtgaaaa caatgcaaat aaagaaactt
    13261 atgatcaatt ccattctctt gctgaagaac tggaagaaat cgaattgatg gtggagatgt
    13321 tgcaggaaga acctgatgca gccatgcaag aagaagcaga aacacgtatc aatgcgctag
    13381 atgaaaaaat gaagacatat gaattatcta tgttgttaga tggtccttat gatcgaaaca
    13441 acgccatcgt tgaattgcat ccaggggctg gcggaacgga gtcccaagat tggggcagta
    13501 tgcttttacg tatgtataca cgctgggcag agcagcatgg gtatcaagta gagacactag
    13561 attatcaagc aggtgatgaa gcaggaatca agagtgtgac cctactgatc aaaggacaca
    13621 acgcatttgg ttatctgaaa tcagaaaaag gtgttcatcg ccttgtacgt atctcgcctt
    13681 ttgactcagc gaaacgacgt catacttctt tttgttcggt agatgtgatg ccagagcttg
    13741 atgaaacgat cgatatagaa atcaatccag atgatttgaa aatcgatacg taccgagcaa
    13801 gtggtgcagg tggtcagcac atcaataaaa cagaatctgc tgtacggatc actcacttgc
    13861 ctacaggaac agtagtagct agtcaggcac aacgttcaca attgaaaaac cgtgagcaag
    13921 cgatggggat gttaaaagct aagctttatc aattagaagt ggaaaagaaa gaacaagaag
    13981 cagctgcact tcgaggagaa caaaaagaaa tcggctgggg ttcacagatc cgttcttatg
    14041 ttttccatcc atattctatg gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc
    14101 aagcagttat ggacggtgac cttgatttat ttattgacgg ctatttaaaa atgcatctac
    14161 aataagaacc cattgttaaa ttgaaataaa attgtaagat tttgcaagca tgttgaaaca
    14221 ttgccatgct ataatagcta aagactgaaa aagatatgga gagaacgcca tgattgaaat
    14281 gaaggatgta atgaagaagt actcaaatgg tacgaccgct atccgcaatc tctcaattgt
    14341 catcgaacaa ggagagtttg tctatgtggt tggtccgtct ggagccggta aatctacatt
    14401 catcaaattg atgtatcgag aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca
    14461 tgatctaata gcaatcaaaa accgcgaagt tccttatcta cgcagagaaa tcggcgtggt
    14521 gttccaagac tataaattat taccaagaaa aacagtatat gaaaatgttg cgtacgctat
    14581 gcaagttatc ggcagaaaac ctcgggatat caaaaaacga gtaatggaag tgctggacct
    14641 tgttggtttg aaacacaaag ttcgagtctt tcctagcgaa ttatccggtg gggaacagca
    14701 acgtgtctct attgctcggg caatagtgaa tacgcctaaa gtacttatcg cggatgaacc
    14761 gacaggaaat ctggatccag aaaactcatg ggaaatcatg aaattattag atcggatcaa
    14821 tgcccaagga actactgtcg tgatggctac acataacagt acgatcgtaa atacgattcg
    14881 gcaccgagta attgctatcg aaaacggccg cattattcga gatcaagcgg aaggggaata
    14941 tggatacgat gattagaacc tttttccgcc atttattaga aagtatcaag agtttgaaac
    15001 gtaacggctg gatgacagtt gcttcagtca gtgcggtaac gatcacattg actttagtcg
    15061 gtgtattcat ggcagttatc atgaacgcaa ctaaattagc tcaagatata gaaggaaacg
    15121 ttgatgtctc tgtattcgta gatatcggta caaaagacga agagatgaaa aagctagaaa
    15181 aagaattgaa tgacttagat catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat
    15241 tgaaaaaaat ccaaaacgaa atgggtgatt cctggaaatt gtttgaaggt gacaataacc
    15301 cgttatatga tgtgtatgta gtaagtgcta ctgatccttc ttatacaaaa caagtagcag
    15361 aagaagcagc agatttgaag aatgtttctc gttctgatta cggtggagct tcctcagatc
    15421 ggattttcca aatcgctggt gcagttcgga catggggatt agttgcagcg gcattgctgc
    15481 tgttcgtagc gatgtttctc atttcgaaca cgattcggat cacgattttg tctagacagc
    15541 gtgaaattca aatcatgcgt ttagtcggtg caaaaaatgg atatattcgc tggccgtttt
    15601 tccttgaagg tggctggatc ggtctgatcg gtgcgattct tccgattttg atcatcacat
    15661 ttggttatgc atggtttttc caattgatca atccatcatt attacgttcc cattattcat
    15721 tgattcatcc acaaaatatc gtttggaaaa tcaatctttt aatggtcggc attggtgtga
    15781 tcattggttc acttggctcg atcatttcta tgagacgttt cttgaaattc taataccaca
    15841 atataaataa aatgagaaag ttggacagtt tcgttccagc tttctttttt tattggctag
    15901 gagaatagaa ggaagcggct tttttccctt tactttaaaa aacaacttgg gtattcttaa
    15961 gttggtagaa gaagaggtga agccgtgaaa attatcgttg acaatttttt tacgatcctt
    16021 ttgattatga atattttgct ttcctttatc atcgtttttc gagaaagaaa ggaaactgct
    16081 caaacatggg catgggtgct cgttttgatg ttcattccag ttgtaggatt tattttatat
    16141 atttttttag gaagagggat ttccaaagag aaaatctttg atttgaagat ccaagggaaa
    16201 atcggtaaga acctggaaat tgaagaagac aaacaggcat tgatgcgagg gctttatcct
    16261 catccgccta ctggacaagt agatgtgaaa caattgatct atatgctcac ggtatttgaa
    16321 agtacgctct atacgactaa aaatgaaatc actttattta cagatggtcg tgaaaaattc
    16381 gatgcgctga ttgaagatat caagcaggca gaagaccata tccacttcca gtattatatt
    16441 tatcgcagtg atgcattagg tgaagaagtt cgagatgcat tgacagaggc ggcaagacga
    16501 ggggtaaaag tcagagtcct tcttgatgca tggggttcca cacaagtttc ttccagcttt
    16561 tttcagaatt tgaaaaaagc aggcggagaa gtcgcatttt tctttcctct atttgttccg
    16621 tatattaatc ctcggatcaa ttatcgcaat catcgtaaaa tcgtagttat tgatgggaaa
    16681 atcggatata caggtgggtt caatgttgga aatgaatatc taggattggt aaagaaattt
    16741 ggttattggc gagacaatca tttgcgtatt tacggagaag cggtgtatac cttgcaaaac
    16801 cgttttttga tggactggaa ttctcagcat acaaaagaag taggctattc tcccaaattt
    16861 tttccgtcca ttcactctac tggtgatata gctgttcaaa tcgtgacaag cggaccagat
    16921 accgaacatg aacaaatcaa aatgacttat ttgaaaatga tttcgttagc caaacgggag
    16981 atattgatcc aaacgcctta ttacataccg gatggatcga ttcatgaagc actgaagttg
    17041 gctcttcttt caggcgtaaa agttcatatc caaataccta acaaaccaga tcatcttctc
    17101 gtttactggg caacgtattc tttcgctgca gagctaatcg agtatggagc aaggatcgaa
    17161 acatatgaga atggatttat tcatgcaaag acgatgatta tcgatggtgg cattgtttca
    17221 gttggctcag caaatatcga tgttcgttca tttcgcttgg actttgaggt caatacactg
    17281 gtttatgatg aacgaatggc ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt
    17341 tctacgcatc tcacaaaaga aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa
    17401 ggattagcaa gattgatttc gccgctcctt taaaacataa aaaggacatg agaaccatca
    17461 acagatggag atctcgtgtc cttttctaac ggggccattt ctgagagtac gtcagaaatg
    17521 gagaaagcgt ctcgcatgag cgaaattaac tttggggtat ctcttgtttt ttggggaaag
    17581 caagaaataa gctaatatat gctattaaaa gagacacatt tcaaagccag tatagcatag
    17641 aaacttttaa tgagatagtt ttttttacaa ttatgagaat ttattttgtt atttaatgat
    17701 tttgattcgt tttcatcaag taatgaaaat gataaaataa acatacttta ggattaaagg
    17761 agatttctaa tgaaaaaact taaattattc ttaccaataa taggattagc tattctttta
    17821 tcaggctgtt caaagtggat tgatcgagga gaatccatta cggctgttgg ctcttctgct
    17881 ttacagcctt tagtagaaac agttgctgaa acttaccaga cagaaaatcc tggaaaattc
    17941 atcaatgtac aagggggcgg aagcggaact ggtttgtctc aagtacagtc aggagcagtc
    18001 gacatcggaa actctgattt gtttgcggaa gaaaaatcgg ggatcgatgc ctcagcatta
    18061 gtcgatcatc gagtagcagt tgttgggatc actcctattg tgaacaagga tattggcgta
    18121 aaagacatca gtatggaaaa cctgaaaaaa ctatttacag gcgagatcaa aaactggagt
    18181 gaagtcggtg gaaaagatca gccagtagtt atattgaacc gcgcttcagg aagcggaacc
    18241 cgtagcacgt ttgaaaaatg ggtattggac ggagaaacag ctgttcaggc acaagaacaa
    18301 gattcaagcg gaatggtgcg ccagatcgtt gccgacacac ccggtgcaat cagctatgtt
    18361 gctttttctt atgtttccga tgatgtagat acgttgaatg ttaacggtgt agaaccgaca
    18421 gatgaaaatg tgacaacgaa caaatggacg atttgggcat atgagcatat gtacacaaga
    18481 gatcatccaa aaaaactgac aaaagaattt ttagactata tcttatctga tgaagtacaa
    18541 gataacatcg ttggagaact aggttatatc cctgtttctc atatgaaagt cgaacgtgat
    18601 tggcaaggaa atatcgttaa agagtaatct ttacacaaaa tttacaaact tagctaaact
    18661 tttacatgtt attaaaacca aattgatgtt caatttgtac actgagtgag tatgttaaac
    18721 ctgattaagg gggagtcatc tgtggaagat gtgaaaaaag tattattaac aaaatcaaaa
    18781 cgttcaaaaa tggaacagcg tggaaaattc atcagttttt tatgtatcgc gctgatcgtg
    18841 ttggttgtat tatctatttt ttattttgta gctagtaaag gattggccac cttttttact
    18901 gataagatca atgtatttga ttttctgttt ggtacagatt ggaatccaag ccaaatcgga
    18961 gcaaatggga aaccaatggt aggggcattg cctatgatca caggttcgtt tattgtgacc
    19021 tttttatctg ctatcgttgc cacgccattt gccattggtg cagcagtatt catgacggag
    19081 atctctccta aaaacggaac gaagatctta cagccggtca tcgagctgct tgtaggtatt
    19141 ccttctgtcg tttatggttt tatcggttta tcagttatcg tgccttttgt cagaagtatt
    19201 tttggcggga ctgggtttgg tatcttagct ggtacatttg tgttgttcgt tatgatcttg
    19261 ccgactgtca caacgatgac tgttgatgca ttaaaggcag tgccacggca ctatcgggaa
    19321 gcttcattag cgcttggtgc cacaagatgg caaacaatct acaaagtcgt gctacgagca
    19381 gccgtgcccg ggattttaac agcagtcgtt tttggtatgg ctcgtgcgtt tggtgaagcc
    19441 ttagctatcc aaatggtcat cggtaatgca gcattgatgc caacaggtct tactactcct
    19501 gcttctacat tgacgtccat cttaacaatg ggaatcggaa atacaatcat gggaacagtt
    19561 gaaaataatg tgttatggtc attagcgctg atcttgctat tgatgtcatt attctttaat
    19621 atcgtgatcc gtatgattgg gaagaaagga gagctgaaat aatgaacgca aaacgctcag
    19681 ataaaattgc tacaggtgtt ttatatatag tttcggggat catcgtattg attttagcag
    19741 ctttactact ttatatctta gttcggggaa taccgcacat ttcatggtcc ttcttgaccg
    19801 aaccttccaa agcctaccaa gaaggcggtg gaatcgggat ccagctattt aactcgattt
    19861 atttgctttt gatcacgatg atcatcagtt ttcctatttc tttaggtgcg ggtatctatc
    19921 tttcagaata tgctggaaaa aactggctaa cagatgtgat tcgcacgtcg attgaaatct
    19981 tgagctcgtt gccttctgtc gttgtcggtt tattcggttt cttggttttc gttattcaat
    20041 tccagtatgg tttctcgatt atttctggtg cattggcttt gactgtattc aacttgcctc
    20101 ttttgactag aacagttgag gagtctttac aagcggtcca ttacacacaa cgagaagcag
    20161 gattggcctt aggtttgtca cgctgggaaa cagtgataaa agtggtggtt ccagaagcga
    20221 caccagggat tttgactggt gtgatcctaa gttctggaag aatttttggt gaagcagctg
    20281 cattgatcta tacggctgga caaagtgcac ctgcgttaga ttttacgaac tggaatccat
    20341 taagtgtttc aagcccgatc agcattttcc gtcaagctga aacattagct gtccatattt
    20401 ggaagatcaa tacagaaggc acgatgcctg atggtgtttc tgtttcggct ggcgcttctg
    20461 ccgtgttgat tattgctgtt ttactgttca acttcggcgc tcgtgcgatt ggcaaacgat
    20521 tataccgaaa aatgacttca gcctaaggag aagaagcaaa atgaaaaaat ataactggaa
    20581 tgaaacaaat gtcatcacca tgccaaaaga gaaagaagta gcactatata cggatgatct
    20641 acatgtctgg tatggcgaca atgaagcaat caaaggagtg gatctggaat ttgaaaaaaa
    20701 taagatcact gcactgattg gtccttccgg ctgcggaaaa tctacgtatc ttcgttcgtt
    20761 gaatcgaatg aatgatggga tcgctaatac gaaaatcact gggaaaatca tgtatcaagg
    20821 cgtagatatt aatgcgcctc aagtggatgt ttatgagatg cgcaaacgga ttggcatggt
    20881 ttttcaacgt cctaatccgt tcagcaagtc gatttatgac aatattacat ttgctttgaa
    20941 acagcacggc gaaaaaaaca aaaagaaatt agatgaaatc gttgaaacaa gcttgaaaca
    21001 agctgccttg tgggaccaag taaaagacag cttggacaaa agtgccttgg ctttatctgg
    21061 cgggcaacag caacgtttat gtattgctcg tgcgatcgcg atgaaacctg atatcttgct
    21121 gctagacgag ccggcaagtg ctttggaccc tatttcgaca ggaacggttg aagaaacact
    21181 tgtaaacttg aaagaagact atacgatcat catcgtgacg cataacatgc aacaagcagc
    21241 tcgtatcagc gactatactg cctttttcta tttaggaaaa gtaatggaat atgacaaaac
    21301 taacaagata tttaccagac caaaaattca ggcaaccgaa gattatgtgt ccggtcattt
    21361 tggttaagac ggaggcctca acatgacaaa agagattatt tcatctaaag atcttcatct
    21421 gtattatgga aagaaagaag cactaaaagg gatcgatcta agcttcaatc aaggggaaat
    21481 cactgcgatg atcggtcctt ctggttgcgg aaaatccact tatctgcgtt ctttgaaccg
    21541 gatgaatgac ctgattccga atgtaacgat caccggaagt gttttatata aagacaaaga
    21601 catctatggt ccaaaaacag atgttgttga actaagaaaa gaaatcggca tggtattcca
    21661 gcagccaaac ccatttcctt tttctatcta tgaaaatgtc atctatggat tgaaattaaa
    21721 aggggaaaaa gataaaaaag tccttgatca agtagttgaa gaaagcctga aggcagcgtc
    21781 tgtttgggat gatgtgaaag acaaattgca taaaagtgct ttatctttat ccggtgggca
    21841 gcagcagcgt gtatgtatcg cacgtgtttt ggcagttaat ccggaaatca tcttattgga
    21901 tgaaccaaca agtgcgcttg atcctgtttc tactgggaaa atcgaaagta tgctgttaga
    21961 attaagagag cggtatacga tgatcatcgt gacccataat atgtctcaag cttcacgtat
    22021 ttcagataag acggcattct ttttagatgg tcacttgatc gaatacaatg atacgaagaa
    22081 gatattcatg aatccagaaa aacaagagac agaagattat atttcaggtc gatttggata
    22141 aggaggaaac ggaatgctac gtacacaatt tgaagaagaa ttgctgaatc tccataatca
    22201 gttctacgaa atggggatga tggtgagcag cgccgtgcac aagtcagttc gtgcatacat
    22261 caaacacgat aaaatcttag cccaagaagt tatcgaaaac gatataaata tcaataacat
    22321 ggaaacacgc ttggagaaga aaagttttga aatgatcgca ttgcaacagc cagttacaac
    22381 tgatttgcgt atgatcatta cggtcatgaa agctagctcg gatttagaac gtatggccga
    22441 tcatgcggtg tctgttgcta aatccacgat tcgcgtaaaa gggcagacga gaataccgga
    22501 aatcgaaaaa gagatctccg atatgtccga ttatgtgaaa aaaatggtgg acaacgtcct
    22561 agttgcttat gtcaaaacag acgaaaaaga cgcacgtacg atcgccaata tggatcaaag
    22621 agtcaatgac tatttcaatc gaatctataa tgcaacaatc aaaaatatgc aggaaaatcc
    22681 tgagacagtc atcagtggaa cagactactt gaatgttgcc agttatctag aacggatcgg
    22741 agactatgtg acaaacatct gtgaatggat cgtctacttg gctacaggga aaatcactga
    22801 gttaaatacg aaccacaatg agattttcta aactgaagct gtgattatgc cgtttgttta
    22861 ggaaaagagg ttgtgaaatc agcggttgtg aaaaagctgt tctgcatcaa gtaataagaa
    22921 cagtcaaaca aaaaatagct cctcatattt ttcgtttggc tggacttatt accgcagatg
    22981 caagcttttg aacaccgttt attcgggaaa gggtttgtga caaaacatgt cacaaaccct
    23041 tttcatcatt tcatagaaag gataaagaat gcaaaaaaaa attagcatca tcactatatt
    23101 ttttatttgt gtgcttactt catgggttat tcagcatcaa gccgctttat atgaagtacc
    23161 tgtcatcgag atagaaaaag tacgaataaa agaggatcat caattgatta caggaaaact
    23221 gatgaacacg gatcaaaaag gagaatcgat ttatttatcg gttccttatc aaaaaaatca
    23281 aatagaaaca gcaaatataa aagcgagaca aatgtatcta gtccaaatgc atggagatga
    23341 atggcagcta gtagcaaaaa aaaatgatgg ctggcttttc ttcttcacaa gtatcttcat
    23401 tggcgccatg ttgcttattg gtggaaaaca gggtctattt gctttaggaa gtctcatcat
    23461 caatatcgtt ttacttttac ttttgttgct catctttact aaaacagaag ggatttctct
    23521 attaaatata tctatcctgt tcgtcgttat agggatgatt attagcatat tggctttaga
    23581 tggatggaat agaagcagta taatcaaaat ttcagctgca gttacgagtg tttttttagc
    23641 attttttatt tgtcttttga caatgaatca ttggaaagac caaggactgc gctatgagga
    23701 actgaattat ctgacacgac cgtatcgttc tgtttttctt tctagcgtac tgattggaac
    23761 agctggagga acactcgata ccgttatcac agtcattgcg acattagaag aattgactaa
    23821 gcgacaacca gaaataacag cgaaacagtt atggctttca ggaaggcaag tgggtcgaga
    23881 tgtgattggc tcgatgacca atatcctttt gttcgtctat atcagtgggt cagttccatc
    23941 gcttctttta tatttaggaa atcaatggtc gttcaaagaa acgatagaac agcatttatc
    24001 attggaattt ttacgcgtca ttgctggcag cttaggaata gtattatcga tccctatctc
    24061 ggtcctcttt tttctgactg taaggaggct gaaacgatga atgtacttat tatacttgtt
    24121 gggattttag caatcagcgt gctacttatt ggcggtacgc aaggtatgta tattctttta
    24181 ggcttattca tcaatttagg gatctttttc ctgttgttat tcggttatca tcagaagtgg
    24241 ccgatactgg cattgtcggt cataggattt ttattgattg ctgtggttat cttatttttt
    24301 atcaacggtt ataatctaaa aatgcgtgca gcttttgtga gtatactgat ctttttattt
    24361 tgtttcctac tattgcttcc tatcactgat tttttagcaa tccaaggttt tacatcggta
    24421 gaactagaag aattatctgg cttagataag actttagcga ttgattttag attacttacg
    24481 agatcgttgc ttctgattag tcttagtggt gctgttttag atgcgagtgt agcaatttct
    24541 agtgggacat tcgaagtata tcaggcaaat ccgcaattat ctttttacca attgcgccat
    24601 tctagtttcg ctattgctaa aaaaatattg gcgtcaacag taatgacact tctttttgca
    24661 ttcatgggaa gttctttagc gttgatcctc tggtttattg atttagacat tccttttcag
    24721 caagtgatca atgaaaaatc atttgttcta gaatatacga tggctattct taccacattg
    24781 tccgcactac ttgttcttcc tttaacctgc gtgactacag catatctctt tacaaaaaag
    24841 aaagaacaac tcaagatgga gtaaaagtta caacttaagt cctatgacat ttttactgaa
    24901 tcagtgcata atatggatgt aaggaaaagc ttgcaaacaa ttttgcttaa ggaggcaatt
    24961 tccatgaaag aaagagaacg tattttagat ttagtgaaaa aaggaatcct ttcgactgaa
    25021 gaaggcttag atttattaga aagcatggca actgaaaaag acgaaaagca aatcaaaaaa
    25081 gaagcagaca aggtaactgc tagtcataaa gaaaaagatc aggcaagtca attgatcgat
    25141 aaacttgaaa acggcgaaga agaaatttct gagccagttg atccaaagga aagagaaaga
    25201 caagatcaag aaaacttaga aaagatctta gatgaactag caactgaagc gaataaaact
    25261 tcggcgcgtc tagatgaagt aaatgctcaa ttcgctgaca ataaggcagc acgcaatgag
    25321 aaacaggaag cattgatgca gctgaataca aaagaagaat taggcgagtt gacagaagaa
    25381 gaattagcac agcgtcagac gttagaagca gaaatcaaag aattagaagc tagtggcgat
    25441 acactgttag aagaacaatt gaaattagaa gcagaattaa aagatatccg taagaatcaa
    25501 tggtctgaga aaaaagaaac gtttactgat aagtttgaat tgccagatga ttggaaagat
    25561 caagcaacag atacactgaa tcaagtaggc gaaaaaatgt cagaagctgg atcacagtta
    25621 ggcaaattct tgaagaaaac attccaaact gtttcagaaa cagtgaatga caacatggaa
    25681 tggaaagatg tcagcttacg tgtccctggg attgcgacaa cgaaatttga gcatgaattt
    25741 tactatgaag ctcctgcagc aagtatcctt gatatcaagg cagcaaacgg aaacgtgacg
    25801 ttaaaaacgt gggacagcaa cgatgtgaaa gttgaagcta agatcaaact ttacggaaaa
    25861 atgggtgctg aaccatttga agcattctct gaacggagcc agattgaggt taatgaagat
    25921 catatctcct tccaaattcc aaataaacgt gtgcgtgcag accttgtttt ctacttacct
    25981 aaacgagtat acgaccacgc agctattaaa cttctaaatg gcaacatcat gattgaaaca
    26041 ttggaagcta aagatattta tacaaaatct acgaatggta acatcatcgt ggaccaatta
    26101 actgcaacga tgctggaagt agaaggtgta aacggcaata tcgatattcg aaacgggaac
    26161 atcttagatt ctattatcga aacagtaaat ggtacagtga catttggtgc gacagcagaa
    26221 aacctttctg tgtctttggt aaatggtgat gtacgtctta cgattaaaga agataatttg
    26281 aaaaaagtag aagcaagttc agtgaacgga aatgtcaaag tcgctttacc agatggcatc
    26341 ggtttggaag gacatgctaa aacaagtctt ggaagtatca acagccgtct gtccaattac
    26401 gaagtagttc gtgagaaaaa agaacggaca aaccaaatgt tgcaattccg tcgtgtttca
    26461 gatggtgaaa tcgcgcaagt gcaactttca acgacaacag gaagtatcta tttaaaagat
    26521 acagataaat aaagaggttg tgaaagagat gttcagcctt gagcaataag aagcgatttt
    26581 gtaaaatagc ttcccctatt ttacaaaatt gtgccttatt gccgatagtc tatctcttga
    26641 acaccgttta ccaagagatt atgaacctgc caattatcgc cgagtcacaa ggaataattt
    26701 cagaaaaagc tcgtcacgtt ttatgaatat ggtaatttgt gaccgagaat tttgcagaaa
    26761 gacaccgatt aagaagaagg ttgtcacaac gattttcttc atttaaaata gtagaacaga
    26821 ggtgagacat gatgaaaaaa aagttgacaa aatctaataa aaatgttgtt ttgacaggga
    26881 ctttagcagg aataggagaa tacatcggga ttgatccgac tgttatccgt gtgctttatg
    26941 tattcctgag cttagttgct tttggaagtc caattatttt atacatttta ttagctttga
    27001 ttatcccatc tgcgcgttct gcacgaacaa actatggaca taacaatgca tattatgaaa
    27061 aaaacaatta tcgtgaacaa actcgtgaac aaacaaaaaa acgcaaagaa gcggaaaaaa
    27121 tcgatgatga cgaatggagt gacttttaat gtcgtacttt caacgtattt tagttaatac
    27181 gttaacattc atatcattgg ctgttctttt tcctgcgatg gtccatgtga gcagtatttg
    27241 gacggccgtt cttgccagct tcgtattgtc tgtgttgaat atgtttgtca aaccgatttt
    27301 gacgatactg tccctgccct ttacattagt cacattggga ttgtttacgt ttgttatcaa
    27361 tgcagcgatt cttaaaatga cctccttttt tgtgggagca gaacatttta gtttttcttc
    27421 attcggtgca gcgttgttag tagctatctt gatgtcgctt gtcaatgtaa ttgtcagcga
    27481 acataacagt agaaaataaa agaaagctgt gccgaactat cggcgcagtt tttttattta
    27541 gaatcaggaa tgcgttttct attacattgg taattaaata aaaacgtttt ttttaattgg
    27601 tagtactttt ggctagacaa tgataaaatg atagagaaca ctctaataga aatgaggtag
    27661 catgaatgtc agaagttgta aaagttagcc aattagtgaa aaaattatct ttggaaatcg
    27721 tgacaggcga cgagaaaagc ttagaccgtg tcatcgtaac tggggacatc tcacgcccag
    27781 gactagagtt gactggttat tttaattatt attcccataa tcgtttacag ctatttggca
    27841 gtaaagaaat cagttttgcg gaacggatga tcccagaaga gcgtttgatc atcatgagaa
    27901 gattatgtag tgaagatatg cccgcattta ttatttcaag aggattacca gctccagaag
    27961 aaatgatcca ggcagcaaat gaaaatggga tagctgtact gcgttctccc atctcaactt
    28021 ctcgtttatt aggagaatta tccagttatt tagatggtcg ccttgcccct cgaacaagtg
    28081 tccacggtgt tttagtggat gtttacggat taggtgtact gattcaaggt gacagtggga
    28141 taggaaaaag tgaaacagcg ttggagttga tcaaacgcgg ccatcgattg atcgcagacg
    28201 atcgagtaga tgtttatcag caagatgaat taacggtgat cggagaaccg cctaaaatcc
    28261 tggaacatct gattgaaatc cgaggaatcg ggatcattga tgtaatgaat ctttttggtg
    28321 cgagtgcggt acgtggctct atgcaagtcc agttggctgt ttatctggaa gcatgggcaa
    28381 aagataagaa atacgaccgt ctgggctctg aagacacgac cgttgaaatc gcggaagtgg
    28441 gcattccaca aataaaaatc cctgtcaaaa caggacgaaa cgttgcaatc atcattgaag
    28501 tagccgcgat gaatttccgc gcacgtacaa tgggctttga cgcaacgaaa acatttgaag
    28561 aaagattaag tcgcttgatt gaagaaaact caggcaacga ctagcaagga gtgacaatat
    28621 gttagcacaa atcaatcgag ttgcttttga gcttgtaggg attcctatct actggtatgc
    28681 attgatcatc gtatcaggta tcattatcgc tatgtggctt agcagcagag aggctgttcg
    28741 ggtaggatta aaagcagatg atgtgacaga ttttatgttg tggggattgc ctatctccat
    28801 tattggcgct cgcttatatt atgttttatt tgatcttccc cagtatatag ctgatccaat
    28861 ccaaatattc aatatccgtt caggaggctt agctatttat ggcggactga ttgcaggcgc
    28921 gatcacgctg tattttttta ctaaatatca ttttatttct ttgtggactt ttttagatat
    28981 cgctgcacca agtgtattat tagcacaggc aatcggtcgt tggggaaatt tcatgaacca
    29041 cgaagcatat ggaccagaca cgacgcgaag cttcttagaa agtctgcatt taccgcaatt
    29101 catcattgat aatatgtata tcgaagggat ctatcgtcaa ccaacatttc tttatgaatc
    29161 actttggagt ttgattggat ttattttgct tttgttttta cgaaagaaac cacacctttt
    29221 gaaaaaaggc gaagtccttt tatgttatct aatgtggtat agtttcgggc gtttctttat
    29281 tgaaggatta cgtacagaca gtctttactt tttaggtgtg atccgtattt ctcaattatt
    29341 atctgctgtg ttatttgtcg gtacattgat tctatttatt tggcgcagaa aaaaaggaaa
    29401 tttaccggat tatgaccgct cattaggaaa aaatcaacaa attatctgat ttttagagcg
    29461 ttaaaaatga aaaaaatttc aggagtggta aaatgaatta tagagtagcg gttttaggac
    29521 caggctcttg gggtactgct ttggcacaag tcctcgccga aaacggacat gacgttcgga
    29581 tttggggaca tcgtgcttca caagtagaag aaatcaacac ccaacacacg aatcaacgtt
    29641 atttaccaga tcattatcta cccgattcca tccaagcatt caccaatatg gaagatgcag
    29701 taaaagacgc agatgcggtg ctattcgtca ttccaacgaa agcaatacgt tctgttgcaa
    29761 aagaactagt gcctaaattg aatacaaagc cagtgatcat ccatgcaagt aaaggattag
    29821 aacagggaac acataaaagg atctctgaca tcttgttaga ggaaatccca gcagaaaaaa
    29881 gaaaagctgt tgttgttctt tctggaccaa gccatgcaga agaagtagca aaacatgaca
    29941 tcactacgat cacagctgca agtacagatg caaaagcagc aaaatatgta caagagctgt
    30001 tcatgaataa ttattttcgg atctatacca atcctgatgt gatcggtgta gagatggggg
    30061 cagctttgaa aaatattatt gctattggtg ctggcgctat tcacggctta gggtttggcg
    30121 ataatgcaaa ggcagcaatc atgacgcgcg gattagctga gatcagccgt ttaggtgtag
    30181 caatgggtgc caacccgctg acctttatcg gattaagcgg aataggcgat ctagtcgtta
    30241 cttgcacaag tgttcattct aggaactggc gcgcaggcaa cttgttagga caagggcagc
    30301 ccctagaaga ggtgctagcc aatatgggaa tggtcgttga aggtgtgaat actacccaag
    30361 cagcgatgga actagcccaa atgatggaaa tcgaaatgcc catcactcaa acaatctatg
    30421 aagtgctgta tgaacaaaaa gaaatcaaaa cagccgcaaa agaaatcatg ttacgtgatg
    30481 gaaaaaaaga aaatgaattc tcattaacaa ttttttgaag gagcgaatgt atatgaaagt
    30541 taaaaaagca gttattccag cagcaggatt aggaacaaga tttttaccag caacaaaagc
    30601 tatggcaaag gaaatgttgc caattgttga taaaccaaca atccagttca tcgttgaaga
    30661 agcgttgaaa tcaggtatcg aagacatctt gatcgttaca ggaaaagcta aacgaccaat
    30721 cgaagatcac tttgattcaa atattgaatt agaaacaaac ctaagtgaaa aaggaaagac
    30781 cgaattattg aaattagtag aagaaacgac agatgtcaac ttgcatttca tccgtcaatc
    30841 tcatccaaag ggtttaggac atgccgttct tcaagcaaga gcctttattg gaaatgaacc
    30901 atttgtcgtt atgctaggcg atgatataat ggaagacgaa gtaccattga cacagcaatt
    30961 aatcgatgat tacgaacgta ctcatgcttc tacgatcgcc gttatgcaag ttcctcatga
    31021 tgaaacttca aaatacggaa ttatcaatcc aggagaagta ttggaagatg ggttatacaa
    31081 tgtcaaaaac tttgttgaaa aaccagatcc aagcaaagca ccaagtgatt tagctattat
    31141 tggacgttac ttattgactc cagaaatttt tgatgtattg tccgagcaac aaccaggagc
    31201 cggaaatgaa atccaactaa ctgacgcaat cgatacattg aataaaacac aacgcgtatt
    31261 tgcccgtaaa ttcacaggta aacgttacga tgtcggtgac aaatttggtt ttatgaaaac
    31321 aagtattgaa tatggtctaa cacatccaga agtaggtgca cctttacgtg agtacatcct
    31381 gtctttaggt aaaacattag aagcagaagg aagcaagatc gatcataatc caaaaggaat
    31441 aacagaaatc aaagaagaaa agaaaaaata accctttttt ctaacgagct tcagctgcta
    31501 attgaataaa aagtagttga agctcgtttt tatttgttta aaaaacacga aaatgctatc
    31561 cttatagaaa agagagacaa gaagggagtt tgatcatgtc aggaggagaa attgcaggat
    31621 tgatcgcagc aatcgccttt gcgatacttg tcgtttttat cgttcgtgtg ttgttgcaag
    31681 tttctaaaac tttagaaaaa gtagatcaga ctgtagcaga agcaaatacg acgatcgaaa
    31741 tcgtcacaaa agacgtagat cttttgtcta gacaagtaga aggcttactg gtcaaaagta
    31801 atgaactgct cttggatatc aatggaaaag tcgcaacgat cgatccattg ttcacagcag
    31861 ttgctgattt aagtgaaagt gtatcagaac ttaatcattc tagtaaaaac atcgcgacga
    31921 aagtcggcgg aatcggtaaa acgacagcta aagcgacggt tgccggaaaa gtcggtggga
    31981 cagcgatgaa attttttaaa aacaaaaaac atactgaaac gacaggagga aaataaaaat
    32041 ggctaaaaaa ggtggatttt tattaggtgc agtagtcgga ggcacagccg cagcaatcgc
    32101 cgcgttattg ttagcaccaa aatctggtaa agaacttcgg gatgaattag ctgcccaagc
    32161 ggatgatttg atggatatgg cttcagatta cacagatatg gcgaaagaaa aatcgactga
    32221 gttaacaggt attgcaaaag aaaaagcgca agatctttct caacaagcag gtgatctagc
    32281 aggtagtgtc aaacaaaaag taagtgaatc agcagatgaa gcaggaagtg aatctgacga
    32341 tatcttgtta aatcttaaag aacaatcagc tgacttatct aatcgtttca aaaaaacggc
    32401 agaagatatg aaaaaacctg cagatgaatt aggaagtatt gctaaagata cttcagaaga
    32461 tatcatgatt gatgtaaaag acgctgctgg taaagcagga gatactgtca atgaaggaat
    32521 gtcagaagca aaaccagttg cagatgactc aaaagaaatg atgaaaatgg caaaagaaga
    32581 tgcgaaagaa gctaaaaaag aaataaaaga tacaatgaca gattcatcta caaatcacta
    32641 aaatcacaaa tttatagaat aaaaaggtgg gagcggacta gctctcacct tttttactct
    32701 tatttttcta aaacaagtaa ttcttttggt gtagtcgtga atggcacaca gccatcgctt
    32761 gtcacatata cacagtcttc gatacgtacc cctacttttt caggaatata gattcctggt
    32821 tcaagagaga agcacatccc ttcttcgatt actaagtcat ttccttctac aagggaaggg
    32881 aattcgtgga cggttgttcc gatcccgtga cctaaacggt gattgaaata ttcgccgtag
    32941 ccatagctgt tgatcacgtt tcgtgcgatt tgatcaagtt cacctgctgt cacacctgga
    33001 cggacggctt cttgtgcggc aagctgtgct tcaagagtga tgttgtagat tttttcttgg
    33061 aagtcggttg gtttttgata agcaattgta cgagtagcat cactgcaata gccattccaa
    33121 acaacgccaa gatcgaataa cacaagttca ttcggtgaaa tcttcgtatt tcctggtgta
    33181 ccatgagggc ttgcagcatt tgctcctgca aggactaatg tgtcgaaaga catttgtgaa
    33241 acaccttgtt ttttcaattg gtattcaatt tctgcaatga tcgcttgttc agtcacgcct
    33301 tctttcactg ctttaaagcc aatctcaaat gcaacatcgg cccatttacc agcttcgatc
    33361 agtttgtctt tttcctctag agttttgatc agttgtagac gttggatgat ggaagttaga
    33421 tcagtgtaag aatccgttcc cgtcaagata tcagagatag catcgaagcg ctcgacagtc
    33481 aaggcgttct tttcaatccc tacattgcca ggctgaccat agcgagcacg aagttctttt
    33541 ccgatgatct cccaagggtt ttcgctgtct aaatagccac ggacatcata cgcccaacta
    33601 ctgttttgag catcttcaac ttccaaagca ggagtgaaaa ggaaaggatc ttgttcaaga
    33661 gggataaaaa gagctagtac acgctcatga ggatcacttt cgtatccgga aaagtaagcg
    33721 atgtggccag ggtcgctgat ataagccaat tcgacttggt gttgtgccat ccattgacgt
    33781 agttcattga tttttacgtt attcatttgg caacctactt tctaaattaa ttttgtattc
    33841 aataattgta ccactgattt attaaaagac caacgttcta aaaactgaaa aaagtaaaaa
    33901 ttttcatgaa aacagagata aaacggttgc aattcttgag attatttgat attctatttt
    33961 agaaatgtaa gacgtatttt cagaaaacat ataaaatatg aataggagaa cacaaatgga
    34021 aaagcaaaca atcactatct atgatgtagc gagagaggca aatgtttcga tggctactgt
    34081 ttcacgtgtc gtaaatggca atccaaacgt taaaccagca acaagaaaaa aagtcttgga
    34141 agttatcgat cgcttggatt atcgtccaaa cgcagttgcg cgtggcttgg caagtaagaa
    34201 aacaacaaca gttggtgtaa tcatccctga tgtcagcaat atgttctttg cttctttagc
    34261 acgtggtatc gatgatgtgg caacaatgta caaatacaat attatcttag caaattcaga
    34321 tggcaacgac caaaaagaag tcaatgtatt gaataactta ctggctaagc aagtggacgg
    34381 cgtgatcttc atgggtcacc atatcacaga cgaaatccgt ggagaatttt cacgttcaaa
    34441 aactcctgtt gtattagctg gctctattga tccagatgaa caagtaggta gcgtaaacat
    34501 tgattatgta tccgcaacaa aagatgcagt aaacaaattg gcaaaaagcg gcaataaaaa
    34561 aatcgctttt gtcagtggtg cgttgatcga tcccatcaat ggacaaaatc gtctgaaagg
    34621 ctataaagaa gcattaagcg aaaataacct accatatagc gaaggattga tctttgaagc
    34681 acaatacaac ttcaaagatg gtctatctct agtcgatcgg atccataata gcggtgcaac
    34741 agctgtctat gtttcagatg atgaattagc catcggtatt ttggatggtt tattagaccg
    34801 tggcgtgaaa gtaccggaag aattcgaaat catcacaagt aacaattcac tactgacaga
    34861 agtagctcgt cctcgtttaa caagtatcac acaaccatta tacgatttag gggcggtatc
    34921 tatgcgccta ttgacgaaaa tgatgaataa agaagaagtc gaagaaaaaa cgattatctt
    34981 gccttatgga atcgaagaaa aaggatctac aaaataataa aaggacaacc attcagaggt
    35041 tgtgtgcctg ccgtttaact ctgcgttaca aggagcaatt tcaaaaaaca gcatctcatg
    35101 tttgatgaat attgtgactc gtgattcgga gttggcagga gaacaccgtt tatttgggaa
    35161 aaggggttat gacaaaattt tgttataatc ctttttttgt gtcttaaaaa agagagaaag
    35221 tggttgaaat tacatttatc gtatataaaa caatccgtgt atagatgctg cattttgttc
    35281 accaccatca aaaaaataag ggactgtgac aatactattg tcacaatccc ttattactgt
    35341 gtattctatt aaatcaatca tctttctcat ctgattcttt agttgcttga ttcgctcttg
    35401 cataagctga agcggtgttc cagtaatctg tatagttttc atcggtaccg ccaacgccaa
    35461 atttaaaggc gcttttcttc ggtccatttt tcgcccatag cgattcaact gtttttcctg
    35521 gtgtttggat ggatttttta ttgacagtaa cttttccagg tgtttgacca gtaaatccag
    35581 atactttttt cttcatcaca tcagatgata attcgaattt ctcatcagtg ccaaaaatgt
    35641 caggttttgc ttggtaagct tgattgatca gattggcaag ataattagcc gaacgtatcc
    35701 ttgctttgct gtccattcca gtattgtcat cgtgccctgc ccagctactg atggtaatag
    35761 acggagtaga gacaattagc catgaatctc ggtattcatt ggtagaacca gtttttccga
    35821 cccaatctgc ttttcctaga tttccgttta agcttgagat ggcatctttg aatggtgtag
    35881 tgatttttgc attgatcaca ctacgcatca tgtcattcat gatcgatgcc gttgcttccg
    35941 aatagatccg gacagagttg ctttcgtgtt tataaattac atttcctgcg ttatctgtga
    36001 tggaatcgat gatatagcct tcttcataga cgccgccgtt tgctaaagcc tgaaatccgt
    36061 tggtctgttg aagcgtcgtg acatctactg tacctaaagg agcagattca acgccccaat
    36121 tgtcatttgc cgggtagttc atctttgcca agtagtgctc gtaagcatat tgtttcgatc
    36181 caccggaatt caacacatct tgatataagt gataagccgg gatattgttg gaccattcta
    36241 gtgactcacg gacggtttgg aaggtgttcg atccttcgtt agtagcattg acgattttct
    36301 cgccagcatt ttctccttct tgccaagttg ttgcataatc tgatacacgg gattctgatc
    36361 cgataagccc catgtcgatt gcaggtccat aaacaagaac tggtttgatt gatgagccag
    36421 cttgacgttc tgtatcgaag gcatggttat tttgattctg actgtaattc cgtccgccaa
    36481 cgaatccgta gatgcggcca gttctgttat ccatcaatac atttccggtt tctacttgac
    36541 tgccagcacc atcatctaac atgtatccat aattttcaac gccattttgc aatgcagcat
    36601 agacatcttt atcgattgtc gaatggattg tatatccttt attttggatc gtttgctgtg
    36661 ccttttgata ataagcgtca ctgaccgatg aatcagacat atcagcgttg tctttttcag
    36721 ccaattgttg tgcaatgatt cttgttgctt cattcatcac agtataatac aagaattctc
    36781 tgtcagtctg ttctgcgatt tgttgtggta gaaagtcttt tgtaaggtca taggcttttg
    36841 cttcttcgta ttgttctttc gtaatttggc cttcacgata catactgaat aaaacaaaat
    36901 cttttctggc aaggccggca gaaagatctt cttttagcgc gcctgtgttc gtgtatggac
    36961 tatacgtgat cgggctttgg ggtaatccag cgatataagc agcttgcggc agcgtcacat
    37021 cttttgcatc tacaccaaag attccttttg cagcttcctg gactccagca atgttttgtc
    37081 ccttattatt acgaccaaat ggtgagacat tcaaatacgt agcaatgatc tcatcttttg
    37141 aaaagtattt ttctacttgt gcagaaagaa tgatctcgtt tgctttacgt ttgaatgttg
    37201 tttcatctgt aaggatctgc tgtttgacta attgctgtgt caaagttgat ccaccagaag
    37261 agccaatacc agtagcttca gatactaatg ctcgtaaaac agctttagga acgtaacctt
    37321 ggtgtttatc gaagtattca tcctctgtgc tgataattgc agttttcaat aaaggtgaaa
    37381 tcttatcaga tgaaatagtc gtgcgcatca gatctgtctg gatttttgat atctctgtat
    37441 tatctgcata aacaatcttt gacgtttctg taatgtttcc tagttctgtc tgcagtgctt
    37501 ttttagtcgg caattgtgtg tcttcgacga gataggcaaa atagccagcg cctattccaa
    37561 tacctagagc tgcgaacatc aaaagaagaa cagtaataaa aacagtcaat gattgaaaaa
    37621 ctctaaaaat aatcttagga acaaaccatt tttctttttt tcttctattc tgtttttttc
    37681 gagtattttt gcgtggcaaa agggtcacct caactaatgg ttttatttcg ctaattatag
    37741 caaataatag aacaaaaaaa cagacaaggc tgtttttggc aggaatttct tttttaaaag
    37801 agttaaaacg tgagaaaatg atctttatta taaaagagtt cttaagcttt tctttagaga
    37861 aaatttcagt tactatggag aacagggcaa agaaaaatca atcattttct ttgtatatca
    37921 tcttaccaat atataggaat tgttagtaac aattgccttt caaggaggaa tccatccatg
    37981 ttattaggct cacacgtcag catgagcgga aagaaaatgc tgttaggagc agcagaagaa
    38041 gcagccagtt atggatcttc gacttttatg atctatacag gcgcccctca aaacactaga
    38101 agaaaagcca tcgaagagat gaatattgat gccggaaaag cgtacatggc agaaaaaggt
    38161 ctaacaaata tcgtcgttca tgccccttat atcattaatt tggggaatac gatcaaacca
    38221 gaaatgtttc cttttgccgt ccaatttctg agagaagaga ttgttcgtgc ggaagcttta
    38281 ggcgctactc aaattacact tcacccaggt gctcatgtag gggcaggaac agaagccgga
    38341 attgctagaa ttattgaagg cttgaatgaa gttttgacaa aagaacagac ggcacaaatc
    38401 gctttggaga caatggcagg taaaggtacg gaaatcggtc gaacatttga ggaattagcg
    38461 caaatcatcg atggagtaaa attgaatgaa aaattatccg ttacttttga cacctgtcat
    38521 acgaatgatg ccggttataa tattaaagaa gacttttcag gtgtaatgga agagtttgat
    38581 aagatcatcg ggttagatcg gcttaaagta ctccatatca atgattctaa aaatccgcaa
    38641 ggttcgcata aagaccggca cgccaatatt ggttttggaa caatcggatt cgacacgctg
    38701 aataagatcg tccatgatcc aatgtttgac aatatttcga aaatcttgga aactccctat
    38761 gtaggcaaag ataaaaagaa agcttacgca ccatatggaa aagaaatcgc catgttcaaa
    38821 gcgcaaacat tcgatccaga agtgtttcct gagttattag cagaagaagt gatactctaa
    38881 gattttctat ctgacagcag caaaaaagga ccaggcacat tgccttgtcc ttttttgttt
    38941 aagaaacaaa ggagtacttt ttgcagtctt tttcatcatg catcttctcc cccctctttt
    39001 taaatttaga caaacaaggt atactggtag aaaagtaaaa aaacgaaaag tttcacaagc
    39061 aagtggcaaa acgtgattgg aaggacgaaa agatgctgaa cactttaaaa cccttgatca
    39121 accaactaaa aaagaaacat acatacttta ttcttggcat agccctatta tttacaatta
    39181 ttcgtttgta tttatatagt aaagcaatct atggaacaga catgtcggca acctatgatg
    39241 accagctgct gattcgctat gctcaaaata ttttggacgg taaatggcta ggggagtata
    39301 cgactacaac actttccaaa ggaatctcct acagcttatt tttagtactt gcacataaac
    39361 tgcatctttc ttatgcagtt ttgttcggca tgttcagtat ttttgccagt atattggttg
    39421 cactcgcgtt taaaccgatt gtgaagaaca agtatcttct tttaagtatc tatttttatt
    39481 ttttgttttc tccgattagt tttacacatg aatattccac tcgaacatat cgtaatgccc
    39541 tcgtgattcc ggctgttgag atcattatcg cttgtttatt agctatttat taccgcaaat
    39601 acggttctag cagaaaattg ctgccatggt ttttagggct ttctgtcgct tttcctttct
    39661 tttggttcat tcgcgaagat tctatctggc ttcttccttt tactgtagta gctttgggta
    39721 tcagtttcgt ccagatcttg ctagggcaag acttttcttt caaagaagta aagaagttaa
    39781 aaaaacgact ctttcttctc tcgaaacggg aatggctgaa actttcattg tttttacttc
    39841 ctatagttgg cttttccatc gtctatcaat ggatcgaata taaaaatgag acaacatacg
    39901 gtattgcaac catcaatgac cgttccagtg gagaatttgg acgtttgatg aaacaactga
    39961 tccgaataga tgatgaaacg aacttgaata aagaaaacag tgagatatgg gtctcacatg
    40021 ctgcactaaa taaagcatta gccgcatctc ctacattcca tactgtttct acccgtattg
    40081 acgaattata tacgactcac gcgtggactc aaggtggaaa agtaaaagaa ttacccggag
    40141 atatcatctt ttgggcattg cgagatgtgg taagcgaatc tggttattac caaaacaatg
    40201 caaaagaaac caatgatttt tggaaaaaag tcaaccaaga attagaagaa gcctatcaca
    40261 agggaacaat caaaaagaaa aaagagttct atctgatggg atcaggagat ggaaagacga
    40321 tgagtgatct gccaatcgta ttcgatttcc ttcaatcagg atacgcttat gcatggcgat
    40381 ataaagattt ccatcaagga ggaaattata gtttcggaac gcaagaacaa gtcaaacagg
    40441 ctcaagaatt attaaacctt ccgctaatgg agaattggat cgatagtaat gaaccgagaa
    40501 cacctaattt ccaattaaca aaatcagcaa aagtcgcgaa tgtcatcata aaaatttacc
    40561 aagttctggc tattcccgtg ctgattgctt caatagcagg gatattactt atgatactag
    40621 gaaccgtatt tgcaaaagct gatcgtgcct attatagttc ctctttgatt gtggtcaccg
    40681 gattaatact gacacaattg gtttttcttt tcggtgtctc atggttttgt tcttatgcgc
    40741 cagaaaaaaa ggactatttt ttaagtgtct acactggtgc aggcgtgcca ttaacgcaat
    40801 tcatcgtagt aacaagcatg ctagttttca gtaaattaaa tctgaaaaaa gctttatctt
    40861 ctaaataaaa agacccttgg tagctagatt tttaacctaa ctatcaaggg tctttgtgtg
    40921 gaaaaatcag tttttattcg atttcttcta ccgtttcctg caaaagctgt tcatattttt
    40981 tgtttccttt atataaatca ccaatcgtcc atgccaacag gagaaggaca agaacaatga
    41041 ttacataagc gatgtgatca gccaatgcta gttgagcgga agtggcttgc tcgccgaaga
    41101 aactttcgat ctgtcctggt aatcctcgta agttcaagta agttagacta atcacagaga
    41161 accagccaag aagttttacg atgaatgaat ttttaaatcg gctacccatc tccgcttggc
    41221 tgttggtcat catcagtaaa ggaatcatcg aaaacggcaa cgcaaaagcc aagaacacct
    41281 gggattcatt cattaagtta ttcaaagcgg catgttcttc gatcgttcct tttgtacttg
    41341 taaacaaaac acaaatcaat acaggaatca cagaaagcaa tcgtgtgacc agtcttctta
    41401 gccaaatcgg gatacgcata tggatgaatc cttccatgat gacttgaccg gtcaacgtac
    41461 cagtaatagt agagttctgg ccagaagcaa gaagtgctac agcaaataaa gtggacaaga
    41521 taccagattt tgctacagca atcaaaatac cattgctcat agctgatgga tcagataaag
    41581 cttcatacag accaaaaaag gaaggatctt ggacagaacc ggttttgaag acagcgacac
    41641 ccatgatcaa tagcagtgca ttgactagaa aagccattga caactggata ttagaatccc
    41701 aagtagaaaa acgtacagct tgtgctacat cttcttcgtt ttggtgatcg atcttccgag
    41761 tttgtgaaac tgctgaatga agatataagt tatgcggcat gactgttgca ccaataatcc
    41821 ctaaagctcc agtcagcgga gtttcgccgc cgatgaccgg tgagtctgag aatgtatgac
    41881 tgttagggat aaagccagca aataaactgt gccaatctgg gttagacaag ccaacttgat
    41941 agacaaaaac gaataaaatg acgaaaatta agcagacaac cagtgcttcg atcttacgaa
    42001 acccgacttt tgtcagtagt aagagaacga aaacatctaa tacagtaata aagacggcaa
    42061 tcaccaatgg aatatcaaaa agtaagtaga gggcgattgc tgcaccaatg acttctgcaa
    42121 tatctgttgc cataatggct aattcagtca agatccataa aatgacacct aatgtcttac
    42181 ttgtgcgtgc tcggattgcc tgtgccaaat ccatttggct cacgattcct aattttgctg
    42241 ccatatactg taaaagcata gcaatcagac tagacatcaa aataactgac atcaataaat
    42301 attggaaatt ttgtcctccg gtgatcgaag tagaccagtt acccgggtcc atatacccaa
    42361 ctgctactaa cgcaccaggt ccagagtaag caagaagtgt tttaaaaaaa cttttcccct
    42421 ttggtacatc aactgttcca tttacttcag ctaaagataa accatttgta tgttcaataa
    42481 gtcgatgctt atgaaattgt ttattggatt gattattcaa gaaaaaacca cctttcctat
    42541 ttatctacga ttacattcta cacctcaaaa agaaaaaatt aaagaaaaag attcggtacg
    42601 cctaattttt tttcatggcg cgtcttgcta gatgacgaaa gaaattttgt tctcctctaa
    42661 aaaaaccact attttttgca tgaaactaaa gaatacgcct ttcttttttc aataaacttt
    42721 agtaaaataa gtcattgaga gcaattcgga aatagaaagg agagatttat atgttatcaa
    42781 caggtattgt cgtacttatt gctattattg ctttgctaat cggcgcagta ggtggctttt
    42841 tcctagcgcg taaatatatg aaagactatt tagaaaaaaa tcctcccgtt aatgaggaca
    42901 tgttgcgatc aatgatgatg tcaatggggc aaaaaccatc tgagaagaaa attcgccaga
    42961 tgatgcagca aatgaagaac caagggaaaa aataggttct gacccaacga ctttttacgg
    43021 gctgattttt tcagtccgtt ttttatttcg taatttgtta gactattcag attattcagc
    43081 aaaaaagaca agcacagaca atcctaggct cttgataaag acaaaggagg agtaaaatgt
    43141 cgatttttag aaaattaggc tggttttttc gtcaggaaaa gaaacattat ttaattgggg
    43201 ttttctcgct attcgcagtg gccttggtac agttagttcc tccaaaagtg attgggatca
    43261 tcattgatga gatcgccgat aaaaatttat caataaagat cattttcggg tggatcttcc
    43321 tattgattat cgctgctttt ttacaatatc tttttcgtta tatctggcgg acgaatattt
    43381 ggggaagtgc agctcgattg gagaaagaac ttcggcagca gttattcaaa cattttacag
    43441 aaatggacaa tctcttttac caaaaatatc ggactgggga tttgatggct cacgcaacga
    43501 atgatttgaa tgcgatccag aatgtagcag gagctggtat cttgaccttt gcagattcag
    43561 tgattacggg cggaacaacg atcattgcga tgattttgtt tattgattgg cgcttaacac
    43621 tgattgcgct gattcctctg cctttgctgg ccgtcacttc acgagtgctg gggtcaaaac
    43681 tccatgatgc ttttcgtgat tcgcaagaag ctttttctag tatcaatgat aagacacaag
    43741 aaagtatcac tgggatcaaa gtcattaaaa catttggaca agaaaaagaa gatctagcgg
    43801 attttgaaga aaagattgaa gatgcaatta ttaaaaacaa acgagtcaat tttttagact
    43861 ccttatttga tccattcatt accttgatta tcggtctgtc ttatgttgca acaattattt
    43921 taggtggaaa gtatgtcttg gaaggcacga tctcgatcgg gcagatggtc tcatttatta
    43981 gttatatcgg tatgcttgtt tggccgatgt ttgcgattgg tcgattgttc aacgtattgg
    44041 aaagaggaaa tgcttcttat gatcgcgtca atgaactttt gcatgaaaag acgcatatcg
    44101 tggaacaact gggtgcagtg cagaccccgg caaaaggcga gctgaatgta gctatttcct
    44161 cttttaatta tccgaaagaa gaatcaccag cattagaaca aatcaagttt gcgatccaag
    44221 aaggagaaac attaggtatt gttgggaaaa ctggtgcggg aaaaacgacg atcatcaaat
    44281 tactgatgcg tgaatacgat gattatcaag ggaaaatcca atttggcgga cgttcgatca
    44341 aagaatattc attgaatgct ttattaggat caatcggcta tgtgcctcag gatcacttct
    44401 tgttttcgat gaccattcga gataatatcc gtttttctaa tccaaaactt tcagaagaag
    44461 aagtagagca tgcggcttct ttagcattta tcaacaatga aatcaaggaa ttaccaaaag
    44521 gttatgatac cttagttgga gaacgtggag tctctttatc tggcggacag aaacaaagga
    44581 tttcgattgc tcgagcattg atcaaaaatc ctgaattatt aatattagat gatgcactct
    44641 ctgcagtaga tgccaaaaca gaagaagcta tcttgcataa tttaaaagaa ataagaaaag
    44701 agaaaacaac tatcattgca gctcaccgtt taagtagcgt gatgcatgca aaagaaatca
    44761 ttgttgtcga tcaagggaaa atcattgaac gaggaaccca tcatgagctg cttgcactag
    44821 gcggctggta tgaacaaatg tgggagaaac agcaattaga agcgaagata gaaggaggcg
    44881 aggcctgatg gatgaaaatt atcaatcgga atggacaaaa tcgattccac ttaaagaaca
    44941 gaaaaagatc atcaaacgac tgatgaagtt cgccaaacct tttagaagaa cgttcatcgt
    45001 tgctatctta tttgcgtttg ctttgtctgt gatcaatgtc ctattgccaa ggatcatcca
    45061 aacttttatg gatgatcatc tggctaaaca gtcggctacg acacaagtta ttttgttttt
    45121 tgctggtatg tacttatttg gagtcatagt aaaaagtatc atttggtttt tccaatggta
    45181 tttgtattcc atggcctcat tgaagaccta tcagtatatt agggtaaaat tatttgaaaa
    45241 gctgcataca ctaggtatgc gttattttga tcagacaccg gcaggatcaa ccgtttctcg
    45301 agtgacgaat gacacagaaa cgctatttga attttggtac gtctttttga tggtattgac
    45361 tgggatcttt gcagtcgttt cttcattcct tgccatgttt caaatcaatg gagaaattgc
    45421 cttgtggact ttggtgtttt tgccgatttt aggaatcgtc atctggtatt accagaaatt
    45481 cagttctcgt atttaccgaa atatgcggga aaaattaagt cagctgaaca cgaaattgaa
    45541 tgaatcgatt tccggcatgc gtattatcca gcagtttcgc caagaagctc gtttagctaa
    45601 agaatttgaa caagtgaaca atgagtattt agacacgcgt tatgcgatga tcaaaacgaa
    45661 ttcgattctg ttaagtccga tcatcaattt tctatatgca ttggcgattg cgttggtgtt
    45721 gactttgttt gggatcgatg cgttgcattc tcccgtagaa gttggactga tctatgcatt
    45781 tacgacctat gtccagggat tttttaatcc aatgtctcaa atgatggact ttttgagcat
    45841 cttcacagat gggatggttg ctggtagtcg tatcttgaag attatggata cggaagaata
    45901 tgcaccacaa caaaaccctg gagcagatca aatgattact tcaggtaaga ttgagtttcg
    45961 caatgtaagc ttttcttatg atggagagca caatgtatta aacaatatct cttttgttgc
    46021 taatccggga gagaccgttg cattagtggg acacaccgga agcggaaaaa gctcgatcat
    46081 caacgtattg atgcggtttt acgaatttta tgaaggtcag atattgattg atgatcatga
    46141 cattcgagaa tatccaatgg aagagttacg taagaaaatg gggctggtct tgcaagatgc
    46201 tttcttgttt tatggggata tcgcaggaaa tattcggatg atgaatccag ctatcacaga
    46261 cgaacagata cgccatgcgg ctgagtttgt tcaagcaaac aaattcatcg atgaattacc
    46321 aaaaggatat cactcaaaag tgatcgaaca tggtgccagc tattctagtg gacaacgaca
    46381 gctgatcact tttgcgcgaa caatcgtgac tgatcctaaa attctagtat tggatgaagc
    46441 aacagcaaat atcgatactg aaacagaagg attgatccaa gaaggattgg caaaaatgcg
    46501 tcaaggacgg acaacgattg cgattgccca tcggctgtca acgattcgag atgcagaatt
    46561 gattttagtt ttagacaaag gtcatatagt agaacgcggg aatcatgaaa cacttctagc
    46621 gcaaaatggt ttgtatgcag atatgtacaa actgcaaagc agtggtatat aaaagatgtt
    46681 gtgacagtag aattttgacc taagcactag agtgaaaata aaacatagct tctcatattt
    46741 ttcatctgtg agagttagtg tcgtagtcac cagatttaaa aaaagattcg acagctagtc
    46801 agaagtgcct aatagccact aagaaagcgt tgacaaaagc atttgctttc gataatataa
    46861 agaaaacgat acaacttgga tagtgattgc ctgatgcaag ctaaacctga tgagtctccc
    46921 atggtttcat ggacggcttt ttgggttttt ctttttgttt tggatacagc cctatacatc
    46981 atggaaagga gcaaaacatg ctgattgaga aagtactgaa taacaacgtc gtaataacca
    47041 gggatgaaag aaatcaggaa atgatcgtga tgggaaaagg tttggcattc aaaaagaaag
    47101 tgggtgaccc gatttttgac tcatgcatcg acaaaaaatt tcagttgaca aatgacgggc
    47161 tttcacaaca gttccaagaa ttggcacaag cgattcctct tgagtactta gaaatttctt
    47221 gtgaaatcat tgaatataca aaagaggctc tacaagtcca gttgaatgat tctgtgtata
    47281 ttgcattgac ggatcatttg tatacggcta tcgaacgtca caaaatggga atatttgtac
    47341 caaatgtatt gctgtatgat atcaaacaac tatttcctaa agagtatgcc atcgggaaaa
    47401 aaacagtgaa gaaaatctcg gataaatacc acatcaagtt atcagaagat gaagccggat
    47461 ttatcgcatt gcatttagtc aatgcacaag gcattcaaag ccagcaagac aatacctacc
    47521 tgtttactat aacaatacag gatattctaa atatcgtccg ctattactat aagattacat
    47581 ttgatgaaga ttcagtccat ttttttcgat tcgtcacaca cttacgtttt tttctgacac
    47641 gcgtcctaga tggagaaaaa tcagaagtgg gagaagtttc tgaggaattg tttcaggtta
    47701 ttcaaaaaaa atataaggat acgtttggtt gcgttgagaa aatagcagac tttttagccg
    47761 agacgttcaa ctaccagatg tccacggacg aaaaaatgta tttaacgatc catactgcgc
    47821 gtttagtgca tgagaatcgt tgtaaataaa aaaaggagga taaattaaca agaacgtatg
    47881 aaataagggt agtgacattc agttggctaa accttccact aataaattgg agggaataga
    47941 aatgggaaaa taccaagaat tagcgagaaa aatcgtagaa aatgtcggcg ggaaggaaaa
    48001 tatcaatggt ttgacacatt gtatcacaag gcttcgtttc aaattaaaaa acgaagaaaa
    48061 agcaaatgat gagatcttga aaaatatgga cggtattgta acggtaatgc gagcgggagg
    48121 gcagtatcaa gttgtcattg ggaatcatgt gccagtcgtg tttgaagagg ttataaaagc
    48181 aggaaacctt acttttgatg aggctgtttc aacagaaaaa atgagaccgt ttgatatgct
    48241 tattgatgga atatcgggct gtttccagcc attccttgct atcctagctg ctggcgggat
    48301 gattcgtgga ctgacagcat tccttgtctt cttaggcgcg tttgaccgcg gttctggcac
    48361 atttgttatg tttgataata tcggtgattc tgtattccaa tttatgcctg ttattatcgg
    48421 tttaacagct gcccgtaaat ttaaagtaaa tgaattcgta gggatgttgc ttggcgcagc
    48481 attaatgaat cctagcttgt cgctggaagc tttgagtggt gcagcagaag caccactaac
    48541 gaccatattt tctggcacta tttttgaagc acctatttat caaacagtat tcggcattcc
    48601 ctggattgcg cgtaactacg cttcttctgt cattcccatt atctttattg tgttgttagc
    48661 tagccaaatt cagaaaccaa ttaaaaagtt agtacctgag atgatcgcga atttcttcgt
    48721 tccattcttc accgttctta ttacaatgcc tttaggtttt ttactcgttg gaccagtatt
    48781 cacattcgca actgatattt taatggctgg atttgaaaca cttttagcac tttcgccagt
    48841 tatttatgga gcaatcgtag gcttcttctg gcaaattctg gtgatgtttg gtctccattg
    48901 ggcaatcgta ccaatgggat tgatgcaatt ttctgtgaat ggttggcaaa atattatgac
    48961 acctgttgca gtagtgtctt ttggacaaac tgcagcactt accgcactct actttaagct
    49021 tagaaatcca aaagacaaag caattgcaat accagccatt gtttctggta ttgtggggat
    49081 tacagagcca gctatttatg ggtttacatt accacgtaaa aaaatattta tttttacctg
    49141 tgtcggtggt gcgttagggg gcgcgtatag tgggttaatg aatttaactt cttggaatca
    49201 aggtggacta ggcatcttta caattcccaa ctatattaga ccagatggtg atttaacaga
    49261 tgtgattaat gtattgattg ggatcgccat tgcgatggtg gtatccttta cgctgacttt
    49321 tttcttttgg aaggatgaag caggtgaaac agatgatata cagaaaaaat caggtaaaga
    49381 aattgtaaaa acaccgatac aaggacaaat cgctccacta aatgcagcaa aggatgcagc
    49441 ttttgcacaa ggaacactag gtcgaggaat cttgatctac cctgaaaaag gagaagtacg
    49501 tgcccctttt gacggaacag tcatgaccct gttcccgacg aaacaggcaa ttggaatggt
    49561 ttctgaaaca gggctggaat tgctgattca tgtcggattg gatactgtgc agttggaagg
    49621 gaaatatttt gaaagcttag tgcagcaagg ataaagagtg aaacaaggcg atctgttgct
    49681 acgcttcgac aaaaagaaaa tcgaaaagga aggatacagt ttagaaacac ctgtgatcgt
    49741 caccaattac acggattatt tggatatttt ggaaaaccaa ggggagacgg tcgggccaga
    49801 cgatgcactt atcaccgcac tgacctgaac ggttaggtga agaatggact aggcttaact
    49861 attcttttta ttccacttct ttattttcaa aattgaaagg ctctttcatg atggctgagc
    49921 tgatagggcg tttatcgaat ttttgaccga cagaaattag atggactaaa ttctgttgat
    49981 aattttctgc aaaaacacaa ctatataaca cgtctagaag ataacaaatt gatgtattga
    50041 tcgtaaaatt accaattttt gaatacagtc gctcgcgtgt cgtaagtcgc aaagcggctg
    50101 tactcattgc agatagcgtt gaatcaccga tactggtcaa agctaaaatt ggaacatgat
    50161 tttgtttcaa tatttctgcg gtacgaagga tcacattgtt ttcacccgta taagaaatca
    50221 gaatcccgca catatcactc gtgcaattgt acgcttcata gaatgattca ccaaaagtgg
    50281 tgacaatcgt gacatccatt ttgatgcgct tcattttcag catgaaatct tgagaaatca
    50341 aagtattcga gttactggcg aaaatcacga ttttttttgc ttgaagcatt agttgtttgg
    50401 ctttttgcag ttggtcatga tgaatgagag aaagtgtatc atgaatcgtc atctgttcta
    50461 aagaagccat tttatttgcg atcgtcataa taccgtctgt ggtggaaaaa ggaagattcg
    50521 catctacgtc ttgaaaattc ccttcaagat aggctgtttc agctaaaaaa gcattacgaa
    50581 aactgaccca gccatcaaaa cccatttttt ttgctacacg gatgagtgta gaggggtgga
    50641 cataacatcg ctcagcaatc gcttggatgg tcatttcgcc aagtgctgga gaatcgctta
    50701 agagaaactg gatcacttca ttttctgaag gagaaaagga gatttctttg attttttcac
    50761 gtaataacat gtagatacct tcttcttatt tttttacgta aattataact tttttgaaaa
    50821 tgatttgtaa acggatttat actatataac ttaaaataga cattttttga caagatattt
    50881 acaaaaatcc actagaaaac agtaaatttt gtaaatgtta ctggaaagaa acagatttct
    50941 attgtttgtt ttgtttacaa aaaagataat gaaagtgtaa aaaagtaaag gagtgagaac
    51001 atggcaaaag gaatcaaaat cgctacgatt ggtggaggca gcagttacac ccccgagttg
    51061 atggaaggat ttatcaaaag atatgaggaa ttaccgatac gagaaatctg gctcgtagat
    51121 gtagaagaag gaaaagaaaa gcttgagatc gttagtcaga tgtctcagcg tatgtgggat
    51181 gcttctccgt atgaagtcaa gatctatgca actctggatc gggaagaagc attgaaagat
    51241 gcggattttg ttacgactca gtttcgtgtc ggtcaattag atgcacgaat caaagacgag
    51301 cggatcccta gttattatgg gatgattggg caagaaacaa atggtgctgg cgggatattc
    51361 aaagcttttc gtaccgttcc agtcgttttg gcaatcgttg aggacatgaa gcgtttatgt
    51421 ccaaatgctt ggctgatcaa tttcgcgaac ccaagtggga tggtgacaga agcagttatc
    51481 agatatggca aatgggataa agtgattgga ttatgcaatg tgccagtgat ggcgatgatg
    51541 acagaaccag caatgcttgg caagaaaaaa gaagagctga tctacaagtt tgccggtcta
    51601 aatcatttcc attggcacaa agtagcagat aataaaggaa atgaccgcac ggcagaattg
    51661 attgatcgtt tatacgatga agagaatgga ttaccgaaaa atatctttga tatcccgttt
    51721 ttcaaagaac aattaaaaca aatgaacatg atcccttgtg gctaccaccg ctattactat
    51781 cgtgaagaag aaatgctgga acatgcttta gaagaatatc aaacgattgg tacaagagcg
    51841 cagcaagtga aagaaacaga agcagagcta tttgaattat acaaagatcc agaattagac
    51901 cataaacctg aacaattgca aaaacgaggt ggcgcttatt attcagacgc tgcctgtgaa
    51961 acgattgctt caatctatgg aaataaagaa acacagatcg tggtatctac aaagaatgat
    52021 ggagctgttc ctgacctgcc ggcagattgt gtagtagaag tcactgccta tgttggcgca
    52081 caaggagcga gaaatgtagc gtttggttct cttcctgctg ctgaaagagg ctggttgcaa
    52141 gtaatgaaga atatggaatt gctgacgatc gaagcagctg tgactggtga ttatgggaca
    52201 gctttacagg cgtttacgat caatccactt atacgatcag gaaagacagc aaaacggatc
    52261 atggatgagt tgtttattgc acaccagcag tatttaccgc aattcaagga gacaatcagc
    52321 cgattaaaag aagaaggaat cgaagttcaa gatgaactgg caagagaatt atcaggtaat
    52381 acacatataa tggaggaagt ataatgggat tcagaaaaga ttttttatgg ggcggtgcta
    52441 cagcagccaa tcaatgtgag ggcggttatg atgaaggtgg acgcggacta gcaaacgtag
    52501 atgttgtgcc aatcggaaaa gaccgttttc ctgttatcac cggaaagaaa aaaatgtttg
    52561 aatttgatga ggatcatttt tatccagcaa aaattgcaat cgatatgtat catcgttata
    52621 aagaagatat tgctttgttt gcagaaatgg ggttcaagac ttatcgaatg tccatcgcct
    52681 ggagtcgtat ttttccaatg ggggatgaga aagagccgaa tgaagctggc ttgaaatttt
    52741 atgaggatgc ctttaaagaa tgccaaaagc acggaattga gccacttgtg acgattacgc
    52801 attttgattg tccaatgcat ttgatcaaag aatatggtgc gtggcgttcc agagaactcg
    52861 ttggttttta tgaaaatctg tgtcgagtca tcttcaatcg ctataaagga cttgtcaaat
    52921 attggctgac attcaatgag atcaatatga tccttcatgc accattcatg ggcgcagggc
    52981 tgtattttga aaaaggagaa aatgaagaac aaatcaaata tcaagcggca catcatgagt
    53041 tggttgcaag tgccatcgca acgaagattg ctcatgaagt agatccggag aacaaagtag
    53101 gctgtatgct ggcagcagga gcatattatc catacacttg caatcctgaa gatgtttggg
    53161 cttctagaaa atcagatcga gaaaattatt tcttcattga tgtccagtca cgaggagaat
    53221 atcccgctta tgcattgaaa gaaatggagc gaaatggcat tcagatcgaa atggaaccag
    53281 gggatgccgc attgctgaaa gaacatactg ttgattttat ctctttttct tattatgctt
    53341 cccgagtttc gactaccgac aacgaactct tggaacagac tgcaggaaat atctttgctt
    53401 ctgtcaaaaa tccatatctg caagcaagtg aatgggggtg gcaaattgat ccattaggct
    53461 tgagaataac gatgaatgat atttatgatc gttatcaaaa accattgttt attgtagaaa
    53521 acggcttagg tgcagtcgat acgccagatg aaaatggtta tgtggaagat gattaccgaa
    53581 tcgattacct ggcagcgcat atccaggcga tgaaggatgc agtagaacta gacggagtcg
    53641 atctattagg ctatacgact tggggatgta ttgatttagt atcagctggt accggtgaga
    53701 tgaagaaaag gtacggattc atttatgtcg atcgtgataa ccaaggaaat ggcacattag
    53761 cccgttcaaa gaaaaaatca ttcaactggt ataaaaaagt aattgattca aatggagaaa
    53821 atctttcgaa cgattgatca atgatttata aaagtctatt gtttttcagc ggatttttcg
    53881 agtataataa agctattcga gaaacgcaag cagaacaata gacttttttt cgcattggct
    53941 gtaaagtatg cttacttgat taagtaaagg agaatctaca tatgtatgat gtaatcgtta
    54001 ttggcgcagg tcctgctggg atgactgctg ctttatatgc ttcacgttca aacttatccg
    54061 tattgatgat cgaacaaggt gcccctggcg gccaaatgaa caatacggca gaaatcgaaa
    54121 attatcctgg attcgattcg atcatgggcc ccgatctagc ttataagatg tatgaaagtg
    54181 tctcaagatt tgggacagaa aatgcttatg gaatcgttca agatatcaag gatcacggca
    54241 gccataaaga agtcatctgt gaagataaaa gttacgaagg aaaaacagtc attatcgcaa
    54301 caggatgtat ccaccgtaaa ttaggcgtac ctggcgagga agaatttgct ggacgaggcg
    54361 tttcttactg tgcagtctgt gatggtgcgt ttttccgtaa caaacgtcta gtagtcatcg
    54421 ggggtgggga ctcagcagta gaagaagcca tctatttgac acaatttgct tctgaagtag
    54481 tgatcgtcca ccgtagagat gaattacgtg cacaaaagat catccaagac cgtgcatttg
    54541 caaatgaaaa aatttccttt atgtgggata cagtcgttga cgaaatcgtt ggaaacgaaa
    54601 tggtagtgac aggtgtgaaa gcacgtaatg tcaaaaccgg caatgaattt gatttagcag
    54661 cagacggagt attcatctat gttggactgg atccattaac tgcacctttt agagaagcag
    54721 gtttgacgaa cgaagctggc tggattccaa cagacaacga aatgaaaacg aatatgccag
    54781 gcgtttttgc catcggagat gttcgtgaaa aagatttgcg acaaatcact acagctgttg
    54841 gtgacggcgg tattgctgga caacaagtgt ataaatactt ggagtcttta gaagaaacaa
    54901 cagcataagt ttaaaaagag tagaagaacg ggttcaattg ttcttttact cttttttttc
    54961 gcgtgaaatt tactgaatct gatttatgcg aacacgtcca acaccttttg ataaaagaat
    55021 cctttcttga tttaagcgtt ttttggtcct catactttca tatctaataa gtgaatgcta
    55081 tacttattaa gaaccatatt tcagaaagag gtgttatttt catgtcttgg gaacaagtgt
    55141 atgaacaatg ggtacatgaa gaaaatttag aagaaaactt aaaaaaacaa ttaaatgatc
    55201 tgggtgaaga tcctgaaaaa cttgaagatg ctttttatgc accgttggaa ttcggtactg
    55261 caggtatgcg gggaatctta ggacctggga tcaatcgaat gaacatctat acggtaagac
    55321 aagcaacaga aggactagca cgtttcatgg atacacaagg tgaagaaaca aaacgccgag
    55381 gtgtcgctat cgcttatgac tcacgccatc agtcaccaga atttgcaatg gaagcagcca
    55441 aaacactagc acatcacaat atcccgtctt atgtattcga aagtctgcgt ccaacaccag
    55501 agctttcttt tgcagttcgt catttgaaga cgtttgcggg aatcatggta accgcatctc
    55561 acaacccagc ggcttacaat ggatataaag tctacggaga agatggcgga caaatgcctc
    55621 ctgcagacgc agatgcattg acgaaatatg tccgtgaagt cagcaatccg ctaaaagtcg
    55681 aagtattatc tgacgaagaa gctaaacata gcgggttgat cacgatcatc ggagaagaag
    55741 tggacgctgc ttatctagaa gaaatcaaag cagtgacgat tgatcgtgaa ttggttgaaa
    55801 caatggggaa agatttgaaa cttgtgtaca cgccacttca tggaacaggg aaaatgttgg
    55861 gcgaacgtgc gttgaaacaa gctggattcg aacaattcgt actagttccc gaacaagctg
    55921 tggcagatcc agatttcact acggtcaaat cgccaaatcc agaagaacat tcagcttttg
    55981 aatatgctat acgacttgga gaaaaagaag gagctgactt gctgattgca acagatcctg
    56041 atgcagaccg tttaggcgca gcggttcgtt tgccagatgg ttcttatgaa gtattgactg
    56101 ggaaccaaat cggagcattg tttgctcaat atattttgga agcacatcaa tcaagaggca
    56161 ctttaccaga aaacgcagcg gtattgaaat caatcgtttc cagcgaattg cctacagcaa
    56221 ttgcggaaca ctacggtgct accatgttca atgtgttgac agggtttaaa ttcattgctg
    56281 aaaaaatcca acaatttgaa gaagaccatt cacatacttt tatgtttgga ttcgaagaaa
    56341 gttatggtta tttagttaaa ccttttgttc gcgataaaga cgcgatccaa gcattgcttt
    56401 tattagcaga agtagcagct tactataaaa aacaaggcaa aacgttgtat gacggcttgc
    56461 aaacgatttt tgaaacctat ggctatttcg cagaaaaaac gatttctgtg acaatgagcg
    56521 gacaagaagg ttctgcgaaa atctctgctt tgatggaaaa attccgtaaa gaagcaccga
    56581 cagaatttgc cggagcaaaa gttattcaaa cagaagactt taaagaattg acgagaacag
    56641 accgtgaagg aaacgtagaa aaactaacaa caccgccatc cgacgtattg aagtatacat
    56701 tggaagaaaa tggctggatc gctatccgcc cttcaggaac tgagccgaaa atcaagtttt
    56761 atattggtgt aaaaggaagc tctacagaag acgcagccga aaaagtagcc tcttatgaag
    56821 cgattatcaa aaagatgaca gaagaataaa aaacttgaga ctgatggaaa aagtggtaaa
    56881 ctttagaagt cagtcttttt tcaagttgga ggtgaatgga ttgccagaca cgcaaaaatc
    56941 aaaagtagag aaagtcagtc aattattcaa aatgttgagt gatcccactc gtcttaaaat
    57001 tctgctatat ctgaaagacg gggaacaaaa tgtgacagca atcacccaag cagtggaaat
    57061 ggagcagtct gctgtatcgc atcaactacg tttattaaga gaaaatcatg tcgtcaagtc
    57121 tcatcgcgag ggaaaagcga ttttgtatag tcttgatgat tctcatgtac tggatatttt
    57181 gaaccagaca ctcaaacatg tggaacatca ataacgatag gtgaaaatag ataaacactg
    57241 gagtgtgaca acgaagaaat ttcgttgtca cactccagtg ttttttaaga aaaagacaaa
    57301 aatgattaat cgcttttttc tgttgtttca gttccataat aactatgatc ttctaggtct
    57361 aaagcattca aaataagcga agccgtttct tcaatagata aagatgccac attgatcact
    57421 acacatccta gtttttcata aagatcattc gcaaaatcga gttcagctct aattttttcg
    57481 atatcggaat aagcagtatc agggtttaat ccgtaagagc gcatccgttc tttacggata
    57541 ttatttaaaa tatctgggtt attcgttagt cccacgattt ttttaggatc gatttcccac
    57601 agctgtttgg gaatatgtgc ttggggaatc aaaggtaaat tagcaacttt taaattttta
    57661 tttgctaaaa acaagctcaa aggcgttttg gaagttcggg atacgcctag caaaacgaca
    57721 tctgcttcta aaaaaccacg aggatctttc ccatcatcgt attttacagc aaattccatt
    57781 gctttgatcc gtttgaaata gttctcattc aataaatgga gggcaccagg ttgccgaatt
    57841 ggcttgatac cagttaaacg ttcaatttct gatatcggtg gcgtcatcac atcgaaacta
    57901 aataattgat gttcttcaca aaaagaacgt gttatctcga tcagttcttg attgattaat
    57961 gtatgcagga caagtgcttt ttcttctaat gcatcttgca atgcctgcct taatgttttt
    58021 tcatctgtca cgaacgttct gcggaatagg ttgaactcaa cggtaggata ctgagccata
    58081 gtagcttgcg ctagttttgt cgcagtttct ccagcagagt cggagatgac aaaaaaagta
    58141 acgatttctg tagaatcgga agacatggat aaaacctcac ttgcattttt tttatgttta
    58201 ttataccata ctctagaatg cgtacttcgt aatcattttg atttggagga ataagtcatg
    58261 aaaaaacaga cattgatcga acaatcatta gaaacattga aaaataacgg ttttaaatat
    58321 actaaaaaaa gagaagcatt gttaacttat ttagtgaagc gtaaccgcta cgtatctgct
    58381 aaagaagttt ttgactatat gaatcaggaa ttcaaagggg taagttatga tacgatttac
    58441 cggaatctcc atgattttgc agagatcgat ttactggaag aaaccgattt aaatggtgaa
    58501 atgaagtttc gttttcattg ctgtcagggg gaaatcggcc atcaccatca ttttatctgt
    58561 acagtgtgcg gaacaacgaa agaaatccat atgtgtccaa tgaattattt cgaagaacag
    58621 ctaggtggat gcacgatcga agggcataga ttcgagattt tcggaagatg tgaaaattgt
    58681 caataaggat ttaaataaaa taacatttga taatttaaaa aaaaagcaag atttccttga
    58741 cggtcaaaac agcattagat ataatattta caggacagtc ttgttttttg agatatattt
    58801 caataacaat tacggttact ttttaagctc ggagggaggg aattaacatg tcaaaaacag
    58861 tcgttcgcaa aaacgaatca cttgacgatg ctcttcgtcg cttcaaacgt tccgtgtcaa
    58921 aagcgggtac tttacaagaa tctcgtaaac gtgaattcta cgaaaaacca agtgtaagac
    58981 gcaagaaaaa atctgaagca gctagaaaac gcaaaaaatt ctagttctgt gatgaattgg
    59041 agttggtagt gtgtcactac taagtagatt aaacgatgac atgaaagcag cgatgaaagc
    59101 gaaagataag gaatctttac aagtcatccg aatgatcaag tcatctatac aaaacgagca
    59161 gattaaagaa ggtcacgact tgacagaaga agaagaacta acggtgttgt ctcgcgaaat
    59221 gaaacagcga cgtgattctt tgcatgagtt tgaagaagca ggacgtgacg atttagctga
    59281 aaaagtaaaa agtgaaattg taatagtcga aaaatatatg ccggaacaac tttccgacga
    59341 agaaattcga caacttgttc aagaagcaat cactcaaact ggtgcttcat caatgaaaga
    59401 atttggcaaa gttatgggtg caattatgcc taaagtcaaa ggaaaagcag atggaaacca
    59461 agtgaatgcg attgtgaaag aactattaca atcttaattt tatcatttaa actgcggaca
    59521 gtgattgtcc gcagtttttt gtgttcgtaa aaaaagaact aaaaaacagc tagaagaaag
    59581 aataagattc tttcttctag ctgttttttt cctgtttaat atctcttatg ttaaattgtg
    59641 tttgatttgg ttattcttct ggtagttcat ttgcttcttg tttataccct gataggcgat
    59701 aggcgtaata aaagagtaag ctgccagcgt aaccaagtaa cgttgggacc atccagccaa
    59761 gtcctaatgc agcaaaagga acgtgatgat gataaaatcc tagtaagctt gctacgacag
    59821 tttgctgact cactaatgca ggcgaatttg ccaacatatc caatacggct ggaacggcag
    59881 aaaagaacat cgttacttga tagacaaatg gactcttttg gaagaatttt gctgttaagg
    59941 aaagcaagat taacgcaagt gacaatggat acaaaagcat caatactggt aaagaccatt
    60001 ggatgatatt atccaatccg gcattcgcaa cgacaaaaga gacaaaagtc gttaaccgca
    60061 gccatgtcat ataactgact ttagggaaca gtttatggaa atcttgtgca aaagaaacaa
    60121 cgagtcccat agcggtagtg aagacaccta atgtgaccat tacccctaag aagctagcac
    60181 cgaagttacc taaataaaaa ttagtgatct gagaaagtgc agttccgcca ttttctgata
    60241 aaggcatctt attcaaactc atcgcaccga gaaggaccag accaaaataa atcatgactt
    60301 ctaacgcaac gctcaggcta ccagcttttg ctgttaattc tgtgactttt ttatctttaa
    60361 aaccaagatc acgaactgca tggacaaaag tgacacttaa agcaagtaaa gctactgcat
    60421 caaccgtgtt gtatccttct aaaacgccat ttgtcaaagc gttcgtctga taagaatctg
    60481 cagcagattg gtttagattc cccatgggtt ggaataaaga aacgacaaaa atgatagcta
    60541 ataatgcaag aaacaaggag tttaaatact ttccgacata tttgaccaaa cgtgttggat
    60601 gtacagtaag gaaataagca agtaaaaaga aaagagcaga gaataaaaac atacctaatg
    60661 aatcatattt ttcaggcaaa aagggtttag cagccatttc aaaagcagtt gctgctgtcc
    60721 gcggtgtacc aaagaatggt ccgatcgtta aatgaacaag gatcaagaac aaggcagcat
    60781 accattttcc gacaggttta gctaagtcat acagaccatc actttttgtc actactacag
    60841 ctaagatagc taataatggg aaaagagcgc ctgagatggc aaatcctaca ccagcgctga
    60901 tccagtttga tccagccatt tgacctaagt ggacaggaaa gatcagatta cctgacccga
    60961 agaacatccc gaagataagt gaactgacca tcaataattg cttccatgaa taagtttttg
    61021 tttcttccat aaatttttcc tccaataatt atcattttga gtaacaaaat aaacgttgca
    61081 gaaactagaa atgccactaa tatcagattc catgaaaacc accaccttaa aatataaaaa
    61141 aaagcccttt tccacttggg aaaagggctc tagattctag aacggtacca ccttttttcg
    61201 cttaaaaaag cttcttcatt cacggacgct tttcgataaa gttatccgct agcctgataa
    61261 tggaggcagc cacaatgatc tacgttttcg acattgactc ggaaatgatg ttcgtctatt
    61321 cgatttattg tcatttctca cttacatgac tcactgggaa taaatcgctg aatagcctac
    61381 tgttttcctc gacgctttgc tgtatattta tttgttgacg ttataataca cctgtttttt
    61441 ttgaaatgca agaatatttc aaatattttg ttttcttttt ttgaaacaag aaaaataccc
    61501 agagaatact tgctaagtta gacggaatac ttgtcctcta tcttatgcat agcttacatg
    61561 atcagctttt tttattcaaa cgagaccttt agagaatgga agcagctatt ttttaacgtt
    61621 tcttggtact tatggttaag caactctttt agaacctttt cttatttgct atatagtttc
    61681 ttctgagtat ggtaaaatag gaaagaatga atcgaaaaaa aggggatgtc tatttttgac
    61741 ggaagagtct agttctttag agattagatt gacggctgct gatgatgtca gcatgctttt
    61801 aggtgcacac gacaagcata taaaactgat tgaagaaaca acagaaacaa ccatccatac
    61861 acgtggagaa atcattcaga tcattggcga acaatcccag atttccttgg ctgcctcagt
    61921 cattcggaca ttgcaggaat tgatcaaacg aggaatccat gtaagtacac cagatgtcgt
    61981 gacggcactg aaaatggggc aaaaaggcac attagattat tttgttgaaa tgtatgagga
    62041 agagatcgtc aaagaccgta atggaaaacc aatacgggta aaaaacagcg gccaaaagaa
    62101 atatgtcgaa tcggtagcca aacatgatat cgtatttggc gtaggaccag ccgggacagg
    62161 aaaaactttt cttgcagtag tattagcaat cgctgccttg aaaaaaggtg aagtgcaaaa
    62221 aatcattttg acgcggcctg ctgtggaagc aggggaaaat ctaggctttc ttcccgggga
    62281 tctaaaagaa aaagtagatc cttatctgcg tcctgtttat gatgctttat accaaatttt
    62341 tggattagat catacgaacc gattgatgga acgaggcgtg atcgaaattg ctcctttagc
    62401 ttacatgcgg gggcggacat tggatgatgc attcgttatt ttggatgaag cacagaacac
    62461 gacagttgct cagatgaaaa tgttcctgac acgtcttggt tatcaatcaa aaatgattgt
    62521 caatggagat acgagtcaga ttgatctgcc tcgagggaca acaagtggtc ttgttcatgc
    62581 agaacgaaca ttaaagcaaa tcaagaaaat cgattttgtt aattttgaag caagtgacgt
    62641 tgttcggcac cctgtagttg ccgaaatcat caaagcttat gaaaaggcag atctccatca
    62701 agaataagct ttgcttagga ggataagcac atgttgaata aaacactgca gaaactacaa
    62761 gaaaaacttg gaaaggcagc attgcctttc ctgctgttct tgttttttat cattatctgc
    62821 ggcgttacat ttagtagtgt caaacaaaag gccaatgatt tcagagaagg ccaagtagca
    62881 gaagaaagta tccgtgcgaa caaaacgatc gagaacacgg aagaaaccga acaaaaaaga
    62941 aaactggcag cagaagcagt cactccagaa tacacttacc aaaaagatct tgcagatgac
    63001 cagaacaatc ggatcaagca actatttgaa ttgatcgata aaacgaatga tgccatcaac
    63061 aaatcttatg acgaaaagat tgataaggca aaggaagatg aaaatatccc aaagcctact
    63121 acagaagagc ggatcgccgc gttgaagaaa aattttgaaa atgtgaatgc agaaaatgtt
    63181 gcgttttatc aaaaactacc gaacaatttt tattcgacga tatttgaaat gactgaaact
    63241 gagatccata ctgtgaggga tgagagtctg aagctcattg acgaacagat gagcaaacaa
    63301 gttcgtgaaa gtgaattgga agcttttaaa caagaagcag aagagcaaat ccaatatttg
    63361 aatgtcacac cagcacaaca acaaatgatt cgctacttag tggatcaagg tatcgtagtg
    63421 aatgacgtat tgaatgagaa aaagacagaa gaattgaaac aatccgcacg agaagctgta
    63481 caaccagtga tgatcttcca aggagaaatc attgttcgtg aaggtaatca aatcgatgct
    63541 tcagctatga aaaaattgga attactagga ctgactaatc aaacaacttc gatttttccg
    63601 ctaattgcca tggttttagc tgttttgctt caaatcggcg tactgattta taattcactt
    63661 caattccaaa atgcaggaga aagaacaaaa tacgtgcttt tctatgtgac agctatgtca
    63721 gtcagcatag tgctgatgaa atttttccaa ttattccaaa cggaacaagc tacttatatc
    63781 ccgctgtttt atcccgctgc ttttgcacca cttgttttaa gctttttctt gaaccgaaga
    63841 gcagggatca tggctggtat tttccaagca gtatctgcac ttttcatttt ttatgattcg
    63901 attggcacga actttctaac gattatcttg atgtcttatg ttttctctgg gctcatggca
    63961 acgattgtca gacgcaaacg gatctcagaa caaggattat ctgctgccat gtgggtcatt
    64021 atttttcctg tgatgatgga tgtcattttg atcgtctatc aaggaatgag ctttggtgat
    64081 gcaacgactt ggttaatgct tgtctgcggg ttcactggtg cactcttctc attcttgctg
    64141 acgatcggtc tacacccata tattgaattg atggtcaatg atgacagtgt aattgtatta
    64201 aatgaactca gcaatccaaa tcatccttta ttgaaacagt tgctggaaga agcgccaggt
    64261 acttatcatc atagtatgat ggtagctaat ctaagtgcta acgctgtagc agaaatcggc
    64321 ggacgttctt tgctaactcg agtagcttgc tattatcatg atatcggcaa gatcaaacat
    64381 gctagctttt tcgttgagaa cttgccttca ggagcggaaa acccgcacaa tttcttattg
    64441 ccagaagaca gtaaacatat tatctttgga catgtgacag atggagccaa aatattggaa
    64501 gattataata tgccgcaaat ggtgatcgat atttgcaaac agcatcatgg aaccacgcta
    64561 atgcgttatt tctacatcaa agcgaaagaa cgcaatccgg aagtaacaga agagcaattc
    64621 cgttatcctg gaccaaaacc gcaaagtcgt gaagcaggga tcgtcaatat tgctgacagt
    64681 tgtgaggctg cagtacgggc aatggatcat ccgacgagcg aaaaaatcga aagttttgtc
    64741 cataatctta tcgaagagcg tatttccgat ggtcaattag acgatagtgg actgaccttg
    64801 aaagaaatcc gaaaagtgga aaaatcattg atcagcggtt taagtagcac cttccattct
    64861 cggatcaaat atcctaaaat gaaaacagaa gttgagaaga taaaggaaga acaagaagaa
    64921 tagaaagaag cgatgtataa tggatattac ctttatggat gaaacaaacc aagtctcaga
    64981 agagaaaatg aaagaaatcg atgacttgct gcaatttgca gcatcttatt taaagctgcc
    65041 agaggaaaca gaaatgtctg ttactttcat ggacaatgcg gcaatccaag taatcaatcg
    65101 tgattaccgt ggaaaagatg caccgacaga cgtcatcagt tttgctctgg aagaagaagg
    65161 cgaagacgaa atcccagtga tttttgaaga tggagagaat ccattgcctc gtgtactagg
    65221 agacatcatg atttctattg aacgtgctgc tgaacaagca gaagcttacg gccattcata
    65281 tgatcgggaa cttggctttt tagcggtaca tggattcttg catatcaacg ggtatgacca
    65341 tatgacacca gaagatgaga aagaaatgtt tggtttacag aaagagatct tggatgccta
    65401 tggactcaaa agataaaaac gtagagaaaa acaaacattt cctgatgtct gtggaatttg
    65461 ctgtccaagg agtcaaaacc gtttttgagg aagaacgcaa tatgaggaaa catgttgcgt
    65521 tcggtatcct tgctttattt atgggcgtga ttttccagct tgatcggata gagtggttat
    65581 ggctgctttt agctgttttt ctcgtttgga tagtagagat catcaatact gtttttgaaa
    65641 atgtagtgga catgtttacg gattttcatt ttcatccgat tgggaaaaaa atcaaagata
    65701 tggcagctgg agcagtttta gtgaccgctt gttttgctgt catcattggg ttgattttat
    65761 ttgtccctaa aatatatcat ttgttgttta attaaaaagg agaacgtata tgccagaaca
    65821 taaatcaggg tttgtcgcaa tcgtcggccg cccgaatgta ggaaaatcca cattattgaa
    65881 ccgaatcgtg ggtcaaaaaa ttgccattat gagtgacaag gcacagacaa ctagaaataa
    65941 aatccaagga gtatatacta caccagaagc tcagctgatt tttatcgata caccaggaat
    66001 ccataaacca aagcatcgtc taggagattt tatggtagaa gcagcttaca gtgcgttgaa
    66061 agaagtggat gcagtcttat ttatgatcag tgctgatcaa aaacgcggcc ggggagacga
    66121 ttttattatt gaacgtctaa aaaacgtgca ttctccagtt tatctgatta tcaataaaat
    66181 cgataagatc catccagacg agttgctggg aatcatcgaa gattattcga cgcagatgcc
    66241 ttttgcacaa gtgattccga tctctgctac agaaggaaac aatgtcgaac gcttaatgga
    66301 tgtattagtc agtgaaatgc cagagggccc acaatatttt ccagatgatc aagtgactga
    66361 tcatcctgaa tactttatcg tttctgaatt gatccgtgag aaagtcttgt ttttaacacg
    66421 agacgaagtg cctcattctg tcgcagtcgt tgttgattcg atgaagcgaa acgagaatga
    66481 taaaatccat atccaggcca cgatcatcgt cgaaagagat agccaaaaag gaatcattat
    66541 cggcaaaggc ggaaaaatgc tgaagcaagt aggaacgaaa gcaagaaagg atatcgaaaa
    66601 tcttttagga gacaaagtat ttctagaact ttgggtcaaa gtccaaaaag actggcgaga
    66661 caaaaaagtc tatctgcaag actttggcta ccgaaaagac gaatactgat attctgaact
    66721 tcttataggt taagtataga aataaactgg ataaaaatcc gcacgttgag aaaaacggcg
    66781 gaagatcgaa acataaataa tctggatcgg cagaatctgc ttttccagat ttatttttta
    66841 ggagggagaa tatgagccag cttgcagaat cgaaaggctt gattttatct agccgtaatt
    66901 acaaagaaaa agataaatta gtcaaaatct ttacggaatc ttctggcaaa atgatgtttt
    66961 atatcaaagg aatccatcgg caaaatcagc cattagctcc ggctttgctc ccctttaccg
    67021 aagcagtcta tatcggtaat tttcgaagtg aaggtttgtc gtttttaagc agtgtcaaag
    67081 acgtccatgc gtttcggaac atccaagaag atatcttttt gagcggttat ggtacctatt
    67141 tgctaaatct agtagatgca gcaatcgaag atcatcaata tgacccgcat ttattccaat
    67201 ttacgcaaca agctctgcag cggatggatc aaggagatga tgcagaaatc atcacgaata
    67261 tctttgaagt tcaaatactg caacgcttcg gaatcgcgcc aatttggacg cactgtgtcg
    67321 tatgcgggga aacaaaagga aaatttgact attcttccaa atctggcggt gtgatatgcg
    67381 agaaacactg gccaatggac gaacaccgtt atcatgcaga tcctcgcgct gtttatttcg
    67441 ttcgaatgtt ttcggctatt tcttatgata agatcagtgg aatccacttg aaagaagaaa
    67501 cgaagcaggc aatccgtgac ctcattgacc agttgtatga tgaatatgtc ggtattcatc
    67561 taaaaagcaa aaagttcatt gatcaaatga agtcttgggc acacgtatta aagacgccag
    67621 agaaaaatat gcctgaaaaa gagatacctg aaaaagaaga ataaatgaaa gatacaacaa
    67681 aaaaatcaaa actatttgac aaatacaggg acttccgtct attattaacc gtagattaaa
    67741 tagatcgttc actgagagga tctattatat aaacaagaaa actgcaaaat gaatacgtac
    67801 attgaccaag atcagtaaaa aacacgtggt ttagcgagtt cgggagagtg gaagccggac
    67861 ccaaacgatt tttgaagagg cacttgcgag tacgataaat gaagctgccg aatctttatt
    67921 cggaagtagg gtggaaccgc gaaattattc gtccctatgt ctgtaatcag acatagggac
    67981 tttttgtcgt tttctttcct gtattcttat tgacagcaac gcttaaaaat tgaaaggggc
    68041 aaaacagatg agtaaaaaat tgaccgtcca agatatgatt ttaacattac aaaaattttg
    68101 gtcatcaaac ggctgcatgc tgatgcaagc ttatgacaca gaaaaaggag caggtacgat
    68161 gagcccatat acattcttac gggcaatcgg accggaacca tggaatgctg cttatgtaga
    68221 gccatcccgc cgaccagctg acgggcgtta tggagaaaac ccaaaccgtt tgtatcagca
    68281 tcatcaattt caagtcgtaa tgaaaccttc acctgaaaat atccaagagc tttatttaga
    68341 aagcttgcgt cttttaggaa ttgatccatt agaacacgat attcgttttg ttgaagacaa
    68401 ctgggagaac ccatcgatgg ggtgtgccgg tgtgggttgg gaagtatggc ttgatgggat
    68461 ggagatcaca caattcacgt acttccagca agtcggagga ctaccttgta aaccagttac
    68521 ttctgagatc acttatggac tagaacgtct tgcttcatat attcaagaag ttgaaagtgt
    68581 ctatgatctg gaatgggctg atggtgtgaa atatggcgag atcttcaaac aaccagaata
    68641 tgaacattcc aaatattctt ttgaagtaag tgaccaagac ttgctactaa gcaactttga
    68701 ccgttttgaa aaagaagcta aacgctgtat cgatgaaaat cttgttcatc cagcgtatga
    68761 ttatattttg aaatgcagcc atacattcaa tttattagat gctcgcggtg cagtatccgt
    68821 aacagaacga gcaggttatc tttcccgtat ccgcaacatg gctcgtgctg tagccaaaat
    68881 ctttgtggca gaacgcgaaa aattaggatt ccctttattg aataaaaaca cagcaaatcc
    68941 agaaaacatg aaggaggctg aataagatgg caaaagactt attacttgaa atcgggctgg
    69001 aagaaatgcc tgctcatgtt gtcacaccaa gcatgaaaca attagaagaa aaaacagcaa
    69061 aatttttaga tgaaaaccaa ttgacttatg atacgatcga aacgttttct acaccgcgcc
    69121 gcttagcaat caaagtaaca aacattccag aacgtcaagc agacagtgaa gaagaagtca
    69181 aaggaccagc caaaaaaatt gctttggacg cagatggaaa ctggacgaaa gcagctgaag
    69241 gatttgtccg cggacaaggt ctgacgacag atgctatcac ttttagagaa ttaaaaggcg
    69301 tggaatatgt ctatgtaacg aaatacactc acggcaaaga agcgaaagaa gtattgacag
    69361 gtttagcaga tgtcattaaa agcctcactt tcccagtaac gatgcattgg gcagaatacg
    69421 attttgaata tatccgccct attcactgga tcgtggcatt gttagatgac gaagtaatcc
    69481 cgtttgagat cttagatgtc aaaacaggca gagcctctcg aggacatcgt ttcttaggcg
    69541 aagatgtgac atttgcgcat gcaaaagaat atgaagaaaa attgaatgaa caattcgtta
    69601 ttgcttcacc gcaaaaaaga caagaaatga tcgttacaca agcaaatgaa ttagccgaaa
    69661 aaaatcactg ggttattgat ctagacgaag atttactaga agaagtcaac aatttagtcg
    69721 aatatccaac tgtcttttct ggcgacttcg aagaaaaata tctttctgtt cctgaagaag
    69781 tactagtcac ttctatgaaa gaacatcaac gttatttcga ggtacggaac gatcaaggta
    69841 tgctgcttcc tcattttatt tctgctcgta atggtgatcg ggtaaaattg gaaaatgtgg
    69901 ttcgaggcaa tcaaaaagta ttgacggctc gtttagaaga tgcggaattc ttttataatg
    69961 aagacaagaa agcttcaatc gaccaatacg ttgaaaaact aaaaactgtt actttccatg
    70021 aaaaaatcgg tagtatctat gagaaaatgc agcgggtaca agtcattgcc cgtatcattg
    70081 gcgaacaagt aggactatct gaggaagaat tgatggatct gcaacgagct gctgaaatct
    70141 ataaatttga tctagtaacc aacatggtag gggaatttcc tgaactacaa gggatcatgg
    70201 gcgaaaaata tgctctgctg aaaggcgaga aacctgctgt agcaacagcg attcgtgagc
    70261 attacatgcc tatatccagt gaaggagagc tacctcagtc tacagtaggt tctgttttag
    70321 cgattgcaga taaattagat agcatttttt ctttctttgc agttgggatg attccaagcg
    70381 ggtcaaacga tccttatgcc ttacgtcgcc aagcatacgg tgtcgttcgg atcatcgaat
    70441 cgaaaaaatg ggcctttcct ttgtctgttc tgcaagaaac gattagtgat gttatcagta
    70501 aagatacgga tcgtttcggc attggtttat cagaagggca acaacaggtg atcgacttta
    70561 tcaaaggccg tctgcgccaa ttattgacaa cgaagaatat tcgtcatgat gtcatcgaag
    70621 ctgttttgaa tgcagaacaa aaagatctga caaaagtatt tgcagcagca caattgttca
    70681 agcagcatct tgcagatgaa gacttcaaac catcgatgga agctttgaca cgtgtcgtga
    70741 acttagcgaa aaaagcggag cttgaaaaac aatcagaagt agaccctgaa ctgttcgaaa
    70801 atgaagcgga gaaagaactg cacaaagcag tagaggcaat gaaaaaaagc tttgctgacc
    70861 aaacgatcaa cgaaaattat gatcagcttg taagccttcg cccactgatc gaaaattatt
    70921 ttgatcagac gatggtcatg gcagaagaac cagcagtacg taagaaccga ttaacgcaat
    70981 taagtcaaat tgctgccatg gctttgtcgc tagcaagttt agatcaaatc atcacaaaat
    71041 aatttctaat atgtaaataa accagagaat aggcacaaaa
//
LOCUS       DAJSAW010000007        69518 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJSAW010000007 DAJSAW010000000
VERSION     DAJSAW010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 69518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.007"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..2387)
                     /gene="esp"
                     /locus_tag="ONT64_000537"
     CDS             complement(<1..2387)
                     /gene="esp"
                     /locus_tag="ONT64_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HCR2301976.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
     gene            complement(3004..3438)
                     /locus_tag="ONT64_000538"
     CDS             complement(3004..3438)
                     /locus_tag="ONT64_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301977.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3455..4660)
                     /locus_tag="ONT64_000539"
     CDS             complement(3455..4660)
                     /locus_tag="ONT64_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HCR2301978.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5354..5740
                     /locus_tag="ONT64_000540"
     CDS             5354..5740
                     /locus_tag="ONT64_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2301979.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5834..6025
                     /locus_tag="ONT64_000541"
     CDS             5834..6025
                     /locus_tag="ONT64_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2301980.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6539..6871
                     /locus_tag="ONT64_000542"
     CDS             6539..6871
                     /locus_tag="ONT64_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2301981.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7167..7322
                     /locus_tag="ONT64_000543"
     CDS             7167..7322
                     /locus_tag="ONT64_000543"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCR2301982.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7598..8938
                     /locus_tag="ONT64_000544"
                     /pseudo
     CDS             7598..8938
                     /locus_tag="ONT64_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9179..10795)
                     /locus_tag="ONT64_000545"
     CDS             complement(9179..10795)
                     /locus_tag="ONT64_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCR2301983.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            10913..11131
                     /locus_tag="ONT64_000546"
     CDS             10913..11131
                     /locus_tag="ONT64_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2301984.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            11326..11787
                     /locus_tag="ONT64_000547"
     CDS             11326..11787
                     /locus_tag="ONT64_000547"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2301985.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(11980..12219)
                     /locus_tag="ONT64_000548"
     CDS             complement(11980..12219)
                     /locus_tag="ONT64_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2301986.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(12243..13766)
                     /locus_tag="ONT64_000549"
     CDS             complement(12243..13766)
                     /locus_tag="ONT64_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCR2301987.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(13784..13906)
                     /locus_tag="ONT64_000550"
     CDS             complement(13784..13906)
                     /locus_tag="ONT64_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCR2301988.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(13936..14919)
                     /locus_tag="ONT64_000551"
     CDS             complement(13936..14919)
                     /locus_tag="ONT64_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR2301989.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(14943..15092)
                     /gene="rpmG"
                     /locus_tag="ONT64_000552"
     CDS             complement(14943..15092)
                     /gene="rpmG"
                     /locus_tag="ONT64_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR2301990.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(15113..15490)
                     /locus_tag="ONT64_000553"
     CDS             complement(15113..15490)
                     /locus_tag="ONT64_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2301991.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(15522..15701)
                     /gene="rpmF"
                     /locus_tag="ONT64_000554"
     CDS             complement(15522..15701)
                     /gene="rpmF"
                     /locus_tag="ONT64_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR2301992.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(15716..15985)
                     /gene="rpsN"
                     /locus_tag="ONT64_000555"
     CDS             complement(15716..15985)
                     /gene="rpsN"
                     /locus_tag="ONT64_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR2301993.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(16508..18274)
                     /locus_tag="ONT64_000556"
     CDS             complement(16508..18274)
                     /locus_tag="ONT64_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2301994.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(18234..19988)
                     /locus_tag="ONT64_000557"
     CDS             complement(18234..19988)
                     /locus_tag="ONT64_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2301995.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(20098..20667)
                     /locus_tag="ONT64_000558"
     CDS             complement(20098..20667)
                     /locus_tag="ONT64_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HCR2301996.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(20685..22100)
                     /locus_tag="ONT64_000559"
     CDS             complement(20685..22100)
                     /locus_tag="ONT64_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR2301997.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(22111..22779)
                     /locus_tag="ONT64_000560"
     CDS             complement(22111..22779)
                     /locus_tag="ONT64_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR2301998.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(22909..23493)
                     /locus_tag="ONT64_000561"
     CDS             complement(22909..23493)
                     /locus_tag="ONT64_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2301999.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(23824..24057)
                     /locus_tag="ONT64_000562"
     CDS             complement(23824..24057)
                     /locus_tag="ONT64_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HCR2302000.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(24072..25022)
                     /locus_tag="ONT64_000563"
     CDS             complement(24072..25022)
                     /locus_tag="ONT64_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCR2302001.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(25019..25861)
                     /locus_tag="ONT64_000564"
     CDS             complement(25019..25861)
                     /locus_tag="ONT64_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR2302002.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(25863..26582)
                     /locus_tag="ONT64_000565"
     CDS             complement(25863..26582)
                     /locus_tag="ONT64_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR2302003.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            27274..27432
                     /locus_tag="ONT64_000566"
     CDS             27274..27432
                     /locus_tag="ONT64_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2302004.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            27504..28730
                     /locus_tag="ONT64_000567"
     CDS             27504..28730
                     /locus_tag="ONT64_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2302005.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(28818..29210)
                     /gene="rpsI"
                     /locus_tag="ONT64_000568"
     CDS             complement(28818..29210)
                     /gene="rpsI"
                     /locus_tag="ONT64_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR2302006.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(29226..29669)
                     /gene="rplM"
                     /locus_tag="ONT64_000569"
     CDS             complement(29226..29669)
                     /gene="rplM"
                     /locus_tag="ONT64_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR2302007.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(30188..30655)
                     /locus_tag="ONT64_000570"
     CDS             complement(30188..30655)
                     /locus_tag="ONT64_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCR2302008.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(30775..32313)
                     /locus_tag="ONT64_000571"
     CDS             complement(30775..32313)
                     /locus_tag="ONT64_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR2302009.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            32393..33079
                     /locus_tag="ONT64_000572"
     CDS             32393..33079
                     /locus_tag="ONT64_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR2302010.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(33280..33807)
                     /gene="msrA"
                     /locus_tag="ONT64_000573"
     CDS             complement(33280..33807)
                     /gene="msrA"
                     /locus_tag="ONT64_000573"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR2302011.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(33891..34568)
                     /gene="pgmB"
                     /locus_tag="ONT64_000574"
     CDS             complement(33891..34568)
                     /gene="pgmB"
                     /locus_tag="ONT64_000574"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR2302012.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(34565..35695)
                     /locus_tag="ONT64_000575"
     CDS             complement(34565..35695)
                     /locus_tag="ONT64_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR2302013.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(35695..37878)
                     /locus_tag="ONT64_000576"
     CDS             complement(35695..37878)
                     /locus_tag="ONT64_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCR2302014.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(37875..38900)
                     /locus_tag="ONT64_000577"
     CDS             complement(37875..38900)
                     /locus_tag="ONT64_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2302015.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(38945..39772)
                     /locus_tag="ONT64_000578"
     CDS             complement(38945..39772)
                     /locus_tag="ONT64_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR2302016.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(39769..40836)
                     /locus_tag="ONT64_000579"
     CDS             complement(39769..40836)
                     /locus_tag="ONT64_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR2302017.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(40862..41698)
                     /locus_tag="ONT64_000580"
     CDS             complement(40862..41698)
                     /locus_tag="ONT64_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2302018.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(41695..42564)
                     /locus_tag="ONT64_000581"
     CDS             complement(41695..42564)
                     /locus_tag="ONT64_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2302019.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(42634..43917)
                     /locus_tag="ONT64_000582"
     CDS             complement(42634..43917)
                     /locus_tag="ONT64_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR2302020.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(43931..45616)
                     /locus_tag="ONT64_000583"
     CDS             complement(43931..45616)
                     /locus_tag="ONT64_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCR2302021.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            45891..46916
                     /locus_tag="ONT64_000584"
     CDS             45891..46916
                     /locus_tag="ONT64_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2302022.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(47511..51185)
                     /gene="rpoC"
                     /locus_tag="ONT64_000585"
     CDS             complement(47511..51185)
                     /gene="rpoC"
                     /locus_tag="ONT64_000585"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR2302023.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(51243..54860)
                     /gene="rpoB"
                     /locus_tag="ONT64_000586"
     CDS             complement(51243..54860)
                     /gene="rpoB"
                     /locus_tag="ONT64_000586"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR2302024.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            55588..56556
                     /locus_tag="ONT64_000587"
     CDS             55588..56556
                     /locus_tag="ONT64_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2302025.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            56574..57479
                     /locus_tag="ONT64_000588"
     CDS             56574..57479
                     /locus_tag="ONT64_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2302026.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            57476..58282
                     /locus_tag="ONT64_000589"
     CDS             57476..58282
                     /locus_tag="ONT64_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2302027.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            58418..59407
                     /locus_tag="ONT64_000590"
     CDS             58418..59407
                     /locus_tag="ONT64_000590"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCR2302028.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(59724..60248)
                     /locus_tag="ONT64_000591"
     CDS             complement(59724..60248)
                     /locus_tag="ONT64_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HCR2302029.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            60535..62346
                     /gene="scm"
                     /locus_tag="ONT64_000592"
     CDS             60535..62346
                     /gene="scm"
                     /locus_tag="ONT64_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HCR2302030.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(62501..63487)
                     /locus_tag="ONT64_000593"
     CDS             complement(62501..63487)
                     /locus_tag="ONT64_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCR2302031.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(63510..64076)
                     /locus_tag="ONT64_000594"
     CDS             complement(63510..64076)
                     /locus_tag="ONT64_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HCR2302032.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(64090..64506)
                     /locus_tag="ONT64_000595"
     CDS             complement(64090..64506)
                     /locus_tag="ONT64_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCR2302033.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGNYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(64963..66897)
                     /locus_tag="ONT64_000596"
     CDS             complement(64963..66897)
                     /locus_tag="ONT64_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR2302034.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            67178..68134
                     /locus_tag="ONT64_000597"
     CDS             67178..68134
                     /locus_tag="ONT64_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HCR2302035.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            68224..69327
                     /locus_tag="ONT64_000598"
     CDS             68224..69327
                     /locus_tag="ONT64_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCR2302036.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            69340..>69518
                     /locus_tag="ONT64_000599"
     CDS             69340..>69518
                     /locus_tag="ONT64_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR2302037.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
BASE COUNT        21190 a        14021 c        11826 g        22481 t
ORIGIN      
        1 aagcttacgc cgtctttcac tgcaaagact gttgttccac tttcaatact tgttccttcc
       61 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
      121 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      181 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      241 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      301 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      361 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      421 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      481 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      541 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      601 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      661 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      721 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      781 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      841 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      901 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      961 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
     1021 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1081 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1141 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1201 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1261 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1321 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1381 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1441 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1501 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1561 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1621 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1681 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1741 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1801 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1861 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1921 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1981 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     2041 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2101 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2161 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2221 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2281 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2341 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2401 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2461 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2521 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2581 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2641 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2701 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2761 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2821 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2881 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2941 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     3001 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3061 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3121 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3181 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3241 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3301 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3361 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3421 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3481 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3541 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3601 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3661 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3721 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3781 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3841 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3901 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3961 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     4021 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4081 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4141 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4201 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4261 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4321 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4381 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4441 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4501 taatattatc agttagtaac tttttataat cataaaaaaa ataagtagtt ctatctgagc
     4561 gatactcttt tataatactg aattcggaat taaaaaccac gataggtaac tttaaacaaa
     4621 catgaagaga ctttaataag acttctttat ctgaaaacac ttttgtttct ctctttcctt
     4681 tctacatcat taaatagtat tttatacgtt atctaaaata ctattcctct gtcaaaataa
     4741 gctaacttac ttcattataa aatatccact tatcgctttc atgtatattc caccgtaaaa
     4801 agtacatttt atcaccgaga actcatcatt tttcatcatg atgaatacta tttacatata
     4861 tctagttcaa agacaataat tattatgatc cgttattatc taagcactat acagataata
     4921 ggaatttttc gtttctaaat attccaattt gggaatccaa taaaaatttt cgttcaaata
     4981 cgtgtctatt cgaactcttt tctagttgtt aattatgaag ggggaagaaa taaaaaatac
     5041 aatctataca cgaataagaa tattatccaa aaggaggttt tagaaaagat ttattatacg
     5101 taaaaagctc aagacatttt cttagccata aggacatgtt tgatatttaa gaagatatat
     5161 gaattttctt ttctaagtat aaagataact atccattatc aactaaaaaa acatttttga
     5221 aaataaacat cagcagtcct atcaatgcat gtgagcttat agaagttatt cgcagcgcag
     5281 cataattgca ttagcttctt ttcgtgtttt ttgcctcttt tccctttaaa tcttaatgga
     5341 ggaggtgtgc aatatgtcag ataacgacga acgcgtaaat gcccagttga ttcgattctt
     5401 tgaaaaggtc attttgaaca ccgctaatac ttattataaa aaacaagaag aaattcaaaa
     5461 acatgaacaa cttgatcaac tcccaccata tttgtttact aaagatacta taaaatttaa
     5521 acaaccgatt aatgttttaa atgtgactct ccttgttgaa gatataaaat tagcaaaaat
     5581 ccttcctttg ttgaaagaac ctgaacaaat ttttcttatc gaaaaattta tctttgataa
     5641 aactgataaa gaaattggaa aggttctggg gattacaaga cagggtgcga caaatttaaa
     5701 acatcgactt tacaaaaaaa ttcaaaaaat gctggtttaa tatctgcata ataactaatt
     5761 ttgtatgctt ctttgctcgt tctcctcctt cccaaagaaa tagattataa gtccatggga
     5821 agaaggtaga tttatgaatt ctttaaaaga agatttcatt ttagcaaagg ctggaaatga
     5881 agaagctgtt gaggcaattc tcaaaagatt tagctctttg attcataagc aatcctggag
     5941 aactggaaaa tatgatcaag attgttatca agaatgtatg cttgcaatat atctagctat
     6001 ctcgaaattc gaaattaaag aataactcaa ctaagggagt ctgtgacata agtaaaaaga
     6061 tagtttcaaa tttcgaacaa taaatttgaa actatcttct ttataattat aaagtatcga
     6121 ttttacagcg ttctataatg attcatagaa tctaaaacga atgattttaa ttatcaacgt
     6181 tttttaattc gtttttagct taaaaataac ttttgtcaca gtctcactcc taaaattact
     6241 ttatttacgt atgaagtggg caggaggtcg aatccagaac tgggctatgg tacttcaacg
     6301 aggacttctc ctctagaatt gaaaagcatg cgatttacct tccgtaacga cggtagattt
     6361 attcaactat gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag agaattttgg
     6421 ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt gacaaaccct gatactaagt
     6481 tgggctatga tacacttttc ttgactatca gttacgttac cggaaggaat gaactctatt
     6541 gagaaaaaca tatgatcgta aatttaaact aaagatttca caggatatcc ttgagaaaaa
     6601 agtaactacc aagaaaattg ctgaagaata caatatctct cgtccaacaa tttcccgatg
     6661 ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa aaagattacc
     6721 tgataaagca gatttctaca ttcttgaaca ggaaaataaa cgactaaaag aggaagacga
     6781 gtgttctacg aacacaaaga tcgatacgga tctccaggga tcactcaaaa actttacgat
     6841 gaaggaatcg aaatcaataa acgtgttgta gctgttctta tgcgtgaaat gaatttatgc
     6901 acaagaggat ttcatcgacg caaatcttct catggtagag aaaaaacaat caaagaaatg
     6961 gtaaaaaaat gttgaatcga taatttgaac agaaccaaat tgatgctgtt tgggtgatgg
     7021 atattactta cattccatgt aatgacggtc gcctctattt gtccacctat attgacttag
     7081 caactcgtat tctacgttac tatatggtag attcacacat gaaaaaagag attgtcgtcc
     7141 gtccattaca aatttattaa ggagaattgc ctacggtaat tcattccgat agaggtagcc
     7201 agcatcggtc ttttatttat caagagctgt tgaacgacca ccacatcaca cattcagtga
     7261 gccaaactgg gactcctgtt gacaatgcag taattgaagc gtttcatcga tcaatcaaat
     7321 gagaattaat cgactccaac aaatataaaa gtaaaataga gatgaaggtg ttaatccaag
     7381 attatttaga agactactac ccaaacaaaa gaatccatac aaaatttatg atgactcctt
     7441 gacaatttga agcgaagaaa ataagggatc tataatatct agtgttggtg acgccttctg
     7501 ttgccaatac tttttctttt ttctaataaa cgcaaacaga caccttaaaa tcctttagaa
     7561 taaagtcgac gaaaaccatt caaaggagtg tttcaagatg tcctattcag agtttaccaa
     7621 agagttacta gatattctag acctaaatct tatctttcat gaagatgcct ttcgcaaaga
     7681 gcgaatcaat gacgaaacat gttttgtttt tgatggcaca ttgacctatc aaccggagga
     7741 atgctttcat cgccactatc aaaatgaaca aacgatcatt aaatagggct ggaaaaaagt
     7801 atccatctta ttgaatgatg tcagtaacta caagaccatt ctgcggatta acaaacagcg
     7861 cttcaaatgt aaacactgtg gaaagacttt tttagcagaa gattcagtta gtgatcgtcg
     7921 ctgctccatt gctcgcagag tcaaacaagc cattcttgag ctattaagcg aacctctttc
     7981 tatgtctttg atcgcacgta tgaagcacat ttctccgacc actgtcattc gaatacttcg
     8041 cagccttcgg cctaaaacca tttctttgag ccagctgtta ccagaagtgg tgtgtttcga
     8101 tgaattcaaa tcagtgaaaa atgtctcggg agccatgagc tttgtcatga tggacggcaa
     8161 gacccatcaa ctgattgata ttgtggaaaa tcggcaactc aatcctttac gcgactattt
     8221 cttgcgctac cctataaaag tccgtgaaca agtgcgtcta gtggtttcag atttctatac
     8281 accctatcgc acattggtga aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt
     8341 tcatattagc caacacattg gtcgggcttt taccaaccac cgaattcaag taatgaagac
     8401 ctttaaaaaa ggggatcgtc ggtcgaagca cttaaagaaa tactggaaac tacttcaaaa
     8461 gaatgcgtgg gaattgaaag gtcagcatcg ctactggcgt ccgtcatttc gagatcatct
     8521 gacagaagca gagatagtcg atcggctatt atcttatgat gattccttaa agcgaggcta
     8581 cgaagtctac caggattttc tttccgttat cagaagacaa gatgttcctg aattcgtcgc
     8641 gctcctaaaa gaagactaca aggagttgcc agagcactac caaccagtgt tcacgacctt
     8701 caagaaatac caaacagaaa tcaaacgagc gttgcgcgtt ccctattcca acggccctat
     8761 agaatgtttg aataaccata tcaaagtact aaaacgaatt gcctatggct atagaaactt
     8821 tcaaaactac cgagaacgaa tctttttata tcgaggaaaa tattttaaga aaactaagaa
     8881 tactacccaa ctgactaaag ccagaacaac aacacggcta gacaagatag ctgtctagcc
     8941 gtgtgagtat ctcgattatt ctattgaatt tcttcaccaa cactcattga cgaagagcca
     9001 aaataagtaa tctaaccgac tgttaactaa accattgcag gtcccgttaa agatacttgt
     9061 gcctgttgtc accgcttctt tttcttgtcc acaagcgctt aacacactta ataaagcccc
     9121 tgctattatt aataataata tctttttctt attaaaaacc tcctcatcta tttccagatt
     9181 agtaaatgta gtaatctgtg ctttcttttt tctcaatact aataactaac ttatggggta
     9241 aacaaatact cgtttgaccg ggttttgaga tccatccagt tctaaccgcg atttgatcag
     9301 gactattatc ttctttaact cgaattcttc catctttaat ctcaacaata ttatattgtc
     9361 ccttcgcagg atgataattc acgagtttat gattgatctt atccaaatcg aagcgatcca
     9421 tttccttgcc atcaattcga acgatggcat atttgatagc atcttcagtt gtttcattct
     9481 ccttttctat agagaataca aataagggaa taaaagaggt aataatcaaa atgccaataa
     9541 ttatgacatc gcctatcttg acaccagctt ttcttaaaaa tttcattaac gatatctggc
     9601 taaacatatt agactttttc catggaaatg gtgaaatttt tcggaaatta tctgcattaa
     9661 gagaatcagt taaacaaccc tcaagttgtt ccttaataca ttgttgatcc atcttcatac
     9721 caataaaaac tagtttatta atgcgttcac caaacaacgg atgccaggct tccttcaatt
     9781 cgggattttt ttgtataatc atttgacact caattttagg taaactagct acccaataat
     9841 ttaatgattc aatactttta tagctacccg atgtttcaaa gatcgtcgaa aaatcactat
     9901 gttgggcaaa ccaaagaaac ccttttgctc ttgtaatatt gccaggaaaa tttttatccg
     9961 taagatgggc aaatttttgt gcgtcaagga gtaaccgatt tcgatagata aaacttgaga
    10021 taccatattc ttctgtttca ggaatatgat tttgatagcc aagctctaac tctttgaacc
    10081 agcccactga ataggaagtc tcatctatat taaaaagatt tgtatttaga attttcgata
    10141 agtctacttc acctttaatt gtcctaatta ttttggcatt ggactgtaac ttagcaatta
    10201 gaccgttaat tttgtaaatc gtcttggttg acactaagtc actattattt aaaagtaata
    10261 tgttacaaca ctcgaattga tcaattaaca agccacgaat atcctgttct tcttcaacta
    10321 cattaccttc agcttttctt tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac
    10381 gttttacatc cactactata ataatggcac caatctctaa tcgttgggtc aaatcaatgt
    10441 ctaaagggtt tttagctaat ataattgctt gaataattgg aattggtttg ctgattcctg
    10501 aagcttcaat aacaatttga ttcaagtcag aaaattgcac taattgatct agttcaacaa
    10561 tcaaatcttc gcgtaatgta caacaaatgc atccattcgg catcgaaaca agtttttctc
    10621 cagttctttt aaaacctttc ttgtcgacta ataccccatc aacatttact tcccctatgt
    10681 catttacaat gacagcaatt tcctttccgt gatcaccgta cagaatatga tttagaagta
    10741 tcgtttttcc tgaaccaaga tagccagtta agattgtaat aggtattttt ttcataggga
    10801 acctctttct ataccttgaa tttacacttt aagtgcaaca cataaaaatg agtcataatc
    10861 gatatccaac tagtataagt ttgtcgagaa cactcctaga aggaaaataa ttatgagcca
    10921 gtatcgtcat cttactatac tcgaacgaga aagaatcttt ctgcttcacg aagaaggatt
    10981 ttctattagt ataattggca acgacattca gcatagcccg tttactattt tacatgaact
    11041 gagaagaaac aagaaaaaaa ctattcacct tccaaagcgg aaaaaaatta tgcttatcga
    11101 agaaaaaaat gcggacgtta tcacattcta aatgatccgt gagtttggtc aattgttcgt
    11161 catttatttg tcgattttca gtggtcgcca gaggaaatct cgaatcgttt gaagcatgaa
    11221 catatggact tttcaattag ttttatcacg atctaacgtg caatttgcaa tgatcttttc
    11281 gatacagaac ccctctcaca cggcaataaa ggcctgatcc gttcattgca ccatcgagga
    11341 aaaacaagac atacaaaaaa tcatgtcgag tctcgtggaa agatttgcct tactcattcg
    11401 atccatggcc gtccaaaatc cgcagatgat agaagtgatt ttggtcattg gaaagccgat
    11461 attgtagctg gcaaaactgg tggtacttgt ttagtgactt tgacagacag aaaatcatgc
    11521 ttccttttag caggaaagaa ggtccccaga aagaaagccg cttatgtgag cgagaaaatg
    11581 attgaacttt taagcagtat tccaaatgag gcggtcaaaa cgatcactcc agatagagga
    11641 aaagaattct ccaatcattt cgcagttaca gaagcattga ataatatgcc cttttatttt
    11701 ccagatcctc atgcccttgg caacaatgaa caaattaaaa tacgaataga ctcattcgag
    11761 aatacttgcc aaaatcgaaa gagatagcta cggtagataa ctcaacgata aatcagatga
    11821 ttgaaaaact gaataacaga cctagaaagt gtttagattg gagaacacct tatgaagtct
    11881 tctttgataa agtgttgcgc ttgatttgac aattcaagac ataataaaaa gcagatcaaa
    11941 tccaaataag ggaaaaatga tctactctgg tattatcaat taattggcca agctattatg
    12001 ccgttttgct aaacgtctac ttcttccagt attgaccttg ataaaacaac aatcaggatc
    12061 ccgttttaat actctatgaa ctgtgtcaat actaacatgt aaagttgcag caatatcagc
    12121 taatgtactc ccatttaatc tcatttgctt tgcaaatcga taacgccgat aagcatcaat
    12181 attcttggaa tccatagaca ataatacttt tcttaccatg tatttcctcc aaaaattgaa
    12241 ttttaatgag ccatcatttc ttgagcaatt tcatgtttag acaatgaagc tggatagaaa
    12301 gtaggccaat tatgcaattc attatagagt ttttcttgac tttctccacc aaagaaaata
    12361 tggaaatgct ctgccttact tggtgcaatt ccatgatcac taaattgaat atatttaaaa
    12421 cgaccagcac ctgcatcatc agtttcaaaa ttaaaacgaa caccacggtt tcctttttca
    12481 taattcaaaa ttttgtatcc aacatatttg tatgtgtatt gatgatgttc accgttgact
    12541 acaaaatcaa tggtgttgtc tttaatgttg atagtatcaa tatctgtctt atagccagta
    12601 gtataataat ctttgtactc ggcagctgtc atgtctttat tgatttttgc tttgtaatcg
    12661 aaaacttcat ccaaaattcc atcttttaac aacggataaa cagattgcca ttcgcctgta
    12721 taatcactca attcgcgatc cttcacatcg gcatctttaa agtacccatt agcaactgtc
    12781 ttttcatcct tcgcttcttt ttctggaact acctcttcac cagcaaccat agttgttttt
    12841 tctaacgctt ttagattgtc ttccattact gaaacataat cttcaccatt atccatttgt
    12901 tcttttgtaa gactttcaag aggattcagt acttccaatt ttatcccagc ttcgttggcc
    12961 aaagtttttg caattttatc attagcattc ttttcaaagt aaatataatt gatcttattt
    13021 tttttaacat actcttttaa ttcagctaat cgaccagaag atggctcttc ttcaggactt
    13081 agtccagcaa taggaacttg tttcaatcca taatccaaag ctaaatatcc aaaggctgcg
    13141 tgttgcgtca caaaactttt ttgtttcgct tctttaagac tagtggtata gtcagcatcc
    13201 aaccgtttca gtttagtcaa atacttttca gcatttgtct caaatacttt tgcttttttc
    13261 ggatacaatt taactaactg atccttgata tttgaaactt ccttaattgc cattttgggt
    13321 gaaacccacg tatgaggatc tagttcatga tggtgatctt cttctccgtg atcatgatct
    13381 tcttcttcac tacctggcaa taaaatcatg tcttttgttc cttctactac attaggccca
    13441 gctttcttcc aactttcttt agcttttggt acccatgatt ccatgttttc gttatgatat
    13501 acaaaaacat cagcatcatg agcttttgcc atatccttag ctgagggctc atagtcatga
    13561 ggttctgatc ccgctggaac cattaaatcc acgttcgctt catcgccgac gatgtttttg
    13621 gtgaaatcat acattggata gaatgttgtt acaatttcta attttccatc tttatcattc
    13681 gatacttttg catcattagt gctggagcaa gctcccaata atcctagagc tgctatccca
    13741 ataagtccta cactgatttt cttcattctt tttccttcct taattatgct aatttcttca
    13801 ttttatttcg tttatgttct ttgataattt ttaatgccct ttttctagtt ttaatattcg
    13861 ggcttttaag tcgtcgatat gcactagata tatccgtttt ctccattaga aattaccccc
    13921 aatcactact tatcattatt tgctttccgc cagtaattta tcaaaactct gtttgatcct
    13981 ttcttcatcc aacatttttc cgattagaat aatagtggtt tggtcttttt gtcgagtggt
    14041 gtattgaaaa ttaacctgtt cgtttacctc ttgcatggca actataaaat cctgctcttg
    14101 taaagcaatc aatcctttaa aacgatacag gctcccttgg ttggttaaaa tcagccagtc
    14161 aatccattgg agtaaaaaat ctttttttat gtcttgatct gaagttaaga gaattgattt
    14221 aaactcatgt acttgatggt gttgatgata gtatttgcca ttatagtgat tttctgatga
    14281 atcactttca aaaactaagg gatgattaaa ttttttcaaa ttataaaaat cctttttttg
    14341 aatcgtttca ctatgtgtga acattcgaat atcggataaa gggttaatac ttttgatttc
    14401 tctctgtaat ttttttagtg cagataaatt caagttttgt tttctagaca caaaaatgcg
    14461 atctgccatg acaagttgct taagggcttg tggttctctt tcaataagtt caaaactgtc
    14521 actatcaata acagtcaata aactatcgat atatactctt cctcttagtt gaggcgctgt
    14581 taaaagtgtt tgaatgattg gttggggatc tgctatacct gtagtttcaa tgatgatttg
    14641 tgaaattggt tgttgatgtt cttctattgt ttccagtaaa gaaatcaata ttgctaataa
    14701 atcactgcga agactgcaac acatacatcc attatttagt tgtaacaccc gttcctcgct
    14761 atgaactaat aatttgtgat caatcccaac ttcaccaaac tcattttcaa ttataataat
    14821 ttcttccctg gggtagggtg agacttgcaa ggcttgattg attagcgttg tttttccagc
    14881 ccctaaaaac ccagaaacga ttgtaactgg tatgcccatt ttgtcacctt tttaccactt
    14941 agctatttca cttctttaaa aattgcccgt ctgcgcaatt ttggcgaata cttttttaat
    15001 tctaaccgtt cagggttatt ccgtttattt ttgctggtga gatacagacg ttctccagtt
    15061 tcgacacatt ccaaaataat attttgacgc ataaatcttc ctccttcaat acttatcgat
    15121 cttcaagata tgcttgaatt tgttgtgcat cttcaaaagt taactcgcca gattccaatt
    15181 gtgaaactaa attttctttc atatcaacag gtatctccca ataaccatcg atacattgaa
    15241 ataactcctc gtcatactct tcaaaaattg gatagattct taaaaaatca tcaaatgata
    15301 agccttggtc ctcatagtaa tctttgacta atccattttc aattgcctgt tgggtcggta
    15361 aacccgtttc agtaataatt cctctctcta ccatcgaacg ataaaaagtg catttatcta
    15421 gcgattgctg ggtcgtttca agataatact ttaatccttc ttcaactaaa ggtgggtaaa
    15481 taatttccat tttgattccc tcctaaaagt tatatattaa actattttga agctccttta
    15541 attactttct ttccattata gtaccctttt aaagatacat gatggctacg tctataatca
    15601 ccattcgctt cgtcaaatga aatttctggt tttactacag tttgatgagt tcttcttaac
    15661 cgtttcttcg ccttagatgt ttttcttgct ggtactgcca tgatatttcc tccttttacc
    15721 aacttgcttt ttttactcca ggaatcaacc cttgatgggc aagttctcta aacttaatac
    15781 gggacatacc gaactttcgc atataccctc ttggacgacc atccgtctga tcacgaagcc
    15841 tcagacgatt cggatttgaa tctcttggca atttagcgag agcggtgcga tctcctgcgg
    15901 cttttaattc ctgacgttta tctgcatact tttcaatcat cgccatttgt ttctttgctt
    15961 tagcaatttt cgactttttt gccatatacc tttatactcc ttttattttg atttagacac
    16021 ctcaaatcgt aatcgttacg ttttgacact gatactttac caacaagagt tgctcttgtc
    16081 aacaataaat aacatgaaat aaaactcata tgaatagata actatttgaa aacgaattta
    16141 tcggtacata gagataatca aatcatttaa aagaatatgc agtttaggct ctgaaaaata
    16201 agaattcact tttattagtc caatcaaaca caaataccgt tttaagtagc ttttcctcta
    16261 cttaaaacgg caatatgaaa gaaactataa ctttttattc aatcttttat gtttaactgg
    16321 tttatcgcca attttttaaa actttgatta ttccttatat tataagaata gacattaagt
    16381 gaactaattc aatcatactg attctttcgt gtattcaaaa ttagatgctg tttaataaaa
    16441 ttattctttt tgaaaaagaa ttataactat atcccaaaaa ttgtaggtta atttaaagcc
    16501 tatttcatca tcgtgattga gcttcttttt catagacgaa tttctcagat agtttagttt
    16561 tctttatgag atttttgtat atttctgacc ttcgatacaa ctcattatga gtaccttgat
    16621 caactagttt tccttgatcc atcactacaa ttttatttac cttttcaata gacttcaagc
    16681 gatgcgatat aattagtact gtcttatctt gtattaaacg atttaaactt tcctgaatac
    16741 gtttttcatt ttccgcatct aaactggcag taatttcatc taaaattaaa attggcgcat
    16801 ttttcaaaaa agcccgtgct atggataggc gttgtcgctc tcctccagaa agtgaacttc
    16861 cgttttctcc gaccaatgta tgataaccgt caggtagttt ttcaataaag tcttcgcagt
    16921 tagctaaacg agctgctttt ttcacttcct catctgacgc ttgactattt ccaatacgta
    16981 tattttccaa aatacttgta ttaaacaaag ttacctcttg aaaaacaata gcgattttgc
    17041 taaacaaaga agctggagat acacgtttaa tatcataccc atcaattaaa atacagcctt
    17101 catcataatc atacagtcta gacactaatt tcaagatact agttttcccg gaaccactag
    17161 cacctactaa cgcagtcact tctccttgtt tagccgtaaa agagatatga tctaaaattg
    17221 gggtattgtt atcatatgag aaagagacat ctcgcaactc aatgtcaaaa gatttcagag
    17281 gactatctga tccctcttgg atactggttt ccttcatagc tctaattcgc tgtattttag
    17341 gagctaaata aaatatttca agcactgctt ctttcataga atcgaaggaa tcctttattt
    17401 ttattgcggc tagtaagtaa ccaacgacgt agagtatagt cacttcaccc gttaacagta
    17461 agtgaacacc tactagaatg actactgcta aactaacata tgagaaaata gaggataagg
    17521 ccatgactga aaaagtactt aattctacct ttaaatgaat cctttcactc tcttccattt
    17581 ttttgaaaag tctgtcttga acttttttag atagattaaa gctattaatc tcttgctgca
    17641 attctatagt ttcttgaaat tcttccgagt tttctctcaa agtatcgtaa tatttagtat
    17701 tggctttcgt ttgggatttc tttgataaca agattaagac aaaactaaat aacgttggca
    17761 aaataacagc taatcccatt ttgacattac caaccaaaag catcactgaa ataaaaggga
    17821 aaaacagagc cataccgccg gctttaggta ttgcatgact catcgcatgc tcaatacctt
    17881 caacgtcaga catgatagtt tgagataaat ctgataaatt atgttttgaa aaataggata
    17941 atggtaaatt tgacaaatcg tctgcaattt gcactcttaa atgcgcactt tctttataag
    18001 tactattata taagttttca tactccctat ctaacaacca atacatgaaa agcaaagtaa
    18061 caaacgatac ggcaaaataa agccaacggg gctttgtatt ttctaaaact aattcgtcta
    18121 tcagcatcat tataatgaat gctggtacca tattaatgca gtatacaaaa aaactagaaa
    18181 tagaagcttt agttaaagct tctgcgccct gatcacttaa agcaaatttg tttttataaa
    18241 aacttttcat tgtcaagcct ccaatcattt gcattctcat aattagtgac caattgttta
    18301 tacacttgat gtttagccat taattcatta tgatttccac gttctactat tttcccttct
    18361 gacaagacaa gaatttcatc tacagattta atgctggata atcgatgagc tatcataata
    18421 actgtttttt ctttcatcag atgttgaaaa gccttttgta gttcatactc attatctgga
    18481 tcaatagatg cactggcttc atccataata acaatctttg catcttttaa aattgctctg
    18541 gcaatagcta tacgttgttt ttctcctcct gacagataaa ctcctttact accaattaag
    18601 gtatgctctc tttcgggaaa tttttctaaa atcgattcgc aaccggctaa tcttagcgcg
    18661 tcaaatactt tacttgctgg cgcttgagga tttgctatag aaacgttatc gtaaatactc
    18721 tgattgaata gtttcgtatc ttgaaagaca aaagcaattg tgttaataat tgtgtctttt
    18781 gaatagtgat ttaaaggctt accaccaatt ttaatctgtc ccttatccac attgtagaaa
    18841 cctgaaatta gtttagctat cgtactcttt ccgcttcctg attttcctac taatgcataa
    18901 attctttttt gttctaaaac taagttgaaa ttttctaaaa cacactcttc tttgtatgaa
    18961 aagctgacat tttcaaatgc aatgtcacaa tttttaatct gtgtctcgtt cccatatttt
    19021 agttggtctt gcttcatttc taaataaagg gagtctagtg tttcgactgc atatcttgct
    19081 tcaaacaagt gcatacttac atacattatt ctcatgaaag aaacaaaaat aattccactt
    19141 aaaaaaaaca acatcaacaa gtcaacagct aatatcttgg gagtggtaag agaagttata
    19201 aaaaaggtta taggaactat caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga
    19261 acaaaaggtg ttttcccact caaagaatac ttatatgcaa acttagcata atcgttaatt
    19321 gctttattca aggctttaaa tgatgttact ttagcaccaa atatctttat aacttgaatt
    19381 ccgcggatat attctaccgt ttcagagctt aacacatcta acgattcttg atatttttga
    19441 ataaaatttg tattctctgt cattttcttt aacaagaata caccaattac taacatggcg
    19501 attagaacca atccaatttt aaagttaatg aaaaagccta aggcaactga taaaacaggt
    19561 gttactatgg cttgagcact atctggaatc aagtgagcaa ttgccatatg tgttttagcc
    19621 gcgttgtcat caattgtctt tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa
    19681 cttgcttctg ttaaaccgct aattcccttt tttctcaaat tagtctctaa gcgaaaccca
    19741 aggatatgag aaaataagcc agaaaaaata taaaataaag ctcctaaagt taaccaagca
    19801 acagtttgaa ccgcataact cttagctaaa tattcatctc ctacaataat tagagactga
    19861 agaaatttgt aaatactgta atatccatag actgttaata cgacagaaaa atcagaaaat
    19921 aaagtggcta accatccgta gtatttcatt ttagggacat aactaaataa ttttttgtat
    19981 actttcattt tttcctcccc cgcattcaat ttcttctctt attcgtttac aactgttaat
    20041 gctttccgta atagcgtaaa ttagagatgt acaaacagat aaaaggtaaa ctacgttcta
    20101 ttttttgtat ttttttaaga cttttcttcc aatcaataca ccaatcatag ataaacttgc
    20161 tacaactaac actgaaatga ttccccatat acctaccgca tagtggatca ttgcatccat
    20221 tgttgcagca gattgtcctt tatctatcca atcttgacga aaacgatctg taaagaacca
    20281 tataggaatc cactgtccgg cagcactgcc taattgcatt agagcccaag acgcaacttg
    20341 aggaattgct cgatcatatc caaatacaga tagaataata tcagctaaga tcgccatact
    20401 aatcgaactg ataaaccaag gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa
    20461 aacaattgtg taaatagtaa aaattccttt tctagggtag ttatagctaa tataaacaaa
    20521 tatgattcca gataataatc caaaaatccc gggggatatg ctgtgaccaa acgccccacc
    20581 aatactacta accattattg aaaaaaagta aattactagg gaaataactg tagctaaacc
    20641 aataaaaata taatcttttg ttttcatata tattctcctc aattctattt cgttatgtca
    20701 ttaaaaatct gttctatcaa agttgaatct tctactctaa gttttatatc tttttttatt
    20761 ccaccatttt ttaaataaat aattctattg catgttttat aaagaaattc cggatcatga
    20821 gtagctataa ctactgcacc cttttctttt aagtctttaa taaggtcagc aatcctttcc
    20881 atattaatat aatccaaccc acttgtaggt tcatcaaata gaaataaatt agtttttgac
    20941 aatgaagcta tagaaaccaa taatctttgt ttttctccac cggataaatc aaacggtaat
    21001 tgatctttca ttttataaag ttccattttt tttaaaattt tttcttgctg atctttacta
    21061 actatttcat tccccagggc taactcgctc ctcactgatt ctgtgaaaaa ttggtaatcc
    21121 atttcttgca ttactaaaaa cggcgatgac aaattttttt tattttcgat tatctttcct
    21181 ttggtagggc gaataatctt catgagtatt cttagtaagg ttgttttccc cacaccgttt
    21241 ttccctacca aaccaataac atctccaact ttgacatcaa aatttatgtc ttttagaata
    21301 ttttcataag taatttcttc aacttttaat acatctgcac ttaccttttc gatataggga
    21361 acttcaatcg aactattaaa aatgtcaaat ttccttaatg cgttattgtt ttcagttttt
    21421 aacaactgaa tagttgtagt ttttacagtt ctatttttaa caaatagaac ttgatcaatt
    21481 aattctttca aatagtaaaa acgatgttca gctataacaa tagtaaatcc tttatttttt
    21541 aaatcacaaa gtaagtcagc aagtttctta gtcgctttga agtccaaatt tgaagacggt
    21601 tcatccaata taattaattt agagtttagc gataaactac atccaataga aattttctgc
    21661 cgttcaccac ttgatagcga aaaaatgttt ttatctaata tatttgaaag atctaataat
    21721 tcagctaatt cattgattct tttatccata tgctcttttg taaatccaaa gttttccatc
    21781 gaaaatacta actcactcgt tgtatttgtt gtaaagaatt gtcctctagg atcttgactt
    21841 acgtagccaa tattttttga tatactttct tgtgttaagg attgtagcga gttattttca
    21901 aataaaattt ccccaaccaa ttttccgtca taaagtcttg gaataagtcc attcattaac
    21961 tttaatagtg tggatttccc actcccagaa gaaccagtaa tcaatgtaag actgcctttt
    22021 tttattgaaa aattaaggtc aacaagcgct tgttccttaa catccttata tgaaaaagaa
    22081 acattgttaa attcaaacaa ttctttcacc tcaaatcaaa atacttaaga aaaaaatagt
    22141 aacggaaaac aggatgatat agtctaataa gctcatgttt gttttatagt aagaacttct
    22201 tttcacagga gattctatcc cctttgttat tgcattagcg gtcatttctt cagcaatcat
    22261 agttgcacgt ataagaacgg gaactaacag aaattccaaa tattgcagag gctgaaaaaa
    22321 agacaagcga atgtttctca tttttaaaga ttctttaatc attcttattt cagaacgata
    22381 agtcggaaag aatctgatca ctactgtaat acttaacatt atttttttcg gtaaatgtat
    22441 tttttctaaa gaggataata attcattgct agtatagaca ctaaccaaca accatgagat
    22501 cattacgacc ggcaacattc gaaatacaat atataaggat gtcgcaaaaa atatagtaaa
    22561 aaactctatc ttgatagcat caataatttt cattactaaa aacagaactg aataaatcaa
    22621 gatgaaataa atacatgtct ttattttacg aaacatcaat actaaaactg tagcaataat
    22681 gaaacacccc acttgaaata tatttccctt tcctaaaaaa ataatgaccg gaaaaaaaat
    22741 atttaaagta atcaaggtta acggattgat ttttgacact ctttcacctc caaaaaataa
    22801 gatcgcactg ttatcttaag ctgataaaaa tgcaccgtta ttcagataac agtgcgatct
    22861 tatcaaaaag cataactgag aacaattctc aatgcaagct ttttttaatt aactcagtaa
    22921 ttcaatccac ccaggaacag aaaattgata caatcttctg gttaatagca tagtcttttc
    22981 ttttgaatag ctatgcttca atatatcata gaatgtagcg tagaatgagt aaatgtagaa
    23041 gtggatttct tcttcattaa agactaaatt ttgattgatc attccgatag tctttaactt
    23101 atctagacaa cagactgtct ttttcgtcac aaatgatgta attttttcaa caatatcttg
    23161 atatttggtt ccagctgatt ggaataaaat caaatcgaat atacgtttat tctcaaaaat
    23221 ataattaata aatggatcta attcattgaa gttctctctc caacttttct ctatatctat
    23281 attttctaag tttaagaggt atttggtaaa ctcttcatta tacatattaa taagcgcatt
    23341 aatatgcggc tgaataattt cttcaaataa cgcttcctta gaagtaaaat gtttataaaa
    23401 agcacccgta gttacgcctg cggtcaggca cagttgcctt atcttagttt gctgaaatcc
    23461 ttctttttca aaatggctta gtccactttc tattaacttt tggtgagtta taatataatc
    23521 tctggtttct atgggtactt cctcctaata agatgaatta actttattat cttacacagg
    23581 acttgaagtt tcaatcgtta acgtttatta ctgaactcat tccttagagc taaactaatg
    23641 agaaagagca gaataactga tacaaaaagc aatcctacaa gaattaatag actcatctaa
    23701 atcattcacc ttttctaaaa taatccgaga aagatttaaa aaaattatat ccagctatta
    23761 ggataagtcc tatcataatc actccaatag ccattttctt atcctcccta aaaataaagt
    23821 cttttactta aaaccttttt tgtaatcgtc aataaatttt ttccctttat cagttaattg
    23881 aaatcctata tctaaatagt cctcatatct aagtttagcc agcatctcac ttacagatgc
    23941 tggactaaca cttagaactt ttgctaaatc attatttttt aatttctttt ttctttttga
    24001 taattcctga attgctagta aataaattag tatagtagtc gttttaattt gtaccatacc
    24061 taccctccaa attatttcga aaggccttca gcaattttat ccaggttcca tttcatcatc
    24121 gcatagtaac tatcaccatc ttgtcctttt tctgcaattg aatcagtaaa aatcgttgaa
    24181 tagataggaa tattggtatc ttttgatact gttttcattg gtctatcatc cacactgctt
    24241 tctacgaata aggagggaac ttttgttgtg cgtaattttt caactaagtg ttttatttga
    24301 tctggtgttc cttcttcttc agtgttgatt tcccaaatgt aagcagaagg cacattatac
    24361 gctttcgaga aatatttaaa gcatccttca cttgtaacaa tcattttttt atcattcgga
    24421 attgaagcaa atttagattt agcttctttg tctagagaat ccaatttttc aatatactta
    24481 tctagatttt ctttatagaa ttttttatta tcaggatctt tttccgctaa ctgtttttca
    24541 atatttttag cgtaaataat accgttttct aaatttaacc aagcatgagg atcttccttc
    24601 cctttctcac tctgaccctc taagtaaata acatctatgc catcacttgc tgcaaaatag
    24661 tctttgtttt cctctttgtt cgcattttta actaattttg taaaccaagc atttcctcca
    24721 gtttccaagt taacaccgtt ataaaaaatc aaatctgctt ttgaagtttt ttgaacatct
    24781 tcaggcaaag gttcatattc gtggggatct ttcccaatag gtacgatact gtgtaaatca
    24841 attttatctt ttgctatatt ttcggtaata tctgctaaaa tcgaattagt aactactact
    24901 tttaattttt cgttcgaagc cactttatct ttgtctttac tatttattga acatgccccg
    24961 aatataaaaa gagcaatcaa aatccccaga cttcccagta ctgctttcca ttttctcatc
    25021 ataacgatct ctccttcttt cttaaaaatt tttgttttgg tgagattaga aaactaatta
    25081 caaataagaa tgccgctgtt aaaacaatac ttgatcctgc tgctatgtta aaactataac
    25141 caataaacaa tccaagcaat gatgaacttg ctccgactgt tgcagaaata aatatcatag
    25201 ttttcaagct atttgcatat aaatatgctg tagctgctgg tgtaattaac attgcaacaa
    25261 ttaatatagt tcccacactt tgcatagctg ttactgctac taaagttaat agaaccatca
    25321 atagataatg ataaaaactg acattcatcc ctattgcttt tgccattaat ggatcaaaag
    25381 aagtgattaa taattcttta aaaaatagaa ctactataac caacactaaa accgaaacgc
    25441 caattgtaat ccacttatca atatcttgta cagctaaaat atttccaaat aaaatatgaa
    25501 ataaatccgt tgagctattt gcaactccta taaggataac tcctagtgct aaaaaagaac
    25561 tgaaagtaat accaattgct gtatctcctt ttattacact attgcttttt ataaaagtaa
    25621 taatgataga agctaataga ccaaatacaa tcgcaccaat aaaaaaatta atacctaaaa
    25681 tataagaaat tgctactcct ggcaaaacag catgagaaat agcatcaccc ataagagaca
    25741 ttcctcttaa aataataaaa caacctactg ttccagaaac aatccctata actacagatg
    25801 taattagagc attctgtaaa aagtgaaagt catacagacc attaataaaa ttttgaagca
    25861 tattattccc cctctccaat aaatatagta tctccatagg ctttttttaa attttcttca
    25921 ttaaatacag attctacact tccgtgagca attagatttc gattcaaaag aataatatta
    25981 tcaaaatact tctttacctt gcttaaatca tgatgcacaa ttagcactgt ttttccctga
    26041 tgtttcaatt tttttaacgt atccataatt attcgttcac taactaagtc tatccctaca
    26101 aatggttcat ctaaaaatat gaaatccgca ttttgagcaa gacatcttgc taaaagtaca
    26161 cgttgaaatt gtcctccaga taattctcct atttgatgat tagaatattt tcccatatcg
    26221 accttttcca aagcctttga aactctttgc cattctgcat ttttaattct ttgaaaaact
    26281 ttcattccgg catatgttcc catagataca cattctttta cttttattgg aaaagtaaaa
    26341 tcaatatcag ctttttgctc tacatatgca atcttcacca gttttttatt gatatcttca
    26401 ttattaatgg agacctctcc ctgatgaggg ataatattta acattgcttt aagcaatgtg
    26461 gattttccag caccatttgg accaataata cctgttaaag caccttcttg tatttttaaa
    26521 gaaatctcgt tcaatgctaa aaaatcgtta taacaaacat taagcttttt cacttctaac
    26581 ataactttct ccttctaaat gataatgatt atcacgatca tatggtatac aaaaattata
    26641 ttttagtcaa ccctaaattt tatagtttag atttttttct gttgctgaag attagaatga
    26701 caaaactatt tgaaactacg cgtatagtaa gtagctttca aaatatataa aaaaggagcg
    26761 ttctcaaatg agaacatgct cctaaaacta ctttattggc gtatggaaaa attatacttg
    26821 aagtggatag cgggttgaat aagttgataa gcatcaaaac atttataaaa attattcaag
    26881 actatgtctg ttccatatac aaatatgaaa atcagtaaac tccatttaac ttttttcgta
    26941 gtgtgtcttt ttatacatgt ttatcgttat taatagtgaa taggaaaaaa actatttttg
    27001 atcattgaca attaaataca atttttgaat aacgatccct gctattcgag ctagaaactc
    27061 aagtacgcca tgacctgatt agcgacaaat acttagagat ttctttttgt tctgggaaaa
    27121 caaaatgcca tgacaatagc agcaaataat gatacttccg taaggctcgc cgtaaactgg
    27181 ggagaggtga aattcctatg tgcttaccca aagcaattca aaaatttcat aactactatt
    27241 ttaggagatg attttataga atccagagtc tacctgaata aaaaacaaac ctgtgcattt
    27301 ctaggaatcg ataataatac tctagataag tggattcgta tatataattt cccagtaata
    27361 agagttggtg gtgtagttcg ctttgacgta gaagatatta aagactttat gaatacattt
    27421 aaaataactt gaaattttct tctcgccgca tatactaaaa ttagtgaaat atactgcctt
    27481 acttttttta aggagtgagg attttgccta caaagctttc aaatgggaaa tataaaacta
    27541 atctgagata tccaaaaagg tttaaagaaa taacaggaat tgcatcagaa aagtatcaaa
    27601 aaacatttcc aaataggcaa ttggcgatta aagccgaaaa tgatatgaag aagaaaatag
    27661 aaaaagtttt gagagaggag aatgcaaact cattagaatt aaaaggaaaa ataacgttta
    27721 aaaaatttta cgaatctaaa tggcttccta gatatgaatt aggtcaaaca attagatcaa
    27781 acaggcctcc tagtgatata accattagta atacaaaaga tatatttcgg ttgcatattt
    27841 tacctatgtt tggtgaatac gcaatgaatt atctaaatat caatactgag ataatatcag
    27901 atgaattaac caaaaaatcg aaagaatatg caaatataaa aatcataaaa ggatatgttc
    27961 gttccatgtt cgatattgca gaaatactaa attatatcga atttaatcga actactaaaa
    28021 ttattcaaag tatcactgca cctaaaaaaa atgctttaga agaaaaaaga atacaagaag
    28081 gtaagcaagc actatcatca aaagaactta ctaattggat agaagcagta aatgatgatt
    28141 ttaataatca tttacttact tttcatgatt acacactttt tatgattacc ctgtatcttg
    28201 gtgatagaaa aagtgagact tacgctttgc aatggaaata tattgatttt gaaaaacaaa
    28261 cggtccgtct taaacatact ttagataaat atcaaagaaa aaagtttaca aaaggtcgaa
    28321 aagataccgt cattcaagta ccagaagttg taatgacatt gctaagtgaa tggaaatcgg
    28381 tacaagctga tcaattgttg aaattaaaaa ttaagcaaac gttagatcag tatctcttta
    28441 cttatactaa accatctgga gaagtaaatt gtcctgttca tgccgattac ctaaactatc
    28501 gaatcaattc aattaaaaga aggcatcctg acttagttca tttatctcca cacaaactcc
    28561 gtcatactta tgcaactata gctagacaag gtggagcaga tatgaatcaa atttcaaatg
    28621 ctttaacaca ctcagatatt tctacaacaa agatatatgt gaacacacca gatatagttg
    28681 ataaggctgt ttttgaagct tttcagagag gattgaataa gtgtgattga tatgtgttct
    28741 taaatgtgtt cttaaaaaca aaagaagcac tttccaaata attgaaaagt gccagaaacg
    28801 ttgatatata aaggatatta acgttttgag aattgtgaag ctttacgtgc tttcttaaga
    28861 cctggttttt tacgttcgac catacgtgcg tcacgagtaa gtaagccagc acgtttaaga
    28921 gctaaacgga agtcaggatc aacttctagc aatgcacgag cgataccatg acggatagct
    28981 cctgattgtc cagcgtatcc tccgccgttt acgttaacaa atacgtcata tgcgcctttt
    29041 gtttcagtaa cagcgaaagg ttgattgatt acttcacgca agtcagcatg tggaatgtat
    29101 tcttcaacat cttttttatt tacagtaatt ttgccagttc ctggtactag acgtacgcga
    29161 gcaactgcgt ttttacgacg gcctgtgccg atatattgaa cttgtgccaa tgaaattccc
    29221 tcctattaga ttaagtttgt gatgtctaaa acttctggtt gttgtgctgc atgtggatgt
    29281 tctgctccac cgtatacgtt caattttgta aattgtttgc ggcctaaagt gtttttagga
    29341 agcatacctt ttacagaagt ttcgatcaaa cgacgagaat ttttagcacg caattcgcca
    29401 gcagtgattg atttcaatcc tcctgggtag ttgctgtgac ggtaataaat tttgtcagtc
    29461 gcttttttac ctgttaattt cactttgtct gcattgatga cgatgacgaa atcgccagta
    29521 tccaaatgag gtgtgaaagt tggtttattt ttaccacgta gtacagatgc aacaactgtt
    29581 gaaagacgtc ccaatggaac atcagttgcg tctactacat accatttacg ttctacttcg
    29641 cctggtttgg ccatatatgt tgtacgcacg ttattttcct ccaatttaag ttctgatgtt
    29701 tgacatacac ttcgagtttc cggggctctt tgtggggcaa acaataccat ttaacatatt
    29761 acccgctaat ctagtcaatg tcaacgtttt tctgcttgat tgaaaaattt atatgaaact
    29821 ttacttcttt tctttctttt atataaaagg gtcgtttcct gtcaaaatga aaaacaaaaa
    29881 gaggctgtga caaaagtctt gtcacaaccc cttattttga ataaacggtg ttctcctgcc
    29941 aactcctcgg tcacaagtca caatatccat aaaacataag aagctgtttt ttaaaattgt
    30001 ttcttgtgac tcggagctga acggcaggct tacaacctct tggtaaacgg tgttcaatca
    30061 gctgaccctc ggtattagct caaaaaacgg caaaatatga ggaactattt tgacgtttgt
    30121 ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttttcggtc aagttcaaca
    30181 acagttttta cgcgtcgatc tctggcgctt tgcccaaatg agcttggatc atccagatac
    30241 gtttttctgt ggctgttttg aattcgataa agatatcttg tgttggatcg tcgccttctt
    30301 ctccagaaac ttcgattcct ttttggtaaa gatctgctag ataacgatag cctgcaacaa
    30361 gtgtttccaa acgttctgga atagtacgat cccagcggcc aatttcatca gggatttttg
    30421 tgttatctgc aaattcacgc aatgtagaat aaggtgaacc gtctaatgtg atcaagcgtt
    30481 cggatacttc atctaactga gcgttcaaat catccatgaa ctcatccatc attgggtgca
    30541 atgtaaggaa acctggtcct ctcatgtacc aatgtgtttg gtgaacaaca acagacattt
    30601 ggcttagatc tgcaactaat tgatttaata cttctttggt ttgttggaat ttcatctttc
    30661 ttgcctccta acattttctt acacttaaaa taatagtaga ctttcaatga aaagtacaac
    30721 aaaatcattt acctgagaac gattttcagt tcgctttttt atccgtaaag ccaatcattt
    30781 ttcagaatca gctaattctt ctcgcagttg agcaaattgc tgataagtct gtgctaaatg
    30841 cttttgtgtc gaaacaaata atggatagat tttctgatat tttttatgat cagtttcttg
    30901 tactccaact tgctctttca attctatcaa ttcctgtgct tcactgatac tttggatctt
    30961 tccgatgctt ctccagccta aaatcacagc tcctaatgcg gatgcttctg tgacagtagg
    31021 gaaaacaagc gggtaaccca gtacatcagc catcatctga cgaaaaatcg gagattgagc
    31081 aaacccgcct gtagcacgga tctctgtgag ctgattttcc tggcaaactc cttttaaaat
    31141 ccgataaagg ttgagacaga tcccttctag aatcgccttc atcatttctt tttctgtatg
    31201 ttgccgctgt aaaccgataa aactgccaaa cgcatctgcg tcccaaagcg gtgctcgctc
    31261 accaagtaaa tagggatgaa aaatcagacc attggcaccc gctggcacaa aagcgatagc
    31321 cgaaagcagt gcatcatatg gattttctcc tttttcttta gcagattgaa cgacttcctg
    31381 taaaaatgta tgccctgccc aatcaaagat gccagcacca ttgctagtcg ctcctcccac
    31441 tacccaatgt ttctgatcca gaacataaca aaacgtctcc atttctggat ggaactgagg
    31501 ctgagtagta ataaaacgaa gtgctccgct cgttcctacg gtcaaagcgg cgatcgtatc
    31561 accaaaagca cctaacccta gattagaaag cggcccgtct gcccctccca aaacaaatga
    31621 tatatcattt ggcaaaccta gttctgctgc tgtttttgat ggtagacctg ttacttgata
    31681 ggtggaagga aagacttgcg gcaattgctc tattcgaatg ttcaagtagt caagcacttc
    31741 ttctgcccat tggaattcat ggatatcaaa atatccagtt cctgaagctg ttccgtagtc
    31801 gctgatcagt tcgccaaaca atctataaaa gatatagtct ttgataccga caaaataagc
    31861 agtttttttg aataattcag gttggctgtt ttgcagccac ttgattttgt tcaatggtgt
    31921 cattggatga atcggcgtcc cagtctgccg ataaaattct ttgccaagca cggtatattt
    31981 cagtttttct gcttcgtccg ctgctcggtt atctgcccaa gtgatcattc gagataacgg
    32041 tgttttctct tcatccaaca atatcaggcc atgcatcgca gaggagaagg agacaaattg
    32101 aatgattgtg tctgaaggca actgtttaat tacttctttt ataacggtgc agacaccttg
    32161 aaagatcaat tcctgatctt gttctgccat cccttgtgtt tcttggatca aagggtagcg
    32221 aaaataagcg gatgctgctt cttgggcttc atctgtgaaa gcaactgctt tggtttgtgt
    32281 ggttccgata tctaatccga ttgaaacgtt cattttttca cctcaaattt tatgtatgta
    32341 caaaaattta gtttaagctt gcgtatcttg gttttgaaaa gggggagatg gcgtgttact
    32401 ttattttatc attggttgct gtatcggatc atttttatgt ttaaccgccc agcggattcc
    32461 gttaggtcac tctattattt atcctagatc tcattgtgtc aaatgctgtc gttcgctttc
    32521 ttggtatgaa ttgattccga ttctctcgat catcgtccaa cgatttcgct gccgctattg
    32581 caggtgtcgg ttgcccttct actatttgtt ggcagaagcc ttatgtggtg gtctttttgc
    32641 ctggttcttt actttttctt ccgcaagaaa tttttcgacg ttcatatgga tgctttcggc
    32701 tttgctattt tcgttgatgg atttatttta cttcatggtc cattctcaca cgctattctg
    32761 ttcttgggca attttatgga ttttttggtt gcaggccggc gtcttccaat ggcaaagtgt
    32821 gctgttaacg tttatcgtag gcggtgtgtg ccttcgctat ggtcaatcct atctcggagc
    32881 tggcgatact ctgttgatgc tttcttggag tggtggcttg tctctagaag agttgcttca
    32941 gatcttgttt cttgctagtc tgtttggaat atccgttttt tctgtttact ttatctgtca
    33001 taaaaaaaag ttggaaaagc tgcctttcat tccttttttg agcagtgcgt tgctgattgt
    33061 ccttcatctt aggtattgaa aaaaggttgt gacaaacgac ttgtcacaac ctctgatata
    33121 tagtgttctc ttaccaaatc cttgtgactt ggagctaggc gacaggttca caacctctcc
    33181 actaaatggt gttcaatcag ctgaccctca gtattagctc aaaatgctgg ttttacaacc
    33241 tctgatatac ggtgttccat aagtagcttt ttcggcttct tatttcttga cgccccaatg
    33301 tcgacgaagg aaacctgcac gtccagaacc ttctcgataa agattgtaat ggagcttgtt
    33361 tttcttgtaa taatcctgat ggtaatcttc tgcaggataa aattcgcttc ttggttcgat
    33421 ttttgtcacg attggcttat cgaatcgccc gctgtcttgc aatgcttgtt ttgaacgctc
    33481 tgcaatattt cgttcttctt catcattata gtagatcact ggacgataag aatcaccgcg
    33541 atcagcgaac tgtcctaatg cgtcagtcgg atcggtttgc tgccaataga tttccaccaa
    33601 ctgatcataa gaaataactt ctggatcata ttcgatctct actgcttctg tatgccctgt
    33661 tgtgcctgtt gttacttgtt cataagttgg atttggtaca tgcccgccag tataaccgga
    33721 tgtcacagaa atgatccctg gctgctcttc aaatggtttg accatacacc aaaagcatcc
    33781 gcctgcgaaa actgcttttt ttgtcatata aaatccccct tttttctcat cataccttga
    33841 tttccaaaag gaggataacg ttttgcttgt gacagaaaaa acggaaacta ttatatacgt
    33901 gatacggctt ctgataaatg ccaaaacgaa cgtacttgat caaaaacggt taaatcagga
    33961 atcacatatt cagcagaagg aagatttatg ttttcaccta ttcccaatac atgcatgtta
    34021 ccagccaaag ctgcttgaca accagcttct gaatcttcaa tgaccaagca tgcatttggc
    34081 ggtacattca attgctgtgc tcctttgaga aaaacttctg gatcaggttt tgcttttgat
    34141 acttgcgtgc cgtcaataag gacatcaaaa taacttgtca aattcgtttt ttctaaaatg
    34201 aggcgtgcat ttttgcttgc tgatcccaat ccgatcttga tgtttgtttt tttcaagtac
    34261 tcaagaacag ctattgcacc aggcaaaacg gatgtttcat ccattttttg aatcgcttga
    34321 agatatagat tgtttttttg ctcagcaaag gctgatttct ctttttctga gaatttctgt
    34381 tccatttttc caaatgacag caatcgctct agtgactcgg ttctggatat tcctttcagt
    34441 tgctcgtttt ctttctcagt gaatggaatg gacagctggt tagctaaaac cagccacgct
    34501 tcgtaatgat aacgagctgt gtctacaaga acgccatcta aatcgaatag tgctgctttg
    34561 attttcatcg attacctcct tgaaaatgaa cgacttgaag taaagatgcg atgtttttag
    34621 ctgtgcgatg aagattatca gtggcttgct gtaaagcttc ttgtaaaggc acagctgcag
    34681 gaaggatcgg aaaaacggcc gaaatgccaa catctgctgc tttttcgctt cctttaccaa
    34741 cacttccgca aatagctatc acaggaacgc cctctggtac tcttctagca atcccaactg
    34801 gcgcttttcc ttttagactt tgtccatcta gacgtccttc gcctacgatt acgaggtctg
    34861 cttgctgtac ccgtttatcg aactccagta aatccaatac gtagtcgatg ccagagacta
    34921 ttttccctga agcaaatgct aacagtcctg cacccattcc accaccagca ctactgcccg
    34981 atgcctcgat tacttgaggg gcaaatctca tataaaaatc atgcatggct tggtcagcca
    35041 ctgctaattc attttctttc aagccttttt gtggtccgaa aacaaaagaa gcgccttctt
    35101 ttccgcataa aagattagta acatcagtaa tgacagtgat tttggcttta atcagctcaa
    35161 taggcacgtt cgttttatca actgtagcaa ttttacctag attttctccc agtgcttcta
    35221 ctttttgatt atcttgatcg taaaaagcat aacctagacc ttctgccatg ccgatgccgc
    35281 catcgttggt actacttccg ccaacgccga tgatgatgtg tcgaacacct tgttgcaaca
    35341 attgtcgtat cagttctcct actcctcgcg tggataacct aagtggctgt tgttggttta
    35401 gtgggacatc tttcaatccg acgatttctg ccatttcaaa aatcgcagtt tgaccgtctg
    35461 aagcgtactg gatttctttg tttttgccaa atgcattagt aacgactgct gtttgccgct
    35521 tcaaccttaa actgtctgct aatgatgaaa ccgttccttc tcctccatcg cctataggta
    35581 gcagatcata ttcagcctga acaaaaactt cttgaaaacc tgattgaatg gcttgtgctg
    35641 cctctaaggc tgtcagactt tctttgaaag aatctggcgc aatcagaata cgcattattt
    35701 tcccctccgt tccaagatca gttctttttc cagctggtat tggtctccat tgacaaaaat
    35761 aggtaatgtt tctttcgtct ctttttttat tactatttgt ttttttgtca aaacaaatag
    35821 caacctgtga tttttccaaa taaaaggaaa agaaattttt tcccatgctg aaggaagtct
    35881 tggagaaata gaaagagttc cattcgttac tgtaatacct gaaaaaccaa atataactgc
    35941 tagccatagt gctcctaatg aagcagcatg aattccgtca tctgatgaat gcggatctgg
    36001 tcctaaatca atcaggcacg cttcttggaa taaatgatac gcccattcca tttcttggat
    36061 acggcaagcc tctatggcgt gaatcgcttt actcaacgat gagtcgtgga ttgttttgtt
    36121 ttcatagtaa tgcaaattct tagaaacgat tggttgagaa aaatactcag gtaataagta
    36181 taacagcatg acaagatctg cttgcttgct gacttggagt tcatttactt cgctacgggc
    36241 ataatcaagt aaaatagact ggctgccagg acgttctttg taagtggtca aatcaataac
    36301 tggcttactg agatacgtgt catcttgcgg aatcagctgt tctttgttag gcactggcaa
    36361 atataactta tctaaaaaat tttgacattc tttcaaaaaa agcggatcgc cagcttgata
    36421 tttaacagct aaggccacat tataatgcgc cagatagttc gtatatgcat tattgtcgat
    36481 atattctgtg tattcatctg gaccaatgac tctgtgtatc tctaaatgat catcgacttt
    36541 tacagctctg ctcagccaaa aagctgctgt ttcatgaatg agcgtttttc cctttgtttc
    36601 taaaaacgat tgatcctctg ttgcttggac atactcttga acggcataag caaggtctgc
    36661 actgatatga tgttctgcgg cagctgaagc aacttcttgt cttgttccgg ttttgatatt
    36721 caatgcagca aatttgggtg tctcttcctg accagtgtgt gcgctttccc atgggaaaag
    36781 tgcaccttga tagtgataat ctcttgcttt ttgccgtgcg ccttctaatc gatcataacg
    36841 gtacagcagc aaattctttg ctacttcagg aaactgatat aaaaagaaag gcaggataaa
    36901 aatttccgta tcccagaaaa catgcccttt ataaccttcg ccagttagtc ccttcgcccc
    36961 tacactaaca cttggatcat tgcggggagt cataatctgt aaatggtaag cagcgaagtc
    37021 catcgctaac tgatcgaaag aagaatcgcc ttggatcgtt atccgatgct gatgccaaaa
    37081 atctgtccaa taagacatcg actgctctaa aatttctgta taactaacgc ttttctgggc
    37141 ttgggcaata cttttcttaa acgggtctgt ttgctgaaga gaggtagaga gcacgatgat
    37201 cttctccagc gtaaaggacg tattttttct gatcttttct gaaaaggaag cggtgatccg
    37261 gcgatttttt gcttggaata cacctgcttt attgaattta gctgcaatcc caatatcaat
    37321 tttactttct gttgtctggt aggatgcact gatcaaatcg tcaccaaata ctcgcagttc
    37381 tttttcaacc aactgctgtg taccaaaatt ggtttgctgt ccatcgatac ccgttttcac
    37441 atctacttgg cagtcttcgg tcaacggctt gatgaccact ttcagggctg cctgatggag
    37501 attattttga tcagcaacac gacaagtaat gacttccact ttttttcctt gagcagtttc
    37561 aatgagcgca tagcgtttaa gctctcccat ttctaaatct agtgcccgct cgtatgcaag
    37621 aactttcatc gtctcaagag aaaaatgttc accgtcgatt tttatttccc atctgatgac
    37681 atctgggaga ttcactaact cggctgattc tgctcctaaa ggtcggttat atacaccagc
    37741 tgcaaacatt ccccttactt gattccgata gtcttcctcc tggcttcctc tgataccaaa
    37801 aaatccattt ccaatggtca tctgactcgc atacttattg atatactcta aatcaaactg
    37861 tttttcttct actttcataa ggtaataact tctcctgttt ctgccgaacg atataaattt
    37921 tccaccagat tctggatgac atatccttgc cagccatctg caatcatctg agtctggtca
    37981 ccgagcgccc gattcacaaa atgagccata cttagttcat gtgggtcatt aactggcgtg
    38041 gtcttttgta aggaaaccag ctttccatct acatcttcga atagttcgcc tggatacaga
    38101 ttagcacctg cctgatcgcc acaaaaagcc acattcagtt tgtcttctct gatattcaaa
    38161 gcaaacgaag tattcagttg gatgatcttg ccgttttcta attccagtgt gccaaaaatt
    38221 gcatcttcta ctgtgaattt gcttggatcc catttcccga atttccctga attttttttc
    38281 gtaccaattt tttggaagct gtacgcccga acgcttttta ctttaggaaa tccagtaata
    38341 tacatcgctg tatcaagcat gtgaatgcca taatcgatca aaggcccgcc tccttgaagt
    38401 tctttgtcga taaacgttcc ccagcctgga acaccagacc gacgaagcgc attcgcttct
    38461 acgtaataaa tatccccgag aacatcaaga tgattcttga tcattgttgc ttcgtcagaa
    38521 aaacgatgct ggaatccata agccaaaatc tgcccgtttt tatcggctgt ctctgccatc
    38581 tttcttgctt gatcagctgt catagctggt ggtttctcgc acatcactga agcattagct
    38641 tcgagggcag ccaatacatg ttcgaagtga aagcgattgg gtgtacagac actcacgata
    38701 tcgggagaaa tctcattcag catttgtgta actgatgtat acgcatgaga aaatcctgta
    38761 cgtcggcaaa agtcttctgc tcgctggctg actggatcaa ccacagcggc caattctaag
    38821 acatctgatc ttttttgata ataagcagca tgtacttttt ctgctacttg tccagcgcct
    38881 atgattgcta ctttcttcat ttatttctct ctcctttgct tatttactta ttgttattct
    38941 ttctctattc tttctctaaa tcatttaaat gacgcgctgt ttccagacag tttttcaagt
    39001 attgcaatga ttcctgataa attttcactt cattttctcc tcgcaaacga cattcatata
    39061 ccatataacc ttcatactca ttttgaagta gcgtttgaaa atggcgagta aaatcaattg
    39121 cgccagatcc tggttggtag cgatgattat ctgctaaatg gatatgaccc acatactctt
    39181 tttgcgtagt caatgctttt gaaatatcat cttcttcaat attcatatga taaaaatcag
    39241 cgatgatctg cacatgtttc aattgattag agtcaatgta atgacgcgca tccgctaaca
    39301 aattgatcat atggtcttgg tatcggttca aaggctccaa ataaataacc gtacctgttt
    39361 cttcggctac tttatctaaa taacgaagtg attcgctgac tgctttatag tctccttctt
    39421 tgcttcgggg tgaaaccatc ggcggtaatc gataagtgaa cattccccaa gctgctggga
    39481 taacgattcc tttgcctcca acctcgctta gtgcacgcaa aatctcagca atttgacgta
    39541 caccattaag acgccgttct tcgataaagt caccgatcca cccatcgtag ccgttacaag
    39601 ctgtcactac tgggatccct gtttctttga ttgcctgttt gactgtgtcg atattttcta
    39661 ttaacaactt accgtcgatt tcgtattctt caaatccaca gtctttgata aatctaaatt
    39721 tttctaaaat gtctcttggg aaaaatgctt gatcttgcgt accgaatttc attgttctcc
    39781 tcctcggact tgaacgccca ttttgatact ttcttctggg tgctggtcta cataacgcat
    39841 gtaagcttct gcgctttctg caaatggcac aactggatca atgatttctt cacaatccaa
    39901 gtaaccgttc atcaataatt cccaacatgt gtcctcgatt cgtttacggt tccatcttgg
    39961 gtaatctggg tttggttcac tagatgctcg agcaaagacg atttttgcat tattgaaatg
    40021 tgcttcgcga ccaagattaa acccagctgg aaaaggttta gcaaaggcaa cataagaaat
    40081 gatcccgcca tagccgatcc ctattaaaga agcttgtaat gcggcagcac tgccactcgt
    40141 ttcaataact gaatctactc cacgtttatc tgtcaattcc tttagacgaa aaccgacatc
    40201 tgctttcata ggatctagcg tgtaatcggc accgtgtttt tctgctaatt ctctacgctg
    40261 cgctatggga tcgatcccaa cgatggtagt agcacctgct tttttagcta attgaatcgc
    40321 aatttgcccg atagcgccta aaccgaagat tgctacagca tcacctgctc gaacatgtgc
    40381 ttcccggata ccacctaaag cgaattgagc aggatcgtaa catactgcat ttttccattg
    40441 atccaaccga tcaagtttac gtagtttata attatcaaca ccgtttgtaa tcagcgtttc
    40501 acgaatgggt ccataagtgc agactaactc tcctttttga tactctgtca ccaaactgcc
    40561 cgtttcttcg atcgtaccga caatcatatt tcctaattga aattcgccaa ataccacacc
    40621 gcgttggctt ccttcttctc ttggcaagaa taggcgccat tcttctgaaa attcttcatc
    40681 gataaatgga cttaatcctc taaagtcgac aatttctgtt cctggttttg gagtagcgaa
    40741 aattactcgg atacgtactt cgttcttttc gatctcacgg tcttcgtaat ctttgaatcc
    40801 ggctattctt ggttctaatg caactaattt tttcatcttt tctttcctct ttcttttctt
    40861 tttatccttt tacgccgcct tcgactaaat cacctttaat aaatcgttca gataacgcat
    40921 acataatgat gactggtaaa gcggtcacca atgaagcggc catcattcgt ccccaaatgt
    40981 agtctggtgt actgaataat gtgttcaaac cgattggtaa agtaaatttt tcggaggtcg
    41041 acaggaaaat cgaagcaaat aaataatcat tccacgaaac cataaagcaa taaacgaaga
    41101 cagaaatgat tccagaaata gccaatggta taatgatccg aataatgatc tggatccggc
    41161 taagaccatc gatcattgct gcttcttcaa tatcagtcgg aatcgtgtca aaataacttt
    41221 ttagcatgaa tatcgcagta ggaagtgttt gaacgaccat cgtgataatc agtgctgtat
    41281 tggtatcata caaacctaga ccggaaatga ttttaaataa tggaacaacg atcaaaatac
    41341 cggaaaacat ataaacggtg taaaagctag cattgatttt cgagcgagct ttgaagcgta
    41401 gttttgacaa agcgtatgcc ccgctgatcc caatcactac tgcagttacg gaggctatca
    41461 aggagatcac taaactattt ttgaaataca tgatgaatgg aaaaatctca gcattgaaaa
    41521 tatcctgata atgttcaaaa gtccattctt tcggaaaaat agtttgtgtc gttgcaattg
    41581 cttcttgtga atccttgaat gatgtcatca gcatgatcaa aaatggaaac aaattgacag
    41641 ctaatagcaa caaaatcgtc gcgtaaaata agattttcct gctcttttta tttttcattt
    41701 caagtccacc ttctttcgtg agagcaagac gaccgctgca ataatgatga acaatagaat
    41761 cgatatagct gctgcttttc caagatcatt gaacgcaaag gctgtctgat acaaataaac
    41821 gcctaagata ttgactttat tcgtcagtag atagacatct gtaaacatat agaacatcca
    41881 gatagatcgt aaaacaatca cagttgctag tactggtttg attgccggca gtgtcacgat
    41941 cttgaacttc tgccaagcat ttgcaccgtc catttcagct gcttcataaa gcgttttgtc
    42001 gactgtttgc aaaatagcca aaaatgaaat aaacgcatac ggatagtatc tccagatagc
    42061 aaacaaaacg accataaaga agctagcaac tggctggtcg aaccacagcg gtgcttcgtt
    42121 gaaaagctgc aacctatcta ctaacagatc attgacgatt ccataactat tattgaacat
    42181 atatttccaa gcaaaaatca aagaaataga tggcgtaaca taacttaata aaatcagtga
    42241 acgcaccgtt ttcctaaatc tgaacttgcg gttaagcaaa atagctacgg ctaatcctaa
    42301 agctgtactg cctgctacaa ctaagaatgt gtaagcaact gttaaaccta aagaacgata
    42361 aaattcacta tcacttaaaa tttcaagata attgcttaat ccgataaaaa cggcgtctga
    42421 gttaggattc agcggcatat cgaaaaaact gatctgaata ttggaaagca ttggaatcag
    42481 cactaaaaca gctaataaaa gcatgcttgg tgctaaaagt cccatggcta attttaagtc
    42541 agaatgtttc cttggacgat tcattgaaac ctccccctga taatctgcca atagctatct
    42601 acttttttcg aacaccattg gcagaacttc ttgttatttc gtattttttt gtgcgtcagc
    42661 taaagcttga tcgacatctt cgtttccgac agttacttgg ttcactgctt ttccgatcac
    42721 acctgagctt gtgatatctc ccattttcgt gaaattcttt tccccgacta gtccaaagac
    42781 ttgtacttca tcaaaagcac tagcaatctc agatggcagc tcgccaaaag acttgatgac
    42841 ttcattttct ttgtaaccat ccagttcgac tacttgttta ttcactggtt gagcaccacc
    42901 aggagacatc aagacccact tttccatgtt tttcggttga gataagaaag ctgtgaatgc
    42961 ttcagcagcc tctttttgtt tctcgtctag accagaagaa atcgtcaatc cagatactgt
    43021 gccatagaca gcttgatctt tttgtgttgg gatagcaaaa ccgatatttt ctgagttccc
    43081 cgcttcataa acagaaggca aaatgtaagt tgaatagatt gccattggtg ctgttccatt
    43141 catgaaagca tctttgattt ctgtcacatc attagagcct ggcatcgtgt attgggataa
    43201 gttttgataa aacgacaatg cttctctcat cttttccgta tcgatcgtta cctcgccttc
    43261 atcatccaaa acatttgctc catttgacaa agcaaattga gagaacgctt gctcactcat
    43321 tgttccttca gcagtaggca tagcaattcc atattgttta ttctctttat ctgtgaaatg
    43381 ttgagcaatt tttaaaatat catcccaatt ttctggttct gaaaaaccag cttctgataa
    43441 tttttctttg tcataccaaa taccttgaac ccagccgcta attggtgcag caatatagct
    43501 ttttccatct tctgaacgaa caaggttttt ggctccgtca taataattat cttctccagc
    43561 ttcctcgata acgttttgga ctgccgtctg atcaatcagt tcatctttat ccatcacttt
    43621 agcaaaatcc tgactgactt ccattacttc tggtaattta ccggaacgag ctaacgtaac
    43681 gatttttgta ttgaacgctg attcttctac cggcacttgc ttgattttga tgtctggatt
    43741 ttctttttca aaatcagcaa ccagttcatt gatcacactt aatcgttcct gttcgacaga
    43801 agagtgcatg aattgaatcg ttgtctgttg tgattcgtta gagctttgac tttcaccaca
    43861 agcagtaaga agaaatccag tagctagcag acaggtagta acaataattt ttttcatttt
    43921 tttccacctc ttattttatt attttcagcc acatatattc atacggctgg aatatcaact
    43981 ttcgttcttg actgcaatgg ataatttgtc cagaaaacag gtctaggtaa tctccttttg
    44041 gtaaaagata acctgctgat tgattggaaa aattatggat aactaagact ttctcttcct
    44101 tcgcttcgcg aataaaactg ataaaagaat ctgctgagtc cagtaagtaa atcaaactat
    44161 tcggatggaa ggctttttct ttttttcgaa tagatagcat ctgggtcaat tcaccatata
    44221 ctgctgttct taatggatct gtctgcaact ctttctcaat atctgctaac aaaaattttt
    44281 gccgattgat ttttcgattc tctcctgttt tttctacgcc tttgtaatca tttcgacttc
    44341 ccagcacact ttgaatataa attgccggca cgcctggtaa cgttaccaaa aatgaatgag
    44401 cagctaaaaa gcgtttgctt ctccattccg tcgtatctgt tggagaactt aacgcatcta
    44461 gatacgtagc gtttatctca tatggactgg tcgttccatc tgggtttttc ttgtagttta
    44521 ctcgggcacc ctctttcttc agctgttcca ctaattcaag gatcgttttt tccggcagaa
    44581 tacctcgtac cggattcaat ccgataccgt catgggaggc taaaaaatta aaaaaagttg
    44641 tttttttcga tgcgaaatcg atggttttta accattcttt caacttactt gtattacccg
    44701 aacgaatcgc ttgaagggtc aaaggtggta gcgaaaactg atagaccatc tgtgcttcat
    44761 tctcgccatt cccaaaataa ctgatattat ctttgtgtgg cacatttgtc tcagtgatca
    44821 ataccgttcc tggagctgct tcatcagcga ttgcacggaa taattggatg attttatgcg
    44881 tttctggcag gtgtatacaa gaagttccta gttctttcca taaaaatccc acagcgtcca
    44941 agcgaataaa tgaagcgcct ttttctaagt aaaacaaaag aatatcgatc attttcaata
    45001 aaacttctgg atttttaaaa ttcaagtcta cttgatcttc gctaaaagtc gtccatacgt
    45061 gtttttcttt cccgtttttg tcaatgaacg ttgtaagtaa cgggctggtc cttggtctag
    45121 tcacgctgct tgtatctgta tccagtggca aatcaataaa aaaatctttg tacgtatcat
    45181 tcaagtttaa atattcctga aaccaagtac tttcggcaga catatgattg cagacaaagt
    45241 cgaacattaa ccgtgtttcg cttctcattt cttctatatc ttcccatgtg cccaaacggt
    45301 cgttgattgc ctgataattt acaacggaga aaccatcatc agatgtatat gggaaaaatg
    45361 gcaagaaatg tacaactcta aatgtatctg ccaaatactt ttgataaaat ttcttaaatg
    45421 tacgcaaagt tggttgctgc tcttcaaaaa attgatcccc ataagtaatc aagaaaatat
    45481 cttcttcgtc ccaatgcagt ttgcctgcct ggcctttctt ctgctgataa acactgactt
    45541 tttgttcaat catagaggcg gttttctcag acaaatctga gtcgcgataa atcgcttgta
    45601 aaagttttgt atacatcgga ttcccctttc ttttttggga gcgttcccaa cgatttgatc
    45661 atattatgga aaacgcttac tgtcaatagc atttttttaa ataattttga tatacacaaa
    45721 aatacaaata agaaagctta tgtatcaata attaatagct tcttaaaaaa atatggtctc
    45781 tcatgatcat gcaagctatt cctaaatttt caaaatcata gacaaaaaag aatgtctatt
    45841 gtactatgga attattctaa aacgctccca aaaaggaggt gcatataaaa ttgaaaccta
    45901 ctatttatga cattgcgaaa aaagctaatg tctcaaaatc aactgtctcc agagtattaa
    45961 ataaccagcc aaatatctct gataaagcaa aagcaagtgt acttcaagca atcaaagaat
    46021 tgaattatga accgagcaaa attgcaagag gactgtcagg aaatggtttt gacgcgattc
    46081 tagcattatc tcatcgtact tctcatacga cctatggcaa tccttttttt tcagatgtta
    46141 ttcaatccat ttctgccgtt tctgaacaaa ataacttcga cttgattcta caaacggcaa
    46201 aaacaccaga agaagagcta gaaaaatgcg cgaaaaagat ccaagaaaaa atgatccgag
    46261 gaattattat tttaagctct acgatggatg aaacacttct ttttgaattg gatcaatata
    46321 atattcctat tgtggtaatc ggaaaagtcg gaaacgacta cacgaacatc tactcagtgg
    46381 acacggataa tttccaggat agttacgatt tggtggaaac actgatcaag aatggacacc
    46441 gtcatatcgg ctgtctccat gctccgacag aagtttatgt atccaatgat cgtctcaatg
    46501 gttaccgtca atgcttattt gatcaccacc tagatatccg taatgaatgg attatagacg
    46561 gcggctttcg cttagaagat agcatggcag caatgaatga tttaatgaag ctacgagaaa
    46621 gaccaactgc gattttttct accgatgcat taaaatcatt gagtatttac aaagtggcag
    46681 aacaatctgg aaagcagata cctgatgatc tttcgatcgc aggattcagt gatcccttat
    46741 tgtcctccct attttcgcca cagttgactc ggatagacat tccgacaaat cagttaggaa
    46801 tcaaagccac agaactttta ttccaactaa tccaccacca ataccctgaa gaaaaaagaa
    46861 tcattctttc aaccgagatt actctcacag attcgatcca acaacaagaa atgtagaaca
    46921 gaattataaa agaacagaaa cactgaggat aagtcagtta ctctgttctt ttttcaatca
    46981 ctcatccctc gttaccccct cccggtattt tagcgaattt ttcttatttt ccatctgttt
    47041 tagatagctg tggataacta taatgtttgt gttaaactat tttttccaca gggaagaaaa
    47101 aactattttt aaccacgttg tgtataagtt tttatagttc gtttttttaa acacatttac
    47161 ccttatttca atccatgtta tttggggata cttatctcta aaacataaat agaataacag
    47221 acatgtctac tggttttgtt ttattcgagg aattattcaa taatagctag caaaatatta
    47281 aaaataaccg ctctatcttc ccatcaattt attttgcatt caaaaaatga tctatcttta
    47341 cttttgttga ttgacttctc ttggaagatg atcgttccga taccttaatt ggatacgaac
    47401 cgatgttgtt aagatccttt cccacttttc ttctagttag acaaattatt ttagacaaac
    47461 caaaaaagag gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt
    47521 tgattgttca ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca
    47581 ttttcgcttg ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta
    47641 ccagctggga tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc
    47701 ttaccgcgga tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat
    47761 aagaaactat ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt
    47821 gcaggaacgc ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct
    47881 aataatgtac ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc
    47941 atttgacgaa ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa
    48001 acacgttgta cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac
    48061 aattgttttg gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct
    48121 tctgctactt tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct
    48181 ttgattgtta cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct
    48241 gttacttctg tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca
    48301 cgaggaagac cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc
    48361 gtcaactgag taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg
    48421 acttcaacgt cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt
    48481 tttgtattac atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt
    48541 tgttttgcga tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca
    48601 ggatgacgta cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg
    48661 atttcatttc cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct
    48721 tcacggatga ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg
    48781 gcagtcttca aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt
    48841 tccaagacgg atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca
    48901 ttaggagcgg ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca
    48961 cgagctccag aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt
    49021 ttttgttgga tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg
    49081 tcatctgtga tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca
    49141 ttttcaatga tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata
    49201 ccggcatgtg tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt
    49261 tctgttactt tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg
    49321 aacggtaata ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca
    49381 aagtatttgt ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg
    49441 aattcaggag gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt
    49501 tctttttgcc attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt
    49561 gaatgtaaat gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag
    49621 accattcctt cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc
    49681 atatcttgag aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca
    49741 gctaacatca acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc
    49801 atttggtctc catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt
    49861 gcgcgtcctt gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt
    49921 gcccggttca gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg
    49981 tcttcttgac gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga
    50041 acaagctcac gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca
    50101 cattggtaca tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca
    50161 cgtttaccta gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga
    50221 gatttcaatg ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc
    50281 aatgcatcta ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca
    50341 ttcaagtcta acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca
    50401 ttcaagtcac ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt
    50461 ggaataacag ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat
    50521 gcttctaaaa tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc
    50581 aattcttctt tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta
    50641 atcgcttctg cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga
    50701 tattcgcgtt ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact
    50761 acatatgaag caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga
    50821 cccatacgtg aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca
    50881 atatgtccca tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca
    50941 caaacgattc ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt
    51001 gtaggaccaa aaatccgctc acagaacaac ccttcacgtt caggttttaa ggtacgatag
    51061 ttaatcgtct caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa
    51121 gccaaaccta tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat
    51181 ttcatttaat ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg
    51241 gattagtcta aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct
    51301 tccacttgtt cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt
    51361 gtcaaagcat caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc
    51421 tctgtttctt cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc
    51481 aatacgcgga atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct
    51541 tcgtatgttt tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa
    51601 gtataagccg caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca
    51661 ccaaattgag ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa
    51721 cgtgcatgca atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca
    51781 gacacacgtc catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg
    51841 ctggccatac cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat
    51901 actggcgttg ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat
    51961 acttgcccga tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt
    52021 gttccatcag gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta
    52081 tttccgtgac gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa
    52141 acacgaacta acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt
    52201 ttcacatcgt gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact
    52261 tcacgagctt tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt
    52321 accccttttg gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata
    52381 cggatgatac ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg
    52441 cgtgtgattt cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata
    52501 tggatagaag tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca
    52561 tagttgtacc cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg
    52621 ccttgttcca tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa
    52681 tgaacgattg gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca
    52741 tcgtatttat ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat
    52801 tcaacgacac cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat
    52861 tccataccag taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc
    52921 atgttggcac ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct
    52981 gtcgcaacgg caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat
    53041 acttctaagt tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct
    53101 tcattcaatg ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag
    53161 tagtcgattt ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg
    53221 ataaatccga atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt
    53281 gggccttcag gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga
    53341 acttcataac cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt
    53401 ttgtgtgtca attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa
    53461 ccaaagaatt ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc
    53521 aatgtttcag tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc
    53581 ccgatacgga attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag
    53641 tggtcgatat catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa
    53701 gcaatgatgt cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc
    53761 acgtttactt cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt
    53821 ggatctgtga ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca
    53881 atgaaaggag ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg
    53941 atctcgcctg tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt
    54001 ttcaaatcta attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt
    54061 ggatcgaaga aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct
    54121 ggacgcaaac gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt
    54181 ttatgcaagt ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag
    54241 atcgtatcat cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt
    54301 gtacggtcga tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct
    54361 ccacggttag ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca
    54421 tggaaataga cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg
    54481 atgatgaatg ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct
    54541 ttgatttcgc cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag
    54601 tttgcatcat gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct
    54661 acaaattcca aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt
    54721 tctctaagtc cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt
    54781 ggtaattcca acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat
    54841 tttactacgt gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc
    54901 gatcaattgt tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc
    54961 ggcttttagg caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc
    55021 ttctactttt gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc
    55081 ttcatcagat agtttttgca acttcatata ctacacttat tttctataaa agtcaacact
    55141 tcctggcgtt tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc
    55201 aattatctga caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc
    55261 attttgtgga ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa
    55321 taacttttac tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta
    55381 aacgacagtt tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag
    55441 acaaaatgct aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt
    55501 tttcaattta ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg
    55561 tttcccaaaa ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct
    55621 gccctgctac tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat
    55681 ggacaaaaaa caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat
    55741 aaaggattca aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc
    55801 gattatcaaa atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc
    55861 aaagaagatc ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat
    55921 gaaacaacca ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta
    55981 gtcacatcca atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc
    56041 gaaaaacaaa tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg
    56101 tataacaacg gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa
    56161 aaagcgggcg tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg
    56221 acgaaaagtc tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca
    56281 agtgcggcaa gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc
    56341 gcagttgatc aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg
    56401 ggcaaacaaa cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt
    56461 cctgtcgaaa cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta
    56521 ggcatcaatc cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa
    56581 atttgctctc tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga
    56641 ttggtgtata cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct
    56701 ttcctttagg tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag
    56761 cttgtcttct aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca
    56821 caaaattgaa aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg
    56881 tcacatcccg tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc
    56941 attgggtcga gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt
    57001 ttgctttgat cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta
    57061 ttcgtgcaac tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata
    57121 tgaagatcat cggttatatg atttctaatg gatgcattgc tctttctggc tccttacttg
    57181 cgcaaaacaa cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg
    57241 cttctattat catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga
    57301 ctgtcgtttt aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg
    57361 ttgaaccaag tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc
    57421 cgcagatcca gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa
    57481 tgacgtctta acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa
    57541 tcatgtcctg aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg
    57601 tggaaatggg gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga
    57661 tcagggtcaa attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc
    57721 gaaatccatc agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt
    57781 agaagaaaat cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt
    57841 caaaccacaa gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga
    57901 aaatcgttta tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt
    57961 attgatggcc acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct
    58021 tgaccctgct acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa
    58081 tctcactgct tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact
    58141 gatcatgctg catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct
    58201 cacagtgccc caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga
    58261 tcaagtactt ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct
    58321 cttctttttt ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga
    58381 aaactatttt gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat
    58441 gacaacgaaa gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga
    58501 aaagttggca cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt
    58561 ggaaaaggca ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga
    58621 cgtactagat gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt
    58681 acaaacgatg ttgacttctg aatctacgat gaaagatgcc aaactggcag cagctcaagg
    58741 agattctgtt tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg
    58801 acggcctttt ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga
    58861 caaacggttt gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc
    58921 cattgaatct gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga
    58981 tggaaaaaaa gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat
    59041 cagtcatatc atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc
    59101 tgatctccaa gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa
    59161 tcagctaatc agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc
    59221 gttggacttc tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa
    59281 aggaacgact tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca
    59341 aacaaatgaa cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac
    59401 gcaataatta tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga
    59461 attctcttca aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa
    59521 aggtattcta aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa
    59581 aacaagagag tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat
    59641 cttatagata gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg
    59701 taagttcttt tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc
    59761 acctgaccag agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg
    59821 actggtggtg tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt
    59881 aagtaagaaa tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga
    59941 ttagcttgga tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct
    60001 atcgtcatcg gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct
    60061 aaatcagcaa tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat
    60121 ttgatctgcg gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct
    60181 tcaaacaaac gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa
    60241 taatccattt attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata
    60301 cctacttata tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat
    60361 agtttggaga ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat
    60421 atgaacgttt cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga
    60481 ctaggagaaa aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa
    60541 aaaagggcaa gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg
    60601 gccgtttcag cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact
    60661 ggtaactatg gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca
    60721 actgtgaatg cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat
    60781 tttggtacag agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact
    60841 gattctttcg ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc
    60901 gttggcagta acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg
    60961 cttgatcact taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat
    61021 ttcgagcagg aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca
    61081 gctaaaacat atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact
    61141 gatttatcag ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat
    61201 acgccacttc aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt
    61261 tcttctgcat tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca
    61321 gaagattttt cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca
    61381 atcagtgctc cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa
    61441 aaccaagacg gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg
    61501 gatcctaacg aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt
    61561 acagatacat ctaccacaag tgatagctca gattcatcaa cttcaagcga cagtacagat
    61621 actacctcta ccacaagtga tagcacggat acatcagctt caagcgacag tacagatact
    61681 acctctacca caagtgatag cacggacaca tcagcttcaa gtgacagtac agatactacc
    61741 tctaccacaa gtgacagcac ggacacgtca gcttcaagcg acagtacaga tactacctct
    61801 accacaagtg atagcacgga cacatcagct tcaagtgaca gtacagatac tacctctacc
    61861 acaagtgata gcacggacac gtcagcttca agcgacagta cggatacatc taccacaaca
    61921 agtgatagca cggacacgtc agcttcaagt gacagtacag atactacctc taccacaagt
    61981 gatagctcag attcatctac cactacaagt gatagcacgg acacgtcagc ttcaagtgac
    62041 agtacagata catcctctac cacaagtgat agctcagatt catcaacttc aagcgatagt
    62101 acagacacgt cgtctaccac aagcgataca cgtcatgcaa gtgtctcctt ctcaaaaaca
    62161 agttcggata acaggacgaa ctttaactta actaataaaa caagcaatga caatggaaac
    62221 aaatcaaaaa gagcacttcc taaaactgga agtcaatcga ataattggat aacactcgca
    62281 ggcgttatat tacttgtaat cggtttaaga atcactttta tgagttataa gaaatcgaaa
    62341 aggtaaaaaa atattcaaga taaaaaagag caacgtcgct aacggacgtt gctctttatt
    62401 tttgcttttt atcataaata caagaactac aattcaattt gtcaaaaaac acgtagattt
    62461 ccttctgaga aatttattta cgtatcgaac ctttccctat tcagtctttg cgatccaaaa
    62521 tcagttgggt aagggaaaag agattctctt ttgttcgaag agaggtggca ggtaatttct
    62581 ttatttcctt caaagccttt tctgtgtaag aggccgctaa cttcctagct tcttccacgc
    62641 ctcctgtatg ttcaacgatt ttgcgtacgg cgtcgacatc ttcttctgtc attgccgctt
    62701 tcttctctaa ataaggtagt agctttggtt tgtttttttg caaagagtag atcaacggta
    62761 aagaatagac cccttgacgc atgtcttcta gtacaggttt gccgatttcc tggctctctt
    62821 ttgtataatc taaaatatca tcgataatct gaaaggccat cccgatagaa agtccgatat
    62881 ctcctgcttt ttttgcgaag cgctcgcttg ttccactttc aaaagcgcca acagaacaac
    62941 ttaattggaa cagttcagct gtcttacctg aaatattttc caaatattct tttacagaaa
    63001 gttcataatt atagcgatca tccatctgcc ctaattcgcc attcaatact ttttccatac
    63061 tgcgagaatt caattggaga cttttcaagg aagtggagta atcagacaat agtttaaaac
    63121 agcagacaaa taaatagtct cctgcataga cagaaacgac attaccaaac tgactacgga
    63181 tattaggctg accacgtcgc agatccgctt catcaacgat atcatcatgg atcagtgtgg
    63241 ccgtatggag catttcgata gaagctgcca aagcgatggc tttgtctcga tcttgctctg
    63301 gtccaaattg tgagaacaat agctgatagg caggacgcaa cagctttccg ccggcatgga
    63361 tcgtttcttt gatggcttgt tcgacaggtt tgtttttcag gcggacagtt tcttccatcc
    63421 gttttagtgt ttctgtcaat tctttactta aagcaggata gttttcccac attggatgta
    63481 gcttcataaa aaaactcctt gtgtgacgat tattttttgg taaacattgg gctccatttt
    63541 tgtttggttc cctgctcata atctttttgg aatcgttgaa gtgtctgcac atgtcgtaaa
    63601 agataattcg ctgcgatccc gattgcaatc cctgataaaa tccctaaaac tgaaaggact
    63661 ggcaagtaca acatgactgt ccatgattgt gcaatgaaag aagccatcaa aagctgtccg
    63721 acattatgca tgaaaccgcc ggccgcacta attccgatga tactgacacg ttttggtcct
    63781 aattgcttga ccaataacat tccaaaataa ctaagcaaag caccactcat actataaagg
    63841 aatgtggaaa tcgttccgcc tagcaaagta gtcaggaata gtcgtagcca aagtagtaag
    63901 aagctgtctt gttttttcat ggtaaaaatg gcaacgatcg tgattagatt ggctaagcct
    63961 agttttgcac caggcgcaaa agaaaaagga taagggatca tattttccaa taaaccaatg
    64021 atcactcctt gtgcaacaag tagcgaaata taaatcattt tttgtaattt actcatggtg
    64081 tgtgaaggtc taataaatca gactgtcttc gtcacctcca tctgttgaac ggacttcgat
    64141 cactaattta tgtggcaaac agacgatggt ttcgccattt ttcgttgccc agcctcgacg
    64201 tacgcagatc tggtcatcgc aatctgcttc ttttatccgt actcgatcgc catctatctc
    64261 gatcaagtta taatttccgt gttcatcttt gtaagtatat gtgttgctct cttctccagc
    64321 catcaaaggg aatgttttga tttctgtgcc atccacacgc agtaccgctt ctttttctgc
    64381 tgttgcggtg ttttgctgaa gaccaaaaat catgactggt aaaaaagaaa gaacagtcaa
    64441 aagcaaaata ataaaaacat cccaaggttt gagcagactt ttttcagcaa attctttaag
    64501 gctcattttt gttctccttt ttccagatta actcacccaa ttttagcata aaaacaagag
    64561 gttgtgacac ctgtgtcttg ctttgagtaa taagaacgaa gaatagaaaa cagctttcca
    64621 tgttttctaa ttctttgggc ttattaccga gaagcagcag gtggaacacc gtttataaca
    64681 agaggttgtg acagaagtgg gcaacttcaa gaaataagaa ggaatttacg aaaattactt
    64741 ttcaagtttt tgtgaaattt cggcttattt ttcgaagaag ctacttctgg aacaccgttt
    64801 ataacaagag gttgtaacag aggttgtgac acctgtgtct tgctcatgga ggcttatctc
    64861 gggcatctta ttcctttaat tttacataaa aaggaggctg tgacggtttg tctcagcccc
    64921 tttctattga aatcttgcat tcaaataacg ttctttatct atttatttct tgccgtactg
    64981 ggaacttggt ctaccgtacc accaattacc tagtgtacgt tgtagccatg gaattaccca
    65041 gcgatccaac ccaatcgcac gtccggaacc gttcatcaat gcgaaagcaa caaaaatgaa
    65101 ccaaatgttt acccagtaaa acatgcctga taagcagaac gtgatcacca atacaatcgt
    65161 tgtcgcactg catagccatg tgaataatcc agcaatcaat gctaatgcca aggcaatttc
    65221 aaagaatacc atgaattttt ggaagaacat tgctacatca ttgtttggca tgatcacttg
    65281 catgatcttt gtgaaccagt ctggtgactt ctcaatcaca gccattggtt cttcaccgta
    65341 tgcataactc aaaccaaaat gtgcagcttg agtcgctgct tctccggcac ttgcccctgc
    65401 tccgctggca gctgtggttg catcaccagc agcagaagca ccactggcaa catctgttac
    65461 attgtattcc catgtccaag ggaacacact gtttccagtg aaccatgatc cttcaccaaa
    65521 ccaagtactt ggtttgaaca cttcaccatt cccaatgatt tttttggaag cttcaacgaa
    65581 ccaaactaaa ccatagaaaa tacgaagtgg cacactccat aagacattgc cgtaacgaga
    65641 agaatgtcct cgagtcacgt tgcgatcatc tttgatatgg aaaaactcat gcattaaata
    65701 ttggaacata taataaccag aacgaatatc aaagaagtat ttcaaattca cgatatgttt
    65761 cattacgata gctaaaaatc cgcttaaatg gatcttatca aataggttag ctacccccca
    65821 tttggcacca atagaaacca taaatccttg atagtttcct ttaaatttat gcttttctcc
    65881 gcctttgatc gaagccacga tattcgctgc ggctgtgtgt cctgtttgtt cagcagcttg
    65941 aacgatctgc ggtgtcggtg ttcctgggaa ttcttcataa aagactaaat caccaatgat
    66001 atagatgttt ttatcttcgt agccttttgc ctgcatatat tcattggcga tcaaccgctg
    66061 tccgcgtgct gattctaatc caaagtcagc agcgtcagaa gttcctttga caccagctgt
    66121 ccaaatcagt gtgtgagtcg gaatgttgcg tccgtctttc aatttgatat gatcagcagc
    66181 cacttctacg attggtgcat tcaataataa ttccacgttg tgcttacgca aatagcgttc
    66241 agcttttgca gcatcatttc gatccaacat attcaagatc gtcggcattg cctctacgac
    66301 catcagtgtg atttcgtttg gatctaattt ataatctttg gctaggcgat ttttccagtc
    66361 gatcagttcc ccaatcattt caataccggt aaatccagaa ccacagacaa cgaaagtcag
    66421 catggcttta cgcagttcat catctggttc caatgcagct tttgctactg tcttctcaat
    66481 atgttcccga atccttacag aatcttcaaa tgaccaaagg gtgaatccgt gtgctttgac
    66541 accaggtgta ccaaagtcat tcggctctcc gcccattcca aggatcagct gatcaaaagg
    66601 ataggaacct aatttggtct tgacgatttt tcgttcttta tcgataccga ttacagtgtc
    66661 tgtgacaagt tgtacattct tttgacgagc aaacaaatgc tgcagatcgt attggattgc
    66721 ttctggttct acacgtccgc cagctacttc atgcaactcg gtcatcatag tatggtacga
    66781 atgacgatcg atcagagtaa ttgctacatc ttcttttttc agtttctttg ccaagtattt
    66841 tgtagctgaa acaccggcat atcctgctcc tacaacgaca atattttgct ttgccattga
    66901 tcatccgctc cttatagact atctatataa tgaatatatg ataattcttt tagtttgata
    66961 cttattacac aatctccata tattatatag atgttctgtt cttttgtcta actccttttg
    67021 gatttttttc aaattttaaa ataaagcgtt ttttcacgaa atttgctgaa agaaaacgtt
    67081 tgaaatatta catgggttat ttatcaaaaa tagttgactt ataggctaaa aagattagaa
    67141 tgtataatga atcacaaaga aaaattgtgg actagaaatg gaatggtggg gtattaaaat
    67201 gaagaaaaaa cgttttgtaa ctggatttgc cgtattagca ttttcagctc tagtgttagg
    67261 cgcttgcggc gcagatgaca acggaagctc aaactcttct tctgaatcat ctacagcaca
    67321 atcttcgact gcaaaaagca caacagaatc atctgcaaaa gttgtagctg gcggagattt
    67381 gcaagacggc acatataaat tagaagaaaa aaattattca aatggttacc gtgcagtctt
    67441 tgaaatggtt gtaaaagacg gcaaaatcac tgaatcaaaa tatgataacg tcaatgaaaa
    67501 cggcgaatca aaaacaaaag atgcagaata caacaaaaac atggaagcaa aatctggcac
    67561 aaacccagaa aaatacattc ctgagttgaa tgaccaattc ttaaaagcac aaagcgcaag
    67621 cggtgtggaa gtcgtaactg gagctactca ctcatctgaa agcttccaaa actacgcaca
    67681 acaattgatc caagctgctc aagctggtaa cacagataca atcgaaatcg acaatggtgc
    67741 tgatttgaaa gacggcactt acaagcttga agaaaagaac tactctaacg gctaccgtgt
    67801 gcaattcgaa atgacagttg ctggcggcaa agtaactgaa tcaaactttg attatatcga
    67861 caaagacggt aaatcaaaac aagatgatac agaatataat gaaaatatga aagcaaaaag
    67921 cggtacagaa ccaaaaactt acatccctac tctgaacgac gaattcgtca aagcaatggg
    67981 cgaagaagat ggctcacctg ctaatgtaga agttgttaca ggtgctacac acagctcaca
    68041 ttcattcatc atgtacgcac aacaattagt gaatgcagct gaaaaaggcg acactcaaac
    68101 aatcgaagta gataatatcg taacagaaaa ataatcacat aatcatacta ataaaagttg
    68161 gagggtggga cgatagccag attaggcagt cgtctcacct ttcaaaatga agagaggaaa
    68221 aagatgcgga aaaaactact attttcctta atcacactgg gcatctcttt tatggtgcta
    68281 actggctgca gcaactcagc aaataaaaca accactgaaa gttcagcagc taccaagata
    68341 ttgaaagatc catattctga tgaacagttc ttgttaggga cttacgttcg tatccgtgtg
    68401 tatgatgaag gcaaagaatc agcattgaaa cctgccttcg atcgagtaaa agaacttggt
    68461 gataaaatca cgatcaatca aaaaggttct gagatcgatg aagtgaatga gcaagctggg
    68521 atcaaaccag taaaagtttc tgatgacgtc tacacgttag tcaagcgggc ctacgaatat
    68581 agccaagatt ctcaaggcgg attcgatatg gcgattggtg caatcacaca attatggcga
    68641 atcggttttg atgacgccag aaaaccttcc caagaagaaa tcgatcaggc attgaaatta
    68701 gtggattatc ataagatcga actaaatgac aaagaaaaaa ctgtttatct aaaagaaaaa
    68761 ggaatgatca tcgatttagg tgcaatcgcc aaaggttaca ttacggacga agtagtgaaa
    68821 gtcttgaaaa aacaaggagt gaccactgcc atcgttgact tgggcggtaa tgtctatgtc
    68881 ttaggccaca gcccaagagg cgaaaaccaa gactggacag tcggcatcca agatccgaac
    68941 atggctcgcg gatctgtttt aggttctatt aaagaacgga acaaaacatt agttacctct
    69001 ggaatttacg agcgttattt agaagtagat ggcaaaaaat accatcattt gtttgatcca
    69061 aaaacgggtt atccgttcga caatgatatc gcaagtgtca cgatcataac agataaatca
    69121 attgatggtg acgggttgtc tacagcagtc ttttcaatgg gtgtgaaaaa aggactggaa
    69181 tacgtcgaaa gcgaattgaa taacgggacg gaagcgatct tcgtgacaaa agacgataaa
    69241 gtatatgtga ccgatccaat caaagacacc ttcaaattat cggaggactc ccattacacg
    69301 atgggtgacc gttctgaact aaaataagga gtacgaacca tgacactaaa aatcttttta
    69361 gaagtagttg aaatacgaac aaaggtggct agtgtatttc cttttgcggt aggggtatta
    69421 ttctctatcg ctttttttca tgagatacat tggctaaaca cttttttatt ttttattgga
    69481 atgctagttt ttgatttagc gactaccgct atcaataa
//
LOCUS       DAJSAW010000008        62714 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJSAW010000008 DAJSAW010000000
VERSION     DAJSAW010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.008"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ONT64_000600"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ONT64_000600"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR2302038.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ONT64_000601"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ONT64_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR2302039.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ONT64_000602"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ONT64_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR2302040.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ONT64_000603"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ONT64_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR2302041.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ONT64_000604"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ONT64_000604"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR2302042.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ONT64_000605"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ONT64_000605"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR2302043.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ONT64_000606"
     CDS             complement(8053..8523)
                     /locus_tag="ONT64_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCR2302044.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ONT64_000607"
     CDS             complement(8569..8994)
                     /locus_tag="ONT64_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCR2302045.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ONT64_000608"
     CDS             complement(9061..9330)
                     /locus_tag="ONT64_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCR2302046.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ONT64_000609"
     CDS             complement(9341..10936)
                     /locus_tag="ONT64_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR2302047.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ONT64_000610"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ONT64_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR2302048.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="ONT64_000611"
     CDS             complement(14489..15049)
                     /locus_tag="ONT64_000611"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR2302049.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ONT64_000612"
     CDS             15402..16376
                     /locus_tag="ONT64_000612"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCR2302050.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ONT64_000613"
     CDS             16546..17946
                     /locus_tag="ONT64_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HCR2302051.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ONT64_000614"
     CDS             complement(18024..18746)
                     /locus_tag="ONT64_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2302052.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ONT64_000615"
     CDS             complement(18825..19331)
                     /locus_tag="ONT64_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR2302053.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ONT64_000616"
     CDS             19454..21253
                     /locus_tag="ONT64_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HCR2302054.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="ONT64_000617"
     CDS             complement(21321..22895)
                     /locus_tag="ONT64_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR2302055.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ONT64_000618"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ONT64_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR2302056.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ONT64_000619"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ONT64_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR2302057.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="ONT64_000620"
     CDS             complement(24672..25310)
                     /locus_tag="ONT64_000620"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCR2302058.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="ONT64_000621"
     CDS             complement(25311..25961)
                     /locus_tag="ONT64_000621"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR2302059.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ONT64_000622"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ONT64_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCR2302060.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ONT64_000623"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ONT64_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HCR2302061.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="ONT64_000624"
     CDS             complement(29104..29844)
                     /locus_tag="ONT64_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HCR2302062.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ONT64_000625"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ONT64_000625"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR2302063.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="ONT64_000626"
     CDS             complement(31215..32153)
                     /locus_tag="ONT64_000626"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR2302064.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="ONT64_000627"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="ONT64_000627"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR2302065.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ONT64_000628"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ONT64_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR2302066.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="ONT64_000629"
     CDS             complement(35313..35618)
                     /locus_tag="ONT64_000629"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR2302067.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ONT64_000630"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ONT64_000630"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR2302068.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="ONT64_000631"
     CDS             complement(36724..37008)
                     /locus_tag="ONT64_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302069.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="ONT64_000632"
     CDS             complement(37026..37916)
                     /locus_tag="ONT64_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR2302070.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="ONT64_000633"
     CDS             complement(37936..38154)
                     /locus_tag="ONT64_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HCR2302071.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="ONT64_000634"
     CDS             complement(38124..39824)
                     /locus_tag="ONT64_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR2302072.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="ONT64_000635"
     CDS             40091..40864
                     /locus_tag="ONT64_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302073.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="ONT64_000636"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="ONT64_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HCR2302074.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="ONT64_000637"
     CDS             complement(41688..42659)
                     /locus_tag="ONT64_000637"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR2302075.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="ONT64_000638"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="ONT64_000638"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR2302076.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="ONT64_000639"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="ONT64_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCR2302077.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="ONT64_000640"
     CDS             complement(45614..46438)
                     /locus_tag="ONT64_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR2302078.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="ONT64_000641"
     CDS             complement(46411..47091)
                     /locus_tag="ONT64_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR2302079.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="ONT64_000642"
     CDS             complement(47114..48052)
                     /locus_tag="ONT64_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HCR2302080.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48297..49160)
                     /locus_tag="ONT64_000643"
     CDS             complement(48297..49160)
                     /locus_tag="ONT64_000643"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR2302081.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="ONT64_000644"
     CDS             complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="ONT64_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCR2302082.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52016..52822
                     /gene="proC"
                     /locus_tag="ONT64_000645"
     CDS             52016..52822
                     /gene="proC"
                     /locus_tag="ONT64_000645"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR2302083.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52901..54457)
                     /gene="rny"
                     /locus_tag="ONT64_000646"
     CDS             complement(52901..54457)
                     /gene="rny"
                     /locus_tag="ONT64_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCR2302084.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(54868..55917)
                     /gene="recA"
                     /locus_tag="ONT64_000647"
     CDS             complement(54868..55917)
                     /gene="recA"
                     /locus_tag="ONT64_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR2302085.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56026..57267)
                     /locus_tag="ONT64_000648"
     CDS             complement(56026..57267)
                     /locus_tag="ONT64_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HCR2302086.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="ONT64_000649"
     CDS             complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="ONT64_000649"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR2302087.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58294..59217)
                     /locus_tag="ONT64_000650"
     CDS             complement(58294..59217)
                     /locus_tag="ONT64_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2302088.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(59956..61242)
                     /locus_tag="ONT64_000651"
     CDS             complement(59956..61242)
                     /locus_tag="ONT64_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR2302089.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61235..62503)
                     /locus_tag="ONT64_000652"
     CDS             complement(61235..62503)
                     /locus_tag="ONT64_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR2302090.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
BASE COUNT        17307 a        13635 c        11225 g        20547 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48241 aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct tatattttat
    48301 tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt atatactctc
    48361 tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc actcatcaat
    48421 gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac tggatgccgc
    48481 tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg ataatcttgc
    48541 tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg cgtatctact
    48601 tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac aacccagcac
    48661 tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa tgccgtactt
    48721 ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc cagttcttct
    48781 aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc acagtctgta
    48841 cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat cttgacaccg
    48901 gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt gttccgttca
    48961 tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt tatttcttca
    49021 aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc atgataccca
    49081 tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc tttttcaatc
    49141 aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa
    49201 ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca acagctttca
    49261 cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc tgtgagcact
    49321 tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag tagctgcatg
    49381 gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc ccaatgatcg
    49441 acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt accataatta
    49501 tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc aacattagct
    49561 aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac aataaacaga
    49621 ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg caacaaatca
    49681 tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac tttttgatct
    49741 ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg tcccacagag
    49801 aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac agcttgatgt
    49861 aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat caaacgttga
    49921 tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac aatccctgct
    49981 tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta gctttctgat
    50041 atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa ctgattcgtt
    50101 gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca tttgtttcct
    50161 ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata aaaataactg
    50221 taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc caataaacgg
    50281 tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc taaatattga
    50341 taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga gaaccacaca
    50401 tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa aaagcgtccg
    50461 ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct agcatcaaag
    50521 attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct ggataaccac
    50581 acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt acgtgtgatt
    50641 gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt tttgactttt
    50701 actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt cactgtctga
    50761 tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat tgcagacaga
    50821 ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc acttacgagc
    50881 acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt
    50941 ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag aacataataa
    51001 aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga caattgactc
    51061 attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat cgtcagcatt
    51121 cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat atctattctc
    51181 atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc aaaggatgcc
    51241 agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt tctctttaga
    51301 taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca gatccaatag
    51361 cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag aaacgaccat
    51421 acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa aaccattttt
    51481 ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac accaccaaaa
    51541 ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc ttttctttcc
    51601 atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata caacaacatc
    51661 ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg gctttgttgt
    51721 cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc gacttgattt
    51781 gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact atgttccttt
    51841 atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta acttgtatac
    51901 ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt agacctgttc
    51961 tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg ataacatgaa
    52021 agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt tattaaaaaa
    52081 aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa
    52141 atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg catcacaatc
    52201 agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg aagaactaag
    52261 agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat taatagattt
    52321 agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt
    52381 cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg aggaacagct
    52441 tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag cagaatcaga
    52501 tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca tgctgatcga
    52561 tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca ctgagattgc
    52621 tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa atccatggga
    52681 acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga
    52741 agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg agaaagatct
    52801 tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca aaaaaagaag
    52861 agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca taatcagttg
    52921 cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt tcttcttcga
    52981 ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt tcttctggct
    53041 tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct acaccggcga
    53101 agctgttcga aatattttcc agacttctta aacgacggat gtagttttct aatgattcac
    53161 ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg acagagataa
    53221 cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact actgggtttt
    53281 ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca atctcatggt
    53341 caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg atatcttcgc
    53401 caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt
    53461 tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga tctggatgaa
    53521 gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata cgttcatcca
    53581 tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt cgtccatctt
    53641 gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg aatccggaaa
    53701 gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc gtctcaagcg
    53761 ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca ttcggaaggg
    53821 taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga atcgctaatg
    53881 ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca gattctttta
    53941 ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg acatctcgtg
    54001 cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct tcaatcattt
    54061 tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt gcttcttctt
    54121 tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc agcgtttgtt
    54181 cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct ctcagctcac
    54241 tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat gcttcttttt
    54301 ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat gattgtgcac
    54361 cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca agaccgacaa
    54421 ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc atactatctt
    54481 tttcattttt atcatctata cagcttctat gataaattat tctaaactcg tcaatatgca
    54541 tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt gtaccccgta
    54601 gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac gatggttttt
    54661 aaaataaatg acactagttt catataggta aacctttaaa ttgagaaact tttctattaa
    54721 attattcttt gtagatgaac agatattttt attttatgta cccactcgta taattaaata
    54781 actcacgatt tctttattca ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag
    54841 accagccgtt tttgtttatc ataattttta ttcttctact aaaggcaatt cttcttgtcc
    54901 tttttcatct tctgggacag cgacttcttc accaatacca taagctgctc taactagagc
    54961 agaaacttct gccatcattt caggatgatt agccatatag attttagcgt tttcccgtcc
    55021 ttgaccaatc cgatcttctt tataagaata ccaagcaccg cttttatcaa caatgtcttt
    55081 ttctactgcc atgtctagaa gttctccttc ttgtgagatc cctaaaccat acatgacatc
    55141 tacttcagcg attttgaaag gaggtgctac tttgtttttt acgactttga tctttgtacg
    55201 gttaccaaca atatctgtac cttgtttcaa ttgttctgca cgacgtactt ccaaacggat
    55261 cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt tctggattcc caaacatcac
    55321 gcctactttt tcacggattt ggttgatgaa aatagcgatt gtttttgttt tgttgatcga
    55381 accagagagt ttacgcaatg cttgagacat caaacgtgct tgtaacccga catgtgagtc
    55441 acccatttcg ccgtcgattt ctgctcgtgg aactaaagca gcaactgaat cgactactac
    55501 gatatctaca gccccacttg atactaaagc atcagcgatc tctagacctt gttctcctgt
    55561 gtcaggctgt gaaagaagta gttcatcgat attcacacct aatttttgtg catattgcgg
    55621 atctaacgca tgctcagcat caatgaaagc ggccgttccg ccattttttt gtacttctgc
    55681 aatagcgtgt agtgcaactg ttgttttacc tgaactttca ggtccatata cttcgatgat
    55741 acgtccacgg gggtaaccgc cgacacctaa agcgacatct aacgcaagag agccacttgg
    55801 aattgtagag atttgttggt cgattttttc gcccaatttc ataatagagc ctttaccata
    55861 gctcttttca atttttttca gtgcagcatc taaggctgct ttacgatcat ctgccaatac
    55921 gataatcctc cttatattta caaatcagag caacatttca ttatccttat catatctttt
    55981 ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc atttatcatt ttcttaaaag
    56041 ctgttttctc aaaagatcaa atccttgcat aaccgcatta tatctgatag cagcacgatc
    56101 tctggcaaag tgatattttt ccgaaaccac gccgtcttta gatgaaatag cgatccatac
    56161 tgttcctgca gattgacctt ctaacgtgtc cggtccagcc actccggtga aagctacact
    56221 gtaatctgtt cttgctatct cacgtgcttg aatggccatc tgctccacac attctttgct
    56281 gactgttcca tattctgcta atagctccgg atcaatctcc agaaaaccag cttttgtttg
    56341 gagcgagtag gtaacgaatc cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa
    56401 tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct gttacagtct gcttgttttc
    56461 tttcagtagt tccactacga cttttgctag cgaattatca tcgccgtatc cataaaaata
    56521 ttcccctact cgttcttgga ttttttcttc taatgctaaa agagcttgat tccctgcctg
    56581 aacatcattt gttttcactg tcagtcgtaa tgtcacttca tttggtttgg catatggtgc
    56641 gatcgttgga ttgatctgtg tctcaatcag atcttttagc tcagtgacca gtcgcgattc
    56701 accaatccca taaaagcgca aaactttaga aattagtttt tcttcagaag gaaaacgttc
    56761 ctctagtaaa ggacgtactt gttccacaaa cattggtttc aactcattag gcggaccggg
    56821 aagaaggata tacgcatgtg tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg
    56881 atttggtaga ggaaccccgc cttcgataat ctgggactgt tgcagattgt ttttggtcat
    56941 ttttcgatgg gttgtttcaa aatatgccaa taattttttg tatccttccg tattttgtat
    57001 gagagactta ccgaggtgag ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc
    57061 taatccgcca cataagagta tgagttcact tcttgtttca gctagagcaa tcaactcttc
    57121 taaacgctgc tgattatctc ctacaactgt ttgataataa acttcgatac ctagatcagc
    57181 tagctgttct gacaagaaag tggcattcgt attcacgact tgtcctagta aaagttctgt
    57241 tccgacagct attatttctg ctttcattac ttcccgctcc cttatatagt atacgctgta
    57301 tttttatatt tttttattta aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc
    57361 tccttgttcc aaataaacag tgttctcctg ccaaaacctc ggtcataagt cacaatatac
    57421 ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg actcagagtt agacggcagg
    57481 ctcacaacct ctccactaaa cgatacttac attgatcctt tgaacacacc gctgtttttg
    57541 atgaagtaat ccacacctga atagatagta aagatcagac aagcataaag catgatttga
    57601 tctactggaa taccgatcag gctgaatggg atattattga taaacagcaa gataatagca
    57661 accatttgtg tagccgtctt gacttttccc ggccaagctg cagccatgac ttctccatgt
    57721 tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac gacagacaat gatcgctact
    57781 acccaaccag gggcctttcc ttggtcaacc aacatgataa aagcagtcat aactagcatt
    57841 ttatctgcca acgggtccgc aaacttgcca aaattagtga caaggcctcg cgcacgggca
    57901 atttttccat ctaaccagtc tgtgatacta gctaaagcaa aaataactgc acctactagc
    57961 tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc cccaatctaa aggtaaacct
    58021 acaaccaaaa tgaaaatcgg gatcatacaa attctaagta cagttaattt atttggtaaa
    58081 ttcacactat cgactccttt cttccctatt ctacgtgact atcagcgaag tttccaccga
    58141 tattatttac ttttccaaat gaatttattc aaaggagtat ttttattgaa attgatcttt
    58201 ttgaattaac gaagaaggac cgcgacacag atttgtcacg atccttcttt tttataccta
    58261 caagtaaaca gatgcgtaaa ctaatttata cgatcattct gcgtattgaa gagtaaggtt
    58321 cacatttttt ggactctgat tgttttcccc tggattaact ggtacggatt ctccattcgc
    58381 tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg
    58441 taacacggta ctctgtgttt catttgctgc cagtgtccct tgataaacat aggcattgtc
    58501 tactagcaca cctaaccata cacgattgac agcagtaaat tcaagggtta caggcttggt
    58561 agcgtctgtg atatttactg cgatggcact gccagaatct tctcctgtag ctattgccat
    58621 tttcttttct tcacttgatt ttgttgttga ttctactgta gaagactctt tggtctgcgt
    58681 gctttgctct gaggaagagg actcctcgac tgatccttct actttaagcg aagaagctgt
    58741 gtctccgatt attggatcag cttgacgatc ttgccaagtc atatagccca cgacaacgac
    58801 aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga cttgtccaaa agcgacttgg
    58861 atgcgattct tcctgatgca ttgcttttct agaagtactt actgtttcag gctgcggacg
    58921 ttcagggacc gatcctgtca atttgctttt cccatcaaaa acatcgacta aatggtctcc
    58981 atcttctcct acagcttcag cgtattgccg aataaatgcc cgcacgtaaa atttaccagg
    59041 caattggtca aaatcatcat tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat
    59101 ctgctgtaat tcatctaaag atattttttt attcaggcgt gcttgccgta atttttctcc
    59161 gatggtttca aaagccactc gttcatcttc tccagtctgt ttgattctcc gaagcatctg
    59221 ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa aagtcatgtc acagctcctt
    59281 ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc acaagtcaca atatccaata
    59341 aatataagaa gctgtttttt gaacttgttc cttgtgactc ggagttaaac ggcaggctca
    59401 cgacctcttt cttaaacggt gttcaatcag ctgttcctcg gtattagctc aaaaaccggc
    59461 aaaatataag gagatatttt tccgtatgtt tctctaatac tcaggtcaaa acgctgattt
    59521 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
    59581 gttaaaaaca ttaagaagct gtttttactt ggctgttctt attacttgat gcagggcagc
    59641 tttttcacaa cctcttggta cacggtgttc cagaagtagc ttcttcggca ataagccgaa
    59701 atatcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    59761 acttctgtca caacctcttt gattacttcg tttgatttta ttttaatata agttgattaa
    59821 aagagagcat gatttgtttt attttctcac taatttttcc aacacaacta agaataccat
    59881 aaaagctgat tttggaggag gttcctacta caagtctaaa acgaaattta agacttttcc
    59941 tcataaaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct cttcacttat
    60001 aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga tttcaggaag
    60061 atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa tatattcgat
    60121 cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat cgagatttgt
    60181 ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt tttctgctgc
    60241 tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac gatccaagct
    60301 gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta gatagtttcg
    60361 agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat agcgtaacag
    60421 ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa atttatctcg
    60481 tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt cagggaaata
    60541 acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc gaataagttt
    60601 catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc ttggctgata
    60661 aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt
    60721 gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta tgccaaaaaa
    60781 catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga ttcctttttc
    60841 cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag
    60901 attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa
    60961 agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat cttctttttc
    61021 aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct tttctttttc
    61081 accataggga ataaattcgt tatcgatcga accataattc gtactgaaca aaccatatgt
    61141 cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca gtacttcatg
    61201 ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt cctcctgcta
    61261 aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct ttgatcgtta
    61321 cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc agccagctgt
    61381 ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga tattggtttc
    61441 tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt cgcaaacttt
    61501 ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg ataccggttt
    61561 ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa gctagacttt
    61621 ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat aaaccgttaa
    61681 agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga taagctaggt
    61741 tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata ttcacttgag
    61801 gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt aattgcccaa
    61861 ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg atttgatcat
    61921 ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg tccaaatctt
    61981 cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa agttcttgca
    62041 aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat agacgcagat
    62101 tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata tttggataaa
    62161 ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca ttgacatatt
    62221 caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt tttctaactg
    62281 ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt gtctgatccg
    62341 aataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc gtgatgactt
    62401 cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat tgttttgttg
    62461 gtaagacatg aagattcacg ccttctgcca attgatacac catatttata tgctcctttt
    62521 cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta cttattcctc
    62581 tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt tacactttat
    62641 agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta tattgttttt
    62701 ttatttttta tttt
//
LOCUS       DAJSAW010000009        62323 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJSAW010000009 DAJSAW010000000
VERSION     DAJSAW010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.009"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            591..1280
                     /locus_tag="ONT64_000653"
     CDS             591..1280
                     /locus_tag="ONT64_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2302091.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ONT64_000654"
     CDS             1267..2475
                     /locus_tag="ONT64_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2302092.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ONT64_000655"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ONT64_000655"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR2302093.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ONT64_000656"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ONT64_000656"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR2302094.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ONT64_000657"
     CDS             complement(6578..7273)
                     /locus_tag="ONT64_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HCR2302095.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ONT64_000658"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ONT64_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR2302096.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ONT64_000659"
     CDS             complement(8472..8663)
                     /locus_tag="ONT64_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCR2302097.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ONT64_000660"
     CDS             complement(8684..9574)
                     /locus_tag="ONT64_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR2302098.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ONT64_000661"
     CDS             complement(9561..10328)
                     /locus_tag="ONT64_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR2302099.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ONT64_000662"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ONT64_000662"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR2302100.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ONT64_000663"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ONT64_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR2302101.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ONT64_000664"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ONT64_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR2302102.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ONT64_000665"
     CDS             14899..15282
                     /locus_tag="ONT64_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302103.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="ONT64_000666"
     CDS             complement(15450..16208)
                     /locus_tag="ONT64_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HCR2302104.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ONT64_000667"
     CDS             complement(16226..17044)
                     /locus_tag="ONT64_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR2302105.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ONT64_000668"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ONT64_000668"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR2302106.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ONT64_000669"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ONT64_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR2302107.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ONT64_000670"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ONT64_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR2302108.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="ONT64_000671"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="ONT64_000671"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR2302109.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="ONT64_000672"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="ONT64_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCR2302110.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="ONT64_000673"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="ONT64_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR2302111.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="ONT64_000674"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="ONT64_000674"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR2302112.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="ONT64_000675"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="ONT64_000675"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCR2302113.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="ONT64_000676"
     CDS             27086..27385
                     /locus_tag="ONT64_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR2302114.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="ONT64_000677"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="ONT64_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR2302115.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /locus_tag="ONT64_000678"
     CDS             27973..28209
                     /locus_tag="ONT64_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR2302116.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="ONT64_000679"
     CDS             28352..30325
                     /locus_tag="ONT64_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HCR2302117.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="ONT64_000680"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="ONT64_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR2302118.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="ONT64_000681"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="ONT64_000681"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR2302119.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="ONT64_000682"
     CDS             33204..34496
                     /locus_tag="ONT64_000682"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR2302120.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35232..36095
                     /locus_tag="ONT64_000683"
     CDS             35232..36095
                     /locus_tag="ONT64_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR2302121.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36224..37846)
                     /locus_tag="ONT64_000684"
     CDS             complement(36224..37846)
                     /locus_tag="ONT64_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2302122.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38095..38616
                     /locus_tag="ONT64_000685"
     CDS             38095..38616
                     /locus_tag="ONT64_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCR2302123.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="ONT64_000686"
     CDS             complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="ONT64_000686"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCR2302124.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40697..41515
                     /locus_tag="ONT64_000687"
     CDS             40697..41515
                     /locus_tag="ONT64_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HCR2302125.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41618..41815)
                     /locus_tag="ONT64_000688"
     CDS             complement(41618..41815)
                     /locus_tag="ONT64_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302126.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41984..42562
                     /locus_tag="ONT64_000689"
     CDS             41984..42562
                     /locus_tag="ONT64_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCR2302127.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            42657..42794
                     /locus_tag="ONT64_000690"
     CDS             42657..42794
                     /locus_tag="ONT64_000690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302128.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(43607..44110)
                     /locus_tag="ONT64_000691"
     CDS             complement(43607..44110)
                     /locus_tag="ONT64_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR2302129.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44469..45494
                     /locus_tag="ONT64_000692"
     CDS             44469..45494
                     /locus_tag="ONT64_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR2302130.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45817..46695
                     /locus_tag="ONT64_000693"
     CDS             45817..46695
                     /locus_tag="ONT64_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HCR2302131.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47171..47287
                     /locus_tag="ONT64_000694"
     CDS             47171..47287
                     /locus_tag="ONT64_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCR2302132.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48215..48553
                     /locus_tag="ONT64_000695"
     CDS             48215..48553
                     /locus_tag="ONT64_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302133.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48647..48877
                     /locus_tag="ONT64_000696"
     CDS             48647..48877
                     /locus_tag="ONT64_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302134.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49277..49648
                     /locus_tag="ONT64_000697"
     CDS             49277..49648
                     /locus_tag="ONT64_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCR2302135.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(49922..50614)
                     /locus_tag="ONT64_000698"
     CDS             complement(49922..50614)
                     /locus_tag="ONT64_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR2302136.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(50617..51402)
                     /locus_tag="ONT64_000699"
     CDS             complement(50617..51402)
                     /locus_tag="ONT64_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR2302137.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            51639..53039
                     /gene="lacG"
                     /locus_tag="ONT64_000700"
     CDS             51639..53039
                     /gene="lacG"
                     /locus_tag="ONT64_000700"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCR2302138.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            53064..53381
                     /locus_tag="ONT64_000701"
     CDS             53064..53381
                     /locus_tag="ONT64_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR2302139.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            53394..55067
                     /locus_tag="ONT64_000702"
     CDS             53394..55067
                     /locus_tag="ONT64_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HCR2302140.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            55081..56712
                     /locus_tag="ONT64_000703"
     CDS             55081..56712
                     /locus_tag="ONT64_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HCR2302141.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            57584..58774
                     /locus_tag="ONT64_000704"
     CDS             57584..58774
                     /locus_tag="ONT64_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HCR2302142.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            58908..61097
                     /locus_tag="ONT64_000705"
     CDS             58908..61097
                     /locus_tag="ONT64_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2302143.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            61094..>62323
                     /locus_tag="ONT64_000706"
     CDS             61094..>62323
                     /locus_tag="ONT64_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR2302144.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILI"
BASE COUNT        19425 a        11323 c        12309 g        19266 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaacaaa cggtgttcaa aagcttgcat ctacggcact aagcccaatc
    34681 agatgaaaaa tatggagtgc tatttttatc tgattgttct tactgcttga tgcagagcag
    34741 ctttttcaca acctctccac taaacggtgt tctcctgcca actcctcggt cacaagtcac
    34801 aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact cggagctaaa
    34861 cggcaggctc acaacctctt ggtaaacggt gttcaatcag ctgaccctcg gtattagctc
    34921 aaaaaacggc aaaatatgag gaactatttt ttccgttttt ttctctaata ctcaggtcaa
    34981 aacgctgatt tcacaacctc tgattcacgg tgttccagaa gtagcttcta cggaaataag
    35041 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    35101 tgaccacttc tgtcacaacc tcttattttg attagaactt gcgaaaataa accggttaaa
    35161 gtgtgtctag cgcaagagat ttgctataat agacgataag ggtaaaatgt aacgagaagg
    35221 ggttgaaaaa tgtgtatcaa attgtaacag attcttgttg tgatctgcca taccatgtat
    35281 tagcaaaaga aaaagttgat tttttatcaa tgaatctcca attgaatgat caagagttag
    35341 tagatgacct cggaaagaca ttcaattatg accgctacat gaatcagctg aaaaatggtg
    35401 cgatgcctac gacatcacaa atcaatgttg gccgatattt agaatttttc cgttcttatg
    35461 cggaaaaaca gattcctgtt ttatatgtgg ctttttccag tggattaagc ggctcttaca
    35521 gtagtgcgct tcaagcggtt gaattgctga aggaagaata tgaagaccct gagatctatg
    35581 tttttgatac aaaagctgca agtattgggg aagggctttt agtattggaa gctgtaagat
    35641 tgaaaaaaga aggtaaaagc atcgaagaaa ccctagagtg gctggaaagt aattatttga
    35701 aagtccattc atgggtaaca gtagacgatc tgaaatatct agaacgaggc ggtaggattt
    35761 ccaaagcaag tgctactctt ggcagtctat tgagtgtaaa gcctattatt acggtggata
    35821 aagaaggaag gctgacgaat gttgacaaag ttcgaggaag aaacaagtcg atccagacaa
    35881 tcgtcaatga gacggtaaaa acaatcgttg caccacttga tcaaatcgtt tatgtggctt
    35941 acgctgggga tctagagagc gcggagaagg caaaacggct tttggaagag cagataaaga
    36001 tgaaagatgt gaagctttat cccttaggac caacgatcgc cagtcacaca ggctatggct
    36061 gtatcgcgat tttttcgatg ggaatcagtc gataaaagca gtaactcatt ctgtaaaaaa
    36121 gcccagaaat tgattgtaac aaaaacagcg actcttaaaa gatagatgag catcctatct
    36181 cttagaggtc gctgtttttt tattttttaa gctaaatagg ccattatgcc cataactcgt
    36241 tcaattgttt ttgaatatct gcattttcca aaaattcatc atatgtcgta tcagctcgat
    36301 cgatcacacc tttatccgaa acggcaatga tgcggtttgc caatgtttgg ataaattgat
    36361 ggtcatgtga cgcaaataag attgagccag taaatgccat caagccgtca ttcaatgctg
    36421 tgattgattc taagtctaaa tggttcgttg gatcatctaa tacaagtacg ttagctttcg
    36481 aaagcatcat ttttgaaagc atgcaacgga ctttctctcc accagataaa acattcactg
    36541 gtttcaatac ttcttcacca gaaaacaaca ttcttcctaa gaagcttctt aagaaggtgt
    36601 tatcatcttc ttctttactt gcatattgac gcaaccagtc taaaatcgtt agatcagtat
    36661 cgaattcttt cgttgtatcc tttggtaaat aagaattact tgttgtcaca ccccaacgta
    36721 ctgttccagt atctggcgta atttcaccca tgataacttt gaatagcgtt gttgttgtta
    36781 tatcattttc tgcgacgaaa gcgaccttgt cttctttgct caaattaaaa gatatatcat
    36841 ctaaaatttt ctttccatca atcgttacac ttacattttc tacctgcaat aagtcgtttc
    36901 caatctcacg ttcaggtttg aatccaacga atggataacg tctagaagaa ggttggatat
    36961 cgtctaaagt aattttttca agcatttttt tacgtgaagt tgcttgtttt gattttgatg
    37021 cattcgcact aaagcgggca ataaattctt ggagttcttt gatttgttct tcttttttgg
    37081 catttgcgtt tgcttgaagt ttagccgcta gttgactaga ttccaaccaa aaatcataat
    37141 ttcctacata aagtttgatt ttaccaaaat ctagatccgc catatgtgta catactttgt
    37201 tcaaaaagtg acggtcatgg gatacaacga tcacagtatt ttcaaaattg atcaaaaatt
    37261 cttccagcca attgatcgat tggatatcca gaccgtttgt tggttcgtct agcaaaagaa
    37321 catcaggttt accaaatagt gtttgtgcta ataacacttt taccttttgt ccaccagtta
    37381 attcactcat ttgctgatgg tgcaattctt cagggatgtt cagaccttgc agtaaaacag
    37441 ctgcttctgg ttcagcttcc catccattaa gctcagcaaa ttctccttct aattcagctg
    37501 ctcgaatacc atcttcgtca gtgaaatcct ctttcatgta aatcgcatct ttttctttca
    37561 gcacttcata cagacgcttg tgtcccatga tgactgtttc aagtacagta tagtcttcat
    37621 agtcgtaatg gttctgtttc aatacggcca tccgttcatc tggtcccata gtaacggaac
    37681 ctgtggaagg ctgcagatcc cctgataaca ctttaaggaa cgttgattta cctgcaccat
    37741 ttgcaccaat caagccatag cagtttccgg gggtaaattt aatattcaca tcatcaaata
    37801 gtttgcggtc tgaaaagtgc aaactcacat catttacagt aatcaatttt tttcctcact
    37861 tttatcatta ttagttttga caagtcttat agaattatat aggggaaagg taaaagattc
    37921 aaggcaatcg aacattcttt gtgtttcacg tgaaacagaa tgaaaaaaat aaaaataaaa
    37981 gcggagcgaa aatagctact ataggattta ttagtttgca acagcataat ttttgacgtt
    38041 atttataaga gggagtacta taaaactgta aaagaaatga aagaaggatt gaaaatggat
    38101 gaaaaaagaa ccatcgactg gggctcattg attttgggaa ttttatttgt acttgtctca
    38161 ttgttatcat ttcaagaccc tgtcggaaat ttagttgcaa ttgtagtagt ctttgcgatt
    38221 tttgccttta ttaaagggat atttgaactg tttgtacgga acagattaaa agagctaaca
    38281 ggatataaag gaaagatgcc cttaataatc gggatcatcg atattctagt tggcgtcttt
    38341 ttcttattca atattggtgc aggagtcgca gcattgcctt ttgtttttgc agtatggttt
    38401 attgctgatt cagttttagc gttatttact gctgacttgg caagaggagt aagtgaagga
    38461 tattattggt ttacgatcat cgtcaatatt ttaggaattt tattaggaat tttcttattg
    38521 tttaatccaa tttcttctgc gttgacatta agctttttag ttggattcta ttttatgtta
    38581 tttggtatca cacatatcgt ctatgcattt agataagtaa aatcaaacaa ataaaaaagc
    38641 cggcttccga ttacagtttt actgttgtaa ttggaagccg gcttttttat catggacaaa
    38701 tatgcacgag gtgattttta ggtgtcgaga caaatttatg caaaaatgtg agaagctata
    38761 tacacaaatt ttatcttaat actcgaagca tagcgcagtt gtcacaacct cttttcatat
    38821 aattcattca atactggcaa atcatggact tcatattttt ctttgaatgc ttggatttct
    38881 tcttcagaat attttcttgg atctaaatgc agatcttcta ctggcaatgg gcgcttgtta
    38941 ttgatcttca catcgattac tacaggacga tctgattttg cagctgcatc aaaggcaaat
    39001 cgaagttgcg agtattctgt gactgtaaag ctatttgccc cgagcgcttc tccaacttta
    39061 ccgaaatctg catcttctaa aaatacacca aatttctttt gttccgtatc ttcttgttct
    39121 gcttcgatga aaccaaaaga atcattcgaa aagacaacat taataattgg aagctgatat
    39181 ttgacttgtg taatgatatc ttgcatgacc atagcaaacc cgccatcacc acttaaggta
    39241 aatacttgtt tttctgggta gcttagttgt gcggcaattc ctcctggcac accattaccc
    39301 atagttgcga accatccaga tgtcgtatgt ttttgtttac cgttcatatt caataaacga
    39361 atagaatgga tagtcgtgtt accgacatct gtgacgaaaa tcgcatcgtc ttcagcaata
    39421 cgattgattt ctttatagac agcctctgga cgaatcggtt cttcctctcg atcttcaaaa
    39481 gatttcaacc aatttaccca tttttcttta ttttcttgat ttgctaggta ccagctatca
    39541 gcagggcgag catcccctaa ctcgacaagt ctttgcaaag ctgtgtttgc atcaccgagg
    39601 atcgacaaag atgtttcatg gcgtcgtcca aattttgacg catcgatatc gatttggata
    39661 aattcagcag ttggattaaa aaatgcacgt ccaaatggga agtcgctacc gacaaataag
    39721 atcagatcgg cttctgccaa tgcttcattt ccgggtttag tagcgacacg tccagcaaaa
    39781 ccaagaaagt tttcataatc atcaggaata attccttttg ctagaacgga agcgacaaca
    39841 ggaactgaaa aatgttctga gaattctttg atttgcggaa atccgtttcg cgttccttga
    39901 ccaacataaa gaactggttt ttttgctttt tcaagataag gcaaagcagc tagcaaatct
    39961 ttttcttctg gtaatatcac acttgttttg tgagtgtgag cggtggagaa aggttgttcg
    40021 tcaatttctt caaaaccaag atcaacaggg atagtcacga tagccacgcc tttatgctca
    40081 taggcggctt taatggcttc atctactaca tgcggaagac tttgtggtgt catgactgta
    40141 cgattgtata cgctaacatc tgcgaaaata ggattttcat ttagttcctg gaaagagtta
    40201 tagttcatgg atgtagaagc aacttgtcct aagaacgcta ctagaggcac atggtccatc
    40261 tgtgcatcat acaaaccatt gatcaaatgt gtcgcaccag gtcctgcaga gccgaagaca
    40321 gcgccgactt tccccgttaa ttttgcatct gcagcggctg cgagtgcgcc gacttcttca
    40381 tgacgaactt gaatgtagtt gatttcggtt ttttcttgat atagcgcatc cataattgaa
    40441 ttgaaagatc ctccgggaat gccgtaaata tggtcgatac cccagctttc aagaactttt
    40501 accattgcga ttcctgcatt gatttttgac atggtgtttc actcctttag caatgatgat
    40561 acataaattt tttgtatcta gagtatagaa ctttttatcg aaaatcccaa ctaattcgtc
    40621 ttttcacaaa aatgtagtca tgtttttggg aatatgctag agtagtacat agatagattc
    40681 aagagagggg aatggcatgg ttgaatggct attgagactt gaaccttggc agcaagcact
    40741 tgctgggaca gcttttactt attttatgac agctcttggt gccgggttgg tttttttctt
    40801 taaagaaatc aaaaaagaag tgctaaactt gatgttagga tttgcttctg gtgtcatgat
    40861 tgctgcaagt ttttggtctt tgttagatcc ggctattaca aaagcagaag aaaatggaga
    40921 tattgcttgg ctagttgtaa gtattgggtt tggtttaggc ggtgtgtttt tatatatggc
    40981 agataaaaca ctgccccata tgcattttgg accacaacat gaagcagaag gattgcctac
    41041 ccatttaaaa cggacgatct tgcttgtttt ttctattaca ttgcataaca ttcctgaagg
    41101 tttagccgtg ggggtagcat tcggtgcagc agcaactgcg gataatccga cagccgctat
    41161 attagccgct gtttcggtgg cgctaggtat tggtatccaa aacttcccag aaggagcagc
    41221 tgtatcaatc ccgttgcgac aagaaggtct gagtcgtaaa aaggcctttg tttacggaca
    41281 agcttctgga atcgttgaac caattgctgg agtgattgga gcgctactag taacgaaagt
    41341 ggagctgctg cttccttatg cacttgcttt tgccgcagga gcaatgattt atgtcgtggt
    41401 ggaagaattg atccctgaag cacagcagac attgtccagc aaacgtcatt ttgcagtttt
    41461 tggcgtcatg agtggattta tcattatgat gattttggat gtggcattag gataaataaa
    41521 aaaaatatat atgatatatc aaatagggcc gcgaacttat atcgcgaccc tattttttta
    41581 gtaagttata tcataatgaa gtgaatggta tcatacatta ataatagttt tttctatgtg
    41641 tatttccttt gatccagcga acaagccaga tgataagtgc gattgggatt agaactttga
    41701 ccgcaaacca taaaagcccg cttaggacac tgacaaaaag actgatgacg aagctagctg
    41761 caattagcac taaaagaacc atgaagatat ttaagaacat atttttttga cgcatacaaa
    41821 accctcctct tgttcattct ttactataga aaaagtataa cagggattgt ttaaaaacac
    41881 ctccaactga agactgattt ttacttcatc tacttaaatg atcaaacaat tgctttttct
    41941 ctggtttatc atctaaaata gagcggaaaa ggaggagaat atcatgccta aaaatcgtct
    42001 tgaagcattt acagatgctg taatcgcgat tatcatgact attttagttt tagaacttca
    42061 tgaacctaac ggagacacgt gggaggcgct cgctgcatta ggttaccgat tttttatcta
    42121 tattgtcagt tttatcattt tagcgattta ttggaacaat catcatcatt tatttcaagt
    42181 tgtacataag ataaatggac gggtcttatg ggcgaacagt ttctttgttt ttgcgctttc
    42241 gctttttccg tttgcaactt catggatcag tgagtacatc aatagcctgg ctccagaaat
    42301 gacttatggt tttgttacct tgaccgctaa tattgcgtat tatatcttgg caaaagaact
    42361 tgtcaaagca aataaaggcc acgagaaagc attgaaggcg atgttgaata attacaagaa
    42421 atcgtatcta tcgattggtt tgaatgtgtt aggaattgtc ttagggtatt tagtagcgcc
    42481 agtagccgtg ctgatcgcaa acatcgtgat tctagctgct tggatcatcc caagtcgtac
    42541 catcgaatca caatatcggt aaaatgatcc gtgtttaaaa tttaagagaa aaatatagaa
    42601 aaaataaata aacgatcaaa tatccgagtg atactagtat tcaaagaggg atgcagatgg
    42661 atcaaaaaag aattcatgta ggactgatta ctagttttgt cacgatgctt atttatttgg
    42721 atcaaggtca tttgacttgg acagtagttg gtgtcgtagg atttttgaca ttattcacag
    42781 caataaaaaa ataaacatct ttatgtaatc tgatgttcta tctatttaag aaaagcatgt
    42841 ttacagcagt atgattctgt tgtaaacgtg tttttatttg tggaaaattt aatttttaga
    42901 aaagtgtatc gaaaaataga tagttagttt ttccacagtg gataacttcg taagagataa
    42961 aaataaaaaa ttagtcaaag aagggcgtat agatttcttt tgataggaga tatacaacag
    43021 aaatgaataa atattaacgt gttttcaaac agtataaaac ggtgtggttt ttggaataaa
    43081 aaaataacat atctcatttt acgcttttgt tattttatac ttaaaaaata acgacacaga
    43141 aaataccgtg tctcagattt ctctactact tctaaaaact actgtttttt tcctttgaaa
    43201 tgccttcaaa aagtaggatc aggcagaata tactggcaga atcaaggaga agggagccta
    43261 aataaggaga aaaaccatag aaaatataaa gtaattactt ataaaacaag ctttttatag
    43321 aaaaataata tacctgagta gggtataaat taagacggaa ttcctttcta gattgagata
    43381 ttgactagga agaatgttaa gattttaagc ctttaagcta aaaaaaaacc ctttttgtta
    43441 aataagggga atatagcctg tcagtttcaa actgcaagtc gtcctgctgc aataatatag
    43501 tagattagag atgaaaatag atttttttaa accaatgact gaataaaaag accgtcatag
    43561 atacacattg gaatgtgtct attacggtct ttttttacga acgattttat tgaagacgga
    43621 cagttcgggt caaaaataaa aaagctacga tcagaaataa gaataaaatg tatcccaaaa
    43681 taatggctag acggtctaac ttatttgtcg atagatgttt gtttagaagt tgataggccc
    43741 ataaacctat cagtcctcct gttgtattag ttatcaaatc agtgatgtcc gctacgccaa
    43801 taccgaatag gaattgaatc aattctgcca agaagcttac aaggaaaata atccctaatt
    43861 ttttagaaaa agaaagtctc ttgaaattga cattcaaaag caccccaaaa ggaaaaaaga
    43921 caatcaagtt ataagtggtc tcacgtaata ccgtcgtatt ttcaaaagaa aaagtagaaa
    43981 aagggacgag attgacattg ctgtaagaat aagtgagaaa ttgtggaaga ttaaatgata
    44041 gcttgaataa aatcaaccat aatagaaaga ataaataaat ccctaataaa aattttgata
    44101 cataccgcat agacatctcc tccatttaaa aagtatacca tcgaaaaccg ctgaaaaatc
    44161 ataaaaaagt atgaagttta aggaccaacg tagttatcgt acgaaaatta gaagcttttt
    44221 tttgttggga atccggtttc tttaacaaag ctaagatagc gagttgcttg gaaaagttgc
    44281 tatctttttt ctgtcaaaac gactcggaat gttcgttttt tgttctaatt tctccaagtt
    44341 gtttactttt tgatactaag tttatgaaaa atcaagaaat agttgttata ctaggtaatg
    44401 ctgacaaacg ggattggcat ttcatcttgg ttcagctttt tttccaatta ctaggaagga
    44461 gggatagatt gttcaagact tattttcgtt tcaataaaat acaagataat ccttttcgta
    44521 tcatgaatct gatgctactt atctttattg tattatggtt aggttacata aatcatgatt
    44581 ttttgatttc aagtttttca tcattaggga tctttacttt tttctattat caaaacatac
    44641 cgatgaaata tttgctgaaa cgtatgtttc tgatcggttg cggactgttg gttgctttcg
    44701 ttttggggat cttatcgact tatgttttat ggcttgaacc atttgccgta gctttggttg
    44761 ctttttccag cagattcgtg ttgcgattat tccatatttc taaaccaggt ggcttgtttt
    44821 ttgcgatgct ttctgccatg ggaacgagca tgcagctgcc aatcgctcaa ttaccgatcg
    44881 tctcacttta tttttttatg ggtgtcgttt ttgccctgat tgcggcagtg attacaaaat
    44941 tactggattc acggcctgag cagacaattg aaaaagcaac tttaaaagaa agatttcacg
    45001 aagagccttt agtgatcatt gattctgttt tctacagcgc tgccttattt ctttccgttt
    45061 atgtcagtca cggcttgaat ctgcataatc cttattggtt gaccttgtca tgtgcatcga
    45121 ttttgctagc agaaaacttg gatgcgatga agcatcgaca agtccaatat ctgattggat
    45181 cgatgggtgg gttatgtgtg tcggcattct tgtcatttgt gccgtttacg cagttgcaga
    45241 ccatttttct gatcactttt ctatatggaa tcgcacaatt tctagttgct agaaattatg
    45301 cagttgctaa tatttttctt aatccgatgg cgttgatgct atctacactt attcgtggag
    45361 catacttgat ttctttgatc gaatatcgtt ttttagggat cgtcatagga agtttcatcg
    45421 gtttaggtgt tgcgtgggta atgacggttg gtttgcaaca ttatttaacg gttgttcgtc
    45481 aaaaattaga atagaaggac aaatatatgt gccaaaagag gttatgacaa gatttttgtc
    45541 acaacctcta attcaaggtg ttcaaaagcc agattctcag cattaagtca aattcacaca
    45601 aaaatgtgga aaggaacttt caaaaaaatt ttatatttat tctatcaaaa aattttgttt
    45661 atgatggtaa ctgaataaac tttcctgaga tggtcaatgc tttccttgaa agtcttacag
    45721 atattctttc ttcatagaaa gtttaagttt tatagttagt gtacaataaa agtatgttta
    45781 taccatatgg tgtccggata atcagtcatt taaacaatga ggttcaaaga tatgaaaagg
    45841 ggaaatttga tgaagaaaat aagcaatgaa atccaaattc tggcagaaac tttaactctt
    45901 cccaaagtcg ttatagagca aatgattgta caagcaaaaa acatccttga tgaggtgttt
    45961 gagcaatata gtcatgaact aatggatcct aataaagcaa aggcgactgc aaagaaaata
    46021 gacaaagtct atgaagagga accttttgca gcattagcta tttatctata tgcagcttct
    46081 caaagttgga aaaaaattta tcaaccattg aagattccaa gaactatcta tttggcaaca
    46141 atgaatgctt ttactagatt tattcatgaa catttccaag taacaggaag ctatcgcttt
    46201 gaccgtggtt tttggatttg gcattacact agtggcttga tctttcggat tggtgaattg
    46261 gagtacgaaa tgacatattt tccgaaaggg tacacggatc taagattaga aggaaagcag
    46321 tgcctttctc ttcatattcc ttccgatgca gatttatctc agaataagat aagagatagt
    46381 tacgaaagtg ccattcgttt ttttgaactt tattttcctg attaccatta ccaagtgatg
    46441 tacacagata cttggttact atcacccaat cttactaaat ggttgaaaaa agagtcaaaa
    46501 atctgtcttt ttgcagccga ttatcgatta ttatcagttg atgaacagga cgatagtgga
    46561 gtcccttgga tctttggacg agtagatgcc caaatacatg attatccaga aagtacgagt
    46621 ttacagcgac aagcaaaaga gcaattgctt gcaggcgagc acattggctc tggcttaggt
    46681 attcttactt cataagttta gtacttcgaa atagtttaaa agagcgtgag acaaaagtaa
    46741 agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt
    46801 cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt
    46861 tcttggagtt aaacgcttct gtcccagcct cttttttagt gttctatcga acacacagta
    46921 cgttcaatag gtaaactagc tataacaata gatattaaag ttaacggaca agagggcctt
    46981 ttttaggtag ctaaaacgta ataatgaaaa tttatttcca gagataggag tcaatcagct
    47041 agtctgatcg actgttttaa caagttgact ttcattttaa cttttgatac catttaaaac
    47101 acaaatcgta attgtttcga tttgtgtttt ttagcatgac tacaatcaaa gaagaaagtg
    47161 ggtcgtccat atggaaaaaa cagatatctc aagtgcatac cgccgcttga aaagcccaaa
    47221 tatcaaaaca cgaaaacgtg cgctgaagat catcaaagat gtgaaacgga atagtgggaa
    47281 aaggtaagaa gaaggaaact taattaaaaa aatagccgaa aaacgttttt cggctatttt
    47341 caataatcgg tttttgtgta aggtttccga aagaactgct gttgattttc catttttaac
    47401 atatgataaa ttggtagaat ctttcttttc aaattgatac cattccctaa ttccagttat
    47461 atagttaact acagaaccac aaagcacgta cgtttataaa agtattatct gtagttattt
    47521 ttcaattccc tttcaacctc ttattttgac cagtagtatt gatcacttta ccttttaaca
    47581 cctttgttcg tttttgaaac atttttagtc aagaatggaa acgtaactac ataatcaaag
    47641 gttttgcgaa atatttcatg aaatatttca tgtagaagcg agcaatacgg ttgcaaataa
    47701 ccattatcgt ttaataagca gttacgaaag ctcccaatat ctctaacgtt cagctcactc
    47761 catttctttt cttcaaaaaa atcagtgatt cgtatctctt tctatttgtc gaaaaagtta
    47821 actttaaaaa gtaacgccga aaaaatatgc aaaaaaataa aaaagcagtt actattgcta
    47881 gaataacatc aaggaataat aagttttggt tttcagataa tagtcgataa gttgtgtatt
    47941 ggaattttag atacagtaat aaagtatttt tcatgaaatt ttgtgagaat cactattaca
    48001 agtatcttta ataaaattaa tggttaagga ggaacttaaa gtagaatgta aatgaatagt
    48061 tgtagtcagt cgattgtgat actagactca aagttgcagg aataagtttt tcaatttgtg
    48121 attggaagat aaaaaatgaa aaagaagggc ttttttttat actttgttga ttgtagtgtt
    48181 tattttccct ctgtttagcc aagtaacatt ttctatgact caaccagttt caagtgagga
    48241 acatgataac ctttttcgtg ctacagaaga gtttaagaaa tatcaggaaa atattgttcc
    48301 ggagactcca ttagctagtg agccaattac tgatattgat ggaaaccaaa taggatggta
    48361 tatacagtac gaagttatgg tggtaaataa tgactcaata gtaaaaaata cagtaggctt
    48421 agagctaaca ccgattgaaa aggtgagttc attgttaacg tatatttata ctgatgaaag
    48481 tgacgagact gagatcattt taacggatta ctcaaaaatt gctactgata aaaagtatat
    48541 attgtggatg tagacactag tgaaatactt tatgatatag aaattgatga aaatttacta
    48601 gatgtagcac aagaaatcga tcatcaaaag caggaagcta ttcgatatgc tggtagaaaa
    48661 gggaaaagaa aatatttatt acgtgaatgt agcaaaagtt agagaagatg aaaatgagtg
    48721 gaaagaattc aagagtcgct actcaattaa ctcgactccg acatttacag tttacagaga
    48781 aggatcgata gaaaaaactg ttttttggac taaagaaagt ggaatgtctt tagctgaagt
    48841 agaagagttt ttggattatg ttagtatgca acagtaaggg agttcttatt atgaaaaatt
    48901 ttgacacata ttatgttatt gttttttcga tatctttgct gttaatatta ctaactttaa
    48961 tgcagtataa agagtatcca gagtatttta ttttaacttt tgtatattta gtaagtagct
    49021 acacacttct atataaattc agaagttctt ctagagtttc taaagtttgt ttcttaatta
    49081 gcttattgat tctataatat ttttgattta tataggaaat taaaaatatt atagaatcaa
    49141 cactcagaga acgggttggt ttttaataga tttaataagt actgataatg aaatttctcc
    49201 tggttgggaa aacgaagttg gttaccctgt agaagaattt tttcaagatt actttgcgga
    49261 gtatgccgat aggataatga gagattacgt aacagataaa actcaagaag tcatcaatca
    49321 atcccagtca ttaaatgaat tatctgataa ggcgaaagaa gaagcagttg ccattatttc
    49381 ttcagggatt agtgactata ttgatatgag tggtgaaatc aattatggcg ataaagacaa
    49441 aattgtacgc atgataaaag cttttatcaa aacttttgat gtgaaggcag atcttaagtt
    49501 taatgatact tacctagtag gaaaattaat taatatcctt gggacagaga aagatacgga
    49561 aacgtttatt tcctctttgt ctatgttgtt tacagataag catttttcaa ctcagcttgg
    49621 tggagaggaa ctagtggaaa tcttttaacc attacgccca gcattaataa aagtatatca
    49681 aaaaagtact cctgatgatt tttaagattg gtttgagaaa taatgtgaaa acactatttt
    49741 taatgttaat tatagataga gatgaacgcg tcatatggtt aaaagaatga aaaagtcaaa
    49801 gatacactgc tatatttcac taaggatata gcagtgtatc tttcatgtcg tttcactggc
    49861 agttttttat aaaaggaggt gtgcatttca gttgcttttc tttggtagtt agttgaatcg
    49921 tttaagaatt aacggatgtg gcagacaggt gctctgtcca aaaaccagta aaatctttga
    49981 tggttccatc tttatttagc tgtgcctgtt caaatactgt tttgttttta ccgaatggat
    50041 caattgcaat aattgttccg atccccgcac gtttggcagc aagtaatcct gagtaagcat
    50101 cttcgataac aagacaatca gccggagcta agctcagttt gtctgccgct ttaagaaaaa
    50161 tatccggttg cggtttccca gggaaagagc cgtcgtcata gacaatcttg tcccagtcaa
    50221 accagcgggc taaatcgaaa atatcaaaat aaaaagcaac attctctttg acagtagccg
    50281 tggcaatcgt taatggaatc tgttggtcag tcagctggtc aagagtcgct tcaagccctt
    50341 ttgtcaactg aagctcgttt tcattttgca agcaaagcat acgataatag ctttcttttt
    50401 cttctgacaa tacggcgatc tcagaggcgg tcaaatgctc tgaaatgaaa tgtttcaaaa
    50461 tctccgtatt cgtccgacca tgaatatttt ttaaaatatc ctcgtctgat aaaccatctt
    50521 ttgtatgatc atgtatcata tgcagccaag cagcttcatg cagatgagag tccaaaaaca
    50581 ttgttccgtt gaaatcaaat ataatccctt tcatagttac gctcctatag tggtattttc
    50641 ggttgaatca gcaatttgca gatcgacata ttgtgcatag tgctcttgaa cctcccgcga
    50701 gatctggcga tccgtaatga gcagatcgaa atcatgcaaa ttataaaaca cataaaaatc
    50761 atctcgattg aattttttat tatcaacgag tagatatttc ttacgggaat tatttagagc
    50821 gatctgctgt gcttctcctt cttcggtact gtaagtagag atcgcttcgt tgtgaacgcc
    50881 atttgaactg ataaatgctt ttgtaaactt tagtcttctt aaattgtcgt tggtgatcgg
    50941 tccgacaaat gcgccggtat ttttccggaa atcaccgcca atcagtagaa tttctggtcc
    51001 atctgcttga gcaagaatat caaaaactgg ataattattt gtaatgatcc gaattttctt
    51061 gttcagtaaa tgcgtcgcca atagttcgat tgttgtacct ggtcctaaga aaatcgtttc
    51121 tccctcgtga atcaaggagg ctgctgtggc agcaatttct tttttttctt gaatctgcag
    51181 agtggctttt tcagtgtgag aaagttcttg atctaaagtg taggaaagac tttgagcccc
    51241 tccatggaca cgcagcagct tgcccgattt ttctaattca tctaaatctc tgcgaacggt
    51301 catatcagaa acatctaatt gattgatgat ttcattaacg gtaaggattc cttttttatt
    51361 gaccatttct acgatagtaa gcaatctttc ttttttcaac attgtcttca cctctaattt
    51421 aaggataatc tatcatataa acggacaaca gtcaatctaa ttcgaacaaa agtaaacatt
    51481 tagggtggtt tttatgtttg ttacaataga ttttattgct gttttctggt ttttatgatt
    51541 atttgtgttt gtttttgttg acaaatgttt tggatgcgtt tacaatgcaa tcagttagaa
    51601 agagcgaaaa ctgattatag atttgaggag agatgtacat gcttaaatta ccagaagatt
    51661 ttatttttgg cggagccaca gcagcctatc aggttgaagg agccactaag gaaggtggca
    51721 aaggcgctgt tgcttgggat gactttttag aggaacaagg gcgattcagt cccgatccgg
    51781 caagtgattt ttatcatcaa tatgcaaagg acattgaatt gtgtgaacgt tttggtgtaa
    51841 atggtctaag attatccatt gcttggagcc gcatttttcc tgatggagct gggaaaccaa
    51901 atccagaagg aatcgctttt tatcatcgtg tgttcgaaga atgtaagaaa agaaacgtaa
    51961 cgccgtttgt gacgttgcat cattttgata cccctaaacg tttgtttgac catggagatt
    52021 ttttgaatcg agaaacaata gaagcatttg tttcctatgc cattttttgt tttcatgaat
    52081 tcaaagaagt caaggtgtgg agtaccttta atgaaatcta tccagttgca accaatcaat
    52141 atttgttagg tgttttccca ccaggaatca aatatgattt tacaaagatc gtcgcctgtc
    52201 ttcataatat gatggttgct catgcccgtg tagtgaacta tttcaaagaa aacgaacttc
    52261 cgggtgaaat tggtgtagtt cactcattag agacgaaata tgcggcaaca gacgcgccgg
    52321 aagataagca tgctgccttt ttagacgatg cattgtcgat ccgtttctta ttagatgcta
    52381 cctatttagg ctattattca actgaaacct tgactgctct tgatgagata tgtgaagcaa
    52441 atcaggccag ctatcatttt cctgaagaag atttcgttga attgaaaaaa gcatccacgc
    52501 gtaatgacta cttagggatc aatcactacc aatgccattt tgtcaaagct tacgatggag
    52561 aaaatgcaat ccatcataac ggtacgggtg aaaaagggac ctctgtttat aaagtgaagg
    52621 gaatcggaga acgtatttat aaagaaggaa ttcctcgaac tgattgggac tggctgattt
    52681 atccagaagg tctatatgac ttgttgctgc gaattaaaag cgattaccca cattacaaca
    52741 aaatctatat cactgagaac gggatggggt ataaagatca gtttgaagat ggcattatta
    52801 tggatcagcc gagaatcgac tatttgagag tctatttgga atccttaagc aaggcgataa
    52861 cagccggggt aaatgtgaag gggtattttt tgtggtcgct tatggatctt ttctcatgga
    52921 caaatgggta taacaaaagg tatggcttgt tttatgttga ttttgaaacc cagaagcgtt
    52981 acccaaaaga atcagcttac tggtacaagt tggtcagtga aaccaaaacg attatttaat
    53041 aagtgataat ggaggaagaa aagatgaata aagaggaatt acaaatgctt ggttttgaga
    53101 tcgttgcata ttcaggcgac gctcgcagta cattattgaa attattgaaa gaagtaagac
    53161 aaggaaactt tgaacatgtt gattcggcgt tgaaagaagc cgacgaaaat ttaacccttg
    53221 cacataattc gcaaacaaaa attttagcgg aagaagcatc tgggaaagag atggagatgg
    53281 gattcatttt tatccatggg caggatcatt taatgaccac tttattgctg agagatctga
    53341 ttcaagactt tattgtactg tacagacaga atcaaggata ggagaagaac gctatggatg
    53401 gaatcattaa acaaatcgaa aaagggaaac cattttttga aaaaatctca cgtaatagtt
    53461 atttaggagc cgttcgtgac ggatttttaa cagcgatgcc agctatttta ttttcaagca
    53521 ttttcatttt gattgctgcg gttcctgaga tttttggtta ccaatggcct gaatctgtct
    53581 ccacatggct atggaaagtc tataattatt cgatgggtgt tgtagggtta ctcgtttcag
    53641 caaccactgc tcgctgcttg gctgattcga tgaatcgtcg cttaccgaaa aacaaagtaa
    53701 tcaacaccac ctctgtgatg ctggcctctg tggtaagttt cctgttgtta agcgtctctc
    53761 cgattgaagg tggattttca acggaatata tggggacaaa gggactactg gcttcctttg
    53821 tcgctgcatt tttaacagtt aatttatata agttttgtgt gctgaaagat attaccatta
    53881 aaatgcctaa agaagttcct ggaacgattt cgcaaacctt ccgagatatt tttcctttct
    53941 ctttttcagt atttgcggcc gttttaattg atttaggcat tcgacaaatc ttccaaatct
    54001 catttgcaga agcaatcatt acggtgctgc agccgctatt tacagcagct gacgggtact
    54061 taggaatcgc tattatctgg ggagcgatgg cgctgttttg gtttgtcggt gttcatggac
    54121 cgtcgatcgt ggagccggca attgcggcaa ttatctatgc caatgtggaa accaatctgc
    54181 aattgttcaa ttccggtgaa caagcttcta aagtactgac ggtgggttta gggaattttg
    54241 taggaactat gggaggaact ggggcaacat tggttgttcc ttttatcttc ctactctttg
    54301 ctaagtctaa gcagttgaag gcagttggga aagcttcctt tattccagta agtttcgctg
    54361 tcaacgaacc attgctattt gcggcaccca tgattttgaa cccttatttt tttattccat
    54421 ttttgttaac acctgttgta aacgtttgcc tgttcaagtt ttttgtcgat gtattgggca
    54481 tgaacagctt tatgtacgtt ttgccttggg caactccagc accgatcggt ttggttctag
    54541 gaacgggcat gggtatctta gcttttgttc tagcagcggt tctaatcgtc gttgacttcc
    54601 tgatctatct accattttgc aaagcatacg atgctgtctt agtagagaaa gaagcacaaa
    54661 aagcagaggt gatcgcaacc gaggaaggtg cttctgccga tgtacgtgag gaagtacctg
    54721 tggcagcgag tcaagttatc agcgatgcaa ataaagaaat caatgtgttg gtactttgtg
    54781 ctggcgcagg gacaagtgcg atgttagcta atgctttaaa aaaaggtgca tctgaatcga
    54841 aagcaccgat cagtgcaatg gcgggtgctt atggttctca ttacgaaatg ctgcaggatt
    54901 ttgatatggt ggtcttagca ccgcaagtca attcttacta tgatgatatc aaagaagaca
    54961 ccgatcgctt aggaatcaag ctggttgcga caaaaggagc agaatatatc ggattaacga
    55021 gagatcctga aaaagctgtt cagtttattc tgaaccaatt tcaataaagg aggagccata
    55081 atgaaaatgg gaatttggtt gcgtaaagct gttttatttg cagctgtgtt aggggctttg
    55141 ggggctgttt caaaaactgt tgaagcatct gccagcgaat ttattcgcgg agcagatatt
    55201 tcgatcctgt cagatatgga aaaatctggt gcaaaatatt atgaagacga tatttcaaaa
    55261 gatgccttag agattttaaa aaataatggt gtcaactatg ttcgactgcg tttgtggcag
    55321 gatccttata atagtaatgg agaaagttat ggagcaggga caaatgatct ggaaacgacg
    55381 attgaccttg caaaacgagc aaaaaatctg ggacttaagg tgctgctgga ttttcactac
    55441 agtgattttt gggttgatcc tgggaaacag aatcttccta aagcatggca aggtctgacc
    55501 tttgaagaaa tgaatacggc attgtacgac tatacaaaga acgttctgtc ggaaatgaag
    55561 cagctggacg tttatcctga tatggttcag atcggcaatg aactgaatag cggaatgctc
    55621 tggccttatg gcaagtcttg gggagaaggc gggggagaat ttgatcgatt ggctgctttt
    55681 ttgaagtctg gtatccaagc tgttcgagac actcaaccta aaacaactcc agtaatgctc
    55741 cacttagctg atggtggcga tacaggagca tttacttggt ggtttgatga aatcacgagt
    55801 cgaggtgtgt catttgattt aattggagtg tcgtattatc cgtattggca cggttcaatg
    55861 aatgaacttc aaaccaatat gaatgcgatc agccaacggt atggtaaaga agtgatcgta
    55921 gttgaaacag cttatgggca tacaacagcg aatgccgata ctatgccaaa tgcttttggt
    55981 gaagccgaag cagctgcggg gggatatgaa ccaacaccga caggacaagc agagtatttg
    56041 ctggatcttg ccgatcggat tcaggcagta ccgaataatc gcggggcggg cttcttctat
    56101 tgggaacctc tttggtacaa cggaaatgtg agttgggcaa cacaagcagg tatgaattat
    56161 ctcagtgttc aatccactat gggaaacgaa tgggacaacc aagcgatgtt tgattttgct
    56221 ggaaacgcct tgccaagctt gcgtgcgttt aaacaagcag gggcgcaaat aaatctagtt
    56281 aaaaatgcaa gtttcgaaag cgatggacca acgaccagtc cttccaattg gcaaacctgg
    56341 tttcaacaag gaaccgctaa gggtacgatc aaaacggaag cgggcacttt ttttggggac
    56401 tacaaactaa ctttttggca ggatcaagca tacgaggcat cagcttatca aaatgttagt
    56461 ggcataagca aaggaactta cacattgagt gcctatgtaa tgtctggggg agagtttgat
    56521 actaatcagt tatatatcaa aaattatggt ggagcggaac taaatactgc ggttgttaaa
    56581 acaagtgaat ggacaaaaat tacaatcaaa gacattccaa ttacaacagg tgtttgtgaa
    56641 attggggttt atagcaaggc ttcaggaaat gcttggacga acgttgattt tgtttcattg
    56701 gtgaaacaat agactgcgga tcgtttcgat tcaagatcgg aggttccctt cagccacatc
    56761 gttagcgtag aaggcaggaa tcgaacctgc acctcgcctt tgccaaaaat aaccagaaac
    56821 gatttccagt catttttacc aaaacgagta aagcaccaga cttcatctac actttgtatt
    56881 atgctaaaga gagttcaacg atttcgtaag aagcaatgaa aaaattgcaa aaagttgtgc
    56941 agataacata aagtttgttt aagaaagggt aaccttagaa ttccgtcgtg aatatgattt
    57001 gagcacttag atggatagaa aaaagttctc ggaaaacagt tcgtttttcg agaacttctt
    57061 tttatgataa gaatgaaaca aattaactta tattatagtc tcttttggta gggaaccatc
    57121 ttatttttaa tgaaaatcaa gcattttagc tattgaagaa tgttgattta tagggattcc
    57181 ccctccatat tatgttggct ctttattccc actctgtttt caacgtaaag tgtataaaac
    57241 agaatgcgat actatcaagg gagttaaaac aatatacaga acagtaaaac actataaata
    57301 aaaataaaag tgcaataaaa gtgcaatttt tgcacctaat catactctgt ttatatatga
    57361 aaactagatt ctgaatccaa tatttggtta acgagaggcg ttcgaatttg aacgtctttt
    57421 atatattact ttcaaataaa tatgaggtag aatactatct attttctttt taacccaatt
    57481 gttataatgg agagtgtaaa atattttgtg taaatagaaa aaaggaagtc ccttctgtag
    57541 aatagagtta ccacaacaca ttcacagaag agaggacttc cctatgaacg attttactac
    57601 agaaattcta aagactctag cgaacaaagg cgatttgaat gaattattcc gtgtccattt
    57661 ggagaaagca gtcaatacgc ttctcaaaac ggagttaacg gctttcctag attacgaaaa
    57721 gtatgatcgc attggtttta acacgggtaa ttctcgtaac ggctcctatg accgtacggt
    57781 caagaccgag tatggggaac ttcatctcca gattccgcgc gaccgcaatg gtgagttcaa
    57841 gcaacagact gttcctgctt atagacggac gaatgacacg ttagaggaga ccgtcattca
    57901 cctcttccga aaaggtatta ccatgtcgga aatcgcagac ttgattgaga aaatgtatgg
    57961 gcattactac acgccccaaa ccatgtccaa tataacaaaa tcatttacag aagaggtaac
    58021 ggcgtttaat gggcgggagc ttcatgaccg ttatgctgct atttatatgg acgcaacgta
    58081 tattccgtta aagcggaaaa ccgtcgccaa ggaagctatt catatcgcag ttggcattcg
    58141 cccggacgga tcaaaggaag tattgagcta tgcgattgca ccgactgaat ccatcacgat
    58201 ttgggaggaa attttattgg accttcaaga gcgcggtttg aaaaatgtcc tcctgttcat
    58261 cacggatggc ttaaagggga tggtaggagc gattagtcgg ttctatccca aagctcgttt
    58321 tcaacattgt tgtgtacacg tttcccgtaa tatcagtcat aaagtgcgtg tcgatgatcg
    58381 taaggaagtc tgtgatgatt ttaaaatggt gtatcaagcc tcatctaaag aggtggcgtt
    58441 ggaagcacgt ggtacttttg cggaaaaatg gaaaaccagc tatccaaaag tggttgaatc
    58501 gattctttcg aacgatcact tgctcacttt ctatgatttc cccttggcca tacgcaagag
    58561 tatttattct acgaacttga ttgaatcctt taataaacaa atcaagaaat acagccaccg
    58621 caaggaacag ttccaaaatg aagagtcgat ggaacgtttc ttagtctcgt cttttgatac
    58681 ttacaaccaa aaattcctag gtcgcagtca taaaggtttc caacaggccg aaggcgagct
    58741 cgaacaaatg ctaagccaac tgattgagaa ttagaaacaa tctgcagtag ggaagaatca
    58801 tttacacaaa attattgacg ctcccttata atgagaactt ttactaacat aatttatgat
    58861 aaacttacta tataatcata ttaattaaat agttaggaga tctgcttatg tttatatcta
    58921 ctcttcaaat tcgaaatttt agaaattatg aatccgaaat ctttcgattc gataatgaga
    58981 caaatactat tataggtgag aatgattcag ggaaaaccaa tgctcttact gcattgagaa
    59041 tcttattaga tgatagttat tactatagta gtaaaacact aaaagaatca gatttttttc
    59101 atggaattca aaatgggtgg caagggcatt ggattattat ctctgcgaca tttgaaggaa
    59161 tcagcgaaga agaatttgac aatgagattt gtgcttccat ttctttaaat tctgaaagtc
    59221 aaacaatctt agaagaattg atttcaaatg cagataaagg tgtaggtagt atttctctat
    59281 ttattagacc taacaaagct attagaaagc aattatttga tatttccact gaattagact
    59341 ctcatcaatt taacgaattt agaaattcta ttcgtctatc cgactatgaa ttttactaca
    59401 catctaaatc aaatctaaat ttctgtatca atgaaaatta tgataaattg gtaggaagac
    59461 tcaatgacaa tatagctagt aatcctgaag aagacgatga agctctgttg ggctcaagaa
    59521 ttgacatgtc agatattttc aagcatattt cggttgttta tgttgacgct cttcgagatg
    59581 ttcttagaga aatgaaaaac aatcgaaacc cagtcaaaag aatcatggaa acaattgaat
    59641 caaaaatatc atcagataat gttgagtcat taaaaacaat tatacagcaa ttaaatgaga
    59701 ctattaccag tgttcctgaa attaggaaaa ttggcgaaaa tattaatcgg caactaaata
    59761 gcataattgg ttcagtgtat tctcctaatc ttcttctttc ctcaactatg tcagatgata
    59821 tgggttcttt agcaaaattt ctttcaatga agccagaaca aaatatcgac ttagacttac
    59881 tagggttagg acatctaaat atgatttatt tagctttgaa aatagtcgaa tttgaagcgt
    59941 gtcgttcaag agaactactt aatattatgt tgattgaaga accagaagct catattcatc
    60001 atcatattca aaaaactctg ttcgaaggat taaatcttca aaaaaactat actcagattc
    60061 tcatgaccac acattctgta catttagctg aagtgtctga aatatctcgg atgaatgtat
    60121 taaaatcgtt taatggcaaa tctatcgtta tgaatccagc aaataatctg aactcatttg
    60181 ctaaagaaaa gttaaaaaag aacaatctaa atctaataaa cgcagtagag agatatttag
    60241 atgttaaaag aaatggattg cttttttcta aaggtgttat cttagttgaa ggagatgctg
    60301 aagaaatact tattcctcaa atagtaaaaa aattatttgg tataaactta gacgaacttg
    60361 gaataggttt gattaatgtt ggtagtactt cgtttgaata tatcgctagc ttgtttgacg
    60421 atgatagaat acagagaaag tgtgctattg taactgattt agatgaacag gcaatcccat
    60481 cagaacatcg attgtataaa agcactgcac aagagaaagg tcaagcaaga aaagaaaaat
    60541 tatcctccct ttttaatgaa aacaattggg ttaattgttt ttatgctaat acaacatttg
    60601 aaattgagtt tttagatgtg aataaagaaa atattctaac ttatgtcagt ccaattatag
    60661 atgacgcatt caaagataaa aaaacaataa ctgcttataa agaagaaata caaagtgaac
    60721 aatatttaga aagaaattta gcgatgttaa aactagccaa tcatgttggt aaggggtggt
    60781 ttgcaattga gttaggtgaa aaaattgact ttttaatcaa aattcctaag tatattctta
    60841 atgctattgc ttttgcatgt caggaaatag tagatctctc aatttataaa aaaattctac
    60901 tccatacaat cgacttgtac gaggaaacta cagaattgtc agctttatca gctagtttaa
    60961 aaacaagtga tacagctgat ttgaatatag caataatttc tttattagag tactttggta
    61021 aagatgagga tgttgtgtat ctaatcaatc agatagaaaa ttacacaaaa atactttatg
    61081 ctgaggtttc aaaatgacaa atatcttcga aaaaatcgta aatgatttaa atttagaaca
    61141 aaaagatgtt gtggaaagtg aagataacct ttatgtaaat gcatgtcctg ggagtgggaa
    61201 aacaagggta cttacaagga aaattgctta ccaagcaatt aaacacgatg gatcactaaa
    61261 aagaataatt gctatcacat acactaatag agctgctgaa gaaattaagg aacgcctaag
    61321 tttacttagt atagatgatg atattaatat ttgggtgggg acaatacatc agttttgtct
    61381 tgaatttatt atttatcctt ttaaaatgaa tctctcaaga gtatcaaaag gatttacaat
    61441 tattgatgat tatactcaaa gagcttacgt cagccaaatt aataatatgc tgaatttaaa
    61501 tctcaaaacg tatgaactga acaaaattga tttgtcactt tctcatgatt ttaaaattaa
    61561 tgaagttcag tatactcaag tttctgaaat gtatcacact atgttggaaa aaaataaaga
    61621 aattgatttt gatatgattt tactagtagc gtataaaatt ttaactgtaa acagtaacgt
    61681 tgcacgtaat attgctagta atataagggc gatttatgtg gatgaatttc aagatactag
    61741 agaattacaa tacaatgtaa tagctaaatt acttcaaaac aaccctcaaa ttcagtcaat
    61801 gtttgttgga gatattgacc aagcaattta tggatcatta gatggtatag ctaaatcagt
    61861 tgaagaacta gaaatgctaa ctagtcacac ttttcaatca aagacgctac atggctgtta
    61921 caggtctaat cagagattag tagacttcta ctctaatttt cagagttgtc cttacgagat
    61981 tgaaagcaga gggaataata aaaacgaccg tggttttata agttatgatt gcaaagttac
    62041 taaggaggat ttacccaaca taataaaaaa tataataaag gaaaagattg aaagcggcat
    62101 tcatgaaaaa gatatttgtg tacttgctcc tcaacactat cctctgtttt cactgggtga
    62161 gtatttaaag aaagaattac cgtattgcaa ttttgatgct ataaatatat caccaattaa
    62221 ggtaaataat catagtttat ttttcaagtt agcaattttg tactttactg aatcaggtga
    62281 aaaggttcga agaagaaagc taattgcaaa cgatatttta att
//
LOCUS       DAJSAW010000010        61304 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJSAW010000010 DAJSAW010000000
VERSION     DAJSAW010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.010"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..561)
                     /locus_tag="ONT64_000707"
     CDS             complement(<1..561)
                     /locus_tag="ONT64_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCR2302145.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(748..1398)
                     /locus_tag="ONT64_000708"
     CDS             complement(748..1398)
                     /locus_tag="ONT64_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302146.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="ONT64_000709"
     CDS             complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="ONT64_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCR2302147.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2910..3908)
                     /locus_tag="ONT64_000710"
     CDS             complement(2910..3908)
                     /locus_tag="ONT64_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCR2302148.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="ONT64_000711"
     CDS             complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="ONT64_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR2302149.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4924..5655)
                     /locus_tag="ONT64_000712"
     CDS             complement(4924..5655)
                     /locus_tag="ONT64_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HCR2302150.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5659..6924)
                     /locus_tag="ONT64_000713"
     CDS             complement(5659..6924)
                     /locus_tag="ONT64_000713"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HCR2302151.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7187..10003)
                     /gene="uvrA"
                     /locus_tag="ONT64_000714"
     CDS             complement(7187..10003)
                     /gene="uvrA"
                     /locus_tag="ONT64_000714"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR2302152.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10013..12007)
                     /gene="uvrB"
                     /locus_tag="ONT64_000715"
     CDS             complement(10013..12007)
                     /gene="uvrB"
                     /locus_tag="ONT64_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCR2302153.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12266..13768
                     /locus_tag="ONT64_000716"
     CDS             12266..13768
                     /locus_tag="ONT64_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HCR2302154.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13770..14507
                     /locus_tag="ONT64_000717"
     CDS             13770..14507
                     /locus_tag="ONT64_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR2302155.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14552..14725)
                     /locus_tag="ONT64_000718"
     CDS             complement(14552..14725)
                     /locus_tag="ONT64_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302156.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14701..15366
                     /locus_tag="ONT64_000719"
     CDS             14701..15366
                     /locus_tag="ONT64_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2302157.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(15448..16614)
                     /gene="dnaJ"
                     /locus_tag="ONT64_000720"
     CDS             complement(15448..16614)
                     /gene="dnaJ"
                     /locus_tag="ONT64_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR2302158.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16765..18594)
                     /gene="dnaK"
                     /locus_tag="ONT64_000721"
     CDS             complement(16765..18594)
                     /gene="dnaK"
                     /locus_tag="ONT64_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR2302159.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(18645..19208)
                     /gene="grpE"
                     /locus_tag="ONT64_000722"
     CDS             complement(18645..19208)
                     /gene="grpE"
                     /locus_tag="ONT64_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR2302160.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(19302..20345)
                     /gene="hrcA"
                     /locus_tag="ONT64_000723"
     CDS             complement(19302..20345)
                     /gene="hrcA"
                     /locus_tag="ONT64_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HCR2302161.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(20579..21745)
                     /locus_tag="ONT64_000724"
     CDS             complement(20579..21745)
                     /locus_tag="ONT64_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR2302162.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(21761..21988)
                     /locus_tag="ONT64_000725"
     CDS             complement(21761..21988)
                     /locus_tag="ONT64_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR2302163.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            22472..23062
                     /locus_tag="ONT64_000726"
     CDS             22472..23062
                     /locus_tag="ONT64_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HCR2302164.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            23252..23965
                     /locus_tag="ONT64_000727"
     CDS             23252..23965
                     /locus_tag="ONT64_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302165.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(24080..25090)
                     /locus_tag="ONT64_000728"
     CDS             complement(24080..25090)
                     /locus_tag="ONT64_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HCR2302166.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25127..25609)
                     /locus_tag="ONT64_000729"
     CDS             complement(25127..25609)
                     /locus_tag="ONT64_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HCR2302167.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            25759..26127
                     /locus_tag="ONT64_000730"
     CDS             25759..26127
                     /locus_tag="ONT64_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HCR2302168.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            26592..27044
                     /locus_tag="ONT64_000731"
     CDS             26592..27044
                     /locus_tag="ONT64_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2302169.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(27168..28019)
                     /locus_tag="ONT64_000732"
     CDS             complement(27168..28019)
                     /locus_tag="ONT64_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HCR2302170.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(28032..28817)
                     /locus_tag="ONT64_000733"
     CDS             complement(28032..28817)
                     /locus_tag="ONT64_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2302171.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(29169..30110)
                     /gene="ribF"
                     /locus_tag="ONT64_000734"
     CDS             complement(29169..30110)
                     /gene="ribF"
                     /locus_tag="ONT64_000734"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCR2302172.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(30114..31037)
                     /gene="truB"
                     /locus_tag="ONT64_000735"
     CDS             complement(30114..31037)
                     /gene="truB"
                     /locus_tag="ONT64_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR2302173.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(31405..33767)
                     /locus_tag="ONT64_000736"
                     /pseudo
     CDS             complement(31405..33767)
                     /locus_tag="ONT64_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            34215..34388
                     /locus_tag="ONT64_000737"
     CDS             34215..34388
                     /locus_tag="ONT64_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2302174.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            complement(34545..34892)
                     /gene="rbfA"
                     /locus_tag="ONT64_000738"
     CDS             complement(34545..34892)
                     /gene="rbfA"
                     /locus_tag="ONT64_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR2302175.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(34919..37225)
                     /gene="infB"
                     /locus_tag="ONT64_000739"
     CDS             complement(34919..37225)
                     /gene="infB"
                     /locus_tag="ONT64_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR2302176.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(37238..37549)
                     /locus_tag="ONT64_000740"
     CDS             complement(37238..37549)
                     /locus_tag="ONT64_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HCR2302177.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(37546..37839)
                     /locus_tag="ONT64_000741"
     CDS             complement(37546..37839)
                     /locus_tag="ONT64_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCR2302178.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(37861..39036)
                     /gene="nusA"
                     /locus_tag="ONT64_000742"
     CDS             complement(37861..39036)
                     /gene="nusA"
                     /locus_tag="ONT64_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR2302179.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(39059..39532)
                     /gene="rimP"
                     /locus_tag="ONT64_000743"
     CDS             complement(39059..39532)
                     /gene="rimP"
                     /locus_tag="ONT64_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR2302180.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(39677..44035)
                     /locus_tag="ONT64_000744"
     CDS             complement(39677..44035)
                     /locus_tag="ONT64_000744"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HCR2302181.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(44247..45956)
                     /locus_tag="ONT64_000745"
     CDS             complement(44247..45956)
                     /locus_tag="ONT64_000745"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR2302182.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(46024..47292)
                     /gene="rseP"
                     /locus_tag="ONT64_000746"
     CDS             complement(46024..47292)
                     /gene="rseP"
                     /locus_tag="ONT64_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCR2302183.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(47453..48253)
                     /locus_tag="ONT64_000747"
     CDS             complement(47453..48253)
                     /locus_tag="ONT64_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR2302184.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(48250..49062)
                     /locus_tag="ONT64_000748"
     CDS             complement(48250..49062)
                     /locus_tag="ONT64_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCR2302185.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(49257..49814)
                     /gene="frr"
                     /locus_tag="ONT64_000749"
     CDS             complement(49257..49814)
                     /gene="frr"
                     /locus_tag="ONT64_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR2302186.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(49817..50539)
                     /locus_tag="ONT64_000750"
     CDS             complement(49817..50539)
                     /locus_tag="ONT64_000750"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR2302187.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(50675..51556)
                     /locus_tag="ONT64_000751"
     CDS             complement(50675..51556)
                     /locus_tag="ONT64_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR2302188.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(51655..52437)
                     /gene="rpsB"
                     /locus_tag="ONT64_000752"
     CDS             complement(51655..52437)
                     /gene="rpsB"
                     /locus_tag="ONT64_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR2302189.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            52796..53275
                     /locus_tag="ONT64_000753"
     CDS             52796..53275
                     /locus_tag="ONT64_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HCR2302190.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            53492..54583
                     /gene="phnW"
                     /locus_tag="ONT64_000754"
     CDS             53492..54583
                     /gene="phnW"
                     /locus_tag="ONT64_000754"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HCR2302191.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            54576..54704
                     /locus_tag="ONT64_000755"
     CDS             54576..54704
                     /locus_tag="ONT64_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCR2302192.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            54708..55253
                     /locus_tag="ONT64_000756"
     CDS             54708..55253
                     /locus_tag="ONT64_000756"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCR2302193.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(55707..57398)
                     /locus_tag="ONT64_000757"
     CDS             complement(55707..57398)
                     /locus_tag="ONT64_000757"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR2302194.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(57818..58765)
                     /gene="arcC"
                     /locus_tag="ONT64_000758"
     CDS             complement(57818..58765)
                     /gene="arcC"
                     /locus_tag="ONT64_000758"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR2302195.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(58880..59899)
                     /gene="argF"
                     /locus_tag="ONT64_000759"
     CDS             complement(58880..59899)
                     /gene="argF"
                     /locus_tag="ONT64_000759"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR2302196.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(59990..61219)
                     /gene="arcA"
                     /locus_tag="ONT64_000760"
     CDS             complement(59990..61219)
                     /gene="arcA"
                     /locus_tag="ONT64_000760"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCR2302197.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT        17036 a        13093 c        10619 g        20556 t
ORIGIN      
        1 tataaacatc accccaaata aaacagaaaa cagtataagc attataaaaa ttatcaacaa
       61 ccttgatcct tcttttatag attcaggaaa cagtataatt ttagtactag ataaattgtt
      121 tacaccgatt agtgttaaaa tatctttatt cttcttgcta acataaccct ttatgcacaa
      181 aagaacagct atacttaaag taacaaataa ataacgtatt ccaatagaaa aatcactata
      241 aatactctct agcataggac cgacaattga agaagtcact actgcaaatg ctggacctat
      301 ccaagcaaca ttagaatgtt ctttctctct tttcctttgt aaatctttgt tgagtattaa
      361 tctatttatt tcttctgcag gcaagttaat tttcataacc ttatggggga ttatccaatt
      421 aaaaatcgga aaaaggtagc ctccccaaaa agtatagtga tctgtgtcaa tcagataata
      481 acctgatttt gattccagaa taacatatct caaagccgaa cgaaaatttt tcatgaatat
      541 ccataatctc actgtttcca cacctaattc ctccaattcc aattaaatgg atttatagca
      601 tcaccaatta tgttaactgc ctgaccaacc tcattgataa aattggcacc actatcccat
      661 gcttcaacaa tgctatatat ctatcaaaca aaaaatggaa aactaaatac tcgcgcacat
      721 aaattttaca ttctgttttt tataagacta ataataatca ttgaaaaaat ttcttatcgg
      781 tggcgcatat tcccaattga ttaatgtgat cccatattct gcaagtattt tacgagcttc
      841 ttctaagtca tagtgagaaa aatgcataaa acctgcaata cgatcacgga ctgtgagctg
      901 tggcttgctt ccaccatcat tcataaaata tccagcagag ccactactta aagtgattgt
      961 tacatcttca ctttctatta ttaagaattc ataatgaaat gctttcaact ctccactatt
     1021 atttaaaagt tttatactcc aacttcgtct aggttgagtt gtcacattcc ctctaaaact
     1081 tcctataaat tctgcaatca ccgtatcttc aatcaaaatt ctttcctcat ctatttcata
     1141 ttcaagacta aacggaaatt ctccatatgt aatctctggc aatggtggtg ctgtagttct
     1201 ccaaataaat acaataactg tgaatagaaa aaatccaacg atcgatataa atgttatgag
     1261 acctgctcga agtaaaaccc gatataataa aaatgctgta tttcgctttg ctatatctag
     1321 aaaaataaaa agtactgcaa tcataatgat agtaaaagtt gtaaaaaagc acattttttg
     1381 ccctcccttt ataaacatcg attctcaaat tctaaaccaa atcttgatag ttcaagttca
     1441 ggtataacgt ataccaacca tacatcccaa ttactattta tgaacttatg attatcactg
     1501 aataaatcac tccatcccct tgttaaaact tctacattaa ttacacctgg ttgttttcga
     1561 ctagctaatc caatgaactt atcaaccatc cttatccacc aaccacgaga atcttgacca
     1621 gaagtaagaa aaacagttct caaggttgat cgagcagttc cttccagttt ttaattaatc
     1681 tccagttata gataaatact tgatcctatc tatatcagga cttctatatt aagcaaaagt
     1741 ttatgtataa aactaaaatc ttcattcagt tgtctccccg ctttttatac gattgtatta
     1801 aaaaacaata gaagcctttc aacattatgt tactccagta aagttggaat tatagtttga
     1861 cggaagaaaa gcaagtatag cttttaaaaa aaactgatac attcgcatct cctgcttcat
     1921 gtatcagttt tttctttgga ttaaactgct tcttcacgca gtttttccgc aaattcattt
     1981 attttgcgaa ttcggtgatt aattcccgat tttgatattg cacctgaggg aatcatttcc
     2041 cctaattctt tcaagctgac ctctggatgt tgaagccgaa gttctgcgat ttcctgaagt
     2101 ttttcaggaa gggcatgtag accgaccttt gcttcgatca attcgatgtt ttcgatttgt
     2161 ttagaagccg catcaatcgt cttgttcaga tttgctgtct cacaattgac tagacgattg
     2221 accgaatttc tcatatcccg tacgattcga acatcttcaa atcggagcat tgagttggtt
     2281 gcaccaatca atgttaagaa atcagcaatt ttttctgctc cttttaaata cgaaatatag
     2341 ccattgcgcc gttcaagcgt tcgagcatgt agaccgtaat aatttaacat atcgcaaatg
     2401 tcttggttat gctgttcata aatagaaaat atttccaaat gataacgact cgtttccgga
     2461 ttattgactg aacctgaggc cataaaagca ccacgtaagt aagaacgcat tttttgcgaa
     2521 tttcccataa tctcgtccga aacgtgtcca ttgaacatca taccatccat gatatcaaga
     2581 tccatcaaca catcttttgt gccttgcttc aaccggacga tatagacatt gttctttttt
     2641 aatttcattt ttttacgaac gagtagctca cttcgtacct gataatgatc tttcaaaaga
     2701 gaatagatac gtctggcaat tgcagcattt tctgtttgga cgttcaaaat aaactgctgg
     2761 ttcacaatac ctaaagagcc gttcattcgc agcaaagcag ctaattccgc tttcgcatgt
     2821 tctcgatgaa cttccaaacc agttagttct ttcttcacat ctgcagcaaa tgacatccta
     2881 ttctcctcct ttctccaaca ataaattgct taatattttg aaccgaaaac taaacggaac
     2941 aattcttcta ctactttgtc tccatcgtgg aagacaccac catctttcaa ttctaaaaag
     3001 tcggtggaaa tcactcgaca tccttcattt ctcaatccgt tgaagtcgtg tgcgacttgt
     3061 actagatact catcatagat ctctgggtcc atataatctt caggcacttt ttccgtattg
     3121 accaatactg tatcgataaa aggctgattc aaatgcttgt gtaaaacacg tatatgatcg
     3181 gcatctgtga aatgttccgt ttcccctttt tgggtcatga tattacagat atagacggtt
     3241 tcagcagttg tcttgagcat tgcttcgccg atttctggta tcactaaatt tggaaggata
     3301 ctggtgaata aacttccggg tcccaaaacg accatgtcgg cgtccataat tgctgaaacg
     3361 acttttcgag ccgctttggc ttcactttta tcatttgtgt tgcagacata cacacggtca
     3421 atggttttac ggttgatcgc gattttagat tctcctactg cagtcgttcc gtctttaaaa
     3481 acagcatgca aggtcaaagg agtttctgaa gaaggataaa catgtccatc tacatgcatc
     3541 atttttgcta ataattgaat cgcctcataa gtgcttcccc gcatctcgga aactgcggca
     3601 atgatcagat taccgatcgt atgattggct aaaaactgat cttccttttt gaaacggtat
     3661 tggaaaatat cttcatagaa ttgtggcata tccgataacg ccactaagac atttctcaag
     3721 tcaccaggag gagccatagc gatcgattct ctgattgcac cgctgcttcc accatcatct
     3781 gcaacagtca ctactgctgt gatatctgct ccttgatttc gtaaactttt caggataact
     3841 ggcagaccag tgccaccgcc gaccactacg atctttggtt ttcttatccg gtaggttttc
     3901 attttcacga ccggttcacc gtctctttcc ttttatcctt gtcccgatgt gtcacattga
     3961 ctttatagtt tttccctaaa gcatttgcca aacgttcagt caatgccaca gaacggtgtt
     4021 gaccgcctgt acagccgatt gcaactgtca agctgctttt tccttcttta atgtatccag
     4081 gcaatacagt ttccagaaga tccaaaaatt tatggtagaa tgattgcgtt tcatcaaagg
     4141 acatcacata atcatagact ggctgatctt ttccggtcaa tggtcttagt tccgggatat
     4201 aatgagggtt gggcaaaaaa cgcacatcca tcacaatatc cgaatcgatc ggcaacccgt
     4261 atttaaaacc aaatgataca aattcgatcc gaaatccttg gtctcccttt gatttgaatg
     4321 actcattgat tttttcacgt aattgtcgtg gtgtcaatgt tgtcgtatca atgatcacgg
     4381 aagcttgggc tttcaagtcg tctagtatcg cccgttcttt acgtatccct tcagtgatca
     4441 aaccgtccat cgccattgga tgtgctcgtc ttgtttcttt gtagcgagcc actagttctt
     4501 cgtctgaagc atctaaaaat aagatagaag tgtcgatgaa gttcgtattt tcgatttcga
     4561 tcagcatgtt ttggatttct tcaaagaagg aacgagaacg caaatccact cctaaagcca
     4621 tttttgtgac tttacctgat tctttgatca attcccagaa ttttggaatc aatcgaggcg
     4681 gcatattatc gatacaaaaa tagcccatat cttcaaaact ttggatagct actgttttcc
     4741 ctgcaccact catacctgtt atgatgacta attgcaaatt atctgtcata tttacgaacc
     4801 cctctcacat tgtgcataat aaaattatat catcccgggc gtatcattac cagttttctt
     4861 tttcttttac aaaaagattt ggaccaaata tcttttagga tatttgcccc aaatccaatt
     4921 tttttacttt tccgatgctg tatccaatgc ctcgcttctt tcagctaatg cacgttcgat
     4981 cgttcgatcc gctaaaatag attgagcatc gatcactggg taatgattat cttcagcaaa
     5041 ttcatgcatc aaagataatt ctgtgcaacc taggatgact ttttcacagt ttaggcgctc
     5101 taccgcttcc tctaagattt cgtaatagag ctcttgattc aaataatccg attccttgat
     5161 ctcatgatag atcaggtaat tgattttttc ttgaagcgca gtatcaggaa tcactgtttc
     5221 gaaaccaaga tttttgactt cgcgttcata gattccagct ttcatacttc cttctgttcc
     5281 tagaatcgct actctccctg tcgtatgctg acggacaagt tcatttgctg cttctcttgg
     5341 catatgcaag ataggaatat ccgttgccgc ttgtaactct tcaaaaaagt agtgtgcggt
     5401 gttacaagtc aacacaatga agtttggtcg caataaattt tgtttctcaa tatcatccag
     5461 cagaaacggc attggatttt cttctgatcg atctaaaata taagctgtac gatcaggaac
     5521 tgttgcatga ttgaataaga cataattcaa atattcttga tcttttgtag ctttcgttcg
     5581 gtggttgatc aagcgaacaa aactttctgt cgccatcgtg cccattccgc ctaaaatact
     5641 gaaaaaattc tccatgaact agccttcttt cacatgaaaa tactttttga atcttggaat
     5701 atagttatgg aacatatatt tcatcaacaa ccagcgcttg attgatcggt ctttttcata
     5761 aaatacagta gtaccgtaac gattttcttt gatcatttgc tcagctagtt ttttgtcttc
     5821 attttcccga gcatattcta aaaagatttt tttgggcaca cccatccata atcttgcttt
     5881 atctgattga ttcgttccat aggtcgtttc aacaaagggt ttgttgaaca ctcgatcttc
     5941 tgtgacaaaa cgagccagat tcaagccgtt caatgtcaca aagaaactgc tacgaccttg
     6001 tctcaaattg atttcaaata atttatattc tccatctcta ggatcatact tcatatcgaa
     6061 attggcaaag cctgtatatt cgattttttc caaaaatgcc ttaatggttt gatagatttt
     6121 ttcgttataa tctggcatga tcacaacgta attaccaatc gatgctggtg taggatcttc
     6181 taatagtgga tgaccaaggc acatcatccg aacttgatga tcttcatcta catacgcatt
     6241 tagcacacgc atattactat catcacccgg gataaaatct tgtacaatca tttcactttt
     6301 ataaccggct tcgtatatcc ggcctaaaat taaatcgaac tcttctctag tctctaaaat
     6361 aaaagctttt tttcttcctt caaattggac agaaagatac tccacactgt ttgcaggttt
     6421 caaagcaact gggaattcaa aaggcaattc ctgttccaaa tgtccattta ctaacatttc
     6481 ttctctgacg atcaatgttt taggatacgg aagatcatat tcttcacaaa cttcataaaa
     6541 gcttacttta ttgatcagcc tttcaaataa agaataatca atatatggac agataaaata
     6601 ttctgacagc tcttgtttat gttgggagat caattctgca tatccatcac cacaagcaat
     6661 caaaagatat tttttactct tatcagaata tctttctttt gccaaacgaa gcatcgtctc
     6721 gataaataca gggtctttat caaatcctgg aataacttct acattgacaa ttttactgta
     6781 gcgggtaggg gctaactgat ccgaagcata tgcttggcag attttgccgt atgcttcatt
     6841 aaaagaacgt gccataccat aaacgttcat gtcgcttcct aataatatag ggataaattc
     6901 ttcattttca atactgttca tcattatctc tcacttactc ataatctctt gtttttcttt
     6961 tccaactaca caaggaatat cataccataa agaaaaaagc aatcaaagga aagtcctccg
     7021 attgctcaat tcttttttga acggattgaa cttataggaa gtttttatct ataccaatca
     7081 gtgtagaaag aactaccatg taatgattct agaacactga tattaataaa acaagcaaaa
     7141 attcattttt ttatgttttt ttaactatct cagctatcta tcctctttat accattattt
     7201 ttttcaaata gtgacctgta taactttctt ttacgttaat gatttcttct ggtgttccag
     7261 tcgcaaggat cgttcctcca ccttctccac cttctggtcc taaatcaatg atataatctg
     7321 ctgttttgat tacatctaga ttgtgttcaa tcactaaaac tgtgttacca gcatctacta
     7381 atctttgtaa tacatgcaac aagcgggcga tgtcatcgct atgaagtcct gtcgttggtt
     7441 catctagtat atagaaattc tttccattag agattttgtg caattcactg gcaagtttca
     7501 tccgttgtgc ctcaccaccg gacaatgtcg ttgctggttg ccccatcgtc acataaccta
     7561 agccaacatc aacaatcgtt tgcagtttgc gatgaatctt tggaatgtgc ttgaagaatt
     7621 ctacagcatc ctctactgtc atttccaaaa tatcagcaat gctttttcct ttgtaatgca
     7681 cttctaatgt ttcagaatta tatcgtttac catgacatac ttcacaagga acatagacat
     7741 caggcaaaaa gtgcatttcg atcttaataa ttccatcccc gcgacaagct tcacaacgac
     7801 cgccttttac gttgaaacta aaacgccctt tcttataacc ccgcattttt gcctcgttcg
     7861 tttgagcaaa taacccacgg atatcatcaa atacacttgt ataagtcgct ggattacttc
     7921 tcggcgtccg gccgattggg ctttgatcga tatcgatgat cttttcgata ctttcgtagc
     7981 cggaaattgt cttgaattta cctggcttag cagaattctt atttaatttt tgcgctaacg
     8041 atttctttaa gatactattg accaatgtac tctttccaga accagacaca cctgtgactg
     8101 ctacaaattc tcctaatgga aattcaacac taatattttt cagattgttt tctgaagcac
     8161 cagtgatctt gattgctttc ccattacctt tacggcgttc ttttggtaca gggattgatt
     8221 ttttccctga aagatatttc cctgtcaaag aattttcatc attagctact tcttcaggag
     8281 tgcctgcagc cacgatttgt ccgccttgtt ctccggcccc aggtccgacg tcaatcaaat
     8341 aatctgctgc ccacatcgta tcttcatcat gttcgactac aacgagggtg ttgccaagat
     8401 cacgcatttt ctttaatgag ccaatcagtc gatcgttgtc gcgttgatgc aatccgatcg
     8461 atggttcatc taaaatatac aaaactccag aaagattcga tccaatttgt gtagccaatc
     8521 tgattcgctg cgcttctccc ccagataatg ttccggaagc gcgactcaaa gttagatagt
     8581 ctagtcccac attttccaag aatgaaagtc tgtcatttac ttcttttaaa atcggtttgg
     8641 caattacttt ctcttgttca gataacgtta agtttgagaa gaaatctact gctttttgaa
     8701 tagacaactc atttgtttgc ccgatattcg ttccattgat ttgaacagat agagcttggg
     8761 gattcaatcg ataaccttta catgtctgac aagttaattc tgtcatataa agacgcattt
     8821 gctcacgcgt gaaatcactg ttcgtatcat gataacgacg ctcgatattc ttcaataccc
     8881 cttcgaaagt cgtttctaca tcgcgaacgc caccaaaatc attttcataa tggaaatgga
     8941 aaagatcatc tgtcccattc aagatgatct cctgatgctc ttccggtaat tcttcgaatg
     9001 gtgtatctaa atcgataccg aaactttcac aagcctgcgc taacatttga ggataatact
     9061 gagagctgat tggattccat ggaacgatag caccttgact aagtgtcttc gttctatctg
     9121 gaatcactaa atcttgatcg acttctaatt tgacacctaa accatcacag tctggacaag
     9181 ctccaaaagg cgcattgaat gagaacagtc ttggttctag ttcgccaaca gtaaatccgc
     9241 aataaggaca ggcatagtgt tcgctaaaca acatttcttc ttcaccgatc acgtcaacca
     9301 gagcatagcc gtctgctaat cgcagggccg cctctaaaga atcaaatagg cgagaacgaa
     9361 tcccttcttt cacaatgatc cggtcgatga cgatcgcgat atcatgtttt ttgtttttct
     9421 ccagctcagg cacttcactt aaatcataga tctcaccatc gactcgaacg cggacgtatc
     9481 cctctttttg gattcgttcg aatatttttt tatgttgtcc ttttttcttt accacgatcg
     9541 gtgccaacac ttggatcttt gtgcgctcag gcaaagccat tacttgatct gccatttgat
     9601 ctactgattg actggtaatc tcgatatgat cgttgggaca aatcggatgt cctacacgtg
     9661 caaacaacaa gcgtaaatag tcattgattt ccgtcacagt acctactgtc gaccgaggat
     9721 ttttacttgt tgttttctga tcaatagaaa tcgccggact caatccgtcg atgctatcta
     9781 cgtccggctt atccatttgt cctaaaaact gacgagcata agcagaaagg ctctctacgt
     9841 atctccgctg cccttcagcg tacagcgtat caaatgctag ggaacttttt ccagaaccgg
     9901 acaaacctgt aacgaccacg aatttatctc gtggaatcgt tacatctata tttttcaagt
     9961 tatgggcacg tgcaccatga atcgtaattt tatcgttagc catgttgtcc tcctactttg
    10021 aagcttttaa ttctaaaatc atatctcgca cattcgcggc tgtttcaaaa tccaaagctt
    10081 ttgccgcatc tttcatttct ttttctaatt tcatcaataa cgtgtctttt tcttccttcg
    10141 tcatttcttc gtacgagact tgtaccgctt cttttgtatc atcttccgat tctttcgtaa
    10201 tagaaatcaa atctcggatt tctttaatga tcgtttttgg aacgatccca tgttcttcat
    10261 tgtacttctg ctgaatcgta cgacgtcttg acgtttcatc catcgctaga cgcatagaat
    10321 ctgttacttt gtctgcgtac atgattactt taccttcttc attccgagca gctcgtccaa
    10381 tcgtttggac taaggaacgt tcactacgta aaaagccttc tttatccgca tccaagatag
    10441 caacaaggga aacttcaggt acgtctaatc cttcacgaag caaattgatt ccaactaaca
    10501 catcaaattc acctaatcga agatctcgaa tgatttctgt tcgttccagc gttttgatat
    10561 cactatgcaa atatttgact ttgatcccta attctttgaa ataatctgtg aggtcttctg
    10621 ccattttttt cgtcaatgtc gttacaaaca cccgctggtc tttttcgact cgttcattta
    10681 tttcgcctac tagatcatcg atttgtccca tgattggacg aacttcgatc actgggtcaa
    10741 gcagccctgt cggacggatg atttgttgga taacggtatc tgtttgttca tgttcatatg
    10801 gtcctggcgt cgcagagaca taaatgatct gattgacatg tttttcaaac tcttctaacc
    10861 gaagtggccg attgtctaaa gcagatggca aacggaaacc gtaatctact agcatctgtt
    10921 ttctcgctcg gtcaccatta tacattcctc tgatttgagg catggttacg tgcgattcgt
    10981 ccgccacgat caaaaaatct tcaggaaaga aatccaatag cgtataaggc ggttcacctt
    11041 ctttacgtcc gtccatatgc cgagaataat tttcaattcc cgacgtgtag cccatttcta
    11101 acatcatttc aatatcgtaa ttggttcgct gttctaaacg ttgagcttcc agaagtttgt
    11161 tttcatttcg caagacagtt aaacgctgtt ctaattccgc tttgatattc gctacggcat
    11221 gctccatatg ttcatcattc gtcacaaagt gagtcgccgg gaagattgct acgtgttccg
    11281 tttctcctaa aacttcacct gtcagtgcat tgacttctcg gatacgttcg acctcatctc
    11341 caaaaaactc cacacgtaag gcacgttcat ctcttgaagc cggaaaaatc tccacgacat
    11401 caccgcgcac tcgaaaacga ccgcgctgaa aatctatatc atttctttcg aattggatac
    11461 tgactaagtc acgaatcaac tgattgcgat ctagttctgc tccctgtcga atcgaaacta
    11521 cttgtttttg gtactcaaaa ggcgagccta atccaaaaat acatgaaacg gatgcaatga
    11581 cgatcacatc atttctttct aataatgaac tagtagccga atgtcgtaat ttgtcgatct
    11641 catcatttac acttgaatct ttttcgatat acgtatcgct tgaaggcaca taagcttcag
    11701 gctgataata atcataataa gaaacaaaat attcaacagc attgttaggg aaaaattcct
    11761 tgaattcccc gtacagctga ccagccaatg ttttgttatg ggcaataatc agtgttggtt
    11821 tattcacttt ctcaataaga ttggaaatcg tatatgtttt ccctgttcca gtcgcaccta
    11881 ataagatttg tgcttttttc ccgccgacta cgccatccac taattggtta atcgcttcag
    11941 gctgatcacc agccggttgg tatttcgaga caagctcaaa gtgtcgggag gtatctctct
    12001 caatcattct tcgtcctcct tttcattcat tccttttcat tttacgacca ataaaaagaa
    12061 aaggaaagca aaggcattcc ttactaaaaa attataccac accgaacata ctttcgccac
    12121 ttttttatct tattatacat gttatgtttt ataacataaa atgttagaaa ctcgcatttt
    12181 tttcattttt tcgagcttac agtcttgtct gattcagcac ttttcgccta taataataaa
    12241 atattggaaa tttaggaggt tgattatgaa aagaaagcat ttatttcact catttgtttt
    12301 cttaatgaca ggacttgtga catttgcgtt tggtcatgtt gcacaagctg acaacactac
    12361 aacggcagag tcaagcacaa gcgagacaat gcaaaaaata gagcccaaaa aagacgtata
    12421 cgtaattgcc agtgactctg cttttgctcc atttgaattt caaaacgcaa agggcgagta
    12481 cgaaggcatc gatgtaaaat tgatggaaaa catcgccaaa ctgcaaggat ttaacatccg
    12541 aatggactac cgtggtttct ctgctgcttt gcaagctttg gaatcaggac aagcagacgg
    12601 aatgattgcc ggaatgactg taacagatga acgaaagaaa agctatgatt tttctgattc
    12661 ttactttgat agcgggatcc aattagctgt caaaaaagga gatacttcga tcaagtctta
    12721 tgaagatttg aaaggaaaaa cagtcggagc aaaagttggt accgaaagtg ctgaattttt
    12781 aagcgaccat gaatctgaat atggctattc tatcaaatat tttgatgctg ctgatcaatt
    12841 gtacgacggg ttaaaagttg gcgcaatcga tgcaatgatg gatgattatc cggtaatcgg
    12901 ttatgcgatc aaacaaggac aagaattgga aacaccgatc aaaaaagaat ctggcggaaa
    12961 atatgcattt gccgttaaaa aaggacaaaa tcctgagctt cttaatatgt tcaatgaagg
    13021 attgaaagaa ttaaaaagaa caggcgaata cgataagatt attaaagaat atgtagcgga
    13081 tggttcagaa acttccagtg agtcttccac tgctgatgaa tcaacatttc ttggacttat
    13141 ccaaaacaac tatcaagcat tgttgaaagg actttggaga acaattctcc taactttgat
    13201 ctcctttgct ttggctatag taatcggtgt gatttttgga ttattcagtg tagcaccaat
    13261 acgcgctctt cgcacaattg ctagtattta tgtcgatatc atccgaggaa ttccaatgat
    13321 ggttcttgcg ttctttattt tctttgggtt gcctggtatc gttggattta cgattcctga
    13381 cttccttgct ggtgttatca ccttgactct taacgcaagt gcttatatcg cagagattgt
    13441 tcgaggcggg atcaacgccg ttcctgtcgg acaaatggaa gcttccagaa gtttaggtct
    13501 ttcttataat cggacaatgc aaaaaatcat tttgccgcaa gcgatacgca tcatgatccc
    13561 atcattcgtc aatcagttcg ttatctcatt aaaagacaca acgatcattt ccgcaatcgg
    13621 tgtagtcgaa ttgctgcaaa ctggtaagat cattgttgca cgtacaacac aaagtacgta
    13681 tgtttacttg atcattgcga tcatgtactt gattttgatc acagcattga caaaattagc
    13741 aaaagtttta gaaaagaagg tgaagtaaga tggcagaaaa aatcttagtt gagcatttag
    13801 tcaaaaaata cggcgataac acagtattaa acgacatcaa tgtatccatc aacgaaggag
    13861 atgtcgtctg tatcattggc ccttcaggtt caggaaaaag tacattcttg cgttgcttga
    13921 atcgtttaga agaaccttct gacggtgaaa ttattattga cggcgcccac ttgatggata
    13981 aaagcacaga tatcaacttg gtgagacaac atatcggaat ggtattccaa cactttaacc
    14041 tattccctca tctttctatt ttggaaaaca tcattttagc accgatggac ctgaagaaag
    14101 aatctagaga agaagcagag aaaaaagcaa tcaaattact gcagacagtt ggtctagaag
    14161 ataaaaaaga tatgtatcct gaaatgcttt ctggtggaca aaaacagcgt gtggctattg
    14221 ctcgtgctct agcgatgaac cctgacatca tgctttttga tgagcctact tctgcactcg
    14281 atcctgaaat ggtcggtgat gtcttgaacg tcatgaaaaa gttagcagaa caaggtatga
    14341 cgatggttat cgttacgcac gaaatggggt tcgctaaaga agtggccaat cgcgtcatgt
    14401 tcattgacga cggaaacttt ttagaagatg gaacgcctca acaaatcttt gaaaatccgc
    14461 aaaatgaacg gacaaaagat ttcttgaata aagtattaaa tatttaataa aaaccaaaaa
    14521 tgcacggtcg ccatgaaggg accgtgtatt tttatcttct tattttctca cctaatcgta
    14581 tcagccgcat cgcttgtttt cctgcacaaa caagcaatcg ctctacctgt tccatcgcat
    14641 aagaaagtac catcggttca ttgatgatat caagtacata gtcgatacca ccctcactta
    14701 attgagcttg atttttcttc tccatattat gtagttgtgg gacaactttc tgacaatcag
    14761 caattgtgtc aagaacggat caatccttta ttggagttgc tcgaaagggt cttctcttat
    14821 tacggacagg ctttgctcac tgtacataga caacaaatca ttatcctagt caatcggatt
    14881 tccagagcct cattgctatc attgcttgat aaaatccaga caaagtttaa taaaatgtat
    14941 cagttacagc taaactttgg aattgggtcg ctttgttata cagagcaaga gacaccgcaa
    15001 tctttccttc atgccaaaca agtttgcgaa tggatagctt tccatcaatc tgtgaacgag
    15061 atccgctttt ttgaagattt agacctcggg atcgttttac cagcaatccc gtcagatcaa
    15121 cgaacattat atgtaaaacg tattttgaaa agcctgacag aagaagaagt acatttgttc
    15181 aagaagactt tggcatgctt cagtaaaaat aacggaagca tcaaaaattg ctcagaagaa
    15241 ttatttattc ataaaaacac cttgcaatat cgattgaaca agttccattc tctcactggt
    15301 tataccccta gaaactatga cgattatcat attctaaagc tggctttttt gttggtacaa
    15361 acatgacaac tacaaaaaaa ccaggcgaac atatgattgt tcgtctggtt tttaattatt
    15421 aatgacttag cgtttttact agaaaaacta cttccgcttt ttcccgccaa aagcatcttt
    15481 aaacttatcg aagaaaccgt cttcttgctg ttcttcaact ttgatgccgc cagcttcagc
    15541 gaatgcacgt aatgcttctc tttgttgttc atttagattt ttcggtgtga tcagttttac
    15601 tttgacatgt tggtcgcctg ttgcaccacc acgtaacttc ggagcgcctt ttccacgtaa
    15661 acggaagttc gtacctgtct gtgttccagc agggatcttc aatttcacat ctccatggac
    15721 agttggtact tggacttcat cccccaaagc tgcttgtaca aaacttaaag gcaattcata
    15781 gtaaatttca gaaccttcac gatcaaagat atcactttct tctacacgga agaccacata
    15841 caaatcacca aatggtccgc cgttttctcc agcttctcct tgaccagcta aacgcatttg
    15901 ttgtccttct tctacaccag ctggtacatt cactttaact gaatgtgctt tcttctcatg
    15961 tcctgagcca tgacaggttg gacaaggttc tttgatttct ttccctgtcc catgacaaac
    16021 atcacaagtt tgacggctca tcactcgtcc aagtggtgtt tggcgttcta cattaatcgt
    16081 acctgcacca tgacatttat gacaagtagt tggttgtgtg cctggttttg caccgttccc
    16141 accacaagta tgacagattt cttcccgatt atacttgatt tctttttcta caccaaaaat
    16201 cgcttcttca aaagacaagt cgaccgtata ttgcaaatca gcaccttgac gaggcgcatt
    16261 tggatcgact gaccgaccgc ctcctccaaa gaaagagtcg aagatatctt caaaaccacc
    16321 gaagccgcca ccagagaagc cgccgaatcc accaaatccg ccaccagctc caccagcacc
    16381 gtaatttgga tctgtgcctg catgcccgta ttggtcatat gcagcgcgtt tttgcgggtc
    16441 acttaaaatc tcatatgctt ctgatacttc tttgaatttt tcttccgcat caggttcttt
    16501 attgatgtca ggatggtatt ttttcgaaag cttacgataa gctttcttta tttcatcttc
    16561 tgatgctcct tttgagagcc ctaagacttc ataataatca cgttttgttg ccataggctt
    16621 tcctcctatt ggttctcatc attttcacgt cgcttgttat catatcatat tttgtttgaa
    16681 acaaaaaccg atacttcaaa ctatttttca aaagaaaaag ccaaagtagc aactttggcc
    16741 ttctctcgaa attcagtgaa ctgattattt atcgtcgcca tcgacttctt caaaatcagc
    16801 atcaacgaca tcatctgctc cgccttgagc tgcttctggg ttttcttgag cttgttgttg
    16861 tgccgcttgt tcgtatagtt taacagtcaa gttttgaacg atttcgttca atgcatcgcg
    16921 tttagttttc atttcttcga tgttgtttgc ttcaacagcg gctttcaatt catcgcgagc
    16981 tgtttctgct tttttcactt cttcttcgtc cactttgcct tctaattctt tcaatgtttt
    17041 gtccacagag aatagcaatg catctacatc attacgtagg tcaacttcct ctttacgagc
    17101 tttatctgct tctgcatttg cttctgcatc ttttaccata cgttcgattt cttcgtctgt
    17161 tagacctgaa gaagatttga tcgtgatttt ttgttctttt tgtgtaccta gatctttcgc
    17221 agatacattt acaatcccgt ttttatcaat atcgaatgtt acttcgattt gtggaatacc
    17281 acgaggagct gctggaatat ctgtcaattg gaatcttcct agagttttgt tgtcagcagc
    17341 cattgggcgt tcaccttgta atacatggat gtctactgct ggttgattat ctgccgcagt
    17401 tgagaatact tgtgatttac ttgttggaat cgttgtattg cggtcaatca atttagtgaa
    17461 cactccaccc attgtttcga tacctaatga caatggtgta acgtcaagta aaacaacatc
    17521 tttcacatca ccagtgatca cgccaccttg gatagcagca cccatagcaa ctacttcatc
    17581 agggttaact gatttgtttg gttcttttcc tgtttcttta cgtactgctt ctacaactgc
    17641 agggatacga gtagatccac cgactaaaat aacttcgtcg atttctgatt gtgataaacc
    17701 tgcatctttt agcgcttgac gtactggtac ttttgtacgt tctactaagt cagctgttaa
    17761 ttcatcaaat tttgcacgtg tcaatgtcat ttccaaatgc aatggtccag attctcctgc
    17821 tgtgataaat ggcaagctga tttgtgtgct tgatacgcca gataaatctt ttttcgcttt
    17881 ttcagcagcg tctttcaagc gttgtaaagc cattttgtct tgagacaagt caatgctgtt
    17941 ttcttttttg aattctgcta ccatgtagtc gatgattttg ttatcaaagt cgtccccacc
    18001 tagatggttg tcgccagctg ttgacaatac atcaaagaca ccgtctccta gttcaaggat
    18061 agatacgtca aaagtaccgc caccaaggtc gaatactaag attttttcgt cgcgatctgt
    18121 tttatctaaa ccataggcaa gagcagctgc agttggttca ttgacgatac gttctacttc
    18181 tagaccagcg atcttaccag catcttttgt tgcttgacgt tgtgcatcat taaagtaagc
    18241 tggcacagtg ataactgctt tttcgacttt ttcacctaaa tagtcttctg cgaatccttt
    18301 gatatattga aggatcattg ctgagatttc ttgtggtgta tatgatttac cttctacatc
    18361 tactttgtaa cctgcttcac cgatatggcg tttgattgat gaaatcgtat ttgggtttgt
    18421 cacagcttga cgttttgcaa cttcccctac ttggatttct ccatttttga atgatacaac
    18481 agaaggtgtt gtacggtttc cttctgggtt agcgatgatt tttgcttcgt ttccttccaa
    18541 tactgctact gcagagttag tcgtacctaa gtcaataccg ataattttac tcataattaa
    18601 cgtctccttc tttaattgaa agtgtaagtt ttatatccgc acaattattg tgcaacaatg
    18661 accatggttg gtcttaacac gcgatcatgt aatttgtagc cttcttgtag tacttctaca
    18721 atcgtatcgg ctggtgtatc ttcagtcgct ggaacagttt gtactgcttg atgaagatta
    18781 ggatcaaagg cttctccttt agcagggatt ttttcaattc cttcttcttc cagtgcgtta
    18841 cgcaaacttt ccaagaccat ttccacgcct tttttcaaac ctgccccttg atcatctgac
    18901 acttctgtgg ctaatgcacg ttccaggttg tcaattgatg gaagtaattt tttggctaaa
    18961 tcttgtgaac ggtatttttg taattgctca cgctcatttt ttcctcgatt tgccatattt
    19021 gcgatttcag cacgagcccg caagtattta tcttccattt cttctaactc agcttttaat
    19081 ttcgctgttt cgaattcttc cgcttcagct tctgaaatac ccgaatcttc tgcagcttcg
    19141 acatcgattt ccggttctgg ctgagcatct gtcatttctt tatctagttc ttcttgattt
    19201 tctttcacga tacagtcttc ctttcattat tcagtcagct gtttgattgc tcttactaag
    19261 aaagagagaa cgatcagctg caaatagctt cgttattcac attacgagtt tgacaagtca
    19321 agcgaacggt agtaatcagc aagtttagct gctagttctc ttcgataaac atcgactaga
    19381 ccgaacatcc gagaataagg catactagta ggccctaaca aggctattgt acctcttcca
    19441 tgaccatcta tctcgtaact tgcttgaatc atactcatat tctgcaataa atcattgcct
    19501 aattcagcac caatcttgat atgaatattg ctatccatcg gagtgatcat ttgtgtaagt
    19561 ccgtctgagt ctttcataaa agaaaacatc gatttgaact gatcaagatc ttgacggggc
    19621 tcataatcca agagattcat ttgtccactg acataaacct tatcttcaaa aacatgacca
    19681 agaacggtgt caaataactg agacatccct tctgtcgttt ggaaatattt atgaagaatc
    19741 atcggaatct ccgtacgaag tttatggtac accgtcatca gaggatctcc gactagacgg
    19801 tcgtttacaa tctgtaccat tttttctagt tcctgacttc ccatatattc gggaagagtg
    19861 aacacttggc tttctacatt ccccatatct gttacgacga tagcaataat ctgtcgatta
    19921 ttcaagggta cgatacgaaa gccagtcagt cggcggtctt ttatttcagg tcccaatgaa
    19981 aaagtcgtat agcttgtcag ctcagataat atctcagcag attgcttaat aatctcattg
    20041 atttcatgaa attctctgcc tagagactga cgaatcagca gcaaatcatc ttctgctacg
    20101 cgagcaggtt ttaacaaatg atccacgtaa taacgatagc ctaaagcaga tggaatccgc
    20161 ccagaagaag agtgagtctt caacagcaat ccttcatctt ctaatgcttt catatcattg
    20221 cgaatcgttg ctgagctggc cttgactccc tcagacatca atgttttgga accaacgggc
    20281 acgccactac tcgtatagtg ttggatgatc agacgtaaaa tgtcgttttg tcgctttgtt
    20341 aacatcttcc tcacctctca ttagcactca tatattctaa gtgctaatcc acttataaat
    20401 atagcaagaa gcccttctcc tgtcaagaga aagactccat tttttagcac tctaaacaca
    20461 agagtgctaa ttctaaataa ttgatgtaaa tccagttata tcaacgtttc aatagtcctt
    20521 tattgtattt ttgaactttt tttatttaaa ttcagataaa aaacaaatat tctttaaact
    20581 atttttcaaa caaaaagcgc tcaaacacat tgttccccaa aaaggtccct cttggtgtca
    20641 aataaacacg gtcttcttca ttaacaagta acccttcttg ctctaattcc cttagcacat
    20701 caccgtacac cgattcaatt gttttttgat agcgatcata gaaatgtttt ttagtaatcc
    20761 ctgttttttt tcttagtcct aagaacattt cttcttctat ttgatttttt cgtgtcaatt
    20821 tttcttcttc caataccggg cgttcatttg ctcgtaatgg gcgtaaatag tgttggattg
    20881 gtcctttgtt gcggtaacga atatctccaa gataaccgct agcaccagct ccaaacccaa
    20941 aatagtggtc attgttccag tacatgaggt tatgttgaga ttcatgtccg gagagcccaa
    21001 agttacttat ttcatattgg tgacgtccat gcttttccat tgcttcaatc gtctcttcaa
    21061 acattctggt ttccgtttcc tgatcaggca gttcgagtct tccttgtcgt acccagttca
    21121 tgaacattgt tttgttttcg aggatcaatg agtacaaaga atagtgaggc agatccaaag
    21181 ccaaagcttt ttccaaagta tccagatatc cttctagtgt ttgtcccggt aatgcataaa
    21241 tcaaatcgat gcttacattg gtaaaatttt ctgcttctaa aaattgcata gtttgataca
    21301 catcttctgc ggtgtgttta cgtccaattt ttttcaataa gcgattatca aatgtctgca
    21361 ctcccatgga taaacgattg acgccatagt ttttcattac ttgaagtttt tcacgggtca
    21421 aatcacctgg attcgcttct acagtaaact cattaccatt ttgaaaaggc aggatctctc
    21481 tagcacctga taacagtcga tcaagttgtt tagcagataa agaagttggt gtcccgcccc
    21541 ctacgtaaag tgtttctaaa ttcgctgtcg gatgactttc tagcattatt ttcatttcat
    21601 tcagcagcat ttccacatac tcatctactg gctgcccttc taaaaaaact ttattaaaat
    21661 cacagtaata gcagatatgc tcgcaaaatg ggatatgaat gtacgcagaa accttgtttt
    21721 ctgcgggatt tattgtttct atcattgaaa ttctttctcc ttacgtcgaa aagtcgtgcc
    21781 tgttttcgac cttcttaaaa tcgcgttcaa gcaccaactc attgaaataa gccgcatatt
    21841 tagaatcaag gaaacctagc tctaaaaatg cttgcttatt gtcatcggag ctacacggtg
    21901 cttttacagc ctctcgataa acggtaatcg caaagctgac cttcggcatt aagtcaaaat
    21961 ctgcaaaaat atgaaaagct attttcacag attcctcctt aagtgctcag accacaacgc
    22021 tttgcttaca acctctcata tacgcatact aaacgtattt ggcgaacaaa tccactaaaa
    22081 aatccaagaa tcgacttctt ggatgatttt ttctttcatt aaactggtta cctagttata
    22141 tatctctagg gttgttttca tgtctgagtg cccgatatga tttatttttc gtttctataa
    22201 ttactttttg ccttccagtt tcttttaata ttgtactcaa gcaagtggga attggttctt
    22261 ttgtcatcca tgtatgcaaa atgttataat gctggtaaac aaaaatgatt acctgtcatt
    22321 tttatttagt tcgtatttaa ttaagttgac tatctgtata ctctgtctaa aacttaagaa
    22381 ttgttgtttt cgctgtcgct acccaatttt aatgaatgtc tgtaggttaa aactgttgtc
    22441 cttgttatag atagtaaagg agagcttgaa aatgaaaact gcgattacgt ttctattaga
    22501 ggaatatgcc gattgggaag gtgcttatat tactagcaca ctaaaccaaa atgagaaatg
    22561 gcaaaacaaa actgcttcta tccaatcaga ggtcacttcc attggaggat taaaaacaaa
    22621 aatcgattac ttaatcgaac aaatacctga aaaagttgac ctttttatta tgatcggtgg
    22681 caattcatgg gacataaaaa atcatcattt atatcaaata atcgataaat tgttaaaaac
    22741 agacgtacca gtagctgcta tttgtggggc agttgattat ttagctcaga gcggattatt
    22801 gcaaggttac aaacacaccg gaaatgcgca atttccttgg ttagaatcgg aaaattatca
    22861 gaataaagaa gattttatag aacaacaagc tgtgaaagat cgtaatttga ttactgcgaa
    22921 tggaacagct cctatagaat tcacagactt gattcttcaa gcagtccatt ttgaaacaga
    22981 agaaataaga gaaaaaacga ttcgtctata caaacttgga ttctatgatt actataaaaa
    23041 gtacggagat cctttctcct aatcattctc ttcaattaaa tactccttat caatagtgtg
    23101 acaagtaatt ttactgatga ttatttgtca cactttttct gacatttatc aaagaagata
    23161 cactctattt atattcatag acaaggtttt acggattcaa aaattcagga ttatgctata
    23221 ctggtattaa aaaaataatg aggtatttta tatgaaaaag ttaggtttta ttttattgtt
    23281 ggtctcttta ttccttgtag gatgtacgtc tcctagtaaa gaaaatagtg atccatctgc
    23341 gaccacagac aaaagttcaa tcagacaaac cagttcagct gaaaaatcaa gtactcagaa
    23401 atcagcttct actgaaacga atacatcttc aacaagcagt aaagtctcag agcccaatac
    23461 ttcttctaca tctagtagta cagttgagac aaatatttct tcttcaacaa tagacacaac
    23521 ctcctcacag tcttctcagt caatagatcc gttagcagga tattctgatc tacaaattga
    23581 gtacgcacgt gtttggctag ctattagagg taatactttt agagatacat cgggtgattt
    23641 cgaattacat gttattcatt atcctgcagg aacacctatc gccccttatg atgcagggag
    23701 tgcagtctat ccgaaagatg tggttatgct cactgggaaa tacagttacc agggagtttt
    23761 agtgtattca agtaaccatg acgggacaat cactagttat ccagttcctt cacattggca
    23821 aatgcctgca gatcaaacat cttatccagc gttcattaga gaaacaactc aagaaatcat
    23881 tgataatgca tctgtagtag ctattcctac aggaaatccc aatgacgtga aacagttaat
    23941 cgatgtgaca gttatagata attagacttt ctaatatgtt atacaaaaag aagctgacag
    24001 cattttgtca gcttctttca ctttcttttc tatttagatt tattggtctt tgatagactc
    24061 caaggcagga aaaaattttt taaataaacg tttgcaaaac agtcgctttt ctatcttcta
    24121 ccgctacttc tgcataggca ccattaatca atgtcagttg tggcggtaca tgaccacaat
    24181 caatatcata caagatcggt agttttaatt cctttgacag atcttcgtat acatccttca
    24241 ttttataatc taacaaatcc tctgccgcac ttctaccgaa catgatcccc gagctgccat
    24301 caaaccagcc tgccagtttc atctgaacaa gtgatcttct taaatcaact gacgatagct
    24361 cacagttttc tagaaaccac aagatcggct ttccatcatt atactttctt ttaaaagatt
    24421 ggacatctcc ataaggagta ccgattaaat gcctgatcgt atcaatacaa ccacctaata
    24481 atcgtccctt catccttact gcatctccgc tgactgattg ccaatttgtt ggttcagtga
    24541 gatggaaaac agaagatgat gaatgctcat ggggccacac tttttgatac ttttcagaag
    24601 attgctgtag aatcgattct ccttctcttg ttttcagtac actttcccac attgctgtcg
    24661 tttcatccaa gtactctcct cttaaatcaa taaggttagt tccatgtgca gtggctatcc
    24721 ctgtttttaa tgtgacagca agtagcaacg tactaatatc tgaataacct actatccatt
    24781 ttggttgtag agcatcaaaa tcgacaaaat cgagaatctc aatcaataat tctcctcccc
    24841 aagtcggaaa gatcatttgg atattttcat cttgcatcat ttgattgaat tcatctgctc
    24901 gttttcttgc tggacctgat ttttccttat cttgcgtcca aagcgtcttt ccagtaacta
    24961 cttggaaaga tttttcttcc attctcttta aagctgattc gagtaacgaa tggtgttcct
    25021 ctgatacgcc tgaggatggt gcagtaaccc caatcgttgc atttctttct aataatggat
    25081 aacttatcat gttgcttcac ctccaagtat gcactatttt acttggttaa ttgttttttt
    25141 cgtttgccgg catcaaaaat atcggcatct caagtagctg gatgattttg tttaagtcaa
    25201 ccgtttggat gaccatcgtt tgatctggtc tacctggtgc aaaaacaagt ttttcatttg
    25261 ataagagctc ttcatcaata ataatcggaa tctgtttttc ataaccaaac ggagaaacag
    25321 cgccctgctt ttgtcccgtt tgttcttcca tgcgttctga tgaaacaata gaaagccttt
    25381 taccgacgat cttccctaat gctttgaaat ccgtttgttt cgtagtaaaa gtcagatata
    25441 cgtagttatt atctttttta tcttttaaga ataagctttt ggtctctgta cctgtaaatc
    25501 cttgttcttt ctttattacc aaagcatctt catttgtata aatggctcga tgatgataca
    25561 actcaaaata tatattttcg tgtaagaatt tctttgccaa gtcaatcatt tttatgtctc
    25621 ttttctattt tattatttga gacatcttat catataaata tagcggttac aacattactt
    25681 gtgttttgtt cgttttttat acataactat tgtttttctg gtaaaataaa tcaaaccact
    25741 taatggaggc gttttagaat ggaaaaattt cagccgatca tcaatcaaat caaagaaaaa
    25801 gaacatcaac aaaagatcaa agaccttttt gctaagataa cagaaacgta tccccagcta
    25861 gacacaactg taaaatggaa ccaacctatg tttactgacc atggtacctt tattattgca
    25921 tttagttttg ctaaaaatca tttttctatc gcaccggaaa aagcagcgat tcgtgccctt
    25981 gaaaaaaata tccaagaagc aggctacgta tatacggata acgtcatcaa agttccttgg
    26041 aaaagtgtaa tcaactggga attgattgaa caattgattt catttaatat tgaagataaa
    26101 aaaggtcatg ataaattttg gcgataaagc tttatacgaa taaacctttt actggcgagg
    26161 aaactattca tcaccttata caggttttgt tcaatcctat cctttttatc atccctggtt
    26221 aaacacttaa ttgacaaagt atctgctatt tttataagga gtctttcttg tgctgaaaaa
    26281 aatcactgat tgaatggctt tgaattttct acatagtaaa attatttttt cagcaatttt
    26341 cctgcaaaac ttttgaataa ggaaaaaata cgagaaaata tcagtgatct ttcataatac
    26401 tgcccattat tttcaggtat atttaaatat aatatcactt taggagagaa cgaatttctt
    26461 tagagaaata aaagttagaa ttttctgact tgatttttac aatgacctta attaaaatcg
    26521 ttctttctac atttcgtgat taagcaaata tcaactaagt cagtagaacc attcacatag
    26581 gagggtgaac attgaaagat ttttactcag taaatgaatt agccgaacaa ttaggtgtta
    26641 ctacacgttc tatcagaaat tatcttcatg aaggaaaact aaaaggaaca aaggttggcg
    26701 ggcaatggaa attttctgaa cgtaatcttt ttgagttcct atacggagat caagcagagg
    26761 aagcagcaaa agaaatgcag cgattcatgc taaatgcacc aatcacgatg cgtttcaacc
    26821 ttcaatatcg tgattttaca gcaatcaatc aattccgaga acaattagtc cagtaccata
    26881 acgatgttta cgcaaataaa aaagaccgtc ttttacaata cgatctttat aaagacaatc
    26941 atgcggagat actaatcggt ggtaacttca attatgtcgt aaactttagt caatggatca
    27001 atgaaaaatt attgatccag acagatattt cccttgtatc ttaaacaatt aaaaagcgct
    27061 agaaattcca gagatgttgg aaattctagc gctatttttt tataagtact tactgtttgg
    27121 ttcaaacaaa tattttttta ataaatgaaa ttttaccatc tttatcctca aataaagacc
    27181 gtgaccatgc ttcctactac gatcagcaat aatccgataa tcgttaattt tttttctttt
    27241 ggtgtcttat tttcatgcag gatgtacata ccgcctaatg tagagataac tacgtttagc
    27301 tgtgtaatga tatatgcagt agcaacccca ttttcatttg ctgaaaaaat ataagccaat
    27361 gctgacacac taaagatggc tccaacgatc gttgctttcc aactcttgtc ttcagaaaat
    27421 gcggttggtt tacggaaaat cacataaatc actgccccta aaaagacacc gatcatttgc
    27481 ggaaagaaaa tggaaagtcc gcttgcatca actagtttag gcaatgcact gtatacccaa
    27541 taaccaatac ttgttgatgc taatagacta attccttttg tgataccgcc tttttttcca
    27601 ggatcatcac tgattgccgt taaagcagaa ccaaatacaa gaagaatgat tgctgctgct
    27661 ccaatgattt tccccatact tccagaccat tctccaaaag cgaagacacc aatcaacgag
    27721 gttccgacta actgtaatgc agtcgaaatt ggcatcgttt tagaaacacc aatcaagttg
    27781 aaagatgtat actgtcctgt ttgtccaata acccaaaatg caccagaaag taaactaatc
    27841 aaaaatgtag tcactgaaat accagctgga gaagcgaata attgaacgag taagccgaca
    27901 agcaccgctc caataccagt tcctaatatc tcatttgttg gtctgccacc aatctttttc
    27961 aaaatcagtg gctggatccc ccagccaact gccgggatca atgcaatcaa taaacccatt
    28021 tttattcacc cttatccttt tccaccttgg aaagaaggat ataaagtcat cccaccatct
    28081 gcaaatagtg taatacctgt aacataactg gattcgtctg aagccaacca tgcagctgtc
    28141 gccgctattt cttttggttc acctacgcgt cctagtggga tcatactttc caagagttct
    28201 ttttgttcag gatcatcaaa ttttgccgca ttaataggtg tacggatagc accagggcct
    28261 atactattga ttctgattcc cttcggtgca tactccattg ctaccgtttc ggtaaataat
    28321 tttaccccgc cttttgaagc agcgtaatga gcaaacgttg gccatgggat ctgttcatga
    28381 acagaagaca tattgatgat atttcctttt ttattattct ctgtaaagta atttaaagcc
    28441 gctttcgttc cgataaaaac gccagtcaaa ttgactttta acactctttc ccaatcttca
    28501 aggctaagtt catgggttgg atgctgattt tccatcccag cattattaac ccaaacgtcc
    28561 agtgtcccaa aattttctaa agccgtatca agtaaacgct gtattccttc ttcagaactg
    28621 acatctgctt gtacagagac tgcttttccg cctgcctctt cgattgcttt gactgctttt
    28681 tctgctcctt ctttatccga gttatagttg acaactacat tcattttttc tttcccgaat
    28741 cgttgagcaa tcgctgttcc aatccccttt gagccaccag ttactactgc gactttgttt
    28801 tctaattcct tgtacatagt acccactcct ttttcatacc taaacctgtt attacggata
    28861 acttttattt ccgccttaaa gtgagtgtac accaagatat atagtctaaa gcaaatgata
    28921 cgctctagca atcgttttca tgaattgcta gtgtcctttt atttctataa agaaagaggt
    28981 tgtgacagaa gtggtcagct tcaagaaatt tcggcttatt tccgaagaag ctacttctga
    29041 aaaaccattt atttggaaaa aaaggctgtg acaagacttt tgtcacagcc cctttcctgg
    29101 gcttatttat cacccatttt attgtaattg ctagttatac ataaacaaga gattttatgc
    29161 ataatgtttt attgctgaga aaaatattca gctgttgctt gttcatcctc cttgagttgt
    29221 tcaatcaatt cctctacact agaaaatttc acttgatctc ttaaaaattg attccagcgt
    29281 accgcgacat gttcaccata gatatcttga ttaaagtcca aaatatacaa ctctacagtc
    29341 agctcccgtc cttcaccgaa tgtatcatta tgtccaattg aacccatggc tggataccat
    29401 gtttccccaa tttttatttc cgacacataa acaccttctt taggcaaatg aaccgtgctc
    29461 tttactttga catttgctgt agggaatcct agtagtctgc ctcgtgcatc gccatggaca
    29521 actacccctt ctacctcata tgtgcaacct aatagttttg ctgcttcttt tacttgtccg
    29581 ctgtctagta atccacgaat acgggtagag ctgatttttt caccttgttc ttcttcttta
    29641 gaaactgtga cgattttgaa tcttcctttg gcatattcag gaaaatgagc gacatctgca
    29701 atgtcttttg gtccatacgt ataatcaaaa ccggaaacag catattttgc atgtaaccct
    29761 acgatgtatt gatcaacaaa ttcctgcggt gctaaatgag caaaagaaga agtaaaatca
    29821 acttcatata aataatctat acccaaagcg gccatttttt cttctttttg cttaaggcta
    29881 gtcaaatatt tcacgctgct aggatcgatc tttttaaaga caatagatgg atgctgattg
    29941 aaagtcatca atgctaattt caatccttct ttttgtgcaa tttccatacc tgtctttatg
    30001 actttctggt gtccgatatg cacgccgtcg aaaaatccca gcactaaaac gacatcgtct
    30061 tctggaattt gttctgattg gtaaggatgt ctgatcttga tgatattcat tcgttattcc
    30121 tcatttctca atactttact aggttttaat ttgttttttt ctttcgggtt tggctgataa
    30181 atactgacaa cttttccttt ataaaacaaa gcaatttctt ctgacggcat ttctgggagc
    30241 gcaaatacag tataatcaag tctcataccg ttttttactt tctgccatac ttcatcagta
    30301 atatctactc gtttgaaccg ttccactcct ttttctattg gcagcaaaca atcatcgatt
    30361 gtcccatttt tcacagcttg agctacttct tctaatgtca gagcatcctt ctgggacata
    30421 ccggcacttg ctgttcgggt cagatcagac atgtgcgcag ggtaaccaag tttcttgcct
    30481 gtatctacag ctaatgtgcg gacataggtt cctttcccgc attctacttc gaagcgccaa
    30541 gaaatggttc ctgtcacttc gtctcttgtc aattcacttg tccggataaa acggtaaatc
    30601 tgagcctttc taacaggacg ctccactgtt tcattattgc gggcatattc atataaccgc
    30661 ttaccgttga cttttaccgc tgagtacatt ggtggtatct gggtgatctc accaataaat
    30721 gaagccatca cttcatctat ttttttttca gataaagaca tatctactgg ttttgtttct
    30781 acccgttctc cggatcgatc ttccgtcgtt gtactgaaac ctagagttat ctcgcctaca
    30841 tagattttcc cagaatctgt caaatattcg ataacttttg tggcttttcc aatacaaatg
    30901 ggcaacacac cgtctacgtc tgggtcaagc gtacccccat gtccgatttt tttcgtatgt
    30961 aagatttttc ttaatttgaa tacgcaatca tgacttgtca ttccgcgttc tttccataaa
    31021 ggtaataacc cgtccatgtc ttttctccat ttcatttttg atttaactga attattataa
    31081 cacattgcat caattactga cgaatttgac atcataatga acggttttta tttttcggca
    31141 ataaaaacgt aacaaaattc atgctcacgt aaatagaaat catgttatgc ttttttaaag
    31201 gagggaatag aatggaagat tttttctttg caccatttga ttggatttat gggtggctta
    31261 caggtaaata gtttggtttt cacttcccaa aaacgaaaaa aaagactggc cattcttcca
    31321 gtcttttttc cgtttttttt atttattttt tcagtttctt aatcaatgta aaaaattcta
    31381 ttttgcggga tcgattttat cattttattg gtcaagattt ttccatttcc aatccaatac
    31441 ttctggcaga tcaataccat tttcacgaat atagttttga tgttcttgta atttctgatc
    31501 catttttttc gcaaaatctt ctgcttgact tcctttgatt tttaaagctg cgtcttttgc
    31561 caaatgataa cgatcaagtt catttactac acgcatgtca aatggtgtcg tgatatctcc
    31621 attttctttg tagccatgaa tcattaaatg atggttatga cgatcaaaga aaatcgtgcg
    31681 gaccaatgtt tcataaccgt ggaaagcaaa taagatcggt ttgtcttttg taaaatattg
    31741 atcaaattct tcatcgctca acccacgcgg atcttgactt ggatgacgta atttcaataa
    31801 atctacgaca ttgatgaacc gaatttttag ttttgggaat tcttccagca gcaaagttac
    31861 agctgctaat gcttctaagt tagattccgt tcctgcagca gcaatgacca attcaggttc
    31921 ctcctctttt gctgtcgaag cccaatcaat gattttcaag ccttcattca ctaagacggc
    31981 tgcttcttct gctgagtaaa attgagcacg cggatgtttg gaagagatga tcaaattgat
    32041 tttttctgac gagcggaatg ctttatccat gactgcgagc aatgtatttg catccgcagg
    32101 cagatattca cggatgaact ccgccttctt ttctgccaaa tgtgtcaata ttcctggatc
    32161 ttggtggcta tatccattgt gatcttgctg gaagactgtt gagcttgcaa tgagatttag
    32221 cgaaggataa tcatttcgcc aagaaaggtc atgtgatttc cgcatccatt tgaagtgctg
    32281 agtcaacata gagtcaacca ctcgcaagaa tgattcgtaa cttgcaaaaa accatgccgt
    32341 ccagtcagaa cgtacccttc taagaaccct tcatcttggt gttccgataa ttgactgtcg
    32401 atgacgcgac cagatggcga aagccattca tcattttctg gttcgatttt ttccatccat
    32461 tggcgatttg tcgttttgaa aacttgattc aatcggtttg attttgtttc atctggtcca
    32521 aatatacgga aattttctgg atttttctca atgatctcgc gaacgaattt accaagttcg
    32581 gtcatatctt ctgctttaac ggagcctggt ttttcaaatt gcaaagtata ttttttccaa
    32641 tctggcataa taagcggtct tggatcgatt ccaccattgg tgataggatt ttttgccatt
    32701 ctttggtcac cagtaggcgc gatttctctg atttcttcta aaatacgtcc ttgtgcatca
    32761 aataattttt ctggttcata ggatttcagc caacggagta aagcatctgc atgatccata
    32821 tgttcttgat ctacaggaat cggtacttgg tgtgctcgga aggttccttc aattggttcc
    32881 ccgtcccatt cttttggacc ggtccatcct tttggtgtgc gtacgatcaa tactggccat
    32941 tttggcatca cgacatctgt tgctgtcttc aggcgagctt ctttttggat ggatttaatt
    33001 ttctcgattg cttgatccat tttttcagcc ataacaggat ttaatttttc tgaatcattt
    33061 ccttcaataa agaaaggttc ccagccaagt ccgttaaagt aatttgctaa ttctccatct
    33121 gatttacgtg ccaagatcgt tgggttggct atttttgcac catttaaatg aaggatcggt
    33181 aaaacagctc catctatcac tggattgata aagacatttg agaaccagct tccagctaaa
    33241 ggaccggttt ctgcttcacc atcaccaatc acgacagcag agattacttc aggattgtct
    33301 aatacagctc cgactgcatg tgaaagagag tatccaagct ctccgccttc atggatagaa
    33361 cctggtgttt ctggtgctgc atgagaagca atgcctccag gaaatgaaaa tcgtttgaat
    33421 aatttttgca ttcctgtctc atcttccgtt acttctggat agatctctgt gtaacttcca
    33481 tctaaataag cattcgatac catgacttgt ccgccgtgtc ctggcccttc aatataaaac
    33541 atgttcaaat catatttatt gatgactcgg ttcaaatgga cataaataaa attttggcca
    33601 ggaatcgttc cccaatgacc gattggatgt tttttgacat cttcgggttt cagtgtacgt
    33661 ctcagtaagg ggttatcttt aagataaagt tgcccaacag aaagataatt agcagcacgc
    33721 caccaagccg tcattttatc aaagtattct ttactagaat aatccataca taacactcct
    33781 tttcattatt actcttattc tagcgcagat actcccaatt gaaacctaat aagcacttct
    33841 gttcatttgt ttggatttta tatctagcca aatgaacagg tataaaaaac cacgaaattt
    33901 ttcctttcgc tgacgttttt tacattgatc caaaaaactt gtcgttatca caatggctgt
    33961 tccgcagttc atttcgctcc tcatcatgca taatttgctg aatgacgctg ggccagttaa
    34021 cacattgctg gaaaactggg gctggatcga gcaattcctt tcctgattga tccgttttat
    34081 gtaaaagtct ctgttatctt tgtcaatatg tgaattggta tcccagctac tatgctaatt
    34141 tctacaggga ttatccaaaa tttggatcgt gaccaaatcg aagcggcgta atcggaaata
    34201 tcaataactt caacgtgatt tttcttttaa ctgtcaacgg accgacaaac tcaaactttt
    34261 atggtgcagg ttccactgat cttctagtta cttggctata cctgactgtc actaaaatag
    34321 attacaatct agcttccgtt atcggtatct tgatctttat ctatatgcaa aaatttatat
    34381 actactagaa aaaagaaagt cgaatttttt acttttcttt ttctcttttt tggttagctg
    34441 acacgccgca agactaaagt catggaactc ttaggtaata cgtcttaatg aaaacaaaaa
    34501 aatcccgcaa cccctgattt cacaggattt gcgggagttt ttgattagtc tttgttcaag
    34561 tcgcgaagca attcatcgat atggttgcca tagacaactg attcatcaag ttcaaagaac
    34621 aattcaggtg tcttataaat acttagattg tgccctaact ctcgacgaat caatcctgat
    34681 gctttattca atccatcttg tgctgttttc ttatctgatg ctaagtcaga taaaatgctg
    34741 taatagatcg ttgcttgctg taagtccccc gtaactttca catctgtgat cgttacgttc
    34801 tccacacgag ggtcacggac ctttttgcgc aaaatatcat tgacttcttt taagatttct
    34861 tgaccgactc ggcggtcacg gtagttagcc ataatgattg gcctcctttt atcagttttt
    34921 agtcattttt gacttcttcc atgataaatc cttcaattac gtcatctact ttgatgtcat
    34981 tgaatttttc aatcattgca ccacattcat aacccatttt gacttctttc acgtcatctt
    35041 tgaatcgttt cagacttgca agttggcctt catagattac gattccgtca cggattacac
    35101 ggacaccgct atctctacga atatatcctt ctgttacata ggcacctgca atcgtaccaa
    35161 ctttagaaac tttgaaggtt tcacgaacga tcatttgacc ggtgattttt tcttcaaatt
    35221 cagggtctag catccctttc atcgcagttt cgatttcttc gatggcttta tagatgatac
    35281 ggtgaagacg aatatcgact tcttcagtat cagcttgcac ttttgcctgc ggtgtaggac
    35341 gaacgttgaa accgatgata atagcgttgc tggctgctgc taaggttaca tcactttcat
    35401 tgattgctcc gacagctgaa tggacgattt ttacacggac accttccaca tcaatcttct
    35461 tcaatgatgc agccagtgct tctgctgaac cttgtacatc tgctttgatg attacgttaa
    35521 cttctttcaa ttcgccttct ttcaggcttt caaataagtt atccaaagtc acacggcttg
    35581 tggcagcacg gttttctaat aatgcacgtt tcgcgcgttc ttcacccgct gcacgtgctg
    35641 tcttttcgtc ttcgaagact acgaaacggt cacctgcttg aggtacatca ttcaagccag
    35701 tgatttctac tggcgttgca ggtcctgctt ctttatcgcg acggccaaga tcgtttgtca
    35761 ttacacggac acgtccgtaa gtattaccga caacgattgg gtcaccaaca tgaagtgttc
    35821 cttcttggac aagtaaggtt gacacaggac ctttaccttt atcaagacgt gcttcgatca
    35881 ctgttccgat tgcgcgttgt gttggatctg cttttaaatc ttctacttct gcgactaaca
    35941 ggatcatttc cagcaattct tcgatgtttt ggccgaattt cgctgagatc tcaacaaaga
    36001 tcgtttctcc accccatgct tcaggaatta actcatactc acttaattct tgcataacat
    36061 gttgagggtt tgcacctggt ttatcaattt tatttacagc gacgatgatt ggaacgcctg
    36121 cagctttggc atggttgatt gcttcaactg tttgtggcat gacgccatca tctgcagcaa
    36181 caactaagat cgtgatatct gtgatacttg ctccacgtgc acgcatactt gtaaaggccg
    36241 catgtcctgg tgtatccaag aaagtgattg gtttaccatc aatatcgatc tggtaggcac
    36301 cgatatgctg tgtgattccg ccggcttcgc cgcttgttac acgtgaatgg cgtaatgtat
    36361 ccaataaagt tgttttccca tggtctacgt gtcccataat cgttacgact ggcggacgtg
    36421 agactaattt atctggatcg acttcttctg cctcgaagaa tttatcgata tcagcaatgt
    36481 caacttggac tttttcttgt ggttccatgc catagtccgt cgctaacagt tcaatcgtat
    36541 ctttatccag tgcttggttt tgattgacca tgacaccaag cataaataat tttttaatga
    36601 tttctgctgg ctcacgatga atttttttcg caatatctgc tacgttcata ccttcagtat
    36661 actctaatac atctggcaat tcacggaatt tgcgtggtgg tacagctggt ttagttgatt
    36721 gttgctgttt gcctttttta ccttttttat tataacgatt acggttatta ttattaaatt
    36781 tgccgcgatt gttattgttt ggtttgttgt tattgttagt acggttttga ttgccgcctt
    36841 ggctcgttcc ttgacgattt tggctgctgg tatttcccgt attattttgg tttgttgttg
    36901 gtttttttcc ttgattcact tgaccgctcc cttgaccgcg atcttgataa ctgcggttat
    36961 ttttattttt ttgttgattg ttcgttgttt cttttttacg ttgatcgttc ggctgttgat
    37021 tggctggttt tctgcttgct tgttgtgcag cttgttgttt cgtgcttggg tttttgaacg
    37081 cttgttgcaa tttcttctca tcattgctag taatcgcacc catatggttt ttcacatcga
    37141 tgccaagttg ttgtgctttt tccacgacgt ctttactaga ttttttgagt tcttttgcta
    37201 attcatagat tcttttgttg cccatgcaat caccttccta accttcaatt agctccttgg
    37261 tcttcttcgc aaatccggca tccattattc cgaatacctt gcgaggtttt ccgatcatct
    37321 gcgtgatttc tgcttcagaa aatagctcaa agcaaggaac ttcataatat gaactcttat
    37381 cttttatctt ttttcttgta ttctcgcttg catcggttgc cacaaacacc aattttgctt
    37441 tttgtttgcg gatttcagca atggtcagtt cttcacccgt gattagtttt cccgcgcgca
    37501 tcgctagacc gatgagattc atggcttttt gccgattaac tgtattcatt ttccaaataa
    37561 ctctttacgt gctttttggt gagtgacata gtcaagaagt tcttgataaa actcatctgt
    37621 taatgttgtt tcgagtactc gatcgagaat ttttttatcc caagcttgtt gtacttcttc
    37681 cggctcaagt gagacatacg ctcctcgtcc aggcattttt ccagttggat cgatcgatac
    37741 ttccccttct tttgaacggg tgattcggac catctctttt ttaggtttca tttcacctga
    37801 aaccacggat ttgcgtaaag gaattttcct ttgtttcatc tctttgcctc cttactaaag
    37861 ttaagcttct ggtttttctt cgaccgtttc attgttgaat gcaattgttt catattcatc
    37921 gtctgttaaa tctgactgaa cgattgcttc atcatgcagt tcttctgttt ctttttgttg
    37981 ttcttgattt tcataaaatt ccgtcatgtc agattctgat ttgatatcga ttttatggtt
    38041 agtcaattta gcagctaaac gtgcgttttg tccgcgttta ccgattgcta gcgataattg
    38101 gtagtctgga acgacaactg tgcaggcctt tggatttgtc tcatcgaaga tcacatccat
    38161 gacttgtgca ggattcaatg catttgcaat gaatacagct ggatcttcat tccattcgac
    38221 gatatccatg ttttcgcctt ttaattcatt gacgattgct tgaactcgtt gtccctttgg
    38281 cccgacacat gtaccgactg catcgatatt tggatcattt gagcgaactg ccacttttga
    38341 acgatcgcca gcttcacggg cgatgctgac aatttcaacg attccatcat agacttctgg
    38401 tacttcttgc tcaaataagc ggcgcaatag gtctggatgg ctgcggctaa caaatacttg
    38461 cggaccttta gacgtatttt ctactcggga aacataaact ttgattcgat catgtggttg
    38521 ataaaattca tttggcattt gatcttgttt agaaagaact gcctcaattt taccaagatt
    38581 gacataaata taacgtttat cctggcgttc gacgattcct tgcatgatgt ctttttcata
    38641 agcactgaat tcgttataaa taatcgttct ttctgcttca cgtacacgct gtaaaataac
    38701 ttgtttagcc gtttgcgcag cgatccgccc aaaatctttt ggtgttactt caaaacggat
    38761 cgtatcaccg atctcatatg ctggattgat taaaagtgca tctttcaatg agacttccaa
    38821 ctgtgaatcc atcacttctt ccgttacttc ttttaccgca tagacatgga tctttccttt
    38881 tttctgatca aattccactt cgacgttttg tgcttggcca tagtgacgtt tgtatgcaga
    38941 aaccaatgct gcttctaaag catcgatcac gatttctttt gagatccctt tttcagcttc
    39001 taaagcgtct aacgcattta acatttcttt actcattttt tctgattcct ctccgtgatt
    39061 aaaattggat agctaagcga gcttttgcaa tattttttcg gtcaaaagtc aattcttttt
    39121 ctcgtgtttt tatgcgtatt ttcaatgtaa gctgttcagc atcaaatgat tgaagaaatc
    39181 cttcatattg tttttcgcca tctactggtt ggtataagga gacatggaca taatcaccaa
    39241 gtgccttttc atagtcgctt tcttttttca atggccgctc tgctcccggg gaggatacct
    39301 ccaggaaata agcttgagga atcggatctg gatctgctgc atcaagtttt tcactcagtt
    39361 tctcactgac aaaggcgcat tcttcgatat cgataccgcc ttctttgtca ataaatactc
    39421 gtaagaacca gctttttcct tcttttacaa attcgacttc gacaagttca aaattttgtt
    39481 catctaaaat cggtgtcacc atttctgtga ctgtttcaac gacgctactc aaaagtttcg
    39541 cctccttaaa aaattaagtg cgtaattcca ttaaaaagag tgagcgaaaa atccgctcac
    39601 tcacagttag tatcattacc tataagaact ataccatact ctgcttaaaa ttacaagcag
    39661 acaggaaaac agatgcttat agcatatcga ataacgataa ttgattttca tctggtagat
    39721 ctttcaatac gccattgtcg ttcatatatt cgatcaatgt ttttgaaact ttaccgcggg
    39781 tggcaagatc ttctttggat aaaaatggac cgtcttttct tgcttcaacg atttgttttg
    39841 ccacgttggt tcccaaactt gggacagctc taaatggtgc gatcaacgta tcaccttcaa
    39901 taacaaagtt aacggcatcc gatttataca aatcaatcat tccaaatttg aatcctcgtt
    39961 ccagcatttc attggctaac tccagcactg tcagttgatt cttttctttt acggaagcat
    40021 ctaatccttt gtccgtgatt tctttcattg cttgtttcac agcatctttt cctttgcaca
    40081 tggagactag atcaaagtcg tctgcacgta cagaaaagta tgcacagtaa tacaaaatag
    40141 ggaaataaac cttgaaataa gcgacacgaa gagccatcaa tacgtaagct gcagcatggg
    40201 ctttcggaaa catatatttg atttttgaac aagaatcgat ataccagtcg ggcacattat
    40261 tttctttcat cgccgataga taggtttctc gcaattcatc agggatcttg ttccattgtc
    40321 ctttacgaac agtttccatg attttaaatg ccatcccgct atctaatcca gcatggatca
    40381 aataaaccat aatatcatca cgacagccga ttacttccgc cagtgtcgca tctcctcgtc
    40441 gaatcaattc ttccgcattt cctaaccata cgtctgtacc atgagatagt ccggaaattt
    40501 gcagtagttc tgcaaaagtt gtaggatgcg tttcttctag catccctcga acaaaacgag
    40561 tgccgaattc tggaatacct aatgtccctg ttttggaata aatttgttcc tgacttactc
    40621 caagtacttc aggaccggca aatatccgca tcacttccgg gtcatccgta gggatcgttt
    40681 gtggatcgat ccctgataaa tcttgaagca tccgaataac agttgggtca tcatgcccta
    40741 gaatatctaa tttcaataca ttatcgtgaa tcgagtgaaa atcaaaatga gtcgttttcc
    40801 attccgaatt ttgatcgtct gctggatatt ggatcggcgt aaaatcatag acatccatat
    40861 aatcgggaat aacgatgatc ccccctggat gctggccggt tgtccgtttg actccagtag
    40921 cgccttttgc taatctgtcg acttctgcac ttcgaaactg cagattattg tccctttcgt
    40981 atccttttac atagccatat gctgttttgt cggcgactgt accaatcgtt ccggcacggt
    41041 aaacatagtc ttcgccaaac aatactttcg tatagttatg ggcttctgct tgataatcac
    41101 ctgagaagtt caaatcaata tcgggcactt tgtctccatg gaatcctaaa aatgtctcaa
    41161 atggaatatc atgaccgtct ttgtttaagc gtgcaccaca ttttggacat tgtttttctg
    41221 gcatgtcaaa tccagaacca tacgtaccat cttcaaaaaa ctcggaatat tggcactctg
    41281 gacaataata atgaggggct aacggattaa cttcagtgat tcctgtcatc gtcgcaacaa
    41341 agctagagcc aacagagcca cgtgaaccta ctagatagcc atcttcatta cttttatgca
    41401 ctagtttttg ggcgatcaga taaataaccg agaagccatt accattaata gaattcaact
    41461 ctttttttaa tcgtttttga atgatctcag gtaacggatc tccgtacatt tgttttgctt
    41521 ttgtgtaact caattctgat atttcatctt ctgaacctgg aatcttcggt gtataaagtt
    41581 catctttgac tggaatcact tcttcgcaga tatctgcgat tttattcgta ttttctacta
    41641 cgatctcttt cgctgtttct tcccctaaaa agtgaaatgc agttagcatc tcatcggttg
    41701 ttcggaaatg gacatccggc aaactatgac gattcaacgg attcgctccg cccatagaat
    41761 tgatcaaaat ttttcggtaa atggcatctt cttcgttcag ataatgtaca ttacctgttg
    41821 caacaacgat tttatccaaa ctttttccga tttctaccaa attttggata atttcttcca
    41881 aatcatgttc attttttacc aattcttgct cgatcaaagg cgcatagacc gcttttggca
    41941 taacttcaat ataatcgtag aattttgctc ggtttcttgc ctcttctacc cctttttgca
    42001 tcatggcttc gaatatttct cccttatcac aagctgagcc gatcaataga ttttcacgca
    42061 tctttttcaa ttgggagcgt ggtattcgtg ggacgcgttc gaagtattcg acattcgaca
    42121 ttgaaatcag tttgaacaaa tctttcagtc ctgcctgatt ttttgctaaa atcgttacat
    42181 ggaaaggtcg agcccttttg taagaatctc cttctccgat atgctcattt agctgatcat
    42241 gataaagcat gttatgattg tccattgcct ctttcacaaa gatccatgct aagtgaccag
    42301 ttgcttcagc atcatagatt gctcggtgat gctgttccaa gctgacacca aattttttag
    42361 ataacacacc taatccgaaa cgtttgaatt gaggatacag atatctagct aattccaacg
    42421 tatcaatgac tggattggcc gcttcaggaa taccatacct tgcgtagcta gtattcaaga
    42481 accccatatc aaaagccgcg ttgtgggcta ccaaaattgt atcttttgaa aattcaagga
    42541 acatccgcaa tacttcttct tccgactttg atcctcgaac cattccatcc gtgataccag
    42601 tcagatctac cgttgttcgt gacaaaggat gtcctggatc gataaattca tcaaagcttt
    42661 cgatcacatt tcctttatac attttgactg cggctagctc gatgatcgtg tcatatactg
    42721 cagacaatcc tgtcgtttcc acgtcaaata cgacatatgt ggcttcactc aatgcttcat
    42781 gtgcatcatt ataagcaatt gggacaccat catctacgac attcgcttcc acgccgtata
    42841 agattttcac acctgctttt tttccagcac tatgtgcttc cggaaaggct tgcgcaccac
    42901 cgtggtctgt aatggcgata gctcggtgtc cccatttccc tgcttgtgcc acaagatcag
    42961 agatgctgtt tgttgcatcc atcgtactca tattgctatg aacatgaagc tctactcgtt
    43021 tttccccttc aggagcataa tctttgcgag gtgtatgttt gacttctatg atatcttgcg
    43081 cattcatgac caaatcacgg acaaacgtat cttcttgaat acttcctcgt acctttaacc
    43141 agctaccgac actgatcgca tcaaatactt gttcatcttt ttcgccgttc gagaattttt
    43201 tgacgataaa tgatgaagta taatctgtga ttttcaaagt caagatcttg cgttttgaac
    43261 gtagttctct gacttctttg tcgaatacgt acccctcaag tgtgactctt cgttcttctt
    43321 caacgatact gatcattggt gtcgttggtt catcggctgg aatatttcgt cctagctgga
    43381 tgtggatggg tccttcaaga gctggcgatt gctcttttct ttcctttttc ttttgttcgt
    43441 ggacaactag actttcagct gcttgcttca taaaggcttc tgcctgttct tgtttgcgtt
    43501 cttcaaaaag tttcaatact cgttccgctt gctgctcatc cacttctggt tcgatacgaa
    43561 attttgggaa tccgtaactg acgaacaact cttctactaa tggcaagtat tgttgcttca
    43621 aataagaaat agctcctgta ctgtcgattg gcaagatgac tttacgatct tttatcacag
    43681 gtacctgcgt tttcaatact tgctgtgcta gtggtgtatc gcattgctga ttttccaaag
    43741 cttgggccca gtaatcttgc aagagctgtt catcgaatgt ctggtcatct gctaagatat
    43801 tcagtttgac ttgtgcaata tctttaaaag cgatagtcag atgctgagtc aatgaccgat
    43861 atagcataat aggcaaaatt ttggaaaaat gcagcgtaaa ttcccataaa cgcgatttac
    43921 gatggaccac tacactatcc atttcaccag cagtaagcag tggatgattt ttttctgctt
    43981 cctctagttg gatttgttcc aacagttttt caaataaatc tcgtttttct gacaaagata
    44041 tacgcccttt ctgatgagaa acaatgcaat tgatcgcatc ttatcctctt ctttttcagt
    44101 accttttagt atactcgttt tttctgagtt ttacattaaa aaattggaac tgcgattcaa
    44161 atcgcagttc caattttaaa agcgaatcat cagattgtca aaaacagctg attcagctat
    44221 ttttattttc tgtgttttaa cgcttattat tcgccttggt tcaagaggat tggtaaagta
    44281 ttcaccaatt cttctttacg tacttcaacc atttcgccag ttttcttgat tttcacttct
    44341 acgattccat ccactgcttt tttaccgaca gtgattctga ttggacagcc aatcaaatca
    44401 gaatccgcga atttcactcc tgcgcgttca tttctgtcat caaccagtac ttcatagcca
    44461 gcttttgtca tcgcttcttc tagttcattc gtcaggtttg tttggtattc gtcttttaga
    44521 ttcatttgga cgatatgcaa atcaaatggc gcaattcctt ttggccagtt gattccttgt
    44581 tcatctgaat gttgttctac gattgctgat aataaacgac ttacaccgat accataacag
    44641 cccataatca caaatttttc tcgtccattt tcgtctagta cagttgctcc catagattca
    44701 ctgtaacgag tgccgatttt gaagatatgt ccgatttcga ttcctcgagt aaaggcaaga
    44761 acaccattgt tgtctggtga aggatctcct tcttgtacaa aacgaagatc ttcataagaa
    44821 agtacagtga aatcgcgttc tggattcaca tttttatagt ggaatcctgt ttcgttcgct
    44881 ccagtaatgg cattcgctaa atcttggaca taacggtctg catatacttt tacgtcatct
    44941 gaaacaccga ctggtccgac agaaccaaac tcagcaccta gatatttttg tgcttcttct
    45001 tctgttgctt cttctaaaaa gtcagcagat aaaaagtttt tcagttttac gtcattgact
    45061 tcatggtctc cgcgtaccaa aacaaggaca ggttgttcat ccgcaatgaa caatacagac
    45121 ttgatgattt tttgaggttc aacagaaaag aaatctgcta cttcgtcaat tgattttacg
    45181 ttaggtgtag ctactttctc caattcaagc tgtgtttcat gcgatttttt tgatacatag
    45241 tagcttgtag ccatctccaa gtttgcagca taatcacttt cagtcgaata acagatcgta
    45301 tcctccccga tttctgagat cgccatgaat tcttttgagt ctttcccgcc cattgcgcca
    45361 ccgtctccga taatcgagcg aaattctaag ccacaacgct tgaatacttc ggtgtaggct
    45421 ttttcgtaat ctttatatgt ctcgtccaaa ctttcttccg ttgcatggaa agaatacgca
    45481 tctttcatga taaactctct tccgcgaagc aaaccgaagc gaggtcgttt ttcgtcacga
    45541 tatttaggtt ggatttgata aagatttaaa ggtaatttct tatatgaatt gatttcatta
    45601 cgcatcaaat cagtaaaagt ttcttcgtga gttggtccaa ggatcatttt tcgatcattg
    45661 cggtctttga aacgatacaa gtttggtcca tatgtttcgt aacgcccgga ttcttcccaa
    45721 agttctgctg gcaagatggc aggcatcagc atttctaccg ctccgatttt ttcaaattct
    45781 tcccgcataa tgcgtttcag tttttccaat acacgatttg ctaatggcag atatgaataa
    45841 atacccgctg atacttggcg gatatatcca gctcttagca agatttgatg gcttaatact
    45901 tctgcatcat ttggaacttc gcgtaatgtt ggaatcaaca tttttgattg tctcattcta
    45961 tatactcctt tatttcctca aaatagttat cgtttgttat tataccctat ttgtcaactt
    46021 tttctagaaa aagaatcgtt gaatatcgtt ccatgtaact agcaccatca acaacattaa
    46081 aaatccaaag ccagctaatg tcaatattcc ttctttttct tgacttaatg gtttcccgcg
    46141 aatcccttca aaaatattca agactaattt cccgccatct aaggcaggaa tcggaagcag
    46201 attgacgatt ccgagattca ttgaaagaag cgccatcaaa ctaataacag ttgttacacc
    46261 ttgatttgct gcttctgaag acaattggta catcatgaca ggtccgccta atttatctaa
    46321 actgaaacct gtaaatagtg atcctaaggc tttgaaaatt tccaatgaac cgctgaaagc
    46381 ttgacgcgtc cctccaatga tcttatccat aaaacctgta ttcattgggg cttttatacc
    46441 aagttggcca actttttcgc catttgactc tacagatttc ggtgtcacgt cgacagattg
    46501 cacctgacct tctcgttcga tcttaaagtc taatgtttta tccggatttt tcgtaatgac
    46561 tgttgtcaga tcattccatg aatggatttc ttttccgtcc acactgacta cctcatcatt
    46621 ttcttttaag cctgcttctg ccgcagctcc attaggcatg atctctccta cgcgattagt
    46681 gttcgtcact tgaacaccac cttgcataaa tgccagaatg atgaataaca ggattgccaa
    46741 aataaagtta ttcatgggtc cagcaaagtt ggtcagcata cgctgccaca atttagccga
    46801 ttggaactgt acgtcttttg gcgcaattct gatttctgtt ccgtcgtttt caataatcgt
    46861 cgcatcgtga tcaacagggt aggttactac ttctgtttcg tctccattca cataaccagt
    46921 gatagtcagc tcatcttcta aatcataacg gctcaattcc atcgggatag catttgtcaa
    46981 ttggattttc ttacttaaat ttatcttctc tacgatgccg tcagaattta gtaaaagaga
    47041 aagaggcatt cctggtgcca tttctgtttc atcatcccca ttgcctgcca ttcgaacata
    47101 accgccaata ggtaataggc gaagcgtata tgttgtcccg tcttttgctt gatgcccata
    47161 aattttcgga cccataccga tagcaaattc gcgaacgagg attcctgatc gtttcgcaaa
    47221 gaagaaatga ccaaactcat gaacaatcac taatatacca aaaacgatga taaatgtcag
    47281 aatcgttttc atagcttttt tctcctttac ttgttttttg tgttttttat aaaacaacga
    47341 taaaacacta taattttacc atagtatacc aaagacacca atagtatcaa tataatgaaa
    47401 aataaaaacc tgcgtctttt atgaaattca caaagaaacg caggtatact tactaaataa
    47461 tgccaaataa atgcatgata ggaaatacaa acagcaaact gtcgaaacgg tctaagattc
    47521 caccatggcc tggtaagata tttcccgagt ctttcacatc ataatgacgt ttgattgcag
    47581 attcgaccaa atcaccaaat tgtccgactg ctgaaaaaat aatcgtcatc agaagcatga
    47641 caaacatacc atgattaaat agttcttttg ctgggtatag caataaataa agaaatgcga
    47701 ctacaacagc actaccaata ccaccgagcg caccttcaat cgttttgttg ggcgaaatat
    47761 caggacttaa cttatgctga ccataacgtc gaccaaccat atatgctccg atatcagttg
    47821 cccaaacaat aaatagacca aatatcaaaa cagccaagct ttctgttcgt gcattgacaa
    47881 aattctggaa acctattccc acatacaagc tgacaagcac aggaaaacct gcttcatcaa
    47941 tcgtatacgt atttttagaa ataactgatg ttcctaaaag aatcataact ataagataaa
    48001 ataacataaa tgtacttaca ttctctggaa gaaagaaaaa ccatctttct tttggcaaaa
    48061 caagtaacac tgcaccaacg gcggataaaa ctccttcaaa acttaagagt gtcaatcctt
    48121 tcatccgaaa cagctcgtaa actcctatta ctgctaagat tgctgctgta agctcaaccg
    48181 ctatgcctcc gatccatata atcggaataa acaagactag cgccacggct gctgtgacca
    48241 cacgttgttt catgactgat cccctttctc atcttctgtt ttttttacac cgccgaatcg
    48301 acggtctcga ttttgataag aggcgattgc ttcttcaaga tgcgcaccat caaaatcagg
    48361 ccaaagcgct tttgtaaaat acagctcact atatgcgatc tgccataaca gaaaattact
    48421 aattcgctct tctccacttg tacggatcat cagctcagga tcttgtaact ctttaggcaa
    48481 gaaacctgtc attaaatgct tagcaatcaa ttcatcatcg atttcttctg cgtgtatttc
    48541 tccttttgct acttcttcgg caatttcttg gacagcagtc acgatctctg ctctgcttcc
    48601 ataattcaaa gcaaaattca ataccattcc agtattgttt tttgtttgct caattgcacg
    48661 tcttacagca tcttgcgtat gttctggcaa tacgttttca taacccatga catgaacttt
    48721 gacattttct ttaatcaatt ctggtacgaa cgtatcaaag aagtcgacag gtagttgcat
    48781 gagaaaactt acttcatctt ttggtcgctt ccagttttca gtcgaaaagg cataaagcgt
    48841 caacactttc actccaagcc ttgatgcttt ttttgtgact ttctttactg tttccatgcc
    48901 ttctttatgg ccagcaactc gtggcaaacg acggttttgc gcccaacgac cattgccgtc
    48961 catgatgatg gcaatgtgct gaggaattgg tccatcggta ttaaattgaa attcgctttt
    49021 ttcttgaata tatttgtttt tttgcggaaa aaaacgtaac attctttttc ctcctatact
    49081 taaaagattc ccactatctt aagtattata gcaatatcta actgtttttc ctatgaaatc
    49141 attgtttttt tatttaaaaa ctgaataatt ttttcttttt ttaattttta aataaaagaa
    49201 atataaataa aaacagagga agcggaatga tccgcccctc tgtttttatg attttattaa
    49261 acttccaata attctttttc tttatcagca gcgatcttat ctacttcttt gatgctgtta
    49321 tctgtcaatg tttgtacgtc tttttctaat ccgcgcaaat catcttcggt aatatcacca
    49381 tttttttgtt gttttttata ttcatcgatc gcatcacgac ggatgttacg aatcgcgatt
    49441 tttgcatttt ctgcttcttt tttaacatct ttagctaatt cttttctgcg ttcttctgtc
    49501 agttgcggga taactaagcg aatgacattt ccatcattgg ttggactgat tccgatatca
    49561 cttgccataa tagctttttc aacatcttga agaatgctct tgtcaaaagg tgtgatcatt
    49621 aaaacacgtg cttctggaat ttggattgat gccatttggt tcaatggtgt tggcgcaccg
    49681 tagtagttca cagtaatacg gtccaaaagg ctagcgttcg cacggcctgc gcggatttgc
    49741 ccaagttcac gttgaagatt ttgtgctgct tttttcattt tttcttttgc ttctgtcata
    49801 attgcatctg ccatttttat ttccccctta cagttgttcc gatattttcg cctaagattg
    49861 cacggcgaat atttccgtgt tcatttaaat tgaagacaag cagtggaata tcattatcca
    49921 tgcttagtga acttgcagtt gaatccatga cttgtaatcc tttggcaata acttccagat
    49981 gagtcagttc ttcaaatttt actgcatttg catctacttt cggatcagca gaataaacac
    50041 cgtctacatt gtttttagcc atcaggataa catctgcgcc gatttctgcc gcgcgtaatg
    50101 ctgctgttgt gtctgtcgag aagtacgggt tgccggtacc gcctgcaaag ataacgatcc
    50161 gtcctttttc taaatgacgt tctgctttac ggcgaatata tggctctgcg atttgacgca
    50221 tttcaatcga agtttgcacg cgtgtcggta cacccacgtt ttccaacgta tcctgtaaag
    50281 ctaaggcatt cataactgta gctaacatac ccatgtaatc agcttgagca cgttccatgc
    50341 ccatttgtgc tccgatttgt ccgcgccaga tatttccgcc gcccacaacg attgccattt
    50401 caacgcccag ctcgtgaact tctttgattt cttggatgat ttctttgatt actggtggtt
    50461 tgattccaaa tcctgcgtcc ccagctaaag cttctccact caattttaag actacacgct
    50521 gatatttcgg tgtgaccatg ttcattcctc caatattttc tattgccatt ttaccatact
    50581 tagatgattt cgacactcta agactggttt ttcttgtttt gcttaaaaaa agggagcgca
    50641 caaaccgtgc gttcccgtct gttattcaac agaattattt tttcacttgg ctcataactt
    50701 cttctacaaa gttatcttcg cgtttttcga taccttcgcc aacttcgaaa cgtacgaatg
    50761 ttttaacagt tgcgcctttt gatgcaacgt atttttcaac agtcatatca ggatctttaa
    50821 cgaatggttg gtccaccaaa gcgatttctg ctttgaattt cttcaagcgt ccttctacca
    50881 ttttttcaac gatattcgct ggtttgcctt cgttcaatgc ttgttctgta agaacagttt
    50941 tttcatgttc taattcagct tctggaattt gtgattcgtt tacataacga ggattgatag
    51001 ctgctacgtg catagctacg tctcttgcaa ctgtttcgtc agttgttcca tctagaacag
    51061 tcaatacggc aatacgtccg cccatatgca agtatccacc aaatgcagca ttgtcgtctt
    51121 tttctacaac ttcaaagcga cggaagctga ttttttctcc gataacttga gttgcttcga
    51181 tcaagtcaga ttcaattgta cctttatctg ttttgatttt cattgcagct tccatgtctg
    51241 ctggtttgtt ttcagcaaca agttctgcaa tttcttttac taaatcttgg aacatttcgt
    51301 ttttagaaac aaagtctgtt tctgagttta cttcaacgat tgctgcagtg ttacctttca
    51361 cagcaactga agccaatcct tcagctgcaa tgcggtcatt tttcttcgca gcctttgcca
    51421 ttcctttttc acgtaaaaga tcaactgctt tttccatgtc gccttctact tcaactaatg
    51481 cttttttcgc atccatcatt cctacgccag tcatttcgcg tagttctttt accattttag
    51541 ctgtaacgtc tgccatgttt aactcctcct gtgtgttttt ctttttaaaa aaagctgctt
    51601 caaaggagag tgaggcttgt cgcccagcct ttgaaacagc tccatcattc aaaattattc
    51661 tgctgaagaa ttgtcgcctt caacaacatc tacgatttct tcgattgatg ttgctgtgtt
    51721 ttcttctgtg aaatcttctt caactacttg atcttcacct tggtttcctt cgatgaaagc
    51781 atcagccatt tttgaagtaa tcaatttaac ggcacgaatt gcatcgtcgt ttgaagggat
    51841 tactacgtcg atttcatctg gatcgcagtt tgtatcaacc atagctacga ttgggatatt
    51901 taatttgtgt gcttcttgaa cagcaatacg ttctttacgt gggtcaacga tgtacattac
    51961 atctgggatt cttggcatat cagcgatacc gcctaagaat ttttcaagac gttcacgttc
    52021 tttgttcaaa ccagcaactt cttttttagg tagaacttca aaagttccat cttcttccat
    52081 tgcattgatt tgtttcaaac ggctgatacg tttttggata gtatcccagt ttgtcaatgt
    52141 tccgcctaac caacgatggt taacgaagta ttgtcctgca cgagttgctt cttctttgat
    52201 tgcttcttgt gcttgttttt ttgtacctac gaataatgct acgccgcctt cttcagcaac
    52261 gctcttcatg taatcataag ctgcatctac taatttaact gttttttgca agtcaatgat
    52321 gtagattccg tttctttctg tgaagatata tttcttcatt tttggattcc agcgacgagt
    52381 ttggtgacca aagtgtacgc cggcttctag taattgtttc atagaaatta ctgccatgtt
    52441 tgtttcctcc gatttggttt tatttttcct cttcttatct tctaccttgc cggcaaactt
    52501 tttcaagcac ctaccgacaa tccaataaga atgtggattt gctgatgcat aaagcaccct
    52561 cagctattat acagacaaaa gatccgtatc gcaagagcaa atatactttt ctgctcaaca
    52621 ttcaatagtt tacctgaaag ataaaaagaa ttcaagaaaa acagtagatt tctctttgtt
    52681 tctttattat cctttttcca taaaataatt ttaggtataa aagacgaata ttattgggca
    52741 atccttcttt tttttgttat gatacatgtg aaatctttcg taaaggagaa agaaaatgcg
    52801 caaagctcaa agacatttgc tgattaaaca agtcattgaa gaattcccga tccgtacgca
    52861 agaagaacta ttaaacaaac ttgggcatta tggcgttacc gccacgcagg caacgatttc
    52921 tcgagatatc cgtgatctaa aaatcgtgaa ggcacctgat gaaaatggcg tttcccgctt
    52981 cgtattgttt cgcgaaaaag gaactcctga gactaggact gaggaagagg atcggctggt
    53041 tcaaatgatt gaagacattg tattgaaagt cgaacgagtc cactttttga cgatcgtcaa
    53101 taccttgcca gacaatgctc atttatttgc atctgtttta gatgagatca aacctccctc
    53161 tattgtcagt acaattgctg gattcgatac gatcatggtc atctcggaaa atgaggagga
    53221 cgcaaaacga gtagaagaat ttcttcacca tccaactgaa gtatcacttt tttaatcaga
    53281 gcacgtattc ctaatatgga atgcgtgttt ttttattttc aatattttcc ttattgaata
    53341 aacctttaca ctctttacaa aaatacatca ttttttattc aaatttttca acaccttgat
    53401 tgaatcacaa taatcatcat ttatattgga tgttaacaag agcagcttct tgctctttgt
    53461 gtatttcaaa aaagaaatga aaggaaatca tatgtataaa ttattaactt ctggaccatt
    53521 gacgacttct gaaacagtga aaaatgctat gctgatcgat cattgtactt gggatcaaga
    53581 atacaaagaa attacacaat ggataagaaa aaaattattg gatcttgctc aagtatcgga
    53641 aggaatatac acaacggtct taatgcaagg tagcagctcc ttcggggtag aatcggtatt
    53701 aagttctgtg atcaggccag aagatactgt actgatttgt tcaaacggcg catatagaca
    53761 acgaatgatc aacatgtgcg aatgtctgaa actgaactat accgtttatg aggtatcgga
    53821 acatcaaatc ctccaagctc atgtgattac acagctgatt gaagagaatc cgtctatctc
    53881 cgcactagca atggtccata gcgagactac ttccggtatt ttgaataatt tggaagagat
    53941 tgctgctgtc acacaagaaa gagatctttt gtttatagtg gacgcgatgt caagctttgg
    54001 tgggataccg atttgtgtag ccgaactagg gattgatttt ttgatttcca gctcaaacaa
    54061 atgtattcaa ggtgttccag gcttttcttt tgtacttgct aataaagcgg aactggaaaa
    54121 acgaaaaaat gttagccgaa gtttgtcact tgatctatat gaccagtacc gcgtgatgga
    54181 aaacgagaat ggcaaatggc gtttcacttc ccctacccat acggttttgg cttttcaaca
    54241 agcgctgaag gaactagagg cagaaggagg aatcgaaact cgatattcac gttatctcgc
    54301 caataatcat gtgctacgac aaaaaatgac agagatgggg ttccagcctt tcattcaaga
    54361 atatcagggg ccattcatca cctcttttct ttaccctaag gactggcgat ttgattttaa
    54421 tcatttttat cagtatttaa agcaaaatgg atttgtgatc tatcccggga aaatcagcga
    54481 tcatgaatgt tttcgtatcg gaaatattgg cgagatctat ccggaagata ttttggaact
    54541 gacagcaatt atcaaagact ataaggagaa cgttaatgat taaaactgta atttttgatt
    54601 gggcaggaac aactgtggac tttggctgta tggctcctgt ccacgctttt agaaatgctt
    54661 ttctagaaaa aggaatccaa ctgactgaca aagaaatccg ctaaccgatg ggaaaattaa
    54721 aatgggatca tatccaacag cttctttccc tcccttctgt caaaaaacaa tggatagcta
    54781 tttatggaca actacctcag gaaaaagagg tggaggaatt gtatcaacaa tttgaacatt
    54841 atctgtttaa agatttggtc aagcattcta ccttgaaacc cgacacattg gaaacgatcg
    54901 aacggttgag aaacgctggg atcaatattg gttccacgaa tggatttaat gctcctatga
    54961 tgacaatcgt agcagaaacc gcaaaaaaag aaaggtattc accagacttc atggcaactt
    55021 ttgaagacgt cgaagggtat ggtcgaccgt ttccttatat gatccataaa aacatgcagt
    55081 acttcaaaaa caagtcggtt gatgaagtaa tcaaagtcgg agatacggtc tcagatatcc
    55141 aggaagggaa aaatgccgga gtgttgacag taggtgtaat tgaaggtagt tcgctaatgg
    55201 ggatttctta tgatgagtac caaaaattag agatggggaa aaagatcgtt taaaacagtc
    55261 tgttgctctt agattccaac aagccggcgc tgatatttgt gtaaacaatt taaaagagtt
    55321 aacagaatac attttgtacg ctgacatgaa tccagaatga cttttataca aaataagagg
    55381 ttgtgaaaaa gctgtcctgt atcaagtaat aaaaacagcc aagcaaaaat agctcctcat
    55441 atttttcgtt tggttaggct tattaccgca gatgcaagct tttgaacacc gtttattcga
    55501 aaaaaaaaag ggagctgtga cacgactttt gtcacagtcc ccttatttgc aaacaaacag
    55561 tgtttaagag acaacctctc cactaaacgg tgttcattca gctgactctc ggtatttgct
    55621 caaaaaacga taaaatttga aaagctattt tctcttttgt ttctctaata ctcggatcag
    55681 aacgctgatt tcacattctc tttttcttac atcttatctg gtgcatgaag tcctagcaaa
    55741 cgaaggtctt ctttcaataa tacagtcact gcataaacta gagctaagcg tgcttctttt
    55801 tgttcatcat cagctaaaat cttcgtatga gcatagtatt tgttgaatgc ttgtgctaat
    55861 ttgattgcat gtttcgcaat cactgacggt tcgtattttt ccccagcaga caagaccgtt
    55921 tcagggtatt tttgaactaa tttaaccact tcccagctgg tatcgtcatt caatgcatag
    55981 tctgctgttt ctgaagggac aaatcctgct ttttccaaca ggctgactgc acgagcatgt
    56041 gtatattgca cataaggtcc agtttctcct tcaaatcgaa caacttcttc caggttaaag
    56101 tcaaatgtat tcagtcgatc gtttttcaaa tcgtggaaaa tgactgcccc aaccccaact
    56161 tgtttagcta cagcatcttt gttttccaaa tcagggtttt tctcgctgat ttgttcttta
    56221 gcagattgga ttgcttcatt taatacttct tctaataaga cgatctttcc tttacgagtt
    56281 gataattttt taccaccttg cgtgatcaaa ccaaatggaa tatggtgcat gtcgtcagac
    56341 cagtcaaagc ccatttcctt caagacagct ttcaactgtt taaaatgata gctttgttca
    56401 ttccctacta catatagaga ttgtttgaag tcgtaagtac gtttacggta caatgcagca
    56461 gctaagtcac gtgtaatgta tagtgtcgca ccgtctgact tcttaatcaa cgctggattc
    56521 aggtcataag cagataggtc aacgatctct gcacctttat cctcgttcag caaatgtttt
    56581 tcttctaaaa gtttgacgat ttcatccatc ttgtcattgt agaaagcttc accgttcaac
    56641 gaatcgaaac gtacttccag cagatcatag attttattga attctttcat cgattcatca
    56701 cggaaccatt gccataattg aatagcctct ttatcgcctt cttccagacg tttgaaccaa
    56761 gcacgagctt cttcatttaa ttctggttct gtttcagcta cttcatggaa ttggacgtat
    56821 aaacgaagca attcattgat tggttctgct ttaacagctt cttctgtacc ccattttttg
    56881 taagcgacga tcagttttcc aaactgtgtt ccccagtcac ctaaatggtt gatgcggatt
    56941 ggctgataac cgattttttc cataatgaaa ccgatcgaat taccgataac tgtcgaacgc
    57001 aaatgtccca ttgagattgg tttggcaata ttaggagaag acatgtcgat tggtactgtt
    57061 ccttgattcc cgatgttgct atccccataa tgttcttttt ctttcactac tgtttgcaaa
    57121 acttttttgc tgataagttc tttattcatg aaaaagttca agtaaggtcc gacaacttcg
    57181 attttttcaa agttggcaga atcgattttt tctgctaaat ctgcagcgat ttgctgtggc
    57241 gcttttcggt aaacttttgc taatgaaaag gctggaaatg caacatctcc gtgttcagct
    57301 gattttggat tttctagtaa ttgctcgact tgttccagtg ttaaatcatc tttgacagcg
    57361 tcatagacag cttttgcgac aagatcttta ttattcatct ttattcctcc taaagtgttc
    57421 ccttgattgt taaacaaaaa aaatctctag cgacggcata cttagtatgc catcactaga
    57481 gacgagattt cccgcggtac cactccaatt gtcctaaaaa aggaccagct ccatctgata
    57541 tcggtagacg accgtctgtt taaaacagaa ttctccaagt gcgcttcgtt gtatcttctt
    57601 cattcggctt ccaccacttc cgaattcgct agccaagaat gatacaagta ctctcttgtt
    57661 caacaaatct tagtcttatt aaactttctt gaattatatc agaaaaaaaa acgaaaggct
    57721 agtcgtttca taaaaaaaca atccagatgg cctaaaggag aagaaatacc acctggatag
    57781 ctataaatca tttcgtaaat acgaaagact taacaaatta atcagctacg ataatcgttc
    57841 cttcttcaga agctaaaaga ttctcgatat tttctaaaga agtgatgatt gccttagcgt
    57901 ttggttttgc ttcaacaaat gcaatagctg cttctacttt cggaagcatg cttcctggag
    57961 cgaattgctt ttcatcaatg tattgtttca tttctgaaac agtaacagtt tctaatttct
    58021 tttgatctgg tttttggtaa ttgacataca cattgtctac tcctgtcaat acgatcagca
    58081 aatccgcatc gatgatttcg gctaattttt ctgaagcaaa atctttatcg attacggctt
    58141 ctcgtccttc taatccagta gctgtttcta caacaggaat cccaccgcca ccaacagaga
    58201 ctgtgataac tccttgatcg accagcgttt cgatgacacg tgcttcttta atcgagatgg
    58261 gtttagggga agcgactact tttctccatc cacgtccagc atcttctttg aatgtcacag
    58321 tggagtcagc attcatttgt tctttggctt cttcttccgt gtagaatgga ccgactggtt
    58381 tacttggatt tttgaatgaa ggatcatttt catccacaat aacttgagtg actaaagaaa
    58441 ccacatcttt atcgatccct ttttccttca gtacttcgcc catggcgttt tgtaaccagt
    58501 aaccgataga tccttctgtc attgcaacac atgtatctaa aggcattgcc ggtgttttct
    58561 cagaatcagc agcttgctgc tgaatcaata ggttaccgac ttgcggacca tttccgtggg
    58621 agatgatcaa ttcatctcct tgttcgataa atttcactaa atactttgct gtttccatta
    58681 acgcttcttg ttgggctttt gcacttgcat ccgtagacaa gatggcattg ccccctaaag
    58741 cgacaactac ttttcgtttg gccatttcat tcgtcctttc ttcagttcta ccgaattttc
    58801 tagataacaa aacgagccag cgttttgtga ttttccctca atgctgactc gttttttcgt
    58861 gagatgaatg tggttatgct taaacgcgag ggataaacaa gttacctaat gttgctgcca
    58921 tgatcgcttt gattgagtgc atacgatttt ctgcttggtc aaattgacga gcatatttgc
    58981 tgcggaagac ttcatctgtt acttccattt cagtgatgcc aaagcgttct ttcatttgtt
    59041 caccgtaaac agtgtttgta tcatggaaag ctggcaagca gtgcaagaag atcaaacgat
    59101 ctgtgttgtg tgttttttct accatttcca tattgatttg gtaaggtttc aacaatttga
    59161 tacgttcttc gaatttatct tcttcaccca ttgataccca tacatcagaa taaaggacgt
    59221 ctgctccgtc tacaccttta tcaacatcgt cggtaatcat caattgtgca ccggattttt
    59281 cagcaaagcc ttctgccatt ttgacgattt cttcttcagg ttgtaattct tttggtgcaa
    59341 cgatacgcat attcgctcct aaaattgcac ctgtgactaa taaagagtta gccatattgt
    59401 ttcgaccatc tccgcagtaa gcaacagtga taccttctac tgttccaaag ttttcttgga
    59461 tagttaaaaa gtcagcgatc atttgtgttg gatgccattc atcagtcaag ccattccaaa
    59521 ctggaacacc agagaattct gctagttcct ctaccatttt ttgactgaat ccacggaatt
    59581 cgattccgtc aaacatacgt cctagtactt tagctgtatc ttctgtggat tcttttttac
    59641 ctaattggat atcatttgca cctagatatt caggatgtgc acctagatcg atcgctgcag
    59701 tagtaaatgc agaacgagta cgtgtagatg ttttttcaaa taaaagcgca atattttttc
    59761 cttctagata gtgatgtggg ataccacgtt ttttcaagtc tttcaagtgt tctgaaaaat
    59821 cgatcaaata ttgtaattct tcttttgtaa agtctttttc tgctaataag cttcttcctt
    59881 ggaatactga ttctttcata ttaaacattc tcctttagat tgtttttatt taagattaaa
    59941 gaggaaatcc cgacggcagc tgacagtaag actgccgtca ggaaacaaat tacttcaagt
    60001 cttcacggac aagtggttgg ctcatgcagc gtggaccacc acgacctcgt gacaattcac
    60061 ttgaattgat ttctaacact ttgatgccat agctacgaag caattcgttc gatacataat
    60121 ttcggttata agtgactaca acacctggag cgattgccaa tgtgtttgat ccatcattcc
    60181 attgttctcg aggtgcaacg atctcatctc cattaccagt tgggatcagt acaagatcat
    60241 ctaatccaag agcagctttc atcgtttcgt gtaagttatc tgaatgagta atcttgatat
    60301 catcaccatc tggttcgatt gtaaagacat ccatcttacc gtctttactt tgaattgctg
    60361 gatggatagt gaatttatcg tgatctacca ttgtaaatac ggtatccaaa tgcatcattg
    60421 cacggttgtt cggaattttg attgccaaca ctttttcaaa tcctgaattt tttgcgaata
    60481 ggtggcgtgc taatttttct aaagctttcg cattagttcg ttgagaaatc ccaacagcta
    60541 ctactttatc gcttaagatc aattcgtctc cgccttcgat atgatctgga ttttcacgat
    60601 ccaaccatac ttcgattcct ttatttgcaa atcttggatg atgtttcaaa atatattctg
    60661 taaacatcga ttcacgacga cgtgcttcaa aagtcatctt attgactgtc atcccatttc
    60721 ccattgatgc ggaagggtcg cgtgtaaagt acaagtttgg cattgggtcc atgtaaaatg
    60781 gatagtcatc gtctgctgac agatcatata gactgctaga acgtacttcg atatcttttg
    60841 tacgaacccc agccatgatt ttatctacca tttcttgcgt ttccattgat agaaggaact
    60901 catgaagacc ctctcttaca gcattcgatg caatatgaga ttcatccaac attttattca
    60961 agaattgttc tttgacattg cctgcgtcaa tcgcttctgc cgctaatttt tccaagtaaa
    61021 gagtctcgac gccttcattt tgcaatgtct tcgcaaagtt gtcatgttct tcttgagcaa
    61081 tcggaagata tggaatatca tcgaacaaca gacgatccat aatatctggt gtcaaatttt
    61141 ccacttcttg tccaggccgt ttcagtaaaa ctgtctttaa tttccctatt tcagagaaaa
    61201 cgtgaatagg tttatccatg ttgtgttgcc tccttctttt tgattacatg tataggatat
    61261 cctccttttg taatcacttt cataattttg tctcattcaa aatg
//
LOCUS       DAJSAW010000011        60192 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJSAW010000011 DAJSAW010000000
VERSION     DAJSAW010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.011"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="ONT64_000761"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="ONT64_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR2302198.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ONT64_000762"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ONT64_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR2302199.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="ONT64_000763"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="ONT64_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR2302200.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1366..1491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1642..2274
                     /locus_tag="ONT64_000764"
     CDS             1642..2274
                     /locus_tag="ONT64_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR2302201.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="ONT64_000765"
     CDS             complement(2276..3181)
                     /locus_tag="ONT64_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR2302202.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3925)
                     /locus_tag="ONT64_000766"
     CDS             complement(3266..3925)
                     /locus_tag="ONT64_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR2302203.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="ONT64_000767"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="ONT64_000767"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCR2302204.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="ONT64_000768"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="ONT64_000768"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCR2302205.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="ONT64_000769"
     CDS             6168..7253
                     /locus_tag="ONT64_000769"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCR2302206.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="ONT64_000770"
     CDS             7250..8314
                     /locus_tag="ONT64_000770"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR2302207.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="ONT64_000771"
     CDS             complement(8563..9366)
                     /locus_tag="ONT64_000771"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCR2302208.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ONT64_000772"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ONT64_000772"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR2302209.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="ONT64_000773"
     CDS             complement(10244..11251)
                     /locus_tag="ONT64_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR2302210.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="ONT64_000774"
     CDS             complement(11258..11902)
                     /locus_tag="ONT64_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR2302211.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="ONT64_000775"
     CDS             complement(12002..12760)
                     /locus_tag="ONT64_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCR2302212.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ONT64_000776"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ONT64_000776"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR2302213.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ONT64_000777"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ONT64_000777"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR2302214.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ONT64_000778"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ONT64_000778"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR2302215.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ONT64_000779"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ONT64_000779"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR2302216.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ONT64_000780"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ONT64_000780"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR2302217.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="ONT64_000781"
     CDS             18158..19027
                     /locus_tag="ONT64_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HCR2302218.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="ONT64_000782"
     CDS             complement(19153..20052)
                     /locus_tag="ONT64_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2302219.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="ONT64_000783"
     CDS             complement(20217..21041)
                     /locus_tag="ONT64_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR2302220.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="ONT64_000784"
     CDS             complement(21052..22347)
                     /locus_tag="ONT64_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCR2302221.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="ONT64_000785"
     CDS             complement(22363..22842)
                     /locus_tag="ONT64_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR2302222.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="ONT64_000786"
     CDS             complement(22848..23876)
                     /locus_tag="ONT64_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCR2302223.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="ONT64_000787"
     CDS             complement(23893..24999)
                     /locus_tag="ONT64_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCR2302224.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ONT64_000788"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ONT64_000788"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR2302225.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="ONT64_000789"
     CDS             complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="ONT64_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR2302226.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="ONT64_000790"
     CDS             complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="ONT64_000790"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR2302227.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29970..30668
                     /locus_tag="ONT64_000791"
     CDS             29970..30668
                     /locus_tag="ONT64_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCR2302228.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30836..33475)
                     /locus_tag="ONT64_000792"
     CDS             complement(30836..33475)
                     /locus_tag="ONT64_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR2302229.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34094..35125)
                     /gene="queA"
                     /locus_tag="ONT64_000793"
     CDS             complement(34094..35125)
                     /gene="queA"
                     /locus_tag="ONT64_000793"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR2302230.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35318..36493
                     /locus_tag="ONT64_000794"
     CDS             35318..36493
                     /locus_tag="ONT64_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR2302231.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36497..37138
                     /locus_tag="ONT64_000795"
     CDS             36497..37138
                     /locus_tag="ONT64_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2302232.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37135..38052
                     /locus_tag="ONT64_000796"
     CDS             37135..38052
                     /locus_tag="ONT64_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2302233.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38049..38720
                     /locus_tag="ONT64_000797"
     CDS             38049..38720
                     /locus_tag="ONT64_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2302234.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39261..39749)
                     /locus_tag="ONT64_000798"
     CDS             complement(39261..39749)
                     /locus_tag="ONT64_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HCR2302235.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39950..40216)
                     /locus_tag="ONT64_000799"
     CDS             complement(39950..40216)
                     /locus_tag="ONT64_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR2302236.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40216..40479)
                     /locus_tag="ONT64_000800"
     CDS             complement(40216..40479)
                     /locus_tag="ONT64_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR2302237.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            40955..41140
                     /locus_tag="ONT64_000801"
     CDS             40955..41140
                     /locus_tag="ONT64_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR2302238.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(41282..43105)
                     /locus_tag="ONT64_000802"
     CDS             complement(41282..43105)
                     /locus_tag="ONT64_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCR2302239.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(43703..46408)
                     /locus_tag="ONT64_000803"
     CDS             complement(43703..46408)
                     /locus_tag="ONT64_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCR2302240.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(46714..47661)
                     /locus_tag="ONT64_000804"
     CDS             complement(46714..47661)
                     /locus_tag="ONT64_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCR2302241.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            47851..48120
                     /gene="rpsN"
                     /locus_tag="ONT64_000805"
     CDS             47851..48120
                     /gene="rpsN"
                     /locus_tag="ONT64_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR2302242.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(48296..48658)
                     /locus_tag="ONT64_000806"
     CDS             complement(48296..48658)
                     /locus_tag="ONT64_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR2302243.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(48669..49790)
                     /locus_tag="ONT64_000807"
     CDS             complement(48669..49790)
                     /locus_tag="ONT64_000807"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR2302244.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(49806..50156)
                     /locus_tag="ONT64_000808"
     CDS             complement(49806..50156)
                     /locus_tag="ONT64_000808"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR2302245.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(50286..51797)
                     /locus_tag="ONT64_000809"
     CDS             complement(50286..51797)
                     /locus_tag="ONT64_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR2302246.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(52119..53471)
                     /locus_tag="ONT64_000810"
     CDS             complement(52119..53471)
                     /locus_tag="ONT64_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR2302247.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(53624..54700)
                     /locus_tag="ONT64_000811"
     CDS             complement(53624..54700)
                     /locus_tag="ONT64_000811"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR2302248.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            54896..56641
                     /locus_tag="ONT64_000812"
     CDS             54896..56641
                     /locus_tag="ONT64_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HCR2302249.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(56921..57358)
                     /locus_tag="ONT64_000813"
     CDS             complement(56921..57358)
                     /locus_tag="ONT64_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR2302250.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(57342..58715)
                     /locus_tag="ONT64_000814"
     CDS             complement(57342..58715)
                     /locus_tag="ONT64_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302251.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(59037..59525)
                     /locus_tag="ONT64_000815"
     CDS             complement(59037..59525)
                     /locus_tag="ONT64_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2302252.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(59547..60149)
                     /locus_tag="ONT64_000816"
     CDS             complement(59547..60149)
                     /locus_tag="ONT64_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR2302253.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        17104 a        13016 c        10989 g        19083 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    27961 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    28021 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    28081 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    28141 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    28201 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    28261 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    28321 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    28381 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    28441 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    28501 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    28561 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    28621 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    28681 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    28741 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    28801 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    28861 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    28921 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    28981 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    29041 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    29101 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    29161 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    29221 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    29281 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    29341 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    29401 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    29461 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    29521 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    29581 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    29641 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    29701 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    29761 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    29821 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    29881 tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg
    29941 gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag
    30001 caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat
    30061 tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca
    30121 tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag
    30181 gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga
    30241 cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc
    30301 gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct
    30361 ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt
    30421 ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac
    30481 tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt
    30541 tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt attggttatc
    30601 tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag
    30661 atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga
    30721 gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc
    30781 acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt
    30841 cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta
    30901 cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata
    30961 gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca
    31021 tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta
    31081 cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga
    31141 catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac
    31201 caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc
    31261 gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg
    31321 gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt
    31381 gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct
    31441 ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac
    31501 gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg
    31561 cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt
    31621 tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt
    31681 gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag
    31741 ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg
    31801 cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat
    31861 gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt
    31921 tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt
    31981 ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa
    32041 tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc
    32101 ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt
    32161 cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta
    32221 ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca acagccctta
    32281 cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg
    32341 tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat
    32401 cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt
    32461 gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg
    32521 ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg
    32581 tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag
    32641 tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg
    32701 cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag
    32761 gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca
    32821 tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata
    32881 ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt
    32941 caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga
    33001 tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta
    33061 ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt
    33121 tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca
    33181 ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt
    33241 ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa
    33301 aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt
    33361 tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg
    33421 gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat
    33481 tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt
    33541 atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc
    33601 ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca
    33661 tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta
    33721 caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat
    33781 gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt
    33841 aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa
    33901 caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt
    33961 tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga
    34021 gctcggcttt ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg
    34081 gcttttcata accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct
    34141 cttcaatcgc atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta
    34201 acatgactaa agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga
    34261 attcatagcc aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat
    34321 taaatttcgt tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt
    34381 tcccgccatt tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga
    34441 attcactatg catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta
    34501 accctacatg tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta
    34561 attctttcgt aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat
    34621 agacagtttg atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca
    34681 tttctcccaa tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc
    34741 gtccgccgtg ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga
    34801 ttcttgtccc aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt
    34861 gtgtattcgt tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata
    34921 aacgagcagg cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt
    34981 caatgatttc atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta
    35041 aacgagaatg atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa
    35101 aatcaaaatc ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt
    35161 ctatcatgtt tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt
    35221 cctaaataaa aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag
    35281 atagttcttc attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc
    35341 aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg
    35401 tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat
    35461 caaccgaatg actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga
    35521 aattgatcca gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat
    35581 gcctcacatg acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca
    35641 agaagaacgg aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat
    35701 gttagatcgc tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg
    35761 tgcccttgca gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc
    35821 aatcactcgt gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac
    35881 gatcgtattt gtaacccatg acatggatga agcgattaaa ctagcaagcc gtatcgccat
    35941 catgagtgaa ggccgtgtga tccaatttga tacaccagaa aatattttac gtcatcctgc
    36001 aaacgatttc gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc
    36061 gacaagagtc ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt
    36121 acaagaagca atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa
    36181 ttcgaatgta ttaaaaggat ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt
    36241 atccagtgtc ggcgacattt tgaataaaca tgttttttac gtaaaagaat ccgcctatct
    36301 tcgtgatacc ttgcaacgca ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga
    36361 acagaacaaa gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt
    36421 gatttgggga gaagatcccg caactactga tgttgcagag tcagctactc ctgaagaatc
    36481 taaggaggtc tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa
    36541 aggttttgaa catctttata tttcaggact tgctttgatc attggtatca tcattgctgt
    36601 accgcttggt gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac
    36661 cagtgccttg cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg
    36721 cgtaggtaaa ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg
    36781 taatacgtac attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat
    36841 ggggatgact gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat
    36901 catggctggt attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta
    36961 tatcggcgct ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga
    37021 tctcattttg gcaggtacga tccctgtaac aattttagca ctattggctg attggcttct
    37081 aggtctcttg gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa
    37141 cgtatactaa aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttccttc
    37201 cctggtcttg ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca
    37261 gaagcacaga tcttagcaag tctagttgcc ggaatggttg aacattatac agataaaaat
    37321 accgcaatca tcaacaatct ggctactaca actatcaacc atcaagcgat gatgaacgga
    37381 gatgcttcaa tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt
    37441 cctcctgaaa aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga
    37501 tacggtcaaa cgtggttccc gtcctatagt tttgaaaata cgtatgtctt tcttgttcga
    37561 aaagacaccg cacaaaaata ccatctatct aaagtcagcg atctgaaaaa tgtagcggat
    37621 gaattagtag ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc
    37681 ttccaagaaa cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt
    37741 tatgacgcag tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg
    37801 atcgccagtt atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat
    37861 gctgctccag ttgtagacaa caagatatta gctgatacgc ctcatttaaa agaagcctta
    37921 aataagttag gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat
    37981 aataatctag tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt
    38041 gaagggaagt gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc
    38101 aggaaaatgg ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg
    38161 gtgtcctctt tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta
    38221 aattagcaaa ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca
    38281 tgatctccat tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat
    38341 ttttgtattc cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca
    38401 aaaacatcct tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg
    38461 tagagttacc actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg
    38521 ccatcgggat aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta
    38581 tccgaggaac caatgcaaca gagggcacat cgattatttt agcaggtgca ttgccaactg
    38641 cattaatggc gatcatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag
    38701 ccagccgtac atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg
    38761 gtcgcaaagg cttttttagt tctgtcattt ttaaggcaat aatccactct gattcttccc
    38821 tccttatggg taaagagttg cttttcttag ctatttatcc attccgtata ctccaacttc
    38881 atactgccaa cgggttcata tctgcatcca ccgactaagt ttttttctta tccgctaagg
    38941 cattttgcca atccattttc ccatcactcg tcatggaatt cataccacat gatctctaaa
    39001 tactcgtctg cttgttcttt gaattgggca tcaattctta catcctttgg cgtggctttc
    39061 atttaagcct tttccatcag tcctcgattg ttcgatgcct tcagctgcat catcaaaact
    39121 ggtgactgcc gtatcggtaa cttcgatcat cttttataca atcgggaact tgccatcctc
    39181 atccttggct aaaaacatat ctagccattc cagcttctcg ccatgggagg tttgcggttc
    39241 ctaaaaccgg tacacgattc ttatggactg catccgtcgc aactagactt ggcgatacga
    39301 tgatccttca ccagaagagc acccccgtaa accaattgat cgatatattg ccaataagaa
    39361 aagacattgg cgtaaaagat atcgatccct cgtgatgcat tgccgttgat actgctattc
    39421 atggccgaaa gcgccacgac tttaacttct tccgtttgag tgacattcat gttcggcaat
    39481 gtcgctttgg atacccgctc tgctaaagga acgacactgg tattgtattt cgtatctgta
    39541 tcttttccaa aagaccatgc caacgaatcc tctgggaacc aataagacga ctccggttga
    39601 ttgtccattg cttttttcaa catacttttg gctgtttctc gataaacgac atttccaatt
    39661 gtcttcgcct cactttggcg ataatcgatg gtcgttgcca ctgccaacac taacaacgat
    39721 gctccaattt tactgatttt ccttgtcaca cttcttcctc cttgttttac ataaacccgt
    39781 caacattcca ttcctaaact caaacaatga cccttcaaag gtggagatat aggaacaaat
    39841 taaagaaatc atgcaataaa tccaagaagg ttggttttag aacaaaaaaa gagtctcctt
    39901 tttggggaga ctctttgtta actatctaaa aaggttggct cttctcactt cactcataat
    39961 gtgtccaggc agaataaatt tctacaactt tattttcctc atcaactttg taaaccactc
    40021 gatgctgtct attcagcctg cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa
    40081 aagactgcgt gggcatatat ggatcttcct tcaatgtctg aatcacttct tcaaattgtt
    40141 tctttaaatt cgtttgcttg atttttctta aatccacttt cgcagagttt ttgattgcga
    40201 ctgtatagtt actcatcaaa gagcttccca atcaatatca tcgatattcg taaagctact
    40261 gtcgtctttt tcgcggtctc gtactttatc taaagtacct gtttgctcaa gaaaaagggt
    40321 ttcttgaatc gctctccagt cttccagccc aatcaataca gcattgtttc cactacgatc
    40381 actaataatc tcgatttcag tatgattttc atttacctct ttcaataact gataaaaatt
    40441 tttacgagca gtgctaggat tcatcgttgt attagccatt gtctctcctc ctttttctga
    40501 tataattata gcgtacttga ttaagtacgt caaaaaaata caataaagca cttaaattca
    40561 gcaaactttt cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc
    40621 cacgctcact tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca
    40681 acgtatcatc tttagcaaag gtcgaatcca aagcctgtga gtctatgcaa caacaccaat
    40741 acacgaaggt tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg
    40801 cacgaaagat ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct
    40861 caaaaatgat tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc
    40921 ttagaccgtt gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa
    40981 atggtcactt gacgaggctg agattcctac ttatgaagac acatggcact taagagtcga
    41041 ggtaaaggaa gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa
    41101 ttatgatcaa gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt
    41161 tcatacgctg ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca
    41221 aaacagaccc tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg
    41281 tttatttctt caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc
    41341 gcaatcttaa ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta
    41401 caaattgagg aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga
    41461 tatctacata acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa
    41521 aacggacatc tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat
    41581 cttcgtctag cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca
    41641 acgcaccgat atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat
    41701 aatcatgtag ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat
    41761 catggatctt ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt
    41821 cccctaaccg atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa
    41881 tattggcaat cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc
    41941 ctgtttgaga taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt
    42001 ctgttgagcc atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc
    42061 cattggaata gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt
    42121 ttgccagatt gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta
    42181 aaatcagcgc tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt
    42241 taccaatttg agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga
    42301 taaatgtgat ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag
    42361 cggcattttt tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc
    42421 cagttaaaga ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg
    42481 gcacagccgc tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat
    42541 ataaaccaac aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata
    42601 ggaacgatgc agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg
    42661 tcacagaaat agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag
    42721 cacctgctga gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg
    42781 aaatcaagcc agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat
    42841 gaggataagc atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa
    42901 caaaagcggc aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga
    42961 caacgatttg ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca
    43021 aagcagcaaa tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta
    43081 ctaacaacct ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag
    43141 ttttatagtc gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag
    43201 tcaataaact ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa
    43261 cgctttaata cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat
    43321 aaaaggatag aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg
    43381 gaagagagat gtaaggctgc tttctaccca tcattcttac tactcgaagc atagcgcagt
    43441 tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc
    43501 aaattaaaaa cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag
    43561 cttttttaca acctcttggt acacggtgtt caatcagctg accctcggta ttagctcaaa
    43621 aaaacggcaa aatatgagga actatttttc catttttttc tctaatactc aggtcaaaac
    43681 actgatttca caacctcttt ttttaatgaa aaatatggct ctcatgcgcc gcttctttga
    43741 atttcgtcat tttttcttcg atgaacggat agattgccgt tcctacgatt ccaaagacaa
    43801 caaacaaacc gatcatgatc cagattgctt tcgtagcatt tggccaataa attcctcctg
    43861 cagattcccg taacaagttt accgcatgag taaatggcaa gaacggattg atcatttgga
    43921 agaatttccc tgagacttgg atcggatagt tcccgcctcc gccagagatc gacagtacta
    43981 agatgataat agcgatccct ttaccgacat tatcgaataa tgcagccaag acatagacga
    44041 tcatcatgaa cgctaaggcg atcaagacag caaatgacac gctgtagacc ggattttgga
    44101 catctactcc tagtaagaaa atattcccta atgtaacgat caagctttgc gcaatcgcca
    44161 ttgtcaaaaa tgtcagcata cgagcaacga attgttctcg tttcgtaaaa ttgtttcggt
    44221 ctttctttcc taaatagtat tctgtcgtag caacacttga aagaagaaca gcgccgaccc
    44281 acagacatag tgcagtataa aacggggtgc tagctgaacc gttatttgcg attggataaa
    44341 gcgcattggt ttgtagctca acaggttgtg taaagaagtc actttctttt gttgcgtcta
    44401 gtttcaataa tttgatgact tcgcctagat cgacttcttg ttcgcctttt cggatcgcgt
    44461 ctgctgcttt ttggatacca gctttgatat ttggccagtc attgttgatc aagtccgcag
    44521 cagcgttcac agctgtttcg acttctggca ttttttcatt tactgttgtc aatgtaccgg
    44581 tgatttcatt ttttagtgct ggccaatcat tttggataaa atcagcggcc aatcctaatt
    44641 tttgctgaat gatcggtaat tcattgttgt ataggtctgc cccttcattg atgccattga
    44701 cgatggtatc catatttcca ttcagcattg tattggcatc atggatttct tgtccaattg
    44761 caggcatttc tttttgatat ttttctaaaa ttgccactgc attcgttaca gttgctttcg
    44821 ttgagctgag aagcgaatca aagtcgatct ggtccgcttt gctcagtgcg ccttgtgcag
    44881 tcccaatcgt atcgatgact tggtctagta gagaagtgac agtggaactg atttgacctg
    44941 tattgatacc agcaatgatg ccattgatat tattaacaac ggcatcaatt tgatctagca
    45001 attcatcaat agccttcgtg tcttcatttt gaacagctgt gttcaaagca gataggcggt
    45061 tttgcagatt gcttagcaag ggttttaatt gatttagctg atcaataatt ggctgtaatt
    45121 tcgtatttcc agcacttgtt tgcaatttag tcaggaactc gaccagttta tcgattgcat
    45181 tttgttgttg agatattccg tcaataaagc cgttcaatac attgctgatg tttttctttt
    45241 catcatcagt cagcttacca tcttcaatag ctgttcttac agtatctatt aacgaacctg
    45301 tcgttgttgc cacttgtcca attgacttca atgtaacatc tacactgttt gtgatgcttg
    45361 gtatcgcatc tttcagctga gaggcacctg attttgtttg gtttgctaga tcacttgcct
    45421 gattttctaa atttttcaca tctggcaaga tatcttgtac ttgttggatg atcgtcaagc
    45481 cttgttttgc ttcatcgatt ccttggttca ttgtctcttg gatcgaagca aaatcgccgt
    45541 cgatctctgc tagttgctta ccggcatttt ggatctctgg gattttttct tgcaaatcaa
    45601 ggatgatttt tgcttgatct ttcaactcag gcattttgtc attcaatgca acgacttttt
    45661 cacccatctc atctacttta gggatgtaat ccacgaattc atttgcttta ttcagttttt
    45721 ccttgatttc aggtagttga ctttgtaatt cgaggacttg ttttgtgtag ccgtcgatcg
    45781 tgtccaaatt ttcatctgtt gataaaatca tatctttaac tttattgata cttactaggt
    45841 tcgtatcgat gtcgtaacct atctcattga atactttcaa taaagtcgaa ctggctgttt
    45901 tgataaattc atttgtgatt tgcgattgga tcgaacttgc tcctttatcg gtgattttcg
    45961 gtgcaatcgc attgattttt tcatttaccg tatattcgat ttttggtttc ttgatatcac
    46021 cactagtaaa actcagcaga tcttctgaga aatcctttgg caagtaaata cctgcgtaat
    46081 actttccgga acggactccg tcttctaatt gttcttttga atcaacgaat tgccatccta
    46141 attgtttatt tttatgaagc gattcgatga cttgctcccc aatggccact tctttacctt
    46201 gaaactctgc tggcttgtct gcactgtaaa cagcaattgg cagctctcca gtatttccat
    46261 atggatccca taatgctttg atgttaaacc acgcatatag cgaagggatg atcatgatcg
    46321 cgatgatcag aagtgttgca atcgggtttt tgaaaatccg tttccagtca agcaaatata
    46381 atcgaagcgt atttttgatt gactgcatag ttctccccct tcttcttttt tcagctgaat
    46441 tactttacca tgtatttcat acttttttca atcggctttt ttcaaaaaag tcgaattttt
    46501 ggacatatca cggcaattgt ctcattgtaa tctcttacat attgagaaat aattaaggat
    46561 tacagccttt actttatcat gaaataaaag ggaaagataa ttttatgcca aagtttttat
    46621 ctattatacg tatacgcaaa aaagctaaaa cattttttgt ctttgcccct acaaaaatgt
    46681 ttcagctttt ttcgtttttt attcctgtat ttcttatttt tcgaatgaca ggatgatttt
    46741 cccagcagga ggatgtcctt caactagctg atgtgcttgg atgacatgct taagtgaagc
    46801 agacagattc attgaaataa aaacatgaaa ggctcctttt tggtaggctt tgattatgcc
    46861 agcaaaggct tctgcatcta gatattcctt tcttggaaca aacgattcat aaaagccttc
    46921 ctttttttgc cgcaaatcta gatctgggag gtcatttaat gcaacatacc tgccgcctgg
    46981 tttcatgatc tggatgccgg tttctccttt gatacttcct ttcgtcgcat cgattacaag
    47041 gtctgcctga tcagcaaatt gcagacctgg atttgtttta tcataagcag caaatgctga
    47101 agctcctaat ttttttacct tttcctcatt tttactggat gcactggtta agattcggat
    47161 tcccttttca tggagaagct ggatcagact tgacccgacc gcacctgatg ctcctagtat
    47221 cattacagta tctgtcggct ggatttccgt caaatggttg attagattat aagccgtaat
    47281 tcctggcgtg accattccgg ctgcttcttc ccaactcatt ttatctggaa tttttgctac
    47341 ttttgctgat ggcaacacga cttcttctcc gtaagtcccg ccgactgcat ggacagctac
    47401 tcgatcacct acatgaacag ttgtgacatc tgaaccaatc tctgtcacaa taccagcgcc
    47461 atcattacct ggtacataag ggaatttcaa tgttcgtatc tctttcattt ctcctaaacg
    47521 caaggcaaca tcataaggat tcacactgaa tgcttttatt tctacacgaa catgtccaga
    47581 accgacttct ctgggatgag catcgatttc ttcaaaaaca tcttgtgctt ctccgtaacc
    47641 gttaattgca aatcttcgca tggtcaccat ctcccttttt ctttaagttt acgtttatgc
    47701 ctttattttg acaaaaaaaa tgtcttttcg ttttggcgca tgattcaaaa caaaatagtt
    47761 gtcttttttc gcgtaacgtg ttaaattatt tatctaaaag cgtaaagatt acgatttacg
    47821 ctaataaaaa ataattggag gaatttgttg atggctaaaa aatcaaagat tgccaaagca
    47881 aaaaaacagc aagaaacgat tgaacgctat gctgctttac gccaggaatt aaaagcgcaa
    47941 aaagattacg aaggcttgcg taaacttcct ttagatgctc gtccagaacg attgaaaaat
    48001 cgtgatttaa tcgatggacg ccctcgcgga tacatgcgta aattcgggat gtcccgtata
    48061 aaattccgtg aattagcaca taaaggtttg attccaggag taaaaaaagc cagctggtaa
    48121 tccaagtctg gctgtaatgt aaaggaggag ggcgcatgga ccactccttg gatcatcaaa
    48181 aaagatacgt tcgcttcttc tagcagaaat gctggtacta gaagcagacg tatctttttt
    48241 tacgcacagc tggtcctttt ttatatgtaa aggctgcatg gttacttcgc tatatttatt
    48301 tagtaacagg agtcagtgct tcgataccca cactaatttt caatgctcgg tcaacttctg
    48361 ccataatggg tcgatccaaa tggcagacct tttctttcaa tcttgattta tcgatcgttc
    48421 tgatctgctc caacaggata acggagtctc tttctattcc tgttccagta gctttgatcc
    48481 caatgtgagt cggaagctta ggtttagcca tttttgccgt tatggccgca acaatgatcg
    48541 tcgggctaaa atggttacct agattatttt gaataaccaa aactgggcgt gttccgcctt
    48601 gctccgatcc cacgactgga gaaagatcag caaaataaat atctcctctt tttaccatag
    48661 gatcctcctt attcttgata ttctcttggt atccgctgtc ctaatccgca agccacttca
    48721 tagtggatcg tacctaattg atctgctata tcctgcatag tgatttcttt gtctgcattt
    48781 ttgccgataa gcgtcacttt tgttccgaca ggaaattctt gaggcaatcg gatcatcagt
    48841 tgatccatac atacacgccc aatcgtttca cagtagttcc cttctaccaa tagagaaaaa
    48901 ccctgcatct ttcgtggcca gccatctgca taaccgatcg gtatcgttcc gatccactct
    48961 gcttcaggag tgatatatgt ttctccatag ccgattcctt cacctgctgg gagttttttg
    49021 acttgaatca attcagaaac cagctcaagt gctggctgca atggataaac ttccggtagt
    49081 gcgtgcccag agggattaag tccgtacatt gctactccat aacgaatcat attgccgatt
    49141 gtttcatcat gccataaagc cgttgcacta ttgctgacat gtacatatcg aggaagttcg
    49201 ggaagttttt tcagcacttc tataaagcgt tctttttgca gattccaata tgtatcatct
    49261 gcctgatctg ctgtagaaaa atgggtaaat aaaccttccc attctaatac acgactttct
    49321 ttgatgaatg cagctgcctc ttttacttct tcaggtgtac aaaatccgat acgacccatc
    49381 ccagtgtctg cttttagatg aatcgaaaga ggcgtctcta gcttactttt ctctaaaaca
    49441 gcagctgctg cttgcaacca ttcccgtgta cctgcagtta ctgataaatc atgagcaaca
    49501 gctacagtca gtgcgtcgta aggaaccata ttcaagataa ggatgggctg agtaaatcca
    49561 gcttctctca actcgactcc ttcatctaaa tttgaaacac aaaagcccga agctccacct
    49621 gcaactgctg cctcagctgt ttcaatagct ccatgaccgt atccgttcgc ttttactact
    49681 gcaaacaatt cttttccttg cggaagtctt gcaacttcat ttgctacatt ttcagttata
    49741 gcttttttat gtatcactgc tttcgttggg cggtgccaag cgacaaccat cgtttttcct
    49801 tctttctagt cttccaaaat aatttgtgca aaagctgttt catttgtatg agtaatactg
    49861 acccatacat tgccttcatg aggtgaacgc gtcacaacag gctgaccatt ttcatttttt
    49921 aaaatctcga catcttgaaa ggtaactttt ccgatcccag ttccccaagc tttagagaaa
    49981 gcctctttgc aagcgtaacg cccgcctaaa aattctactt ggcgatgaaa aggcaaggac
    50041 tggaatagtt tcatttcatc agaggtcaag attcgtgcaa tgaattttgg tttttcttcg
    50101 atcagccgag tgatcctagg aagttcgact gcatcgatcc caattccttt tatcatatgc
    50161 ttttctcttt tcaataaatt tagaacactc tcattctacc aaattataac cattttgcca
    50221 caaataagtc cacaaataac aaaaatgaaa gtttctctta aaaaagagaa actttcattt
    50281 tatgtttaat cgttatttga acggattaca aatccgcgtt ttttatcatt gtgacgttta
    50341 ccgccatctt tttggctgtt attatttttg ctataaccgc cttttgattt gtttccacga
    50401 taattaccgc cgtcacgacg attacggttg tttccgccac cacgtttgcc atttttattg
    50461 tatccttttt tctgcattgg caatgggcgt tctggtgtga tcttcactgg cactgcatca
    50521 gctggatctt ttgctgtcgt ttttagcaaa agtgcaacta aatcttgtgc agaatattct
    50581 tctaataatt tatctgctgt ttgcaggtat ttatctagac cattttctgc aagttttgtt
    50641 tcgatttgtt caactgcagc acctaattga cctttgaatg cttctttttc ggttggtgga
    50701 cgtaaagtcg tcatccgttt tttcgtcaag ttttcaatca catgcaagta gtccatttca
    50761 tttggtgtaa cgaaagtcac agacataccg ccttttccgg cacggcctgt acgtccgata
    50821 cggtgaacgt agctttctgg atcttgaggg atatcatagt tgtacacatg agtgacacct
    50881 gaaatatcta atccacgtgc agcaacgtct gtcgctacaa ggatatctaa atgaccgctc
    50941 ttaaatgaac gcaatacgct cattcgtttt tgttgagaca aatcgccatg gattccttct
    51001 gcacggtaac cgcgtgcttc taatccgcgt gccaattcgt ctacacggcg ttttgtacgt
    51061 ccgaagacga ttgttaattc aggtgtttga acatcaaaca agcgagtcat gatatcgaat
    51121 ttttcgtatt cttttgcgcg gacatagtat tgatcgatca agtcagctgt catttctttc
    51181 gctttgatct tcacatgatg tggatttttc atgaatttaa cgccaatatt cttgatagct
    51241 ggcggcatcg ttgctgagaa aagtaatgtt tgtctttgat caggaacttg tgaaatgatt
    51301 ttttcgatat cttccaagaa gcccatattc aacatttcat ctgcttcatc caaaactaat
    51361 gtttgaacgg ttccaagttt caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt
    51421 gtacccacaa cgatatgtgg acgatctttc aagccacgga tctgacggcc aatatctgct
    51481 ccgccgtaaa cggcttgtac acgtactttt ttgtcgcgcc ctaaacgata taattcttct
    51541 tgtgtttgga tagctaattc acgtgttggt gcgataacta aaccttgtaa ttcgtgacga
    51601 tctggatcga ttttttctaa cattggtaat ccaaatgcag ctgttttccc tgtacctgtc
    51661 tgtgcttgtc caataacgtc ttttccagct aatgctagtg gaatcgtttc tgcttggata
    51721 ggcgttgctt cttcaaaacc agcgcgttcg actgatttca acaattctgg tgataattct
    51781 agttctttaa atttcaaatg aatcctccta tatggttgtt aactgaggat gtagcttttt
    51841 tgctaccccg tcgatttgtt cggtcaaagt gtattgtttc tttttgacag tggccacgca
    51901 cgtttttatc cattcttagc ccctgtggca ccaccgaaaa atcactttgc gaagtttagg
    51961 cattagaaaa gccaaacggg tcgcgttcag ctttatcatt attatgacat ctggcttcaa
    52021 caagttgttt ctatggcaaa accagtccaa tttctcttca tctttcggca ataggttatt
    52081 ctaacatagt ttttcgtttt ctgcaagttt acagattttt atttcatttg ctgaagttgt
    52141 tctaccactt caaataagcc catgctgttg ctgcctttta gcataatact atctgttgct
    52201 tcaagatttt gtttgatagc atcaatcaat ttattctttt ctttttcaaa atagaacaca
    52261 ggtagttggg ggtatttttg gttcaattct tcttttagtg catgcatttg ttccccataa
    52321 aggaaaatca tggcgaattt ctcaggatcg atatgttcac tcatttgcgc atgcatattt
    52381 aaagactctt tccctaattc cagcatatct gctaacacag cgattctgcg tccttttgtt
    52441 ggcagatttt taaaagtatc taataccagt cccatggctg ttggatttgc attgtagaca
    52501 tcgctcaaaa tagctgcacc atttcctgcc gttagccatt cggttctgtt ttgagtgata
    52561 gaaacatggg cgagcccttg gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc
    52621 gtatacgcaa tcaaagcatt cgttacatta taacttccag gcagcgggat cgtaaatgtt
    52681 tctccttcta ctacaaaatc ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca
    52741 tcgccatttc caataccgaa tgttgtgact gtttgtacta atgttgccgt taaaggtgtc
    52801 agcaacggtt catctgcggg cacaacgagt aatccgtctg atacaagtcc gtcagtgatt
    52861 tccattttgg ctttagcgat tccttctcgc gacccaagat tttctatgtg tgcttcaccg
    52921 atcattgtga ttgctgcagc ttttggttga gccataagtg aaagttccgt gatttcattg
    52981 gcgtgatcca tgcccatttc caaaacgatc ttttctgtat cttggggcat gtgcaaaatc
    53041 gtgtaaggaa gaccaatatt attgttgtaa tttccttgcg tcttatatgt tttgtatttt
    53101 tcagacaaga cagacgcggt tagatctttc gtggtggttt taccattgct tcctgtaatt
    53161 gcaacgactt ctgcgcctac tttttccaag taataggttg ccaattgctg catggctttc
    53221 aaaggatctg ctacaggaat aactggaaaa ttttttggag cttgttcaac aggcaagctc
    53281 caaaatgaag cagatgctcc gtttgtgatc gcttgctgga taaattcatg tccatctcgc
    53341 gtaccagcaa gaggaacgaa taaacttcct tcggttatct tccggctgtc aaattcgaca
    53401 ttggtaatct ttgtcagtgc agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt
    53461 gttagattca ttgagatctc tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg
    53521 cgggttcgca tgactctcac ttccaagtaa atttctctat tcgtatttag aagctagaat
    53581 gaacgcggtt acgcaatcac tccagcctct tctttatttc tctttattca tttttttcaa
    53641 aaaaagattg acgctgatgg tatcgattca ttcctaactg gatcagttct tcaatcaaat
    53701 caccgtattt caagcccata ttttcccata agagtgggta catactgaac tccgtaaatc
    53761 ctggcataga gtttaattca ttcaggaata attcattttt atttgtcaaa aagaaatcgc
    53821 accggctcaa tccgcttcca cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt
    53881 tttgataaac ttcttctggc acttcggctg gaatctgcat ttcgatttta ttattgatat
    53941 attttgcttc ataatcatag aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc
    54001 gaacgtcttc atttcctaat acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa
    54061 cgattgctcg agaatcatac tgataggctg ttgctaaagc attttgcagc tcttctcggt
    54121 tttctgcttt tgtaatgccg acactagaac ccatattcgc cggtttgaca aacatcggat
    54181 aaagcaaaga accttcacat tgatcaaata cttttttagg attttctttc cattgattct
    54241 taagtactgg tacataagga acttgcggca caccagcagc ttgtaaaata tacttggtca
    54301 tgattttatc catggcacat gcactggtca atacgcctgc gccgacataa ggcatattca
    54361 atgtctctaa gaagccttgg atcgttccat cttccccgtt tggcccatgt aaaactggaa
    54421 aaacgatggc tccttcttct ttgatttcgc ccggattgat cacttttcct gtaaagcctt
    54481 cctctgtctg tccacttggg tcccatgaaa gatgcaagac atctttgctg gcaggttttt
    54541 ctgttagtaa tggacctttg acccattgtc cttcttttgt aataaatacg agttgaactt
    54601 ggtaataatt ataataaatg gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat
    54661 gctctgcgct gcgtccgcca tatagtaaag taatcttcaa aagattgtcc tcctgtattc
    54721 taaatcgttc agtgatttac tctcatattt tagcacaaaa caaaaataaa cgaataactt
    54781 atttataaaa aaacgagcca gcttctaaaa gaacgagcgt catttatttt ttgtcagcta
    54841 gattatttgt cccgtttttt ataaaaagtt atgataaaga aaaggaggga tgatgatggc
    54901 gcaaacaaat ccacgcagcc gcttggcaga aatcattcga gtgttcatca attacaatgt
    54961 agtgccaaac tttgtacagc aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact
    55021 aggccccacc ttcatcaaga taggacagat gctttctgtt cgagaagatt tgctttcttc
    55081 tgccttcact caaacattca aaacattaca agatagtgta cctagcgaca cattttcaac
    55141 ggtcaaaaag acgatcgaaa cagaactttc tctttcatta tcagatatat tcgatgattt
    55201 ttcaaaatcg ccttttgcct ctgcttctat ggggcaggct catcgtgcca agctgaagaa
    55261 cggtgacgcg gtagtcgtta agatccaaca ccctaacatc gccgaagaga tccgacttga
    55321 tcttcaacta tttgaacgag cgatcccttt gatcaaatac attcctgaaa ctagcgtagt
    55381 tgatctaaaa ggggtactcc aagaagtaaa acgttccttg atcaatgaaa tggacttctt
    55441 aaaagaaagt caaaatggcg aacaattcta tcaaaagaac aatggctgga aagagatccg
    55501 cagccccaag atttatgacg ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc
    55561 tggtaaaaac ttgaatcatt taatgaacgc cgaaaacaaa actgaaactt ttatcactgg
    55621 aattcaaaac aagcagctga aacaagaagt tgcaaagctt ttagttgaga actttatgaa
    55681 acaggtgttt gatgatggtt ttttccatgc tgatcctcat ccagggaatc tgctattcca
    55741 tgtactaaca aaagaagaac agacacaagc tagccgaaaa actgaaacag tccatgaaaa
    55801 agagttcggt tcctttgctt ttcgagcctc cacttctgca gaagaccctg ttgcgccata
    55861 taccatcaac tacatcgatt ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa
    55921 actcacacaa gccgttttag cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt
    55981 ccttcgactt tgtcagcaag aaggctcgtt tgatgaaagt cggttccacc aagaactgac
    56041 aagttttctt gaacagtatt atgattctcc tattgatgaa atcaacttgc aagaagtatt
    56101 cacacatgtc gtgacgattt gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct
    56161 tttgctgaaa gcatttggga cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc
    56221 cctcatggaa gtcgcttcgc cttttgctca acattatttt ctcactcatc tggatgtaga
    56281 agatacattg aaacaaagcg gattagactt attggaaggg atgaaggctg cgcctaagat
    56341 ccctcaacag ctccaccatc tattagaaat gtggacttcg ggacaaggaa aagtcaattt
    56401 ggaattgaaa aagcaagaca aattattgag caggatcgaa agtatgatca atcggcttgt
    56461 tttcggtatg attttagctg cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc
    56521 tgtagaaaac gcagaggtcg tttccttatt agggatattt acgtatgcta ttgccgcatt
    56581 cgtcattatt tttttggcaa tcgatgcatt gatccaaatg tataagaaga ggaaaaagta
    56641 gaaaaaggtt gtgacagaaa gaatcagctt caagaaataa gaaggggttt tacataaatt
    56701 gtttctcaaa tttttgtgaa aattcggctt atttttcgaa gaggctactt ctgaaacacc
    56761 gagaataaaa aatgtgaaaa agctgttctg cacccagtaa taagaacaat caaaaaggga
    56821 acatggaaca catttatcct gtttccctgt tccctttttg atgtattcga gcataaataa
    56881 aataccttcg ttacctacta tcttctgtac ttggtaaacc ttagtcaatt gcctttttta
    56941 acgcttgacg aaaagacata tgatctttct ccgtaaaggg ttttggaccc tttgtcctta
    57001 tgccagcttt tcttaacttt ccactatgat aacgttgttc tagaagcgtg ccgatattcg
    57061 cttcgtcaaa ttcttttcca gagtgatgga acactcgcac acccttcggc aatacaagtg
    57121 aagctaaacc ataatcttgc gtgatcaaaa agtcattctt cttaaccata ccgacgattt
    57181 tataatccgc actatcagct ccgcggtcta cgtagacgat ttggacatga ttagcgtatt
    57241 ctttggtcgt ataatggtcc acactggtca cgataaccac gtcaagacca taggattctc
    57301 ctaattttat gacatcttct tttactggag aaccatctcc atcaatgaat agcctcattc
    57361 tttttcgcct tcttccataa atgataaaca gttactaatc cgctcagtaa aagaccaaca
    57421 ttgatccatt ttgccaagat aagcagttgt acactttcta tcggcactga ttcatggttc
    57481 agcgtgatca ataatagatg gattggctct cccatttctt ttgctaaaac aaacaaaagc
    57541 gtcatcaata aaaactggtg gatcacactc cctttatttt gagtagtata cttgacaacg
    57601 atccggcgat ccattggaat agctgtatca gtttgataat attccttatt tagccagctg
    57661 cctgttacta cgtgccagaa actaagcaga aagacaccta tcgcaaaacc atttggaatc
    57721 aagaggacca ataaagaaga actcagtcca agcaattgca tagccaatgg agaccatgtt
    57781 aaacgtttca aaatccatgg tccgactatt tttgctgcaa tcaagcctgc tgcataaggc
    57841 aaatacataa aaatcgaaag tttttctaat ccgtacagta tgccaacata aaaactgcta
    57901 aataaaaaaa ggaaattccc tagcattcca tttagaaaag taaacagatt tagccagctt
    57961 ggcaacttcc attccttctt ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc
    58021 aagaaaccat aaatagcaag atcaaaaagc ctttcatcga ataacaatcg ggcatttctc
    58081 aaaagaaaca ataagccaaa gaatatcaaa aataaaacaa gctctcgcgt agctaccaga
    58141 tttcttttga tgtctttgaa atccagttcg taacgcggat aatgactaac cgtatggtag
    58201 gccatgacgt aaaaaagtgt ataaatagta aagcttgctg caatctgagt cggtgcaggc
    58261 aactcaatcg cttgatacag gagaaatagc cagatagctg caaataagta ctttttccca
    58321 tccatattga taaaaccttg cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca
    58381 agccatgctg cactcagccc tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat
    58441 acgcctaata ttccaagcaa tgaaccagca ccaccaatca gcagagcgat catcagcaac
    58501 gtgtaactat ctagaccaaa tcgtagactg ttgactaaaa atagtccagt catccgaaag
    58561 gcataaaaca gcgtaaatgg caaaatcgtc agccaattat gcgagtctgt tagtcccaaa
    58621 aataaaagat aaggaataaa aataaccgtg ttacttaaga taaagggcgt tgtttgtgta
    58681 aaattacgcc caaatcgttt gagtttttcg aacatgacaa tggctcctta aacagtctta
    58741 taactaatct gctcttcgat tttagcatta aaggggaaat aaaccaattt tacagaatat
    58801 attcttgtaa aaaaaaatac agaaagaagc ataagcttct ttctgcattt ttacggtact
    58861 tgattactca agcagacaag cccttctcca caaaagtctt gcccgacacc cctgcatccg
    58921 gcaggtgata aagatagaaa tcaattatcc tgtgcctcca aaatcaatta ggagctctgt
    58981 aaaccagagt ccttctcgac ccgtcgcata aatttacttt aacacagttc ctatgtttat
    59041 gcgagagctt ttttcatttt ataatcgacc atttgaaatc cttgaccgat atctgaaata
    59101 agttcttctg taatctcaaa tccttcatgc ttataaacct cgattgcttg atgattcccc
    59161 tgattcactc gcaaaaagat ctctctttcc tttgtttgcg cagcaagttc ttcataccag
    59221 cgaaaaactg aagaagtcaa tccttgacca cgtactttat ttagcaaata taatttactt
    59281 aaataaattt ttccatttat ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt
    59341 tttaataaaa agtaggacac accttctgtc agttcttttt gaatctgttt ttttgactga
    59401 taagttttca gcatgtaatc cacttgtttt tgaccaatga tcggcacaaa aacttccggc
    59461 catacttctt gtatgactgc catcagctca tccagttcat tttctgttac ttttattttt
    59521 tccatacacc cacgtccttt tttacattat aaatgacctt gcgcttgatg gaataaaaag
    59581 agcatgatca tgctatacac acccaccgac cacggtttca agaaaatcac aatctgcata
    59641 aattccctaa aagataattt cgtcaaacca gctactagac agatcaagtc gtctggcgca
    59701 aagggaagca gcaaggtaac aactagtact tttttaatat ctttcgtttt tcttgtcagc
    59761 aattcatcaa atttctgata cttttttggc gataaaataa gctggacaaa ggctacgcca
    59821 tagtaacgga caagcaaaaa tccgattgct tccccaatca ctaggccaat acaactatat
    59881 aaaagcccaa atccattgcc aaacatcagc atgcccgcta ctgaggagat ccctcccggc
    59941 aaaataggaa tgatcacttg gatcacttgc aacagaacaa atatcatcac tgcatacttg
    60001 ccaaattgct ggataaacgc ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa
    60061 gcataaagaa tcaacacaaa gaaaaaaaac agaccaataa tgggcatgat ctgaagacat
    60121 ttttgtaaaa ctgatccttt tttagacatc tatattcccc acatcttttt tcatttatta
    60181 aaactgcctc ct
//
LOCUS       DAJSAW010000012        57915 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJSAW010000012 DAJSAW010000000
VERSION     DAJSAW010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.012"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ONT64_000817"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ONT64_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCR2302254.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ONT64_000818"
     CDS             852..1040
                     /locus_tag="ONT64_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCR2302255.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ONT64_000819"
     CDS             1053..1610
                     /locus_tag="ONT64_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR2302256.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ONT64_000820"
     CDS             1642..1884
                     /locus_tag="ONT64_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR2302257.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            2225..3439
                     /locus_tag="ONT64_000821"
     CDS             2225..3439
                     /locus_tag="ONT64_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCR2302258.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            3470..4897
                     /locus_tag="ONT64_000822"
     CDS             3470..4897
                     /locus_tag="ONT64_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2302259.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            4920..5846
                     /gene="glsA"
                     /locus_tag="ONT64_000823"
     CDS             4920..5846
                     /gene="glsA"
                     /locus_tag="ONT64_000823"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR2302260.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            6054..7514
                     /locus_tag="ONT64_000824"
     CDS             6054..7514
                     /locus_tag="ONT64_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302261.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            7735..9498
                     /locus_tag="ONT64_000825"
     CDS             7735..9498
                     /locus_tag="ONT64_000825"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR2302262.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            9582..10040
                     /locus_tag="ONT64_000826"
     CDS             9582..10040
                     /locus_tag="ONT64_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR2302263.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            10149..10832
                     /locus_tag="ONT64_000827"
     CDS             10149..10832
                     /locus_tag="ONT64_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR2302264.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            10979..11593
                     /locus_tag="ONT64_000828"
     CDS             10979..11593
                     /locus_tag="ONT64_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCR2302265.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            11609..11815
                     /locus_tag="ONT64_000829"
     CDS             11609..11815
                     /locus_tag="ONT64_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302266.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            12354..12911
                     /locus_tag="ONT64_000830"
                     /pseudo
     CDS             12354..12911
                     /locus_tag="ONT64_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            12969..15008
                     /locus_tag="ONT64_000831"
     CDS             12969..15008
                     /locus_tag="ONT64_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HCR2302267.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(15117..16268)
                     /locus_tag="ONT64_000832"
     CDS             complement(15117..16268)
                     /locus_tag="ONT64_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HCR2302268.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(16422..16952)
                     /locus_tag="ONT64_000833"
     CDS             complement(16422..16952)
                     /locus_tag="ONT64_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCR2302269.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(17051..17401)
                     /locus_tag="ONT64_000834"
     CDS             complement(17051..17401)
                     /locus_tag="ONT64_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HCR2302270.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(17558..19087)
                     /gene="ahpF"
                     /locus_tag="ONT64_000835"
     CDS             complement(17558..19087)
                     /gene="ahpF"
                     /locus_tag="ONT64_000835"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR2302271.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(19097..19660)
                     /gene="ahpC"
                     /locus_tag="ONT64_000836"
     CDS             complement(19097..19660)
                     /gene="ahpC"
                     /locus_tag="ONT64_000836"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCR2302272.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(19804..20466)
                     /locus_tag="ONT64_000837"
     CDS             complement(19804..20466)
                     /locus_tag="ONT64_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2302273.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            20650..22296
                     /locus_tag="ONT64_000838"
     CDS             20650..22296
                     /locus_tag="ONT64_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR2302274.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            22287..23369
                     /gene="uxuA"
                     /locus_tag="ONT64_000839"
     CDS             22287..23369
                     /gene="uxuA"
                     /locus_tag="ONT64_000839"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR2302275.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(23477..25030)
                     /locus_tag="ONT64_000840"
                     /pseudo
     CDS             complement(23477..25030)
                     /locus_tag="ONT64_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            25178..26319
                     /locus_tag="ONT64_000841"
                     /pseudo
     CDS             25178..26319
                     /locus_tag="ONT64_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(26445..27524)
                     /locus_tag="ONT64_000842"
     CDS             complement(26445..27524)
                     /locus_tag="ONT64_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR2302276.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(27627..28067)
                     /locus_tag="ONT64_000843"
     CDS             complement(27627..28067)
                     /locus_tag="ONT64_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2302277.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(28124..28534)
                     /locus_tag="ONT64_000844"
     CDS             complement(28124..28534)
                     /locus_tag="ONT64_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR2302278.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(28714..29148)
                     /locus_tag="ONT64_000845"
     CDS             complement(28714..29148)
                     /locus_tag="ONT64_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2302279.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(29248..30096)
                     /locus_tag="ONT64_000846"
     CDS             complement(29248..30096)
                     /locus_tag="ONT64_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2302280.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            30485..32251
                     /locus_tag="ONT64_000847"
     CDS             30485..32251
                     /locus_tag="ONT64_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HCR2302281.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            32353..33612
                     /locus_tag="ONT64_000848"
     CDS             32353..33612
                     /locus_tag="ONT64_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR2302282.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            33759..35060
                     /locus_tag="ONT64_000849"
     CDS             33759..35060
                     /locus_tag="ONT64_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2302283.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            35063..35914
                     /locus_tag="ONT64_000850"
     CDS             35063..35914
                     /locus_tag="ONT64_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2302284.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            35927..36742
                     /locus_tag="ONT64_000851"
     CDS             35927..36742
                     /locus_tag="ONT64_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HCR2302285.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            36893..38059
                     /locus_tag="ONT64_000852"
     CDS             36893..38059
                     /locus_tag="ONT64_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR2302286.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            38289..38936
                     /locus_tag="ONT64_000853"
     CDS             38289..38936
                     /locus_tag="ONT64_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCR2302287.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            39020..39964
                     /locus_tag="ONT64_000854"
     CDS             39020..39964
                     /locus_tag="ONT64_000854"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HCR2302288.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            40099..41550
                     /locus_tag="ONT64_000855"
     CDS             40099..41550
                     /locus_tag="ONT64_000855"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HCR2302289.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            41571..42407
                     /locus_tag="ONT64_000856"
     CDS             41571..42407
                     /locus_tag="ONT64_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR2302290.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            42407..43066
                     /locus_tag="ONT64_000857"
     CDS             42407..43066
                     /locus_tag="ONT64_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR2302291.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            43192..43881
                     /locus_tag="ONT64_000858"
     CDS             43192..43881
                     /locus_tag="ONT64_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302292.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            43887..44843
                     /locus_tag="ONT64_000859"
     CDS             43887..44843
                     /locus_tag="ONT64_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCR2302293.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            44887..45399
                     /locus_tag="ONT64_000860"
     CDS             44887..45399
                     /locus_tag="ONT64_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2302294.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(45655..46203)
                     /locus_tag="ONT64_000861"
     CDS             complement(45655..46203)
                     /locus_tag="ONT64_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2302295.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(46292..47473)
                     /locus_tag="ONT64_000862"
     CDS             complement(46292..47473)
                     /locus_tag="ONT64_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR2302296.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(47709..49217)
                     /locus_tag="ONT64_000863"
     CDS             complement(47709..49217)
                     /locus_tag="ONT64_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HCR2302297.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            49376..49567
                     /locus_tag="ONT64_000864"
     CDS             49376..49567
                     /locus_tag="ONT64_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302298.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            49770..50687
                     /locus_tag="ONT64_000865"
     CDS             49770..50687
                     /locus_tag="ONT64_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HCR2302299.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            50680..51753
                     /locus_tag="ONT64_000866"
     CDS             50680..51753
                     /locus_tag="ONT64_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302300.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            51901..52419
                     /locus_tag="ONT64_000867"
     CDS             51901..52419
                     /locus_tag="ONT64_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302301.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            52400..52792
                     /locus_tag="ONT64_000868"
     CDS             52400..52792
                     /locus_tag="ONT64_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HCR2302302.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            52868..53803
                     /locus_tag="ONT64_000869"
     CDS             52868..53803
                     /locus_tag="ONT64_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HCR2302303.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            53936..54715
                     /locus_tag="ONT64_000870"
     CDS             53936..54715
                     /locus_tag="ONT64_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCR2302304.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            54708..55025
                     /locus_tag="ONT64_000871"
     CDS             54708..55025
                     /locus_tag="ONT64_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCR2302305.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            55269..56879
                     /locus_tag="ONT64_000872"
     CDS             55269..56879
                     /locus_tag="ONT64_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR2302306.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            57036..57884
                     /locus_tag="ONT64_000873"
     CDS             57036..57884
                     /locus_tag="ONT64_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR2302307.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        19269 a        10229 c        11305 g        17112 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc cttatgaaaa aatatcttta aaaataaaaa aagacgaaaa taaacggttt
     2041 tctataaaaa gaagaccgtt tattttttgt ctttatttgt gtgaaaaacg aaaaagagca
     2101 caacacatca agcttttatt aattgtctgt cttttataaa tgcaagtatt aattcaaaaa
     2161 aacagaagaa aaaaacaact acaccttctt ttatactcgt aatcgtagaa aggaggcgaa
     2221 aaaaatgaat acattaccta tcaatacagg agatacagca ttcatgattt tatgtacagc
     2281 aatggtttgc ctgatgactc ctggtctagc ttttttttac ggcggattag cacggaagaa
     2341 taatatacta acgatcatgt ctcaatcttt tctctccgtt gggataacaa cgattatgtg
     2401 ggtatttggt ggattcggat tggcctttgg acgggatatc ggcggtgtta ttggaaaccc
     2461 ggcagatttc tttttgatga gacatgttac aagcttcccc aacatgtttc atggtgcaac
     2521 gattccattt ttaatgttct ttctttacca actgatgttt atcgtgatta ccgttccttt
     2581 gatgacagga gcttttgcag ggagattgaa tttaaaaggc tatattatac ttgttatttt
     2641 ttggaatctg ctgatttatt tccctgtttg ccactggata tggggtggcg gatttttgga
     2701 tcagatggga tttagagatt tcgctggtgg agcagtcatc cacacgacag ctggattcgg
     2761 ctctttagca tgtatcctca cacttggtca aagaaagatt cctttaggag aaagaagtca
     2821 gcatagcagt cttatggcag ctgctatcgg tacaggactg ctttggtttg gctggttcgg
     2881 ttttaattcc ggtggtgcac ttcgagcaga tgaacaagca gtcaatgcat ttggttcaac
     2941 attcatcgct cttgcatttg cgatgattac ttggctgatc attgcaaaag taaaaggaaa
     3001 tggctttgat tttgtcgacg tactaacagg atccgttgct ggattggcag ccattacgcc
     3061 ttgtgccggt tatgtagaag caaaaagtgc catgctgatt gggatcattg cgggtatcgt
     3121 ctgtcatgct gctgttgatt ttcgtaaaaa gaaacaatgg gatgatgcac ttgatgtgtg
     3181 gggtgttcat ggcatgggcg gattcacagg tacgattctg atagggatct ttgcatctaa
     3241 cagccattta ctaacgaatc caagcagctg gtacttctta gggattcaag tgattggtgt
     3301 catcattact gccgtgtatg cctatgtgct gacaacagtg attttaaaat cggcgatgca
     3361 ctttacaacg atcactacta caaaagaaga acaagaagaa ggattggact ttttcttctt
     3421 tggcaacaaa ctcaattgaa acaaattcaa atagaaaaga ggaagattca tgcaacaaag
     3481 caaaacgaaa attcctgcaa aaaaattaac gttctttggc tttttggcta tgaccatctc
     3541 aatggtcgtt tcattatatg aatatcccac attcgcgacc tcaggcttca gcttagtatt
     3601 tttcttgtta ttaggcggtt tgctatggtt tatccctgtg gcattgtgcg cagcagagat
     3661 ggcaacggtg aaaggatggg aaaaaggcgg tgtctatact tgggtcagtc gcacattggg
     3721 aaaacgattc ggttttgccg caatcttttt ccaatggttt gagataacag ttggctactt
     3781 gaccatgctg tatttcttaa ctggtgcatt atcctatgca acgggtattg gtgctataca
     3841 aaacaataaa tatctgaaat tggcaatcct attgattatt ttctgggcta ttttactctc
     3901 acagttaaaa ggaaccaaat acacttcatt gattgctcgg atcggttttg tagctggtat
     3961 cttgttgcca gcattagtct tgtttggctt agggatccat tatgtcgctt caggcgctcc
     4021 actacaaacg atactgtcta tgaaaacatt gattcctgat ttttcgaaat tgcctacgct
     4081 ggttgttttt gtttccttta ttttagcata tatgggcgta gaaacatctg ccagtcatgc
     4141 aaatgaaatg gaaaacccta aaaaggatta tccattagca atgtttgtat tggttattct
     4201 ggcaatcatt ttagatactt ttggcggatt gaccgtcgct actacagtac ctcaacatgg
     4261 tttgtctctt aatacagggg tgattcaatc gttagaattc ctattaagac acattgatcc
     4321 aaatcttgca tggattgcga aaattcttgc gattcttgtt tgtttaggtg tcatcggaga
     4381 aattgcttct tgggtaacct ctccttcaaa agcacttcac gtagcagcag aagaaggatt
     4441 acttcctgaa tattttgcaa aagaaaacac acacggtgtt cctgctcatt tgatgatcgc
     4501 aaatgggatc gttgcaacaa tctgggcagc cgtctttaca ttaagcggcg gggggaataa
     4561 tctttcattt cttgcagcga tgtcattgac tgttgtaatc tacctgatgt tgtactttat
     4621 gtttttcgcg ggctatttga agctggtttt taaagatgac agtcttgaaa gagcctatca
     4681 ggttccaggc ggaaaagttg taaaagtaat cgttgcttgt ttaggtcttt tgacttcact
     4741 ttttgctttt tgtatctcat ttgttccacc tgctagtatc gcagctggac agcatgggat
     4801 ttatgaaacg attctgattg tttcttatct tatcgtctta gttattccat ttacgatcta
     4861 cgcatgccgt cataaattcc agcatgcaga aaaataaaaa atgaaagctg gttattcaaa
     4921 tggaagtaaa agaattagaa caattgattg gaaaaaattt ccactattat caaacaggac
     4981 aagtggcgga ttacattcca gcattagctg aagtggatcc caaacaatta ggtatggcga
     5041 tttatgattt ggatacaaaa caactgattg agggaggaga cgccgatgtg cgctttgcca
     5101 tcgaaagcat gtcaaaagtt cccgtattgt tattagcaat caaagacaac ggaatagaaa
     5161 aagtctttca aacaatcaac acggaaccaa ccggttttgc cttcaattct atcatgaata
     5221 tggaaatcaa tcatcgcaaa catccaatga atccttttgt taatgcagga gcgattgcaa
     5281 caacctcgtt gattgccggt aaagatgcag atgaaaaatt tgagcgaatc ttgtcattta
     5341 tgaaagagat ctgtgatgat cctaatatca cattgaatga agaaatctat cattctgaat
     5401 caagaacagg tgatatcaat cgctctcttg cttactatat gaaaggtaat caaatgatcg
     5461 aaggagacgt accggagata ctcgatgctt atttcagaca atgctccctc aatgtcaccg
     5521 ctacaggcat agcaaaatta gctgctgtgc tggcaaataa aggaattgct ccttggaatg
     5581 gcaaacaaat cattactgaa gaaagtgcta caatcgtcaa atcaattatg acaactgctg
     5641 gtttgtatga tgaatcagga gagttttccg tccatgttgg tgtacctgca aaaagcggtg
     5701 taggcggcgg gttgatggca gctgtgccaa atcgttacgg cataggtgtc ttcagcccgg
     5761 cactagatcc atttggtaac agcgcagctg gtatccaatt gttgaaagat gtagtcaaag
     5821 aactagacgc tgacatcttt gaataaccat tcttgaaaga ggctggggta aaagtccaaa
     5881 cggctcaaag ctacctacaa tgaggctgaa attgtgagct tgtttgactt ttgtcccagt
     5941 ttcttttttt attggttttt gaaagggctt ttctaaataa aaaggaaaca aaaatacgat
     6001 ttacagatat gattaaataa aatcataaga aaaactggag gcgtgtatca ggaatgctgg
     6061 aaaattacat cgagcgaaat atatttagaa aagtgtatct gtgtgaacaa ttatttgaat
     6121 tccaagaaat agacatcgaa caaacagcta tttctctaag ggtgactaca ccaacgatcc
     6181 ttcacgatct agaaagtcta gccgagtgtc tagaatattg tataaaagaa caagtaagag
     6241 agaagcataa gtataaactc gtattcaaac atggtattgc tctttctgag ctcacacagt
     6301 ttctgtacgg tcaatcttat tttttaaagt ttttaagtta ttattttaat ggtatattca
     6361 cgagtacaga actggctgat cttgaattta tttctttgag caaagtatat acaatcaaaa
     6421 aaatcgtatt ggattttttt aaagaaaatg attatttgaa aaataaagaa atcattatcc
     6481 cagaatttga ctcgcggaac atcttgctag ctttagtacg ttatgtcaat tgggaaggct
     6541 atgaaaataa aaatccgtat gtagaagaag cttgtaacga gttgattgat tatgtagaga
     6601 ctcatttttt taaaagaaga tattcggaag atgaaaagtc ctttatcata cgaggaatag
     6661 agatcgcaat cggaagaaag gcatttcccc ttggactgaa agaagaagat aagaaaagcg
     6721 cagaaacgaa gccgttattt caattagtct gtcaaggatt ggaagagcaa aaaggaattc
     6781 tagacttaca agaagaagat gtctattata ttttttcttt gttcaacagt agaaattata
     6841 ccaatgagaa catggagctg ttaagaaaag atttcgaagt tgtttataaa aattttgttc
     6901 tcaatgacct atcactgaat gaattgattt tacagattat tagtatcgct gaaatcgacg
     6961 atgcagaaaa ttttattttc aagcaatcct ttttacaatt tgtccgtacg ttgtgggcag
     7021 atggacaagt atttttaccg gaaaggatct atcttctatc agaaaaagaa caacttctgt
     7081 acggaaaaat tgtagaaata ttggaagagt ggaaaaagaa aaatcaaatc caactaagat
     7141 ggaatgaaaa tttcattcga aaattcgtca aaagtctttc tttgatcaac agtgaacaag
     7201 cggggagaca ggaaatagaa atttttgttg tttcagaaag ttcagtcaaa caattttttt
     7261 atcgaaccca atttcatttg tatgcggaag aaggcatcgc tgaaataaat cctattttat
     7321 ttcgttcatt agaagaattg cctgatgaat gtctatgtgc gaaaaaaaga gtggttttgt
     7381 gcgatgctgc tagctatcaa aatggattag aaactgaaaa gacaaagatc tatccaatct
     7441 cattaaaagg tgcaaacatc catttgtatc atattgctca ggaacttcac ctattaaaaa
     7501 aacatatttc ttgatgagac cttaattagg agaccttcta caaagctttt taattgtctg
     7561 tagaaggttt ttatttgagt cctgattcgt gcgaagatct acttatataa caagctttct
     7621 ctgaaatttt tttaaaatgc tattgactta gagtcaactc taagtagtag aatagataaa
     7681 gtgtaacaaa tctattttca ttaaaagaag atagtacgga ggagagaatg tattatgagt
     7741 acagtattac atgtattgga tgcaatcaat agccccgcag atgtcaaaaa attatctgtt
     7801 gaggaaatga aacagttggc agaagagatg cgccaaaaaa tcttgcaaaa ggacagcgat
     7861 gtaggtgggc acgttggtcc gaatctaggt gtagtagaat taacgattgc gtttcattat
     7921 gtatttgatt caccaaagga taaaatcgtt tgggatgttt cccatcagtc ttatggtcat
     7981 aagcttttaa ctggtcgaaa aaatggtttt gaaaaaggac attaccgtga agtgagcgga
     8041 tattccaacc aacatgaaag taaacatgac tattttacag tggggcacac atctacatct
     8101 atcagcttag caacaggttt agctaaagcg agagatctga agaaagaatc aggaaatgtg
     8161 gtagcattta tcggtgacgg ctccctaagt ggtggcttag cttttgaagg attgaacaat
     8221 ggtggtgttt tacaatccaa tctaatcgta ttagtaaacg ataatgagat gtctattgat
     8281 gagaatcatg gcagtttata taaaaactta gctgagcttc gtcaaaccaa cggtaaaagc
     8341 agcaataacc tatttaaagc aatgggattt gattatcgtt acgtggataa tggaaacgat
     8401 ctggaaacgg tgatccgttt atttgaagaa gtaaaagata ttgatcatcc gattgtactt
     8461 cacgtccata cggaaaaagg tcgcggttat cagccggcaa tcgataataa aatgaaatat
     8521 cattggcatg taccatttga tctgtccacc ggggaggcaa agcaagtaac ttctggagaa
     8581 acatttaata tgatcatcat ggattatttg gataagaaaa tgcaaacaag tacagctcct
     8641 attgccgcag tcaatgcagc gataccaggc gcctttggac tgaaaacatt ccaagaaaaa
     8701 catccagatc attattttga cgtggggatc gctgaacagc actctatcac tttcgcttct
     8761 gctttggctt ctcagggagt acgtccagtc gtcttccaat caagtacttt cttgcaacgg
     8821 gcatatgatc aattatcaca tgatttagcg atcaacgaga atccagtgat tattttagtc
     8881 ggtggcggaa cgatctcagc tagtgaccga acacaccaag gaacatttga cattccttac
     8941 ttatcaagta tcccgaacat caaatatctt gctccagcaa caaaagagga tatgatctct
     9001 atgctagatt gggcgttcga acaaacagcg cagcctgtag caatccgaat tcctacacat
     9061 ggtgtagaac atggtacagc tatcgcaaat gattatagta ttccagccta tcaaacagta
     9121 aataatggag gaaaagtagc aattttagct ttaggcggtt ttcttgaatt aggaaaagaa
     9181 acagtaaaag cttttagaaa aaaaggaatc gaagcaacgt tgatcaatcc aggaacgatc
     9241 acagaaaatg acgtagaaac attagataaa ttaaaagaaa cacacgaatt agtcatcaca
     9301 ttagaagatg gtagtttgtc aggaggattt ggcgaaaata tcagtcgtta ttatggtgat
     9361 tcttcaatga acgtattaaa ttttggtgca gagaaagaat tcacggatag cgttcctgta
     9421 gggcagcttt atgaacgtta tcatttaaca ccaaaacaaa tcgttgaaga tagtctgcgt
     9481 gtcttgaata ttcaataatt cattatgctc accatccaga taattgttta tactagtatc
     9541 tggatggttt tttcattata aaaagcaaag gggtggatgt tatgtatcag caagtagaag
     9601 aatatgtcaa aaagttccaa gaaaaatatc ctagttatca atcagaaaaa ttaggtttct
     9661 tttcatttgg ggatacagaa aaaatggcag acgagttgag cgaactagtt gtaaaaaaga
     9721 taaagactgg tacatcaagt ggttacgatc tgtataaaca agagaatgag cctttaccaa
     9781 aagttggtca attagacgtg gtcctgaatg gaaaaaatga accagtttgt atcatcaaaa
     9841 atataaatgt gaccatcttg ccttttagtg aagtcacgaa agagcatgct tggaaagaag
     9901 gagaagggga tcggacatta gcttattggc ggaaagtcca tgctgagttt tttaaacatg
     9961 cttataaaga agcgttgaac atagaattta cagaagagaa gctggtcgta tacgaagaat
    10021 ttgaagtaat tttcttttag taagaaaggt ctgctgaaag aaatagctca ttattgtctc
    10081 ctgaatagaa aagggttaca tttttatatg aagctgtatt tttatataac attactggag
    10141 gagatattgt ggatacactg gaacaaatca aaggcggagt cgtcgtttct tgtcaagctt
    10201 tggaaaacga gccgctacac agttctttta ttatggggag aatggctttg gctgcaaaag
    10261 aaggcggagc agttggtatt cgagcaaata cgacagaaga tatcaatgaa atcaagcgaa
    10321 tcgttgattt accggtaata gggattatca aaagagatta cgatgggtca gaagtgttta
    10381 ttactgcgac agaaaaagaa gtggaagaac ttttggagac gcaagcagaa atcattgcac
    10441 tggatgcaac ttctcgtaaa cgtccgacag aaaaaacaac tgctgaactt gtccaaatga
    10501 ttcacgaaca cggtcggtta gctatggcag atatttcgac ttttgaggaa gcgcttcaag
    10561 cgcaagaaga tggttttgat cttttgtcta cgactttagc aggctataca gaatattcac
    10621 gaaagacgca gacacctgat tttgatttat tgaatcaaat catagaacaa gtcgatatcc
    10681 cagtaattat ggaaggacat actgactctc ctgaacaagt agagaaagca tatgaattag
    10741 gtgctttttc agtcgtagtt ggatctatta tcacccgtcc gcaattgatc actaaaagat
    10801 atgtcgaagc cgcaagtaaa gcaaataact aaaaaacacg cacggcacag attcttgaca
    10861 aaaaagaaaa attgatagat agtttaaagc agattctaag aagatgaaaa aagcattact
    10921 tttcattttg tcttcaagat ctgcttttta acgaatttta tagaaagaag gcgaaaccat
    10981 gtttgaacgg agtccttcag aattgacaga acgtgaaaat tacaagttgc tgattggttc
    11041 agtcatccct agaccagtag cagtagtaac gactaaatca gcaaaagata tagtgaatat
    11101 cgcacccttc agttacttca atatcgtcag ttcgaatcga cccatcttat ccttggcgat
    11161 ccagagaaaa caagatgcag taaaagacac agcaaagaat attcttgaga caagagaagc
    11221 tgttatccat atccttgatg aggacaacgt agaagaagta aataaaacag cagccaatct
    11281 gcctaatgag gaaagcgaac tgtacctgac tgacttgact ttaactgatt cagtaatcat
    11341 ttcagtacca gcattgacag aagcaaaagt tcgttttgaa acatccttat atgaacatgt
    11401 cgagatacgg aataaaaata aagtaacagc tgatttattt ttgctagaag tacagaaata
    11461 tcatttaaat gaaacagtct atgatgataa gacaggaagg atcgatccaa gaaaattgaa
    11521 tgctgtaagc cgtttggcag gaaatgatta tgcagcaata ggagaaattt tcacgattgc
    11581 acgtcccgaa taaaaaggag cactagttat gttaaaaata agctataaac catcgacaga
    11641 ttcaaaagaa atgaaaaaag aatatgaaac agtgaatgat tttctgcaag ggcagtactt
    11701 agaagttccg ccattgcaag accattttgt cgttactact gttactttag atggtaagga
    11761 aatagaaatg ccagatcaaa cgattagcgg attgtttaat tactttaaca aataaatatt
    11821 tattccgaaa agtgaaaaaa catataaaaa aacacataaa actaaaaaat tatcaaagaa
    11881 tcctggtgca actgaaagaa tttgcaacgg gattctttca acttatttgt tatttttttc
    11941 taaaaaatat taaagattgt taaatttcac tatttcattt caaattccat tgcctaatca
    12001 ttaggaaaag tctatcatga actcgtaaat agaatatagt aataaccttg ttaggtgagg
    12061 ctcctataca aacataggct gttgcccaga aacgtcgaaa gtcgccaatg ggtaaaacag
    12121 aaactgttgg attaaggctt tttccaatag ctaagaatgt tttctttacg ttgtatagtg
    12181 ctaaaactcg gacgatgaga tgatgctagg tcagaattct tgttcgaaaa ggtttctgac
    12241 cttttcttta tatttttgtg actgacttat ttgatgagac aaactcgctc ctcacctgac
    12301 aagtggattg ctgtgtgagg aggagatgcg gtgaaaaaag atcgattggt ggaggtcgct
    12361 cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta
    12421 tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt
    12481 actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag
    12541 ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc
    12601 ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca
    12661 aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca
    12721 aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc
    12781 agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta
    12841 gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag
    12901 acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg
    12961 aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg
    13021 gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag
    13081 aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca
    13141 agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat
    13201 tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct gatgtcaaag
    13261 gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac agtttttcat
    13321 ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat aaaggaacat
    13381 tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt tttccagaaa
    13441 tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg aatattatag
    13501 atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga gtcgttccaa
    13561 gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct tcctccttcg
    13621 acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa cccgattact
    13681 ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga gctaccattc
    13741 aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg attggttacg
    13801 tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt aatggaaaaa
    13861 tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca acaggtggga
    13921 aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa catgaagttc
    13981 aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaata gcttttgaca
    14041 gtctaggagg aaaagctgca tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg
    14101 cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc tcacaagaag
    14161 attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt gcgacaggtt
    14221 atgctcctgg atctacgttt aaaatgatta cagcagcaat cggtctcgac aacggcacta
    14281 tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac agttcttggg
    14341 gatcttttaa aacaacgcgt gtcagtagtg atgtatcaca agtagactta aaaactgctt
    14401 tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat
    14461 ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca attagtatga
    14521 atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta gctgatactg
    14581 gatatggaca gggcgaactt ctaattactc ctatccagca agcagcaatg tattctgttt
    14641 ttaccaacaa tggcacactt gtctatccta aattgattgc agataaagag acaaaagata
    14701 agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg agagaagttg
    14761 tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg ttggcagcga
    14821 aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag aacagtttct
    14881 tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg gaaaataaag
    14941 aagatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc aaccaaaatt
    15001 atcaataaaa aattgctttt taagtacagt aaaaggactg tggcgttatc gtcacagtcc
    15061 ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg atttactcac
    15121 acttcataaa ctttttttgt aaaatatttg agaacaagtg gagaaataag tgtacttaat
    15181 aaaatcacga taactaaagg tgaatagtac tgtggttcga tcaattgact ttgctgtcca
    15241 atttgtaaaa tgatcaaagc catttctcca cgcgaaatca taccagcacc gaccatcaat
    15301 gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat aacctccaag caatttggtc
    15361 aaaatcgcta caagcgtcaa tatagaaata aagagtagtt gttcactaaa gtcagaaaaa
    15421 tcaacttcta atcctacact gacaaaaaat actggtataa atactgcata tcctaaagcc
    15481 tctacattgt ggtatacttc ttctttaacc tttgtctgac tgacagctat cccagcaaaa
    15541 aaagcaccaa tgacagagct aagcccaaca agatccgcta cgtaagacat tcctaaacaa
    15601 ataactagcg atacgatgat aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt
    15661 aggaaaggcg caatccattt gactaataag aaaataaaaa caaaataaat tagctgttca
    15721 atcaaaacaa gcggtaaaga agcattgcta gaagcttccc ccgttagaaa agataggctg
    15781 aaacttagaa ctaaaacgac taaaatgtca tctaccacag aagcacccaa aattgttgat
    15841 ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat actgacagaa
    15901 gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg
    15961 cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac gaacatacca
    16021 ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa aaacattaat
    16081 aagatcacac caatctccga aaagtcatgg actagaatat caggtttgac ccaattcaat
    16141 ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg gatacctaat
    16201 ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa aataccaata
    16261 aattccattc tcatcattcc tttgtataaa atatagcccg aacttagcat aacatacatt
    16321 attgaaaaaa taggaaaata tactcacaaa aaaaccaaca atccaaaaga tttttttgga
    16381 ttgttggttt ttatacggtc tattttaata actaaaaatg attaacgtat agtcattttt
    16441 cttattctga tgaaattgat aatctcctgg atgcccatga aaatcaaaat aaagccgaat
    16501 atttggaata gaagcatcaa acttttgaat ggattgaaca ataagacgaa tccgccaatc
    16561 aacaacagaa tactactata aacactccaa cttttagctt ttgtattcat gctgaagaac
    16621 aactggaaca agctgttgat gataacagct aatccaagaa gaatcggcag gatagaaacg
    16681 attgcaggtg caaaagcata gacgatcaga gctaagatca ggaaaaaaag accactgaac
    16741 agaccgatac tccaagaacc tgttttcctt ttaaaacggt aagcctcaaa aagattcaac
    16801 aacccaagca aaacaagata ggcggtgatc agataaccga taaaatgaaa aacagcagaa
    16861 gggtttagca caatcgcaat cccagaaata atataaacag cagctcttaa taaggcgtat
    16921 ttttgaatat tttgtttaat cgtctcaaac atataagaaa acctcctttg acttatttta
    16981 acgtgaacta gtcattttac caaattaaat gttacaaaat gagcgaagga gaagaatatc
    17041 tctggaaaaa tcaacttctg aggatctttt ccattttttt gccttttgct agctcatcga
    17101 tcattttatc taaataacga atttctttca tcaagttatc ctcgatgtct tctactcgta
    17161 caccacaaac aacacctgta atcaaggaac gtgaaggatt ggggacaggg gcttccgcaa
    17221 aaaattgttc gaaagtcact tgtttttgta gttgatattc tagttctgtt tgggaataac
    17281 ccgtcagcca acaaatgatt tcatctactt cctcttttat acgatctttt ctctccgctt
    17341 ttgctacata cataggataa acgcttgcaa aacttgtcgt aaaaatacga ttttgcgcca
    17401 taatcagtca cctttctttt tttcaaatta tttactactt atagtatata agaaaaagag
    17461 aagaaaaagc gattattcaa aaaaacaaaa aggattgtga caaaattctt gtcaccatcc
    17521 ttttttccgc tctctgactt tttacaatct tcatagctca agtgcgcatc agataatcga
    17581 atgctcctaa agctgcagta gctcctgaac ccattgaaac aatgatttgt ttgtatggac
    17641 tatctgtaca atcaccagct gcgaaaacgc cggggatatt cgttgcgcca tttttctcgg
    17701 tcactatttc atttcttgga ttcatttcga tcgttctatc tagccaatcc gtattaggga
    17761 gtaagccgac tagaatgaat actcctgaaa cagctagtgt atgcatctcc atcgtacggc
    17821 gatcgatata ggagagagat tctacctgat tcgtaccgct gatttctttc gtcgctgcat
    17881 gtttgataac agtgacattc gcaagagaga ctagtttatc ctgtaagatt tgatctgctt
    17941 ttaattctgg taagaattct agaacatata catgttttgc taacccagcc aaatcgattg
    18001 ctgcttcaac accagaatta ccaccaccga taacagcaat ctcttttcct ttaaataaag
    18061 ggccgtcaca gtgtgggcaa taagcaattc ctttattttt aaactctttt tcgcctggta
    18121 cattgataga acgccagtgc gcacctgtcg cgatgatggc ggtttttgct ttaagtgata
    18181 taccgttagc taagccaata ttaataaaat gttgctcttt atttaaggaa acggcctgtt
    18241 gattcatcat aatgtcgata gggtaactac gcacatgact ttctacttgc tgcatcaatt
    18301 ttggaccttc tgtgtaagag gtaccaatca tattttcaat ccctagtgtt tcaacgactt
    18361 gtccgccgaa agaatcagcg acaagtcctg tacggattcc tttacgtgct gcatagatag
    18421 ctgcactact agctgctggt ccaccgccaa tcactaatac atcataaggt tctttttcaa
    18481 acaactcttc ttcaatcaaa gggccagtga ttttttcaac aatttgttcg atcgtcatgc
    18541 gtccgctact aaattcttct ccatttaaaa acacagttgg cactgccatg atttttttat
    18601 catcgatttc tttttggaac atcccacctt caatcatcgt atgagtaata tttggattta
    18661 atacagctaa tatattcaat gcctgtacaa cgtcggggca attatgacaa gtcaagctaa
    18721 cataactgtc aaaatgcaat gtttgtgtta tttttttgat tttaagactc aaagattcac
    18781 tgattttcgg tgcccgtcca ctaatttgta acatagccag taaaaaagag gaaaattcat
    18841 gaccgagggg gataccagca aaaacgattc cactttcttt gccaactgga ttgatagaaa
    18901 aactaggtgt gcgcatcgaa tctctttttt cccaagagat tttaggagac atttctgcta
    18961 tttccttcgt aaattcagct agtttttgac tattttcact attatccaaa ctaacagaaa
    19021 aaacaatagg agattcaatc agtgctaaat attgttcgag attttgttta gtttctttgt
    19081 ctaacatatc caccacctaa atctttccaa caagatcaaa acttggtttc aatgtttcgc
    19141 cactttcttt ccattttgcc ggacaaactt cacctgggtg ggtacgtaca tattgagcag
    19201 cgcgaatctt atcaatcagt gcactagcgt cacgaccgat tccatctgca ttgatttcca
    19261 tagcttgaac gattccgtct ggatcaataa taaatgttcc tcgctgagct aatccttgct
    19321 cttcatccaa aacatcaaaa atacgagaaa tctgatgaga cggatcaccg atcatcacgt
    19381 attcgatttt accgatagca tctgatgtat catgccatgc tttgtgagta aagtgggtat
    19441 cggtagagca agaaaagact tcaacaccta aagatttcaa tgtactgtat tgttcttgta
    19501 agtcttctaa ttcagtagga caaacaaaag taaaatcagc tggatagaag caaagaatac
    19561 tccagttccc cataagatct ttttcactga cttctaaaaa ttctccttga tgataagcat
    19621 gtgcattaaa ttcttcgatt tttttcccaa tcaatgacat agtaaaacct ccaataataa
    19681 ttatttattt ataattattg taatttagtt ggagttgcaa agtcaatgga tattttgtta
    19741 tttaataaag attactcaaa aacgaaggtt aattataaaa atagaagagg tcgcaagaaa
    19801 atgctactgc tggaaatact cgggataacg ttgagtaaca gtttcctgtt ctttcagcat
    19861 catgtgtaag tgcaatcccg tttgaaatcg agcttcttct ggttttttat caactaaagc
    19921 cttgagtaaa tcataatgtt gttccatgat cgtttcccat ggaagcccct gaatggtcac
    19981 tcgtaaccag cggaaacgtt ctaaatgggt attatggtca tctaaccagt tccaaacttc
    20041 ttttcttcct gcaatttcga aacaagcttc atgaaataaa ttatctgcac ggaaaaaagc
    20101 gcgttcgtca tttattttga ctgctttttc ttgttcttct aaaatcgttt ctagcactat
    20161 tcgtgtttgt gtgtccaatt tggaacaaca ttcaatcatg atttgctctt ccaactgttc
    20221 tcggacaaag cgagcatttt ctgcagaaac aagattaatt ttggaaacat acgtaccact
    20281 ttgaggtatc gtatacacga gttcttgctg acgtagttga atcaatgctt cgcgaatggg
    20341 agtccggccg atagcaagat gttcctccag tgccttttca gatattttcc ttccaggttc
    20401 taagtcagca tagataattt gtttacgaat cgcttgataa gcttgtgatt gcaagctgct
    20461 tttcatcttt attcctccaa aatcatttct atgaacgtag tataacagaa gaggtctgaa
    20521 aaacaatctg gtatgtcaca ttaactagta aatttgtata aatagtaaaa ttttggttga
    20581 catagcgatt tttattgcat aaaattcact tgaatacaaa aactaatatg ttagtttagg
    20641 aggggaaaaa tgattgattt aaacaactgg aggaacttta tccctgaatt atctcaaaaa
    20701 gaagtagaaa tacctcattt tgaccgtgca tcattaagag aaaatggaca aaaaaatcct
    20761 gtctggatcc atttcggtgg cggaaatctt tatcgaggat ttcatgcaga aatcgctcaa
    20821 cgtttagcgg atctaggcga attgtccact ggtgtcattg tttgtgaaac attcgatgaa
    20881 gaaataattg aaagggctta ccaagatttt gatgatttga ttcttgaagt ggtcatgcca
    20941 agcagtggag agttcgtcaa acgtatatta gctgcgacag ctgacagtct gtattgtcat
    21001 ccagattgtt ctgaacactt tgataagatg attcgacact ttgaaaatcc tagtttacaa
    21061 ttagttactt ttgctattac tgaaaaaggt tatcgattga aagatacaaa taataaatat
    21121 ataggaccga ttgcagtcga tataaaaaat ggtccaaaga agccatcaca tacaatgagt
    21181 attcttgctg tgctacttta taaacgtttt ttatctggag cttggccgat tgcacttgtc
    21241 tctacagata atttttcaca aaacggtagt caattacgta cttctttgct aacgatcgca
    21301 aaagaatggc aaaaggaagg ttatgtacaa aatacattta tccaatactt atcgaatgaa
    21361 gaaattgttg cgtttccatg gacgatgatt gatcgtatca ccccgaatcc ttccttgtca
    21421 gttagcaaaa tgttggcaaa agagggcttt tcaaatatgt cgattttcca aaccgcaaaa
    21481 ggaaataata ttgctccatt tgtcaataca gaggacatcc attatttaat catggaagat
    21541 cattttccca atggacgtcc gaaattcgaa aaagctgggg tattcttgac tgatcgtgca
    21601 acggtagata aagcagatac gatgaaggtg acgacttgcc ttaatccttt gcatacgtct
    21661 ttagctatta tgggttgttt actggggtat aggagcatag ctgaggagat gaaagatcca
    21721 gatttatcgc tgctagtttc tgaaatcggg tacaatgaag gcctcccagt tgtggagtct
    21781 ccaggtatca ttgatccaaa gatctttctg gaagaagtaa ttactgtgcg cttttctaat
    21841 ccaaatattc ctaatatgcc tcagcgaatc gccacggata cctctcaaaa ggtggcgatt
    21901 cgttatggtg agacgattaa aaaatatatg gaaatagaag ttaaagaccc gcagacactt
    21961 gtattcatac cattgactct cgctgcttgg cttcgatacc ttattggcat tgatgatcaa
    22021 ggaaaaccat tcatcccgag tccagaccca ctattgacag accttcagca taagctttcc
    22081 gatattcaac tgggtacgca agatacatca catattcatg agaaaatggc tcctattttg
    22141 tcaaataaag aaatttttgg tgtagatctt taccaagcag agcttggcag aaaaatagaa
    22201 gaaatgttca tcaagatggt agcaggacca aatgccgtac gaaaaacatt acaggattac
    22261 ttacaagaac atggaggaaa tttatcatgg aaatgacttt tagatggtac ggatcacagg
    22321 aagaaaagat taccttagat gaaatcagac agattcctgc ggtcaaaggg attgtaggca
    22381 cattgatgga tataccagta ggagaggtat ggcctaggga gaggatcaaa gaactaaaag
    22441 aagaaattga gtctcgtggg ttaacactaa aagtaattga aagcgtcaat gtgcatgatg
    22501 ccattaaaat tggattacct tcccgtgagc agtatatcga aaactataag cagacaatcc
    22561 gaaacttggc agaatatgga atagaagtca tctgctataa ttttatgcct gtatttgact
    22621 gggtgaaatc tgaccttgac taccgcttag aagacggctc atccacgtta gcttttatat
    22681 ctgatgatat tccagatgat ccaagagaaa tcgtcgcgcg catcgaacat tccagcaatg
    22741 aatttggatt gccgggatgg gaacctgagc gcttagctcc tatcaaatcc ttgcttgaag
    22801 cctatgcatc agtagacgaa gaaaaacttc gagaaaattt tgcctatttt ctccaatcaa
    22861 tcatcccaac atgtgaagag gtaggggtca aaatggctgt acatccagat gatccgccat
    22921 actcgatttt tggattagct cgaatcgtaa agaatcgaga agatttagat tggttgtgtc
    22981 gcatagtaga cagcccttat aacggaatca ctctttgtac agggagtatt gcagaagatc
    23041 cagaaaatga tgtatatggg attttagcag aattttgcca aagagatcga atcccatttg
    23101 cacatgttcg aaatattaaa tttttagcta atcgagattt ctatgaggca ccacataaat
    23161 ccgaatacgg ttcattagac atgtaccgca tactaaaaac aatgtatgac aacggattta
    23221 acggatacat ccgtcctgat catggaagaa tgatctggaa tgagacagga agaccaggat
    23281 acggcttata tgatcgagca ttaggtgctg tttatctaaa tggattatgg gaagcaatcg
    23341 aaaaaggaaa caaaagtaaa gataattagt gaaaaaaact gtaattttat tatgtaaata
    23401 agaaggatgt tcaaattctt gatacataaa tccggaggtt gttaataagc attcggattt
    23461 atgtatctta tttggtctag gaagtaactt gattttcttt tttcgattga tccatgagtg
    23521 cttcttcttt taattcaaag atatattctg agatcctcat aatcactttt ttccagtctt
    23581 ctggtgtgat gattcctttt aaaatataga tttttttgat atgagaaggc aattgtttca
    23641 aagggcagtc gctgataacg atgttgcagt tctccataaa atcattttgt attttgatcg
    23701 attcatttga aaaaatttgt tctatcttaa cgatcagatc tttactgata taaaaatcac
    23761 gcgtatagtt cacaccaatc acaattcgcg gctgtttttt gtagcgatca tacaaatgta
    23821 ctaggtcttc aaccatccat tgcacatttt ccttttctaa aataactggt aacaagtgtt
    23881 gattttgttc tctgaaatcc atataaaatt gttcaatggc ggtttggact tctttcaata
    23941 cgctatgttc ttgataaagc atgaaagtag gaaggggcgt atttggttga ttgaagttaa
    24001 gaaatccatt ataaagttta tttcgtgcga atgccaatga gtaaagaatc ttttcttttt
    24061 caggtataaa caaatcaaat acagtacaaa gggactccac catttctctt gaataaagaa
    24121 catgaggaac gtttgctttt tttaaaagtt tgtattgctc caagcttgtt tcttccgatt
    24181 cgattttcgt gatagaaagt cgagctaaaa tattcactaa gatgcgttgt tcgcgacaaa
    24241 ttggcagatt aggtcgcaaa atatctacag agagatcaat aaaaaagtca atatattcat
    24301 actctgtgaa ttctttttgt acgtctttca cagaagttgc aggaaaattc tctttacata
    24361 tttttaatag aaaatataat ttatctatca taccgctatt tagtcggtca agtacttctt
    24421 ttttaaaaaa agtattcatc ataaaagcca ctacttgcgg atcttcatca taaaattctg
    24481 tagcagtact tgaaaagatg ttccaataaa tatctaatgc tgcatattga atttgtacct
    24541 cttttccaga aaaagacttt ctgccattat tagaaaagga gatagacaaa ccgtactatg
    24601 ctaaaaatgc attaatcttt ttcattttcg tatacaaata agaggcacta tagtttgttt
    24661 gctgactgaa actgataatc gaatttgtct gacagctgaa caaagaagtc aagatctgat
    24721 aactgactga ttcgttgcag tatcttccaa atagacgata atagcagtaa gccggatcaa
    24781 ttccttcaaa attcaacttt aaaaaaggtt tttgcttatc aatatgcagt tctgttagat
    24841 tgagacgctc aaagtcttgt tctaattctt tgatgtatag taaaaaattt gattcagaca
    24901 tatggaaatg atctgctaaa tattgtgtag taataaagaa acgatgttca gataggaaaa
    24961 gaaaaatatc taattttgta ttgctttttt tcttcaagat aaacgtttta atcgcttctt
    25021 gagtttgcat gaaagaggct cctttttact taaattgtta aaacttgaaa atattactgt
    25081 ttttttcata aatttagaca aaaaaacagt aaacatatag gtagaatctc tactatctta
    25141 tttcttgcat acaaaaaaat caaggagtga gagagttatg agatattcaa ccatcttaat
    25201 atcaacattt gcagccttaa gcttatcgct tactataaat cagacagtcc acacagagga
    25261 gacagaaaca gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa ttacctttca
    25321 ggatgaattt tcatccttca attcaggagt cgcaaaatta agaaaaggac aaacagcgac
    25381 catcaaagca aaaattgact attcgggaga cttagcacct atactacaat cagctgttac
    25441 atttgagaat gttgatccca ctttgtcgat tggctatgac gagaacactt tgactatcca
    25501 acacaagcaa gcgatattta ccttcaccgt tacattaaat caggacttgt taaaaccagc
    25561 gctttttact gtcaaggtat cagatggact accgaacgat catcatttaa cgccgtatag
    25621 tcagcggcag acaattgagc aagattcagc catcgactca ggaggagatc ttgttgaaga
    25681 accaacagat aaaccagaaa atgaaaataa gccagaagta ccgcctacag aaaatccaga
    25741 cggagaacag aaaccggaaa tagaacctgg agaagaaccc gatacagaaa ctcaaccaga
    25801 gccagacaat aaatcaaaac ctgaaataac ccctggagaa aaaccagatg taactccaga
    25861 accagatacg gattctggaa accaaacagt accggaaaca aatccagaca cagataatga
    25921 aactgaaaat ccagaaaaac cagaggtaga tccagaagaa aaaccagatg tgactccaga
    25981 accagatacg gacgcaagag accaaggaat accagaaaaa ataaacaaaa aaacaattca
    26041 agaggatgga aaaaaagaga gtaaaaaatc aaatctagca attctaaaaa taaatgaaga
    26101 gcagctgaat aaaaaatcac gaatatttga tagcgcccaa tcagctgaaa ccttaaaaag
    26161 tagcaaggat acaacctttg ctgtcctgaa acgaaaaata aacagctacc aaaatccgga
    26221 gagtcacaaa acaaagttat actgtggagc ggaatcattc tattaagtat agcaacaatg
    26281 ctttctgcaa aaagatttaa acaaaacaga tctctctaac atttgaataa tgtctgtgaa
    26341 cgcatttttc aaataaaaag aaatatccag aaaatgaccc caaaatacca gattgaaatc
    26401 tgatatttgg ggtcgttcat actttatctt tcttcatatt tattttaagg gatatctgtt
    26461 tcttttggaa gatggttcgg gatctttttc actttcaata catctaaaaa gaaggtaaag
    26521 atcggaatgc cgacaatcaa gccccaaacg ccaaagaacc gttcgctaac aagtaagacc
    26581 acaaatgtat aaaaaatcgg cagttctgtt ttgctggaca tcaacttcgg attcagcaca
    26641 taagactcaa ttagatggac aatcaaaatc gtaatcagga tataaacaac atctcggatg
    26701 cctccttggg aataagctat gaaagataga ggaatacaag aaatgataac acctgctact
    26761 gggatcagac ttaaaatgaa aatcatgatc gctaaactca gcagctgatg aaagccaaat
    26821 acagccaagg cgattgtagt gagcaacgta tttaaaagag cgacaacaaa ttgagcttct
    26881 aataccaaac caaatgtatt gacaaactta tcggcaaaat agtaaatatc ttgaaagaac
    26941 caagaaaatt cactttttaa aaacaatctt gagaatacaa ttgttttttt cttttcaatc
    27001 ataaaaaaga aacttaagat gaatgacata gcaaacgcca ctgccatttt tcctatatcc
    27061 tgtaaatagc caagcgcaat cgaagcgcca ttttgaagct gttcaaaaaa atcgtttcgc
    27121 tcaatataag tatggatata ttgtaaaaca ggatctgcat cttttggctg atgctcgtaa
    27181 aaattaataa cggactggat catgtcaaat gtttgatgaa taagcatcgg gatatacttt
    27241 gtgaatgcca gatagataag gtaaacgatc agtgtataaa tcatgatagc taaaacacct
    27301 gtagggattc ggacaaatcg ctgaatgatt tgaacaagat gaatcatcaa ataagtaaag
    27361 ataaatgtga gcaaaatggt tgtaatcata cttctaacta agaaaagaac aaaaacgaca
    27421 aataataaaa cagtgagacg gcgtattttt tcattcgcca aaaaacgctg ataaacggtc
    27481 aaatcgacaa cctccaaata acaaaaaata aacacgttat tcattatatg atggaatatg
    27541 aaaataagaa agagcaaaaa gaaaaaccgc taaatgacgt tatcttatga gttggttttc
    27601 ctcataaaat cgttcatcta gcagtttcag ccctttttct ggtcttgatg aaaattatct
    27661 agcatttttc gggtcagatg ttgcagtgta tccaattctt cctgttcaag tggaagataa
    27721 gacttgatct tttgtggaat accttctgct tgtttttgca acgcagcacc ttgagcagtc
    27781 agcgtaacat ttacgattct ttcatcaccc gtttcacgct gacgattaat ataatttttt
    27841 tctgctaact tttttagtaa gggagtcagc gtaccagaat ccagacacaa atcatttcct
    27901 agctctttca ctgaagtctt tttcttttcc cataaaacta acatcacaag atactgggta
    27961 taggtaagat tcagcggctt gaggagggga gtgtagtgtc taatgatttc tttagaggcg
    28021 gcatataaag gaaaacatac ttgattaact aaatatagat cttccatttt tccacctccg
    28081 ctgttttact atagtgtatc atagcggatt tacacaaaaa agtctattca ctaactgctt
    28141 tcagagtaac ttctatattt ccagctgtag cttttgaata cggacacact ttatgcgctg
    28201 cctctaataa ttcttgcgtt tcttccagag aaagaccttc gatagatcct tcaatctcta
    28261 cacctaagac aaagctcgtt tcactttctg aataaaggga aacttgtgca ctgacagtag
    28321 aagccccaga gatatttttt tgctgtttga tcaattctaa tgcactgtta aaacatgcac
    28381 tgaaaccagc tgcaaacaat tgttcagggt tggttgcatt ttcgacgcgt ttaccaggag
    28441 ctgcaatatt caattcgaat gtacgatcgg gattatgcac ttctccagag cgtccgcctg
    28501 tgttgatcat tttcgtagaa tacatttttt tcataaagtt accaccttta attatatttt
    28561 atacaattga attttacaca atataattgt ggaaagcaaa gaaatggatt ggcataagat
    28621 aaataagaga aagcacatga aacagacgcg ctttctcgaa tagcaaacat atacaaaaaa
    28681 tatcagagac aaaaaataat ctcatcataa acattacata tatttctcaa aaagttgttt
    28741 tctttcttca aaaattactt cacgatggac ctgatgaagc atccataaat aaccaaaagg
    28801 atctctgaac attgagttag tcacaccaaa gtccatcatt tctgtcattt cttgaatctc
    28861 tacacagcca tttcgtaatg ctttattatg tgtgtcctcg atatcagaga cgatgatatt
    28921 gaaccagatc gtatttggat gttcagcagt aggagcagtc aaaccaagag acgtgttttc
    28981 atccagcata tggaatctcg taccatacaa ggtaaaaatg acttcattat tgcctctagg
    29041 gtaatctgtt gcctcaatca gttgtatttc atcaaagatt gtttgataca gttctagtgc
    29101 tttcaaacta tcatttaata caaaatcgat ttccgttcct ttatgcataa agatctcctc
    29161 tccttttcag tcgaacagtt actagattct tttattatac cagctaaaga aaacgcattc
    29221 aaaaaaatag cggatttact taccaactca ttcgattgga tgataagtaa atccgcgtcc
    29281 cattacttct gctacgctgc tgacaatgac aaaggcactt ggatcgattt cttctatcaa
    29341 tggtttcatt cgggaaaatt catgatcagt cacaacgatc aataaaacct cttttgactt
    29401 tcgattatac ccgccttttg cctctagcag ggtaaggcca cgtccaatct cagcacttaa
    29461 gcgaaggcgg atgtcttcca aataatgttc actcatgacc ataacagatt tctttcgatt
    29521 gatacctgtt tcgatataag tcatgacaat tgaagtgatt gcaatagata aaatagcata
    29581 aagaaactct tctattccaa aaataaacag attaagagaa acaacgaatg cgtctgtaaa
    29641 aaataagcca atcgaagtat tcaagtggaa atatttttta aaaatgaggg gagggatcgt
    29701 cgtaccgccg ctagaagagt tgtttttata taaaatcgca actcctaaag caaaaatcgc
    29761 acttccaaaa atgacagaca acaaccgatc tgaagtaatc attgtttctg gaactattgc
    29821 aatgattaaa ggaaaaacca agcttccgaa aagtacttta aaaaagggtt ttttacctaa
    29881 aaatagaaga gctagtacca acataacgat attcaacact aaaataacta tagctcggtc
    29941 aaagcctaaa gcagcttcca gtaatattcc tagaccgctg acacctccag cagcaatatg
    30001 atgaggccct aaaaacatat tgatagcgac tcctaatatg aagagggcaa aaataatctt
    30061 aatgtatgtc catataaatt gtctcgtttc tttcatttat tcacttcctt aattcattaa
    30121 taatactctt atcttattat aacttacttt tcaccttaag ttaactttaa ttaataaaaa
    30181 aattaggcta gtctaaatta ttatgtttca ttcttctaga atatacttct acgccaaaaa
    30241 ctgttaaaaa acttttactt tagaaaattg gagaaattga tcgcttaaga taaattcagg
    30301 acactaaata gtacaggaag tagactaaaa cttttaaaag ttttaagtct atctatttgg
    30361 caagtttaaa aaataaaaat tcggtatcaa aaaactgtta ccgataacat ttttgaaagc
    30421 gttgtctttt gcttctctat cacttatagt gatgacgtca tattaaaaca caaggaggaa
    30481 atcaatggat acagctgcaa tttatcatcg accggaaagt gaatatgcat ttctatatac
    30541 gaatacaaga tttcgtattc ggcttcgtac taggaaaaat gatatcaaaa aagtatatgt
    30601 gctatgtgga gatccatata ccataacaac tgaaaaatgg tatcaaaaac aacgaccaat
    30661 gaaaaaatgt ctaagtacca atgttcatga ttattgggaa attgaagtga cgagcgaaac
    30721 aagtcgctta cagtatgcgt tccatgttgt tggggaagat aacacggact gtttttacgg
    30781 tgatcaagga atttttcctt atcgagaaaa tgtgattact gaaccaaatt tttattttcg
    30841 aataccttat tttcatcaaa tcgatcgatt cactattcca gaatgggtaa aagaaacagt
    30901 atggtatcaa atttttcccg aaagatttgc aaacggggac aaagcaaatg accccgaaca
    30961 cactttacct tggggaagta aagatccaga ccgagaagac tttttcgggg gagatttgca
    31021 aggtgtgatg gatcatttgg attacttgac ggaccttggc atcaatggca tctatttttg
    31081 tcccatattt aaagccacat ccaatcataa atacgatacg attgattatt atgaggtgga
    31141 tcccgctttt ggcgataaaa aacttttaaa aaacttgatt gatgaagcac ataaaagagg
    31201 gatacgtatc atgttcgatg ctgtattcaa tcatatgggt gtccattcac cacagtggca
    31261 agacgttctc aaaaacggag aaaaatcgat ctataaagac tggttccaca tccgtttttt
    31321 cccagtagat tcttatcaga tgactgactt accagaaact gctgaaaata tcccatatga
    31381 tacttttgct tttactccat tcatgccgaa actaaataca gcaaatccag aagtgcaaaa
    31441 atatttgctg gatatcgcaa cttattggat aaaagaattc gatattgatg gatggcgtct
    31501 tgatgttgct aatgaagtcg atcatcattt ttggaaaaaa ttcagagaag cagttacaga
    31561 aatcaaacca gacatctata tccttggaga gatttggcat tcctctcaag cttggctgca
    31621 aggggatgaa ttccatgcag tgatgaatta tgcatttaca gattcgatta aagattattt
    31681 tgcaaaaaag aaaatcacag cttcacaaat ggtcagtgga atgaatcatc agcaaatgct
    31741 ttacagagat caggtgaacg aaggaacatt caacttatta gattctcatg atactgccag
    31801 gatcttgacc ctttgccaag gtaacaaaga attgatgaag tctgtgatgg cctttatgtt
    31861 tcttcaaaaa gggtctcctt gtatctatta cgggacagaa atagggatga ctggtgaaga
    31921 tgacccggat tgccgcaaat gtatgatatg ggagaaggag gaacaagatc tagagctatt
    31981 cggttttata aaagaacttg tttctctaag gaaacagttg tctaagatta tttctgaagg
    32041 ttctactcag tggctaatag taaatgatcg tgaagataag ctctatttca cacggaaact
    32101 agagggacag atcatctatg tttactttaa tcagtcaaaa gaaccttggg tagtagaaca
    32161 ggaaaatgag gtgattctta gtcaaaattg tcagttgtta gaagacggga aagcagagat
    32221 acgacaatac ggatttttga tcatgtgttg aaaactgtta ccggtaacaa caagaaagcg
    32281 tttgcaaatg aagatttgtg cccattataa taagggtgta acagcacgtt gaatacaaag
    32341 gaggaaatga agatgaaaaa aagaagtgtg aaacagttgg gtttagggtt aatctcttta
    32401 ggaatcttag gcggattagc ggcttgtggc aatggtaact cttcagcaga aggggaagcc
    32461 gatgaaggaa aaacgttaac agtttccgtt gattctggat ataaagatta tataaacgaa
    32521 ataaaaaaca ctttcgaaaa agaaaacgat gtgaaaatca agctagtaga aaaggacatg
    32581 ttcgatcagt tggaatcatt agcgttagat gggcctgctg gaaagggacc tgacgttatg
    32641 atggcagcat atgaccggat cggtgcgtta ggacagcaag gacatctggc ggaagtaaaa
    32701 ctgggaaatg aagcagctta cgatgaaaca gacaaagccc aagtgactta tgatggaaaa
    32761 atctacggag aacctgcagt aatcgagacc cttgttttgt actataacaa agatttagtc
    32821 gatacagcac catcaacgtt taaagatttg gagaatctat ccaaagacag tcgttttgct
    32881 tttgagtcag aagcaggaaa aaatacagga tttttagcta aatggactga cttttattat
    32941 tcctatggtt taattgcagg ttacggtggc tatgtatttg gtgatgatgg cacaaatcct
    33001 tcagatatcg gtttgaataa tgcaggtgct gtagaaggaa tctcttatgc aacagattgg
    33061 ttcaaaaatg tttggccaaa gggaatgcaa gacataaaaa gtgcaggaga cttcgccagc
    33121 cagcaattca tgtccaataa aactgcagca atcattgacg gtccatggca agcgcaaaca
    33181 tacaaagaaa acaatatcaa ttatggtgta gccaaaattc ctactctaaa taatggacag
    33241 ccttatcaac cgtttggcgg aggaaaagga tgggtagtca gcaattatgc taaaaacaaa
    33301 gacctctctc aaaaatggct tgattatgta acgaatcagg aaaatcaaga aaaattcttt
    33361 gaaatggtaa atgaaatccc tgcaaaccaa caagctaggg aaacagcaaa ggctaaaaac
    33421 gatgaattaa caacagctgt gattgaacaa tatgaaacag ctcaagcgat gccaaacatc
    33481 ccagaaatgg gcgaagtttg gacaggtgca gaaaacttga tgttcgatgc agcatcaggt
    33541 tcaaaaacac caaaagaatc cgcagatgaa gcagtaaaaa caatcagtga agccatagaa
    33601 caaaaatact gattaaaatg ataaccgtcc atttttataa aaaatggaac aggtgcttca
    33661 gataaaagtg gcaagagcga gatggaaaat tatcttgctc ttgttgccat tatcttaaaa
    33721 gcagtttgcg gtttagcaag tttagagggg gcattcagat gcagcaagat aaaacaagca
    33781 atatcaacga aaaaaaaatc acgctttgtt cacttattcc agggatgggg cagtttgtaa
    33841 atggccaagc ttttaaaggt atcgtttttt tcgccatatt cgtcctattc attggtcaga
    33901 tgcttctggg cggatttcat gcattggtag gattggttac tttgggaagt ataccaatgg
    33961 aagatcattc attatttatt ttgattgaag gcactttgca attcatcatt acaatcattt
    34021 tcttgatttt ttacggtttg aatcttttag acgcttatcg agtagcgaag atgcgtcaag
    34081 agggcaaaaa aatcaatcgg acgatgaagg aagtcataca taatataggt gatgaaggtt
    34141 ttccttatct attgacactt cccgcttatc ttctcatgat ttttacgatc attttcccag
    34201 tcctagttac attgttcact gcatttacta attatgactt caatcatatt ccaccagcca
    34261 gtttaattga ttgggtaggg gctaaaaact tttttagtat cttcttttta agctcatatc
    34321 gtaatacttt ttttgcagtc ttttcgtgga ccgtgatttg gactttatgt gcaacaactt
    34381 tgcaaatcac ccttggtatt ctcacagcgg ttgttgctca ccagaaattt attaaattca
    34441 aacgtatttt cggtatcatt ttcttattac catgggcggt cccagctttt attactatca
    34501 tgagtttttc gaatatattc aacgatagcg taggtgccat caatactcaa gtgattcctt
    34561 tcataaacca gcttcctttt gtagatatca gtgcaatctc ttggaaaacg gacccgaact
    34621 ggacgaaagt agcaattatc ttagtgcagg gctggttagg atttccctat atctatgtga
    34681 tggttacagg aatcttacaa tcgatttcag aagacttata tgaagcagca aaaatcgacg
    34741 gagcaaatgc atggcagcgc ttttcaaaca tcacgcttcc tgctgtcttc ctagtggcag
    34801 cgccaacatt tattacacag tatacgggga atttcaataa tttctccatg atctatctgt
    34861 ttaacaatgg cggtcccgga agcgtaggcg gaggagctgg ttctactgat attcttattt
    34921 cctggatcta taaacttacg actggaactt ctccgcagta ttctatggct tcagcaatca
    34981 cgttgattat ctccgtcatc gttatctgtg tgtcattgct ggtatttaaa aagacaaatg
    35041 cgttcaatat gggggattaa caatgaaaaa atttcattcg agtgctcgat tccaaaaacg
    35101 actttctcac ttttttactt acttttttct tttaatactg tcagtcatta tcatctatcc
    35161 gcttttgatc acagcaagta ctgccttcaa gtcaggaaat attgcagctt ttagtttaga
    35221 tctttcaagt gactggactt taggaaactt tactcggcta ttcagtgaaa cactttacgg
    35281 gacttggtat aaaaatacat tgatcatttc tgtcctaaca atgatcgtac aagtgactgt
    35341 agtaactttg gcgggctata cttatagtcg ctatcgtttt atcggccgca aatcaagttt
    35401 gatgttcttt ttagtgattc agatggtacc gactatggca gcattgactg ccttttatgt
    35461 catggctttc ttattagaag cgctcgataa gtactggttc ttgacaatga tctatatagg
    35521 cggtggaatt ccaatgaata cttggctgat gaaaggatac tttgacactg taccaataga
    35581 tttagatgaa tctgctaaac ttgatggggc agggcatttc aggatttttt tccagattgt
    35641 attaccgctt gtcaaaccga tgctcgccgt tcaagcatta tgggctttta tgggaccttt
    35701 tggggatttc atgttagcta agttcctgct tcgaacacct gaaaatcaga ctgttgctgt
    35761 cggcttacaa acatttattt ctgatgtgac aaaccaaaga gtctctcttt ttgctgctgg
    35821 ggcaattttg attgcactac cgatttgtct attattcttc tttttacaaa agaactttgt
    35881 taccggcctg acatctggcg gaacaaaagg ataggagcag agtatcatgc aaacatttcc
    35941 gttaaactat ttttccgcat tacgtacgcc aactcaagtg tttgcgggac gcaaactatt
    36001 atcatggcct aaatttttct tgatatttgt atttttagtt tccttgatgg tcatgcctgt
    36061 gactcttttt tatgccaatc agatccaagc gattccatta gaacaatttc tatccgttca
    36121 ttcgctgatc gatgaacaag gaacccaaaa attttctgaa cttgaattat ctgaaactgg
    36181 gttacaagca tcgcaacaaa cgatagctgt gacccctgaa attttggtag gtgtgtcttt
    36241 atctgagaaa caacaatccg atcatggaac ttttattgat tttgagaagg aacaatgggt
    36301 gatccaacaa aaggataaat ctggtatacg ccgctatacg atgaattata gcccatcttt
    36361 ccaaccagat tctgtacgta cgcccgaaga tttccaacga tttttagaaa gagaatttta
    36421 tgccagtaat cgtccaacaa tcattttatc ttatagttta agtcttggtc ttgtcctatt
    36481 tgtcatgacg agtctcattt tattcggggc ttcctttttc ttgtggatga caagaaaaag
    36541 ccaactttca tccattcata cattcaaaga gagtgcaaat ttgatgctga atgttatggg
    36601 aataggttcc atggcagcag cagttgtagg gtttatccac tttgacttta tcttgatgct
    36661 aagtgtccaa acaacgataa ctgtcttgct gcttttatgg gtttttgtca aaacaagatt
    36721 caaagataag cgtgtcgaat gacaagttta agcgatttat gcgacaatga atgtgaaaaa
    36781 aatgaaaaat aaagtagttt ttgaaaaatt tctgaaaaaa ttaaacttct tagttgattc
    36841 ttttcttttt tagtattaac gtaatagggt agttttttta aggaggaaaa tcatgttaga
    36901 gaaaacttta tacaatcaat tattttcacg ttctttctcg ctccctgtcg aagtaaatta
    36961 ttgggacggt acaacgaaac aatacggaga tacggacact cctccccaaa tcaaaatcaa
    37021 tatccatgaa gaaatcccaa tgaaagagat tacaagtaac gcttctttgg cactaggaga
    37081 agcctatatg gatcatcgaa tcgagatcga gggaagtatc caagcattga tcaacgatgc
    37141 ttatcagcaa gcaaacagct ttttacgagg aaaagattat ctaaaatggc tgccaaagaa
    37201 agaaaaacat acgaaacaaa aaaacaaaga agatatccat gcgcattatg acttaggtaa
    37261 cgacttttat aaactttggc tagatccaac tttgacttat tcttgtgctt atttcaatac
    37321 acctgaagat acgttggaac aagcacaaat caataaagtc catcatatct tagacaaact
    37381 ctttatcaaa gaaggcgaaa cgttgcttga tatcggttgt ggctggggaa cattgatgtt
    37441 cactgctacc aaagagtata atgtcaaggc aactgggatc acactaagtg aagaacaata
    37501 cgatttgatt accaaaagaa taaaagaaga acatttagaa gataaatgcc gtgtattatt
    37561 aatggattac cgcgaactaa aaggcgaaac gtttgatcat atcacaagtg taggtatgtt
    37621 cgaacatgta ggttctgaaa atcttgaagg ttatttcaaa gtggtaaaag atttattgaa
    37681 accaaaagga acagctttga tccatggaat cagccgacaa caaggcggtg cgacaaatgc
    37741 ttggatcaat aaatatattt tccctggcgg ttatgtccct ggtgttactg aattagttga
    37801 taatattaca aaaaacgatt tgcaactgat tgatttggaa agcttacgtc gtgactacca
    37861 attgacatta gaacattgga caaagaattt tcatcaagtc caagatcaag tcacgaaaga
    37921 aatgggtgat tccttctatc gaatgtggga tctatattta caagcttgtg cagcttcatt
    37981 tgaatcaagt aatatcgatg tgattcaata tttgcttgtt caacctggaa acaatgacat
    38041 cccaatgcat cgtgcataat tgaggcaaaa gtactgttta aagactattt aaaaagaagc
    38101 taagttttat gatgctccag aagtgaactt ttttgtctaa cttctaggat tgtctctaaa
    38161 gcttagtttc tttttcttta tattttcata ataaaagttt ggttctttaa tttttaaaca
    38221 tgacagaaaa ttttttcttc gttatacttg taatagaata ctattttata acaaagggga
    38281 tcgaatcaat gaaaaaaact gttatttata ctagtttatt tgcagctttg tttttagctg
    38341 cttgtcaatc aaataccaat caaggcacat ctgggaatac gtcttccagt agcgaacagc
    38401 gctcttcaca ggtatcggac tcctcaacaa aagcaagcac atcttcgtct agccagacaa
    38461 aaagttctag tgaagcaagt actcagtcag atatcaaagt gtctgcagct cgtgcgatcg
    38521 aattgttcca agataaatac ccagatactg caattactag tttggaatta gatagtgatt
    38581 ggggatctta cttctacaag atagaaggtg tcgatgatca aaaagaatac gaagcaagaa
    38641 ttgatgctca aaatgaaaaa atggagacag agaatccaga aactctagat cgagatgaac
    38701 aaaatggtca aaagaaaaaa gaagatggtc tggatgttga taaattgatc acgattgagg
    38761 aagcagcaaa aatcgccgtc gacaaagtag gaagcggtac ggcaacagat tgggatttgg
    38821 ataaagattt aggtactact tattgggaag tcaaagtaaa agacggcaat caaagtacat
    38881 cagtcaagat caatagccaa acaggcgaag tactgtcaac tgaacaagat gattaagaat
    38941 ttcttcggaa aacaagagtt gatttttctt ttagtcatga tacactagac aaaataactt
    39001 gtttgaagga gcgatttact tgagtttaga aacgaccttt gccaatcata tctttgccaa
    39061 tcctttgatg aatgcatctg gtgtccactg catgactacc caggaattag atgaactggc
    39121 tcattcagaa gcaggagcat ttatcactaa gagctgtacg atcaatgaac gaaaaggaaa
    39181 tcccgaacca cgctattttg atgttccttt agggagtatc aattctatgg gacttccaaa
    39241 cttaggtttt tcttattatc tagagtatgc tcttgcgtat gaaaaagtgc aggaaaacca
    39301 aaatcaaccg ttattttttt ccattgccgg gatgagtgtc caggaaaact tggaaatgct
    39361 tgagaaaata gaaaaaagtg ggtttaatgg gatcactgaa ttaaatttat cttgtcccaa
    39421 tgtgcctgga aaaccacaac tagcttacga ttttgaggcc acttatgaaa cactgaaaga
    39481 agtattcagc atcttttcta agccgctagg aatcaaattg cctccttatt ttgattttgc
    39541 tcattttgat cagatggctg atatcttgaa tcagtttcca ttgacctatg tcaatgctat
    39601 caacagtgtg ggcaatggtt tgtatatcga tacagaacaa gaagcagtag tcatcaaacc
    39661 aaaagaaggg ttcggcggca ttggcggaga gtatatcaag ccgactgctt tggcgaatgt
    39721 tcgcgctttt tatacacgtt tgaaaccaga aatccaaatc attggtacag gtgggattcg
    39781 gacaggacaa gatgcatttg aacatctgct atgtggcgca agcatgttac agattggtac
    39841 cgagcttcat aaagaagggc cggaaatttt ttctagaatc ataaaagaat taacgcagat
    39901 tatgtcagaa aaaggatata cgtcaattga cgaatttaaa ggcaaattac gcaccatttc
    39961 atagcaaaga gacgaaggtc atgattgatc ttcgtctttt tttataaaaa acactcgtct
    40021 gttcattcct gtgcaagcgt actcattgta tgttagacga acttgtggga aaataacaga
    40081 ggcaaaaagg aggataatat gaatgatgca acagaattag caaaagagct taaagaaaaa
    40141 gaatttagtt ttcaagaact agttacggat atacagaaaa aaataaagaa gcaaaatcat
    40201 gaattgaatg cgtttgtcgc gcttgaacca ttcgagagta attcccacga gctattgatt
    40261 ccccagtcag aaaaagggcc attggctggt atcccatttc ctttaaaaat gctcggtcaa
    40321 gaaaaaaagg gatggctagc tacttcagga tctcgtttat ttgaaacaca ccgagcttct
    40381 catacatcta attatgtgcg gcaagcagaa gcgataggtc tagttccttt tggacaaacc
    40441 aatgcgccag agtttggttt caaaaatatt acagacccag tcatctatgg cccagcaaga
    40501 aacccttgga accttgaatg tacaccgggc ggatcaagtg gaggagctgc tgctgcagtt
    40561 tcatcaggaa tcgttccttt agctggtgct agtgatggcg gtggttctat tagaataccc
    40621 gcttcgtttt gcggattgat tgggctgaaa ccttcgcgag ggacgatgcc agttggtcca
    40681 tatgcttgga gaggatggca aggagctgct attgatttcg gcttgacagt ttctatgaga
    40741 gacacggagg ctttatttta tgggatgaga agtatccatt ccggtgcgcc atatcaagta
    40801 tcgccagttg agtggcaaac acatccaaga aaaaaacggt tgaagatagc tttttgtgtt
    40861 gattcaccga tccactcaca gattagtgaa gaggcagtac ttgctgtaaa aaaagctgta
    40921 gatttccttg gaaaagaagg gcatgatttg acagagattc cttacccact agatggtcgt
    40981 aaactgatca cgtcttatta tcagatgaat ggcgcagaaa cagcagcaat gatccgctcg
    41041 attgaaacag gcatccagcg agctgtcaga aaagaagaac ttgaacccat ttcttggact
    41101 ttgttccaat atggtcagaa gctctctgcc gctacttaca tcgattctct gcatgtatgg
    41161 gatgaagcag caattgtcat ggaaaaccta catcaagaat acgatctatt cctttcacca
    41221 acgactgcat ttaccgcccc taaaatcagc gaagatttgc aaagtgatgc gataagagca
    41281 cagatggaac acatcagtga ttgttctgaa gtagaaggtt tagacgttgt ttcttccatg
    41341 tttgaggaga gcttgaagat cacaccttat acacagttgg ctaatctgac tggacaacca
    41401 gcaatcagct tgcctactca tttgacaaaa gaaaacatgc ctctaggaat ccaatttatg
    41461 gctgccagag gaagagaaga tttgctattt caaatcggct atctatttga agcaaatcat
    41521 cagttccatt taccaaatcc atggaaatag cagaaacaag gagaagaaga atgaagaaaa
    41581 aaagattgtt tgctctaata actagtgcct tttttatttt aagcgcttgc ggtacagctg
    41641 ccgctaatca ggaagataac agctggagta atgtagaaaa aaagaaagaa ttagtagtag
    41701 cgacttctgg cacactgttt cccgcatctt attataatga tcaaaatgaa ttagttggtt
    41761 atgatgtaga tttggcaaaa gaggtggcta aacggttaaa tgtcaaaatc tcctttaaag
    41821 aatacaacgt ggatggacaa atcacttctg tttcaaaaag agaatcagat tttgctgcga
    41881 acgattttgg tatcacaaaa gaaagagaaa aaaaattctc cctttcagag actttcaaat
    41941 attcatttga cagtatgatc gtacgaaaaa aagatgattc aggaatccac tcacttgaag
    42001 atcttaaagg gaaaaaagca gctggcgaac ccaatactag ctatatgcgt ttagcggaaa
    42061 aacacggagc taaattagtc acatatgaca atgccacgaa tgatcagtat ttaagcgatg
    42121 tagcaaatgg tcgaacagat gttattctaa atgattatta tctgcagaaa atggccgtat
    42181 ctgctttacc agatatccct gtaaaaatac tggagaacgt ttatttcaat gaaaacgaaa
    42241 gtggtttttt attgaaagaa ggaaatgatg ctctgaaaaa gaaaatcgat ggcgtattaa
    42301 aagagatgaa agaagatggg acgacaaaga aaatatctga aaaatatttt caaactgatg
    42361 tttcagtcaa accgaaagaa acgatggctg agtcggtgag tgagtgatgt actttcctct
    42421 aattgatttt tccctcatgt ggacttcact tcctttcgta ctgcaaggct tggtatacac
    42481 attagggatt ggcttcgtgt cgtttctttt aggaaatctg gtagggttgc ttttgacagt
    42541 tttaggtctt ttggattggc tgcctctgaa tgtatttatt cgtttttatt tatcgttctt
    42601 tagaggcatt cccgcactag tcctgctgtt tctgttatat tttgggttac cttatcaatt
    42661 aagtgcctta acggcttcag tcatctgctt tacgattacc agcagtgcat ttattggtga
    42721 aatctatcga ggatcactag caggcgtttc ttcaggtcag tgggacgctg gtttagcttc
    42781 tggtttttct ttctttgggg tattgaaata tatcgttttg ccgcaagctt ttcgaatctc
    42841 agtaccggca ctcagtaacg tagctatgga tctagtgaaa ggtacttcgt tagcagcaac
    42901 aatcacagtg ccagaaatat ttcaaaaagc aaagatcata ggcggtcgga cattcgatta
    42961 tttttctctc tatattttag ttgcactgat ttattgggtc atttgtatcg ggatcgaaaa
    43021 agtgcaacag tatttagaaa aacgttctat gcatcagtca atctgaaaaa tagaaatggt
    43081 gctagcgtgg ctggcaccat ttctattttt tgtttcttgt atattctgcc tagtatttta
    43141 aaaccatttc atgtaaaatg taaatcgaaa atatgttcgg aggtagaaaa catgaagacc
    43201 ccgctaacca tcgaaataga atcctcgtta taccaatatt gcatcgagca aggcggcttg
    43261 gttgtagaag aagtcacgat gcctgatgac caaggaattg tagatacgct tagctgcttg
    43321 acaaaatttg atggaagccg agaatggcga tgctatgagc tgaaagtgac aaaagcagat
    43381 tttcgctcta cagccaaact gtcatttgtt ggacattata attattttgt tttaccaagt
    43441 aaactatatg aagaagtaaa aactgaaatc cctacaggaa taggtgtttt agtgtatcgt
    43501 ccgtacacgg ttgaagagga gatgcctgcc tctggtacat ttctgattgc gaaaaaagca
    43561 gcaaagaaag agcttgctgt accggaagaa gctttgacaa accgcttcat ggcttcttta
    43621 ttcagggaag taagaaaagc aaagcaaatg gagcgaggcg taaaatattt tccaacagat
    43681 caattgtaca aagaattgaa aagacgttca gaaaatcaag atccattttc taacgaacga
    43741 tattatgaac ggtttttaga ggaaatagct agtcaggcaa tcaatgagtt gcaagaagaa
    43801 gttatcgcgc ttcagcatga ttatgaatat ttgaggcaac aacagcagat ccgtcggttg
    43861 cctacagagc cattggagtg aaaaatatgt actatcctta tttaagagga aaacaatttg
    43921 acctattagc attaaaagaa gcactgtcgc gaggattgct ctccaataaa atccaaccag
    43981 tgatcgaacc agtacgtgat tccgctacgc ttaaaaatgt gatcgaatta tttcaaaaaa
    44041 agcaccatcc aatcgctgtg atccaaaatc cccaagtagg acaattcaaa ttattcgatc
    44101 agcatgtgca cagttggaca gtcaaagaaa atagctctgt cgtccctgca cagatcttaa
    44161 cgccagaaaa tctcagtgaa gtactggatt cacctccagc gtttcttgtt ttcgatggac
    44221 agcactaccc aaaagataca gaggtgtgga aacagttggc aggagttgat tcgaaatttt
    44281 tgataccaga tactagtcgc tttcgcatct ggctgcccga aaacaaaatt gtgatccgtg
    44341 actcttttca aacgagaaag catgttgaaa gttatgcaga taaaacagat gactttttta
    44401 gtgatgacta tctttttttc cacgcagacg gatatattgg attttctgat ttcacgatcg
    44461 aaggtagccg ttatttcgat aaagggttcc ctagccgagc gcttgctatc catctaacgt
    44521 atattgatgc ttacggcaat atacgcgtga aacattttgt ctctgacagt aacgactctg
    44581 ctaaagacca agcattgaag tttcttgaag caggagataa aatgaaaaaa tggatcatgc
    44641 gtcaccatca tcaactcttg attacttcag gaatgattga attattggct ctttaccagc
    44701 aacaaaaatt ccctggactg ggagtgttga aaaagtggtc acttatgcat catttggaac
    44761 ttattagtca cctgctagat catccaaaag attggcttaa atattattca aaagttcagg
    44821 aactatatga agtgatccaa tgattagata atgtaaatga aaaaggaata gaaaagagga
    44881 aactggatgt atattcggca aacaaggaaa gaagaagtag aagaagtggt agaaatcatt
    44941 aatgccgcag cggatttcat tgcttcgcaa ggctctcctc aatggcaaaa cgggcagaaa
    45001 ccaacaaaag aaacgatttt gaacgatatt gaaagaaaag aaagctatgt attaatagtg
    45061 gaagatcaaa ttgctggaac agctgcatta gtctcaagcg tggatcccgt ttacacaaaa
    45121 atagatggta gttggaaaga atcaactggt ccttatttat cgattcatcg tgtcgcttta
    45181 gcagaaagta ctcgaggaaa aaaactagca aaaccgttct tagaacattt gttgacgatt
    45241 gcagccagca aaaatatcca agatatccgt atcgatactt atccagctaa tgaaccgatg
    45301 gaaaagacca tctattctgc tggattcact tatcaaggga tgattgaatt tccttttgaa
    45361 cacggcgaaa gaaaagcata tcaaataatc atcaattgac tataaaatca tctgagcaaa
    45421 gataaagaca ttaacactac tgaaaaaaac aatcatatga tagccaagag gttgtaacag
    45481 aagtggccag cttcaaaaaa caagaaagaa tttccgaaaa tgcttctcaa atttttgtgg
    45541 aattttagcg tattttacga aatagctgct ttctgaaact cccttcatcg agaggtcgtg
    45601 acatagcttt tgtcacgacc tctcggctta tcctacgagt attattcgat acatttatat
    45661 caataatcct ttttttattt tatctgcagc ttcttggaac gctccatcgt ttgacacgac
    45721 taaaagtgag gggatagctt tcaaacgttt caactgtgct aaatcttcta cataaaccgc
    45781 cagtctggct gaaatctgtt cagatgaatc tccacgtttt ttcaggcgcg attgcaatgt
    45841 ttctttagag atcgtaataa agataggacg gactaccgtc ccgaaatact catataaatg
    45901 ccaaaaacca tttgctgtca aaacggcgta acaatttcct actgtcagtt tctttttaat
    45961 ttctgcttta ctggaaccat atttatgttg agcatattgg tcccactctg caaactcatt
    46021 tcccttgatc atctgattga atgtattgtc agaaacaaaa taataatcct cttgatcttt
    46081 ttccccaggt cttggtgatc gattcgtgta agtaatgatc ttttgttccg gctgaaaaat
    46141 agcttctgcg agtgaagttt ttccagaacc agaaggaccg ataaatacat agcaataatt
    46201 cattgtcatt tctcctttga tgattgaaca agtgaaaaag ctttggaaaa tatttccaaa
    46261 gcttttaaaa gtaactttgc ttttttgtat attataccag caagccagtc acaatagcac
    46321 ttaaaaagct gactaaagtc gctgttagca aaattttcaa tccgtgtttt gcgacaagat
    46381 ttcctttttc ttcatttaat cccttcatcg caccagaaat gatcccgata gaagagaagt
    46441 tggcaaatga aacgaggaag acagatataa ttgctgttgt acgctcagta aaatggaaat
    46501 taccatttgc taattctgtc atagcaacaa attcatttgt aaccagttta gtcgccatga
    46561 tactaccggc atccactgct tctttccatg ggattccgct gatgaaggca agaggggcaa
    46621 agatgtaacc taggatctct tggaaagtga tgccgaatat cccgtggaag atagcattga
    46681 tcatggcaat caaagcaaca aaaccgataa gcatagcagc aaccgtgatc gccacatgga
    46741 aaccatcaag aatgtattct cccaacattt ggaaaaatgt ctgttctttg ttttcttcaa
    46801 tgatcagctc atcttctttt tcttctaata catagggatt tacaattgaa gccagaatat
    46861 aaccgccaaa taagttcaaa acaagggcag tgatcacata tttagaatcg atcagtgcca
    46921 tataagatcc gacgatcgac atagaaactg tagacatagc cgatacactc atggtgaaca
    46981 aacgtttctc agataggaag ggtaattcct tttttatgga aataaaaact tccgattgtc
    47041 ctaaaatagc agaagctact ccattataag attccaattt gcccatcccg ttgattttac
    47101 ttaatcctag accaacagct ttcataaata atggaagaat cttgatatag cgtagaatac
    47161 cgataatagc agaaataaat acaattggca tcaatacgga aagaaagaaa ggtgcacttc
    47221 ctttatttgc tacaccggca aaaacaaagt caacaccttg cccagcaaaa gcaagaagat
    47281 gatcaaacat ttttgccaat acagaaacaa ccgctgtacc aaaagttgta cgtaataata
    47341 cgaaaccaag aacaagctgt aaaacgaaca tcacaattag tggtttcaat ttgattgctt
    47401 ttttgttact gctgatcaaa aaagcaagcc ctaatgtgac aaataaacca ataataccta
    47461 ataaataact caaataatca ctcctattcc aaattccgtt ttattttacc tgttttgcaa
    47521 ataaaatact aatctttagt gaaattttca ttactttttt agagtgaaac gcattcattt
    47581 tccattagaa aataaagcga acaaaataaa aggtttaaaa agtgtaacgt cttagtaaag
    47641 tctataaaaa aacgaaacta tcgattaagc taatagtttc gttttcaaca tttataagta
    47701 aactttttct attggtaata tagattttcg gaagggtaga caggtgcgca ggtaatatcg
    47761 attcctagtt ttttcaatgt ttgttcatca tttttattga ccatcacagt agagtgggct
    47821 tgtgctccct caagttcagc tagtttatca taagcaactt gagcgattgg attcgttact
    47881 gcactgattg ataaagcgat caagacttca ttgatgctta gtgcatccaa agaggaatga
    47941 agtgtctttg atttcaatcc ttgtattgtt tcaagaacta atggtgacaa taaaggaatg
    48001 tcatctgcga tatgggctaa atgttttact gcatttaaga taactgccgc tgacgcatcc
    48061 atcaatgaac ttgtacgccc ggtaatgatg acttcatctg gtaattcgat tgcaatcaca
    48121 gcagaaggat cttttgtagg actttgcgct tgaatcttat ctgcatagtc atgggcaggg
    48181 actaccacgt ttcggtcttc aggacgtaaa ccgacttctt ccataatcag tttgattcga
    48241 ttaactgctt cttcgtcgat caatcctttt ttgaagtcgc attctgtttg gaaataacga
    48301 cggatgattt cttgtttaga ggcttcttgg acaacggtat catccacgat cccaaatcct
    48361 acacgattca cacccatgtc agtaggagat tgataaacag attctttacc agtaattttt
    48421 tcaataatac gtttgattac aggaaatgtc tcaacatccc gattatagtt cacagctact
    48481 ttattgtagg catcaaagtg gaaggaatcg atcatattga catcttttag atcgactgtg
    48541 gctgcttcat aagcaatgtt cagcggatgt ttcaacggaa cattccatac tgggaaagtt
    48601 tcgaacttgg agtaaccagc tgcatgtcct ttttggcttt catgatacag ttggttcaag
    48661 gaagtagcga gctttccact tcctgggcca ggagctgtca ctacaacgat tggtttagtt
    48721 gtttcaatat actcattttt tccaaaacca tcttctgaga cgattttttc tacgttgact
    48781 ggatagtctt cgatttctga gtgtttatag accttgatgt ttcgtctttc tagtttattg
    48841 ataaagactt tcgtggcagg ctgtccacta tagcgagtga taacgacact gttgattaat
    48901 aagccataac cacgtaattc atcgattaac cgcaagatat ccatatcata agtaatccca
    48961 taatcaccac ggatcttatt acgttcaata tctccagcat agacgcaaat cagtatttca
    49021 gcttgatctt tcaatttttg cagcaatttg attttcgcat cttcttcaaa tcctggaagt
    49081 acacgtttcg catgcttgtc gtcaactagc ttcccaccaa attctaaata taatttgtca
    49141 tagtggttca ctcgttccaa aatatacgct gactgctctt caatatattt ttctgaatca
    49201 aaaccaattt ttttcactag gttccctcct taagtactaa agcatttttt catttttttg
    49261 aggattcgtc aactggaaga gcgaaattcc tcaaaaaaac aaagaaaatg gtttaaacca
    49321 acttgtctag atagttattc attggtatga tagtttttga taaggaaggt gctacatgaa
    49381 aaaaagacag aaaaagaaaa acgcatataa acactatatc cgttcgattt ttaccggtta
    49441 tgaaagaatg cttgaagatc ccgaattgga acaattgacg tttacttatc taaacgaaga
    49501 aacgcagcta actcgtgatg agcatcagcg cattcatttc acaacaagag atctgcctag
    49561 taaataaaaa ataagataaa ggtcatcgga ttcgattctg tcctatcaga tggaaagact
    49621 agttgatcga aaaaaaggct gtaccaaatt ttggtgcagc ctgttttatg gagcattgcc
    49681 cgctcttttt tataacaatt ttttacagaa aatggtacta ttgaagtatt aataagtgaa
    49741 aaacagatga gtaagaggag gcaagtagta tgaaagagcc agatacaaat gcaaattatg
    49801 agccagacga actagaggag acgaatgcgg atcaggatac aggacaagaa tcgtcttctt
    49861 tttacgatat caaagaaaaa gcaagcatga atctttccca attattagaa gacttacagg
    49921 cttttgaaaa agctgtccgc gaagaagata tccctgagat ctaccgttta tacaatggcc
    49981 gattgaatga agaattaaaa agaagctcaa atgaaaatca cgagatcgat caattgctga
    50041 tgcgtaagat ccatgatcgt tttttacagg aatttccttt tatggaacaa gtagaaaaca
    50101 tctcgccaac gatgagctat tacaaaatcg gcacttatta ccatgatcgg cccacggtcg
    50161 gtattgatgc aagtctgcca gaaatttttg ttttgcctaa gatcgatgaa gagtgggaga
    50221 aatattccca accggagtca acggactatg acaaaaaaat caatgagcta gacgcaaagg
    50281 tgatcactgc tcagacagaa attgaacgtt tagatgaaca aataaaagaa atcaatcgac
    50341 aaatgactga tttgaacgac aataaaggat tcttgaatcg taaaaaaatc gaggaagaaa
    50401 ttcaagaatt agagaagaag aagcaagtac tatctaatga aaaattaggc tggcttcctt
    50461 atattgagac gcctgaaaca atccagcagc aaaaagaagc attgaagcaa gaagcaagag
    50521 cagaccagct tcgtgcagcc atcgtagaaa aagaacaaag gcaaatcaga cgctatttcg
    50581 gtagcaaaga gggattcggt caagcaatcc atgaattctt gatgaattat cttggcaatg
    50641 agaatcctaa taatcaatct aatgaaggag gttccgagta tgaatgaaca cgatgaagca
    50701 gaatggacaa aaaatgaaga acaaacagaa gatcatgaag aaacgaacac ttcattggaa
    50761 gaacaaacaa gtaatcctct ggatgacaca gaagaaatcc ctatgctaga ggaaacaaaa
    50821 atacacgaaa aagaaaaagt aactccgacg aatacgaata ctgcagaaaa cacccatgct
    50881 tacgaaataa ctgaaaatcc cgggtattta gaaggaaaag agcaagatca tcaagatatc
    50941 gtcagtttga ttgaaacagc ggaagcagaa ttagcggatt tacgtatgag aaaagatttg
    51001 atcgaatcag attttccaca attattagaa tcatatgatc gttttatatt gaatgaagaa
    51061 ccaatctcca cgatcggaaa gacaagcctg atcgaatact tcacgtatga attactgaaa
    51121 ccgttgaatg atattggtct ggaacggtca aattcagctt atgacacatg gaaaacaaga
    51181 catttttcga tcaattatac gttgaatgga caaaacgaat tagtcttttc ctttgtatta
    51241 ccgaccatcg atcaacgata ccaagtggaa tcgatgagtc tcctaaacgt atcaccagag
    51301 acaatggaaa tcaatgtcca agatgaccgg gtgctttctt tgatccgaca ctggtcagtg
    51361 gatcgagtat tctctgcagg gcagatcacg atattcaatc acaaattgaa tcaattatta
    51421 gcccatgcaa gaacacttgg ttttaccgtt aatcagacat tactagacaa tacgaagcca
    51481 cttcgtttga atcttcagag cgaatttgaa ttgacgaatg aagtattgga tgatattttt
    51541 atcgtggcaa tgaaaaatcc atcctacgat atggaaaaga tcaacgaaac tacttatcaa
    51601 gtacgattag ataaaggaca gtctttaacg ataacaacaa acgaaaaaga tcaaacagtc
    51661 ttgtcgatct catctggaga ctacccacga tctgtcattg atttctttat caattacgaa
    51721 ttcttggtcc cattaatggt gagaaagtcc tgattggata agttggatta ccagatgtca
    51781 ttcagataat tctgttccaa aggacttgcg gcagttatcc agaataattg tcacaagtct
    51841 tttctgtatg tactaaatgg acgtgaataa cttgctgatg aaggagggat ttcattagga
    51901 atgtccacaa ataaaatagc acagcttagc ttattgtctg ctgcttgtgt agcaggtagg
    51961 atcgcttttc aatttattcc gaatttccag ccaatgactg ctattttttt atttatagta
    52021 ctttacttaa gcttaaaaga tgcgttaatt gtcatgagtc tttctattgc gatcagcagt
    52081 ttttattttg gtgttggacc atgggtcatc ggccaatgga tcagctatac tgttgttctg
    52141 agtttgtttt caatcgtctg cttgagaaaa acagtgcaaa ggaatctatg gttaaaggga
    52201 gtctgttttt ttctggcggg catggtttac ggaataggta tgaccgtttt tgatacgctg
    52261 ttatatcgtt tgcctcaacc ttggatctat taccttcaag gggtgtcgtt tgatttgatg
    52321 cacagtatag gcaatgttgt gttctttctt gtttttctgc cagtcgtcaa acgtttttac
    52381 aatcattcag gaggaaaaaa tgaaaaaaat aatttatagt ttgcttgcaa tgagtgtcag
    52441 tatactcatt ttgacaagtt gcagtacaac ctatacacaa tcggcttcca accaaaagga
    52501 ggaagtaaaa acatcgcaaa aagcaacaat cacgttacaa gaaaatggtc aaaacttttc
    52561 ggaaaaagaa gtgttcttca aaaaaggcga tgatttactg agtattttga aacgaaattt
    52621 tgaagtcaaa gaagataacg gcttcatcac ttcgcttgaa gggcactcac aggatgagaa
    52681 aaatcaactc tattggatgt ttactgtgga cgggaaaagt gctactactg gtgccaaaga
    52741 gatcaaattg aatgatggac aatcggtcat tttcaatttg tctaaactat aagtttcata
    52801 aaaaactgtt aaagggcgaa tattgccata attcagtgag ttcagttgat gaaagaagga
    52861 aaataacatg gaaaatttta aaaaaagcgg aatgttttcc gtaattgctg ctctaggggc
    52921 taatattcta gttgctatca gcaaatttgt cggctatgca atcagtggca gtgccgccat
    52981 gctaaacgaa agcatccaca gtatcgttga ttgcagcaat cagattttgc tgttattcgg
    53041 tgataaacaa gccaataaag gacagagcga gttgcatcaa tttggtgaag gacgagcaaa
    53101 atactttttc agtacaatcg tcgcaatgat gcttttcttc ggcggtgggg cacttggggt
    53161 gatggaagct tttgaaaagt tgacgcatcc atcccatgaa gtgggtaatc cgatcattgt
    53221 tatagcaata ctgttatttg gtttggtaat cgaaggaagt tcgttacgcg tggcaatgaa
    53281 agaaatcaaa gaattgaata ctgaaaaact ttccacgatg cgctttttgc gcgaaagtcg
    53341 acacagtgaa atactgatta tttttacgga agacttgtgt gctgtaatcg gtttggtctt
    53401 ggctttagca ggaacaatat tgacttatgc aaccgggatt gctgcatttg atgcgatcag
    53461 cggattgctg atcggtttct tgctgatggg agccgccatt ttcctagcaa aagaatttta
    53521 tagtctgatc atcggtgaaa gcgtgacagc aagcgattta tcaaaaatca agttagcttt
    53581 tgaacgtcct gagatcagtc ggctgatcga tataaaaaca gttcatttaa gtccaacgga
    53641 gattttagtt gcagcaaaag cagatatcgt tggtaacaaa gagaatgaag cttactcggt
    53701 catcaatgat attgaaaaaa atatgcgtca gtctcttcct gacaagaaga tgtatatata
    53761 cattgaaaca gataaatatg acccaaatta ttctcgtaaa taaaacgttt attcatgaat
    53821 tgaagcaaaa atgagaaaaa tataatcttg tttttaaata aaacggctac aatttagcga
    53881 atttatgtta caatgagtcc gctgtttatt tgttgtaatt aaaggagata ttttaatgaa
    53941 caaaaagaaa agtgcgccat ggcttcaagc tttgcatgca gtgatgccac tttgtataag
    54001 ctatatacca gtaggattag cttgcggagt attgttgcaa aaagttggat ttgatcctct
    54061 cttatcagga ctgttatcga ttctggtctt ttccggtggc gcgcagtttt tagttgcatc
    54121 tatgcttacg acgcaagcat catttgcaac gacattattg atggttttct ttttagaatt
    54181 acgctataca ttattaggag caagtcttgc cggttttatg aaaaaagaaa agcggacgtt
    54241 tttggctgtc ttttctcaat cactgaatga tgaaaactat gcggtgaact acttaaagtt
    54301 ttcaactgac ccttcctgga ataaaagaaa ggctttatat gtcaattggt tctcaatgac
    54361 tgcatgggcc ggtagcaata tgatcggtaa ttttttcggc tcggtgattc gagtagatgc
    54421 tgaccttgtc cattttgctt tgacggccat gttcattttc atgtttatca tgcagatgaa
    54481 aagcgcacta ctgatcttta caggtttatt ctcaggcgta ttaggggtcg tattcatggt
    54541 attattccaa aatactttcg gattgattgc agcaacagtc attgcttcat ttgccggatt
    54601 tatgctggag aaggcattca aaaaagaaaa agctaagaaa ctaagagacc gcggtaaaga
    54661 tgtcaatgag ccgctcttca acttcccgga acaggaggcc aatcaccatg actaaactat
    54721 atttgctact tttagttgct tgtctgttca tcgtggcata tatcccgcgc ttgttcccga
    54781 tgctttattt tactcatcga aaagtgccat cctggttcag tgactggatg aaatatgtac
    54841 cggtggcatt atttgcggcg ttagctttta aagatgtttt cattactcat gaacatctgg
    54901 atattgcttg gaatatcaaa atagctgcaa tgattctagt agcaggcgtg gcttacaaaa
    54961 cacgttcgat ggcgttgtca gttttgacag gacttgcgtc tgtctttttg ctgtctatgc
    55021 tttaaactgt tttattttat taaaaacaga gattgcgaca taagttgttc aaactgatct
    55081 atccgaactg catttgcaac agtcaattct ggatgcaagt ttattcgtag ggataaaaaa
    55141 gattagtgtt ggcttatgat gcaagctctt ttttatttta ggaattagag agggatttgt
    55201 ccttctatga ttttttgcta taatagggaa gatgtttatt ttatgaaaga atagttgagg
    55261 gaatttaaat gaataaaaaa ttgatgcaag gaacattttg gctaaccttt gctaatcttc
    55321 tatgtaaagt tttaggggta gtctatttga ttccctggct aagtatgatg gggaacaatc
    55381 aagatggtat gcttgctacg acattgtata atgttggcta tcttccttat ggattgttcc
    55441 tgatgcttgg tacagtcgga tttccaaatg cgattgcaaa aaaagtagct gtagccacaa
    55501 aagaaggaga taacgctgct tgtcggctga tttttagaag tacgatcaat atcatgtttg
    55561 tcatcggtat cgcctctgct gcactgatgt atttatttgc tcctttacta gccggaatca
    55621 gtccgattgc taacgttaat aatgggatac tagctatcag aagtttatgc ccatctttga
    55681 ttgctatccc aattcttagt gcaatgagag gatatttcca aggaaaaaat gatctgcggc
    55741 cttatggaac ttctttgatt atcgaacagg ctgtgcgagt gatcgttatc ttggctggta
    55801 cttattactt gcgcgtattg actgacggaa ccatattaga agctgtattg atcagtacag
    55861 tcgcttcttt cttcggtggg ttagctgcga ttatccatat gtatatcgtc ggacgcagaa
    55921 aagacttctt cgaacttaaa gattttttga tttccagccg ttatttcgaa agagaaaacc
    55981 gatctgcctc tgtctctatc attcgggaaa cactaccgtt tatttttgtc ggctcagtca
    56041 ttactatcgt tcaaatgatc gaccaagtaa cgatgaaacc actgcttcac tttttccgtc
    56101 cagagatcgc agatcagcaa ctggaattct tattcagccg tgcttctgtc aatccaaata
    56161 aattgacatt gatcctgatc tccatggtag gaacagtagc tataagcagc ttgccaatat
    56221 taagcacgat gaagaaaaaa gatcggatac aaatcgaaaa aacagtagga gatagttttt
    56281 cgattgcgtt attgattctt cttccttcat tgactggaat gtctttatta gctggaccac
    56341 tatacacatt gttttttggc tatgatcctg aaagtgtcgg atatttccaa atggctttgc
    56401 tagcttcttt gtttttctct ttattcacga tcctatctac gatgcttcaa tctttgaatc
    56461 accatcgttt tgcgatccga ttaactgtag aagcaattat cctgaaagtc atcttccaag
    56521 taatcggatt aggattagtt ggcggatacg ggatgagctt atcaaatggc ttagcattcg
    56581 gtattgtctt tgttcgcggc tatcacttta tgtgcaaaga ataccgaata gcacctttag
    56641 caaagatcag caattttttc ttaaaaactt tccgctccac cctgattatg cttgcagtat
    56701 gttttgccgt tttctttttg ctgaatcagc gattgtcaat ggaatcaaaa agctatgcaa
    56761 tgatttattg tgtggctgta ggaagtattg gcggcttagt atttttattt tctcaattcg
    56821 gaaaaaatgg gtttcgaatg ttgaaaaatg ttaaacatca tagcagaagc aaagaatagg
    56881 ttaactaatc gttttttgct gtcacttgaa aaacggcaac tcgtatgatt acgcttgtcg
    56941 tttttctatt ttccaatttt agtttcatat ctaatcttac ttatgatata gttataaggt
    57001 ctggaaaaat tctttgaaac gaggaaatta aacacatgaa acttgcagta gttacagata
    57061 gttcggcata tctgccggaa cgtatcaaga accatcccga cttattcgtt attccaatcc
    57121 ctttgattat ggatgggaaa atttataacg aaggaatcga tatcgaagcg gatgaatatt
    57181 atggactttt aaaaaacagt aaagattttc ctactacttc acaacctgtc gtaggggaag
    57241 ttttgggttt atatgaggaa ttgaaagaga aagggtatga cacgatttta agtatccatc
    57301 tttcttcagg gatttctggc tttgtcaaca cattatttgc gatgaaagat gacatcagcg
    57361 gagtaacggt cattccttat gactctaaga tcactagtat gccgatggga cacatggtag
    57421 aagctgcctt agacttaaat gatgaaggtc agtcccttga tgaaatactt gcacatatcg
    57481 atcgaatccg agataatacc tatgcttatc tgattgtaga tgatttgaac aatttagtac
    57541 gaggaggacg tttgacaaat ggtgcagcgc tgattggcag cttattaaaa atcaaaccga
    57601 tcctgacatt ttctgaaggg aaaattgttt tattcgaaaa aatccgttca agtaaaaaag
    57661 catttgctcg agctgaagaa attattggta gaagaaataa cgaaatacaa ccgccagtca
    57721 aactttacgt gatacatgca aataatttag aagtagcgat tgcggagaaa gagaaattgc
    57781 agaaaaaata tccaaatgca gaaatcgaaa ttggtcattt cggaccagtc attggtacac
    57841 atttaggtga aaaagccatc ggcattgcca tttcagcgca ataattcctt ttattcaaaa
    57901 aacataccaa aaata
//
LOCUS       DAJSAW010000013        54786 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJSAW010000013 DAJSAW010000000
VERSION     DAJSAW010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.013"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..928
                     /locus_tag="ONT64_000874"
     CDS             <1..928
                     /locus_tag="ONT64_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
                     /protein_id="HCR2302308.1"
                     /translation="LLPQRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLL
                     RYYVDSTTHEIILSFLGKVQMEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIH
                     IEVPPNPFWASIGLSVAQLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYG
                     WNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQE
                     YLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTEL
                     KGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            1047..1214
                     /locus_tag="ONT64_000875"
     CDS             1047..1214
                     /locus_tag="ONT64_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2302309.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(1274..1627)
                     /locus_tag="ONT64_000876"
     CDS             complement(1274..1627)
                     /locus_tag="ONT64_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2302310.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            2132..2455
                     /locus_tag="ONT64_000877"
     CDS             2132..2455
                     /locus_tag="ONT64_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR2302311.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYLW
                     EFRYF"
     gene            2434..2763
                     /locus_tag="ONT64_000878"
     CDS             2434..2763
                     /locus_tag="ONT64_000878"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302312.1"
                     /translation="MGISLFLELPAIVSYWVVTVMCFVAMGVSTMFVVQIYTLVQTQT
                     PPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVASIASIMISIYSKR
                     IFQKLEI"
     gene            2990..3409
                     /locus_tag="ONT64_000879"
     CDS             2990..3409
                     /locus_tag="ONT64_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2302313.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDRKISELYHVSRSAICSRRTRGLRKLKMLLSEGK"
     gene            3414..3653
                     /locus_tag="ONT64_000880"
     CDS             3414..3653
                     /locus_tag="ONT64_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2302314.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            3911..4756
                     /locus_tag="ONT64_000881"
     CDS             3911..4756
                     /locus_tag="ONT64_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2302315.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            5037..6257
                     /locus_tag="ONT64_000882"
     CDS             5037..6257
                     /locus_tag="ONT64_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HCR2302316.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            6254..8974
                     /locus_tag="ONT64_000883"
     CDS             6254..8974
                     /locus_tag="ONT64_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2302317.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            8958..9902
                     /locus_tag="ONT64_000884"
     CDS             8958..9902
                     /locus_tag="ONT64_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR2302318.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(9971..10981)
                     /locus_tag="ONT64_000885"
     CDS             complement(9971..10981)
                     /locus_tag="ONT64_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR2302319.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(11161..11505)
                     /locus_tag="ONT64_000886"
     CDS             complement(11161..11505)
                     /locus_tag="ONT64_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCR2302320.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(11498..11932)
                     /locus_tag="ONT64_000887"
     CDS             complement(11498..11932)
                     /locus_tag="ONT64_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCR2302321.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            12070..12807
                     /locus_tag="ONT64_000888"
     CDS             12070..12807
                     /locus_tag="ONT64_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2302322.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            12800..14011
                     /locus_tag="ONT64_000889"
     CDS             12800..14011
                     /locus_tag="ONT64_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2302323.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            14215..14997
                     /locus_tag="ONT64_000890"
     CDS             14215..14997
                     /locus_tag="ONT64_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCR2302324.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            15067..15729
                     /gene="trmB"
                     /locus_tag="ONT64_000891"
     CDS             15067..15729
                     /gene="trmB"
                     /locus_tag="ONT64_000891"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR2302325.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(15827..17581)
                     /gene="ade"
                     /locus_tag="ONT64_000892"
     CDS             complement(15827..17581)
                     /gene="ade"
                     /locus_tag="ONT64_000892"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR2302326.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            17713..18642
                     /gene="tadA"
                     /locus_tag="ONT64_000893"
     CDS             17713..18642
                     /gene="tadA"
                     /locus_tag="ONT64_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HCR2302327.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            18599..19648
                     /locus_tag="ONT64_000894"
     CDS             18599..19648
                     /locus_tag="ONT64_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HCR2302328.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            19645..19923
                     /locus_tag="ONT64_000895"
     CDS             19645..19923
                     /locus_tag="ONT64_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR2302329.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            19898..20368
                     /locus_tag="ONT64_000896"
     CDS             19898..20368
                     /locus_tag="ONT64_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCR2302330.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            20385..20687
                     /locus_tag="ONT64_000897"
     CDS             20385..20687
                     /locus_tag="ONT64_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302331.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            20704..21075
                     /locus_tag="ONT64_000898"
     CDS             20704..21075
                     /locus_tag="ONT64_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HCR2302332.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            21047..21406
                     /locus_tag="ONT64_000899"
     CDS             21047..21406
                     /locus_tag="ONT64_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302333.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            21578..22585
                     /locus_tag="ONT64_000900"
     CDS             21578..22585
                     /locus_tag="ONT64_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR2302334.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            22610..23794
                     /locus_tag="ONT64_000901"
     CDS             22610..23794
                     /locus_tag="ONT64_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR2302335.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(24231..25814)
                     /locus_tag="ONT64_000902"
     CDS             complement(24231..25814)
                     /locus_tag="ONT64_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR2302336.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(26392..26535)
                     /locus_tag="ONT64_000903"
     CDS             complement(26392..26535)
                     /locus_tag="ONT64_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR2302337.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(26757..27236)
                     /locus_tag="ONT64_000904"
     CDS             complement(26757..27236)
                     /locus_tag="ONT64_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR2302338.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(27309..27593)
                     /locus_tag="ONT64_000905"
     CDS             complement(27309..27593)
                     /locus_tag="ONT64_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302339.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(27671..27865)
                     /gene="ssrS"
                     /locus_tag="ONT64_000906"
     ncRNA           complement(27671..27865)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ONT64_000906"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(27937..29223)
                     /locus_tag="ONT64_000907"
     CDS             complement(27937..29223)
                     /locus_tag="ONT64_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCR2302340.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            29361..30032
                     /locus_tag="ONT64_000908"
     CDS             29361..30032
                     /locus_tag="ONT64_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCR2302341.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            29998..30486
                     /locus_tag="ONT64_000909"
     CDS             29998..30486
                     /locus_tag="ONT64_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HCR2302342.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            30499..31446
                     /gene="prmA"
                     /locus_tag="ONT64_000910"
     CDS             30499..31446
                     /gene="prmA"
                     /locus_tag="ONT64_000910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR2302343.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            31447..32211
                     /locus_tag="ONT64_000911"
     CDS             31447..32211
                     /locus_tag="ONT64_000911"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR2302344.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            32444..34657
                     /locus_tag="ONT64_000912"
     CDS             32444..34657
                     /locus_tag="ONT64_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR2302345.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            34668..35114
                     /locus_tag="ONT64_000913"
     CDS             34668..35114
                     /locus_tag="ONT64_000913"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR2302346.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            35285..36544
                     /locus_tag="ONT64_000914"
     CDS             35285..36544
                     /locus_tag="ONT64_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HCR2302347.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(36633..37580)
                     /locus_tag="ONT64_000915"
     CDS             complement(36633..37580)
                     /locus_tag="ONT64_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR2302348.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            37974..39266
                     /locus_tag="ONT64_000916"
     CDS             37974..39266
                     /locus_tag="ONT64_000916"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR2302349.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            39281..41050
                     /gene="aspS"
                     /locus_tag="ONT64_000917"
     CDS             39281..41050
                     /gene="aspS"
                     /locus_tag="ONT64_000917"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR2302350.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            41159..42034
                     /locus_tag="ONT64_000918"
     CDS             41159..42034
                     /locus_tag="ONT64_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2302351.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            42300..44183
                     /locus_tag="ONT64_000919"
     CDS             42300..44183
                     /locus_tag="ONT64_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR2302352.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(44258..44542)
                     /locus_tag="ONT64_000920"
     CDS             complement(44258..44542)
                     /locus_tag="ONT64_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR2302353.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(44532..45266)
                     /locus_tag="ONT64_000921"
     CDS             complement(44532..45266)
                     /locus_tag="ONT64_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCR2302354.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            45395..46018
                     /locus_tag="ONT64_000922"
     CDS             45395..46018
                     /locus_tag="ONT64_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCR2302355.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            46210..47331
                     /locus_tag="ONT64_000923"
     CDS             46210..47331
                     /locus_tag="ONT64_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCR2302356.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            47338..50466
                     /locus_tag="ONT64_000924"
     CDS             47338..50466
                     /locus_tag="ONT64_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCR2302357.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            50485..53679
                     /locus_tag="ONT64_000925"
     CDS             50485..53679
                     /locus_tag="ONT64_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HCR2302358.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(53825..54532)
                     /locus_tag="ONT64_000926"
     CDS             complement(53825..54532)
                     /locus_tag="ONT64_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR2302359.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        17931 a         9503 c        11353 g        15999 t
ORIGIN      
        1 actattgcca cagagaaaaa agattgaaaa tccgcaccct ctactacaaa caactgttga
       61 accgagtaaa cctgaacaga gagaaatgtt gcttgatgcc cttttggaaa tctcagatag
      121 tgatccgctt ctacgatatt acgtggattc tacgacacat gaaattatac tttctttctt
      181 agggaaagta caaatggaag tgattagtgc actgttgcaa gaaaagtatc atgtggagat
      241 agaaataaaa gagcctacag tcatttatat ggaaagaccg ttaaaaaaag cagagtatac
      301 cattcacatc gaagttccac cgaatccttt ctgggcttcc attggtctat ctgtagcaca
      361 gcttccatta gggagcggag tacagtatga gagctcggtt tctcttggat acttaaatca
      421 atcgtttcaa aatgcagtta tggaggggat acgctatggc tgtgaacaag gattgtatgg
      481 ttggaatgtg acggactgta aaatctgttt taagtatggc ttatactata gccctgttag
      541 taccccagca gattttcgga tgcttgctcc tattgtattg gaacaagtct taaaaaaagc
      601 tggaacagaa ttgttagagc catatcttag ttttaaaatt tatgcgccac aggaatatct
      661 ttcacgagca tacaacgatg ctcctaaata ttgtgcgaac atcgtagaca ctcaattgaa
      721 aaataatgag gtcattctta gtggagaaat ccctgctcgg tgtattcaag aatatcgtag
      781 tgatttaact ttctttacaa atggacgtag tgtttgttta acagagttaa aagggtacca
      841 tgttactacc ggtgaacctg tttgccagcc ccgtcgtcca aatagtcgga tagataaagt
      901 acgatatatg ttcaataaaa taacttagtg tattttatgt tgttatataa atatggtttc
      961 ttgttaaata agatgaaata ttttttaata aagatttgaa ttaaagtgta aaggaggaga
     1021 tagttattat aaactacaag tggatattgt gtcctgtatg tggaaataaa acacgattaa
     1081 agataaggga agatactgaa ttaaaaaaat tccccctcta ttgtccgaaa tgcagacaag
     1141 aaaatttaat tgaaataaag cagttcaaag taactgtgat tacagagcca gacgcaaaga
     1201 cgcagagccg ataaaatgag attaatacaa tctcatttta tcggctcttt ccgttatgta
     1261 tggattcttt taattagtct tcgatgtttc ttgcttcgtt gataccgctg gctaaagatt
     1321 ccattaagga tagttctttg tctgtaaagc tatccatgta tttctctatc tgtaatcgtc
     1381 gggtgctttt taccaagtta ttagcaggta agaaaaattc atcaacggaa acatgaagta
     1441 acgatacaag gtcataaaga acttgtatgc tggggtgttg ccctttattt tcaatattag
     1501 ttaagtaccg tgggtcaatt tcaatcaatg ctcccacttg ttcacgagtt aaacctcgtt
     1561 tcaatcgagc ttctttaatg gctaaaccaa aggctctaaa atcatattta tcttcttttt
     1621 tacgcatagt agaccacctc tatacatttt attgttccta ctgaattaaa aacaggtata
     1681 gaaaaacgtg ttatatggtt tataggttta tatttaataa aaagcactac taaacgccaa
     1741 taaaaaaaac cgttatatgg tagtgctatt tacgctgtta aaatattgta tattacttcc
     1801 aaatggcggt ttgttggagg tcaacgtcgc catgaagtac atcatataca ataaatttcc
     1861 ttacattggg ttcttgtcaa aaaaagtcgt ctatctgcaa tagataagta cgtccaccaa
     1921 tgtggtttta taaatcatat agatagaata acagaagcat gtaaacagag aaataaatct
     1981 gtttatatgc ttttttggct attcagaact tttttacaaa gtttatttat cagtaatgca
     2041 acaaatcccc ctttcacatt gggactaaga gtgaaaggag ataaacgagc aaggctcact
     2101 tcctttccta gacagaaagg gggtgagaaa catgaaacca tcttcttttc agaccacaat
     2161 agaaaatcag tttgactata tctgtaaacg tgctatggaa gacgagcgaa agaattatat
     2221 gctttatctt tcaaggattg caaagcgtga ggtgtccttt tcggatgttg gcgattatct
     2281 tgttagccag tttgcgacaa cagataacta ttcaactgac tttcagattt ttacactcaa
     2341 tgggttatca gtaggcgttg aaaatgattt gttgagtgaa gcattacgtg agttgccaga
     2401 caagaaacgt gaaattctac tgctgtttta cttatgggaa tttcgctatt tttagaattg
     2461 cctgcaatag tttcctactg ggtagttaca gtgatgtgct ttgtggctat gggtgtatct
     2521 actatgtttg ttgttcaaat ttacactctt gttcagacac aaacaccacc gcaattagta
     2581 ggaaagataa tggctacgct ggtagcagtt gctatgtgcg gtcaacctat tggacaaatg
     2641 gtttacggta ttttattcga tgtatttata caagaaacat gggttgttat gtttgtagct
     2701 tctattgctt caatcatgat ttcaatatat tcaaaaagaa tttttcaaaa gttagagata
     2761 taatacaagg ggttaaatag tatgttaggc taaaaacaat atataaaaga aaatttgaaa
     2821 tctgctaaaa ggatagtgtt ataaaagaaa gagtaatatt gcagtattta aggctcgcac
     2881 aaatatatgt tttgtgtggg cttttttctt accatttttc tgtatttcta aagggtataa
     2941 acggtattcc gtttatatat ttcagcatta gcagaaaggg ggtgagatta tgaggacatc
     3001 ttctttcgag catatcgtca gattacagtt caattcttta atgctgattg tcattaagaa
     3061 caaagtgaaa agtcgaaaca gacagcttgc aagacattct aaacgtgaag tattgttttg
     3121 tgaaatagcc gaaacgaaac aagttgaacg tggaacgatt gatacctgtt tttatgattg
     3181 tgtatcattt aaagtcttgc actttacaat ttatgtatct gatgaaacac ttggtacagt
     3241 tttacataga ctgtcagaaa aacagcgtag tgcgatttta ttacgttatt ttcaaggaat
     3301 gaacgacaga aaaatttcag aactgtacca tgtatcacgt tctgctattt gcagtagaag
     3361 aactagagga ctaaggaagt taaaaatgct tttgagcgaa gggaagtaaa gaaatgagta
     3421 aagattatgg ctatccctct tttgaattga tttgcaaagc gtcaagcgga gatgaaatgg
     3481 cagttaaaga gattttgaag ttttatgacg gttatatttc taaattatgc ttgcgtcctt
     3541 tttaccactc tgaaagtggt aaaatcatta tgcaggttga tgaagaacta aaaggagaaa
     3601 ttcatacaga catgatgaaa gcaatattga aatttgaaat cggagtgaag taatcaactg
     3661 tataaaaaaa tcgggagata cacagtttcg tgctatctcc cttagtagtt ttacggtcat
     3721 atcaaggctc attcaatcgt ggtaaattcg tggtaagatg agtgtttttc tatacttcaa
     3781 aatgcttgaa tgtatagtgt ttatgcggat aattgaaaat tagatataaa atttcttaca
     3841 attagtatat gttttgtgag aaatttagac ttacaataag cagtgaattt ataatcaacg
     3901 tataggagac atggatgaaa tgaaactaat tggtatagat ttagacggta ctcttttgaa
     3961 ttctgaccag agcattagtc aaaaaaatgc aagaacaatg aaacatttac cggaagactg
     4021 ttttccattc atttgttctg gaagagaagt agaagacatt aataatattc tcaaaaaatc
     4081 tggtttaagg ataccagcag tagggttaaa tggttctgtt ggatttgatg gagaaaaaaa
     4141 gatatttgag tcttccttcg ataaaaatga tgtaaaaaaa attgattctg tcctatcgaa
     4201 atttccaacc aaaatatata caaatctcgg aagttatgaa tctcagaatt ataaaaataa
     4261 aatgaaaaaa atttttcacg aggtagggag tgaattttct atcaaagagc taaactatga
     4321 gcttgagtat gaaaaatcta tttgttcaat cccttataat aatattgagg aaattataga
     4381 aaaggatgct atcagcattt acaaattttt tatttttatt cctaatcgtc aattgaaagc
     4441 taatatattt aaccatttga aaaagatgct aaatgtgtct gttactgaat cggcacctgt
     4501 aaatattgaa atcgtacctc aaaatgtatc taaaggtgtg gtgtttgatc gtttggaatc
     4561 aatctacaat ttaaagaaac ctttgagaat cgcgatcgga gatagtttaa atgacttatc
     4621 tatgtttgaa agtgcagata ttagttttgc aatgggaaat agtcatcaag aaatcatgaa
     4681 aaaagctgat tatgtcactg tttcaaatga taaagacgga gtatctcaag catttgaaag
     4741 aattcttacc atataaacta attaaggtag tatagaacat agaattctaa atacggaaaa
     4801 atggagcata aattccagta caaatagtac ttcttcagtg tcaaacgaag cgaatataac
     4861 atagatattt aggattgtga caaaaatcat gtcacaatct ttttttcgaa taaacttatt
     4921 ttttgaagtc ggccatttat cgtaatttct ttgcataata cgaacaaatt ttcggttatt
     4981 gatgaaaaaa aacattgaaa atggtatgat aaaagaaaaa aaggaggaaa tttttaatgg
     5041 tgaaatttat ccatgcggca gatttacata tggatcggtc atttgaagga ctgacaactc
     5101 ttgataaaac tgtacaggaa aaactattga agacgaatct gaccgtttta tcaaatatca
     5161 ttgaacaggc aataataaat aacgttgatt ttgttttact tgcaggagat aattttcatc
     5221 aaaatcgtcc ttcattaaaa atacagaaac atttttcaga acagatgaaa cggttagaaa
     5281 agaaccatat acctgtattt ataatattcg gaaatcatga tttctatcaa aaagagcggt
     5341 attggttctc ctttcctaaa aatgtccatt tatttacatc cgaaacggta gagacaaaaa
     5401 aaataacgat aaaaagtgga gaaactgtct ctctcagtgg atttagttat cgtcaaccgt
     5461 ggattcaaaa agacaaagtg atggaatttc cttctagaga actcacagat taccatattg
     5521 ggttgtacca cggggaacct ggcgtctctc aaaaaggaaa ctatgctcct ttccaaccct
     5581 caaagatgca agaaaaggga tacgattatt gggctttagg gcatatccat gtgccaacgg
     5641 tcttaaatga aaagcaaaca attgtttatc caggtgcgcc tcaaggtcat actaaaaagg
     5701 aacaagcttc tacatctatt ctactagttg aattaagtaa aggaagttgc caaatacatc
     5761 cgattaaagt agcagaggtc tattggaaga caaaggaaat atcattgagg aaagcaagaa
     5821 cgacaaaaga ggtcattgtt cacattcgtc agcagctttc aaaagtagag gatggattta
     5881 gtttaattga gataaaatta aaagattatg accatctcaa tacagatgtt ttggaacgga
     5941 tacaatcagg agagctatta gactatttgt tagaggaatt ctcagatagg ataaatgatt
     6001 tctttatttg gcgtatttct ctcatagaga atacttttta tacgaagacc ccacttgctg
     6061 cttctgctaa attgatggaa cagttatttc aatactatca gacacctcaa gatttccaac
     6121 aaattttaaa tgaagtatat agtcatcaag aagcggctag aatattgagc gaactgccgg
     6181 aatatcaaga agaaacactt gaaaaagcca agcagctgct taatcaagat tttttatttg
     6241 aggaggatca ggaatgaaga ttttaaggat cgagattgct gcttttggaa aatggcgtca
     6301 aaaaagtttt gacttttatt caggcaatca gctgatctat gggggaaacg aagcaggaaa
     6361 atcaacgatt tatcaattta ttcaagcaat attatttggt ttcccttcaa aaggccgtaa
     6421 aaaaaaggat tatacaccaa aagacggctc agcctatggc ggcaaaatat ggcttaaaca
     6481 tcctgtatat ggtgaatttg ctgtcgagcg ctacaagcag caaaatcgcg gaaaatcgaa
     6541 ggtgtggctg ggggatcaag tgggaagtga tgagttacta gaaaaactga tcgcaccttt
     6601 gacgaaagag ttattccagc aggtatttac tttccagcaa gagcagttga tggaactaga
     6661 tcatttgaga gaaaaagagc tacatgatgc gctgatttct ttaggcatca cgggcagttc
     6721 tcaagtattc caaaagcggc aagaatatta tcagaaagca caacaattat tcaagaaaaa
     6781 aggccaaaaa ttactaatca atcaaaaatt aaatgaatgg aaagagctgc aagaaaaaat
     6841 tcatcaaaaa caagaacagg aagcttattt tggcaagttg atccaacaag aacaggaata
     6901 cgagtcgcaa cagcagcagt tattggcaca attaaaagag gcgaacaaac aattttcatt
     6961 agccagacag caagaactta atttttcttt atatgaagaa tggcaatctc tcaaaaaaaa
     7021 cagcactaca ggtcttcctg atgagcaact tcttttagat ctggagtctt tctccaaacg
     7081 gtatcagcaa acaaatgagc gtttggaaga attagaagca acactggaaa agcatagcgg
     7141 gatggatcag cagtcagcca gatattattt ttatttagag caggagcagg aactaaaaga
     7201 tctccagcaa cttcaattta cagcggaaca attaaacgaa gaatggcaaa gattacaaat
     7261 tgagactaga gaaacaaatt cagaattgaa gattttggaa caaaggtggc attggcagaa
     7321 aaatccgtta ccggaagaat ttttatatga agaagaatgg aagcatcttc tggctgaaga
     7381 aagaaggatt gctgatctag aaagccagat ttctgtgcgt ctccaaatag ctaaagaaca
     7441 gcaagagatg attgaagcag aagtgaatga cttggaagag acctatccag cattattaca
     7501 aaatcagtca gagatagcac cagtctcttc atctaaaccg tatatgctga tggctggcgg
     7561 agcaataatc attgcaggta tatttgtacc aatgcccttg aaagtgatcc ttttactaat
     7621 tggtgcaggc atgattggct atttcttcta caaaaggcaa ccaaaaatta aaactaaaaa
     7681 tgatttctct gagataaaag gaatgtggca agaaaaacta ggacaacttg atctagcctt
     7741 agctcaatta gctgaagcag aagaaaaaca aaagagaact ggagaagaaa aacagattct
     7801 tcagcaagaa gtatatcgtt ttgcggatca tcggcactta ggaaagatgg atacgctcaa
     7861 actcatgaaa gaatatggac aggaagtcat agattatcag aaattatatg gcgtatttga
     7921 gcggaaacat aaaagagaac aggaaataca agaaaaacta cgacaaatcg accaaaaatt
     7981 tgagtttgtt aaagaatggt taccgataca agataagaca atttcagaaa aaatggtatt
     8041 gctttcccgt tttatacaag agatggaaca gatgaaattt gccagaagct atcagcaaaa
     8101 tactttattg aagcaagaaa tcaatcaatt gaaaaagaaa caaaaagtat ggatacaaga
     8161 atacagcgaa caattgactc aagcaggctt atcttatcct tcggaagtac ctgtcttttt
     8221 acaaaaagcc aaacaagccg aacagcagat gaaaagatac aatgaattat cggaaatgct
     8281 tactccacta tttcctgaag agctgacaaa agagaaatta tctgtaagac tgcgccaact
     8341 gaaagaaaag caatcccaac tccagcaaga agaaaaagaa gcaagtgaaa atcggcaacg
     8401 actccaactt caagtagaag agttgcaaag agacggcaca ttggacgaac tttatcaaca
     8461 agctagccgg caaaaagcaa aattacagga attactggag gaatggggag cacttgaaat
     8521 cgccgggaca ttcttaggtg atctttcaac agagatgagt gaaagacaat taccccagtt
     8581 gttaagagca gcaggtgatt atttaggtat tttaacaaaa ggaaattatc agaagatatt
     8641 actgaaaaat gatacactga ctttaacaga cggatataca gaatttccca tctatgagtt
     8701 atctacaggt acgaaagatc agctgattat ggcgatgaga tttgcttacc tctcattaga
     8761 agctaaccgt tcgatatgcc cgatcatcat tgatgatggc tggcttcatt atgatcatca
     8821 gcgaaagtat caattagctg aacttttagc taaatttgga gaaaagtatc aaataatttg
     8881 tttttcttct gataaagaaa tggtaagcta ttaccatgag atgaaccaaa tcgtaaaaca
     8941 attagaagga gcaaataatg aaaaaaattc gtgatttagt aatcgatgag cagtttgaac
     9001 aatttgtcct gatcaaaagt gcagatgtaa gaatcgctaa aaacggaaaa aaatttattg
     9061 cttttacttt ccaagatact tcaggtacta tcgatggtaa atactgggat gcttcggagg
     9121 aggaaatcaa taaatttaca gccggcacag tcgtttttct aaacggtaaa agagaggttt
     9181 atcaaggaaa ccctcaagta aagattcttc atatgcgctt aacaagggta gatgagccag
     9241 gagagcctag tttatacatg gaaagagccc ctttgaagaa agaagaaatg gaagaagaaa
     9301 tcaatcaatt gttgtttgag atcaccaatg cgcattggaa tcgtattgtt cgctatctac
     9361 ttaataaata tcaaaaacaa ttttttgagt ttccggcagc aaaaagaaac catcatgcat
     9421 ttgccagtgg attagcttat catactttga caatgcttca cttagggaaa tcaatcgccg
     9481 atgaatatcc tgagctgaat cgagcattgt tgtatgctgg tatcattttg catgatttag
     9541 gaaaggtgat tgaactttcc ggtcctatgt cgacagaata tacgcttgca ggaaatttga
     9601 tcggtcatct ggtattgatt gacgaagaaa taactaaagc ctgtcttgcg ttgaaaatca
     9661 gcgaagctga cgaagatgtt cttgtattac gtcatatggt tttagcacac catggcttat
     9721 tagaatatgg ctcacctgta cgtccaagaa taatggaagc agaaatatta catcaaatcg
     9781 acaatatcga tgcttcgatg cagatgatgt tgacttcgat tcgtcaaaca gaaccaggaa
     9841 aatacaccga tcggattttc ggtatggaca accgaagttt ctatgtacca aaagatttat
     9901 agcaaaaatt tatttttttc attcaaaaca aaaaaagcga gacataagtc ttcgcttttt
     9961 ttgttttctt ttattttgat gaatcagttg tttcttcagt tgaagattct tttgttgaat
    10021 cagttgattt tgtgtcagaa ctctttgttg aagctgtagt ttcacttgag tctttcgtac
    10081 ttgatgaagt tgttgtgaat gcactcaaca cattttcaaa ggcatcatct ttgattttta
    10141 catttgcatc ttttaattct tcaccgatta ctttagttgt gaatgcattg tcacttagtt
    10201 tagtttcagt agcgatatct tttagttgat ctttgtattt atccatgtca ttgcctttat
    10261 tttggttttt caccattttg acaacgtagt attctgtcgc ataagaagta ggatttgttg
    10321 tagtaataac atcggagatc tcaccgtctt tcaatttgaa agctgcttct tttacttctg
    10381 caggaatagt tgttgtcgtt gagtcgaatt taactttacc gccatcttct tttgtctctg
    10441 tatcagttga tttgtctttg gctaatttag agaaatcatc tccatcatcc gctgattttt
    10501 tcacatcttt tgcttcatct tcacttgata atttgatgat ttgtgcttct acttctggat
    10561 ggaaagattt ccaagcagtt ttcaaatcgt catctgtaat atctacgtga gctttcaagc
    10621 cagcttcaaa agctaaattg ttacgaatat attctttata agtatctttc gtatatccag
    10681 cagcttctaa ttgtgattca aaagtatcac ctaatgattt cgcttgtttg tcgtattctg
    10741 catccacttg cttatcggtt actttatcgc catatttgtt attgaatact ttataaatga
    10801 tcatccgttg tactaaggat tggttcgatg attcaagttt tgcttcatca tagaaatcag
    10861 atacagtgat cgttccgcct ttcatcgttg cgatatcttt attagtatcg cctgaacaag
    10921 ctgctagagt taaaacagcc aacgcactag tagctgccaa aataattgat ttttttttca
    10981 ttaaaatttg cactcctgta attagttatt atttttatat attaactata ccatatccca
    11041 tttcctttaa aaacgttgtc gtggtagttt caaacaaaat tcaaaatttc atcacattcc
    11101 taggaaatac gcaaactttt ctcacttata tagttaaatc tgttgagaaa ataatcatta
    11161 ttatatttgt tcaggatact ctggcagatc agcttttagt ttttctactt gttcattgat
    11221 ttgtgtgata cgtggttcgg cttggaattt aaaatcttcg atatcttttt tgattgcttc
    11281 ttggaaagca ggaatcacag attcagctgt ttcttttgtt tcgatcaatg cgcttttaaa
    11341 atgattcagg ctgttattta aatcatttgt cagagttgtt gcttcttcca attcttcgat
    11401 cagtttcttt cttgtttcat tcccgctcct gggcgccaat aacagacctc cgacagtgcc
    11461 agcagcagct ccaaataaca gtcctttgaa aaagttttta atcatcgcag gacacctgct
    11521 ttacgattgt ttcttgaata gcttttagtt cttccgatcc ataactatct tgatgattga
    11581 caaagtgaat agaaaaatca tcctctttgc tgtagcgagg gactaaatgg atatgtgaat
    11641 gaaaaacaga ttgataggct agttcacggt tgttattcaa gatattaaga cctttcattt
    11701 ctggaaaagc tttttccaat gcttgagcaa tcttaggcac acgcgaaaaa acctctcctg
    11761 caagttcggg atcatattca aaaatatctg ctacatgacg tttaggcaca actaatgtgt
    11821 gtcctttcgt tgcttgagag atgtctaaaa aagcatagac cttgtcgtct tcatatattt
    11881 tatagcttgg gatttcttga ttgatgattt tgcaaaaaat acagttttcc atataaaaca
    11941 tcctttctat aaagaataaa tacttcagct taactttacc ataagttaaa gctattgtaa
    12001 ggaaaaaaac aaaataccgt cattttcttc gtggtataat aaataaaaat aaatggcgga
    12061 ggaaaaagaa tgagtttaat tatcgatcat gtcacagggg ggtacggaca tctccctgtg
    12121 ttaaaagata tcagtttcga tgtacagcct ggagagatag tagggttgat tggactgaac
    12181 ggagcaggga aaagtacgac catcaagcac attatcggtc tgctggaagc ttcaaaagga
    12241 acgatagaaa tcaatcatct atccttaaag aaaaatacgg aagaatatcg caaacaaatt
    12301 gggtatattc cagagactcc ttctttatat gaagaattga cattgagaga acatatcgaa
    12361 gtcactgcga tggcctacga tatacctatg gatatagcaa tgaaaagagc agaaaagttg
    12421 ttggagactt ttcggttatc taatcgtctt gactggttcc cagctaattt ttcaaaggga
    12481 atgaaacaga aagtgatgat cttatgcgcc tttttgatcg agccttcttt atatatcatc
    12541 gacgaaccat ttttaggctt ggatccacta gctatcaatg cattgcttga attgatggta
    12601 gaaatgaaaa aagaaggagc cggtattttg atgtctacgc atattttagc tactgcagaa
    12661 aaatattgtg atcgttttgt ggtcttacat aacggagaga tacgagcaga cggttctctt
    12721 gagatgttga gaaaagaatt tcagctgccg gattcttctt tggacgaaat ctatattgct
    12781 ttaacaaagg agcaggcaca tgagtagttt ttttcaaaaa cgtttagcaa gacatcaaaa
    12841 gaaaatgatg aaatatatgc gttatgtatt gaacgatcat tttgttttag tttgtctttt
    12901 cttgattggc ggtgtgggat tatattattc taattggttg aaaaccctgc agccaccgtt
    12961 tgcaattgga gggctcataa tctctgtatt ttggatgctc tgtttattcg tgggtaagat
    13021 ggctactttt gcagaggcag cagatattgt ttttttactt cctaaagaaa aagaaatgca
    13081 aacctatttg gaacgagctt ttcgctattc ttgcattttt ccgttgatct ttttgatatt
    13141 ggcatgcgga ttggccatgc cattagtggt ggttagtacg gagcaatcct tttcgatgtt
    13201 tttcgtctat ctgatcatat tatggagttt gaaattcagt catctgcaaa tcaagagagc
    13261 agaattgttt cgactagatc aacaagttat tcgttattgg aaattaggct ggtttttttc
    13321 ttctctgatc tttttgtttc tatcatttta ttggctaata tgggcaggaa tagcaggtgc
    13381 attagtacag gcaagtctat attactggtt tttgtggaag caacaagaaa aaggattgga
    13441 ttgggagaaa atggtgcatg tagaagaaca taggttgcat cagatctatc aatttatcaa
    13501 tttattcaca gatgttcctg aaattacggc aaagatcaaa agaagacgct atttggatcc
    13561 tatattgact catattaaaa gagaatcaaa aaacacttat ctttacttgt ttgcaagaag
    13621 attggcaaga ggttctgatt ttagtggtct ttatttccga ttagtaatta taggtgggtt
    13681 attgattgcc ggagtagtcg acttttattt tagtctgatt atcggtttct tatttttata
    13741 tttgatcggt ttccaactgt tacctttagc gaaccagttc cgctatatgg ttctcgttca
    13801 gctttatcca atccataaga atcagaagaa gcaggcgatt cagtatctac tcacatgggt
    13861 attaattatc gcgtcggtta tttttggttt gattgctgca gtcgtattag atgggcagca
    13921 gcgatgggtt ccgctagcgg cttatttgat tgttactatg ctctttgtta aactatatgt
    13981 accaactcgt ttgaagaaga tgtcagactg aaaacaaagc tgtgtagggt aaaaatgaga
    14041 atgaaaaata gagcgttgac agtaagaatg ctgttcgttc tatttttttt gagaaaagct
    14101 ggttttttga atggcaatta aatgaaaaag aacgtatatg tgcttgacgt gtagtgttca
    14161 ggcgactaga ataataagta ctgcaaaaca aagagtaaga ggacgggaga atgcatgatg
    14221 gagtttcagt tagaccaaga gtggcggtta cgtcccatca aaggcgacac aggaaaaaca
    14281 tacataggca tgcgtgatga ggacaaagtc tttattaaac gaaacacgac tccgatgttg
    14341 gctgctcttt caagggaagg aattacacca aaacttgttt ggacaaaacg aacggggaat
    14401 ggcgatactt tgacagcaca agagtggctg gaaggtcgct tgctgactcc taatgagatt
    14461 ggtcaaagaa acgacgtgat tgatgttttg taccatttac atcattcaaa ttctcttaaa
    14521 accatgctga cccgtatcgg tggtcgtgtc atgacgccgg agaagatgct gcaaaaatat
    14581 gaaaaagaat taccggaaga actccagaaa aatcaatttc tgcaaattgt ccatcgtttt
    14641 cttcgtgagc agatcccgtc aatagataag gaaaacttat cagtagtaca cggagatgta
    14701 aaccatcgta attggttagt atgtaaaaat tatttatatc tagtcgattg ggattcaatc
    14761 atgtttgcag accctgcggt ggatattgca acgattttag gaaattatgt gccactgtct
    14821 agctggaatc agtggctgat cagttatggc atacgcccga ctgacgaaat gcttgaaaaa
    14881 tttcattggt atgcggccat gaatttgctc caagaaatca ccagatattg caaacgtgaa
    14941 gatcatcgac ggatgaacga agaaatttta caattaaaac gaattttcag tggctgaggt
    15001 ttggcccaaa ctctgcggag atttggccag acctttttaa ttagaggaaa ggaatggaga
    15061 taaataatgc gtgtccgaaa tcgtccggga gcagcggaaa tgttagcggc tcacccgaat
    15121 tttgtaatta gtgatccaac attgtggaag ggaaaatgga atgaattgtt tgaaaacgat
    15181 catccaatcc acattgagat tggtatgggg aaaggccagt tcatcactgg aatggcaaaa
    15241 gcccatcctg aaatcaatta tattggagta gaaatgcagg tcagtgttgt ttcgattgca
    15301 ctagacaagt tgatcgaaca accgcttcct aacctgaaac ttctacatgt ggatggatca
    15361 gcgttgacag agtatttcgc tgacagcgaa gtagatcaaa tctatctgaa tttttccgat
    15421 ccatggccaa aaaaacgtca tgaaaaacgg cgattgacat ataagacttt tcttgctgta
    15481 gatgaacaga ttcttcgtcc aaatggagaa atccatttca aaacagataa tcaaggattg
    15541 tttgagtatt ctctggcaag tttttctcaa tatggcatga tcctcaaaca ggtatggtta
    15601 gatttacatc aaagtcaatt tgaaggcaat attatgacgg agtatgaaga aaaattttct
    15661 tcaaaaggtc aacgtattta tcgagtagaa gctcaatttc aagataagac caaaaagaaa
    15721 agtctgtgaa actaactttc tgctttcata aaaacattaa tatccataaa taaatctcag
    15781 aaaatgaaca ttacagatgt aatgtatttt ctgggattta tttgatttaa aatgtattta
    15841 tttcatcggt ttttccacaa ggtggaatca attggaagcg agaaaatgaa aataatcctt
    15901 tgtcggtgat cttcaattcg ggtatgacag ataatgttaa gaaagaaagt gtcaaaaaag
    15961 gatcaaaggt tttagctgct cctagttgat gtgctttagt tattaattca ttcaactgtt
    16021 catagacgat cgaaaaaggt tcctgtgtca tgattccgcc tataggaaga gaaacacaag
    16081 ctatttcctg ctgtttgttg acaacggtca tcccgccacc tttttgaatc aaaagattag
    16141 cagcatacag catatcctcg tcgtttgtcc caacgacaac tagattatgc gagtcatggg
    16201 caatcgttgt cgctatcgct ccttgtttca gctgaaatcc tttcactatg cctaatccaa
    16261 tgtttcccgt ttgatggtga cgctcgatca ccgcaatctt taattggtct ttttcgattg
    16321 aaggaatgaa tcgttcgcct tgtcggtcca ctttttctac caaatcttta gtcactagac
    16381 tattcgggat aatttcgatc acatgggctt gtgaagaaga aagaagcagc tctaacgaat
    16441 gtggatttaa tggttgagcg tttagttttg gcagacaaca cgtatatgaa tttgaatgat
    16501 cagcaaataa atggtcttgg agttgcccag attgaacaac taatgttccc ttatagaaaa
    16561 cttcatgaat tggcagcttg gtaagatcgt ctgttaagaa gaaatcagct tgatacccag
    16621 gggcaatcgc acctagtgtt ttcaaatcaa aacattctgc tgcattcaat gttgccatct
    16681 gaatggcttg taatggatcg attccaagtg caatagcttg acggacgata tgatcgattg
    16741 atccttcttt tactaaatca ttgatcagtt tatcatcggt gacaaagaag catcttcttg
    16801 aattttcagg tgtgatcgcc ggtagtagcg atgccagatc ttttgcaacg gtcccttctc
    16861 gaaccattaa atacatacca agatccaaac gttctttagc ttcttttgca gttgtagctt
    16921 catgatccgt ccgaatacct gctgcaggat aaacatttaa ctcttccatg ccgataccag
    16981 ctgcatgccc gtcaattttt ttcttatgct ggtgcatcaa ttggatcttt tctaggatgt
    17041 ctgtttcatt atttgctact gcttgatagt tcatgacttc agctaagcct aaaacttcgg
    17101 gtttttgtaa aaaaggggct aatttttttg catttaaatt tgcaccattg gtctcaaaag
    17161 gggttacagg tacacaagaa ggtagcataa aaaagatatt catgagtgtt tgtcttgcat
    17221 cttctatcat aaactcaaga cctgtactcc cagcaatgtt tcctatttca tggggatctg
    17281 tcactacagt agtaactcca tgaagcaagg atacttttga aaattcgctt ggtgataaaa
    17341 gtgagctttc gatatgcaca tggccatcga tgaatcctgg cactagatat tgcccattag
    17401 cattatgtac gctttttcct tcgtattcac cgattcctac gatcattcct tcgcagattg
    17461 ctacatcttt ttccatgatt tctttcgtaa agacgttgac gatcttagca tgcttgatca
    17521 ctaggtcagc aggctttttt ttacttgcta cttcgatata tttccttaat gttgtttcca
    17581 atggagtgcc tcccaagata aatttttata agtataacat aaattcggct tttgccaata
    17641 tttttttgta aattcatttt atttgaaaca aaaagatcaa aaagacgtat gttttttgtg
    17701 gaggggagaa taatggaaat aaaacgatta tcggatcaaa tgataagtga aagttttgcc
    17761 aaacatgccc aagacgtata tatattgcca gcttcagaag tattttacat ctattttcgc
    17821 tatggaaaaa aacgaacatt ctggaagaaa gtctcttcgg cagatggaca gcaactgatc
    17881 tcaagattta agtatttggg acagatggat atcggagaaa aaagaaaagc tcagttaggt
    17941 gcatttactt attctttaga aaaacaggaa gttcgtctgc ggttgtcttc tgtaggagat
    18001 taccttcaac gagagagttt agtgattcgg ctattatacg gtacttttga aacaccccat
    18061 cgttgcttta gttctcgaga ttttgcaact attttacaga aaaccgtcag acgtggatta
    18121 taccttttca gcgggacagt cggatcaggc aagacttctc taatgtatcg gttggctttg
    18181 gaagaaaaat tgcaagtgat tactatcgaa gatcctgtag agatagaaga accgtttttt
    18241 ctgcagctac agatcaatcc gaaaatagca cagacttatg atacactttt gaaactcgca
    18301 ttacggcatc gtccagactt gctaatcatt ggcgagataa gagatgaatc aactgcacat
    18361 gctgcggtaa gagcagcact aacagggcac cgggtttttg caaccgtaca tgcacgcaat
    18421 cttgaaggta cattggtcag aatccatgaa ctgacaaaga aaaaagaaga gctatctgaa
    18481 tgtttagctg gtgtcattta tcaagaactt ttggaagatg ccaatcacca tccttctgtt
    18541 ttatggagtt atcaatttat ggaggaagaa tcagtaaaaa aatcatggga ggaaagttat
    18601 gaagaagctc aggaaaatat ttggcagtca gaactattct aaaggattaa caagaagcca
    18661 gcaaaaccag tttgcacaaa ccatgggaga actgctttta tctggtttta gtctgcaaga
    18721 atccctttat ttattactca cgatccgttc gtttccacct gttattttgg caaatatcca
    18781 acatcagcta aaggaagggc aagcgtttag tgaggtactt tcgcaaatag gatatggagc
    18841 agagcaactg gtacagatcg agttagctga acgtcatggg aatctgccga caactttgtt
    18901 ggaaattgcc aaacagtttc ggcttgttga aggatttcgc aatgatttga aaaagctgct
    18961 tgcatatccg atgctattgc tcaccttttt gctaggtatc ttgtttagta tgcggttgtt
    19021 cgttttgcct cagcttattg gttcaggaat ggtcatgcaa gagcattggg gtattcagct
    19081 gatacaagtc tttcattggt atgttctact attcatccta gcggttactc tattggcttt
    19141 actagtaagg tatcgtctga tgaagttgtc tcctgttgat cagagtgaat ggataagcag
    19201 acaattattg attggaccga tatattcctt gtatcaatcg gcttatttgt cactagagat
    19261 tggaaagctt tttcatgaag gtttcgaatt aaggcaaatc atagtctgct taaaggaaac
    19321 gaaacaagaa agtctgattc agcatcttgc ctctaagttg attcaaggat tggaggcagg
    19381 tatgtcattg gctgagcagt tccagaatta tcgttttcta tcagaagaat tcagtaaaat
    19441 tgttttacag ggagaggcaa agggaagttt aggtaaagaa ctgatttttt acagcgaatg
    19501 gacaagaaaa cagcttttca aacgtcttca tcactggatg cattttattc agccaatcat
    19561 ttttttgggc gtagcggctt tgattttatt agtttatgcc gctgttttat tacctgttta
    19621 cggaagcata gaggaggttt tgccatgaaa aagagaaaaa tatcttatta tagcggtttt
    19681 acattgatcg aaatgctaat cgtcttactg atcatcagtg tattagtgtt gttgttcgtc
    19741 cctaatttgt cgcgttatag gaatcatgta gaccaagaaa gtcgtgaagc gatcatccag
    19801 ttggtagaca cacaaaaaga gctctatgcg cttcaaaata atggaagagt tcctactgtg
    19861 gaggaattgt tgaatgaagg ctatatcaaa cgagaacatg cagaaatcta tcagcggcct
    19921 tgaaggtaag gttttagcat caggatatac gttgtttgaa tcactcttac tgattttatt
    19981 acttacaacg atcctttctt ttccagttct cgcattttct gcatggaaga atgaattggc
    20041 agtctatcaa tttttcggcc aatttgaaaa acgaatctat tctacgcaaa aaatcgctat
    20101 tgtgaatcag atccaaacag gcttttattg gaatagtgaa gagaatcaaa tcatttttca
    20161 tgttccgaat ccagataaaa taaattggga agtattagat attcctgatg agattacctt
    20221 gaaacgccat gcttctatta cttttgctgc tagaaccggg aatgaaagtt cactaaaagc
    20281 ttatcaattt tattgggaag aaaaagagcg gacgattacg tatcaattcc agatagggag
    20341 tggtcgctat acaaaaagaa ttgaataaaa caggatttgt tttgttggag tcccttacag
    20401 cgcttatgat cgcttctgtc tgtgtctact tagtgagctt tggccagctg tttttgcttc
    20461 aggcagaaaa gaaaagtggg gaggaactgc tatttattcg aatgatttat gaagatatca
    20521 agacgcagcg gatgtatcac aaaagaccag agcgaaggga agaaatgatc ccatacgata
    20581 tccaaattgt cgataataat ggagtaatcg aaaaagtgtt tgtcaatatg gagaaagaag
    20641 cgatcagtat tgaaaaaaca agtaattcca gcatttacgc tgattgaatg tctaattgga
    20701 ctgatgatat tgagcagtct attgctcaca ttcaatcttt tgattcagca aaccatccaa
    20761 gtaagtgaat ttttgcagcg taaagatcaa aaagagtggc taatattttt agcacaatta
    20821 gaagaagaac tcaagagcag tcacaatgtc tctgtgaaaa atcagcaact gcactacacg
    20881 atcgaggata aacagtatat catcgaaaga tatcaggcaa tgatacgtaa aagaagaact
    20941 tccggcggac atcaacctat gttaacaagt atcactgagc ttcaattatt ggaaaaagaa
    21001 cacacaattt ctttttatgt acactttgaa aatggagaag atggatatgc catatggaca
    21061 aaaaacgatc agtagaaaat gttataaggg ctctatttta ttttctgtct tgcttttatt
    21121 tcttttatgt acgtttttat tgatggcagc attagaaagc taccgtttgt caatggatct
    21181 taatatacga atcaaaaatt attatatagc aaaaataatg aagcagatgg ctttatcgga
    21241 acagtctacg cttgccgaaa gttcggaagg agttttttac tacacaactg gatctgtaac
    21301 atatcagtgg gtacaacagt ccctattctt agaggtggaa gtctcaccgt ttatctttcg
    21361 gttcacagaa gaagtaaaaa atgatacaga tactagtaca gaatgatgga atgttcgtga
    21421 aaacgccctc ttttctgtta taacactgtt tttaataaaa aaatgtttgc cgtttcacaa
    21481 ataaaaaaca gtgaaaacga ttgaaatttt gtcaattgtt cgatagaatt aaacaggtga
    21541 aaaaattgtt ataatatatc tagaaatgag gtgcaccatg ttaccagaaa aaatggaaca
    21601 agggtttaat caaaacctgg aagctattca gcttttacag aatgcattgg gaacttcctt
    21661 tttagaagcc tatgtcgaaa atgcagaaaa tttaatagat gattatcaag tacgtgtggt
    21721 ggatggagtg ccgacaaaag agacaacgca acggatcaca gctctttatg aagagttgaa
    21781 gaaactttct ttcgagccag aagaatggcg cagactgtct caattacttt tattgaaggg
    21841 cagccagaca gaacatttgc aggcgaacca tcaactcact cctgatagta tcggtttttt
    21901 atttgttttt cttattgaac aactatacac aaataaaaaa gcaccggtta agattctgga
    21961 tattgcggca ggaatgggca acttgttatt aacagttctt ttaaacctat cgaatgcggg
    22021 gtaccaaaca gaaggaattg gtgtggatat tgatgataca ttgttggctg tcgcagcttc
    22081 tacaagtgat ttgacacaag caaatgttca atactttcat caagacgggt tgcaggaatt
    22141 attaatagat cctgtcgatt ttgcaatcag cgatttgcca atcggttatt atccgaatga
    22201 cgaaaaagca aaagagtttt taacaagtac cgaagaaggc catagttatg cacatcattt
    22261 attgctagag caatcaatga aatatgtgaa gccggatggt tttggtttgt ttttgatgcc
    22321 gagtggattt ttagaaacgg atcaaagtga agaaatcaaa aaatggttta aagaagaagg
    22381 ttatcttcaa ggaatgattc aattgccaga tgaattgttc cgtaataaac aatcgcaaaa
    22441 aagtattttg atcttacaga aaaaaggacc acaagcacaa caagtaaaag aagtcttgct
    22501 tgtgaaactt gcttcattaa aagaaccaga gaaggtgacg gaatttttca acgagtttaa
    22561 aaactggaaa tcatctgctc tgtaatcatt caacgagaag gagagtcata tgtctaaaac
    22621 aattgcaatc aatgctggga gttcaagttt gaaatggcaa ttatatcaaa tgccagaaga
    22681 aaccgtagtt gcgaaaggga tcgtcgaacg tatcggttta caagattcta tttttaccat
    22741 caagtacggc gataatcaaa aattcgaaca aatcatggat atcgaaaacc acgaagtagc
    22801 agtaaaaatg ctgttagatc agttgattga actgcaaatt ttaagctctt atgatgaaat
    22861 caccggagta gggcaccgtg ttgttcacgg aggagaaact tatggggatt cagtagtgat
    22921 cgatgagaaa gtaatggacc gcattgctga attagcagaa tttgctcctt tgcataatcc
    22981 tgcaaactta atggggatca aagcgtttaa aaagatttta cctgacattt tgagtgtggc
    23041 tgtattcgat acatctttcc actcaacaat gccaaagcaa aattatttat atagcttacc
    23101 ggtgtcttat tatgaagatt tcaaagtaag aaaatatggc ttccatggca caagtcatcg
    23161 ttatgtgtcg gaacgtgcgg caaaattatt gaacaaaccg atcgaagaac taaaaatcat
    23221 cacttgccac ttaggaaatg gtgtatcgat cacagcagtc gatggaggta aatccatgga
    23281 tacttctatg ggattcacac cattagctgg ggtgacaatg ggtacacgtt caggtgatat
    23341 cgatccagct gttctcccat atttaatgga aaaattagag attgatattg atgaaatgat
    23401 caatgttttg aacaagaaat ctggtctgct aggcctaaca ggattgtcta gtgatatgcg
    23461 tgatcttgaa aagaactatg aaaaagaaca tattcgttta gcttatgaca ttttcgtgga
    23521 ccgcatccga aaatacatcg gcggttatgt gacaacgatg aatggtgttg atgcaattgt
    23581 ctttactgct ggtatcggcg agaatgacgg acatgtacgt agtgaaatca tcaaaggtat
    23641 gtcttggttt ggttgtgaaa tcgatccagc gttgaatgaa ttgcgtggtg aagaagtaga
    23701 tatctctaca aaagaatcaa aagtaagagt attagtgatc ccaacagatg aggaactaat
    23761 gattgcccgt gatgtggagc gtctgcgcgg ctaagccaaa gcattcgtta ggataaaaga
    23821 tgaaacgcat gcaagaggga attcttgcat gtgttttttt tgcggagaag accgcaaaat
    23881 ggtaatttaa cttcaatttc ccttcttttt tacaggatct tcttagggaa ttttgcttgg
    23941 aaaagtggtc aaaagggaaa agactggcat tttatttatt gattttcagg aaaatcccaa
    24001 tattttattt tcattcatct ttataaggga cattacatat gtaaaaagaa aaaacatttt
    24061 tagatcagcc tcgtgggctg tcgactttat tcttcgcttt atgtttagct agaaaaccga
    24121 tccgaaacat tcattgatcg aataataaaa acttgatttt ccacctgtac tttgcctgaa
    24181 tcgtgaaagc acaggtatga aaatcaagtt ttttgttgcg aaaaaacgtc ctagtcaatc
    24241 agttggccga gcggctggcg gctgatctgc tcctgatcaa taacgagtgc ttcttggaag
    24301 tgttgctgca aaaagtagcg gtcatggctg actgctagga gcgttccttg atagtcttgc
    24361 agcatttctt caatttcttc tcgagcatag ctgtcgaggt gattggttgg ttcatccaaa
    24421 atcaggacgt tgattttttc taaaaagagt ttcagcaacg ccaaacggat tttttcacca
    24481 ccggataaat ctttgatccg tttacccaca tcgctttgat aaaagccact tttggctaat
    24541 tgccgtcgag cgacttcttc gtttccagtc aatgtgatgg cgtaagccag caaacggcta
    24601 tcgggatcgg caaactgcag ctgctgcggc aaataaccga tttgcaagct actacctaat
    24661 ttgatggtgc caccgtaagg cttttcctca ccgagtaata atttgagtaa ggttgatttt
    24721 cctgcaccat tgtcgccgat gatggctaaa tgatctccac gatagactgc aaaactgctg
    24781 tctttgaata gtacttgatc agcgtactgc tggtgcaagt tttgaatgat gaaaacttct
    24841 ttgccagatt ttccaccatt agacaagctt tgcagccgat ttttcggggg aggtggcggt
    24901 ttgacgagtg tcagtttcgc caggcggcgt tccagttctt tggctttttt gaagaaggac
    24961 tcattgtctc cttcatgtgc ccactgacga aaacgacgaa tcatgacttt caaccgctgg
    25021 acttcttttt gctgcagttc gtaatttttt gtcagctcag ccaagcgagc ttctttcaag
    25081 actcgaaaac ggctgtagtt tccgggatac tcaatcaatg caccttcttc cagttcccat
    25141 gtttttgtgg tgacttgatc gagaaattgc cgatcatgag aaacgaccag aaccgatttt
    25201 tttgtattct gtaaatagtt ttccaaccag cgaatactgt taagatctaa atgattggtc
    25261 ggttcatcga gtaaaagtaa atcggcatcg gctgctaaaa ttttggctaa agcgacttta
    25321 actcgttcgc ctccgctaag ttctgttaac cgtgcttcct gacagtgtgg aatccctaag
    25381 ccttgcaaga tcgctgcaat ccggtcttct aatacgtaac cgcctagttc ttcaaagcga
    25441 tgctgcagct caccgtaacg ggccagacac ttctctaagt ttgtatctgg ttctgtcatc
    25501 aatgcttcgt agcgactcat ttgctgtttg aggtcctgta tctcagcggc agaatggagc
    25561 aagtagtggt tgacggtcgc ttcggaaact gtaagttctt gttcgaccgt cccgatcttt
    25621 aagccttttt tgcgactgat aacaccgctg tcgacacctt ctctgccggt caagatttgt
    25681 aaaaaggttg atttgccagt tccgttgata tcgactaagg caattttttc accagcttca
    25741 atttgacagg ataaatcctc aaataatgga ttcccgccga ttgttttttt aatagaaatt
    25801 gcttgtatga tcatgatctc tctccttgag accactcaaa aaggccatgg ttatccctgg
    25861 cctgtgttat ttaaactcag attatcatga gaaaaatacc ttgagatggc cattgatttt
    25921 acgtgaattc aactgttttg gacaaacttg tcccgtcaac agcaactgta aaaccaattg
    25981 catggctaaa tgcgatatgg tatcgttggt aattttcatt acttgttatt ttagtcatgc
    26041 gcatcatagt attttccctc cttcgtgtag ttgtcttttc cagtgtacct gaaagtgtca
    26101 gaaagttcaa ctagcgctga catttactta atatttatcg atgtgtaatt ctgtcagaga
    26161 gcttttttta tctgactgag ggtaatgtct cgtggaccac atcattgtat tctgagctaa
    26221 accagcgttc aggcgaaatc atatggtgtt tgataaaaaa ctttgtctac atgacttcct
    26281 tttgccattt gcttaatacc gttgcattaa acagctcttc tctttggaaa tctcaccaac
    26341 atcgcgttca ggagtatcgt tgtggttgca atggcaagcg gtagcctttc atcaattctt
    26401 gaatgattta ccgaatgtag cacttgcctt ctcgcggtac ccgaacccga aataaaattt
    26461 tagtttggcg ataaaatcgg tatccgccat cgatacttca tattttttcg tatcagattc
    26521 atcggaaact tccacattga cgaaccaata agctttggca tgcttaggtc gttttaaacc
    26581 acaccagggc gactaatcgc cctggtgttc acttttttta ttttacttga actcattgac
    26641 tgttttccct ttattttaaa cattataaag accgatgagt gcttgccgcg tcttttgttt
    26701 ttatcacggt tttttgtgca aaaaaacctg ccgatagttg gcaggtcgtc tacgtgttaa
    26761 gctttggtgt ggtctttagc tggcaattgg ttttctaaat cagttcggac aacaagcaca
    26821 tcgcaagctg cgtttctaat aacatattca gaaacagaac caataaataa acgttctaca
    26881 gcattcaatc ctgtggctcc taacataatc aaatctactt gattatcttc tgggatttct
    26941 ctggcaataa tttgttttgg tgagccatat tctacgacag aggttacatt attcaagcca
    27001 gctttcttcg cattgctttc ataatctgcc aatgtttgct ttgccatttc agtagcttgt
    27061 tctgcaagca ttccatcaaa ggatgaaacg gtttggaaag aacgtgtgtc aatgacatgt
    27121 gctaatagaa gttctccgtt atttcggata gctacattga ccgccttttt aaaagctaat
    27181 tcagcttcat cagaaccatc tacggcaacc atgatttttt tgtattgttg taacataagc
    27241 attcactcct caagtcaatc gtattgtact cttttattgt agaacacttt agggaaaaag
    27301 agaattattt actgctttgg ttcagcaatt ttttttgcaa atcggatact gaaggtggca
    27361 gaaacaataa ttacgatcag taaaaagacc aaggcaataa agcgttgatt gaaaaaagct
    27421 gttaaaatac ccattacgcc taaaacagca tatgcagcac cgtaaaattg taggaatcct
    27481 tgatttttat catttttgtt gatcaataca aaaaagatat cctgtttatt aaataaataa
    27541 tagctaacga ataataatac taaagtggcg ataatcatta aaatcctaat catgaacaaa
    27601 tcctccaaaa taagcaacaa atagaaaaag caatttgcca agagcaaatt gctttattct
    27661 ttaaaagatg gtaatctccg ttaatgccgt tgtttgtcct gtagtattga tcccgcgaat
    27721 tcaagcaggt gggtcccatg gtcgtagctt cgaactacca aatgataagg catgttacca
    27781 gcagtgacac aagacgggtc ccaagatatc aataaaaatg ttcggtcaac acacaaagag
    27841 gacaattcga acatcacaga tttcccacct ctatagtagc atatgaattt aaaaagagca
    27901 atgagagagc tcgcagaaac agaaccgttt tcttgtctag ttttttggag gatgcttttt
    27961 ccactctttt atacggtaat actgttgacc cagagcctgt tcatatttcc ctgtatcttt
    28021 tggctgataa tactgagcat tcttcagttt atctggtaaa tattgctgat tgacccaagc
    28081 ctgatcaaaa tgatgaggat actgataacc cacgccacgg ttgagtgatt tcgcaccttt
    28141 gtaatggcta tctctcaaat gatcaggaac atctccagct ttgccctctc gaatatcagc
    28201 aagtgctgca tctaatgcca tgtaggcaga attggatttt ggcgacaagc aaagatcgac
    28261 gacgacgtct gctagtggga tacgtgcctc aggcaatccc aatttttcag cagctaatac
    28321 agcgttcact gtacgagccg cagcagcagg attacctaaa cctatatctt cataaccgat
    28381 aaccatcaga cgccggcaaa tacttgccag atcacctgcc tcaactaaac gggcgagata
    28441 atgaagtgca gcatccacat cacttccacg aatcgatttt tgaaaagccg aaataacgtc
    28501 ataatgagca tctccatttt tgtcatgggt aagagctttt ctttggacac attcttcaat
    28561 gatcgacagt gtaagatgaa tccttccttc tttatctgaa ggagtcgata gtgtagccag
    28621 ttccagccca ttcaatgcac tccgcaaatc gccgttcgtt gcacgcgaaa ggtgaagaag
    28681 agcatcttcg tctaactgga ttgcttgttg tccaaggccg cgttctttat cccttaaagc
    28741 atgttcgact gccaactgga tgtcttgttc tgttaatggt ttgacttcaa agatctgtgt
    28801 acggctgcgg atcgctggat tgatggtaat ataaggattc tcagtagtcg ctccgatcaa
    28861 gatgattcgg ccactttcta aatgaggaag cagaaaatcc tgttttgttt tatctaatcg
    28921 gtgtacttca tccaataaga ggatcactgt accactcatt tttgcttctt ccgctacaac
    28981 ttgtagatcc ttttttgaat ctgtagcggc gtttagcatg cgaaatgcat aattagtaga
    29041 gccggcgatc gcacttgcga tactcgtttt tccagtacca ggaggtccat atagaatcat
    29101 ggaagagagc atgcgagcat cgaccatgcg gcgtatgatt tttccttccc cgactagatg
    29161 ctgctgacca acgacttcat cgatcgttcg aggtctcatg cgataagcca atggctgttg
    29221 cataggattt tcctccttct tgtttttacg tcccttaatt atatcatatt tagcaagatg
    29281 cctagaaaga acagccttct gttgttttgt tttcatagct tatccaacta atattgtgta
    29341 tgataaagta ggtgagaaaa atgacggata aacaaaagat agatgaattt ttttggaata
    29401 aaacgacgcc agaagtggca cgggatttat tggggatgta tttggagcat gacacaccag
    29461 aaggaagatt agctggatat attgtagacg cggaagcata cctaggtcca gaagacgagg
    29521 ccgcccatag ctatggttta agaagaacac cgcgtgtgcg ggcaatgtat gaaaaaccag
    29581 gtacgatcta cctttacacg atgcatactc atcggatttt gaacatcatt actcagccag
    29641 aagggattcc tcaaggagta atgatacgtg caatcgaacc cgccgcaatg attgatcaaa
    29701 tgagtaaaaa tcgtggcgga aaaactggtc cagatatcag caacggtccg ggaaaattag
    29761 tggaagcatt agcaattcct caagaacttt atggtcagtc tatagcagat agttcgttac
    29821 gcttagtttt tgagaaaaaa aagacaccaa aaaagatcaa tgctttgcct agaatcggta
    29881 tcccaaacaa aggtgtatgg acagaaaagc cgttgcgttt tgttgtgtcg ggaaatcctt
    29941 atatctcttt acaaagaaaa aaccaaatcg agaaagattg gggttggagg aaagaaaatg
    30001 aaaaagaagg atctattaac atatttagat gaaataattg aaaaacaagt gacagactat
    30061 gatgtagctt tagattggga tacaaaaaat catacgatcg agatcgttgt tcgcttgttt
    30121 gcagaaaata aagcacaatt tgagattgat gatgcagaag ggatcgtttc gcaagaacca
    30181 attatcgaat ttgaagacgg cgtacttctt tttaatcctc agaaatctgt ttttgatgaa
    30241 cgcaattatc tagcagtaat cccttatgaa ggaaaaaaag ggttggcaaa atcagtagct
    30301 gattctttag tggaatatct aaatgaagta ttagctcaag ggcaaagcga tttgcttgat
    30361 tttttggatg aagacgatga cgaagctgta ttcgagcttc actgggataa tcaacaacta
    30421 gcagaattag tagaagtaaa acagcaaaat gaagcagata cttatttacc gtacccaagt
    30481 tattaaggag tgacagatat gaaatggaat gaagtaaaag tagaaactga aagtgaagca
    30541 gtcgaagcag ttgctaatat tttgatggag gcaggggcga gtggtgtcgc gattgaagat
    30601 tctttagatg ttgaacattt tcagtcggat caatatggtg agattttaga taaaaatgat
    30661 tttacatcgc tgaaagaagg cgcactagtc atggcttatt ttccggaaac gactttctta
    30721 ccggaaatct tgccttttat caaagatcgt atcacacaat tacctgagtt tggtctagct
    30781 attggaaaaa ataaagtaac agttagtgaa gtagaggaaa gcaactgggc aacagcctgg
    30841 aagaaatact atcatccggt gcgtattacg cgaatgctga cggttgcgcc aagctgggaa
    30901 aactaccaga cagaagatcc tctagaaaaa attatcacac tagatcctgg tatggcattt
    30961 ggcacaggca ctcatcccac gacttccttg acattgacag cactagaaac agtcttgcgt
    31021 ggtggtgaaa cattgctaga tgtgggaact gggtcgggcg tgctaagtat tgcagcaaaa
    31081 tatcttggtg caaaagatgt ctatgccttt gatttagacg aagtagccgt tcgttcggcg
    31141 aaagaaaata tggatatgaa tgaaatagcc aaagatgtcc atgtatcagc taacgatctt
    31201 ttgacaggaa tcgagattga aagtgatgta atcgttgcga acattttagc agatatcatc
    31261 ttattgatga tcccagatgc ttggcgttta ctgaagcaaa caggcacgct gatcgtttca
    31321 ggaatcattg aagagaagaa gcaaatggtt ctggatgcca tgttagaaca agggtttatc
    31381 gtggatcaaa ttttccagca aaaagactgg tatgcaatta ttttgaaaaa accagaggtg
    31441 gaatagatgc agcggtattt tttaaaagaa tcatatgaat caaaagacta ttttgtttta
    31501 gaaggggaaa actatcacca cattgtacgg gttatgagga tggaacccga ggatgaagtt
    31561 tttttggcat tcaatgatca aatagccatt gttgcagaaa tcacggagat tactgaagag
    31621 gctgtttatc tgaaagaaat taaaaaagaa gaaaatcaaa aggaactgcc aagttcgatt
    31681 accatagcga gtggctatcc taaaggggaa aagctggatt ggatcgttca gaaatccact
    31741 gaattaggag cgcaagcttt tcttggtttc ccggcatcag cctctgtcgt taaatgggat
    31801 gcaaaaaaac gtatgaaacg acaacaacgg cttgaaaaga ttgctcaaga ggcagcggaa
    31861 caatcccatc ggcagatcgt gccatcagta gagttattgg aaacgaaaca agatttattt
    31921 gagcggctag atgattatga cgtggtcttg gttgcttatg aggaatcagc aaagcaaggc
    31981 gaaaaatcaa atgcagccaa aattcttagt gaaacaaaac cagatagtcg aatcttagct
    32041 gtctttggac cagaaggtgg attatcccgc gatgaagtcg attattttgt agaacacaaa
    32101 gggatacttt gtggattagg accaagaatc cttcgtacag aaacagcacc tctttattta
    32161 ttaagtgctg ctagctatca atgggaatta atgaatcctt ctttcagcta acgttcttca
    32221 ttaataaaag agtatcgaca gaaagaattg aaaaaatagt caacaatttg tataatagaa
    32281 acaattattt atacaatgga tacgaaaaat cgtttgaaaa agcttttaaa aagcgaacat
    32341 ttccgtaaaa aaacgtataa ctgcacggat tttgggacaa ttatcaaaca gatatttgtc
    32401 ccgattcccg ttttaccgaa aaaagaaagg gggatcgatt cctgtgacaa aagaagaaat
    32461 tttaacggga cccggtgtca taaagctcgt tagtcaatac atgggtcccg aacatgtagc
    32521 atttgttgag aaagcatgcg aatacgcgac tgctgcacat gacggacagt ttagaaaatc
    32581 aggcgaaccc tacattattc atcctatcca agtcgcaggt atattagcag atttaaaaat
    32641 ggatccccat acagtggcta caggcttctt acatgatgtt gttgaagata cagaaatcac
    32701 tttagaagat ctgagagaag aatttggcga tgacgttgct atgttagtgg acggcgtaac
    32761 caaattaggg aaaataaaat ataaatccca cgaagagcag ctagcagaaa accaccgaaa
    32821 gatgctgcta gcaatggctc aagatttacg agttatcatg gtcaaattag ctgatagact
    32881 acataacatg cgtacgttga agcacctgcg agaagataag caaagaagga tcgctcagga
    32941 aactttagaa atctatgcgc ctcttgcaca tcgtctaggg atcagccgga tcaaatggga
    33001 attagaagat acagcacttc gttatctaaa tccaaaacag tattaccgta tcgtccactt
    33061 gatgcagacg aagagagaag aacgtgaaaa atacgtaagt ggtactgttg aagatattcg
    33121 aatagcgaca gaagagctgg ggatttttgc agaaatctat ggacggccaa aacacattta
    33181 ttcaatctat cgtaaaatga aagatcagaa aaagcagttc aacgaaattt atgacttgct
    33241 agctattcga gtgatcgtag attcaatcaa ggattgttat gctgtattag gagcaatcca
    33301 cacaaaatgg aaaccaatgc ccggcagatt taaggattat attgccatgc caaaagctaa
    33361 tatgtaccaa tctttgcata ctaccgtgat cggtccggca ggaaatccgg tagaaattca
    33421 aatccgaaca caggaaatgc atgaaatcgc tgaattcggg gttgctgcac actgggccta
    33481 taaagaagga aaaaatgaaa aagtagaacc agatggtatg acgaaacaat taagctggtt
    33541 ccatgagata ctcgaacttc aagacgaaag ctatgatgct tctgaattta tggaaggcgt
    33601 aaaaggagat atctttagtg ataaagtcta cgtcttcaca ccaaaaggag acgttactga
    33661 gttaccaaaa ggatccggac cattggactt tgcatacagt atccatacag atatcggtaa
    33721 caaaaccact ggtgcaaaag taaatggcaa aatggtgcag cttgattaca aattgaaaaa
    33781 cggagatatc attgagatca tgacttctcc aaattcattt ggcccaagtc gcgactggtt
    33841 gaaattagtt gctactagca aagcaagaaa taagatcaaa cgtttcttca aagcccaaga
    33901 tcgagaagaa aatgtgatca aaggccacga atccgtggtc aaatgtatta cagatctagg
    33961 atttacgcct aaagatattt tgacgaagaa caaactgcaa gaagcactcg atcgttttaa
    34021 ttatcaaaca gaagatgatc tctatgcagc tgtagggtat ggagaagtta gccccttgac
    34081 gatggccaat cgtctgactg aaaaagaacg taaagaacaa aaaatcgagc agcaaaagca
    34141 agaagcagaa gaaatcatga atcagccgaa aaaagaacct gacaaaatga aagtacgtca
    34201 tgaaggtggc gttgtcattc aaggggtaga aaacttacta attcgtatca gtcgctgctg
    34261 taatccgatt cctggtgatg atatcgttgg ttatatcact aaaggcagag ggatatccat
    34321 tcatcgtcga gattgtccga atgttcagcc tgacaaacca aatgtagcag aacgtttgat
    34381 tgaagtcgaa tgggaagata catcgaatac acgaaaagag tatgatgcag atttggaaat
    34441 ttacggctat aatcgttcag gcttattgaa tgatgtactt caaacagtca atgcgctaac
    34501 gaaaaatctc aacagcgttg aagcacggac gaataaagat aaaatggcga cgatccattt
    34561 gacggttggt atccagaatt tatcccacct aaagagtatc gtggataaaa tcaaagcagt
    34621 acctgatgtc tacagtgtac gccggacgaa tggataggag gacagatatg agagcagttg
    34681 ttcaacgggt aagtaaagct gctgtgtcag tcgatcagca agaaattgga aaaattggct
    34741 acggcctact tgttttatta ggcattcatg aaaccgatac gcaaaaagat gtggactatc
    34801 tggtaaaaaa aattgcacaa atgcgtattt ttgaagatga acaaggaaaa atgaatatta
    34861 gtgtagaaga tgtgggagga caaattttaa gtatctctca attcacactg tttgcggata
    34921 cgaaaaaagg aaatcgacca agctttgtgg aagccgcacg accagaaaca gctattcctt
    34981 tatacgaatc attcaatgaa ggaatcagaa gtcgcggcat cacagtcgct accggacaat
    35041 tcggagcaga tatgtcggtt tctctcgtca atgatggacc tgtaacgatc attattgatt
    35101 ctaaaaacaa ataaagtaat ctggttttca ctagtaaaaa ctatgaaaca cgtcgcttaa
    35161 gttagactga taaactctaa cttaagtggc ttttttgtct gcaaaaagcg cttcttttgt
    35221 cagcagtcgg aaaaaaagtt aagcttaaat tatacaagtt attttttata ggaggttgct
    35281 gaagatgaaa gaagtaccag tagtagatat caaaatcaca gatttaaaaa aattgtatca
    35341 aaaaggatac aacatgttgg aggagttacg ccatgaagcg gacgctcccg tcgtcaaagc
    35401 aaagattttc aataaagaag ctatcacgat ctacggatca tcagcagcaa aagtatttta
    35461 tgatccaagg aatttcaaac gaaaaggtgc tatgccaaaa ttagtattga agacactttt
    35521 tggtcaaggt ggagtacaga ctttggatgg agctgctcat caccatcgga aaaatatttt
    35581 tatggatcta atgacaccgg aaagaatgga agattatcat cgtatcttag ataagaatct
    35641 gacacaagca cttgaagcac agcatggtca atttgaacta tttgatttaa gtaagatggt
    35701 tttctttaca tctatttgcg agtgggcagg tatcaattta tcagcgattt ctaaagatga
    35761 ggtcgaaaaa ttagcagaat atcaaatctc tatgatcagc ggaacgttta cttctccgat
    35821 tgatcacata aaaggtgtcg agaatcgcaa aaaatcagaa aaatgggcgc aaggcttgat
    35881 tgaagaagct cgacaaaatc ctgtggctgg aaaagaaaat gtggcattat atgcttttgc
    35941 caatgccaca gatttagatg gtcagctttt acctctggaa gtggcagctg ttgaattatt
    36001 gaacatcatc cgtccaacgg ttgctctaac tgtgtgggcg gcattgatgg gacatgcact
    36061 tttcagtaga ccagatttgt atcagcaatt aaaaaatgac ttttcgacgt tacaagatcc
    36121 gtttattcaa gaaatgagac gctattatcc tttcttccca atgctacctg cgatttcgct
    36181 gaaagaagta gaagtagatg gttatcgtat accagaaggc agttgggtta ttttagattt
    36241 atatggaaca gaccatgatg aacgaacagt tgaagcgcca gattccttta tgatcaaacg
    36301 gtatgtaggg aaagcaaaag atatttccta taaagaagaa tatgagatga ttgcacaagg
    36361 cgggggaaat tttcgacaaa tgcatcgctg tgcaggagaa tggatcactc ttcatagtct
    36421 acgtgtgttt tccgatcagc tagtaaataa gtttgagttc tctgtacctg aacaagattg
    36481 gacgattcca ttcaatcagt ttccgactta tccgaatagc cgagcgctac tttacaagaa
    36541 ttagattagc ataaacttat cgaaaaaaag gaagatcaaa cagtgctgat cttccttttt
    36601 tcatattact ataaattgat aaggtcaatt gatcaaaatc cacgaaatgc ttcatcaatg
    36661 gtttgataat cttcattgga taaatgcaaa tctaaagctt ggacattgtc agatacttgc
    36721 tctggacgct tagctccagg tataactaca gacactcgtg gattcttcat gtaccaagct
    36781 aaaacaagtt gcgcaggagt ggcttggtat cttttagcaa tggggcgaag tacatcgaca
    36841 gcggttaaaa tttctctgaa acgttctcct tggaaatctg gatttctttt tcgaggatct
    36901 ccttcaccaa attgtttttc ctcaccaagt tcatattttc ctgtcagcaa accggaagca
    36961 agaggaaaat agggaacaaa actgattttg tttttttcaa gataagggaa taattctttt
    37021 tcagcctgcc gatggatcaa actgtattta tcttctacta catcgacata tccatctgca
    37081 ttcgcttctt tcaattgttc taacgtgaag tttgacacac cgactgctcg gatttttcct
    37141 gcttctttta attcgtgcaa tgtagcaact gattcatttt ttggtgtgct ttcatccgga
    37201 aagtggatat aaaaaatgtc gatgtaatca gtttgcagac gttttagtgc atcttccacc
    37261 gattgtttga gaaactcagg agaattatca aatgttcgcc ctttatttgg tacatgagca
    37321 gcttttgtag cgatgacgac acgtgagcga tcatattctt tttccttcag tacctcgcca
    37381 atcagttctt cggaacggcc gtttccgtaa gcataagcgg tatcgatcaa attgatcccg
    37441 ctattcaaag ctgtacgaac tacttgtttt cctgtctcat cttccaaacc gtcaaaaaga
    37501 ttatgtcctc caacggcatt tgctcctaat ccaagctgtt ctgcgtagac ttgtgaatgt
    37561 ccgattctta tttctgccat agctgattcc tcctttttgt ctagtctaaa acaaaatacc
    37621 aaaaagctga actaaaacgt ttttgtgagg ggaaatagtc agatatctct tgactttgag
    37681 actggttttt agtaggttat aagtaatatc gaatgtcaat gaaagagcga gtaagttttt
    37741 gaaactttac aagagaggat catcgaggct gaaaatgatc taaagtttca ttagcctaaa
    37801 gacactctgg agatggacga gtgaaaagcc gtcccgcgta cgagcgttat tcgtaagagg
    37861 aaagagaaat ctttcaaaat taggtggtac cgtgtatgaa tttcattcac ccttgttcat
    37921 gatgaacaag ggtgattttt ttatttacat agctaagaaa gggagagaaa actatgagtt
    37981 tccaaagacc aaaaggaaca aatgatattt tgccaggcga atcagaaaaa tggcagtttg
    38041 tcgaagaaac agcacgttta ttattcagag attatcagta taatgaaatg cgtacaccga
    38101 tttttgaaca ctatgaagtg atatcccgaa gtgtagggga tacaacggat atcgtttcaa
    38161 aagagatgta tgatttttat gacaaagggg aacgccatgt gacgcttcga ccagaaggta
    38221 cagcaccaat cgttcgttct tttgttgagc ataaattgtt cggcccagag tatgcgaaac
    38281 cgttcaaaac tttttatatg ggaccaatgt ttcgctacga acgccctcaa aaaggacggt
    38341 tacgtcaatt ccatcaaatc ggagtagaag catttggatc agaaaaccct gcaactgatg
    38401 tggagagcat ggtgatggca cttgatttct tcaaacagtt ggggatcacg caaatccggc
    38461 tagtcatcaa ttcattaggt gataaagaaa cgcgtcaagc ctatcgtcaa gcattgatcg
    38521 actatctttt accttttgaa tcagagctga gtgaagactc taaacgccgc cttcatgaaa
    38581 atccattacg cgtattggac agtaaagaca aacgagatca aaaatttgtg gcagaagctc
    38641 catctatctt agattatcta agcgagcctg ctcaaaaaca ttttgatacc gttgtttcca
    38701 tgctggatgc attggctgtt ccttatgaaa tcgacagtaa tatggttcgc ggactagatt
    38761 attacacaca tacgattttt gaaatcatga gtgacgcacc aaaaatgggg gcacaagcaa
    38821 cgatttgcgc aggcggacgt tatgacaatc tagtggaaga actaggtggt ccttcaacac
    38881 ctggcttcgg atttgcaatg ggtatcgagc gattactctt gactatggag gcagaggaag
    38941 tagttatccc tgcatttaat gagttggatg cttacgtggt cgcactcgga gacgaaacaa
    39001 atatcgaagc attgaaagtg gtccaagcga ttcgaaactt tggtttttca gctgaccgcg
    39061 acttcatgaa ccgtaaagca aaagcgcaat ttaagacagc agataaagcg aatgccaaat
    39121 tagtattggt gatcggtgaa gatgagcttg ctagtcaaac gattaatgtc aaagcaatgg
    39181 caagccgaaa agaaaaaagt tttccattag aagagatata tgaacatttt gatgaagtct
    39241 atgatgaaat gactttattc gagtaggagg aaaaaaataa atggcacaaa gaacgatcta
    39301 ttgcggatta gtttccgaac aacaattaga acaaacagtt acattaaaag gatgggtaca
    39361 aaaaagacga gaccttgggg gagtaatctt tatcgaccta cgtgatcgtg aaggaatcgt
    39421 tcaaatcgtc tttaatcctg aaatcaacaa agaagcatgg gaaatcgcgg ataaatgtcg
    39481 cagtgaatac gtgatcgaag tgactgggga agttagaaga cgcggagaaa atgcagtaaa
    39541 tcctaaaatg gccactggtg aattcgaagt gattgcaagt gaaatcgtga ttttgaatca
    39601 agcaaaaacc ccgccatttc aaattgaaga tgatcaagca atcagtgacg aaatacgttt
    39661 gaaatatcgt tatttggatc tgcgtcgtcc aaaaatgact aaaaacatca aattaagaaa
    39721 tgcgacaaca aaagcgattc gtcattattt agatgataat gactttctcg atatcgaaac
    39781 accttaccta gggaaatcta caccagaagg cgcaagagac tacctagttc cttcacgtgt
    39841 ccatgctgga catttctatg cgttgccgca atctcctcaa ttgtttaaac aattgctgat
    39901 gaatgctgga ttcgaccgtt attatcaaat cgttcgttgt ttccgcgatg aagatttacg
    39961 tggagaccgt cagcctgaat ttacgcaagt agatattgaa acaacgttct taacagcaga
    40021 agaaatccaa acttacacag agggattgat tgcgaaggta atgaaagaag ttcgcggtat
    40081 cgaagtcaca ttgccatttc ctcgtatgac ctatgatgaa gcaatggctc gctacggcag
    40141 cgacaaaccg gatacacgtt ttgatatgga attgatcgat ctaagcgata cagtcaaaga
    40201 agtagaattc aaagtcttcc aaatggctct tgaaaacggc ggtgtcgtga aagcattgaa
    40261 tgctaaaggc gctgcagatc gctactcacg aaaagacatg gatcagttag gtcaatatgt
    40321 aggacaattc ggagctaagg ggcttgcatg gctgaaagta gaagaagacg gattgaaagg
    40381 tccaattgct aaattcatgg gagaagcaac agaagcaatc attaaagcaa cagatgcaaa
    40441 accaggtgac ttgctgatgt ttggcgcaga taaaagtgaa atcgtcgctg ctgcattagg
    40501 tgctatccgg acacgtttag gaaaagaatt aggactaatt gatgaatcga aattcaattt
    40561 cttatgggta actgattggc cacaatttga attttcagaa gaagaaggtc gatatgtctc
    40621 tgctcaccat ccattcacga tgccaaaaga agaagatatc ccgttattgg ctgaagaccc
    40681 tgcaaaagtc tatgcagaag cttatgatat cgtcttgaac ggttatgaat taggcggagg
    40741 ttctatccgt attcatactc gtgagcttca agaaaaagta ctagaaactc taggctttac
    40801 aaaagaacaa atggaagaac aatttggttt cttgttaaca gctttagatt acggattccc
    40861 tccacatggc ggtattgcat taggattgga ccgttttgca atgttattag ccggagaaga
    40921 taatattcgt gaagtcattg cctttcctaa aaacggaaaa gcagcagatg tgatgagtga
    40981 tgcaccaagt atggtttcgc ctttacaatt gttcgaattg aatattgatg taacgaatat
    41041 agaagaatag ccgataaaaa ggtaaaaagg atttgtcgaa tacgcttctt ttgacaacct
    41101 tgatttaatt gaaaaacact ttataatgaa gccataacat ataaagcggg gcttttccat
    41161 gaaaaaaaca ttcgaaaatt gggcttatca tgattacacg accaagcttt cggtatctat
    41221 cgtttatgcg ttgatcgcat cagtcgcatt gaatttcttt tatcagccag gacatattta
    41281 ttcaagtggt attactggtc tagctcagat tttgacgact ttgtcaaaat cactgatagg
    41341 attcgaagta ccggtatcaa caactttgta tttactgaat atacctttgt ttgtattggc
    41401 gtggttaaaa atcgggaaga agtttacaat tttcactttt atcacagtca ctttgacctc
    41461 cctttgtatc catattttac cggaaacagt cctatctacc gatccaatca tctgtgcgtt
    41521 gtttgggggg gctgtgatgg gactaggtat tgggctagcg ttgagaaacg gcctatcatc
    41581 gggcggtcta gatatcgtga gtatcacgat tcgtaaaaaa acaggaaagt cgattggttc
    41641 tatttcgatc tacttcaatg cattgatcgt ttttgttgcc ggaattctat ttggctggca
    41701 atatatgttt tacagtacgt tgtctatttt cgtcagtgga aaagtaactg atgtcgttta
    41761 tactaaacaa aagaagatgc aggtaatgat cgtaacgaaa catccagaaa aagtagtaaa
    41821 tgaaatccaa aactgtatgc gtagagggat cacgatcatc aatgaagcag aaggtgctta
    41881 taagcatgat aagcagacag tattgatgac gatcgttaca cgatacgaac tgcctctact
    41941 tagagaagca atgaaagctt ctgatccaca agcttttgta agtattgcag aaaacgtcca
    42001 gatacttggt cgtttctatg aggatggcat ttagatcgag tttgacaaaa attcatgaaa
    42061 tactgctgta gacagacgtt cgtttgtctg cagttttttt ctaccatagt agaatcagca
    42121 aagaaaaact gcttttttat ttgaaatttt cttaaagttg aaaaaaagca tgtttatttt
    42181 tttaaattta tgggatgata gaaaaaaata gtagagaaag gacacgattc tacttgctat
    42241 tttcacttaa tacccgtata ttacgaatga aaaaagttta tgaaaagttg gaatattgaa
    42301 tggaaaaaca aaaaaggtta aaaaacagcc gctacatcac tgggtttgat gggctccgtt
    42361 cattagctgt ggtgggtgtt atcttctatc accttctgcc aactagtatg aaaggcggct
    42421 atttgggcgt tccgattttt ttcgtcgtat ctggttattt gatcacggat ctgctgcggc
    42481 aggaatggga acaaaatgga aaaatcaata tttggcaatt ttatgtcaga agaatgaaga
    42541 gactctatcc ggcacttgtt tttatgcttg tgacagcttc ggcttatatc actttgtttc
    42601 agcgagggct gttaaacaat cttcgaggag ttgtggtcag cagcttactg tatgtcaaca
    42661 actggtggca aatcaataac ggtttatctt atttcgatcg atttgctaat gagtcaccgt
    42721 ttacgcatat ctggtcttta gcagtagaag gtcaaaacta tttggtttgg ccgatcttgt
    42781 ttgtattgtt gatggtattt gttcgtaaaa gaaaatggat cttttatact gtgttaggta
    42841 catccttcct ctccgccatc ttgatgatga tcttgtatac accaggaggc gatccaaccc
    42901 gtgtttatta cggtaccgac actcgtctgt tttctatatg gttgggaagt gcgcttgcgt
    42961 ttgtttggcc aagtacgcgt ttaaagaaaa acatcccgaa acaagcgaaa aatattctga
    43021 atatcgctgg aggaatctcg cttttggcac tgattctttg cttcttcttt ttagatgatc
    43081 attacaattt catctattac ggaggaatgc tcctagtgag tctgttatgc gtagtgctag
    43141 ttgcagtgac agcacacccc ggagcaagtt tgaatcgatg gctgacaaat ccagtattta
    43201 cttggattgg caaacgaagc tatggcattt atctgtatca gtttccagtt atgatttttt
    43261 atgaagcaaa ggtgactgat attgccgatc atgttttgtt gcatacttta gtggaaatca
    43321 cattgattct aatcatcagt gaactttctt atcgatatgt ggagcgtccg ctagcacgtt
    43381 ttgattatgg tcgtacattc caagtgatga aaggatggtt cagcaaaccg atcatcagta
    43441 agagcaaacc atgggttgtt ccaggcacat tacttgtagc aattgcactc gttggtttcg
    43501 ttacggcacc gaaaaacgct gtaacagccg atcaaaaaca actgcaggca aaaatcgctg
    43561 aaagtaaaaa attagcagaa gaaaccagaa acaattcaga aaacaccact agttcggttg
    43621 accaagcgat ccttgataag tacgagttgt ccaaggcaca agggaataag gcacaaaaat
    43681 tagaactaac ggcatttggt gattcagtta tgttggatgc tgcaacggat ctacaagaac
    43741 tttatccaaa agtagtggta gatggagatg tcggacgaca attatatgcc agtgtaccgt
    43801 acattgaaaa attaaaggaa caagatttgt tgaaagacac tgttctgatt ggactaggaa
    43861 cgaatggctc attctcggaa gctcagtttg atagtgtgat ggatgcaatc ggtgatcgaa
    43921 aagtatactg ggtcaatgtc cgagtaccga cgaagcgctg gcaaaatgac gtgaatgcga
    43981 tgcttgaaca gatgacgaaa aaatacgaca atatgacgct gattgattgg tacagctaca
    44041 gtaatgacca tagtgactgg ttttatgatg atcaagtcca tccaaatcct gaagggatga
    44101 cacattacat tcacttagtc agcgaagcct tgctaggagg aagttcaagt acgaacagca
    44161 gtgagacaaa cgtgacagaa tgatagctac aaaaaacttc aggcaaaaac atttgttttt
    44221 gcctgaagtt tttttatatt taccgtgttt ttaacattca tatagattca ggaagcaaaa
    44281 cggttgagtg tgtctgtaaa taacgttctt tttgttttcg ggtgagtttt ttgaatgcag
    44341 cttctgcttt tgttgcttcg cttcttgaag aaaatacttc tgcatggatc atcttaacag
    44401 gtcttctttt ttgaagacga gtatatttag caccagtacc attgttatgt tcctctaatc
    44461 gtctagaaag atcggttgta tatccaccat aaaatgtctg atctttacag aataatacgt
    44521 aaaaataatg ttcatttgcc atagagcatc tcacgaatct ctgcagtgta ttctccatcc
    44581 atattgtaag tgatcaatgg tggagcgact ttgaatccgt ctaatttgcc ttctttgatt
    44641 ccttcaacta acaaaatatt tgcctctttt ccaggttttg ggtaaacaaa ttggatcctt
    44701 ttaggtgcaa ttcgatagtc ttccatgacc tgaatgattt ctaagaaccg atcaggacga
    44761 tggaccatag taaagcgtcc atttgttttt agtagcttac ttgaaatatc gatcacttct
    44821 tctaacgtcg tatgaatctc atgacgggcg attgccaggt gtttattagg gttcgtttta
    44881 tttgttggca gccctttgaa ataaggtgga ttacagacga ctaagtcaca agaattatgt
    44941 cgaacgacag ataagctgtc tttcagatcg atggtgtgga cagtcatttg ttcatttaaa
    45001 tggttaagcg cgatactacg tcttgccatg tctgctagac gaggttgaag ttcgatagcg
    45061 tcgatagccg cctgggttcg tttacttaaa aaaagtccaa ctgcgccatt tcctgcacaa
    45121 aggtcaacaa tcttcccttt tttaggtaca cgggaaaagt gagcaagcag cacagcatct
    45181 aaagaaaaag aaaacacttt ggaactttgg ataatcttga tatcttctgc gtataattgg
    45241 tcgattcgtt cgtcttcgtg aagcatggat tccctccaaa atgaaaaaaa tagtcgtctg
    45301 tattatacta tttttattaa aaagaaccaa ggtggcttgt caaacctcta ttttttatgc
    45361 atactatgac cataagaagg gaaaggaaga gactatgttt tatcgattta tgcgcggggt
    45421 agttcgagta ctattagcct tagttaatgg aaatgcccgc tatgagaata aagaagtttt
    45481 accgcaagat gagaattaca tactagttgc tccccatcgt acttggtggg acccattata
    45541 tctggcggta gctgcagcac ccaaggaatt cagctttatg gcaaaagaag aactgttcaa
    45601 aaatccagtt ctgcgattta tattaactcg atccaatgct tttccagtta aaagagaaaa
    45661 tccaggacca agcgtcatta aaacacctgt aaagattttg aaatcgacaa atttgagttt
    45721 gatcatgttt cccagtggga caagacatgc tacagaactt aaaggcggaa tggcattgat
    45781 ttccagaatg tctaaagcga aaatcgtgcc agctgtatat caaggtccgt tgacacttgg
    45841 ggatctgttc aagcgtcaga gagtgacagt gcgtttcggc gaaccaatcg acttatcaga
    45901 tatcaagaaa acggataaag aaggtatggc ggagatcgaa cgacgaacac aagcagcttt
    45961 tgacaaattg gacaaagaag tgaaccctga tttcaaatat gaagtcaaaa aaaaataaaa
    46021 tgataagttt atgcgtgggc ggtcttttga atctactgtc ctgttcagtt cagacttttt
    46081 caatgaaaac gagggagaga cgtcaagtct gccctctttt tgcgttccaa tatgtaaaat
    46141 agaagacagg tgtcatgtaa tggatcaaag tgttataata attagaagga cattttttga
    46201 ggtgattgaa tgcgtttttt acatacagcc gattggcata tcggaaaaaa actacaaggt
    46261 tatgacttga ttgaagacca gcgtcatgta ctatcttcta tactagatat cgcgaaagaa
    46321 gaaaaagtag aggcaatcgt gattgccgga gacttatatg ataggagtgt cccagctgtt
    46381 gatgccgtag aattatacaa tgaattgatg gtcgattgga atttaaagga aaagatcccg
    46441 atttttgcta tatcaggaaa tcacgatagc agtacgagac tttcagcggg tacgccatgg
    46501 ttcagttatt cgaactattt tttgcgaaca cagctagcag aagcatttga accagtcgag
    46561 tttaaaaacg tacaattttt ctttcttcct tactttgaac caattgcagc gagattatat
    46621 tttgaagatg acaatatccg aacgatcaag caagcggtag ccaaggtgat agataaaatg
    46681 caggagctat ttgattcaga aaaaaaacaa gtattgatta gccatttttt tgtaatggga
    46741 agtgtaaaat ctgattcgga aacgaaaatc gaagtaggcg ggttggattc gatccctggt
    46801 gaactactta caccgtttga ttatgtagca ctaggacatt tgcataatca agctgctttg
    46861 agacaggata atgctcgcta tagcggttcc ccattgaaat tctctttatc tgagatcaac
    46921 caaaaaaaag gcgtatggat cgttgacctg acaacaggcc tggagttgaa ttttagggag
    46981 atcgaacccc tgcatgatat tgaagaaata acagcgagtt tcaaagaatt gctggatcct
    47041 gccttttatc agtccgtcaa tcgggagaat tacatctata tctatctcac tgatagaacg
    47101 atcatcccga atatgatgaa ccagcttcgg caaatatatc ctcgtatttt ggggattgaa
    47161 cgggcaaacg gtcgagcaga acaaacaaag cgcaaacgaa aagtactaga aatgaaagat
    47221 cctcaaaaat tagcagaagc attttttagt gaaatgacgg aagagcagct aactgcaaac
    47281 caagaaaaat ggctcggaga aacgttgcaa gaaatcatgg aaatggaatg atcaagaatg
    47341 aaaccgaaaa aattacaact taaaaatttt ggtcctttta tcaatgagac cgtagatttt
    47401 tcacgtttga cagaagcacc gctttttttg atcagcggga agacaggagc tggcaaaacg
    47461 acgatttttg atgggatcac cttcgcactt tttggtgaaa cttccggaag acttcgctct
    47521 ggaaaagaga tgcgttcttt gtttgctaca ccagacgaag aaacaagcgt gactttttct
    47581 tttgaacatc agcagatgag ctatgaaatc gaacgaaaac cagaacaagt tttagcaaaa
    47641 cgaaaaggaa acggaacaaa aaaacaggca gccaaagtaa cgttgaccat ttttgatggg
    47701 aaaggaaaag aaataaaaca aatcacaaaa cgtacagaag cagatcagtt gatcagagaa
    47761 ctgatgaatc tcgatgcgaa acagttttct caaatcgttt tactacccca aggagaattc
    47821 cgtaattttt tggtttcatc aagcagtgat aaagagactg ttttacgtca tttattcggt
    47881 actcaatttt tccaacagtt caatgagcga ttaaaagaaa aagcaaaaca acagcaacaa
    47941 aaattggatc atttagagca agagctgcat cttctgcaaa aaaggtttgt tttagtcaaa
    48001 gaagaggaac cagcagcagg gtttgaagcc gttttagccc aatggacaaa ccatcaggca
    48061 atactcgaaa aacaaatcac ggaagaaaaa gaagctctaa cggaacttca ggaaaaacaa
    48121 aagcaactag aaaattccta ctatcgtttt gaaagcttga aaaaaagtta tgaagaaaaa
    48181 acgatattaa taaacaagca aaaggaatta gctgctcaaa aagacgaaat agaggaaaag
    48241 aaacgatgga ttgggtatta tgaatttacg caacgattag tcgaaccaat ccagcgccta
    48301 caagaaataa aagttgaaaa aatagaactg tcacaaaaaa gtagtgagca aacaaagaaa
    48361 ctagcgatga ctacgcagaa ttatgaagag tggcaaaaaa atgaagcaaa ccgcgtacgc
    48421 cgacaaacaa aaatcaaaga agctgctcaa aaactgcaaa acaaacagat gatggtgcca
    48481 attgtcgaat ccttacgtag taaaaaacaa gacgcagaac aattaacaat ttcattggat
    48541 aaagaagaat tgaatgccga gaagctgtat caaaatctcg ctatctatca ggaaagaact
    48601 cgaattcttc aagaaaattt actggatcag gaaaaactgc aagaagatcg tcttgctttt
    48661 ggacagttaa aatatctaga tgaaaagcag caagaaatag aaaaacaaaa agcaaaagac
    48721 actgggtcgc tcgcacagat aagagaagaa ctcaaaaaag caactgaaga aataacaaaa
    48781 aatagtcagc tattgaaaga acagacagaa caagcagaac agctgaaaag ccaatgggca
    48841 aagcagcaaa ttgctcgtct tcagcttttt ttgcgtccag gggagccatg tcttgtttgt
    48901 ggatcaacgg aacaccccat acaacaatta aaacacgaac agccaaataa ggaagatatc
    48961 ttaaaagtag aacaacaact ggcagaagct gagtctgctg tagaaagaac tcagcaaatc
    49021 attgccaaaa gtgaagcaca tgaaaataat cttcaacaac aattatcaga tttagaacaa
    49081 aaaatcatgg caacgaatat acaattggct gaacaagaag aacacttgca acaggctttg
    49141 aaaaaggcat ttcctgagat aacagaacaa aaaacaaaaa caactcttgt agtaatcgaa
    49201 caatctttag atagacgaaa aagcaagatg gccaatgaaa gaatagagct ggaagaaata
    49261 agacaacaag cagaaaagct cgaacaacaa ttagctgtct acaaacagca gcagacagaa
    49321 aaaaataatc agctccagca actgcaagga gaaatcaaaa gtttagaaca acaattgcaa
    49381 gatacggata gtaactgttt agaagaggag atacgacgac ttgaaaaaca gttagaagaa
    49441 gactcgaatc aactagctgt tgagcagaaa gcaggagtgc aattgttgca agatcttcat
    49501 acgcttcgga cacagcttga tcttcaacat gaacaaatag aaaaaataga tgaaaaaata
    49561 gaaaaggaac aagaaagaat agctaaaaag ttagaaagcc agtcgtattt ttcaacaatt
    49621 gaagaaatct gtgcatttgc acaacagcaa gcacaatata aagaaaacca acgtacagtt
    49681 acttcttatc atgaagagca attagtgaca gctgaccggc tgcgtcaatt agaaaatatc
    49741 cagctaactg agacattccc agatacagaa gagatcagcc aacagttaga agaaataaga
    49801 cgtatgatcg aaataaagca agaaaagata tacggtttac aagaacaaaa aattagtaat
    49861 caaaaaatct acgatgaatg tctttcgatt tatcacacaa gccagacgaa attagatgag
    49921 cttacgcaat tacagcagct ttcgcaaaca ttcaacggag aaaatcctaa gaagactagt
    49981 ttggaacggt atgtgcttca ggtttatctt caagaagtgt tacaagtagc aaatgatcat
    50041 ctccaacgtt taacgaaaaa tcgttatcaa tttgagctag cggataccat tggcagttat
    50101 cgagggaaaa ccggattaga aatcaatatc tatgatgatc aggcaggtat gtctcgaagt
    50161 gcgcataccc tgtctggcgg tgagagtttt attgcagcac tttctttagc attagctctt
    50221 gccgatgtga tccagacaca agctggtggt gtagcaatcg atgcattgtt tattgatgaa
    50281 ggatttgggt cattagatga agaagctttg gaaatggcaa tggaagcttt agaaacgatc
    50341 gaaagtgaag gaagaatgat cggcatcatt agtcatgtaa gagaattgaa agaacgagta
    50401 ttacaacaaa taagaattga tacagaaggt agcggacaaa gcaaaatacg ctatcaactt
    50461 ggataaaaag ggagattggt aactatgaaa tggagtattc cagaaaaagt cattgcacgt
    50521 ggacgtcaat acttgcaaga aggccgagtg ttatcggttg tccctgacca agaagaaaac
    50581 gtgtggcatg cagaagtctt gggcagtgaa ttgtatatgg ttgatttgga tggtacagca
    50641 aaagagatag attactgcca atgtccctat tgggaggaac ataaatattg taagcataca
    50701 gttgctgtgg aactttattt acggtcgaaa aatctatctc gtatcatgaa aaaagaccag
    50761 ccaatgctag aaccggtaaa agcaacaagt gagggagaaa tcattgcaaa aggtttttct
    50821 cgtctaaaaa cacaaaacaa tcctcaagcg gtgcaacctt tgattattga ataccaagta
    50881 gaaacgattg agactaatca gtatcaccca gagctttcta ttttggcact ttacttgcgt
    50941 atcggttcat tggcatcacc gaaaaaaaca tatatcgtaa aaaacatcta tgatttttta
    51001 caagcttaca cagaaaaaga aacttacact gttaataaac agtaccagtt tcgtctagat
    51061 aaacgggcgt ttcaaaaaga agatcaaaaa atactcacgc atcttgcagg aagtgctcag
    51121 acgcatcaat tattaggtac gaacggctta caagtcaaag gaaaactaga taaaagatat
    51181 ctgcttctcc cagtagaaca agcccgcttc ctgttagagc agatgaatca gactacacgt
    51241 ttgcatctgc agataaatga acaaaacgat caaggaatac aattttctga tcaaaacaaa
    51301 cctttatcat tcaaagtgga aaaatttgga gaagattatt tgctgcaagc attgaatgat
    51361 tttgattttt tctttatgca ttatcagtgg ggagttatcc aagggacatt ttattctctg
    51421 acgaaatatc aacaaacggt ttatcaaact tggaaacaat tgattcgccg attggagaaa
    51481 ccagaagtag tgttcaagaa aaaagaatta ccgtctttgt ttaaagagat tatccctctg
    51541 ctgtcagaga tcggcgacgt agctattgcc cctgcagtag aagaggaagt agcagatatc
    51601 cccgtcgaat ttattttctt tttccgaaaa gcaaaaggaa aaatccaagc tcgagtcgat
    51661 ttcgtctatg gtgaagtcat ctattcgact gacccgaagc atgaggtctc gacagaacag
    51721 gcttttcagg tgttacgtga cacagtagaa gagaaacgag tacttgatct attcagaatg
    51781 tatcgctacc agaaaaatga aactggttat gaacgtccat tgccaagcgg ggaagaacta
    51841 tatgcctttt tccgcacaga actatctgtc ttccgtcagt ttggtgaagt cagacttgga
    51901 agaaaattgc gagagttgta tttagatgcg cataaacatc aaccgaaaat cgaagtaacc
    51961 gagaccggtt catggctcga tattcgattc gatgtgacag ggatagagga gcaagaaatc
    52021 gatcatgtcc tgcaaagctt gctgagaaat gatgcttttt atacattaga aaatggtcag
    52081 gttctttctt ttgattcaga agaatttcaa caaacaagcc aagtgttgca acaattacgg
    52141 gaatcgatac gtacagaaga aggaacgatc catgtaccta aaaatcaagg attgatcatt
    52201 caaaatcaat tggaaaaaag caatgctact tttagtgaat cgttccaaac gatggtacaa
    52261 gatctcattc atccagaaag ataccaggca cagcttccca aaggattgaa tgcaacgatg
    52321 agagattatc aaaagcaagg ttttcgatgg ttgaagatgt taggtcatta tcagtttggc
    52381 ggtattttag ccgatgaaat gggacttggg aaaacactac agacaattgc ctttttactt
    52441 tcggaaaaag aagaacggaa aagcttctct gctttgatcg tagcgcctgc gagcttaatt
    52501 tacaactggc aggcagaggt gagaaaattc gccccttctt taagtatcca agtaatcaat
    52561 gggaataaaa aagaacgtga agaattactg gctaaagaca cagatatccg agtgacttct
    52621 tatgccagct taagacagga tctagctaat tatcagtcgc aaaaaattga ttatttgatt
    52681 cttgatgagg cacaaatggt caaaaatagc agtacgaaaa ctgcacaagc attgagagaa
    52741 ttagctgtac ctcagcggtt tgcactgagt gggacaccaa tcgagaataa tttagaagaa
    52801 ttgtggtcat tgtttgcaac gattatgcca ggatttttcc cgactaaaac aaaattcagg
    52861 gaacttgccg cagaagaaat tgcgcaaatg attcgaccgt ttattttacg gcgagataaa
    52921 caaactgtgt tgaaagactt gccggaaaaa acagagatga atctatattc tgcacttaca
    52981 gaagaacaaa aaactgttta tttagcttac ttgcgtcaaa tgcgtgaaga aatcacatca
    53041 atggattctg aagcattcaa gaaaaaccgg atcgggatac ttgctggtct gacaagactg
    53101 cgacaaattt gctgcgatcc tcgtctgttt attgaagatt accaaggcgg ttccggcaag
    53161 ctcgaacaag taaaagatct actggtagct gccaaagaga acaaacgaag agttttgctg
    53221 ttttctcaat ttacagggat gctgactatc ttgcaagaag agttagccga gctagggatc
    53281 tcgacatttt atttaagagg cagtacgaaa ccacaagatc ggttaagtat ggtagacgca
    53341 tttaatgctg gcgaaaaaga tgtttttctg atttccttaa aggctggggg aactggattg
    53401 aatctcacgg gagcagatac ggtcatcttg tatgaccttt ggtggaatcc tgccattgaa
    53461 gaacaagctg ctggacgtgc acatcggatc ggacaaaaaa atgtggtaga agtgtggcgt
    53521 atgatctctg aaggaacgat tgaggaacgc atggattcac tccagcaaga aaaacgcgaa
    53581 ctgttccaaa aagtgatcca aggtaatgaa gaacagttga cgaaactgac tgaagaagac
    53641 atccgcatga ttttaagtgt aggagaaatc gaggagtaag agactcgaaa aagcgttcag
    53701 taatgggtaa gaaagccttt ctaaagaagc gattgtttat ttgatttaaa aaatagcaag
    53761 gaggctggga ttccatttta tcccagcttc cttgctatca tttttttatt tctattttta
    53821 aaggttatac aaagattgtc ttcattgtct ctaacagctg atcaaaagaa gaaatctcga
    53881 tatcgcaaat tggagtctgt tcaattttgc gttctcgatg gttgaaccaa agcgcttgcc
    53941 agttagcact ttttgcacct agaacatcat tgtcgtaatt atcgccaacg tataaggtct
    54001 cttctggaag catatgaaat tctttctcag ccagctgaaa aatctctttt tctggttttt
    54061 ggaaacctgt tgcttgagaa atgagcatat tgcgggaagg aatccaattg ttcaattgaa
    54121 gttgcttcag tttttttgtc tgatggtcag tcggcccatt tgtgataatt cctaatggga
    54181 cattcttttc cttcaagaaa tcgagggttt ttttgacctc ctcatgcaag cagatattat
    54241 caagttcttc ctcgtaaatt ttttgaaata agaggccatc ttcttccgta atatgagggt
    54301 agtctaaatc ttttagtgat tggctgatcc gatgtgctcg catatattct agtgtccaat
    54361 ctcctgccat tacttttggg aagttttcat cactatgatg gcggaaccgg atatatagtg
    54421 gatgcatatc tgcatctgaa acaagcggaa cgactctttt gaccgcgttt cgaaatggct
    54481 gctgctgatc atacatcgta tcatcaacat caaacacaac tgatttaatc aaaattttac
    54541 acgcctttcg gttttcattg attttttcac aaatccttga ctattgtaac agaaaaaatt
    54601 gtctaaaaaa gctgataaat aaaggttttt cttactatat ttctatttcc ttgacagaat
    54661 aaggttttta tatttcagtt tttttcacta aaaatcgtga aacacgtcaa taagtttgat
    54721 gaacaaatta atcattatgg tagaagtcga ttgatttatt ttaatgcaat ctctgtggaa
    54781 ttttcg
//
LOCUS       DAJSAW010000014        54418 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJSAW010000014 DAJSAW010000000
VERSION     DAJSAW010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.014"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(2..913)
                     /locus_tag="ONT64_000927"
     CDS             complement(2..913)
                     /locus_tag="ONT64_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR2302360.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1370..1798)
                     /locus_tag="ONT64_000928"
     CDS             complement(1370..1798)
                     /locus_tag="ONT64_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302361.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(1826..2464)
                     /locus_tag="ONT64_000929"
     CDS             complement(1826..2464)
                     /locus_tag="ONT64_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2302362.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2468..4450)
                     /locus_tag="ONT64_000930"
     CDS             complement(2468..4450)
                     /locus_tag="ONT64_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HCR2302363.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4630..5295
                     /locus_tag="ONT64_000931"
     CDS             4630..5295
                     /locus_tag="ONT64_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2302364.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(7230..10379)
                     /locus_tag="ONT64_000932"
     CDS             complement(7230..10379)
                     /locus_tag="ONT64_000932"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HCR2302365.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(10392..11609)
                     /locus_tag="ONT64_000933"
     CDS             complement(10392..11609)
                     /locus_tag="ONT64_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR2302366.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            complement(11599..13194)
                     /locus_tag="ONT64_000934"
     CDS             complement(11599..13194)
                     /locus_tag="ONT64_000934"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCR2302367.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            complement(13704..14486)
                     /locus_tag="ONT64_000935"
     CDS             complement(13704..14486)
                     /locus_tag="ONT64_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302368.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(14551..15387)
                     /locus_tag="ONT64_000936"
     CDS             complement(14551..15387)
                     /locus_tag="ONT64_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2302369.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(15405..16814)
                     /locus_tag="ONT64_000937"
     CDS             complement(15405..16814)
                     /locus_tag="ONT64_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2302370.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(16828..18651)
                     /locus_tag="ONT64_000938"
     CDS             complement(16828..18651)
                     /locus_tag="ONT64_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2302371.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(19494..19820)
                     /locus_tag="ONT64_000939"
     CDS             complement(19494..19820)
                     /locus_tag="ONT64_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302372.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(19866..20228)
                     /locus_tag="ONT64_000940"
     CDS             complement(19866..20228)
                     /locus_tag="ONT64_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR2302373.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(20265..20480)
                     /locus_tag="ONT64_000941"
     CDS             complement(20265..20480)
                     /locus_tag="ONT64_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2302374.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            20607..20942
                     /locus_tag="ONT64_000942"
     CDS             20607..20942
                     /locus_tag="ONT64_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2302375.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(21018..23015)
                     /locus_tag="ONT64_000943"
     CDS             complement(21018..23015)
                     /locus_tag="ONT64_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302376.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(23113..23388)
                     /locus_tag="ONT64_000944"
     CDS             complement(23113..23388)
                     /locus_tag="ONT64_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2302377.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            23620..24354
                     /locus_tag="ONT64_000945"
     CDS             23620..24354
                     /locus_tag="ONT64_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2302378.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            24393..25535
                     /locus_tag="ONT64_000946"
     CDS             24393..25535
                     /locus_tag="ONT64_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2302379.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(25630..27021)
                     /gene="sufB"
                     /locus_tag="ONT64_000947"
     CDS             complement(25630..27021)
                     /gene="sufB"
                     /locus_tag="ONT64_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR2302380.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(27026..27496)
                     /locus_tag="ONT64_000948"
     CDS             complement(27026..27496)
                     /locus_tag="ONT64_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCR2302381.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(27483..28718)
                     /locus_tag="ONT64_000949"
     CDS             complement(27483..28718)
                     /locus_tag="ONT64_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR2302382.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(28715..30001)
                     /gene="sufD"
                     /locus_tag="ONT64_000950"
     CDS             complement(28715..30001)
                     /gene="sufD"
                     /locus_tag="ONT64_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR2302383.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(30014..30787)
                     /gene="sufC"
                     /locus_tag="ONT64_000951"
     CDS             complement(30014..30787)
                     /gene="sufC"
                     /locus_tag="ONT64_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR2302384.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(30984..31832)
                     /locus_tag="ONT64_000952"
     CDS             complement(30984..31832)
                     /locus_tag="ONT64_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR2302385.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(31856..32542)
                     /locus_tag="ONT64_000953"
     CDS             complement(31856..32542)
                     /locus_tag="ONT64_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2302386.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(32535..33569)
                     /locus_tag="ONT64_000954"
     CDS             complement(32535..33569)
                     /locus_tag="ONT64_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2302387.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(33632..33888)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(33964..34302)
                     /locus_tag="ONT64_000955"
     CDS             complement(33964..34302)
                     /locus_tag="ONT64_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCR2302388.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(34304..34660)
                     /locus_tag="ONT64_000956"
     CDS             complement(34304..34660)
                     /locus_tag="ONT64_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCR2302389.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(34666..35853)
                     /locus_tag="ONT64_000957"
     CDS             complement(34666..35853)
                     /locus_tag="ONT64_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR2302390.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(36044..36889)
                     /locus_tag="ONT64_000958"
     CDS             complement(36044..36889)
                     /locus_tag="ONT64_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2302391.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            37123..38088
                     /locus_tag="ONT64_000959"
     CDS             37123..38088
                     /locus_tag="ONT64_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCR2302392.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            38465..39508
                     /locus_tag="ONT64_000960"
     CDS             38465..39508
                     /locus_tag="ONT64_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HCR2302393.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(39570..40034)
                     /locus_tag="ONT64_000961"
     CDS             complement(39570..40034)
                     /locus_tag="ONT64_000961"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR2302394.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            40280..41227
                     /locus_tag="ONT64_000962"
     CDS             40280..41227
                     /locus_tag="ONT64_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR2302395.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(41438..43510)
                     /locus_tag="ONT64_000963"
     CDS             complement(41438..43510)
                     /locus_tag="ONT64_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR2302396.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(43443..43820)
                     /locus_tag="ONT64_000964"
     CDS             complement(43443..43820)
                     /locus_tag="ONT64_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCR2302397.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(43949..44527)
                     /locus_tag="ONT64_000965"
     CDS             complement(43949..44527)
                     /locus_tag="ONT64_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2302398.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            44659..45951
                     /locus_tag="ONT64_000966"
     CDS             44659..45951
                     /locus_tag="ONT64_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR2302399.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(46011..47129)
                     /locus_tag="ONT64_000967"
     CDS             complement(46011..47129)
                     /locus_tag="ONT64_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HCR2302400.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(47143..48564)
                     /gene="arcD"
                     /locus_tag="ONT64_000968"
     CDS             complement(47143..48564)
                     /gene="arcD"
                     /locus_tag="ONT64_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCR2302401.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(48810..49931)
                     /gene="mnmA"
                     /locus_tag="ONT64_000969"
     CDS             complement(48810..49931)
                     /gene="mnmA"
                     /locus_tag="ONT64_000969"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR2302402.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            50335..50934
                     /locus_tag="ONT64_000970"
     CDS             50335..50934
                     /locus_tag="ONT64_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HCR2302403.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(51072..52358)
                     /gene="efmA"
                     /locus_tag="ONT64_000971"
     CDS             complement(51072..52358)
                     /gene="efmA"
                     /locus_tag="ONT64_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HCR2302404.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(52629..52973)
                     /locus_tag="ONT64_000972"
     CDS             complement(52629..52973)
                     /locus_tag="ONT64_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HCR2302405.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(53110..54255)
                     /locus_tag="ONT64_000973"
     CDS             complement(53110..54255)
                     /locus_tag="ONT64_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR2302406.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
BASE COUNT        15827 a        11239 c         9281 g        18071 t
ORIGIN      
        1 cttatttact acttgctgta ctagaatctg aagtgaatgt actcaaaaca ttttcaaagt
       61 ctttgtcttt gatttttaca ttagcgtctt tcaatacttc gccaattact ttattctgga
      121 aggtactgtc acttaatttt gtgtctgttg caatttcttt caattctttt ttgtatttat
      181 ccatgtcgtt gcctttgttt tggtttttca ccattttaac cacataatat tccgtagtat
      241 aagttgaagc attggtagaa gtgatcacat cagaaacttg accatctttc aacttaaagg
      301 ctgcttcttt gacttctgcc ggaaccgttg tagtagtgga atcaaatttc acttttccgc
      361 catcttcttt tgtcgtagta tcagttgact tgtctttggc taatttagag aaatcgtctc
      421 ccttatcagc agctttcttc acatcttttg catcatcttc acttgctact tggataattt
      481 gagcttccac ttctggatgg aaagattccc aagccgtttt taaatcttca tcggtcaatt
      541 tgatatgttt ctttagacct tcttgaaagg ctagactttg tttgatactg tctttgaatg
      601 tttgttctgt gtaacctgca gatttcaatt gtgaatcaaa tgaatcaccc aattgtttct
      661 ttgtttgatc atattgagaa tcgatttctt tggttgatac gtcatctcca tatttatctt
      721 caaatacttg ataaacgatc aactgttgca cagcggaatc tgctccttgc atttttttca
      781 agctatccat tacttcttgt tcgctgattg taccgccttt ataactaacg gctgtttttt
      841 caccggcatt cgtacagcct gacaataagc cagtgcctag tacgatggca agtgctaaaa
      901 atcttttttt catttatcaa ttctcctact tttcgttttt tgcgaattgc taaaaacaaa
      961 ggtgttcaag atgaaaatca tcaagaacac ctttactaat ctgctcgata cgtcctgaat
     1021 agatataagg ctacacaatc cagaaagttc gctcagcaga ataaatgtca agtagcacat
     1081 cacaccttaa tatactactt cttttgttac cgtttttcaa gtctatcttg cgttccgtat
     1141 ttaacgtaac taattcttag aacaaatgtt atcgattttg tttttcttct tccgctgctg
     1201 ggatattcaa atcagacact tttatgttcg attttccaga catttttttg ttgtttttat
     1261 tatgatatat ttttatacaa ctaaaaatga atatattaaa actaccaaaa ataactttta
     1321 acgctgtttc attatatgtt ttttctattt tatataaact tttggatact taataccaac
     1381 cggttggatt gtaccaacat gagaatgtgt cagttctatt tcctgtcgca cttgcatagg
     1441 cccatactcc ttttgttgca tttgctgatc gaatagagtt tccaactttc gcttttgcac
     1501 cgtgacgatt atagacacga ctatagtggt tagaatgacc tgtttttctc gtacaagttg
     1561 tatcactata ataaacccat cttccagagc tatgattaat tgctgttcta gaaatttccc
     1621 ggtctactgg taaatttgac ttaataatat tacctttagc atcaaaatga atctctcctg
     1681 gctttaaaga tttgtaatag atttcaccgt gaactgaatt tgcttccact tcagatgaca
     1741 tagcgtttcc tccaacaaaa cctatcccta gtgcacaaat aataactgct tttttcataa
     1801 aacgacatcc tttccttttt gatttttaaa gcatgatctt cttgtttcct attttggtca
     1861 gatatggatc atgcgtgact acgataacta acgtaccttg ggaagcttgt tgcgataaga
     1921 tttcaactac tttatcctta tttcttttat ccaatgaccc agtcggttca tcggctaaaa
     1981 tgattttgct attcttcaat aagactctcg cacaggcgat tctttgcttc tcaccaccag
     2041 ataatgtgta gataggcgta cttagcttct tgtttatatt tacttttttc aatgcctcat
     2101 actttgctgt tcgcttatct gaaacattta aattcgaaaa cttcaatcca atatctaaat
     2161 taaaatcgat tgtttcatat tcaatcaacc caaaatcttg aaaaagaaaa gatatggtat
     2221 ttctttttat caagcgcttc tctctcccag ttaattgttt cgtctcttga tcgaacaaat
     2281 agaattgtcc tgaactgaac tgatcattca atcctaatat attcaataac gtacttttac
     2341 ctgctccact ttctccaaca acagtcacaa gatccccagc atccgcagtg aaacgatcca
     2401 aatgaagaat cgttttgttt cgaatgattt ttttcgcacc ttttatcact atcaacggct
     2461 ccaattgtta acctccattc atcagcaata actgactttt cttttctgta gacaaaataa
     2521 aaacaactga aagaataaaa tcaatgaagc taaagaaaaa aatattaaaa agatatttag
     2581 gctctctttc tataagaaaa aggaagatta cacttgtcgt tccccatttg atgaggttca
     2641 tcatgaagaa atctttatac gtttcaaaga gcgaataacc acttgaacga aagatagcga
     2701 ttttccgaca attgtagaca aaatatatgc ataacatgac atagctcaac aagaagaaag
     2761 aaaagaaagt gagtaaaaat atctgtatca agtaatgaaa tgatctgctt aacactttga
     2821 cagctatctc ttcagtctga gtatacggaa atgataactg caagttatca cgtaactgac
     2881 tttcttcaat cagctcaaat aacctttctt tcgacaaaga gcctatcttg atctttaacg
     2941 gtggatagat ttctccgctg agaatatttc tttccaaata catactgttt ttcaatgtca
     3001 gtatttcaac aaccggataa tgttcgatcc aggggataga aggaataaat gtataaatgg
     3061 gttgattgtt ttttataaat agaaatttcg gttctttttc actgatttct tgataatatt
     3121 ctgttatttg cgccaggtca ttttttaaat ctgtatgtct atatcgttca gggatcaata
     3181 taacagggtg cgtttctttt tcggttattt ggatcaacct tccagagaca tcaatcaaag
     3241 gaaatttctt aagataattc agatttattt ttatccctct gccaaactgt tcattttctt
     3301 cattcaagta atctgataca ttgactaata tacttccgtt tttattcaaa gtttcatata
     3361 attcctgatc tgctgctgat gcaaaacgat tggaataaat aaaatccacg tggtttttcc
     3421 cgatggttac tggataaaat acatgatagt cctcaccagc gatttctttt ggtggttcat
     3481 gggaaacagc taactgtgaa gaagaaaaaa cgatagttgc taattcgaca ttggcaacgg
     3541 tcaaaagaag agaaaacaac aatccaaatg taaaaagaat gtattggata tagttgaacg
     3601 cttctcttaa cgaagcgatt ttttcaacta ataaaagaga aatcaaagag ataccatata
     3661 ttccgctgaa caagaaaacg tacatctgga acaaataaag aaattgctcc ttataataca
     3721 taaaaataaa accaccaatg attcctgcaa ttatgaatgt aataatgatt ttctttttcc
     3781 ccaataaaca ccacaatact tgatgtaatg aaaagtcatt attcaacaat aaactgattt
     3841 ctttattcat caataagaaa aaggatgata acaaaaggaa taaaagaatc aatccaatag
     3901 ttaaatacac tcgataatta tatacgtctg tatttgaaat aaccgaatac gaatagctat
     3961 tttcaaagtc ttcaaaaata ttagtggcat aatcagtgtg aaatgcttga ttgaatcgat
     4021 cagtgaatag tgaaatgaac cgttcatagg cttctttttc tctcgtcatc aagaaaaatt
     4081 caccttcaaa atcttttatt ttgatcgatt cttcaatgga cgttacgcga aaggaggtat
     4141 tagaataggg taccttctcg tgagaagatt tgacatcccc ttgataatac aatgtcattt
     4201 caagctttgg ctgtatcttg aagttccaag atgtatctac ttcattggat tcatttactg
     4261 tttttttcat ataagaaagc tcagtttctg atgagacatt ttcaaatacc atgattagct
     4321 gctcatattc ttcttgccca ataaaatgtt gtggcaatcg aatcggagta taagtatctg
     4381 tttgatggaa aaattcattt gtccgataga gtacctgttt atttatacta accaaggcaa
     4441 aaccactcat gataccggtc actatgattg tgagtaaaac tgtgaacttt tttttgatta
     4501 tcaccactct cccttactta aatgattctt tgataaaatc ataccgtttt tcatcaaaaa
     4561 aacgtatcta tcaagtgtat aggtcatgta gaataaatct atatactgta gaatcgaaag
     4621 gagaagaaga tgaacaccgg agaaaaaata cgaacttacc gcagaaattg taagcttacg
     4681 caaaaagaat taggcgaaaa aattggtgtt tccgataaaa cgatttctag ttgggaaaat
     4741 tctcgtacaa tgccagacct tgaaatgctg tcacttttac atcatacttt aggtctgccg
     4801 attgattttc cttctattga atcagctacc gaatcaatag aaccaacatt gccagatgaa
     4861 cctcttgtta acaagaaatc ttattcatca aaacaaccct tctcactcaa aaagacacta
     4921 tctttctttc tgctcgtctt ttgtttggtc tatctttttc ttatcaatta tcaatacaaa
     4981 aatgaaacga tcgatcgatt caatcctttt ttgaaaccac aaatcggcta taccacactc
     5041 cctgtaggat acgtggacaa ttccatcgaa cgttgggtat gtaatgatat ctttggccat
     5101 ggtacatggc tgtcttttca cgctggagag gtagaaaaag agaaaaacca tgctgtaatc
     5161 aaacacaaag gaaaacacgt catgaacgtt cgcctgctca cttgggaagc cattccctca
     5221 gattaccaag aaatcctcag ccaagactat gacgacgcct taaaaagaag ccctcaggag
     5281 ccagataggc attaagtagg agatgaacgt agagatagaa aaaatcaatg cacatgatct
     5341 tatccaaaaa acaggggtac aaattaattg aaaaagcctt gagatgaaag ttcctaaata
     5401 aaaaagttca gaatgatagg tatttctggc ttatagtata atatttacgc cacagcctcc
     5461 caatgattct ttttggaatc ataatcaagg atggacattc acaaaaatat tttgctcatt
     5521 cgatccgaat agattaacga ccaatctagt aagtaatata gattggtacg tagtcaagct
     5581 gcacataatt tagatcaatt ttagttcttt tttatatttc tatgatctgc tcatttactt
     5641 ttatatgatg gcttacagta acataaataa atatgataat cgctggtagt ttatccaaga
     5701 gtactcaata aaatacaaaa tggattgttc aagcgcctat cataatgata aaatgaaaca
     5761 aataaacgat acaaggagga agagactttg agtaatggca gaacaatttt ttcattgtaa
     5821 aaattgttca gttgttaatc tatgggattt cggtagcatg ggcatttttt tatagaggaa
     5881 tcacaccaac ctggccgttt tatctcttgt tagtgggaat attagtagga aattttgccc
     5941 ctcttcacta tcgtacgaac ttttttccaa ctgattctgg cctttttctt aaaacaaaga
     6001 cgcgaaaatt agaaaacatt attgaaatga caattacctg tataatcatt ttcttagtta
     6061 tattcctaaa cgtcttctaa taagaatctc ttaaaattag cttgttacag ttagttacct
     6121 agtttatata acacagttat cttatcttcc ttatcaacat cttttgccaa ctctttgtct
     6181 taaattcgaa acatataagc agagctacga acaccaagac tgtttcggct ctgttttttg
     6241 catcaaattc actcttatat agctgataat ctcggctctt taaaagaaat tattttcgct
     6301 tcattttatt ataaaaaggc taggaacctt gatgttatca ggttcctagc ctttatttaa
     6361 gtattacccc acagaccctt ccatatagat ttttaaatta cacactagtt taaactaaaa
     6421 caaacaagga aaaacttacc atttaatcaa aaaattagct catatgaata tattagaatg
     6481 taaattagac tggtcaaaat ctggtcaaaa aagaggacga atacaggtag tcctcttttt
     6541 tgttattttg atattctaaa tcatttttta ttggattatt cccttgttcg atatagaccc
     6601 aaattgatct atcaataatt atcaaactga gtcatataga gacaaaaatt tcaaaaatga
     6661 cctgttaaat ggtcatcgaa tatcatttaa aatcaatacg acggagtcag acacggagtc
     6721 aaaacaagcc cgctgagagt aaaaaatata cagtcccata ggtaaagtgc cacaggactg
     6781 tatatttttt tatttgagat tacctgaaat gattccattt aatttttatc tcattttttc
     6841 ctactagtta gtatcaaata ctctcctatt ttctcaataa actattttgg gttacatggt
     6901 atctgaaaca gctaaaacct tgtcattata gcaaaaaatc actttatctc cgtttcattt
     6961 cagatacatt tcacttaagc ggattccaaa aaaacaaaag aaaaaggaac ccctagttag
     7021 cctatgcttt ttattggttc ctctcaaaac acggtcatat aacaacgggg tggggtaaat
     7081 gaatgaaaaa ggtggaggaa acattattca atagctgtct taaatacttc gttataaaat
     7141 gtgaacaaat aaaagacatc tatccctatt ttctacactt ccacttgact accatctttg
     7201 aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt cattctcaag tggaatcaac
     7261 tcatcatcta acattttacg ccatgctcgc ttcatttctt gtgggtattt aaatggcttc
     7321 gcttctggat gtttggcttt atatccttcg taatccttac tgttgataat tgctttcata
     7381 ttaggaattg gatcttcacc tggtagatat tgcagattag aagcatgtaa ttctttctca
     7441 gaaacaaacc actcattgga aaagacatga atcaaatcat cactcgcttg tgtaaaatag
     7501 cgacgtttaa tgacaaagat atcttcatta ctatctaaag tccccacatc aaatgcattt
     7561 aaaatttgtc ggtaaacttc tttgactcct tctgttttat tcaatttagc caaagctttt
     7621 tcaatttctt ctcgtgcatc tgaactattc gcagcataag ttcctagtaa ttgatcaata
     7681 taagttgaat caatatcata caacttagtg gtattttcac tataaaattc gatcgttgaa
     7741 aaatcttctc cttctttttc tggttcttcc tctattaatg aacccttcac ggtctcgtac
     7801 acgccaattt gttcttccaa aagttgaatt tgattgttca aagttggaga tttcaccatg
     7861 tcatcattgt aatcgtcata agtcactaac gattcataag cacgattcaa ttcttgaaat
     7921 gctttgacgt actcgatacg gatttcaagt ggcgtatgtt catcaatgga ttccatggat
     7981 gaaacctctt ttaacttacc atatgcttgt ttgaatcttt ttttggattc ctgataactt
     8041 ggataaatca aacctgattc ttcttttgct tttgaataca gttttgtcgc atcctcgaca
     8101 ttcttctcca ttgtcgctgg cttacgaaac gtcacaatca atccttttgt cttatcagga
     8161 tacgtacgat tcgtacgtga aaaagcttgg atcaatcctg catactctag attacgatcc
     8221 acaaataggg tttgaatcgt aggggcgtca aaacctgtta atagacgatc cactacaatc
     8281 gctaaatcga tttgtttccc aaattgtttg aattccgctc tttttcgtgc caaacgatta
     8341 ttgatgtctc cgttataacg ctcaatgtct gctaatgacc aggaagtatc ataatactga
     8401 ttatattcct caataatttt ttgcatttct tcttgttggg cacttgaatc tcggctattt
     8461 tcttctaatg aataggtaat agcgattcgt ggaaaatcag gatcctccat cgttcgtcct
     8521 tcacgaatga gctgattggg aaactctttt gttaaccaat caggctcttt cgtcatctct
     8581 ttgatggccc gataatactt tttggccatg tcaatggaac tcgtggttaa gattgcagat
     8641 ttcgttggcc tgccatttcg aaaatcaaat tttgtatagg cattatctgg acggaacatt
     8701 ttatgaatga ctttttggat atgttcatct gcttcaaaaa cagctgggtc caaataactt
     8761 tcttttttga caggttccat ttgatcaatc atacgattga tctgttctga tgaatactct
     8821 gcatatgttt ctacttcttt taatttccga tagattttat tctctagtga agtcggctcc
     8881 acggtgtttt catgctctac ttgaaatcct aagacagagc catcttctag cgcattctta
     8941 atggtatagg tatgcaagac ctctccatac tgatcatggg ttgtacgtgc caattgccct
     9001 ttggcttgtt tccgattctc aggaaaaatc ggtgtacctg taaagccaaa ccaagtggat
     9061 ttcggaaaca tttttttgat ttcttccata ttttcagcac ttaacgctcg atgacactca
     9121 tctactacaa acacgatatg ttggcccatt aatttttgga agcgattggt tccttttttc
     9181 tcttcttgtt tctttgcata tttcaacgcc gcatctagtt tttgacgggt tgtaatgatt
     9241 acaacatttg cattggcatc tgccaacagg gtttcactta attctttggc acttcccgtt
     9301 ccaacgatca agctattcgc tttggcgttc ccggaagaaa tcccagtatt aaattccgaa
     9361 gcaaattttg taaactctgt ggtggtttga ttatctaaat ctttgcggtc cactaacatg
     9421 atcgttcgat cgatccccga ttttcgtgct aaaagcttag tagatacaaa actagtcaac
     9481 gtctttcctg aaccagtcgc atgccacaca taccctgatt gatgtttatt tgcggcaata
     9541 aacaaggctt gaatcgcatg tacttgataa ggatgtaaca ccatgagggt tttattgtct
     9601 tgatcttcac tgacgattgt ataatccgca atcaaacgat gagcatccgg gatatttaac
     9661 acttgtttac aaaactcata taagttttct actttttcat tgtctttcgt tcgccaacta
     9721 aataaaaact tagggtgcaa gtctttcggt aaggcatttg caaaataccg agtggtttgt
     9781 tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat tgttccgaaa cataccttct
     9841 tctgcatatt ttttgatttg gttaaatgcc tgataaacac catcttttgc agttacttgt
     9901 ttcagctcga tttggacaat cggtaatcca ttgattaata aggtgacgtc aaaacgtcga
     9961 tcccgtgctt ctatgttact tccacgtttt gcaatttgat ggaccacttc ataagtagag
    10021 attcctccac caatgtcttg attggaataa aggataagtg agacagaacc caattgcgaa
    10081 tcttcccgtt caatcgtgat ccgagccatc ccattttcac cttttaacca tttcgcagca
    10141 tcaaatgggg ttttcgttcg cagcagtaat tccgttttga tcgtttcaaa ttctttatcg
    10201 gtcaaaggaa aatcattcag ttctgcttga ttatttgaga taattttttg gcgtaaattg
    10261 acccaaaggt cttcttctga ttttagatcc ggacgatacg tccactgatt atgcccttct
    10321 ccaagtacac gaatcaactg ttcttcgact tctgcttcct cacggtgact aattttcatt
    10381 cttcttccct cctagacaaa catcttttgt aagaagcttt ttttcgtttc ttttagtaaa
    10441 tctaacttac gttgatgaag agcgatcgta tcatctagct gtttgaagaa agtaccgatt
    10501 ttttgttgtt catcctttac aggaacctta acactgatat tctcaatatc agaactgtta
    10561 taagaaggca atgcccctac ttgggagtat cttggcaaat ttatagtttc aaacaatatt
    10621 ttaccaaaat agatatccca agtattatta aataaataag ccatggtatt gttatctatt
    10681 aagaaagggc ttgtgaccaa acgttttcta ttaagcatta ttgctgcgcc aactttagca
    10741 aatataatcg ctggcacatc ttttattggc ttccattttt tacgctcaat ttgctcatta
    10801 gatacataat gatttgcatt tctcatttca tactcattac caatgttatt catgtcggaa
    10861 actttaaaaa atggagtacc ttcagaacca ccttgctcgg catcaggaaa tccaattccc
    10921 gattgagttt tacctaattc tcccaactta cgctcttccc aatcttccgt aaaaccagga
    10981 aaccggattt ctggcacttt tgctccgttt tcagggaaca ttttttgtaa gaatcctttt
    11041 ttcgtttcct tcagcaaatc taacttacgc tgatgaagag cgatcgtgtc gtcaagttgt
    11101 ttgaagaagg tgccgatttt ttgttgttct tcctcactag gaaacattgc tactatattt
    11161 ttaaactttt ttagagacag aacatgttga cctgttccct ctgcttgaat agataattgt
    11221 cgttggattc cattgttaat acgtcttaag aagaagtcgg aatcagcaga ttttaaagta
    11281 aatacaggat aaaaatagct tataatccct ctatcaataa tgtcattacg gttaaacaca
    11341 aacacatcat tatcacttcg actacggaac gtgaaatatc ctctaggtag tacaaagtaa
    11401 ccaatattat tttctgtagt tacttgccga tttgcaaaat agtcttcttg caaaactatc
    11461 cctttttgtt gagatgaagt tagcatagga aattgatttt gtacacttgt cttttcaaca
    11521 taatctacaa cgaagtcccc caacttcctc tgttcccaat catctgtaaa tcctgggaaa
    11581 cggatttctg gttgtgtatc attgctcatg tttgaacacc tccagcgcac cttgtaacca
    11641 cgcttcattt tcaggtgttg tttgcaggga agagatagct tctaaaagtt ttttttctag
    11701 cacttgctgt tcttgtcgta tcttcttgat gtcatttccg acatgaacca tatcaattgg
    11761 ttcttcttct tcgaaagtat ctacatagcg tgggatattt aagttatagt cattttcttt
    11821 gatttcatca aaggttgcca aatgagcgta tttctcgaca tcttttcttt cagcgtaggt
    11881 ttcgaggatt ttttgaatgt tttcttccga tagtttattt tgatttttcc ctttaacaaa
    11941 ttcacgacta gcatcaatga ataatacatc tcgagtttga cgattcttct tcaacacaat
    12001 caccgtcgtt ggaatcgatg tcccaaagaa caagttcgca ggcataccga tcacagcata
    12061 aatactgcca tcttctagta atttttgacg gatgacccct tctgcagcgc cacggaataa
    12121 aactccgtgt ggtaagacga tggccatggt acctgtttcc ttcaaatgat agaacccatg
    12181 aagaagaaac gcaaaatctg ctttggattt tggcgctaat ttcccataac gattaaaccg
    12241 agaatcatct aagaaagtcg tatctgctga ccaattagcc gaataaggtg ggttcattac
    12301 tactgcatca aatgtgtaag gttcatctgt tggccagtcc ttattcaacg tatccccatt
    12361 gcggagattc atttcttcgg cgtcgacacc atgtaaaatc aagttcattt ttgctagatt
    12421 gtatgtcgtt gtatttagtt cttgcccatg atatttaacg ttatctgggt gtgttaagta
    12481 gttacgtacg tttaacatca atgaacctga acccatcgtt ggatcaaaaa cactaaagaa
    12541 tcgtcgttcc ttttgatcga gcgttacgat ttgcgccatc atatcagaaa ccatatgagg
    12601 tgtgtaaaat tctccggctt tcttccctgc ttctgaagca aattgactga tcagaaactc
    12661 atacgcatcc ccgatcacgt ccccatcgtg ttctaaaaca tctacatcat tcaatttctt
    12721 gatgacttcg gtaatcgtca catttcgttg ttgctcatct gttcctagtt tttttgattg
    12781 gagatccaca tcatcaaata acccattaaa ttgattgtag gtagatgcca attgcacaaa
    12841 ggctttgttt agatcgttca attggaacgt tgcttgtttt gcttgatccg ctaagacgtt
    12901 gaaaagatac tctggtgcaa ttgcatatcc caaaatatca acgattgtgg caatcagatc
    12961 ttcttttgat tcttcatctg aaagtaattc acgatacaac attgtttgtt tgcttactgt
    13021 gtcatattcc tctaaggatt catctgctaa aagaaccact tgttccaaca atcgatcgga
    13081 taaatattta tagaaaatca gtcccaataa ataattttta tattctgacg catccatttt
    13141 actacggaga ttatctgccg cactaaataa tttttgattt aattctgcac ccaaaaagat
    13201 acactcctat ctcttattta aagttgctat ataccctctt attttagcat accccctttt
    13261 ataaaaagca aaaaaaaaaa gaaagaaact tattcttgct aataaactac gcgaaaggag
    13321 tctaacgttg tttgatataa cttagactga tcgttaactt attttttata tttctacata
    13381 gttatcttta gaaccatttc agatacatat gaaacaatta catatgattt tttcataaaa
    13441 aaacagtact gtctagtagt ataaaatata caaatttctt aaaaattttt tcactaaaat
    13501 cactacctga aacagtctaa aacacttata tatcaacgtt taatgaaact atctccgttt
    13561 catttcagat acaaataaat atagtcgggc tccaaatccg aaaaaaaata cccccagtag
    13621 cttatcctaa gctactggag atgtataagt acggtcatat aacaacgggg tagggtaaat
    13681 aaatgaaaaa gatggaagaa tcattattca ataacttttt taaatacttc gttgtaatca
    13741 gttacctgtc cgttcttaaa taccacagaa taatcttttt tcccattttc aagtaaccga
    13801 agtggaatgt ctttaccaac ttgctctgtc actgcattac tgatacctgt tagattagct
    13861 aataaatctg ataaagattg agcatctgta gaaatctctg tatcagtatt tatcgtgatt
    13921 cctttatctt catttacaag cttccaagta ggtggtatag ctgtgtatgt ttcgatgaat
    13981 gaatcaattc tttcatctat cgtcttccca gtatctactt ctgtctcatt actcttagat
    14041 gaatcgattc tttcatccat cgtctgctcc gtaactgctt ctgtctcatt attctcaccg
    14101 gaaactctct tcacacccaa acttatatat tgtccatctt tagtgtgcat agttaaaatt
    14161 cccacgcttt tactaataaa ttcactataa ccagttcttt caatagagac ttcaatgtat
    14221 ttcacactat catactcaaa agaaaaatag acatgtaaaa gcattttgtt gtttacatct
    14281 acataataat cctttgactc tacttgagac cttcctatac ggaatacagc tttactgtct
    14341 gatatctcta taccagcatc cttattatta atctgccaat ctccaagtag ctcttctggc
    14401 aagctagtag tttcttgtga acactttacc acttcactgg tagaggtcga aacattcacc
    14461 tccgcactgt tagaagaatt ttccaaggaa tcaggagcct tagcacaacc aacaagtctt
    14521 tatatattgt gcagccgata ttttcttcat tcagtacacc cttgaaatat gcagtgttag
    14581 ataaagcagt tcgtcctgat ttgctgaggc acccatctct tcttgaacgt actccttgat
    14641 tttcaaggcc gcccgatact ctttcggaaa acgctgcttc actgaagcca gcaaaaaatc
    14701 atccttctcc ggcaactctt ccttcttaat cattcgggca ataaaaaact gtaaatgcgt
    14761 cacaaatctt aagtaaagca tcgaatcttt atcaaccttt ttttcgggaa acagatatcc
    14821 tagcatagcc aagatatcct ttagtgctct taccgagagc atggtttctt ccatatttgc
    14881 ttgttcactt tgggcattta caatgtgaaa cgcaatgttt ccagcctcct cttctggcaa
    14941 ctgaacattc atttttttgt tgatcaattg gatagcatag cagccgatct caaattcttt
    15001 tgggtaatac ttttgaattt caatcagcag gcgattttga aaaacaatgc ctttttgact
    15061 gcgttcaatg gcatgcttca agtgatcaac caaggtaaaa aatagacttt tattcagctt
    15121 aaagtcaaac ttggcgttgg cataatcgat aattcgcttg acgcaaataa ttagatcatc
    15181 cggagcagcc tcaaacagtt taatgtaatc cttttttaga gcatcagaaa gtgaatctaa
    15241 tttaaatact ttttggattt tgtcagccgg aaccaaatcg cctttcttta gttgaaatcc
    15301 aatccctttc cccatcaaaa ttacttcttt gccttgctta tctaagcaaa gaattaaact
    15361 gttgtttaac gttttaatta ctctcattag attcaccgtt ctctctaagc taagccattt
    15421 cttgcgatta ctttttgata ccaaaagaag ctttcttttt ttattcgttt taattcttta
    15481 agattctcat cggttcggtc aacatatata aaaccataac gcttattgaa gccttgatgg
    15541 gtactaacaa catcaatcgc tgaccaaggt aaataaccaa aaacatctac accatctgtt
    15601 aaagctgctt gaatagccag aatatgtttg gctagataat caattcgatc ctggtcatta
    15661 attcgtctat cttcctcaac tatgtcgggt gccccatagc cattttcagt aattaaaata
    15721 ggtaaatcat agcgatcgta caatttcctc aatgttaatc gtagtccagt agggtcaact
    15781 acccagccat atggcgtttt atctacataa ggattttcag ctgcccggta gacaccttct
    15841 tctcccaaca tgatctgttg atcccccgct ctagcggaaa catctgatcc gtcatttttg
    15901 ctggcagcga tggtggcagt tgaataataa ttaatcgcaa taaaatcggg tttaccggag
    15961 gataaaattg cctgatcctc tgcctcaatt ttaggatata atccacgatc attcaaatag
    16021 ttttcaaaca aataattgta ttttccccga actgctacat ctaagaaaga ccagcctctc
    16081 aatgtttccc aattatgggc tgcaattgca tcttctggac tggaggtagc ttgatacata
    16141 gccgttaaat tgattgctgg accgattttt cctttaagat tcaactgatg atacaagtga
    16201 attactctag cttgagctaa taaaacatgg tgattaattt gaaagagttc tttttttgaa
    16261 ggcagctctc cacttggtgg caaaccaatc gctccaggat gaaggatcat cgtgttttgc
    16321 tcattaattg tcagccaata gttcaccttg tcaccatact cagtaagcaa tacctgtaca
    16381 tagtccacaa aataatcgat agttgctcga tttaaccaac cgccctgctc ctctagaaaa
    16441 taaggtaaat cgaaatggta catggtgaca atcggttcga ttccctgatt caggcattca
    16501 tcaattactt gatgataaaa agaaattccc ttttgattgg ttttattatt ttcaatatcc
    16561 ataattcgcg tccaagcgat ggaaaagcga aaagctttta agcctaattc tttaaacagc
    16621 tgtatatctt ctttatagtg atggtaaaaa tccgaggcaa cttcaaaatt agctacccct
    16681 tctgtatgct gatacttgtc aataatagac ggttgtttcc catcctcctg ataggcaccc
    16741 tctgcctggt aggcactaat ggaggcaccc cataaaaact tctctggaaa tgctgaaagc
    16801 tgtttgtatt gcacaaaaat cctctcctta actaatttgc atcaatggtt cgtgttcctt
    16861 taagcttccg cttgcttcac taattcctgc gtaattttca ctgttggtca ctaccacaat
    16921 cactgtatca tcgtagccag cgtttctaat ggcagcgaaa tcggcgttga ttaataattg
    16981 tcctgctgac acctgatctc ctgcttttac aaatacttca aagggttccc cttttaaatt
    17041 cacggtgtcg atccccacat ggatcaataa ttcaatacct tctttagaac gtagaccaat
    17101 cgcatgttta gattctaaaa tagtaatgac ttctccctca acaggtgaat aaatgctgtt
    17161 ttcttctgga tgaatggcta cgccctttcc caatagtccc ttagaaaaga cagcatcgtt
    17221 cacttcagaa ataggacaca cggttccttt taccggattt tcaacaatga ctgcttcggc
    17281 tattgccgtt tgctctgatt catcagaagt atcgtcaaat ccaattagat aagcaatgat
    17341 aaaggttgca acaaaggcga ttacacacgt aataattgcg tgaacaatat tcattggatt
    17401 ttctccaata aaggctggta aggctgctaa gccaggggaa acaaaggcat atctgactaa
    17461 accgctcaag ccagcataca aaccagcaat tgcaccacca atcatggaag caactaatgg
    17521 ttttttcagc ttcaaggtta ctccatagag tgaaggctca gtaattccca ttaaagcagt
    17581 gatcccagtg gacacagcta gctgcttcaa ctctttgttt ttagttttcg cggctacagc
    17641 aaaggtagca cctgattgag caatattaga agccagcatt cctggcccat tgatcatttc
    17701 agaacccgta ctgctcaatt gcatagtagc gattggcgtc attgcccaag ctgtacccgt
    17761 aaccaccaaa aatggctgca aaccacccat caaaaatgga attaaccaag ctgctttgcc
    17821 gttaatccat tcagccccca ttgctataag atcgttcagc aacgttccaa acggtccaat
    17881 gacaaccaac gctaaaggcc ccataattaa aaataccagc atcggcttag taaagaattt
    17941 aataaacgaa ggcgaaattc gatccgcaaa gggctctaca taggacatga tccaaacagt
    18001 aaagataatc ggtaaaactg atcccgcata atctgcagct aataccggaa ttcctagaaa
    18061 agaaacggca tcgccagtag aaaaaagctg tgaaatattt ggatgaagca aaatgccagc
    18121 aacagtaatc gccaaaattg ggttgacatc aaactttatg gctgccccat aggctaatag
    18181 tactggcata aaataaaagg ctgcatcagc aatagtattc aaaatgatgt aagtttgact
    18241 actgtctgat agtacaccag tcaaagtcaa gacggccaaa atggctttta ccatcccggc
    18301 acctgttata gctggaatca tcggtgtaaa ggtggtagaa atgacactaa tcaatcttga
    18361 aataatactt tcttgattgt ctgaagaggg gcccttttta attgaagttc ccacttcctt
    18421 ttgaatttcc ttgaaggctg tttggacttc attgccaatc accacttggt attgtccacc
    18481 gctatccacc acactaatta caccaagtgt tttttctaag gctttcgtat cggctttcct
    18541 tttatctttg agttcaaaac gcagtctggt ggcacaatgg gttaagctga ccacattttc
    18601 ttttccccca acctgttgca atacatttct tcctacttct ctgtaatcca ttattctctc
    18661 tccttaaagc agtaaaaaag accaagtcaa accacgcccc aatcaacatt gaagtgttag
    18721 tttgtcttgg tcttgcctgc ataatcagta acatgccaat aacgttattt tgctgtaatc
    18781 caataatagc acattatgat agcgcaatca acagggaatt taatttttta tcttttcgtt
    18841 tgaccgtata gggtaatata acagttagat cacatcaact agggtatatc tgcgtatact
    18901 tactgctaaa ataagtatat ctaatattaa ctataccagt aaaaccattc ctacataacc
    18961 agcgggaaat tcccaaaatc gtgaaagcat caaaaatgca tataacggat cacacaactt
    19021 caacaattct gctcttggga aaaattgatg aatttggtga gtaaaggatg gaataaacag
    19081 cattccaaca atataggcaa caatagtgat acttgttttt ttggtaaaaa tcagtaagca
    19141 atagccaatt tcaatcattc cgagatataa aggtatcgat agaatgattt gggctagtat
    19201 tcttaaaaat taactagcaa atgtttaaac tgatttattt ctaatctaca tgtagtaagt
    19261 atttcaattc caccaattat caataaaaat accaaacata tccataataa ttttttcttt
    19321 ttcatttctt ttatattgtt ataacagtga ataacataaa aattcccgac aatcattgta
    19381 atattttttt gtcacctgta ttagtattta atttatagga aactataaaa actccctagt
    19441 gaatttacac ctttgggagt tcctgtttgg acggtatgca taatacattt aaatcaattt
    19501 aacattaaca cctcagcata tggtatctcc acatatatac ggtctttctt tggatagtac
    19561 tcaatattta tgttgatagc acttccattg tctaatatag ctttagcccg gtatgtgtta
    19621 tctccatttt catgagtaat cgtcacatca tcacatgatc gatttaagtt gtactgttct
    19681 tgtaaaatct gtgttaccaa tggttttgat gacttctcta atccagatgt acctgtcatt
    19741 tttgaaaaaa taatacctat aatagccaag acaacaatac ctataatgac aaacttcttg
    19801 ttcatattta ctttattcaa ctttttatct cctatctcca aaataatttg ctcttatccc
    19861 tcttatcagt acattgacat ccctaaaagt acaccaaaga ttgctccagc tacccctgct
    19921 atcatcaaaa tcgtacccat tttctcatct ttttctcgat ataagtagcc cataataaca
    19981 gcaacagcac caaatacaac aggaataaat gctaacgata ctaccataga aatccaacca
    20041 ataatcaaat acagcttccc tgtctcttgt ccatgtacga tttgttcctt catttcatta
    20101 attgtctgct cagtactttt ctttactgtg tctgttccgg ttgtctcatc tgtttcttgt
    20161 agtgctccac aaaactggca atacttactt tcatctgtta tttctttgcc acattttcta
    20221 caatacatgt cttctcctcc taaaatcatt caattgctta ttagttaact gtttacttta
    20281 ttaaatacat cagaaaacag ctcattcatt ggtacactta atatctcaga aagcatcttc
    20341 atttgatagg gtttaggaat cgttctccct gtttgccacg aacttacaca ttgctgtgta
    20401 acattcattt tttttgctaa ctccgtttga gaaagtcctt tttcttttct gtatcggctg
    20461 agattactta cttttttcat gattctcccc tcctgtttta ttatatacaa caatatgatg
    20521 tatttcaact cataaaatat aaaaaacttc aaataccatt acaaatacaa caacttgttg
    20581 tactatactg agatgggggt gtattaatga aacagaaaca atatgcaaag gtcattagag
    20641 aaaagcgaaa agctctcggg tggacacaaa aagaattagc tgaaaaaatc ttctctacac
    20701 aacaggcagt agcaagatgg gaaaattctg taacagagcc taacctcgat agcttaacag
    20761 ctctttctag agccctagga acccctgtaa gccattttct agacaatgtt gtcgtagatt
    20821 atgaggaaga gtttttggct ctctatcgtt ctcttagcac tgaagatgca gttcgaacaa
    20881 ttgactatat gaagctatta aaaaggcaag aaaatgaacg caatcagcta ttgaaagatt
    20941 gaaaaataaa taacccaccg aactttaacg gtgggtgtat tgagatgaaa taagattaag
    21001 agctatggat aacctgtcta gtttaataca attgggtctt ttctcttcct tactctccgt
    21061 ccatcatctc ttgttacact tgtaaattga tacagaggaa ctatcccagc tcctgataat
    21121 atcaattctt ttattgtttc atctatccct gcccctttcg ctaaatatat taaaaaacta
    21181 ctacttgctt tcacattgta tagtttctta atatccttag aagctcgttc aagagtgaat
    21241 ccttgtttaa caaagtagcc tctgtttttt attaaagcat ttttcacgct gttatcccca
    21301 acattacgta ggcgttcaca aacagtgtta ataagttctt tctctgtcat tagatatgtc
    21361 ccttgccacg ataagccctg taaaacttca tgtgcttcat ctaaaatcaa ttgtttttcc
    21421 ttgctgagtt ctgatcggga attttgagga aatactcttt tagcctcttt ttcgttaaaa
    21481 catatcttaa cccattcata agaaaaatat tgatatgtaa aatgaatttt ttccagaagt
    21541 ggaaggatat cttcattcaa tttttgaatc acagaagttg cattaaaaac gtctaatcga
    21601 tagacattgc ttctcttatg attaaagttt cctaacgctt tctcttcctt taacctattt
    21661 aggtattcga gcatcttcaa aggaacatct tcatcatcta atttttttat caatcctata
    21721 aaagtcataa gtgatagtaa cttattacat ttatctatgt cataagtttt accatagtaa
    21781 gaactcattt ttttagctaa ttgttccccc gacaaaatac aagtcacact ttccttacta
    21841 tcatcaaaaa aattaacatc agaaagcaag acatcataga cacgtcgcat ttcctctaga
    21901 taattcctta ttgtcctgaa aagtttgtct ttcattgatt caagagtgtc aaaaagtact
    21961 tttgattcct ctttcaattt tcgaaaatgt gtaggataca agtctaggtt ctctaatagc
    22021 caattaaatg tttctacccg actgtctttc tttaaaatta gcatcacaac tccaataaaa
    22081 ttttcactga tatcacaatt actggaatga caataaaaaa tggaatgccc agttttactg
    22141 gtaaaaatac tagcagaggg attatgatcg ttgtgaaaca gacatcttaa gtttttactt
    22201 gacttaagta atttattcat atcaatggtt aacaatcttt cataaatctc attaaaagac
    22261 atgtcggagg aatcaaacag gcagtcttta aaccactgtt tcatttcttc tttgtcgtca
    22321 ttttttatca acttaacaaa atcggtttca ttgactagtt catactctct ccctgaatca
    22381 ctttgacaat ttttagaaca tgtcatggta aaatcaatcg gaagctcagt aatatctaac
    22441 atgttgtctt cattaattat tatcactgca tctgacttat atcctccaaa aaacattcta
    22501 ttcgaagcat ttacattgtc atcacacccg gggatgttct ttttcaagta ttcgtaaaca
    22561 gcaaccattt taccatagtc agtaattgct tcttttaata gaaagacaat cttgtatttt
    22621 tctagatttt cagagtaccg aatacttttt tgtatcgcaa acgcagaatt atgaactaaa
    22681 ttgatagata aaaccttttc tacgcttaag tatctactat catctttact caaatctact
    22741 accttcccct tctcatcttt aacagtgtta tcgacatcca gaataattac tctggtttcc
    22801 acaaaactct ccttgtcatt gcctttattc tttgtcttgc ttggaataaa tgattgtgat
    22861 gaatttagta tctctacaaa ctcatctctt gtaacttctc tatagttatc taaacaccta
    22921 tctcttacat cagttttttc tttttttgta actggcttct ctaataattt ttcttgatct
    22981 acataaattg gaatattaat cattgatgtt tccatttgtg tttcctcctt ataaaattct
    23041 gttaaagact aaaacataca gataataaaa acaaactctt tattactata tgttttagtc
    23101 ttatttgttt actcaacctt tataccgttc catgaaacta tcaatatcag attttttaaa
    23161 tctaatgacc gtatcaattt ttataacagg taacccgtat tgagaaacat attttctaat
    23221 cgttcctact gatacaccta gatattcact tgcctgctga tagtccatat actctagagt
    23281 gactccagaa ataatgtctt ctagcatagg ctttagcact gtaattatct ctttcacttg
    23341 ttgttcaact tgtatagaaa caatgctatc aatgatttgt agagacatat ttccccctcc
    23401 tttcgtgatt gcttgaacaa attttctaag aattatcttc gtattttttg ctcaaaataa
    23461 aagtattttg ttattaaccg attaatcaaa caaaaaatga ttaccagttc atcgacatac
    23521 acataatacc tcgataaaaa aggggtgtca acagctttgt aaaatttatt tgcaacaccc
    23581 cttttttgag gtatactaat tttaggaggt gtggtcaata tgataaaacg tccaccaata
    23641 aattatttag aacgaaaaaa gatactcgga acaaaaataa aagctataag aaagagtaaa
    23701 aaattaacac aaccagcatt tggtcttatg attaacaacg gtcaattaat tgacaaaaaa
    23761 actatctacg aatgggagaa gggaacctat ttaccaatac cagagcgatt atcccgaatt
    23821 gctgatttgg gaaatatgag tatagaggag ttagtttgtg gcaatgtaga agaatatatt
    23881 ttgggaatta ttctttatag agatagcatt gtactagatg gtataacatt tccagataaa
    23941 aacctgttcc agcatcttcg tcaacagttc ccaccagtac actctaattt agatacatgg
    24001 ctggatagat attccaaatt agaaccagag atgcaagaat ttattgctaa caagacttgt
    24061 aataaagtta aaaatgaaaa aattagtctt ttcaatatct taaaaataga agaattattc
    24121 atcaatgcta ttgtagaaga atttgacaat aacattcttt tcctcactag cagtatagaa
    24181 gaactactag aaagaatggt agatgaatgg ctacctattc aattgaaaga catgtcttat
    24241 cccgaagaag ctgtgagaga aattactgat aatataaaca agctagaaca aacgatttca
    24301 tcaattggga aaaaatacac taagaaaaaa atgaagggag gtgataccat ctagtggtaa
    24361 gtactccttg taaactaaga ggagatgatt taatggcaaa aatatcaaag tacaaaaaga
    24421 aaaacggtca agtggcgtgg atgtataaag gacacattgc caccgatcca cgaactggtg
    24481 aaaaaataaa tactacgaga agaggtttta gtagcaaaca agaagctcaa aaagattatg
    24541 acgagtacag acatcaaata ttatacggtg ttaaaaagaa agcacctgat atgacatttg
    24601 aagacctgta taatgaatgg ataactcatc agcgtaccag tgttaaagct tcaacaatcg
    24661 caatctcagt aagatacgca aataatcaaa tacttccagc atttggtaag cttaaactat
    24721 cgaatatctc tgtcccatat tgtcaaaaag tggtagatga atggcacagt aaatatgaaa
    24781 gttacgatta tatgagaaaa cagactgcac aaattttacg ctacggggtt gcaatgcaat
    24841 atattgacaa taatcctatg gaaaaaacac ttcttccacg aaaaaaagag tatgagaaaa
    24901 atcgtaaatt ttactcaaaa gaggagctta ataatttact ggatgctttt aaagattttg
    24961 gtaacatgaa acaatacgcc ttcttcagat tattggcata cacaggtatg agaaagagtg
    25021 aagtactcgc tttacagtgg aaagacatag acacgtttaa taaagaacta catgtcaata
    25081 aaactcttgc tgtagatgaa tttggtaaag ttataattca atctccgaaa acaagagctt
    25141 ctagacgagt tatctcttta gatactgaga ctctttctat cctcaacaac tggaaattac
    25201 agcaaaaaga agagtattta aaacttggat acaatactag ctctaaagag caacatgttt
    25261 ttacaacagt taaaaacact ctatacattc caaacactgt taatgattgg ttaagatata
    25321 ttttgaaaaa atataacttg cctcgtatta cacctcatgg gtttagacat acacatgcta
    25381 gtttattatt agaagcagga gaatctgtca aggtagttca acaacgatta gggcatgaaa
    25441 atagtaaagt gacattagac atctatgcac atattacaaa taatgcacct aagaaaacag
    25501 gtcaagactt tgctgatatg atggctcatc agtaaaagag ctggtcaaaa tctggtcatt
    25561 tctagtatac aggcaaaaca aaaaagctag aacccttata tatcaagggt tctagctcct
    25621 ataacaggtt tacccaacac tgccttccat ctcataactg atcaatcgat tgagttcgac
    25681 tgcatattcc attggaagtt ctttggtgaa tggttcaaca aagcccatga cgatcatttc
    25741 agttgcttca gattcggtca ggcctcggct catcagatag tagagttgtt cttctgagat
    25801 ttttgagact ttggcttcgt gttctaaaga gacttggctg ttgtggatct cattgaatgg
    25861 gatcgtatcg gatttcgacc attcgtccat gatgatcgtg tcacattcga tatgagagat
    25921 cgaacccgca ctgtttttgg caaaggttac ttgtcctcga tagtttactt ctccgccgtc
    25981 ttttgaaatt gatttagaga cgatagagct agatgtattt ggtgcattgt ggatcatttt
    26041 tgcacccgta tcttggattt ggtttgctcc tgcaaaggcg atggagagca tggtgcctcg
    26101 tgcacctttt ccatctaagt aaacacttgg gtatttcatc gttgtttttg cacctaggtt
    26161 tccgtcgatc cattcaacgg ttgcgccttc gtatgctttt gctcgtttgg ttactaagtt
    26221 gtacacattg tctgaccagt tttggatcgt tgtgtaacgg caatacgcat cttttcttgt
    26281 aaagatttct acgatggcgg catgcaggct gttacttgaa taagtaggtg ccgtacaacc
    26341 ttcgacatag tggatgcttg ctccttcgtc aacaatgatc agcgtacgtt cgaattgccc
    26401 cgtgttttcc gcattgatcc ggaagtacgt ttgtaatgga acatcgactt tgacgccttt
    26461 tggcacgtaa ataaaggtcc cacctgacca tacggctgaa ttcagcgctg ctaatttgtt
    26521 gtcggttggc ggtacaagtt ttgaaaagta ctctttgaat aattcagggt attctttcaa
    26581 agcagagtca gtgtcagtaa agacgattcc taacttttcg aattcatctt tcatattatg
    26641 gtacacgact tctgattcat attgtgcgga agcaccggct agataagctc gttcagcttc
    26701 tggtatacca atcttttcaa atgtatcttt gattttttct ggtacgtctt cccagtcacg
    26761 agcgggtttg tcacttggtt tttggtaata tttgatggca tcgaaatcga tgtctgaaag
    26821 gtcggggccc catgtttgca ttggcatttt gttaaatgtt tctagagatt tcagacggaa
    26881 ttcaagcatc cattctggtt cgcctttgat tcttgacatc tcacggatca cttcttctgt
    26941 caagccttct cctgtactga aaaccggttc gacatcatcg tgaaacccga atttatattc
    27001 ttctaattct ggtacgccca tgttctcact cctcttcctt gtggtaatga tggaaagctg
    27061 cggtaccatc gttttccatt gcttttccta gtgctttcca ggcaagtgtc gcacatttga
    27121 tccgagcggg aaatttagct acaccaccaa gcatagcagc atcgcctaat tggtcgggat
    27181 ctttgacttc ttttccttgg acaagctgta gaaagtctac agttaaatgt tctgcttctt
    27241 ctattgattt tccgatgacg gcgtctgtca tcatcgaggc acttgccgta ctgatggagc
    27301 agccactgcc gtcaaaggcg atatttttga tgattccgtc ttttacatcg acttgcaatt
    27361 cgatcacatc tccacatgtg gggttgttca attcgatctt gcttgtcgag tcagtcaagc
    27421 ttccacgatg atgcggatgg gaagaatgat ccaagatcac ttgacggtat aaattgtcta
    27481 atttagatag tcccatgttg gaaaaactcc tttgttgcca tgattgcttc aactaaacgg
    27541 tcggcgtctt gcgttgtgtt gtagaaataa aagctcgcgc gagcagtcgc tgatacttca
    27601 aggtacttca atagcggttg cgcacaatga tgacctgctc tgacggctac cccttccatg
    27661 tctaaagctg tcgcgacatc atggggatgt aacccatcca aattgaatgc aacgactcct
    27721 gtccgaaaat tcgggtcttg tggaccataa acagtcaatc catcaattgc ttgtaatttt
    27781 ggcaacacat aagcggctag ttcttcttca tatcgatgga tattgtccat accgagtttt
    27841 tgcaaataat cgattgcatg gcctaatgcg atagcgccgg cgatatttgg tgtccctgct
    27901 tcgaatttcc aaggtagttc tttccatgta ctgtcgtata attcgacaaa atcgatcatt
    27961 tcgccaccaa attcaacagg ttccatttct tctaaccatt ttcgtttacc atataaaaca
    28021 ccaatacctg ttggtccgca cattttgtgt ccgctgaaag caaagaaatc acagtctagt
    28081 gcttggacat caatggccat gtgcggggca gattgagcac catctactac gatgaccccg
    28141 ccgttttcat ggacaagttc agctaattct tgaacaggat tgaagacacc taagacattg
    28201 gatacatgag caatggagac gattttggtt ttgtccgtaa tctttttcgc tgcatcctcc
    28261 atatccagga agccttcttc tgttagttcg atatatttca agacagcacc tgttcgtttt
    28321 gccaactgct gccacgggat aacattagaa tgatgttcca tgtaagagag cactatctcg
    28381 tctcctgctt tgatatagcg gtcgccgaag gttctggcta cccaattcaa gcttgtcgtc
    28441 gtaccacgag taaacagcac ttctgctgtt tcttttgcat ggatgaacgc ccgtactttt
    28501 tctcttgctt gttcataatc atgggtcgcg cgttctgcta acgtatggac accgcgatga
    28561 acattggcgt tatcgtgttc gtaatagtac gctaatgcct ggagtacttg ttttggcttt
    28621 tgggtcgtcg ctgcattatc cagataaact aaaggttcat cattgacgtc ttgaaataag
    28681 ataggaaaat cattgcgtag ctttttagca tcgatcatcc attcaacttc ccttcgatta
    28741 cttcgactaa ttctttccga acttgttcta cagggattgc tgttaagaca gaaccgagga
    28801 atcctcgaat cactaagcgc tctgcttctt ctttatgcaa tcctctactc atcagatagt
    28861 acatttcttc tggatcaact cgtcctacac tggcagcatg tccagcagtt acttcgtttt
    28921 catcgatcaa aagaatagga ttcgcatcac cacgtgcttt atcagaaagc atcaagacac
    28981 gactttcttg ttgggcatct gcaccttttg cccctttcaa gatgtggccg ataccattaa
    29041 aggtcaatgt tcctttttct cggatcactc cgtgttgcaa aatatgcccg atcgtatgag
    29101 gggctttatt ggttactcgt gtatcgattc cttggatttg tctgcccgag ctgattgcca
    29161 cgattttgac ttctgcgtgt gcaccttctc ctgctaaatc tgagtcaaag tcagcgatca
    29221 catgaccgtc attcatgaca ccaattgccc agtcgacaga agcatctcgt aaaatgtgcc
    29281 cacggcgatt catataagaa gtaatatttt ctcctaattg atctacggct gaatatttga
    29341 tttttgaacc agctttagcg atgacttcca cgatgatatt acctgatgat tttgctactt
    29401 gttctttggt tgtctggaaa cgttccagat aagaaaattc actatgttca tctgccacga
    29461 tcaacacatg cttgaagaag tgctcatcgg atgcgccatc ttgaatgaat aaggactcga
    29521 gtggttcatc gatcacgaca tttttaggca catacaggaa aataccgctg ttcataaaag
    29581 ctacatggag tgcagtcaat tggtcttcgt tcatgtttac tgcttttttc atataatatt
    29641 cttggactag ttctggatat tcgatcattg cagtgaacaa gtcggtaaaa atgactcctt
    29701 tttctgctaa ttccacaggc agttgttcaa aaatcgtcca tgaaccttgt tggacaagca
    29761 caggattgtc tttcattgca tcaaatgccg gaatcgtgcc tgtttcactg atggtttgtg
    29821 tttctttcac atcaaataac gaccagcgat ggaatttcac ccgatcgata tgcggcaatg
    29881 gcagttcatc tgctttcgct agtgcgtctt gacggaaggt tctcatccaa tcaggttcgc
    29941 ctttacttaa agaaaaatca tttaccgcgt caagataatc gagccagttt ttttctttca
    30001 ttcttgctcc cccttacgct tcttcttctt tatagtcgat tcctaattct ttactgatac
    30061 cagcgtagcc ttctgcttcc agacgtttag ctaggtcagc attccctgtc atgactactc
    30121 ggccttccat catgatgtga acgacatcag gtgtgatata gttcaataat cgttgatagt
    30181 gggtgatgat caatgcaccg aaattgtctc cgcgcatttc atttactcct ttagcaacga
    30241 ctttcaaagc atcgatatcc agacctgaat cgatttcgtc taaaattgca aatgtaggtt
    30301 ctaacatcaa caattgcagg atctcattcc gtttcttttc tccgccagaa aaaccttcgt
    30361 tcagataacg ttcagccatt tcttctggca tatttagtaa agccattttt tcatctaact
    30421 ttttcaaaaa tgccataaca gaaattttgt cgttttcatc acgtttcgca ttgatcgctg
    30481 cacgcatgaa ttccgcattc gtgatacctg gtatctcact tggatattgc atagctaaaa
    30541 ataagccaag tcgcgcacgt tcatctactt ccaagtctaa aacatttttc ccgtcaaaca
    30601 aaatttcgcc ttctgttact tcatagtttg gattgcccat gatagcggca gataaggtag
    30661 attttcctgt gccgttaggt cccatgatgg catggatttc tccggttttc attgttaggt
    30721 ttacgccttt aaggatttct ttttcctcga tcgacacatg tagatttttt atttctaaga
    30781 cagacatgca ttataccccc aaattctctt tcgttctcac tcattttaga ctaattgaga
    30841 atgaagcgca atgaccttcc ttactaaaat caaatatctt ttatttatta ttaaacaatt
    30901 ttgatttcta aattagaata ggtataaaaa agatgagaaa taagccatta cagacttatt
    30961 tctcatcttt tttgttccat accttatttt gcatcagcct ttactggttt aacagagccg
    31021 ccccatttgt ctgtgatcca ttcttggact tctttgcggt gaagcacttc aatcagtttt
    31081 ttgattttgt cactgtcttc atcaccttta cgagtcgcaa taatattcac gtaaggagag
    31141 ttgtcctttt caagcaatac ggcatcttct aatggattca accctgcgcc gtaagcgaag
    31201 tttgcgttga ttgcaactag gtcaccttca tcattttggt acgtagaagc taaaagtgat
    31261 gggtcgatcg tatggttgaa cttcaagttc ttagggtttt cttcaatatc atcgaatgtt
    31321 gctgtttcca gatctactcc atctttgact ttgactaagc cagcatcttg tagaattgtg
    31381 atgatgcgtc cccagtctga ttcagaattt gaagtaatga ttgttgcacc atcttttaat
    31441 tcggaaacat ctttgacttt tttagagtac aagcccattg gttcgatgtg gacagcaccg
    31501 gcattgacaa agtcatatcc tttttctttg atttctttgt tcaaataagg aatatgctgg
    31561 aagtaattgg catcaatgtc gccttcttcc aaagattggt tcggtaatac atagtcatcg
    31621 aatttgacga tttcaagatc gattccttca tctttcaata atggtttcac atgttccaaa
    31681 atctctgcgt gcggtgtcgg tgaagcgccg actttcaatg tcgtgtcgtc tgctttattt
    31741 gctgaatctt tgctgtcgct gttgttaccg catgctgcta atcctacagt cagcaaaagc
    31801 gctgctgcaa atccaaaaat tttctttttc atatcctaca tccccctata tcctattatc
    31861 ttttgtctgt tttcttagtt aagaaatcac cgattccttg aacgataaat acgattagta
    31921 aaataattaa agtcgcaacg aatgtcactg ttagattacc acgctggtag ccttcctgcc
    31981 aagcaagacc tccaagtcca cctgcgccga tcgcgccggc catcgcagta aatccgatca
    32041 tcgagatcgt tgtaactgtt aatccagaaa tcaatgcggg aatactttca ggtatcaaga
    32101 ccttccagat gatttcccag taacttgctc ccattgcatc tgctgcttcc agtacaccag
    32161 ggctgacttc acgaaaagca atctctacta gacgggcata aaatggtgct gcggataaaa
    32221 caagagctgg aatggctgct tgcgcaccta gcatagtgcc tacgattgct ttagtaaatg
    32281 gaatgatcaa aaccatcagg atcataaatg gcgttgaccg gaaaatatta ctgatgatcg
    32341 atacgattcc ataaatgatt cgagcatacg gtttgttttt gcgtccaata gagtaaagga
    32401 tcaatcccaa aactaatccg atgatcgtca ctaagaccat tgagattaat gtcatatata
    32461 gtgtatcaag gatagcctga ttcatcgttt ccgggttgat ttgtttaaaa tctagatatg
    32521 tttctgaaaa gcttttatcc acgatggatc acctcaattc ctactccttg ttctttgatg
    32581 aacacttctg cttgatctaa ttctttggtt tcaccagtca tcaagacagt caatgaacca
    32641 aaggaacctt ctttcacttg tttgatcttt ccatacacaa cgttgacatc gatgttatag
    32701 cgtcgaatcg cttgggaaat gactggctcg ttggcatttc tttcgctaaa ggtcaaagtt
    32761 gccaaacggc cgcttggatt ttctttgatg agttcagcta atagttctgt cgaatcagct
    32821 tctggttcga tgtcttgttg tacaaagcgt ttggttactt cttttttcgg ttgtctgaag
    32881 acagtcaata cgtctccttc ttctacgact ttccccaact ccatcacagc gactttgttg
    32941 cagattttgc ggatcacatt catttcatgt gtgatcaaga cgatggtcaa attcaatttc
    33001 ttattgatcg ctaacaataa gtctaatact tcttctgttg tttgcgggtc caatgcgcta
    33061 gtagcctcat cacaaagcag gatctcagga tcatttgcca atgctcgtgc gataccgacg
    33121 cgttgtttct gtccgccgga aagttgtgaa ggatatgctt gtccgcgtcc ttctaacccg
    33181 actaaacgca acagttcttc tgcccgttct ttacgtttgc tctttggtac gccagctaat
    33241 tccagcggca gctggatatt ttcaagtaca gttcttgacc ataacaggtt gaaatgctgg
    33301 aagatcatac cgattttctt tcggaatgta cgtaattctt tgttttttaa ttttgtaata
    33361 tcttgatcgt ttacgatcac ttcgccgctc gtgggaagct caagtccatt caggagacgg
    33421 actaatgtac tttttcctgc gccagaatag ccgacgattc cataaatatc ccctttttca
    33481 acgtttagtg aaacgtcatt gacggcttgg atgtttccgt ttttcccatt gaatgtcttt
    33541 ttgacattcg ttagttggat taatgacatg ccattcacat ctttctctta tgatttttca
    33601 aacaaaaaag ccttcgccct cttgccttaa gcaaaaggac gaaagcttga acttccgtgt
    33661 taccaccttg atttaccgtt acttcgcaat aacagcctct tccagtactt ggacttagct
    33721 tctttccgta tactgtcgcg ctgtaacggg cactcccgta gtaagtttgc ttacgctcgc
    33781 ttactctgct ctaagaccat cttccatctc attttcgtta cccattttca gcaaacaggg
    33841 ctctcttcaa acgtccgtga catgtactct tcttttcaaa gctttctcgt ttcatatagg
    33901 attgcttatg attatagcaa ctggaagaat cagtgtcaac ttttttcaag tgattttctg
    33961 attttataat tcgtcaaatt cttttggatc aacatctttg aaacttaaga tccacgcatt
    34021 catttcatct gaatcattca agatattgat atcttcaccg attttatcat taacagaaga
    34081 aactgtaccc gttagcgggc tggcaaattc attgaccgct ttttccgctt caacttcgat
    34141 cagaggttca tcttttttta gcgattgtcc tgcttttggc agattggcaa acgtgatgtc
    34201 tccaagttct tcttgcgctt catttgtcaa accaacacag tattcttttc cattaaataa
    34261 gatccaaagg ttgtcctttt tcttcaaatg cttgcctgcc attttatatc agttctcctt
    34321 cataaatttt ttcattgaat ccaattgcaa caagctggtc ctctcgtaag atcagtggtc
    34381 gcttgatcag cataccatct gtcgataaga cttggctcgc ttttgaagcc gtaaactcgc
    34441 ctacttgatc tttcagtcca agttcccgat atttcatccc gcttgtgttg aacaggcgac
    34501 ggattggtac ttgactcgtt tctagccaag tctcaatcgt tgaggccttc ggtacttctt
    34561 tgatcatatc aacggtttga taactaacgc catgctggtc taaccacgct ttggcttttt
    34621 tgcatgttga acatttcggg taccagtaaa aggtgtacaa ttttcttacc tccttgaaga
    34681 tcttgcaggt gcttcatttt ggtatctcat cgataatatc aacccgatac cgatcatgtt
    34741 ccctaaaatc gaggatcctc cctgactaat gaaaggcaat gggatccctg ttaaaggaag
    34801 taatccgata tttgctccaa tattctcaaa gacatggaac agcagcatca tgatcaaacc
    34861 gcttgcgata taggcgtaaa attcattatt cgtatcgaaa cacacccgaa tcatacgata
    34921 gattaaaata aaatacaaga aaatgacgaa tgcaccaccg ataaagccga agttttcacc
    34981 gataacggta aaaatcatat cggactcacg aacaggcaca tagatattgc ttacgttgaa
    35041 tcctttacca aacaagccac cggtaccaat tgccaaaagt ccttgggccg gctgataact
    35101 cattcctgac gtatcatgga aagggtccag ccatgaatca atacgtgcaa actgataaga
    35161 tttgaatcca actttataaa gcaagtctct tccgccttca gtcgtaacca agaagatcgt
    35221 tcctgctcca acgactactg ccaagattac tacaggtgcg atgatgcgcc aagagattcc
    35281 agacatcagg aaaatcccgc caaagatcgc aagaaagaca agcatcgttc caaagtcatc
    35341 ttgggccatg atcaatgcta aaaccggtat tgtcacgatc aagagcttcc caatcagcca
    35401 aaagtccgat ttcatcgtcc gttcttttac ttgcgtatta tgtttagtta cgatcagtgc
    35461 catcatcaag atgtacgcaa tcttcatcaa ctctgctggc tggaaagtaa acgcaccgaa
    35521 tcgaaaccag tttttagacc ctgttgaagc agctaaattc tgatcataga atttgagcaa
    35581 gagtcccatt accactaacc ccaaaccata aaggtaagga gtcagtttcc agatccattt
    35641 ggaattcaaa tgcatgatga caacgattgc tgccgctcca actaaatacc agacaacttg
    35701 catccctacg cctttcaaga ctgctggatt gttcggatca tggctaagtg ctacatataa
    35761 tgagagcaga ccaatcaaac ataacagaaa gactggtaaa atcacgccat aatcaatacg
    35821 attatcgttc gaagctttta attttttttc catgtttcat cccttttctt aaaaaccata
    35881 ctgctgatta ttatagcggt ccagttagat aatggaaagt ctttccatag acatttggga
    35941 aaaattaact ttccgtttgt gcagttgctt gagcaaatgc agtacaaaag cctactgctt
    36001 atctttagtg tatcatagaa ctttttttga tacatgcaat tatctaccag ctgagattct
    36061 cctcgctgtg agaagtttct gtgctttcat tgtatcgaag gatctcttcg gcaatctcta
    36121 gcagctgatc agaaaccaga ttggttcgtt ggatgaaata aaaatatcga tggaaattca
    36181 acggagtcat tggcacattt tctggaacag agcgcttaga caaaatcgag cgaccgattc
    36241 cttccttcag caacgcaaca attacatcgt tacttttcac gatcatcttc ttatcttgga
    36301 tattcatctc ttcaaaataa cgctgcgtgt aatgaaaaac accagagttt gcttctctta
    36361 caagccacaa tttgcttttt gggtccccag cgatgactag ctggtcttcc atcaaagagt
    36421 atctttgtac gccggaagtc tctaatggct tttcgataaa accaaaatcg gattcatgtt
    36481 tcgctacttg ttctaatgat tcatgagaat tcgtgatttc gatctcaaaa tgcactttag
    36541 gaaacctttt gatcagatgt ttcatcaact gaggcaaaag atagacagca aaggtgtggg
    36601 aagcaacgat ccgacatact tcttcttcat tttgttcatt gagcaatttt tgctgagttt
    36661 cttcccattc ttctaacaaa accagtgtcc gttcatataa taagtcggct tgcttagttg
    36721 gaagcatctc ttttcttccg ttacgttgaa aaagatcgat attcaattcc cgctccaact
    36781 gtttgatttg attcgagact gctggttgag ataaaaacag tacttctgct gctctggaaa
    36841 aatttcttgt ttcatatact gctcgaaaag tcgttaataa cttaaacatg gcaccacttc
    36901 cataactttt tatgataatt actataaaaa ctatttgatt taaatataca aaaaaccatc
    36961 tataatagaa atgtacaaca aaacagttgc tgatccaact agtaacttag atgaagacaa
    37021 tcagctgtct gcttgctcgt aatcatacat tcctattcat tttacaatgt attcgttcat
    37081 cttggtacta aaatctatta aataaaaagg agaatattat atatgaagaa ctttttgaaa
    37141 attattccga tcccgatctg cgggttgatc cttgggttag cttcccttgg aaatctgttt
    37201 aaagactatc accatgttgc tttaggcaat ctaactggcg gtatctctat gattcttatg
    37261 atattcattt ttgttaaatt gtttatctta ttcgaacaca cgaaacaaac gttgcatgat
    37321 ccaatcatcg cctccgtttc accaactttt acgatgtcac tcatggttat ttgtacttat
    37381 tttgtttcat atgaaagtat tgcaccaatt gtcaaattca tttggctgtg cgctgttatc
    37441 ttccaagtga tccttgtctt ttatttcaat tatcaccatg ttatcaaagc tgatctttca
    37501 attgaagcaa tttatccaag ctggtttatc atttacgttg gatttggggt catcactgta
    37561 actgccggga acttcttccc acttattgga aaaattttct tttggatttc attgatctgc
    37621 tatgcgttct tattgccgat catcatcaaa cgtgtctttt tcgtaagaaa tatggctcat
    37681 ccgacacgtc cgctgattac gatcgttgca gcacctggtt ctctttgcct aacaggttat
    37741 cttaaaaact ttgctgaacc taatttttat cttgtcgtag cattgttcgc tttatctcaa
    37801 gtattgtatt tcgttgtgct gtctatgctt ccacgtttgt tcaagcttcg tttttatcca
    37861 agctatgcag cctttacatt cccgcttgtt atctctgcaa cagctatatt tactactgtt
    37921 cattacttca acagtttagg tatctatagt agcgtattgg atttcttaaa agttgcggaa
    37981 tgtctgtttg caagtgtagt tgtcttctac gttcttgctc attacgtacg tttcttaatt
    38041 aaagaacata gcaaacatgg aaaatttgtt tctaaaaaaa tcgcttaata atcgattcaa
    38101 taggttgtga aaaagctgtt ctgcatcaag taataagaac ggccaaacaa aaacagctgt
    38161 tcatgttttt gtactggttg ggcttattac cgcagatgca agcttttgaa caccgtttac
    38221 caagaggtcg taaaagaagt ggtcagcttc aagaaataag aaggaattta cgaaaattgc
    38281 ttctcaaatt tttgtgaaat ttcggcttat ttccgtagaa gctacttctg gaacaccgtt
    38341 tattcgaaac aagaggtcgt acaagacttt tgtcacgacc tcttgttctt ttgtcaaaat
    38401 actggtttag tgctactatg ttcgagtatg cttttagtat ttattgacga aaggaaccga
    38461 tcaaatgaaa tcattgtcac atccaataaa atctgtaaag gattcagcgg tccgcgaaat
    38521 cttacccggt ttactgcttt cttttgccat cgctgccttc agcaggcttc ttgctattta
    38581 tctgccacag ttaggtggag cgactattgc tattttattg ggtattttat taggaaatac
    38641 tttttttaaa cagcctgttc tagcaatcgg cacaaaattt gctgaaagca aactattaga
    38701 attctctgtt tttttactag gtgcaactgt cacagctcaa acgattgctc aaattggtgt
    38761 aaaaggaatt gtttttgtgt tactgcaaat gtcagtcact atcatcggtg cttacttgat
    38821 tggtaaaaaa ctgcaattca gcgatacttc ttctttgatg atggctggag gaaacgctgt
    38881 ttgtggttct tctgctattg cttcaatcgc cccttccatc catgcgaaag aggaagaaaa
    38941 aggacagatc atcacactag tcaatctact gggtactgtg atgatgttga ctctcccttt
    39001 tcttggatta cagttgtttg gtggaaatct cattgaaaaa agtgcacttt tgggtggtac
    39061 actccaatct gttggtcaag tagtcgctgg ggcaagctta cttgacaatt ctgttgtcga
    39121 attcgccatg ctatttaaga ttctacgtat tatgatgctc gttgttgttg tctatctgtt
    39181 tgaaaaattc atgcacaaga aacaaccagc tctgtttgaa acgccaactg atacgcagaa
    39241 aaaacgaaaa ttaccgcttc cttggtatgt attagggttt gtgatctttt gtttggtcaa
    39301 cagcttgttt tctttacctg tagttattga ccaatcggca cattttatca gcagctggtt
    39361 tgagatcact gctttagcgg caattggtct gcgtttagac tttgcaaaat tcatgaaaga
    39421 aggtacacgc ttcttatctt atggattagc tgtcgggctt ctccaaacag tgatggcttt
    39481 actatttatt tggctgatca aagtttagaa tgaacatgaa aaaacagttg tgattctcgc
    39541 atggaacaat gtccgaatca caactgtttt taattttgca gatatccttc taacaacaac
    39601 aattggcgtt tgatttcaac cccatctatt gcgtttccca tatatcctgt cagttttcca
    39661 ttttgcccga tcactcgatg acaaggaata acgattggca atggattggt tcgatttgcc
    39721 tgcccaatcg ctcgcacagc tttcggacta tcaacagcaa cggctacttc tttatacgtt
    39781 cggatttctc cataaggaat ggtcgacaaa gctttccaga cattttgctg aaaaggcgta
    39841 cctctcttta tagataaagg aacatcaaat tcttttcttt ttcccgcaaa atattcttca
    39901 aactgttctt gtgtgcgcgt taaaattcgc tgattcgctg attcgctttc tgaaattggc
    39961 tgaaaagata cagcagtcaa tccggattca tctgcatcta accataaaaa gccgataggt
    40021 gtgtgtattt tcattttgtt tgctcctagt caatagactg gaacttcctc ctttttctct
    40081 atccaaaact acttgccaac taataaaaaa tgttcttaaa agtttgataa accgaggaaa
    40141 catggtaaac taaatacgtt agtttacaaa aagaatacct gttcttttac gtaaaatcaa
    40201 attttaaagg tgttatttaa ggtttattaa attttgaatg taaaccatat ataaacatac
    40261 gattgaaagg atagaaacga tgaaagtatc aattgttgtc ccatgtttta atgaagaaaa
    40321 aactgttcct cttttttttg atgaggttga aaaattcaaa ggtgcccatg agttcgaata
    40381 tttgtttatt gacgacggat caaaagacgg tacgttagca gcaatcaaga agctagcctc
    40441 agcttatcct tatgtccgct ttgtctcatt ttccagaaat tttgggaaag aagcggctct
    40501 gtacgctgga ttgcaagctt ctacaggaga tttagtgacc gtgatggatg ttgatctgca
    40561 agatccgccc gaactgcttc ctgagatgat caaccggatc actacttctg atatcgattg
    40621 tgtcggcacc cggcgttcga ctcgtgaagg cgagcctgcg atacgcagtt tctttgcgaa
    40681 aaaattctac cagttgatca ataaaatcag cgatacagaa atggtcgatg gcgctcgtga
    40741 tttccgcttg atgactcgtc aaatggtgga tgccgtccta gaattaacag aatacaaccg
    40801 attttcaaaa ggattattta gctgggtcgg atttaaaaca gaatatattt cttatgaaaa
    40861 ccgcgaacgt gtggctggcg aaacatcttg gtccttctgg aaattattca actactcgat
    40921 tgatggtatc gtgaacttct cggatgcacc gttaacgatt gcttcattta tcggcgcttt
    40981 gtcttgtgtc ggttctggta ttgcaatttt gttcattatt ttgcgggcat tgttgtttgg
    41041 tgatcctaca tcaggatggc cttccatggt ctcgatcttc ctgttcattg gtggaatcca
    41101 attgttatgt cttggaatca tcgggaaata tattgggaaa atcttcttag aaacgaaaca
    41161 gcgtcctgtt tatatcataa gagaaacaga aaaagtgaag ccaaatgaat tgaagcctca
    41221 ccaataatat acgtcaaatg aagtaaacat ttttttaaat gaaaaaggac tgtgacaaaa
    41281 gtcttgtcac agtccttttt tccgaataaa tggtgttcca gaagtagctt cttcggaaat
    41341 aagccgaaat ttcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga
    41401 agctgaccac ttctatcaca acctctttca tatcttatta cttcttcatt gctgctttta
    41461 gtgctgctgc taatgggctt tcttctggtt cttctgcttg tgcatatttt ttcagcaagc
    41521 gtcgttcttc atgtttggtc attttcttct tctgctcttt tttatcaagc aatttttctg
    41581 tcgttccatc gtatttacaa cggaaataag caccgttttt accttcgatg atttccattt
    41641 tccggtgaca ttgcgggcat cgatgattag agacttttgg atcttttctt ctgcggtagt
    41701 tgcaatctga attggtgcag acatagattt tgccgtcacg tgtgtttttc tcgcgcagat
    41761 tggccccaca ttctgggcat ttcttttggg taatcgagaa atcttgatat tcttgtttgc
    41821 ttgatttgat ctcattcacc aagcgttttg tttctttttc gattccattc aagaaggttc
    41881 ctgcttgttt ctttcctgca gcaatagctt caaggtcttt ttcccagctt tctgtcaaat
    41941 ctggtgttac taaagaggga tttaccaata ctaataattg cttaccttta ggagatactt
    42001 gcagtccttg aggggtacgt tccatcagtt cagatttgat tagtttctcg ataatctctg
    42061 cacgcgtagc tggggttccc agactgtatt tttccatttg accaagcaag cttgcttctg
    42121 acaatggttt aggcggtgca gatagttctt ttttgatggc gaaatcggga ttgattctca
    42181 ttcctttttc ccatttgact gtttccgtct cagtttcagt ttcgcctttc catcctgctt
    42241 ggacgacacg attttgccga aaaatgaatc gttctttacc aaattcaacc gtaacttttg
    42301 tttggctgac tttatggggt tgagcaaaca gtcctaaaaa acgagagaca atcatttgat
    42361 agatttttcg ctcttcatta cttaattttt cataacgtgc tctgttttca gttggcaaaa
    42421 gtgcatggtg atcggtcact ttactgtctt ggaatacttt tgtctgttgg acgatggctc
    42481 cgtttttcaa atactgcttg acctcaggag caaagtcgct gactgcttgc aatcgttctt
    42541 tcatcgtact tttcatgtca tttgtcagat atttgctgtc tgtacgcgga taagtaacga
    42601 tcttatgggt ctcatacaaa ctttgtacaa gacttaatgt tttctttgca gaatagccat
    42661 agcgttgatt ggcttctctt tggatctctg ttaaatcata aggcaatggc gcagattccg
    42721 tttttaattt ttcttggact tctgtgacag ttcctttgct tgtactgatc aattgaacaa
    42781 gtttttgtgc ttcttcttgt gtttttaaag caaattgatt tttttgaagc attttggctt
    42841 gttcttttcc agcttgcaac gtgatcgtga aatacgtttg tggacggaac tgctcgattt
    42901 tcttttcttg gtctctcact aatgctagtg tcggtgtctg cacacggcct gctgataaat
    42961 tatccgcgta cttcacagtt aaggctctgg tcacattcaa tcctaccagc cagtccgctt
    43021 ttgcccgggc aagagcagat tcatacaatg catcatattc tttcgcaggt cttagttgct
    43081 tgaatccagt tttgattgct ttatctgttt gagaagagat ccataaccgc ttcactggtt
    43141 tattgaaatg cacatactct aagatccagc gagcaacaag ttccccttct cttccggcat
    43201 ctgtcgcaat gactgcttct gagacatctt ttcgctgagc cagctgcttg attgctttta
    43261 attgatgtcc tgttttagga agtggtttga ttcctagttt tttagggatc attggcaacg
    43321 tgtccatttg ccagctctgc cattccttat tcagatcttc cggcattttc aatcctaata
    43381 aatgaccaag ggcccaagta acgatcacct tgggtccttc ataataattt ttgtgctttt
    43441 gattagcacc aagcaccttg cttaggtctt tcgcaacgct tggtttttcc gctagaatta
    43501 gttgtttcat aaaattcctt tctgctttct actgtgatgg gctgatgatt tttcaacaac
    43561 gcaagtccct tgtattgctg taaatcatcg tcacactctt cacaccagcg ctcatctccg
    43621 tcattccaaa aggcgctgag aaagttactt tttgcattga tatattcata attttgatgg
    43681 atttcgttcc attgtttttc gtacgcttgt cgtgcttctt cgaaagactc gaatgtttct
    43741 tcttcttcaa tatcttcttc ccagtcttca aaaaaccacc aaggctcatt gtccccgaac
    43801 atggtaatta cttgatacat actcgtgctc cttctttcag cttttctaga ctcatcttag
    43861 tcacttttca agaaagttgc aagaattttc tcttataaaa gggtcattta atgaacatct
    43921 taaaattgtt acaaatgaat ctgattgttt aaggcaaagt agcgataaag ttattgatca
    43981 tccaaaaata gctgtcttct ggatcgattg ttggttcttt tgtaaagtat cctaaaaaac
    44041 gtaaggaaag ataaccgtgg atcatactac gaagttccct gcttcgatga actttttctt
    44101 ctttcgttaa ttgaaaagcc tcaaatatct gcaaaatgat ctgattcgtt tcatggattc
    44161 catctaataa aacagcttca ttggtatgcg gtacactgat cagtaactca tacactgctt
    44221 gatttgaaaa agcaaaggat cgataagttt ctgcatagat ctgcagtgct tcctgaccac
    44281 tttttcccac aagagcccgt cgcagctggt cattaagttc ctgcagaagt ttcgctgtta
    44341 gcgctgtttt gacatcacga atatttttga aatggttata cagcgaggga gccttgatat
    44401 ccaagcgttt ggctaaatgc tgaaaggtta tttgctgaac atccatctct tctgctagtt
    44461 ctcgaaagca gtcgatgatt ttctcttgag acagatttct ttttttcatt tccatgtttc
    44521 ctctcataaa ataaatcctt cactctttca gtttaccacg aaaaagaaag agaaaaaaga
    44581 gactttcatt gataaactac atattgtagt ttataataaa gagctgtaga tgaaaatttt
    44641 cagaaagaag gagtctaatt ggctgcaaga ataaaggaaa aattatttag gaaaagagaa
    44701 acttgggaac aaaacttgtt tgtcttatgg tttggaacat ttatggctgg tatcggattt
    44761 agtttagtca tgccttttat gtcactttac atagatacgt taggtaatta tagtacgtca
    44821 caattgaact tttggagcgg attgactttt tcatctactt tcttggtgac tacgcttatc
    44881 tctccttggt ggggaaaatt agccgaccaa aaaggaagaa aattgatgct gttgcgtgct
    44941 tcattaggaa tggcaatcgt catttctttg atgggttgcg tgactagtgt gtatcaatta
    45001 gtcgcattgc gtctgcttca agggattttt tctggttata tcagtaatgc cactgccctt
    45061 gtcgcaactg ggacgccaaa agaaaaaagt ggtcaagttc ttggaacctt agcaactggt
    45121 tctgttacag gaacattact tggaccgctt cttggcggga tctctgcttc agcgtttggc
    45181 tatcgcccta cctttttcat tacagggatc atcttatttt tcgtcttctt gttgagtctg
    45241 tttttcgtcc atgaaaaatt tgtcccagtg aaaaaagaaa acatggtttc tgccaaacag
    45301 attttcaaag acttgaaata tccacacgtc gtcatcggta tgttcgtcac aacgatgatc
    45361 atccaagcat ccaacaattc cattagtcca atcatcagtc tgtacattcg ccaattgctg
    45421 catggacatg ggaatgttac gttgatcagc ggaatcattg cttcgatacc tgggattgcc
    45481 actttgatag ccgctccaag atttggtcgc ttaggtgaca agattgggag tgaaagaata
    45541 cttgcgattg gtttgggttt tgcgattctg gtttatatcc caatggcatt cgtcacgaat
    45601 gtttggcaat tagctgcctt acgttttttg attggtatct ctgatgcttg tctgctccct
    45661 gctgtccaag cattgatcac aaaatattcg cctcatcaag ctgctggacg gatcttcagt
    45721 tataaccaat ctttccaagc aactggtaac gtagtagggc ctatgatcgg ctccagtgtt
    45781 tccagtgcat ttggttatcg tggcgtcttt ctttcgactt ctcttcttgt tttggtcaac
    45841 ttcttgcttg tacgacacaa tacacaagag atccatcagc aagaaactgc agaagaaaaa
    45901 gcttcaggag tctccatagc ttcagggcac tctccttctc atagacaata agcgtaaatg
    45961 aaaaacactg cttgaacagt tgtcacagac tgtccttgca gtgtttttgt ttaggcctgt
    46021 tttttcaaga actcagataa gaggaataat cccttttcca aggtttcttt tttacaacag
    46081 taacctagtc gggcatgctt cggtaaatca aaggcacttc ctggtactaa tagtactcct
    46141 gtttcttcta ataaattgat acaaaatgtc tggtcatctt cttcgatggc taatttaatg
    46201 aaagaagtag agacgtaatt gggagcgaca agttcaactt tcggttcatt ggctacccat
    46261 tgttttaaaa tcacaaggtt ctctgtaatg atctttttgt ttcgcgccaa tattttttct
    46321 ttattcttca atgcatgtac agcaagatca tctgacagga ctccgccgca aatcatcgtg
    46381 tagtctcgat actttcgaaa aacttctgcc aactcaggtc cagtagctgt ccagccaata
    46441 cgaatacctg gaatggagta ggttttggat agggaattgg ttgctatacc tttttcatag
    46501 acatctacga tcgacaagaa ttcaccttta tccgttaaag gcgcatatac ttcatctatc
    46561 aatacatatg catctactgt acgagcaatt tctgcgattt tttctaaagt tttccgatct
    46621 agcaatgtgc ctgtaggatt gtttgcacta ttcaaacaga tgatttttgt ttcaggtttg
    46681 actaaggctt ctagctgttc taaatcaaac tgccaatctt cttcttcctt caaatgaaca
    46741 aagtcaacag cggcacctaa agatttgggg atgtcatata attgctgata agtaggaagc
    46801 atcgaaacaa catggtctcc tgcttccact aacgcgtata gagctaaaag attagcacca
    46861 gtcgctccat tcgtttgtaa gatgttttcc ggatcgacgg tttgataaag agaagcgact
    46921 tcttgtttga aaacatctga tccttcgatc cagccatagt ccaatggttc gtttttgcgt
    46981 ttttcgaaaa atttacttac gctagtccca tctaagccaa cgagttcgtc taatgtcaat
    47041 gcttcgatcg agctttgtgc aatatcatat accgctttct tttcccattt atttagccat
    47101 tcttctacac cgaaatttgc gattttcatg tttctttccc ccttatatct tgatccagcc
    47161 tacagcaagc aacccgattg aaagcagtgc ggccacacaa atcagcccaa tcattacttt
    47221 ttcttttccc gtaatttgat gtttggtttc acgacaagcg atccagtaga agcaaaaacc
    47281 aggaagatag gcaatactac ataaaaggac ataagaaaat cctgctaaaa tgatgcccat
    47341 caattcaaaa actacagcaa tcccaccgat cactacttgt tttttgattc cttcttttaa
    47401 agaaaactgg acttgataga tcccgacaag tagataacaa atcaaaattg ccgctgtaca
    47461 taaagaatag gcaaattgat aggcataatc agtaactaag aaagtcaata agaatagatt
    47521 agtcaagatt gcagtaacaa ctaacgaata agttggggca ttttttttat tcgtttttcc
    47581 ccaacctttc ggcaacagac cgtctcgagc catcaacatc gttgtttctg ctgggagcat
    47641 cgtccaagac aaccaagaac caatgatcga aataatcaag ccgatattga tcagcgctgc
    47701 tccccaattt cccacgattc cttttaaaat atttgccata gcaggttgtg aaatagttgc
    47761 taactcttct tgagataaga cgccgtatgg caagatcgaa gcaaggatat agataaacaa
    47821 taaacaaagc agaccaatga tcgatgcttg ctgggcttct gaacgtttct tcgcgcggtt
    47881 ggctaaaaca cttgcgcctt cgatccctac aaagacccac atcataacca tcaaacaacc
    47941 tttaatctgc aaccagatcg ttccttgctg ccctgttcca ttcactagat tatcagatac
    48001 gttaccccaa aaatcagcag tgaatatatt cactttaaat gcgataaata caaatattaa
    48061 aaataggaat aatggcacta atttacagat cgtcacgatt gtattgatga cgctcgctcc
    48121 ttcaactcca ttgtttacta ataaagtcag accccaaata aaaatacttg caaataaaat
    48181 agaaggaaca ttttgtccac ctttgaatat cgggaaaaat tctcccatgg cgctcatcag
    48241 catcgttgca aaagcaacat ttcccagcca cgcagacaac cagtaacccc aaccactgat
    48301 gaagccacct aatttaccaa atgaagcttc cgcgtaaccg aaaataccac catctagatc
    48361 tggacgcttt tctcctaaat gattcagcga taataccaaa gctaaaatac caatcccaac
    48421 gattatccat gaaaggatcg ctggccctgg agctgcagaa ctagccagat cacttgtgat
    48481 cccaaacaca ccgcttccga ttgacgaact gacaaccaga gcaaccagtc cccatttgct
    48541 aatacccgtc gatttctttt ccatttccat caccctcact tataaaattg aacaaatcac
    48601 ttgcaaaaga ttcccttcta ttttgcaact gctcttgctt aacaaaaagc aactttattc
    48661 atattattgg atattatgaa ctttttacac aagtatttca agtataaaat gaataaaaat
    48721 taattttatt gcaaaaaaac agctatgcta tccaaaagaa aaggtgtttc ttttgttagc
    48781 catagctgtt ttgattcatt taatatcgct taaacgtatt gtaaaacttt tgtttcttga
    48841 tatgcgtggt cgatcaaacc accacccaga cattccattc catcataaaa tacaactgct
    48901 tgtccaggag tgattgcacg tacaggttcg tcgaatacga cttctgcacg attatcatcc
    48961 aataagcgaa ctgtcacagg aacgtcttgc tgacgataac ggaatttcgc tgtacacttg
    49021 aattcttttg gcattggttc attcgtcgta aagtggattt cacttgcatc caagcttgtg
    49081 gcatacaatg caggatgatg gaacccttgc cccacatata atgtgtttgt cgctagatct
    49141 tttcctacaa caaaccaggg ttcttgcgaa tctcctccgc caccgatccc tagtccttga
    49201 cgttgaccaa tcgtataata catcaagcca gcatgttgtc ctttgacttc accatcaaga
    49261 gtgaccatat ttcctttttt tgctggcaga tagttgctca aaaattgttt gaagttcttt
    49321 tcaccaataa agcagattcc cgttgaatct tttttcttag cagtcgcaag gcctgcgcgt
    49381 tcagcaattg cccgtacttc tgatttttcc atgccgccta gagggaacat cgtttttgaa
    49441 agctgttctt gtgataattg gctaaggaag taagtttgat ctttattatt gtctactcca
    49501 cgaagcatgt ggaccacgcc gttttcatcg cgagtgactt gcgcataatg tccagtagct
    49561 acataatcgg cgccaagatc cattgcataa tccaagaatg ctttgaattt gatttctttg
    49621 ttgcacatca cgtctgggtt aggtgtacgt cctgcgcggt attcggctag gaaatattca
    49681 aatacacgat cccaatattc tttttcaaaa ttcacagagt aataaggaat cccgatttga
    49741 gcagctactt tcgctacatc cttataatct tccgtggccg tgcatacacc gttttcgtcc
    49801 gtgtcatccc aatttttcat gaatattccg atcacgtcgt atccttgttc ctttaacaaa
    49861 agtgctgtta cagatgagtc gacaccgccg ctcatcccta cgacaacgcg tgttttgctg
    49921 ttgtctgtca tgatatcacc atcatttcaa ttagattctg aaacatctac gagttgaagg
    49981 tcgcaacttt tcagtatttt acattatacg tcatatttct gaaaatttca tcccttaatt
    50041 atcaagtcgt aaaaatactg ttctactgga cctattcagt acacaaacat gatagtcaac
    50101 caaaaaatca ctcatctttt gagcattcga tataaaaaat tggcatactc ataaaaaggg
    50161 ccagcctttc ggcaataagg aataatttta taaattgtga aaaactattt ttcaaaattg
    50221 cttcttatcg cttaaagctg cacgtctcat ttacaacctt ttctttttag atgttatgag
    50281 ttgctcagat aagtgatttt gtttactcta agaaaaaagg gaggcaaaag atacatgaat
    50341 tactttatca gtgatatgca ctttttccat gaacgcttat taggaaaaaa cgactttgct
    50401 ccgcggctct ttgcaaatgt ggatgaaatg aaccaacagt tgatcactag ctggaatgca
    50461 gtaataaaag aaaccgacca tgtttatcat ctaggcgacc ttgctatgca tccgcaatat
    50521 gaaaaagcaa atgcagaaat tttggaactg gtcactcaac tgcaaggaac aatccacttg
    50581 atcaaaggga accatgacag tcgcgctttt ttccgttatt tagaaaaaaa tgatcctagg
    50641 cttactgacg gaagtcccaa attttatttt tatgatgtag gtgccatcgt taaattcgac
    50701 catcaacaat attacttgac tcattatccg atgttgctcg ggcctaacga cactatccgc
    50761 aatcttcatg gacatatcca tcattacagc gtacctatcg agaacgatgt caatgtaggc
    50821 gtagacgcac cggaaagaga actcttaaag gaaaaactac catttggcac tccgttatct
    50881 gagcaacaaa tcaaagaaat ctatgaagca aagaggaagt acgataggtc gtgaaaaaac
    50941 tgccctatat caagtattaa acaaataaag ataattactc atatttttca tttgagtagg
    51001 cttattgcca caggaacatt aggatcagaa atggagttgt aacaagactt ctgtcacaac
    51061 tccattccat actattcgtc tttttgctca tggtgttctt ttaccagttt tttctgtaat
    51121 tctttatcat aatttcgggc agctgacgtt aaaaataaaa cgattccgca taataaaatc
    51181 ccgattccgg aaaacaagaa cattttttct acccctaaac gatcagctaa tggtcctgca
    51241 aataataatc caattggacc tgtcaggttc atcaaagagt tgaagttccc taagacacga
    51301 cctaaaacat tcgattcata actctgctgg atcattgcca tcagcagtgt attgaaatat
    51361 ggcgttgcac aacctgctcc tgcattcaag ataagaaagt acaagaaccc ttgactatcg
    51421 tttggaacca atccgcttgc accgatcgtc aggcccataa tgatgtaggc tataataata
    51481 ggtttcattc ggtctttcca tgttcctata aaactgatca gtgcaccgcc taagagcatt
    51541 ccgactgcgt agactacttc cactgctccc gcctgtccaa cggaaccacc aaagtaatct
    51601 agcgtcatca atggatacag actgattgct ggcatgaaca acagcatcac aaatgcacca
    51661 ttgatcgtta tgtaccataa ccctttatta tccatcaatt gctgcagccc aaattttgca
    51721 tctttaaaaa tagtaagcgg aacttccaat atttcaggag aaacttttgg gatcttcaca
    51781 aaaatcaata agccgacacc aaaaactgcc cccaatacat ctaataaaat aaggtaattg
    51841 acaggaacga ctgaaaacaa agccgcacct agagctggcg caatgatgaa attcgctgat
    51901 tggaccattc ctaactgtcc attcgtcctt gttatgtgag aagatggaac gattgtcggc
    51961 ataatcgact ggatcgttgg catttggaat gtttgtgcga ccgaacgcat aaacaaagac
    52021 acaaaaacta gccaaatcgg gaaggattct gaaatcgttc cgactactgc taaaataagc
    52081 gcgaaaatcg ctacaataat atccgtgaca atcaaaagag cttttttatc ccagcgatcc
    52141 actagcggtc ctacaaatgg gcttagtaaa accatgggaa tcattcctag taaagtagca
    52201 aaactcaaaa ttgtcgccga accggtttct cttgtcaaat accagatgat cgcatattgg
    52261 acgaccatac tagtgattcc tgacaaaaac tgtcccgata aaaagaacaa ataatttctc
    52321 ttccaattcg ttaaaacgac cgactgttca ttttccataa acatccctct tttctttttc
    52381 attttgtata gcgtttgcac aacaatcatt atacgggatt cttttttcaa aaaatacctt
    52441 tttccctaaa taacaatcgt ttcaatttaa atttctttac ttagaaacaa taaatcagca
    52501 aaatattaaa aataacaaca tgtgttaaat ttaaaaataa aaaagacaac taatttgcgt
    52561 cgtccttaca aagttagtca tctaacaata ggccgtttca aaactagttg tctttttata
    52621 tccacagatc attcgacttt ttcgaataac ccacggctaa tcatcccatc catcagaaaa
    52681 taaaatttat cttggctcat tttctgtttt ggatctttga aaatattgac tgctttcaat
    52741 ccatttgctg tagtgatcga cattttcaat gtttgaatat cttttgatac agtgattttt
    52801 aatttgaatc cttctttgct ttgcggcgtg gcatctaatg gtcggatcaa ttgataattg
    52861 cctccgtttg tttctgtaaa tccattatcg cgcaatgtat atctttttgc atcaggatgc
    52921 aaacgatagc taattggtgc gcccagtacc gttgctgttg tttcaaatgc catgattttc
    52981 acctcgataa aattagtttc tctttattat aaaagtttat cctgattttt actaggtttt
    53041 tcagttaagt tccctaaaaa atgaaggcgt tctctcagac atctgttcat ataagaatga
    53101 cttcactagt tattttttga cagcacgaac taattgatca aggaaatact ggatgtcgtc
    53161 ttcggtattt cccaaaccaa agctgatacg gatggattct tttagtatcg gtgtatcttc
    53221 tccatacatt gcttccaata cgtgtgaagg ctcgatattt cctgctgtac aagctgaacc
    53281 agtagaaata gcgaccccat taagatcaag cttcatcagt agcaaatcat ttgcttcgcc
    53341 aattaagcga agattcaaca catgagggag tttattcgtt aaatcaccat ttacttgata
    53401 agggatttcg tttgcttcta atgtatcaag aatcaattgc tggaatcctt tatatttgtt
    53461 gttacgtgct tcacgttctt caggtgacag cagttcaact gcttttgcca taccccagat
    53521 ccctgctaaa ttttctgtac ctgcacgccg cttttcttcc tgctccccgc ctaaaagcaa
    53581 tgcagggata tttactccgt ctcgtttata taagaaaccg acgccttttg gcccattgat
    53641 tttatgagca gagatactta ataaatcgat tcctaattct tctggatgga tcgtttggtt
    53701 tccgtacgct tgtaccgcat ctgtatggaa ataagctgga tgatccgcta aaagttcacc
    53761 gatttctttg attggcatca aatttccgat ttcattgttc ccgtacatca cagagaccaa
    53821 gatcgtatct tctcgtaacg cttttttgac ctcttcgatc gacagattcc ctttttcatc
    53881 tacaggcaga tatgtaacgt caaacccctt tgtctctaaa tatttcatgg tattaagaac
    53941 agcaggatgt tcgatagctg tagtgattag atgtctgcct tctttttgac gcgagaacgc
    54001 tgtttctaag atagctgtat tatccccttc tgttccccca ctgttaaaga tgatctcgtg
    54061 aggttttgct tgcagactgt ctgcaatgat ttgtcttgct gtttccaatt tttcatgcgc
    54121 gtggcgacca aatccatgta tgcttgaagg atttccaaac gtgtcttgca tgattgccgt
    54181 catcgcttca attacttgtg gatgcatggg ggtagttgcc gcatgatcca gataaatagt
    54241 tttcacttca ttcatccctt ccaaattatt gctaggcaca ttctagcata aaatagcgaa
    54301 caaaccaaag aagagctcat ttttgtctag aggacgtttc atgggcatag aaaaaaggag
    54361 ctgtgacaaa agtcttgtcg cagctccttg aagctgctca cttctgtcac aacttctt
//
LOCUS       DAJSAW010000015        48709 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJSAW010000015 DAJSAW010000000
VERSION     DAJSAW010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.015"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            113..1183
                     /locus_tag="ONT64_000974"
     CDS             113..1183
                     /locus_tag="ONT64_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2302407.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            1198..1866
                     /locus_tag="ONT64_000975"
     CDS             1198..1866
                     /locus_tag="ONT64_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2302408.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            1886..2464
                     /locus_tag="ONT64_000976"
     CDS             1886..2464
                     /locus_tag="ONT64_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2302409.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            2867..3778
                     /locus_tag="ONT64_000977"
     CDS             2867..3778
                     /locus_tag="ONT64_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR2302410.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(4034..4951)
                     /locus_tag="ONT64_000978"
     CDS             complement(4034..4951)
                     /locus_tag="ONT64_000978"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCR2302411.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            5153..5590
                     /gene="zapA"
                     /locus_tag="ONT64_000979"
     CDS             5153..5590
                     /gene="zapA"
                     /locus_tag="ONT64_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR2302412.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            5648..6178
                     /locus_tag="ONT64_000980"
     CDS             5648..6178
                     /locus_tag="ONT64_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016630349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCR2302413.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELW"
     gene            6310..8670
                     /locus_tag="ONT64_000981"
     CDS             6310..8670
                     /locus_tag="ONT64_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCR2302414.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            8757..9071
                     /gene="trxA"
                     /locus_tag="ONT64_000982"
     CDS             8757..9071
                     /gene="trxA"
                     /locus_tag="ONT64_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCR2302415.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            9253..11031
                     /gene="uvrC"
                     /locus_tag="ONT64_000983"
     CDS             9253..11031
                     /gene="uvrC"
                     /locus_tag="ONT64_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR2302416.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            11176..12075
                     /locus_tag="ONT64_000984"
     CDS             11176..12075
                     /locus_tag="ONT64_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2302417.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            12068..13330
                     /locus_tag="ONT64_000985"
     CDS             12068..13330
                     /locus_tag="ONT64_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2302418.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(13424..14716)
                     /locus_tag="ONT64_000986"
     CDS             complement(13424..14716)
                     /locus_tag="ONT64_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR2302419.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSECRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            15000..16898
                     /locus_tag="ONT64_000987"
     CDS             15000..16898
                     /locus_tag="ONT64_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HCR2302420.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(17197..17916)
                     /locus_tag="ONT64_000988"
     CDS             complement(17197..17916)
                     /locus_tag="ONT64_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HCR2302421.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            18253..18585
                     /locus_tag="ONT64_000989"
     CDS             18253..18585
                     /locus_tag="ONT64_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302422.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            18572..20575
                     /locus_tag="ONT64_000990"
     CDS             18572..20575
                     /locus_tag="ONT64_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HCR2302423.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            20594..21064
                     /locus_tag="ONT64_000991"
     CDS             20594..21064
                     /locus_tag="ONT64_000991"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HCR2302424.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            21085..21669
                     /locus_tag="ONT64_000992"
     CDS             21085..21669
                     /locus_tag="ONT64_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HCR2302425.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            21684..22670
                     /locus_tag="ONT64_000993"
     CDS             21684..22670
                     /locus_tag="ONT64_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HCR2302426.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            22660..22971
                     /locus_tag="ONT64_000994"
     CDS             22660..22971
                     /locus_tag="ONT64_000994"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HCR2302427.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            22993..24774
                     /locus_tag="ONT64_000995"
     CDS             22993..24774
                     /locus_tag="ONT64_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HCR2302428.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            24767..26143
                     /locus_tag="ONT64_000996"
     CDS             24767..26143
                     /locus_tag="ONT64_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HCR2302429.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            26150..26785
                     /locus_tag="ONT64_000997"
     CDS             26150..26785
                     /locus_tag="ONT64_000997"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HCR2302430.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            26815..28170
                     /locus_tag="ONT64_000998"
     CDS             26815..28170
                     /locus_tag="ONT64_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HCR2302431.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(28362..29756)
                     /locus_tag="ONT64_000999"
     CDS             complement(28362..29756)
                     /locus_tag="ONT64_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR2302432.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            29848..30819
                     /locus_tag="ONT64_001000"
     CDS             29848..30819
                     /locus_tag="ONT64_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HCR2302433.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            30816..31598
                     /locus_tag="ONT64_001001"
     CDS             30816..31598
                     /locus_tag="ONT64_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HCR2302434.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(31767..32138)
                     /locus_tag="ONT64_001002"
     CDS             complement(31767..32138)
                     /locus_tag="ONT64_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2302435.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            32272..32715
                     /locus_tag="ONT64_001003"
     CDS             32272..32715
                     /locus_tag="ONT64_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCR2302436.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(32758..33567)
                     /gene="yidA"
                     /locus_tag="ONT64_001004"
     CDS             complement(32758..33567)
                     /gene="yidA"
                     /locus_tag="ONT64_001004"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR2302437.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(33586..34953)
                     /locus_tag="ONT64_001005"
     CDS             complement(33586..34953)
                     /locus_tag="ONT64_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR2302438.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(34937..35767)
                     /locus_tag="ONT64_001006"
     CDS             complement(34937..35767)
                     /locus_tag="ONT64_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCR2302439.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            35930..36367
                     /locus_tag="ONT64_001007"
     CDS             35930..36367
                     /locus_tag="ONT64_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCR2302440.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            36443..37063
                     /locus_tag="ONT64_001008"
     CDS             36443..37063
                     /locus_tag="ONT64_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCR2302441.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            37252..37758
                     /locus_tag="ONT64_001009"
     CDS             37252..37758
                     /locus_tag="ONT64_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCR2302442.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            37775..38674
                     /locus_tag="ONT64_001010"
     CDS             37775..38674
                     /locus_tag="ONT64_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HCR2302443.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            38761..39645
                     /locus_tag="ONT64_001011"
     CDS             38761..39645
                     /locus_tag="ONT64_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR2302444.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(39837..39908)
                     /locus_tag="ONT64_001012"
     tRNA            complement(39837..39908)
                     /locus_tag="ONT64_001012"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39873..39875),aa:Arg,seq:ccg)
     gene            39968..41302
                     /gene="rpoN"
                     /locus_tag="ONT64_001013"
     CDS             39968..41302
                     /gene="rpoN"
                     /locus_tag="ONT64_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR2302445.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            41451..42542
                     /locus_tag="ONT64_001014"
     CDS             41451..42542
                     /locus_tag="ONT64_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HCR2302446.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            42583..43584
                     /gene="gap"
                     /locus_tag="ONT64_001015"
     CDS             42583..43584
                     /gene="gap"
                     /locus_tag="ONT64_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR2302447.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            43694..44884
                     /locus_tag="ONT64_001016"
     CDS             43694..44884
                     /locus_tag="ONT64_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR2302448.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            44988..45743
                     /locus_tag="ONT64_001017"
     CDS             44988..45743
                     /locus_tag="ONT64_001017"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR2302449.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            45859..47157
                     /gene="eno"
                     /locus_tag="ONT64_001018"
     CDS             45859..47157
                     /gene="eno"
                     /locus_tag="ONT64_001018"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR2302450.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            47556..48695
                     /locus_tag="ONT64_001019"
     CDS             47556..48695
                     /locus_tag="ONT64_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCR2302451.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        16124 a         8462 c        10381 g        13742 t
ORIGIN      
        1 aaaaattcat ataatgaaat agcggaagaa ttttttgagc ataatattcg ccaaaaattt
       61 atcaagtgat aaaattgttg tataaaatga ttatttcagg aggaggagtt aaatgttttt
      121 agcattaaat gaaattatgc attcaaagtt acgttatgcg ttagttgcag gggtgatgtt
      181 tttgatcgcc tacctggtat tttttttgac tgggttagct tatggcttag ctcaagataa
      241 tcgaacagca gtcgacaagt gggaggcaga ttcgattgtg ctttcgaaag atgcaaattc
      301 aaatctaggg atgtccatga ttactaagaa gatagcagaa gaagtcgaag gtgggaaagt
      361 ggcgtatctt gcccagacac ctggagtcgt gactagtaaa gacagtacag aagaaggaaa
      421 aatcaatgtt agcttcttcg gtatcgataa aaatcaattc atcatgccca atcttgttga
      481 aggaaaagct tttgataacg atgatgaggc agtaggcgat attagtttga aagaagaata
      541 tggtctagcc gtaggagata cagttaagtt atctggtagt gacaagacgt ttaaactaac
      601 tggctttacg gatcatgcca aattcaatgt ctcaccagtt ctctatacaa ccatcaatgc
      661 ttaccaagag atccgcttcg aaaaagaaga tacaagtgaa aatgcacgga ttaacgcaat
      721 agttgtcaga ggtaaaatca gtaatttacc tgaagattta gaacagatca aaatttctaa
      781 attcatcaat gaattacctg gatataatgc gcaagtattg acttttggat ttatgatcgg
      841 atttttgatc gttattgcgg ctattgtgat cgggattttc atttatgtac tgacgatgca
      901 aaagattaat atctttggtg taatgaaagc acaagggatc acaggaggat ttattgcgcg
      961 ttctgtagtg gcacaaacat tcattttatc atttgttggt attcttcttg gtctattagg
     1021 aacagtggga acatcccttg ttctacctga tgcggttcca ttccaaagca attggctgtt
     1081 ttttggcgtc attagcttgt tgatgctggt agtagctgtg ttaggcgcac tattttctgt
     1141 tcggacaatc gtaaaaatcg atccgttgaa ggcaattgga taagaaggag gaagagcatg
     1201 aaagcaatag aatttaaaga agtagaaaaa agtttccaag atggcgacca agtagttaaa
     1261 gcattgaaag aaacaaccat gagtatcgat aaaggggaat ttgttgcaat catcggtcct
     1321 tctggttcag gtaaaagtac ttttttgact ttggcaggcg gattgcagac gcctagcagt
     1381 ggctctgtat ttatcaatga tcaagaattc agtaatcaaa aagaaagtga acgcgttaaa
     1441 ctacgtttta aagaaattgg ctttattttg caagcttcca atctggttcc ttttctgaca
     1501 gtaaaaaagc agttattatt agtagataag atcaaacatg aacatcgtca aaaagaagca
     1561 aaagaattat ttgagcagct tggcgtggat aagttgatca acaagtatcc agaagaacta
     1621 tctggtggag aacggcaaag ggtcgcaatc gctcgtgcat tgtacaatga tccgtccatt
     1681 attctagcag atgaaccaac tgctagtttg gattcagaaa aggcacgaga agtagtcgat
     1741 atcttggcaa aagaatcaaa agacaaaaat aaagcaacga tcatggttac tcacgataca
     1801 cggctgattg acaaatgcga tagaatattt atgatggaag atggtgtatt gaaagaaaaa
     1861 gaataaccaa aaagcaggtg gaaaaatggt ccatcgatac aacaaagata cacagcaaaa
     1921 aattttgaaa gaagcgactg acttgtttat gtccaaggga tatctcggca cttcaacaag
     1981 agagatcgca caaaaagttg ggatcaccca acccaatctc tatcattatt tcggtgataa
     2041 agaaaaacta tatacggcag ttttagaaaa ccacttaaaa gatgttggaa aagctttgag
     2101 agaaatcgtt caaacaagcg aaacgggatt tcaagtaacc ttgacaaaaa tggcgcagtt
     2161 tttgattgag acacatctgg tcgatttgtt tctgatgctt cacgatcttg aaagcaatct
     2221 ttcaaaggaa acaagagatc atcttttctt tctatggaag aaaaattatc gtgaaccttt
     2281 tgaagaaatc ttttcagcaa accaatctgt gctaagaaat gggatttcaa aagaaatcgc
     2341 agcaaggcat tttttcttag tgttagcacc ttacatcact cggtcgaaaa gacctgtgga
     2401 agaaccgcta aatgtggcgc aattgatcga tttatatttg aatggtgtat taggagaagc
     2461 gtaaagctgt tatataggga gaaaaatagt taggtgaaga agcaagatat gacgaagatc
     2521 gttatatctt gtttttttta tataggtagt ttttcataaa tacaaatttg ttattctctc
     2581 atgattatca gggtatattt ttatttatta tgtcggtgtt ccaggaagtg ttttaattta
     2641 ataacgaaga aattaattgt ttgtagaatg aaggaaacta tttattgggt agatgaatca
     2701 ccacgaatag tcaaaaggag atttcaggat atgccaaaaa tgaaaaaaat cattttagca
     2761 gtaatgttat tttctacggt tatatctttt acagcccctt ttttgcgtac ttaagctgat
     2821 tttaagtttt gacccttagt ctggatctac gaggtgagag tcaagaatga aaaaaataat
     2881 tggagctttt gtatttgcaa tcattatcat tattatctgt tttcaaatag atgtttttgg
     2941 tcaaaaaaag atagttggac aaacggtcag cagtacgaac agtacggtta caaagacaaa
     3001 ggaatcattt gatgatacag aacaagagac attaatcaaa atcggaaaaa atgaccgtac
     3061 actatataca gatgactcgc taactacccc acttgcaact ataaacggcg gagagctggc
     3121 ggagtttctt tctgaaacaa aaaatgctta tcagatcaag acaaatgatg gctatactgg
     3181 atatttagcc ctcgaagatg gaaccaaagt cacaaagaat atacaaacaa aaccggaaga
     3241 gctatctgat gcagtgattg tattagatcc tgggcatggc ggaaatgata ctggggcatt
     3301 aagcaatgat gaacaaacag aagaaaaaga tataacatta agtacagcca taaaagtaaa
     3361 aaaggcgcta gaggatgctg gagcaacggt ttatttgacc catactacag atgaattagt
     3421 acagcttgga gatatatgcg actacagtga agaaaaaaag gcagatgtct ttatcagttt
     3481 acacgcagac tctacggaat atgcgaatga agcaactgga atcacgacat attattacta
     3541 tggacaagag gaaacattag cacagacaat tgctgactcc ttcactgatc tgcctctgga
     3601 ttcaagagga atctccactg ggaactatca agttcttcga gaaaatcttc aaccttctat
     3661 tttaatcgag atgggttata tgaataatga tagtgattta aaggaattag taacagacag
     3721 ttatcagcag cagattgctg caagtttgac acaagcattg accacttatt ttcagtaata
     3781 aataagccca gccaaacaaa aatagctcct catatttttc gtttggccgg gcttattatc
     3841 gcagatgcaa gcttttgaac accgtttaag gaagaggttg tgagcctgcc gtttaactcc
     3901 gcgtcacaag gagcaatttc aaaaaacagc ttctcatgtt tgatgactat tgtgacttgt
     3961 gaccgaggag ttggcatgag aacaccgttt attcggaaca agggactgtg acatgacttt
     4021 tgtcacagtt ccattatttt tttagcagct tcaaagcttt ttctgtattg gcaaaatgaa
     4081 gtttggcata tttttcaagc ataggcatgc cgcctttctc caatagtttt gccgctactt
     4141 ggtcagattt cgttccagct cctgatggaa gggtgattcc agcttcagct gatgctttgt
     4201 gtaactcttc caaaaagctg gcacgactga tgatagaagc agcagcaaca gctaaatgat
     4261 attgttctcc tttagtaacg aagtacagtt tttcttgtaa ttgatttttt tctaaacgcg
     4321 catatttttt aaaatttgct tccggcgtaa actgatcgat caaaatcgct tctggttttt
     4381 ctggctcgat tttttttagc agcaaatgga tcgcttgatt gtgcaaagcg actttcatcc
     4441 gaacggcatt gtattttggt tggatctcat tgtatttttc tggagataag acaagcagtt
     4501 tataggggat ggtttttttt aagatgtcag caagctggat gatttggcta tcttttaact
     4561 cttttgaatc tctaacacca agtttacgta attctgcaag ctgatcacgt ttcacataag
     4621 cagcacatac tgttagcgga ccaaaatagc tgccatttcc gacctcatca ctacctaaaa
     4681 cggataattg actgatattt tttggcaaag aagaggctgt ttttgctggc ttggcttgtt
     4741 tatcctgtgc ctccccccat ttatcagctt ctattgctgc attcgtacct tgaaacatca
     4801 cttttccgga tgtataagcg gtaatggtcg tcccgtcttt ttttgcagca aagctagtat
     4861 aaggaatctg tttatttaac aaataagagc gatagtatgc tttcatttga ctaagttggt
     4921 cgtttgttgc tttgataatg acgttgctca taaaattctc ctcgttttta tacataaaac
     4981 tataccgcaa ctcacgactt ttcgttttct aagagattgc gagacatagc atttttttaa
     5041 agtttatcat ataatagtta agtatgtgaa aaatatttgt ggagaacggt gaaagaacca
     5101 acttcatggt acactaaata aaactttcat caaataggag gaaatgattc gtatggcgca
     5161 cgaaaaaaca cgctacaaag cagtgattgc caaccaaaca tatactatta tcggacggga
     5221 aaccaagcat catatggata tcgtaacgaa gctgatcaat gaacagttag cagaattgaa
     5281 acaattatca ccacaaatgg ataatgaaca agcagcaatc ttgatggctg tcaatgcgct
     5341 ttcggatcaa ttgaagaaac aagaaagaat cttggagctg gaagaagaaa cagcagaatt
     5401 aaagaaaaaa atgatcaaat ttacagagct tgaaaatcgt gtgaaacgca ttgaagcaat
     5461 cgagaacgaa gctcgggaag tattgaaaga aaatggtcaa gcagatcata ccattaaaaa
     5521 tcatgtggaa gcacaacaga ttttaaacga gaaacgaaaa ggacaaatta aacaaaaatc
     5581 ttctaattaa gatcctttta tcaaaataag aaaaatttaa aaaagttaga agagaaagga
     5641 acgtagcatg ctgagtttgc tgattttatt tttattgcta attgcgtttt tttctggagc
     5701 acgtcgcggg tttgctttgc aaatcatatt cgcgattgga tatgtattgt catttattgc
     5761 tgcccagcat ttttacaaac cattagcaag tcatttggaa ttatacattc catacccagc
     5821 tgtcacccca acatcaaagt tagcattttt tgatcaggta tttgcttttc acctagatga
     5881 agctttttat gcaggaactg cttttctgat tattttgcta attggctggc tgcttacccg
     5941 atttgtgggg gtttttgtcc atggattgac gtatgtgccg atcttgagac aggtagactg
     6001 gattgcagga ggcatcctgt cactaatcat ggcttacgtt actatatttt tgattttgca
     6061 gttacttgcc tttgttccat tagatgtggt ccaaaatcaa ttcggcgagc atagtttcgc
     6121 tcgttttatc gttgagagaa cgccatttct taccaacaag atgtatgagc tttggtaacg
     6181 aatattttga attaaggaaa atagtgctgg aaaatatttc gttttttgta cagagtagtt
     6241 tatttaagaa ggctgcgtag cgtatgtccg gcacagctct tttttcatag tgaaagataa
     6301 ggtgataaaa tgaataaccg aatccttgaa acattggaat ttgaaaaagt caaacagatg
     6361 gtcagacaat ttgtagtgac tgcacaagga aaagaagaac tggcagaatt ggtaccagtg
     6421 agtgagaaac aaacgatcac aaactggctt caagaaactg aggatggatt gaaagtgcaa
     6481 cgtctgcgag gcgggatccc tatacctaaa cttgaaaata tccgtcccca tatgaaacgg
     6541 atcgagatcg gagcagactt gaatggagtg gagcttgcac aagtttccag agtattgtct
     6601 acgacaagcg aattgaatcg tttcattgat gatctttctg acagtgaaat cgaatttgca
     6661 cgtttgtata tgtgggcaga tcagttagtc acgatccctg ttttgagccg ccgtttaaag
     6721 gaagcaatcg atgaagacgg acgcgtgact gatgacgctt ctcctgagtt aaagagcata
     6781 agacagaaca ttcggcgaag tgaacaagct gttcgtgaac agttagatgg gatcgtccgt
     6841 gggaaaaatg caaagtattt aagtgatgcg atcatcacca tgcgaaatga ccgctacgtt
     6901 atcccagtca aacaagaata ccgcggtgta tttggcggtg tagtccacga tcaaagtgct
     6961 tcaggacaga ctttatttat tgaaccaaaa caagtggtgg atttgaataa tcgtttgcgt
     7021 caataccaaa tcgctgaacg aaatgagatt caacgtattt taagtgaact ttctgctgaa
     7081 cttgttcccc atcgtcaaga aatcatccac aatgcttatg tgattggtaa aatggatctg
     7141 atgaatgcaa aagcacgatt tggtaaagaa gtaaaagcca tcgtgccggg aatcagtgaa
     7201 gacaatcatg tcgtcttgaa acaagcaaga catccactga tcgatcagga aaaagtagtt
     7261 tctaatgaca tcacaatcgg aaaagactat caagccatcg tcattacagg acctaacaca
     7321 ggtgggaaaa cgatcacgtt gaagacttta ggtctgttac aactgatggg gcaagcagga
     7381 ttaccgattc ctgcaggtga agaaagtcaa atcggtattt ttgaggaagt atttgcggat
     7441 ataggagacg aacagtcgat tgagcaaagt ctatcgacct tttcttctca tatgacgaac
     7501 actgtagata ttttatcaaa agtcaatgaa aaaagtctgg tattatttga cgagctcggt
     7561 gcaggaacag atccgcaaga aggtgctgct ctagcaatcg caatcttaga tgatcttggt
     7621 aaaaagtctg cttatgtgat ggcaacaacg cattatcctg aattgaaagt ttatggatac
     7681 aatcgggcaa atacgattaa tgccagcatg gagttcgatg tggatacact aagtccaacg
     7741 tatcgtttgc tgattggcgt tcctggacga agcaatgcgt ttgaaatctc tagccgatta
     7801 ggtcttgata cagaagttat tgatgaagcg aagcagttga tgaatgatga gagccaagat
     7861 ttaaacgaaa tgatcacaga tttagagaat cgccggaaaa tggcagagac cgaatatctg
     7921 gaaatgcgtc attttgtttc tgaggcacaa gaacttcacg atgatttgaa agaagcttac
     7981 agctatttct ttgaagaacg tgaaaaagaa atggaaaaag caaagaaaaa agcaaacgaa
     8041 gtcgtgtcag aggcggaaga aaaagcagaa aaaatcattg ctgatattcg taaaatgcaa
     8101 cagcagattg ggcaaggaaa tgtgaaagag catcagttga tcgatgcaaa gacccaatta
     8161 gcaaatctac atcaagaaga aactttgaag aaaaataaag tattgaagaa ggcgaaagaa
     8221 caaaaaacgt tgaaaccggg agacgaagtg ttggttacga cttacggcca aagagggacg
     8281 cttttacgta aaaacgggaa tcagtggcaa gtagaaatcg gtatattaaa aatgaatgtg
     8341 tccgaagacg agctgacgcc tgttgctcct caaaaagagc cgacacaacg agttatccat
     8401 gctgtacgat ctgaatctag tagtcacgtg ccgaatcaat tggaccttcg aggaaaacga
     8461 tacgaagaag cgctaagtga ggtagatcaa tatttggatt ctgccattct agctggttat
     8521 cctcaagtga ctattgtcca cggaaaagga acaggcgcac ttcgcaaggg aattactgat
     8581 tatcttaaaa accatcggag cgtcaaaagc tttgagtttg caccggcaaa ccaaggcgga
     8641 aatggtgcga caattgttaa atttaagtaa agcttactaa aggtaagcag atggttcgct
     8701 atccaagtat agaaatgcta tactaatcat agattttaaa aatggaggtc tttgatatgt
     8761 cacaagtaat tacagataag acatttaatg aagaaacaga taaaggactt gtattgattg
     8821 atttttgggc aacttggtgt ggtccatgtc gtatgcaagc accgattttg gatcagttgg
     8881 aacaagaata tgatgaagaa gaattcagaa ttgctaaaat ggatgtggat gaaaatccag
     8941 aaactcctca acaattcggt atcatgagca ttccaacatt aatgttgaaa aaagatggtc
     9001 aagtagtaga aaaagctgtt ggtgttcatt caaaagaaca actacgtcaa atgatcgatc
     9061 aatatttata aaaacttaca gcaaaaataa gctgagggtg ggactgaatc cagttccgtc
     9121 ctcggttttt ttgcggttga gttaaggtta ttcaaattaa tattttttga gtataattac
     9181 gttgtcaggc tggattgttt ttttgacaga catgctataa taactttaat tgaatatgaa
     9241 ggggagtacc agatgaacga acgaatcaaa aacaaattag ctttacttcc tgatcagccc
     9301 ggctgttact tgatgaaaga taaaaatgga acgatcattt atgtcggcaa agccaaaata
     9361 ttaaaaaatc gtgtccgctc atattttact ggcagccata atacgaaaac ggaacggctt
     9421 gttagtgaaa tcgaagactt tgaatacatc gttacagaat cgaatatcga agcattgctc
     9481 ttagagatca atctgataaa gaaaaatgat cctaaataca acattatgtt aaaggatgat
     9541 aaaacctatc ctttccttaa gataacgaat gaaaaatatc cccgtttggt gattactcgt
     9601 aaagtactaa aagacaaagc actgtatttt ggaccatatc ccgatgtcgg tgcggcgaat
     9661 gagacaaaga agatcctcga tcgattattt cctctgagaa aatgcaaacc tagtcagacg
     9721 aaggagcctt gtctttacta tcatttggga caatgtctgt gtccttatta tttcgacgta
     9781 gatccgaaag tctatacgaa aatcgttgat gaagtgaaaa agtttttaaa cggaggacac
     9841 gaaaaaatcg agtcagagat ccatcagaaa atggaaaaag ctgctgaaaa catggagttt
     9901 gaaaaagctg cagaataccg agaccagatc cgtgccattg agacaatcat gactcgccaa
     9961 aaaatgacaa atactgattt actggatcga gatgtatttg gttatgcgat tgacaaagga
    10021 tggatgtgtg tccaagtgtt tttcgtaaga caaggaaagc taattgaacg agatgtttca
    10081 atgtttcctt tctataacga agcagaagaa gatttcttga cttacctcgg tcagttttat
    10141 caggaaaatg agcatttcat tcctaaagaa gtcttgattc ctgatacgat cgacaagccc
    10201 agtgtagaag ctttgttagc tacgaaagtg ctgcaaccaa aacgaggaga aaagaaaaaa
    10261 ttagttgaat tggcgaataa gaatgcctca gttgctttga atgaacggtt tgatctgatt
    10321 gccagaaaac aggaacggac gatcggagca gttgaacgtt taggaaacgc gatgaatatc
    10381 ccgaccccaa tacgtatcga gtcttttgat aactctaata tcatggggac gaatcctgtg
    10441 gctgcgatgg tcgtattcat tgatggaaaa ccggcaagaa aagaatatcg gaaatataaa
    10501 ataaaaacag tcaacggacc ggatgattat gcatcaatga gagaagtcat ctatcgcaga
    10561 tattcacgag tgattcgcga agggttgcct ttacctgatt tgatcttgat cgatggagga
    10621 aaaggacaag tagatgtggc aaaagatgtc ttagaaaatc aattaggaat cgatattcct
    10681 attgctggca tggcaaaaaa cgataaacat aaaacaaatg agttgctatt cggtatagat
    10741 ttggctgtag taccgcttga gcgaaattca ccagagtttt tcttactcca gcgaatccaa
    10801 gatgaagtcc atcgattcgc aatcactttc catcggcagc tacgcagtaa aaacagtttt
    10861 gcttcccgat tagatgaaat tgaagggtta ggaccaaaac gaaaaaaaat gctgcttaaa
    10921 gagttcaaat cgttgaaaaa tatcacagca gccacgacag aagaattaca agaaatcgga
    10981 ctgccgaaaa atgtcgcaca aaacgtattt gataaattac atcaggaatg aggaaagggc
    11041 actttacaaa gaaagtaaag tgtcctatca ttttatgtac aacaaaaaga gcgtttacgt
    11101 tttgttggta aataataaat cgcttacgtc tggtgtctaa ggatatatgt gatttcttga
    11161 agaaggagtg aagaaatgtt agaagtcaag gatcttgtta aaacttttgg gaactacaca
    11221 gctgtcgatc atgtctcttt ccaaatcccc gatggaaaaa tcatggggct aattggccaa
    11281 aacggggctg ggaagacaac gacttttcgt ttgatcttgg attttttgac acaagaccaa
    11341 ggagaagtgt tatggaatgg ccatcatctt ggggagaaag attatgatgt catcggttat
    11401 cttccggagg aacggggact ttatccgaaa gtaacgattc aagaccaact gctttatttt
    11461 gcagaactta gagggaaatc aagaaaagag attgaaccaa aaatcgattt ttggatggaa
    11521 aaatttcagg taaaagggaa aaagacggat aaagtaaaat ctttgtctaa aggaaatcag
    11581 caaaaagtcc agctgatcgc cacgttaatc catgaaccta aattgatcat tttggatgag
    11641 ccattcagcg ggttagatcc ggtaaatgct gaattgctaa aagatggaat attggaattg
    11701 aaaaaacatg gttcatgtgt cattttttct agccacaata tggataatgt agaaaaaatc
    11761 tgcgatcatt tgatcatgtt gcgaaacgga aaaatggttt tgaatggaaa agtacatgag
    11821 attcgtgaat catttggccg tacgaagtta ttcttggaat ctggtttaag ccaacaggaa
    11881 gtagaagaaa tcgaaggcgt gaaaaaagtg atccttcatg aagacaatac gttagagatt
    11941 accttagaag atcctgaagc tgggaaagaa atctttactc gtgcgactca gttcggctac
    12001 attcctatgt ttaaccagca accgccaact ttggaagaga ttttcaaatt gaaagcaggt
    12061 gaaatcgatg cgtaagtttt ggattattac caaagatgtc tatttaaaaa atgttaagtc
    12121 gatttctttc ttgatcatga tattagtccc ttttatcatg ctgggtatca tttatgtagc
    12181 aggaaacttt gctcaacaaa atagtgatgt caataaaatc ggcgtattgg ctgataatga
    12241 gcaaatcagt cagcaaatga gccaagtcaa aacagatgat tttgagttca aagcagttga
    12301 ttctgaagaa aaagcacaaa aagagctttc ggaagaaaaa atcgatgcgt atttagtagt
    12361 ctcaactgag aatgataaga taaatgggat gctttatagc gaaaattcat taggacaaag
    12421 tacacaacta ctgatacaac aacaactgac tggcttgcaa tcgatgcttc gagcaagtca
    12481 attaggcatt tcaccagaag aagtagctag cttgagccaa caagcaagtt tgactcgaca
    12541 aaaagtcaat tttgattcaa aaggaaaaat gacagttgga gaagataact cgacagtcca
    12601 atatgtcgta agttatgtag caacgattgt cttgttcatt attattttga cgtatgcgca
    12661 gatcattgca caagagatcg cttctgaaaa aggcacgcgg atcatggaag tgatcctgtc
    12721 cagtacgact gcacaaaagc atttttacgg aaaactaaca ggtgttttac ttgttgcatt
    12781 aacgcaaatg gcattatacg gtattatttt tggtatagga tacagtcaat tcaaagatat
    12841 ggaaatggtc aaaaatatat tagacggtat ctcattggat aacttgattg gaccattctt
    12901 gtggtattct cttctattca tgatctttgg cattttgatt ttttctgttt tagcagcttt
    12961 atgtggctct ttggtcaaca aagcagaaga tacagcaaaa gcaattttgc cagtgactta
    13021 tctatctcta ggcggatata tgcttggatt gatactagga gcttcagatc caaacaatat
    13081 cgttattcgg atcacttcgt atattccatt cttatcttca tatattatgc cggttcgctt
    13141 agcaaatgag acagtcggta tgggtggagc attgatttcc ttgttggtac ttgtagccgt
    13201 aacattctta ttgatgatct tgtctgcaaa catgtacaaa tcgaatgtac tcgtgtatag
    13261 cgaaggcgga ttatggagtt cattgaaaca atcgatttca attatgagaa atgaacgaaa
    13321 aaaagcataa aatatagaaa aatggctctt tgtcaaatga tgttggtaaa gagaaatctt
    13381 agaataattg gagatcaagt ggatcagttc ctcttgatct tttttagttg aaaatcaatg
    13441 cgttgccatg acgaatcatg atgcgcaatt tgaaattata aaagctacga aagccatatg
    13501 catttcgttt taacactttg atatggttgt tcagacattc aagtgggcca tttgaataac
    13561 tttgttgtaa agcatttttg atttgtggca gaaatttctg gaacgttctc aaggtcgtct
    13621 gatagccttc gggaagcatg atattattgg taagtagttc ttcaaacgat acgtagtctc
    13681 gttcctttac agcgtaaaga aagtcttgat ataactgata gcctacacgc aaagcagggc
    13741 tccctttcag taagcgatcc acaatatcgg tctctgttaa atgagctttg aagccaggtc
    13801 gccaaaggcg tcgagtataa tcaagcttgc gttcatcttt ttgaagaagt ttccagtatc
    13861 tttttaattg cttacctaat tgatagtgtt tttgttcttt gctttttaat agttggttcg
    13921 tttctttgat tcgatgattg cgaaaggtac gaccaatatg ttggacgatg tgaaaccggt
    13981 caatgatcag ctgtgctttt ggaaacagct ttttaacgag cgatacataa ggagcgtaca
    14041 tatcaatcac gatccattcg accgcttcac gaatttctcg tgaaaaatga ctaaaataac
    14101 gttttaaaaa aggcagttgt cggttctcga caatgtccaa caagcgctgt gtttgtccat
    14161 ccatcatgat gaaactcata gcacctgaaa caccccgaac tgatttgaat tcatcaaaac
    14221 aaagtacctt aggtaacggt gtccgaaaag gattcaacgg acgataaaat cgcttcataa
    14281 tacggtaaac agaagagacc gatagatttt tctgagcggc gatctctgtc attgaggtat
    14341 tttttttcag acgagtcacg acagaccagc ggactttctc actgatgaaa cagtgttttt
    14401 tcgcaacaga agtatctgcg acaaaggtcc gctgacaagc tttgcatttg aagcgttgct
    14461 ttttcaattc gaggtaggta cggcattcag aaacatcgtt cattaacaac cggacggttg
    14521 ttgtacccca acgcacaata tctgaagcaa tctggtgttt acagtggatg cattcttggg
    14581 ctgtataaat caagtttcca cgataaatat gacagatttg ccctttgtat ttttcttttg
    14641 tgagacaatt ttcgttaaaa gtaactgata agtctagaat atctaatgtt tctttgataa
    14701 ggtgagtgta agacatcttg attcctcctt ttgggttggt tcgccaaatt aattctaaag
    14761 gatttcaagg tgtcttttct atatgttttg gaaaataaaa aagatgctgg tgtcatccac
    14821 caacatcaaa aagtatagag ctagaaaaat atagaaggaa gctggacatt tattgttccc
    14881 agcttctttt ttgtatagac ctatttctat ttttttatta tattggatat atatattcat
    14941 tgaaaataga gcataacaac tttataatgt tgagtatacc aatttttgga ggcgttttaa
    15001 tggaccgcta tttagattta ctttcagaga agttccctac aacggaagat gtcatcacgg
    15061 aaatcatcaa tctcgaagca attttgcagc ttccaaaagg aacagagtta tttttgagcg
    15121 atatacatgg cgaatttcct gcttttgatc atattctgcg tatcggttca ggaaatctta
    15181 aagaaaagat caaagacctt ttctctgaac aaatgacgga aacggagatc agccaattca
    15241 ctatttttac ggcttatcca gaatatgtgt tgacgactga ttggtacaaa aaacaagata
    15301 aatcccagct gatccatcgt ttgatcgatc ttttacggtt tacgacagtc aaatacactc
    15361 gatcaaaagt tcgaaaagga ttgccgaaag aatatagcta tatcattgag gaactcttgt
    15421 atatcgatga tcgtatcaac gggaaaaaag actatgtaaa aaaaatcatt gaacaacttg
    15481 tcttgatgaa ggaagaagaa aaatttttaa caaaacttgc gcagacgatc caaaaatctg
    15541 tcatcgatca tttgcatatc gtgggtgata tttttgatcg aggaactcaa gcagccaaag
    15601 taatggatcg aatcatggat ttttcctctg tagatatcca gtgggggaat catgatattt
    15661 tgtggattgg tgcgtattgc ggctctgagg catgtttatt gacgctttta cggattgccg
    15721 cacgctacaa ctatttattt gaattagaga aagaatatgg attaaattta cgtccattat
    15781 tttcttttgc ccatcaaacc tatcagaaga atccagtatt tacccctaaa aacaggaaag
    15841 atctatctga aagagagcaa gaagaattag aaaagatcca tcaagcactt tctatcctcc
    15901 aattcaaatc agaacatcag ttgttgaatc gacgacctga atttaaaatg gacgatcgta
    15961 aattgttgga aaaaatcaac tatgaggaat caacgatcgt tttagaaggt cagctctatg
    16021 agttgaaagg gacttgtttt caaacgatcc agccagatga tcctaaaaaa ttcttggaag
    16081 aggaacaaaa agtgatcgat agtttgatgt attcattcca gcactcgtta agaatgcaaa
    16141 aacacatgac gtttttgatc gaaaaagggg gaatgtatct cacgaacaat cagcatgtac
    16201 tgttccatgg ctgtatacct gtagatgaaa aggggcagct cttagcattt gaattagacc
    16261 aatcttatcg cggaaaagaa ctgctgggat tttttgaaaa acagataact gaaagcgcaa
    16321 aagatttgac tgcaaatgaa gatttggcga ccgatttgat ttggtatgcg tggagcgggc
    16381 cattttcgcc tttattcggc aagagtaaaa tggcaacttt tgagcgctat tttctcacag
    16441 agcctcatac gcacgtcgaa aaaagcaatc cctattatca cttgcgagat gaagagtggt
    16501 tcagtgaaaa aattttggca gaattcgatg caaaagaaga aggttcagca atcatcaatg
    16561 gacacacgcc agtaaaagta aaaaaaggag agtcaccgat caaagcaaac ggaaaagcat
    16621 ttgttattga cggaggtcta tccaaagctt atcaaaaaac aaccgggatt gctggctact
    16681 cattactatg caattcttac ggtttccaaa tcgtgacaca ttatccgttc ttgcctattg
    16741 aacaacaatt tgcaaaaaaa tctgatcaaa cgaatgtaaa aaaagtgatc gagcagcagc
    16801 tcccacgcaa acttaatcgg aatacgacaa aaggaatgga attacagcaa cagattactc
    16861 aactccaaag gctgttggat tatcgaaagg acgattgaga aaaatgctag ccaacattgc
    16921 tcaatagact aaaaagcgac tgcaacaaaa ggcgtgtcgc agtctccttc ggaattagct
    16981 caaaaaacgg taaaatatta gaagctattt ttctgtttat ttctctaata ctcaggtcag
    17041 ggcgctgatt tcacgacctc ttccttaaac ggtgttctcc tgccaactcc tcggtcacaa
    17101 gtcacaatat ccaacaaaca tgagaagctg ttttttgaaa ttgctccttg tgactcagag
    17161 ttgaacggca ggctcacgac ctaatcagaa aaacgattaa aattcattca caaagaaatc
    17221 gaataaacgc tgttcagatg aatctacacg atgagaaagt tccggatgcc attggacacc
    17281 aaggattttg tgcatattat ctttactttg gactgcttca atgagtccat cgttggaaaa
    17341 ggcgatggct tttaacaaag gagaaagatc tttgactgcc tgatgatgat aggaattcac
    17401 aaagtatgaa tcagggagaa ggtcacttaa taaactgtcc gaaacgattt tgacttcgtg
    17461 agttgcaaac tgcggggcag tgggctgttg ttcgtgtttt acggaccatt ttggatataa
    17521 tgaaagatct tgataaagtg ttccctccaa cacgacattc aatagttgaa gtcctcggca
    17581 gactgcaaaa atcggttttc tttgtttcaa tgcttccaag atcaatgcct gttcgaattg
    17641 gtctcgttga atatttgttt ccgtcagttt tggatgaggg tcttccatgt aaagttgcgg
    17701 actaacatcc tgaccgcctg caagaatgag tttgtcgatt ttctcaatta gttgaggcgc
    17761 tgaatctgga gacatgactg gcagaataag aggtaatcct tgagcatcta aaactgcatc
    17821 cacaaatccc tgcggtgtat agcttacttg atttccttgg aaagtatctg tcgctcgaat
    17881 cagttgattc ccggcaatgc cgatgattgg tttcattttt attcccccta aagtagacta
    17941 attgtaacaa ggatttaaaa aaatttaaac tatgtaagac ttgcccctat acttatctgt
    18001 tcttgctgtg ttactatctg taaagagaat tttcgtgtga aattcatgac gatagttgaa
    18061 aatattgctt aatttgttat aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct
    18121 tcaaggatct agttcttcaa ggggtgagaa ggacatttta ttgaaggggc ctgcctatac
    18181 taagttatct ttagaggtac atactttcaa tgaaaaaaga aagcatgtac ctctttttta
    18241 aatcgaaagg aagtgaagca agtgttagaa gctttggaac aaatccgaaa agcagaagaa
    18301 gaaaatgaga agcgtgctcg aaccctacgg cagcagttga agacctatgc agaagaaaaa
    18361 gagcaagaat tggagataat ccaaacgaac agtcggcaac agctgaaaca gttggtagaa
    18421 gaaaaagaac aattcgaaaa gcaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa
    18481 gcagacaagg tgcaagcaac tttgaaagac caatatcaaa agcatcagag tcaagcaatc
    18541 ggtgcaatca ttgaaagggt gaaagaaact tatggccgtc actaagatgg aaaaagtgac
    18601 cctcatttca gataaaagaa atcaggagag gattttacaa gcagtacaag gaatccaaaa
    18661 tgttgaaatc cgggatctgt atcaaaatag aacaaacaac cagtgggtag aaaaatattt
    18721 tccagatacc acgattattg ataaagaagc aaaattaagc gctttaggca atcggctgaa
    18781 agacatccaa gaagctatcc aattcataca acaccacggc gatagccgtc aaaagaaact
    18841 ccatttgaaa cgtcaagaac tttccttaca agacttggaa gcaaattatt ccgaagaaaa
    18901 tttcatgaat aaattagaag aagtccttga tttaaaagaa aagtgggaac agctaactga
    18961 aaaacgtgat cgtttagaag aagaggaaga atggcttctt cattggcaac agctggatat
    19021 tgctccaagt atgtatgcaa gtaagacgac catttttgag atggggactg tgagtgcagc
    19081 aaattttgaa agtttcaagg aagaactaag taagcttgat gacgtttata tggaagaaat
    19141 agattatacg ccaaaacaag tttatctttc gtatgttgct ttaaaaaaat cagttgctgc
    19201 tatagaagaa attgctggga aatacgggtt caataaagaa acgtatcctt atgaacaaaa
    19261 tcctaaagaa aaattgcaag aaaccaaaca gcaattatcc attgttcacg agcagcaaaa
    19321 aaagataacg acagcgttag gtcagtgcag cggctatatc caagaatttg aatgggtaga
    19381 agaagtcaca cttgccgttg cagaacggga aaaagtaaaa gaacggttta tccatgccac
    19441 ttatctgatc gttttgcaag gttgggtaga tgcagaagaa aaacagaatt tgatgcatgt
    19501 gttaaacgaa actgtatcag aagaagatat atacgttagt ttcgaaacac caaactcaga
    19561 agagattcag caggaagtcc ctactaaatt gaaaaaccat ccgcttatcg aaccttttga
    19621 attgctgact gaaatgtaca gtctgccaaa atatgaagaa gtagatccaa cgccttggat
    19681 gacaccattt tatcttgtct tcttcgggat gatggtcgca gatgttggtt acggattgct
    19741 gatgctcatt gggacaatac tagctcaaaa actatttgtt ttaccccgcg ggatgaaacg
    19801 tttcgtaaaa ttctttgaga ttctctcggt accatcgatc atctggggat tgatctacag
    19861 ttcatttttc ggacaggcat taccaaaaga actatttggt atccgcttgc ctttcccaat
    19921 cctatctacg acggatgacg tcaatacgat tttgatttta tccgtgattt tcggtctgat
    19981 acagatcttg gttggattat tcgtggcagc aaaggaaaat atcaggagga aagattactt
    20041 gagtgctgtc agtgatggtt ttgcctggca agggatcctg attgggatcg ttattgcatt
    20101 gattggtgtc atgttgataa aaaatacagc atttgtttat cttggcgcgg ggctatcgat
    20161 cattgctgct ttatgtatct taatcgtgcc gattatccaa tcgccgtcta aagtcaaggg
    20221 tgctgccaaa ggagcgtata atctttacgg attgactggt tatatcggtg atttagtcag
    20281 ttatacacgt ttgatggcac taggtatttc aggcggaagt attggcgcgg cattcaatat
    20341 gctagttgct ttcatgccgc cagccgctcg attcagtgta gggatccttt tgatcatcgc
    20401 tttacacgca ctgaatatgt ttttgacact gttaagtgcc tatgtccatg gtgcacgatt
    20461 gcagtatgtc gaattttttg gaaaatttta tacaggaggc ggacgtgcct ttgacccgct
    20521 gaaaacggcg gaaaaatacg tcaacatcaa tcataagaaa aaaaacaata aataattgga
    20581 ggatttttat acaatgatgg attacttaat tagtcaaaat ggtggaatgg tatttgcagt
    20641 attagcgatg gcaacagcga cgattttttc agggattggt tcagctaaag gtgttggaat
    20701 gacaggtgag gcagcagcag cgttaacaac aagtcaaccc gaaaaattcg gacaggcttt
    20761 gattctgcaa ttgcttccag gtacgcaagg gttgtatgga ttcgttatcg cctttttgat
    20821 ttttatcaac ttaggaaatg atatgtcagt tgtacaagga ttgaactttc taggcgcttc
    20881 tctgccgatt gcttttacag gtttgttttc aggtatcgcc caagggaaag ttgcagcagc
    20941 aggtatccaa attttagcga aaaaacctga acatgctaca aaaggaatca tttttgctgc
    21001 gatggttgaa acatatgcga ttttaggttt cgttatttcc ttcctattag tattgaatgc
    21061 ctaagcataa aggaggaaaa gcagatgaac gccattgaaa aaatcatctc tcagatgaat
    21121 gaagcagcag agcaagaaag agcggcgtta gagcaagaag agcgaatgaa gatcgatcaa
    21181 aactttgaac aaaaacgtac gcaaatcgaa acagaacatc aaaaacaaaa agaaaaacaa
    21241 atcgaattac ttgaaaaaaa ataccgtcag cttaggaatc gccaacaagt agaagtacga
    21301 caagaaaatt tgaatgcgaa gcaggaattt ttacgacgat tgtttgcaga tgcaatagct
    21361 gaaatggaga attgggatga atctgaacaa atccagttta tcaaaaattc attgtattca
    21421 ttacctttga caggcaaagt aacattcatt gctggagaaa aatcagcagc ttatctttct
    21481 cagacattgc tggatgaatg gaacaatgaa cttcccttta tgatggtttt aagtgatgaa
    21541 actgttgctg atcaagcggg ttttctgata aacgatcaag gagttcagta taattttctt
    21601 ttcagcagcc ttgtacaaga tatccaagga acgatgagtt ttgagattgc caatcaatta
    21661 ttcgaataag gagggatcag tgtatggaat accacgaact aaatcccttg attcgcggaa
    21721 gagagcttga acttttgtcc aatgagacat ttgaacggat gattcaaaca aattctgtcg
    21781 aagcacttgg tgaaatattg aaatccacga tttacagtcc atatatttat gacggatatg
    21841 aatatgactt cgaacagaat cttttaaagg agcagagccg tctatttcgc tggttaaagg
    21901 aatcagcacc ggagcctgaa atcgtttgga tctatacgat gcgctatact ttccataatc
    21961 tgaaggtttt gacaaaagcc gagatgacaa atcaaaatct ggaccatttg tatatcgacg
    22021 atggatttta ttcattagaa accttgaagg atgccattca tacgcaagtt tcaaatgaac
    22081 tccctgaaag cattatggac agtatacgag aagtacgtga ttattttgaa gagtctacca
    22141 ttcttcaggg aattgacgtg atttatgatc gacagttttt gacagagcag cgacggcttg
    22201 ccgataagct aggatatcaa gaattgctga cagaagtcat tgcttttatt gacttgacca
    22261 atatcacaac gatggctcga ggaatattgc agaaaagaac atatgggttc atgacgacgg
    22321 tactttccag ctcgggaagt atcgaaaaag aagtattttt gaactttgta gataaagata
    22381 tggaagatta cacgaatttt ctattagaga cagattatgc tgattttctg cgccctgtca
    22441 tccgtgaggg aaatcttgat ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt
    22501 cattgtatca agaagcacaa acccaggctt tcggaccact gccactacta gcctttttga
    22561 acgcaaaaga ggtagaaagc aaaaaccttc gtttactggt tatcggcaaa agaagccatt
    22621 tcgaaccaga aacaatcaga aagcgggtga gacaagtcta tgacgcataa gatcggtgta
    22681 gttggcgaca aagattctgt tttgccattc aaattgtttg gctttgatgt tcgttatggt
    22741 acaaccaagc aaaccgttcg tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg
    22801 atttatgtca cagagcaatg tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa
    22861 caaatgatac ctgctatcgt attgattccc aatcatcaag gatcattagg gatagggctg
    22921 gaagaaattc aaaaaaacgt agaaaaagca gtaggtcaaa atatcttata gtatgaaaag
    22981 gagatagaaa ttttgcaaat tggaaaaatc ataaaagtct ccggacctct tgtaatggct
    23041 gagaacatgt ctgatgccag tatccaagac atgtgtctag tgggagatct aggagttatc
    23101 ggagaaatta tcgagatgcg tggcgatgtg gcgtctattc aagtatatga agagacttct
    23161 ggaattggtc caggcgaacc ggttcgctct actggtgaag ctttatctgt tgaattagga
    23221 ccagggatca tttcacaaat gtttgacgga atccaacggc cattagatac ctttatggaa
    23281 atcacacaaa gtaatttctt agggcgcgga gttcaattgc ccgcattgga ccatgagaaa
    23341 aaatggtggt tcgaaccaac agtagaagcc ggagaaacgg tgagtgctgg agatgttatc
    23401 gggatcgtag aagaaacaaa agttataaaa cataaaatca tggtgccaaa tgggataaaa
    23461 ggaacgatca aaacaatcga aaaaggttca ttcactattg atgaaacagt atgtgtcatt
    23521 gaaaccgaaa atggtgacaa aaatttgacg atgcttcaaa aatggccagt aagacgtagt
    23581 cggccaatca aagaaaaatt aaacccggat gcacccatga tcactggaca gcgagtgatt
    23641 gatacattct tcccagtgac aaaaggagga gcagcagctg tacctggtcc ttttggtgca
    23701 gggaaaacag tcgttcagca tcaaattgca aaatgggccg atgtggacat ggttgtctat
    23761 gtgggatgtg gcgagcgtgg aaatgagatg acggatgttt taaatgaatt ccctgaattg
    23821 attgacccta atacaggaga atcgttgatg gaacgtacgg tattgattgc taatacatca
    23881 aacatgccag tagcagctcg agaagcttct atctatacgg gaatcacaat tgcggaatat
    23941 ttccgtgaca tgggttatga cgttgctatc atggcagact ccacttcacg atgggcagaa
    24001 gcacttcgtg agatgagcgg acgtttggaa gaaatgcctg gtgatgaagg ttatccagct
    24061 tatcttggtt cacggcttgc ggaatactat gaacgatctg gaagagtcat tgcattagga
    24121 tcagaaggtc gcgaaggaag tataaccgca atcagtgctg tatccccgtc tggaggggat
    24181 atttcggaac cagtcacaca aaatacattg cgggtcgtaa aagtattctg gggtcttgat
    24241 tccagtttag cccaaaaaag acattttcct tcgatcgatt ggatacaaag ctattctctt
    24301 tattcaacag aagtagggaa gtatatggat catatccttc aagctgactg ggcatccatg
    24361 gtcaccgagg gcatgcgtat tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg
    24421 gtagggattg attccctttc agataatgac cgtctgacat tagaagtggc aaaatccatt
    24481 cgtgaagatt atttgcagca aaatgctttt gatgatgtcg atacattcac ttcaagagag
    24541 aaacaattca aaatgttgaa gatgatcttg acctttggtg aagaaggacg ccgtgcgttg
    24601 tcgttaggtg cttatttaag tgagatcatg gacggtaccg tatcgattcg tgaaaggatc
    24661 ggacgaagca aatatatccc ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa
    24721 atcaagcaaa cgatgcaaac gatcgtttca gaaggaggca tgagcaatga ttaaagaata
    24781 tcgtacgatc ggtgaagtcg tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa
    24841 atatgaagaa ttaatcgaag tccgtatgca aaatggcgag attcgtcgtg gacaagtatt
    24901 ggaagtccaa gaagataaag caatggtcca aatattcgaa ggaacaagtg gtatcaattt
    24961 acgtgactct tctgtacgat ttttaggcca tcctttagaa ctaggtgtat cggaagacat
    25021 gatcggtcga gtattcgatg gtttgggacg accgaaagat aacggaccag agatcctgcc
    25081 tgaaaagcat ttggatatta acggagaagt aatcaatcca gtagctcggg attatccaga
    25141 tgagtttatc caaacaggta tctctgcaat cgatcatttg aatacattag ttcgtgggca
    25201 aaaattacca gtattttctg gctctggtct tcctcataaa gaattagctg ctcagatcgc
    25261 gcgacaagca acggtactcg attcatcaga tgatttcgcc gtagtctttg ctgctattgg
    25321 aatcactttt gaagaagctg aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga
    25381 ccgttctgtc atgtttatga atttagcaaa cgaccctgca atcgaacgta tcgctactcc
    25441 tcgtatggct ttgactgctg cagaatatct tgcttatgaa aagggaatgc atgttttggt
    25501 tatcatgaca gacatgacca actatgcaga ggcattgcga gagatttctg cagcacggcg
    25561 agaagttcca ggaagaagag gatatcctgg gtatctttac acgaacttag ctactttatt
    25621 cgaacgagca ggaagaatcc gcggactaaa aggatcggtt actcaaattc cgattctaac
    25681 aatgccagaa gacgataaaa ctcatccaat ccctgacttg actggttata ttacagaagg
    25741 acagatcatc ttgactagag agctttacaa gagtgggatt cagccaccaa ttgatgtact
    25801 cccgtctctt tcgcggttaa aagataaggg aactggaccc ggaaagacaa gagaagacca
    25861 tgcagctacg atgaatcagc tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt
    25921 agccgtagtt ttaggtgaat ctgctttatc tgatgtagac aagatctatg cgaagtttgc
    25981 tgagcggttt gaaaaagaat atgtcaatca aggattctac acgaaccgtt cgattactga
    26041 aacacttgat ctagggtggg aactgctatc gatgttgcca agaaccgaat taaaacgaat
    26101 caaagacgac atgctggaca aatatctgcc agaagggaag tgagttctga tggcacgatt
    26161 gaatgtgaat ccgacccgca tggaattgac tcgattgaaa aaacagttga caactgccac
    26221 aagagggcat aaattattaa aagataagca agatgaactg atgcgtcaat tcatcctgtt
    26281 gattcgaaag aacaaccagc tgcgtcaaga aatggaagag tctatgacag aagcaatgtc
    26341 tgattttgta ttggcaaatg cttcgatcac ggaacctttc atcgaagaat tatttgtttt
    26401 gccggcggaa aacgttgatt tgtctatcgt agacaaaaat atcatgagtg taaaagtacc
    26461 tgtgatgaat tttgaatatg atgaacagct gacagacaca cctttggaat acgggtattt
    26521 gaattccagt gccgaactgg atcggtcaat cgatcggttt actaccctgc ttccaaaact
    26581 tttggaactg acagaaatcg aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac
    26641 acgtcggcga gtcaatgcat tagaatatat gacgatccct caattagaag aaacgatcta
    26701 ttatattcgt atgaagctgg aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt
    26761 aaaaaatatg ggtaccactg actaagtggt atcttttttt aaaagaggaa aacgatgatt
    26821 aaacaaagca aacaaataaa gaaacgtttg ccggctgttc agcttctcgc attaggattt
    26881 tttctcctga ttttagttgg cggcggccta cttaccctac cattttttag taatagtggg
    26941 catgggacaa attttatcga tgcgcttttc actgcaactt ctgctgtctg tgtaacggga
    27001 ttgacaacat tgaatacagc agagcattgg aacagtgctg gtcaattttt gatcatgctt
    27061 ttgattgaga tcggcggtct tggttttatg atggttccga ttctatgctt tgcattgata
    27121 aagaagaaag tcagtctcag cacacgtatt atattaaaag aagcgctgaa tctagaagaa
    27181 gtatcaggtg tgatgaagct catgctttac atcctaaaat tggcctttgt tatccaattg
    27241 attggtacgg ttgctttatc ttttgtattt gtgcctgaat ttggctggtt gaaaggtatc
    27301 tggtacagtt tgttccatgc aatctctagt ttttgtaatg cagggtttga tttgttagga
    27361 gacagtttag ccaatcacca gcaaaatgtt tatttgatca tggtggtatc tgctttgatc
    27421 atttctggtg ggttaggctt catcgtatgg cgtgatttac tggaatatca tcagaaaaag
    27481 aagatgacga ttcattcaaa aatcgcatta accgttacag gtaccctttt attaggcgga
    27541 ttcgttgtgt tctttttcac agagcgcaat ggggcaacgc tagtggaagg gaatatgttt
    27601 caacgattgg caaacacctt ttttatgagt gtgacaccta gaacggctgg atattattca
    27661 gttgattacc tccatatgag ccatgcaggt ctgattttaa cgatgttttt gatgtacatc
    27721 ggcggaacat ctgggtcgac ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg
    27781 atccagatgc gttcgatgtt gaaaggacgt acgagagcgg aagcatttgg ccgtaccatc
    27841 cgacaaggcg cagtgttgcg ggcacttact ttattttttg ttacgttgac gttgtgtgta
    27901 gtcgccatca tgattttatc ggtgactgaa acgataccta aaacttctgg catcgaatat
    27961 attgcttttg aggtcttctc tgcgtttggt acagtaggtc ttacgatggg attgacgcca
    28021 gacttgactg caatagggaa attgatcatt atagcgctta tgtacattgg acgagttggt
    28081 gtattgactg tggtcttctc tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa
    28141 tatccagaag aaagtgtcat gattggataa tttcatcttc tgacagaaac ctagtgattt
    28201 agtaaaataa gaaaataaaa taatttttct tgcttcttgg atactttaag tgcaaaatgt
    28261 tcgtttattt tttgaggcac tacccgttgt tgaacctcgg gtagtgcctc ttttttattg
    28321 aaaggaatca ataagatttg attcctttat ttatcaatag gttataattc atattttttt
    28381 attttttctt ttacgatact tagtccagaa gctaaaattt caaggttatt agaggatagg
    28441 gaaattcgaa cgaatgcatc tggttgcgca cgtgtagtaa agcgtctgga atgataaagt
    28501 cggataccgt tagcaagaaa gtcattctca attatcttag ggtcaatctc aggatcaact
    28561 ggaagaattc gatagtaagg gacacgattc gttggttttt ctactttgaa aagagaatca
    28621 aaaagctgat tggctctttt tgtaagactt aatttagttt ctaagatcct atttgcttca
    28681 tttgaatgga ggatttgagt gatgacttcc acatctaatc cagaagtttt cacatttata
    28741 ttaaaaattg cttgttctat cttgctttgg acatgtactg gaactacaag ataagcaact
    28801 cgcaatccag aacaaataaa ttttgtcatt ccagctaagt agattgtttg gttaggtaat
    28861 aaattttgga aaggaggaat gatcgtatgt tgatagtaag ttgttaaaaa tgaatggata
    28921 tcatcttcaa taacccatag accttctttt tcaatcactt cggctaattg ctctcgtctt
    28981 ttgtgagaga tttgaaaccc tataggatta ttacaagaag gcataagaaa gacaccatga
    29041 atcttctttt ttctacattc ctgaatcaat atatcaggtc gcattccttg ctcgtcaaaa
    29101 tcaattggaa cgatctctaa atgatataac tgagccaatt caatgaaatt agcgtatgta
    29161 tatcggtctg ttgctattct atcacctgga gagaaaagtg cagaaagtgt gatagccaat
    29221 ccattttgaa caccggaaac aatagataga gattctaagg acggaatgac tccttgagtt
    29281 tttaaccaat ttgctgcagt agtcaattga aaatttgtgc ctttgggatc ttggtaatta
    29341 agcaaaccaa caagatttgg gttattacta gttgattgaa taaacgggac aacgaattta
    29401 ttacattctt caaaagaact aaccagacca aaatcaatga tatgttcggt tacttcattg
    29461 gtagagatgg tgatggactc aaagacattg ggagaaataa atgtgccact tccaatattc
    29521 gtatacagca aacctttctc aattccatat ttataagcct tgccaacggt agtaaaattt
    29581 aaatctaaat aatcagctag ctctctttgg gagggtaact gagtgttttt ctgtaattta
    29641 ttctttaaaa tatcttgttc taattgttga atcaacgatc gataataagg acgcttcaat
    29701 ttttgtttgt ctggtcgcca agttaacggg taattgtcat aagaattaat aggcattttc
    29761 tccactcctt aaaattgtat accatacaat tttaccattg attgtattga ttttagaagt
    29821 taatcttttg tgaggaggga attgtctatg aaaattatta attttgagga agtagtagct
    29881 tcttttagtt ttgaagaagc gattcaagta atgaaaaaat gtttttttga ttatgaaagt
    29941 ggtaaaatca gccaaaatcc tcgattggtg gaaattttac ctgacggaaa aggcaaaaat
    30001 atgtttgcga tgatgcctgc ttatttgggg gagaacagat attttggcac aaaagtcatt
    30061 accgcttttc cggataatca tcagtatcat ttaccttctc atttaggaga gattttcttg
    30121 tttgatgctc aaaacggact acccaaagca ttgataaacg caaatgcagt cacctggatt
    30181 cgaacagctg ctgtatctgc gctggcaaca gattacttag caaatccgca tgcagaaaca
    30241 cttgctttaa ttggagctgg tcaacaagca ttgtcgcatt tgactgcgat ttctaccatt
    30301 cgtcccatca ctacagtgtt tgtttacgat attttagaag aaagacgaaa tacttttatc
    30361 gctaaagcta aaaaacagta tcctaaaata acgtttataa atgcaatatc agtagaagat
    30421 gcagtacgtc cagcggaaat catttgtacg ttgacctcta gtaaagaggc atttcttcaa
    30481 aaagagtggg taagggaaga cgcacatatc aatgctgtgg gcacatttac tcctgataca
    30541 agagagataa gtacatcgtt gatggaaact tctcaaatct atgtagatga ttactcagct
    30601 gccttaacag aaagcgggga cctgcttata gccatggcag aaggagattt agaggaatct
    30661 tgtattgttg gtacattagg cgaattagtt tctgagaaaa aaacatttta taaaggagaa
    30721 tcgaggccga cgattttcga cgcagttggt ctagctattg aagatctttg ttgtgcagaa
    30781 tatatttata acaaattaga gaaaggggct ttaaaatgaa cttagattat tacgtagttg
    30841 atgcatttgc agatgaggtg ttcaaaggaa atccagcagc ggtttatgtg ctagaagaat
    30901 ggctgccaga aggtacgatg caaaaaattg ccattgaaaa caatttatct gaaacagcat
    30961 ttacagtgaa gaaaaaccaa gaatttgagc ttagatggtt tacgccagat cgagaaatcg
    31021 atctttgtgg tcacgcgaca ttagcaacag catttgtttt atttaactat tacaaaatac
    31081 ctgatgaaac gattaaattt tctactcaaa gcggtaatct atttgtgaca agaattaaag
    31141 attattatta catggatttt cctagtatta tgccgaaaaa agtgccgata ttgtctgaat
    31201 atgaagaagc gattggggct aagattaaag aagcctactt agcacgtgat ttatttttcg
    31261 tattagaaga tgaaaaaacg gtggctaaat tgcagccaga ttttacagct atcaaaaatt
    31321 ttgaattagg aataggagtc atcgtgacag cagaaagtgt gcaaaaagac tttgtttcta
    31381 gaaccttctt tccaaaatta acaatcaatg aagatccagt atgtggatca gcacattcta
    31441 atcttattcc ttattgggca gaaagaatga ataaaacaaa attgtcagct tatcaaatgt
    31501 catcaagggg cggggatcta gaatgtgaat taaaagaaga tcgtgtgata ataggaggaa
    31561 aagcagtttt gttttctaaa ggtacctcgt ttatttgatt tctttcaaat gaattaaaaa
    31621 taaattggtg gagagtggca accttatagc tacaatggtt gttaaaaaaa gacgcaaact
    31681 ttttcaattt aatgaaaagt ttgcgtctca ttttaattat tggtgtttgt tttttattca
    31741 atcaagagta aaatgcccca tactaactat cctcggatga cttcgatttt ataaccatca
    31801 gggtcaatga caaagtagta agaaggcgca gtgccaggta atccttttaa gtcagtaatc
    31861 tcaagaccgg cagctttatg ttgctcgtgt aatttttcta gatcatcagc tgctatagcg
    31921 atatgaccat agccgtctcc tagatcatac gcaccatgat catagttgta agtcaactct
    31981 aattcatagt catccccagg aagtgtcaaa taaacaagtg taaatttgtt ttctggaaaa
    32041 tcacggcggc ggctttcttc aaaaccaaat gccttttgat aaaattcgat tgatgcttca
    32101 agatctttca ctcgaacaca tgtgtgagcc attttcatag attcatctct ccttttcctt
    32161 ttactcaatc ataacaaaaa gaagtaagga aatagttgat ttgtgcttgt tcatgagaaa
    32221 tttctcatgt aaaatggata gaaaataaaa ctgttaaggt ggaaactatt catggataaa
    32281 ccaatttttg gtgaaaaaaa gcaatttgct gattatcaaa cccgttatgc agcatatatt
    32341 gtcatagaaa gagatcagca gattgcagta atcgaggctc caaatggtgc tttttttctt
    32401 cctggcgggg aaattgaagg agaagagacg aaagaagaag cgattgtccg tgagctgata
    32461 gaagaaatgg gaattgtggc agaaatttct tcttatctgg gagaagctga tgagtatttt
    32521 tactcgaatt atcgtgcaac ttattattat aatccaggat atttctttgt tgctgattct
    32581 tggaagcgta taggagaacc aactgaaaag accaataaaa tttggtgggt gagccctcaa
    32641 gaagcgcttg tgaaattgaa acgtggtagc caccgatggg cagtcgagag atggatcgaa
    32701 aaaaataaaa actgaaagaa agcgagtaga tcagctttct ctcagttttt ttatttatta
    32761 cagtacatat tttttgattg cttcagctac accgtgctcg tcgtttgatg aagtgatgac
    32821 atttgctgct tctttgacaa gagaaacggc atttcccatt gctactccta gaccagcata
    32881 ttcaatcatg gataagtcgt tctcgttatc tccgatagcc atgatttcat cttgatcaat
    32941 accaaggtgc tgtgcgagat tcgcaactgc cgcaccttta cttgcttctt tatttagtac
    33001 ttcgaagtaa tagggcgcac ttttgaccgt tgtgtattta tcacggaaag tttgcggtaa
    33061 acgcgcaatt gctgcatcta aaatttctgg ttcgtcgatc atcatcattt tgacgatgtt
    33121 catgtctggt gtcatttctt caggtgttcg atattttagc ggcatcttga ctaaacttgc
    33181 ttcgtggatc gtataagcgc tgatatctcg attaggcgtg tagatcgctt tgtcatcgat
    33241 cgaatggaga tgactgccga cacggcgtgc catcacttcg atctctagga aatcttcatg
    33301 tgtcagtccg tgacgggcga tgattttacc tgatttcgtt tgttgtacga gtgccccgtt
    33361 ataagtaata acgtaatcat tatcttgaaa aaggttcaac tcgtttaact gttcctggac
    33421 acctggtaag ggacggccag tgcataaaac gatcgaaaca ccttttgcaa cagcagcggc
    33481 aatcgcttct tttacttctt ttgttacttc tcttttctca tttaataaag taccatcgat
    33541 gtcgatagcg actagtttga tagacataat ttcctccaat atattttaat ttatttcaat
    33601 caatgcgcca ttacggatat ggctggcaaa ttcttgataa gtttcgtcaa ataaatcata
    33661 atgatcacgc aaggacggat caaccatttc ttttggaaag aaaaatcgct catctccttg
    33721 ttcttgtccc gctaatgcag caacaagctg actaacctgt gataattcta tcaaagttcc
    33781 gtcatttcgc aaaatctcga tttgtgtgcg atgtctgcct tgttctgggc gataaaaatc
    33841 ataaggtaga tcgtagctag aattcacagc agtgtaataa acaggattat atccgacctt
    33901 ttctactaac aaagtgagtt cttggactaa ttttgaatcc cggttgccag aaaaagtagc
    33961 agatttcagt ggtttacgat tcaaaaaacg tttagctaaa tcatttaaaa taggatcagc
    34021 agattcagac cattgagtaa agtaagtgcc caagacccca tcatcaagtt tgaggtattc
    34081 ttctaaagta aagcttccgt caagaaatgg cagaagtaat tgagaatgga gttcaaaact
    34141 gctggcatct tcttgataca actcgtgtgc gcgatgaagc agatgctcca aaattacttc
    34201 cataccacgg gaaacaggat ggaagtacac ttgcacatac atttgatatc ggctaacgat
    34261 ataatcttcc actgcatgca tgccactcat ggaaaaagcg atcccgcctt cataaggacg
    34321 aatcacacgt aatattcggg taagatcaaa tgttccatat tctgttccag tgaaataagc
    34381 atcccgaagc aaatagtcca ttcgatctgc atcgatctga ctagaaatca tttggacaac
    34441 ttgtgggttg gggtaagttt tttggatcac actagctacc ttttcaggaa aaccttcttc
    34501 tactcggtta agaatctgat acacttctgt ttctggagaa gtgatgatct ctacagtgat
    34561 ggcttcatga ttcgtatgga agatatgttc aaatgtatga gagtaaggac catgaccgac
    34621 atcatgcaac agtgccgcac ataaagtcac cagccgttcc tgatcatccc agcctccgtt
    34681 gcctattttt tcttttgaaa aatttcgaga aaagatatcg cagattcgtc ttgaaatctc
    34741 ataaacgcct aatgaatgag taaaacggct atgttctgct ccgtgaaaag tgaaagaaga
    34801 agtgcctaat tgtttaatgc gtcgtagacg ttggacttct ttcgaattga tcaaatctaa
    34861 gattacttga tgctggacat ggatatagtt atggactgga tcacgaaaaa ctttttccat
    34921 aggcagcatt tgatatttat aaggggttgt cataatgtag ctcctttatg ttattacgtt
    34981 gttccattct ctggatttgg ttgttccatt gctccagctg gatttctttt tttagccagt
    35041 cggtagaatc ctgtaaatca cttgcagaat acagcttttc aaaagctttt gcaaatgctt
    35101 ctttgacttc ttttacaaaa aggggttggt caagcagttc ttcaagtgtt gccattgtag
    35161 caggatccac tgcaggatag ccatttgtac caaagtcttc ttttaaagca gattgataaa
    35221 atcgcttcat gacttgcccg cgtttttcct gattaccgtt gacacttaag tacatcataa
    35281 cagaaatccc atttttgatc cgacgctgag cgataccggc aaattttttg ccatggatgc
    35341 ttaaatcata agttccagga caataagaat gggtgatttc gaatgcttgt atcggtgcct
    35401 ttggaaaagc gtatttagtc aataggagca tttgctcgta agcgtcatct atggttattt
    35461 tttttgtgtc actttggggg aaaatcaaag aaatattcaa aattccttca tctgatacga
    35521 tccctaatcc acctgaattt ctgatcactg gagcgtaacc tgcatctttc agtgtttgga
    35581 tcccgtcact tagaaaagga gttcgaacat ccttcatacc taagatgact gtgcagtcat
    35641 attgccagaa atgtaaaaag atttgctggt gttctttaga atattccgtt aatatatctg
    35701 ttaatgcaaa aggggaaaag tcgttttttt gataaatgtg ctgatctagt cgaatcgcag
    35761 ggttcattca accggctcct ttgtataaat tcatattttc tacaatatta tactctaaaa
    35821 tcaaagagtt gttttggaaa aaaggttttc tgtaaaaatg gacaagttgg ttgacaaaac
    35881 aaaagaaact aggaacaatg tagctatgag aagtaaagga gaatgaataa tggaattatc
    35941 acaaggaaca ccgatttcaa tcaaattgcg tacaaaagta aaacagcaag gggaagtgca
    36001 agatttttat tttgatttga aagggcaaat ggtgacaatt ggcgatacgc tgtacatacg
    36061 ttataaagag attcaggagg agacgggcga tgagattcct gtaacgatca aattagttcc
    36121 agatggtcat gtacagctga tccgtgcagg agaaatgcgg atgcgtttga aatttggcta
    36181 taaagaacgg ttagaaacat cctatcgaac accatacggt atgatccaga ttgctacttt
    36241 tacgaaagaa ctgcatgtta gtttgaaaga ccgtcccaca ggagggaagg ttcgaatcga
    36301 ttatgatctg ttcatgggac cggaaaaaat cggcgaatat tatttgacct tggattttac
    36361 agcgtaaata taaaagaaaa gtttgatttt ttgtgtctga ttttgtatga taaggagtag
    36421 actgtgaaag gacgtgtcct gattggaaat tagtgtattt gaaggtgcaa acaaaagcga
    36481 attatcaatg atcgaagtag cacacgcgat cttagaacaa cgtggcgatg tcatggattt
    36541 ttctgattta gctaaccaga tccaaaacta tcttgggaaa tcagacagtg aaattcgtga
    36601 ttcactcgca caattttata ctgacttgaa tattgacggc agctttatct cattagggga
    36661 taatcgttgg ggcttgcgtt catggtatgc tatcgattcg atcgatgaag aagtaaacca
    36721 tggaatggac gaagaggatg aagatactcc acgtcgccgt aaacgcaaga aagtcaacgc
    36781 ctttatcaat gatgatgaag acgcgatcga ttacaatgac gatgacccag aagatacaga
    36841 tctgacagaa gaagatgacg atgatctgtt tgatgacgat gatgatgaag atgaagaaat
    36901 cgctgcttat aattctgact tgcaagaaat cggagcagat gacccgacag atgatgaaga
    36961 agacttgcca ggaaatatcg aagaagattt aagtatcata gacgaagacg atgatgatga
    37021 agattatgaa gaggaagaat tttctgactc tgaaaaagaa taaacattat tttcttacga
    37081 gaggttgtga cagagacgat cagcctatca gaataaggag gtgtgacacg atttttgtca
    37141 caccttcttt tttgtatata aaaatcccaa gtaaatacat aaaaaataaa aaaattgaaa
    37201 cttttagcgc accttgtacg tcatattaag tgaggcagaa aggagtggag aatgattttt
    37261 actggatata agaaaaaacg gcagatcgaa aaataccgtc acctgctgaa tggactcatc
    37321 gaaactgatt atcaaaaaat gttccgtatc acgatgaatt acgtacatga taaagaagag
    37381 gccttagatg tgatgcaaga tagttttcat aaagccctga actattttga aaaagaaaaa
    37441 gagatcgagc attttagtgc atggttttat cggatattga tccgcacggc attagatagt
    37501 tggcgcagac aaaaaaggac gcctgtggac ttatttgaaa ttcaagatat cccccaacag
    37561 ttacctttag aaacttccgt tgcagagctc catatgattt tagataaaat agacagtccg
    37621 gaaaaagaga ttctgatcct ctactttttt gaaggatttc gtttgaaaga aattgcgact
    37681 atcttggata tgaatgaaaa cacagttaaa acgaaaatgt accgctcatt gaatgcttta
    37741 cgtcaaatcc tagaatgaag gagagaagag tgccatgtat tcccaaaaag aaaatttaaa
    37801 aaaactaaag aaaacttata aagaacaagc aattcccaaa gggcttaaag aggagatccg
    37861 ccagcgtttt attatcgaag agcaatcatt tataaaagca aagcggagaa agagacgtct
    37921 ccaagggttc ggagtatctt tggctatcgt aatgattaca acaagtagcc ttctttttaa
    37981 tagtcaagtt cgaagctttg cagaagattt acccatctta ggatcagtca ttgaattgat
    38041 cttaggcgaa cgctttactg atcgatctga aaaaatcgat attcaggtac cgaagatcaa
    38101 tacacaaaat gagaaagaaa ataaaacaat tcatggatta aatcaaaaat actttcgcga
    38161 aggacaagca gattttgaaa aagctgaaaa agaatatgga aattttgaaa cggatcatta
    38221 ccaagttttg ggagattatc agaaaatcgt agacgataac cggtttctcg tgattgaaag
    38281 gcagatcacc caaacagcag cagatagcca tgttgaaaaa agatacgaca caatcgacaa
    38341 gaaaaacagt gtccagctct cacttccact tttattcaaa gatgacacct atctatctgt
    38401 tttgacaaaa gaagtcaaac ggcagatggc cgaacaagtc aaggaagatc catctaagta
    38461 ctattggact gagcaagata ttcaagaagg gacgatagaa aaaccaacac ttgttacgcc
    38521 tacacgaagt ttttatttga acaaagagca tcaattagtt ttgacttttt ctcaatacga
    38581 aattgctcct ggttatatgg gtacaccaga atttgtcatt cctaagtccg tgacaaaaac
    38641 gattttggca tcggaagatt atttggatca ttagacgaga agtagttgcg actagcagtt
    38701 tcaattttca agtaagtaca agtaaataat aagaagagaa aaagagaaag aagagatata
    38761 gtgaaaagaa attatttagt tatcagtatt attgctttat ccgtgctttt gtttttcgaa
    38821 attacttcgg tggcctcctt gatcaccgaa gtaaaaggac aaacacactc ttcggagcat
    38881 tcgtcacaat ctttcaaaca atcagaacaa tcaaccatta aaagccaatc aactgaaaca
    38941 gctaacgtca aagaagccat tcaagaatca gcggaaaagg tacaaagtca gtcatcacaa
    39001 tcaacacaag acagtacaaa aacgagccga gatccggctg tcaaaagcgt cgcaatcagc
    39061 tttgatgatg gaccaggagc gaccacaaca ccgcagcttc tgcgaatctt aaaagaaaaa
    39121 aacgtgcatg ccactttttt cgtcttagga gaaaatacgg cgcagcatcc tgaaatcgtg
    39181 aagcagacag ctgaagcagg gcacgagatt ggcaattaca catacgatca tcagaatctg
    39241 gccactcttt ctgcccagtc gatgacggag gaagtgacga aagcagacac tgagatcaaa
    39301 aaagtcactg gcaaaacccc cacttttgtt cgtccgccct atggttctgt cacgaatgta
    39361 ggagcaacga tcatccagcg accgatcatc gaatggtctg ttgattcaga agattggaag
    39421 acaagaaatc ctgatttgat tttgcagaag atccaagcaa cagtatacga tggggcaatt
    39481 attttattcc atgatatcta tcctgaaacg attcgtgccg ttcctcaagt gatcgattat
    39541 ctgaaagagc aagggtatcg catcacgaca gttggggatt tattagggca tccaacggct
    39601 gtcgagaact attatggcag aaacgatcat cggccagttc agtaagtgag acaagtatct
    39661 ttaataaata acacatttta ataagtagtt gaacacataa ctaccttact tgcaaaggct
    39721 taacaatatc tatacaggta caacaaaaaa ctgtccacca attttctttg gcggacagtt
    39781 ttcacttaaa aataaagaag caactattcg taaattatgc taaaatagca gtagaacgcg
    39841 tcctgaggga atcgaacccc cgtctcaaga accggaatct tacgtgatat ccactacact
    39901 aaggacgcaa gcactgggat aattgtaact aaacagataa aaaattacaa ggggattgga
    39961 caaaaaaatg aaatttgagc aaaatttttc acaaaaacag cagcagacac agaaactagc
    40021 catgacgcaa cagttgcaac agtccatcca agttttacaa tataacagtg aggagctgtt
    40081 ggcattcgtt gagaatcaag caatggaaaa tccattggtg gaagttgttg agccggaatg
    40141 gcagccagat tacataaaag cttcttcatc ttcttatgaa ggagaagaga caaattacct
    40201 aaatcagatt cctgatacaa aagggtcttt atttgattca ttgatcgaac aagtccattt
    40261 gaattaccga gatacttttt tgaggaaaat cgttctttat cttgtagaat acatcgattt
    40321 gaacggattt ttgacgatta gtttagaaga agccggaaaa gagattggag ctacgcctat
    40381 ccaaatgctc gatgcgttga ctctgattca gcaattagaa ccagctggag taggtgcacg
    40441 tagtttgcaa gaatgtctca tgcttcaaac agaacgagat gattatgcgc cggaattagc
    40501 ttatatcgtg ttggaagaat gttttgagga actggtggag cgaaaatgga aagaaatcgc
    40561 acaacgtttt tctgttgatc tacatgctgt tcagcagata ttcgactatt tacaaacgtt
    40621 gagtccaagc cctggccgga ttttcgaccg tagcagcgaa ttgtacatta ttcctgatgt
    40681 ccgggtatta gtggacgacg ataaaaatgt acaagttatc tcaaaccgaa aaaatcagcc
    40741 aaatatacgc tttcaagaga gttacttcaa acaaatgtca caacaagcag ataaagaaac
    40801 tgaaaattat ttaaaagaac ggaaacagga attcgaatgg ttgaagaaaa cgatcctaca
    40861 acgtggggat acaattttgc gggttgctca agtcattgtt tcccggcaaa aagctttctt
    40921 tatcgacaag gaacgtccaa tcaagcctct tacgttaaag gaagttgcaa ctgagataga
    40981 tgtgcatgaa tccacggtca gtcgtgcagt caacgggaaa tatttggaaa cagatttcgg
    41041 tgtatttgaa ttaaaaaaat ttttcactac acgaattcct acgaatagta cagaacagac
    41101 agaagacctt tcagcagata cagcgaagaa aaagctgcag gaattagtcg atcaagaaga
    41161 caaaaacaag ccgttgtcgg accagaaatt ggttgaattg ctgaaaaaag atgagatagc
    41221 gatctcaaga cgaacagtag caaaatatcg tgatttatta ggaatcccga gttcatcaaa
    41281 aagaaaacgt tatgataact aatcagtcaa aagactagaa aaagtatttc actttttcta
    41341 gtctttttag ttgaaaaacg agagcgaaac tgttagactt acagtcgtga ggttgagatt
    41401 tccctttatt gatgtgaaat tcgaatagtt aatttctttt tttatcgtat atgggtcgtt
    41461 ttaagtctac attggacgta aagtgtccaa ctaaggagat cgctatgctg gatgaaataa
    41521 aaatgattga atctgttgca ccagatatta tcgaagtgtt gcaagaacgc tataaaattt
    41581 tacgaaatat ctattggatg cagccgattg ggcgtcgaag tttatcggaa agtatgggga
    41641 tcacagaacg agtcttacga acagaaacag accttttgaa aaatctgaat ttgatcgaca
    41701 cctcaaaaag cggtatgacg ctgacaacta agggcgaaga agtttatcaa agccttgaaa
    41761 acttcatgga tcagctttta ggtacgcatc aaaccgaaca acagttggca gaatattttg
    41821 gtaaccaacg ctgtatcgtt gtttcaggaa acagcgaaga gcagaccaaa gtggctgatg
    41881 cattcggcga agcgctgagc gaagccttag accgactact tccagaaggc gagaatatca
    41941 tcgctgtgat gggaggaact actatggctg tggtagccga acaattgtct aatctggaaa
    42001 ataagaaacg gcataacctg tttgtccctg caagaggcgg gattggtgaa gcgataactg
    42061 tacaggcaaa ttctgtcagt gcaagaatgg ctgctaaagc aaacggtaac caccgcgcac
    42121 tttatgttcc ggaacaatta agtctggcaa cttacaattc tttactcaat gagccatcta
    42181 tccaagaagt cttaaacttg attagtgaag cgaattgtgt tattcatagt attgggcgtg
    42241 cattacacat ggcagcacgt cgtaagatga ctgaaaaaga attagtcatg ctgaaacaag
    42301 caaatgctgt tgcggaatct tttggctact ttttcaatga aaaaggtgaa gtcgtttata
    42361 aggtccctag aatcggcata gaattgcaag atttggaaaa agtcccgatc atcatggcaa
    42421 tcgcgggcgg aaaatcaaaa gctaaagcaa ttcgagctta tatgaaaaat gcaccaaaac
    42481 aaacgtggct catcactgat gaagctgctg caaatgagat tttaaaaggg gtaacccttt
    42541 aaaataaata tttttttgat tttcataagg aggaaatctt aaatgacagt taaagtaggt
    42601 attaatggtt ttggacgtat tggacgtcta gcattccgtc gtatccaaga tgtagatgga
    42661 atcgaagtag tagcaatcaa cgacttgaca gatgctaaaa tgttggcaca cttgttaaaa
    42721 tacgacacaa ctcaaggacg tttcaacgga acagttgaag ttcatgaagg ttcattcaac
    42781 gtaaacggaa aagaagttaa agtattagct aaccgcaacc cagaagaatt accatggggc
    42841 gaactaggcg tagatatcgt tctagaatgt actggtttct tcacttctaa atcagcagct
    42901 gaaaaacatt taacagctgg tgctaaacgc gttgttatct ctgctcctgg cggaaacgat
    42961 gttccaacaa tcgtttataa cacaaaccac gaaacattaa ctggtaaaga aacagttatc
    43021 tcaggtgctt catgtactac taactgttta gctcctatgg ctaaaacatt gaacgacaaa
    43081 tttggtgttg ttgaaggatt aatgacaact atccacgctt acacaggtga ccaaatgact
    43141 ctagacggac ctcatcctaa aggtgacttc cgccgtgcac gcgctgctgc tgcaaacatc
    43201 gttcctaact caacaggtgc tgctaaagct atcggtttag taattcccga attgaacggt
    43261 aaattagacg gagctgctca acgtgttcct gtaccaacag gttcattaac agaattagta
    43321 acagttcttg aaaaagaagt aactgttgac gaaatcaatg cagcaatgaa agaagcttca
    43381 aacgaatctt acggatacaa cacagacgaa atcgtttctt cagatatcgt tggtatgact
    43441 tacggttcat tatttgatgc tacacaaact aaagtaatga cagttggcga caaacaatta
    43501 gttaaaactg ttgcttggta cgacaacgaa atgtcataca ctgcacaatt agtacgtact
    43561 ttagaatact tcgctaactt ataagataga caaacgtaca agtgtaaatt atagacatgt
    43621 acatttaaaa gcggggaagc aatcgcgctt cttccgcttt ttttgataat aagtttattc
    43681 aggaggaaat aaaatggcta agaaaacagt gaaagatatc gatttaaaag acaaaaaagt
    43741 tcttgtccgt gttgacttca acgttccttt gaaagacggc gtgatcactg acgacactcg
    43801 tatcaaagca gcattgccaa caatcaacta cgttctagaa caaggcggaa aagcaatcct
    43861 tttctctcac cttggacgtg taaaaacaga agaagataaa gaaggaaaat ctttagctcc
    43921 agttgcaaaa cgtctaggcg aattattagg taaagaagta acattcgtac ctgaaactcg
    43981 cggagaacaa ttagaagaag caatccgcaa catgaatgac ggcgacgttg tcgtattcga
    44041 aaatacacga tttgaagata ttgacggtaa aaaagaaagc aaaaatgata cagaactagg
    44101 taaatactgg gcttcattag gcgatgtatt tgttaacgat gctttcggta cagctcaccg
    44161 tgcacatgct tctaacgtag gtatcgcttc aactggtatc ccaacagttg ctggattctt
    44221 gatggaaaaa gaaatcaagt tcatcggtga agcagtagaa gaaccaaaac gaccaatgat
    44281 tgcaatcctt ggtggcgcaa aagtttctga taaaatcggc gtaatcgaaa acttgattcc
    44341 taaagcagat aaaatcttga tcggtggcgg aatgacttac acattctacg aagcaaaagg
    44401 aatcaagatc ggtaactctc tagtagaagc tgataaagtg gaactagcaa aagaattaat
    44461 cgaaaaagca ggcgacaaac ttgtcttgcc aatcgacaat gtctgtgcac cagaattttc
    44521 aaatgatgtt gaaacacaag taatcgaagg agacattcct gatggtttga tggctttaga
    44581 catcggccca gcatcagtaa aattatttgc tgatacatta caaggtgcaa aaacagttgt
    44641 ttggaacgga cctatgggtg tatttgaaat gagtaacttt gctaaaggta caatcggtgt
    44701 ttgtgaagca atcgctaact tagaaggcgc aacaacaatc atcggtggcg gagactcagc
    44761 cgcagcagca gaacaattag gatttgctga caaattcaca cacatctcaa caggtggcgg
    44821 cgcaagcttg gaattgcttg aaggtaaaga attaccagga ctagcagcaa tcaacgataa
    44881 ataaacgacc aagtttgctc aattctgacg agtaatcgaa caaaaggtga agggagtttt
    44941 catgactccc tagccatttt tcaataaatt aaggagtgtg ttcctcaatg cgtaaaccta
    45001 ttatcgcagg taactggaaa atgaacaaaa ctgcatcaga agcaaaagaa tttgctgaag
    45061 cagtaaaaaa caaaatccct tcaaatgaca aagttgattc agtcatcgga tctcctgcat
    45121 tattcttaca agaattaatg acatcagctg aaggtacaga actaaaaatc tcagcacaaa
    45181 actgctactg ggaaaactca ggtgcgttca ctggtgaaac ttcaccagca gctcttgctg
    45241 atctaggtgt tcattatgtg atcatcggac attcagaacg tcgtgaatat ttccatgaaa
    45301 cagacgaaga catcaataaa aaagcgaaag ctatctttgc aaatggtatg acaccaatcc
    45361 tatgctgcgg cgaatcttta gaaacctatg aagcaggcaa aactgctgaa tggatcgaag
    45421 gacaaattac agctggttta gctgatcttt cagctgaaca ggtgtcaaac atggttattg
    45481 cttatgaacc aatctgggca atcggtactg gtaaatcagc tgacgcacaa atcgctgacg
    45541 aaatttgtgg agttgtacgt caaacagttg aaaaacttta tggaaaagaa gtatcagaag
    45601 cagttcgtat ccaatacggc ggttctgtaa aaccagaaaa tattgctgaa tacatggcaa
    45661 aagaaaatgt tgacggtgct ttagtcggtg gcgcaagctt gcaagcagat tcattcttgt
    45721 cattgttaga cgctgtaaaa taattgttga aaatttgcga aaaatgcaag aaatgtttgc
    45781 actaatgagc aaaattaact aaaatcgtat atgagggaaa tcccttagga aaaaacaaac
    45841 tcaaaggaga gacaaaacat gtcaattatt actgatgttt acgctcgcga gatcttagac
    45901 tcacgtggta acccaacaat cgaagtagaa gtatacacag aaagcggagc ttttggccgc
    45961 ggaatggttc cttcaggtgc ttcaactggt gaatacgaag cagttgaatt acgtgacgga
    46021 gacaaagctc gttatggcgg taaaggtgta actaaagctg tcgacaacgt aaacaacatc
    46081 attgctgaag caatcattgg ctacgatgtt cgtgaccaaa tggctatcga caaagctatg
    46141 atcgctttag acggaactcc taacaaaggt aaattaggtg cgaacgctat tttaggtgtt
    46201 tctatcgctg ttgcacgtgc agctgctgac tacctagaag tacctttata ccactaccta
    46261 ggcggattca acacaaaagt attgccaaca ccaatgatga acatcatcaa cggcggatca
    46321 catgctgata actcaatcga cttccaagaa ttcatgatca tgcctgttgg cgctccaaca
    46381 ttcaaagaag ctctacgtat gggtgctgaa gtattccacg cattagcttc aatcttgaaa
    46441 gctcgcggat tagcaacttc tgttggtgac gaaggtggat ttgctccaaa ccttggttca
    46501 aacgaagaag gttttgaagt aatcatcgaa gcaatcgaaa aagctggcta tgtacctggt
    46561 aaagacgttg ttcttgctat ggatgctgct tcttcagaat tctacgacaa agaaaaaggt
    46621 gtttacgtac tagctgactc aggcgaaggc gaaaaaacaa ctgacgaaat gatcaaattc
    46681 tacgaagaat tagtttctaa atatccaatc atctcaatcg aagatggatt agacgaaaac
    46741 gactgggacg gattcaagaa attaactgac gtattaggcg acaaagttca attagttggt
    46801 gacgacttgt tcgtaacaaa cactcaaaaa ttatctgaag gtatcgaaaa aggaattgct
    46861 aactcaatcc ttatcaaagt gaaccaaatc ggtacattaa cagaaacttt cgaagctatc
    46921 gaaatggcta aagaagctgg ctacacagca gttgtatctc accgttctgg tgaaacagaa
    46981 gattcaacaa tctctgatat cgctgttgca acaaacgctg gccaaatcaa aactggttct
    47041 ctatcacgta ctgaccgtat tgctaaatac aaccaattac tacgtatcga agaccaactt
    47101 ggtgaagttg cagaatacaa aggtttgaaa tctttctaca acttaaaaaa caaataattc
    47161 ttttctaatg aagatacatg atcgactatt gaaagatagt cgagtatgat cacttagtta
    47221 agtaatcgaa ctctctactt gctgaaatat cagcaggtag agagtttttt tgcaactagc
    47281 ttctgtatat gatgtttaca aagaatggta caagaaaaga tcatccttga aaaaggaaag
    47341 ctaacaataa ctagcatcat ataagagctt ttaaggatag aacagtagaa taggaatcta
    47401 tgggcaagtc ctctcgtata aataaaatag gagggaagta gcagcatatt tttcataaag
    47461 tggctctctt tcttcatttt tactttggtt gctcctattt gaaaattcga gcacactcaa
    47521 atacaataaa aagaaatggc aagggagagg attagatgaa aaaagaagaa aaaatcgcga
    47581 ttttacaaga gattatccga attaaatcag tgaatggaaa cgaaggtgaa gtagcagcct
    47641 atttgaataa gcttttggca aagcatgaca taacaggaga gattgtttct tacagagatg
    47701 gtagagataa tttgattgct cgttaccaaa agggccaatc aggaaaagtg ttaggcctat
    47761 ctggacatat ggatgtcgtg gcggctggtg atgaatcgtc ttggacctat gcaccctttg
    47821 cagcagaaat ccatgggaac cgtttgtacg gacgtggtgc tacagatatg aagagtggct
    47881 tggcagcaat ggtgattgcc atgatcgaac tgaaagagtc aggaaaacca tttaacggaa
    47941 cagtcaaatt attagccact gtcggagaag aagtgggtga actaggtggg gaacagctca
    48001 cgaaagcagg ctatgtagat gacttagatg ccttgatcat cggagagcca accaattaca
    48061 gtttgatgta tacgcacatg ggatcaatca actatacagt cacctcgcac ggaaaagaag
    48121 cgcatagctc tatgccagac caaggttata atgcaatcaa tcacctaaat gaattcatta
    48181 cgaaagcaaa tgctgaaatg aatcacctcg ctgaaacaat cgagaatcct gtcctaggaa
    48241 aaacaatcca taatgttaca ttgatcagtg gcgggaatca agtcaacagc atcccaagcc
    48301 atgcgcagct ccaagggaac attcgatcga ttccagaata cccaaatgat aaaatcattg
    48361 cattgctgca atcaatcgtc aatgaattga atcaagagac agactatcat ttggaattga
    48421 tgatcgatta caacaaaata cctgtcaaag ctgatccaga ttctcctttg attcattgca
    48481 ttcagcaaca attcagccag ccattacctt tagttggagc ggcagcaaca acggatgcag
    48541 ccgaatttac gaaagctaac cattccttcg actttgtcgt ctttgggcct ggtgtcgtta
    48601 ctttgcctca tcaagtagat gagtatgttg aaatcgataa ttatttggat atgatcgaaa
    48661 aatatcaagg aattatttta tcctacttag cgtaagattt ttttaataa
//
LOCUS       DAJSAW010000016        47952 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJSAW010000016 DAJSAW010000000
VERSION     DAJSAW010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.016"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(38..1189)
                     /locus_tag="ONT64_001020"
     CDS             complement(38..1189)
                     /locus_tag="ONT64_001020"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR2302452.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="ONT64_001021"
     CDS             complement(1202..1639)
                     /locus_tag="ONT64_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2302453.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="ONT64_001022"
     CDS             complement(1654..3420)
                     /locus_tag="ONT64_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR2302454.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="ONT64_001023"
     CDS             3684..4361
                     /locus_tag="ONT64_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2302455.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="ONT64_001024"
     CDS             4364..5491
                     /locus_tag="ONT64_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2302456.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="ONT64_001025"
     CDS             complement(5691..6131)
                     /locus_tag="ONT64_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2302457.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="ONT64_001026"
     CDS             complement(6142..8175)
                     /locus_tag="ONT64_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR2302458.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="ONT64_001027"
     CDS             complement(8177..9880)
                     /locus_tag="ONT64_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2302459.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ONT64_001028"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ONT64_001028"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCR2302460.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="ONT64_001029"
     CDS             complement(10963..11988)
                     /locus_tag="ONT64_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCR2302461.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="ONT64_001030"
     CDS             complement(12197..13066)
                     /locus_tag="ONT64_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HCR2302462.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="ONT64_001031"
     CDS             complement(13525..15417)
                     /locus_tag="ONT64_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2302463.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="ONT64_001032"
     CDS             complement(15414..17141)
                     /locus_tag="ONT64_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2302464.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="ONT64_001033"
     CDS             complement(17157..17807)
                     /locus_tag="ONT64_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR2302465.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="ONT64_001034"
     CDS             18011..18307
                     /locus_tag="ONT64_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR2302466.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="ONT64_001035"
     CDS             18479..19099
                     /locus_tag="ONT64_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR2302467.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="ONT64_001036"
     CDS             complement(19191..20357)
                     /locus_tag="ONT64_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR2302468.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="ONT64_001037"
     CDS             complement(20369..20722)
                     /locus_tag="ONT64_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR2302469.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="ONT64_001038"
     CDS             20850..21872
                     /locus_tag="ONT64_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCR2302470.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="ONT64_001039"
     CDS             21891..22052
                     /locus_tag="ONT64_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302471.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="ONT64_001040"
     CDS             complement(22311..22745)
                     /locus_tag="ONT64_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302472.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="ONT64_001041"
     CDS             complement(22855..24714)
                     /locus_tag="ONT64_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR2302473.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="ONT64_001042"
     CDS             complement(24832..25992)
                     /locus_tag="ONT64_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HCR2302474.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ONT64_001043"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ONT64_001043"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR2302475.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="ONT64_001044"
     CDS             complement(27739..28437)
                     /locus_tag="ONT64_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR2302476.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="ONT64_001045"
     CDS             complement(28394..30055)
                     /locus_tag="ONT64_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HCR2302477.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="ONT64_001046"
     CDS             30273..31673
                     /locus_tag="ONT64_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCR2302478.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="ONT64_001047"
     CDS             complement(31925..33004)
                     /locus_tag="ONT64_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2302479.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="ONT64_001048"
     CDS             complement(33090..34049)
                     /locus_tag="ONT64_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCR2302480.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="ONT64_001049"
     CDS             complement(34061..35572)
                     /locus_tag="ONT64_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCR2302481.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="ONT64_001050"
     CDS             complement(35594..36157)
                     /locus_tag="ONT64_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCR2302482.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="ONT64_001051"
     CDS             complement(36163..37026)
                     /locus_tag="ONT64_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2302483.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="ONT64_001052"
     CDS             complement(37040..37936)
                     /locus_tag="ONT64_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2302484.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="ONT64_001053"
     CDS             complement(38111..39400)
                     /locus_tag="ONT64_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2302485.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="ONT64_001054"
     CDS             complement(39372..40889)
                     /locus_tag="ONT64_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCR2302486.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="ONT64_001055"
     CDS             41087..41977
                     /locus_tag="ONT64_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR2302487.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ONT64_001056"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ONT64_001056"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR2302488.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="ONT64_001057"
     CDS             complement(42886..44358)
                     /locus_tag="ONT64_001057"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR2302489.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44995..45357)
                     /locus_tag="ONT64_001058"
     CDS             complement(44995..45357)
                     /locus_tag="ONT64_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCR2302490.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45580..46332
                     /locus_tag="ONT64_001059"
     CDS             45580..46332
                     /locus_tag="ONT64_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR2302491.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46472..47395)
                     /locus_tag="ONT64_001060"
     CDS             complement(46472..47395)
                     /locus_tag="ONT64_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2302492.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47410..47682)
                     /locus_tag="ONT64_001061"
     CDS             complement(47410..47682)
                     /locus_tag="ONT64_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302493.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        13979 a         9954 c         8268 g        15751 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgtttttt
    44941 attgtataca ttagatatgg agcaggttac cataaagatg attattagtc gttattaaat
    45001 agaatccttc gtattttttt gttctatcac tacaatatct ttatttggac gtttgaattg
    45061 tattccggca cgttctagct cctgtttcac tcctacatgg actttgacat cttctcgtgt
    45121 catttttaaa acgtgatgaa tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt
    45181 attgatcatc acgacttgct ttttatcata tgtgtctacc aatagcagtt gtgttcgatc
    45241 taacgaaaat aatttgtaat aattaatagc cgtattccca aaaaagaatt ccactacttt
    45301 aggataaaat gtcttaatat ttaaattact attcacaggt gtttcgatta gcttcatctc
    45361 attcctcttt tctatatagt cgatttagat ccaatcttaa gttatgttac catctttcct
    45421 agcattaagg aatcggttac aattgaacct ttaaagacaa aaggatttac cttttatcac
    45481 ttaatagtat aacataattt catgagaaaa aaatacaatt ttttcttgac catctcgtta
    45541 cgtaaaggat tatgcttaca tttgtcagga ggaaaaataa tggagtacac aataaagaaa
    45601 atgtcagaga tatcaggagt cagtccgaga accttgcgct tttatgatga gattggactg
    45661 ctaaaaccag ctaggatcaa ttcttcaggt tatcggattt atggcaaaaa agaagtcgat
    45721 cgtctgcaac acatcttatt ttatcgaaca atggcattca agttggatga tatccaagag
    45781 gtcttagaca atccatcttt tgaccaccag aaagctttaa tcaagcatca gcaaatgttg
    45841 ctggaaaaac gggcacaaat cgataccctc ttgacaactg tccaacagac acttgatatg
    45901 tacgaaggag gaagaaaaat gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag
    45961 ttgaaagaaa atgaagaatc ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag
    46021 gttacagcat ccaatgaaaa atggatgaat atggataaag aaacgtatga agaaatgcaa
    46081 actgttgaaa agcagatgtt atttgatctc actctttact taaaagaacc agaagatgaa
    46141 agcttagcag accgtatctt ccaagctcat aagaagtggt tatcttacag ctggcctgaa
    46201 tatcagccaa aagcgcataa aggactagga atgatgtacg aagccgatga acggttcact
    46261 gcatattatg acgagcgaag tggtcatggt gcagcaaaag ctttaaatga aattatccaa
    46321 aaaaaggcgt aatcttttac atgatccaac aaaaaagtac acgtaaaatg acctaataac
    46381 aaaacagcag acacccaagt tagattttta tgctaacttt tggtgtctgc tccattcttt
    46441 cacaagtttt ttcatcctac tttaaatata ttcatcgttg aataaacgta taaggtattt
    46501 tcactttatc ctttttttct gtaagaaaca attgaaatgc catttctccg caagcttcca
    46561 gatggtggtc gatggttggg aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa
    46621 tcaccactgg tgtcttatct tgcagatagc tttgcaagat gcctgctgca accatatccg
    46681 aatttgtaaa gacaccatca actccaagct ctttaaaaaa gaagcctgct tgttttcctt
    46741 tctctgcatc tatacaatcc caaaaaacaa gtgattcatc aaaagatgga aaaagttgat
    46801 gacatagctg caaagtgatt tttgagttat aacttagcct gccacttcgc cccaacgtga
    46861 tgcccaattg ctttatacct tgctgcttca aataagtaag accttctgta attgattgtt
    46921 ctcgatcaat aaagacacag cttgcctgtt ttcccttgat ttcttcacaa aaaactagag
    46981 gtccatattt ttgatatgcc aataaatcat ctagtgtatt ggcacgagac gtaatgatca
    47041 atccatcgaa agctttagct gcaaattctt caagatacct tcgctctttt tctttctgat
    47101 agcccgtcgg taaaagtgtc actttgtaat cataatcaaa cgctgcttca atgatcccac
    47161 tgatcaactg atcaaaatac ggatgattgg tataaggcag aatcactcca atgcttttcg
    47221 tttttccata acttaactgt ctggcatttg cattagggat ataatcgact tctttcatga
    47281 ccgcaagtat ttctttcctt ttttcctcat ctacatatgg atgtccattg attacacgtg
    47341 aaatagtaga aactgaataa cctgtgagct ttgcgatatc tcgaatgttt gccactgctg
    47401 cttcgccacc tatacaaaaa gattttcttg taaatagtct accacagctg gaccgcattc
    47461 aaaaaactga tacatttttc cttcatgcga aaaactgatc cgctgcccgc cccaatatat
    47521 atcttttgtc aaattttcga tttcattcat tggtatttct tttactatat tcctattcaa
    47581 cccttgaaaa ctgtacaacg ttaatacgga tcccttgaac tcgacaaacg cataaaaata
    47641 attgttcgtt gtgccatctg ttatctctac ataagtattc attgcaagcc ctccttttga
    47701 tactttgagt atagtgtatg aaacgcgtct catagcaagg agtttgtttt gccaaagaaa
    47761 agttttattc ctctttcatt attaccaaag tctcttattt taaattgaat acattataat
    47821 gatactgtaa atatatcttt attattagtg aatggatatt tacttttaat agataaaata
    47881 ttataactat taaaaataac tattttcatt ataattacac acaaaaaaga gcgtgagaca
    47941 aaagtaaaga ac
//
LOCUS       DAJSAW010000017        47762 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJSAW010000017 DAJSAW010000000
VERSION     DAJSAW010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.017"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            83..2248
                     /gene="acm"
                     /locus_tag="ONT64_001062"
     CDS             83..2248
                     /gene="acm"
                     /locus_tag="ONT64_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HCR2302494.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2520..3005)
                     /locus_tag="ONT64_001063"
     CDS             complement(2520..3005)
                     /locus_tag="ONT64_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HCR2302495.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3095..3440
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3485..4126
                     /locus_tag="ONT64_001064"
     CDS             3485..4126
                     /locus_tag="ONT64_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR2302496.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4145..4780
                     /locus_tag="ONT64_001065"
     CDS             4145..4780
                     /locus_tag="ONT64_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR2302497.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4798..5613
                     /locus_tag="ONT64_001066"
     CDS             4798..5613
                     /locus_tag="ONT64_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2302498.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5932..7368
                     /locus_tag="ONT64_001067"
     CDS             5932..7368
                     /locus_tag="ONT64_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2302499.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7557..10004
                     /locus_tag="ONT64_001068"
     CDS             7557..10004
                     /locus_tag="ONT64_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR2302500.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10234..11301
                     /gene="rlmN"
                     /locus_tag="ONT64_001069"
     CDS             10234..11301
                     /gene="rlmN"
                     /locus_tag="ONT64_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCR2302501.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11494..12597)
                     /locus_tag="ONT64_001070"
     CDS             complement(11494..12597)
                     /locus_tag="ONT64_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR2302502.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13150..13698
                     /gene="aac(6')"
                     /locus_tag="ONT64_001071"
     CDS             13150..13698
                     /gene="aac(6')"
                     /locus_tag="ONT64_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCR2302503.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            14244..15905
                     /locus_tag="ONT64_001072"
     CDS             14244..15905
                     /locus_tag="ONT64_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2302504.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(15975..16322)
                     /locus_tag="ONT64_001073"
     CDS             complement(15975..16322)
                     /locus_tag="ONT64_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCR2302505.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            16406..17347
                     /locus_tag="ONT64_001074"
     CDS             16406..17347
                     /locus_tag="ONT64_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2302506.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            17348..18391
                     /locus_tag="ONT64_001075"
     CDS             17348..18391
                     /locus_tag="ONT64_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2302507.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            18397..19467
                     /locus_tag="ONT64_001076"
     CDS             18397..19467
                     /locus_tag="ONT64_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2302508.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            19457..20410
                     /locus_tag="ONT64_001077"
     CDS             19457..20410
                     /locus_tag="ONT64_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2302509.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            20422..21432
                     /locus_tag="ONT64_001078"
     CDS             20422..21432
                     /locus_tag="ONT64_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2302510.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(21717..22538)
                     /locus_tag="ONT64_001079"
     CDS             complement(21717..22538)
                     /locus_tag="ONT64_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR2302511.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(22635..22913)
                     /locus_tag="ONT64_001080"
     CDS             complement(22635..22913)
                     /locus_tag="ONT64_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302512.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            23181..25190
                     /gene="metG"
                     /locus_tag="ONT64_001081"
     CDS             23181..25190
                     /gene="metG"
                     /locus_tag="ONT64_001081"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR2302513.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(25669..26256)
                     /locus_tag="ONT64_001082"
     CDS             complement(25669..26256)
                     /locus_tag="ONT64_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302514.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            26431..27753
                     /locus_tag="ONT64_001083"
     CDS             26431..27753
                     /locus_tag="ONT64_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2302515.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            27908..28684
                     /locus_tag="ONT64_001084"
     CDS             27908..28684
                     /locus_tag="ONT64_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCR2302516.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            28677..29246
                     /gene="rnmV"
                     /locus_tag="ONT64_001085"
     CDS             28677..29246
                     /gene="rnmV"
                     /locus_tag="ONT64_001085"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCR2302517.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            29239..30123
                     /gene="rsmA"
                     /locus_tag="ONT64_001086"
     CDS             29239..30123
                     /gene="rsmA"
                     /locus_tag="ONT64_001086"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR2302518.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            30574..31203
                     /locus_tag="ONT64_001087"
     CDS             30574..31203
                     /locus_tag="ONT64_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302519.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(31327..32436)
                     /gene="ugpC"
                     /locus_tag="ONT64_001088"
     CDS             complement(31327..32436)
                     /gene="ugpC"
                     /locus_tag="ONT64_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCR2302520.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            32645..33067
                     /gene="mgsA"
                     /locus_tag="ONT64_001089"
     CDS             32645..33067
                     /gene="mgsA"
                     /locus_tag="ONT64_001089"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR2302521.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            33390..33926
                     /locus_tag="ONT64_001090"
     CDS             33390..33926
                     /locus_tag="ONT64_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCR2302522.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(33966..35729)
                     /locus_tag="ONT64_001091"
     CDS             complement(33966..35729)
                     /locus_tag="ONT64_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2302523.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(35732..37447)
                     /locus_tag="ONT64_001092"
     CDS             complement(35732..37447)
                     /locus_tag="ONT64_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2302524.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(38226..38450)
                     /locus_tag="ONT64_001093"
     CDS             complement(38226..38450)
                     /locus_tag="ONT64_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302525.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            38641..41418
                     /locus_tag="ONT64_001094"
     CDS             38641..41418
                     /locus_tag="ONT64_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2302526.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            41499..41918
                     /locus_tag="ONT64_001095"
     CDS             41499..41918
                     /locus_tag="ONT64_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HCR2302527.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            41930..42400
                     /locus_tag="ONT64_001096"
     CDS             41930..42400
                     /locus_tag="ONT64_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2302528.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            42417..43196
                     /locus_tag="ONT64_001097"
     CDS             42417..43196
                     /locus_tag="ONT64_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2302529.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            43183..44004
                     /locus_tag="ONT64_001098"
     CDS             43183..44004
                     /locus_tag="ONT64_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2302530.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            44021..45070
                     /locus_tag="ONT64_001099"
     CDS             44021..45070
                     /locus_tag="ONT64_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR2302531.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            45083..46087
                     /locus_tag="ONT64_001100"
     CDS             45083..46087
                     /locus_tag="ONT64_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR2302532.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            46720..47739
                     /locus_tag="ONT64_001101"
     CDS             46720..47739
                     /locus_tag="ONT64_001101"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR2302533.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        15812 a         8305 c         9867 g        13778 t
ORIGIN      
        1 gtgggggagg aattgaagaa aggatttttg agagatgata tagtaggttt tatttttagt
       61 taaaataggg gagatagaaa aaatgaaaaa gtgtagaaat attattttat caatgttatt
      121 tattattact aatataacat cactgattcc tgttcatgtc tatgcggatg caggcagaga
      181 tatcagcagt aatgtcactt cgttgaccgt ggacccaacg aatattacag atggaggaaa
      241 cataaaagta aaattctcgt ttgacgaaaa aaaacaaaat atccaaccag gtgattatct
      301 ttggatcaat tggccaagtg aaggaaatat tcgcggtgaa ggattccaaa aagaaatccc
      361 attaatgatt gaaaataaaa atgttggaac tttaacggtc agaaaagatt ctgcgcaggt
      421 tgtgtttaat gaaaatatta aaaacctaga ctcagtagaa ggttgggggc aatttgagat
      481 acaggccaga aacgtaaccg ataccggtga agaaaatacg ggaccattca ccgtgacgag
      541 cggtgataaa acagctactg taaatgtgac aaagccagct tctggttctt cttctagcgt
      601 tttttattat aaaacaggag acatgctacc tgaagatact aagcacattc gatggttttt
      661 gaatattaac aataacggca cttatgttga acaaccagtt aaaatcagtg atgaaatcca
      721 aagtggacaa agattagatc caagtacgtt tgaaattaat caaatacact taggtgaaca
      781 gaaggtttat agaggagaag agggaatcca acaattttta caagatttcc ccagcgctac
      841 atttaatttt tcagtaactg ataattacat tgaaataact ataccaaaaa actttgtcaa
      901 tcttaggaaa ataatggtga gttataagac gatcatagaa aacccagaac aaataaattt
      961 tgagaatcat tcggaagctt ggtttaaaga atttaataaa cctgctgttg atggagaaag
     1021 tttcaatcat acagttaaaa atatttcagc ttctggtgga gtcaacggta cagttagagg
     1081 cgaattgaag attttcaaat atatcaatga cacagagatt ggtatcccaa atgtgacttt
     1141 cgaattaaga cgagcagatg aacagcctat tcaaggacag tctagtatcc tgttgacttc
     1201 gaatgaacag ggagaagcta gtatcaaagg cctgcaagta ggagactatg ttgtaaaaga
     1261 aaaagaagca cccaattgga ttgattttga tccactaagt tcaaatgaac tcaaattctc
     1321 cataaacgaa aatgatacag agggagtcag tttacctatt tataacaaga aaaaggttac
     1381 gaacatcaca gcaactaaaa tttggaacgg tggaaccact ccaagaccga gtatttattt
     1441 taaattattc agagcaagta caaacaattg ggaaccagtt cctgatgcag aaacaaaacg
     1501 attagacaat ggaataactt cagtgacttg ggaagatatc caacagtatg atgatagtgg
     1561 aaacgaatat acgtttaaag tgcaagaagt agatcaaaac ggaaatgatt atgtcccttc
     1621 aggttatcaa aaaattgaaa acgggctagt cgttacaaat gagaataaag aagtcatttc
     1681 agtctcagga caaaaaacat gggaagacaa tgagaaccaa gacggcaaac gaccgacgac
     1741 gatcacagta aatctattag ccgacggtca accgatccaa cacaaagaag taagtgaaaa
     1801 agatgattgg cgctatcgtt tcacaaatct gccaaaatac agagacgggc aagaaatcat
     1861 ctatactgta acggaagaca acgtgcccga atacagtacg acgattgacg ggtataacat
     1921 caaaaacagt tatacaccgg aaaaaacaaa tatatctata ccaggtagcc atataacacc
     1981 gtggaaaaat tttccagaga agacagagag agagaaaatt agaaatctat tctctcaaaa
     2041 attgcaaata tttgctcata caggagaatc cagcgaacaa agaagcaatt atattagtaa
     2101 gagagctgta cctaaacaga taacaaataa atcaaaaagc attcttccta aaacaagtag
     2161 taaaaaatct agttttgctg taatcattgg tttattgata gtaactatct gtgctgggat
     2221 attcttacaa aaatataaaa aagtttagtt agaatataat attttttgtt aaatatgact
     2281 gaaatcaaaa gaataaaatt ccgatttata atcgaaaaaa gaagcatttt tatatatgct
     2341 tcttttttcg tagaatcaat aaatatggat ttggctaact atggaagaat actcgtatta
     2401 gttttcagcg ttcatactag ttgttacatt gtttgattgg ccaaaagaat ttctgatata
     2461 ttttgtgcta aataaagaaa aagacgcttg aattgggaaa taagaaaact actctatcat
     2521 tacaaatttt cataaactgg cagatgttct aagtaaacaa gtatcttttc aaagtcggaa
     2581 aaatccataa tcaacgtagt ggtattgata tttggatgaa agcctacggt atcttgacga
     2641 tcaatagcag catcgatcac gacttggatt tttttctctt tgtcgtgcat caatgctaat
     2701 ggagtcactg tacctgctgg aacgcctagg aggtcaggaa gctgttcttc agaaacaaaa
     2761 gataagcgct tttcttttaa ttgttcagct agttgtttta aatcggcgtg cttttcatca
     2821 ggcaagatga caagatagat ttgttttcct tttcttgatt tcaataatag attttttact
     2881 tgcggaccag gtagggaaaa agggacattt ttgactgacg taatcgcagg atgatcgact
     2941 ttttgaaaag agatacccag tttttttagt agttcataag cttcttgttc ggttgcacga
     3001 ctcatcgcgc atcaccctcc tttttttatt agtgtatcat aaaaaagagt tgacaaatga
     3061 aaaataatca tgtaaattac acgtaaagtt aaaacaaatg acatcgaaaa gaagagtagc
     3121 cgttaggtgt ttttagagag tctctggttg gtggaaagag gcagcagtta accgtgaaac
     3181 acatcttgga gtcgattttc tgaagcgtat agtaggaaaa tccggggagc ccgttacagc
     3241 tgcggtcgtt gctagatgac cagataaggc tggcttttcc gtgaggagtc ggtaaaaaaa
     3301 ggtggaacca cgtgattaac gtcctttgat cttcggatca aaggactttt tgttttcatc
     3361 tgaacggcaa cgactgtgtg aggttttttc tgcaaggaaa aaataaacag aggtggaacc
     3421 acgtataata cgtcctcttg gcatttgcca agaggatttt tttattttaa aggaggaaaa
     3481 gaagatggag tatttgattg aagttatgcc gcaattatta gtaggagcac tcacaacatt
     3541 gaaattattt ttatttacct tgattggctc attaccatta gggattgttt tagcattagg
     3601 attgtctttg cattttaagc cgctacgcta cttgcttcaa acatatgtat gggttatgcg
     3661 gggaacaccg ctgttgttgc aactgatttt tattttttat gggttgccga cgattgggat
     3721 cgtttttgat cgtttcgaag cagcactcat tgcctttatc ttaaactatg cagcttattt
     3781 tgcagagatc tttcgtggag ggatacaatc gattcctcaa ggtcaatatg aagcagcgca
     3841 agttttgcaa ttaagtccct ttcaaacggt gacgaaaatc gtcatcccac aagtgacaaa
     3901 gattgtttta ccttctgtag ggaatgaagt tatcaacttg gtcaaagatt cttccttgat
     3961 ctatatatta ggtttaggtg acgtaatgcg tgctgggaaa atcgcgatgg aacgcgacgc
     4021 gactttactg cctttgctag gcgtggcaat catctatctt acattgactg gtattctaac
     4081 aatcataatg aaacaagttg aaggaaaatt aaattattac cgctaactga taggaggaag
     4141 aaatatgatt gaattgaaga aagtgaacaa agcatttggt gaaaaaaaaa taatcaaaga
     4201 tttgaatcta atcattccag atgggaaaac attagctatc gtaggtcctt ccggtggcgg
     4261 taaaaccacg cttttacgta tcttggctgg cttagaaagt tctgacagcg gagaatttta
     4321 tttagacggt gtaccgttca acccattcga gcttaaaatg aaagaacaag ttgtaggagt
     4381 cgtattccaa gacttccagc tatttccaca tctgaatatt tttgaaaaca ttaccttagc
     4441 accaaaaatg gtattgaaac agacaaaaga agaatacacc aaaaaagcag aagaattagc
     4501 ggcttcatta gggattagtg atttattgaa tatctatcct tatcagcttt ctggcggaca
     4561 aaaacagcgg gtagcgattg ccagagcatt ggctatgaat ccacgggtat tggcttatga
     4621 tgaaccaaca agtgctttag atcctgaatt acgccagcaa gtcgagcgtt tgatcctatc
     4681 attgaaagaa gatggggtaa cccaaattat tgtcacccat gatttagtat ttgcagaaaa
     4741 tgtcggtgat caattactaa aagtttcccc aattcattaa gaaaggagaa gatagagatg
     4801 aaaaaaacca gttttctatt cgctttgatc gcaggtcttc ttctttttgc aggctgttcg
     4861 aacaaaaaaa cttcagcaga taattgggcg actatcaacg aagagaaaaa aatcgttata
     4921 ggtttagacg atacatttgt tcctatggga tttagagata aagacgggaa gctcacagga
     4981 tttgatattg atttagccaa agcagtcttt gaacaatatg ggattacggt agactttcag
     5041 cccattgact ggtcaatgaa ggaatttgaa ctgaacaatg gaacgatcga tttgatatgg
     5101 aacggctatt ccaaaacgcc ggcaagagaa aagaaagtgc aatttacaaa accttatatg
     5161 gaaaacgatc aagtgctgat cacacctaaa gaatcaggaa tcacaaactt tggacaaatg
     5221 aataataaga gattaggtgc gcaaaatggg tcatctggtt atgatgtatt tacgaaacaa
     5281 ccagaagtat taaaagagat agtcaaaaac caagatgctg ttttgtatga tagttttaat
     5341 gaagcattga tcgatctgaa aagcagacgg gtcgatggtc tgctgatgga taaagtatac
     5401 gcagactatt atttgaaaca aagaaacgaa ctagaagact tcaacataac aaaaggttct
     5461 tatgagagtg aggattttgc agttggtgca cgcaagtcag atagtacatt agtgaaaaaa
     5521 atcaatgaag ccctagatca actagagaaa acaggtgaat tccaaaaaat atccgataaa
     5581 tggtttggcg aagatgttac gccgagaaat taacaaatag gggactgtga caaaagacgt
     5641 gtcacagtcc ctttttccga ataaacagtg ttctcctgcc aactccttgt gactcggagc
     5701 tgaatggcag gctcacaacc tcttggtaaa cggtgttcaa aagccagttt ttcggtatta
     5761 agccaaattt atgcaaaaat gtagaaagcc attttcacaa aatttttctt actactcgaa
     5821 actaagcgac tttttcacaa cctcctttac ataattttat tttgttgtgg aaggggtttc
     5881 gtgttattct aaaagaggaa cgattgaagg ttataggaaa ggaagagatg aatggaacac
     5941 aaagaactta cctcatttcc aaaagatttt ctgtggggct ctgcttctgc agcgtatcaa
     6001 gtagaaggag cttggcaaga agatgggaaa ggagaatcag tctgggatcg atttgtacgg
     6061 atacctggaa agacatttaa aggaacgaac ggtgatttgg cagtagacca ctatcatcgt
     6121 tataaagaag atattgcttt aatgaaagaa caaggtctga aagcttaccg cttctctgta
     6181 gcttggacac ggatttttcc taatggacga ggagaagtca atcaagctgg gctcgctttt
     6241 tatgagagat tgatcgacga attgattgaa aacgatattg aacctatgtt gactttatat
     6301 cattgggatc ttccacaagc attgcaagat gaatataatg gttgggaatc acgtcagatc
     6361 attgaagatt tcactcatta tgcagaaaca ttatttgagg cattccgcgg gaaagtccat
     6421 tactggatta gtttgaatga acaaaatatc ttcacttcat taggttattt gttagcagca
     6481 catccgccag gtgtgacaga cccgaaacga atgtatgaag ttaaccatat tgctaattta
     6541 gcaaatgcat ctgtcatcaa taaatttcac gagttagaaa tgcctggaaa aatcggacca
     6601 agctttgcgt atacgccaaa ttatccaatt gacagtaatc ctgaaaatgt tctggctgct
     6661 gaaaatgcag aagacctgat ggccaattat tggatggatg tctatatgtg gggaaaatat
     6721 ccgattgctg ccatgaggtt cttagaagaa aaaggatggg caccaacgat tgaagctggc
     6781 gatgcagaat tgctagaatc tgctaaacca gattttcttg ggatcaatta ctatcaaacg
     6841 gcgacgaatg cattcaatcc attaaacgga gttggtgctg gaaaaatgaa tacgacagga
     6901 aaaaaaggtt catcagaaga aactggagtt cctggaatgt ataaaaaagt tgagaatcca
     6961 tttgtcgaac gaacaaattg ggactgggag attgacccag aaggattaag gatcggactg
     7021 cgtcgtatca caagccgtta ccgcatgccg gtactgatta cggaaaatgg cttaggagag
     7081 tatgacaagt tgacagaaga taaacaaatc catgacgact atcgaatcaa ttacttacaa
     7141 agccacgtca aagccatcaa agaagcaatt tcggatggag cagaagtcct aggctattgc
     7201 acctggtctt atacagattt gcttagctgg ctgaacggtt atcaaaagcg atatggattt
     7261 gtatatgttg atcaagacga aacacaagaa ggatctttgg aacgatataa gaaagacagt
     7321 tattactggt accaaaaact gattgaagaa aatggagaga acctttagtt cacatactta
     7381 aaacaagaag gtacggctgg atatggccgt gccttttctt gataaaatca tttagaaatt
     7441 aagaaaaact gtaaaataag ctgaaaaatg aaagaaagcg tcagttcttt caaacaaaac
     7501 tttagttccc ttactttttt ttgtataatg aaacttgatg aaatgaggga taataaatgg
     7561 cacaaaagaa acggcctact aaaaaaagga aaaagacgaa aaaaacgaaa aagcaaaaac
     7621 agcagcagga acaaatgctg acgattggtg ttgggtttgc cttgatttta tttgctgtct
     7681 tcggcttttt aaaattaggt tttttaggaa tattgattgc aaacggcttt cggatcattg
     7741 caggaaatac ttaccaaatc ctctgtcttt tattagccgt attaggattt tggattgtca
     7801 ttaagaatac agagttttca attggtaaaa accgacgctg gtttggcggc atactctttt
     7861 atttcggtat cttattattg ttacatgccc atttatttgg caaactccat actggggaac
     7921 caaatatcat ggggacgaca tgggatttgt tagccagtga tataaaacaa agtcaagtag
     7981 acaataacgt tggtggtggg atgattggag caatccttta tcattttact tacttcttga
     8041 ttgcacagcc tggaagttat ttagcagcag tgctattact ggcaggagga gcattcctgt
     8101 tcagtaatct ggaaggctat cagctgttaa atggtatcca atcaattgga gaacgagtac
     8161 aggaattatt ggaaggtgat cctgccaagc aagctcgaaa gcaagcggca aaggaagaga
     8221 gaatgaaaca gcgcgcagaa gcgaaagaag caaggcgctt agctgcaaaa gaagctgcag
     8281 aaaaagaagc tgcagaatat gagaagaaaa aagcggtgag ccagcaaagg gaaaatactg
     8341 ctgatgaatg gcaggaacca gaaaatacag agccggagca gctaagtttt gttccaatcg
     8401 acagttttca agaaaatatc catcctgcga atctagagaa accagtacca gatacaccaa
     8461 aacagaccaa tacagcagaa ggtttttcag atgagttgcc agaggatgac ggtacctcat
     8521 tagaatttga gattgaagca gaacaggaaa atcaagacta tgaattaccg acagttgatt
     8581 tgcttgattc tataccaact gtggatcaaa gtgatgagta taaaaaaatt gaaaaaaata
     8641 tcggcgtatt agaacaaact ttccaaagtt tcggtgtcga tgccaaggtc gtcaaagcca
     8701 gcttaggacc atccgtcaca aaattcgaag tccagccggc tgtaggggta aaagtaagta
     8761 aaattgtgaa cttaacagat gatatcgcgc ttgctttagc ggcgaaagat gtccggatgg
     8821 aagcaccgat tccaggtaaa tcactgattg gtattgaagt accaaatgga aagatcagta
     8881 tggtttcctt tagagaaatc atcgaagcac aaccaaacca cccagataaa ttactagaag
     8941 tacctttagg acgtgatgtt tctggacggg tacaaacggc tgatctttcg aagatgcctc
     9001 acttactcgt ggctggttcg actggtagtg ggaagtcagt agcaattaat gggattatca
     9061 caagtatatt aatgcgtgct aagccccatg aagtaaaact gatgatgatc gatccgaaaa
     9121 tggttgaatt gaatatgtac aacggcatac ctcatctgtt gacgccggtc gtgacgaacc
     9181 ctagaaaagc agcgcaagcc ttacaaaaag tagtacaaga aatggaagaa cgatatgaaa
     9241 aattcgcggc aaccggagta agaaatattt ctggttacaa tgaatttgtt caacagaaaa
     9301 acttggaaaa cggaacgaaa catccaacct taccattcat cgtagtgatc gtagatgaat
     9361 tagctgattt gatgatggta gccagcaatg aagttgaaga tgcgattatt cgtttagcgc
     9421 aaatggctcg ggcagcgggg attcatatga tcctagctac tcaacgtcca agtgtggatg
     9481 ttatcacagg aatcatcaaa gcaaatgtac cttccagaat ggcatttgcg gtatcaagcg
     9541 gtacagattc tcggacgatc attgattcta atggggcaga aaagctctta gggcgaggag
     9601 atatgctatt cttgccaatg ggtgagaata aaccgatccg tgtgcaagga gcattcattt
     9661 ctgaccacga agtagaacgg gtcgttcaat ttgttacaga tcagcaagaa gctcattatg
     9721 aagagaaaat gatgccgaca gatgaagttg aaacgacagg tgctccagaa cagccacaag
     9781 atgagctgtt tgaagaagca aaagcacttg tcgttgaaat gcaaacggca agtatttccc
     9841 ttctccaaag aagatttcgg atcggttaca accgagcagc acgtttagta gatgaactgg
     9901 aagctcatgg agttgttggc ccgtcagaag gaagcaagcc tcggaaagta ttcattgagc
     9961 aggaagaaac atcagaaacg ttgccggacg ataatgacat gtaaaacttg tgtgatgaag
    10021 ggttgaaaaa cgaataacca ataaaaagac aaggcatgat gataccaaaa gaaaaaatgg
    10081 tttttttcca tcttttctaa tacggtatga tgtcttgtct ttttagtttt tgtgagatta
    10141 aagttctatt cgatttattt gtgaagtatt gcattaagga aatgaatcat gtatagtatg
    10201 tagcgaaact ttgagaaaaa gagggaaaat actttggaaa aacagtccat ttatggcttg
    10261 acgaatgaag aattaatcaa ttggtttata gaaaacggcg agaaaaaatt ccgagctgcc
    10321 caagtttggg aatggttgta tcaaaaacgt gtaagtaatt tcacagaaat gacgaatcta
    10381 tcgaaacaat tgatcgaaaa attatcggag cattttatca tcaatcctct aaaacagatg
    10441 gtcgtccaag aagcttcgga tggtacagta aaatatttgt ttgaactccc tgataaaaac
    10501 atgattgaga ctgtactgat gagacaagaa tatggcttgt cagtttgtgt gacgacacaa
    10561 gtaggatgta atatcggctg tacgttttgt gcaagcgggc tgctcaaaaa gaaccgtgat
    10621 ctgactgctg gagaaattgt tgcacagatc atgatggtcc aacattattt tgatgagcgc
    10681 aatcttggcg aacgcgtttc acatgtcgtt gtaatgggga tcggtgagcc atttgataat
    10741 tacgacaatg taatggactt tttgcatatc atcaatgatg ccaaaggatt ggcaattggt
    10801 gctcggcaca ttacagtttc cactagtgga ttagcacaca aaatcaagga attcgctaat
    10861 aacggcttgc aagtcaatct ggctatttcc ttgcacgcgc cgaataatga ggtgcgaaca
    10921 tcgatcatgc ggatcaaccg aagtttccca attgagaaat tgatggaagc tgtggatgag
    10981 tatttagaga aaacaaaccg tcgaatcacg tttgaatata tcatgttgaa tcaagtaaat
    11041 gatagaccag agcatgcgca acaattagca gatctattaa aagataagaa aaaattagca
    11101 tatgtcaact tgattccata taatccagtc agtgaacatg atcaatattc tcgcagtcca
    11161 aaagctgatg tattgagatt ttatgatgta ttgaagaaaa acggcgtaaa ctgtgtgata
    11221 cgtaaagaac atggtacaga tattgatgca gcttgcggac aattgcgaag caagcaaatg
    11281 aaaaaaacag ttgccaaata atcgtatcct actcgttttt tcaattacca taaaaggagc
    11341 agttttctta attaaggaac tgttcaactt aaataagaga aaagattcta tagcgaaagc
    11401 tataaagctt tttttgacaa gaaaaaccct catcaagcaa agaaatgtcc tcaatttgat
    11461 gacagcctgc ttgatggggg ttttagcaat tatttaactg attttcttat atccttgctg
    11521 gctttcttgt gtagcatgat aagtttctac gtcaagaatg gcctgtttaa atttttctcg
    11581 ggtgatttca taaggcacat gtttaatatc atttgttgcc atggctttat ctaagattgc
    11641 ttctaaatcg gtcgtgacat tgagattcat gtcggttaac gatactggaa gttggttctt
    11701 tttcataaac acatagattt tatcaaacaa atcgaactgc tgatccatca tcaataaggt
    11761 cagtacacca taactgacag atgctccgtg aaggtatccc tccatttttt ctagaacagt
    11821 cgtaccgtaa tagaatgagt gtgccatagc agaattatag ttattgttaa ctaaaacaga
    11881 aaccaatccg gtagtaacaa tgatatccga aacgacttgt tcaattgcat atgagggacg
    11941 atcgtcggca ctatctttca atgcttgcgt accataagtc aagagacgat caatgcacat
    12001 ttttccgatt tggattccta atctatccgt gtagcctaga tcatcgccac gagcagaaaa
    12061 agtagattcg acttctttgg ataaagcatc gccaatacct gcccataagt atttatcagg
    12121 tgcttcggca ataattgctg tatcaatgaa ggtgtgtaca ggtggttttg aaggaaagaa
    12181 gagtccgtcc attacgcctt cttcgttata gatgacacat accgcagtga cgggcgaaca
    12241 gttcgaacca atagtgggaa aggtgaagac tggtttagct agtttttcac caatgacttt
    12301 tacagtatcg caagcacggc caccaccgac tgcaaacagg acgtctgctt tttggacact
    12361 ttcatgctgg atcaactgtt caatatttcc ataagttgaa ttacccccgt accagagact
    12421 ggcgacaacc tctatttcct gtgtcaaagc tacttttatt ttgttttctg cctttgaaag
    12481 tgcagtcttc cctccaatca gcactacttt tgtcccatat tctttgacga ttccatttgc
    12541 tttttcatac gcatctgttc caatggtata atttggtaag aaaacacttt gactcatatt
    12601 catttccttc tttcttcgtt gaaacgggtt aaaattttaa agagtgatcg atctgactgc
    12661 ggctgtagat ggagtagagg tgaacctaag acaggataag tgatgaataa ggtggcatga
    12721 acaccttgaa atcatcaata agtatcctaa aaacgaacaa gtacaaaaac attgaaaacg
    12781 acaataatga atttttgttc ctcttagtat ttctaccgat tccctttgct tttttgtaat
    12841 tatctaaaaa ttctaaaaat taagattatt atatgaaaac aggttttatt tgtcaattca
    12901 taattggttt tttttaaagc aaaagataat ctatatcatg atgaacaaac agagtttttt
    12961 cctccaattg atggctttcc tgatctttcc tattttgaat aaagtgtttc tgtataattt
    13021 ttttatttta taagtaagat aaataatgaa agaatagaga tactgattgg tagctaaaga
    13081 tttttgagcg agaacatttc gttttttcaa aaggactgtt agaataagca agaacaaggg
    13141 ggaagacgta tgataatcag tgaatttgac cgtaataatc cagtattgaa agatcagctt
    13201 tctgatttac tgagactgac ttggccggaa gaatatggag acagctcggc agaagaagta
    13261 gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg tagaccaaga tgagttagta
    13321 ggatttattg gtgcaatccc tcaatacggt atcacaggtt gggaattgca tccattagtt
    13381 gtagaaagct cccgacgaaa gaaccaaata ggtactcgat tagtcaatta cttagaaaaa
    13441 gaagtagctt ccagaggagg aatcacgatt tatttaggta cggatgattt agaccatgga
    13501 acaacgttaa gtcaaacgga cctgtatgag catacatttg ataaagtggc ttccatccag
    13561 aaccttcgtg aacatccgta tgaattctat gaaaaattag gttataaaat cgtaggtgtc
    13621 ttaccaaatg caaatggctg ggacaaaccg gatatttgga tggcaaaaac gattattcct
    13681 cgaccaaatt ctcaataata aaaagaggtt gtgacagaag taatcagctt caagaaataa
    13741 gaaggaattt acgaaaatta cttcttgaat ttttgtgaaa ttccaactta tttccgaaga
    13801 agctactttt ggaacaccgt gtaccagagg ttgtgaaaaa gtcgcttagt ttcgagtagt
    13861 aagaaaaatt ttgtgaaaat ggctttctac atttttgcat aaatttggct taataccgag
    13921 aaactggctt ttgaacaccg tttattcaga taagggactg tgacaagact tttgtcacag
    13981 tttctttttg tttattcttt tttatctttg tatggcaata atagacaata taacgaaaat
    14041 tatttaatgg cttttggaag accatggaaa accttgtaca aagctatttt ttctgaaaaa
    14101 ttccttgaaa cctcacgaaa aaatcattat tttatgaaaa agacaaaaaa aagtgttgat
    14161 tttttaataa ttattcatta aactcttaaa caacttcaga aaatattaat tattctgaaa
    14221 attccaataa aggggtacgt aaaatgaaaa agaaatttac atttggtttg gttgcggtat
    14281 gcggccttgt acttgctgga tgttatggcg gcagttcagc cacaagcagt agttcagcaa
    14341 aaagcggttc agcagatgga ggaggcgtat ttaacttagt cgtgcctcaa gaaatgccta
    14401 cagctgactt atcagttgca acagatacaa tcagttttac agcattgaat aacgtttatg
    14461 aaggaattta ccgtcttgat gaagacagca aaccacagcc ggcaggagct agcgagttgg
    14521 cagaagtcag tgaagacgga ttaacttaca aaatcaaatt acgagaagac gcaaaatggt
    14581 caaatggtga tcctgttaca gcagcggatt acgtttatgg ctggcaacga acagtagacc
    14641 cagctacagc ttctgaatat gcttacctgt atgcgccagt tgaaaatgca gaagacatta
    14701 cagctggaaa gaaagataaa tcagaactag ggatcaaagc agttggtgac tatgaattag
    14761 aaatcaaatt gacgaaacaa acaccatatt tccaatatct gcttgctttc ccatcatttt
    14821 tcccacaaaa acaatccgta gtggaagaaa atggggatgc ctatgcatct gcaagtgata
    14881 aagctgttta taatggacca ttcacattag aagattttga tggaccagga acagatacag
    14941 aatggactta taagaaaaat gatcattact gggacaaaga tacagtaaaa ctttctgaaa
    15001 tcaaagtcag tgtagtaaaa gaatcttcta ctgcattgaa cttattcaaa gatggccaag
    15061 cagatgacgt gatcttgtct ggtgagttag cacagcaaaa cgcaaacgat ccagcttata
    15121 cttctgttaa agaagcgcgg acaagctata tcgaattcaa tcaacgtgaa aagaattcac
    15181 cattcaacaa tgtgaaccta agaaaagcta tttcttattc tatcgatcgt gaagctttag
    15241 taaaacaagt actaggtgac ggatcagttg tctctacagg attgatccca tcagatatgg
    15301 taaaaagtcc aacaacgaac gaagactttg caaaagaagc tggaaaatta gtttcttatg
    15361 acaaagataa agcaaaagaa tattgggaaa aagcgaaaaa agaattgggc gttgattctt
    15421 tagaatttga tttgatggct tctgatgatg attcatcgaa aaaagtaatc gaatatgttc
    15481 aaaactcaat ccaagaaaac ttggatggcg taacagtcaa accaacacct gttccattct
    15541 ctgtacgtct tgaccgctca acttcgggtg attttgacgc agtactaggc ggatgggctg
    15601 ctgactatgc agacccaagc agctttactg atttgtttgt cacaggtaat tcatacaacc
    15661 gtggtcgctg gagcaatgca gaatatgacg aagcagttaa agcagcttca tcaacagacg
    15721 ctggcgatga acaagcccgt tgggatgatt tccaaaaagc aatcaagatc atctctgatg
    15781 atatgggtgt cgttccggtg taccaaaaag cagaaggtca cctagtgaat gaaaaaatca
    15841 aaggtatcgt tcaccatgcg gcaggcgctt catgggatta caaatggaca tacgttgaag
    15901 aataatcgtt aataccgtga gcatgagaaa aaacgaggct gcggcaaatg tcgcaacctc
    15961 gttctcatat tcacttatcc taacaaagaa aaaagcatga aaatagcaga aagagcaatt
    16021 agtattccgg caatgatcag ccaaaagctg tacataccgt agctggcaga cgagagcgag
    16081 gaaaattctg gttttttctt tcggttcctt gttcgtgcaa gatggaccga tcgctgaaaa
    16141 tgatcgaaga aacccgaaga aaagacccat aaaaaaccgc cgataatcag aaacggcaat
    16201 gaccataaga atagatcatt agataattga atgagtgtaa aggaatggtt gatgagattt
    16261 tttattaaaa taaccagaat cagtagaaaa gctactgcgt aggggataga tttttttttc
    16321 atttgattgc tcctttattt ttggttcatt atctaataat gtagcgaaac atctgtcaga
    16381 agtcaaaagc tagggggaaa cattagtgaa tagttatatc aaatatgtat taaaacgtgt
    16441 ctttttcatg atcatcacgc tttggttaat tgcaacaatc acctttttct tgatgcaatt
    16501 gctgccgggc acgccatata cgaatcagga acgtttgagt cctgaaacaa tcgagatgct
    16561 aaataaacaa gtcggtttgg ataaaccagt tatcgtacaa tatgggattt atctttctaa
    16621 tttgcttcaa ggagattttg gaatttcctt ccaatttaaa aaccagccag tcgcacattt
    16681 gttagctgga agaatcggac catccttgca gttagggtta caggcaatta tctttggaac
    16741 agtcctaggg acgattttag gaacgatctc tgccatgaag caaaatacat gggcagatac
    16801 atcttctaca ctcgtagcga tattaggtcg ttccattcca aactttgtat ttgctgtttt
    16861 attacaatat atctttgcaa tcaaattaca agttttgcca atcgcaaaat gggatggctt
    16921 tgtgtacaca atcttaccaa caattgcgtt ggccatgtcg ccgcttgctg actctgctcg
    16981 gtttatccgt acggaaatgg tggaagtact gcatagtgat tatgtggaat tagcaaaagc
    17041 aaaaggattg agccgttggg aaatcgcctt taaacatggt cttcgaaata gtttgattcc
    17101 attgatgact ttattaggac cgttagctgt agcattgatg accggatcac tggtggtaga
    17161 aaatatcttt gctattcctg gtatcgggga acaattcgtt aagtcaatca caacaaatga
    17221 ttatcctacg atcatggctg taacgatcct ttactcattt atgctgattt ttgttatttt
    17281 gatcgtggat ctattgtacg gtctagttga tccaagaatt cgtgtttcag aagggagccg
    17341 aggataaatg gaaatgatac caaagcgtta tgaaacagtt gcagatatcc ctgcggatga
    17401 atttcttcca ttacaaaaaa ataccgaaga agaacgtgaa cagattactg caccttcttt
    17461 gaattttatc caagattcat ggcgccgttt aaagaaaaac aaagctgccg ttatttcaat
    17521 ggttttacta ttggtcatca ttttgatctc tgtgatcaca atctttgttt ctcctcatga
    17581 tccaactgcc caaaacgtgg cttatatcaa tttgcctcct cgtattccgg gaatcaatat
    17641 cgatggattg aatggaaaag caatggttgg tggtgaactt gttgataaat atgctcaagc
    17701 aaacgtccct tcaaacgtaa atttcttttt aggaacagac ggtttgggac gtgatgtatt
    17761 aagccgctta tttatgggaa tgagaatctc gttgctgatc gcatttattg ctgcattact
    17821 agatgtaacg attggtgtag cttacgggtt ggtttctggt ttgttaggcg gacgtgtcga
    17881 taacgtgatg caacgttttt tggaaatctt atcaggaatt ccaaacctcg ttgtcatgat
    17941 tttgatgttg gtcgtttttg aaccaggaat tttttcaatc gtggctgcta tggccatcac
    18001 aaactggata ccaatggcac gtatcgttcg tgcacaaaca atgaaattaa aagatcaaga
    18061 atatgtttta gctggattga cattaggtga atcaagaatg aaaattgcat tcaaacatat
    18121 tttgccgaat atctctagtg tcattattat tcaaatgatg tttagtattc catctgctat
    18181 tttcttcgaa gcctttttaa gctttatcgg tttaggtctt acgcctccgt cagcttcatt
    18241 aggaacgatg ttgagcgaag ggtacaagac attcctgtat ctgccttatc tattatggat
    18301 tccggcagta actttatcgg ttatcatgat tggctttaac ttacttgctg atggattgcg
    18361 tgatgccttt gatcctaaaa tgaaagagtg aagacgatga cgaaaatttt agaagtgaaa
    18421 gacttagaaa tctcttttga tacgtatgct ggaaaggtac gagcgattcg cggtgtcaat
    18481 tttcatttga ataaaggaga aacattggcg atcgttgggg aatctggaag tggaaaatca
    18541 gtgacgacca gaagcatcat gcgcttgctg tcaagcaacg ccaatataga tgctggtcaa
    18601 atcctcttta aaggtaacga tatcgttgga aaatcagaaa gagagatgca aaaaatccgt
    18661 ggtaaagaaa tagcgatgat tttccaagat ccaatgacct ctttagatcc gacgatgccc
    18721 attgggaagc aagtcgctga gtcccttaga aagcataata aagtttcaaa aaaagaggga
    18781 caaaaagctg cattagaatt gcttaaatta gttggaatac ccgaagctga gaaacgtatc
    18841 aacagttatc cgcatcagtt ctcaggaggt cagcgtcaac ggatcgtgat cgcgattgca
    18901 ctggtatgtt atccggaaat cctgattgct gatgaaccta caacagcgct tgatgtgact
    18961 attcaagcac agattttgga gctgctgaaa gaaattcaga caaaaatcga tacatcaatt
    19021 atctttatca ctcatgacct tggtgtagta gcgaatgtcg ctgatcgtgt cgctgtcatg
    19081 tatggaggaa gaatcgttga agttggaaca tcggaagaaa ttttctacaa cccgcaacat
    19141 ccctatactt ggggactgct tggttcaatg ccgacattag atagtgcgaa cgatcgattg
    19201 tatgctattc ctggttctcc accggattta ttgaacccgc caaaaggaga cgcattctat
    19261 ccaagaaatg aattcgcgat gaaaatcgat gcagaacaag aaccaccatt ttttgaacta
    19321 tcaaaaacac acaaggcagc aacttggcta cttgctccgc aggcacccaa agtgacaccg
    19381 ccagaagaaa tccaacgtcg ctgggctatc tttaaagaga aacaaaaaca tacttacggt
    19441 caaggaggaa ttgctgatgc caaataaact acttgaagtt aaaggattga aacagtattt
    19501 caatgtagga aaaaagaatg aagtacatgc tgtaaatgac atcagctttc acatttatga
    19561 aggagaaaca ttcggtttag ttggagaatc tggaagcgga aaatccacta ctggccgtac
    19621 gatcattcgc ttgaatgaac ctactgatgg tgagatatta ttcgacggtc aagatataac
    19681 gaagatcaaa gataaaaaag gtttgacgaa attccggcat gatgttcaga tgatctttca
    19741 agatccatac gcttcgttga atcctcgtat gaaagtcaga gatatcattg ccgaaggaat
    19801 tgatgttaat ggactagcta aatcatctga agagcgtgca aaaaaagtcg atgatctatt
    19861 gagaactgtt ggattgaatc caagccacgg tactcgttat cctcatgaat tttcaggtgg
    19921 gcaacgccag cgtattggta ttgcacgagc attggcagta aaaccgcgtt ttatcatttg
    19981 tgatgaacca atttctgcac tagatgtgtc gatccaagcc caagtggtca atttgttgca
    20041 agatctccaa aaagaacatc aattgaccta tctatttatt gcacatgatt tgtcgatggt
    20101 taaacatatt agtgaccgaa tcggtgtaat gcataacggc ttgttattag agatgggaac
    20161 aagtgatgaa atctataatc acggtgttca tccttataca gaaagtttgt tatctgctat
    20221 tcctctacca gatccagacc atgagagaaa acgcagaaga atcaaatatc aacctgaacc
    20281 agatgacgga caagtacgtc aacttcgtga aatcgcacca gaacattttg tctatgcaac
    20341 tgagcaggaa gtcccatatt acgcaaaaaa actaaaacga caaaaggaat ctcttctggt
    20401 tgctaactag gagggacata gatgctaaaa gcttttaaat tccgtatgta tccgacagaa
    20461 gaacaaaaac agcaattgat tcgtactttt ggatgtgctc gatttaccta taaccatttg
    20521 ctgaaaaagc ggcagaaaag ttggcaacaa acaggagtag ccaatttttc gctcacaccc
    20581 gctacattaa aaaaagaata cccattctta aaagaagtag acagtcttgc tttagcaaat
    20641 gcacagctta atttggatcg ggcttttcgc aattatttca agggacgtgc cagcttccca
    20701 aagcttaaaa cgaaaaaaag tatgtggcaa tcttatacga ccaacaacca aacccgtacg
    20761 atctatctgg aaaatggcta cttaaagtta ccaaaacaaa aagaactaat caagataaat
    20821 cagcatcggc cagttgaagg atcgattcgc tccgctacga tttctgctcg atataatgaa
    20881 gaattttatg tcgcactact ttgtgatgtt tcgccagtaa aaaaagaatc tttagctaaa
    20941 tggatcggga tcgcctatca tccgaagaca ttgatccaaa catctcgacc tctagaagta
    21001 actttaccaa aatttcacca gacagaagaa aagctgcaac atgcacagcg gaaattgaat
    21061 gtgaaagtcc gctccgctca ccatagaaag atcaggttag atcaggcaag caattatcag
    21121 aaacaaaaga gaaaagtaat ggatctttat ctgaaacaaa agaaccagcg agaagattat
    21181 cttgaacaat tatctgggaa attggtcaaa caatatgact acttatttgt agaatctttt
    21241 ccaaaagaag aggcacatgc tgatttttct attcacgact ggcataaatt aataacaaaa
    21301 ttgcgttata agtcccaatg gtataataaa aaatttcttt tgatcaatac cgacggagca
    21361 gaagaaagca attccgtaag aaagagccaa gtagtagaaa aaatgggcag acattcggtt
    21421 atcaagggat aaaaaatgaa tgaaactgat aggagcgtta gggttagcct tgttaataaa
    21481 aagtcttaat gacttttgtt tctaggaatt tttcttttga agaaaagctc actaattatg
    21541 acaaaccaat aaaacaatag cagaaaaagt tcgtacgagc tttttctgct attgttaatt
    21601 ttttattcag aaagtttacg aatacatcaa aattctgaaa caaaaaacgt aactcacaaa
    21661 gagttgtttt tgcgtttttt cttgaatgat taggtctgaa tcttttgctc acaaccttat
    21721 tcatggtgcc ccggcttgag ctgatagatt tttgaaggac ggccgattcc tacaggacgt
    21781 tcaccgatct catcgaaata ttggagcata gctttcttaa aattggaatg atcgatcgtt
    21841 ttataatcga tccctaagaa tttagcaaat acttttcgag cttccgtaat cgtgaaatct
    21901 ttacctaaaa cttgtagaac ttgcggctcg tgttccattt tattgactac acggttgaat
    21961 gctttcaaga tgatttgact atggtcaaaa gccaaagtat ctttgccaga agactctcca
    22021 gtgactaaat caagcgagat agccacttct ccgctagata aatggatctt gttactatga
    22081 cgttctagtg tgaaccagcg tacttccttg gcatcatctc cggcgattag cggttcttct
    22141 ccaataaagg ctaagtagct gactgtgacg acccagcctc gcggatctcg attaggcgta
    22201 ctgaatgtat gcagttgctc aatattttct tttgaaataa ccacaccagt ttcttccttt
    22261 gtttctctta agacgctttc tccagttgac tcatcctttt ggacaaagcc accaggcaat
    22321 gcccatgaat ttcgataagg atgccctttt ctttggatca acaaaacttt cagctgatct
    22381 tcttctttgt tatagcacat cagtacgata tcgacagtca aagagggctt gtcatattct
    22441 ggacgttcct gtttttgata ccagttaaga aattcttctt cacttgcttt atgttcataa
    22501 tataattttt cttcttcttt agaagcaaaa cggttcatct gaccacctcc attgacagta
    22561 aaagtacttt ttaccttatt gtagcagact ctgaaaaaaa accaaggatt tacttgaaaa
    22621 atctaggtta tcatttaaat atgaaaaata aatggaagta tgagattgaa taaaaagaaa
    22681 agcagataaa acggcaatac tttataccgc tcttcacgaa tggcaaagaa gccacaaata
    22741 acgatatata cgtcaatgat aaaacgcaaa atccaagctg ctgtcataaa actgttcaaa
    22801 tgcccatgga ggttaaaata accatacata cgaatataaa aatagctgaa tattaaatgt
    22861 gctaatgaaa gaaaagcaat actaagtgta aaaaaaagat gtttcttctg catgattgtt
    22921 ccagtccctt actaagatag atagtcttta ttataccacg tgtgagggga gaaataaaaa
    22981 gccgtttttt agtagatatg aaacaaaaca aaaacccgca tttattcaac ctattcatcc
    23041 ttttaaagga tagattgaac gtagatagaa gtcgtttatc cagtcttttt cattttgaaa
    23101 aaaagctaac agcatgcttt ttttatggta tactagcaag gaaacgaatt cgcatcatgc
    23161 gataaaggag gaagagcccc gtggcagaaa aggaaacttt ttatatcact acgccaatct
    23221 attatccaag cggcaaactt catatcggca actcgtatac tacgattgca tgtgacgcaa
    23281 ttgctcgtta taaacggttg atgggctttg atgtgtttta tctcactgga gtggatgaac
    23341 atggtcaaaa aatcgaaaca aaagcagaag aactaggtgt aaaaccgcaa gaatatgtag
    23401 ataaaatggc ggctgatgtc aagaaattat ggaaaacgct tgatatcagt tatgataaat
    23461 tcattcgcac gacagatgat taccataaag cagctgttca aaaaatattt gatcgtttat
    23521 tagaacaggg agatatttac ttaggtgaat atgaaggctg gtactctgtt tctgacgaag
    23581 aattctttac agaaacacaa cttgcagaag tgtacaaaga tgacgaaggt aacgtgatcg
    23641 gtggtaaagc gccaagtgga cacgaagtag aattggtaaa agaagaatca tacttcttcc
    23701 gcatgagcaa atacgcagac cgtctattgg aatactatga agaacatccc gaattcatcc
    23761 agccagaatc tcgtaaaaac gaaatgatca acaactttat caaaccagga ttagaggatc
    23821 ttgcagtttc acgaacaact tttacttggg gagtaccagt caagagtgat ccgaaacatg
    23881 tggtgtatgt atggatcgat gcattatcca attatattac tgcactagga tatggctccg
    23941 aagatgaaag tctattcgaa aaatactggc ctgcagatgt tcagatggtc ggtaaagaaa
    24001 tcgttcgctt tcatacgatc tattggccaa tcatgctgat ggcccttgat ttgcctctac
    24061 ctaaaaaagt atttgggcat ggctggctat tgatgaaaga cggaaaaatg tctaaatcaa
    24121 aaggaaatgt cgtttatcct gaaatgcttg tggaacgtta tggtttagat gcactccgtt
    24181 attatttgct tcgtgctgtt ccgtttgggt cagatggtgt ctttacgcca gaagattttg
    24241 tttcccgtgt caattatgat ctggcaaatg atctagggaa cttattgaat cgaacgattg
    24301 ctatgatcaa caaatattgt gatggactcg ttcctgacta tgcctcactt gtcactccat
    24361 ttgatagtga actgtctacg acggctgcta acgttgtcgg acgttaccat gatgcaatgg
    24421 aaaaaatgga attcaataca gcattggctg aaatctgggt gctgatttct cgtgcaaaca
    24481 aatacattga tgaaacagaa ccatgggtat tagcaaaaga cgaagaaaag aaagctgaat
    24541 tgaatagtgt catgatccat ttagctgaat ctttgcgtat cgcagcgata ttacttcagc
    24601 caatcatgac agaaacgcca gagaagatct tcaatcaact aggattggac tctgaaacaa
    24661 tgaatcttga aggattacac tttggcgaat ttccttcagg aacaaaagta gttgcaaaag
    24721 gtacaccaat cttcccacgt ttagatatgg aagaagaagt tgcttttatt caagaaaaaa
    24781 tgtctgaagg tactcaaaca aacgaagata cagtcaaatg ggatccagaa gaaacagaac
    24841 ttgtttcaac gaaagaaaaa caaatcaaat ttgatgtatt tgaaaaagta gaactaaaag
    24901 tagcagaagt catcaactgt caaaaagtag aaggggcaga taagttgctg caattccgac
    24961 tagatgctgg agacagccaa gaccgtcaaa tcttgtcagg aatcgctgaa ttttatcctg
    25021 atccaagcga attgatcggg aaaaaagtcg tcattgtagc aaatctgaaa ccacgaaaga
    25081 tgcgtggaca aatcagtcaa ggaatgatcc tttctgcaga agcaccagat ggctctttac
    25141 aagtaatcga agcaccaaaa tccatgccaa atggctcaga aatcgcatag aaattcaaag
    25201 ggggtatgac actagttgtg tcataccccc tttttccgaa atttgagaag caattttcgt
    25261 aaattccttc ttattacttg aagccaatca tataatacag aaacagagaa ctatccaaaa
    25321 taaatcctag aaaaagaagt acgagcttcg ctgatttttg ttaatttaga ataatactaa
    25381 taggaatcgt aaaaacagaa aaaaagcata atgataaagg gtcgggcaga ttttaattga
    25441 ttcatctggt actctttttt attgtcctta cattcataaa gtttgaatgc aatgacttta
    25501 ttacatctat atttatatct aacagtagcg ttgatgtgtt aattataagt tctatcactt
    25561 tatgttcaat tagtttgtgc tcttatatgt gtgattgaaa atatagataa aaaaacaatc
    25621 atcggctttg acttcgatga ttgttttctt tattttgtaa aaatcctatc acttgatttt
    25681 atggtaacct tttatttttg tcttcaaacg gcttggtaag agagcagtaa gcagtgcaaa
    25741 tgggatcgaa aagacatacc agaaaatcgt acgtccgata ccataatgtt gatagagctt
    25801 tgtcaacatc acgtgtaaca agatcaaccc taaaagcaag gagccagcta taaaccaatg
    25861 attatcccaa tgcatgactt cactaagtgc catcactcca aaaggataga aataaaaata
    25921 gatcccgact aaggtcatcc gccagttctt tgtaaatgtt ccgatattca ataaaaagaa
    25981 aatcgctgaa agcaaaatac cgataggcgg aaaaagccca accaaaaagg aatacaacat
    26041 cccccacaag atcatcacag gacctttgat caatgcaacg attgcgatga acaaagtcag
    26101 caatattagt ttttgtgata acgttgcaac agtcattaat ccaaataaaa ttaatgtgaa
    26161 ccaatgccaa tgggactgct gttgttcttt acgggaaaaa tcgacccggt tggataaata
    26221 atctgctaat ttcggataag aacgcttaaa atccataaac tgttcctcct tcgtggaaaa
    26281 ggtaggttta gtatacgaaa aataaaaaag aaatacatcc gtcttaagag ggagtttttc
    26341 actgaaagtg gagaaaaaag aatggtaaat taaaatattc tcagttatac tgaaaattat
    26401 accaaatttc aaagaagaaa gaggatggga atgaaaggca aactaaaacg agaaatcaat
    26461 ctgtttggcg cattagctac cgttatggga accgtgatcg gtgcaggcgt cttcttcaaa
    26521 actgctgctg ttactgctag tactcagtct acaagtttga cattgctagc ttggcttttg
    26581 ggtggtttct taacgatctg tgctggattg acagtggctg aattagctac agcaatcccg
    26641 gaaactggtg gagctgtaaa atatattgaa gctgcgtatg gaaaattgcc cagcttttta
    26701 ttaggatggg cgcaaagctt gatttacttt cctgctaata ttgctgcatt atcgattatc
    26761 tttgcgacac aattgacgaa cttattacaa ttatctaccg attatctttt gttgattgca
    26821 gtgatcaccg ctgtttctgt gacagggttg aatttgttag ggacaaaagt cggtgcttct
    26881 gtacaatcag tgacgttgat tgttaaatta ataccaattg ctgtgattgt catatgggga
    26941 ttattgaccc caggacaagg aactgtccag ttatttccaa tcgaagctgg aaaagatgtt
    27001 acttttgtcg aaggattgag cagtgcgttg ttagcaacgt tgtttgctta tgacggctgg
    27061 ttaggtgtcg gtgcaatggc aggggaaatg aaacggccgg aaaaagattt gcctaaagca
    27121 attattcttg gtttgagttt tgtaacagtc gtttatctat tgatcaattt tgtttttttg
    27181 aaaactttgc cgattgatca tcttgccggt aatttgaatg cagcttctga agcatcagac
    27241 gtcatttttg gaggaatcgg tggaaagctg gtcactattg ggatcttgat ctcggtatac
    27301 ggtgcattga acggttatac actaactggg atccgcgtgc cttatgcgat ggctttagaa
    27361 gacgagctac ctttcagtaa acagctgaca aagctttcaa agaagtttac ggttccttat
    27421 gttcctgcgg ttttccagtt agcagtagct tgtattatga tgagccttgg ttctttcgac
    27481 tttttaacag acatgttgat ttttgtgatg tggctattca gtttactgat ttttatcggt
    27541 gtctttattt taagaaaaaa agcgccggaa ttgccacgtc catataaagt accactttat
    27601 ccaatcgttc cgattattgc gattctcggt gctattttca ttttaggtat gacgatgatg
    27661 acgcaaacga aattagcatt gatcggtata ggtgttacct tgataggtat cccagtctat
    27721 tatcaaaaaa agaaaaaacg ctcagaagag taaaatattt caacaacaga cagaaaaagt
    27781 acgaactagg tcctctagat cttcgtgctt tttctatttc aataaaacaa gttcttctat
    27841 tttgattttt gctacttctg caagtagtgc ttatgtgcta aagtaagaat gaaaaggaga
    27901 gataaacatg atatttgatt ctcataccca tttaaatgct gaacagttca atgaagatat
    27961 tccagaaacg attcagcgag cacaagaaat gggggtaacg aaaatggcgg tcgtgggctt
    28021 cgatacgcca acgattgaaa agtctttgca attaagccat gactatccaa atatatacag
    28081 tattatcggc tggcatccga cagaggctgg cagttatacg aaagaaatcg aaaataaatt
    28141 acaagaacaa ttgactttac caaaggttgt agcattaggg gaaattggct tggattatca
    28201 ttggatggaa gacccgaaag aagtccaaga taaagtattt cgcagacaga ttgctatcgc
    28261 aaaagaaatg aacctaccaa tcagtattca tactagagaa gcacttgaag acacgtatca
    28321 aatcctaaaa gaagaagatg ttcgagatat cggtggaatc atgcatagtt tcagtggtga
    28381 ttatgaatgg gcaaaacgat ttcttgactt agggatgcat atttcattta gcggtgtcgt
    28441 cactttcaaa aaagcattag acgtccaaga ggctgcagca aatgtaccaa tcgaacgatt
    28501 acttgtggaa acagatgccc cttatctggc accagttcct tttagaggaa agcgaaatga
    28561 accaggctat actcgttata cagtagaaaa gattgctgaa cttcggaatc ttccgacaga
    28621 agaagtggct gcccaaacat ggacaaatgc gcaccgatta tttggtctaa gcgaacatgg
    28681 ataagatgaa gatccaagag attatagtcg ttgaaggaaa agacgataca agaaggctgc
    28741 gggaagtagt tgatgcagat acgatagaaa caatagggtc tgcaatcaat gaagagatcc
    28801 tgatgcaaat tgaacatgcc caagagactc gtggagtgat cgtttttacc gatcctgatt
    28861 tttcaggaga aaaaataaga aaaatcatta tggaagctgt tcctcaggca aaacatgctt
    28921 ttttgccgcg cacacaagct aaaccaaaga aaaagggaag ccttggtgtc gagcatgcaa
    28981 gtgatgctgc tattttagaa gcattgaaaa aggtcgtgac gccagacatt tcccaaacgg
    29041 ctgtttctga aatcactaga caagaccttt tagactatgg tttaatagct ggggcacaag
    29101 caaaagaaaa acgagaaaaa ctaggtgacg agttgcgcat cggttacacg aacggcaaac
    29161 aattggaaaa acgtttgaag atgtttcgta ttacgccgaa agaactcgcc caagcaatga
    29221 aaatagtgga ggaacaacat gagtgaatat cgtgaaattg cgacaccctc tagaacaaaa
    29281 gagattttat tgaaacatgg tttttcattt aaaaaaagtt taggacaaaa ttttctgact
    29341 gaaccaaata ttttaaggaa aatcgtagaa actgcgcaaa tagatgaaca tacgaacgtg
    29401 atcgaagttg gaccaggaat cggtgcattg acggaacaat tagccaagca tgcaaaacaa
    29461 gtcgtagcat ttgagatcga tgatcgtttg atccctgtat tggctgatac gatgcaaccc
    29521 tatgacaatg tgactattgt acatcaagat attttaaaga ccgatttgtc aacagctgtt
    29581 agagaaacat ttcatgagga gcttccgtta aaagtcgtag cgaatttacc ttactatatt
    29641 acgacaccga tcatgatgca ttttcttgaa tcagatttgg ttgtggatga actagtcgtc
    29701 atgatgcaaa aagaagtagc tgatcgtatc tctgctgaac caggaacgaa agcttatggt
    29761 tcattatcga ttgctgttca atattatatg gaagccagtt tagcattcat cgtaccaaaa
    29821 acagtatttg tccctcagcc aaatgtagat tcagcgattt tgaaactgac aagaagagat
    29881 ataccggcag tcgaagtgac agacgaaaaa gcatttttcc gattgacaaa agcggcattt
    29941 cagcaaagaa gaaaaacgct ttggaataac ttgcagcatt cgtatggcaa agatgaccag
    30001 acaaaagcat ggttagcaaa aagcttggaa acagctggaa tcgatccaaa acgtcgtggg
    30061 gagactttgt cattacaaga attcgccgca ttaagcaatg cgatgtcaga aaatatgcag
    30121 taaaaaaaga ggttgtgaca gaagttgtga aaaagctgtc ctgcatcaag taataagaac
    30181 agccaaacaa aaatagttcc tcatgttttt cgtttgtttg ggcttgttac cgcagatgca
    30241 agcttttgaa caccgtttaa gaaagaggtt gtgggcctgc cgtttaactc taagtcacaa
    30301 ggaacaattt caaaaaacag cttctcatgt ttttgagtta gttggtctta ttacttgatg
    30361 caagcttttg accactatta tgccgaacaa ggagatgtga cacaaatttt gtcacatctc
    30421 ctatttttgt ttcttgatca aaaaattctt atacttgata ctcacactca ttttgtatgg
    30481 atttagaaaa gaatatgtgt cttgacagtt attagtaagt gagttatact aagtcgacat
    30541 ttatgtgtca agacatatta ggagagagaa aaaatgagaa aaaattcagt tcgaaaacta
    30601 acgatttctg ctttattgat tgggatgggg gtcattatcc caatggtcat gcctaaaatc
    30661 atgattccac cagcttcctt tactttggcg tctcatgtgc cattatttat tgcgatgttt
    30721 tttacaccag gagttgcagt ggctgttgcg ctaggtacga catttggttt ctttttaaca
    30781 acgccggtta tcatcgcatt gcgagcattg tcacatcttg tgtttgcgtt gattggggct
    30841 tggtacttgc aaaagcatcc gagtatcgtt ttaaaaaatg gacagtttac ttttgctaat
    30901 tggcgtttcc aagggttcaa ttttgtgatc ggtgtgattc attctttagc agaaatgcta
    30961 gtagtcagca tcttttactt tattggtaat atgcctgaaa catattattc tcaaggatat
    31021 ttttatacag tattcatctt gatggggatt ggtgggctta tccacagttt agttgattat
    31081 aatattgctt attttatagc aggaacactc agcaaatatt ttgatatccc cgtatttacg
    31141 gcagcaaaaa aagaaaatcg gaaagagtcg attcaagggt acgcgaagaa aacgacatat
    31201 tagacatgat ctctcaaaaa aaaaactctc atctagtaat ggataataaa tcctctaaga
    31261 tgagagtttt ttgtcctaaa aagagctgca ccaacatctt ctggcacagc tctaacaggg
    31321 ggcaatttat ggaagtttga tgacttgttc tgtttcttta tcaaagaagt ggcctttgct
    31381 gatgttgaag gcaaggtcga ctatttcgcc tggtttatgg aaatctcttg catctacttt
    31441 tgagatgaac tcagtagaac cagtttttgt gtaaagcatc gtttcagctc caagtaattc
    31501 ggaaacgaca acttctgatt tcactacggc ttcaggtgaa gcttcaaggg ctacttgttc
    31561 actgtggatg tcttctggac gaataccgaa gataacttct tttccttcat aacctttttc
    31621 aactaatact ttgttttttc cttcaggaat acgaagctta agcccatgtc catctgtgat
    31681 aacaccattt ttcaatgtca cattaaagaa gttcatggca ggagatccaa taaagccggc
    31741 aacgaacata ttgacgggtg tatcataaac ttctttcggt gtaccgattt gttggataaa
    31801 tccgtctttc atgataacga ttcggtcagc catagtcatt gcttctgtct gatcgtgagt
    31861 aacatagatg gtcgttgttt caagacgctg gtgaagtttg gcaatctcag cacgcatggc
    31921 tacacgtaat ttagcatcta aatttgataa tggctcatcc attaagaaga cttttgcatc
    31981 ccgaacgatt gcccgtccca atgccacacg ttggcgttgt ccaccagata aagcagctgg
    32041 ttttcgtttc aagtattctg tcagacctaa gatctctgct gcattgtcca cacgtttttt
    32101 gatgtctgct ttatcatatt tacgtaattt caagccaaaa gccatattat caaaaacagt
    32161 catatgagga tacaatgcgt agttttggaa aaccatggca atatcacggt ctttaggtgc
    32221 cacgtcattc atgacttttc cgccgatatt tagttctcct tcagagatat cttccagtcc
    32281 cgcgatcatt cgcaaagtag ttgactttcc acaaccggaa ggaccaacaa aaacgataaa
    32341 ttcacggtct ttgattgata gattaaaatc agttacagaa taattttctg cattgtcata
    32401 ttttttatat acgtttttca gagccatttc taccataata cgttccacac tttctttttt
    32461 agttttatta actatttaaa gtataaatga aagcgttaac taacttcaat gtcatgttga
    32521 acaagaattt tttctttttt ttgtcaatgt gctagtttta agctttaata aatatccata
    32581 agaaataaaa actgatacac tggagtaaat aagtatgtta acaatatcaa aaggagtgtc
    32641 tcttatgaaa atcgctttga tcgctcatga ccgaaaaaaa cctatgatgg tgaaattagc
    32701 aacagcgtat aaaacaatat tggaaaaaca tgaacttttt gctaccggga cgactgggca
    32761 gaggatcacg gaagcaaccg gcttgccgat ccatcggttt aaatccggac cgttaggcgg
    32821 ggatcagcaa attggagcca tgatttcgga ggatcagtta gatttggtga tttttcttcg
    32881 agacccgctg gctgcacagc cccatgaacc agatgtaaca gcacttatcc gtttatctga
    32941 tgtctatgag attccgttag ctacgaatat cggaacagct gaggtgctac taagaggact
    33001 ggaagccggc ttcctagatt ttcgaagaat cgtccatgaa atagacaagc atccactttc
    33061 attttgatgg ataagcgtat ggaaaaataa cgtttaatgt tcgcctatat gtagctataa
    33121 aagctaaaca aactgataat atgcgatata atacgaacgt attggataat ataaacaata
    33181 atattagttt ttcgtatttg cagtaaatgt gaaaatatgc tatagtttct gtgggaaaag
    33241 atcccaaaaa taatacagag tagaaaataa agcgttaagt gctaagagga tgggatgttg
    33301 cctcttggac gaaggacgta atgttcgcgg ttttattttc gcattcgctg catgatacga
    33361 tgtagcagct cttaaaggag atgctaaaaa tgaagaaaca ccggttagac gcacgtatga
    33421 ttgctattat gggcttattg attgcattga tggtcacttt gtcccgactg gtagcaatcg
    33481 agaccccatt tattaaaatc agtgtcacat ttattccaca agtgatcatg ggcattcttt
    33541 ttggtccttt ttggtcaggg ataggcgcag ttttagctga tttagtagga atggccctct
    33601 tctcgaaatc tgcattcttt attggattta cattgaatgc atttattgaa ggagcaatct
    33661 acggattctt tttctatcga aaagagatca cttggaaaaa tgccattctg gctacgctta
    33721 gtgtcacgct gatcatcaac ttatttttga caccattatg gcttgcgttg atgtaccatg
    33781 tgccattgtt cagctgggtc gtttgggcgc cccgcctgtt aaaaacagta atatggttac
    33841 cgattcaatc aattgccatt tattacgtag gacgtagtat tccttacaaa aaaattttaa
    33901 gaagtttagc gatccacgct aaataaaaaa caagcagtct tcccatccca gaagactgct
    33961 tgtttttatt gtgctgtgaa ttgtgcttga tagagccgtc ggtaataacc atctttttga
    34021 agcagctcat catgggtgcc catttcgaca atagctccct gatccatcac taatatctgg
    34081 tccgcatttt tgattgtgga caggcgatgg gcaatcacaa aactggtttt tccttccatc
    34141 attgctaaga aggcttgttg gatttttttc tctgtcaatg tatcgactga gctggtagct
    34201 tcatctaaga tcaacatagg gggattactg atcatcgttc gagcaatcgt caacagttgg
    34261 cgttgtccat ctgatatctt taacccttga ccgccaatct tagtatctaa tttttccggc
    34321 aaacgttcta caaattcata catataagag gcttttaggg catcattgat ttctgcgtca
    34381 cttgcttccg gattcccaaa acgaagattt tctcgcaatg tactgtcaaa tagccaagta
    34441 tcttgtaaca ccattccaaa acttttacgt aatgtatcac gttggataga gcggatgtca
    34501 tggtcatcga ctgtaattgt tccttttgtt gtgtcataaa aacgcatcag caaattaaca
    34561 agtgtggatt ttccggcccc tgtttttcct actatagcaa tggtttgacc tggataagca
    34621 gtaaaattga aatcttcgat caaacgctgc ttggggtcat aagaaaaaga aacatgatca
    34681 aaagagactt ttcctttgat atttttgggt tccaaggcat aggcatctgg caaatcaggc
    34741 ttttcttctg gctgatcgat caaatcaaat gctcgttcta gaccagaaaa agcggtttgc
    34801 agttgtgtcg tgattccaga aagttcgata aatggtttag aaaactgact tgagtagatc
    34861 gtaaagctgg aaatcacacc aacagtgatt gacgaaccac tcttcaatgc caataggccg
    34921 ccaactaaac caatcgataa ataagcaagg tgatctacaa aacgagaaga cgggtttgtc
    34981 aaggaagaag aaaattgtgc ccgttgtcct ttttcatata gatcagcatt tagcagttca
    35041 aattgctgtt gactagcttc ttcttgctga aaagctttca cgattttctg gttaccgatt
    35101 cgttcggtca caaagcccga gatatcccca ataatccttt gctgtctatc gaaatttgtt
    35161 tgtgaagcac gagcaacgat ccaactcaca aggaaaataa ttggtgtaga acaaagaacg
    35221 accaatgtca gaagtggact tagacgcatc ataaaaataa acgaaagaat gatgattgcc
    35281 actccagaaa ataattgatt gaaggccgca gaaattgcaa tggaaatatt gtccatatca
    35341 tttgtaaatc gactgacgat atttccgtgg gagttttgat cataataacg aagtggcaat
    35401 cgattcagat gagcaaatgc atcttttctt aattttgcaa cggataagta agctaaccga
    35461 ttgcttaaca cttggatgag ccattgactg actacgttga ttaaaacgat accaccgaat
    35521 agaataagta aatggaacag tcgattgaag cggacctgtc cttctccaat catttgatcg
    35581 atactttgac caatttgtaa cgtcatcaca actgttgcaa tcccattgat gattcctagt
    35641 atcacagcta gccaaatgtc ttttgcatag gcagtaaggt aaggaatgaa tcggcggagc
    35701 gtctgcaagg ataatttttt tactttcata actattcttc ctcctgtgat gcgacgattt
    35761 cctgatattc tgctgattga gcaagtaatt cttcatgggt accctgagca gcaatcttac
    35821 cttgatccat cactaagata tgatgtgctt cttgaataga gcggacacgt tgggagataa
    35881 taatcactgt actgccaaga taagtctgta gcgccttacg caaatccaag tctgtttgat
    35941 aatccaacgc acttaaagag tcatctaaaa tcagtaatgc tggcttggca atcaaagctc
    36001 ttgcaatcgt cagtcgctgt ttttgtccgc cagagaaatt tttcccattt tccatgactt
    36061 ttgtatctaa accatttgga agctgacgga caaattcttc tgcctgagcg attttcagag
    36121 cttcccaaca ttctttttct gtggcgtctt tcttacccca ttgaaggttt tcacgaatag
    36181 tcccggtaaa tagtacagaa gtttggggaa caagagcaat ttgctgacgg agatgatcca
    36241 gctgccaaga acgaacatcc attccgtcca ttataactgt accggaactg acatcataga
    36301 aacgcggaat caattgtgtc aaagtcgttt tcccgcttcc ggttggacca atgattccaa
    36361 ggactgtatt tcgcttcagc tgaaatgtaa tattttggag agaaagacca gaatcaggag
    36421 tataacggaa gtcaacatgg tcaaaagcga taattgcttc agaagaaaaa tcgggagtag
    36481 tactgacctc agcttcaaga atcgaatttt ctgtgtctag cacctctttc acccgatttc
    36541 ctgaagcttc ggcacgtgtg aagatcacga cgaggtttga aacaacgatc agtgccaaaa
    36601 gcatttgatt catataattg atcaaagcaa gaacttgtcc ttgttgcaag ctaccaatat
    36661 ttactttgat cccgccaact gtcagcaaac agatgatccc gacattcatg atcaaggttg
    36721 tagcaggtga taatagggca gagatattgg aaacacgtaa ataattgttc gctagatcat
    36781 ttgtgctttt atcgaacttc tctgtttctt tttttgtctg cgcaaatgcg cggatgacac
    36841 gaacaccgct aagattctga ctaacgtgtt cattgagctg atctagtttt tcttgcactt
    36901 tttggtagag aggaacggtt tttttgatga taaaaaataa aatgattgag aaaataggca
    36961 ataatgctaa gaaaaacaga cctacttccc agtcgatcac aaaagccatg accactgacc
    37021 cgatacttaa aaacggtgct ctgatcaata aacgaatgac cattgctaaa gctagctgaa
    37081 gctgattgat gtcgtttgtc attcgggtga tcaaggtatc tgttccaaaa tgattcaact
    37141 cgttgtgaga gaaagtattg atttttttga ttagctgatt acgtaactct gtaccaaatc
    37201 cttgtgaagc tactgaagca tagtattggc aaatgatagc acaaatcaga ccaatcacag
    37261 acatagcgac catccataag cccatttcaa tgatttttcc tcggtcattc atttttagtc
    37321 cattatcgat caaggaagcc atcattaaag gcaggacaag ttcgaagaca gcttcaagaa
    37381 atttgaagaa cgggcccaaa atgatttgct tacgatagtc ttttgcataa cgtaataact
    37441 gaatcatatg attcctccta ttatttattt gtccacaatt gaaaaaacaa gtactaccac
    37501 attgtagctt aggaaattta gaatgaaaag agaacaagcc ttttcataaa tgaaagtttt
    37561 attatgaaga agctttagaa attgaataga ctattttttt taaaaaaaag aaaatgagaa
    37621 aacatgccag tctcttcatt cataggaacg tctattttac ttaatttttc tataaatgaa
    37681 ttctgcataa aaaatatatc aagtaaatgt ttatagatga aactctattt acttcttttt
    37741 taatttgttc ttgcaatctt ttttgctacg taatttttgt aaaaaagtgg gttgagagaa
    37801 atctaattga ttttcaaaga aggagtatga aaatgagaaa aatatttatt cgtttattgt
    37861 gggtactatc ttagaggaat aaataatagt ggagaaaact atggtttggc gcagattcta
    37921 tcaggtttga tggaacgaag acaacctccc gacatcaagt caaaagctac ttgagatttt
    37981 gatctgaaaa taaaaaaaga aaacaaggta ggaacatcaa tgtttctacc ttgttttcgt
    38041 aatcaatggg aaaaaactaa gagtggtgta cgtaatcaat aaaaaggatg gtgacaacag
    38101 tacctcttta ctctctgaaa gcatcataac tcgtttttcc tcattatgcc aataaattgt
    38161 tgttatacca catataagga tttgtaatag ctaaaaacag acaggaaagt tgaaatctgt
    38221 gaatcttatt ttgtatggaa ggatttatag aacgcaaaaa tcaagataaa gatgaataaa
    38281 aatcctgttt cggctatata ttgcgtatct acgccaataa aacgagtttg ttctgtcata
    38341 gccatataaa gcaaaggcaa aaatacaact acagcgagat aagatccgat gatttctcga
    38401 aaagttttcc aggaagtatg tttcctgttt ttatatttat ccgatttcat tgaatagtca
    38461 tagcctcctt attctttcat atgttgaaat tccagtataa aaactggaag gaactgtacg
    38521 ttttttagaa taaaaattat aagggataag taggctgctg tctataataa gatatatttt
    38581 tcaattcttt attaaagatt ttattgacaa aatgaatccg ttttctatga tggaggaggg
    38641 gtgaataagt tgttgaagaa ggaaatttta tgttacttag agaaccagac agcctttttc
    38701 gatttagaac atatgaacga ggtctttaca gcacaaaggt tagctgatca ttttggggta
    38761 aaaagaaaca cagtcagtca ttatttgaac cagttaaatg atgagcaact actagtcaaa
    38821 atcaattctc gaccagtgat ttatttccac aaagaagctt ttgaaaaaca attttttaag
    38881 ttacgcacga atttttattt ttctatcgat gatctcaaag aggaacagcc ttttttcgcg
    38941 caaccaaaag acttattctc ttttatgatc ggatacgacg ccagtttgaa agaaagtatc
    39001 gaacaaatca aaacagctct ttactatcca gatggcggat taccactttt gattacaggc
    39061 gaaagtggca caggtaaaag ttatttagtt aatttggtct atcagtattg tctgcttcat
    39121 gatctgctag aagattctgc accattcatc acttttaatt gtgcacaata tgcagataat
    39181 cctgaacttt tgactagcaa tttgtttgga catgttaagg gagcgtacac tggtgcagaa
    39241 gaaaataaaa aaggggcttt tgaagcagca gatggtggga tcttgttttt agacgaggta
    39301 catcgcttaa caccagaagg tcaggaaaaa ctgttcacct atcttgatca aggtgtgatt
    39361 tatcgtatgg gagaacctaa tttgtcacgc cggatcaaaa cacgtctctt ttttgctacg
    39421 actgaagaga ttactagtca tttcttaaca actttcattc gaagaatacc tatccgaatc
    39481 gaactacctg cacttaatca gcgtagtcgt aatgaacggc tagaactgat ttactctttt
    39541 tttctcgaag aacaaagaaa aatggggagc cctttgtcag tcagtggacc agctttgtca
    39601 cttttaacaa gtcagaaatt atccggaaac ataggagagc tgaaaaatat cgtcaaagtt
    39661 tcagcagcaa aagcatatgc tgaacagcgt gaacagcctg aaatccatgt cacgatccat
    39721 catttgcaaa aagaactact tgcgcagcct attactaaaa atacagcaca gcaaggaatc
    39781 tatattacgc aagaccagac cttagagcaa ttaatggaga agcaacagcc cgaacagcag
    39841 cgaatcgtta aaagttttga gcaaatccta attgatttca agaaaaataa ctgtctgcta
    39901 tattcttgcg aaggaaaatt aaagcaagaa gtgattcaat tatttgattt tttgctgttt
    39961 gagaccagta gacaagaaaa acacgagctg ctggtttatt ggacacaatc tatccgagag
    40021 acctttcgac aaatggaaac tgcctatcaa atcaaattcg atggaaacag tgtctatgcc
    40081 ttgagttact atctctatca gcgaagcact atcaaatggt taccggaaga acatgatatt
    40141 atccaactaa tcaaagaatt agaacaacaa gtttctcagt cttatccatc tagttatcac
    40201 tatgtccaac gaattctgga attaagtaag atcaaattag atattgaaga cacaagtatg
    40261 gatcggattt tactgacgat ctatctaaaa aaagcgaaat ggtcaaaaga aaaacgactg
    40321 ccaaaagcga tgattgctgc tcacggttat gcaactgcta gcagtatggc aaatgtagta
    40381 aatcgtttat tacaagagga tctatttgaa tcatttgata tgccactaga tgtcacacca
    40441 cagcagattg cttcagaaat ccttgattat tgcgaaaaca gcgatgtttc aaatggattg
    40501 atcatattag tagacatggg atctctgaaa gaaatccatc agttttttaa aaaacagtta
    40561 tccgtaccgc ttttgatttt gaataatgtg acgacacctt tagcaatcac agtaggggaa
    40621 tgcctgcaaa aaaatgtatc attagaagag atagcagaag agactgttcg acagatccag
    40681 ccagaatggc gactgctcta tccagaggaa aacaaaccca aagcactcat tactacttgc
    40741 ttcactggaa tcggtacggc ggtccactta agtgaactgc tagaaaaaag cttacctacg
    40801 gcttgtcagt tgaaaatcat cccatatgaa tatcagcaat taaaagataa gaaaaacagt
    40861 gacccattat tttccatata cgaagtcgta ggaatgattg gcaccacaga tcctgatatc
    40921 accaatatcg actatctttc attagaagac ctcatctcgg gggaaaagat gtctgtacta
    40981 gctgaatggt tggacactac tatgaatgct tcagagaagg aaatattcaa tcagcgcatc
    41041 attcgtaatt tttcattgga aaaagtactg gattccgtga ctattctaga tacagagaag
    41101 gtgatgggag aaatcgatca ttttatgaga gaattagagg tgcagttgaa tcgaagcatc
    41161 agcaatccca aaaaattagc gttgtatgtc catgtgagct gtctcattga acgattgatc
    41221 cgacaaatgc caatcgaaac ctatcaagga ttagagaaat ggaagcagtg tcaaaaagaa
    41281 gggttatctg ctattaaatc tgcgtttagt gtcattgaag agcattatag tgtcatgatc
    41341 cccaattctg agattgccta tatctatgat attttgagtg aagatacaga gtttctctcg
    41401 atagaagaag aattttgaac ataagcaagt tgttgccact aattttggca tgcaacttgc
    41461 ttttttagta tgtgtaagtg aaaagaggag atgaaaaagt ggatagagaa attatcttag
    41521 cttcccatgg aaaatttgct tcagggatat tagattcatt agagctgatc tatgggaaaa
    41581 atcattctat tactgtattc gattgctata ctgatgagaa ttttgattta gcggaaacag
    41641 taaggcagct ttttatgagg tacaaagata aagaaatcat tgttctgacg gatctttttg
    41701 gcggaagtgt caacaacgaa ttcttacaat atatcaatcg ggatcatgtc tatctcgttg
    41761 ccggattgaa tttgccacta ttgattgagc tagtatctcg gttggacgct gaagtagaga
    41821 caactgtttt gatccagcag gtgctaactg attcaaaaaa aatgatccag ttttgtaacg
    41881 agcggctcaa tcagaaaata gaagaagacg aattttagga ggaagaaaaa tgattgtatt
    41941 gacaagagtc gaccatcggt tattacacgg acaagtagct ttttcatgga cacaaacaat
    42001 aggcgcagac tgtattttga ttgctaatga tgatgtacct acgaatgaga tacgaaagac
    42061 aacgatcaaa ttagccaagc cacaaggagt caaactagtg atcaaatcaa ttgatgactc
    42121 gattgccgcg ttgaaaagcg gggtgacaga taaatataag ttattcatcg tagtggaatc
    42181 gatcgaggat gcatataagc tggcagaagc gtatccagaa atcaaaacca tcaacttagg
    42241 agggatcaag gcaaaacctg gtactcgcag catcggaaaa gcggtaaata ttttaccaga
    42301 agaggaggaa cgcttgaaac gattaattga caaacaaata gaagtagaaa tccgtcaagt
    42361 accagcagat aaaaaagtgg atgtaagaga agttttgtaa gaaaggaaga acctcaatga
    42421 ttgtacaagc agtcttatta ggaattgttg catttgtcgc acaatcagaa tatgcactag
    42481 ggacatcgtt gctttctcgt ccgattgtga cggggctgtt tacaggaatc gtgttaggtg
    42541 atgtaaaaac tgggatcatt atgggcgcaa ctttagagtt agcatttatc ggatcgttct
    42601 cagtaggtgc ttcgattcca cctgatgttg tgaccggtgg gattttaggg acggcttttg
    42661 ctattacagc tggagccggt acagaaaccg cgcttttact aggtttgccg attgctacgt
    42721 tgacattgat attgaaaaat atttacttag ggcttttgat tccaattatg aaccataaag
    42781 cagacaatta tgcagaagaa ggaaattata aaggagtcga gcatatgcac ttgcttgccg
    42841 gtatcggtct ttccttgatg cttggtgttg tggtaatggt ttcttatatg gtgggaagca
    42901 atacgatcgg aaatctatta aatatgattc ctgacttcgt tcaaaagggg ttgtcagtag
    42961 ctacagggtt gattccagct cttggttttg cgatgcttgc tcgtttgctg atcaacaaac
    43021 aagttgcacc atattttttc ttaggttttg ctatagcttc ttacttagag attcctgtga
    43081 ccggagtagc tatttttggt gcgattttag cagttgtggt agtcaatatt atgaatgtcc
    43141 gtcaactacc acttcaaaca actgcagggg aggtagaaga agatgaagaa ttctgagatc
    43201 aaaaacagca aaataacgaa aaaagagcta aatcacgtat tttggcgttc ttttcaaatg
    43261 gagttttctt ggaattatga acgtcagatg aatatggctt atgtttatgc aatgattccg
    43321 attttgaaaa agttatatca gtcgaaagaa gaaatggcag cagctttgaa acggcatcta
    43381 gagtttttta acacgacccc acatatcgtt acactgattt taggaatcac ggctgcaatg
    43441 gaggaagaaa ataaggatga tcctgaattt gatgtgactg cgatcgatag tatcaaaaca
    43501 tctttgatgg gacctcttgc agggttagga gattctttct tttggggaac tttgcgattg
    43561 attgccacag gtgtgggtac ctctttagcc atgcaaggaa atattttagg ccctatcttg
    43621 ttcattttga ttttcaatat tccgcacatt ctgtttcgct atctagcaac aggttgggga
    43681 tatcgcttag gaacgggttt tttgaaaaag atccaggcaa acggtatgat ggaaagtttg
    43741 acattaggcg cgtctattat tggactgatg gtcgttggag caatgactgc cacgatgatc
    43801 accatcaata ttcctttaaa aatcggttca ggagaaaatg ctgtgactgt tcaaagtatt
    43861 tttgatgata tcgttccaaa tatcttatgt cttggagcat ttggcatagt gttttatcta
    43921 ttgaagaaag aagtgaaacc attaacgatc ttgatcggac tagctttctt tggaattttt
    43981 ggttcgtgga tagggatttt ttaaaaaagg agcagagaaa atgtttacga tgcaagatta
    44041 tatctatgaa gaaaaggaag tactaagtac gatcttgaaa aagaatgatt tttcgacaag
    44101 agaacatatg aaaaaaacag tgaatttact gatcttagca acgggttcat cttataacgc
    44161 ttgtttagca gcgaagccag cattggaatc ttatggtgat cttactgtcg atattcaaga
    44221 acctttttat tttttcaatt atggaaaact ttctccttca atcgatactg tgattgctgt
    44281 gtcacaaagc gggaaaagtg cttctactgt tgaagcagta aaaatgatac aaaaacaagg
    44341 gttacctgtt gttgctatca caaatgacgt gcaaagtcca cttgctttag aagctgcaca
    44401 aattattgat ttaggtgccg gggtagaatc agtaggcttc gttacaaaag gctattctgc
    44461 gactgtttta cagttactac tcttagggat aggaattgga ataagtaaaa ataaaatcag
    44521 caaaaaaata gaacaagact acatgcaaca gttgcgcaaa ataatcaacc atttaccagc
    44581 gattattcaa aaaacggaag aattttttga tggatatcaa agtttatttc gtttagcaca
    44641 gcgtttcgtc accattggat atggtccaaa ctggggaaca gctaaagaag cagagacgaa
    44701 attgactgaa acaatccgcg ttccgtcaca aggatttgag ttagaagctt atatgcacgg
    44761 cccatacctg gaggcagatg cttcgcattt gctattcttt attgaagggg aaagtgtaaa
    44821 taaggaacgt tcgcaaaagt tgcaaagata tatgagtcgg tacgttggag agactctcac
    44881 cattaccaca aaaaaagcaa gaaacgagaa aacacttgga ttagcaatag aatgtgatga
    44941 atatctgtct gtattagcgc ttgtcgttcc gtttcaacta ttcgcttata aaaccgcagt
    45001 agctaaaggt attgacttga acaaaaagat ttttgaagac tttgatacag tcttgaaaag
    45061 taaattataa gaggtggaaa tcatgctgaa attcaatgaa gaagaacaaa tcaaagcaat
    45121 aaaaggcgca ctagctttac gtccgcaagt agaagaaatc atcgataaac tttatgcgga
    45181 aaaatttgac gctgtctatt atctaggaat cggtggaacg tatgcttcct cgatgcaagc
    45241 tgtcacttat atgaatggga aaagcaactt gcctgtcttt gtacagcatg ctgcagaata
    45301 ttacactact ggaaacaaaa gattaacaaa agattctatc gttattttgt cctctgttac
    45361 cggtacaact caagaagtag tgaaagcagt agaacaaatc aagaaagtag gagcgacact
    45421 gattggattt atcgataaag cgaacagtaa gttgtctcag ctatgcgatt ttgtggtcac
    45481 ttatccagct cctggaacag aacaaatcaa attttttatg gcagcagatc gattgatgta
    45541 tttgaatgga gaattcgaag cttattcaga atactatgaa cagctcgaac gttatttacc
    45601 aacagggtta gtggaagcag agaaaaaagc agatgcattt ggcttatcat ttgcagaaaa
    45661 acatcgacgt gatagtatgc attattttat tggagcaggg aatcaatggg gagctgttta
    45721 ttcatatgcc atgtgttact gggaagaaca aagctggctt ccatcgaaat ctatccatgc
    45781 tgccgagttt cttcacggga cgttagagat cgtagaagaa acaaccccag tcacattatt
    45841 tttaggcgaa gatgaacaga gagtgttatc tgaacgtgtg gctaaacttt tgccaaaaat
    45901 ttgcagcaat tatacattga tagacacaaa agactatcca gtagaaggta tcagtgaaaa
    45961 atatcgcgga cgtgttttgt ctttcttatt gatgcatgta gtgacacaac gcatcgatgc
    46021 tcatgtggag cagttgaact gccatccatt agagatacgc cgctactatc gtcagtttga
    46081 ttattaggat agactggata aaaagaatat aaaaaataaa gagagcagcg ataactgctc
    46141 cctttatttt tttaaaatat tggcatcaat tacgttataa aacacatttt ttgcattatc
    46201 tacagttcaa cttataatct gtttaatggg acatgtgggt gccaaccatt ttttgttatg
    46261 caatagtact atttagttgt tgcatggatc cgtagtaaaa gtccaagtac aacgagttgc
    46321 gccagtttac atagcgtttt gtatccagaa tgttgactaa aacaaaagtt cctatgtcaa
    46381 ccgtcaacag agacaatctg tccgttgcag gacctgacag atttagaaat gttcaatctg
    46441 tctcttatgt attttaaatc aatactcact aggagaagtt tcctaacctc ctaatagtaa
    46501 tgctggtagt aacattgtca aaactgcttc ctcttaggaa caagcaaagc ataacgagtg
    46561 taaaaataaa atgtagacta ttgtaacata tcattgatta tttcaagtaa aaacgataaa
    46621 agaagtaaac aaacacttaa aaacaatgga aattagtccc aactttaaga aatgtatact
    46681 ataatggaaa ttatcaatta tttatttgga ggaatatcca tgaaacaaaa aatcgcagtt
    46741 cttggtcctg gatcttgggg cactgcttta gcacaaacat tggcagaaaa cgggcatgat
    46801 gtccgtattt ggggaaatgt acctgaacag attgatgaaa tcaataagta tcatacaaat
    46861 cagcattttt tacctgattt gacgatccca gaatcgatta tcggatataa agaattggca
    46921 gaagcagtgg ataacgcaga tgctatttta tttgttgttc caacaaaagc gattcgttca
    46981 gtcgcacagg aacttattgg taaaatgaat actaagccaa tcattataca tgcaagtaaa
    47041 ggactagaac aagatacgca taaacgaatc tcagaagttt tggctgaaga aattccagaa
    47101 gaaaaaagac aggcaattgt tgtattatct ggtcctagcc atgctgagga agtggcagta
    47161 catgatatca caacaattac agctgcaagt attgatcaaa aagcagcagc ctatgtacaa
    47221 aaactgttca tgaatgatta tttcaggatt tatacaaata atgatgtgat tggcgtagaa
    47281 actggtgcag cactgaaaaa tatcattgca attggtgccg gcgctatcca tggattagga
    47341 ttcggagatg atgcaaaagc agcaatcatg actcgaggat tggcagaaat cagccgtctt
    47401 ggtgtcgcga tgggtgccaa tcctctgaca tttatcggtt tgagtggtgt gggggacttg
    47461 atcgttactt gtacaagtgt tcattctcgc aattggcgtg ccggtaactt gttaggacag
    47521 ggacataagc tggaagaagt tctagaaaac atgggaatgg tcgttgaggg tgtggcaacg
    47581 acaaaagcag cagtagagct agctcaacag ctaggtgttg agatgccaat tactcaaaca
    47641 atctataatg ttttgtataa tggagaagat atcaaaaaag cagcaaaaga aatcatgtta
    47701 cgtgacggaa aaatggagaa tgaattttct cttcgttaaa tagatgatca atagttaatc
    47761 gt
//
LOCUS       DAJSAW010000018        47011 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJSAW010000018 DAJSAW010000000
VERSION     DAJSAW010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.018"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(152..1126)
                     /locus_tag="ONT64_001102"
     CDS             complement(152..1126)
                     /locus_tag="ONT64_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HCR2302534.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(1107..2990)
                     /locus_tag="ONT64_001103"
     CDS             complement(1107..2990)
                     /locus_tag="ONT64_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HCR2302535.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(3008..5233)
                     /locus_tag="ONT64_001104"
     CDS             complement(3008..5233)
                     /locus_tag="ONT64_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR2302536.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5348..6382
                     /locus_tag="ONT64_001105"
     CDS             5348..6382
                     /locus_tag="ONT64_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2302537.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(6449..7462)
                     /locus_tag="ONT64_001106"
     CDS             complement(6449..7462)
                     /locus_tag="ONT64_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2302538.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(7540..7728)
                     /locus_tag="ONT64_001107"
     CDS             complement(7540..7728)
                     /locus_tag="ONT64_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR2302539.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(8168..8296)
                     /locus_tag="ONT64_001108"
     CDS             complement(8168..8296)
                     /locus_tag="ONT64_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR2302540.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(8299..9672)
                     /gene="rlmD"
                     /locus_tag="ONT64_001109"
     CDS             complement(8299..9672)
                     /gene="rlmD"
                     /locus_tag="ONT64_001109"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR2302541.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(9783..10838)
                     /locus_tag="ONT64_001110"
     CDS             complement(9783..10838)
                     /locus_tag="ONT64_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR2302542.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(10848..12278)
                     /gene="gatB"
                     /locus_tag="ONT64_001111"
     CDS             complement(10848..12278)
                     /gene="gatB"
                     /locus_tag="ONT64_001111"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCR2302543.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(12275..13747)
                     /gene="gatA"
                     /locus_tag="ONT64_001112"
     CDS             complement(12275..13747)
                     /gene="gatA"
                     /locus_tag="ONT64_001112"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCR2302544.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(13747..14052)
                     /gene="gatC"
                     /locus_tag="ONT64_001113"
     CDS             complement(13747..14052)
                     /gene="gatC"
                     /locus_tag="ONT64_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCR2302545.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(14158..16194)
                     /gene="ligA"
                     /locus_tag="ONT64_001114"
     CDS             complement(14158..16194)
                     /gene="ligA"
                     /locus_tag="ONT64_001114"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR2302546.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(16211..18445)
                     /gene="pcrA"
                     /locus_tag="ONT64_001115"
     CDS             complement(16211..18445)
                     /gene="pcrA"
                     /locus_tag="ONT64_001115"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCR2302547.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            18600..20159
                     /locus_tag="ONT64_001116"
     CDS             18600..20159
                     /locus_tag="ONT64_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HCR2302548.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(20270..22201)
                     /locus_tag="ONT64_001117"
     CDS             complement(20270..22201)
                     /locus_tag="ONT64_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2302549.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(22219..23133)
                     /gene="pfkB"
                     /locus_tag="ONT64_001118"
     CDS             complement(22219..23133)
                     /gene="pfkB"
                     /locus_tag="ONT64_001118"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR2302550.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(23130..23882)
                     /locus_tag="ONT64_001119"
     CDS             complement(23130..23882)
                     /locus_tag="ONT64_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR2302551.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(24041..24250)
                     /locus_tag="ONT64_001120"
     CDS             complement(24041..24250)
                     /locus_tag="ONT64_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302552.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(24261..25589)
                     /locus_tag="ONT64_001121"
     CDS             complement(24261..25589)
                     /locus_tag="ONT64_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302553.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            25725..25880
                     /locus_tag="ONT64_001122"
     CDS             25725..25880
                     /locus_tag="ONT64_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302554.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(25954..26541)
                     /locus_tag="ONT64_001123"
     CDS             complement(25954..26541)
                     /locus_tag="ONT64_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR2302555.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(26624..27874)
                     /gene="clpX"
                     /locus_tag="ONT64_001124"
     CDS             complement(26624..27874)
                     /gene="clpX"
                     /locus_tag="ONT64_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCR2302556.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(28121..29410)
                     /locus_tag="ONT64_001125"
     CDS             complement(28121..29410)
                     /locus_tag="ONT64_001125"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR2302557.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(29564..30496)
                     /locus_tag="ONT64_001126"
     CDS             complement(29564..30496)
                     /locus_tag="ONT64_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302558.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(30653..31312)
                     /locus_tag="ONT64_001127"
     CDS             complement(30653..31312)
                     /locus_tag="ONT64_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR2302559.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(31315..32340)
                     /gene="sppA"
                     /locus_tag="ONT64_001128"
     CDS             complement(31315..32340)
                     /gene="sppA"
                     /locus_tag="ONT64_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR2302560.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(32353..32643)
                     /locus_tag="ONT64_001129"
     CDS             complement(32353..32643)
                     /locus_tag="ONT64_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302561.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            33052..34857
                     /gene="glmS"
                     /locus_tag="ONT64_001130"
     CDS             33052..34857
                     /gene="glmS"
                     /locus_tag="ONT64_001130"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR2302562.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(34929..36284)
                     /locus_tag="ONT64_001131"
     CDS             complement(34929..36284)
                     /locus_tag="ONT64_001131"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR2302563.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(36308..37477)
                     /locus_tag="ONT64_001132"
     CDS             complement(36308..37477)
                     /locus_tag="ONT64_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302564.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(37474..38361)
                     /locus_tag="ONT64_001133"
     CDS             complement(37474..38361)
                     /locus_tag="ONT64_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCR2302565.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(38550..42242)
                     /gene="nifJ"
                     /locus_tag="ONT64_001134"
     CDS             complement(38550..42242)
                     /gene="nifJ"
                     /locus_tag="ONT64_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR2302566.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            42441..42962
                     /locus_tag="ONT64_001135"
     CDS             42441..42962
                     /locus_tag="ONT64_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCR2302567.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(43661..44218)
                     /locus_tag="ONT64_001136"
     CDS             complement(43661..44218)
                     /locus_tag="ONT64_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302568.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(44397..45008)
                     /locus_tag="ONT64_001137"
     CDS             complement(44397..45008)
                     /locus_tag="ONT64_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302569.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(45121..45378)
                     /locus_tag="ONT64_001138"
     CDS             complement(45121..45378)
                     /locus_tag="ONT64_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302570.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(45975..46253)
                     /locus_tag="ONT64_001139"
     CDS             complement(45975..46253)
                     /locus_tag="ONT64_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302571.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(46287..46538)
                     /locus_tag="ONT64_001140"
     CDS             complement(46287..46538)
                     /locus_tag="ONT64_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302572.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT        12931 a        10106 c         8300 g        15674 t
ORIGIN      
        1 tgtttttcag aaaaataaca attgatttta ttgaagaaaa tagtcatgaa caaaagtatc
       61 tgagaacttt tttactctct tgttcatgac atatagaata aagcagcttt gtcttgtaaa
      121 ctccgttttt caaataataa tctaaagatt gctactgaat aataaagccg atttcatggt
      181 cctcttcgct gctgattcgg taaggtgact caacatccgc tccccagcta ttgatacccc
      241 cgactcctct tacttttgca aaaatcgtaa gaaccgttcg tctttctggc ggtagttctt
      301 ctatatgtgt tgcctgttcc agttcttctg ctttgaatgg taaagcggaa aaagcaaatg
      361 gcgtatcctg ctgttcaaat cgcaacgaaa aatcttcttg atctttatcc gtattatcca
      421 acgtttgatt tcttgttagt tctacccatt tcgtttccat atgcatgccg cattcttggg
      481 gaacaaggta tggggaaacg gggagtcctg ccactttata aataccttcg acaccaccag
      541 ccatccgatc cggatatgtt tcccctgata ggccttcata ctcaaaaaat tttgctttcg
      601 ttggcataat gaaccttaaa cccaatgcgg gtaattccgg taatcctttt tgtccataat
      661 aatgcatatt gacaagcatt ttaccctctc cagcgatttg gtaggaaatt gtcacttggg
      721 tttttggtac tgtgttgatt tccagtgtaa aaatgatttc aaccgtttca gcgtattcat
      781 tacttgaata ttgattattt actggagcaa tcggcaaatc gatcttttta ttgtccacca
      841 gtacttctat ttctttgact tttggataca atccggctcc taaccacata ccggatttaa
      901 atccaaatcc gctacctctg tcattatcgg tcgttgctcg ccaaaaaact ggaagtgttt
      961 cccggtatag ccattctttt ccgtttttat tgagcgattc cagtccgcct cttgtataag
     1021 aaaaaatata atgcgtattc cccgatttga cacctaatgt acagtcgccg aagacgattg
     1081 caactttttc cgtatccata tgacttttat ttactgtatt tttcataata atatcttacc
     1141 tcctgcatgg cgggtttttc ttgtccatct gcaaaaacga tcccattccc tgagaattca
     1201 tagtcacttg gtttatcgtc aaagtcaccg ccatagcgca tcacttctct gcctgtgatt
     1261 ggatcagtca cttggatcgc ctgatcttta aaatcccata tatagcctcc ttggtacatt
     1321 tcataacgat caagcaacgt catgtaagaa tccattccac ctaacgaatt ccccatgtca
     1381 tgcatatatt cacataagat gaacggtttt tccgctttat tttcaaggta gtcaatgatc
     1441 tcttctggtg gcgcatacat gcagcttttt acatctgaaa tcgaatcttc gaatgctgga
     1501 tttggaaaat tcccttcata atggacaagt cgatcggggt ctactttttt gaagtaatcg
     1561 ttcatttgtc gaatgttttc ttccgcataa gattcatttc caagagacca aaaaagaata
     1621 cttggatggt tttttaatgt ttcgaaattc gtttttgctc gatcaagaac agccgcctgc
     1681 cactgaggta ctgcacctgg tacgttgtaa gaaggctcaa atgcgcccat tttttgccat
     1741 gagccatggg attcgaggtt cgtttccgcc atcacatgga ttcctgcttg gtcgcataaa
     1801 taataccaag tgatctggtt ggggtaatga gatgtccgta cgccgttgat ctttgcattt
     1861 tttatgatag ccaaatcttt ttccatatct tctatagtga tggcgcgtcc ttttttcgga
     1921 tgccactcat gtcgatttac cccgttgata atcatccgtt ttccattgaa gtagatgatt
     1981 ttatctttga tttcgatttt acgaaaaccg aaggaatagt caatatactc cgttactctt
     2041 ttttcttcgt ctatcgtctc caattcaagc ttatataaat tgggctgaaa atgcccccac
     2101 agtgccacat tttcaaatcc atcgatgcga atcgtcattt gctcttttaa cggatattgc
     2161 tgttgataga tttccttccc atcaggatct agtatcgcta cattcagctg actgtttttg
     2221 gctacttgct caaaagacaa atctacttca agtttgccat tttgacattt gtcatctaag
     2281 cgaggtttga tccaaagatc ttctaaatgg ttttgtcctc gagcaactaa gaagacatct
     2341 ctgaagatcc caaaaaatcg gaaaaagtcc tgatcttcca agaatgcagc tgtacttctt
     2401 ttgtaaacct ccacagctag aatattttct tcttccaaat aaggtgttaa atcaaattca
     2461 gacggagtga acgaatcttc ggcatacccg ataaaatgac cattcaacca aataaacatt
     2521 gcttgttcta caccagcaaa atgaatcgag atttgttttc cctttagacc ttcttctaat
     2581 cgaaaagtcg tcaaatacga accaaccgga ttgtctttag cagctgaaaa gatcccttcc
     2641 gcctctttct cagggcttgt cgtgaaaggg ggacggcgaa atacatgtcc ctcccacgga
     2701 tacatcgtat tgatgtaatg gatcttgtca taatttgcta attcaatatg actaggaaca
     2761 gtgatttcat caaaagaaca ggcatcaaaa tcttttttat aaaatgtttt tggtcgtacc
     2821 gaaggatttg gcgaataaca aaaacgccat gatccattta aagactgttt aagggaggat
     2881 gtggtttcta cttcttccct tgttgcataa caaacatgat cgctatgtgc atttgattgg
     2941 ttcactctaa aaacttgtgg atcatctaac cattttatat ttgctttcat acttttttcc
     3001 ttctctctca taactttttg aaatgaagca gttctgtttg gaaatcacct tgaaaatcgg
     3061 ggaagtaaag tccaactgtc atcaattcgg cccccgaaaa tatccgcttc gaggtctgtt
     3121 cctgataaag tgcctcatca tctaacccca tcagatatac ttcatggaac gctggttgag
     3181 ctgaagctag tatcctccct aaaaacacaa tagcttcatc ttgtttagga gacacaaaca
     3241 tccatgcggc aatattttct tcaaaaggac tcgccaaacg ataataatgg ccatactgga
     3301 tcaccggacg aatcgtttga taggatataa tttggtcttt tacttgcttt ttttcttcag
     3361 gagacagctt tgtcaaatcc agttcataac caaaaacagc gctcatagct gtttctcctc
     3421 ttgttttcaa cgaagtagat ctgtcggtct gatgatttgg tacagctgat acatgagctg
     3481 tgatggacga aatcggataa gcaaggcttg ttccatattg gattttcata cgctccactg
     3541 catccgtgtt atcacttgtc catgactgcg gcatataata aataaacccg gcatcaaacc
     3601 gtccacctcc accagaacag ccttcccata agatttccgg ataatcagtt gtcaaatgtt
     3661 caagtagttc ataaagcccg agcatataac gatgcgctac ttctccttgt ctctgtgggt
     3721 caagggtaat cgaatagaca tctgaaagcg aacggttcat atcccatttg atgtagtcaa
     3781 gaggaatcgt atctaaaatc tttctcattt gtttctcaat cgtctgccga acatctaacc
     3841 ttgtaaaatc cagtacatgt tgtgatctgg aagcagaagg cattctacct ggctcctgca
     3901 taagaaaatc aggatgtgtt cgatacagtt cagaatcaac cgagatcatt tcaggttcaa
     3961 accataaacc aaatttcaat cctttgctat gagcataatc tgctatttca cgtaatccat
     4021 ttgtcaactt gccttcatac tcgaaccagt ctcctaatga agaattgtcc gcatctcttt
     4081 ttccaaacca gccatcatct aatacgaaca tttcgatgcc tagctccttt gcttcatcaa
     4141 tgatcgcctt gattttttca ctattgaagt caaagtaagt ggcttcccag ttattgacca
     4201 gaatcggccg ttcagcaaat tgatgacgac ctcttgcgat tctttcacgt agcaactcat
     4261 ggtggatctg actcattttg ttcaatccct gacttgagta agaaaggatc atttcaggtg
     4321 tttgaaaaga ctggcctgca ttcaattccc acgaaaaacg atggctgttg atcccaccga
     4381 cgactctcaa ttgattgatc tgatcttttt caagttcgaa agaatggttg ccggaataaa
     4441 caaattgaag tccgatcgct tctccagtat tttctgtcgt atgatgatga actaaagcaa
     4501 taaagttatt catatggtga ctgctcgttc ctcgacggct ttcaaatact tttgttccag
     4561 ataaaacaga ttgccgtgaa atctgtcttt cattgacatg tgctcctggc aaagcaatga
     4621 cttcatcaaa tcgcctagta tgggcaaagt ctagctggaa actagccgct ttttctagtt
     4681 tgattgtttc tccggtttca tttcttatct ttacagatcg tgtgatcact gctctatcag
     4741 taaatatggt atagaacaaa tcgaaataaa tcgctgtttc tctatcctta agtgaaatca
     4801 ccaacgtttg tgcctctgac ttgtccatca cataagtttg aggcaagcct tcgatctctg
     4861 gcttcccatc tgtaatataa tggctgtcat atctcagatc cagtaagtct gatccattct
     4921 tgcgctgaat gatcgatgcg ggcgttcgaa agtccatgct gccatgagaa gagtattctt
     4981 gcggtagtgt atctttcgac agagaccggt caggatttaa gggaacattt cctgaaaatc
     5041 cacgatctct tcggggataa tttttattgt ctttgtactg cttgactggt ttgccaaaat
     5101 aaatatgcgc caatacattg ctttcttcta tctttatcag ataagaaaga aatgtatttc
     5161 gtaaatggaa gactcggctc ttttcatcaa ataagatgaa atttttatct gtttttctat
     5221 gcgtaatatc catcatttca ctcccaacaa aacggaaacc gtttactttt ttttataatt
     5281 cctgtatact gtttactaaa caattttgtc aataaaaatt ttttattttt ttgaggagtg
     5341 tgtaaaaatg gttggaatta gagatgtcgc acgaaaggca aatgtctctc ctgctacagt
     5401 ttcccgtgtg ttgaataacg atcagacgat ccatgtggct cagactactc ggcaacggat
     5461 agtagaggca gctgctgcgt tggattatga catcagtact cgtaaatata tcaaaaagag
     5521 aatgccttcg attggtgtga tctctacgat cggcaaagcg agcgaagaac aggatatcta
     5581 ttacaaacat ttgcgagtag ggatggaaga agaagctcga cgtctgcatt tagggatgaa
     5641 tcgaatctat aatctatctg ataatcctaa acaatgggaa gatctagatc agttaggtgc
     5701 ggtgatcgtt gttgggactg ttacgaaaga atcgattacc gccttgttaa aacaaaacat
     5761 gcaaatagtc gtcgtggata accctgacat ccagcaagag gtcgatatgg tgtatgcaga
     5821 tcttgaacgg atgactaaaa ccgtactgga attgttctta gaaaatggtc ataaaagaat
     5881 agcctatatc gggggatata aagtcgatat ggatgaattc ggtgggaagc agatttctat
     5941 aaatgaaaag aggatgcgtg cctatcagca ttttatgatc gatcatcact tagaaaaata
     6001 tatgtcttat catctaggtg gctggacaga aaaacaaggt gaggtacttg cgactaatct
     6061 attagaaaat gaatcccctc ttcctactgc cattcttgtc gggagcgatc caatggcaat
     6121 tggtgtctat cgggcattga aacaaaaaaa tatccaaatt ggagaagaga ttgttgtagc
     6181 aagctttgat aatatcgaga caatcgcaaa cctctcccct ggcttgacca gtgttcaaat
     6241 caatgcgaaa tcaattgggc aagcagctgt tcgaatggca ttggagcgaa ttgacggtat
     6301 acgggacgaa ccaatcattt tgacttatcc tgcacgattg attgttaggg aaagttttat
     6361 cccaagaaca aaaaggtatt gaaagagagg ttgtgaaaag ccgcttcgtt tcttcatact
     6421 caaaacgaaa cggctttttc acaacctctt acttatactt attcacaaaa tccaatgtct
     6481 gctggtaaca attttgcgat tcttttagcg tgtaatcaaa ttggtattca tgggtaatct
     6541 ctttttttgt atctgtataa aacaaggatt gcacgggtac ctttaattct gtcaaccgat
     6601 tttctaatgc ttgaccttgc tcctggaatg agtatgcatt tccgtcagtt atatacgttg
     6661 gcggaaattc cttagaaaca tggtcagcca cactagcttc tttcaattgc tcactcgttt
     6721 tccaatcttt tgttccaatc aacgaccacg caacggtttt gacgaaaaac ttcataaatc
     6781 gatcatccga atgctgctgt gctgttagct tcaaatcaac aggtccgcaa taagaaatgg
     6841 ttcctttcag actatctttt ggaagtagct gtttgatttt catttgttta gcatacgcat
     6901 cattcgtttg tatagctgca tattgcaatg cgatttgtga acctgcacta tctccaccaa
     6961 aaaataattg ttccatgtct agcaatttgt cgtctttttc ttcagctaat aaatctttga
     7021 tcagttcatt cacttgaaga acttgattag ggtattctga atcaggcgct aattcataat
     7081 tcatcgcaac cactgcgatg ttggcgtctg aaactaattt tgtcgcaaat tctttgacac
     7141 ccgatttatc tccgccgacg aatcctccac catgtaccca aaatagaaca ggaacaggcc
     7201 ccttgctgtt ttttggatag tagatgtcat aagtattctc tttatacttg gactcatacg
     7261 tcaaatctgt ttttttcgcc acgttttttt ctgcttgatc gaacgttttt ttatcagtga
     7321 tttgaacagt atttgaaaac atatggttga tcaagaatgc tccaggtctg ggggaaagct
     7381 ggaaggcaag aaagattgca aacgcgatcc caagaatgat tccgaagaac catccgataa
     7441 atttaagaat tttctttttc ataaggctcc ctcagtagtt agtattaagc attctgatta
     7501 taccatagtg ttatttccta tcaagctctc ctagatgatt taatgatttt ccccaaactt
     7561 cttctgcaat gcttttgaca aaagaaattt tttccattgt tcttcaaaca aaacattttc
     7621 ttcttctgtt gaagcaaatc cacattgagg acttaagcac aaacgatcca aaggcagata
     7681 tcgtgtcgct tcgtagatcc ggtcaatcac ttgctggtgc tcttccattt cgccattttt
     7741 cgttacagtc aaaatctaag acaactaatt tatcttcaga gactttctct aaaagaacaa
     7801 aactcccagc acgttcacta tcgtattcta aataataggc acggacattt tcttgagtaa
     7861 aataatgggt ctgctacgtc gtcatccccc ctgatgccgc caaaatcgaa taataatttc
     7921 cgcgtcaaac atgtatattt agtgttgaat catttgagaa tatgaaaata aaaatctttt
     7981 tgatcaatca gttaatcaaa ttgaaataac gaaaaatctg agacagaatg acgttcattc
     8041 tgacccagat ttgattttgc acaactcaac agtccttatt ggcaaatcgc caaaataatg
     8101 tagactaact aaaaagacta agaaacaata acaaaagtta acacttctta gtctttcttt
     8161 agcacgtcta tttattaaag attttagggc cttttctcat gtttttacga tcattcttag
     8221 gtgtttccat ttgtttggat cggcctccac cttgtttctt tttgatcaat tctttcattt
     8281 tttctttttg gtccattttt attctccttt aacgaatgaa gcgactaatt cgatatgatg
     8341 ggtttgcggg aaaagatcta caggctgaac tttcttcacg gcataacctg cttctgcaaa
     8401 taacttagca tctcttgcaa aagttgccgg attacatgag atatagacga ttcgttctgg
     8461 tgacatttcg attgctgatt caataaaatg atgatctaat ccttttcgtg gagggtcaac
     8521 tacgatcaca gatggtttga tccctttttc ttgccatttt ttcataactt tttctgcttt
     8581 tccgacttca taatgaacat tttcgatttt attatttaaa gcattgaact gtgcatcttc
     8641 aattgcttct ggaataactt ccatcccata caccttatcc actctgtctg ctaaagacaa
     8701 gccaatcgta cctattcctg aataagcgtc gatcacaatg tcttttttat ctaactcggc
     8761 aaactcgatg gctgtttggt acagatactc tgcttgttga gtatttactt gataaaatga
     8821 ctttgctgag attcgatagg tttttcctaa tagctgatct tcgatatagt ctctgccata
     8881 taaaactttt tctttgtctc ctaaaatcac atttgttttt tcttcattga tattttggat
     8941 gaccgaaacc acttctggca attcttcatg aatagcttca gcaatttcct tggatttaaa
     9001 gaatttttct ttacgagtaa ctaaaacgac catcatttca tgggaataat gaccacgacg
     9061 gataatgatg tgacgaagct gtccttcatt tttttcttca ttatatcctc gtacttggaa
     9121 ccgttgcaag atatctctga ctactttgat tgcttgatcg atgacaggat cttggatata
     9181 aaaatcttcg atagggacta gatcatggct attctttttg taaaaaccgg tggctaactg
     9241 tccatcgata cggcgaacag ggatctgtgc tttattacgg tagccgaatg gttctgccat
     9301 tccgaccgtt ggaagtacag gaacttctgg catcttagca atcttcgcca ttacatgctc
     9361 tacttgtttt tgtttgaaga caagctgttg ttcatacttc aaatgggcta aaggtgcgat
     9421 acctatgcga agtaatgcat tgttttctac aggctgacga tcaggtgatt gtgtaataaa
     9481 tgtttcgact tttccatatc caaacttatt gcctgctttt actactagta tttctacttc
     9541 ttctccaggg agtgcatttt cgataaataa tggataacca tcgatttttg ctacccccat
     9601 tccttcataa ctcaagtcga cgatttcgac tgtatatcgt tcgtttttct ttactatttg
     9661 ctgttctgtc atattttgct cctttaagcc tatttttcaa tcctactgaa ctctactata
     9721 aatcagctaa ttacacaact aaaaccggca ctgaccttac tacgttcagc accggctttt
     9781 gattaattct tttgattagg tgtttcgtct gctatttttc cgtcaccgtc gatatcttca
     9841 tccgttaaac gttcgacttc ttttacgaat tctttgctga caacctctaa ttcttcttca
     9901 tctgtacctg tgattgcttc atcaggaatc tgatcggtat tggcaaagaa ctcaatgtgt
     9961 tgatgaagat tttggaaatg catcggtgct tccccgccgt attcaccatc taagttgatc
    10021 attactggtc gattgatatc atcttctgtt tcaacgaaca aactgcttgt tttggtatag
    10081 attattcttg gatcatcgac atgtcgaccg ccattcagca tgagtgctgc aatatgcaag
    10141 atctcaaaga tatttgctgt cttcacaatg attaaagaaa acttcccatc atccaatttg
    10201 gcatctggcg cgatctgttc gaatcctcca acagagttcg tcagccctag gaaaaacatc
    10261 gaagcatttc cgatatattc cccttcatca tacttcaagc gcattttgat tggctttact
    10321 cgagggagca tctctgctcc tttcgcaaga taagcaagat aaccaaaaat actctttagt
    10381 tctgatggaa cttcataagt caattctgtc aaatagccac ctgcagcgat attgatgaag
    10441 taactgttat cagaaaggcc gatgtccatt tttacggttt ggtgtttttt tatcacttct
    10501 gctgctgctt taatattatc tctaggaatt tttaatgctc gagcatagtc gttcgttgta
    10561 ccagctggga tgatggccat tttaggacgg tgttcaagcg gagcgattcc attaaccact
    10621 tcgttgattg tgccatcgcc accagctgcc actactagat caaaacctag ttctgcgact
    10681 ctacatgctt cattcttggc tgaatctggt tctggtgtag ttgcataggc gctggcttca
    10741 aatcctgctt cttctaatgc ctgtaggata tccgccaaat tttttttcaa taattctttt
    10801 cccgaggtgg ggttataaat tacgcgtgct tttttcattt tcagcctcta tcgtttcgct
    10861 aattcatctt taagtagctt attcacgaca ccaggatttg cttgtccttt agttgctttc
    10921 atgatttgac cgactaggaa gccaaccgca cgatcttttc catttttgaa atcatcgaca
    10981 gattgttggt tgttatccaa aatttcatta atgattggca gtagtttgtt cggatcagat
    11041 aattgcaccc aaccattttt ttcaactact tcttgggctt ctccgccatt tgtgatcaat
    11101 tcacggaaga cttttttggc gatttttgag ctgatcgttc catcggcgat caaacgaatc
    11161 attcctgcta agttctcagg tgtcaatttt gtctcagcta attccagttt ctcactgttc
    11221 aagtaagcag aaacttcacc catcaaccag ttagatgctt gcttggcatc tgcaccattt
    11281 tccaaaacag cttcaaaaaa atcagacatt tcttttgtaa gtgtcaatac cattgcgtca
    11341 tactctggaa gtccaaactc acggatatag cgttcgcgac gagaagctgg catctcaggc
    11401 aaactagctt ttactttttg aatccattcg tcatcgatca cgaatttcgg gatatcaggc
    11461 tctgggaagt aacgataatc actagaccct tctttgacac gcatcaaaac tgttttattc
    11521 gttgtttcgt caaaacggcg tgtttcttgt tggatttctc ctcctgaaag aagaactttt
    11581 gcttgacgtt tttcttcaaa agctaaacct tttttcacga aagataaaga gttcaagttt
    11641 ttcaattctg ctttcgtacc gaactcttct tgtccataag ggcgaagaga aatattggca
    11701 tcacagcgca tagagccttc ttccattttc acatcgctga cttctgtaaa ttggataatt
    11761 gatcgcaatg cttctagata agcgtatgct tcttctggtg aacgcatatc agcttcagaa
    11821 acgatttcga tcaaaggcgt gccttgtcgg ttcaaatcaa cgtaagaata accgccaata
    11881 ccatgcatgt tttttccagc atcttcttct aaatggacac gttcgatccg gatttttttc
    11941 tttttacctt ctacttcgat ttcgatccat ccgtcatgac cgattggttg atcaaattgg
    12001 gaaatttgat atgcttttgg attatctggg taaaagtaat ttttccggtc aaaatgagta
    12061 tctttagaga tttcacagtt taatgccaaa gctgctttca tcccgaattc cagcgcagct
    12121 ttattcatta ctggcagcac gcctggataa ccccagtcga tcacgtttgt attgctgttt
    12181 ggttctgcac caaaatgagc tggcgctgga gagaaaattt ttgagttggt ttttaactcg
    12241 acgtggactt ctagtccaat gactgtttca aaattcattc gtctttccct cctaaaatag
    12301 ctggttgttg tttatggaat tctgttgctt gttcaaacgc atatgccgct tgatacatcg
    12361 ttttttcatc aaaataatta ccgatgatct gcaatccaac tggtaatcct tctgataaac
    12421 ctgccggtac agacatacct ggaagtccag ccaagttgac agggatcgtt aagatgtcac
    12481 tcatgtacat tgtgattgga tcgttaatgt tctcaccgat tccgaatgca actgttggtg
    12541 aagatggccc gatgatcaag tcataatctg caaagacttt ttcaaaatct cgtttgatca
    12601 atgtacggac ttgtcctgct tttttgaagt aagcgtcata ataacccgcg cttaaggaga
    12661 atgtacctaa catgatacgg cgttttactt cttcaccgaa tccttctgaa cgtgagttga
    12721 catatacatc atctaatgat ttcacatttt cagagcgata accgtagcgg ataccatcga
    12781 atcgttgcaa attggaactt gcttctgatg aggcaatgat ataataaaca gccacaccgt
    12841 attttgaatg aggcaagctg acttcttcca caacagcacc taatgaacgg aatgtttctg
    12901 ctgctttcaa tacagcttct tttacgccag gggccacacc ttcgcctaag tattctttag
    12961 gtaaagcaat cttcattcct ttgatgtcgc ctgtcagtcc ctctgcaaaa tcaggaactg
    13021 aaacgcctga agaagtaccg tctttttcgt cataaccgct gatagctgtc aatgctaatg
    13081 cattatcttt caccgtacgg gtcattggtc cgatttggtc aagagatgaa gcaaaggcaa
    13141 tcaaaccaaa acgtgagaca cggccataag taggtttcat ccctacgatg ccgttaaaag
    13201 ctgctggttg gcggatactt cccccagtat cactacctaa agaaactgga atttgtccag
    13261 ctgcaactgc tgctgcagaa ccaccagaag agccaccagg tacttttgtt gggtcccaag
    13321 cattttttgt tttcttgaaa taagaagttt ctgtacttcc acccatagca aattcatcca
    13381 tgtttagctt accaacaggg atcatatctg cttgatagat tttatccatg accgttgcat
    13441 cataaattgg ttcaaaattg taaaggatct tagatgcagc ggttgtcaag atatcttttg
    13501 ttacgatatt atccttgata ccaattggaa taccagcaag gacgttgctt tcatcaatcc
    13561 ctttttcatc taaagctttt gcctgttcca atgctttttc ttcgttcaat gtaatgaatg
    13621 cctcgacctc aggctctgtt tctttgattc gttcaaatgt tgtttttgtc aaatcttgta
    13681 cagtgatttc tttcgataca agcatatgat gcagttcttc tagagaacga ttgtgtaatt
    13741 ctgtcattat gcgccagcct ccccattatc gatgattgcg ggtactttga taaagccgtc
    13801 ttctgtttcc ggcacatttt tcaataattc atcgcggttc catccagctt gcgcccgatc
    13861 ttgtcgcatc acgtttactg tttcaaccac attcgacgta aatggaacac cttccgtatc
    13921 tacttcttcc agcatttcaa ccatatcgat gattttacct aattggtcgg taaaacttgc
    13981 aagttcttca tctgcgaacg caagttttgc taatttggca acgtgtttca cttgttcttc
    14041 actaatcgcc atactacccc ttctttctaa acgagttaaa aactttgctc tctattttaa
    14101 cacaagagtg ccgtgtctga aattgtttat tgaaatttgt ctgaaaaaac tcatttttca
    14161 ttcttctact gagcgacttt tagccaatgc atctgccatt tgatcttctg tccaaacttc
    14221 gattcctaat tcttgtgctt tggtcagctt gctgccagca tcttctcctg ctaccacgat
    14281 atccgttttt ttcgatacgc taccagtcac tttcccgcca agattttcga tcgtctcttt
    14341 tgcttcttca cgagtaaatc gtgttagttt accagtaagg actactgttt tttctttaaa
    14401 aggagactcg acttcttgca attgggcatt tcgaagccct ttgtattcga aattgacgcc
    14461 tgcttgtttt aattcattca tcaattcatg gacttcttcg ttactgaaat aagtgactac
    14521 actgtcggca atgatatcac cgatcgtatc aagcgcaata atcgactcat agtcactttt
    14581 actcaatgtt tctagatcac cgaaatgttc tgctaaaatt tttgctgctt tcgctccgac
    14641 atgtcggatt cctaacccga aaatcaaccg ctcgaccgaa ttatctttgc tgttgtcgat
    14701 tgctgtcaaa atattatttg ctgatttttc ttttattttg tccaacgtca ataactcttc
    14761 ttctgtcaat ttgtaaagat cagcaacatc tgctactagt tttttatcat acatctgttc
    14821 tagtacgcgc ggccccaacc catcgatatt catcgcattt cttgaaacaa agtggttcaa
    14881 accttctttc atttgagcag ggcatttagg attgatgcac cgtaaggcta cttcttcatc
    14941 caaatggaca agttcacttc cacaaactgg acaatgtgta ggcaattggt attcttcgct
    15001 gtctttaggt cgtttatcta acacgacttg cgaaacttct gggatgatgt ctcccgcttt
    15061 ataaatcaga actgtatctt tcaagcggat atccttcaac ttaatataat caccgttatg
    15121 caggctggca cggcttactg tcgttcctgc tactcggact ggtgtcatga tagctgttgg
    15181 cgttactacc cctgttcgac cgactgtcca ttcgatgttt tcgattagcg tttcaacttc
    15241 ttctggcgga aacttgtaag ctgctgccca tctcggtgct ttgatagtaa aaccaagttg
    15301 atcttgtagc gagaattcat tcactttgat gacgattcca tcgatttcat agggcaaatc
    15361 cacacgtttg tcatgatatt cttcaatgta tgaccacact tcatcaatcg aatgacatag
    15421 acgtttttcc cgattcgtgt ggaatccaat tttttctagc tcttctagcg catcatactg
    15481 ggtttttgct tgcataggac cgaaatcagc tactgtgtat aaaaaagtgt ccagatttct
    15541 tttcgctgtg attttagaat ctaactgacg caaagaacca gctgcggcgt tccgaggatt
    15601 cgcaaagata tcttttccct ctgcttctct gtcttggttc aattggacaa atgaccgttt
    15661 tggcatgaaa cattcgcctc ttacttcgat gttcattggt tcttttaatt tgattgggac
    15721 cgaccggaca gtttttaggt tttcagtgat gttttctcct acagttccat ccccgcgagt
    15781 cgcacctctc acgaaatttc catcttcata acgcaaagaa acggaaagtc catctatttt
    15841 tagttcacag caatagtcaa ctacacgtcc cactgctttt tgtacacgtt gatcaaaagc
    15901 tatcagctct tctttgctga aaacgtcgtt caaactgtaa agcggaatat catgagtgac
    15961 tttttcaaaa ccttctaata cttttccgcc tactcgttga gtgggagaat cagacgtgat
    16021 caaatcagga tattgttcct cgattgctac taattctgcg tattctttat cataaacata
    16081 gtcttccact gttggtttat cttctacgta gtattcgcga gaccattggt tcagtcttgt
    16141 acgaagttct tctgctcgtt tagcagcttc gtcaatcgtc atttcatgtt ccatcattta
    16201 ccctccacta ctaagctttt tcaattggcg caaaggcagc caaaagacgt tttactcctt
    16261 gacttgggaa agccacatct agttctagat ccttcgacgt cccgcttact cggacgactg
    16321 ttcctagacc ccattttttg tgtttgactt tatctccagg tttccagctg ttattttccc
    16381 ctccagtagc acttttagag ggaacaaccg ctgtctctgg ctgtttatag ctatatgctg
    16441 tttttttagc gaatacaggc gtttttttag gcgtaggacg catgccttcg atttccagta
    16501 actcctgatc gatttcttct acaaaacgag aaggtcggtt atattgcgtc cgtccatata
    16561 atgttctgga gaaagcattt gttaaataca acgcttcttc tgctcgcgtg atccccacat
    16621 atgcgagacg gcgttcttcc tccagttcgc tttcttccat caatgctcga gataaaggaa
    16681 aaaccccctc ttctagacca atcaggaata caactggaaa ttctaatcct ttggctgcat
    16741 gcaaagtcat caatgtcact tgggaagcat cttcttctaa attgtcaatg tcggaaacaa
    16801 gtgccagatc atttaagaag accgttagtt tttcttctgg tgcatcagcg tcttcttcat
    16861 tttgctgttc gaattgtttg tcgaattctt gtgtgaccgt taaaaattcc tccaagtttt
    16921 cgatgcgtgc ttgtgcttct aatgtatttt ggatttttaa gtcttctaga tatccgctac
    16981 gatccaaaac ctctttcgtc aattctgtca ctgtcaagta tgggatcatc tgagtgactt
    17041 cttggatcat ctctccaaat gcacctaatt gctgtcctgc ttttccaccg atatttgcca
    17101 gatccacatt ttgcgctgct tcaagcagtg gccattcatg aagagaagca aaactgcgta
    17161 gtttttcgat agatcctggt ccgatgcctc gtttgggcga attaacgata cgctcaaaac
    17221 tgattgaatc ttgaggattt accagcacat tcaaatacgc taaaatatct ttgatttctt
    17281 tacgatcgta gaacttgtgc cctccaacca ttttataagg aatatttgct ttcaacagag
    17341 tttcttccat tacacgagat tgtgcgttcg tccggtacaa gatggcaaag tcgccgtaat
    17401 tccgatgatt gcttcgaatc tcttcttgca tccggtctac gataaaacgg gtttcgtctc
    17461 gttcattatc tgcacggtag taagtgatct tgtttccttc tttgttttct gtccacaagt
    17521 tttttggttt tcgattgcta ttgttttcaa tgacttggtt tgctgcgcta aggatgtttt
    17581 tcgtggagcg atagttttgt tctagcaaaa ttactgcagc atctggataa tctttttcaa
    17641 aatccaagat gttctgcata tctgcaccgc gccagccgta aatactttga tctgcatctc
    17701 ccacgacgca caggttccta aatctacctg ccaataaatt gactagcgta tactgtgcgt
    17761 gatttgtatc ttggtactca tctacatgaa tataatggaa tttgttttga taatacgtga
    17821 gtgaatcagg atgttcttca aataatcgaa tcgtattcat gatcaggtcg tcaaagtcca
    17881 tacattgatt atattgaagc tctttttggt aagcagcata acattttgcc gcaatctctt
    17941 cgtaataact gccttgcatt tttgcaaaat cctgaggggt ctgcaggctg ttttttgctt
    18001 gagagattgt tcctaatata cttcgaggat catatttttt cggatcaatg ttcagttctt
    18061 ttagtatccg tttcatcaag gtcagctgtt cagaagaatc gatgattgta aagttccggt
    18121 tatacccgat aaaatcaaca tctcttctta aaatacgcac acacatcgag tgaaaagtcg
    18181 atacccagac atcttcgcct ccagatgcga gaatcgcatt gacacgttct ttcatttctt
    18241 ttgcagcctt attggtaaaa gtaatcgcta ggatgttcca aggattgact tctttttcct
    18301 caatcaaata ggcaatgcgg tgtgtcagca ctctcgtctt tccacttcct gcacctgcca
    18361 ttagcagtaa tggtccgtca gtgtgaagaa cagcttcttt ctgacggggg ttcatcccgt
    18421 ttaataattc agctttggat gacattaaca acatcctttc ttttatcaat cttgttaatt
    18481 atagcatttt cttggctttc attaaactag ctagttcaaa atcatttgtt tctttttatg
    18541 gatttttcct tgtcaattcg ttgatttcgc gctaaattaa aagaaagcga ggcgtttgaa
    18601 tggaaaaaaa aggaaaaatc aagaaattag ataagactca gatatatttt attctaaaag
    18661 gaatatttgt tggactgctg acaggaatcg tcgttagcct gtttcgatta tctatcgaaa
    18721 agctaagcga tttagttcga agtatctatg aaatgagtcg ggaacagccg atttacttaa
    18781 ttggcctagc tatttgctgc atccttgcag catttttcgt gggttatctg gtcaaaaatg
    18841 aaccggatat caagggaagc gggattccac aagtagaggg acagcttcgc ggggaattat
    18901 caatgaactg gttctctgtt ttatggaaaa agttcattgg cggtgtactt tcagttggtg
    18961 cgggcctttt tctaggtaga gaaggacctt ctatacagct tggcgcatcc attggacaag
    19021 gagctggaca attgtttcgg agtccatctt cagaagaaaa gattttaatt tctagcggtg
    19081 caagtagcgg acttgcagca gcattcaatg ctcctatcgc aggattactc tttgttttag
    19141 aagaaatcca tcactccttt tctccacttg tttggctgac atcatttgct tcagcaatca
    19201 ctgctaactt cgtctctctt tactttttcg gcttgagacc tgttttatat ctaggtgacc
    19261 ttccttcttt gccgttgaaa tattatggtt ccttagtgat cttaggagta cttctaggca
    19321 tcttaggttt tatttatcag aatgtcttgc tggctttgcc aaaattttac aaccgattgc
    19381 ctttaccagc ttattttcac gggttcgttc ctttcttgct gattttgcca attggttatt
    19441 tatggcctag tactctaggc ggaggcaatc aaatcgtttt agcttttgga catgcttcct
    19501 tgccaatcat tatgattatc ggtttgtttg ttcttcgatt cgttttttcc atgatttctt
    19561 acggtggggg tcttccaggg gggattttcc ttccgatatt gacattgggg gctttaatcg
    19621 gtagctttta tggaaacatt atggttgatt tcgggatgga tccaatctac atcaaggatt
    19681 ttgtcgttat tgccatggct ggttatttca cagcgattgg aaaagcaccg ctgacagcaa
    19741 ttattttagt gacagaaatg gttggctctc ttaatcatct gatgccttta ggattggctg
    19801 ctcttgttgc ttatatcgtc aatgaccttc tcggcggtaa tccaatttac gaatcattat
    19861 tggaacgtct acttggaaat actcagaccg cctcactaaa aggtgcaagg acaacttttg
    19921 attttccagt aacggcagag agttctttag acggattaat ggtacgagac ttcaattggc
    19981 ctaaaaatat gctgctaatc tctatacgca gaggaaatca ggaaatcttg actcacggtg
    20041 atacgatcat gaatgtagga gatattttgg ttatcttgac tgatgaagca aacttgccaa
    20101 gaatcaagca ggaaatcgag gctaaatctt ctatcacaaa tttgaagcaa tcattttaac
    20161 gaacatggaa aaactatttt acaacaacta ttcatctaca ctacttgatt ctatatgatt
    20221 ctataaaaaa agaagaggct gggaaaaccc agcctcttct tttttatttc tatgcttctg
    20281 ctactgcagg agcttttctc aataaaccat aaatcacacc agatacgact gctccgatag
    20341 caataaacac caaatacatg accgggtgac tcagtaagaa tacaacgaaa atcccgccgt
    20401 gaggagccag taatttgatt ccgaaagctc cgactaatcc gccagttaaa gcagaaccaa
    20461 taataaaact tggaatggca cgaatcgggt cagctgctgc aaatggaatc gatccttccg
    20521 tgacaaacga tagtcccatc acgatgttcg tcaaaccagc atcttgatct gttttgctga
    20581 atttattctt aaacaaacgt gtagcaacga aaattgccaa tggaggtacc attcctccag
    20641 ccataacaga agccatcacg acacttccgc cagttgcaac agtcgtagct aaagttgctg
    20701 taccaaacac ataagcggct ttgttgattg gaccgcctaa atctgccgcc atcattccag
    20761 ctaataatgc accaagtaaa gcagcattcg aaccactcat acttgtcaag aaaccgttca
    20821 atgcatcatt gattgctttc atagggacat tgaccagcaa catcaagaaa ccagtaatca
    20881 gtaagccaaa cactggataa aagaggatcg ttttaattcc atccaatgat tttggtaagt
    20941 tcttgaataa tttacgtaaa aatacgatga cataaccagc taagaatcca ccaatcagtg
    21001 cacctaagaa tcctgcaccg cctgtattag ctaatgcacc agcagcaaat ccaacgatca
    21061 aaccagggcg gtcaccgata cttgaagcaa taaatcctgc taataccggc agcatgaaac
    21121 caaatgcagc ttgtccgatt tggttgaacc agctagcagc ttgattgtaa ttccctagat
    21181 ttgataattg atcatgtggc acgccaataa attggtcgat catgaaagaa agcgcaatag
    21241 cgattcctcc accaataacg aaaggtaaca tgtgtgatac gccgttcatc aaatctttgt
    21301 aaattctttg tccgatagta ccatccgcat ttccttcttc tgaactgctt tcttttccat
    21361 caccatggaa tactggtgca gtaccactaa gggctagatt gatcaactct tctggtttac
    21421 ggataccatc actgacagga cgattgatca gttcttttcc gtcaaagcga ttcatctcta
    21481 cttttttgtc tgctgcaacg attacacctt ctgcacgggc gatgtcttca gcagtcaatc
    21541 ggtttttgat tccttctgaa ccgttcgttt cgactttgat ctccacaccc atttctttcg
    21601 cttttttctt caaagcatct tctgccatgt aagtgtgagc gatccctgtt ggacaagctg
    21661 taacggcaac aacgaatttt ctttctggat gagcagaagc ttctgcagct tgtgtttctt
    21721 gttctgcttt ttcttctgct tctttttgct cttcagctgt ttggaacaag tcttggactt
    21781 cttctggtgt atttgcttct tttaattttc ctacgaaatc tgcatcaatc agcaatcgag
    21841 atagtgctgc taatgcttgc aaatgtgtgt cgtttgcgcc ttctggagct gcgatcatga
    21901 aaaacagata agttggctgt ccgtctaagg cttcataatc tactcctttt tggctttttg
    21961 cgaataaaac tgttgcttct ttaactgcac tgtttttcgc gtgaggcatg gcgattccat
    22021 caccaagacc tgtagaagtt tgtgcttcgc gggctagaat tccttcttta tatgtttcga
    22081 tatcggaaat ccggccggca tcaaacattt tttggaccat ttcatcgatt gcgccttttt
    22141 tatcagtcgc ttgcaaatcc atgatcatcg cttctttgac cattaaatcc ttgatattca
    22201 tgattatcct ccttgaaact aatcaatttt tgtgatttca acttctggca gcaactcatc
    22261 aatcaattcg cgagctgcca agtcatcaga aaaagctgtt gcgcttccgc aagcaactcc
    22321 ccatttaaag gcttcgattg gatctttcgt acggctgtaa gccccaataa agccagcaat
    22381 cattgaatcc cctgcaccta ctgaattttt caatgggcga actaatacat tcgttttata
    22441 agttccttgt tccgtgaaga gcaaagcacc gtctccagcc attgagatca gcacattctg
    22501 tgccccttca gctagtaatt ttttcccata cggaatgatt tcttctacag attggaaggt
    22561 tgtgttgtaa agctcagcta attcgtgatt gtttggtttg atcaacaacg gtttcttaac
    22621 aagtgcattt tttaggtcat cacctgttgt atcaatcacg aaatctgcac ctttttctcg
    22681 aatgatatca atcagttctt gataaaatcc ttttcgcagg ctagctggcg cgcttcctga
    22741 caatacgacg atatctcctt gtcctactgt tcctaatgct tgcttcaact catcgatttc
    22801 ttcatctgaa atagatgggc ctaagccatt gatctcagtt tcagtgtctg attttagttt
    22861 gatattgatt cttgtatctt cttttacggt tgtgaacaaa gactggattt gttctttttc
    22921 aagccacttc gaaataaatt cgccagtgaa ccctcctaaa aaccctagtg ctgtagattg
    22981 agaatcgatc cgtttcaaaa tacgggaaac attgatccct tttcctccag gtaacttgaa
    23041 gtcttccttc attcggttca aatcgcctag ttttaaccca tctacgcgta cgatataatc
    23101 aattgatgga ttaagtgtta ctgtgtaaat cattgtattg cctccttaat tgttgtcttt
    23161 tcacggaatt cattaacgaa ttctaatgga caacgctcgg ttatgatcgt ggctgcttcc
    23221 aaaggtgcaa cagaggtgaa ggacaactgg ttgaatttgg tatgatcaat caatacaaat
    23281 gtctcttcgg aatttttcat ggctaaacgt ttaacagctg cctcttctgg atccggagtc
    23341 gtgaatcctt gttcaagatg ggcaccgttc attcccataa atgctttatt aaaatgaaaa
    23401 ttttttagtt gttctatcgc attagatcct aaaacagcat tagttgccag ctttatcgtt
    23461 ccacctaaaa taatggtagc aagctccaaa tcaactaaat ttgccgcatg ttgaacggaa
    23521 tttgtcacta ctgtgatctg ttttccttgc agaaaaggaa tcatttctaa cgtagtagag
    23581 ccagcgtcta aataaatgac atcttcgtca ttaatggttt cagctgccga tttggcgatt
    23641 tgctgttttt cgtgaacgtt tttgagtgat ttttcactca tattctgctc aaaattcaat
    23701 aattgctctt tcttagctcc gccatgaact ctttctagaa acccactatt ctccagctct
    23761 tgtaaatcac gtcttatcgt tgattcagaa gcattcaaaa ggtttacaag atcttgtgat
    23821 ttcacgattt tctgctgttc aagcaagtct aggatttttt gtctgcgttc ctctgtaagc
    23881 attttcacga tcacctcaat acaaataata gcatattttt tttctactag caatataaat
    23941 cagtcaaaaa cgttcataaa aattataaat cagtcaaaaa aaaataaaaa accagtcaga
    24001 aagactggtt ttttattttt ttgtctatca tcaagccgtt ttatcgtttt ttattttcgc
    24061 ttcttgtttt tttcatataa acagaagcag gttttctttc cttatgaaga agataataat
    24121 aaagtcccat cccgccaaac atgaccaaaa aaccgatgac tgatcgaata ggaaaattag
    24181 gaacaaaaaa gagacagacg gataataatg atgccggaac cattattcga taatagagtt
    24241 gtttatccat tttcttcacc tcatattttt tgtaaagtaa tcaattgttc taattctcgg
    24301 taatctaaaa attgcacacg cagatgctgc tcacctttct tgacatcgaa ttggatactt
    24361 cgccgctttt tctttattcg tttgaaggta acatcagaga gtctatgttt gatttccttt
    24421 aacggtaatt ctgacaggga ttctgaagga acatagattc ttaaggataa aagatactct
    24481 tgatattttt catctgttac ctctacttct agatattccc taatataata acaataagct
    24541 tcgattccat tatccaaagc aacagtcgga tcgataactt tatagcacat catgcgtgat
    24601 cgattctcgt caacccaagg aaaatacgcc tgagcattta gtgttccgcg aacacgcctt
    24661 tctttataat ctccttgttg gatatcataa ctttttgtta caaaaaatga agcattcttt
    24721 ttaggaacag gttgcatttg tgcattctga tactggacag attggatcgt atggtcagtg
    24781 taaatgtatt caacgaatgg cggtttcaat aaaccgacac cattgtccca agcaagatga
    24841 tcaaacccaa gctggtccct aaaataggat tgaaacattt gattgtttgg cttttgccag
    24901 ttaaaggggt ctcgataaag ctggatacat atgacttcgt tttctaattc cttcactttt
    24961 tctacttgtt ggatgtcgag aaaaatcggt ttagggatga tttgatgata atagcgacta
    25021 taatacattt cccaacacga agtgaaacat aaaccttggt aaaaaagatc gtatcctgga
    25081 aattggttgc aatcgactac tacttccccg ttttgttgtt tcattttagg taaatgttcg
    25141 atttcatcct ttttctcaaa caatagccgt tgagagatct gttttgtatt gtgatagata
    25201 aactcgctat aagctcgcat ataggcgaac accccatgag caatcatttt tttctgaatg
    25261 aagtctcgaa tcaaaaattg cttttctttg aaaacagaga tagacaatac attttttatc
    25321 aataattgat tatcttccaa gcgaaaacta cattcgttct cgtcatttcc tacaactaac
    25381 caactttcgt ctcttaccga tgctagcatt attttttctt gaagctttcg aagatcatac
    25441 ggatccgctt gttgtgtgaa agagcggata aatgttggct ggatcaacag acgtgtcaaa
    25501 atagcccaat cgcttatttc ctgttttttt tcttcttcat ttaagtctgt gagctggata
    25561 gtcatcaata atttatcttc gccaatcatt ttctcacctc tttcaaatct ttttataatt
    25621 taattataac caatgataaa gaaaaactaa aactttatgt cttagaaacc aatttggacg
    25681 attacatgat agactaagta aaaagaaaca aagaggtgtt tacaatgggg ttgaaatttg
    25741 aacgtacaga taatttagat aaacttttcg aatcatttgc tgtcgatccg gaaaaaacag
    25801 ttgcaccaaa agaagagatc gatcgatttc taaaaccaca gccacaaaat gaaaagaaaa
    25861 aagacaagaa acaaaagtaa gttaagctca aaaaaagact taccagccaa acttttatga
    25921 aagagtttga ctggtaagtc ttttttattc tttttatgat tcaattgctt gttcgattgc
    25981 tgcccacgct tcttcttttc cctgttttgt aacggaagaa aagagaataa acgtgtcatt
    26041 gcgatcaaaa tccaacgctt ttttgatttc gctctcatgc ttgttccatt taccacgagg
    26101 gatcttatct gccttagtgg caacaacaat tacagggata tcataatact tcaaaaattg
    26161 atacatctgg atatcttctt gagaaggctt atgacgcaaa tctactaaag aaacgaccgc
    26221 acgtagctgg tctctagtcg ttaaatatgt ctcgatcatc tttccccatt ttgctcgctc
    26281 agttttggat acttttgcat acccatatcc cggaacatcg acaaaatgta atgcgttctc
    26341 gatcaaataa aaattcaatg tctgggtctt tcctggctta ccggaagtac gggctaaatt
    26401 ctttcgatca atcaatgtgt tgataaaaga agatttccct acatttgatc gtcctgccag
    26461 agctatttct ggcaattgtg tatctggata ttgttttggt gccactgcac ttatgataat
    26521 ttcagcttga tggactttca tggtctctct ccttaacaag gtgatgaata aagcaagcgg
    26581 ctgtcgcaaa agccaaaatg acttttacgg cagcctcttt tcatcaaccc gcttttttct
    26641 ctgaatcttt atagattact gttggctcac cagacaattc agctgcttct tttgtgataa
    26701 tcactttttc gatgttttca tttgaaggga tatcaaacat cacatccatc atgatttctt
    26761 caatgatgga acgaagtcct cgagcaccgg tattgcgttc gattgctttt ttcgcaatgg
    26821 ctctcaacgc ttcaggttcg aattcaagtt cggtattatc tagagaaaga agtttttgat
    26881 attgtttgac tagcgcattt ttcggctcag tcaagatccg taccagatca tctgttgtca
    26941 atttctctaa agctgccata acaggtaatc gtccgataaa ctcagggatc aatccgaatt
    27001 ttagtaaatc ttcaggaatg atatgctgca tgacactttc gtcttcagaa agtttttggt
    27061 tttgtgtacc aaatccaatc gtcttttcgc ccatacgatt cttcacaatc gtttcgatgc
    27121 catcaaatgc accacctacg atgaacaata tgttcgtcgt atcaatctga ataaattctt
    27181 gatgtggatg tttacgtccg ccctgaggtg gcacacttgc aacagttcct tctagaattt
    27241 tcagcaaggc ttgttggaca ccttcgccgg aaacgtcacg agtaatagat acattttcgc
    27301 ttttacgcgc aattttatcg atctcatcaa tatagatgat ccctttttct gcaagttcca
    27361 cattgtaatc agcagattga agcaatttaa gcaaaatgtt ttctacatct tcccccacat
    27421 aaccagcttc tgtcaaactt gtcgcatctg cgattgcaaa aggtacattc aatgtttttg
    27481 ctaatgtttg tgctaaaaat gttttaccag accctgtagg tccgatcaag cagatattgc
    27541 ttttttgcag ttcaacatca tctgtgtttt cttgtgcttg ctgattgaca cgtttatagt
    27601 gattgtatac ggcaacagct aacgagcgtt ttgcgcgatc ttgtccaatc acgtagttat
    27661 taaatacttc aagaatttct agtggtttag ggacttcagt gaattcgcgt acagcttctt
    27721 cataaaattc ttcatcaatg atttctttac ataggtcgat acattcatta cagatgtaga
    27781 cccctggacc agctacgatc tttttcactt cttcttgggt tttcccgcaa aaagaacagc
    27841 gtacggtctc attgctactt ggattgtcat acatggtctt caccccttaa cttgcaaaaa
    27901 gtatgcaaga aactatcaaa gactttacag tcttttacta tcataacata gtttagtaaa
    27961 aagaaaaagt gtttaacaaa ggatcaactg acctatacct ttgcattttt tactcttaac
    28021 aaaaaataag gaactgtgac aaaatcgtgt cacagtccct tatttcgaat aaacaatttt
    28081 ctagaagtag cttcttcaga aatgatttat tacttgattt ctattctttc gcttctgtcg
    28141 ctgtacctgt gatcaattcg attgcttttt tcattgtcac atcatgttcc agcatatctt
    28201 ctgtcaacac gcgtttgatt tgttcgattg gcatgttgta tgtttctgat aattcttgga
    28261 tttctgcatt gatttcttct tctgttgctt gaagattttc agcagctgca attgcttcga
    28321 tcacaagatt tgttcttgtg cgcatttctg cttcaccttc gaattgttta tgcaagtctt
    28381 cttctgtaga accagttaat tgatagtaca tttcaggaga gataccttga cgttgcatgt
    28441 tgttcaagaa ttcgtccatt gaacgatgta cttcatcatg aaccatcacg tgtggcaatt
    28501 caacgatttc tgcgttttca acagccatac ggatcgctgc ttcatctttt gcatcgtccg
    28561 ctgctttttc ttttgtttct actaattctt tgcgatattt ttctttcaat tcgtctaatg
    28621 ttgatacttc gtcatcaaca tcttttgcga attcgtcatc taattcaggc acttcttttg
    28681 cttttacttc atggattgtc actttgaaga ctgcttcttt tccagcaaga tcttctgctt
    28741 gataatcttc tgggaaagtt acatttacat ctagactttc acctgattta tgaccaacta
    28801 attgatcttc aaaacctgga atgaatgagc ctgaacctaa ttcaagtgag taattttcgc
    28861 cttttccgcc atcaaacgct tcgccatcta caaagccttc gaagtcgatt actactgtat
    28921 cgccattttc agctgcagca tcttctttga taactaattc agcttgtgct tcttgttcac
    28981 gtttgatacg tgcatccacg tcagcatctg ttacttcacg gtcttgtttt tcaacaacta
    29041 gatctttata ttgtcctaat tttacttctg gtttcactgt tacttcagca gtgattaccc
    29101 agtcttgacc tttttccatg ctttcaacat cgatttttgg ttgagaaaca ggatcgatgc
    29161 cagcttcttt gacagcagct tcataagctt ctggtaatac agcattcaat gcatcttcat
    29221 ataaagcttc ttcaccgtac atacggttga atacttgacg agaaactttc cctttacgga
    29281 atccaggtac gttcaagctt tttttcactt tattaaacgc agttgttaat cctttttgga
    29341 tttcgtcttg cgcaatcgta aattttaaaa caccatcatt cgtgcctttt ttttcaaatg
    29401 ttgcagtcat ttaattccct ccgagtttag aaattcattt tatacatcaa aaacgtttca
    29461 tgcatacctt taaatagtac actaaccctt tcaaattgta aacatattga atggatagaa
    29521 tccttgtttt tcataacatt tttatctttt tcactgataa aatctaatgg aagaagatca
    29581 tttgctgcaa aattttttct ctttttttta aaaaatcttg tttttcatct tctgtacctt
    29641 catacatcgt ttttcctgta cacgcctcta cagtcagttg aaaccagcgc ttcgggtctt
    29701 cgatgtatgt atcatagaca ggataaacaa ttgcagattg caacgtgaat tcttctttta
    29761 acacggaact aagcagcgca tcttcattcc cgtaacgatc tgaaaataca cataattgct
    29821 ggtaacttgc ctgctgctgc ggctttggca gatcttttgg tatgactttg actaactttt
    29881 cttcaatcgt caaatagaaa acaggatggc attcgttcaa ttgcctaagt gtctccagta
    29941 attttgccct tgctaaaata tggacctctg gcgatacaat aagtttactc gctgtttgga
    30001 taaactcatg ataaggaagc tgttcgatct catcaataga cgcaagctga cgtgcaggtt
    30061 ccaacgaagg aagttcctcc gatttattgt ggagtgcctt gatcttctgc ctctccattt
    30121 gtccggataa ttcttctgca aattggattt tttcaatgac ccgctgctcg aaagcttcat
    30181 aaaaatctgt acttgctatc aattttctag catataaaaa atcttgtgta ctgattaaca
    30241 agtcaaaata aaattcagca aactcctcat tttgaagata ctgtttttca tgcaagagtg
    30301 cttgcttcaa tgcttctttt ttctcgtcca actcgtaaag acaaaaaaca agcaggctat
    30361 tagcctcaaa gctttctttc aactcgtaag ctcgttgaaa taattctttt gcctgctgat
    30421 attgctggtt tgctagtgct tgttgtgcca ctcgcattaa attctcatat ttttctggaa
    30481 aatcaatcgg cttcacgcag tgtcctcctc ttgctcatat tcagctcatg ttcaatagta
    30541 gcaaaacaaa aaaagcatga caattaaaaa taactgaagc accctctaaa agctagattt
    30601 tagatctgac ttttagaagg tgctttcaac tgttactgct ttttattgct catcagctgt
    30661 tgacttcctg gttgaacgct ttgatcagat tgatcgtaac aacacttgta ttagtgaaaa
    30721 aatcgcccag atgttgcttt ttctgattaa agactgctgg aagataccct aacatcagca
    30781 aaggaaattt tagaatgaac cggcaagcac cttctctaac taaaacggtt gaccagctaa
    30841 gttcttcttc atcaaagctg atgactcgaa taccaaagat cattttccca atcgtttggc
    30901 catgattgaa tttcgtcaat aaaataaaat acgctagata aataagtaag gctgtgaatc
    30961 cgtagagact cagataagaa tctgaaaaat taagccaacc tagatttttg gcaataccaa
    31021 gtgttaaacg cgtaatagct ccgatacaca aaagatcgac caagaaggca aacattcgga
    31081 taaaaaaacc tgcatagaaa tacttaggaa aatcatttgc ttttggcggt tcatttcttg
    31141 tgtccgtata ctttttccat ttacgttgtt tctttttgat ttcttcgggc gtcaaaggct
    31201 ctctttcaaa agagctcagt accttttggg aaaagttgga gtcaggtcct acagcaggct
    31261 gctgttgctt atctttttct tgttccttat tttcatttga aagctctgac atgcttactc
    31321 acctccgtaa taatacattg cttttggtga ttccgctgtc cctagattct caagaacgga
    31381 taagatctga ctagtctcag aaggcttcaa tccctggaat tctgctagtt tacttcctaa
    31441 ccatgtacta gcaaacccag atggcgtgga agaatattct actagttccg catcttcaag
    31501 ctgttgttcc tttcgtatag cttttaaagc atcttcagga aatcccaatt catcgactaa
    31561 acctacttct tttgcttgga caccatcata aatccttcca tcagcaattt ttttgacttc
    31621 ttcttcagat ttattccgtc cctcgctgac gattgctaca aaacgactat atgcgctgtc
    31681 gatatatgct tgaagcaccg cttcttcttc ttttgtctct ggtcgcgttc ctgaacccat
    31741 atctttcaac gctccgcttt taactgttgt atcttccaca cccagttttt ctagaagtcc
    31801 tgaataattc aatcctgaca tgatcacacc aattgagccg gtcactgttt cttctgttgc
    31861 aaagatttta tctgcttgag cagaaatata gtagccaccg ctagctgcca tgtttttcat
    31921 gctgacatac attgggatat catgttcttt acgaatcgta tccaatttct tagcgatttc
    31981 cgcactttcg tagacaccgc caccaggaga atttacttct aaaaagatac ctttgactgt
    32041 tggatcttct tcaatcgcct tcagttgttc cataaaattt ttatgattgt aaccttcaga
    32101 agcaaataaa cctgaagacc ctcccgcagc aatcgttcca tctactgata attttacgat
    32161 tttttgcgta ctgtctccct cttccaacac atttggagct agttcttcat ccccatataa
    32221 ccaactgttc aatccctcaa tttgatcgtt ttcctgaggc ttagtcaaac tagctgaaac
    32281 taacgaaacc acaaataatc ctgcagcaat cagaactgct gcccaacgtc ttttattcat
    32341 tctttctcct ttctaataca agttttttat gacgatcaca gtttctgact gctcagaagt
    32401 gtcgaaccgc tctgcttttt cttcccgcgc atacccggaa aacgactctt ttgttaattc
    32461 atcttctacc gtaaactcac gagcttgagc aacttccaag gcatcaggtg cgatttcttg
    32521 gatgctatcc cagccagtat ctaaaaatat tttcgtatca tctgatacac cagctgatgc
    32581 ttcgaatttg gacaattgtt tttttaacat tcccagtgtc atgatctcaa tttttgaatc
    32641 catttttact cctcctctat ccgattactt tctattgtac cgaactcctg aaaaagatga
    32701 tagataaaaa cccattattt tttgacataa aaaggacatt tgacaaagaa tttgatatat
    32761 accaatttat tttttattct ttttttgttg acaagaacat tcgcaaaagg ttatgatttt
    32821 aaatgtttcc agataaaaaa caaaaaggag gatagttata ccttttagga tagcgccagg
    32881 tctgtgtaaa cagatgacga ggagagggtt tatcgaaaga ttcggcggat ggccctaggg
    32941 actgcactct acagaaaatc cacaaaaaat gtctgcaaag ataaaacaaa aggattttcc
    33001 tgcagctgga agcattcata cgaattcatt atagaaagta ggatgttttt tatgtgcgga
    33061 attgttggaa tgattggatt aaaaaatgta acaccaggat tgattgacgg tctagaaaaa
    33121 ttagaatacc gtggttatga ttcagctggt atctttgtct cagatggcac aactgattat
    33181 ctggtcaaag cacaaggcag aattcaaaat ttgaaaaaca aaataaatat tgatactaca
    33241 ggaaatatag gtattggtca tacacgatgg gcaactcacg ggcaaccttc tgaagaaaac
    33301 gcccatccac atacttccca aagtggacgc ttcgtgctcg ttcacaacgg cgtaatcgaa
    33361 aactttgaag aacttaaaat tgcttattta gcaaatgacc actttattgg tgagaccgat
    33421 actgagatta tcgctcatct aattgaaact ttcgcgaagg atactacaac gcaagaggcg
    33481 tttttaaaag ctttgcgggt catcaaaggt tcttacgctt tcgctttgat tgatcggaca
    33541 gcacctgatg ttatttacgt cgctaaaaat aaaagtccac tattaatcgg actcggcgat
    33601 ggattcaatg tgattgccag cgatgcaatg gctatgcttg ctcatacgaa ggaattcgtt
    33661 gagattgaag atgaagagat ggttaccgtc acatcagaaa aagttattat tcaaaatttt
    33721 gcaggtgaca tcgtagaacg ttcttcattc gaagcacaag tagatgcttc agatatcgag
    33781 aaaggtacct atccttttta tatgctgaaa gaaatcgacg aacagcctat tatcatgcgt
    33841 cgtatcgctc aaaaatacgt aacagaagac aatcgtgtct ctcttgatca aaagctggtc
    33901 gatacattgt ctgatagcga tcgaatctat atcgttgctt gcggaactag ttaccatgcg
    33961 gggttagcag gtaaacagac tttggaaaaa ttgacacaga ttcctgttga ggttcattta
    34021 gccagtgaat ttggctataa cacacctctt ctttcaaaaa aaccattctt tattttcttg
    34081 agtcaaagtg gcgaaacagc agacagccgc caagtgctgg taaaaatcaa ccgtctcggc
    34141 tacccttcac ttacgattac aaatgttgct ggttctactc tttcgcggga agcatccttt
    34201 acactgctat tgcatgctgg acccgaaatc gcagtagcat caacgaaagc ttatactgca
    34261 caaatagctg tactaacttt attagccaaa gctatcggtg ataaaaaaga atttgaaact
    34321 tctcttgtat tcgatatcac tcatgaattg agtttagttg ctaatgcgat ggaatcggtc
    34381 atcgcccaaa aagattattt agaagagttg gcagaagtat atctcagcga tacaagaaat
    34441 gccttttaca ttggacgtgg tgctgactac aacgtttctc ttgaagctgc tttgaaattg
    34501 aaagaaatca gttatatcca ggcagaaggt tttgccgctg gcgaattgaa acatggaacg
    34561 attgctttga tcgaagaagg aacaccagtg atcggaatca tcagtgaaga agtgactggt
    34621 gcgcatactc gaggaaacct gaaagaagtc gaaagccgtg gcgctaaaac actagttatt
    34681 gtctcagagg gattagaaaa ggaaagtgac cagctggtgc taccaactgt ccacccttat
    34741 ctaactactc taaccacagt cgtaccgact caattacttg catattacgc aacgctatta
    34801 cggggattcg acgttgacaa accacgtaat ttagcgaaat cagtgacagt agaataataa
    34861 aagaacaagg accagtgtca gacatctgac actggtcctt gttcttttat tttttatgaa
    34921 aatatctctt aatccagtcc gatttcttct ttgactactg ccgcgatttt atcgacatag
    34981 taattaactt tctcatcagt aggcgcttct gccatcactc gaagtaatgg ttctgtaccg
    35041 gaaggacgaa ctaggattct tccttctgat cccatttcag cttctgcctc ttcaatcact
    35101 tttttgattg ccggaacatc catcgcaccg tttttatcac ttacgcggat attcactaat
    35161 ttttgcggat agattgttac ctctgctgca agctctgata atttcttacc tgtctgtttc
    35221 atgatattca gtaactgaat tcctgaaagc atcccatctc cagttgtgtt aaaatctaag
    35281 aaaatcatgt gcccagactg ctcgccgcca aagttataat cgtttttgcg catttcttct
    35341 acaacgtaac gatcaccaac ttgtgtaacg acatctttca aaccgattcc ttccaccgct
    35401 ttgtggaatc ctaagttact catgacagtc gtaacgatcg tatctttttt caatcgattt
    35461 ttttctgcca agtatttagc acagataaac atgattttat caccgtcgac aatgtttcca
    35521 agttcatcca ccgcaataat acgatcgcca tcgccatcga aagctagtcc agcatctgct
    35581 cctttttcaa cgaccatctc agctaaacgt tctggatggg tagagcctac gccgtcattg
    35641 atatttaacc cgtctggatt tgtgcccatt gtataaaaat ctgtttcaag atcggcaaac
    35701 aaacgattaa cacttgttga agttgctccg tttgctgcat cgacacaaat ggtcaaaccg
    35761 gaaaggtcac cgttgatcgt ttgtacaagg aattgagaat atttcaataa gccttctgga
    35821 aattcttcta cagttcccaa tccttcagct gatggacgag gcaattcatc cacttcagca
    35881 tctaataatg cttcaatttc tagctcttgg tcatctacta gtttaaaacc atctgcaccg
    35941 aaaaatttga ttccattatc ttgtgctgga ttatgagaag cagaaatcat gacccctgcg
    36001 cttgcttttt gcaaacgtgt cagataagca acacctggtg tagagatcac acctagctgg
    36061 aacacttcga tacccaccga taaaagtcct gaaatcagcg cttcttctaa catttgacct
    36121 gagattcttg tatcccgccc tactaacacg cgtgggcgtt ttccttcttc ttcatgctgg
    36181 cttaggacat aacctccgca ccgtcccagc ttaaatgcaa gttctggcgt aagttccttg
    36241 ttggcttcac cgcgtacgcc gtctgttcca aaatatttac ccatagttgt aaaattactc
    36301 ctttaactta attttctgag gaatttgttc tgctcatttg tgttgattcg cttgtttctg
    36361 atgtttcttc tgtacttgtg gtcgagctgc ttgtcccaga ttccgaactg gaagaaatgg
    36421 ttgttgtcga tggttccgtt cctgaatcag cagatgaaat acttctactc gtacttgtat
    36481 tttcttgtgt ccgactgctc gtcgaagcag agctactgct tgacgtttgc aacacgggtg
    36541 taatctccac actaactgat tcgggcttga tacttaaccc ctcttctact ggtatttcaa
    36601 ttttacgagt tgttcgatga ttgatacctg agacattgat cggtacagca atactgtcac
    36661 cgatcgatgc aagcaaatcg ctagagccgc ttacttctgc ttccagtttt gaaaaactga
    36721 aatcatagct ttctaccccg gcagctggtg ttccttgctg tatcccataa agtcgtaccg
    36781 acttgcttgg cttgatcgaa tcagctctca ctgttacttg cactggatca gagacgatac
    36841 ttaatacttc ccctgctgca ttcagcgcct gtattgggac ctcactatta atcccatcat
    36901 ctgtgaccat cgaagcgtcc acatcagcta ctactcgttt gatttctgcc aatgttttgt
    36961 cacctgtagt gatagagact tttttaggac taacggaagt atcatcaagt tcatatccag
    37021 atggtgtgaa cgttgaagga atcaacgttt ccacatcgaa atcttttatt acctttttct
    37081 caaccgttac tgtaatagtc gatggttcga tctttgctgt gacagcactg ctcaaatttt
    37141 gaatttttag cggaacttca tgtgttcctt cgcccagctt agtgatatct gctgtgacgc
    37201 ggaacatccg tgtatcttcg tttgcctctg cattcaactg aactcggttt gcactggaaa
    37261 gtttgacagt cactgcattt tcatatccat ggatatagta gcgctttgaa tcataagaaa
    37321 tcttgatcgg cacccccgta actgtttgat tatacgcttc atttccagaa agcgtatttg
    37381 gaacggactg gccatttgca ttgaagtaca gcgcaatact gaacagcaat gcaataaaac
    37441 tgtaaagaat gcttgtccgt ttctcttttg agatcatttg cgatcccctc ccttagtcat
    37501 accgtctagg aaagattgaa tcacgttttt cttgtttttt gttttctctt cagcaattag
    37561 ttctctacgc aagattgcca aatattcttg ctgggataag ccagccatca gttcattatc
    37621 taacgtgatg ctgacgtccc cggtttcttc agagacaacg atggtaatcg cgtcactgac
    37681 ttcgcttata ccgacagctg cccggtgtcg agtcccgaat tctttaggga tcaacatact
    37741 ttctgataga ggaagatagg cgcttgctac tgcgatcttt ccttctttta caataactgc
    37801 accgtcatgc agcggtgtat taggaataaa gatattgatc agcagctccc cagtgatatc
    37861 tgcatctaaa gcaatccctg tctcaatata ctcatctaat ccagtatgac gttcgatagt
    37921 gatcaacgca ccgatttttc gcttggacat atattggatg gctttgtcaa aagacaagat
    37981 catcttctca tcttcctgct gttcgctttt tgattgtttg aaaagcgaac tgcgtccaag
    38041 atgttccagt cctctccgga tttccggctg aaaaatcacg atagcagcta tcaccccgta
    38101 cgtaatgact tggttcatca gccaagacaa ggtgtgcaga ccaatgattt ccgctacgat
    38161 tcggataaca ataaaaatag ctacaccttt cagaagctga atcgcttttg tgcctcgcag
    38221 cagttggatc agtttgtata caagatacca gacaaccaga atatcaagta tgttcaccaa
    38281 aaaattcaga gagaacaagt caccggaaaa catttgctgc caatagtcca aattaaaaag
    38341 ttccccgatt tgaaatgaca taaaaagcct ctcttctctt ttgagatttc ctctctagta
    38401 tatcatatgt tttaatttta gaggctgata tgctcgtatt tttcttgaga aaaacgcata
    38461 attttcaaga aaattgcaag tatgcgtttt atttttctct gcaataaata tttttaaact
    38521 tcagagaatt attcattata ttttttctgt tatttttcct ttttttcttt tgtagcttgt
    38581 tccatttttt tacgtcgttc tgctctggca gctcttctag cagctcttct ttcttctgct
    38641 tcaaggtccg tcgttttttc ttttctagtc ttaacgggta catcgataga ttcctctggt
    38701 tcaagtcgtt tcttgatttt ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt
    38761 gttttagcgt cgtggacagt cttttcaaat aaagcttttg caagatttgg atttgcgtta
    38821 tctagcgagg aaaagcgaac ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt
    38881 tttttgtagt ccaaagtcat tgggttcttg tttttttcga ccattgtagg gttatatcga
    38941 tataatgacc aataaccaga agaaacagct tcttttgctt ctttaagcga ttctttcatt
    39001 ccgccaagca agccatgtgt gatacaaggc gtataggcaa taatcagaga aggaccagga
    39061 tacttttctg cttcttcaaa tgccttgatc gtttgcattt gattggcacc tgaagcaatt
    39121 tgcgccacat aaacatttcc atatgtcatc gccatcattc ctagatcttt ctttgaagca
    39181 tactttccac tagccgaaaa tttagcgatt gccgaagctg gtgttgcttt tgaggtttga
    39241 ccgccagtat tggaatagac ttcgttatcc aaaacaagga tattcacgtc tgctccgctg
    39301 gcaagtacat gatcgatccc gccaaaacca atgtcgtaag cccatccgtc tccaccaatc
    39361 atccattggc tgttttttac gaatagatcc tttctcttgt atatatcctc cagttcaggg
    39421 aaaattgatc gttcgctttc gatcgcagct tgcagttttg ctgctctttg ctgcgttcct
    39481 tcactctcaa acatgtgtac taaccagtct tccatcaatt ctcgtagttc ttctgatact
    39541 tttggcaata cttctttgat tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga
    39601 agcataccat aaccaaattc agcattatct tccaataaag aatttgacca tgcaggccct
    39661 tgaccacatt cattggtcgt ataaggagaa gcaggagaag caccgcccca aatagaagaa
    39721 cagcctgtcg cattcgcgat catcattcga tcaccgaaca tttgtgtcaa taattttaca
    39781 taaggtgttt cgccacagcc tgagcaagcg cccgagaatt caagaagcgg ttgattgaat
    39841 tgtgtagaca agacagtatt ctttcctttg actggatttg ctttttgtct taatgtcatt
    39901 gcaaaagccc aattcacagc ttgttctttc tgttcttcat aaggcttcat cactaaagct
    39961 ttttcttttg cgggacaagc ctctacacac agaccacaac ctgtacagtc ttccactgaa
    40021 acttggatac ggtattttac gccgtctgcg cctcgcattt ctctcacgat aaatccttct
    40081 ggcgcttctt ccatttcctc ttcatctgct aaaaatggac ggatggcagc atgaggacaa
    40141 acaaaggcac attcattaca catcgtacag cgatcactaa tccactcagg tacttctaaa
    40201 gcgacgcccc gcttttccat agaagccgtt cctaaaggca tttcgccagt tgtcattcca
    40261 tttgctacaa gtgtaccaac agagagttga tttccttcct gtgcattcac tggttcaagt
    40321 atttcttgta tataaccagt gttcctttta ttgatttttt caggcatctc caacgttctc
    40381 caatggtctg ggatcgcgac ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg
    40441 tttttttcta cgatagccat tgatttatgt ccatagcttt tcagggcttc ttcttttagc
    40501 agcggaagca cctcatcaaa aggaatgatg tcagctaatt taaaaaaagc cgtttccatt
    40561 gccgtattga tccggcgtcc caaaccgact tcttgggcca atctcatggc attgatcgta
    40621 tagaatctaa tgttattttc agccaagtaa cgttttaatt ttaagggcag atgcgtttct
    40681 aattgttcgt cgctccaaag agtattcaat aaaaaaatgc cacctggctt caatcctttg
    40741 accaagtcat agctatgcag gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg
    40801 acaagatacg ctgaacgtat cggcgtatct ccaaaacgaa gatgagagac agtcagaccg
    40861 ccagattttt tcgaatcata ataaaaatag ccttgcgcat atttatcggt gtgatctcca
    40921 ataatcttga tagctgattt atttgctcca actgttccgt cagatccaaa tccccaaaac
    40981 ttggcctgga atgtttgcgg ttctgtcaaa tccagacttt cctttggttc caaagattga
    41041 taagtcacat catccacgat ccctatggta aaccgttttt tgcgtatgct tgggtctttt
    41101 ttcaattcat caaaaactgc tgagatttgg tcgggagtca catctttaga ccccaatccg
    41161 tagcgaccac caataacagc cgggcgaagt tcagaatcat ataacgcact ttgaacgtct
    41221 aataagagag gttctccacc tgcccctggt tctttacttc gatcaagaac agcaattgtt
    41281 tttactgtat gaggcaactt ttgcacgaat gtttccactg gaaaaggacg ataaagatgg
    41341 atattcaaaa aacctgtttt ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc
    41401 gtctgtgcaa ctgatcccat agaaacaatg acttcttccg catcgtctgc tccgtaataa
    41461 gtcaccaagt cataagaagt tcctcgtaat cggttgatct ctttcatata tttttgaacg
    41521 atttcaggta atttatcata ataggggttg atcgtttccc gttgttgaaa atggatgtct
    41581 ggattctggt tcgtaccact aacagtcgga tgattgggat tcatccctcg tttacgaaat
    41641 gaagctaatt tctcctgatt gatcagagga gctaattctt catattccaa aacttcgatt
    41701 ttttgaatct cgtgactcgt acggaatcca tcaaagaaat tcaaaaaagg gacttctcct
    41761 tcaatagaag caagatgggc aacaggtgct aaatccatca cttcttgtac actgctttca
    41821 gctaacatag caaatcctgt ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata
    41881 ttcaacgcat ttgtcgtcac tgcccgacta gcaacgtgga aaacacttgg cagtagttcc
    41941 ccagcaatct tgtacatatt cggtatcatc aaaagcagcc cttgtgaagc ggtatatgta
    42001 gttgtcagtg caccagtttt caatgatcca tggaccacac cagctgctcc tgcttcggat
    42061 tgcatttcta ctacttttac tggttgaccg aatacgtttt cttttcctgc tgctgaccat
    42121 tgatctacta attcagccat ggtcgaactg ggtgttattg gatatattgc agcaatctcg
    42181 gtaaatgcat aagaaatata tgcggctgcc gtattgccat ccattgtttt catttttcgc
    42241 atgattcatc atccttgttc atattctctc ttattatctg ttccattata acgtaaaaca
    42301 tgccattttt ttcttttatt tataaaaata ttttacatgt atctatttga attggtcatc
    42361 taatttttag acaaacagtc taaatcgttg cctttttttg tcagacatgt ataaactata
    42421 agcgaaagag gtgttttaac atggatatat cagtgattga tgcaacgaaa acaaatacac
    42481 aaaaaggaat cctttatggt tcatcgaatg ctcctaaaaa aatggtcgaa ttcatcaact
    42541 tagcttgtcc gtattgccga caatggtttg aagaatctta tgagctatta gaagaagcag
    42601 tccaaagcgg gcaattgcag cgagtgatca aattgttcga taaagaaaaa gaaagcttac
    42661 agcgagggaa cgtgatgcac cggtatctca caatcagcga tggccaaaaa gcgatcaaag
    42721 agatcaaaca aatattcgat actcaagacg agtggaaaca tctttctctc caagaagtag
    42781 cggattttgc agtagataaa ctaaagctga cagaacaaaa agacgaacag ctgagccaag
    42841 ctgtcatcaa cgaagctgag caagcacata ttcgatttgt tccaactgtc atcttgggta
    42901 aagaaatctt tgatgaatct atttcaatag aggaattaaa agaattgatc caaacaaaat
    42961 aaaaaaataa ggagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
    43021 tataaacggc ggaagcagaa gcaactcctt cttatttctt ggagttaaac gcttctgtcc
    43081 cagcctcctt attccgaata aatggtgtcc aaaagcttgc atctgccaga ttatctcatt
    43141 tcttatgaca agctaagcaa attatctcca ccaaataatt tattcatatt tttgacttgc
    43201 gcatactaac taattcttaa attttcatac aaaatttctc aatttccctt aacgagtaat
    43261 atttatgtac ggtgattctc actagtatcc caatcacttt ttaagttatc ccgatttcaa
    43321 ttctacttca aaaatagttg cttttatgta tgctgttagt tgattagatg gtcatttgtt
    43381 gagctaggtc gtttatttta aacaagttgt ttctttagct tatacctatc tcgtctacca
    43441 aacgcaagat ggcaaacaca aatgcatacg catctgtatt tgccactttg attcgtataa
    43501 agctaacaac tattaactcc tctcagctaa tagttgctct cttttttatt cagattcatt
    43561 tttgctgatc acagcctagt atttcaatgg ttttgaacac taaaccaaat tgttccatta
    43621 caacattcac tggctgatga aatggattag tagatttcga ttattcaaag cgattttcta
    43681 ttggtggatc gtgttcccaa ctaataagac ggattccaaa cctatcgtac agatcttcta
    43741 aaccacttat aaattttgca cctatttcag gtgttttatt gcgtggatat tctattgtta
    43801 cgccattaaa tggataccaa ttaggattag gctctctttt tttctgtaca tctcccatag
    43861 aatagttagc actttctggg acataatata ttcttcgacc atcctctaat tcatctaata
    43921 agatatttcg tgatcgtcta tcacttgcta aatgtagacg ccacgtgcga taccatgcca
    43981 tgctacctgc atctgcagaa aatccatcaa ataatgcaac aattgtatct tctatcacaa
    44041 tcctttcccc atctacttca tattctaatc taaaatcaaa ctcccctcgg gtaatttctg
    44101 gtcttggtgg cttaggaccc aatattattc tacttaaaac cgcattcgtt cccagatgaa
    44161 acacagctat aacaacaata atgattaata ttttgtttct atttgataaa tttctcatta
    44221 ttcgcacatc acctctacat tctcacatat tagttcgtaa tactccttta atatcaaaat
    44281 atctactggt cgtaataata ttcgctatga attcctacta ctcgtataag aaaggccaaa
    44341 tcatcagctt ttacttgctg atgatttggc ttgtttctat ttatcgtaat tgttttctaa
    44401 tcaccaaaat tgttaacaat cggttcagat atttcccagt tgattaaggt aattccgtat
    44461 tgtgtcaaaa cttcttgcgc ttctccgaaa ttaagtacag ttcttatgta tccactttct
    44521 cctaatataa agtctacctc tcttttaaaa ccttcctcga gatttctaag ggctatatgt
    44581 ggttttaatg acattgattg tacattttcc cccatataat attgcgcact tcctcgacta
    44641 aaatagattc ctagatcgtc cccaaaaggt agatcttctc taccactagc taatctactt
    44701 ctccatcttc gttctctttt catagaacct gcgctgaact cacacccgtt aaactcagct
    44761 atgacagtat cttcaattat gttaatctgt ccatctagtt cgtattcaat cctaaatgga
    44821 aattctccat aggtgatttc cggttcgggt attttaggtg agattaatgc aaagaaagga
    44881 aatattaagc ttggcaacat caaaaaaact cctcctatga tcacaattcc cgatgtaatc
    44941 caaaatataa tttgtacaat tctcattgat ccattgtatt ccattttatt ccccctatta
    45001 ctattcatat ccgcctatct atatcgatct tccaatgcag agataattgc ttccatacct
    45061 tgatttttta ctgagttacc tatgcgagat atcattctcc actaaggtgc atcatattgt
    45121 ctatctaaca tgattaattc tccaataggt gtatactcct ccaaaaacaa catttaatgc
    45181 atctccctcg acaacataag ctaccatgtt tgctgtatat gtgtttgagt ctaattcata
    45241 agcagataaa tttactgtcg ctggattaaa tataatcgca ttcgtattta tagctactga
    45301 atttgctgct tcagcaccac cttttgaatg tccgatcatt gtgatttcaa tagtaggatt
    45361 tacgtggaca gccctcaccg tttaccctga ccattataga aatcgcttac ttttttgaac
    45421 tacgcaacaa aggatacaat gctcctaact tcactgtctc catttttgtc cactaaggaa
    45481 tataaaaaat aggcattaat tcatttaatt cggtcacaac acgttacctt ttaaatcatt
    45541 atactgtatc tcatacatta ttatcaataa actattataa aaatgcaaaa aatgctttat
    45601 caatctattt tgtaattggt cataatatca ttgaaatcat accctgtatc gatttttcct
    45661 atgaatctcg tctatttgtg attgacgaaa aagaggcaag acagacactg gaatggtaat
    45721 tttacccact attttgctcc aactatttgt aatcgtaccc acttttgaaa taccgagatg
    45781 atcattttaa ttgatatgct gattcccaag caaaacccca agaaattaaa tgctctatcg
    45841 catctagctt cttgggtttt gcttatataa attccaacat ttaaattttt tatgctgttc
    45901 ggtctcagaa atgccgtttt tgcttatatc acaatatcaa gatgtcaaat tattcgtgtt
    45961 gccgatgact ttgattaatt tttttcaaat tgtttattaa gactaatgct gtctttttgc
    46021 agataccaat gttcgaactt tttaacatta tcatatgatc ctgcaagttt taacttctta
    46081 ccattagatg ttatcaaagt tataattaac cttgcctttt tcccattagg atcttttatt
    46141 ttatgaattt tctgcaggta atctctccta atggcagcta tttttacctc atcagtgctg
    46201 ataaaccatt gatcatccac atacatactg cctgttatgt tgtgtttttc catttcttcc
    46261 gagaacgaaa agtttaaggc tcgttcttac ttccaaagtt gaatgagtcc atggattgga
    46321 cttagtgtga gaattgagaa caacaataag aatccatatc ctcccgcaaa catccctaag
    46381 gctatactgt ctctccatcc ataagtagga ttagaagaga aaagagtcac cgtttgtaat
    46441 aatatgaaaa ggaataatcc agcacaaaca ccaaagataa caactccaaa cattgaattt
    46501 ttatacaatg accatttatt tataggcttt tctttcattt tacttccccc tactgttctg
    46561 ttcacacgga atgaattcaa agttcataag ccctcctctt tgaacatatg aatacgactt
    46621 gctaactata ctttaaaaaa gcttgtttta agcggttata ggcgagttgt aatctctatt
    46681 catctcatta gcaaataact tgccgaaaag tctactgaaa ttaaagcaga tagtggaaga
    46741 attatttagc caggatagac actatttgtg aaacacatgt ctttactttt gaaattctat
    46801 gatgaaaagc cttggaatac agtagctctt aattggtaat actttttcca ttataaataa
    46861 cctcttttca ttttatattt atcccctcta aaaataatat ctaatctaat attttaaact
    46921 tatatttccc tcgctgtgct aatagagcat tactaaataa atagaaacta gaaaaaagca
    46981 ttacgcctgc cagaataaac caattatttg t
//
LOCUS       DAJSAW010000019        44918 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJSAW010000019 DAJSAW010000000
VERSION     DAJSAW010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.019"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            211..603
                     /locus_tag="ONT64_001141"
     CDS             211..603
                     /locus_tag="ONT64_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR2302573.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            631..1971
                     /gene="glnA"
                     /locus_tag="ONT64_001142"
     CDS             631..1971
                     /gene="glnA"
                     /locus_tag="ONT64_001142"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCR2302574.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3164..4774
                     /locus_tag="ONT64_001143"
     CDS             3164..4774
                     /locus_tag="ONT64_001143"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCR2302575.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5066..5935
                     /locus_tag="ONT64_001144"
     CDS             5066..5935
                     /locus_tag="ONT64_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCR2302576.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6100..7371
                     /locus_tag="ONT64_001145"
     CDS             6100..7371
                     /locus_tag="ONT64_001145"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR2302577.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7368..8663
                     /gene="rho"
                     /locus_tag="ONT64_001146"
     CDS             7368..8663
                     /gene="rho"
                     /locus_tag="ONT64_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR2302578.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8762..9025
                     /locus_tag="ONT64_001147"
     CDS             8762..9025
                     /locus_tag="ONT64_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCR2302579.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9470..9919
                     /locus_tag="ONT64_001148"
     CDS             9470..9919
                     /locus_tag="ONT64_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2302580.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9976..11394
                     /locus_tag="ONT64_001149"
     CDS             9976..11394
                     /locus_tag="ONT64_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2302581.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11394..12254
                     /locus_tag="ONT64_001150"
     CDS             11394..12254
                     /locus_tag="ONT64_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR2302582.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12428..13201
                     /locus_tag="ONT64_001151"
     CDS             12428..13201
                     /locus_tag="ONT64_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR2302583.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13348..13713
                     /locus_tag="ONT64_001152"
     CDS             13348..13713
                     /locus_tag="ONT64_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HCR2302584.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13861..15069)
                     /locus_tag="ONT64_001153"
     CDS             complement(13861..15069)
                     /locus_tag="ONT64_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2302585.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15088..15366)
                     /locus_tag="ONT64_001154"
     CDS             complement(15088..15366)
                     /locus_tag="ONT64_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302586.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15520..16215
                     /locus_tag="ONT64_001155"
     CDS             15520..16215
                     /locus_tag="ONT64_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR2302587.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16657..17454)
                     /locus_tag="ONT64_001156"
     CDS             complement(16657..17454)
                     /locus_tag="ONT64_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HCR2302588.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17451..18635)
                     /locus_tag="ONT64_001157"
     CDS             complement(17451..18635)
                     /locus_tag="ONT64_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCR2302589.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18660..19907)
                     /locus_tag="ONT64_001158"
     CDS             complement(18660..19907)
                     /locus_tag="ONT64_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCR2302590.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20122..21867
                     /gene="dnaX"
                     /locus_tag="ONT64_001159"
     CDS             20122..21867
                     /gene="dnaX"
                     /locus_tag="ONT64_001159"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR2302591.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21887..22201
                     /locus_tag="ONT64_001160"
     CDS             21887..22201
                     /locus_tag="ONT64_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR2302592.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22226..22822
                     /gene="recR"
                     /locus_tag="ONT64_001161"
     CDS             22226..22822
                     /gene="recR"
                     /locus_tag="ONT64_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR2302593.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22886..23530
                     /locus_tag="ONT64_001162"
     CDS             22886..23530
                     /locus_tag="ONT64_001162"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR2302594.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23545..23874
                     /locus_tag="ONT64_001163"
     CDS             23545..23874
                     /locus_tag="ONT64_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCR2302595.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23888..24826
                     /gene="holB"
                     /locus_tag="ONT64_001164"
     CDS             23888..24826
                     /gene="holB"
                     /locus_tag="ONT64_001164"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR2302596.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24862..25686
                     /locus_tag="ONT64_001165"
     CDS             24862..25686
                     /locus_tag="ONT64_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCR2302597.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25679..26026
                     /locus_tag="ONT64_001166"
     CDS             25679..26026
                     /locus_tag="ONT64_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCR2302598.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26095..26967
                     /gene="rsmI"
                     /locus_tag="ONT64_001167"
     CDS             26095..26967
                     /gene="rsmI"
                     /locus_tag="ONT64_001167"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR2302599.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27076..28197
                     /gene="dinB"
                     /locus_tag="ONT64_001168"
     CDS             27076..28197
                     /gene="dinB"
                     /locus_tag="ONT64_001168"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR2302600.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28251..28853)
                     /gene="nrdG"
                     /locus_tag="ONT64_001169"
     CDS             complement(28251..28853)
                     /gene="nrdG"
                     /locus_tag="ONT64_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCR2302601.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29168..31357)
                     /gene="nrdD"
                     /locus_tag="ONT64_001170"
     CDS             complement(29168..31357)
                     /gene="nrdD"
                     /locus_tag="ONT64_001170"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR2302602.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKNQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31656..32450
                     /locus_tag="ONT64_001171"
     CDS             31656..32450
                     /locus_tag="ONT64_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2302603.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32440..34482
                     /locus_tag="ONT64_001172"
     CDS             32440..34482
                     /locus_tag="ONT64_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2302604.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34763..34912
                     /locus_tag="ONT64_001173"
     CDS             34763..34912
                     /locus_tag="ONT64_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCR2302605.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34923..36437
                     /gene="dltA"
                     /locus_tag="ONT64_001174"
     CDS             34923..36437
                     /gene="dltA"
                     /locus_tag="ONT64_001174"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCR2302606.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36434..37639
                     /gene="dltB"
                     /locus_tag="ONT64_001175"
     CDS             36434..37639
                     /gene="dltB"
                     /locus_tag="ONT64_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HCR2302607.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37684..37917
                     /gene="dltC"
                     /locus_tag="ONT64_001176"
     CDS             37684..37917
                     /gene="dltC"
                     /locus_tag="ONT64_001176"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HCR2302608.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37920..39188
                     /gene="dltD"
                     /locus_tag="ONT64_001177"
     CDS             37920..39188
                     /gene="dltD"
                     /locus_tag="ONT64_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCR2302609.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39329..40411
                     /locus_tag="ONT64_001178"
     CDS             39329..40411
                     /locus_tag="ONT64_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCR2302610.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40411..41214
                     /locus_tag="ONT64_001179"
     CDS             40411..41214
                     /locus_tag="ONT64_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCR2302611.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41284..42024
                     /locus_tag="ONT64_001180"
     CDS             41284..42024
                     /locus_tag="ONT64_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR2302612.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42035..43039
                     /locus_tag="ONT64_001181"
     CDS             42035..43039
                     /locus_tag="ONT64_001181"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCR2302613.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43084..44847)
                     /locus_tag="ONT64_001182"
     CDS             complement(43084..44847)
                     /locus_tag="ONT64_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302614.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14441 a         7833 c         9346 g        13298 t
ORIGIN      
        1 ataaaaacac taataaagga tagtacagta taataaaaaa taaaaggtat ctggtacgaa
       61 aatcaactag ataccttttt tgtgttctta tttttttgtt attttataaa aaatagagat
      121 ctcatgttag aaaacctaac atatgcagtt gacaatattt tttttaattg gtacacttta
      181 gccaacttat aaaacgaaag gggacgtctg atgggagaaa aggaattgcg ccgatctatg
      241 tccgtatttc caattggtac agttatgaag ctgactgatt tgtctgctcg gcaaattcgc
      301 tattatgaag aacaagattt agttcatccg gaacgaagcg aaggaaaccg acgtatgtat
      361 tcgctgaatg atattgatat gttgcttgag attaaagact atctttcaga gggattgaat
      421 atggctggga tcaaacgtgt atacgaaatg gcacaggcga aaaaacaaga agcaaaaaac
      481 aagaagccgt tgacagatca tgacgttcga caaatttttc gtgatgagat tttggcgcaa
      541 ggtgggttaa gcaaaacagg tcattatcag tcgcaaggat tgcaaagaca attctttgac
      601 tgaataaatt aaaaaaggaa gagggatacc atggtaaaga aacagataac aggtgaagaa
      661 atcaaacgaa tcattgagga agaaaacgta cgatttttac gattgatgtt cacagacatc
      721 ttaggtacga tcaaaaatgt agaagttcca gtgagccaga tcgacaaagt attagaaaat
      781 aaaatgatgt ttgacggttc ttcgattgaa ggatttgttc gaattgaaga aagcgatatg
      841 tatttgtatc cagatctctc cacttggatg atcttcccgt gggaaagtgc tcatggaaaa
      901 gttgctcgtt taatttgtga tatctataat ccggatggga caccatttgc cggcgatcct
      961 cgcgggaatt tgaaacgagc attgaaagat atgcgcgacc ttggttttac ctctttcaat
     1021 ttaggacctg aaccagaatt cttcttattt aaattagacg aagacggtgg gatcacaacg
     1081 accttgaatg ataaaggtgg ttattttgat tttgcaccaa cagatttagg tgaaaattgc
     1141 cgccgtgaca tcgtgttgga attagaaagt ctaggctttg aagtggaagc ttcacatcat
     1201 gaagtagcgc caggacagca tgaaattgac tttaaatatg cagatgtggt agatgcttgt
     1261 gataatatcc aaaccttcaa gctcgttgtc aaaacgattg cacgcaagca tggacttcac
     1321 gcgactttca tgccgaaacc gttatttggg atcaatggtt ctggtatgca ttgcaatatg
     1381 tctttgttca atgatgaagg aaatgttttc tatgataaag acggtgaatt ggaactaagt
     1441 gaaacagctt atcatttctt aggtggtttg ctgaaacatg ctcgtgccta cacagccgtt
     1501 tgcaatccaa cagtcaattc ttataaacgt ctcgttccag gttatgaagc gcctgtttat
     1561 gtcgcatgga gtggccgtaa tcgttcgcca ttagtacgtg taccagaatc tcgcggttta
     1621 tccacacgtt tggaattgcg gtcagttgat ccatctgcaa acccatactt ggcaatggca
     1681 gtattgttgc aagcaggact agacggtatc cgtaatcaga tcacaccgcc agcagctgtt
     1741 gaccgtaaca tttatgtaat ggatgaggaa gaaagagaag ctgcacatat ccaggactta
     1801 ccttcaacta tccataatgc tatcaaagaa ttgcgtaagg ataatgtaat gattgacgca
     1861 ttaggacaac atatcttctc aaattttgtt gaagcaaaac gtctcgaatg ggcggcattc
     1921 agacaaactg tttccgagtg ggaaagagaa caatatctag aactttatta aaacaggaat
     1981 accgtgaatt agaggctgtg aaatcagcgt tttgacctga gtattagaga aactaacgga
     2041 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag ctgattgaac
     2101 accgtttagt ggagaggttg tgaaaaagct gccctgcaac aagtaataag aatagccaag
     2161 caaaaacagc ttttcttgtt tttgaattgg ctgaacttat taccgcagat gcaagctttt
     2221 gaacaccgtt taccaagagg ttttgaaaaa gacgtttagt ttcgagtatt aagaaaaatt
     2281 ttgtgaaaat ggctttctac atttttgcat aaatttggct taataccgag aaactggctt
     2341 ttgaataccg tttatccaga ggttgtgagc ctaccattta actccgaatc acaagaaata
     2401 atttcaaaaa cagcttctca tgtttgatgg atattgtgac ttgtgaccga ggagttggca
     2461 ggagaacacc gtttatttag aaataaggag gcacgacttt tgtcgcatct ccttattttt
     2521 tacttatgtg attcgttacc ttcaatgaat ccctacaagg cacttttgtt gaaaaaaagg
     2581 tcatctttga aaaaattagt ggaattgttt tgtttttgat attatttaat agaaaagata
     2641 tgccaaaaat ccgagaactg tggactacgt tttttacgta aaggaggtaa ataagggtga
     2701 aagaaaaaga ttgtttctac gataagctat ataaactgtt gctgcaagta ggaaaaagta
     2761 gttctttgtt aggtgggata ttatctatca taatgtttgt tttatttttt tatctattat
     2821 ttcacttaaa atttttcagt aacgattttc tgatttgctt aggtttaacg ttagctatgt
     2881 ttatatcaag cttattatgt gggttaaatt ttttttgcac tcataaagga cttaagaaat
     2941 aatttctcaa aggatatcga aaaagaaaaa attgtaagcg gatagtattc atttgagtac
     3001 ttgactttcc cttagtcagc ttgtattata caccttgggc acccttttag agggtcagaa
     3061 gtttacagaa atttcggctc cctattctta ttgaagaata gggagctttg tttattgtct
     3121 gagacttttc ctgcacataa attaaaaagg agtttttgaa cttatgacaa aatatatttt
     3181 tgtgaccggc ggtgtcgttt catcaatcgg aaaagggatt gttgcagctt ctttaggacg
     3241 cttattgaaa aaccgcggct taaaagtaac tatccaaaaa tttgatccat acatcaacgt
     3301 ggatccgggg acgatgagcc catatcagca cggagaagta ttcgtaacag atgatggtgc
     3361 tgaaacagat ttggatctgg gccactacga acgttttatt gacatcaatt tgaacaaata
     3421 ctccaacgtg acgacaggaa aaatctattc tgaagttttg cgtaaagaac gtaagggcga
     3481 atacttggga gcaactgttc aagttattcc tcatattaca aatgagatca aagaaaaaat
     3541 catgcgagca gctaaaatga ctgattcaga tgtgatcatt acagaagtag ggggaacagt
     3601 aggggatata gaatctttgc cattccttga agcacttcgt caaatgaaag cagatgtcgg
     3661 tacagataat gttatgtata tccatacaac attgattcct tacttgaaag ctgctggaga
     3721 aatgaaaact aaaccaacgc agcacagtgt aaaagagcta cgtggtttag gtatccaacc
     3781 gaatattttg gtggtacgta cagagcaacc tgtttctcaa agcgtgaaaa acaaattggc
     3841 acaattttgt gatgtcgaac cagaagcagt tatcgagtcg ccagatgtag atacacttta
     3901 ctccatccca ttagttttac aagcacaagg aatggatcaa attgtttgcg accatttaaa
     3961 actagataca ccagaagcag acatgacaga atggaaagaa ttagaagaac gtgtcttgaa
     4021 cttgaagaaa aaagtacgga ttgcgcttgt cggtaaatat gtcgagttac cagatgcata
     4081 tatctcagta gttgaagcat tgaaacattc tggtttcgcc tataattcag acatcgagat
     4141 cgattggatc aaagcacaag aattaacgag agaaaatgta gaagaacgtt tgaaagacgc
     4201 agatggaatc ttggttcctg gtggatttgg tgatcgcgga gtagaaggga aaatcgaagc
     4261 gattcgttat gctcgcgaaa atgacgttcc gtttttaggt atttgtcttg gtatgcagat
     4321 ggcttgtgtc gaatttgcac gtaacgtcgt tggattagaa gatgcgtcat cagctgaaac
     4381 gaatccagat acagcaaaca atattattga tttaatggct gatcaggaaa acatcgagaa
     4441 tttgggtgga actttacgcc ttggattata cccatgtaaa atcaaaaaag gaacgaagac
     4501 ggctgaagca tatgatggag ccgatgttgt gcaagaacgt caccgtcacc gctatgagtt
     4561 caataacaaa tatcgtcaat tgtttgaaga acaagggcta gtcttttcag gtgtctcacc
     4621 agataaccgt ttagtagaaa tcgtagagct gccagagaaa aaattctttg tgggatgcca
     4681 attccaccca gaattgattt ctcgtccaaa tcgcccgcaa aaattaatca aagcatttgt
     4741 cggagcatca ttagcagatc acgaaacaaa ataatttttg aacttgatag aaagagaaaa
     4801 gtagcaagag aaggggataa gcaattttct tgtcactttt ctctttttct gatttgttat
     4861 tatttgtagg aaaaaagata gaaagcaaaa gacttttttc gattaaagcg ctattggata
     4921 ttttattttt tgcaaaaaac ttcttgaaaa ttttcatttt cattgatttt agtagaaaac
     4981 taccgaaatg tttggtaaaa tagggtcgtt ggttaagaat tgtcttaaca aatttagttt
     5041 tacaaaactt aggaggaatt ttattatgcc agtagtatca ggagctgaat ttttaaaagc
     5101 tgctcgtaaa ggcggttacg ctgtcggcgg attcaacaca aacaacttgg aatggactca
     5161 agcaatcctt gaagcggctg aagcaaaaca agcaccagta cttatccaaa catcaatggg
     5221 tgcagctaaa tacatgggtg gttacaaaat ctgtaaagat ttaatcactg atttagttga
     5281 agcaatgaac atcactgttc cagttgctat ccacttagac catggtgatt acgaagctgc
     5341 tttggaatgt atcgaattag gctatacttc agtaatgttc gatggttcac atctaccatt
     5401 tgaagaaaac ttgaaattag ctaaagacgt tgttgaaaaa gcacatgcaa aaggtatctc
     5461 agttgaatgt gaagttggtt caatcggcgg agaagaagac ggaatcatcg gtaacggcga
     5521 attagctgat attgaagaat gcaaacaaat ggttgctaca ggtatcgact acttagcttg
     5581 cggtatcggt aacatccatg gtcaatatcc agaaaactgg aaaggattag cttttgacca
     5641 cttacaagca attgctgatg ctgtaggtcc agacatgcct ttagtattac atggcggatc
     5701 aggtattcct caagatcaaa tcgaaaaagc tatctcaatg ggtatctcaa aagtaaatgt
     5761 taacactgaa ttccaattat catttgctgc agctactcgt aaatatatcg aagctggtaa
     5821 agataaagaa ggtaaaggat tcgaccctcg taaattatta gctccaggta aagcagcaat
     5881 cgttgaagat gctaaatctc atatcgactg gttcggttca gctaacaaag cgtaagcatc
     5941 cacataacag atacgatata taggaatgga gaacgacttt tgaagtcgct ctccattctt
     6001 ttttacttaa aacggcagac catgctcgtt tctttttttt atctatgata aaatacaagc
     6061 atatccatga aaaaagatta ataaacaagg tggaaacaga tgaaaaaaat cgtaatcaaa
     6121 ggaaaccgac ctttagctgg tgaagtaacg atcagcggtg caaaaaatag tgcagtcgca
     6181 ttgattccag ctgcaatctt agctgattcc ccagtgatct tggaaggtgt gccggacata
     6241 caagatgttc attcattaat tgaaatttta gagatcatgg gcgcaactgt ccatttttcc
     6301 aacaacatac tagaaatcga tcctcgagaa gtcgtatcga ttccgatgcc gcacggaaaa
     6361 atcaatagtt tgcgtgcttc ttattatttt atgggaacac tgcttggtaa atttggcgaa
     6421 gcggtagtag gcctgccagg tggctgttat ttaggcccta gaccgattga cctacacgtc
     6481 aaaggatttg aagctctagg tgctgaagtg acaaatgaac atggtgccat gtatttacgg
     6541 acagaaaata aaggattgcg tggcaatcgg attttcatgg acgtggtttc agtcggagca
     6601 acgatcaatg tgatgttagc tgctgtaaaa gcaaaaggac agacagtaat tgaaaacgct
     6661 gcacgagaac cggaaatcat agatgtggca actttactga ataatatggg ggcaaaagtt
     6721 cgaggagcgg gtacagatgt gatccgtatc gaaggagtcg aaacattaca tggctgtcgg
     6781 catttcatga ttcctgaccg catcgaagca ggaacctatc tagcattagc agctgcagta
     6841 ggaaatggta tcaaagtgaa aaatgtgatc tttgaacatt tggaaagctt tatagcaaaa
     6901 ttgcaggaaa ttggtgtcca tatgaaaatc agcgaagatg aaatcgaagt ttacccctcg
     6961 aaagaattaa aacccgcaaa catcatgacc tatccttatc caggatttgc cacagatctg
     7021 cagcagccac ttactgcgtt actattgatg actacaggca cttcagaaat catcgatacg
     7081 atctatgcga aacgtgtgaa ccatgtgccg gaactagcac gaatgggcgc tgatattacc
     7141 gtggaaggaa atatgatcat cgtgaatggt ccaaataaac ttcacggcac agaagtcgtg
     7201 gcttctgatc ttcgggcagg tgcttgttta gtgattgctg gactattggc agaagggaca
     7261 acgacgattt acaatgtaga ttatatctta cgcggttatg atcatattat cgagaaacta
     7321 acagccttag gggcatcaat tgaaatgata gaggaagtgg gagctgaatg agcgattatt
     7381 tgacaatggc agaactagag aacaaaacgt taaaagaagt ttatagttat gccaaagaat
     7441 ttaaaattcc ttattacagt aagatgaata aaaaggaatt atctctagct gtgattcgtg
     7501 cacaagcaga gaaacaagga ttttattata tggaaggaat attggatatc gttggtcaag
     7561 atggttacgg ttttttacgg ccaatcaatt atggaccaag tgcagaagat atctatattt
     7621 ctgcctcaca aatcagacga ttcagtttga gaaatgggga caaagtggca ggaaaagccc
     7681 gtccacctaa agaatcggaa cgttattatg gtttgatgca cgtggaaagc gttaatggaa
     7741 aagatccgga agaagcaaaa gaacgtccgc attttccagc cttaacacct ctttatccgc
     7801 aaagacagct tcaactggaa acaacaccgc aaagactatc tacccgaatg atcgatatct
     7861 tttctccaat cggttttggt cagcgtggat tgatcgtcgc accgccaaaa gcagggaaaa
     7921 cttctgtatt aaaagaaatc gcaaatggta tcaccgaaaa ctatccggaa gtagaattaa
     7981 tcgttctact gattgatgaa cgaccggaag aagtaacgga tctagaacga agcgtgaaag
     8041 gcgacgtggt atcttctaca tttgatctgc agccgcaaaa ccatactcga gtttctgaat
     8101 tagtattgga acgcgcaatg cgtttagtcg aagataaacg agatgtcgtt atcttgatgg
     8161 atagtattac tagacttgct cgggcctata acttagtcgt tccaccaagc ggacgtacat
     8221 tgagtggcgg gattgaccca gccgcattgt ataaacctaa gaaatttttc ggggcagcaa
     8281 gaaacatcga agaaggtggc agtttgacga ttttagcgac cgctttagtc gatacaggaa
     8341 gccgtatgga tgacgtgatc tatgaagaat tcaaaggaac tggaaatcta gaattacaac
     8401 tttcacgtga attatctgaa cgtcggatct tcccagccat cgatatcaaa aaatcaggta
     8461 cccgaaaaga agaattattg atgccaagtg aagaattaga agaaatttgg aaacttcgaa
     8521 aaaatatgac tggagattca ttagaatata cggaacaact gatccgcttt ttgcgtaaaa
     8581 caaaaaacaa tcagcaattt ttcaaagaat ttcaagatgt ctcctttggt aaaaaaaatc
     8641 cgaccagaaa tctgaaaaga taattgcaac cttgactgaa acatgttaag atgttaatgt
     8701 tgtaaatcga caaatcaact ctggttcagc aaatggagca gggcaaagga gcgaaaaaat
     8761 catgaaagaa aacatccatc cagattacca cccagtagta ttcatggatt caacaactgg
     8821 tttcaaattc ttgtcaggtt caacaaaaac ttcacaagaa acagttgaat gggaagacgg
     8881 taatacatac ccagtcatcc gtgtcgaagt aacttctgac tcacatccat tctacactgg
     8941 acgtcaaaaa ttcacacaag cagacggacg tgtggaccgt ttcaacaaaa aatacggtct
     9001 caaagacgca aacgctgcgg aataataaaa acgctgataa ctcaacgttt agcagactat
     9061 ctgataggga tagtctgttt ttttatgttt tttgccaatt taaaacttcg tttgatgctg
     9121 aaaaagtcgt aggatcataa tgcacaaaac aatgaaaaac tagtatatag ttttaattat
     9181 ccacttgata aaaacacctt atattaacga aatgaagagg ggttatttcg aagtaaaaaa
     9241 ccatttacct aaaattagaa gctaagcttt cctttcaagg aaggcttttt gttttgttat
     9301 agacttgcga ttgtttgtga gctagatcgt tcattttaat gacttataca cgcggatgat
     9361 gggtcttttt cctctgttca ttcataatat tgaacggtac agctagcgat gctatactga
     9421 cctcagttcc aagaaaacgt taacattttt tgtgggggag aggtttttta tgaggtttga
     9481 tcaattgatc gcagaagaac tgattgtttt cgatccatcg atagattcaa aaggatcatt
     9541 atttgagcga ataggggatg tgttgaaaaa aacaaataag gtaagaaatg ttcaaaaagt
     9601 cgttaaggca ctattcaaac gagaggagga gatctccaca ggaatagaag atggatttgg
     9661 gattccccat gcaaaaagca aatatgtgac aaaaccagca ctgtgttttt ttcatacagc
     9721 caaattaacg gactatagcg ggatagatga aatgccaatt gaatatattt ttgccatcgt
     9781 agttcctgaa aaatcagcag atatgcatct agagattctc agcacactct ccagacgatt
     9841 gatgaatgca gattttagaa agggaataaa agaagccact acgccagcag atatcatcag
     9901 acagattagt caagaatgaa aatagtagag tctttgtccg agtatcgata gtgaagaaaa
     9961 aggaggaaaa taatcatgaa aatcgttggt gtaactgctt gcccgactgg aattgctcat
    10021 acttatatgt cggcagaaaa attagaagta acagcaagaa atctaaacca tcaagcaaaa
    10081 ttcgaaacac aaggtgtaaa aacagaaaat cagttatctg aacaagaaat caaagaggca
    10141 gatgcaatca ttcttgcagt agataaagaa atcgaattgg accgatttgc cgggaaaaaa
    10201 gtaaagcgtg tctctacaag ccgagcaatc aaagaaccgc aagtagtaat agacgaagca
    10261 ttgagagata ttggcgtatt cgttgtgtct aatgaaaaag aaccagctgc aaatgagaaa
    10321 aagccgacaa tctataatca tttcatgaat ggtgtgaatt acatgcttcc atttgtgatc
    10381 gcaggcggaa tcatcatcgc actgagtttt gcctttggga ttacagcggc tgatcccaac
    10441 tcagcagact ataatgtgtt agcagctgca ttttcaagga tcggaggaga tacagcgttt
    10501 gccatgatgg ttcctgcgtt aggcgcgggg attgctacat cgattgctgg aaaagcaggt
    10561 tttgccccag gaattgttgc cggattactg gcctctacag gtggttcagg ttttcttggc
    10621 ggaatgattg gtggcgtgct agcagggtat atctgtgatt ttctggcaaa taaaattagc
    10681 gtaaaaaaag aattttctgc tatttatcag ctgattgtcg tccccttttt atcgatctta
    10741 attattgggc ttttgatggt atttgtgatt gaacaaccga tcacatgggt gttggatgct
    10801 ttgacaaatt ggctgaatag cttgggaaat acatccgggc tattgttcgg attacttatc
    10861 gggatcatga tggcagctga tatgggtggg ccgatcaata aatcgacttc gactttctcg
    10921 atcggtttga tgtcagctgg agtaaatgca ccgattgctg cttgtatggc tgcaggaatg
    10981 acacctcccc taggtattgc tttagccact ttattattca aacataaatt taccaaagaa
    11041 gaacggactt caggtcaatc atgctggatt ttaggcgctt cgtatattac agaaggtgct
    11101 ataccgtttg cggtatctga cccgatccgc gtgataccta gtttgatgac aggttcagca
    11161 gtcgctgcag ggatttccat ggcagtagga gttacttcaa tggcacctca tggagggatt
    11221 tgggtgatgc taattccaaa tgttatgaac aatttaccta tgtatgtctt ggcaattgca
    11281 tcaggtacag tcactacagc ctttatgctt ggactattga aaaaaacagt gaaagagaaa
    11341 aaacaagaca agtggacgaa aaatgaaaat ctaacaaatg aggtggaagc gtaatgttat
    11401 atacaatgaa agagttattg aaagtggcta aagagaatca atttgcagta ccagccttca
    11461 atatttgcag ctatgatatg atgaaagcaa tcatcgaaga agcagagaga ttagaagcac
    11521 cagtaatctt agaaattcat cctgatgaaa tcgcctattt aagtgatgag tttgttgcgg
    11581 cagtaagagc ctatgcttat acaagtaaac tgccgatcgt tatccacttg gatcatggcg
    11641 gtacgataga agatgtctta cgggcgattc gaaacggcta ttcttccgtg atgatcgatg
    11701 cgtctttgaa atcattcgac gacaatgtac agattacgaa aaaagtggtt gcgttagctc
    11761 atgcggtaaa tgtctcagtg gaggctgagc ttggaacgat tgggaataat ggttctgctg
    11821 aaggaggtgc tgaaacaatc atttatacag atcctgatca agcgaagatt tttgtgaaag
    11881 aaacaggaat cgatacactt gctgtagcaa ttggaacagc gcatggcttg tatccgaaaa
    11941 acagaactcc aaaattaaat atcgacttac tgaaagaatt gaaccaacaa atcgatcttc
    12001 catttgtttt acatggcggt tcaggtaatc cggacgcaga agtcagcgaa gcagtaaaat
    12061 acggagttgc caaagtgaat ttgagctcag atcttaaaag cgtctttttc aatgctttga
    12121 gagaaatatt gagtgaaaat ccatcgatgt acgagccgaa tgcaatttat ccagcagcta
    12181 ataaaaaggt acaagaagtg gtcaaacata aaatggaaat tttgaacaca gtaggcaagg
    12241 ctagtttata ctaaaaataa aaatgtgtgg tcaagagggt atgacaatag tcttgtcata
    12301 ccctcttaac cacacacgat gttcagaagt agttttttcg aaaaatttct tcttatttct
    12361 cgaagccaat cacttctgtc acaacctctt ttttcctgtt actatataaa gtaaatgggg
    12421 tgattttatg ttgaatgaag tggagaatag acagcaggag attgtgaatt atctaaaaaa
    12481 caaccaattt gcaagagtca ccgatttgat cgagttagta aattatagcg aagccacgat
    12541 taaacgtgac cttgtttttc tggaaaaaag tggattgatc cgtagagttc gcggtggcgc
    12601 catgcttatt gacaatcgaa aaattgacgt gccttacctg atgaagatca ccaaattaga
    12661 agaagaaaca gaaaaacgta agattgcaga tacagcttct tctttgattc gagatgatat
    12721 ggtcttattc ttggattcca gtacaacctc tttacactta gtcagaaacc tagcaagatt
    12781 tgatggtttg cagatcatta cgaatggcat cgtcacagct gctatgttga gtgaatatac
    12841 aacggcaaaa gtatctgtat tgggaggggc aattgttccg aaacgagcaa caatcaacgg
    12901 ttcaaaagcc tataacgatg cattgtcata ttatgcggat ttagcgataa tgagttgtcg
    12961 cgggctagat ttcagccaag gagtaacaga aactcatgaa ggagaggcaa tggtcaaaaa
    13021 agcttttaga aaacaatcag accggcttgc cgttttagtt acacagcaaa aaattgcaca
    13081 taaattcatg catcaagcca ttgcttttca tgaaatcgat tatctggtga caagtaaaga
    13141 actgacaaaa gaacagtatg cgaaagtaaa ggaacaccat atcgaatgtc tgtacggata
    13201 aattattacg tgaatgttct aagtggcttc ctcattttta gataggacct tatctttgct
    13261 cgcttctctc agccgatggt ataataatga aaaggttatc ttgttagata atcttacttt
    13321 tttatccagg agaaaggagt gcagaaaatg aaacatgtga aggcgttggt agtgaaggca
    13381 atcatgatct gggcgattct gtgggtcgtg ctgacaggtt tatacggtgt tagctttatg
    13441 gattcaacga ttgttggggt aattattgta gtcatgatct atgtgctagg ggatctgttg
    13501 atcttacgta aagtagggaa catcgcagcg acgatcgcag atgctggttc agctgcagtt
    13561 atcttgtggc tctatctata ttttatgaat gatacagtcg atatttggat gaaagtattg
    13621 attcctgctc tattgatcgg tgtggcagaa tggttcttcc acaagtggtt gctaagtcaa
    13681 ggcatcgtac cagatgaacg gaaaatggaa taaatcaaca gttaaactat tcttatcgat
    13741 aaatgaaagc tttaaaaata aatagatgtt taattaagaa taatcataaa ctatttttac
    13801 tcaaaaaacc gctcagatta ttttcagatc tgaacggttt tttttatcta ttcatcaaga
    13861 tcaaatttca tttttcaatg attcaatcaa ttcgccctct gcttcatgac tatgttttcg
    13921 gccattaaca agatagagtg ctgtacatat caatggaatg actccggcaa tcacatacaa
    13981 tcctctaaat gaagtaagag attttagcat ccccatcaaa taaggaccaa cgcctaagcc
    14041 taggtctaaa ccaataaagt aggtagataa agcaatactg attcgatgtt cgcggacgat
    14101 ttttaaacaa acagcctgtc catttgacat aaacgtacca taacctaggc cgactaatgc
    14161 tccagagata agcaatgtca cactattcgt cgttacactc agtaaaaata atcctgctgt
    14221 taagaataaa tagctaggat acatcacgta ttgttctcct ttgacatcga aaatccttcc
    14281 agtcaatgga cgtgtaaatg tgatgaccaa agcatatacg acgaagaaaa atgacccagc
    14341 agcaactaag tggattactt ttgtataaga agataaaaat gataaaacac ttgaataaga
    14401 aagacccatc aaaaaggcaa ttcctgtaat gaaaagtgct ttcttttcaa taaaactatt
    14461 gatattccat gagcgaagag atattttttg ttcttcagat aatggaatat tttttacagg
    14521 aaagacaagg caggcaatcg taacaaagaa gaccagtaca attgaaaacc agataatgaa
    14581 acgaaaatca gtcatattca gcaaaatcat tcctaaaaat ggcccaacag cggcagctaa
    14641 actagtactt aatccataat aattgatgcc ttcccctttt ttagatgcag ggatatatgc
    14701 agtaacgata gcatttgtag ctgtagaaac tgtaccgtag ccgaatccat tcaagaatcg
    14761 aactaaatac aatgccccga tagttggcgt ataaagatat gctaatgttg aaaggagata
    14821 gaagatcgcg ccgcctctta aggtaaactt ccgaccaaat agttctaact gtttccccat
    14881 gaataagcgt gccagcaatg ttccgatgat atagatccca gaagcaaaac ctgcttggct
    14941 caagctggcg tgtaaggtgt cttgcgcaat gactgccatg atgaccatca acagataata
    15001 aatcaggtag acaataaaat tgatcaatgt gattccaata aaatatttat taaataattt
    15061 cttttccatg tatgttatac attcccctta agctgaatat gcttttaatt tttgtatttg
    15121 aacttctaca tcgtgataat tggatggata gcgatcaaca agttggcgaa taaataaatc
    15181 tcttgtagac ataaacagat ttaaaaattc ttcgcattcg tttgttggat tctcatccac
    15241 tgctttgatc aattcgatcg agttttgata cgcatcattt aaaaagacta aagaagcttt
    15301 catatcaaat cttgtttgat atgctgtttc taatcgatta atgattgtgt tcatccagta
    15361 attcatgttt tttccctcta ttcttaaaaa attttacttc tgcagtataa cgaataaaaa
    15421 aaaggaaaaa aatcgcataa gtgatggaaa aaggtactta acaaatgtta atatcattag
    15481 taaaatacct ttggacttta aaaagtttta aagtactgta tgatcttttt agatgaaagt
    15541 ttggatgggg gagcatcatt ggatagtaga gtcttaaagg aatatttgga taaaaatgat
    15601 tttccgatta tacgcaaagg atatcataaa tatttgacat tcgaagggat ggaagattcc
    15661 catacgtata tcttgaaaga gggaacggta aaagcaagtg ttatttcaaa tgatgggaga
    15721 gaatttaacc ttcgctatat caaagatctt gaaatcgtct ctcttttaaa agacgaatat
    15781 tcacaattca tcgattcgcc gtataatatt cgagttgaat cagaatatgc agagttttat
    15841 cgaatcaatc gtgtgcgctt ttggcaaaat atcaaccaag acccaaagct acaacactat
    15901 gtaaaagagt attaccgcta tcgtctgctt tattcgatga aaaaaatgca gcaaatgcta
    15961 ttaaacggaa agctgggagc tgtttgtacg caactttacg aattatatga tttattcggt
    16021 gtgcggacag aagaagggat gctgatcgat ttttcgatca cgaatgaaga aatcgccaaa
    16081 ttttgtggca tctcaactgc tagcagtgta aacagaattt taaaacaact gaaaaaagat
    16141 ggtattatca ccgttaaaga ccatcatatc gttattttga atatggactt gttagaagac
    16201 aatattatct tttgatgaac aaagaggttg tgaaatcagc gttttgacct gagtattaga
    16261 gaaacaaacg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
    16321 agctgattga acaccgttta ccaagaggtt gtgagcctgc cgtttaactc tgagtcacaa
    16381 ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg tgaccgagga
    16441 gttggtagga gaacaccgtt taccaagagg ttgtgaaaaa gctgttctgc atcaagtaat
    16501 aagaagagcc aagtaaaaac agtctcttat gtttttaact tggctggact tatcaccgta
    16561 gatgtaagct tttgaacgcc gtttatttgc aacaaaaggg gctgtgacac gatttttgtc
    16621 acagcccttt ttttgcgaac attcatttag caatttttag aaacttctgc cgccaccgcc
    16681 aaaagtcccg ccaccgctag aatgggtagt acttccgcca ccgccgcttc caggaggtgg
    16741 gcttttgggg attcttcttg tcgtgacaaa agaatttgtc agacgatcgg ttttttcagt
    16801 taatttgata ctggattttt ctcggaatgg atatttgtag atgcctgatt tcaattgata
    16861 acgcgaaaca gtcacaaaat aaaagacaag actaaggata agagccaaaa caacagcaat
    16921 gatgatttct actgtcgtca aaaccttata gcgtgtaatt ttgccagttt cttcgtctac
    16981 acgataatgt cctcctggaa ctccgtcttc cacatatgtt gctgatttag acaggtaagc
    17041 ttttgcagca ttgtaatagg cagcatttgt catctgatca taaacatcat ctaagatcga
    17101 atcgattctg ctatcagtca aataatcgat catattacca gaagtcgaaa tgtagatttc
    17161 acgctgtccc atatctaata gcaatactga gccattttga tcatttccga ctgcgtctct
    17221 taagtaatta tcagcaaagt ctcttggttc ttctgaatta gatgtagtcg ttacgatcaa
    17281 gactcgtcct ttgatttttt cattcagctt ttctgcctgt tgttccaaag attggatttg
    17341 atctgttgtg aataacccag cctcatcatt taccgtagct gtatctgcat gagtagccac
    17401 cggccagcca acacttacta gcaaaagaaa gaataaaaaa aactttttca tcatatcaag
    17461 taacctccga tcagaaagag gatcgctagc ccagcgaaaa tcccaagagc ggtcagtccc
    17521 aattttttct ggctgatcgg caggacaccg ctgacctttc ctgtttgtcc attcattgcg
    17581 tagtagtaga cttttttact tgagtcattg cttcgataag taacaagcca taccggcagt
    17641 aatacgtaat tatttacttc gtttgtgatt gaagcagaag ttcgcacacc ggtcaatgtt
    17701 gtatatccat ttgcggtatc ttttaacagc ttctctgaat agtcttttaa ttcagattga
    17761 atctgtgatt tgattgcctc atattcgata tctctttttt ctgcttggaa cccggctaaa
    17821 tactggcttt taaaatcaat tgctttatct aacgggaaag gttgcacgcc ttcgaccatc
    17881 ttttgttgcg tattttttga taaggcattt tttactagtt ctttaaaaga aagcttgcct
    17941 gctcttgaaa cagcaaattg cttcgtttct gtgtattcga tgtcacctat ccgccatatg
    18001 cggataactg taccattcgc ctgcatggca ccatctactt cagcatctgt agcccaataa
    18061 gggaaataca cacctgtcaa cttatcgatt tgatccttat tgaaaaaagc tttgggaaca
    18121 aaccattttt tcttggtcca agcgagaaat ctgttgattg cttcttcttt ctcgacagca
    18181 aaaggaagta ctttatttgg caagaattct ccgcttaaac gtcccgatag tacgacggga
    18241 ttatggcagt agtaacaata agtcgcagca gttgtcgcat cagtcacgat ctctgcacca
    18301 cagttgggac aaagaaaaag atctatcgct gcggtattat ctgcagattc gtccgctgta
    18361 tctgaaaaac cgcctgcctc gtcaaccgct ttgcgctcgg cttcattcat atcggcaagc
    18421 tgttcttggg ctgtaaaagt aaaatcttcg gtaggtgaag gttcttcttt gctttcttcg
    18481 ccagccatac gcgcttcttt ttgcttttgc tcatattggc tgacttcttc ttctgtatag
    18541 atattcaggc agtattcgca atgaaatttt tggtctttgg gatcaaaagt caacggaccg
    18601 ccgcaattag gacatttatg tgtaagaaca tccatcagct gcacaccttt ctaaataaat
    18661 taattaacgg ggattccttt aaacggttta ccacagtttg gacagaattt agggatgccg
    18721 ttggaaagat ccacgatctc attgcattca ctgcatcgca tttgtagctt aggctgaacg
    18781 ttttctacag gtttggcagc tccgcagttg gaacagaatt ttccggaatt ttctgtcccg
    18841 caaactggac atgtccaagt atccgcattt ccttgtgcag tttgttgatt ttgtttttct
    18901 gcttgttgtt tgatttgttc ttggttattt tgagctgctt gagaaagata agaaccattt
    18961 gcattcattc ccatacctac acccataaag ccggtcattg ctcctgcttc attttttccg
    19021 gctgcttcca taccacgtgc aatggaacct tgcacgtatc cttcacgtac gctaggatca
    19081 cctagcatcg ccccttcgtt acgcatattg atcagtttca cagaatcatc ggtgtaagaa
    19141 atgctggcta cagccacaga aacaatctcc atcccacgta attcacgcca agaatcatcc
    19201 aatgcagtat ccatgtattt gcttaattcc aaactcttag atggtacata agaaatccgt
    19261 tgaccatctg cagacatctg attgattgct gattgtagcg ctgttaggaa ttcagctaaa
    19321 tactgctcat tgatatcatt gatttccact tgtgttttgt ttttagggat tgcgtttgta
    19381 taaaataaaa tcgggtcaac gacatgaata gaataagtgc catgtgcccg taagaataat
    19441 tctgcgttat agaaattgtc aaagtagttc aacggcgcgc ttgtaccgaa tttgattccc
    19501 tttatttctt ggagattgat ataaaaaact tgttgtttct gcggtgtgac gccgccaaat
    19561 ttgaatcggt caaaagtttc agcaattgct tctttcagtg ttccattgaa catggaagga
    19621 gctgcgtcat ttttaatggt gtaataacct tcctcagcgg tataatcgat gatttttcca
    19681 ccatcgacca aaagcatcat catgtttgga tagacatgga cgactgttcc gtctgttaaa
    19741 acatcttctg ttcctttacg attggagcca cgtttgtcat ctttgcggac tttgacacct
    19801 tttgtcatga ccgttgtatc actcatattg tctggttcga tgacttctag ccattgatct
    19861 gccagaccgc cgccaaccat acttgttgct gctttgataa gacccataaa aaattcctcc
    19921 tctgattcag ctagtgccaa acaattattg tttatttatt ataccatagg aagatgtgaa
    19981 acttctcgga ctagagacgg aattgaaatg gaaaattgcg tattgtttaa atacctgaaa
    20041 aagccgagaa ggatagtagt ttttttacct aatatcacgt ataataagag atagtgaatt
    20101 ttttaaaaaa gggggatcct tatggcatat caagcacttt atcgagtctg gcgttcgcag
    20161 cgtttcgaag acatcgttgg acaaaaagct gtgacacaga cactaaaaaa tgctattgtt
    20221 tcccacaaaa cctcacatgc ctacttgttt acaggaccca gaggaaccgg aaagacgagt
    20281 gcagcaaaga ttttcgcaaa agcaatcaac tgtccgaaca gtaaagacgg tgagccatgc
    20341 aacgaatgcg aaatgtgtcg atcaattact gctggtacgc aagaagatgt gatcgaaatc
    20401 gatgctgcca gcaataacgg agtcgaagaa atccgattta tccgtgatcg ggcaaattat
    20461 gcgcctacaa aggcagcgta caaagtgtac attattgatg aggtacatat gctttcaaca
    20521 ggagctttca atgccttgtt gaaaacattg gaagagccaa aagaaagtgt catattcatc
    20581 ttagcaacga ccgaacctca caaaatacca gctacgatca tttcacggac tcagcgattt
    20641 gattttaaac ggatcaatac ggcagatatt gtcgaacatc ttgaatttat cttgaaagaa
    20701 tcgactatca gctatgaacc agctgcattg aatgtgattg cccgttctgc agaaggcggg
    20761 atgcgggatg cattgagtat tcttgatcag gcaatctcgt ttagtgaagg aacaattact
    20821 ttagatgatg ccatgcaagt gacaggtagc ttaactgacg aaatgatgga cagttacttg
    20881 agtgcttgcg tgaaaaaaga agtaccagaa gcattggaaa cattgaacca aattctcgca
    20941 gccggcaaag aatcacgtcg ctttctagag gatatgttac aatactgccg tgacttgctg
    21001 atgtatcagc aagcacctaa cctagttgag gaacaatcga gtacgatcag aagtacattt
    21061 acagaattgg cacaaactgt ttcagcggat cagctgtttt caatgataaa gattttgagc
    21121 gatactcaaa atgaaattcg ttttaccaat agtgccgcta tttatttgga agtcgctaca
    21181 gtcaaacttg cacagctacc gtctgcttct gttccttcag cagatgtagc atttgcagat
    21241 gatcagatag taaaacagct acaacaagag attaaggaac taagaaacga aatcaaggaa
    21301 ctacgcagta atacaggaat tgagaataca aaagtacagc cggtacagca aacaaatcat
    21361 aaaaaacaga caagtaccta tcgtgtaccg acggaacgag tatataaagt tctgcgggaa
    21421 gcgacaagac agcatctgaa ccaagtaaaa gaagtatggg aagacttgct gatgagtcta
    21481 tctgtcactc agcgagcaat gcttaaagca agcgaaccag ttgcagctgg acctacagga
    21541 ctagtgattg cgtttgatta tgagattgtt tgtcaaagag cagcaaatga cgaagaattg
    21601 cagctttcgg tacacaacaa cttaagccga atgatcaaag attatgcgcc tgatcctgtt
    21661 tatattacaa gagaaagctg gccagaactt cgaagaaatt atttgacgca ggcacaagaa
    21721 gagggaacag aaaactcttt tgcttctgaa gactcagaag gagaaatcga gttgcttcct
    21781 caagaacaag aagtaccaga agaaacactt gtagtaaaag cagaggaact gtttggctcc
    21841 gaagctgtta cagtggtaga tgattaataa aaaggagtag ataattatga tgcgtggaat
    21901 gggcaatatg caaggcatga tgaaacaagt acaaaaaatg caaaaagaaa tggcagaagc
    21961 acaagagctg ctaaataaaa cagagtttac aggtaaagcc accaatgaat tggtcaaagc
    22021 ggtttttaca ggcgatcgtc gcatgaaaga tatccaaatc aatgaagaag tcgttgatcc
    22081 tgatgatatc gagatgcttc aggatcttgt cgtaatggca gtcaatgatg cgatcgcaca
    22141 aattgatgtg gaaactgaga aaaaattagg gaaatacgca aaaggtttgc caggatttta
    22201 gttttaaact gaaggagtaa tgaatatgca atatccagaa ccaattgcga aactgatcga
    22261 cagctacatg aaacttccag gaatcggtca gaaaacagcg acaagactcg ccttttatac
    22321 gatcgatatg aaagatgaag tagtaaatga atttgctaaa tctttgttaa gtgtaaaacg
    22381 cgatttgcat ttttgtagta tctgcggaaa catcacagaa gaagatccct gcgaaatatg
    22441 tagaaatcct tcacgtgatc gaagcgttat cctagtggtc gaagagccga aagatgtaat
    22501 ggcaatggaa aaaatgcgag aatatcacgg actttatcat gttctccatg gtgtgttatc
    22561 accgatggaa gggacaggtc cagaagacat caatatttct tctctgttgc agcggctgca
    22621 tgatgcagat gtaaaagaag tgattatcgc aacaaatgca acaacagagg gagaagcaac
    22681 agctatgtat ctctcacgtc tgatcaaacc cgcaggtatc aaagtgactc gtctagcaca
    22741 tggtctgtct gtaggctctg atatcgagta tgcagatgaa gttactttat tgaaagctgt
    22801 cgaaggaaga cgtgagttat aaatttctaa ttaagtgttt tttcagatac aatataaaag
    22861 cagcacaaga gatgggggca acaatgtgaa cggactattt attacaatcg aggggcctga
    22921 tggtgcagga aaaaccagca tcttgaatga actttaccct cttttgaaag aagtagcaaa
    22981 aacagaaatc attcaaacaa gagaaccagg aggcattccg attgctgaag agatccgagc
    23041 agtgatcctt gatccaaaaa atgatcggat ggatgaacgt acggaagcac tgctttatgc
    23101 agcagcaaga agacaacatc tagtcgaaaa ggttctgcct gctctggcaa aagggaaaat
    23161 cgttttatgc gatagatttg tcgacagttc tcttgcttat caaggtgcag gccgacgcat
    23221 cggcgttacc gagattgctc ggttgaatga gtttgctaca gaaggtacta cgccggattt
    23281 taccctttat cttgacgtag attcagacac cggattaaga agaatcaaga aaaacagaca
    23341 aaatcaaatc gaccgtttag attcggaagg tttggaattc caccagcgtg tacgtcatgc
    23401 ttacttaaag ctggcagaag agaatccgga aagaatccac aaagttgatg caagaaaaag
    23461 ctttgaagaa gtgctgcaaa caagttatca tacaattatt gaacagtatc cgcaattttt
    23521 tgaaaactag gaaggtgaac gtttatgaaa attattttag cgattatcca agacaaagac
    23581 agcaaccgtt tatcaaatga attgattgat gcaaatatcc gtgccacaaa actatcatcg
    23641 acaggcggct tccttaaggc tggaaacagt acgtttatcg tgggtattga agatgaccgt
    23701 gtagaagaag cattggaaat catcaaaaaa acgtgtgaat caagaaagca atttgtatct
    23761 acacctgtta cactggatat ttcgatggat gggggcgtcc cttatccagt agaagtagaa
    23821 gtaggcggtg ccacagtctt cgtcttacct gttgaaggat tccatcaatt ttaggaggag
    23881 ctgagctatg aatcaagaaa taaacttggc tcaaatgcaa ccgatcgtgt atcagcagct
    23941 tcaacggagt tttgagcatg ggcgtatcgc tcatgcttat ctttttgaag gggagaaagg
    24001 aacaggaaaa catgaagtag gtatatggct tgcacagcac ctgttctgta cccaaatgaa
    24061 agacaatctg ccttgtggaa aatgcaacaa ttgtcagcgg atcgaaaaga aagaacatcc
    24121 agatgttttg gttattgaac cggaaggaca aaccatcaaa gtcgatcaga tcagacgttt
    24181 gcagacggaa ttcagtcgca gcggttacga atcaagaaaa aaagtttttt tgatcaaaga
    24241 agctgaaaaa atgaatagca gtgcggcaaa cagtttgttg aaattcttgg aagagccccc
    24301 tggagatttt ttagcggtgt tagaaacaga tgcagtagga agaattctcc ctactattca
    24361 atctcgttgt caagttctgc attttcaaga actttcgaaa gaagcactga tccatcgatt
    24421 gcaagaagag cagattccat tggaaaaagc aaaacttttg gctttcttaa caaacagttt
    24481 aggaaaagca gttgaaatct ctcaagatga atggtttaat gatgctaagg acttgattta
    24541 tcaatggttc gtttacttgc aaaagcaaga tactcaagcc tttatctatg tccaaaaaaa
    24601 attggtaaaa tcatttaaag aaaaaacaca acagtttaca ggattatctg tcttgatgtt
    24661 ttattatcag gaagcacagc gtcaagcagt agcaaacggc gaatatggca aagtgaaaca
    24721 gataaaccag ataattgaac ggatattgat cgcagagcag aaactacgca gcaatgtcag
    24781 ttttcaggct gttgcggaac agttcgtttt gcatacgatt ttttagcttg ccgcaaagga
    24841 tgatgatcaa ggaggatgga aatggtagaa gtagtaggtg tccgatatag agatgcaggt
    24901 catatctatt attttgcacc aggagactct gaatatgttt atagtgacaa ggtattagtc
    24961 gaatctcagc aatccttaca tattgctact gttgtccttc ctaaaaaaga gattgatccc
    25021 aatgaccttc ctgaagctat ccgtccgatc ttgcataaag cgtcacaagc tgaattagaa
    25081 aaagacaaaa aaaacaaaaa agatgcttat gatgcattga aaatcgcaaa agaaaaagtg
    25141 cagttgcgtg agttggaaat gaaaatggtc aaagccgaat acacatttga tcgaaataaa
    25201 ttgatttttt ctttcacagc ggacgggcga attgacttta gagaattggt aaaagatctg
    25261 gccgctgtat ttcgtactcg tattgagctt cgacaaattg gtgtccgcga tgaagcaaag
    25321 ataataggcg gaattggtcc atgtgggcgt ccattatgct gttcttcttt tttaggcgat
    25381 ttcgtgcctg tatcaatcaa aatggcaaaa gatcaaagtt tatctttgaa tccaacaaaa
    25441 atttccggtt tatgcggacg cttgatgtgc tgtctgcaat atgagagcga agaatacgag
    25501 acagcgaaaa aagaactccc tgactttgga aaagtaattg ctacacctga tggtaaaggg
    25561 aaagtcgtag ggttgaatct gcttagccgt atcgttagta ttcggctgac tggacgggaa
    25621 aatcctgtcg aatatgaatg ggatgaattg aaagaattta tccaagtagg tgagatgaat
    25681 ggataagaga tcattatacg acggattgaa tcaattagaa tcagagttga gaaatacatt
    25741 aacagaactt gttgaaatga agcatgcttt gcatgagatc gtagaaaaaa atacaacttt
    25801 ggagatggaa aatcaacgtc tgcgggaaca cctgcgggaa ttgaaccaag ttgcacaagc
    25861 tccagctgaa aatgtgaagc aggaactttc taagtctcgt atgaatcttg aaaaaattta
    25921 tgaagaagga ttccatgtct gctacgattt atatggatca agaagggaca atgacgagcc
    25981 ttgtgcgttt tgtctagaag tgatctatcg cgaaagcggt cgatgaccaa taacaagagg
    26041 agactggagc ggagaaatac tgctccagtc tttttattaa ggagattgtg acagatgtat
    26101 aatcaaaaaa gtttcaaggg aaatccggta tatggggatt tatatcttgt accgacgcca
    26161 attggaaatt tagaagatat gagctacaga agtgttcgta tgctgcaaga atccgatctt
    26221 atcgcaagtg aggatacgcg taatacacaa aagttattga atcattttga aatccataca
    26281 ccccaaaaaa gtttacatga acataattat aaagaacgtg tccctcaatt gattgctttg
    26341 ttaaaagaag gaaaaacgat tgctcaagtc agtgacgcag gaatgccttc catcagcgat
    26401 cctggacatg agctagtttt agcctgcata aaagaaggga tttctgtgat ctctatccca
    26461 ggacctacag ctgggatgac ggctttgatt gcttcagggc tagtgcctca accattttta
    26521 ttttatggct tccttccacg taagaaaaaa gaacagaaag aaacattgaa aaagctaaaa
    26581 gaacagacag ttacgctgat tttttatgag tcaccttatc ggatcactgc tacattgacg
    26641 aattttttag aaatttttgg aaatcgccaa gtcgttttgt gtcgggaatt gacaaaggtc
    26701 catgaagaat accttagagg aagtacggaa gagctactcg cttatatcga agaacatcca
    26761 gtaaagggtg aatgttgtct gcttgtagaa gggaatttgt tcgaaatgcc agtgacggaa
    26821 gttgatgaag agttgggaac gttaaaggaa caagtagaag caaagatcgc ttcaggcgaa
    26881 aaaccaaatg ctgctatcaa agcagtagcg ctgaaaaatg ggctgaaaaa acaagaagtc
    26941 taccgtcagt accatgaatt agactgagat agtataagtt gtctataatg aaagactaat
    27001 gaggaaatca gctactaact tttcaaatct cttgaacgcg aacgaatgtt cttgtataat
    27061 gaataagagg tgaggatgat ggagctgcaa tttcctttac ataatgatac atcaagaaaa
    27121 atcattcata ttgatatgga tgcttttttc gcttcggttg aagaacgaga tcatcctgag
    27181 tttcgcggac atccattagt aattgccaga caccccagtg acactggtgg gcgtggagta
    27241 gtgactacag caaattatga agcaagaaaa ttcggaattc attcagcaat gagtgcacaa
    27301 aaagcgtatg agctttgtcc gcaagccatc tttaagcctg gcgaccatca taaatacagc
    27361 gatatttcca aacaagtacg ggaagtattc tatcgttata cagatattgt ggaaccagtt
    27421 agtatcgatg aagcgtatct tgatgtgacc gaaaataaaa tcaattgcaa gtcagccatc
    27481 aaaatcgccc gaatgatcca agcagatatt tgggagagtg tccagctaac atgttctgcg
    27541 ggagtaagtt acaataagtt tttggccaaa ttagcatcag actatcaaaa acctagagga
    27601 atcacggtag ttgcccctca agacgctgtg ccatttttaa aagctttgcc aattgaaaaa
    27661 tttcatgggg tagggaagaa aactgtacct agaatgcatg agctagggat ttatacagga
    27721 aaagacctat atgaatgcac agaaatgatg ttgattcgaa attttggaaa aatgggctat
    27781 tctctttatc gtaaagtcag aggaatccat gattctccag taaacgttac aagagagcga
    27841 aaatctgtgg gaaaagagca tacttatggg cagccgttgc aaacggaaga agcagtgctg
    27901 actcagttgc gccaactagc tgaaaaagta gaagaagcac tgagaagagt ccaaaaacat
    27961 gggaaaacag tagttttgaa ggtcaggtac acggactatt ctactgtgac caaaagagtg
    28021 acattgccag aatatatcta taaaaaagaa gcactctttt atcaagcaag ccttatatgg
    28081 gaggaaatct tgggagtaga aaaaggaatc cgattgttag ggattacttt gacgaatctg
    28141 gacccgatga cttatgaaaa catcgtcctt ccattatggg aaaatcaaga aatataaaaa
    28201 cagctttgag agttgaaacc ggaaatccgt tttctctccc aaagctgttt ttatatcaag
    28261 tcttgctttt tgatttgttc ataggtttct gttgcatccg tacatttttc ccaaatcaca
    28321 acttgattgg attctaaaga tttttggacg tcaatgatcc gctggttgct acttccgcga
    28381 aattgtagtt ttaggtcacg tttgcttaat tcaaagcgtc cgtcaacaag gatatcgatt
    28441 tgcgaaagga gttctaattt atcttctgaa tcttgtagca attcatcaaa ggcatagcct
    28501 gtccatgacc agatatcttt tgtcgtaccg aactcttttc ggatacgtcg tacgacttgc
    28561 agacaaacat ctgtattcaa aaaaggttcg ccaccaagta aagttaatcc ttgcacgtaa
    28621 tcgttgcgca agtcagcgat gatttgttct tctaattcag gtgtgaatgg tttgccatat
    28681 cggaaatttt gtgcagcttt gttgaagcag ccttcgcagg caaataagca gccactaacg
    28741 taaagactgt tgcggacacc ttcaccatcg acaaaattaa acggtttgta gtcagcaata
    28801 tagttttggc tgtagtcttt tgctaaccat tcttttggtt ttggatttct cataaaaaaa
    28861 cttctttcat ttaaggttca aagatatagc tattgaagat aaaatctact cgaccacata
    28921 aataagaaca aactgaccta aatccattgt tcaagaaagc ttgttcttat tttgatagag
    28981 ggcgttcaaa gatacaaagc catcgaaaga gggtggtgaa aaaagacttg tcatagcctc
    29041 ttattctgaa taaacggtgt tcaaaagctt gcatctgcag taataagccc aactaaacga
    29101 aaaatatgag gaactatttt tgtttagcta ttcttattac ttgatgcagg acagcttttt
    29161 cacaacctca tttcatgtgt ttcacacgtg aggaaatttc tttatggcgg ccatgtacca
    29221 tcggacgagc ttgcggattt cctaaatagc cacaagtacg tttgactaca tcacatgttt
    29281 ttggatcatg atttccacat tctggacatt cgaaccctcg ttctgtcggt ttgaaatcac
    29341 cttcgaaacc acattgatag caatgatcga ttggcgtatt cgtaccaaga tacccgattt
    29401 tgtcatatgc aaaatcccat acagcttcta aagctttcgg attttgttgg agcatagggt
    29461 attcacagta gtggatgaat ccacctgaac aatattttgg ataatccttc tcaaattcca
    29521 atttttcaaa tggcgttggg tttttacgca catcatagtg gaaactgttt gtataatatt
    29581 ctttatctgt gatattttcg atgatgccga atttttctgt atccaaacgg cagaaacggt
    29641 cagttagact ttcacttgga gttgaataga cactgaaatg ataaccatat tcatcccccc
    29701 atgcatctgc attagctttt aattctttga cgatatcaag tgtgaaagct tttgcttctg
    29761 ggtttgtttc ccattcgcca ccgaagaaag ctgaagcaac ttcatacaat ccaatgtagc
    29821 ctaacgatac agtagctcga cggtttttga ataattcatt gacagaatcg gtacgtgaaa
    29881 gacgtttccc gaaagaacca tacatatata aaattggcgc atttgctgga atggcttctt
    29941 tacaacgttc tacacgatag agaagcgcat ctttcatgat ttctaagcgg tcagacagta
    30001 actgccagaa tttattctga tctccttttg attcgatcgc gattcttggc aaattcaatg
    30061 taacaacacc taaattcatt cgtccgacat ttacttcttg accattttcg tctttccacc
    30121 cttgcaagaa tgaacgacag cccataggta ctttgaaact tccagttaat tcaacgattt
    30181 tatcgtaatt cagaatatca ggatacattc ttttcgttgc acattccaat gctagttgtt
    30241 tgatgtcgta attaggatct tcagggttta agttcacacc ttttttcaaa gtgaagatca
    30301 gtttagggaa gatagctgtc cgcttttcac tccctaatcc attgattcga atctgtaaaa
    30361 ttgctttttg aatttctctt tcaaaccagt tttctcctag tccaaatcct aatgaagtga
    30421 aaggagtttg tccatttgaa gtaaataagg tattgatttc atattccaaa ctttgcatag
    30481 catcgaagat atcttttttg gtttttgctt ttgcatattc tttttggcgt tctggcgaat
    30541 cgatccattc ttccgcgtct tttagatgtt ttttatagtt caattctgca aaaggagcaa
    30601 gcaattcatc tgttcggtcg gcagaacagc caccatattg acttgacgct acattggcaa
    30661 taatctgtga gatttgtgct gtggcggtct ggatagattt tggcgattcg acttcggcat
    30721 tgccgatttt gaacccattg ttcagcatgc ctttgaaatc aatcaagcag caattcgtca
    30781 tcggtgtata aggatgataa tccaaatcat ggtaatggat atcgcctttt tgatgcgcat
    30841 ttgctatatg tggtggcaac attttcagtc cgattgattt tccaacgatc ccggctgtca
    30901 gatctcgctg tgtattgaaa acattactgt ctttatttgc gttttcgttc acgacagttt
    30961 ggtccttgtt gattagcttt tcaatcgaaa agttgatatc tgttgcttta ctgcgagcaa
    31021 aatctcgttt ggtacgataa ttgatgtact cttctgctaa ttcatattcg tttttttcta
    31081 gtaatatatg ttccacgatg ttttgaatct catagatttt gacattttca gcaaaccggc
    31141 ttttgatttc ttggacaacc aagtctgcaa tcatttgatt tttttcataa gtaagtggtg
    31201 ataatgagcc gtgaattttc ttttctgctt tgatcagtgc ttcattgatc ttttgttcat
    31261 cgaaatcaac atggcggcca tcgcgtttga tgactttcat ctgggatgtc tctggctgct
    31321 gagtaacgac agttttttct tcataattta ctttcattat cttcaactcc tgctaaattg
    31381 ttcttgtcta tcataaaaca aaatcgggga aggagcaact aaatatcggc tatatatagt
    31441 attcaatgtg ctgtataatt ggatttctat actatatgta gtgtatattt tattgtgaaa
    31501 aaaggtgaaa acttgtagct ggtggatttg ttttttgcat atttaatttt gctgaataaa
    31561 ataattgaat aaaatttttt attcagtcca agtaaaagtg acaaaaacat aagagcaaat
    31621 tattcgtaga tatgctacta tgcaaataaa tagaaatgaa aaatgaaaga ggaggagaag
    31681 aattggaaaa gatgatcgaa gtgaaacatc taagtaaaag ctacggcagc aaactttctc
    31741 ccgtcaaagc cttagataat atctcattca cagtagaaga aggcgagttt gttgggatta
    31801 tgggcccaag tggtgcagga aagacaacgc tgatgaatat cttgtccacg atcaatttgc
    31861 ctacgatggg cagtgtctcg atccaaggaa aagaaatcac aaaaatgaaa aacagtgagc
    31921 tcagtgactt tcgtcgaaaa aaactaggat tcatttttca agagttcaat ttgatcgata
    31981 ccctaaatgc aaaagacaat atcctattgc ccctagcagt tgaacggatg actaaagagg
    32041 aaatggaaaa gagagtaaga catgtagctc aattgttgaa tatagaagaa ctgctcaaac
    32101 ggtatcccga tgaactttct gtcggacaga gacaacgaat tgcagctgct agagcacttg
    32161 ttgttcagcc acaagtgatt tttgcagatg aaccaactgg ctctttggac tcgaagtcgg
    32221 ctacagaatt gttgaactac ttgaagacca tgaatcaaaa agaaaaagca acgatactgt
    32281 tagtcaccca tgatccatat acagcaagct actgcgatag aattttattt attaaagatg
    32341 gtgtgatttt ttctgaagtt gtcagaagag ggacaagaag agagttcttt gagaaagtaa
    32401 tagacatgca ggcgacgatt ggcggaggag gaaaaatgaa tgctgtataa actagccgtt
    32461 aaaagttttt ttcggcagtc acgaggctat cttgtttatt ttttcagttt gactttatcc
    32521 gtaatgatct attattcctt cagcgcaatg acctacgatc aatctttagt cagaagagca
    32581 agtcaagata cttcgataga tggtggattg agttttggtg gatttatcat tactgttgtt
    32641 ttgctttttt tcgtattcag tgccaaccga tttttcttga atagaaggca aaaggaaata
    32701 ggcatctatc agctttttgg tatgaataaa ctgcagatat caggtatata tgtgttagaa
    32761 atcatgataa ttggattgtt cgcctgtatt tctgggatat tactcggtat tattttttcc
    32821 aaactctttt caatgatttt ggttcgaatg atggatatgg atcttactag tccatttttt
    32881 atctccattc catcagtagt agatacttta tttgcttttt tcatcatcct tttagcagta
    32941 tctgtatatt caatatggaa aatatggcgc tatccaatga ttcgaagttt tggagaaaaa
    33001 gaacaagcag atagttcaac aatgcgcttc aggacaaggc accgattact cgcagtcatc
    33061 gggttactac ttttaacgag cggatatttt ggagctagtc attttaggga aatcacttca
    33121 tggatgatca gttatcatta tcttgataca ttagttttct tttttccttt tttaattttt
    33181 ttcgtttgtg tggtcggtac ttatttgttt ttctgttata catttcggtt atttttgatc
    33241 atattgagta aatggaagat ttattactat ggtatcaatt ttttgataat gggtaacacg
    33301 caagttcatt tgctcaaaag ttggcgtaca aattctctga ttacagtcat tctcggaatt
    33361 tctttaatga caattggcgg cacggctagt atctctacta ttttatccta ccaggagaaa
    33421 gtttcctccc cgatggacta tcagtttgat gttgagacag agaaaaaaat caagccgatt
    33481 ctagcagagg aagggcagaa gataactaaa gaaatcacct tgcattataa agtcattggg
    33541 agtatctata gtcaaaatct cgaagaacct atgtttcaaa ctgctaatct gatcacggaa
    33601 acacaatatc aagccttcag aaaagttaat ccagatttgc cgaacatttc tttgaaatca
    33661 cctggtcata ctgttttgct tgatcaaatg caaactcttt ttcggaatat ttcattatat
    33721 ggatctagta ttggactgcc agataatcaa gaattgaaga ttcaacaaat gcttccaagt
    33781 gtattaggtg acgaaacaat ggtatatcta agtcctgtac tgattgtaga tgataccgtt
    33841 tttgaaaaag caaaaggtgt ggattatcaa gtagtcaatg tagatgtatc aggtggaaat
    33901 aatgaaaaaa tagatgaacg actgagcaat gacctgtcga taaagtggct caatgctgtt
    33961 tattattctt atgatatcgt aaatggaagt ctacaaggag aaattagcac aaagaaactt
    34021 ccggatgacc aaatgaagga agcacaattt agccaagaaa gctctcgttt gaatttctct
    34081 tccagctatt cgaaattgcg tgcagttaat cggcgaatcg gtatcaatat ttttgtcaca
    34141 ctatttgtcg ggatgatcgt gattattgct actggtagta ttttgacagt ccggcaatta
    34201 gcagaagcag aggaagaaaa agaaaattat cacctattga ctaagctggg gattccacaa
    34261 agaaggatca gacggatgat cttcgagcaa aacgcactta ccttctttcc acccatgata
    34321 ttagggatca ctcacgcagt atttgcaatc aatgtcttca ctcagtatat aaaaactgcc
    34381 gattactggt tggcttatct tgtgagtggc ttattgatcc ttatctatct tttgctttat
    34441 gtcattacga gtcagttata ttgtcggatt atcgaggaat gatatacgga ataagacaag
    34501 ataataaata gaactaaatt ttgaatacca tttatttgga ataagaggtt gtgacacgat
    34561 ttttgtcaca acctctttat tttttatatt ttttaaaagt acgagaaatt ataaagaaaa
    34621 aagcagtgat ttgatgtgct atttttataa aagctatgtg acaaaactgt tagtaaaaaa
    34681 acggtttctg taagctagac acatgtttct ttttgtcaaa attagttaca ctttgtgtga
    34741 ggttatatga ggagggaatc caatgaacaa atggcgttct aatccaaaat tgaattattg
    34801 gggaaaattc atcattcgaa caatttttta tagtgcagta ctgctgttcc tgatttatct
    34861 gtatcactat aaaaatattc aaggaggaac ttttatctat aacgaatttt aggaggaaga
    34921 gtatggaaat caaaacgatt attgaagcaa tcgatgattg gggaataaat gaacctgatc
    34981 gagttgctta tcaggcggat gagacgcata cttttggtga attgaaacaa gcatcagacg
    35041 cacttgctta ttatttagaa aaaaaggtag aaggcgatgg accaattgtc gtttttggca
    35101 atttggagtt tgaaatgatt gtctcatttc taggggttgt caaatctggc catgcctatt
    35161 tgccgattga agagcataca cccaaggagc gtatcctatc gatctttcgg gtggctaaac
    35221 catctatggt catcagtatc ggggactgga tagaagatat tccttcggtt cctgtcataa
    35281 caaaaaaaga atttcaagaa atcaagaaga taccagtagg attttatccc agaaatagcg
    35341 taaaaggtac agaaaatttt tatattattt ttacttctgg tactactgga gaaccaaaag
    35401 gcgtacaaat cagtcatgat aatctagtca gctttgttca atggacgatg gaagactttg
    35461 ggatcaagcc tggtatgcat ttcttagccc aagcgccatt ttcttttgat ttatcggtat
    35521 tttctattta tcctgcatta gtttctggcg gaatgctgaa gccgttaaat aaagcagttg
    35581 ttcaagactt tcgtcaatta ttcgcgacat tgccagcctt gaagttgaat gtgtgggttt
    35641 ctactccatc atttatggat atttgcttga tggaacctac attcaatgga gaaaatgtgc
    35701 cggaattgga catgttttta ttttgcggag aagaattgac gaaaaaaact gcagaaagtc
    35761 tacttgatcg attcccaaat gcacggatat ataatactta cggtccaaca gaagcaactg
    35821 tagctatttc aagtattcag attgaccagc atgtactaga tagttatgac cgactaccga
    35881 ttggctacgt aaaaaaagac acgaaagttt ctattgtcca agaaggtaaa cctgtttcaa
    35941 aaggagaaac gggtgaaatc attattgcag gaccaagcgt atcaaaaggt tacctgaaca
    36001 atcccgaaaa gacagctgct gcgttctttt gtcaaaatga cttagcaagt taccatacag
    36061 gggatgcagg tagactagat gaatatgggc ttttatttta tgaaggacgt atggatcagc
    36121 aggtcaaatt acatggatac cggattgaac tcggggatat cgaacattat ctgctccaag
    36181 acaaccgaat caaacaagct gtcgtcgtac caaaatacca aggaacaaaa gtacagcagc
    36241 tggttgcttt tgtcgtatta gaaaacaaaa cgaaacagcc ggattttcag ttgactaaat
    36301 ctatcaaaga acaattacta gaagttgtaa tggattatat gattcctcag aagttccatt
    36361 atgtcgatct gctgcctcag acagttaatg gaaaaatcga tcgaaaaaaa ttaacagctg
    36421 aggtgaatcc tgaatgatgt tacctcatat gatcccttat gcagagccgt tttatttttt
    36481 tcttttagcc attgcgcttg taccgatcgt tctatcttta ttgatatggg aaaaaagatt
    36541 accagtttat caatcagcag tgacactttt ctttttattt gtcagctttg gaggtaatta
    36601 ttggcaacag ggagcggcgc tgattgggta tgttttgtgg cagacgatac ttgtatgggg
    36661 ctattttgtt tatcggcaaa ggaaaaatca gacaggatgg ttttttgccg ccgttatttt
    36721 agcgataatt ccactgtttt taacgaaagt gactcctttt tttgaacatg gaaaagcatc
    36781 gttacttggt tttttaggaa tctcttattt aacctttaaa tctgtacaaa tgatcatgga
    36841 aataagagat ggcatgatca aaacatataa gccatttcgt tatatccagt ttctgctgtt
    36901 ctttccaaca atctcttctg gaccaatcga tcgctatagg agatttgaaa aagatcagct
    36961 ccatccgcct gttcgtgata aatatgttga tcttttggga aaaggcgtcc aaaatatttt
    37021 tgtcggtttt ttatataaat tcattatcgg ttattatttt ggtcaagtac tgcttcctgt
    37081 agtgggacgc attgccttac agcatggtgg tatttcatgg gcattagtag gttatatgta
    37141 cgtgtacagt atgtatttgt tttttgattt tgctggctat agcttatttg cagtcggtac
    37201 aagctacatg atgggatatc agacaccaat caatttcaat aaaccttttt tatcatggaa
    37261 tatcaaagaa ttctggaatc gttggcatat gacgctgtcc ttttggttcc gtgattatgt
    37321 atatatgcga ttgatgttct tcatgatgaa gaaaaaattt tttaaaagca agatcgtaat
    37381 gtcaaatatc ggttattttg ctttgtttct tctaatgggt atatggcatg gattgacgtg
    37441 gtactacatc gtatatggac tgtatcatgc ttgtctgatc tgtctgacag atgcatggat
    37501 ccgatttaag aagaagcata aagagcagtt gccttctaac aaatttacgc atggcttagc
    37561 cgtatttttg acatttcaag cagtatgttt tagcttcctg attttttcag gatttttaga
    37621 taaacttttt tttagctaac gaatagtgac atattaattt atataacaaa ggagaaaagg
    37681 aaaatggaag aacaaatttt atcaatctta gaagaaataa caggaacaga tgaagtaaga
    37741 aaaaatcgtg atattgatct atttgatgaa gggttgcttg attctttagc atctgttcaa
    37801 ttattagtcg agctagacgg agagctggat atccaagtcc ctgtatctga attcgagcga
    37861 gaggattgga gcacaccgaa taaaatcatt caacaagcaa ctgcattgaa gggttgagta
    37921 tgaaaaagaa actttttgct attttcggcc cggttgtttt agctgttggc ttactgtttg
    37981 tgttttttac ctcctcctat caagtagata tctctaatcc agaaacgatt agacgtgcct
    38041 cttcttccat gtcagtgaac gtgttgaaag gagatgcgat aaaaaattat gcattatctg
    38101 aaaaacaata tattcctttt tttggctcaa gtgaattaag cagaatcagt ccatttcatc
    38161 cgtctgttct ggcagaaaaa taccagcgaa actatcgtcc atttttgctg ggagcacccg
    38221 ggacacaatc gttaagccaa tatatgatga tgcgttctgc aggagatgct atgaaaaaca
    38281 agaaagtcgt ctttattatt tctcctcagt ggttcgttaa aaatggtgta aaaactgatt
    38341 actttaatac gtattattct gaacttcaaa catacgactg gttgttttca atgaagaaag
    38401 taacgcctgc tgatcgctat ttagctagaa gattattaac tttctcaaaa gtaaaagaga
    38461 atgatacgtt gacggcgatt ctgcaaacga tcaaaaaagg gaagctacct ttacctgaat
    38521 ctttgaatca gctgcgcagc caatggaaca tgctgaagag agaggatgaa gtattcagca
    38581 atattgggtt gaaagatcga cagcaaaaaa tcgatcatga gtcaaaacga ttaccaaaac
    38641 aataccaaga aacagaatta tccattttag caaatcaaat tggagaacga gaaacgacga
    38701 ataatccatt cgggttgaaa aacgattttt atacgcatcg tatccgagcc cacgagccag
    38761 agctcaaaca gtcacaaaaa aattgggatt atcgtttttc gccagaattt tctgattttc
    38821 agctggtctt ggatcaatta gcaaaaaacc ataacgaagt attgttcatc atcccaccgg
    38881 ttaacgagaa atggagcgat tataccggac tatctcaaga aatgttacaa ggatttgcca
    38941 aaaagatcaa atttcaatta aatagccaag gattcaatcg aatagctgat tttgtcaatc
    39001 aagcgggtac caactatttt atggaagaca cgatccatct aggctggaaa ggctggttgg
    39061 ctgcagatca acagatcaga ccgtttttag aagaaaatca tattactgct agtaagtatc
    39121 atcttgacga tgcttttttt agcaaatctt ggcaacatca aataccagat aaactacaat
    39181 taaaataaag aaaacaggcg agtgttgctc gcttgttttc tttatttttg aatattctga
    39241 ataaaagata aaaataccaa gtttgcctga acaaaaatca taagcaatgg tataatcaca
    39301 atatgaatat agaaatgggg gaaaacattt ggatacaaaa gaagaacagc tacttattga
    39361 tttaacttct gcaaaaggag tacctggaaa tgaagaagag gtaagagaag tattcagaga
    39421 atatgccaaa ccattcgctg atgatatttt ttatgacggt ttaggcagtg tgattgccaa
    39481 acatggtgct ggcggaggcc ccaaggtctt tatttctgga catatggacg aagttggttt
    39541 tatggtaaca aaaataaccg aaaaaggatt cttggaattc caaactcttg gaggttggtg
    39601 ggggcaagtc atgcttgctc agcaagttga aatcaaaaca cgtgaaggga aaatcgtcca
    39661 cggtgtgatt ggttccaaac caccacatgt tttaacacct caagtaagaa ataaacctta
    39721 tgaaataaag gatatgttca ttgatattgg cgcatcaagc caagaagaag ccaaagagtg
    39781 gggcatacgt cctggagata tggtcactcc atatattgaa tacaaacgaa tgaatggcag
    39841 taaatattta ctggcaaaag catgggataa tcgtatcggg acagctgttt ctctgcgagt
    39901 attagagaat ctatctaaag aagcacaccc aaatgtttta ttcgcaggaa gcgatgtgca
    39961 agaagaagta ggcttgcgag gtgctcgaac aagtacacac cttgtaaatc ccgatattgc
    40021 ttttgcttta gatactggta cagcaggaga cactccagga atgacaccaa aagaagcaga
    40081 ttcgatttta ggaaaaggcc cacagatttt gatttttgat gcatctatga tcccgcataa
    40141 aaaattattg aattttgtta ttgatgtagc agaggaaatg gatattccat tccaatatac
    40201 agtgatttct ggcggaggaa ctgatgcagg acaaatgcat ttgacaagag atggtgttcc
    40261 gtctgtagcc attaccgttc ctgttcgtta tctgcattcc catacatcag tgattcatga
    40321 agatgattat ttgaatacag tgaaactagt cacagaatta gtgaaacggc ttgatagtga
    40381 aacagttgaa aaaattcgga gttatgaata atgaataata gaatacctat ttctgaatat
    40441 cggaaaaaag cacgagtttc tttggaaaat caatggggaa tcaatacttg gcttgttttc
    40501 ttatctataa tatttacgag cgtcatccag aacttttttg acggtttcag tccttttccg
    40561 gaagaaagca tgcagcagca cttattgagt tttctagtcg ataatttact tttattcgca
    40621 ttcaactatg cattgtttta cattgcgcta gtagttgtac ggggtggcag accaaaaagt
    40681 aaccttttat tttcagtttt ccaaagaaag tattttggtt cgatacttgg catcaatttg
    40741 atcaattcag tcatcaactg gctactcaac agtttaatct ttctgcccat gtttttaatc
    40801 agcggagtga gtacttatat gcagctgctt ttcagttcaa atgcggtacc tacgggtgtc
    40861 atgccagaag attttcttga tatcagcttt gttttatcga tgatagggat gatttttgtt
    40921 tcagcactca tcatgagtat tgttggcggt ttattccaat ttgctgcatg gacaaagttc
    40981 gactatcctg atttgtcggt tattcaatgt ttgaaatatg gctggttttt attgaaagat
    41041 agaattggta cgtatatttt gttgcagctg tcttttatcg gctggtattt gctaggagca
    41101 atcgctttgt tcatcggtct tttgtgggtc attgtttatg tgaatgtgtc agttgcagaa
    41161 ttttatgaac aagcacggat tgaaaaagga ggtccagacg cctattttac ttagcatgta
    41221 agcagtttat tcatcaaaaa aataactttt gtggtaggat aaatctttga gggggaatgt
    41281 acgatgaaat taactgtttt aggatgctta ggagcgtatc cttataaaaa tcaagggaca
    41341 acaggttatt tgcttcaatc tgacgggttc aatttgttga tcgatgcagg tagtgctacg
    41401 ctgatcaaat tacaagaaca ccttgatcca cttgatctag atgctgtgat ccttagccat
    41461 tatcatcatg atcatattgc tgaccttggt gtattacagt attactggca acttcatcct
    41521 aagagaaatc cggagacatt gctttcaatt tatggacaca caaaagattc cattcacttt
    41581 gaagaattga cgttggaaaa tgtctcaaaa ggacatcctt attttgaagc tgaagaatta
    41641 aaaattggtc cttttctgat tacttttatg gaaacaatcc atccagtagt atgttacgcc
    41701 atgcgtatcg tagaagaaac aacggggaaa gtattcgtat ttacaggtga ttcaggttat
    41761 ttggaaagtt tcgctgattt tgcgaaagat gctgatttgt ttctagcaga tacctatctt
    41821 tttgaaggaa atgaacatca tcatgcccat ttcacttcaa aagaagctgg agaacttgcg
    41881 aaatcagcta acgtaaagaa acttgtactt acacacctac ctcagtttgg tgatttagaa
    41941 caattgaaaa atgaagcgca aaaggcagca ggcaaaggga ttgatataga acttgctgca
    42001 gttgacaaag tgtttaacat ttaggaggaa gaacatgatt tttgtaccaa atgaaaacca
    42061 cgacccaaga atcaatttag caattgaaca atatttgctg caagaaatgc ctttagatga
    42121 acctattttg ctcttttata tcaatgaacc ttcaatcatc atcggcagaa accaaaatac
    42181 gcttgaagaa atcaatcgag attatgtgga agaaaatggc attcatgttg tccgtcgctt
    42241 aagtggaggc ggagctgtct accatgattt tggaaacctg aactttagtt ttatcatgcc
    42301 tgatgatggg gattcattcc gtgactttgc gaaagtaaca cagccaatca tccaagcact
    42361 tcatgaattg ggtgttgctg gggcggaatt gaaaggccga aatgatctag tcattgatgg
    42421 catgaaattt tctggcaacg cgatgtatgc taccaacggt cggatgtttg ctcatggaac
    42481 aattatgttc gactctgata tcaatgaagt agtcaatgca ttaaaagtaa aaaaagacaa
    42541 aatcgaatca aaaggaatca aatcgatccg ttctcgtgta acgaatatca aaccatttct
    42601 tccagaagaa aagcaaaaaa tgacaacaga agaatttcgt caagaaatct tgttgaaaat
    42661 tttcggcgta gagtcagtag aagaagtaaa aacgtacgaa ttgactgatg aagattggaa
    42721 aaaaatcaat cgaatctctg aggaatatta tcgaaattgg gattggaatt acgggaagtc
    42781 tccagatttc aatttctctc gccaaaaacg tttttctatc ggttcgattg aagtccattt
    42841 gaatgtctca gaaggaatga tcaaagaagc aaaaatcttt ggcgatttct tcggactagg
    42901 agatatctct gacgtggaaa aaaaattggt tggacagaaa tatgataaag catctctatc
    42961 aaaaatagtc agccaaattg atgtgaagaa atattttggt gcgatagaac cagaagaatt
    43021 gcttacctta ctttattgaa aataaaaaat agcggaatcc acatatatgg attccgctat
    43081 tttttataca ggattcgggt aatttcgaga gagttgaaca gtgatccgat caatgaaagt
    43141 atctttttcc tgcgttagtg ttcctgtgta acgatccgct acgtctttca tcatcattaa
    43201 gtcaaaacgt tcctgatcat ctaattcttc tgtgatgcct cgactaaata ggtttgtatc
    43261 gtaatgtgca ctatgttcgc aacaaatgac gaaatgttcc tcttgttcgt gtatcgacaa
    43321 ataaattttt cgatacagtg gattttcgat ggcatgagtt tctctaaagg aatgctctaa
    43381 agcgtggatc aaaagttgag ccagatcgtc atcgttgata ggaagttctt cgggaagatc
    43441 tgcttggaag gttatctgaa tattccgttt ggcagctaac ttctggtaac gcgctaagat
    43501 aagatttgtc acctggtggt tgcaaaaaga acacagcatt tcgaaatttt ttgcttcttc
    43561 taatatcttt tccacatagt ttttagcttt tgtttggtcc gtatgcatta aatcttgcag
    43621 attctccatc tcttggacaa attccaggcg aagtgtattt acttcttgga tatgctgtcg
    43681 aagctgctca taatgttgct ccaacaaatc atttttgatt tttaagaaat ctacttgtct
    43741 ccgatctttg tcggtattta agatgtgatc gattagattg tagctgataa taacgattag
    43801 aatgataata tataaaatcg tagaccaatt gatcgttgtt tgatagatac ctaatgccgt
    43861 ttggatgtaa atcaagcaat ggataatggc taccaagacg atccaaggtg tacaagctac
    43921 ataaaaacgg ttattcttat atgcttcgcc aatgcatgca agtgaagtga ctaaagtgct
    43981 tataacacta gcaatattag caatcggcat taattcagga agttctgcag gagtaaaagt
    44041 tgcataaagc agaacgccga taaaaatcaa aagttggaac gtacaaaaaa ttccgtacca
    44101 ttttttatag taaaacattc ggctccaata gtagcagatg atgaaagctg acgttaagat
    44161 aataaataaa tgagataaaa aagaatgaac gaatggttca aaaagaatac cacttaaaat
    44221 atcttctgat acagcttcaa atcctaatgc caaagcaaag caacttaaga gaataagtga
    44281 gtattcaaga cgtttttgtt tatacatcaa gtagatcgta tagaaaaagg tccctatagc
    44341 gataaaaaca gcgattatca tcgtcaatac ttgcggcact gattgtgaat aaataaaact
    44401 aatcaatggt ccagttgttc ctagataaac tttcggtggc agtccagcat agttctgata
    44461 aggagatgag actgtaaggg tcaatttttt tccgatataa tttgctggta aatctatcac
    44521 agccaatgag agtccgggat tgctttcttc ctgataatct ttccgttggt ataaaatctt
    44581 ttcgcctaga tatacagtaa tccattgatg gtttcctttg attagtaaat ttgcttcgtc
    44641 caacggtttt tcaagtgtcg tttccataat gaatgtttcg tctttatcca cggtaggaat
    44701 cgtacgagtg tacttgctgt caaacttaaa atcaggatca tcttttgtat agaaaaccca
    44761 cgaatctttt atttctttca tttgattgct gtccgcagga gaaaaaataa aataaaagaa
    44821 ataagcaatt cctgttaaaa ataacaatga taatataatg attatgctcc actttatacg
    44881 taaataaaaa gattcttttt tattatatat gttattaa
//
LOCUS       DAJSAW010000020        43878 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJSAW010000020 DAJSAW010000000
VERSION     DAJSAW010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.020"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            277..1602
                     /locus_tag="ONT64_001183"
     CDS             277..1602
                     /locus_tag="ONT64_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR2302615.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            1595..1945
                     /locus_tag="ONT64_001184"
     CDS             1595..1945
                     /locus_tag="ONT64_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302616.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            1942..2121
                     /locus_tag="ONT64_001185"
     CDS             1942..2121
                     /locus_tag="ONT64_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302617.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            2257..2547
                     /locus_tag="ONT64_001186"
     CDS             2257..2547
                     /locus_tag="ONT64_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HCR2302618.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            2900..3703
                     /locus_tag="ONT64_001187"
     CDS             2900..3703
                     /locus_tag="ONT64_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR2302619.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            3690..4016
                     /locus_tag="ONT64_001188"
     CDS             3690..4016
                     /locus_tag="ONT64_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302620.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            4365..5405
                     /locus_tag="ONT64_001189"
     CDS             4365..5405
                     /locus_tag="ONT64_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR2302621.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            6241..6573
                     /locus_tag="ONT64_001190"
     CDS             6241..6573
                     /locus_tag="ONT64_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCR2302622.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            7164..7493
                     /locus_tag="ONT64_001191"
     CDS             7164..7493
                     /locus_tag="ONT64_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302623.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            7480..7821
                     /locus_tag="ONT64_001192"
     CDS             7480..7821
                     /locus_tag="ONT64_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HCR2302624.1"
                     /translation="MKNYDPNSRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDQGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            7935..8174
                     /locus_tag="ONT64_001193"
     CDS             7935..8174
                     /locus_tag="ONT64_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302625.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            8241..8480
                     /locus_tag="ONT64_001194"
     CDS             8241..8480
                     /locus_tag="ONT64_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302626.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            8566..9237
                     /locus_tag="ONT64_001195"
     CDS             8566..9237
                     /locus_tag="ONT64_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCR2302627.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            9290..11266
                     /locus_tag="ONT64_001196"
     CDS             9290..11266
                     /locus_tag="ONT64_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR2302628.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGAAQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            11279..11935
                     /locus_tag="ONT64_001197"
     CDS             11279..11935
                     /locus_tag="ONT64_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR2302629.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPAVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSIPLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKK
                     "
     gene            12031..12858
                     /locus_tag="ONT64_001198"
     CDS             12031..12858
                     /locus_tag="ONT64_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302630.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVTQQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETQTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIVL
                     LTPVSDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            12868..13116
                     /locus_tag="ONT64_001199"
     CDS             12868..13116
                     /locus_tag="ONT64_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302631.1"
                     /translation="MKIIKMLVFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINKLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            13159..15258
                     /locus_tag="ONT64_001200"
     CDS             13159..15258
                     /locus_tag="ONT64_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR2302632.1"
                     /translation="MENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNVKLDFKYAGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMDRLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNKVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIALDHYKQFSIQDPVEA
                     RLTYKEGSARVIDMSTGKDITSEGILTYDSHSRTLKWEASADFLNNNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQVIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(15296..17353)
                     /locus_tag="ONT64_001201"
     CDS             complement(15296..17353)
                     /locus_tag="ONT64_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HCR2302633.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(17467..17679)
                     /locus_tag="ONT64_001202"
     CDS             complement(17467..17679)
                     /locus_tag="ONT64_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302634.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            17866..18333
                     /locus_tag="ONT64_001203"
     CDS             17866..18333
                     /locus_tag="ONT64_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302635.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            18330..18572
                     /locus_tag="ONT64_001204"
     CDS             18330..18572
                     /locus_tag="ONT64_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HCR2302636.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            18590..18892
                     /locus_tag="ONT64_001205"
     CDS             18590..18892
                     /locus_tag="ONT64_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302637.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            18963..21017
                     /locus_tag="ONT64_001206"
     CDS             18963..21017
                     /locus_tag="ONT64_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302638.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            21017..22849
                     /locus_tag="ONT64_001207"
     CDS             21017..22849
                     /locus_tag="ONT64_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCR2302639.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            23562..25460
                     /gene="ltrA"
                     /locus_tag="ONT64_001208"
     CDS             23562..25460
                     /gene="ltrA"
                     /locus_tag="ONT64_001208"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR2302640.1"
                     /translation="MTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSNFKNLMK
                     FIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKDYRPRKV
                     KRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNCSTENAI
                     ADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRKMLKAPI
                     VMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHYRKNGAW
                     DKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERLKLPISM
                     EKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIKKIQYAG
                     AKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARGMTKNGI
                     YGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRKLIHQEQ
                     QAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEIECHHIN
                     PYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTYRKKAAN
                     FPISIKMN"
     gene            25577..26377
                     /locus_tag="ONT64_001209"
                     /pseudo
     CDS             25577..26377
                     /locus_tag="ONT64_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            26374..28905
                     /locus_tag="ONT64_001210"
     CDS             26374..28905
                     /locus_tag="ONT64_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302641.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            28955..29287
                     /locus_tag="ONT64_001211"
     CDS             28955..29287
                     /locus_tag="ONT64_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302642.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            29288..29881
                     /locus_tag="ONT64_001212"
     CDS             29288..29881
                     /locus_tag="ONT64_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302643.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            30044..31738
                     /locus_tag="ONT64_001213"
     CDS             30044..31738
                     /locus_tag="ONT64_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR2302644.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNGATCSAVKSCGLQVV"
     gene            32426..34303
                     /gene="ltrA"
                     /locus_tag="ONT64_001214"
     CDS             32426..34303
                     /gene="ltrA"
                     /locus_tag="ONT64_001214"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR2302645.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            34434..34811
                     /locus_tag="ONT64_001215"
                     /pseudo
     CDS             34434..34811
                     /locus_tag="ONT64_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            34811..36016
                     /locus_tag="ONT64_001216"
     CDS             34811..36016
                     /locus_tag="ONT64_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCR2302646.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            36032..36676
                     /locus_tag="ONT64_001217"
     CDS             36032..36676
                     /locus_tag="ONT64_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302647.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            36687..37493
                     /locus_tag="ONT64_001218"
     CDS             36687..37493
                     /locus_tag="ONT64_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302648.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            37515..37745
                     /locus_tag="ONT64_001219"
     CDS             37515..37745
                     /locus_tag="ONT64_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302649.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            38291..38566
                     /locus_tag="ONT64_001220"
     CDS             38291..38566
                     /locus_tag="ONT64_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302650.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            38608..39030
                     /locus_tag="ONT64_001221"
     CDS             38608..39030
                     /locus_tag="ONT64_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302651.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            39049..40641
                     /locus_tag="ONT64_001222"
     CDS             39049..40641
                     /locus_tag="ONT64_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302652.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            40653..40988
                     /locus_tag="ONT64_001223"
     CDS             40653..40988
                     /locus_tag="ONT64_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302653.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            41135..41329
                     /locus_tag="ONT64_001224"
     CDS             41135..41329
                     /locus_tag="ONT64_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302654.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            41361..43733
                     /locus_tag="ONT64_001225"
     CDS             41361..43733
                     /locus_tag="ONT64_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302655.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNSQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT        16235 a         6763 c         8838 g        12042 t
ORIGIN      
        1 tcaaatgttg taaggatttt attcatttgt taatattaaa gaacggaact ccaaaaaagt
       61 ccgtactcta atttgcagtc tctcactctt ttagagtgag agtttttatt agattttcaa
      121 attaattagt aaagtgatag aataaattga tcatacaaat cataagaaga agcagggtga
      181 tagcatacct atgcttcttt ttttatgtat ttagctattt cttctttaca acatcgaaca
      241 tatgtttgta taatgtggaa gaatggagtg ttatttatga tggattattc aaaagagcca
      301 gtaaatgatt actttttaat cgatatgaaa agcttttacg caagtgtcga atgtatagaa
      361 agaaatttag atccattaac aacagaactt gttgttatga gtcgatctga taatactggt
      421 tcaggattga tattagcttc ttctcctgaa gcaaaaaagc ggtatggtat tacaaatgtg
      481 agtagaccac gtgacttacc acaaccattt cctaaaacac tacacgttgt tccaccacgt
      541 atgaacctat atatcaagcg aaatatgcag gtaaataata ttttcagaag atatgtggct
      601 gatgaagatc tactgattta ctcgatcgat gaatcgattt taaaagtgac aaaatcactg
      661 aatcttttta cgactgaaga aacacgaagt cagcgaagga agaagctcgc tcaaatgatc
      721 caagaacgta ttaaagaaga gttaggtttg attgctacgg taggcgtcgg cgataatccc
      781 cttttggcta aattagcctt agacaatgaa gcaaagcata atgaaggatt tattgcggaa
      841 tggacgtacg aaaacgttcc tgagaaagta tggaatattc ccgaaatgac ggacttttgg
      901 ggaattggtt ctcgaatgaa aaaaaggctc aaccaaatgg gaattttgag cattagagac
      961 cttgctaact ggaatcctta tacgattaag aatagattag gtgtgattgg attacagctg
     1021 tacttccatg ctaacggaat cgatcgaaca gatatagcaa tcccgccaga accgacaaag
     1081 gaaaaatctt atggtaattc acaagtgtta ccacgagatt atactagaag aaatgaaatc
     1141 gagctggtgg taaaagaaat ggcggaacaa gtagccattc gtatcaggca gcataattgt
     1201 aaaacaggat gtgttcattt aaacattggt acgtcgatct tagaaacgag accaggattt
     1261 tcacaccaga tgaaaatacc tatcacagat aatacaaagg aattgcagaa ttattgtctt
     1321 ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc acgttggaat aacctatagc
     1381 aagcttgtgt acaccgattc gcttcagctg gatttattta gtgatccaca aaagcaaatt
     1441 aacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg ttttacgtcg
     1501 attgtacatg cttcttcaat gttagaaagc gctagaagca ttactagaag cacacttgtt
     1561 ggtggtcatg cgggcggaaa tgggggaatt aaaaatgatt gattacaatg atcgtggacg
     1621 tttaaagtgg gcagggtttt atttatctga tcatactgaa gaaattgatg ccgacaatga
     1681 acaacgaacc agtcaaaatt tagcaaaaga acaaatgacg actgaagaga ttgcggaagt
     1741 attgaacgct gcagtcataa aaaatagatc agtatctatt caaaaagaag aacgaaacgc
     1801 tgaaggatac tatccagaag atattgttgg caagatctta ggtagtgatg agctggggat
     1861 ctacgttgga aaagacaaaa ttcactacga cgaaatacgt catatcgaat tttaccagga
     1921 taataaatgg agtcgattag aatgaacatt atccagcaat atgagctaaa gtatataacc
     1981 tttgatcagc tatcagaaga gatttggggt tatggccaac gtttgataaa tgaggttggt
     2041 gttgagcgct tctctttcta tgtagaagct gcagcaggtt atcataattt taggttttat
     2101 attttcccgt tatatatata aggtatttta cggattctat agagttatct ctatgaatcc
     2161 ttttttgttt ttacaaatag gagtagatac tagaataatg cgtgtgttaa actattattg
     2221 caaacgcaat aataaaagaa agtaggctta cgcaccatgt ctatcaatac atttgtttac
     2281 tcgcacccga tcaatgttta cattatcaag aatttaggaa tcactgtaga acaattttgt
     2341 gagttatatg cctacccaca aggaacggtt gcaagctgga taacccgaca acgtagaata
     2401 aaatcattac ctgcttcatt cgtttatgat ttaagcctgg cctctagttt aaacatgtct
     2461 gatgtttatg aaaagctcct gtctttagaa aaagaatatg attccttcaa gattaaacat
     2521 gataaaaaaa taaaaaaaca tatataatat caatcacaat tgtataatat tacgatatct
     2581 taatatcatg atatcgtaat attgtttttt tctaagaaaa tgcactataa gaagggaatt
     2641 attagtaaca atatagacag tttaataatg tttaaattat tctagttgct gctcgcaaaa
     2701 catatttctt atgattaata aaaaatttta aagtaataaa aagtgattat tttactttcg
     2761 aataacatat gaataatctt tatatccgaa ccgatatata tctttaaaac aaaataatca
     2821 cacaatatta caatataatg catatacgat attgcaatat cacaatatta tgatataatt
     2881 atattataat ataagatgaa tgggggatat tttaatggca agaatattgt cagtagccaa
     2941 tcaaaaaggt ggagtttcta aaactacgac tagcgttaat ttagcagcat gtttgaaatt
     3001 tgtttacggt aaaaatgtac ttttagtgga cattgatcct cagggaaatg ctacagataa
     3061 tgtaggtttc gatatagatg gaacaaacca acctacaatt tatgaagtat taaaagggga
     3121 ggctgacatt acagatgcta ttctggacta taaaggaatt gatgttttgc cagccgacat
     3181 agccctttct tctgcagaga gggaatttac tcaagtagga agtgaacaca ggcttaaaag
     3241 agtacttcaa ccaatcaagg acaactatga ttatattata attgattgtc caccaagttt
     3301 aggaatgctt acagtcaacg catttactgt ttctgatgaa attatcattc cagttgaagc
     3361 tgcgtatttc tcattaaaag gactaataaa gttaagtgaa actattgaaa cggtaaaaga
     3421 atatacgaac gacaagcttg aagtacgagg agtcctattt actaagtata ataagcgtta
     3481 taacatttct aaagaaatga cgactgcagc aaccgaaatt tctagagtca ttggtgcaaa
     3541 aattttcaaa acttatattc gtagaacaat aaccgttgac gaagcgcaag ctgctggatc
     3601 agatctaatc aattttaata aaccctcaac agctgaagat gactataaag catttacaga
     3661 agaatattta aaagaagttg gtgattttga tggcagaaga taatcgcagt caagctgcag
     3721 taagtagact actaggaaat aaaaataaaa agaactacaa accaaaagaa gtagaagaaa
     3781 tcgaattaac caagaaaaat ataagtaaag tcataaaaga tcaaggaagt agtgtaagaa
     3841 cagcagatag cattcgtata aatccaattc taatgtcagc actcaaatat tggacgacta
     3901 ttgctgaatc agacaaaagt aaaccagacg ttatagaaga tgcgctatta aaatccatac
     3961 ctgaagaata cttgattgaa ggatataaac tagcaaagaa acaaaataaa atttaattta
     4021 cattcaatat cacgatatca taatatcgtg atattttttt tataaaaatc agtagtatca
     4081 aagctgataa gtcgcttatc ttcatattgt aatattacga tatcgtgata taatgttatt
     4141 gtaatatcgt gatatagtta ccatgagctt aaaccctgaa atagagcggt ttgctttaat
     4201 ttatttttta aggtaaacta aaaaaagata aaaaaagact actgctttaa caaagtttgg
     4261 cgacttatca gcagtagaaa aatatatagt ggatagatat atttgtttaa ttcattgtaa
     4321 catagttttg aacaaaacgc tatcctacag aaagaggatt ggctatgaat gattttaatt
     4381 attacaaatc aaaagaaatt tatcgtgaaa aatattatca aatgcctaag gtatttttta
     4441 ctaacgaaaa atacatggat ctttcaaatg atgcaaaaat tgcttatatg ctacttaaag
     4501 atcgatttga ttattcagtc aaaaataact gggtagattc tgatgataat atattcttca
     4561 tattcacagt tgaagaatta atgaaattgt tacaatgccg cgaaggtaaa gtatctaaga
     4621 tcaaaaaaga attagaaaaa gcaggtcttc tgaaacagaa gagaggaaga gtaaataaac
     4681 gtgatggtaa gattgaatct atgcctaatt tattgtactt aggcaagcca gaagttacga
     4741 atcaagacgt ctttaaaatc atggaagagg aagacaatac tgattccacc gtaattgcga
     4801 aaatcgcaaa tacggcaaaa ccgcgaaaaa tcaatgattc taccgtaatt gcgaaaatcg
     4861 caaatacggc aaaatccagt catatcaagg actcaaccgt aattgcgaaa atcgcagaca
     4921 atctattcta ttctaattct atagacacta atagacacat aatagacact gaaactgacc
     4981 agttacaaaa tcaagttttg ctagataact tcgtcgacat catgcaagag gacagtataa
     5041 atacatttgt accagaaaat gtattaaact tgataaaaac attttcttca agttacagtg
     5101 aagctcaaca aaccgtccaa actattcata atgcaaagaa aaaagctgaa gaattagagc
     5161 aaactattgt cgtttatgaa gaactggaat cttatggaat tgatgctgac aaaggattat
     5221 atatgacttt actgaaagct tatcaaaaac aaaaaactga aaaagtaaac aatttgcaga
     5281 atctaatttt cgtttatgta aaaaattggt ttgtcgaaaa agctattgcg gcaaagcttg
     5341 caaacgaaca aagagaaaca tttaacgaat taccaacagt tagtacggaa aattggttag
     5401 agtagttgat aaaaagcaat tactataaaa atgatcaatt ttaaattaga acttttttat
     5461 gattttaatt ttattacaaa tattacaaat aaatagttag tattccggag tttttagatt
     5521 aaaagtttat gaaaacaatc acaatcgtag gttttaaatt cataatcgtt agatatttta
     5581 gttatttttc actcaaaaaa atagggaact agtgtttgca tatgtgcttt tttgagttat
     5641 aataattcat gaagagaggt aattcactta tttccccaag ctgaaaagcc tgatattact
     5701 tcttttcagg cagtgctgct aatattcaat tacacttttt tgtatatttt tagttgtttt
     5761 aaatttaatt gtctgctatg cttgtgatga attgattttc gttctgcact tttggacgat
     5821 aaatttggac aattgaaaat acaaaaaaaa gaaaggccct gtgagactcg gaaggctggt
     5881 aaccttcgtc gacgtccaca ggtggcatta agatcactcc cttaatgcgt ttacctttca
     5941 gtgttacggt attacccgta acggatttag tataacagat attcaaaagt gataccactt
     6001 tttttggctg tgttaaggtt aaaaatacgt gatattacga tgaatctatg cctaagcata
     6061 atgttataaa aatgtccgtg tggctctttg ccgtatgacg aacgctgaaa ttagagggaa
     6121 cgcatgtttg gatgcgttcc ctcttttttg tttgtcatga acgaaaatca attctctatt
     6181 tttgaaaaat aacaagaaaa acttgttaaa aatatttaaa gtaagtgggt ggattatttt
     6241 atgtcatata caggaatttt gagtctcgaa gatatttgtc actacggtaa acggtgtacc
     6301 gctacagaaa agatcacgaa gaaactatca actggtcaaa gtaaaaccgt tgtgcagtgt
     6361 aagaaatata taatccagaa agacaaagtc tcagaagaaa tgatttatta cattgggaaa
     6421 caaaaacaaa taattttaaa agatcctatc cctttaaaag agctatatcc aacaatcaaa
     6481 catgtttatg accaaaacgg tgtactgatt ggtcgaagaa aaaatggagt cttgcgttgt
     6541 accgcaaaag ggatggggcg tttaatcagc tgaattgatc attctcataa cttgtatttt
     6601 atcgtttttc gcttgcttga aaatattgtc gagacttcta aaggaactta gaatcactag
     6661 aaaaataagg agtagacttg atctatcaac aattcctttc ggggggtgac ggggtaggtt
     6721 tttacgattg gtaacgtaaa aaattggtca atcgagtaaa tgcccttgtt tggatagtgg
     6781 tgaaatgaat ttgtgtaaat aatatgcaca gagtaaacaa gagggaattt agaagttaga
     6841 caagttagaa tagcgcctag attaatcatg agcagctact aagggatcta ggctttgacg
     6901 caaactcatg gaataaggag taggtcttct cgtaatgggg aggacgcaca agaaattgtg
     6961 tcgtgtgctg cagtacatgt agatacttgc gaagacgttg atctttggat caatcgagca
     7021 tttaccgttt tctactaggg tttctcgttt cggacgccat aaacgagtcg atttatttta
     7081 tatatttttg agtgaacgaa aaacgataaa aatattcgtt atgaagtaaa actaatttag
     7141 tagatagcaa aggtggtaga ggaatgattc aaaaatttgc gaaaatgagt aaaactgcat
     7201 ggattgaaaa gcttgaaaat gacttgagtg tcatgcgatt agctccaaag aaattcaaac
     7261 gagagattgc agaaaaagaa gcaattttgt cggtattgaa tgcggtagac gataaatgtc
     7321 cagtaaaaat tagtttttca gatatagcaa caattatcga aggtatttat tatggtgaag
     7381 ttgatgaaga gatatcttct acaaaagatt tattagattt tttggataaa aacaaagata
     7441 cctttatagt actaactgat tatgaggagg ttacaacaaa tgaaaaatta tgatccgaat
     7501 agccgatggg gaacacatac gattaaggtt tctttccaac gttgggatta caaaggattt
     7561 gtgacgttta gaagaggcgg aaattgtaaa ggattagatg ttttagcatt agacgaggat
     7621 gatttatacg atcaaaaatt gactgataat ccaattggat tcggcttgct acctgaagat
     7681 gatcaaggta atgaatggtt taaaatgacg cttatgaatg ataatggaga cgaattatct
     7741 gttgaagata cttggtcata tttgagtgat tatattgtca gcgttgaaat cattgaattt
     7801 gtagcggata aagaagaata aaaactggag gaataaattt tacagataaa ggcttagaag
     7861 gagtcacgac gattaataaa aaggtagtat taccttaggc attgagtaag gaatttattt
     7921 aggaggaaaa ttagatggcg cgaccagttt tagaggcgat ggatatggat actttgacaa
     7981 aaggaaggta tgtacagctg ttagctcgtg gttattcacc taaagaagta ttcaaatccc
     8041 tgtctaaagg aacagatatg gaaaaagaaa gattacgaaa agaattcgat tattggagaa
     8101 cccataatgg aatcaaggat ttgaaaccag cacggccagt aattaaacga aagaaaaaga
     8161 taacccaaga ctgaagtgta gagcgtatgt gatcataggc ttttttattt ttgcaaaaaa
     8221 caaggaaggt aggaaaactc atgaattatg aagaaatcga aaacagaaaa aaagtgtcga
     8281 aagaaatgga agaaaagttg ctaaaaacga tgaaacaaaa acaccttaaa agattatctg
     8341 tcatgcaata tatcaatgat atgcagataa caggaaaaga gaaagcttgt ttgttaggtt
     8401 caatgaaaaa ctttgaacag ttgagacgaa cttatgtgaa aaaaagttct aattgtcaac
     8461 tattgttaga agtctcatga cattcattaa tagaattcat ttttaaaact ttttctatgg
     8521 aaatgagaaa aagatttttt gttttgtctg aaagggggta gctggatgag aaaatggtta
     8581 tttggttttt taggagtcgc tttaatcgtc ttttgcagtg tgtttggcta tgttagctac
     8641 caaaaacatg aaggaaaggt gtttaagcaa aatatagaaa aaaagatgcc cgtagatcaa
     8701 atcaatgaac atgcaaagag ttataaggag gatgcaacga acgtcaataa tgatatgtct
     8761 ttggggcaaa tgttgtccat acaaaaagaa gcaatcgaaa tgggtgtcaa taaacaagcc
     8821 tttgcacaaa ttcagatacc tgcacttggt ttagcattgc caatttttaa aggggcaaat
     8881 caatacactt tgagtcttgg agcggctact tatttttatg aagatgccga aatgggcaaa
     8941 ggaaactatg tcctagcagg tcataacatg gaaatgccag gtgttttatt ttctgatatt
     9001 caaaagttgt cattagggga ggtgatggac ttagttagta acgatggcgt ctatcgttac
     9061 aaggtaacgc gtaaatttat cgtacctgag tactttaaat taatcgatgg cgttcctgaa
     9121 gagaactctt tcttgtcttt accaaagaaa ggagagaaac ccttattaac gttgtttact
     9181 tgtgtttata cgtcacaagg aaaagaaaga tacgtggtac agggagagct acaataacaa
     9241 ggttttaatc aacgcgagtc gttgaaatat gaaatgagga gagtaaaaaa tggcaataaa
     9301 tcggtatgta aaaaaagctg gaatgcttgt tggaatgtta ggaatcattg caagctgctt
     9361 tcaggggtat caagtacacg cagaagatgt cacgcaaaaa acaccaccag agaaggtcaa
     9421 tatcacggtt cataaattaa tgtacgatca aggaacgcaa ttgaatgttg atattgacgg
     9481 gatcaaaaac gatggttata cgcacgatca atatcctaca ggtgtaacga aatacaacaa
     9541 ggcagattat ggtgatgttg agtttacgtt aggaaatatt acggatcaag tattaccgag
     9601 agaagattct gatctaacca acgcgaaagt ggacgagatt gtacaagatg tagaagacaa
     9661 gggatctaac agtgagtacg tcaagaatgc aaccaacaaa attacttcag ctgtagatga
     9721 aaatggtgag atcactttta ctgatcaacc agcctatgtc aattccaaag ggaatgtata
     9781 tgtggtttat gaaagtaaat ccgctgctgg attagtcaca caaaaagcaa aaccaatggt
     9841 tgtgattgcg ccaatgacag ataaaacagg atcaggattt ttaaaaaata ttcatttgta
     9901 tcctaagaac attgtttcta agttgagttt tgaattaacg aaatttggtg acgatggcgc
     9961 agcccaaagc aaacaaaccc ttttaaaagg ggcaaaattt gagttataca agggcgaacc
    10021 tggaaaagga acaaaattgg gtgatcttgt atcggatgat caaggaaagc tgactgcgac
    10081 tgatttgact ttaggaaaat actattttgt ggaagttcct tcggaagttg tagtgggatc
    10141 agatcaagaa ccaacagcag atcaatattt attaggcgca gacgctcgca acgatgcgca
    10201 taataaatta acctttgaaa ttaccaatga cggcgtgaca agtgacttaa aagcttccta
    10261 cgtgaactat aaagcaccag tgatcgataa aacagtgaca aatggaacag gacaagaaca
    10321 ttctttccaa attggtgacg cagtgaatta tcaaggaacg attcatatcc ctacagatat
    10381 tgcaggtgga acagatggga ttacagtaaa tggtgtgaag agtgaaactt ctccttacag
    10441 cgtatttaag tggggagata cagcaggaca aggactttct tatgtcgctg ctaaagcaaa
    10501 tatcaaagta acaaataaag atggatctgt ggtattaaaa gaaaatacag attataaaat
    10561 ccaaaattca gaaaatggct ttgtgatcga ttttatcgtg aataatggcc aagtaagtga
    10621 tactgtagca agtttacacg gacaagatct tcaaatgact tataacatgt atgtaaatga
    10681 cagcgcagct gttgccaatc cattaacaaa tagcgtggat ttcgtttata acaacaatcc
    10741 atttaatcag gaagaacacc acgaaaaaac gaaagctgac gttgtgactt atggtgcaaa
    10801 attcttaaaa gtcgatagcg gactatttgg aacaggaatc aaagcaacac cgcttgaagg
    10861 cgcagaattt gctgctaaaa acgcagaagg aaaatattat ggtggattag ttgatacgga
    10921 taaagacggt gtcaaagaag cagtatgggt agacgatgta gcaaatgcag cgatcctaaa
    10981 atcagataaa gaaggtcact ttgaaatcac tggtttaact gaaggtgaat attcactgga
    11041 agaaacaaaa gcaccaactg gttaccaaaa attaacagaa gatattgcat ttaaagtaaa
    11101 caaaaactca tttaaagagg aaaatcgaat cactgttaaa aataatcaaa aagcagctat
    11161 tccattaaca ggtagtaatg gtttccaaac gtatgtattg atctcttgct tgttgctagg
    11221 agcaggggca ctttctgcag ttgtttactt taagaagaaa gcctagaagg atcagctaat
    11281 gattattcgt aagcccccaa aaagaagagt catgcacaaa ctgatgatat ttttttggtt
    11341 gctgtcggca gtagcagtct atttaattcc agctgtggaa acaaatgtag cgaaatttca
    11401 acaacagcaa agaattttag ccattgaaaa tcttgaaaaa acagattctt ctttggatgt
    11461 tgcgaaaata gagttaggcg atcctgttgg tgttcttaca attccaagta ttcctttaaa
    11521 actaccaatt tatgacggaa cttccgacaa aattttagag aatggtgtag ggattacgga
    11581 aggaactggc gatattacag gaggaaacgg taaaaatcct ttaatcgcgg ggcacagtgg
    11641 actttataaa gataatcttt tcgatgattt gccttcagta aaaaaagggc aaaaattctt
    11701 tattaaggta aatgaagaac aacatgccta tcaaatttat cgaattgaag aagttaaagc
    11761 agaagaatta caaaaaaatt atttagatta tctcatgcct gaaaaagacc aagataggat
    11821 cacgttaatg acatgcacgc caaaaggtgt taactcacac cgctttttag tgtatggtga
    11881 acgagtgtca ttcagtaaag aagatttaaa actacagaaa aaagcaaaaa aataggaaat
    11941 caatttcatt ggattggcga ttagcactgc tattcgcttt ttttgcgttc ctattatttt
    12001 tatttataaa aaaataagga ggagatcgga atgaataaga gaaaatggct gtttgtttgt
    12061 gtagcagctc tctttgcaac tgcagcttac tcacagatat cttttgctga tagcagtact
    12121 actccaacta ccagtcagac aacagatagt tcaacaactg caacaagtga cacggacagt
    12181 tcgaagaaca ctgcagaaga aacggcagat tcaagtacga gttctactga atcagcggaa
    12241 tcaacgtcag aaagttcttc agagacttca gaaaaactgc aagaagtaga agatacgcaa
    12301 gtaatcaagc aaaacattca tattcaaaaa attatttctt cgaatcgaat caacaatcaa
    12361 ggaaggcaag tgactcagca agaaggtgtc aatggtgcaa agtttattgt ctacgatatc
    12421 acgaatatct tagaagaaat gacacaagat gataatgaga aaaatcaatc ggtagaaata
    12481 attgagtcaa aattgaaaga tcgagcaaaa aaactttcat acgatcagtt gaaaaaagtg
    12541 actgaaggag aaacccaaac aattgatgat caggaaggcg tgattgattt ttcaattgaa
    12601 gtacctgcaa acaaaaaaca agcatactat atcgtcaatg aatcaagccc tgaaaacatt
    12661 tcaaatagcg aagatattgt tttattgaca cctgtttcag atgaaaaggg tgaatttttg
    12721 acggatgttt ggttatatcc taaatcaaag aaatcagagc cgaaagaaga aattaaaaaa
    12781 gttgtatcaa ctggtgtaaa gaaaaacttt tttgataatt gttgggattt tttcactcat
    12841 ttatttttta acgattagga tgaaatgatg aaaataataa aaatgttagt ttttaatcta
    12901 tcttttttag ccgcaacaat attgtgttgc ggtttagttt tttctcaatt atcaatttac
    12961 ccaagatatc aacgattgtt cgttcatttt ctaaatatcc agcatgcttc catgccacta
    13021 gaagtgatta acaagctctt aatcaatcca atttttatgg ctagtttgtt tcttcttgca
    13081 ggattggtaa cgtgggtatt aggaaaaaaa gaatgagaag cttacaatcg tttaacaaaa
    13141 taatagatgg agggcattat ggaaaataaa attcattgca ctacgggaag agtatttact
    13201 ttagtgttgt ttttgttcct tcttcttttt gcaaggggaa ctgcagtcca ggccttagaa
    13261 aaagttgatc cgagtcaaat caaagaaaat aataatctgg tactttatca tgatagtgat
    13321 tcattgagaa atatcgaagt aactgggatc aatggattta agatactaga tgagtattca
    13381 actaaaaaac aaaaagtact tgcttggcca aaagtcaaag atttgaaaag cacgtcaaaa
    13441 attgttgccc attatcaaaa ggtgggcact tatctaggaa aagaaattga ttgcgacgta
    13501 actttttcta attttgtagc gcacgctggg gttaaccaag acggattagc taaaacaatt
    13561 aatgggaaaa agtattataa ttatttgctc tttgataaaa acatttatcg aggctttttc
    13621 atgcagtcaa tcaacgttat gaatgttaaa ctagatttta agtatgcagg atcaaaaaca
    13681 aaagtagctt tgagtgaacc acagccagaa attaattcaa aaatgagatc gatgagagca
    13741 gctggtattg agttgaatga tacatttcta tctatttaca gtttaaatgg acagtatcag
    13801 caacgtgtgt cttggtataa tgacaataat gaatctgtcg gtgaacgtaa tattgatatg
    13861 agagaatttg ttgggtacga aaaaatggat cgcttacctt attttgtcac aacagataca
    13921 gcatgtgcag aatacaaagc tcctgcgtta tcaacaaaca atttaacttc aaaagtagtg
    13981 ggaggacgtg cagaaaaggc ttatagctcg aatgatcatt tcaccgatgt tgtaggagct
    14041 gatacttatc acagaagtgg tgtaacgtat acgcttcaag gcgcttcccc aacattcatg
    14101 attggcgcaa atacgaatag tatgatgttt agctttgata ctgcattgct ttggacacca
    14161 caaccatcga agcctacaaa agaagtgttt aacaaagcta atactgaaga ggcagcacac
    14221 aatattgaca aaaaagtgat tccacaagga tcagatgttt actatcatat tcaccaaaag
    14281 tttgatgcat taacagtcaa cacaatgaac aaatacaaat catttaaaat cactgatacc
    14341 tttgacagca aaaattttga tatggtatcg gatgggaaaa actatgatgg cgcattgtgg
    14401 tatgttgaag cgaatgggac ttggaaaaaa gctgatacag gaggaaaatt aactgtatca
    14461 ggtaatacag tcacttatac tgcttctgac agctttttag ctaatgctgc aaattacggt
    14521 agggactaca ttttgcagct ccatatgaga gcaaaaaatg atgcaaaagg aattgcacca
    14581 aatgtggcta ctgtaacgtt taacaatgat ccgaacaaag gaacacaaaa aacaaataaa
    14641 gtagaaaact atttctctct taaaccaaat aaaaaagtgg aacaagatgg gaaagatgtt
    14701 gatggtcgaa atgatggtaa aaaaggaacg cctacagctc ctttaaatgc aggatctgaa
    14761 gtacaatatc tagtcacaca aaaatggcat acaaaaggtg tagacattgc gctcgatcat
    14821 tacaagcaat ttagtattca agatccagtg gaagctcgtt tgacgtataa agaaggttct
    14881 gcgcgagtga tagatatgag tacaggaaaa gatattactt cagaaggtat actaacctat
    14941 gatagccatt caagaacgct gaaatgggaa gcttccgctg atttcttaaa taacaatctt
    15001 ttagatggac gagaaattca actgatattt acagctaaaa ctcctttgaa tgaagaattg
    15061 gatatcgaca accaagcaaa agtttcagta gaaagtattg agaaagcaac taatgtagta
    15121 acgattgggg taaaacctaa cttacctcag gtcattgtgc caaaaactgg atcgaatcat
    15181 ttagtgctaa ctgttattgg aagtatatta tttcttactt tagcatcatt gggctatgta
    15241 gttttaaaac tcaaataaat ataaaaacaa ggctgatttt cagccttgtt ttcttttatt
    15301 tgaagattgg attcgcacta ataaatacat tatctacgcc aattctacca gcagagtcaa
    15361 ctacaaaatt atgacctaat ccgagaatac tttggttatt ataaagtaaa tttgcattat
    15421 gggcagggct atttttccat cctaccatgg catcgatagc accatcgtct gataaaaggt
    15481 catcaatata cagcccagtt tgacttatgt tttctccctt agcaccatag cagcctaagc
    15541 tttccaataa atcttggaaa gtaccaccac cagggcgata gtgactgaag ctgacttgta
    15601 attctttagc tcgaatatct gcagcctgtt gaacaatatc attgaattgc attggggcaa
    15661 caccattttg ttggcgataa tcatttaacc aagcaagttc tttttgacgc ataaagttac
    15721 gtaattcgtc taatgaaatc tttttatatt tgaatgtgat tgttttattt tgatcgacgt
    15781 tattaatgga tcgttggccg tttttatcca cggtaacggt aggatcgtca tttctgcaca
    15841 agaaataatt ttgatcagtc atatttttcc aaggagtaga gaagctatcc ccttcgtata
    15901 ctgtttttga ttcactagag agaacagcgt tggtatcagc atttacatga ttaacagtca
    15961 cattaaattt acgagtgtag ttgaaaatat aattttgact ttcagtcaca ttgtttaagg
    16021 ttactgaatt tgttggttct acccgatcgt ctaaaacata tccttgaata gaaggagcag
    16081 ttaaagtgac actttcaccg tgagtgacgt tgattatctg ttctttcaaa atatcaactc
    16141 gcccctcatt tttgtagttg ttattgcaca acaaattgat ttgatgcact tgattataat
    16201 tgaatgtgat tgttgtatta cctgtgattg ttttgctttg agtatcatta ccaatcactt
    16261 tgtagctaaa attatcttta gcaccttcgc tatatttaaa tgcttttgcc ttagcagtgt
    16321 aagtagaacc agaagtcact tttgcttgat ctgtagcaat cacttgacct gtttgattgt
    16381 caatatggtt aatggtaacc atatattctt ttggcgtttc aggtttgtgt tgcaaccctt
    16441 taaatgcagc atcaagacta tttttagcat tgtttacttg tgtttgagaa accttagaat
    16501 cgtctagtac ttttttagca ttggataaag cagtctgaaa attattccaa gattcttctg
    16561 taaaatctcc tttagcagtt gattttactc ggttgtataa agcttgtaat tcagctttat
    16621 tcacagtagg aactggtttt ttctttaatc ctttgtaagc agaatctaat ttatttttgg
    16681 cactatccac ttgcttttga gtcgcttttg agtcatttaa cactttctta gcattgttta
    16741 aagcggtttg gaatgcgttc caggtattgt cggtatagtc accttttttc gttcccttaa
    16801 cgctcgtata aagagcttct agagccgatt tattcacttt gggtttagag gtgtcagcgc
    16861 cataccaacc gataccttcg tatttccagc catagtcttt ggttaaccat tgtacttctt
    16921 tccaacttgc agtgtagtga tgtgagccag attttacacc aggaaggaaa aggcggtaga
    16981 tcggtttatt atttttttcg gcagaacgcc aggcaggccc ttcataagtc cagccgtaat
    17041 ttttagtcaa ccatttcact tcatcccaac tagctgtgta atggtgatca cgaagacctt
    17101 tattgtacaa acgatagaca agatggccac cgctagtcgg tgcttcccag gcggggcctt
    17161 cataatttcc ccattttatc tttactagat ggtcacgttc ttttttgcta gaggtgtaaa
    17221 aatgttcatt attatttgga ttgtaaaggc gatacatgac gttagttgaa gcagcgtcaa
    17281 cagatccagc aagactaagt gaggcagcaa atgttccaat agctaaaaca gtttttggat
    17341 taaataattt cattttttca actcctaaat ttaattaaaa atgtttacat gattattata
    17401 tatcggttaa ccgatatagt caataaaaaa taagaaaagc cttatataat tttggctttt
    17461 cttattttat ttgttagttg aagtattaat atcaacgatt tcttcagcca atttatttaa
    17521 tttcatagca tttatgaatc tcatgttgtc tatactagag cgtttgttgg caaggtcact
    17581 tactgtagtt aagggtacgc cagattctat ggatatagaa cgaactgatt taccactatc
    17641 aaacagcaac cattcgattt tctttttatc agcgtacatt tcaatgctcc ttcttgtttt
    17701 ttctttatta tagctctttt ttgtaatgaa tcacaatatc aagagaaaaa ttttctatag
    17761 aaaggtggat ttttatgagt attcgttctc cgtgtatcca aatgaggttt gttgctactt
    17821 tcgttatttg atatactaaa aataaacaac gaaagaagga ataaaatgaa gaaaaagcaa
    17881 ctatggtttg tctctatgtt tgcaatattt tttattattt taagtggatg ttcaagtaat
    17941 aagaaatcgg aagctaaaga agataatagt ttattgcaac caggaactgt ttggaaagaa
    18001 gaagtaggtg gtcttattca taatttaaaa attatagatg atactacctg ggaatattct
    18061 gaaagtgtat ggcatccaga tcctgtacaa ataacagtta agaggcaaaa agactatgaa
    18121 ggtttagaaa gatacaaaat agtagattct gcaggcatta gggaatttat aaacaaacct
    18181 gattcattat ttattgttgt tccctatgag aaggatggcg taaaaaaaat aatttttctt
    18241 ggatctagca aggatgagaa gcgaacgaaa gagaaattaa ttcatgatgg atcacaatca
    18301 aataaataca aattacaaaa gactagcgag tgattgctag tctttttatt tgtcctgaag
    18361 gaggaatcac agtggcaaaa gctaaaacgc catttgaaaa agccgcgtct aaaatcttag
    18421 aacgccgcgg tgtatctgaa acacagtgga aacaagaagt ccttgaaaaa gcacaatttg
    18481 acctattacg gggaaaagac aaggacttag aaggatatat ctatcaacgt gaacgtgaaa
    18541 agcttgtttt cgatgaaatt attaaacact aaaaggagcg aacaatttta tgtttgaatc
    18601 aattacaatg aattttttaa cacaaccgaa agtagtagct gcggttgata tttttagagc
    18661 attacaaact gccgcaacta gtttgaaaaa taatctgatg atttttgctg ctgttgcggc
    18721 agtggcttcc attgctcttg gaggtatttt cttcttcttt gggcgaagag gagctgaaaa
    18781 cgggaagaca cacgtaggtt atgtattgtt gggggtagga gttattttag ctgccgcttc
    18841 tattgtagtt tcaatgttct caatgtttgg acaaacccca caaggactat aaaaaaacaa
    18901 gtagaaggag taaaaagtac gtggctactt tttactcctt tttttatgga aggagaatat
    18961 tcatgttttt tacgatttca ggcaatgcaa aattggataa agagttaata agaaaagcaa
    19021 cgaagaaagt gggaaagcgt acttcctttt taccgaaaac aattaaatat tctgtcattg
    19081 aaacggaaga acagctacat gtgttaggac aagtgttaga acaagagaat caatcttctg
    19141 ttaaattgac tctcacgtta catgataaag aaggaaaaat agaaaaggct ggcacagaat
    19201 acgtttttgg ctcactggat tatcaaattt cagaaggttt ttatcctaca ttgattgatt
    19261 taatccgcct agatatattt aaagatacgg atgaaaccat gagtgtggaa gagtcttatc
    19321 gaaacgaaaa aattttacaa gttttagaag aagagttaga aagcgaccaa agaaaagtga
    19381 atcaagtgag tgaagtcgaa gcaaacgaag aagtaccatg ggaacgccca atctttccaa
    19441 aggatgaatc aaaagaaaaa gagaaaggaa ttattgacga acaagctgac attttgactc
    19501 ataaatcaat atttgaggaa gagaacgagg taatgggtca attggaggta gatccctttg
    19561 aagaaacaat ggaggatgaa gagacggccg aagtagaagt tgatccagtt gaagctaatg
    19621 aagacccaac atggactgaa ggggattctc aggaatatat tgaaccactt atcagtgaag
    19681 aagtagagaa aaatgaaaca gagatacaaa aggcaacggt atttgattat attttggatc
    19741 aatatccgcc agagcatgaa tggatgaaag aaaaaaataa attattttta aatgaacttt
    19801 attcgaaata tcaattacct caaacctatc aagcattttt agagagtaaa gaagaattaa
    19861 tctcacaggg taaaggcaca cttttagaac agttagagtc tgtccaatca acaaattggc
    19921 atgagcttgc acaagaagat ttattagcta cttttgaaga acgtcaacaa gaaacagatc
    19981 aagaaattaa tgagtatgcg acggaacagt cgtcgaattg ggagaaagca aaagaggaac
    20041 taaataaaga agaacagcga gtgattgaag aagaagtaca aaaaattaaa gagcggtacg
    20101 aaaagaaacg tgacaatgcg tattccttgt gtgtagaaaa gatcaacaat tttactcaga
    20161 cgaatcaaga agcattatcg aaagaaaaag aaaacttatt aaaagaacgt ataaaagaac
    20221 gaaaagaaga attttaccga gggttgcgta ctgataagct aaggattagc aaagaattaa
    20281 atgaacaact gaataaactc tatcaaacta cgactgaagt tctgatggaa aaacacgagc
    20341 agatccaaca agaattacaa gcacaaattc caaaatggaa aaaagagtat gaaattcacc
    20401 aacaagaatt gaaagaacaa gaagaaaaac aacggaaaaa agaaaaagag cgcgaacaat
    20461 tagaactaaa aaaacgtgaa caggatcttg cggaacaaaa aatggcgata gaaaaacaac
    20521 ggctagaaca agaaaaggaa aaagaagcag ctgctcgtaa ggaacgtcaa gaacaaatgg
    20581 atacattgcg acaagcacag ttaaattatc tgacacatcc tcaaccagta gctgctgttc
    20641 aaacgccagt gaacaaccaa gcaaatttaa ctccttcacc ccagcgttcc tttaacggat
    20701 ggatggtggg atgtattgca agtttaagct tgcttctagg tggaggaaca gtatttgctt
    20761 ttaatcatat caatcaatct tcacaggctg attctgtagc cacaacggcg tcaatcaagg
    20821 aagaagccca ggaacaagca agaaaagagt atgaacaaca attagaagaa ttattgaatc
    20881 gtaccaatca gtcaacaaca gagtcaagca tggaaaatag caaagagagt gattctagta
    20941 aggctaaaga aggatcaagc gattcaactg tacaatcaaa cacttccgaa agttcagaat
    21001 caaatgaggt gaaataatgc gtgttagaga aacaacgaag cgaaaagaca aacgattcaa
    21061 tttagaaaag aataggtatc tttctcattt ctatcgctat actccctcag gagaagagct
    21121 accagaaaaa catgacaaac gcatttcgaa aaagattttt tatagtggac ttgtatacct
    21181 ccctttagag tatttagtaa caagagtacc agaagttttc aaccgattgg aaattgttaa
    21241 gggaagctta caaggaataa atctgtttca attacaggaa atgccacagg tctttcattt
    21301 tgattcagct ggtttttgga atccatttac tcctacaact agtttgggta cacgtttagc
    21361 aatttcgaca gtttgtgtgg ttccagctgt acttatggga ttgaaaatgg acatgtggtt
    21421 tcgtcctcta gcagatggac aaaaagggga taatcgatta atgaccttaa aagaaatcca
    21481 acagatttat ccgcaaatac ctgaaacaaa aactactttt ccaggttatg gaggtattcc
    21541 aatcacacat tacaaaaaat attggtacat tcaaacagat actgttaata cgtgtattgt
    21601 tggtacctct cgatcaggga aaggtcagat tgaagtgtta gccaccattg acaatttatc
    21661 tcgtgcagaa aaacaaagtt caatggtcgt taatgatcca aaaacagaat tatatgttgg
    21721 tagtaaagat gaactagaag ctagagggta tgatgtatat gcctttaata tcaccgatcc
    21781 actgcaatca atgagtgata atccattggc actcatcgtg aaatactgga aacgaggaga
    21841 catagatact gcaacacagt taaccaatac tttcacaaat acgatttatt atgatgccaa
    21901 cgcttcagat aacaaatact ttaacgaaaa cgcgcagaaa gctgtcaatg cgcttatttt
    21961 tgcgttgtta gaacaagctg atcaaacagg agattactcc aaattgacac cgaataatct
    22021 cgttgagctt ctttctgaga ttggcggttt taattattat ccagatccag acaatgaatt
    22081 caaacagttg aatgccttag atgaatttat gaatcagttg ccacctggaa acattgcaaa
    22141 aaaacaatat ggggcaacga aaattggatc tgataaagcg aagggaaata ttttatcaac
    22201 ggcaatcacg ggattgaatc catttactct aacgaaaatt gcaaagatga cttctcaatc
    22261 aacgattgat tataaatcgg ttgggtttcc aaaatactta gatttgaaat tacatgaaag
    22321 tcttttaaat caacgagtcg tagtagaatt ttatcataag ggaaagaaat ttcatgaaga
    22381 acgtgtcaaa gtaggctata aaggatttag tgaaatcaat tttgattgtc atttagttgc
    22441 aggtgatcaa gtttgtttaa aatacgatca tcaaggaaaa gaatatgtgg cttggtatga
    22501 attttctcaa agagtattga aagacgaaga gggtaatatc gtttatcgga aaaaacgagg
    22561 agaaacccat ttgccagaat tagaaaaaga agccgttcta acattagatg aggaaaaaag
    22621 taacttatta gtagagtcta ttcggatgca ctataccgat aaaccaatgg ctatttttat
    22681 gcttacacct gattatgatc catctaacca tatcattgtt agtatttttc ttagtcagtt
    22741 atataaagaa ttatctacgc aatgtgttaa gactaaagga gataaatgtc accgcaggat
    22801 tcattttatt ttagatgaat ttgggaatag tgtgacctgt ttagcctaag gataaaagag
    22861 gctagttcca acgaacattg gaataacttt actatcgaga aatcaaatga cggggtaacg
    22921 ccctgaaggg tttccactga tactgcgaca tgcgttagta ggatgtgcat ccaaacgcgt
    22981 atgaagctcg ctgaagtcgg cagaagggta ccgtaaggaa agacacgata cgtatctacc
    23041 acgcaagtgc tctagaggta gggtatgtac ctgatatgtc gggtgtccta aaaagttcaa
    23101 taaggtgaga atgatttgat tatctgacaa acttgcgaat ggaatgtctg aaacgaataa
    23161 ctcatagaaa tgtgaggggg aagggggata cgtcctcttt ccattgactc gaggttgagt
    23221 aaaaatgtgt ttctatgaaa aaccttgcat ggttaaaggc tatggagtag tcaacaggca
    23281 aaagcaagca cctaagttga aatctatgga tagatagtaa ggaacagggg aagcatctag
    23341 cacggaggga gcataccgaa gatgtgtttc taaggaaaaa ccgaaatcgg ttataactta
    23401 ggtgaataga tgtgaaagta gagccacgac cagtgaaaat ttcgtaatga aattggagga
    23461 acaggctcaa gtcgttattt ataatataag aaacctattt aaaatcatgg attctcgtaa
    23521 gactaaaaag gtcacgaacc agaatggagc gtgataaaac catgactaac acaaaaggaa
    23581 ttagcttaaa gaacaaacaa ctcagacacg ccgaatacta cgacatgact gagactttcg
    23641 atgaattgca cgcaatgagt gttgcaaaat caaattttaa aaatctcatg aaattcatta
    23701 ttgatgacaa taatatcttt ctagcgtata gaaatatcaa aaagaataaa ggaagtgcaa
    23761 cggccggata cgatggtgtc actataaaag acattgaaaa attggcatgt ggtgactttt
    23821 taactattat taaaaggcga tttaaggatt acagaccaag aaaagttaaa agagtagaga
    23881 tcccgaaacc aaacggaaaa cttcgacctt taggaattcc ttcaatatgg gacaggatag
    23941 tccagcaatg tattctacaa attttagaac caatatgcga agcaaaattt aataagcata
    24001 gctatggatt cagaccaaat tgttctacag aaaacgccat agcagattgt cttgctagaa
    24061 tcaatcaaag taagatgaat tatgttgtag atattgatat aaaaggattc tttgacgaag
    24121 taaaccatac caaacttatg cgtcaaatat ggacaatggg gattcgagat aaacaactcc
    24181 ttgtaattat tcgtaagatg ttaaaggctc cgattgttat gcgaaatggc aatgttgttt
    24241 ttccaaccaa gggtactcca caaggaggga ttttaagtcc actgcttgcg aatatatgcc
    24301 tgaatgaatt tgattggtgg atagctaatc aatgggagga acgaactgtc aaagagctga
    24361 aacaccacta tcgaaaaaac ggagcatggg ataaaggaaa tatctattcg aaattaagaa
    24421 gccacacaag tttaaaagag atgtatctgg tacgctatgc agatgatttt aaaattttct
    24481 gccgaacagg agaagaagcc caaagggctt tttatgccac acaaaaatgg ctcagagaaa
    24541 gattaaagct cccaatttca atggagaaat ctaaagttac gaacctaaat aaagctgaaa
    24601 gtgaattctt gggtttttca ataagactcc acaaaaaggg aaaaaagaaa gtggctttca
    24661 cgtacgtttg tcgcaaagca gaagaaaaca ttaaaaataa actaaaacaa cagattaaaa
    24721 aaattcaata cgctggagca aaaaaaaata ggcaccgagc aataggtgac tacaacatga
    24781 tggtcatcgg tatccacaat tactatcgga tagcttcaca ttgtagtgtc aatttcactg
    24841 agatttatag aagtgttagg acagtaatag aagcaagact gaaagctaga ggaatgacga
    24901 aaaatggaat ctatggacaa gatcatgaag gataccgtcc gtatctaaag tctaagcaaa
    24961 tgagatatat tgaaggtttt ccgatacttc caattggcta tattaaaaaa agaactccac
    25021 tcaacaagcg gagaaatcta aataaatata ccaaggaagg gagaaagctc attcatcaag
    25081 agcaacaagc tatagaaagc tggaaaatca agtatcttgt aaaaggtttt agtaacaata
    25141 agaaatacac ggtggaattt tctgataaca aaatatctaa gtttatttcc caaaaaggga
    25201 aatgcgctat cacaggaaac gaacttttga tggaagaaat tgaatgccat cacattaacc
    25261 catatcattt gtccaaagac gatagttaca agaaccttgt cattatctct agagaaatcc
    25321 atagattaat ccatgcaact agtgaagaaa ctattctgaa actgcttcgg ttactgaaac
    25381 taaatcacta tcaaattaga aaactaaaca cgtatcgaaa aaaagcagct aattttccaa
    25441 tatcaatcaa aatgaattag ataggaaaca aattatattg taattaacga tggaacgccg
    25501 tgtgcggtga aagtcgcacg cacggtgtgg accgggggaa aagacggaga taatatcaaa
    25561 ctcttaccta tcggaattgc caccaatgga taatatggaa ggaatcatga cggtcacagc
    25621 tggccgaaac atgttgtggg atctgtttat tcaatcttat cagcaacttt atgcgaagta
    25681 tggagaacaa aacgcagcga ttatcaaaga aaattgtcag acacatattt atattatgtc
    25741 gacagatgat aataccatcg aagaattttc taaaaaagtg gggaataaga cggttgaaca
    25801 agagaacagt accatcaatt cattaggtat gaatactcat atcaatcgca atgttgattc
    25861 agatcgtatc ttaactcctg aaagaatttc tactttttta gaaggtgaaa cgattgtact
    25921 cgctccttta aaacgacaag atttgaaagg tcataaaata cggccgtatc ctatttttaa
    25981 tcatggggaa acgaatcttc cttatgctta tcaatatatt gaagatttca atccatcgtg
    26041 ggatatcaat gatatagaca tacattgtac acatgcggac ttggatctta aggctttaca
    26101 aattgattat gattatttcc taccagatga atatgctaga aatctctatc atggactgca
    26161 tgatcaatta gaagaaaaaa acgaagaaaa tatgttttat aaaatcgttc aatctcttga
    26221 gaatgagacg gctaaaaaat tgttaattca attttacgaa gaacaagcag cagatcgact
    26281 tcaagaatat gttgaaaagt tgatcaaaaa tgaagaaatt tctgaaatga ttgggaaacg
    26341 gttgttattg gaatctgaaa aggtggtgaa agaatgatca ttggaggaat tttttctggc
    26401 atagccgact ggtggtcagg cttattcgga gaaggggatg caaaccccaa aaccgtaacg
    26461 aaagtcttac aagattatgg taattatctg cagtatcaag atgtgttcag ttggatttgt
    26521 aatacttttt tatgggggtt gatcaaaggg ttatataatc tcaatacttt actagaaaaa
    26581 accatttatc aaagctttga tttaaaagac ttattgaacg ccgctggggt aaatgaacta
    26641 tttcaaagtt tgattagtaa ggtattggct cttttttgtg tcgtcacatt aatttattta
    26701 ggcttgaaat tttttatgag taaacaccca ccaaaagtca aaaatatctt ggttaattta
    26761 tttatggcca tgatcttgat ttttggtggt agttctttaa tcgatgaagg gttaaatatc
    26821 agtaaaagct tttacggaga cgtcactgca gcaaataaag cagataaaac gggttcacct
    26881 gcttttcaaa ttattcaaaa taatgtgatc gatgtcagta cattactagc aactgacccg
    26941 aataaagtag aatccctgcc aacagacaaa agaaatgctt taacagcgaa aaattttaaa
    27001 acagcgaata tcaatgggat tattactcct aaacaagcta aagaaatggc ggataaagat
    27061 aagagtggtt tatcagattt acaaaaggat cggttaagag cgttacaata ccgactggaa
    27121 ctggatgata atggaaaaga aattcctgtg caaatcagtg atgaaggctt agccaaatat
    27181 gtttatactt cagggtatcg tcgatacatt agtagtccag ggattatttt agctggtgaa
    27241 tcttctttgg cggtggccta tttatttatc ctatttacga tcgttacttg cattattgag
    27301 ctggtattta aaaaattcta tttagtgatt gccgcgtcaa cggactttga gacaggacaa
    27361 cggatgaaaa gtgctatcga agatatttct caatccttct tgttattggc atttacaatt
    27421 ttagaattac gaatctatac cttaatgctt tcaggtattg gtgatttgca tgcggcagga
    27481 aagatcaatg gattcttgta tgttgtcgct ttgattgtac taaccattgc gctattcaaa
    27541 ggctctcaat cagttacgaa aattttcggc gttgatacga gtctgaaaaa cggcagtaat
    27601 tctcttctta gcatgtttgc cttaggaaat attgcgaaaa atgtgggtag cggagctaaa
    27661 aaccttgcaa gtgctggtaa gagtggttta agccatttaa atgatattcg taaaaatgga
    27721 tttggccatc gaaaagatgt tacccctgat aacggggaag aacaatcaga cgataaagct
    27781 acaactgtac ctaatagtaa gaaaacagtt tccgaaatgt tcgggaaagc gaaaaatggt
    27841 gtttctaaag ccgcagaagg tcttggttac gtcaatgaac gtgggttaaa aggaacagct
    27901 gaagatctta cagaaaaagc caaagacacc ttagaagata aattagacgg cacgagagct
    27961 aaagaattgg ctgaagctgt gaagaatcca tccgaagcac tagaaaaaca aaaagaaaaa
    28021 gcttcagaag tcaaagaagg actaaaagat gcgtatggtg aaggacaagt caatgcagca
    28081 gtgaaagcta atacaacaac cgaaaaagaa aatcaagaag gtatagtgat tccttcagtc
    28141 aaagatcacc aagaagcgtt gcactctaat gaaaattcga ttgaagagaa aaattccata
    28201 gaagaaagaa attcagaaga aggaaaaaat ggacagttac caaaaactga tccagaacaa
    28261 tatcgctata aacctaacag tgaccttgaa aatgagaagc gaatttcaga tttatcaaaa
    28321 gtaaatcaag aagacaaagc agaaaatgag ggacagttac caaaaactga tccagaacaa
    28381 tatcggtata gatctgttaa ctccgaaaat gaaaaacgca tttcagattt accaaaacga
    28441 aatcaagaag tacaaacgga tgaggattcc atcgttctgc caaatgataa tggaatcgaa
    28501 gttggaactg aaaaatcaac gaaaattggt ttacctaagg cacaaaattt accaatgaaa
    28561 gatagtcgtt tgaatcaatt caaggataat gatcaaagtt ttgttcatca atcacctgca
    28621 ggtactgttc aaatgaaacc aagtcgtttg cctgaaaatg gaaatctgac aactgtttct
    28681 tacaatttat ctgaaagtgg tgagacaggg aatgcgtccg ttcaaaatgt tgtgacaaga
    28741 gaagctgcac caaaacctat tcaaaacgtt catacaggtt acaagccagc aaatgtggca
    28801 tttactgcac caacaaaaat aaatgtgaca agagaaacta aaaacaggaa caatgcattg
    28861 aaagaacaac tattgaggga tatcaaacag gatatcaaac cataaaaggc ctacaagtta
    28921 ggccttttct ttatatgaaa aggagaaatc aattatggat aaatatataa acatgaaaaa
    28981 ccctccaaga tttagtggag aatggaagtt tttcgctttt ttctttttag atctattaac
    29041 gcttggtggc tgcttttttt gggcgaaaat ggtcaataat cttgtccact taccactctt
    29101 ttttgcactc ttgaattatg gcttatggat tgcgatagcg ttagtgattg tttggcgacc
    29161 ctataataac ccaggtcaac gacaaatttt tgcgattatc gagtctattt tttttgcgga
    29221 caagaaccat tatcatgcgt tagatcctaa ccgaaattat catggtacgt ataaggagga
    29281 tgaataaatg cgagtcaaag gacaaaattc agttgaaagg aaatccattt tgcagtttgg
    29341 aaaaccagaa gagcgctcta tcttggactt atttccgtat aaatcctgta cagaagaggg
    29401 agtcttaatt actagagaag atcattttca acgttattat cgtgtagttt ctacagacgt
    29461 ggaaggatta aatgaacaag agaaactaga acgaatgaat cagttgacca ccgtcatgcg
    29521 tacgtatgtg ccaacaatta agttagttag tttgaccaca gagacggatc taacggaaca
    29581 aatcgtgcaa aagcggcagt tattagataa aaatcgtatg gaacagtcct taggaagaaa
    29641 tctacagcga ttaagaaaat atgaaaagaa attagtagag gaaatccagg aattgaaacg
    29701 agcagaacag gaacgccctg atttaagctt ctttttcgtc attgaaggaa agacaatcaa
    29761 ggatttgcaa accagaaacc gtcagttaat gcgatctagt ggcattttag ggttaaaacc
    29821 actctacaaa aaagaattga tcaagattct ttatcgaatg aataatatga atgatgagta
    29881 ggaatgggga agagagaatg aaatatccgt tgatatggtt agaaaaagat gttccgaaag
    29941 aagagaccac tagaaaattt cctcacactt taagcctggg taagttggga aaaggaaaat
    30001 cttttaacca agtaaagaat caaacaagaa gaggtgactg tacatgttag aagcaattaa
    30061 aacagctttt tcaccacaaa atgatcaaaa ggtacttgat ccattaaagc aactaggaaa
    30121 tcgggaaata gaaattttaa aatccaaagg gtatgattta gactttttaa aacaagtgat
    30181 gccacaaggt ggattaaatt tcgatgaaga gtatgtgacc tatggaaatg gttattcacg
    30241 atgtgtgaca gtttatcact acacaaaaaa tcctacgctt ttttggttgg ccaatctcat
    30301 gaacaatcct catacagtgg caacactaga tgttagcaca gatgaaaagc aaaaagtgat
    30361 tgatcaaatc aatcgtgcct tagatgaatt aaatgatcgg tcagataatg aacgaaaagc
    30421 gactgaccga aataaaagcg cggaagaata cctagaacta gcggaatatg cccgaaaatt
    30481 gacttctaat ggggaaattt ctaaacaagt caaagtgaga gtacatgttt acggaaacac
    30541 gattcaagaa gtagatgaac gagcacagga actgatcgaa aatctcaaag ggatggatta
    30601 tcgtgcagtg acctatctgc acaaaacgaa aagagactgg caatcgatgt tcaccatgtt
    30661 cgatgaacaa gctgaatggt ttggaaatat caaagggcaa tcactaccat cgctaaacat
    30721 tggcggaggt ttcccgttcc accaccaaac gctaaaagat ccaggtggaa cacaatatgg
    30781 ggaaaccatg acaggcggcc cgtttgtttg gaatgccacg tataaaaata gtgtgcggtt
    30841 gtctaccaat accttgcttc taggaatgat gggagctggt aaatcgaccg ttcttaaaat
    30901 gatcgcggaa gcccatttag ctgcaggaga ctttatttgg ggctttgaaa agggaaaaga
    30961 ctttattccc tttttaaagg aatataacgg cattatggtg cgtttagacg gatctgacgg
    31021 gatgatcaat cctttagaaa tatttgccac acgtacgtat gatgaagctt ccagtttata
    31081 tgacgatgga tcagtcaaag atttgaagat caatgaagca gcgtcctatc aaacgcactt
    31141 agacaaagtg gtctatcagg tacagttagt gagtcctcag ctaaaaggaa cgatgaaagc
    31201 agaatttaag acctatttaa atcgttttta tgaagagtat ggaacagtac cacgcggctt
    31261 tacttcctca aattcgcgtt caaatactga tacgcaagtc acaggtaaag atccagaagc
    31321 ttatccgacg ttcaaagaat ttttagactt tttaggacaa ctggaattac caggtgcttc
    31381 ccaagagaaa aagaatcgaa aagaagaaat ggaatccatc gtagaatcct tatgtgagac
    31441 gtatggcatg atttttgatg gtcatagtac gattcgacac cttgaccaac aacagttggt
    31501 cttttttgac attgactcta ttacaggctt agatcgaggg gtacaacaat gtcaaatgta
    31561 catggctatg acgttgattt ggaatcaggc attgattcaa ggaagaagac aacgacgttt
    31621 aattaaagaa aaacaaatgg aagaacaaga cttcaaatgc ttcttggcga tgattgatga
    31681 gtgccataat ggtgcgacct gttctgcagt gaaaagctgt gggttgcaag tagtctaaaa
    31741 actattgtaa taactcttat aacgagaagt caaatgagtg ggtaacgcca caaagggctt
    31801 ccactgatac tccgacatgc aacaagtagt ttatgcagac caacgttgtg tgaagctcgg
    31861 tgaagtcggc tgaaggatac cgtaaggtaa aagaaatagt gaataccacg aaagttctct
    31921 agaggtaaag ggtgtatccg ataagtcggg tgtccaaaaa aacttaatat ggtgagtatg
    31981 tacaaacgta ctgacgaact agcgaatgta ctgtccgaaa ggcatagctc ctagaaatgg
    32041 gagagacagt gagcaaaaaa tcactcattg tgctaacttg tggttgagta aaagtcgttg
    32101 ttatgaaaga ccatgctgcg ttacaggcgt aggagaagtt agggggcaaa agctagcatc
    32161 taagttaagg catgtggata gatataaggg aacaggggaa gccatcaaca tggagagtgc
    32221 atactcgatg aagtggtagt aagaaatatg tgaatgacaa ataacttact ttagtgatgg
    32281 tgaaagtaga gtcacgactg atgaaggttc tgtaatggaa ctggaggaac aggctcgatt
    32341 cgtctgtaaa agtcatgaaa cccgttcaac catgggattc tcgtatgact aaaaaggtca
    32401 cgagccaatt ggagcgtgat aaaccatgac aaaagaaaca agtccaaaag ataggaagtt
    32461 gcggcatgca gaatattatg gcatgacgga aatcttcgat aagatatact ccaaaagttt
    32521 gaagggagat aatttcaaga atctaatgca gattatcatt tctgataaca acattgtcct
    32581 agcatatcgt aatatcaaga gaaatcacgg tagtgtgaca agaggagaag atggagtaac
    32641 gattaaagat attgaaaatc tcaaccgtaa tgaattcgta gaaattgtta ggaagcgatt
    32701 cgcacattat aaccctcgga aagtaaggcg tgtagaaatt cctaaatata atggtaaatt
    32761 aagaccgttg ggtattccat caatatggga tagaatcgcg cagcaatgca tcttacaagt
    32821 attagaacca atatgtgaag caaaattcaa taatcacagc tatggtttcc gcccaaatag
    32881 atcagcagaa cacgcaatag cagattgtat ggcccgaatg aatcaaagtc acatggaata
    32941 tgtggtagat gtagatatca aaggtttctt cgacgaagtc aatcatacta aactcatgag
    33001 acaaatctgg tctatgggta tacgagacaa acagcttctc ttaatcatta ggaaaatgtt
    33061 aagagcaccc gtagtacttc caaatgggaa gttgatatac ccaacaaaag gaacaccaca
    33121 aggcggaata cttagcccat tacttgcgaa catcaatctc aacgaatttg actggtggat
    33181 agcaaatcag tgggaggaaa aagatgccaa agagctaaaa catcatttta ggaaaaatgg
    33241 agcttgggat aaaggtaata tctatcacaa gctcagaaag agtacaactc taaaagaaat
    33301 atacctagtg agatacgcag atgattttaa aattttctgt agaaattata atgaagcgaa
    33361 aaaagttttc tatgcaacta aaatgtggtt acaggagcgt ctaaagttac caatatctga
    33421 agaaaagtca aaaatcacaa atctttcaaa agaagaaagt gaatttttag gctttacgat
    33481 gaaattagtg accaaaggta acaaaaaggt tgttaatacg catataggta caaaagcaca
    33541 aagcatgatt aaagtgaaac taaaaaaaca aatcaaacga attcaaaaaa gtcctaatag
    33601 caaagatacc tttatctata ttggagtcta taacagtatg gttattggga ttcataatta
    33661 cttcaaaatt gcgactcatt gtagtgtagc aatgcgaaaa attgcaagag aactcagacc
    33721 aagttttgaa atacgactac gagaacaagg aatctcaaaa gaaggacaat acggaaagta
    33781 cgaaggatac aagccttacc tacggtcagg acaaatacga tatataagag ggtttccaat
    33841 attaccagta gggtatattc aacacagaca tccgatgtgt aaaaaaagaa atattaataa
    33901 atacaccgaa gaaggtaggg aagccattca taagaagcaa caaaccgtag aagaatggaa
    33961 gatatcgtgg ttgtgtagca atccagtgct tagtaagaga gcaacgattg aatttgcgga
    34021 caatcgaatt tcaaaattca tcgctcaaaa gggaagatgt gcagtaacag gtgaggagct
    34081 tatcttatct gaaatgcatt gtcatcatat tatcccttat catgaaagca aaagtgatag
    34141 ctatgaaaac ctagtgattg ttactgagga ggttcatcgg gtaatacacg ctacacaaag
    34201 tgaaacgatt gaggaacttc taaaatatct aaaattaaat ccaaaacaaa aagaaaaact
    34261 caatgagttg agattaaaag ttggtaacga agaaatatct taataaatat ttgtaggacg
    34321 ggaaaacaga atgtctttta tagacgaggg aacgccgtat gcggtgaaag tcgcacgtac
    34381 ggtgtggact gggggaaaag acggagataa tatcaaactc ttacctatca gtatcgatta
    34441 atccagattt tattgaaacg gtaaattatg tcacaaactt tcaacgagaa atgcgtaaag
    34501 tagaagcaat gactgtttta gctacacaat ccccacaaga aatggtgcca gaaaatatgt
    34561 cttctgataa cttctcgaaa ctgaagaaag tctttgaatt ttcttctacg aagatcttta
    34621 tgcgtatgga tgaatctatt ttacgtcata tagaaggatt agttggtaaa tcaatgacaa
    34681 actcagaatt agaatccatt cctcaacaaa atcaaggaga cgctgtgatc aattttggct
    34741 caaaagaaac cattcgtgtt cattttagtc cgaacaaacg tcaattaaaa ttgtttgatg
    34801 gaggaaaata atggaaaacc aaaatgaaag tttaatcaag cagtatgtta agcgtcgtgc
    34861 aaagcgacgc ttatttttgt ggttgtttgg tacttctgca ggtttgatta ctattttgat
    34921 tactgtgttc gtgacccttt ttctaatact tgcggcgggt tcgattgata attctgattc
    34981 agattcaagt agtggtggcg aagcttttac aggagaatat tcggaaggct taccgattta
    35041 taaagaaatc aaaggaagag gccctttttc tgatgagatt gcgcaatatg ctgtaggtgc
    35101 agcagtgaaa tataaattat taccaagtgt gatcctttcg caatacggtt atgaatcagc
    35161 gtttggtacg tcggcgtccg caagaaatga tctcaactat tttgggatta cttggtttga
    35221 tggttgtctg tttcctaaag gaactgcgcg aggaatcggt ggtattgaag gtggctggta
    35281 catgaagttt cccaactcaa aagcagcctt ctcctattac ggctttatgg tagccacaca
    35341 atccaatttt aatgcatgtg taggaaataa aagccctggc gcaagcttat tgatcttagg
    35401 acgaggtggt tatgccgctg caggaattac tgaagatagt ccgtattata caggttgtat
    35461 gtccattatc acttctaata aattaaccga atatgatgaa ttcgccatca aacattgggg
    35521 tgaaggtggc aataacaacg gcacgatcac tggtgaatgg acgaatccat tccctggcag
    35581 cagtttagat aaaagttcct tcagtggtgg tcaattattt ggaacaaatc caggcggtga
    35641 attccgtccg aatggcttcc atgatggatt agactttggt tcagtcgatc atccaggaag
    35701 tgaaattcac gcggtgcatg gaggaaaagt cgtgtacgtt ggcaatcctg gtatttcagg
    35761 tttaggtgca tgtgtgatcg tgattaatta cgacggtttg aatatggtat atcaagaatt
    35821 tgcgaatagt acaggaaatt ctcgtgtgaa ggttggcgat caagtgaaag ttggtcaagt
    35881 aatcgggata cgagatacag cacacttgca tttaggtttc actcgaatgg attggcgtca
    35941 agcacaagga cacgcattca cagacgatgg tacatggatt gatccattac catctttgaa
    36001 tagcagcaaa aaatagatca ggagaaaaaa tatggaaaat aaaactttaa aaatcgtctt
    36061 aacagctgca attagcgtta gtttaatcgg caatatcgcg ttgttttggc agctgaaaga
    36121 agaacaagag acaaatacct ttaaaattga agaagtggct aaaaacgcgt ctgagaagct
    36181 caaaaaaaat tcctctgagg cacaggcgaa gatcgacaag ttaaatcaaa ccatcaatga
    36241 gaaaaaccaa gaaatcgatc agctgaagca acaagatggc acaaaagcaa aagaggaagt
    36301 aactgaagca caaagaaaga cggcagaaga gttcggtcaa ttggccattg ataaatcatt
    36361 aagtagaaat gaattaacgg aaaaactaaa agatgtcgca acccaagaag tgatcgacaa
    36421 attaagtcct gcagacgatg atcaccaaca tgatgattac ggaagttact cctttacatt
    36481 aggtgacgtg caaacttatg ctcaaacaag cagtgtaaaa gaggaacaaa attttgtcgt
    36541 atttgttgat tatacaatcg gcaatccgca attcaaagat gtcaaaccac aaaaaataaa
    36601 aggtggatta actgtcactg aaaaacaagt agatggtcaa tggaaagtca tagactttag
    36661 ttatttcact cgttaggagg aagaaaatga gtaagaaaaa aggagaaaga caacgtttca
    36721 aaaaacgaaa aagttatctt caatggctgg gaatctttat gattctcggc tttttgattc
    36781 ttgctagtcc gatttatacg aatcagaagg tagatatacc agatgaggta agcacgaacg
    36841 cctttttaaa cacgaaagag ctgacaatgg tttcaaaaga aaaaggagcg aaccagcaat
    36901 tagtttttga atttgcgatc gatgatgaag atcaaagctt gttaaaagaa ctggctaatc
    36961 ttaattatcg cgtagaagta aagacagcta aaggggatta tcaagcaata cgagccaaag
    37021 tgatcaaggt gtctgatgat tattttgcag tgaagctaac gcatgtacca gaagaatata
    37081 tcgccatgcg tttaaccatt attcctgaaa aaattgatcc aaaagtggat atgcaagaac
    37141 cacaagatct gatttattac gtgcacgaag ataaggttaa agatcaagtg aaagatgaag
    37201 attatgaaca acacgcgatc aattacaaag tgaaaggata caagaaagaa caaaaagcag
    37261 ctcaaaaaca aatggaatct ttccaagcaa ccattgattt gaatgaagaa ttagtgaaga
    37321 agctaaaaga tcaattgcct tatcaagtcg cagatgatca ggaaaatact gaaaacaaga
    37381 ttaatagcta ccagcaagaa attgaaacat cgaaaacgca actgaaagat caagaagaga
    37441 tcattcaaaa acttcaagaa aaaattgatc gtatgacaaa agaaaagatt tagaaggaca
    37501 ggtggagtga aaaaatgaag cgaacaaact tggctgacaa agatactttc gctgagcaga
    37561 aagtattgat taaagcctat ctcaaagttg taaaagctaa agaaatatgt cgagaagtga
    37621 atttatctaa cacaacaata tctgagttga agaatggtcg aaagaattta gatactttaa
    37681 aattcagatc atttgaagct ctatatgtag ctgctaaaaa atattttaca gctgataaca
    37741 aataaaaaac aggagtattt tatgtgaaaa aagtatataa aaaatgctta tgaatcagct
    37801 atatcacctt aaccatttgt taatgagatg ggagaaaaca ttaaccacga ttctaacgca
    37861 aacatcaagc acataaaaca tttaacactc ctcctttctg gctgaagatt tcaaccaaac
    37921 aggaagacag cgtatcaatt atagcataca ctttttctta aattcatttt tcatatgaca
    37981 aaatatttcc ttctaatcga caaagttaga aggatttttt attgtctaaa tacacaaata
    38041 accttaattt ttaaagagca taaggaaacg tgatatcact ttctcacgaa aggtgttagg
    38101 taggctgtgc ctaccacgtt tctaagaaag ataaatgata tcaaaatttt ggatttgtgt
    38161 gtagatttgt gggtaggatg aaagcgaatg tgggcagtaa aataagcgtt tgtgtgtagc
    38221 aaaatcaaaa aatgtgtagg gatatgtgta gctacacata tccctacaca taataaattt
    38281 ggaggaaaat atggacttat taattagaaa tattgaagaa aagtatatta ataaaattga
    38341 caaacgttgt gaagaactta cggtcaagac tggcaaaaaa tggagtcgaa atcaatacct
    38401 aaaagttttg atcgaaaatg attttgatca cgctcttctg aattataaaa aagaccaatt
    38461 tgatcggttg ttagaaaaat ttgtggatat tcaaacttat aatacgaaaa ctttagaaga
    38521 atatattgca acaaataatc aattaattgg tttgttaatt gaataataaa aatagtattg
    38581 tgaatttata aaaggaggaa atcaattttg gcaaaagcaa caagaaaaca tgtatatctt
    38641 tacgatatgc atatcgaagt acttgatgaa ataatttcta ctcatgaaga tatatctaat
    38701 tatagtgaag cagtcagaca tttgtgtatg atgtatgatc caaaaatcga agaaaaaata
    38761 aatccaattc aaattaaatt gaatgcaatg ggaaaagaaa tttctatttt aaccgaaatg
    38821 gtttcagagt ttgcggatct tcatttgaaa gatacaggaa tactaattgg tacaaattca
    38881 actgtttatc aagaagcaga agaacgtgtg gaaggaaaaa ttaaatccaa tcaaaccaaa
    38941 aaatattctt ctaagttaaa gaaaccagtg aatgaatctg ttcataccag agtagatcca
    39001 tttgatccaa cgaagattct aaaaagataa aagtagaagg agaacgtgat gaataaatcg
    39061 cctgcagtta ttcttacttg tcaatttacc ttgcccaata aaaaatcttt ttcaacatac
    39121 attgattata tgacaagaga aaaagcactg gaagaaatgg aacgacgtac gcccgaagaa
    39181 gaacaggaac tgcaacgaat caagaatgct ttagaggatt tttatattcc tgaaggagaa
    39241 actttttcgg aaagtaagac ggataaagaa ttcagtgaaa cgtcgaatga agctcaatcg
    39301 ttgatggaac atggaatcaa ttttgacaag ttagaggaac aagattttac caaatatctt
    39361 tcttatatga cccgtcacta tgcgttagaa caaaagaaag aactgacgca aagggaacaa
    39421 aaagaatacc aacggttgaa ccaagcaatg aaaaaatatc aaacagagcc acataaaaaa
    39481 gcttcaggaa atcttcctgg agttttttct atggcttcag acgaagtgaa aggaagtgat
    39541 ctgaaggaga tcaaacaaat cttccaaaga ggacaacaaa aaggctcaat catttatcaa
    39601 gatgttatct ctcacgacaa ttcgtatctt gaaaaaatgg gcttgtacga tcccaaaaca
    39661 gataccttag atgaagaagg tttgaaagca gctggaagaa aaatgatgga gacattattc
    39721 gagaaagaac aattgaatga aacaggttac tggatggcaa cgattcaccg taacactaag
    39781 catatccata ttcattttgc agttgttgaa aaagaaaata cgcgtaaacc gtatactgag
    39841 attgaaaatg gtgtgagtta tatcgtaccg aaggggaaga gaagacaagc aacattagac
    39901 gcaatgaaaa ccaattatgt acatgagtta gaacgtttct cttatgaaaa ggatcgaatg
    39961 atcggtatgg agaaacgaaa tttattgaca cgaaaaagtg atttgcgtaa caccttaaca
    40021 aaagtagtaa aagaaccagc acgttacgat cgacacgcca tgaacttatt aaatgaagta
    40081 tataaaaccc tacctgaaaa acgttcggaa tggaattatg gagatgaaaa tcgaaccaag
    40141 ctttcaccat ttacaagggg aaaacttgat gagttaacca aatatgtttt aacaaaagaa
    40201 cctggatatc aagaatatat acagatttca aaagcattga aagaagaagc caaaagcctt
    40261 tatggggaaa gcaaaagaga aacaaagaat gcagaaaaaa acgccctctt tgatttaaaa
    40321 aagcgcacag gaaatgcgat tttaacagaa ctgaagagac aggatagtca aataaaacca
    40381 ttaatagaaa gtgtcaatca gatgcaatct atttacaatg aacgatcctt tcgaacactc
    40441 aaacagttgc caacaaatga atacaagtat agttatcaag aattcgtaaa gcgtaggaaa
    40501 caattccaac aaagagttgt cacgcagcga agcttgcgtc aattaaatcg aacaattaat
    40561 gcacatgaag aaaaatacca cgcaagtaaa gcatatgaag agcaacagcg aagaatccaa
    40621 atggaacaag aaagacaata ggaggaagag aaatgaaaca atatttagat caatggaaag
    40681 tcatagaagg atcactaaaa gaagaacgaa tcgaacaact tcctgattgt ttagaaaaag
    40741 agcatttgtt tcagataagc gaaatgttga gaaacgaaca gtttgatcca aatcaatttt
    40801 tagttgttga gtacccagct actggtgtgt attgttgtaa tcatgtgaag ggtgaaaaat
    40861 attttattat tcaagaatac gaaggtaagt tagcgcctta ttatactact tgggaaataa
    40921 atgaagaggg aatcaataat ttcccatgtg aaagcattga agagtctatt tctctcacgg
    40981 aatgttaaat aaatagagac gtaaaagagt cctgaacgtg attgtccagg actctttttc
    41041 aatgaattgt tttccgccaa ttcactgagt ggtgcgattc aattttatta tatcaaaaga
    41101 aagtgagatt ccaaagtgaa gggaggaaga agaaatgact cagacaacga ttggtgttgt
    41161 ggttgaaaaa agcagggata atctcgtttt tgtgacagaa attcaaacag gtagagcttt
    41221 tgtagtcaca gacaaagctg caaaagcgta tcaaaatggc aatattttaa cgttaaatct
    41281 gactacgaag atgttcgttg atgcagtaga aaattatcca tttgtttaga caaacgatat
    41341 tttacaagga ggaaacggac atggcagaag aaaaagaacc actagataat agtcgtttgg
    41401 gaaagtccaa aagaaaatta gtacggttgc aaaacgaatt aaataatcaa tttcaaaagc
    41461 tatttgatca ccaaaaattg acaaatgggc aaccaatgaa tgacaaacga aatggtcgtt
    41521 catggtttaa ccagcaagaa aaaatagaaa ataaaataca aagtttgaga gaagagatca
    41581 agcaacaaga aaaacaggta gaaaaacttg aaagacaaga agagctaaag gaaatgggtt
    41641 ataacaagta cggtggcctg gacatgacga tcgaaaatat tccaagaatc aaagaagaaa
    41701 tcgaacgctt tgaaaaaggt gaatcgactt tttcagctgc cacgatccga aaataccaac
    41761 gaaaattgga aacattagaa cagctgaaag aaaaatcaga gaaaggaaaa gaaaatcttc
    41821 ttcctgaagt acaagcgatc attgacagcg gacgagtgac gcaatggaaa aagaatccaa
    41881 ctatctattt tctgaaaggc tatcgaaaag tcgctttaga actcaatgaa aacgggggat
    41941 ttgaggaatc taaaaaatac caagccacgt cggaagaaga aaaggcagct gttcgtgaat
    42001 tacttgaaga agctacacct actgattctg tgaaggagaa agaaaccgat catttctatc
    42061 gagtagaatt caacgaaaat gatccagcaa aagggttgaa aagttatggt ggggagatcg
    42121 taacactaga attgatcgat caactcaaaa cactggataa ccaatacgcc gcagaagcag
    42181 gctattataa attctatttt gaagaagtga agaatggaga agtagtcgat cagatacgaa
    42241 tggatctagg ggatggcgtc aatagtaatc accccatcta tttagagtta gctagattgt
    42301 gcgaacaatc acctgaaaag gaacaagaac aaacagtcaa aaatgaacct atttcaaaag
    42361 tacaaaacga gattaaagag acaaaaacaa aacaaaaggg ttatcaaaaa cgaacggcag
    42421 aagagattaa gcaagaagtc aaatcacttt cccaacaggc gcttgatgcg gtgaaaaagc
    42481 atactcattc acctgaagac gtcaaagaac ttttgaattt tatgtctcgt tttccagaac
    42541 gatcctttag aaatcaatta ttgatcgagg aacaatttcc aggcgcaacg gcttgtttag
    42601 gtcgtgccgc gttgaaaaaa gaaggaatct atatcaaaaa aggagaacaa gggaacaaga
    42661 tctttgtcag aaaaacggtc aaaggatttt atgagaaagg acgtggcttt gttcgagaaa
    42721 cagaggctac accagaagat aaaaaacgaa tcgaaagtgg tcaaatcgaa gtgatcaaaa
    42781 aaccttacta cacgattgaa aaagtgtttg atatcacaca aacacaattg aaacctgaag
    42841 actatccgaa aatcttcccg aaccgcgtct ttgattttca attggatcaa acagggcaga
    42901 cagaacttca agttgggatt caagcggtag ccaaaaatat tggaattcaa attcgagata
    42961 tgacagaaag tgaagtctat caaagagaat taggacaagc acgtggggcg tacgttcgta
    43021 acgaatttac tggcgaagaa gaaatcgtca tgaatactcg aaatactcct acgcagcatt
    43081 tagccacaag tattcacgaa ttagcccatg ctagaatgca caaattttca gaattagata
    43141 ctgcaacaaa agaactacag gcagaaatga cctcctatat tgtgtgcaag cattttggga
    43201 tggacacctc ggagaaagct attccttata ttgcgacatg gacgaagaat agtcagacgt
    43261 tggatgataa ggaagctgcg gaacgtggga aaatcatgaa tgatgtcagt cgtgtcgcaa
    43321 atgaattcat tcaaaccatt tctaatgaaa tcaatcaata cagagaaaga gaaccagaga
    43381 tccagccagc agaagtgaag caggagcata aaaatactgt agaaacggca gaacaaaaga
    43441 atattgtgcc cgttggctca ctttggatcg acaagaaaga cgggaaagtc ctggaagaag
    43501 acacgggccg aacgttacat ttgaaggatg cggcgtttat tgaagatccg cagcataatc
    43561 aagtgatttt aaaaggaaaa agttatggaa aagagatcca aacggcgtta ccttcagaag
    43621 aatttgcgct aggaaatcca ttccgatttt cacttgagac agtgtcagaa gtggcaaaac
    43681 gaaaaaatga aatctattat gaacaacaat tgtcacaaaa agaacgcgta taaaacaagc
    43741 aagaaagact atctgaaaaa tggatagtct tttttctttt gggaggtttg aaaatgacag
    43801 agaaaaaaat gagtctcatt gatcgatgca agcaaatgga tattgtggat tttgcacgaa
    43861 acaatggttt agcggtgg
//
LOCUS       DAJSAW010000021        43606 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJSAW010000021 DAJSAW010000000
VERSION     DAJSAW010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.021"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(245..2626)
                     /locus_tag="ONT64_001226"
     CDS             complement(245..2626)
                     /locus_tag="ONT64_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCR2302656.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2616..3263)
                     /gene="recU"
                     /locus_tag="ONT64_001227"
     CDS             complement(2616..3263)
                     /gene="recU"
                     /locus_tag="ONT64_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCR2302657.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3327..3866
                     /locus_tag="ONT64_001228"
     CDS             3327..3866
                     /locus_tag="ONT64_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCR2302658.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3956..4375
                     /gene="gpsB"
                     /locus_tag="ONT64_001229"
     CDS             3956..4375
                     /gene="gpsB"
                     /locus_tag="ONT64_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCR2302659.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4397..4764
                     /gene="rnpB"
                     /locus_tag="ONT64_001230"
     ncRNA           4397..4764
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ONT64_001230"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4973..6139
                     /locus_tag="ONT64_001231"
     CDS             4973..6139
                     /locus_tag="ONT64_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCR2302660.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6185..7681
                     /locus_tag="ONT64_001232"
     CDS             6185..7681
                     /locus_tag="ONT64_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HCR2302661.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7762..8445
                     /locus_tag="ONT64_001233"
     CDS             7762..8445
                     /locus_tag="ONT64_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR2302662.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9090..10280
                     /locus_tag="ONT64_001234"
     CDS             9090..10280
                     /locus_tag="ONT64_001234"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR2302663.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10382..11335
                     /locus_tag="ONT64_001235"
     CDS             10382..11335
                     /locus_tag="ONT64_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR2302664.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11404..12753
                     /locus_tag="ONT64_001236"
     CDS             11404..12753
                     /locus_tag="ONT64_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2302665.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12936..13259)
                     /locus_tag="ONT64_001237"
     CDS             complement(12936..13259)
                     /locus_tag="ONT64_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302666.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13388..14551
                     /locus_tag="ONT64_001238"
     CDS             13388..14551
                     /locus_tag="ONT64_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR2302667.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14621..15970
                     /locus_tag="ONT64_001239"
     CDS             14621..15970
                     /locus_tag="ONT64_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR2302668.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16015..16446)
                     /locus_tag="ONT64_001240"
     CDS             complement(16015..16446)
                     /locus_tag="ONT64_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2302669.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16834..19476
                     /gene="alaS"
                     /locus_tag="ONT64_001241"
     CDS             16834..19476
                     /gene="alaS"
                     /locus_tag="ONT64_001241"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR2302670.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19676..20272
                     /locus_tag="ONT64_001242"
     CDS             19676..20272
                     /locus_tag="ONT64_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR2302671.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20394..21542
                     /locus_tag="ONT64_001243"
     CDS             20394..21542
                     /locus_tag="ONT64_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR2302672.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21580..21993)
                     /locus_tag="ONT64_001244"
     CDS             complement(21580..21993)
                     /locus_tag="ONT64_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCR2302673.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22072..22503
                     /locus_tag="ONT64_001245"
     CDS             22072..22503
                     /locus_tag="ONT64_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HCR2302674.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22602..22835)
                     /locus_tag="ONT64_001246"
     CDS             complement(22602..22835)
                     /locus_tag="ONT64_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HCR2302675.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23018..24688
                     /locus_tag="ONT64_001247"
     CDS             23018..24688
                     /locus_tag="ONT64_001247"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCR2302676.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24835..25221
                     /locus_tag="ONT64_001248"
     CDS             24835..25221
                     /locus_tag="ONT64_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302677.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25208..25906
                     /locus_tag="ONT64_001249"
     CDS             25208..25906
                     /locus_tag="ONT64_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR2302678.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25911..26375
                     /locus_tag="ONT64_001250"
     CDS             25911..26375
                     /locus_tag="ONT64_001250"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR2302679.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26372..27283
                     /locus_tag="ONT64_001251"
     CDS             26372..27283
                     /locus_tag="ONT64_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR2302680.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27518..28057
                     /gene="pyrR"
                     /locus_tag="ONT64_001252"
     CDS             27518..28057
                     /gene="pyrR"
                     /locus_tag="ONT64_001252"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCR2302681.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28071..29354
                     /locus_tag="ONT64_001253"
     CDS             28071..29354
                     /locus_tag="ONT64_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR2302682.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29367..30293
                     /locus_tag="ONT64_001254"
     CDS             29367..30293
                     /locus_tag="ONT64_001254"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCR2302683.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30312..31592
                     /locus_tag="ONT64_001255"
     CDS             30312..31592
                     /locus_tag="ONT64_001255"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR2302684.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31597..32673
                     /gene="carA"
                     /locus_tag="ONT64_001256"
     CDS             31597..32673
                     /gene="carA"
                     /locus_tag="ONT64_001256"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR2302685.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32678..35863
                     /gene="carB"
                     /locus_tag="ONT64_001257"
     CDS             32678..35863
                     /gene="carB"
                     /locus_tag="ONT64_001257"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR2302686.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35917..36693
                     /locus_tag="ONT64_001258"
     CDS             35917..36693
                     /locus_tag="ONT64_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HCR2302687.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36690..37622
                     /locus_tag="ONT64_001259"
     CDS             36690..37622
                     /locus_tag="ONT64_001259"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HCR2302688.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37619..38335
                     /gene="pyrF"
                     /locus_tag="ONT64_001260"
     CDS             37619..38335
                     /gene="pyrF"
                     /locus_tag="ONT64_001260"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR2302689.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38342..39031
                     /locus_tag="ONT64_001261"
     CDS             38342..39031
                     /locus_tag="ONT64_001261"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR2302690.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            39004..39273
                     /locus_tag="ONT64_001262"
     CDS             39004..39273
                     /locus_tag="ONT64_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302691.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39311..39952
                     /locus_tag="ONT64_001263"
     CDS             39311..39952
                     /locus_tag="ONT64_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR2302692.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            39995..40687
                     /locus_tag="ONT64_001264"
     CDS             39995..40687
                     /locus_tag="ONT64_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HCR2302693.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40684..41586
                     /locus_tag="ONT64_001265"
     CDS             40684..41586
                     /locus_tag="ONT64_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2302694.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41744..43450)
                     /gene="efbA"
                     /locus_tag="ONT64_001266"
     CDS             complement(41744..43450)
                     /gene="efbA"
                     /locus_tag="ONT64_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HCR2302695.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13967 a         7688 c         9479 g        12472 t
ORIGIN      
        1 aaataaaaga ctagtgtgta tttagccgat ttaattataa acaagtaaat ggataaagta
       61 tatgtaatga taaaagaaaa atggattttc taatttttaa gctaaatgtt agatcacaca
      121 tgtatatgct ggatagtctt ataaaaaatc atgtaataca gcttggtaca tgatttttat
      181 ttgtagctgt tttttacata gacacaaaaa agaaaccacg cttgtagacg tggtttcttt
      241 tttactatct gtttccagaa gctgaggtgc tgctggatgg tgtatggtca tttgcagcat
      301 ccgctccgct agttgctggc tcactggaag aagcaggagg agcagatgaa gacggtgtcg
      361 agctttcttc ggatgaagaa gaaggaaccg ttgttgaaga cgctggctcg cttgtttctg
      421 ctgtcgtgct ttcttttgag gactcagcag ttgattcgct ttgactagta gagctagagc
      481 tttgagtcgt tgtctctgtt gttgagcttc caggagtctg gacatagctg gaagatggaa
      541 tagttgttga tggtgtaatt gcattcgatc tagctgtata ttgatctttg tagtacagtt
      601 caccgcccac acgaattaat ccgcttggca tctcccagtc tgtatttgtc acgttagctg
      661 aaacatactg cataagctcg cgataaacat cagaggctac gtgtgacgat tctgaagtga
      721 caggtgtcat ttttttattg taacctgtcc acacggaaat cgaataattt ggcgtatagc
      781 ccgcaaatag gatatctgga tacaccccag aagaaatacc aagttttgca tactcgtcat
      841 ctgtgtaatt ggatgttccg gtcttaccgg cttgatataa cccagcgatt tgcgcattcg
      901 taccagttcc ttcagtgatc gtatctttta aaatgtctgt gatcatataa gctgtttctg
      961 gagacatggc tgtttttcca tctggctcat aagtttcttc tgttccgtct tggaagacga
     1021 ttttattaac atattgaggt ttgtaataag tccctccatt ggcgaaagcc gcataggctg
     1081 ctgccatttt taaggaagaa gctccgtatt ttgtcccatc ttgttcttcc gtattactag
     1141 agatcgcatt cgattgatgg atcgtactgt attcgatccc aagatttttt aagaaagaag
     1201 ccactttatc tgaaccgact tcattgaata atttgactgc tggaacatta cgggataagt
     1261 aaagtgcttg gcgtaaagta atggtgccca tatattgatt atcccagttt cctacaggag
     1321 tcgaagtccc ttcatagtta tacggtgcgt cagtaatagt ttttccagta gaatatttca
     1381 aatattcaaa agctggtccg taatctgtga caggtttcat cgtagaacca aaatcacggg
     1441 aagtattcac tgctaagttg ttgcctaaag ttacgtcttc tgcgatgtga cgtccgccaa
     1501 tctgggcttt tactttcccg gtattggtgt cgataagggt ggaggcaact tgcatctcgt
     1561 cgtctgggta agagacatac tgatccgtgt tgacgatatc atatagtttc ttttgggcgt
     1621 ccagatccaa attcgtataa atctctaaac cgtctgtgta gacattctta tctgtttttt
     1681 cttgtacttc attgatgact tctttgacat aattatcgac gatcttagta ttgtcatcgg
     1741 attgagttag ttcttgcaga ccatccgtta ccggtacatt aacagcttga tcatattctg
     1801 tttgagagat tttttcgttt tgaagcatcg tatacaaaac agtatctcgt cttttcttcg
     1861 cctgatctgg ataaacataa ggatcatagg cggaaggggc ttgaggcatc ccagcaagca
     1921 atgctgtttg cggtaaagaa agttctgata atttcttacc aaagtacatc tcagaagctg
     1981 tctccatccc gtacaagccg tttgacatat acactttatt tacgtagtag gtcaagatct
     2041 cttgtttcga ttttttttgt tccaatctta cagccatcca cgcttcttgg gccttgcgtt
     2101 tcagagtctg atcctcggca ctggtggaga agaaggaaag tttgatcaat tgctgtgtca
     2161 aagtacttcc gccttgcagt ccgccagagg taaagttaga gagggcagaa ccgatgatcc
     2221 gaatagggtc aaccccgatg tgtttataaa aacggcgatc ttctaccgac acgatcgcat
     2281 cttctaatgt tttcggtaat tcattcgcag aaattttttc acgcttttct gcgcctaaat
     2341 cttcgaatag ttcgccatct tgtgtatata gtttggaaga tacggtagcg tctaattttt
     2401 tatccgtcaa ttcgggcgca tcttttgcgt aataccagaa aagtcctaca cctgctaaaa
     2461 agaggataca gaaaaatgag agtattccta aaaggatctt gataaataat ccttttttat
     2521 gtgttccaga ggactttcca gaacctttgc cagaattctt cttcacactc tttttaggag
     2581 taggctgttt ctgtcgtcta gagcttctag tttgttcatt tgccattctt gctttctcct
     2641 tcaatagata atgttgataa atagaagtca accgcgtcca aataaggaat ccgcggagaa
     2701 ataccagttt tgatctctat tcctagttct tcgatcaaag ataaaggtag tgatttgcgt
     2761 cctttttcct tttgggcgtc ccagtaagca atcaaatgaa cactattcaa ataaaaacag
     2821 cgcttcaaag acgaaaacca taacaagaca aagcagaccc cattttgttc gaggcatgct
     2881 ttcatatgtt cgatttgatg ggcatggaag tttttaaagg gaaaagatgt tttgttcttg
     2941 gtttcttttg cttcaaagtc caagtaaaag cctcgataga cgccattata gtctgtagtt
     3001 gaggcttggg taaagtaggc ttccttgatc acagcagcac ttctcttagg atagtctact
     3061 ttcacgattt ggataggggt aggcttttta tggataaccg ccaacccgcg ggaaagataa
     3121 tatttattac tttcattgat tgcttcttca aagcgcatcc cccgattgcc gaattcagtg
     3181 atctgatttc ttttttcttg aattttttcg acgggagagg cattcttata ataaggttgg
     3241 ccatttggat agcgcaatac catctttaat cacactccta ggccacatta tagcaaaaaa
     3301 gcggatagaa agaaaaggaa tctgagatgg ataagtcaaa agtaatctat gtgtctgggt
     3361 atcgaagttt cgaactcggt gtttttaaag aaaatgatcc taaaatccaa gttgttaaaa
     3421 atgttttaaa aaaagaactc catcagttag cagaagaagg gctggaatgg gtacttgtca
     3481 gtgggaattt gggcgtcgaa ctatgggctg tgcaaatagt agcggaattg aaagaagaat
     3541 atccggaact atctcttggt attttgtatc ctttttctga ttttggtaat aactggaatg
     3601 aagggaacca agaaaaaaaa caattagcag aacaattagc agattatgtc gaagctgtta
     3661 gccatcaacc ctaccaatca cctagtcagc taaagaatca cactcgtttt atacttgagc
     3721 atacagtggg ctgtctgctg atctatgatg aagaattccc tggaaaaacg aagtattttt
     3781 ttgaagatgc acagaattac caagcagatc atccctacga aattcggctg atcagtatgg
     3841 acgatcttca aaattttagt gaataatcga tattgtgttt gatttatcaa cttttttcct
     3901 atacaatgaa aaaggtagaa atgttattac gtaaatgaaa actagaggtg taaatatggc
     3961 gaatttggtt tatagtccta aagacatttt acaacaagaa ttcaaaacaa aaatgcgcgg
     4021 ctatgatccc gttgaagttg atgaattctt agacaatatc atcaaagatt atgaagctta
     4081 cagcaaagaa ctccttgctt tacaagaaga aaacgatcga ttgagtgcaa aagtagctca
     4141 attatctaaa actcaaggag cagctcaaac acgcgtgcaa caaacagagg caccaaagag
     4201 cgcagcagtg accaattttg atatcttaaa acgcttgtca aatcttgaac gtgaagtatt
     4261 tggtaaaaag cttgatcaac aagcaagtgc agtcaaacca gcacaaccta atccaaataa
     4321 ctacacaaat gcagacacaa gcttagatga taatgaaaaa acacgtcaat tttaattttt
     4381 gattaatgaa tttgcggttt tcgggtaatc gcggtctgac ttgatcaggc tgaggaaagt
     4441 ccatgctcgc acaggctgtg atgcctgtag tgttcgtgct tagcgaaacc ataagctaag
     4501 gtactctttg gagtaacggc aggaaaacaa gctaaggctt cggctatgct taagtatcct
     4561 tgaaagtgcc acagtgacga agcggctggg gaaacccatc cggtggaacg cggtaaaccc
     4621 ctcgagcgag caacccaaac aatggtaggg gcgctttttg tctggaagaa gaacaggaca
     4681 aaaaggcagt ataactgcag atagatggtt accgctttgt tttttctccc tggaaaagca
     4741 aagtacagaa catggcttat agaaaaccgc aaacatcagg gaattaggtc gtgacaaaag
     4801 taatgcgtta taagaaacaa gaaaaacatt tgaaagctat ttttcaatac ttcagatgaa
     4861 tcgttcttat tacaaaacaa acttttgtat caccggttat tcggaatttg agtaaaagtg
     4921 tggggtagga ctgatgtccc acctcctttt tttataaata aggagattgt gtatggaaaa
     4981 aaacaggaag ttcaacctcg tcgcaactgc ggcaagcggg ctagaagcac tggttggtaa
     5041 agaattaaga gatcttggaa tcgaatgcca agttgagaac ggacgtgcac gttttgaagg
     5101 aacaatggaa acgatcgcta cagctaattt atggttacgt acagcagatc gagtaaaaat
     5161 cgtggttggg gaattcgatg cgtatacgtt tgatgaattg tttgaaaacg taaaagcatt
     5221 gccttgggaa gattttcttc cactagatgc agcttttcct gtagctggaa aatcgatcaa
     5281 gtcgaaacta tatagcacac ctgattgcca agcgatcaca aaaaaagcaa tcgttgaacg
     5341 tctccgtacc tattatcacc gtccagcttc tgtaccactg acggaaactg gcgcacattt
     5401 tcagttggaa gtagctttgc tgaaagacca tgtgatggta actctggata cgacaggacc
     5461 aagtttattc aaacgtggtt atcgcttaga aaaaggtgga gcaccattga aagaaaacat
     5521 ggcagcagca cttattatgt tgacaaattg gcgaaaagac cgtccatttt atgatcctgt
     5581 atgtggttca ggtacgcttt gtattgaagc agccttaata ggccataata tcgcaccagg
     5641 attcaatcgt gagtttgctt gcgaatcttg ggattggttc agtcaagaaa tcatggaaaa
     5701 tgttcgatca gcagccgaag aaaaagcaga ttatgatatt gaattagata ttactggttc
     5761 agatatcaat ggacggatga ttgaaattgc caaagccaat gcagaagaaa tcggattagg
     5821 ctcttcgatt acctttaagc aacaagcagt caaagacttc aaaacggata aagaatacgg
     5881 ggtcatcgtg gctaatccgc cttacggaga acgtctagga gaagaagaaa ctgttcggaa
     5941 attatataaa gaaatgggcg atgtattccg tccattaaaa acatggagta aatatatctt
     6001 gactagcgac ttagcgtttg aggaatttta cggacaaaaa gcaaccaaaa aaagaaaatt
     6061 gtacaatggt gcattgcgta cggatctatt ccagtattgg ggaactcgtc cgcctagaaa
     6121 aaaagagaaa gacgaataat cttactaatt gattcattca attggatgtt tggaggaata
     6181 gggaatgaaa gaaaaagagt ttttacaaga agtaaaagaa atcaatcttc tacaacaagc
     6241 tttaggaatc ctcgattggg atacacaaac aggtatgcct gaaaaagcta gtgcataccg
     6301 aagtgaagta gacagctatc tttacagcat ttatttttca aaaaaaatcg gtccaaaaat
     6361 caaagaagcc attcgctatt tttctgaaca tccggaagaa ttgtcggaag ttggaactgc
     6421 cgtttttgcc aaagtaaaag aagagtatga tttggaacat cgcgtaccgg aagatcttat
     6481 gagaaaatat acagctgccg tttcttcagc acacggacaa tggcaaaaag ctagagaaac
     6541 acaacaattt tctgattttc aacacgcatt gaatcaaaat attgaattaa caaaacagtt
     6601 gataccttac tggagaaaag atgaaaagac gaattatgat gttttgctga atcaatacga
     6661 acccgggatg actgtcgaaa tccttgataa ggtatttgat cagcttaaag aaggaattct
     6721 ttcgatccgc cgtacgctcc aagaaaaagg gactgaacct gaaacagact ttttaaatcg
     6781 gagaatgacg aaaaaacaac agaagagatt tgtagtgaaa gtaatcgaac aacttggtta
     6841 tgattttagt cgtggtcgtt tagatgacac tgtccaccca tttatgactg gaattaatcc
     6901 gaatgatgca cgaatcacga cacgatggaa cgaaacagat ttcaaaatgg ctgtttttgg
     6961 agtgatccac gaggctggtc atggtatgta tgaacaagac atcgatgata aatatgcgta
     7021 tacaccggtt tatgaaggta catcaatggg tatccatgaa tcacaatcgt tgtttaacga
     7081 gatcattatt ggaagtagtc gcagcttctg gaaaaagcaa tatcctttct ttcaagaatg
     7141 tgcagaagga acattcgatg atattccttt tgacaaattt tatcgttcat tgaaccatac
     7201 aaaatcaagt ttggttcgta tcgaggcaga cagtttgact taccctctcc atatcatcat
     7261 tcgttatgaa atcgaaaaaa tgattttcaa tgaaggggta gatgttgctg aactacctga
     7321 aatatggaat caaaaatatg aagagtatct gggaatacga ccaacaaatg atgtggaagg
     7381 catcctacaa gacgttcatt ggtcaggtgc aagctttggt tattttccct cttatgcatt
     7441 agggtacatg tatgcggcac aattacttca ttctttgcag caagaaatag atgtggaaaa
     7501 tgttctagcg tcagacgatt attctccaat caaaaactgg atgtcagagc atatccatca
     7561 atatggtgca tctagaaaac caaatcaatt aatcatggat gcaacacatg aaccgcttaa
     7621 tccgcaatac ctgatcgact atatgaaaga cctgtatttt aatgtatatc aggtaaaata
     7681 aaatacaatt tacaaagata gctgaaattc atctaatcta tagatgtttt cagctattta
     7741 cttaagaaag aagtggaacg aatggataaa ttacaagtga tcgctgatta tagtatggac
     7801 caattagcgc atgatcaaac aggacatggg agcgatcata ccaaaagagt tgtcaagtta
     7861 gctgaaaaaa ttttggagac ggaaccgcag gcagaccgct ttgtgacgct tgcagcagct
     7921 tacctacatg atacgattga tgataaagtc gttaaaaatg agaacgaggc aaagcaacag
     7981 cttcgtgtat tccttcgctc tttgccaata gtagaggaac agatctcgat gattttttcc
     8041 attatcgaaa atatgtcctt ttctaaaaat ctctctgaag cggccgaact atctctggaa
     8101 ggtaagatcg ttcaagacgc tgatcgtatc gaagcattag gagcaatcgg cattttgcgt
     8161 actgcttatt tcggcggtgg acatgggcac ccgatttttg atcctgaact gtatccgcaa
     8221 acattcaagg acaaagaaaa ttaccgaaaa ggcacgacag tgatcaatca tttttatgaa
     8281 aaactgttct tgctgtctga taaaatgaat actgattacg gatatgaaga agccaaacga
     8341 agagaaacct tcatgcgtga attcttagaa gaattttttc tggaatggtc tattgacgat
     8401 gaaaatttta ctccagacaa ttggatcaaa aataacaaaa aatgatggag gttgtgacag
     8461 aagtggtcag ctccaagata tttcggatca ttttccgtaa aagttacttc tggaacactg
     8521 tttaccaaga ggtcgtgagc ctgccgttca gctccgagtc acaaggaaca atttcaaaaa
     8581 atagcttctc gtgtttgatg aatattgtaa cttgtgattc ggaattggca ggagaacacc
     8641 gtttattcgg aagaatagga ctgtgacaag acttttgtca cagtcctttt ttcgaattca
     8701 acaagtaaaa cttttaagaa atattcagag agtaaaatta aaaattgctg ataaacgaag
     8761 atggaaattt tatgtatttt tattggtcta tgcaataaat aaattaaata ttcagaatac
     8821 aattgatttt aacgaaaggg cttctatcaa tggttgtgtt gtatttatct gcccaggtag
     8881 agtaaaatag atcattatct aaaaggtaat tgttcatact atataagtca tttgaaagta
     8941 aaaagatact gtaaataaaa aatgtggtta aataattctc ataatcagtg taagcgttat
     9001 caaaaatata gcatacttgc tttgtagatg aatgacacga tatatgtttg gtatgttctg
     9061 caagacagat gaatatggag gtggagacaa tgtcttccaa agaactaaca atgtatttag
     9121 agaaagaatt ggatcaactg aaagaaaagg ggttatacaa tacgatcgac atactagaaa
     9181 gtgaaaatgg tgcttgtatc atcgtagacg gaaaaaagat gatcaatcta gcatccaaca
     9241 attatcttgg atttgccaat cgcgaagaat tgaaaaaagc atgtattgaa gcaaccgaaa
     9301 cttatggtgt aggagcagga gcagtccgaa cgatcaatgg ttcgctgaaa atccatcagc
     9361 aattagaaga aaaaatcgca gaattcaaag ggacagaagc agcaatcgcc tttcaatctg
     9421 gattcaactg caatatgggc gctatctctg cagtaatgac aaaagaagat gctatcttat
     9481 cggatgaatt aaaccatgca tctattattg acggttgccg cttatccgga gcaaaaatca
     9541 tccgaattaa acatcaagat atgaaagacc ttgagaaaaa agcaaaggaa gcgatagaaa
     9601 gtaagaaata taaaaaaatc atgtatatca cagatggtgt tttctccatg gatggagaca
     9661 ttgcacgatt gccggaaatt attccgattg ttgaaaaata tggactgatc acctacgtcg
     9721 atgatgcgca cggctcaggc gtaactggaa aaggtgcagg aaccgtcaag cattttggat
     9781 taagcgacaa aatcgatatg caaatgggca cgttatcgaa agctataggt gttgttgggg
     9841 gttatgtagc tggatcgaaa acactaatcg actggttgaa agctagaagt cggccattcc
     9901 tattttctac ttcgctaact ccaggtgcag ctgcagctgc attggcttcg attactttga
     9961 tgcaagaaca tccggaactt gtcgaaaaag tttgggaaaa tgctaattat tttaaagaag
    10021 aattgaaaaa agtaggctat aacatcggta tgtctgaaac acctattact ccagtcattt
    10081 tgggagacga aaaagtgact cagactttct ctaagaagct gatcgaacat gggatttatg
    10141 ctaaaccaat cgtttatcca actgtgcctc ttgggacagg caggatcaga aatatgccga
    10201 ctgcggaaca tacgagggaa atgcttgatg aagcagtagc agtttatcaa aaaataggaa
    10261 aagaaatgga aattatttaa ctaaatttag ttttttcgtc tatttaaaat tagagaacat
    10321 gttcacgcat atattgttaa tcatctgaag atagtagtaa aaaagatcag ggaggatcat
    10381 catgaaaaaa gtattggtga caggatgttt aggacaaatt ggttcagagt tgatcacacg
    10441 gctacgtcaa gaactaggca gtgaaaatgt cattgcgaca gatatacgca tgcctgaagt
    10501 cattgagaaa aacgatatct ttgagatcct agatgtaatg gattacgaaa aaatgcgcag
    10561 catagcagaa caatatcaag tggacacatt gattcatttg gctgctcttt tgtcagctgt
    10621 tgcagaagca aaaccacaat tagcctggaa tttgaacgtg actgggctgg tcaatgcatt
    10681 ggaagtcgct agagaattcc agctgaaatt ttttacgcca agttcaatcg gcgcgtttgg
    10741 tccaaatact ccaaaagatc ataccccaca agatactctt cagcgtccta caaccatgta
    10801 tggggtaacg aaggtagctg gagaactact atgtgattat tattatgtga aatatggcgt
    10861 agatactcgt ggtgtccgtt tccctggatt gatttcttat gcaaccttgc caggaggcgg
    10921 tacgacagac tacgcagtcg atatttatta ttcagcaatc aaagaaggaa agtacactag
    10981 tttcattgat caaggtacat caatggatat gatgtatatg ccagatgcga tcgatgccat
    11041 tataaaactc atgaatgcag atcctgatcg attggttcat cgaaatgcct tcaatatatc
    11101 ggcgatgtct tttgacccgg aagagatcaa agcatctatt caaaaaatca taccagattt
    11161 tgaaatggat tatgatgtgg atcctgtacg tcagtcaata gcagaatctt ggccaaattc
    11221 actagatgtt tcttgtgcgc aagaggaatg gggcttttcg ccgaaatacg acttagatgt
    11281 gatgacaaag gatatgttgg aaaaactgaa agaaaaattg gtagagacgg tataaaacaa
    11341 gaaccacatc caaaaagttt tttatacttt tgggattggc tatggtatga aaggggacaa
    11401 gggatggaag aatatcaagc cacgccagta aaagaagtaa aaatagaaaa tgaattaaaa
    11461 cgtacgatgg gattttttac tgctttatcg actgttatgg gcacagtgat cggtgcaggg
    11521 gtatttttta aagccgcaag cgtagcagaa gttacaggat ccgccagttt gcatatgttt
    11581 tcttggtttc tcggtggtat gatttctgtc tgtgcaggac taacaggagc tgaactggca
    11641 gctgctattc cagaaacagg agggatgatt aaatatatcg aacggattta cggaaataca
    11701 gcagcttttt tattgggctg ggcgcaagtc gtgatttatt ttccagcaaa tgtagcagcc
    11761 ttgtcgatca ttttcggtac acagttcgtc aatctatttg gtctttccca gtcaatgatt
    11821 gttcccgtag ctgttacagc tgctgtatcg atcctgttga tcaatttttt aggttctaaa
    11881 gctggagggg cttttcagtc gattacgctt gtctgcaagc ttattccttt gtttgtgatc
    11941 gtcattttcg gtttgttccg acaagaagga gtagacttcc aactgtttcc gatccaagca
    12001 ggagaaaatc tttcattttt ctcagccttg ggagcgggct tgctagcaac gatgttcgca
    12061 tacgacggtt ggatacacgt aggaaatatt tcgggagaat tgaaaaagcc ggcgaaagat
    12121 ttgccaaaag caatttcttt aggaatcatc ggaatcatga tcgtttattt attagtaaat
    12181 gcggtgttct taagaaccgc atctatcgac ggagtcgcag gaaacagcaa tgcggcaagt
    12241 gacgttgcaa aaatgatttt tggcggcttc ggtggaagac tggtcacagt tggtatcttg
    12301 atttctgttt atggaaccat caatggctat acccttactg gaatgcgtct cccttatgtt
    12361 atggcaaaag aaaacaacct gccttttagc aagctctttg ctaaacttca tgataaaact
    12421 aaagttcctg tagccgcagg tatcttagag ctagtcatcg ctattggcat gatgatgatc
    12481 ggtggttttg atacactaac ggatatgctg atctttgtca tttggatttt ttatacgatg
    12541 gtctttgttg gtgtcattct tcttcgtaaa aaagaacctg atttgtttcg accatacaaa
    12601 gttccaatgt atccgtttat tccactagta gcaatcattg gagggacttt tatcgtatca
    12661 agcacgctta tcactcagac ctttttagct tcgatgggga tagtattgac acttgctggt
    12721 atcccgattt atctctattt aaaacgtaga tagctaaaac attcgaattt tattagaact
    12781 gcacttctct gccaaacaaa atattttatt taaatgagga gtacgtggaa gaaatgtcaa
    12841 aagtactcgt taattttgat cacgtaaatg tcgttttgtc tacgatgaaa tagcaaagta
    12901 aaagacagaa atcgggctca accgatttct gtcttttact ttgatttgcg taactgaact
    12961 attcggcgta cgccgcgata aatatttaat aaaaccaagc aaccagaaat gaaaatggga
    13021 agccaaatca gatagacagg gtagccaagc actttagaca aagcatgtgg agtcatttta
    13081 tccatttcaa gaagtgcttg ctgccaagtc tgaagattga agactagcat gaaacccgaa
    13141 ataatcagga gaataaatcc taaataatga attgtagttc tttttcttac ataagcgatc
    13201 gtagcataaa ggaacagtaa aaaaataggt acgccaaaaa caaagtacat attcagcatc
    13261 tcgacacccc ctttgtgttt atcttacttt actttgaaag aaatgagaaa taatagcctc
    13321 gttacttgct tgaaacagca aaggactgct aagataagag agatacaaag aatcaaggag
    13381 gagtcatatg gcaagttata tcgaaccgat tcgttttgcg ctgattgttt ttccattttt
    13441 agccttagcg atctcatctg ttttttttat ttatgaatat cgaaagtatg gaacatttct
    13501 agtaacaagg gcagttattt tgtattcttt cgttttttat ttattgtgtg cgtatttttt
    13561 ggtgatctta ccacttccgg caaaggagac ggttgcccaa atgacagggc caaagatgga
    13621 gctgcattta tttgcttcat ggcaacattt cagagacaac acggtattgg tcttgtctga
    13681 acctagtacc tacctgccgg caatgaagca gagtgtattt ttggaaccag cttttaatgt
    13741 tttattactc ataccatttg gcatttattt aagatattat ttccggcttt cttttatgaa
    13801 aacgatgatt gctagtttct gcctttctct attttttgaa ttgacgcagc taagtggact
    13861 ttattttatc tacccaagac cataccgctt atttgatgtg aatgatttag tgacaaatac
    13921 attgggtgga atcattggtt ttgtcgttac gccgttattt acatttatgc ttcctacaag
    13981 aagacgaatg gatgaacttt cttatgaaaa aggaaagact gtctcattga ctcgacgctt
    14041 ggtcgcttat ttgatcgact ggacattttt atctcttatt aatttggtag cggcgatttt
    14101 ctttaaaatt gcgttcgatt caggggcagt tacttctcat atctggtggc ttttattaga
    14161 agtaggggta tactttatga ttgtaccgtt tataaccaat ggatggacac ttggcaaaaa
    14221 aattgtgcgt atccgtttgg tggaagaaga ccgcgaacga atttctttaa aagcattgat
    14281 gatccgctac gggtatctgt actttctttt ttatggttta agctggttgg ggcagttgct
    14341 tcagcaaatc atgaattcga caacgaatac ggtgttgcag atgagtgcag tattcatttc
    14401 tctcggtgtc ttgttactac aaggtatttt tattttaaat atcttgctat catttttgag
    14461 aaaaaaacgg attctgtttt atgaaaaagc tagtcacaca tatactgtta gcacgattgg
    14521 ccaaacaaag gatgatgatt ctcgaaatta gctgagcaaa aacgctttgt ctgtgttata
    14581 atgccaaagt cgtgataatt taaagagaag aggtgcacga atgaataatt ttgatatatt
    14641 ccgcttcaaa ccgtttatca atgaagcgtt aaccgcaaag ggcttcaaac agcccacaga
    14701 ggtccaggaa cgtttgattc ctttaattgc aaaaggaaaa aatatcattg ggcaatctca
    14761 aactggtaca ggaaaaacac atacgttttt gttgcctttg tttgaaaaaa tcaatgcaga
    14821 aaaagaggaa gtacaagttg ttattacagc tccaagtagg gaactagctg cacagatcta
    14881 tgaagcagct acacaaatcg ctaaattttc tacaccggag atccgtgtct cgaattttgt
    14941 cggcggtaca gacaaacagc gtcaattaaa tcgtctgcat caccaacagc cacaggttgt
    15001 agtaggcacg cctggtagaa tattagacat gatgaatgaa caagcgctga agattcacac
    15061 cgcatttgca tttgttgtcg atgaagcgga tatgacattg gatatgggtt ttttaaatga
    15121 agtagaccaa attgcaagtc gattaccaga aaagctgcag tttttagttt tttctgccac
    15181 tattccagaa aaacttcgtc cattcttacg aaaatacatg gaaaatccaa tcattgaaga
    15241 aatcaaacca aaagcagtta tttctgaaga cattgaaaat tggctgattt caacaaaagg
    15301 taaagatgag aatcaaatga tttatcaact gctgacaatt ggtcatcctt atttagcaat
    15361 cgtatttgcc aatacaaaac agcatgtgga tgaaattgcc gattacttga aaaaccaagg
    15421 cttgaaagta gcaaagattc atggagacat caccccaaga gagagaaaac gagtgatgcg
    15481 acaagtacag aatctggagt atcaatatgt tgtagcgact gatttagctg ctagagggat
    15541 cgatatcgaa ggtgtctctc atgttattaa tgcagaaatt ccagaagatc tagacttctt
    15601 tatccatcgt gtcggacgta ctggacgtaa tggcttaaaa ggaacggcca ttacgttata
    15661 ttcgccaaat gatgagcaag ccattgatgc gatagaaaaa ttgggcgtct catttgaacc
    15721 aaaagaaatc aaaaacgggg aaatcgtcaa aacatacgat cgtaatagac gtgccaaacg
    15781 agaaaaatca aaagatgtat tagatcccac gctgattggt cttgtgaaga agaaaaagaa
    15841 aaagatcaag cctggttata agaaaaaaat cgattgggca attgcagaaa aaaataaaaa
    15901 agaacgcaaa atcgaacgca gacagcaaag ccgtactgct cgtaaaaata agaaaaattc
    15961 acataaataa aaaacaagag gttgtgacaa aaggcgtgtc acaatctctt gtttttaagc
    16021 tagacttttt ttcattccga tacaaagtac ttcatcttca ataaaaggtt ctgtgcaagt
    16081 atgataccct tgtttttcat aaaaatctac agccgtcatt tcagctacta cataactagt
    16141 ctgacaacct tctgaaaccg cccgtttttc agcagcttca agcaactgac cgcctaaccc
    16201 ttttcctcgg tattcttttg ctacgcaaaa tcgttccggc ttgaagcaag taggtgtttt
    16261 cttttcataa cgcaaagtag ctactggtct tttttgatca agaaatagat aataggtttg
    16321 ttgcgggata tcttttccgt cgaattctag ttctgcagga atcccttgtt ctaatacaaa
    16381 aacttgatat ctcaaaaagc agctagctgc gcgtatccat tcatccgctc caactatttt
    16441 tttcatcata ttcctccttg tcatgaatag cagagatgta ttgattcctt tattcttatc
    16501 gtaccaaaaa tcgtggagta tgaccaaaag gaaatgaaaa taaaaattga gatattgaca
    16561 gtgatcaata gaatgcgtat actaattaag tacgaaaagg atatgatttg ttttttaaga
    16621 ctcacagaaa attctttctt ggctgaaaaa agaatagtca ctaatcaaat tgctcccttt
    16681 ttctattttg gtagaaatac cgacgcagct cagcgttatc gagtttaaga ggtaatgatt
    16741 acacatcatt gcaatcaagg tggtaccgcg agttttcgtc cttggctgtg atggtgtttt
    16801 tttgtattta atttatagaa agcaggtaat gacatgaaac aattaagtag tgcacaagtt
    16861 cgacaaatgt ttcttgattt cttccaatct aaaggacata cgattgaacc tagtgcgtct
    16921 ttgatccccg tgaacgatcc aaccttattg tggatcaact ctggtgttgc aacattgaaa
    16981 aaatattttg atggttcagt tgtgccagaa aatccaagaa tcacaaatgc ccaaaaatct
    17041 attcgaacaa atgatatcga aaatgtcggt aaaacagcac ggcaccatac gatgtttgag
    17101 atgctgggta atttttcgat tggtgactat ttcaaaaatg aagccatcca ctgggcatgg
    17161 gaatttttga ctgatccaaa atggatggca ttcgatccag aaaaacttta tgtaacagtt
    17221 tatccaaaag atacagaagc aaaacgcatc tggcatgaag aagttggcct gccactagat
    17281 cacattattg atgtagaaga caacttctgg gatatcggtg caggcccatg tggtccggat
    17341 acggaaattt tctatgaccg tggagaagct tataacgatt tagcagaaga cgatcctgaa
    17401 aactatccag gaggagaaaa tgaacgttat ttagaaatct ggaacttagt attttcagaa
    17461 ttcaaccata agcctgataa cacatttgaa ccacttcctc ataaaaacat tgatacgggg
    17521 atgggattgg aacgcatggt ctcaatcgtt caagatgcgt caacgaactt tgagacagac
    17581 ttgtttatgc ctattatcca cgccgtagaa aagctaagca aacaagtaaa atatggtgaa
    17641 aacccagtaa cagatatttc ttacaaagta atcgcagatc atattcgcgc cctttcattt
    17701 gctattggcg acggtgcatt gccatcgaat gaagggagag ggtatgtctt acgccgtttg
    17761 cttcgtcgtg ctgtcatgca tgggaaaaaa ttaggtattg aacaagcatt cttatatcgt
    17821 ttagtccctg ttgttgggga aatcatggta agttattatc ctgaagtact tcaacaaaaa
    17881 gaatttattg aaaaagttat ccgtacagaa gaagaacgtt tcaatgaaac aatcaatgaa
    17941 ggtttggaaa tcttaaatca agtgatccaa gaagtaaaaa acgaaaacgg gacgactcta
    18001 gcaggaaaag acatcttcaa actatacgac acatatggct tcccggtaga attaacggaa
    18061 gaggtggcag aagatgaagg actgaaagtt gatcatgctg gttttgaaaa agaaatggat
    18121 gcacaacgcg aacgtgctcg ctctgcccgc agtactgaaa aatcaatggg ggtacaatct
    18181 gctttattga cagatatcaa agtagaaagt aaattcattg gatacacaga acaagaatca
    18241 gatagtcaat tattggtcat cgttaaaggt gagtctcttt tgaatgaagt cgcagaaggt
    18301 gaagctcaaa tgatctttga tcaaacacct ttctatgcag aaatgggtgg gcaagtagcg
    18361 gatcaaggat ggatcatgaa tgaagtaggc gaaatcgtag cagttgttac agatgttcaa
    18421 aaagcgccta atggtcaatt tatgcacacc gtagaagtaa aagcaccaat gagtgaagga
    18481 caaacctatc atctattgat cgatcaatcg atgcgcaatc ggatcatcaa aaaccataca
    18541 gcaacccatc tactgcacaa ggcattgaaa gaagtacttg gcgagcatgc aaatcaagct
    18601 ggttctttag tcgctcctgg acatttacga ttcgacttta cccactttgg tcaaatcact
    18661 tcagatgaat tagctgaaat ggaaagaatc gtcaacgaaa aaatctggga agctatccca
    18721 gttgaaacaa ttgaaacaga tatcgataca gccaaaaaca tgggtgcaat ggctttgttt
    18781 ggtgaaaaat acggacatga cgttcgagta gtaaatattg gcggctggtc aatcgaactg
    18841 tgcggaggca cacatgtagc aaatacagaa aacatcggaa tcttcaaaat cgtttctgaa
    18901 tcaggaattg gtgcaggcgt gcgacgaatc gaggctgtaa caagtaaaga agcgtacgaa
    18961 ctacttgaag aagaagaaaa acaactaaaa caagtagcaa caattgttaa atcacctcaa
    19021 ttgaaagaag ttgtttcaaa agcggaacaa ttgcaacagc aagtaagaga gctacaaaag
    19081 gaaaatgaac aattagctag caagttagcg aatcaacaag ctggtgatgt attcaaagat
    19141 ccagaaacga tcaatggaaa aacaatcatt gcagcacaag taaaagtgaa agatatgaat
    19201 cagctgcgtc aattagctga ccaatggaaa caaaaaaata tttcagatat tcttgtttta
    19261 gcaaacgaac aagaaggaaa agtcagtctg ctagcggcaa tgacgaaaga atcaaatgaa
    19321 gctggactaa aagctggcga cttgattaaa gcaatcgcac cgaaagtagg cggtggcggt
    19381 ggtggacgtc cagacatggc acaagctggc gggaaaaatc ctgctggtat tccagatgca
    19441 cttaaagctg cgaaagaatg ggttgcgcaa caatagggcg tttacgtttg acagaaaaac
    19501 aaaagttcaa acattgaacg aaacatgatt atgggggcga agacaaacaa gtcttgccct
    19561 catacttttt tcaattggat ggcgcatcta atcaaatggt cggattcgga tggtctttag
    19621 ttgacgaaag tgacagaaat ttttacgatt agtataaata aaaaggggtg aaaaaatgaa
    19681 tagtagaaat aagacctttc gactagtctt acgtgcgatc ttgttagcaa tcatcatcgt
    19741 acaagcgatg gttccttggc tggggtttat tccattagga ttcattagtt taacaattat
    19801 ccatatcaca gtaattattg ctgcagtagt attaggccca aaagatggca tggtgattgg
    19861 cttgttttgg ggaatcgcta caattgttcg tgcctatgcc atgccaacaa cgccatttga
    19921 tacattagtg ttcacgaatc cgattatctc ggtagttccc agagtacttg ttggcttagt
    19981 agcaggcttg gttttccact ggatttatca aaaaaataaa tcaataacga tcagctcagc
    20041 ctttgcaggg gtactgggtt cattggtcaa tacagtctta gtgttaggat tcatgggatt
    20101 attttataca ggtgcaacgg cagatgccta tggtgtagac tctgctcttt tatttaaaac
    20161 cttagcaggg gttgcagcaa tcaatgggat accagaagca ataggtgcgg gaatcataac
    20221 accattacta gcaaaagcgt tatttgcagc aacaccgttg aaaccagaat aatgaccatc
    20281 ttttaatagt aataggacgc acgatagata gaaaaaggaa gagaggagag gaaaacaatc
    20341 gtcctctcca tctttttacc tgagcaacaa gcaaactaga aggggtaaaa aacttggcta
    20401 agaaacaaga agaaaaaaag aaacaaatac cgcaacagcg gacaaaaaaa ggagtactag
    20461 cgggtatctt aggattggtt acgaatctga tcttgtttgt ctctaagcta atgatcgggc
    20521 ttttttcagg aagtgtctcc attatggctg atgcagtgaa cagtctatca gatactgctt
    20581 catccatatt gactttgatc ggatttaaaa ttgctgcaaa gccagcagat caggaacatc
    20641 cttatggaca cgaacgcttc gaatatatca gtgggttatt tgtttcgatc atcattactt
    20701 atgtaggatt tcaattttta gagtcttctg ctgaaaaaat catccatcca gaaaatgtct
    20761 ctttaacacc catcgtcttt tttgtcctaa tcatttccat ctttttgaaa tttgcacaag
    20821 gcaaaatgta tcaggttatt gcgcaatcca ttgattctga aacattgaga gctacagcaa
    20881 cagatagtta taatgatgtc tttactacac tggctgtatt gctttcagca gcaatcgaat
    20941 gggtaactgg atggcgtatt gatggatata taggttttgc tttagcatta tatatccttt
    21001 atagtggatt gacgatgctt cgaaattttg tatacgaact attaggaagt cgcccaacgc
    21061 cagcagagat tgaagaaatg gaagagcatt taaatgcttg cgatgatata cttggctatc
    21121 atgatttatt ggtgcataac tatggaccca ataaacgctt tgcatcagtc cacatcgaag
    21181 tagatgaatc aatgaatttg aatgtggctc ataagattat tgatctgata gaaaaagact
    21241 ttaaaaagtc tttggatgtg gaacttgtct gtcacttgga tccaatgcct gttgataacg
    21301 aaaaatacta taatatcttg cggcagttaa agcaaattct tcttgaaaca ggtacagggc
    21361 ttaatattca tgattttaga gtaattcatc aaggaaaaac gctgcaattt gatgtagtgg
    21421 tccctgagaa attccagttt gttgatacag aactagaaag aatcttacgt agtcaaatag
    21481 aagaaaaaat cggagattac caattggaaa ttacgtttga tcacaattat cttttatatt
    21541 aaatgagaaa ctataaaaag actccttaaa gtttttaatt taaagagtct ttttttcttt
    21601 taattgttta tggagagcct gacgtgcaag attatctgct cctttatttt tgttttcggg
    21661 caaccattgc aggatcaata aagaaaattc ggatagaagt tcttggattc tttgaaggta
    21721 gggaaggaag agctcgttct tcgttgcgtt tttgtcgacc gtagaaacta gtgttttgct
    21781 gtctgaaaaa cagaaaatcg tttcattctg taagtcgttt ttctttagaa attctaaagt
    21841 ttttaaaaag acttcgaact ctgcttgatg gtttgtccct atagaaagcg ggatagatag
    21901 ctgttcttgt ttaccttgat agacaatcaa gattccacct ccactaggac cagggttgcc
    21961 ttttgtagag gcatcaatgt atacttttaa cataaaaaga gattctcctt tttgttcaaa
    22021 ctaatgttat ggtaagatta tatcattact gaatcggtga gaggttagag aatgaaaaaa
    22081 aatattcgat ggcagcctga actggcacat acgatcatct attggtcatt gaccttgatc
    22141 attctgtttt ttagtctgat tctttcttta gaaaacacag gtccttattg gaaaagcaat
    22201 tttgttttag gtatttttct tgtttttcta tttctaggtt tgcggcgttc ttttatttta
    22261 aaatcaaact ctgtgattat acgttatgct gctttttgga aagaccgaga aatatctgtc
    22321 ggacagattc gatctttgac gccaactaat actggggtgc aaatgatgtt gcaagatgag
    22381 aaagccccat ctgttttcat gatgcgaaaa aaagatcaag tagtcctttt tgatcatttt
    22441 gaaaaaaacg gacccaaatc agaatcaatt ccttatcctt atacgaaaaa aagaaaaagc
    22501 taattttaaa aatcagaacc taaaaaaacg aatttttaca ggaaaagaaa agagtgagac
    22561 gtacggttca tcccgtttag cgtctcactc tatttgttta tctattcatg catactagta
    22621 tcggtagtct gaacgttgcc tttcttgaca tgggcagctt gtgttcctcg attcccttcg
    22681 atgatctcaa attcgaccac ttgattttct tccaaagttt tgaatccctc ttcttcaatc
    22741 gcagtaaaat gtacaaaaat ttcttctgtg tcatcataac tgataaagcc gtatcctttt
    22801 ttgttgtcaa accattttac aattcctgtg gtcaaagcac tcacgacctt tctaaagtct
    22861 gatcatagcc ttcattataa agagtattta taagtacgtc aaataaatta aaaacctgta
    22921 attttatgtt ttttttctga ataaaaaaag gtataattgg catatcttaa caaagaagat
    22981 attttgttga ataaagttcg tgaaaagggg taatcaaatg aagaatgatc tgcaaatttc
    23041 acaagaaaca gagctagaac cgattgttga agtggctagt aaattagatc ttaaggaaga
    23101 cgatttggaa ctgtatggga aatataaggc gaaaatcaat ttttctggaa tcaatcgatc
    23161 aaaggataac gctgaggggc atttgatctt agttacttcc atcaatccaa caccagctgg
    23221 cgaaggaaag tcaacgatca ctgtagggtt aggagacgcg ctcaataaaa tcaataaaaa
    23281 atctgttatt gcattgcgag aaccctcact tggtcctgtg atgggaatca aaggtggtgc
    23341 tgcaggcgga ggatatgcac aggtattgcc tatggaagat atcaacttgc attttactgg
    23401 tgacatgcat gctatcacaa cagctaataa tgcattatct gctcttttag ataatcatat
    23461 tcatcaagga aatgaattga acatcgatgc ccgccgagtg atttggaaac gggtagtcga
    23521 tctcaatgat cgtgaactgc gtaaagtaat tgttggcttg ggtgggccaa tccaaggtgt
    23581 tccaagagaa gatgggtttg atatcacagt tgccagcgaa atcatggcta tcttgtgtct
    23641 tgcggcagac cttgaagatt taaaggctcg tcttgcaagg atcgttgtcg gctatacatt
    23701 tgaccgtcag cctgtgactg ctggagactt gaaagcagaa ggcgctctgg cattactgct
    23761 gaaagatgca atcaagccga atcttgtaca aacaatttac ggaacaccag cttttgtcca
    23821 cgggggaccg tttgccaata tcgctcatgg ctgcaatagt attttagcta ccaagacagc
    23881 attgagatta gcagattacg ttgtcacaga ggcaggattt ggtgcagatc ttggcggaga
    23941 gaaattttta gatatcaaag tacctaactt gaaaaaagcg ccggatgctg tggtgattgt
    24001 cgccactgtc cgtgcattga aaatgcatgg cggaatgaaa aaagatgaac ttaaaaatga
    24061 gaatcttgat gctttgaaag taggttttgc aaatttaaag cgccatatac gtaatatgga
    24121 acaatatcag ctacctgtga tcgtcgcaat caatgaattc gtaacagata cagacaatga
    24181 actcacactg ttggagcact tgtgtgaaaa tcaggggatt cttgccaaga gagcgagtgt
    24241 atgggcagat ggagcagaag gcggcgttga tttagcaaaa gctgtagtac gattgattga
    24301 tagaaaagaa gctaattaca agccattata ccgcctagaa gaaacgatac aagaaaagac
    24361 agaaacaatc gtcaagaaaa tttacggagg aaatggtgtt gttttcagca aaaaagcgca
    24421 aaaacaaata gaagagttta cgaagaatgg atgggacaaa ctgccgattt gtatggcaaa
    24481 aacacagtat tcattttctg atgatccttc actgctaggt gcaccggaag atttcaccat
    24541 cacgatccgc gaaatcatcc caaaattagg ggcaggattt ttagtagcct tgactggaga
    24601 tgttatgacg atgcctggtt tgcctaaaaa acctgctgca ttgaatatgg acgtgacaaa
    24661 tgacggagaa gtgttgggat tgttttaagc ttgattataa tctaccatcg aaggagctgt
    24721 attttgacta cagcttcttt ttttagacga tagggaatta attttgttta cactaggatc
    24781 attctttttc tttattgtat tttgagctat actaaagcat aataggaggg tatgatgaaa
    24841 acgacattta taagaaaaac cggtttttac ggtatgggta gctcgattca gctaagaatc
    24901 aacaatgaaa aacgcagtgt tctcagtcat gaacaaaaga taacgcttga tctggaagtg
    24961 ccctttacga tgcaggtcac cttttattgg ctaaaaagcc ccgtttatac agtgaaagaa
    25021 gcaaagcctg cttatgtgat cacaatgaat tacttgattc ttcaaatcta tccgttttta
    25081 tttctagcaa ctgggttggg ggccgttttc ctacaaaatt tatttttcag tttttttgca
    25141 gttattgtgt tgattgcgtt ctttttctat ataaaaaacc gggcatattt gatcaaggag
    25201 gaaaattatg gcaaactctg atcttttttt aatcagtttc aatcgaatcg aaaaatggat
    25261 gagagacgag atgggaaatg cacgaaatat gggatttacc gaacttgtca gacgcctagc
    25321 tcaaagaaaa caattaatga tcagaaaata cgaagatgat cttttacagc ttgctcagtt
    25381 aaggaatgcg atcgtccatg atcggatcgc agtcgatttt gtcattgcag agccaaatga
    25441 ttggacagtc aagcgaatcc agctgatcga acaagaactg attcagcctg aaaccgtgct
    25501 gcctcgattt gcaaaacgtg taacaggttt tgaacaagat cttccgttac ttgaactgtt
    25561 aaagatagta gcagaaaaaa ggtattcaca atttccgtta tacaataaag gaaaatttgt
    25621 agcattgatt actttacgta atatcgggtt ttggctagca aaagaaagcc aaaaaggtcc
    25681 ggttgattta acaaataaac gagcgttgga tttgatcatc caaaacggaa agtacacaaa
    25741 ttatcatttt gttccagcta aaacacatat ttacgaggtt gaagcgatgt ttcgtgagca
    25801 agggactttg gaagccgtac ttatcactaa agatgggaat cccgatggta atctgctcgg
    25861 gatcgtacga cctcgtgata tttataaaca aatagaaaag gattgatttt ttgttaatta
    25921 tttattggat attcagcgct ttgctagtag gagtggatca gttggtcaaa tggtggatca
    25981 ctgataatct agcgcttgga gagacgaaaa atcttatacc aggtattttg tcactgaatc
    26041 atattcgaaa cactggagcc gcatggagta tgctagaagg gaagatgtgg ttctttacta
    26101 tagtgacact cattgcagtc gttgtgattc tcacattgat gattaaaaat cgtgaaaaag
    26161 gtaatcgctg gttcatgatt ggattgagtt tgattctagc tggtgcgatt ggcaatttta
    26221 tcgaccgtat tcgtcttggc tatgtagtag atatgttcca gacagatttt atgagctttc
    26281 cgatttttaa cgtagcagat gtgacacttg tatgtggagt gatttgtatg ttaatttata
    26341 ttattttaga tgaaaaggat caaagaaaaa aatgacagaa cttaaaacga cgattcacca
    26401 tgaatctggt aggatcgata aagtattgac taatttattc accgattaca gtcgttcgca
    26461 aattcagctg tggctgaaaa atggagcggt ttctgtcaat ggacagccag tcaaagcaaa
    26521 ttacaaagta aaacaaggag acgagattac tgtcttgatc cccgaaccag aaacattaga
    26581 tgtggtccca gaaaatatcc cgttgacgat cgtttacgaa gatgaagatg tagcggtgat
    26641 aaacaaacct caaggaatgg tcgttcatcc ttcagcaggt catacgcatg gcacaatggt
    26701 caatgcgctg atgtaccata tcaaggatct ttctacgatc aacggcgtta ttcgtccagg
    26761 tattgttcac cgaatcgaca aagatacttc cggactgcta atggttgcca aaaatgacca
    26821 ggcacacgaa tcattagctc agcaattaaa agataagacc tctttgcgca aatatgttgc
    26881 tttagtccat ggagagattc ctcacgaaaa aggaaccatc aatgcgccaa tcggccgttc
    26941 gaaggaaaac cggaaaatgc aggcgataag agaagatggg aagtcggctg tcacacattt
    27001 tactgtattg gaacgtttcg aaggatttac tctggttgaa ttaaaactag aaaccggaag
    27061 aacacaccag atccgtgtgc atatgaagta tatcggttat ccgttagctg gagatccagt
    27121 atacggaccg aagaaaactt tgaaaggcaa tggtcagttt ctccatgcgc aaatgttagg
    27181 ttttacgcac ccgactacaa acgaaaagat gatctttgaa gcacctcttc cagaagtttt
    27241 tgaaaaaact ttagaaaaat taagaaaaaa cattgcattt taatttagat aacggtatag
    27301 taattaacaa cgaaagcata aaacttaata gaattccttt aagtttagtc ccgtgaggct
    27361 aagaagggtg ggagcaagga tacgtatggt gcgccataag tagattgctc attgacatag
    27421 tatagctatc agtgtatcag tatacgctca gtctaaacat tcctcctgtc ctcttagggc
    27481 aggagttttt ttatgcaaaa aagtaggggg aatgaaaatg caagcaaaag aagtagtaga
    27541 tcaggtaacc atgaagcgag cattgactcg aattacttat gagattatcg agagaaacca
    27601 tagtatccaa gatatcgtat tggtaggtat caaaacacga ggaatctaca ttgcatcaag
    27661 aattgccgaa cgcctgaagc agctagaaga tatcgatatt cctgtaggag aactggatat
    27721 cactttatac cgagatgaca aaaaagaaaa tccagaagaa ccggaacttc attcttctga
    27781 catcccagtt tccttagaag gaaaagaagt tattttgatt gatgatgttt tgtatacagg
    27841 acgaacgatt cgagcagcaa tggacgcagt aatggatttt ggcagaccaa gaaaaatttc
    27901 attagcagtc ctggtcgatc gtgggcatcg agaattgcca atccgtgcag attatgttgg
    27961 gaagaacatt ccaacatctc gggcggaaga gattttagta gagatgcaag agcttgatgg
    28021 acaagatcgc atcatgattt tgaaagagga agattagagg agagagaaac atgtcggaga
    28081 aaaaaggatt tcataatgat gacgtggtgt tagatatcca cgatcgccca accccaggac
    28141 attgggtagg attaagttta caacacttgt tcacgatgtt cggagcaacc gtgttagtcc
    28201 ccatcttagt aggtatcgat ccaggtattg cgctagtcag ttcaggacta ggtacattgg
    28261 tgtacttatt tactactaaa ggaaagatac ctgcgtactt aggaagcagc ttcgctttca
    28321 ttgcagcgat gcagcttttg atgaaaacag atggttatcc agcgattgca caaggagcag
    28381 tcacaactgg attagtctat ttgattgttg cgctgatcat caaaaagatc ggttcagcat
    28441 ggctggataa aatcttgcct ccaatcgtcg tcggtccagt catcatggtt atcgggttgg
    28501 gacttgcggc aaatgcagca acaaatgcta tgtataatca gggcacgtat gatttcaagt
    28561 atattgctgt agcgctgatc acattaggat taacaatttt cttcaacatg tggctaagag
    28621 gttttctcgg attgattccg attttactag gtattattag cggatacatc gtagccttgc
    28681 tgtttggaat cgtggatact aagccaatcg tagacgctgc ctggtttgct gttcctaact
    28741 ttgagattcc ctttgtacaa tatgaaccaa agctatatct tggagcaatc acaacaatgg
    28801 cacccatcgc ttttgtgacg atgacagagc atatcggcca tcttatggta ttgaataaac
    28861 taactaaacg taatttcttt gaagaaccgg gtcttcataa gacgttgaca ggtgatggat
    28921 tagcacaaat cgtggcagcc tttgtcggag gtcctcctgt aacaagttac ggtgaaaaca
    28981 ttggtgttct ggctatcaca cgtgtccata gcgtgtttgt gatcggtgga gctgcagtat
    29041 ttgcagtcat tttaggattc gtaggaaaat taagcgcttt gattttaagt atccctggac
    29101 cagtcatctc aggaatcagt tttatcttat ttggagtaat tgccgcaagt ggaatgaaga
    29161 ttttaatcga gaacaaaatc gattttgata aaaagaaaaa tctgttgatt gcttcagtta
    29221 tcttagtagt aggaattggc ggactcgtct ttgaagtagg cacatttaca ttgtcggcaa
    29281 tggcattagc aactgtactt ggcatcattt taaatcttat cttaccagaa acttcacgaa
    29341 gcgaagaaaa ctaaggagga aacattatga tcatcaaatc agaacgtatc agcttaaagc
    29401 atttattgac cgttgaggca cttactgatc aagaagttat gggcttgatc cgcagagggc
    29461 aggaattcaa gcaaggtgct aattggactc cgcaaaagcg ccagtacttt gctacaaacc
    29521 tctttttcga aaatagtact cgcacacata aaagcttcga tgtagcggag aaaaaactag
    29581 ggctagaagt gattgaattt gaagcaagca caagctctgt ccaaaaaggg gaaacactat
    29641 acgatacagt actgacgatg tcagcattag gcgtagacgt agcggttatc cgtcatggtg
    29701 atgagaacta ttatgacgaa ctgatacaaa gtaaaacaat ccagtgttcg attatcaatg
    29761 gaggagacgg aagcggacag catccaaccc aatgcttgct tgatttgatg accatttatg
    29821 aagaatttgg tcattttgaa ggtctgaaag ttgccatcgt aggtgatatc actcactctc
    29881 gcgtagctaa atccaatatg cagatgttaa aacgtctagg cgcacaagta ttcttctcag
    29941 gtcctagaga atggtacgac gatgaattcg aagtgtacgg acattatatg ccattggatg
    30001 aactactaga tcaagttgat gtcatgatga tgcttcgggt gcaacacgaa cgccatgatg
    30061 gaaaagaaag tttctcaaaa gaaggctacc atcaggaaca tgggttaaca gttgagcgtg
    30121 cgaagaagtt gcaaaaacat gcaatcatta tgcacccagc accagttaac cgtgatgtag
    30181 aattagctga ttcattagtg gaaggattac agtcgcggat cgttcagcaa atgagcaatg
    30241 gagtattcgt cagaatggca atcttagaag cagtgctaaa tggaaaagcg taattatagg
    30301 aggatgaaaa aatgaaaaca ctcattaaaa acggtcagat caatacacga aaaaatatga
    30361 cgacaccagc agaaatttgg atagaagatg ggcgaatcaa agcaatcggt acaggattct
    30421 cagaagccga atttgatgaa gtgtttgatg caaagggaca attgatcaca cctggtttag
    30481 tggacgtcca tgttcatttg agagagccag gatttacata caaggagacg atcgaagctg
    30541 ggactcgctc agctgctcgt ggcggtttca ctactgtatg cgcgatgccg aatttaaatc
    30601 ctgtacctga tacggcagaa aagctgagac aagtatatga catcattaga aaagatgcag
    30661 ttgtgaaggt attgcagtat gcgccaatca cagaaaatct gcgaagtgag aaattagttg
    30721 atcaagaagc actgattgaa gaaggtgctt ttgctttcac aaatgacggt gtaggtgtcc
    30781 aaacagctgg gacgatgtat ctggctatga aagaagctgc aaaaaacaat aaagccctag
    30841 tagcccatac agaagatgaa tctttattat ttggcggcgt tatgcacgct ggcaagaagg
    30901 cagaagaact tggtcttccc gggatcttaa gcgtaaccga gtcttctcaa attgctagag
    30961 atctgttgct agctgaagca actggtgttc attaccatgt ttgccatgtt tctactaaag
    31021 aaagtgtccg tgtaatcaga gacgcaaaaa aagcaggaat ccacgtaaca gcagaagttt
    31081 caccccatca tcttattttg atcgacgaag atattcccga agatttcggc ttttggaaaa
    31141 tgaacccgcc gctaagagga cgggaggaca gagaagcttt gatcgaaggc ttgcttgacg
    31201 gtacgatcga ttgcatcgct acagaccatg ccccccatgg attagaagaa aaaagccaaa
    31261 gtttcatgaa atcaccattt ggtatcgtag gaagcgaaac agcgtttcaa ctgatctata
    31321 ctcattttgt agaaactggt cgattcacgt tggaacaagt gatcaattgg ttggcagtta
    31381 aaccggctga gatttttggt ttgaatgcag gaacgctaac ggttggtgcc cccgctgatg
    31441 tagcagtttt tgatattaca caaacttgca cgatcgataa agaagacttt ttatcaaaag
    31501 gggagaatac accttttatc ggctggaaag taaaaggcga aacacaaatg acttttgtca
    31561 acggaaaact tgtgtggcag aagggagaat aaaagtatgg aacgtttgct tatattagaa
    31621 gatggaacag ttttcgaagg aaaggctttt ggtgcagatg ctagtgtctt cggagaaatc
    31681 gtttttacaa caagtatgac tggttatcaa gaaactatca ctgatcaaag ttttaacggt
    31741 caaatcatta catttaccta tcctatggtg ggtaattatg gggtaaatag agatgattat
    31801 gaatcgattt ctccaacatg caaaggagta gtagtaaaag aacatgcacg tgtagcttca
    31861 aactggcgaa accaaatgac gctagattca tttttgaaac aaaaaggaat tcctggaata
    31921 tcaggtatcg atacgcgcgc ccttactcgc aaaatccgtg aagtcggaac gatgaaagct
    31981 agtatagttg atgctggaga ttccttcgaa catgcatttg atcagctgaa agcaaccgtt
    32041 ctagcaacca atcaagtgca gcaagtctca actaacaaac cttatccaag tccaggaaca
    32101 ggtcggaaag tcattgtcat tgacttcggc ttgaaacata gtattttacg agaattgagt
    32161 aaaagaaact gtaacctcac agtacttccc tataacacag atgccgagac gattttagcg
    32221 ctatcaccag acggagtcat gctgacgaat ggacctggtg atccaaaaga tgtgccagaa
    32281 gcaatcgaaa tgatcaaaaa aatccaagga aaagtaccga tttttggtat ttgtctcggt
    32341 caccagttat ttgctttagc caacggtgca gatacctata aaatgaaatt cggacatcga
    32401 ggattaaatc acccggtacg agaagtagcg accggaagaa ttgattttac ttctcaaaat
    32461 catggctatg cagtcgatga agccacgatt gatccagaaa aactcatcgt aactcatgta
    32521 gaagtaaatg acggaacagt agaaggagtc aaacacagag attatcccgc tttcagtgtg
    32581 cagtttcatc cagatgctgc ccctggtccg catgatgcac tacatttatt tgatgaattt
    32641 atggaaatga tggacgcagg aaaggagcag taaacagatg ccgaaacgta cagatatcaa
    32701 gaaaattatg gtgattggat ctggtccgat catcattggt caagccgcag aatttgacta
    32761 cgcaggaaca caagcctgcc ttgccttaaa agaagaagga tatgaagtgg ttttagtaaa
    32821 ttctaatcct gcgacgatca tgacggataa agaagtggca gatcaagtat atatcgaacc
    32881 gatcacactt gaatttgtat caaggattct tcgaaaagag cgtccagatg ctctccttcc
    32941 aactttaggc ggacagacag gcctcaatat ggcaatggag ctggcagcat caggtatttt
    33001 agaagaattg aacatagaac ttctaggaac aaagctttct gcgatcgatc aagcagaaga
    33061 tcgtgatcta ttcaaacagt tgatgaaaga attgaatcag ccaatccccg agtctgaaat
    33121 cgtcaataca gtagaagaag ctgtagattt tgcaaataaa atcggctatc ctgtcatcgt
    33181 tcgtccagcc tttactttag gcggaactgg tggaggtatg tgtgaaaatg aagaagaatt
    33241 acgtatcgta tcagaaaatg gattaagtct ttcccccgct acccaatgtt tgattgaacg
    33301 aagtattgca ggttttaaag aaattgaata tgaagtgatg agagattcgg ctgataatgc
    33361 aatcgttgtc tgcaatatgg aaaattttga cccggtgggt atccatacag gtgattctat
    33421 cgtatttgcc cctagtcaaa cattgtctga ccatgaatat caaatgcttc gtgatgcttc
    33481 tctttcgatt attcgagcac ttaagatcga gggaggctgt aatgtccaac tcgctcttga
    33541 tccacatagt tttaattatt atgtgattga agtcaatccg cgtgtttctc gttcatctgc
    33601 gttagcaagt aaggcgactg gctatccgat cgctaagcta gctgctaaaa tagcgcttgg
    33661 attaacatta gacgaaatga aaaatcctgt tacaggaacg acttttgcag aatttgaacc
    33721 tgcattagat tatgttgtag ctaaaattcc tcgctggccg tttgataagt ttgaaaacgg
    33781 agaacggctt ttaggcacgc agatgaaagc aactggtgaa gtgatggcta tcggtcggaa
    33841 tatcgaagag tctttattga aagctgtgcg ttctttagaa atcggtacac accatatcga
    33901 acttcctgag ttggcagaca tcacggacga tgacctaatc caaaaagtgg ttcgagcaca
    33961 agatgatcgc ctgttttatg tagctgaagc tattagaaga gggtacacca tagaagaatt
    34021 agcggatttg acaaaaatcg atttattctt tttagataaa ctgttgcata tcgtagaaat
    34081 cgaacagcag ttgaaacagc agccgttgga tgacaagata ttgaaaacag cgaaacaaaa
    34141 cggttttaca gacaaaaaag tggcggagct gtgggaaata aacgaagaag ccgttcgcca
    34201 gatgagaaaa gaaaatgatt tgattcctgt atataagatg gttgacactt gtgcagcgga
    34261 attcgaatca aaaacacctt atttttatag ttcctatgaa tatgaaaatg aaagttcacg
    34321 atcagaaaag ccctcaattc tagtactggg atctggtcct atccgtatcg gacaaggggt
    34381 tgaatttgac tatgcgactg tgcattctgt caaagcaatc cagcaggcag gatacgaagc
    34441 aatcatcatg aacagcaatc ctgaaacagt ttcgactgac ttttctattt cggataaatt
    34501 atattttgag ccattgacct tcgaagatgt gatgaatgtg atcgagctag aacagccgat
    34561 aggagtaatc gttcaatttg gtggtcagac agcaatcaac ttagccgaac ctttgactgc
    34621 tgctggtgta aaaatcttag ggacaacgat cgaagacttg gatcgtgcgg aaaatcgtga
    34681 tttattcgag caagcgctga aagagttgga tattcctcaa cctttagggg atactgctac
    34741 aagtaaagaa gaagccgtgg ttatcgcttc gagaattggt tatccagttc ttgttcgtcc
    34801 aagctatgta ttaggcggac gtgcgatgga aatcgttgaa aaccagcgag atttagaaga
    34861 ttacatggaa catgctgtga aagcatctcc agaacatcca gtactagtgg accgttactt
    34921 gcttggtggt gaatgcgaag tggatgcgat ttgtgacggt gaaacagtac tcatcccagg
    34981 gatcatggag catattgagc gtgctggtgt ccattcaggg gattcaatgg ctgtgtatcc
    35041 acctcaaaca ttatctgaat cgatcaagaa aacgatcgaa gattacacga tccgcttagc
    35101 attaggtttg aattgtgtcg ggatgatgaa tatccagttt gtcatccatg aaaatgaggt
    35161 ttatgtgatc gaagtcaatc ctcgagcaag tcggactgtt ccattcttaa gtaaggtgac
    35221 aggaattcca atggcacaaa ttgcgacgaa agctattttg ggagagaaac taaagacact
    35281 tggttatgaa aacggacttt atccagaaac aaaagaagtt catgttaaag cacctgtctt
    35341 ttcttttacg aaattgcaga aagttgatac ttacttaggg cctgaaatga aatcgactgg
    35401 agaagtaatg ggatctgaca gacaattaga caaagctttg tacaaggcat tcgaagcttc
    35461 tggattacac ttgcctgaat atggagccgt attatttacg attgcggacg aaacaaaaga
    35521 agaagcacta gaattagcta aacgttttac agaaattggc tatagtctga taggaacgaa
    35581 acatacagct gcttatttcg aaaaaaatgg tctggtcgtt gaaacagtag cgaaaatttc
    35641 tgaggaagca gcagaaaaaa atgtagtcga cttaataaga gatggtaaaa cacaagtggt
    35701 tgtcaatacg attgataaag atcggggaaa tgcttctaaa gacggtttca tcatccgaag
    35761 agaagcagtt gaacatggta ttccattgtt tacttcttta gacacagcag atgcgatcgt
    35821 tcgagtgatg gaatcccgtg ctttttcaac taaccctatt taacggcgag cacatgttaa
    35881 gacaaaaaga cgaaaaaccg ttgcaggagg ggacagatga aacaagaaat gatggaaatc
    35941 gttcagcaaa aacagctggc accaaagatt tttgaaatga cattgaaagg tgatctagta
    36001 aaagaaatgc agcaaccagg acagttcttg catattctcg tgccgcgtgc agacttgcta
    36061 ttacgccgac cgattagctt gaatcatatc gaccacgaaa caaatacttg ccgtatcatt
    36121 tatcgggtgg aaggtgaggg gactagagtc ttctctactc tttctgccgg agaccattta
    36181 gatgtcatgg gtcctttagg caatggtttt gatttgtcca atcttcatac aggagacgaa
    36241 gtatttataa tcggtggagg aatcggtgtc cctcctctat atgagttatc aaaacagtta
    36301 gtcgaaaaag gaattaagcc aacgcatttt ttaggttttg ccacacatga agtcgtttat
    36361 tatgaaaaag aatttaatcg tctaggagaa acaagaatcg ctactgacga tggaacatac
    36421 ggtatgcacg gaaatgtcgg taatttattg ttaggaacga agacggaacc tacagctgtt
    36481 tttgcatgtg gttcaaacgg gttattgaag acagtggaac agctgttttc ttcgcatcaa
    36541 aatgtccagt tatcattaga atcccgcatg gcatgtggga taggggcttg ctatgcttgc
    36601 gtttgtcata cgccggaaga tgaaacagga acaaaaagtg tgaaagtttg tgacgaagga
    36661 ccgattttta aaataggaga ggtaattata tgaatgagaa tcctttagcg gtcaaacttc
    36721 ctggcctaga attaaaaaat cctatcatgc cggcaagcgg ctgtttcggt tttggcaaag
    36781 aatacggaaa atactatgat ttgaatcagc ttggatcgat catggtcaag gcaacgactc
    36841 caaatgcccg ctttggcaat cccacgccaa gagtggcgga aacaccatct ggcatgttga
    36901 atgcaattgg tttgcaaaat cctggtctag acgttgttat gcataaactt cttccagaat
    36961 tagaggagta caatgagtta cccatcatcg caaatgttgc tggtgcttgt gaagaagact
    37021 atgtagaagt ttgtagcaaa attggcgaag caccaaatgt gaaagcaatc gagctgaaca
    37081 tttcttgccc aaatgtcaaa catggcggaa ttgcttttgg aacagattct gatgttgctt
    37141 ttcaattaac acaagcagta aagaaagttt cttctgttcc tgtctatgtc aaattgtccc
    37201 caaatgtaac agacatcgtt ccaatcgctc aggcaatcga agctggaggg gcagatgggt
    37261 ttacgatgat caatactctt cttggtatgc ggattgacct taagacaaga aaaccgattt
    37321 tagccaatca aacaggtgga ctctccggac cagcaatcaa gcctgtcgct attcgtttga
    37381 ttcaccaagt agcaagtata tccagccttc caatcattgg gatgggcggc gtgcagacag
    37441 tggatgatgt tcttgagatg tttatggctg gagcaagtgc tgtagctata gggaccgcga
    37501 atttcaccga tccgtatatc tgtcctaaac taattaaaga gctccctgtg cgaatgagcg
    37561 aactaggcat cgaatcgctg gaaagattaa tcaaagaagt gagagaggaa cgtgaacgat
    37621 gaatcaacta gaccaacgac caattattgc attagatttt tcaactaggc aagaggtaga
    37681 agactttctt cgatttttcc cgaaagaaga aaaattgttt gtgaaaatcg gaatggaatt
    37741 gttctaccaa gaaggacctg aaatcgtacg ttatctaaaa gaggctggtc ataatgtatt
    37801 tttagatctg aaacttcacg atattccgaa tacggtcgaa aaagcaatgc ggggattggc
    37861 aaagcttggt gttgatctca cctgtgttca tgccgctggc ggtattcgta tgatggaggc
    37921 ggcaatgcga ggattagaag aggggactcc agaaggtgga aaacgtccgc tacttctagc
    37981 tatcactcag cttacttcta caagtgaaga agaaatgcat gccgatcaac taatcgaagt
    38041 ttctttagaa aaaagtgtga tccattatgc tagctgtgcc aaaaaagcag gattagatgg
    38101 tgtggtttct tctgcttggg aagtagaagc gatcaaagaa atggcaggag acgaatttgt
    38161 ttgcttaact ccgggtataa gaccagaagg aacagtcgca ggagatcaga caagagtagt
    38221 cacaccttct caggcaaaaa agatcggctc aacttttatc gttataggtc gaccaatcac
    38281 tcagtcaact aatccttata aagcatatca aacgattcaa acagaatgga gctaacctaa
    38341 aatgaatgtt gaacaaagta tcgcaaaaga cttattagaa attgaagcag tatttttaaa
    38401 tccaagtaat ccttttacat gggcaagtgg aatcaaaagc ccaatttact gtgataaccg
    38461 aatcacgatg agctatccaa aagtccgaaa agagatcgca aaaggattag caagcaaaat
    38521 caaagaagct taccctgaag tacaagtgat tgctggaact gcaacagcag gaatccctca
    38581 tgccgcatgg gtagcggaaa tactggattt gccaatggtc tatattcgaa gtaaggcgaa
    38641 agatcatggg aaaggtaacc aaatcgaagg acgtatattt gaaggtcaga agatggttgt
    38701 gatcgaggat ttgatttcta caggcggaag tgtcttggaa gcagctgaag cagcaaaaag
    38761 agaaggtgcg gatatccttg gtgttgcggc tatctttacg tatgagttgc caaaaggaaa
    38821 agcaaatttc gaaaaagccg aaatccctct tatgacgttg accaattatt ctgtattgat
    38881 tgaagcagct ttagaagatc gttatatcga tgagcaagaa ttaactctgc taaaagaatg
    38941 gaaaaaagat cctgaaaatt ggcagattga agcgcgcttg atgaagatag agaaatccga
    39001 tttgtgtctc cagaatcatc aattggaata gcaaaaatcc aaaaactgat tgcgagaagc
    39061 atcttttcat caggtatgaa aagaaacttc tatcgtgatc agtttttgat ttttttaaaa
    39121 ttcatttttc taagactggt tagcagaaag aagagaataa tctcttctct tttttctatt
    39181 atatatagat tttctctatt ttttggtaag ggagctgttt caaaaatggt aaagagcgga
    39241 aatgttgcga atatcagtta tttaatgata tgatattcgg aaaaagaaat ctcaaaggga
    39301 ggtaactttt atggggttga atgaatttat tttaggattg aacaatttag agacgatcac
    39361 aagggcacct ggatttttta aatatacaga gcatacagtt gcagcacatt cgtatcgtgt
    39421 agcatctatt gcgcaagttc taggagacat tgaagaagcg aatggacaac cgatcgattg
    39481 gaaactgctc tatgaaaaat cattgaatca tgactacaca gagcgtttta ttggtgatat
    39541 caaaacgccg gtgaaatacg caagcaaaga gttaagaggg atgctgcaaa cagttgaaga
    39601 aaagatgacg gacgaattca tcaaccaaga gattccagct gagttccaag aagtttatcg
    39661 ccgtcggttg ttcgaaggaa aagatgaaac cgtagaagga gaactattag ccatttcaga
    39721 taaggttgac cttctttatg aatcttttga tgaaatcatc aaaaataacc cggaaaaagt
    39781 ttataaggag atgttcattg aggcagtgac tacgattaaa agctttgaac atcggccttc
    39841 tgttcagtat ttctttaaag agatatttcc ggaactattg aaccaggaat tttatggaaa
    39901 agaggcgttt ttagaaaccg tctcaacgat tgtaaagaaa gaagaaaaat aaaaatgata
    39961 tgagatgcag ggggaagtat agttgaaaaa gaatatggat gtcgaatggg gctattctgg
    40021 agacaaaggt ccggaaaatt gggcgagttt atgtgattgg tttgcgagag gtgcggaatt
    40081 tccattgcag tcgcctattc atttgaccaa gggggaagaa accaaaatag aaggagacac
    40141 gctttccttt cattatcaaa agcaacggtt tactgacaag gaattcaaaa acacgattca
    40201 tttagtacct tttgaccaac tcagttatgt agagttcaag aaagaacgct attatttgac
    40261 agatattcat ttccacttgc cttctgaaca tatcatcaac ggaaaacagg aagatattga
    40321 atttcatttt gttcatatga acagcaaaaa agagaatcta gttgtgggtg tcatgtacca
    40381 gctaatggta caggaaggtt ggctgtataa tcaagaaaat ggggaaagtt ggaacttaga
    40441 aaaacatgag cattgggtaa atccggatgt gttctttccg gagcaaaaaa gccatttcca
    40501 ttatattggc tcattgacaa ctccgcctac cagcgggcca atccaatggt ttgtaatgga
    40561 tcatgtagga aaactgaacc aagagtttct tctccccttc gatggtcagt atgctcgacc
    40621 gaacaaccga ccaatccagc ccttgaatgg aagagaaatt tattatttcg aggagtttga
    40681 gcaatgaata ttcagcagat gaaatacgtg gtcgctgtag caaacaatgg tagtttcaga
    40741 gaagcggcaa agaaaatgtt tgtctcccag ccaagtcttt cagctggtat caaagaatta
    40801 gaaaccgagt tagggatcac gctgtttact cgaacgaatc gaggggcata tttaacagaa
    40861 gaagggcaag acttcttaaa tcgtgccgag aaaatattgg tccagcttga tacattagaa
    40921 actcactatc taacgagtga aaagagagac aatttttcga tcgctgccca gcattatgat
    40981 ttcttaggtc ctttgaccgc acagctactc gaggaattca aagacgagat caaacaattt
    41041 cggatcatgg agacgacaac agcgagagtg atcgaagaag tcaaagagca gcacagtgag
    41101 atcggcatta tttatatgaa tgagcataat caagctggta tcagaagata tttacaacaa
    41161 ggagagctaa gtggccgcag cattggaaaa ttcaaaacgc atattttttt aaggcaagga
    41221 catccattag ctgaacacgc agagatcacc aaagaagaat tgttatctta tcctcaagtt
    41281 cgttttacac aggatggaaa taatttcccg tatttttatg aagaccttat cgagatccct
    41341 gatcaagaaa cagtgattta tacgagtgat cgaggtactt tgatgaatat cgttcttgaa
    41401 actgatgctt acgcttcagg atctggtatt gtgattggag ggattcgcga gcacttgcgg
    41461 ttaatccctt tggcagaagg cgaactaaat gaattttata tcatttattc ggcgaaacgt
    41521 ccgctcagtg aaatcgctca aagatttatc aaagagctta ctcggttact ggaccagcag
    41581 agctaagaaa agcagatgag aacgctgttt attgaacacc gttgatcaaa aaagaggggt
    41641 tgtgacagaa ttttgtcaca actcctcttt ttgtagcaaa caatttataa ctaattgctg
    41701 ttgtgggaaa gtacgcaaat atgattctag aaacactcat tgattacctg ttttgtttca
    41761 tagctgtaat tttttcttct tctggtgtca cgataatcgt cttttggttt tcataaataa
    41821 cataaccagg cttggcgcca tttggttttc ggatatgctt tacctgtaca agatcaaccg
    41881 gcacttgggc agagtatcgg tattttgaaa aataggcagc taactcggca gcttctgtaa
    41941 ttgtttcatc tgagggcttg tcacttttaa tgatgacgtg cgaaccgggt atattttttg
    42001 catgaagcca atagtcggtt tttttagctg tcttcattgt cagctggtca ttttgaagat
    42061 tgtttttacc aacaagaatg agcgttccgt cagaagaaaa gtattgatca ggctgagatg
    42121 gtttcttacg tttttgtttt tttaagttct tctttttcag gtatccttct gaagtcaatt
    42181 cttctttaat tgcttcgata tccattgggc cggcaatttc cagttgagat aagacagatt
    42241 caagatattg gatttcatct ttggcttctt ggatttgttc gccaatcagc ttaacagcgt
    42301 tcttcagctt ttggtatcga tggaaatatt tctgtgcatt ttgatttggt gtcaatgccg
    42361 gatctagtgc tattttgatt ggccgatctt cttcataata attaggtaaa acaacttctt
    42421 ttgcgccgcg tggtacttgt gtcagaaaag tagtaagtag ttcgccattt ctacggtaat
    42481 tctcagcgtt ttcggattct ttgagtgttt gttcacgttt tttcaattta tttttatttc
    42541 gttttagttc attttcgatt tttcgaatca gttctccgcc ttgttgtttt gcccgatctt
    42601 tctctgcttt ttcgtgataa tagctatcta ataattgact gagtgttgga taagctgtta
    42661 cagcagatgc ctgttcggac aatacctgat aagcaatcgg tgtgaaaaaa tctttatttt
    42721 ccgtttcgta aaaagtagga ataggctgat ggctgatggc tgagaaaaat tcgtcccata
    42781 caaccatctt ttcattagga cgttccagta atcttttggt cagctcttga gcgatatcga
    42841 aaccgattcc ctgaaactga cgttggattg cttttggatc tagttcagtt tgggaaagcc
    42901 gctgaaagat tttttctttc tcggagctaa atgggttcaa gacctcttgt tttggcggag
    42961 caacatattc tacaccagga agcaaagaac gataagtgtt ttgagagctt ccaatgtgtt
    43021 tgatcgcatc cagtattttt ccagtttctc gattaactaa gacgatcgta ctatgacgtc
    43081 ccattaattc aacgatcaat atgatatttt gtagatcacc caactcgttt cgtttagcaa
    43141 agtggaaatg gatcactcga tcattttcga tttgttcgat gtcttctaaa atagcgccat
    43201 ccaaatattt tcgaagcatc atcacgaagt taggcggatt atcaggattc tgataatcga
    43261 tttgggtaat ctgtacacga gcataacttg ggtgcgcaga taataataat cgatggtttt
    43321 tgccgcgtga acgaatcacc agcacgattt cattttcata aggctgatgg attttcgaga
    43381 tccggccaga tagtaacgtt tcacgcaatt cgttgaccat cgcgtgggta aatacaccat
    43441 caaaggacat ggttcattcc tcatttcttt tcataagtat attcattata acgaaaaaaa
    43501 gaagaaaaac caatttggcc gaatagtaaa gcaaaagtct agaaaaatct tggactaatt
    43561 acataaaaaa cagtgtttct atcgttgaaa tccaaatttt taggta
//
LOCUS       DAJSAW010000022        43206 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJSAW010000022 DAJSAW010000000
VERSION     DAJSAW010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.022"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            123..707
                     /locus_tag="ONT64_001267"
     CDS             123..707
                     /locus_tag="ONT64_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2302696.1"
                     /translation="MNKLFMISGENGTGKTTLMKLFTKQLPVSNNQLFVNGIDINMID
                     NESLRRLISIATQIPYIINSSVKENILMGTKNYDKKKFSKLIELLGISKFMTKLGITL
                     DDNIYDEKILLSGGEKQKISLARALIKSASVIILDEPFRNLDNETRDNLRDYILNTKK
                     EVCYIIITHDHILDNFLDSNDEILYLNKEYDNNE"
     gene            700..1728
                     /locus_tag="ONT64_001268"
     CDS             700..1728
                     /locus_tag="ONT64_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR2302697.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            1784..2002
                     /locus_tag="ONT64_001269"
     CDS             1784..2002
                     /locus_tag="ONT64_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302698.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            2073..2888
                     /locus_tag="ONT64_001270"
     CDS             2073..2888
                     /locus_tag="ONT64_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302699.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY"
     gene            2952..3137
                     /locus_tag="ONT64_001271"
                     /pseudo
     CDS             2952..3137
                     /locus_tag="ONT64_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
     gene            3711..3974
                     /locus_tag="ONT64_001272"
     CDS             3711..3974
                     /locus_tag="ONT64_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302700.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
     gene            4308..4679
                     /locus_tag="ONT64_001273"
     CDS             4308..4679
                     /locus_tag="ONT64_001273"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR2302701.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
     gene            4859..5281
                     /locus_tag="ONT64_001274"
     CDS             4859..5281
                     /locus_tag="ONT64_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302702.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            5426..5671
                     /locus_tag="ONT64_001275"
                     /pseudo
     CDS             5426..5671
                     /locus_tag="ONT64_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5780..6736
                     /locus_tag="ONT64_001276"
     CDS             5780..6736
                     /locus_tag="ONT64_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302703.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(7572..8243)
                     /locus_tag="ONT64_001277"
     CDS             complement(7572..8243)
                     /locus_tag="ONT64_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2302704.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            8560..10191
                     /locus_tag="ONT64_001278"
     CDS             8560..10191
                     /locus_tag="ONT64_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR2302705.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            10194..11273
                     /gene="uxuA"
                     /locus_tag="ONT64_001279"
     CDS             10194..11273
                     /gene="uxuA"
                     /locus_tag="ONT64_001279"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR2302706.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            11347..12774
                     /gene="uxaC"
                     /locus_tag="ONT64_001280"
     CDS             11347..12774
                     /gene="uxaC"
                     /locus_tag="ONT64_001280"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR2302707.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            12858..13505
                     /locus_tag="ONT64_001281"
     CDS             12858..13505
                     /locus_tag="ONT64_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR2302708.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            13634..15010
                     /locus_tag="ONT64_001282"
     CDS             13634..15010
                     /locus_tag="ONT64_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2302709.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            15021..15815
                     /locus_tag="ONT64_001283"
     CDS             15021..15815
                     /locus_tag="ONT64_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR2302710.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            15828..17594
                     /locus_tag="ONT64_001284"
     CDS             15828..17594
                     /locus_tag="ONT64_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HCR2302711.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            17622..19430
                     /gene="uidA"
                     /locus_tag="ONT64_001285"
     CDS             17622..19430
                     /gene="uidA"
                     /locus_tag="ONT64_001285"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR2302712.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            19602..20003
                     /locus_tag="ONT64_001286"
     CDS             19602..20003
                     /locus_tag="ONT64_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2302713.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            19997..20242
                     /locus_tag="ONT64_001287"
     CDS             19997..20242
                     /locus_tag="ONT64_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2302714.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(20566..20787)
                     /locus_tag="ONT64_001288"
     CDS             complement(20566..20787)
                     /locus_tag="ONT64_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302715.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(20949..21021)
                     /locus_tag="ONT64_001289"
     tRNA            complement(20949..21021)
                     /locus_tag="ONT64_001289"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20986..20988),aa:Lys,seq:ctt)
     gene            21097..21327
                     /locus_tag="ONT64_001290"
     CDS             21097..21327
                     /locus_tag="ONT64_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2302716.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            21371..22540
                     /locus_tag="ONT64_001291"
     CDS             21371..22540
                     /locus_tag="ONT64_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2302717.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            23114..23683
                     /locus_tag="ONT64_001292"
     CDS             23114..23683
                     /locus_tag="ONT64_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HCR2302718.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            23670..24530
                     /locus_tag="ONT64_001293"
     CDS             23670..24530
                     /locus_tag="ONT64_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR2302719.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            24720..25424
                     /locus_tag="ONT64_001294"
     CDS             24720..25424
                     /locus_tag="ONT64_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2302720.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            25429..27264
                     /gene="walK"
                     /locus_tag="ONT64_001295"
     CDS             25429..27264
                     /gene="walK"
                     /locus_tag="ONT64_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCR2302721.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            27261..28577
                     /locus_tag="ONT64_001296"
     CDS             27261..28577
                     /locus_tag="ONT64_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302722.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            28578..29447
                     /locus_tag="ONT64_001297"
     CDS             28578..29447
                     /locus_tag="ONT64_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCR2302723.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            29502..30311
                     /locus_tag="ONT64_001298"
     CDS             29502..30311
                     /locus_tag="ONT64_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR2302724.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(30414..31043)
                     /locus_tag="ONT64_001299"
     CDS             complement(30414..31043)
                     /locus_tag="ONT64_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR2302725.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            31213..32505
                     /locus_tag="ONT64_001300"
     CDS             31213..32505
                     /locus_tag="ONT64_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2302726.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(32581..33243)
                     /locus_tag="ONT64_001301"
     CDS             complement(32581..33243)
                     /locus_tag="ONT64_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR2302727.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            33468..33752
                     /gene="groES"
                     /locus_tag="ONT64_001302"
     CDS             33468..33752
                     /gene="groES"
                     /locus_tag="ONT64_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR2302728.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            33803..35428
                     /gene="groL"
                     /locus_tag="ONT64_001303"
     CDS             33803..35428
                     /gene="groL"
                     /locus_tag="ONT64_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR2302729.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(35517..36665)
                     /locus_tag="ONT64_001304"
     CDS             complement(35517..36665)
                     /locus_tag="ONT64_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2302730.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(36767..37351)
                     /locus_tag="ONT64_001305"
     CDS             complement(36767..37351)
                     /locus_tag="ONT64_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCR2302731.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(37478..37909)
                     /locus_tag="ONT64_001306"
     CDS             complement(37478..37909)
                     /locus_tag="ONT64_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCR2302732.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(37927..38247)
                     /locus_tag="ONT64_001307"
     CDS             complement(37927..38247)
                     /locus_tag="ONT64_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2302733.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            38547..38687
                     /locus_tag="ONT64_001308"
     CDS             38547..38687
                     /locus_tag="ONT64_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302734.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            38985..39242
                     /locus_tag="ONT64_001309"
     CDS             38985..39242
                     /locus_tag="ONT64_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HCR2302735.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(39213..39521)
                     /locus_tag="ONT64_001310"
     CDS             complement(39213..39521)
                     /locus_tag="ONT64_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302736.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            39599..40345
                     /locus_tag="ONT64_001311"
     CDS             39599..40345
                     /locus_tag="ONT64_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HCR2302737.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            40360..40563
                     /locus_tag="ONT64_001312"
     CDS             40360..40563
                     /locus_tag="ONT64_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2302738.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            40668..40967
                     /locus_tag="ONT64_001313"
     CDS             40668..40967
                     /locus_tag="ONT64_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302739.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            40967..41149
                     /locus_tag="ONT64_001314"
     CDS             40967..41149
                     /locus_tag="ONT64_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302740.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(41155..41631)
                     /locus_tag="ONT64_001315"
     CDS             complement(41155..41631)
                     /locus_tag="ONT64_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302741.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            41684..42409
                     /locus_tag="ONT64_001316"
     CDS             41684..42409
                     /locus_tag="ONT64_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HCR2302742.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            42583..42924
                     /locus_tag="ONT64_001317"
     CDS             42583..42924
                     /locus_tag="ONT64_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302743.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            42917..>43206
                     /locus_tag="ONT64_001318"
     CDS             42917..>43206
                     /locus_tag="ONT64_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCR2302744.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT        15055 a         6467 c         8492 g        13192 t
ORIGIN      
        1 caaataacaa caaagtggct ggcacaaaaa aaataaataa aataaattct attactggtc
       61 aatcattaaa tttttcatat aacaaaagaa aggtattaag caatataagt ttttcaactg
      121 aaatgaataa actattcatg atatcaggag aaaatggaac agggaaaaca acactgatga
      181 aattatttac taaacagttg ccggttagta ataatcaatt attcgtcaac gggatagata
      241 ttaatatgat tgataacgaa tcattaagaa ggttaatttc aatcgcaaca caaataccat
      301 atataattaa tagctccgtg aaggagaata ttttaatggg gactaaaaac tatgataaaa
      361 aaaaattcag taaattaata gaactattgg gaataagtaa atttatgacg aaattaggta
      421 ttactttgga cgacaatatt tatgatgaaa aaatattatt atctggagga gaaaaacaaa
      481 aaataagttt agctagggcg cttattaaat cagcttcagt tattatatta gatgaaccat
      541 ttagaaattt agataatgaa actcgcgata atttgaggga ttatatacta aatacaaaaa
      601 aagaagtttg ttatataatt ataactcatg atcacattct agataatttt ttggattcaa
      661 atgatgaaat attatattta aataaggagt atgataataa tgaatagcaa aaaacttcag
      721 atgggtgaaa ttgttagagg tcctagtcaa atatctattg atgttacata taaatgtaat
      781 tttagatgta aacattgtta caatgcgagt ggtggagaca attgttttat ggataatgag
      841 ttgactgatg atgaactgtt agaacttaca aaagatatat gtaatttaaa gccatataat
      901 atttgtttct gtggaggaga aactttactg agaaaaaaaa tagttttatc ttgtgccact
      961 ttattaaaag aggcaggtat tcctaatatt gctattgtta ctaatggatc attagtaact
     1021 caggatgttg caaaagaatt attcaaagca ggtattaatc gatttcaagt aagtttagat
     1081 ggaaaaaatg ctagtagcca tgaagcactt agattatatc caaacagttt tgaaaaggca
     1141 attaatgcta tcaaattatt tagagaattg tccgaagaag tggatgttgc tttttgtcct
     1201 actagtttta attacaatca attacgagaa gtatatcaac tagtaaatac tcttaaagtg
     1261 aagtctttaa gaattcaacc atttatgctt agtggtaggg gaatgcaaaa tagggaaata
     1321 gtgccaactg tttctcaata tagaaaacta gtttctgata ttaactatat taaaagaaat
     1381 gaagattata ccaagataga ttggggagat ccaattgatc atatcatccg acacagaact
     1441 ttattgaaag atttttcgaa ttttgtttct attaggtcag acggaggatt aatggcatca
     1501 ccttacttgc caattcaagt tggaaatatt aaatgtcaca gtctttctaa atattggcga
     1561 gcaggacttt ataaagtttt ttctttagat ttagttcaag atatagctac tcaagtttct
     1621 tcaatacaag atatgagtaa agaattaaaa aaaggacata ttaaatggct taatgaagat
     1681 atagaaattg atattctcga tgaatacaaa catgatattt taatttaggt ttatcattgg
     1741 aaagtttaga taaacaaagt acttttagaa aggagggaaa aaaatgattt ctgaacctca
     1801 aatgaataat atgtatgaaa gcccaactat aatgggagct ggtgaagctg cgccaagagc
     1861 ttgttcagca attatggtgt gtgtgttatt agcatctgca ggagtaggta tccaagcagg
     1921 agcggtgtat acttatgctg tagttgctac agtagctgta gctgcagccg cagtttggaa
     1981 aacagtagga ttagttggtt aggaattaat aggagactaa ctgtctccta ttgattccaa
     2041 tgattaagct aaggaggaat tttaagaaaa taatgaaata caataattta aaaccgaaat
     2101 tacttaatga gaacatagaa tatataagag aagaaaatta tggatattta accatagttc
     2161 ccaaaaatgg aactgaattg aaagataacg ttttcaatcc aacagtaaga tatatattgg
     2221 ctaactgtac tggaaaaaac agtgtaaaaa aaatagcgaa tggtttaaaa aagaagtttc
     2281 ctagtttaga atatgaaaaa ttctatcaag atgttgtagg aactattaaa tttttcaaac
     2341 actataatat tattgatata ggagattata gtatggaaga aaagaaaata tataaaattg
     2401 aacaaaattt agaattagaa ttactaaatg aatcacatta tgaagaaatt tataatttta
     2461 tcacagatca tcctgaaagt atgtattttc atgaagatat ttatcatcaa ggaaatagta
     2521 aagaattaga agttgtcctt agatataatt tattttcgtt tacgagtgaa ttttactttt
     2581 taagacataa agagagaatt gtaggaatgg tttctataat tgttaatcca tttcaaaact
     2641 tatcctacag tatagtaaat tacctcatat gtgagcctgc atatactacg actattttga
     2701 caacagtatt agaaaatatt aacgaagttt caattttaga agtaaagaag ctagtgattc
     2761 atttgctaaa ttcaacagaa gatgaatcac taatccccta tttaaaagat tcacattttt
     2821 ttaaagaagc ggtactatct aaagaatata gagagcatga tgttttgaga tacgcttaca
     2881 tttattaaat aaaatttctg atagtatttt aataattcgg atcatatcat aagatatagg
     2941 agggaaaaga agtgatttta ttgattttgt ctctcgtatt actagttgta tctaatctaa
     3001 tgaaaaataa tcctcctaca aaaattaatt ctttttatgg ctttagaact aaaaagtcaa
     3061 tgaaaaatga aaaaagttgg catttagcac aaaaattgtt gggggatttt tcaaaaaaaa
     3121 aagttatccc agtatttttg atgggtattg tttttttcag tatagagatt tatagtttag
     3181 ctattttaaa aaatgaaaat tttttcgtta gtgtattaat gggtgaaagt ttaatcctaa
     3241 tactgatttt gtgtttaatt gttcatgaag tgaataaaca attgtgaatt aaaaaaataa
     3301 aaacctagta taatcacttt aataaattgt tggtagaagg tgatcaaaat gggagtaaac
     3361 gaaatgatga aagtcagatt gagtttttaa atgatacctt cctcagaact tcaaaacgaa
     3421 ttcaaagatt cgttgacaaa tcttgagcaa gatcatttaa aaaaatattt tctcttcatt
     3481 tgacgaaaaa tgtttagaaa tcttatataa gtgaagataa agtaattggt gtaattaatt
     3541 gtagagaata gcctttgagc aattgaagcg atacaattat ttagaaaatg aacttcctcc
     3601 agcatactac atgattcctt acgggacgat gttaaagaag aaagcaagaa attttagaag
     3661 tattagtaga taaactaatg ggcttttctc aatttaaggg agtagcccaa atgtttatca
     3721 aatatggtgc agatacagta gcaaccatgt ttacagaaga atatcaagta gactattatg
     3781 aagtaataga taaatgtgta cattttgttt atgaaaaagt gtggaatttt atgaattttg
     3841 cgataagtga agaaatctta cgagtacaag aaaacagaac aggaatattt accagagaca
     3901 gaaagcttaa tttgattgcg cattatatac ggaatcgctg gtcatcaata ccaaatttta
     3961 attataaaaa ataattatga acaatctttt atacaatata cacaataaat caagtagtat
     4021 ttatgaaaaa agacttcaaa aacagaatga ttttctatca gtcttagaaa ttatagagct
     4081 aaaaattttg gtcacgcgta gaaacgcgtt ttttttgttt ttttgatagt aaagaacgta
     4141 caaaacaatg agaatttaaa agattgtact accagttttt ttaccttttc atttgagttt
     4201 agagtaagta aaatattact ataaaaacac atattcttga agaaatcttt tttttgctgt
     4261 attcatttag attatgatag agaaaatacg tgagaggggg acctgggatg tggctatctg
     4321 atgaaaaaat tcagtggcgt agctatcaga agcgtatatt gactaacttt gctacttatt
     4381 taaataacaa ttcagtccat ctagtggctc caccaggggc tggcaagacc tttttaggaa
     4441 ttgagatgat ccgacgtttg gattgtaaaa ccttaatttt ggttccttcc ctaatattga
     4501 agaaacagtg gttaacaaca atcgaggaat atttcttagt aaatccagca gatagttgct
     4561 atctttctgg ggcgattgaa catcctagtc atattacagt ggaaacgtat cagaccattt
     4621 acaaccgact gaaggaggag ccaacttatg gtgatcagct ggagctgtta gttttttgat
     4681 gaagctcatc atttaaaaaa aagctggggt gaattgttga ttgacttgaa gaaaaaacaa
     4741 ccgaatctgt tgacgatttc cttgacagcc acgcctccga ttgatagctc tcagacggag
     4801 tggaagcaat atattgagtt aaaatggcct gattgacgaa gaaattagca ttcccgagtt
     4861 ggtaaaggag gaggttctat gtcctcatca ggattttcta tatttgattc cggctaatca
     4921 ggaaactact cagcattatt ttaccttttt agagaaacaa aatgagctgg tggaagcgtt
     4981 aatggaaagc caagagacgg tggattattt gctttcttta gaatttgttc agcatccgct
     5041 tgagcattcg aaggaaattt atgaaaattt tgagctgttc actagtgcat taatttactt
     5101 gaatcatcat aactatgaat tgatacccga acattggcag ctattaggca gttctaagca
     5161 gcaaggtaaa aaagcttcag ttccatcttt aggtaaaaaa gagctgatca ctatttatca
     5221 gcagatgttt gaagatcaac cggaattgcc tattttttat caattgaaaa aagctggatg
     5281 attgtttgat gagtcgctgc atttgtttcc agagtatcca gacactgttg gtagtcaggc
     5341 gattcctgag aaaaagcaag cagtttgtca aattttgtta aaagaagaag ttcagctgaa
     5401 gcaagaactg acagcggtca ttttgatgga tcgtatttgc aaggaggtat tagcaggcag
     5461 tgatggtacc ttagcttatg gcgtggttcc tttgtttgat gaagttgttc gtctattagg
     5521 taacactcag caaatcggtg ccatctgtgg agagttctta ctgttaccga aaatgctagt
     5581 aactgttgag ctggcagaac tagcgacggg tatgaatgaa catccttata tgtcgggtta
     5641 tttttatttg acggtggatg acaaaaaccg ccaagcattg atgcaagcag ttactcgcct
     5701 attaaccgaa aagaagttga atattttggt aggaaccgtt agtttgctag gagaaggctg
     5761 ggattgtcct gcaattaatc tgttgatctt ggctaatcaa gcttccactt ttgtgcaaac
     5821 acaacaacta cgagggcggg gcttacgcct aggtgcacaa aataaagtaa cgaacatttg
     5881 gcatttagcg attggattac cgggagttcc tctgtctgaa cagcctgatt tgaaaagaat
     5941 gctacggcgt ttgggcaata tttcaggtct gacctttgaa tcttcaccaa aaattgagag
     6001 tggtggcgat cgctttgctt taccactgga atatacgacc accacgatgg aaacttatac
     6061 tcagtcgatg ttctattttt cttgtgaacg aagtcggttg aaagaacaat ggagtactgc
     6121 tttagctgat ggctggcacc tatctatgcc aatcgtcgtt aggaaagtcg atcaggtaaa
     6181 gcaaaaacaa gaaacttcat tgccgatagt agtaaaaaat atagagcctc taagcttctg
     6241 gtcagctata tttaaaggta acttgctgga atatttccaa attcgtaggc gacaaaagct
     6301 atgggaaaag gaatgtgccc gcaaaaaaag cttggccata tgcgttcaaa cgacgttgca
     6361 gcgccttggt gaagttagtg atagaaaagt aatcgtagcg gctgatcagc aggaatttag
     6421 atgctatctt gagaatgctg gctatcgaga aggacagctt tttactgaag ctatgaggga
     6481 gcttttagca ccgctggaga atccgcggta tttaatcgaa gggaaaaaga tagttctgac
     6541 ggtaccagaa cgctttggcc gaaataagca aatcgctaac ctttttctaa acgaattaaa
     6601 tcaatcgtcg ctgcccaaat gtcagttggt gtatactaga aatcctgaag gacgccaaaa
     6661 gctagtgact tatcgaatga ggcaagtctt ccagacccgc gatgaagtaa ttattcaaaa
     6721 aaacatttgg acgtaagttg atgaaaataa attttgctag agtagaacaa gtccgagaat
     6781 taagttgcta cttaactgat aatgccaagg acaggagaat cttttcttga cgactgaaag
     6841 aaactatttg acttatataa cggtgtgtga tgaatatttg caaggcattc tcttaaaaag
     6901 tactcgtaat agtttttttg aaaacaaaac agtactttat taataggact gacagagcga
     6961 tataagaaca tctgcagaca gaggtgaatg cttttctcat gatcgatgaa acaatggtac
     7021 ttcggatatt cttgctttta gtttgaaccg ttgtgatttt ctttgattcc taaaagtagt
     7081 tttatagaat gaaaatcaag catacggaat gctcctttga ttgtgttgtg gtgattcaat
     7141 ctaatggagc ttctatatgt ttttttattt gcaaaaagaa ccgatagaat atttttatca
     7201 gtcttagaaa ttatagagcc aagaaatttc aagcacacat aaaaacgcgt gttttatgca
     7261 ttttatgagc aaaaataact aaagaccatt acgtaaaaca taggtaaatc agcattcaat
     7321 ctcaaattat gtgatcaaat gttgatatta tttgattata agttttcttt tgctattatc
     7381 agtctgcaaa taaacagatt atagagtatg aatgtagata gttcaatctt tgtttacgac
     7441 ctcacatgtt tttttactca agagaaagta atataaagtg actatatttg acagaacttt
     7501 acataaaaaa agtagttagt tttctgtagt aatcagaaat gtaaataaat taaaaaaaag
     7561 aaatttagca gttatatatt tttaaaatat tttgggtgag tagtcaatac aacattttgt
     7621 tcatcaagca ttaaatgaag atgcaaggat gttaagaagt tagcttcttc agggtcttgc
     7681 atggtcaagg ctttaaatag ttgatgatgt tgttccataa ttttttccca ttttaatcct
     7741 tccgttttta ctcgtaacca acgaaatcgt tccaagtgcg tattattatc ttccaaccat
     7801 tgccatactt cttcccgttc tgcaatttca aaacaagctt tatgaaataa attatcatta
     7861 tgaaaaaaac tacgagcatt cttttgacta acagcttttt cttgttctgc aattatagtt
     7921 tctaaaactt gtttcttttg attattcatc ttggcacaac actctagcat aatttgtcgt
     7981 tctaaatgtt ctctgacaaa acgagcattt tttgctaaat ctaagtcgat taatgagata
     8041 taatttccac tttgaggaat tgtatcaaca agtccttgat gacggagttg aatcaatgct
     8101 tcacgtattg gagtacgacc gatagaaagc gtttcttcca attttttttc tgatactttt
     8161 tttccaggtt ctaagtcagc gtagataatt tgctgacgaa tagattgata agcttgattt
     8221 tggagactag ttttaattgt catatttaat ccctctcaaa atttcatttt tgaatttgat
     8281 attttagttc tatgctcgtt atataatatc aaatttcaaa gtaacatact agctattaaa
     8341 aaaacaaaaa atactcaaat aagatcaaag agaaattaag ctttaattta agttagttct
     8401 aaaaaatata tattggtgtg cttttactaa aaaaagacac aataattctg acatggtaga
     8461 aaaaagaata tttttagttt tttcttattg acagcgatta cattcgattt tatactacaa
     8521 ttgtaacgga aactaacata tcagtatgga ggggtaaata tggttttgat taaagactgg
     8581 caagattcta tcgaaggatt gaacaaaatg caagttcaac taccgtattt taatgtcgaa
     8641 gcggtcaaac aagaaggtca gaaacatcca gtgtggcttc attttggtgg aggaaatctt
     8701 tatcgaggat ttcatgcaga aattgctcaa tgcttagcag atcaaaatga attagaatcc
     8761 ggagtagttg tgtgtgagac atttgatgaa gaagtagtag aaaaagctta tcatgactac
     8821 aacaatgata ttttggagat tgttatgggt gaagatggtg aactcaaaaa aagactttta
     8881 gcagtaacag ctgatagttt atattgcaat ccaaagaata taacggacta tcaaagagtg
     8941 gttaaatatt ttgaagatcc tacattacag tttacaacgt ttactattac tgaaaaagga
     9001 tacagtttaa aagattctaa aggacaattt ttttcggtag tggaagcaga tataaaatct
     9061 ggacctacca atcctcaaca tacgatgagt attgtgacat ccttgttgta tagcaggttt
     9121 aatgctggag agttaccgat tgcgatggtt tctactgata atttctcaca gaatggtcaa
     9181 cgttttcaag atagcatttt tatgattgca gaagcttggc aaaaaaaagg atacgtttct
     9241 gaaaaattta ttgcttattt aaaggataaa gaaaaagtga cttttccttg gagtatgatt
     9301 gatagaatta cgccaaatcc ttcggaaagt gtcaaaatgg atttattgaa ggcgggtttc
     9361 gaagacttag atataattca tactgcgaaa aataccaata tcgctgcttt taccaatact
     9421 gaaaaagtcc attatctagt cattgaagat agttttccaa atggtcgacc taaactggaa
     9481 gcggctggtg tgattatgac agatagagaa acagttgata aagcagatat catgaaggtt
     9541 acgacatgtt taaatccgct ccatacggca ttagcaataa caggttgttt gttggggtat
     9601 aaatcaattc aggcagaaat gcaagatgat gatttggttg gcttaattaa agggataggt
     9661 tatgtagaag gcttaccagt agttgaaagt ccaggcgtta ttgatccaaa acaatttatt
     9721 gatgaagtaa tcaataaacg tttgcctaat ccctatattc cagatacacc gcaacgaatt
     9781 gcaacagata cttctcaaaa agtagcgatt cgctatggtg aaacaattaa aaagtatgtc
     9841 gaaaaagacg gaaatgcaga aaatctaaca tttattccta tcgctattgc tgcttggtta
     9901 cgttatttat tggcagtcga tgatcaagga aaaaattttg tgccaagtcc tgatccttta
     9961 ttggtagagt tgcaggaaaa attctcagta ttgaaattag gtgaacaaga tcctgcaaag
    10021 attcattgtg cggtacagga aatcttagaa aataaggaaa tatttggttt agatttatat
    10081 caagttgaga taggaaagaa aattgaaaaa atcttttgcg aaatgctagt aggtcctcaa
    10141 gcagttcgta aaacaatcaa taaatatata acagaagttg gaggaaagta actatggaaa
    10201 tgacttttag atggtatggg gcaaatgaag aaaaaatctc actacaagaa attcgtcaga
    10261 tcccagcaat gaaaggaatt gttggaacat taatggatat accagccgga gaggtctggc
    10321 cgagagaacg aattaaagct ttaaaagatg aagtcgaagc caatggcctt accttgaaag
    10381 ttattgagag tgttaatgtt catgatgata tcaaaattgg tttgccaaca cgagatcaat
    10441 acatcgagaa ttataaacag acgattcgca atttggcaga atttgggatt gaggttatct
    10501 gttataattt tatgccagtc tttgattggg taaaatcaga tttggattat catttagatg
    10561 atgggtcttc tacgttagcc tatatttcaa aaaatattcc tactgatcca gaggaaatta
    10621 ttagaaaagt agaaaattct actaataatt ttgaattgcc gggttgggaa cctcatcggt
    10681 tatcacaaat caagcaatta ttagaagcct ataaagaagt tgatgaagcg aaattgcgag
    10741 agaactttgc atattttctc aaagcaatta tcccgacatg tgaagaagtt gggattaaaa
    10801 tggcagttca tccagatgat ccaccatatt ctattttcgg attacctcgt attgttaaaa
    10861 atcgtgacga tttagaatgg ttgtgtaatg tagtggattc tccttctaat ggtattacgt
    10921 tatgtactgg aagtattgca gaacgaccag ataatgatgt ctatgaaatt ctatctgaat
    10981 tttgtcaacg ggatcggatt ccttttgcac atgttcgtaa tatcaaatat ttaagtgata
    11041 atggactcga tttttatgaa gcgccgcata aatcagaata tggttctcta gatatgtata
    11101 agatattaaa aacaatgtac gataatggat ttaatggcta tattcgtcca gatcatggtc
    11161 ggatgatttg gggagaaacc ggtagacctg gttatgggtt atatgaccga gctctaggtg
    11221 cagtttattt aaatggtatt tgggaagcgc ttgaaaaggg ggcgagtaaa taattatata
    11281 atggagtcta tttagaagaa aaattttctc ttctaaaaac taagggaagg aaaggagtgc
    11341 ttaaatatgt ttttagataa gaacttttta ttagagaatg aaatagctaa aagacttttt
    11401 cataaatttg cagaacacca accaatcatc gattatcatt gtcatttaga cccagcatgt
    11461 ttgctagaaa ataagcaata caagaatttg actcaggtat ggttaaatga taaaggagct
    11521 ggagatcatt ataaatggcg attgatgcga gctaatggga tagaagaaga gttgatcagt
    11581 ggtgcaggta atgattatga aaaattttta gcttttgtaa aaacagtgga gaaggcgcca
    11641 ggtaatccta tttttgaatg gtctcatttg gaattacaac gttactttga tattgatttg
    11701 acaatttctg agaagaatgc accagaaatt tggcgacgtg ccaacgaaaa aatccaaacg
    11761 gattcctatc gcccaaaaga attgctgaag atgatggatg tgaaagccat ttgtacaacg
    11821 gatgatccta cagatgactt gcgatatcat actgagcttt ctaaacagga taattctttc
    11881 aaggtattac ctactttccg tccagataag gtctggagta tcaaatcatc aggattttcg
    11941 aactatattt taaaacttag tcaagcagcg ggtataccaa tcaaaaactt taatgggttg
    12001 aagcaggcat tgagccaacg agtggattat tttcatcaaa ttggctgtcg attagcagat
    12061 cacggtttga atacttattt ttatgaaata gcagatgaaa aaatactcga caatctgtta
    12121 ataaaagctt tagcaggaaa aacggatttc catttatcag aaattgcaca atttgttaca
    12181 ggtattcaat tacatttgat gtctgagtat acaagatatg gttggacgat gcaaatgcat
    12241 ttaaatgctt taagaaataa ttcaagtata ttaaaggaac aaattggagt agatgtcggt
    12301 ggtgattcga tgggggatca agctagtttg acacaaaatg ttgtagcatt gttcgacact
    12361 gctgaaaaga gtgggtgctt accaaaaaca attttatatt cattaaatcc aaccgattgg
    12421 cttccactag caacagcaat gcaaagcttc caagggggta tgattcaaaa aatccaattg
    12481 ggatgtgctt ggtggttcaa tgatacagct gaaggaatgc gtcaacagtt gactacgatg
    12541 gcgcaacaaa gtttgttgtc gaattttaca ggtatgttga ctgattctcg aagttttcta
    12601 agctatcctc gccatgagta tttccgtcga gtattatgtc aattactaag cgagtgggtt
    12661 aaagcaggtc gaattccaga tgatgaaatg tatcttagtc agatagtgga gaatatttgt
    12721 tatcgtaatg cgtataatta ttttaatttt tttgaaatcg aaagtagaaa ataatacaaa
    12781 aaataaaagc ttaaatttat ttattgtaag ttagtacaac tagctagagt atgtatccat
    12841 aaaagaagga gatagatatg aaaaaagtag aaattttaag taagttagaa gcggctggag
    12901 tcattgcggt tgtacgtgga aaaaataaag atgaggccat taaagcaata aatgcaatta
    12961 ctgcgggtgg aattaaaggg attgaactaa cttttacagt tccgaatgct gaagaaataa
    13021 tcaaagaaat tgctaaaaaa tatgctgatc aaaaagatgt aataattgga gccggtacag
    13081 ttttagatgc gtttactgct cgtattgcaa ttatggctgg agcagaatat atcgtaagtc
    13141 catcttttga tcgacaaaca gcagagattt gtaatttata tcaaattcct tatttaccag
    13201 gttgtatgac aattactgag ataaaggagg cacttaccgc tggagtagaa atcattaaac
    13261 tatttccagg ttctgctttt ggtccatctg ttgtaaaaac gtatcaagct ccattaccat
    13321 atgtaaatat aatgcctact ggcggagtaa gtctagataa cttagaagag tggtttgaag
    13381 caggcgttgt tgcagttggt gtaggtggga atttgttggc tcctgttgaa aaaggtgatt
    13441 ttgaagaagt aactcgaata gctagtaaat atatagataa gttttatgag ataaagaatt
    13501 attaggagaa aaaataaata atactataaa atgtaaaaaa taaatactaa ctctttatac
    13561 ttcagagtat agtacgtaag ttggcagttc aacagaatga aaacgcttaa caataggaag
    13621 gagcgcaaat tatatggaaa gaaaatttgg tctaagagac aaaataggat atatgtttgg
    13681 agattttggg aatgatttca ctttcatttt tgctagttct tttttaatga ttttttatac
    13741 aaaagtattg gggataagcg gagctaccgt aggaacatta tttttagtag caagatttat
    13801 tgatgcagtt acagacataa caatggggcg tattgtggat ggatcaaaac catctaaaac
    13861 tggcaaattt aaaccgtgga ttttacggat gagtggtcca gtcgctttag caagtttttt
    13921 aatgtatcaa acatcaatgg catcagcttc tatgagtatt aaagttatat atatgtatgt
    13981 aacatatata ctatggggaa gtatttttta tacatctatt aatattcctt acggttcaat
    14041 ggcatcagct atcacttctg ttgcagaaga aaggacagcg ttatcaacat ttcgtagtat
    14101 aggagctacc ttagcaggta tgttcattgg cgtgttaaca ccaattttta tttatacaag
    14161 agatagttct ggagctcaaa ttgtacgtgg tggatcaact tttacaattg tggcaggagt
    14221 attttcagtg tgtgcagtta tttgttacgt aatatgttat aaattaactg tagaacgggt
    14281 taaaagtgat gaatcgcagt taaaagtttc attgttgaaa tctttaaaag gattagctaa
    14341 aaatagagcg ttgctttcta taatagctgc agctattttt ttgttgcttg ctcagctttt
    14401 aacaatgagt atgaacaatt atgtatttcc agattattat aataatgcac agggaatagt
    14461 tttaatgaac tttatcaatc ctattttgat gttggtactt gtttcacctg taagtttaat
    14521 tttcagtaaa aaatacggta aaaaagaatt ggcgtcagta gggatgttct tctctgctat
    14581 tgtatattta cttttatttt tgataaagcc tagtaatatg tatgtattct tagttcttac
    14641 ttctgttgga tatatggggt taggtatttt taatacagtc atttgggcga atattactga
    14701 tgtaatcgat gatcaagaag tgaaatctgg ccaaagagaa gatggaacta tttatgcagt
    14761 atattctttt gcccgaaaaa ttggacaagc tttagctggc ggtgtcggag gttggactct
    14821 ttctataata gggtatgatt cgttagcaaa agtacaaagt ggagaagttt tacaaaagct
    14881 gtataactct tcaacgttga ttccagcagt ttgtttcttt gttgtaggat tgattttatt
    14941 tttcgtatat ccattatcta aagaaaaagt attagaaaat tataatttgc taaatcaaaa
    15001 acataactag gagaaaaaac atgaacagtt ggatattttg tattgattca gatggttgtg
    15061 tcatggatac aatgacttat aaacacaatc tctttttcgg gccaattgct gcagagattt
    15121 ttgatgttca tcatagggaa aaattcctac aggattggaa taatattaat ttatattcag
    15181 agactcgtgg aataaacaga tttttaggtt tagttaaaac attagaaaaa aatgatgtac
    15241 catcgattaa tcgattaaag cactgggtat ctgtaacaaa agaattgtct aatccatctt
    15301 tgaagaggga aatagaatac tctcagtcag aggatttatt aaaagcttta aaatggtcgg
    15361 aagaagtcaa tcgacagatt agtcaagcta aaggattaga taaacccttt ttaagtgcta
    15421 gagatactat aaaagctctg aaaaaatatg ggaagataat tattgtaagc tcagctaata
    15481 aggaagctat tcaagaagaa tgggaaagac atgaattatt atccttagtt gatgaactgt
    15541 gttgtcaaga taaaggaaaa aaagaagaca ttattcgaag tgtaattgaa aatggttgtg
    15601 atttggataa gatattaatg attggtgatt ctcctggtga tctagaagct gcaaataaga
    15661 ataaagtttt cttttatcct attttggtta ataaagaaaa agaatcatgg gaaaacttaa
    15721 gaactgatat tttatttaaa tgtttatcgg gagagtatat agatattgaa caaaaatata
    15781 tagatacttt ttggaaaaat cttactaata agtagaggag aaagaaaatg actataaaag
    15841 tagacttacg aaaaaaacca tatcatttag atgaagatgc tatttcttgg attgaagaga
    15901 cgattgatgc tatgacttta gaagaaaaaa taggtcaatt atttgttaat atgggatcta
    15961 gtcgtgataa aaattactta agtagtatgg tcaatcagta ccatattgga gctgtacgat
    16021 ataatccagg aactgctgat gaggtatatg atcaaaacta tatcctacaa acagaaagca
    16081 aaattccttt attaattgct gctaatacag aagcaggtgg taacggagct tgtacagatg
    16141 gtaccgaagt tggtgtagaa gtaaaaattg gtgcaacaaa tgatgcgaaa tatgcttatg
    16201 aattagggag agtttctgga attgaagcat cggctattgg atgtaactgg agttttgctc
    16261 ctattgtaga tattaataga aattggagaa atcctatcat ttctagtcga tcttttggac
    16321 aaagccctga taaagtttta gaacttagtt tagcttatat gaaaggtatt caagagagtg
    16381 gaattttgcc tgcagctaaa catttccctg gtgacggtat tgacgaacga gatcaacatc
    16441 tatcttttag tattaatagt ttaagtacag aagaatggga taattctttt ggaaaagtat
    16501 actcaggctt aatagaagct ggattacctt caattatggc aggtcatatc catatgccag
    16561 cttattcaaa acattttaat cttgatctga aagatgaaga tttacttcct gcaactttat
    16621 caaaagaatt aatcactgac ttacttagag gaaaactcgg gtttaatgga ttagtagtta
    16681 ctgatgctag tcatatgtta ggaatgactg gggcaatgaa acggtcagaa ttattgccca
    16741 cttctattgc ggctggatgt gatctattcc tattttttaa tgatccagat gaagacttta
    16801 attatatgat ggaaggttat tacaaaggaa ttatttcaga agagcgttta cgtgatgcat
    16861 taagaagaat tttaggctta aaggctaaac taggtttgca taaaaaagaa aagaataaaa
    16921 ttttgttacc gaaacaagaa gctatggaaa aaattggttt aattgaaaat aaatcaattt
    16981 ttaaagaggt agctgatcaa gcaattacac tagttaaaga taaacaagat atttggccta
    17041 ttgtaccaga aaaatataaa aggattttac tggtagatgt acatggggta aaaggtgggt
    17101 ttggtgaagt agttgctgga aatagacctc aggccattga tttattgaaa cagattttgt
    17161 cagcaaaagg atttgaagtt gaaaaataca tttcacctga agaagaattg aaaggattat
    17221 cgcaagaaga aaaaattaaa aatatagctg gaatatatgc tgctaagaga ccaatcaaag
    17281 atttaactga aaaatatgac ttgattatta atatagctaa tgttaaccca ggtacagttc
    17341 aaagaattgt ttggccagca agtaaaggta ctccggatat tccattttat attcatgaaa
    17401 ttccaacaat ttttgtatct gtacagtgtc cgtttcattt agctgatgtg cctcaagtaa
    17461 aaacttatat caatacttat gatggaaaac aagaaacttt agaaatatta gtggacaaac
    17521 taatgggcct ttctgaattt aagggaataa gtccaataga tgcttattgt gggttaaagg
    17581 atacatttat ttaatattta aattatatag gagtgattta catgttatat cccattatga
    17641 cagagacgag gcaagtaatt gatttaagcg gtctttggag atttaagtta gatacaaatg
    17701 atgacttaca agaagaactt gctttagaac caatggttga aaaagagagt tatccaattt
    17761 cagttccttc ttcttataat gatttatttg aaacagagaa cattagaaat catgttggat
    17821 gggtgtggta tgaaaaagaa gtgattcttc ctaaaaaatt attagaagaa agattagttc
    17881 ttcgttttgg atctgcaaca catgaagcaa aagtattttt gaatggaaaa ttgattaatc
    17941 aacacaaagg agggtttacg ccatttgaat cagaaattaa tcagtttata aaagatggaa
    18001 gtaacagaat tacagtggct gtaaacaatg ttattgacga tagcacttta cctgtaggga
    18061 ttttgaaaga aacgatacag gaagatggag acataaaaaa agaaaatttg gttaattttg
    18121 actttttcaa ttatgcaggc attaatagac ccgttaaatt gtatacaact ccgaaagact
    18181 atataaagga tgtcactatt aatacaaaaa taattgattc agatgcaaat gtagaattta
    18241 atatcgatac agaatggacc ggagaaattc taatagcaat ctatgacgaa gaagctcaac
    18301 ttatagcaga agctaagggg aaagaaggag tgattcaact taagaaccca acactttggg
    18361 agcctctaaa tgcttactta tatagtatga aagttagttt aattaacgaa aataaagtga
    18421 ttgatacgta cacagaagaa tttggcattc gtagtattca ggtttcgaat gggaagtttt
    18481 tgattaataa taaacctttt tactttaagg gatttgggaa gcatgaggac acgtatatta
    18541 atggtcgtgg tttgaatgaa gcagtaaata tcaaagattt taatttaatg aaatggatcg
    18601 gtgctaattc atttaggact tcacattatc cttattcaga agaaattatg cgcttagcag
    18661 atcgagaagg tattgtagtt attgatgaag tacctgcagt aggattacat ttgaatttca
    18721 tggctacttt attagatgac aacgtagaaa aacatgatac ttggaaagaa atacggacag
    18781 aaaatgctca caaaatggcg attaaggaat tgatagaacg cgacaagaac catccgagtg
    18841 tcgtaatgtg gtctattgca aatgaaccag attcggattc tgaaggcgct aaagaatatt
    18901 ttgaaccttt agttgaattg actaaagaac ttgaccctca gaagagacct gtaacaatcg
    18961 ttacttactt aaagtcaaca ccagatattt gtaaagtagg agatattgtc gatgttttat
    19021 gtttaaatcg ctattatggt tggtatgttt caggtggaga cctaaaacat gcaaaatatc
    19081 tgttgaaaaa agagctagat ggatggatgg aaagatgtcc cgatacgcca ataatgttta
    19141 cagaatatgg agcagataca gtagctggac ttcatgatac agtaccaaca atgtttacag
    19201 aagagtatca agtgggttat tacgaagcaa atcacgaagt aatagataaa tgtgacaatt
    19261 ttgttggtga acaagtgtgg aattttgcag attttgaaac aagtcaaggg attttaagag
    19321 tgcaaggaaa taagaaagga atctttacta gagatagaaa gcctaaaatg attgctcact
    19381 atctaaagaa acgatggtca tcaataccaa atttcagtta taaaaaataa tttacctttt
    19441 ataaggaata taaaaaagga gtggctgctt aattgccaac tccttttttt tataaaaaga
    19501 ttttctgttt aaagaaggat atttttttct agttttttcg aaaaaaaggc gctttctgat
    19561 tgttgtatta gtggaaaggg gaaaaaagga gcatatttat catgcaacaa aattacaatg
    19621 agaaatctat acaacatcaa tttgatgcct actgtaaaaa agtattacga aacgaaacta
    19681 agtctattcg aaaacgaaac attaacataa cagataatga ggaaccattg acgaattcaa
    19741 atgaaggtaa gtactcccaa actcattttg atgaacaaga tgtctatttc ttatttggaa
    19801 tggaaatatt aatttcagat caaaagttat cggcagccat tgatcattta tcggaaataa
    19861 gaagaaaggt tatcctactt tattattttg caggttttaa tgatactgaa atcggtaaaa
    19921 tcctcaatat gagtacaagt ggtatctggt atcaacgaaa gaaggcagtt gaacaattaa
    19981 aaatggggta tgacttatgg tagacaaccc caaaaaacgg cagtatccat tagttcctct
    20041 atccattatc caatctgctc tagaagaaga tccaatttca atcaagatga tactcaaaca
    20101 ttatcatagt tatatagcta agttatgttt gacaaatgga tttaatgagg cggggcaatt
    20161 tattacctat gtagatgagt atatgctaag acagctagaa attaaattga tagaagcgat
    20221 cttaaaattt aaaattaact aactaaaaag tcccctttcc acttattctg tttaaaagaa
    20281 tcttctaata gttaaaggat tagtagattc ttttaaacag aaaacctatt gatcattgaa
    20341 atttaaatac attctatcag tacgttaaca cttcaaggag catattttag ttgcgccatg
    20401 atttcttact gtaaagaagg agcgataaac attctaaaat aatttttata gtcaataaaa
    20461 agcgatgatt tgaagttggg ataatgatac ttccgtaagg ctcgccgtaa acttggagag
    20521 aggtgaaatt cctatgtgct tttgacaaaa gcagctgatt aagaattatt tccatatgtt
    20581 gatttcaaca ttttttctat tatttttaag accaaattca gtgaggcaaa tagcatcatg
    20641 taaatttagt ttttctattg aacgttctcc actttttagt ttttttatag tactcaaatt
    20701 taatcctgta tgtttagaga tctcatttgc agtatggttt tgtaaagttt ctcggattat
    20761 tgttatatta gcaatttcat tacgcataat tatgttcctt tcttatcaaa aatatcaatg
    20821 actacagtgg gcttttagtg aaaaaaaggg gtactacagt ctcttttgaa aacaaaaaaa
    20881 tacagtatat acgttttcta aatgctaagc accaacgttt acactgtatt taagggtttg
    20941 gcacccaatg ggctgtgagg ggctcgaacc cgcgacccga tgattaagag tcatctgctc
    21001 taccaactga gctaacagcc caaattaata ctttgttatt ctaacaccta aaataaaaaa
    21061 gtgcaatact tattatcaga attgagagtg aattttatgg attttcaaga ttatgaaaaa
    21121 atatttgaaa gttatccatc gatattgaaa attactgatt taactaaaat gttaaatatt
    21181 ggaaaaaata aagcttataa tttgctgaaa gatagatcaa tagaaaatat aaaaattgga
    21241 gcaaggtaca aaatacctaa aaaagctgtc attgagtata tattgaaaat acaagatgaa
    21301 ggtaaaaatc atgctataat aaaatagtca aaatttttgc cgatatttca gtttaagagg
    21361 agagattgaa ttgaaattaa gagcaaacat aattgaaaaa agaggaacat tacaagtagt
    21421 tgtatcttat aaagactcta acggaaaaat tagacaaaag tggaaagata caaaattaaa
    21481 aaagaaagga aataaaaaac gagcagaaaa aattagagat gaatttttac tagaattgga
    21541 aagtcaatta gctatggaat gtgaatgtgc taatccaaat ttattgtttt atgactattt
    21601 gcttaattat ttggaagtat caaaaaaaca agtagcattt aacacatatg ttgggtataa
    21661 gcattacgtc cataatcgaa tttacaagtt tttttatcct aaaaaaatta aactaaatag
    21721 gttaaagcct tatcatttac aagagtttta tcaatatatg ttaaatgata actgtacagc
    21781 aaatacggtt attcattatc acgcatttat acgcaaagct cttcaagagg ctgttattac
    21841 agaattgata agtacaaatg tagcagataa agtaagaaag ccaaaaaaga aacaatttgt
    21901 ttctcaagtt tataatcaac aagagatctt gaaattactt gacattttgt cagaagaaaa
    21961 actctatttg gtagttcttt taacagcatt ttatggattg cgaagaagtg aagttttagg
    22021 attaaaatgg tctgctattg attttttaaa caaaaaaatc atgataaacc atgtaatcat
    22081 agaaaatcca gagaatatgt cacagttaat aaagaaagat caaacaaaga actcttccag
    22141 ctatcgtaca ttacctttgg ttgaaacgat tgaacgagca ttagtaaatc aagctaaatg
    22201 gcaacaagat aatcgtattc ttttaggaga tgattatatt ttagaagata atgagtacgt
    22261 atttactatg gaagatggac ggttgatgaa gcctcaatat gtaacgcatc gtttgagtaa
    22321 gttgattaag aaaaatggat tgaaaaaaat tcgctttcat gacttgcgtc attctaatgc
    22381 ttcaataatg ttagatagtg gccagaatat gaaatccatt caagaatggc tgggacatgc
    22441 atcgtattcg acaactgcta atttatacac gcatttaacc gcaggagtaa aagatcgtgc
    22501 agcagaagca ttagaaaacg catttgggtt taaagaataa aaaaacaaaa aacaatatct
    22561 ttggggtatc gcttcttgtt tttagaattt ttttgttggc aatttgttgg cagtatgttg
    22621 gcaatgaaaa aattcacaaa ctaaaaacct atttataaag ctttataaaa cttttttcac
    22681 aaatagagca tttggttaag agtcagctgc tctaccaact gagctaacgg cccgtacaaa
    22741 aagtacttaa taatagtagc actaagaatt ttaaaatgca agactacatt taaagaaaat
    22801 cagaaaaatc tttacttcct tataggaaat cattcataga ttattcacat ttgctcgcta
    22861 ttatataagc ataccaacaa acaaagttct tgcaagagaa caaaccctaa cacttttttc
    22921 attttttact cctttcccgc cgtctccccg gcgggtctcc tgttcttaac gataaaagac
    22981 aagaaggctt agccttcttg ttttttgttt tcatggaatc ccgctaatca aatcacctag
    23041 ttaaagcaac tagattgaat gtttatcata agtctgatat catgtttatg aaatgaaaaa
    23101 aggagcggtt atgatggaaa aaatgcgtca gcaattaatg gagtggggga aggaactaga
    23161 aacattccgt ctgcctcact gggaagaact ccctgactta gaactttata tggaccaagt
    23221 cagaacgtta gttgatcgtt atctttcacc tgtgatacaa ggagaaaagc atccattgct
    23281 tacctcttct atggtaaaca attacgttaa actaggtttg ataccagcac cagttaaaaa
    23341 aagatataac aaagaacacg tagcattttt acttgcaatc actacgttga aacaagtatt
    23401 gactattcct gaaatcaaag aagggatatt gttccaagga aaaacagtag gaattcgtga
    23461 agcctataat ctattttgcg atgaacaaga agcagctgtg tggatggtca gccaattagc
    23521 acaaggtaaa tctcatccgc aaaaattcga ccaggcaacc cctgtagaat atattgctgt
    23581 aagagcggca actttatcgt ttgcaatgaa acttcttgcg gaaaaaacga ttgttctaga
    23641 aaccgaatat ttaaaagagg agaaaacaaa tgaaaaacaa taaaatcgca attttagtag
    23701 attcaggtac ggatgttcca gaagaattga taaaaaaata cgagatgtat atgatcccat
    23761 tgaaaattat ttataaagat caggtatata cagataaaat tgacattacc cctgaagaga
    23821 tctatcaacg tttgccagag gaaatccctg gtacatcttt gccggatggt gagtcgatca
    23881 cagaaatctt tgagcaaatc aaaaaagacg gttatgaaaa ggtacttgcg gtaacaattt
    23941 ccagtggatt gagcgggaca tataatgtgg ttcgtttaat tgcacaacag caagaagaat
    24001 tggaagcata tgtactagat acgaaaaata ttggtattgg tgcaggtttt tctgcgatcc
    24061 aagcagcaaa atggctggaa gaaggagtag aatgggatca actgatcagt aatctgaatg
    24121 agttagtcga aagaacaaaa gttttcttta atgttgcgac attggaatat cttcaaaaag
    24181 gtggacggat cggtttagtt gcttctattg taggaaccgc tttaaagctt aacccaatca
    24241 tctcatgtaa tgaagaaggg atttattaca cagtaggaaa agctagggga cgtaaaaaaa
    24301 gtttggataa aacggttgct tacgtcaaag aaaaaatcgg taatgcaaag gtcttcaact
    24361 tagctgttgc ccatggggat gcgaaagaag aagcaattga aatggaagcg agacttaaaa
    24421 gagagttccc acaagcaaac caaatctact ttggccagat ttctccggca ttagtagtcc
    24481 acactggtcc gggattattg ggtgtcggtg tacaagtttt agaaggataa atcaaaaaaa
    24541 ggaaacactg aggtgaaaat cagtgttttc cttttttatt gaagttagaa caaaaaataa
    24601 taaagtattt tgcactttca ttttaatttt ctcttttgtt ttataggcaa agatgaaaca
    24661 aaagcagaaa tacggtataa tgagaaggta tgacaataat atataagagg tggctcttta
    24721 tgaaaaaagt tttagttgtc gatgatgaga agccgatttc ggatatcgtc aaattcaatt
    24781 tagccaaaga aggctatgac gtttatactg catatgatgg ggaagaagcc ttggaaaaag
    24841 ttgctgaggt agaacctgat ttgattttac ttgatttgat gctacctaaa atggacggct
    24901 tagaagttgc tcgtgaggta cgcaaaactt atgatatgcc gatcattatg gtaacagcca
    24961 aagattctga aattgataaa gtcctaggct tagagttagg cgccgatgat tacgtgacca
    25021 agccattttc caaccgagaa ttagttgcac gtgtcaaagc aaatcttcgt cgcggtgcca
    25081 cggctgcgaa ggaaccagaa gaggcagcac ctgcggaatt gacgattggt gatttgacga
    25141 tccatccaga agcttacatg gtgacgaaac gaggagaaac aatcgagtta acacatcgtg
    25201 aattcgaact actcttttac ttggcgaagc accttggaca agtgatgaca agagaacatt
    25261 tattacaaac ggtttggggt tatgattatt ttggagatgt acgaacagtg gatgtaactg
    25321 tacgtcgttt gcgtgaaaag atcgaagata atccaagcca tccaaattat ttagtgacac
    25381 gtcgtggtgt cggttactat ttgcgtaacc cagaacagga gtaattggat gaaaagaaaa
    25441 gtccgctttt ttcgatcagt caattttaag atcgctatta cctttattct gattttattg
    25501 atctcgatcg aaattattgg tgcctacttt atacgtggtt tagaacgttc cacgatcaat
    25561 acatttatta aagatatgaa tcaaacggtg gaatcattag caacaacaat cagcccggaa
    25621 ttgaatcgaa aagataatgc agatgatgaa gaggtaaatg cgaatatcaa aagatttatt
    25681 gaaaacagtg cgactagcga tatcatcgaa atacgggtcg ttgacgaaaa agggatcatc
    25741 cgaggaacaa ccgatgtcaa tgaacaaagc gctgtgggaa agaaaaatga ttatgttgat
    25801 atcaatgatt tcacgactaa acgttatgtt gctttagata acgacgataa acgggtgaat
    25861 atcaatgttc aaccgatcct gtccccgacc ggcgatgccg ttatcggggc tctttacgtc
    25921 aaaagtaata tcgaacaaaa atattcggaa atcaataaca cagcagtcat tttctttact
    25981 gcctcactaa ttgcggcatt tatttcaatg atcgtatctg ttttagttgc tcgttcgatt
    26041 acacagccga tcggtgaaat gagagaacaa gccattcgaa tcgctagagg agactacagt
    26101 cggaaagtca aagtgcatgg acaagatgaa cttggacagc tggctgatac cttcaatcag
    26161 ttagctgaac gtattgaaga agctcaagat acaatggaag ctgaacgcaa tcgtttggat
    26221 agcgtgctat cccatatgac agacggcgtc attgcgacag atcgcagggg taaagtcatt
    26281 acgatcaatg atatggctgt ttccttgcta gatatcaaaa acgaggaagc aatcggacaa
    26341 tcgattttga cacttttaga tatcgaagaa gaatatacgt tgcggaaatt attagaagat
    26401 ccaaacgaaa tggtacttga ccgttcaacc agtacattgg aaagtgatca gatgatcttg
    26461 cgagtagact tttctatgat ccgtcgcgaa tcaggtttta tcagcggatt agtagctgtc
    26521 ttgcatgatg tgacagaaca agaaaaaaca gaacgggaac gtcgtgaatt cgtctctaat
    26581 gtttcccatg aacttcgtac accgttgaca agtatgcgta gttatatcga agcattgagc
    26641 gaaggtgcgt ggaaagacga ggaagtagca ccaaattttt tgaaggtaac gctagatgaa
    26701 acagaccgaa tgattcgaat gatcaatgac ctcttaaatc tatctcgcat ggatacaggt
    26761 aatacccagc ttcaattaga atatgtcaat ttcaacgagt tagtgaattt tgttcttgac
    26821 cggtttgata tgatggtggg aaatcaggaa aaaaattata agatccgtcg tgaatttacg
    26881 caaagagatc tttgggtcga agtagataca gataaaatca tccaagtagt cgataatatc
    26941 atgaataacg cgatcaagta ttcaccagat gggggaacga ttacttgtcg cttattagaa
    27001 acgcataata acgtgatttt aagtatcacc gatcaaggat tggggattcc taaaaaagat
    27061 ctgaaccgag tgttcgagcg gttctatcga gtagataaag cacgtgcaag agcacaaggt
    27121 gggacaggac ttggattagc tatttctaga gaagtgatta aggcgcatcg aggcgctatt
    27181 tgggcagaga gcaaggaagg aaaagggtcg actttttata tctcgctgcc atatgaacct
    27241 tatgaggagg aatggtggga atgagaatct ctgaaaaagt cgttcgtata ggactcattt
    27301 tgatggttgc gttaagcata tacttctctt atgcaatctg gctgagccca gcaggaaaaa
    27361 gttcaatcaa cgtagatgag acaaacactc aaatgatcga gtctcaaagc aatcgaaaaa
    27421 cttccgaagt ctttcttccg cttcatgtta catggcacca ttcgggaatg atccaagaaa
    27481 ccaacagtga aaatctcgtg taccgattgc aattgatcat cgataaagca agatttggaa
    27541 agctttctga agtagtttca ggagacaaat cacagtttga acaggtgaaa aatctgaact
    27601 ctggttttga gttggcctat aatgcgcctt ttttgctaag agaatacaag gaagcatttg
    27661 atttagattt agatgtgtcg aacttggcaa aagaaagcga aaatatgtat tttactagaa
    27721 tacaattcga tcaaggaaat aaaaaaatcc gatttttgaa ttattcgaac catctcgtat
    27781 atgaagcaaa tatttcgttg aattggacag atatcaataa agaattaaca gcaaaagaca
    27841 caaaatggac agaaatgaca acagaaccat cgacgatcga cacgcaatat gctacgaaac
    27901 attcgatcaa actgaagaag tatagttata tcctgtcaca gcaaccctat acacttttta
    27961 gaaatgcttt tttccagaaa ccagatagcg tgaaaaataa tgatgaaagc aatgaattga
    28021 ttttttacgg tgggaatgaa acaatgtcta tccattcgga aacgcaagta gttgattttc
    28081 gtggagagct gccagagaaa aaagaaaatg ctagtgtatt ttctgaaagt ttcccctata
    28141 ttagcaaatt aggcagttct ttagggaacc tcagatattt tgaccgcagt gacaatacga
    28201 tcgattatcg gatattcgtg gaaggcttcc cggtttttgg gacagacagc agaggacaga
    28261 ttggtgtgga agttggcggt gaaacgaatg gttctgtgca agtgaatatc caaaccagtt
    28321 tgaatacgat tcaagtgccg ataccttcag acgaagaagt agaacttgca agtacgaatg
    28381 aaatcatcca gcagttaaaa gctaagggag cagatagcaa caagattggt tcagtgatcg
    28441 ttggctatac atggcaaaat gtcaaagaaa cgaatcgtgt cgttgatctc ttacctgaat
    28501 ggtatgtgaa atataaagat aactggtatt cagctaacga ccttctggaa aaattacctg
    28561 gattggaggc gaattaattg gatttcaaaa aaatcgaatg gatatttttc gtcgcttttt
    28621 tagggttgaa tgtgttttta ttcagcagtt atcatgacgc ggtataccaa gaaaacaatg
    28681 tcattcgctc taatcagact gaatcaatcc aacaacgtct ggctagcgac gatatcactt
    28741 acacggggaa gatttcgtct gagaacaagc aaggctatta tcttagcgcg gagcagacca
    28801 atcttagcac ttcattagcg aattatcgca aggctggtaa aagcggcttg ctggcaagtg
    28861 gtgtgttttt agacggtgat gttttgacga aatctttatc cagttcagat gctgaaaaga
    28921 acgtgatcga tccagataaa attagtagtt ccttggaaga ttttttatct aacaaagaca
    28981 atattttatt cggtaatgat tatgtgtaca cgaaaaactt ttctcattta gaagaagaac
    29041 cactagaaat ccaagcaagc caagagtatg aaggcatccc attcaatgat gatacagcaa
    29101 aaatcgttct ttctttagaa aaaaacgata atgattatag catcacgaaa tacatgcaga
    29161 cgcatttatc tgatatcgag cagctgcgcg aaaagacgga actgtatacg gaagaggaag
    29221 caatcaatac attgtacatc aataacaaaa taccaagagg atcaaaaatt ttatggcgtc
    29281 agctagctta ttcctgtatt ctgaaagtcc gggaaaaaaa tgtgtatgtt cctgtttggc
    29341 atgtagcgat cgaaacaagc gataaagtgg tccaaattga gagcgtcaac gctttcagca
    29401 atacgatcgt gacaaacaat actgtaccaa aagtggaaga tcaataaaaa agcgagtata
    29461 atgaaaaaag tttacaaagt aagaagggaa gtaggagtca aatggctgaa gaaaaaccag
    29521 cttttaaaat cagtatccta gcaagtggaa gttcgggtaa ttcactttat atcgaaagtg
    29581 aaaaaaaacg gcttttggtc gatgcaggac ttagcggtaa aaaaatcact tccttgatgg
    29641 ctcaaattga tcggacaccg gaccaattag atggaatact tgtgacgcat gaacatcgag
    29701 atcatataca tggcgtgggt gtcttggctc gtaagtatca tttggatgtt tttgccaatc
    29761 agcagacttg ggaagcgatg gaaccactga ttggaaatgt accagtggaa caaaaacatc
    29821 tatttgagat ggggaaagtc agaacattcg gcgatatcga tattgagagt ttcggcgtct
    29881 cccatgacgc agcggctccg caattttatc gtttccataa agaaaatcgg tcattcgtca
    29941 tgttgacgga caccggatat tgcagtgatt atatgcgagg tgtcattgaa aatgccgatg
    30001 gttatttgat ggaaagtaat catgatcttg agatgttacg catgggacct tatccgtgga
    30061 atttaaaaca gcgtatttta ggagatagag gacatctttc caacgaagac ggtgcacttg
    30121 tcatgactga tgtgatcggt aatcggacca aacgaatcta tcttggccat cttagcaagg
    30181 aaaacaatat gaaagaactt gctcatttaa caatggaaaa tgttttgaaa gaaaaagatt
    30241 ttggcgttgg acatgatttt gaaatctatg atacagatcc agatgaagca actgagctat
    30301 tttcgattta aacagttcaa tcaataaaaa tataaaaagc agaagataaa atcaatcagg
    30361 gttggtttta tcttctattt ttattttttt agcatattaa aaaaataacc gtattatcga
    30421 ctgactacac tgatgtgctg cttttcatgt ttctcatcca gtatctcatc tactaatgca
    30481 atcgcgtaat ctgccatgct gatcgtactg tcgctagcag cgtttctttg taaataatca
    30541 tcacttaaca aataagtacc tgtacgtttg ccatgtggat caaataaaga agctggactg
    30601 atataagtcc aatgtagatt tttagattgt ttcaatttta acaatccttc gtacatattg
    30661 aaagctgttg gatagaaggg tgcattttta ggtacttggt caatcatacg agtcgtcttt
    30721 tctgtatcta agaataacga agaggcaccc ccaacgacaa ttaagcgtgt atcggttcct
    30781 tcaagcatct caatcaaatg ggacaaagaa gtttgatgca tttcttcttt tcctttggga
    30841 gcgttgaaag catcaacgac aacatcaaaa gatgcaagat cagtagttgt caactcaaat
    30901 aaatcctttt gtataaacgg aacatctgat ggcaattttt ctggatgacg cacgattgcc
    30961 gtcacgtcta aatctctgga caatgcttcg tctaaaataa atgaacctgc atggccagta
    31021 gctccaatga gtgcaatttt cataaaaaac ctcctttttt attcctgtcc attgtaagca
    31081 gttgctgaat attcgtcaaa gaatccgatt tttgaaaagc tcagtgcgca ttcctttcat
    31141 ggttcgtgat cattgggtat agttaaatta agaaaactga ttgataaaaa tagaagttga
    31201 aggagataag aaatgaaaga gtggttagat cgtgcctttt taccaatgat aacatctgtt
    31261 gtgaatctta gacccttaac tgctttgcgt aatggaatga cttacacgat cccaataatt
    31321 attgttggct cgctgttttt cttatttgct cattttcctg tggattacat cgctcagtgg
    31381 atagacaaaa caggattttc tatgtatttt acacaagtat accaggctac atttgatgtg
    31441 atagcctttt ggatcgtttt tgccattgca tacgcttatg caaaagaaga agacatcgat
    31501 cgcttaccag tcggcataac agccttgtct gcttatctac tgcttattca gccactaaat
    31561 actcagatag atactgtctg gacaggtgct gaagggatac taggagcagt tacaactggg
    31621 ctcatcatcg gatggggcta tgcacgttta gtaagacttg cgaaagggtg gaagcaaaga
    31681 gagaagatac ctgaaaatgt ttggcagtct tttacttcat tgattccaat cattgttata
    31741 gtcactttta cgcttatcgg ttcagcggtt tttcaacatt tgttcagaat gactgttgtt
    31801 caaggagtat ggacatatgt ccagcttccc attcaatcgt tcatagacac gttgtttggt
    31861 gtgcttttat taggtttttt tgttccgttt ttatggttat ttggtcttca tggatctgcg
    31921 atggtcaatg ggttggttag tcccgtcttg caagcgaatt cattagcaaa tgcagaaatc
    31981 ttagcctcag gacaagagtt gacggtggca aatggcggac atatcgttac tcagcaattt
    32041 ctcgatcagt tcatgacggt tacgggtgct ggattgacgt taggggctgt attttttatg
    32101 acggtttttg cgaaatcgaa gaaatacaga gaattaggaa atcttgctct tttaccagcc
    32161 tttttcaaca tcaatgaatc aattattttt tccacaccga tcgtgatgaa cccgatgatg
    32221 gctgttcctt ttattttcgc accggtcgtt tcaggattga ttacctattc cgctttatat
    32281 tttggattcg ttcctttatt tacagcagtc caagtccctt ggacaacacc gccgatcctc
    32341 tcgggtttct tagtaggcgg agtgcctgca gcgattttac aaggtatcgt gttgtcaatt
    32401 agttttttca tttatttccc atttttgaaa aaaatagatt ctgcaaactg caaaaaggaa
    32461 agacagacaa aaaatgatgg agcagcagaa aagatgaacg actaaaaaac aaaaatactc
    32521 ctgtcgcttt agattgggaa aatcaccaat ctaggatgac aggagtattt atagtcatgt
    32581 ttatactagc cctttttgga cagctaattg aacaagaaca actaatgtgt tcattcctac
    32641 gtgtgtgatc atcgatgtcc agatccgacc agttgctttg tactctaggc tgaagaagaa
    32701 acctaggaaa aagtagatta gcagatgacc atcattatga gcaaacgcaa acagcaaaga
    32761 gctgatcact gctccgaccc aaaaatttga atatcgactg atgatcccca gtatactgcg
    32821 gcggaatacg aactcttcca tgatgggtcc agcaacagtt gtcgccaaaa taaaagcggg
    32881 tgcttccatg atcatctgga tgatattttg tgtattctcg gaaggggttg ctttgccaaa
    32941 caatacagat tctaacatga cagcgatgct ttgcagaatc aatgcgacaa agattccaac
    33001 caatccataa ataacaatct ttggagcaga agcttttttt accgctgatt cgatcgctaa
    33061 gggttcacgt tgcttaaaat aaaaaatagc taggacaacg gcgcctagta tatacatgac
    33121 tgcagttgct gtgatcaatt cagacgtaga ttttacaagt cctatcgaag ctaggatcaa
    33181 gggtgagata aaaattagac catagcaaaa aatgctgagg aagctatatt tttttaaaga
    33241 catgtaattc ctccttttaa gggataacaa gcatgcaagg caagcttata tcgattgatt
    33301 tcaattatat cataatttca ataagcagct gtcagaataa aatttctttt ctttgattta
    33361 aaagcttgca aaaaagaggg agaatgagta ttattaaaaa tgtaggttag cactcgaagg
    33421 ttaagagtgc taacagatta aaaaaagagt tatcggaggg atttatcgtg ttaaaaccat
    33481 taggtgatcg cgtcattatc gaagtcgcaa aagaagaaga aaaaactgtc ggaggaatcg
    33541 ttttagcttc tgctgctaaa gagaaacccc aaactgggac agttgttgca gtcggtgaag
    33601 gccgtttgtt agaaaacggt gaaaaagttc ctgccgctgt aaaagcaggc gaccaagtta
    33661 tgttcgaaaa atacgccggt acagaagtaa aatacgaagg aaaagaatat ctgatcgtcg
    33721 caggaaaaga tatcatggcg attgttgagt aaatttggaa gataaaaaaa taatcaaaaa
    33781 taatcaatga ggtgaatcaa tcatggcaaa agaattgaaa tttgcagaag acgcacgcgc
    33841 agctatgtta cgcggggtag ataaattagc agatacagtg aaagtaacat taggacctaa
    33901 aggccgaaat gtcgtactag aaaaatctta cggctctcca ttgatcacaa atgatggtgt
    33961 gacgatcgca aaagaaatcg aattagaaga tcattttgaa aacatgggtg caaaacttgt
    34021 atcagaagta gcatcaaaaa caaatgatat tgcaggagac ggtacaacga cagcaactgt
    34081 tctgacacaa gcaatcgttc gcgaaggttt aaaaaacgta actgcaggtg ctaatcctct
    34141 aggcattcgt cgcgggatcg aattggcaac aaaagcagca gtcgaagaat tgcataatat
    34201 ctcaactgtt gttgattcaa aagaagccat tgcacaagta gcagctgttt cttcgggttc
    34261 tgataaagtt ggacatttga ttgctgatgc aatggaaaaa gttggaaacg acggtgtcat
    34321 cacaatcgaa gaatcaaaag ggatcgaaac agaactagat gttgtagaag gtatgcagtt
    34381 tgatcgtggt tacttatcac aatatatggt aacagataac gacaaaatgg aagctgttct
    34441 agaaaaccca tatatcttga tcacagacaa aaaaatctct aacatccaag atattctgcc
    34501 attgttagaa caaatcttgc aacaatcacg tccattgttg attattgctg acgatgtaga
    34561 tggtgaagca ttgccaactc ttgtattgaa caaaatccgt ggaacattca acgtagtagc
    34621 tgtaaaagct cctggtttcg gcgatcgtcg taaagcaatg cttgaagata tcgctatttt
    34681 gacaggtggt acagtaatta ctgacgatct aggtttagaa ttgaaagatg caacaatcga
    34741 aaacttagga aatgcttcta aagttgttgt agataaagac aatacaacga tcgtagaagg
    34801 ttctggagaa aaagaagcaa tcgaagctcg tgtgcaattg atcaaaaacc aaattgctga
    34861 aacaacttct gattttgacc gcgaaaaatt acaagaacgc ttagctaaat tagcaggtgg
    34921 tgtagctgtt gttaaagttg gtgcagctac tgaaacagaa ttgaaagaat taaaattacg
    34981 cattgaagat gcattgaatg ctacacgtgc cgcagtagaa gaaggaatgg tctcaggtgg
    35041 tggtacagct ctagtgaacg ttatcagcaa agtttctgct gtagaagcag aaggagacgt
    35101 ggcaacaggt atcaagatcg ttgttcgtgc tttagaagaa ccaattcgtc aaatcgctga
    35161 aaacgctggt tatgaaggat cagtgattgt tgataaattg aagaacgtgg aactaggcac
    35221 tggtttcaat gctgctactg gagaatgggt aaacatggtt gaagcaggta tcgtagaccc
    35281 aactaaagta acacgttctg ccttgcaaaa tgccgcttcc gtttctgcct tgttattaac
    35341 gacagaagca gtcgttgcag ataaaccaga accagcagcg ccagcagctc ctgcaatgga
    35401 tccatcaatg atgggcggta tgatgtagta attaagaaag aaccctattt taacagggtt
    35461 ctttcttctt tttttgtttt attttaagca aaaggggcaa agaaggggca aaaatatcaa
    35521 aggctattta gtttatcaat tacttgttgt ttggctcttt ttgttacatg gttataaata
    35581 gacagagttg tgtttgcatc agaatgccct acacgctcca taatggcttt aagaggtacg
    35641 cctaattcag ataatagtga aacgtggcta tgtctgaata tatgtgagga tagacttttt
    35701 tctaattcca actcttcctc tactttatgg agtatagcgt taaatgagtg cagcgcaagc
    35761 ggagtgccac ttgtagatat aaatatatat tgatcgggat ctgtgggtct acctgcaaga
    35821 atattgtcag ctatcacact ttcaatcaat tcttttgcac gattgggtaa ttgcacttca
    35881 cgttgcgaat aagtattttt tggagttgtt tttatagcat tatccatttt cacagatgtg
    35941 taatctaagg tcccattaat ggaaattttc ccatcctcat agtccttcat ttgcaaagct
    36001 agcaattccc catatctcaa accagttaaa tatagaaact cagctattat gccgtgtagt
    36061 tttctgcgag gattggagta tagctgtttc agtatttgat caatttcttc tttctccaga
    36121 tattttttat tcatagaaag ccttcttttt tcttcttcca cttttttagg atggatttta
    36181 actgctagcg cagggtttct ttgaatgtat tttctatcga ttgcatagtt tagcataaca
    36241 gatagagttg tttttgtttg ttttgtgtag ttcagtgaga ggtcaccaaa cgtatacata
    36301 tcttcaatta tcttattaat gagtgtctca tcaatgtttc taacgatcgt atcatcgctt
    36361 atgtgcttag aaacatgttt catcatcatt ggaaccttca aatagctagt acgtttaaca
    36421 tgctgcttat aatattcata ccattcttta tacagctcac caaaagtgat atctgattta
    36481 ttgtaatctt cgagtgcttc tttaattttt ttatctagaa ttttctgagc ttttttccac
    36541 gcttgtggtg aattacttgt aagtgttgta gatttttttc gtgttttttc tgtataagga
    36601 tctacatatc tttcaataaa cttgaattgg ccatctttgg tctgttcaac ccacactttt
    36661 aacatctcct atcatttgct ataataggca taacaaatag acctatatag gtttgttttc
    36721 taaaagcacg ctcttactct ggacggtggg gcgtgttttt tatttattat acagttgctt
    36781 taaaaatagc tgctttttca aatgatacta aaggagagaa gtggatttct atttctccct
    36841 tttggtttaa cgtgaaatga gcagttacat ccatttgttt tcctggagca acagatccca
    36901 ttgtattttc gttggcatat gtctctgatt ttttgtcatc tggtccatat acttccacat
    36961 ctgtacctac agggatatct gaatcgccat cattttttac ggtataagta atttttacta
    37021 cttgtgcagg ttggttttct tcaaattgat ttctttcatc agttagttct acactattta
    37081 gtgtatattc agcatcacca acttttacag tgtcaccaat cttatagaag gtatcgcttt
    37141 tttcttcttt agaagatgaa gcagaggttg aagatttagt tactttttcg ccaccattat
    37201 cacttgcttt attactatct gatccaccat ttaaagcaga accaataatt ataattaaaa
    37261 ttactgctaa tacccaaaac catacttttt tgtaaaaagg tttactaact ttatacattt
    37321 ttccgtcttg acccataact ttttttgcca tttaaatatt cctcacttct tgttataata
    37381 tatttgcgat ctcagaaatg aggtatgagt ccgtgtgcca gcacgggctt ttttaatgtt
    37441 ttgctgaaat cggttttttt aataattctt gatgtattca tacatattgc cttgcgtaag
    37501 aatatttttt cttaaaatag cattggcaga cagcataaca agtgtatctg agcttattct
    37561 gaatagaata atactccata aatttttcga gattaaactg agattcatca gtcagttcgt
    37621 tctcaatata gatatttaat aaaattagaa tagctatttt atctgcttct gtttcaaatt
    37681 ttgagtgaaa agttgtagaa gtatcgtaca aaactgaata ttcaaaatga gaagcggtga
    37741 aatgcgcaag ttcatgagat aaatgaaagg cttctgcagt ttcgccatat agattttcat
    37801 tcaaaaaaat gattctaggc ttcggataat agaagccagg ctctttcatt tccatgtaaa
    37861 ctactttcaa attgtattcg ctcaacattt ctttcaattt caaatacata caacccatca
    37921 ctccaactat tcattttcct ctaaagcttt agcaattgca atcgctttac gcattgtctc
    37981 cttagatatt tcttttccat caaaagaaaa aacagtatcg ttttctgata aatccacatg
    38041 tttaggggtt tctctttctt ctctacctag aaggtagtct acagagactc caaagtaatc
    38101 agctaaccga gctaactcaa tagaattagg agtttgtctt ttccaacttc ctaaatatcc
    38161 attcgaatac ccgaaattca tttcgagctg tctaatggat aatccttttt cttttgctaa
    38221 ctcctttatt atttcgtaag tattcattga taaatcaacc tttctgaatg cttacaaaaa
    38281 aagtttagat aaataatgta aaaatagttg acttatatac attattaatc tatactatgt
    38341 tttgtaaaca agtttaacaa ctaaaaagac aacaaaaaat actattgatt aataaatgcc
    38401 aaccgccaag aaagcttttt aaatcaaagt ttatatgtct tatttaacta tgcatttagt
    38461 atagattatt aatctaataa agtcaacgca gttttttaaa aagttgttaa atttgtttac
    38521 gaatataaaa gaaaggagag aagaatatgg aaaaaacagt ctcggcaaaa atcgaagatt
    38581 tgaaattaga catactgaaa caagcaaaag tggcgatgga acacgcggta gataaagaag
    38641 actctgccat ggttgcagcc atagcagaga ttttagctca cgtttagtca tcgtttttac
    38701 ggtcttctgg aagctgacca tagataacag cataatgaat atctaaataa gcttgaacaa
    38761 tttcttttgg cgaaattgat tcgcctttag tagatacttg tgattcgtga tttagccatg
    38821 caacaactaa atccgcagcg atttcaaacg gcgttacact ttgtgtcagt tgccataaag
    38881 aatttcacaa tatgtatggt cgtggtaata ataccaaaga gcaatttgag gaatttatgg
    38941 aaattaaaaa atacaaaaaa tcatttatta aaggaggcta aacaatgaac aaggaaatga
    39001 gccaaacaat caaggtacaa aaaatgatag atgatctaac acatggaatt gatagccaag
    39061 ccgataaaat catcaaggaa ttgcaaggac aaaaagttaa ggatgcaaaa atgcttctca
    39121 agactatcaa ctttgaaatg aatccaacaa aaagaaagct cgccgatgtg ttggaagaaa
    39181 agttggcttc cgctatcaac gagcaagaat tactatttga aaccgatact tttaacagct
    39241 gatgttttat agatgacagt ttcatccgaa tctatatcat cgccgagaga aaagaatgtt
    39301 gcttttgaca aaaagccgat aagacctaca agcggtcttg atgttgcaag agtagatacc
    39361 ccaatatcac cttgcaaggt attcacgtaa atttgagaag aatagaacat tccatctttt
    39421 tcttcgatat ttgacgcatc atcgtatcca acgaccaaag tatcggcaaa tattttgatt
    39481 ttttctccat caagcatagt aattacaaat tctggtttca taaattttca cctccttaac
    39541 aattatttca gcctgtcaca ctgataagga aattatacca aagaaaggaa tgagaaatat
    39601 gaatacacca caaattttta atttcgagca aaacgaagtt cgaactattt tagtaaatga
    39661 tgaaccatat tttgtaggaa aagatgtagc agacgttcta ggatattcga atccgcaaaa
    39721 agctattcga gatcatgtgg acttggagga taagacgcag aacgattcgt tcaccgtcaa
    39781 tggaacagca gttgttttga ttaacgagtc aggcctttac agtttaatct taaaatcaaa
    39841 acttccctct gccaaaaaat ttaaacgttg ggtaacgagt gaagtccttc caacaattag
    39901 aaaaacaggt agctattcaa acgtacctca aagttttgca caagcattgc gtttagcagc
    39961 ggatttagaa gaaaagaacc aattactcga acaacaaatt gccgagtacg aaccaaagat
    40021 tagctactta gatacgattc tttcatcgac agatacggta gctacttctc aaattgcagc
    40081 tgattacgga atgtcggcaa ttgctctaaa caaattgcta aacgagttag gtgttcagca
    40141 taaagttagc ggacaatgga tactttaccg aaaacatatg aaccaaggat acacaaaatc
    40201 gcacacaagt gagataccga aagccgatgg cggcactaaa gttgtaatga ataccaaatg
    40261 gacacagaaa gggcgagtgt ttatttacaa cttattaatc gcagagggct attaccctca
    40321 aatggattta gaggaaattg gttagaaagg agttttagta tgactgacat tgcagaaatc
    40381 actcaacgag atagagaaaa aatcaaagaa tatgtcgaaa gttcaaagtt cttaacttac
    40441 accatgcttg ctgaaagatt tggaattagc aaaagctact tatctttaat tttaaacggt
    40501 aaaaagactt ctgcagaagc aaacagaatt atagattcga ttatcactat gtacgaattg
    40561 tagagggagg taaagtcgtt ggaagataaa atcattctta caaaatcaga gcttcaagaa
    40621 atgttaaaca tcgagtatgg aacgataatt tttaggaggg ataataagtg aaaaaaccaa
    40681 cactttcaga gctgatagaa gctgctgaga aggcagtaaa accagacgac tggtaccgac
    40741 aaagtttaat cttggagaag ttccacggca tgtcaaaaac tactttagtt gaatactgca
    40801 aggaaatgga aacaattcct gaatttgcag aaggaatact tcgtccaggt cactcaacca
    40861 catttattca ttaccatact tttatttggt ttttaaagtg gaaagacgca aacaaatatc
    40921 gtgtaaaaag tttatcgcct tcagatgttt tgaaggaggc aagttaatgg gcaaattcaa
    40981 cagagcacta gtattcagcg caccgctaat catctacgct ttaggcctct ggggaagcag
    41041 acaagcgttg atagggacaa tcgtttacat ggtttggatt tttatggggc ttgatgaagc
    41101 tgagcacaga gcgaaaaaag ccagtcggga gggactgact aaaatatgat ttttttattt
    41161 ttgttccttt gggctgctat tgcgtacttc atccagctcc ttgttgtcaa agttgctctt
    41221 atttctttta ggaagaacaa aagaaaatag cgctataaat gaaagataag tagccgctac
    41281 attaaactgt tcttttgaaa aagttacagt catcggaatg ataattgata ttgaggaagt
    41341 aatcttaatt aaaaatatag caatattttc aacatcgcgt tggtttttat ttaacattgt
    41401 ccaacatgtt ttagcaacaa taaaaataat tacacatgat attagcacac agactgaaaa
    41461 aagttttaga aatgcgatgt agtcagtgtt attgaactcc ttcaaaatac tatttttatt
    41521 caatgtaata aaaggaattt ttttaattga agacaggaaa gataaggtaa taaacagaat
    41581 tatatatatg gttaaagtcg cagtaagaat gtaattgagc tttttcctca tagaatcacc
    41641 tcactttcta acgaaaatta taccaaaaag gagagaagaa ataatgcaag aattagtaat
    41701 tttgaaaaat aaagaagctg taactacgag cttacaagtg gcagaaagtt tcaaaaagaa
    41761 acataagcat gtactagaag caattgaatc aataaaaaga tcggtcgaaa attcggccaa
    41821 tgttgaagat gggtccaatt ttggacagat gtttgtggaa gggaacgagc cagactcata
    41881 cggaagaagt cggagagttt atttcatgaa tagagacgga ttttccttgc tagctatggg
    41941 attcactgga agtaaagcaa taaatttcaa actaaaattt attgaagctt tcaacgaaat
    42001 ggaagatgtt attcggaaga atactgttcc tcaaacaatt gaagacatga tgatctatca
    42061 attagaagaa atgaaagatg ttaaaaaaga tgtttccatg cttaaagata ctatgcgaat
    42121 tagcggacaa caagagtttg aaattaagca aaaaggaaat atgaaagtta tggaagttct
    42181 agggggaaaa gaaagccgag cttatgaaga aatcagcaaa aaagtattct caaaattttg
    42241 gtctgaattt aaacgtacct tttcaatccc aagatatggc gagttacctc gtaagagatt
    42301 cgatgatgct gtttcattta ttgaaatgtg gttaccagaa actgcgatcc gcatggaaat
    42361 cgatcaactg aacagacaac aaagactttt cggtgatgaa aatgaataga gctgaagcgc
    42421 taagaatagg gacggtaatt gctaatcgct ggtggagaca caataaacca aacatcctaa
    42481 gccaacaaca tatcgaaaag caaaaagcat ggcaacaaat aaaaaagtga ctcagccggc
    42541 aagcatagag tcacaaaaca tatctaagga gaattttagc atatgaataa agaactttcc
    42601 actttagatc aatatttgac tgattctgaa tggggcaagt cgaatatcaa ggaaacaaat
    42661 aatcgaaaaa tcagacgaaa tcttttgacg aacgaagaac tatcatgtga tcaagatgac
    42721 ttgggaaatt ttgtgagtat ttgggatcat gtttacctta tccatctatc aaaacattca
    42781 aataaacctg aatacatcta tgtcatcgaa gatggcttga ttgatgcgct agaagagtac
    42841 aacagagata acttgattga tatctcttat tacggatcag gtaagaaata cattgctgaa
    42901 atggaggcag aatttgatga gtgaaatcaa agggacaacg aactttgaaa aactttttag
    42961 tcgtaagtta aataaaattc tcaagaaaaa aggaaatttt gattatttat cttgggctca
    43021 cgcgtgggag attatgaaaa agaatgatcc acaggcaacg gtaactatta atgagtataa
    43081 acactacagg gttgtttctg gaactcatca agactttctt gttgaggaat ataaaccttt
    43141 tcttatggat gaaacaggga cttatgtatc tgtctcagta acggttaaag gacatacgga
    43201 aacaga
//
LOCUS       DAJSAW010000023        39177 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJSAW010000023 DAJSAW010000000
VERSION     DAJSAW010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.023"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(16..1380)
                     /locus_tag="ONT64_001319"
     CDS             complement(16..1380)
                     /locus_tag="ONT64_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR2302745.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1377..2936)
                     /locus_tag="ONT64_001320"
     CDS             complement(1377..2936)
                     /locus_tag="ONT64_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2302746.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDCPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(2992..5472)
                     /locus_tag="ONT64_001321"
     CDS             complement(2992..5472)
                     /locus_tag="ONT64_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302747.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5456..5791)
                     /locus_tag="ONT64_001322"
     CDS             complement(5456..5791)
                     /locus_tag="ONT64_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302748.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5805..6839)
                     /locus_tag="ONT64_001323"
     CDS             complement(5805..6839)
                     /locus_tag="ONT64_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCR2302749.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(6929..7609)
                     /locus_tag="ONT64_001324"
     CDS             complement(6929..7609)
                     /locus_tag="ONT64_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR2302750.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(7893..8996)
                     /locus_tag="ONT64_001325"
     CDS             complement(7893..8996)
                     /locus_tag="ONT64_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCR2302751.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(9625..10095)
                     /locus_tag="ONT64_001326"
     CDS             complement(9625..10095)
                     /locus_tag="ONT64_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HCR2302752.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            10425..12065
                     /locus_tag="ONT64_001327"
     CDS             10425..12065
                     /locus_tag="ONT64_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCR2302753.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            12219..13541
                     /locus_tag="ONT64_001328"
     CDS             12219..13541
                     /locus_tag="ONT64_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HCR2302754.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(13609..14043)
                     /locus_tag="ONT64_001329"
     CDS             complement(13609..14043)
                     /locus_tag="ONT64_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2302755.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(14108..14945)
                     /locus_tag="ONT64_001330"
                     /pseudo
     CDS             complement(14108..14945)
                     /locus_tag="ONT64_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(15514..15819)
                     /locus_tag="ONT64_001331"
     CDS             complement(15514..15819)
                     /locus_tag="ONT64_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCR2302756.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(15844..16275)
                     /gene="ruvX"
                     /locus_tag="ONT64_001332"
     CDS             complement(15844..16275)
                     /gene="ruvX"
                     /locus_tag="ONT64_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR2302757.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(16272..16538)
                     /locus_tag="ONT64_001333"
     CDS             complement(16272..16538)
                     /locus_tag="ONT64_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCR2302758.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(16793..17518)
                     /locus_tag="ONT64_001334"
     CDS             complement(16793..17518)
                     /locus_tag="ONT64_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCR2302759.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(17879..17949)
                     /locus_tag="ONT64_001335"
     tRNA            complement(17879..17949)
                     /locus_tag="ONT64_001335"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17915..17917),aa:Gly,seq:ccc)
     gene            18066..18818
                     /locus_tag="ONT64_001336"
     CDS             18066..18818
                     /locus_tag="ONT64_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCR2302760.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            18834..19313
                     /locus_tag="ONT64_001337"
     CDS             18834..19313
                     /locus_tag="ONT64_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCR2302761.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(19564..21144)
                     /locus_tag="ONT64_001338"
     CDS             complement(19564..21144)
                     /locus_tag="ONT64_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCR2302762.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(21137..21880)
                     /locus_tag="ONT64_001339"
     CDS             complement(21137..21880)
                     /locus_tag="ONT64_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2302763.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(22056..22832)
                     /locus_tag="ONT64_001340"
     CDS             complement(22056..22832)
                     /locus_tag="ONT64_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2302764.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(23080..23916)
                     /locus_tag="ONT64_001341"
     CDS             complement(23080..23916)
                     /locus_tag="ONT64_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HCR2302765.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(23927..24856)
                     /locus_tag="ONT64_001342"
     CDS             complement(23927..24856)
                     /locus_tag="ONT64_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR2302766.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            25048..25401
                     /locus_tag="ONT64_001343"
     CDS             25048..25401
                     /locus_tag="ONT64_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR2302767.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(25475..26131)
                     /locus_tag="ONT64_001344"
     CDS             complement(25475..26131)
                     /locus_tag="ONT64_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCR2302768.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            26357..28291
                     /locus_tag="ONT64_001345"
     CDS             26357..28291
                     /locus_tag="ONT64_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR2302769.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(28377..29600)
                     /locus_tag="ONT64_001346"
     CDS             complement(28377..29600)
                     /locus_tag="ONT64_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR2302770.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(29594..31492)
                     /locus_tag="ONT64_001347"
     CDS             complement(29594..31492)
                     /locus_tag="ONT64_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HCR2302771.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQISEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(31486..32604)
                     /locus_tag="ONT64_001348"
     CDS             complement(31486..32604)
                     /locus_tag="ONT64_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR2302772.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(32687..33520)
                     /locus_tag="ONT64_001349"
     CDS             complement(32687..33520)
                     /locus_tag="ONT64_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2302773.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(33513..34430)
                     /locus_tag="ONT64_001350"
     CDS             complement(33513..34430)
                     /locus_tag="ONT64_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2302774.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(34657..35781)
                     /locus_tag="ONT64_001351"
     CDS             complement(34657..35781)
                     /locus_tag="ONT64_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR2302775.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            35964..36872
                     /locus_tag="ONT64_001352"
     CDS             35964..36872
                     /locus_tag="ONT64_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2302776.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            37013..38626
                     /locus_tag="ONT64_001353"
     CDS             37013..38626
                     /locus_tag="ONT64_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR2302777.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT        11902 a         7752 c         6762 g        12761 t
ORIGIN      
        1 agaccgtgat atttctcaca gtcttttatc tactctattg ctatttgctc tcttagctag
       61 tactttaaaa gctaactcat tgaggtagtg aaaacgagag gtgtgttcag acagaaatcc
      121 aaaatcctca actgctagct gtggaatcgt agttttgtgt ttcacgatat ccgcaagcag
      181 gttcaaatct gataataaga attcttggct tatcagctgt ccgccaatta tttctaatgt
      241 atccggatgg tagatcagct ttgcatttac tgttttgttt tcttcatcgt attgtggaaa
      301 tagatcaaat tctcctgaac acgaaccggt attttgttcc aagaatggcg cttcatcttc
      361 tgtcagccct acagttccta aaaagtagtc tataatgact gctgctgtta tcttttgtct
      421 gaggtcgtaa gcggttggct gaccactcaa agttagcgct actgctcttg ccatatgtac
      481 agctcgagta actaaaggta aaaagagatg ccgtttattt ttgtcaaaac tgactgggac
      541 gatatctcca atcgcataga tgtccggatc acttgtctgc agatattcat tgactatcac
      601 agtctcatcc tcatacaaag ccactttttc ttttaacaat gtcgtattag gacgagtttg
      661 ggcagaaaaa atgaccatat ctgcctttat tgcttgagat agaagttgta cggatactac
      721 tttctcttct gattttttcc aatcgatcaa gaattcattt ggataaaaat tgatttgctt
      781 agtttgcagc tgattttgaa aactgttgat catatcctta tcgaaatatc ttgataaaac
      841 attcggcata taatcaacta gatgaataat gtatccttta tttctcaaag catctgctaa
      901 ttccaatcca atataaccag caccgaagat cactacttct tttgcttctt ttaaagcggc
      961 aagtacttgc ttagattgca ctagattttt ataggtgtag acattttcag cttcaatttg
     1021 tttatacaag gtgacaggag aggaacctgt tgctacgatt aatttatcat aggttatggt
     1081 ctcgtttttt ccttctttgg atgcgaggat gagctgatgt ttgtctggat caagttcagt
     1141 tactctccat tcggtgatca gttttgcccc actttcttga agagtccgca tgttatgggc
     1201 aacttcagct aaactacgta tctttccttt taaataaagg ttgattccat ttgaaacata
     1261 gcctagtcca tctttatgat cttcatcgat cagtaaaact tctgctttcg gttgaagttt
     1321 tcttagattt agggcagctg tgatgcctgc atgggaagca ccaatgatca cgattttcat
     1381 gttttattca tccttaaatt caatttttct aaataacatt cttttaaaaa ttggttcaaa
     1441 taggggcaat cataattata ttcgttgcta gtgaatacaa aaactttagc ggacttctgt
     1501 gacgggtact ctttcaattg gctagtcaaa atgatatcat actgcttttc tttctcgtat
     1561 tcgactaaat catatttgat cgtattcagc aattgttcct ggatgatttc tttcacgata
     1621 tccgccatta gcctttcata aggaagatca taagcaattt gcagacgtaa ttctaactgc
     1681 ttgatcagcg tccgcaaaat agatgcatag cgagcaaata attcaaagcg ggcattttcc
     1741 tcgtctactt cagtaaaatc aacaatagca aatgtttgtc tgaccaatct ttcacataaa
     1801 accaggaatt gtttatcttc tacttcagtt aaccgctgtt ttaatccttc ttggccaaaa
     1861 atattcatcc atcctctaaa ataggcaaaa tgagcgtgat tttgcatcac tgtcaacgca
     1921 atcttttgcg aaatctcttc tggaaaaaca taaaaagaga aatattcttc tatcgattgt
     1981 ttaaacgttc gattcaattg agctactacg tcgtctttgt atttttcgct tatcaaaaac
     2041 tcctggacat gcgagttatg tgtatctaat gtaaagttac tgatcatgaa aagataaaaa
     2101 accaacgttt cattttgatt ccaaggaaag gcatattgaa agaagtaacg ggtcaaaaat
     2161 gtatggaggt ccaataaaaa ttgtgtcgtt tgaaacaatc caacaggaag attgctataa
     2221 tctggtttgg attttttcgt acagcgcatt cgcttcttca taatggaaag ccatatttca
     2281 aatttcaatc gtccttcttc tgtaatagta aaactaaact ctttttgcag cagatctaaa
     2341 aaagtgtttc ctgataaatc actgtattct tttttgtttt caagataagg acgtccaaac
     2401 caaaataatt ggaaataaaa ataacaaatt tgactttctg gaccggtcag ccgcccgctt
     2461 tttattttca gatgaaactc atttaaagaa tcatttaatt ccttcactct gcgagacaaa
     2521 gtagccgaac tgatattaag ttccatgaca agtttctgcc gatcaaatga gccattttcc
     2581 aacagataaa gaagtatctg atattttgta gaatagcgca agtagcaatg ataataaaca
     2641 tcgactgctg ctatatttcc atgtctgatt aataaaatat tcccttcttg gataacaatt
     2701 tccaattgtt tgatattttc caagttagct tgtaaataaa acagatcttc tttcaaggta
     2761 gcccgatcaa ctgataagcg ggtaagtacg ttattaacgg aaatctctcc tttttcctct
     2821 aataaaagtt ggaaaagttt gatttgccgt tgttcatttt tttcaagcaa actgaaaagt
     2881 tccaagtcca ctcctccaag catttctgat tttaacagca gaagtttcct tctcatcctt
     2941 gaaaaagagg atctgtgaca gttatcacag atccatacaa attctaatca cttagctggt
     3001 tggcccagct gtcagttccc atgtgaccat gccttgatac gttgcgccag gttgagcatt
     3061 cgtactgcta tttacctcta agagtaaacc atcggtatcg ggaaagtcaa cgagaacctc
     3121 tcctttagct gttccatcac tctcataaat caacgtgttt tgaccttgct gaatcaattg
     3181 ttttgatcca gaatgattga agtataattt tgttccgctt aattcttgac cttgatcatt
     3241 cttaaataaa gaagtcaatg tccctgtcag ttgccaagga gacgtgcttg gaagccgagt
     3301 atcccgcaca tcaaaagaaa aatcgccttt tgcttcataa taacttgtct tcaacggttt
     3361 ggggagatcg ttaccaaatg taaaatcatc tggtacatta actaatgtta attcttgttt
     3421 ggggacaaga attgttttag cagatgtttg gacaactggg ttaggaagct tagcaccatc
     3481 tgctaatgta atattgctgg aaagtacagc tgcctttgtc agtatgctga cggattgtac
     3541 agaagtatct acagtattta attgatagtt gtaagtaaga tgaatggtat ctcctatttt
     3601 gctcaataga tctgctggga cagaaactcc ttgttgaatg ccggtatctg cgatagttgt
     3661 accatttaat tttaaggaac cattgatata agtagtattt tcaggaagtg agatcgtgat
     3721 ttttttgtcg gttatcgcat ccccagattt tgttaacgtg aatgtcacat tttcttgaat
     3781 cgtatctcct gatagagcag acaggggatc tcctgtctca ggttgtctgc taaggttttt
     3841 tagctgacta tctgcactca cagatggaaa attagccact gttatttgag gaagagtggt
     3901 gattccagct tgttcagact gccatttgta cgcaaaagac ggatacatgt tagtaagtac
     3961 atcgtccacc aagcgataat caaaataata ttgccctgaa gccaatgcag gtagtgtaga
     4021 agtagctgta tttacacccc cgttatttga ttggatcgtt tggaaaagtt gatctgtcca
     4081 tcctgtcgct aatttttggg ttcctttcga ataaagcctg taatggatcg aacccttttt
     4141 tagttcagtt gctgaccaat tggccgtgac ttggtacgat gcagaagaaa ggagctttcc
     4201 ccactgacta tcggctgtta agtttgttgg taaaaccgtt ccgtctagct tgtactgctc
     4261 caataccttt aaagcaacag gagaaagcaa tccgccttgc gcatctttcc cactgtacac
     4321 gatattggct gtctgattca atccacttgt gcttgctgga gtatgagtca gtattgctcc
     4381 ttgtgtaacg ttttttcctg taaaatagtt gactacgcga tcaaagcgac taaatggcaa
     4441 ctgatcaaaa cgtccttcag tcagccccat cgtcaatgtc cgactattgg tagagcctgg
     4501 aaggttctgg ttatttccat ccacgtattc tgtggtatca gcaaatgtat tatattttcc
     4561 tgttgaatca cttgttgaga tgtaactatt ccacgtattt atctctgcac ttgcagaaaa
     4621 tgcatatgct cctggattca agtttccgcc agttaattta ggataaaatc tacctgcagc
     4681 tggagctaaa atcgaattga acatacgatc ttggataata ttattcgaat cataaaaatc
     4741 aacagaaaaa acggcctttt tagacataga aggttgtaat ttacccttgg tcaaaatttt
     4801 gtttggactc gtatcatatt tatctccgtt ttctgtcatc ttcgcgacat tagctgtaac
     4861 atcaaattga agcgggatat cgtccacgtt gaattcgcct cgcgtaataa tagcaataat
     4921 atctgccaat agagtatttg tatcccacga ccccatccca aaacgcaagg tatggcttcc
     4981 tgctgataat aaactaccgc tagtaacctt gatgggggca ccggcaatat acaagtaggc
     5041 gttgttccag tcaattgctt ttaatacatc actgcttgat acatgttccg gcaaattgat
     5101 gatcgccaca aatgcaaatc cttgtgcagt gatattcacc aaatcaagta catttgttag
     5161 tttcacatgg ctttcccaat gaaaacggac cgtgccacct ttccaaggat catttgaacc
     5221 atcgttcaaa taagtagcag tcaatttagg caattggtta gccccagtta gttggacaag
     5281 cttcccgcca ttgactgcac tacgtccacc atttggaggt acgatatttt catacgtgta
     5341 tggatcataa gaaatggcag gaggcgttgc tccaacagct tctgccgaag gtgaaaagat
     5401 aaatatactt aaaacaaaaa gcgaagtaaa aaagaaggag cgaaaaaatg aaaatttatt
     5461 ttgtagcaac atgttcatcc tccttttcct ttcttctcca taacaacaac cacgcaagaa
     5521 gccataattc tataccaatg atcaaataaa gaaaacggat cttttcttca ttcgtctccg
     5581 ggaatacgcc atgttgatta gattctttat aaatcggtag ctgtgtctgc tgttttgatg
     5641 cgccattgac tgaaccactt ggatgcggaa ctggacttag atcataaaat gtaatcgtac
     5701 ctgaactttt ggtatctgct tggactggct cttgccagct gaagaacagc aaacttgcaa
     5761 taatccctcc caataagatc gatgttttca acgaaatcac ctcatcatgt tgtttctcct
     5821 tttttctttt tcttgtggac ccacaaaaac agcaaaatca accataacac tagaactgca
     5881 gcaaccaatc ctgcataaag ccatgggaaa gattgttctt gtactgcaaa aacggaattt
     5941 tcatttactt tttgtgcttt ttcttttttg atcgtaaagt tttgtgtaaa tcgccattca
     6001 ttactgtcgc ctgattttgc tatgatttct gcgcggtaat cgccggcttt tgctttatca
     6061 ccagctaaat caagattcaa gtgaaaattc gaatttggtg caactaaaaa ttcattagac
     6121 gtattttcaa tataaagctg attttcataa aagatcttgc tcgtaaatat caaattgctg
     6181 ataatcctag gctgaggatt ttggaaacga agctgaacag ttggctttgc gttgacctcg
     6241 tccaaatctc ctgataaaag tttcagatcc ggttctatcg tctcttgact ttcttgtaaa
     6301 acgactgcga gattataagc aatacgattt ttgatttgtg ctccttctgt agcattttct
     6361 gtttcagctt cttctttagg tgaaacatga atacccgcta atacacgtcc cttaaagctt
     6421 tttgctggga ttttcacttc tataggtata gttgtttctg catgtgctga tacattgatt
     6481 tcctttttcg caatgagcaa aacgtcacgg acatcaaacg gcaatgtctc atctaacgtt
     6541 gggttttttt gactataatc gatcgttcca ccatcactcg ttaccgccgg attgacagag
     6601 acctcaaatg ttcgttcttc atctgaagaa ttgctgataa ttacttctag tgtctgattt
     6661 tgttccggtg ccactaataa atcaaaataa ccgattgctt tatctatttg gttctctgga
     6721 aataccggtt tgattccaaa gtctcctgct gaatcctttg cttggctgat tgacggaaaa
     6781 atgaaaaata gacttaaaat agaaaaaaac gaaacaaaca gtcgtttact ttgattcatt
     6841 gtccaccaca tcctttcaac aacaactgcg gtaaaaaatc cttcacctat agtattttca
     6901 ccgcagtttg ttgaatttat ttcatactct atgctactgg gccagccacg aggttccaag
     6961 tcaaatcagc cgtgaatgtt ccagctgctg ctttcggcac tttcagtgtg atatttgctg
     7021 gatcataaac agaaagccat gttcccagac cttgatcttt tgctgctgta aatattggtg
     7081 tcgctgcact tgctttactc aacccagtta cagtttgact agttggtcca gtggattcgt
     7141 tatctgacgt acttttaaca gtggatgttc caaaatctaa ttcagcgcct tgcaaaactt
     7201 gtgaaccgtt tacaaaatct gaaaccgaaa ctgtcacgtt ccacccttgt gcttggccat
     7261 ctgcacctac acctcgacga tctgatactt ggagataagg tgtatcgttt tttgaagcgt
     7321 ctacttgata cgtttttgtt ccgctttcaa tctcgtgtgt accaaatggc aattcaggaa
     7381 ctacatccaa tgttaaagaa cctgtctggc cagttgctgg attttccgga tctgctggct
     7441 ctactggttt tgaaggatca gtagggttaa gaggctcaac ggtatttgtt ggttcagtaa
     7501 aggtaatatc tccttttgaa gtcgcttgac cctcagcaaa agctggtgtt gccaagccgc
     7561 ctagaagaag tgttgaaagt aataatgtcc caatcatgtt ctttttcata ataaatcact
     7621 cctttttctt agtgacctaa gtatagtaga gacagtatgc aaaaaatcct catgaattca
     7681 tccgcttact tgaaattatg accctttttg tataaaagag aaaaaaacaa taaaatcgtt
     7741 tacatgaata ttcttccttt tttattaagt atgaaaaaat atttcaattt tattttttcg
     7801 tttcaaatta ttctattagt gacatccttg aactataaaa ataggagtac ggcatcatct
     7861 gccgcactcc tatttttttc gtcatttatt tcttaaataa aacgattctc acgcaatttt
     7921 atttttttga tatcttctac tgtttgtgtt ccagctaatt gcatgaccat ttccaattct
     7981 gttttaaaga aatcaaacac ttgatggact cctgtacttc ctcctaaaga caatccataa
     8041 ataacgggtc tgccaatagc aacaagatcg gcacctgatg cgattgcttt gaaaacatgt
     8101 tgtccacgtc gtacaccgct gtcaaacacg attggtacac gtttatctac tgcttctgcc
     8161 acatattgca acgaatcaaa cgcagcaggc cccccatcta attgacggcc accatggtta
     8221 gatacccaaa ttccaccagc accagattct aatgatcgat agacatcttc ttctgattga
     8281 acacctttca cgtagactgg caaatcagaa tgagcagcga taaattctac atcttttgga
     8341 ctaagttttt gtttggaaga tttatataca gcatccatcg tttggcctac tcctgattga
     8401 taagcctgaa cgatcggcat tggcagcggg aaggtaaacc cgtttctgcg gtctgtttca
     8461 cggtttccgc caactgttgc atccgctgtc aatacgattg cttttgctcc attacgcttg
     8521 gccacttcaa gaatatccaa attgattcca ttatctttgc tcatataaaa ttgaaaccat
     8581 tgcggtgctt tctccccgcc tgcttctctt atctcttcta aagtacaaga agcgtaagaa
     8641 cttgctgtat agatcgtccc aaaatctgct acaccttttg cagaagcttt ctccgccttc
     8701 acatgagcta atccatgagc agcaactggt gccatgatga ttggtgcagt cagcatttct
     8761 tcgtcaaaat gtgtagttgt atcgggtaat tctacatctc ttaatacatg aggaataatc
     8821 aaacgatgat tgaatgcccg ttcattttct tgataagtaa ataagtcgcc agcaccactg
     8881 ctgatgtaac cataaccacc agctggaatc acttgtgaag ccgcgatttc taaatcttcc
     8941 atattgataa aatctactat tccttcattt gtacctgctt gatatgtttt ttccatctca
     9001 atgccttctc tctataaaat ttgaatgatg aacaatatga tagcgtttac actttttata
     9061 ttactcttcg acattgatac aggcaaataa tctgtttaca tttcagccat tccagatgtc
     9121 actactctgg aagaaatgat tgtaataaaa atagataaaa aaccgatggg actttgacaa
     9181 aagtagtgtc aaagtccctt tttttgaata aacggtgttc aaaagccatt ttctcggtat
     9241 taagtcaaat ttatgcaaaa atgtggaaag ccattttcac aaaatttttc ttactactcg
     9301 aaactaagcg actttttcac aacctctcga taaaacggtg ttcaatcagc tgaccctcgg
     9361 tattagctca aaaaatggca aaatatgagg aactattttg ccatttgtct ctctaatact
     9421 caggtcagaa cgttgacttc acagtctctt ccttaaacgg tgttcaaaaa cttgcatctg
     9481 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     9541 tacttgatgc agggcaggtt tttcacgacc tctgattcac ggtgtttctt tttcatcaac
     9601 atttttacga aatactgtgg aagactattg aaataagtgt ggattgtttc tttgtgtttc
     9661 aatcgtttct tgccattctt catttaattt tggattaccg ttttcttcct tgaactcata
     9721 aggtcctaat tcaaaaatcc cagacagtat ttgattcacg atttcatata gttcagtgtg
     9781 atcagacaca tgattcttga agatcatctc attatcacca cgtttgaacc tcacattcgg
     9841 gctctcatga tgaacctcta tgcatttttc tccttttttt aactgacgga cttctgaaaa
     9901 catctcataa atatcttctc cagtaaaacc ttcagcaagc gccagactgt ccacaagacc
     9961 gcctactgtc agttcagaga ttattgcttg attggaatct aattttaatg tatagcttgt
    10021 cgcttctact acgccacatg cattgcaacc gccttctgat agttcgcttt ttctaatcaa
    10081 ttgaacttct ttcattttct cttcctcctc ttaattttct acttatgtat ttttatcacc
    10141 tgacataggt gactcctatc aacgctatca tgtgcttttt tcctcagaaa tcctatttat
    10201 ccattccatc tatcccttat tcttctactt ttctttctct agcatataag agatacgcat
    10261 agtattcaag caaggattgt cgatataaaa agaacaaata ctgctatttt gatttctttt
    10321 ggctatatta catataaata aaatttatca tacctgctct ttttgactat atcatcatct
    10381 gacttctata cttcgtttgt attaaaactt aggaggaatc attcatgctt acagggttaa
    10441 acttattaaa taatcctttt ctaaataaag gaacagcttt tacaaaagaa gaacgggcaa
    10501 aatatggaat cactggtatg cttccaagta ctgtccaaac actggaacaa caatctgttc
    10561 aagcttatgg tcaatactta agcaaacaaa cagatttgga aaaacgtatc ttcttaatga
    10621 atctattcaa tacgaatcgg acattgtttt ataaattgat gggcgaacat ttagttgaat
    10681 tcatgcctgt cgtttatgat cctgtcgttg cggattcaat cgaacaatac aacgaaattt
    10741 tcttgaaacc gcaagatgct gcatttcttt caatcgacga accagaaaac atcaaagcaa
    10801 gtttgaagaa cgcggcagat ggacgagaca ttcgtttgat tgttgtgact gatgcagaag
    10861 gaatcttagg tatgggcgac tggggtgtga acggtgttga catcgctatc ggcaaattga
    10921 tggtctatac tgctgctgct ggtattaatc cagcacaagt tttgcctgta tcgatcgacg
    10981 ctggtactaa taatgaagaa ttattgaata atcccttata cttaggcaat cgtcatgctc
    11041 gtgtagaagg cgaaacttac tatgaattca tcgatcaatt cgttcaatca gctaccgaat
    11101 tattcccaga attgttattg cattgggaag attttggacg aggaaatgct gcagcgatct
    11161 tagaaaaata cgaagatcaa atcactactt tcaacgacga tatccaaggt acaggaattg
    11221 ttgtcctagc aggtgtatta ggcggattga atatatccgg tgaacaattg aaagatcaaa
    11281 taattttgac atttggtgct ggtactgctg gtgtgggtat cgcaaacatc cttttagatg
    11341 agatgatccg tcaaggtgta cctgaagaag aagcacgcca acatttctat caagtagata
    11401 aacaaggttt attgtttgaa gatacagaag gattgactcc tggtcaaatc ccattcgctc
    11461 gcaaacgctc agatttcact aatgctgatg aattgacaaa cttagaagca gttgtaaaag
    11521 cgatccatcc gacaatcatg attggtactt ctacccaacc tggagcattc actgaagaaa
    11581 tcgtgaaaga aatggctgct catacaccac gtccgatcat catgcctttg tctaatccaa
    11641 caaaattggc agaagcaaaa gcaaaagatt taatagaatg gacggacgga aaagcattag
    11701 ttggaacagg tatccctgct gctgatgttg aatataatgg tgtaacttac caaatcggtc
    11761 aagcaaataa tgctttgatg tacccaggtt taggacttgg tttgattgct tcaacagcta
    11821 cacgagtaaa tgctgaaatc atttctcaag ctagtcgagc attaggcgga atcgttgatg
    11881 taacaaaacc aggagcagct atcttgcctc cagtagcaaa aatcactgaa ttttctcaaa
    11941 cgattgctga aactgtcgca aaatcagttg tcgcacaaaa cttgaatcgt gaagaaatca
    12001 cggatatcaa agaagcagtt gaatctgcta aatgggttcc tgaatataaa tctttagaag
    12061 attaagatcg ttatagtaaa agaacataca gaaactttgt atgacgtcta aaaagaaaaa
    12121 agcgactgcg gcaaaacagc ggccagccaa ctcctgctct tttgtcgctt ttctttggct
    12181 tttttcatat gatacagaat aagaaaggat ctgtaaaaat ggcacaaaaa acaacaactg
    12241 caactgcagt tgaggaaaag aaaaaagaag gtttttggtc cacaaaaatc accggcgtaa
    12301 gtttgccact ttatttgatc atggtccttg tcctgatcat tgcaatggct ttagggaaat
    12361 taccgacgaa tatgattgga ccaatcgcag ttttggttat tttcggtaat ttatttcatt
    12421 ttatcgggac aaaaatccct atcgtcaaaa gttatttggg tggtggttcc gtttttgcga
    12481 tcttcgcttc tgctgcgatt gctacatttg ggatcttacc tgaatatgta gtgaaatcca
    12541 cagaaaactt tgtcagcaat atgggctttt gggactttta tattgctgcc ttgattactg
    12601 gtagcatcct tggtatgaac cgtaatctat tgatgaaagc ttctgttcgt tttattcccg
    12661 ttgcacttat ttcaatggtc gtctcctttt ttgccgtagg actcgtgggc atgcttatcg
    12721 gaaatggttt tgcaaattcc gttttataca tctcattacc tgcgatggct ggtggtgttg
    12781 gagccggagc cgttccttta tcaacgattt atgcaaatgt tttaggtacg gatgcttctt
    12841 ctatcatttc ccgcctgatt cctgcgacag caatgacaaa tgtcttagct atcatcggtg
    12901 cagcattagt tgctcgtgct ggacaatcaa tgccaaaatt caacggcaat ggtaaattaa
    12961 tggaaaaagg cgatcttggc gatggaaaac ctcgaactgt gaaaccttcc attccacagt
    13021 taggtgttgg tttgatggta tcattgactt tcttcatctt aggacaaatt ggtaactatt
    13081 ttgttcctaa agtccatgct tacgcattta tgattattat cgttgtcatc tgtaaaatcg
    13141 ccaacctatt accagagtat tatgaagatg cagctatcat gttcaataac ttgattgtca
    13201 aaaacttgac agcagctgtt cttgccggta tcggtattgc gttgctgaat ctgaatgtat
    13261 tagcttctgc tttgacatgg caatttgttg tcttatgtct gacaagtgtt atcgcgattt
    13321 cagttgtttc aggatttgtc ggtcgtttgt ttggtttata tccaattgaa tcaactatta
    13381 ctgctggctt atgcaacaac tcgatgggcg gaacaggaaa tgtagccgtc ttatctgcag
    13441 ctaatcggat ggaacttatt gcttttgctc aaatgggcaa tcgacttggt ggtgccatcg
    13501 ttttgatcat ctctggtttc ttgatgcaat tactttctta gtcaaaaaaa caaaaatacc
    13561 tgatcatcaa gtatcggctt ggtgatcggg tatttttttg ataaagattt acaggatttt
    13621 caaaaatctt ttttgcattt tatcggaatg ataaccgatg tgttcataaa attggtgtgc
    13681 ttctactctg tattctgcag aattaagcct tattgtcgtt attcctcgtt ttttcgcttc
    13741 ttcttctacg ccagtcatca aagcctttcc gatgcctctt ttttcagcat atttagacac
    13801 agcaagtgcc ataatattga acattggatc gctaaaaatc gattcatagg actccgcttg
    13861 tacatagcca gctgcttttt tagaagtagg atcttcataa accaagaaaa aatttttctc
    13921 tggctccttc agcagtttct tcatttgtct caccgtaacg gttaatggta tatcataccc
    13981 taattgatcc gcattgattt cacgtagtgc ttctgcatct tctaaacgca tttcccgtaa
    14041 catccaaatc accagccaat ttctgatttt ttaaaatgaa tttttttatc atagtatact
    14101 ctttttttca tttagaacaa tatgtttcag tgattttgac ttgcgttagt ctagattctc
    14161 ctaatgtttg taagatgaca aaggcattgt aacaattgac caaaagaacc acgaggacaa
    14221 ttacttgaaa aataaaggtc actttttttg gagacaaaac cctgctaaat cttgctcccc
    14281 acaaacctcc aataacagca gcggggataa cataaaacaa catagataaa tcaaagcgca
    14341 taaagctact tgttaaagca atcgttacca attttgccag ttgagaaaag aaaatcgtag
    14401 atagagaata tagtgttgct tcttttattg gcaatgcaaa catcagcatc aacaaagaaa
    14461 cattgactgg tccaccacca atacctaaaa aactagctaa aaagcccaga acccccccca
    14521 catataaggt accaagtcca tgacgtcaaa tgaaattttg gctgatgatg cttggtgtag
    14581 aagaacgcaa aaaccaaggt caataccgtc aaaaaaattt ggatcagctg cacatgcttc
    14641 tcgttttcaa ataactgtaa aaaaacttcg aatacaatgt tgccgactac tcctccaaca
    14701 acagctccac tagataccca accgacaatc tgccaattca gtgatttccc gcttgatact
    14761 tgtcgcacag tcgaaacaat ggacatcgtg aataccgcaa ctgttgagta aaaagaaatc
    14821 cctgcaacag aatgggcgcc gataagatca agaatcggtt tgatcaacac gcctccaccc
    14881 atcccggaaa tagcgccaac tgtgttcgcc agaacgatca ccacaaaata aatcagacca
    14941 atcatcttgt atttcctttc tcttgtttat atcttttagc gattgttact cttttctttt
    15001 attatacagt aaaagaaaca aacgaggctt cgaaagcttt tctacttttt aaggaaattt
    15061 agtgctaaac gaaaagaggt tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca
    15121 aacaaaaata gctcctcata attttcgttt ggttgaactt attaccgcag atgcaagctt
    15181 ttgaacaccg tttaagaaag aggtcgtgag cctgccgttc agctccgagt cacaaggaac
    15241 aatttcaaaa aacagcttct catgtttgtt gaatattgtg acttgtgacc gaggagttgg
    15301 caggagaaca ccgtttaagg aagagactgt gaagtcaacg ttttgacctg attattagag
    15361 aaacaaatgg caaaatagtt cttcatattt cgccgttttt cgagcgaatg ccgaggggca
    15421 gctgattgaa catcgtttat ttggaaaaag ggattgtgac aagacgtttg tcacaatccc
    15481 tttttttata tcttattaat tcattctttt gttctattct tcttcttccg ccatgaatgt
    15541 attgaatact tcttcaatca tgtcccattc tgcgtctgtt tcgatttgtt ctaactcgcc
    15601 ttctgttcca tcttcgtttt cgatgtatgc ataagcttgt aattctacat cttcatcttc
    15661 tgaaacacca gcaggatata aaagaacata gttacgtcca aattcttctt gtccgtcaac
    15721 tgttagtagg atttcatata atgtttcatt tccttggtca tccaccaatg tgatgtgttc
    15781 gtgtccttca tggtcgtggg aatggttatg ttctgtcata agattacctc ttttctttta
    15841 ttattaaaat aattgcttac ctgagccgtc taaataattt tgtaagatca tcacagctgc
    15901 taatttatcg atgacttttt ttcgtttggc tcttgaagta tttgcctgct ctactagcat
    15961 acgttctgcc tgaacagtgg ttaatcgttc atcttgataa tcaactggca aggagaatag
    16021 ttctttgatc ttttctccgt atgacatgga agcttctgcc cttggtccga tggaattgtt
    16081 catattttta ggtaatccaa ctacaaaccg gtctacttca tattgtgcta ctaattcctt
    16141 caaccgatca aagccaaact cttcttctgc ttcattgatc cgaatgatct ctactccttg
    16201 agctgtccag ccaaaggggt cactgatagc aacacctact gtcttagaac cgacatccaa
    16261 gcccattact ctcataaatt cactccatga tttgagaggt agtccttgac aattacttcc
    16321 atgatctcat cgcgttcgtg acggcgaatc aaatttcgag catcacgata acgaggaatg
    16381 taggccgggt ctccagaaag caagtagccg acaatctgat tgattggatt gtagcctttt
    16441 tcttccaaag cccgataaac aatcgctaat gtttcgctca cttctttttt gcgactatcg
    16501 tcaaaaccaa aacgtacggt ttcatctgta aaacccatga ttctacacct ctttcccgca
    16561 aggtaattcc agcttacctt ctacaacctt attttactcg aagaaaggga aaactacaaa
    16621 caaatattcc aaacttccga ataaattaag aaacactatt tgtatgtttt tttccttttt
    16681 tctgtagctt tatcaaaagt actcttttca taaagtatac gctatatttc tttgaatgta
    16741 tgtttttaca caattttttc ataatattca taaaaaattc gaatagctgc tactaatttt
    16801 ttgtttttgt tgcttgtcct gtctcataat tgatcgtgta gccatcttga acattgtaga
    16861 taaagacatt gaattcaatt tttttgtctt caatgctttc tgcttccaat tgcactcccc
    16921 tggctaccag ttcattcccc tcaaaatgag gagtgacacg gtagcgtaca tgatgatttg
    16981 tttctttgat atatgccgca atatcatttt cgtgagcaag catgtatgga gcattcaacg
    17041 aacgtgtccc tgtcatcaag tttttaatgt tgttattttg accagtcagc tggaaaccaa
    17101 ttaaatggct gcgattatat aaccattgcc cgtcacttaa tttcttttgt ttccaacctg
    17161 tcgggtcaat atacaaaggc tctctctttt cagtagggaa caaatctttt ccaagcatag
    17221 cattggcttg tcctactctg ttcaatttat caatatttga atagctttcc catgttcctt
    17281 tggaaaggct aagatcctct tttgtaaatt ccggctgatt cccattgatt tctatttctt
    17341 gatgttttcc atcatattct ggcacatccg ctacttgaga agcttgtggt gtggaatcaa
    17401 aaatagttgt aatctgctga aagatatctg aatctttttg tgtggtggaa acaccgaatg
    17461 cccctgcaat cacagcagcg acgatcccca cgatcgcctt ggttgttttt ttgctcattt
    17521 ctttactcct ttactaacag ctccctaaat cttaacatat gttcccctat aaataaatac
    17581 tagaaagaaa tagaaacagc aaaaaataaa acctgtttta agatggctta aaaatacaaa
    17641 aaagagacgg attcctcgtc tctaatcagt caccactcta tctgtaaggt atttattatt
    17701 attcaaaaat tttcttcata ctcaagacaa tacactgatt tcacaaccgc ttggtaaacg
    17761 gtgcgcaaaa gcttgcatct gcggtaataa gtccatccaa gttaaaaaca taagaagttg
    17821 tttttgcttg gctgttctta ttacttgttg cagagcagct ttttcacaac ctattttaag
    17881 cgggtgacgg gaatcgaacc cgcgacatca gcttgggaag ctgggatttt accactaaac
    17941 tacacccgca atacaaaaac agtatacgcc tcttttattc cttcctcaag tttttttatt
    18001 ctttacgctt ctggtatgtt ttgtagtaaa ataaaactaa tcaatcaatt attggagaag
    18061 atgctatgac gggaaaagaa ttagatctgt tgctggacac ttgtttactc gctgggaaaa
    18121 tcatgatgga aagcaatgcg gagatgtatc gggtagaaga tacgatgagt cgaattgctt
    18181 tagcttctgg aaatttccgt ttggtcagct atgtcactca aaccggtctc tttattggtc
    18241 ttgatcgtac ctcgaccatc cgaatggaac aaattaccaa ccgttcgatt aatttagaaa
    18301 aagtagtaaa agtaaatgat ctttcacgta aatatgttgc ttcagagcta acattagaag
    18361 aactttactg cgaactgcaa gcaatcgacc gagaccgttc tttctttcct gtctggttgc
    18421 aaatcgttag cgcggcagtg atcagtggaa caattatggt gttatttggc ggaaagatga
    18481 ccgatttacc tgtgacttta gtcattggag gattgggata cgcagcttat ctatatagtt
    18541 tgaaattttt tagaatcaaa tttttatcgg aatttttatc ttctttattg attggcatag
    18601 ccgctatcta cagcgtacgt tttggcttag gaagcaatca ggatctaatc attatcggat
    18661 gtgtgatgcc tttagttcct ggagttcaaa ttacgaacgc tatccgtgat ttgctagctg
    18721 gacattatgt ctcaggagtt tctcgcggaa cagaagcgat gatgactgct acgatgatcg
    18781 gttttgcgat tgcttttatt ttccagcttt tttattagga aaggaacaca agtatgggga
    18841 cgatatttat tcagttttct tttagttttt tagcgactgc ggcatttgcc gttattacaa
    18901 atgtacctag gcgttcattg atttgttgtg gtctttctgg tgcttttggc tggatgatct
    18961 actggatctg tgttcaaata ggcggatctg ctgcttttgg ttcattgctt ggttctttag
    19021 gagtagcatt catcagcgat cttttctcta aacgtttgaa aatgcccgta acgattttca
    19081 atattcccgg gatggttcct ctagtccctg gtggtttagc ttatcaagcg gttcgaaatc
    19141 tcgttactga atcctatcga gaagcggctc attatgcggt ccaagcaatt atgattgctg
    19201 gagcaatcgc tttaggtctt gtattatcag aagtctttaa ccataacatc cgtaatttca
    19261 aagtaaagcg tgacgcaatc atgatgaaac gaaaaaaacg gctttttaaa taaacgaagt
    19321 gtaacaaaga aaaggttgtg aaagagacgt tcagccttga gcgatgacaa aatatctcac
    19381 aaacattgcg acaagtaacc gcaggagcaa taagaagtaa ttatgagtat taaaaaaagt
    19441 tttgtaaaaa tagcttctca catttttaca taaaattgac ttaatactga gaaactgact
    19501 tttgaacacc ctctatctgt aaaaaagggg ctgcgacatg atttttgtca cggcccctct
    19561 tctttattca aattttttcc aaaacgttcg ttgataataa acgatccata gagcggaaag
    19621 tattagaact aaaccgatca caggaatatt cactaaccaa aatggataaa acaaagccaa
    19681 cattccgtat aagacgataa gaataatact gatgatcaat gaacccatca tagttgcgac
    19741 taaacccaaa cttcctgaac ctctgttaaa taattggtta atattggtcc agttcagcag
    19801 cagcaaccga taatctctag cgaaataacg tagtgacaat aagtagcagc cgaataaaga
    19861 accaaatagc aagctaggga taaaaaacaa aggcagctga aaagacagtc cgcttaataa
    19921 tgcgatccca ccagttaaaa aactctgcaa tatccatccg aatctgaact tttcttttaa
    19981 atattggttc atagagattg gcaaagaacg gataaacaga aaattttctt gatccaaaga
    20041 aataagattg ctgataaaag aagtctgatt aactgttaag aaagccaatg cgatccctac
    20101 caggaaaaca acaccgatta acttattatc taaatcagtt aattttattt cgcctcctag
    20161 ggcaaaagca agaataaaaa tgattggagt taacaaggaa gaagaaagaa cttgcataat
    20221 caaattaggt tcttttacta attgagcatt gtaactaaat aaaagctgat gcacattttg
    20281 gttcttttta tgggttcttt ttattttgaa ctcactgttt gaatcagtca gttgctctgt
    20341 aagctttggt actatcagct gttttacagc aaggaaaaat cccagagccg tgatagctag
    20401 tattccccaa cccaataaac cttgttgaga aagtggctga ctggcagcat catagaatgg
    20461 caataatatc gaaatcggat gccgatctgt taactcattt tcaatagtcc ccgtttgatg
    20521 attcatccac atgattccag caaatgcgat cagcatcgag ctgccaagca agatactaga
    20581 aactgttttt ctatgttttt taaacactgc tgttcgagcc aaaccaaaaa caatcaaact
    20641 agccagacaa aaaagaataa tcaaaaatag agaaaacaaa aggattgaga taatgatagt
    20701 aagcaaaatc cctagatcag ctcgccatcc ggttaaaata aacatgacta gaagcggaaa
    20761 aacaaaagga atgatggtca aagcaactac caaaatcttt gctgaaaaaa cctgttcttg
    20821 tccaaaaggc agaggcaaaa atacttgcag gtcatttcct tcaaaaaaga tattataaat
    20881 gactgatata ctttgagtta aacctaacat accaaaaagt gccacgtaat aagtaaagaa
    20941 acctggcaat ttaacaaagt tcatagcaaa catagcaaat ccgtaaatca gtaaaaaaat
    21001 cagccctgaa aaaagatttt gagacaaaag atagatagtt aatcgctttc ccttttttcc
    21061 tttctctctt gctttattcg tgagctgtgg attaacatat aaaagattaa ctcgaattaa
    21121 ttctaataat tgtcgcttat acatacggtc catctcctgt cgcttgcctt ccagccattt
    21181 tcaaataaat cttttctagc gtttcatcag gtgagccatt caataattca ctaactgccc
    21241 cctgataaat caattcacct ttcttcaaaa tagccagttt atcgcataac tgttgtgcgg
    21301 tatccaatac atgtgtagaa aaaatgactg tttttccttt tgcggcatga gccttcatca
    21361 tttctttcaa atcaaatgct gcttgaggat caagtccttg caaaggttca tccaataccc
    21421 aaatatctgg atctggcagc aacgtgccga tcaaaatcgt tttttgacgc attccatgtg
    21481 aaaaacttgc gattgtttca ttttgatgac tatacatatc aaaaagcgct gtcaattctg
    21541 ctaaacgctt ttcttttttt tgtgtgtcta attcataggc agctgaaatc aagtcccaat
    21601 attctccagc cgtcaattgt aaaaaaatat ctggggaatc aggcacataa ccgatttttt
    21661 gtttgatact caatcgattt tccgtcaatt tcattccatc tactaaaatc gttcccgatg
    21721 atggttcaat aatactaact aaacttttaa tcgtagttga ttttccggca ccattatgtc
    21781 ctaaaaatcc aaaaatttcc ccttgtctta tcgagagaga caagtctttt aatgcttgtt
    21841 ttttcccgta acttttactt acattgatga attcaatcat ttctgtactc cttttgtttg
    21901 ttattacttt ttattgtacc cttcgttatt ggtggagtaa atgtcaaaaa gagtgcataa
    21961 caaaaaagga tagaaacgac taggctgcta cttgggttgt tctcttctac aggaaaacac
    22021 tcttctaaca ggtaagatat gagtctttgt tttctttaaa atgattcatt ttctatcttt
    22081 tcttgcataa aattctttag tttatcaata cttctctttc ccatctctag ttgttctcca
    22141 tttgcaaatg ttactatttc gtttactctg ctaatagatg taatgttttc agtattaatg
    22201 atataagctt ttaaatcttt acataaatac tcatattcga gtgatttttt atatttttta
    22261 attgtgccgt ttacgagaat ctcttcatct ttcaatacta ggcctatttt atttttataa
    22321 cctggcatgc tcattagata aaggatttca tcgtaatgga tatagtgatc gctattacca
    22381 taacggacag ttgtccacct ttttttcgta gcatgttgaa caggatagtt ttctttttct
    22441 ttcaataagt aactttggat gcttttttta tttttttcat aagtgaattc ttttatcaaa
    22501 taaccactag gcgtaatctt tcgattaata acagttattg ctttattcgg atgagacgtt
    22561 agataaacaa ttttacactt ttcattcttt tctctaagca tttcgcctag ttcgataccg
    22621 ctataatagg cgttcaaatc gatgtctagg aaaaaaatat ctgaggaata gatttcatta
    22681 tttttaatga attcaacaaa ttgtataacg ttcaatccct gaacgatctc aaatttccaa
    22741 ggacttaata aaattctgga ttcttccaaa atttctacta gcattttttc aaaaaccaaa
    22801 ctatcatcca aaataaatat acgcgttacc ataaaaagta cctccactcg atattttact
    22861 atcaaaatca tttcatacaa cttttcaaca tacatgttag gaataaattg taattctctt
    22921 cacaatcttt tccaacacct ttgaaaccga tatcaaatac tttcaggatg ctgcttctaa
    22981 aataaatatc aaacaaatga atacatcatg caaaaaaagc cagacaaccg acgtccttgc
    23041 gtgtcagctg tctgagctat gtttatttat tttgttcttt taaaaagaac gatacacata
    23101 ctgatttttc ggagcagaga ctttctcagc gtttgtaaca actacagatg ggatttcacg
    23161 cttgaatggt tgataatagt ctgattggat ggttccttct attttatacc attcatcatt
    23221 tttaaccgtt actccttccg gcatatgcgt caacaaacca aagactcctg aatctgccac
    23281 acaatggata atgccgaatc ggaacaagaa gatatctgta ttgctatcgt tcggtgtttg
    23341 gtaaacaaat ccttcatagc tgattttttt gccaatgaat tcgctagggt aattataaat
    23401 tagctccata acttctaaat agttttcatc tgtgatcttg atagtttgct tatccccata
    23461 cttttctttc aactcattca tttggtctaa gtagtctgat ttgttgaaat aaatactggt
    23521 atccggcttt aagtattgtg tctgcatctc cggatcccct actgattctt tacttactgg
    23581 gaaattaaat cctttggctt ctacgatcgt agtgtcgaga ctaactgttg gaaacaacgt
    23641 ccccacgatt actggcaaag ctagcagtac ataagcgatt ccacgttgat aaggttttga
    23701 caacccatgg tcatgatcgt gctcgtgtgc cttttcttct gcttttccgt ttttgaccca
    23761 caaatacagc tgaactaaag ccaaaagagc agataaaatc attgaaagaa tagctaagta
    23821 gcgataatgg acattgatat attgatcgag tttgccagaa acttgcaagt acatcatcaa
    23881 ttcgaaatac ccagataaaa tcaaaaaccg cagcatattt tccctcctaa ataaataatg
    23941 tgaatccaat aacgctaagg gcaatcagtc cgatcagcga taaaataaag cgtcctgtaa
    24001 aatatcgttt catcatcagt agatttttga tatcgaccat tgggccaaag accaaaaagg
    24061 cagctacagg tgctgtgccg aacagactta acaaagaaga tcctataaac gcatctgctt
    24121 ctgagcaaag agacatcgta aaggccaata acagcatgat gataatggct aaaatctttg
    24181 ttgagccgag cgttagcata attctagttg gtaaataagt ctgcatcgca gctgcgatta
    24241 atccgccaat gatcaaataa cgacctgtat caaaaaattc gtcgattgaa tgtgtcaaca
    24301 catgcccagt ttgatggatc agctgatttt ttggttcatt cttctcactt tgttcttctt
    24361 catggacatg atgatggtga ttttctgcta ctttagacgt gattttttct ttcaatacag
    24421 cgtctttttg aaaataggca agccagacac caatcacaag ggcaacaaaa aagctgccta
    24481 tcacccgcca gcttgccatc tgccaagaat tgccaaatgc gataaaagtg gaaaaaatca
    24541 cgattggatt gatgatcgga gcagtcagca taaaagcaaa cgctgtgtga actggtacgc
    24601 cttttttaac aaattgatga acgatcggta cgatgccgca ttcacatgaa ggaaagaaaa
    24661 atcctaagat cgaaccagta aaaatagaga agatacgatt ttcaggaagc catctcttca
    24721 cacgttctgg ggtcaaaaat acttgtaagg cgcctgagat aagacagcct aataatacga
    24781 aaggtaatgc ttcaataacg atcgataaga aaatcgtact catctgcaaa accgaatttg
    24841 gaataaaaga aaacatgagt ttcctccttt ttatttctag ttcattcgct ttgcaaatat
    24901 acgcttttat tcaaaaaaaa caagttgctc tgtaaatttt tacacaactc ttatttttta
    24961 ccaaagcact tcaattgact ttttctgtta ttcccattaa attaaggtct catacatatc
    25021 acttctatca taaaagggag aataaaaatg aaacaaacca ctaacttgac tgaagttcaa
    25081 gacatactgc aacagtcatc cgttgccgtc atttatcttt cgatgcctaa ttgcagtgtt
    25141 tgtcacgctg ttcgtccacg actagaggaa cttctcacac attatgatat tccggcactt
    25201 catttggatg catttgaaac accagaagta gctagtaaat ttgaagtgct gactgcgcca
    25261 gttgtcctga tttttcatca aggaaaagaa gtctttcggc aagcacggtt catcgacttt
    25321 aaaaaaatcc gttatctact agatgaactg acagcagaag atgattccct ttcttatgaa
    25381 gaaatcttcc gaacaaaata atcgttgtcc aaacagaaaa aagcgattga acaagtgaaa
    25441 gttttgcctt catttgttca atcgcttttt atttttattc tgctgtattt tcggaatgcg
    25501 catttgcgtc agaatgcaag aaatagatca aggtttgcaa ttcattcgtc aaatcaacat
    25561 tttggatcaa tacatctgac ggagcattca atcgagcagc ggtaaagttc aagattcctt
    25621 tgattcctgc ttccgttaac tgattgacta cttcttgtgc tctttcagcg ggtatcgtta
    25681 aaattgctac ttcgatttgt tggatcttga tttgttctga catatcttcc attggataaa
    25741 cggggatacc gtctacgatg cgcccaacga tttcctcatt cacatcaaaa gcacaactga
    25801 tacgtatgct attactttga tggaatttgt attttagtaa agcacttccc aagttcccta
    25861 caccgatcag cgcaacattt gtcaattgat cgtcgtttaa tgtctttgca aaaaagtgca
    25921 taagatcttc tacatcataa ccatacccac gttttcctaa ttcgccaaaa taagaaaaat
    25981 ctcgacggat cgttgcacta tctacttgaa cggcttcact taattcagtt gaagacactt
    26041 ttttctttcc agcattattt aaaatcctta aatagcgata atacaaaggc agtcttttag
    26101 ctgtcgcctt tggaataata tgatctttca cgaaatgacc tccacgtatt gtctaattac
    26161 atctttgatt tctccatcat agcagtattt ataggaaaaa atcaattcca ttgctcagat
    26221 attgtgaaat tttattcttt attcatttat ttatcatttc tgcaaatctc gcagtataat
    26281 ggaatttatg gtagactact aattgaaaaa tagtttttta tttgaggaga attcacaaag
    26341 tagaaaagag gattccatga tattattaca agccaatcag attgctcgcc attttggtgc
    26401 agaagtctta tttgaaaaca tacatttaga aatcagtaca ggcgctcgtg tcgctttagt
    26461 tggcagaaat ggcgctggaa aatcgacttt attaaagatc attgctggaa ttgaagcacc
    26521 tgatgaagga aagatcgcta aaaataaaac agcgactctc ggatacctcg ctcaggacac
    26581 aggacttgat tccaatgaaa cagtttggaa cgagatgctg aaagcttttg aacaagttag
    26641 acaaatggaa gagcggatgc gcgagctaga gattttcatt agcgaaggta cgccagacac
    26701 acctgcttat agttcttatt taaaagaata cgaccggctg caacacgatt tttcagatcg
    26761 aaacggctat ggttatgaaa atgaaattcg ttccgtattg catgggttta aattcgattc
    26821 ttcattttat gatcagaaaa tcgatacttt atcaggcgga cagcgcacac gtttagcttt
    26881 agctcgcatg cttttgcaac accctgacat tttgattcta gatgaaccaa cgaaccattt
    26941 agatatcgag acactttcct ggcttgagaa ttatctgcaa agctatactg gtgcattatt
    27001 gattgtttcc catgaccgtt attttttaga taaagtagtc aatgaagtct atgaattaag
    27061 cagaagaaaa atgcgccatt acaaaggtaa ctattcgaaa tatctagatt tgaaagcaga
    27121 acagttagca agtgaatgga aggcttatga aaagcagcaa acagaaatcc ataaattaga
    27181 agacttcgtt gctcggaacc ttgttcgcgc ctctaccaca aaacgagcgc aaagccgcag
    27241 aaagacatta gaaaaaatgg accggctgga tcgtcctcaa ggggatgaaa aacaagcaaa
    27301 ctttctattc agtatagaaa agccttctgg aaatgtagtc ttgcaaatcg aagatgccgc
    27361 tatcggatat gataatgtca ccttatctga accaatcaat ctagatatcc gtcgtcaaga
    27421 agcgattgct ctagtaggtc cgaatggtat cgggaaatcg acgttgctga aatcaatcat
    27481 tcaccagatc ccttttatcc gtggaaaaga aacgctaggg acaaatgtca ccatcggcta
    27541 ttacgatcaa ggtcaagctg atctgcatac aaacaagacg atcctagcag aattgtggga
    27601 tgagcatcct accactccag aaaaagagat ccgaagtgct ttaggtgctt tcttattttc
    27661 tggcgaagat gtagaaaaac caatccctct gctaagtggc ggtgaaaaag cacgggttgc
    27721 tttagccaag ttagcgatga acaaagaaaa ttttttgatt ttggatgaac caacaaacca
    27781 tttagatatt gacaacaaag aagtattaga aaatgcattg atcgattatg acggcacact
    27841 gctttttgtt tcccatgacc gatatttcat caatcggatc gcgacaaaag tcgtcgaact
    27901 ctctgaaaat ggcagcaaag tctatctagg agactatgat tattatttag agaaaaagca
    27961 ggaagaagaa gaaatcgccg aattgttggc acaagatgag ccagaatctg ctcctgctcc
    28021 cacgaacaaa tctgattatt accaaagcaa agaacagcaa aagcttatcc gtagccttca
    28081 acgaaaaatt actcaggttg aagaagaaat ggcgcggatc gatgaatgga tcgatcagtt
    28141 ggaacaagaa atggtcgcgc cagaaaacct ggaagatcac gtaaaactca atgagctgaa
    28201 ccaagattta gaagctgcta gacaagatca agaaacaaaa ttgacggaat gggaagaact
    28261 aagcttggaa ttagaagaat atgaaaatta acgaaaaaac caaaacagaa accattcatg
    28321 ctgttttggt ttttcttttt ttattttatc aaacaaaaat tccatattta cctttttcac
    28381 caataagaag accaattact agtcattctc actaaagctt ctaaataata gtaatcaccc
    28441 caaatacaag gtgtatttac cccattattt ttgggcttat tataaacaga ctgtgttaaa
    28501 aagccatctt cttcttccgt tgtagcatat ctttctttca attgtcgcat aattttccaa
    28561 acagcagact ggtataagtt cttttctgtt ttgtctaata gacctgattc tattaattct
    28621 aataatccac aaaccatgat caccatacta gatgtatctt tttcttctct gctagaatca
    28681 tcaaagatca aatcccaata acaaatggta tcttctggca accgattcat aaaataattg
    28741 gctaatattt tgcttaaatc aaaatatttt tgttctttca aataacgata acttaatgca
    28801 aaaccgtaaa tcccccatgc ttggcctcta gcccaacagg aattatcact ataaccttga
    28861 tgcgtacttc catatcttgg ttttccatct ttgacatcca tataaaatgt atgataagtt
    28921 gaaccatctt ctcttactaa ataatctgct gcattttgaa tatgagcctg agcagctttt
    28981 ttatattttt gatttccagt ttcttcactc gcccaataca ataatggaag attcatgcaa
    29041 caatccatga tcattctacc ttgttgtgaa ggatcactag gatctcccca agcttgtata
    29101 actcctgcct tctcccaaaa acgctgaagt aatgtttctg cagcatcaat tgaagcttcc
    29161 ttcatttttc gatttcccgt tattttataa tctgctacgg ttgataaaga atacaaaaaa
    29221 ccaagatcgt gatgattagt gataatttga cagtcaatcc tgtggataaa atctatatct
    29281 gctttttcag ctgtttgttg aaataaagga tcacccgtct tctcaaataa caaccaatta
    29341 attcctgtcc aaaatccttc tgtccaacca atattgtctc ttaatggata acgtcctttt
    29401 ataggacact cgcttgggaa ttgatactga ctgttactga tacgtttgat taacctctcc
    29461 gtgtgtctgc gacaacgctc gatctcatta ttccattctt tttccattaa ttctttataa
    29521 cattcattta tccatttttc tggatgtatt atctcttcaa acatcacttt atcaagcatt
    29581 tccccaccag cctttagcat aattttagtc tttttttatt gtttttattt tggatagtta
    29641 gcccggttgt cccatcaaaa tcaattttgg gacgattgtt ccatacttct aaactataat
    29701 cccgatcttg atgagcaacc actcctgttc cgagccaatg ggttcctttt ccaaatgtac
    29761 cctctaatcc aggaataaaa gtgttttccc atgtcatcaa gtttgtattg ggcaaaccca
    29821 taatagctgt ggattttcgt ttaatagtat cgtcacttaa acagataata cctgaaaagc
    29881 cagcttcact caccctatag ttttccttgt cttcttctat gattattcct tctttctcag
    29941 gagaacattc aatagcaaac cctgtctcat atagaacata ttctgtatct aattctactt
    30001 tgtgtacgcg aatctgccag ccttcaaaag gaataagcca agttctgatt tttgcctgta
    30061 aagtaggttc ccatgtagag gcaagtacct ctttcttaca tacaatgtct cgtacattat
    30121 ttcgaacgat gaactgtcca taggttttga tgctctcatt cttaaaactg gtcgtgatat
    30181 atgtgtccgc tcgctgaatc cctagcattg aatccattgc catttctgct ttcaattgat
    30241 tcgctcgcgg tatcgaaaaa ccaaattgac tagaataagc aaattttaaa tatttttcat
    30301 tggtcaatgt atggaaattt ggccctaatt gtcccgcatt caaaaaaaat gtatgtccat
    30361 tatcatgttg tatgagcaaa ttggcttctg aaagaagttt agttccttca cgaataggca
    30421 taggcatttc tttagccgtc caatactcat ggtcttcagg tagcgccaac aataaaaaaa
    30481 tcttattcag ccataatgga gataacgtac tattataggg ttcagtcaat gtcaattgag
    30541 ggtaggtata accaatattc aggatacctt tttcatcaaa tatttctttt tgcatccacc
    30601 atcttatatt ccgattgact aaccccttga tttcacctaa tttaaaaggc aaaagatcgg
    30661 cccacaacat agctgaccaa aagcttgaaa ctgcaaaacg ataaatcatg cttcgaccaa
    30721 aaggcacatt tgcaccatct tccgcaaaaa aatgaatata gtctctagaa aaatcaatag
    30781 ctcggttctt atatatctgg ctaatgggct tttctggttc tagtacgcta taaatcaaac
    30841 cataataatg aaatgcaaaa ggattataat aatcttggcg acctcttgaa ctgtctcgat
    30901 accagccatt ttgaagataa caattttcta tattatcaaa tgcttctttc agtgctgatt
    30961 ctttaactgg ataaccaaat ttttggataa ttgttccgac aattatctta aaaaattgcc
    31021 aattaccatc tgcacattca tattcagaaa tttgcgagaa ccaatgaata atctggcttt
    31081 tttctcctgt atctaatttt ttccataata attgttcatg atgatataac gctaataaca
    31141 ttgcgggcat ttctaccata cgctggtctc tttcatgtat ttctccccaa taatcgggat
    31201 gggaaggatc tgttccagct tttattcctg atagaattaa tttcaaccat cgttcatcta
    31261 attggtcaat tgcgggcccc gctccccata aaacccgaga gaatccttcc attcctgcaa
    31321 ttttatttcc atatcctacc ccttgatgta tataatcaat ttgtgcgcca cttgctcgat
    31381 aaaacggctg gagtggttga tttatctttt taaataagtc aaagtaatcc tgtctggtcc
    31441 ttaatagtgg tactggatgt ttcattgtat tttctcctct ttttactacc aaatagctaa
    31501 ttccgactgt tctaatcgaa gtaagccttc aataaaaaaa taatcagaat agatcagtgg
    31561 cacattataa gtcagttctc tatggtatgc ttctgctccg cctccaatga tcccatcttc
    31621 atccatattc caatttgcat agtgcttttc tgtcttttgg agtatattga tagcagcttc
    31681 atgatacatt tttgatcgta cagaggaaac atatcctgct aattccagaa gaccgcatgc
    31741 agcgcatact ccagcactgg tatcaatatt cgaatctttt acagcaaaat caatgagagg
    31801 aatataacca gttttttcga ttttctgaat aaaatttact gctatctttt ctgctatagc
    31861 tagatagtac ggattttttg tgtaacgata acttaacgcg aatccatata aagcccatgc
    31921 atttcctctg ctccaacaag aattctcatt gtatccttgc ccgccaagta accgaatata
    31981 tttccctgat tctgcatcaa attctgcgat atgcccggtt gatccatctt tcctcactag
    32041 atacttagct gttgtatctg catgctgccg agcaatttta gagaacctcg gatcaccagt
    32101 ttccctcgaa gcccaataca gtaatggtaa attcatcata ctatcaataa tcatccaacc
    32161 aggtttatgg tcattccatg ctactaaata gttccctgaa atattatagc gtccagcaag
    32221 taccgtagct gcatgtaacc ctagttgtcg actatgtttg ttttgagact gttgataatg
    32281 tgcaatagcc gatggtaaga ataaaaaacc tacatcatga tgaacgtctg aaaaatggta
    32341 cagggcagat tctaataatt gttcttgttg tattgctgcc tctttgaact tttctcgttt
    32401 tgtcttgtga aagagctgcc ataaaatacc actccaaaaa ccattggtcc accagtcgat
    32461 tcctctgtcc tttattacgt ctgtatattt tccgttgtaa gaaatatatg gaattttatt
    32521 ccccattctg tctaattccg catctatctt ttcagaagct acattcaaaa tttttcttga
    32581 ccattccttc gtctgttcat tcatggatat cccgccattc ttttaatttt tatgaatgtg
    32641 gagttgctcc tagaacgaac tagatggtta acattcgtta ttttttttac ttatttgcag
    32701 ctccaatgtc attctcctta aatttagact gcgcccacac ataaatcaag accattggga
    32761 tcactgaagt tgctaaaatt agcataattg cgccataatt aattggctca cctgtactag
    32821 aaaccgtcat atttttgata attaagggaa ccgtatattt atctttatct gaaagcatga
    32881 ctaatggaaa tagatattga ttccaacttc ccataaagat aagtaaacca ctagtaataa
    32941 tcgtaggtga taaaatcggt acagcgattt tcgtaaaaat cacccactca ttggcaccat
    33001 cgattctggc agcctccatc agtgctgtag gaaattgact gctatattgt cgcatcataa
    33061 acactgaagt tgtactggca atcgaaggga tgatcacacc tgcataagta ttcgataatc
    33121 ctaaacgact cataatgtaa aataaaggaa tcaatgtggt aaaaaaaggc accatcctag
    33181 atagcataac tgtattgaac aacagtttat tccccataaa acgatacttt gctaatgcat
    33241 aacccgccat tgtagaactg gctgtagcta aaactgtact gattacagca ataacaatcg
    33301 aattccaaat aatcaaggga taattatatc tcgccatgat ggcttgccag ttttttaata
    33361 gcatatcccc aaacttgaaa ttgattccgc cacttaaaat ttcttgatta ctttgcgttg
    33421 aagctacaat catatataaa tatggcatca aaaatattaa ggcaccaaac acaataatga
    33481 cgtacgtaaa aattctgctt tgtatctttt gtttagtcat ttttatcacc tccgaattta
    33541 aattgaatag ccgataatat aacagataca aacaccatcg tccaggcaat agcagaggct
    33601 tttccaaaat caaccatttc aaaacttgtt ttatataaat tgactcctac gaccaaagct
    33661 gaattttctg gtccattgga agtcccaaag aatatatttg ggatattgaa attacccaag
    33721 ccattgatag tggctaaaac tgtggccatt aaaatgattg gctttagcat ggggatcgtt
    33781 aataaaaaga attgccgaaa tttcgatgct ccatctattt cagcactttc gtatatctct
    33841 ttagaaatcc cttgaagacc agctaaaaat aaaatagtgt tatatcctac actaagccaa
    33901 atcatcgtga tgatcattga tattcgtatc caaaaaggat ttgttaacca ctgtacaccg
    33961 ttaattccta gcgcattcaa aaatccattg aataacccag tcggattgaa aaaagtcatg
    34021 aatacggaag ctacagccac cgttgatgta atggttggaa tataatagac agttctaaaa
    34081 aatccttttc cttttaactg tgctgaattc aataaaacag caaagtaaat agctaatagc
    34141 atcgttattg gaattgatcc tgccatgata atcaaaacat tcgtgtaaga tttaataaac
    34201 aatggatctc ggaagatagc tagataattg tcaaatccaa caaattcaaa tgagtagcca
    34261 attttcataa aactaaaagc aaacgagata atgatgggaa tcaaaatcgt cacaataaag
    34321 aaaataacaa aaggtgtgac caatccataa gcactttttt tatgagacgt atctgttgct
    34381 tttttcttgc tgttctttat tgtcttgctt ggtaacgaag attgcatcat cgttaagtcc
    34441 tcctaatatt ttttaatcac cagttaattt tacgccgtac ttatgagcaa aattttctgc
    34501 ttcttttaga tatgaatttt caaaatcacc tgtttgccaa tagttatatg cagcggtagt
    34561 caaataagac gtagcatctg aataagtggc tggtaaaaca gtatttgatg tattttcatt
    34621 gattttattc aatagttcat agtatttttg tcctccttag tagtcaaact ttttctcagc
    34681 tgcttctgtt tgataggcca atgagttaga tgtagccacg cccagctcca gtgcgttggc
    34741 taagcattct tcatctgtca gcataaatgt aagaaattgt tgtgccactt gtggattttt
    34801 ggcatcttta gggacataaa aacttgatcc tcctgatact gcaccttttc ctggattttc
    34861 ttttgaaaaa ggaatcagtg gagccatgcg ccatttgtca gatgcatcag gaaaattcaa
    34921 agacatattc gtagcatacc agccgccagc aacaaacatt gcagattctt gaaatgcttt
    34981 ctcagcatct tgtgcaccgt agaaatgtac aatatctgag tcgatcattt ctttgattaa
    35041 ctttgcagca tgtttcgctt ctttagtatt cagacggata tttccttctt catcaagcaa
    35101 agattgattc tgctgggcta gcatgttgat aaacaattct tggtccccag attctggcaa
    35161 ggcaattcca aaactaccag ttttttcttt taatgctttt gccacctgga tatattcttc
    35221 ccaagaactg atttcttcaa aatctacacc tatttcagca aatttttttg gttgataata
    35281 acaaagtgct ggggtaaaat cactagccca aggaataatc gtttcattac tcgtttgttg
    35341 acgcaaaata ttcaattttt gatcataaaa ctgatcgcca tatttgtcat aaaatccata
    35401 ttcatcagca ctataaaaag catcttgaaa tttttccagc acacctgctg ctgaagtgtc
    35461 tgccatggag ataatatccg gcatatcttc gtgtgttacc aattttgggg tgatcatttg
    35521 tactgttgct gttcccgaag ctacattcac aatttttaag tcaatatttg ctccagtttt
    35581 gcggttatag atttttgcag cattttcaac atagtttgtc agtttgccgt ttgcgacata
    35641 tacttctaat gtagtggctt tttttatcat tggatctgcg atgtttgctg ttacttcttc
    35701 gttagttttt ccacatgcac ttaagctgaa taatagggta atgtttagca aagctatcac
    35761 caatttgtat ctttttttca tgtctatccc ttctttcttt tcaagttctt atataaaacg
    35821 ctttcactta tgtatattaa cggaattagt aacacaaata tttatataaa aaaatatttt
    35881 acttgtcgtt ttggcttttc atttttgtta taatagtcat taattataaa tagaaagctt
    35941 tttatataaa aagagaaagg aaagtggttc ttttgttaac aaacaaccaa tcctccaatc
    36001 ttgtcaaaaa aacactaacc tattccatag atacccatct tcatttatct gctgctccct
    36061 attttcgact actaataaat gaaagcgcta atttatatgc tgaatttgca cataaatcta
    36121 tagaactaga taatccttcc cttttattca ttgataaaca gcaagaagtc caaatctttt
    36181 ctactcaaaa ggaaacacaa acttttatca ttatccagtt ttattttgat gagcaattat
    36241 tagcacatat tacgggacaa tctttttcta tagatcaaga aataacatct atcattactt
    36301 ttctgaaaaa tgaacagttc aaaattgatt atctaaaaca tagtaagcca gctatttcct
    36361 atgatcagga aatcaattct tttttcatac tatgccaaca gatgagcatt catttgcttt
    36421 ccttattagt tacttattgt ctgcaaaaaa ttcaattgtc ttccttacca aggcattatc
    36481 aacaggaact tgctttattg acaatacctt caaatgagaa agtcattcct aaaaaaaagc
    36541 atattcttta ttcagatgaa ttagttgatc caatcatacg atatttagaa gaacatcttt
    36601 cagaaaattt tttgcttgaa caattagccc aaattttttt tatcagtcca gccagcttaa
    36661 aaaaacattt tagaaaagtg aatggaaaaa gtgttattag ctattttaaa gaactaaaaa
    36721 tggaaaaagc aaaacgtctc gtgctagaaa atgaattaac ctatacagaa attgcagata
    36781 tattaggatt tcattcgatc catcattttt cttcagcttt taaaaaatat acagggttat
    36841 ccccttcaaa gtattatcta tccttaaaat agaaaaaaat ttttaggttt tatccacaaa
    36901 ttatttctct atattttctg tctattcaaa ataaacaata attttccggc acaattattt
    36961 gctgtttttt accataacat agtacgatca acgtagaaaa ggagatgttt ttatgaaagt
    37021 tgttgtcatt ggcggtgtag ctggcggtcc ttcatttgcc actcgtttcc gtcgattgaa
    37081 tgaagcgcac gaaatcatta tctatgaacg cggagaaaat atttcttatg caagttgtgc
    37141 tttgccttat tatttaggag gtgtgatcac tgaccgtgac tcgctgatcg aacgtacacc
    37201 agaaatattg aaaacaaaaa acaacatcga tgtctttact aaacacgaag taacagcaat
    37261 cgatccttct actaagcgat taacagttaa agacctatcc acaaatgaag aaataaaaac
    37321 agattacgat aagttgatca tctcttctgg tgctagacca gattatccgg atattcccgg
    37381 agtttttgaa gcagaaaacg gctttgtcct ccgtagtgtg acggatgcgg atcgaatcaa
    37441 atcgttcctt gaagaaaaaa atccacaaca tgtcgtcatt cttggtgcag gtgttatggg
    37501 tctggaatta gctgagaatt tcaagcatcg cggcttagac gtgactttaa tcgatcaatt
    37561 gccacaagtc gctttccctt atgatccaga aattgctaat ttagtttatg acaaattgct
    37621 gaaagaagga ttagccgttc atttagaaac aagagttact gagatccgtg ataaaggtcg
    37681 agaaatagta ttatcagatg gctccgtcct ttctgctgat atgctaattt ttgctgttgg
    37741 tgtttctccg aagaatgaag tggtgaaagc aaccggcata caattatcag atacaggtca
    37801 gatcattgtt gatgaccagt tacaaaccaa tctgccggac atctatgcga ttggcgatat
    37861 tatcgaaacg actagtgtag tgactggtca gccgatccaa agtatgcttt ccagtgcggc
    37921 caatcgtcaa ggacatatgc tggcagatat tttaaatggt acgcctatgc gctatcgcgg
    37981 atatattggt gcaggtgtcg caaaaatctt tgatcataca gcaagttatg cgggaatgac
    38041 agaacatgaa ttaaaagcat caggcataac aaattataaa actgttttta tcactccctt
    38101 tgaccatgcc tatttctatc cagaagctac aagattaaat ctaaagctga tttttgatgc
    38161 agatagcggt cgtattttag gtggacaagc atttggagaa aaaggtgtcg ataaacggat
    38221 gggagaactt tctgtagcaa tcaccggaaa cttgacagtc tttgatttgc ctgatttgga
    38281 gttgccttac tctccaccgt attctactac ccgtgacccg ttgaatatag ctggttatgt
    38341 cgcaatcaat caaatgacga atatcgtaga aacgatcaaa gcaagtgata tacccgaaaa
    38401 cgatttgaaa gaagcgttct ttttagatat acgtgaacct aataaagcac cttctggcag
    38461 tatctcggca acaaaaaata ttccaatgaa tgagttacga gatcgaatca atgaaatccc
    38521 aaaagataaa aaagtttaca ttactttcag aagaggattg aatacttata cttctgcccg
    38581 aatcttggca ggtttgggta tcaaagcgat tttgattgaa gaataaaaaa acgaccgcgg
    38641 tgggtagccg cggtctagga gttattttta cttgaggagt aaaaatgaaa aagtatatag
    38701 ggttgttggt tggtatgtat atagaataga agataaatgt gaagaaattg tgcattattt
    38761 cttcacattt attaggttat agtttaagaa agtttaaaca ttgcgcaatt taattatttt
    38821 agctgtttct tttcataaaa aaacatgttg tgactctgag acaaatcata gtccatagtg
    38881 atgtctctag agtaactacg cctaatatta cgaggcatca tattaatcta tcagcactag
    38941 tacctaaact agcatccctt tcgaatattt tttcttatat aataaagttt gctttcactc
    39001 ctctaatctc accaaatcga ctggcttgcg ttgtgtgata ctaggaactg attggtttag
    39061 aacgactttt ctattgtaat catatttttc gccatcctcg ttgtcttcta atccttcaat
    39121 gaatgatccc taatgtgtgt attaaataat ttaatttttt tgttcctatt tatttaa
//
LOCUS       DAJSAW010000024        38092 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJSAW010000024 DAJSAW010000000
VERSION     DAJSAW010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.024"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..301)
                     /locus_tag="ONT64_001354"
     CDS             complement(<1..301)
                     /locus_tag="ONT64_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR2302778.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDK"
     gene            complement(303..500)
                     /locus_tag="ONT64_001355"
     CDS             complement(303..500)
                     /locus_tag="ONT64_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HCR2302779.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(894..2621)
                     /locus_tag="ONT64_001356"
     CDS             complement(894..2621)
                     /locus_tag="ONT64_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HCR2302780.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2614..3807)
                     /locus_tag="ONT64_001357"
     CDS             complement(2614..3807)
                     /locus_tag="ONT64_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR2302781.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3994..4638)
                     /locus_tag="ONT64_001358"
     CDS             complement(3994..4638)
                     /locus_tag="ONT64_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2302782.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4732..4866)
                     /locus_tag="ONT64_001359"
     CDS             complement(4732..4866)
                     /locus_tag="ONT64_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCR2302783.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4868..7000)
                     /gene="feoB"
                     /locus_tag="ONT64_001360"
     CDS             complement(4868..7000)
                     /gene="feoB"
                     /locus_tag="ONT64_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCR2302784.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6997..7470)
                     /locus_tag="ONT64_001361"
     CDS             complement(6997..7470)
                     /locus_tag="ONT64_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCR2302785.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7871..8095
                     /gene="nrdH"
                     /locus_tag="ONT64_001362"
     CDS             7871..8095
                     /gene="nrdH"
                     /locus_tag="ONT64_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR2302786.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8254..10413
                     /gene="nrdE"
                     /locus_tag="ONT64_001363"
     CDS             8254..10413
                     /gene="nrdE"
                     /locus_tag="ONT64_001363"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR2302787.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10463..11428
                     /gene="nrdF"
                     /locus_tag="ONT64_001364"
     CDS             10463..11428
                     /gene="nrdF"
                     /locus_tag="ONT64_001364"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR2302788.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11517..12656)
                     /locus_tag="ONT64_001365"
     CDS             complement(11517..12656)
                     /locus_tag="ONT64_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR2302789.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12965..13678)
                     /locus_tag="ONT64_001366"
     CDS             complement(12965..13678)
                     /locus_tag="ONT64_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCR2302790.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13932..14633)
                     /gene="nagB"
                     /locus_tag="ONT64_001367"
     CDS             complement(13932..14633)
                     /gene="nagB"
                     /locus_tag="ONT64_001367"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR2302791.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14867..16399
                     /locus_tag="ONT64_001368"
     CDS             14867..16399
                     /locus_tag="ONT64_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR2302792.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16590..17792)
                     /locus_tag="ONT64_001369"
     CDS             complement(16590..17792)
                     /locus_tag="ONT64_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2302793.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17874..18563)
                     /locus_tag="ONT64_001370"
     CDS             complement(17874..18563)
                     /locus_tag="ONT64_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR2302794.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18669..19037)
                     /gene="rplL"
                     /locus_tag="ONT64_001371"
     CDS             complement(18669..19037)
                     /gene="rplL"
                     /locus_tag="ONT64_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR2302795.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19090..19593)
                     /locus_tag="ONT64_001372"
     CDS             complement(19090..19593)
                     /locus_tag="ONT64_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR2302796.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19797..19924)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(20003..20692)
                     /gene="rplA"
                     /locus_tag="ONT64_001373"
     CDS             complement(20003..20692)
                     /gene="rplA"
                     /locus_tag="ONT64_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR2302797.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20796..21218)
                     /gene="rplK"
                     /locus_tag="ONT64_001374"
     CDS             complement(20796..21218)
                     /gene="rplK"
                     /locus_tag="ONT64_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR2302798.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            complement(21605..21844)
                     /locus_tag="ONT64_001375"
     CDS             complement(21605..21844)
                     /locus_tag="ONT64_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2302799.1"
                     /translation="MKKEYGYPTLKVICKASAGNETAIREILKFYDAYICKLCLRPFY
                     HSESGKITMQVDEELKGQIHTEMMKAILKFEIRVK"
     gene            complement(21849..22268)
                     /locus_tag="ONT64_001376"
     CDS             complement(21849..22268)
                     /locus_tag="ONT64_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2302800.1"
                     /translation="MNTSSFEHIVRIQFNALMITVIKCTVKSRNRQFARRSKREVLFY
                     ELSDTKNLECVTEDKYSCDYISFEVLNFTIQISNEKLAVALYKLSSKERDVILLRYFQ
                     SMSDQEIAELYHVSRSAIYRRRSNGLKKLKTLLKERN"
     gene            complement(22509..22913)
                     /locus_tag="ONT64_001377"
     CDS             complement(22509..22913)
                     /locus_tag="ONT64_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HCR2302801.1"
                     /translation="MKKPKVAFVCVHNSCRSQIAEALGKYLSSNVFDSYSAGTETKPQ
                     INQDAVRLMKEMYQIDMEKTQYSKLLSEIPQVDIVITMGCNVNCPTIPCKYREDWGLN
                     DPTGKNDDEFLKTIHSIHAKILELTEKIKIMR"
     gene            complement(22943..23275)
                     /locus_tag="ONT64_001378"
     CDS             complement(22943..23275)
                     /locus_tag="ONT64_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR2302802.1"
                     /translation="MKLFGKKKETKSCCCGGNCTSETMQNAENKKQEQGIKILGSGCA
                     KCMELEKATRTAISELQLDYEIEHITDFAEIASYGVMSTPALVFNGEVISYGKVLTVD
                     EVKALLSK"
     gene            complement(23292..24287)
                     /locus_tag="ONT64_001379"
     CDS             complement(23292..24287)
                     /locus_tag="ONT64_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR2302803.1"
                     /translation="MWLFFQDQILGMKWLNGLIGNGLSLLGLDINSRLGGSIQFFLYD
                     VIKITILLCLLIFFISYIQSYFPPERSKKILGRFHGIGANIISALLGTVTPFCSCSSI
                     PLFIGFTSAGLPLGVTFSFLISSPMVDLGSLVLLMSIFGAKVAFAYVIVGLIIAVIGG
                     TLIEKMHMEKYVEDFVKNASNVDISSPTLTKKDRVQYAKEQVVGTFKKVFPYILIGVG
                     IGAVIHNWIPETWIESILGSNNPFGVILATLVGVPMYADIFGTIPVAEALLSKGAQLG
                     TILSFMMAVTTLSLPSLIMLKKAVKPKLLTLFIAICTFGIIIVGYLFNIFSTLFI"
     gene            complement(24383..24688)
                     /locus_tag="ONT64_001380"
     CDS             complement(24383..24688)
                     /locus_tag="ONT64_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2302804.1"
                     /translation="MNDFSKDAKIFKALCDTKRLTILDYLKSGEKCACVLIENMNIGQ
                     SALSYHMKILCDSGIVNARQEGKWTHYSLSKSGSEYASKRLLELTTPNAENQSICCK"
     gene            complement(24916..25551)
                     /locus_tag="ONT64_001381"
     CDS             complement(24916..25551)
                     /locus_tag="ONT64_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2302805.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRTEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGTNIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYRTPYQVVKMINPNSKEDMELLREVLRNNHFDDEDE"
     gene            26061..27203
                     /locus_tag="ONT64_001382"
     CDS             26061..27203
                     /locus_tag="ONT64_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2302806.1"
                     /translation="MNNLEFALEMKNKRLASGLSQRELAGLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRLPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGTGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYKNGGAECRCKNYENVQSGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            27196..27402
                     /locus_tag="ONT64_001383"
     CDS             27196..27402
                     /locus_tag="ONT64_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR2302807.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            27481..28674
                     /locus_tag="ONT64_001384"
     CDS             27481..28674
                     /locus_tag="ONT64_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2302808.1"
                     /translation="MREKRRDNKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKRKKPSLRELEQQIRRDIEDGIDSAGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFDFKLSEVLENDTKEKVALTEEQEQALLSFIKTDNVYHKYYDDMLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKNKEQGYYIETPKTKSGIRQVPLSR
                     ETIQAFQRVMKKRPKAEPFVIDGRSNFLFVNHKGRPKVAIDYNALFVRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASRNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            28990..29658
                     /gene="sdaAB"
                     /locus_tag="ONT64_001385"
     CDS             28990..29658
                     /gene="sdaAB"
                     /locus_tag="ONT64_001385"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCR2302809.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            29671..30543
                     /gene="sdaAA"
                     /locus_tag="ONT64_001386"
     CDS             29671..30543
                     /gene="sdaAA"
                     /locus_tag="ONT64_001386"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCR2302810.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(30634..31653)
                     /locus_tag="ONT64_001387"
     CDS             complement(30634..31653)
                     /locus_tag="ONT64_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCR2302811.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(31826..32731)
                     /locus_tag="ONT64_001388"
     CDS             complement(31826..32731)
                     /locus_tag="ONT64_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR2302812.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(32790..33335)
                     /gene="nusG"
                     /locus_tag="ONT64_001389"
     CDS             complement(32790..33335)
                     /gene="nusG"
                     /locus_tag="ONT64_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR2302813.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(33436..33606)
                     /gene="secE"
                     /locus_tag="ONT64_001390"
     CDS             complement(33436..33606)
                     /gene="secE"
                     /locus_tag="ONT64_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR2302814.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(33630..33782)
                     /gene="rpmG"
                     /locus_tag="ONT64_001391"
     CDS             complement(33630..33782)
                     /gene="rpmG"
                     /locus_tag="ONT64_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR2302815.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            33944..34588
                     /locus_tag="ONT64_001392"
     CDS             33944..34588
                     /locus_tag="ONT64_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR2302816.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(34644..35147)
                     /locus_tag="ONT64_001393"
     CDS             complement(34644..35147)
                     /locus_tag="ONT64_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCR2302817.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(35160..35206)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            35744..36709
                     /locus_tag="ONT64_001394"
     CDS             35744..36709
                     /locus_tag="ONT64_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCR2302818.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(36808..37980)
                     /locus_tag="ONT64_001395"
     CDS             complement(36808..37980)
                     /locus_tag="ONT64_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2302819.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(37967..>38092)
                     /locus_tag="ONT64_001396"
     CDS             complement(37967..>38092)
                     /locus_tag="ONT64_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2302820.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT        11798 a         7723 c         6691 g        11880 t
ORIGIN      
        1 atttatctcc ataatctgct cgatataaac catttattcc agcagttctt ccaatttcac
       61 tcaaatttct tattaattcc tcttgatatt cagtgaatct tattttatta gtataagcag
      121 taaaatatag ataattcacc aatctattta ttaaaggacc gggatctaat tcatattttt
      181 gtttttctaa ttttaaatat accttttgta aaacctcaag aatatcagaa tatttaggat
      241 ctttacttat agatatatca ttatataatt catgaacaat ttgcttagca ttttttttca
      301 ttttagcact tccctggaat tgctccacct aaaaaaccac ctatagacat tcctgcaata
      361 caagtagttg ccttggccca atcgaccgta catttatttt tagtgcaata cactccattt
      421 ccataatatt ttccactatg agtggtaccc ccatagataa gttgtgtctc tttaatagac
      481 aaaattttta aatgtttcat aatctaatca cccctaaatt aatgttttta gactattatt
      541 ctaacattca ttaaatttca aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg
      601 aataagaatt taatcacttt taagtatata aactatgtat attctttaat attttttgag
      661 aatgcacttc gtacataata aactttaatc acattagtct ttcataccta cgatcgatct
      721 ctttgactcg cacccataaa ttcataatag tttgtcggcg tctttggacg tttcgaagat
      781 gagctacgca ttctcttttt ttatagacaa aaaagataga gacgaacatg gagcttccaa
      841 atttggatat ttgatccaat tttggaagct ccatttattt cgtgtctgtc tttttatttt
      901 ttccagcttt cgactaattc ttggttctct ttgatccagt cttttgctgc ttgctgtgga
      961 tcttttccat tgttgatttc caacataact ttttccatat ctttttctga ccaatggaaa
     1021 ttgtctaata ctttgtaagc ttctggctgg tcttctttca atccttttcg aaccatcgta
     1081 tggatctgct cttcgcttcc cattccgttt tcaggatctt ctaagtattt cagatcatat
     1141 ttggcaaaca tccaatgagg tgtccatcct gtaactacaa ttggttcgtg ttttttgatc
     1201 gcttgaccta gtgcaacggt cattgctcct gaagaagatg tctctacttt ccagtctttc
     1261 aagttgtcat atttttggat cgtgttttca gcagccgtaa ctacaccagc tcctggctcg
     1321 attcctatga ttttcttacc tgcctgatcg gttaactcat cgatcgaatt tacatccatg
     1381 taggctggca ctgctaaacc aacttttgct cctgtcaaat tggcacctag gtcttcgact
     1441 tgatctttat attgtgcata ttgtgaacca tgagttttag gtagccaagc tgaaaccatg
     1501 gcatctgact cgccatttga aacagatttc cacatgattg aattatctaa tggcgtcatc
     1561 gttacatcat agcccatttg tttcaatact tcaccgacta catttgttga agcaacttct
     1621 gtatcccatt caacataaga taagttgata cgttttgttt ctttggcaga tgaaaaactc
     1681 cctataccaa tcgcacctaa gatcactact actacagcac caatgatcag tccttgacgt
     1741 tttttacttt tctgtgttgt tgctcctgca gcttttttat tgtttggctg gttcaaatgc
     1801 tgtgtaaaac ggtcaatgat gattgctaag ataactaatg caaccccgtt gacaaatcca
     1861 ttcccgactt gtgctctttg caatgctgat aatacgccgc gtcctaagcc gggtgcaccg
     1921 atcatagagg caataacgac cattgaaagt gacagcattg ttgtttgatt gatccctgcc
     1981 atgatcgtac ttttagctaa aggcaattct agtttgaata gtttttgacg acttgtgctc
     2041 ccgaatgaat cagaagcttc tactaattct ttaggaactt gtcgaatccc taaattcgta
     2101 aaacgaacgg ttggtggcag agcaaagatg actgaggcaa atacccctgg aaccatcccg
     2161 ataccgaaaa atgcaactgc tggaatcaaa taaacgaatc ctggcattgt ctgcataaag
     2221 tcaaggatcg gtttgataat actttgggct ttactgcttt ttgccatcaa gatccctaat
     2281 ggtaccccaa tgatgatcga gatcacacta gataaaagaa caagtgtgac agtattcatc
     2341 aaatcattcc atagaccttg gttataaata aaccatagac cgattaatgt gaacaacgta
     2401 agtcctggac gtttcttaga aataaagaaa gctgctaccg tcaataacaa gataaacaaa
     2461 ggagctggaa tcactaacag taaatttgta atccccgaca tcactgcctg tccaatcgtt
     2521 tgtaaaaatg aaaataggcc agaaaaagtt gtcgtaaacc attctgtgat attttctacc
     2581 cagtttgcca ctggtaattg atagttcaat agattattca tgttcactca cctcgcttgt
     2641 ttctgctaat gcttcaatca cactacctcg gatgacgaca ccttttaatt ttccatcctc
     2701 tacaactgca agtggagtcg gtgagtcata aataatattg aaaatatcac ttaccaccat
     2761 gtctttccca atcaccgtca cattttcatc taggaagtcg atcaatggac gtttttgctt
     2821 cctagcttct aacgcttgat ctgcagtgat gatccctttc aggtgtctct ttctgtcaac
     2881 agccatcagc atgctgactt cttcattacg catccttgtc aatgcaacgt ttggtccatc
     2941 agattcgata ttcgtcgtca atgcaggtac catgatgttt tgagccgtaa gcactttgga
     3001 acgatcgact tcttccacaa attcacgtac ataatcattt gctggattgg tcaatatttc
     3061 ttcacctgtt ccgatctgca tgatttcgcc gtctttcatc aaggcgattc tgtccccaat
     3121 ccgtaaagct tcattcaaat catgggtaat gaaaataatc gttttttgaa cattggcttg
     3181 caggtccagt aattcgtctt gcatttctcg ccggatcagc ggatcaagag ccgagaacgc
     3241 ttcgtccatc agcaagatct caggatcatt tgctaaagct ctggctaggc caacccgttg
     3301 ttgcatccca ccagataact gactaggata ttggtcttta aatgaaagca aactagaatt
     3361 ttctaatgct ttttctgctt tttcttgtcg ttcttcttta ggtacaccac ggacttctaa
     3421 cccgtattct gtattttcca agatcgttcg atgtggaaac agaccgaagt tttggaaaac
     3481 catattgatt ttatgacgac ggacttctcg caattcttct ttgcttaact tcgatacatc
     3541 ttctccatca atatagatac tgcccgaagt tggctcaatc agacgattca atagacggat
     3601 caatgtggat ttcccactcc cggaaagccc catgatcacg aagatttctc cttcttttac
     3661 gtcaaaattc acgtcataca caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt
     3721 cggctggtga tcattcatca tatctaaagc ggcttgtgtc tttttaccaa atatctttgt
     3781 taaatgtttt acttctactt ttaccaaaca aattctcctt ttttatgtaa attcattttt
     3841 ctttctgaaa aaagtgacca cactatgtta acatcatgta gtcactttgt caaaaatgaa
     3901 agaaatttgc ttgtccaaac tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta
     3961 gccaagttaa tattttttta atatttcaca caatcattct gcaagctgat gctccgggta
     4021 aaaaaagttt gtcatacgct gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc
     4081 tgccgtaaat ttagcatagg ggccaggtga aacaagcagt tcttcattat gagaaattgc
     4141 gataactgtg gcgccagtcg cctgccagat attcaactcg ctaattgaac ggtccagatg
     4201 cattgctttt tctgataaag ataattcaaa cggaagtaaa ggattcatct tatgtattcg
     4261 cttcgtttga ttgaccagct gatccaataa ttgagaaaaa tgattcagct catcttgttg
     4321 cttctccaca cttgccatga tttctgcttt tgtatcttgg atggattgta catcttgata
     4381 ttgcaatagg aaatcttttg ctttttcctt cgaagcaaca aatgccccac ttccatgccg
     4441 tacttccatg attccaagat ctaccagcac attgatagcc ttgcgggtag tctcaggaga
     4501 agcattgtac gtactggcaa gtgttgagcg ggcatggatc ttctcaccca ctttatatcg
     4561 gttttcaacg atacgttcgg ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat
     4621 ttttttcttt ccagacattt tctttcctct ttcttttttt actttccctc atcttactat
     4681 gctctagcaa aataagcaaa aaaggggaga atagcctctt gcaacgactg atcatcgctt
     4741 gatctgttcc ttttttactg ggcaagctgt ttgacagtca tcacaattct taccggattt
     4801 taaacgagta tagacaattg cgcctgcact tccaaagatt aacacactta ataagaatgt
     4861 cgccatccta ttcctctcct tctaatgaca atatagtata aaactggttc ttggattttg
     4921 gctgacggaa caaaacataa acgagtccta tcatcagtaa aatagcaaga tacgtcccta
     4981 acgctacctg tccaccctcc aataagtgtc ccaactgata aatcacaaaa ctaactaaat
     5041 aagctaaacc gcattgatag cctatggcga tccaagtcca tttcttggca ttcatttccc
     5101 gatggatagc cccaattgcc gcaaaacaag gcgcacaaag taaattgaag gctaacaatg
     5161 aataagctgc tgcgggagta tatgtgtgtt gtagcacttg ccagatttct ctgccatttt
     5221 cggatacttc cgcatgacca aacaaaatac caaatgtgcc aataatattt tctttagcga
     5281 ccaatcctgt aatcgctgcg actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa
     5341 caactgccag gattcgcccg aaaaaggcta agatactttg gtcttcagaa acaggttgag
     5401 cataaaaatt ataagaagag ctgaaccaga tcacgatact agaaacaaag ataatcgttc
     5461 ctgctttttt aacaaaagac ttgctgcggt cataggtttg tcttaggacc gctgaccatt
     5521 gaggaaaatg atatgcgggc aattccatga taaaaggagc tgtatctcca gcaaacaaac
     5581 gtgttttttt caagctgatt ccagagaaaa taattgcagt cataccaaga aaataggcag
     5641 aaggagctac ccaagattga tgaggaaaaa aggcccctgc tactaaggca ataatcggca
     5701 actttgcaga acaaggcatg aaggttgtga ccatgatcgt caaacgacga tcttgctcgt
     5761 tttcaatcgt gcgactggcc atgacgcctg gcactccaca tcctgtagca atcagcatag
     5821 gaatgaagga ttttcctgaa agaccgaatt tcctgaaaag tcgatccatg acaaaagcga
     5881 tcctcgccat atatccgcag tcttccagaa agctcaagca taaaaacaat ataatcaatt
     5941 gcggtagaaa tcctaacact gctcccacgc ctgcgatgat gccgttcaag atcagctcct
     6001 gcatccatcc cgctacttgc cattcagcta acaatctgcc aacatgttct ggtaaccatg
     6061 taccaaacaa tgtgtcattg atccaatctg tccccattgt tcctactgtt tgaatagcca
     6121 gataatagat cagccacatc acaaatgcga agatcggcaa ggctaaccaa cgattcgttg
     6181 caatctgatc gattttgtca ctcatcgata attggaaaga atcatttgac acaacacaga
     6241 gtgtacatag ttgggtaata aattcataac gttcattgac cagaatcgtc tcactatcgt
     6301 cgtccaatag tttctctgtt aactggatca gttgctcgat ctctttttgc tgagaaactg
     6361 ctatctcgta atcctctctt accttctgat ctcgttcaaa atatttcatt gcataccagc
     6421 gtttcttgcc gctttgttct cctagtacat cttcaatttc atgtaaagct gcttctaaac
     6481 gtggatcata tgtacaggga acaggaatag aaggccgctt ttttgcagac tcctgaatct
     6541 tttcgattag tttttcgatt ccttgctttt tcaatacgct gattggtaaa actggtacac
     6601 caagaccata ggaaagcttt tctagattga ttttttttcc ttcttttttg atgacatcca
     6661 tcatattcag accaatgatc ataggaatgt ctaaatccaa taactgcgta gttaaataaa
     6721 gatttcgttc taaattggta gcatcaataa tgttgatgat cagttcaggc tgttcctgaa
     6781 ccaaataatc tcttgcaatc acttcttctg gtgtataagg agacaaagaa tagatgccgg
     6841 gtaggtcctg aatatgaatg ttcggatttt ttttcaacga tccttctttt ttttctactg
     6901 ttacgcccgg ccaattccct acatattgat tcgaaccagt caagagattg aacaagctag
     6961 ttttcccgct gtttggattt ccgactaacg cgatattcat tctttttcct ccctttgtat
     7021 caagattagt tcggcctctg actttcgtaa cgtcagttca taccctcgca ctgtcagttc
     7081 gattggatct cccagagggg ctagttttcg gataactatt tttacatttc gcgtcaatcc
     7141 catatccatt aaccgtcgct tcactgcgcc ttgtccgtgg attgctacga cgatcccagt
     7201 ctctcctgct tttaattgat caagagaagt ccagctttca gaggaagcag tctttttatt
     7261 gacaaagatt tgacctagta tttcccgact tactgccact cgatgacttt ttaccagtaa
     7321 aatggcattg ttgcctgata aattgacgac gacgatttta cttccggcaa cgatcccgat
     7381 attttgcaaa tggcgttttg ttttttcttc tgtttcgatt cgttcgactt gataaacgaa
     7441 attctgcttt gcctgcgcta aggtctgcat attgattcct ctcttgtgaa ataaatcatg
     7501 tattaaaatc attcattata tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat
     7561 cattctcaca tagaatactc tcggtaattg agaatgtcaa ttatacttgt gatattatga
     7621 gcgaatcttt agtattgata gataaaaatg agaaaaaaat atttcttcat agttttccac
     7681 aattttttct ctattaaatt tgctattttc ccacacgata cttgttttca gctctttttt
     7741 caaaaacaca aaacttagta ttccacagtt tccacaacac aatatgtagt gtttgttgtt
     7801 tgactttttt actaagagtt ttatactatc tattatagat aataagaatg tgttagagga
     7861 ggcactcacc atgaacgtaa aaatcttttc taaaaataat tgtatgcaat gtaagatggt
     7921 caaacggttt ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc
     7981 tgaagcagta gactggttga aagcacaagg attccaaagt gttcctgtca ttacttctga
     8041 tgcagaaaca gttatcggat tccgtcctga tcagttgcgt aaacttgcca gctaacatga
     8101 ctaaaagaat caaaacgtga tacatggatt tgaagggctt gagacagcac cagtttttgt
     8161 cgcaaacttc caaatccttt tttaaagctt aaaaacaatg caatgatttg caatcaagca
     8221 tcatcaaata cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc
     8281 agttatttca agctgaataa tgaaatcaat cggccagtca atggtcaaat ccctttgaat
     8341 aaggataaag aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact
     8401 tttctatcta ttatggaaaa aatcaattac ttgatcgatc aagaatatat agaagcagaa
     8461 ttcattcaat tgtattcacc atcatttatt gaaaagctat atcaattttt ggacgagcaa
     8521 catttttctt tcaaatcttt tatggctgct tacaaatttt attctcaata tgcgttgaaa
     8581 accaacgacg gttccgctta tctggaaact tatgaagatc gagtagcctt caatgcgctt
     8641 tactttgcga acggggatga agaattagca cttgctcttg cagacgaaat gatccatcaa
     8701 cgatatcagc cagctacacc ttctttcttg aatgctggac gtaaacgtcg gggcgaattg
     8761 gtgtcttgtt tcttgactca agtcactgac gatatgaact ctatcgggcg ctcaatcaac
     8821 tctgctttgc aattgtcccg tattggtggc ggcgtaggga ttactttatc aaatcttcgt
     8881 gaagccggcg ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg
     8941 aaattattcg aagatagttt cagctattct aatcaattag gtcagcgtca aggagctggc
     9001 gtggtttatt tgaacgtttt ccatccagac attattgctt tcctttcagc aaaaaaagaa
     9061 aatgcagatg aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa
     9121 ttttatgaat tgacacgtaa aaacgaagat atgtatttat ttagtccata tagtgtcgaa
     9181 aaagaatatg gtgtcccatt ctcttatgta aatattacag ctgagtatga tcatctagtt
     9241 gcaaatccaa acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca
     9301 aaattacaac aagaatctgg ctatccttac gtaatcaata ttgatacagc gaaccgcgag
     9361 aatccaatcg atggcaagat catcatgagt aatttatgtt cagagatttt acaagttcaa
     9421 acgccttctg tgattaacgg caaacaggaa tatgaagtat taggaacgga tatctcatgt
     9481 aacttaggct cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg
     9541 cgtacgatga ctcgtgcatt gacatttatt acagatgcat ctgatatcga tgtcgtacca
     9601 ccgatccaaa acggaaataa gctgaatcat acgattggat taggtgccat gggcttacac
     9661 acttatcttg caaaagaaca aatggaatat ggctcagagg attcattaga ctttacaaat
     9721 atttacttca tgctgttgaa ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa
     9781 agaaaacaag ttttccataa ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa
     9841 aaatacgtaa ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc
     9901 atttttatcc caactgctga agattggaat gctttgaaac aagcagtaat gaaagatgga
     9961 ctgtatcatc aaaatcgctt ggcagtagca ccaaatggct caatttcata tatcaacgat
    10021 acgagtgcaa gtattcatcc tatcactcgc atgatcgaag aacgccaaga gaagaaaatc
    10081 ggtaaaattt attacccagc gccttatcta tccaatgaga cgattccgta ttacacttct
    10141 gcttatgata tggacatgcg taaagtaatc gatgtttatg caatggcaca acaacacgtc
    10201 gatcaaggca tgagtttgac tttattcatg cgttctgaaa tcccagaagg catttatgaa
    10261 tggaagacaa caacgaagca aacgacacgt gatttgaata ttttaagaca ttacgctttc
    10321 cacaaaggaa tcaaatccat ttattacgta cgtaccttta ctgatgatgc agaagaaatc
    10381 ggaagcaatc aatgtgaaag ctgcgtgatc taatatcata gatagattcg cttattcata
    10441 ttttaattaa aggagcattc tcatgtcaga aacatattat gaagcaataa actggaatga
    10501 aattgaagat atcattgata aatccacttg ggagaagctg acagagcaat tttggctaga
    10561 tacacgtatc ccactttcaa atgacctaga tgattggcgt acattgtctc aattggaaaa
    10621 agatacagta ggacatgtat ttggcggttt gacactgcta gatactgtcc aatctgaaag
    10681 tggcatggat cagttgcgta aagatgtccg cactccccat gaagaagccg ttttgaacaa
    10741 tattcaattc atggaatccg ttcatgccaa aagttattct tctattttta gtacactgaa
    10801 tacgaaaaaa gaaatcgaag aaatttttga ctggacaaat acgaatcctt atctgcaaaa
    10861 aaaagctgag cgtatcaatg aaatctacaa acacggtaca ccgctagaaa agaaaattgc
    10921 cagcgtcttt ttagaaacat tcttgttcta ttcaggtttt tatactcctc tttattattt
    10981 aggaaacaac aaattggcta acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc
    11041 tgtacatgga acctacatcg gttacaaatt ccaattagga ttcaacgagt tacctgaagc
    11101 agaacaagaa acgttaaaag actggatgta caatcttctt tatgagcttt acgaaaacga
    11161 agaacgttat acagaagaat tatacgatcc aatcggctgg acagaagaag tcaagacatt
    11221 cctacgctat aatgccaaca aagcattgat gaacctaggg atggatccac tctttccaga
    11281 tactgccaat gatgtcaacc caatcgtaat gaacgggatt tcaacaggca caagcaacca
    11341 tgacttcttc tcacaagtcg gaaacggcta tttgttaggt catgtagaag cgatgaaaga
    11401 cgacgattat ttgattggat tggaataagg atttatttta attcaaaaat ttttcgaaaa
    11461 ataaaaagag tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat
    11521 tcacttgttt cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt
    11581 ctcaataaat agattttccg gatatttaat attatcgtct taatcactgc atagacaggt
    11641 atgcaaatca gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg
    11701 atcgtcaaag gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca
    11761 tctaattgtt ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata
    11821 aacaaaccga caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata
    11881 ttcgtgattc cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt
    11941 gccaaaaagc tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca
    12001 gataatgttt cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga
    12061 aagaagtgag acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata
    12121 aatggaacgg taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca
    12181 cttgcagata aactgactaa tatgatattg aacaaattac taagagataa atttgctgat
    12241 gctaattgtt cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac
    12301 catttgcttg tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc
    12361 attaactgag gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac
    12421 gtcaataaaa aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga
    12481 tcgaacatat aatagataaa accagcaaca agcaatggta aaaaaactgc cgaaagcatt
    12541 ttcaatattg gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg
    12601 agtactagct ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat
    12661 ctcctccaag gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga
    12721 ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact
    12781 taaacaaaaa cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat
    12841 aaacggtgtt caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga
    12901 actattttta tttggctggg cttattactt gatgtaggac atctatttta caacctcaga
    12961 tcatttattg cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact
    13021 cctcgatcac atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt
    13081 acactcggta gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt
    13141 tctcttcaaa ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt
    13201 ctaacttttt agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa
    13261 tcccgataaa actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc
    13321 cagcaattgc ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga
    13381 tgatcgttcc cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt
    13441 catctgaacg aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac
    13501 tagcgatctc aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg
    13561 tacgcatacc tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata
    13621 gcatagcgat ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct
    13681 aaacaccctc cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt
    13741 tacctcttac gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa
    13801 cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat
    13861 ttttcctttg tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct
    13921 actattgaaa attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg
    13981 tgattttgca atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg
    14041 attgcatcag ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt
    14101 gaaccgattc ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt
    14161 ttgtttgcat tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa
    14221 gcaccaggtt cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg
    14281 attggatgtg catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct
    14341 tttccatctg gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg
    14401 aaatgacggt agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca
    14461 gaagtcattt cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt
    14521 gtgcttcctg ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt
    14581 tcaaatgctt tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt
    14641 gctcctttat attttggtat agcccaatta tagcattctt tttttctttt tacaataatt
    14701 agtatagacc aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg
    14761 tttcataaga tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac
    14821 tgtggacgct ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac
    14881 attaaacaac attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca
    14941 attaaatgct ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt
    15001 gattaaaatc atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa
    15061 tattcacaaa ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga
    15121 atttctaaaa actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta
    15181 ccaaacatat agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac
    15241 ggaattagac caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg
    15301 gttggggatc gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg
    15361 ttcaaaggtg attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga
    15421 accaaccaat cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt
    15481 tgacggtgcg tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca
    15541 tatcgcagac atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt
    15601 gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca
    15661 aatcgaaaag accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc
    15721 aaaaagccga caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag
    15781 taaagctgtc tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac
    15841 tgatttagtc atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt
    15901 tggtgaaaat attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac
    15961 actgatcggt gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat
    16021 caattacttt tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt
    16081 aagcagtcaa tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg
    16141 gttagttaat caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa
    16201 ggtgaaactt gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccgac
    16261 gaaccatatc gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg
    16321 aacagtaatc atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat
    16381 tgagattgaa ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa
    16441 gttttgtcac aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta
    16501 gctcaaaaaa cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg
    16561 cagaacagct tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct
    16621 ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt
    16681 acacctaacc agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct
    16741 ccaataaagt acataaacat aaatacagta ttatttcggt tgcttgctgc ttcacctagc
    16801 ccttgtactc gtgtttgatt ggcgacttga ccaaattgat tcccgacgtc taataaaata
    16861 atagctaaaa gcaaaatggc caaatggctt ccgccaacca ataaagcaat aaagcttatt
    16921 gtttgcataa caagtcccat atacacgatt tttctttcag aataagaatc tgccagtcgt
    16981 cccacgatag gtgcagcaac tgctccagac aaaccaaaaa tcgctaatat ccctgcttcg
    17041 aatgttcccc aattgtaggc gggactgctg atataaaaaa tcaacgtaga ccaaaagatc
    17101 gaaaatgttc caaacatgaa aaatccactg actgccgctt ctcttaatag acgttgagaa
    17161 gcaactaaat gcggcaaact tttaagtgaa tcaaggtatg aaacagctgt actactttgc
    17221 tggacagaac gcggcatttt ccagtaaagc agaagtgtaa gaattccgac tgcacataaa
    17281 gcaaataaat agatcatccg ccaagaaaaa agactggcaa caataccaga taccgtccga
    17341 gaaagcaaaa tccccacgag taaaccgcta agcaatgtac ccatgacctt ccctcttgct
    17401 tttggtcccg ctagtaccgc accgtaagga atgatgattt gcggcacgat cgaaagtaat
    17461 cctattaaaa aagaagcgca tgcaaataac gtaaacgaag gcgcaaaaaa agcggccaac
    17521 agagagacgg cagataacgc tgccattcga atgatcaatt ttggacgatc caccacgtcc
    17581 cctaaaggaa ctaaaaacag taaccctaac gcatagccta attgggtcaa catcgtcaat
    17641 atcccgactg cacttgtgct aacagataaa ctagctgcta cttttgttcc aatcggctgg
    17701 atgtaataca tattggcaac aacaacgcca caagtgattg ataatagtaa aataagcgaa
    17761 gttgttaaag gttttttttc attactcttc attgatttcc cccatttcct atcttagttt
    17821 acaacttatg gaaaagattg gaaatagaaa aatacaacta ttcgccacac ttattatttg
    17881 tgcttttgtt ttccttgttt tttcttgcga tctttataaa tgaagacaac taaccctact
    17941 agcacagtaa cacttgcaac caacgtgact gcagttttgt tatatccgtg aaacagagct
    18001 tctccaccac acgcatacaa cacagacgaa gggatgactc caatcacgat cgacaatatg
    18061 atttgctgta gaggcaattt catcgcatct gcagcaaaat tgaccataga agatggaata
    18121 accggtatca tatagcctaa cattacacct atttttggat gtttcatttg tcggattgct
    18181 tgcacccaat gattcgtttc ggttttctta tctaaaaatt ttaagcgatg cataaggaaa
    18241 attgcagtga tgttaccaag agcattaccc aaaatgttga tgatcgcacc tgtcagtggt
    18301 ccaaagctca aaccagcaat gactccaact actgaagtag gaatacctgg aacagcacac
    18361 atcaccgctg tcaatacgat caagagtatc gatgcaaaag tcccatgcga acgaatatgt
    18421 gcgagtagct ctgccttact cgctctcgga tttaaaaata attggatgtc tgtctgatat
    18481 tctaaataca accgatagcc cactaccagt atcagaatga ttcccaacga gatcaatatc
    18541 aatcggatta ttttcttttt catatttcct cctgaaaaaa atctatattc attaaataat
    18601 agtttaacat aaaaacaaaa aagcaccgaa atccaacata ggatctcagt gcttttaaat
    18661 agtaaaaatt atttaacagt tacagatgcg ccaacttctt caagtttagc tttcaattct
    18721 tcagcttctt ctttagatac gccttcttta actggtgcag gagcgccatc aactacagct
    18781 ttagcttctt tcaagcctaa accagttgct tcacgaactg ctttgataac tttaactttt
    18841 tggtctccag ctgcagttaa ttctacagtg aattctgttt gttcttcagc agcagcagcg
    18901 ccaccagcag cagctacagc tacaggagca gcagcagata cgccaaattc ttcttcaata
    18961 gctttaacta ggtcgttaag ttctaggata gttgcttctt ttaactcagc aacgatgttt
    19021 tcaatgttca atgccattat aatttcctcc gtttaattgt tcttttttat ttaatgcttt
    19081 atgcggcaat taagctgcat ctccatcttc tttgtctgcc actgctttga cagcgtaagc
    19141 cacgttgcgg actggcgctt gtagtacaga tagcaacata gatagaagtc cttcgcgatt
    19201 tggcaatttt gccaatgctg tgatttcttc caatgaagag actttacctt cgatgatacc
    19261 gcctttgatt tctaatgctt ttgcttcttt agcaaattcg tccatgattt tcgctggtgc
    19321 gactacatct tcgttactga atgcaacagc tgtaggtcca gtgaatactt cgtccatgcc
    19381 ttcaaggcca gcttttttag ctgcacgtga aagaatagaa tttttgataa ctttcatttc
    19441 tacgttagca tcacgaagtt gtttacgtaa acgtgttact tcatcaactg ttaatccgcg
    19501 gtagtcaacg actactactg atgcagctgc tgtaaatttt tcgcttactt catcaacgat
    19561 tgctgctttt tttgcgataa ttgcttcact catttttgag tttcacctcc tgaattttga
    19621 tggaagagtt ttttgcactg aaaaggcaag tcacttttat agttactttg ctcaaccagt
    19681 attattcatc acctattcac ttcgttcata gtgattctac tcgctttgct cgaaaggaaa
    19741 ctcctttcgc tttgctcaac cagtattgtt catcatctct tcacttcgtt catagtgatt
    19801 cacaataaaa aactctatgc caccgcagac atagagggac tattgaatat attcaataaa
    19861 cgtcctcggt aggaaattaa ggcttgatgc cacctactgt cttcggtaca agttaattat
    19921 taacctcaac tagcttagca tgctaaacta gttgagtcaa gaattatttt tcagttggat
    19981 caatcaacat tctaatattt aattagaaag aagcttggtc aacatggata ccaggtccaa
    20041 atgttgttgt gatcgttaca tttttcatgt attgaccttt agctgctgat ggtttagctt
    20101 ttagcaatac atcatggatt gctgcaaagt tttcaactaa tttttcatcg tcaaatgaaa
    20161 cttttccgat tggtacatgg atatttccag ctttgtccac acggtaagtt actttaccag
    20221 ctttcacttc gtttactgct tttgttacgt ccattgtaac tgtaccagtt ttagggtttg
    20281 gcattaagcc ttttggtcct aatactcgtc ctagacgacc tacagtagcc atcatatcag
    20341 gtgttgcaac aactacatca aagtcaaacc atccgttttg gattttttga accatgtcgt
    20401 cgtcacctac gaaatctgca ccagctgctt cagcttcttt tgctttgtct cctttagcaa
    20461 aaactaaaac agtttgtgtt ttaccagttc cgtttggtaa tacaacagca ccacggattt
    20521 gttggtctgc ttttttaggg tctacgttca aacggtatgc aacttcaact gttgcatcaa
    20581 attttgcaat gtttgtttct tttgctaaag ctactgcttc ggcaacagga taaactttac
    20641 ttgaatcaac tttttttaat gcatcttgca tttttttgct ctttttagcc attttggttc
    20701 ctccttgatt gtggttataa cggttgtacc tcccacgtgt tctatatctt tcaatataga
    20761 gccaactgag aagctgctgt cttagggtgt atagattatt ctacagtgat ccccatgctt
    20821 cgtgcagttc cttcaaccat gcgcattgct gcttcaacgt tagctgcgtt taggtcttcc
    20881 atttttaatt cagcgatttc tcttacttga tcgctagaaa ctttcgcaac tttatttttg
    20941 tttggttcgc ctgaaccttt ttcgatttta gctgcttttt tcaatagaac agcagctggc
    21001 ggtgtttttg taacgaatgt gaatgaacgg tcttcgtata cagaaataac aaccggaatg
    21061 atcaaacctg cttgatcagc tgtacgagcg ttgaattctt ttgtgaatcc catgatattg
    21121 atacccgctt gacctagtgc aggacctact ggtggagctg gagttgcttt acctgcagga
    21181 atttgcaatt taacgatttt ttctactttc tttgccacga gacatacctc cttaagtccg
    21241 tgatgtggtt aattggagat gcatatttct cctcccacgt tttatctgat ctgaaaaaat
    21301 ccagacaaat aatgtgcaaa acaatgcaca ctggtacagt atagcaaatt tatttgtttt
    21361 ttcaagattt ttttctgcta tttttacatt ttgcttgaat aaacgctaaa tttaatataa
    21421 attttatatc tcattttcaa ttatccgcat aaatactata cattcaagta ttttaaggta
    21481 tagaaaaaca ctcattttac cacgaattta ccacgattga atgagccttg atatgacaca
    21541 aaaaacaaca tgggagatag cacaaatatc tgtgtatctc ccgtttttca ttcatgtaat
    21601 atgattactt tactctgatt tcaaatttca agattgcttt catcatttct gtatgaattt
    21661 gaccttttaa ttcttcatca acctgcatag taattttacc actttcagag tggtaaaacg
    21721 gacgcaagca taatttacaa atataagcgt cataaaactt caaaatctct cttatggcag
    21781 tttcatttcc agcagacgct ttacatatta cttttaatgt agggtagcca tattcttttt
    21841 tcattgtttc aattcctttc tttcaataat gtttttaatt tcttcaatcc gttacttctt
    21901 ctgcgataaa tagcagaacg tgatacatga tataattctg caatttcttg gtcgctcatg
    21961 ctttgaaaat agcgtaatag aattacatca cgctctttac tcgataactt atataaagca
    22021 acagcaagtt tctcgtttga tatttgaatt gtgaaattca agacttcaaa agaaatataa
    22081 tcacaagaat atttatcctc agttacacat tcaagatttt tcgtatctga taattcatag
    22141 aataaaacct cacgcttgga acgtcttgca aactgtctat ttctgctttt tactgtgcat
    22201 ttaataacag ttatcatcaa agcattaaac tgtattctga cgatatgctc gaaagaagat
    22261 gtgttcataa tctcaccccc tttctgcata cgccgaaata tataaacggg aataccgcct
    22321 ataccttttg gtaatgtaaa aaagaaagca agaaaaaagc ccgcacaaaa catatagtct
    22381 gcacgagcct tcaaaatttc cttatattca gatgttgata attgccatat tagactgttt
    22441 aatagtgtgc ttaaaacaat ttatgacaat attcttcgtt aatattttca tttagtttct
    22501 ccttgaaatc aacgcattat ttttattttt tctgtcaatt caagtatttt tgcatggatt
    22561 gaatggattg tctttaaaaa ttcatcatca tttttccctg tagggtcatt caacccccaa
    22621 tcctctctat atttgcatgg tattgtagga caattcacat tgcaccccat tgtaatcaca
    22681 atatctacct gtggtatttc agataacaat ttagaatatt gtgttttctc catatctatc
    22741 tgatacattt ctttcattag acgaactgca tcttgattga tttgtggctt tgtttctgtg
    22801 cctgcggaat aactatcaaa aacatttgaa gaaagatact tgcccaatgc ctccgcaatt
    22861 tgactgcgac aagaattatg aacacagaca aaggctactt taggtttttt cataaatgaa
    22921 ttaccaacct ttctttgttt tattatttgc tcaataatgc ttttacttcg tctacggtca
    22981 atacttttcc ataggatatt acttcgccat taaacaccaa tgcaggtgta gacattactc
    23041 cataactagc gatttctgca aaatctgtaa tgtgttcaat ctcataatct aattgaagtt
    23101 ccgatattgc tgttctagtt gctttttcca attccataca ttttgcacag cccgaaccta
    23161 atattttaat gccttgctct tgtttcttgt tttctgcatt ttgcattgtt tctgatgtgc
    23221 aatttccacc acaacaacat gattttgttt cttttttctt tccaaataat ttcatttctt
    23281 attcgctcct tttaaataaa taatgtacta aaaatattaa agagataacc tacaataata
    23341 atgccaaacg tacaaatggc aataaaaagg gtcaatagtt taggctttac tgcctttttc
    23401 agcattatta aagacggcaa actcaatgtt gttacagcca tcataaatga taaaattgta
    23461 cctaattgtg caccctttga aagtaatgct tctgctactg gaattgttcc aaaaatatca
    23521 gcatacatag gaactccgac aagagtggct aaaataacac caaacgggtt attactgccc
    23581 aagatacttt caatccatgt ttcaggtatc cagttatgga taactgctcc gatacccaca
    23641 ccaatcaaaa tgtatggaaa tactttcttg aatgttccta caacttgttc ttttgcatat
    23701 tgaactctat cctttttggt taaagtagga gaactaatat cgacattact tgcatttttc
    23761 acgaaatctt ccacatattt ttccatgtgc attttttcaa ttaaagtacc gccaattaca
    23821 gcgattatca agcctacaat cacataagca aaagcaactt tagccccgaa aatactcatt
    23881 agcaatacaa gactccctaa gtcaaccata ggcgaagaaa tcaaaaagga aaaagttact
    23941 cctaacggta atcctgcact tgtaaaacct ataaacaacg ggatagaaga acacgagcaa
    24001 aatggtgtaa ctgttcctaa caatgcggat atgatatttg caccgatacc atgaaatcgc
    24061 cctaaaattt ttttacttcg ttcgggtgga aaataacttt gaatatacga tataaagaaa
    24121 atcagtaaac ataacaagat tgtgattttg attacatcgt ataaaaagaa ttgaatactt
    24181 ccacctaaac gactgttaat gtctagccct agcaatgata gcccattacc aattaagcca
    24241 ttcaaccatt tcatacctaa tatttggtct tgaaaaaata gccatattga atttactact
    24301 tccaataaag tccctccttt acatgacata tcaatttttt ttgatgtgat aaagttttat
    24361 aaaagccacc tttcgatgac ctttatttgc aacaaattga ttgattttca gcatttggtg
    24421 ttgttagttc taacaatcgt tttgacgcat attcacttcc acttttactt aggctgtaat
    24481 gagtccattt tccctcttgt cttgcattaa caataccaga gtcgcaaagt attttcatgt
    24541 ggtaggataa agccgattgt cctatgttca tgttttcaat cagaacacac gcacattttt
    24601 ctccgctctt taagtaatct aaaatcgtaa gtctttttgt atcacaaaga gccttaaaaa
    24661 tttttgcgtc tttagaaaaa tcattcatta ttatcactcc tcacatcaaa taattttgat
    24721 atatttatca taaggcttac aacatgattt gtcaacacat tacatcaaaa tattttgatg
    24781 tattattctt ttttcctctt gattacccat aacagaaaag aaaaagccct gtcaagagct
    24841 tgccaccatt ggtggtgctt tgcactcttt actgggcttt ttgtttgctg tatcttccat
    24901 gcaagaggaa aaagattatt cgtcctcatc atcaaaatgg ttattcctta atacttcccg
    24961 taataattcc atatcttctt ttgaattagg gtttatcatt ttgaccactt gataaggtgt
    25021 cctatattta tgtccctcta tgctttcccc aaacatatcc atgctgtata aatcatcata
    25081 gcggtctaac agtttttgaa acttcaaatg ctggataaat tctttgattg ggtaaaggtc
    25141 ggacgcttca atgtttgttc cattgatgta gtcggtaata tattctcgta acttttctaa
    25201 ttcatattcc agccattctt cctcgctgtc aaagaagttg tcataatgtc catgttttaa
    25261 ttctgcattt agacgttctt ctgttcttaa aaactgacag acttcttttt cggactgatt
    25321 gacatatttc cattcccccg acaacaattc attcggcgta acgtccagca cttccattat
    25381 cttttccagc gtttcataag caggatagtt caaccctctt tcaatcttgg aaaggctctg
    25441 catattgata ccaattttgt ccgcaagttc ctgttgtttc attcccctaa attttcttat
    25501 ggtctgtata ttatttccta aatggcttat tttcattgca taaccctcca taactcgtta
    25561 tatcttcgct gctataagtc tatcatgctt aatatgattt gtaaagataa aaaaagatat
    25621 tgacaaataa gcatataaga attactatgt tcttgtaatc aaataagcac aatagaatga
    25681 aatacaaaaa cattcaaaag aaaatatcta tctgattaaa cccgtagata tgaatatcgg
    25741 ttgaaatgga acttgtttga aaggacaaac acaggacttg ccgacagagc attgtaggac
    25801 tgttggcgtg cgtcagcaac aacgccatga cggcttgccg tcaaaggacg gaaatgcttt
    25861 gtcggctggc gaacttgtga gccttgtctg tgcccccgtt atctaacagg ggggcactat
    25921 ttcacaaaag acaagtcaaa aaccatacaa ccccaaagct gtaaggagta atgaggagtt
    25981 ttaagaagat agtaacttca cattcttttg aatagtggtt tacaggtatt tcaaggctgt
    26041 tgtgggattg gaggaataca atgaacaatt tagaatttgc acttgaaatg aaaaacaaac
    26101 gtcttgcaag cggtctttca caaagagaac ttgcaggtct tacccatgta tcaagatata
    26161 gtatcaaccg ctttgagaac ggaaaagcca acgccagcaa ggaaacacaa aatattatcc
    26221 tgcgttgtct gaattactat atctgcgata agccttttta tctgctcgtt gattatctgt
    26281 ctgtccgttt accaacgact gacgcattag aagttatccg aaaggtgctt ggcatgaaag
    26341 cagattattt tatccattat gactatgggt attatggcta taaggagcat tacgcctacg
    26401 gggaaatcaa ggtcatggct tctgatgatg aacacatggg cgtatttttg gaattaaaag
    26461 ggacaggctc acgcaacatg gaatatgtct tacaggcaca gaacagggat tggtattcat
    26521 ttttaaaccg ctgtcttgac tgtggcggtg ttatccgacg ttttgatttg gcaatcaatg
    26581 atatgtgcgg tctgctggat attcccgttt tgtctgaaaa atataaaaat ggcggtgcgg
    26641 aatgtcgctg taaaaactat gaaaatgtac aaagtggaaa attaagtggg aaaaaccgca
    26701 atttagcaag taccctttat atcggttcaa aggcaagcac aaaatatttc tgcctgtatg
    26761 aaaaacaaaa ggaacaggca acgaaaaaga aacataccga tattatcaac cgttttgaaa
    26821 ttcgtctgcg tgataaaaag gcagtacagg cagttgagga attgttattg acatataacc
    26881 ctcatgggct ggtgttttac ctcattactg attttgtgca atttcccgac tatccgttgt
    26941 gggagatatt catttcccat gacagtttac cttttgaaat gaaccccgta cctgtcaata
    27001 tggaacgcac tctgcaatgg ctggaaagac aggtcatgcc gtcaatcgtg atgatagagg
    27061 aaatcgacag gctgacaggc tcaaactaca tgaaaatgat tgatgaatgt acccaccttt
    27121 ccgaaaaaca ggaaatgctt gtagaacaga tgtgtacgga tatagcagat gtaatcgaaa
    27181 gtgagggggt gttttatgag taatgcacag gatattcccg tatgggaaaa atataccctt
    27241 accattgaag aagcgtcaaa gtatttccgt atcggagaaa acaagttaag acgattggca
    27301 gaggaaaaca aggacgctgg ctggctcatt atgaatggca accgcataca gattaaacgc
    27361 cgacagtttg aacaggttat tgataaattg gacgcaatct aatgcaaatg agccttgtat
    27421 gtgttatgat aagcacaagt catatcaagg ctctttccaa caaggaaagg agcagacacc
    27481 atgagagaaa aaagacggga taacaaaggt cgtatcctgc atactggaga gagccaacga
    27541 acagacggaa aatacttata taaatatgta gacgcatttg gaaacacaaa atatgtgtat
    27601 gcttggagat tgacacccac agacccgaca ccaaagggaa aacggaaaaa accctcactt
    27661 cgagaactgg aacagcagat aagacgggat attgaggacg gtatcgacag cgcaggcaag
    27721 aaaatgacgc tttgccaact ctatgccaaa cagaacgcac aaagggcaaa tgtgaagaaa
    27781 agcacacaaa aacaacggga acaactcatg cggttattga aagaggacaa attaggtgct
    27841 aggagcattg atacaatcaa gccctctgac gctaaagaat gggcgttacg catgaaagac
    27901 aagggctttt cctacaatac cattaacaac cataaacgct cgttaaaagc gtcattctat
    27961 atcgccatac aagacgattg cgtaaggaaa aatccctttg atttcaaatt aagtgaagtc
    28021 ctagaaaatg ataccaaaga gaaagtcgca ttgacagagg aacaggaaca agccttactc
    28081 tcattcatca agacggacaa tgtgtatcac aaatattatg atgatatgct gatattgtta
    28141 aaaacaggac ttcgtatctc ggaactgtgc ggactgacag tagccgatat tgatttcaag
    28201 aatgaagttg tgattatcga ccaccagtta ttaaagaaca aggaacaggg ctattatatt
    28261 gaaacgccta aaacaaagag cggaataagg caagtgccat taagcagaga aacgatacag
    28321 gcatttcaac gggttatgaa aaaacgccca aaggcagaac catttgtgat agacggacgg
    28381 agcaattttc tgtttgtcaa tcataagggt aggcccaaag ttgcgattga ttacaacgcc
    28441 ttatttgtcc gaatggtaaa gaaatacaac aagcaccaca aggacaatcc cttgccacat
    28501 atcacaccgc atacgctacg ccatacattc tgcacaaggc tggcaagcag gaacatgaac
    28561 ccgaaagatt tacagtatat catgggacat tcaaacatca gtatcacaat gaactggtat
    28621 gctcatgcgt ccatagatac cgcaaaatca gaggttcagc gtctaatcgc ataagaagta
    28681 tttaccacga ttttaaccac gattgacagg gaaaatataa gaagatagat ctagatatgt
    28741 gaggtttacc acaaaaacaa aatgcccgta gagcctataa aataaggctt tgcggacatt
    28801 taagaagata taaaaagata gtcaaaaaga catatataat ttattctaaa aaaacaacaa
    28861 tttttcgttc tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa
    28921 agcaaacaat aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag
    28981 gtggagaaaa tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg
    29041 attggtccaa gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt
    29101 atatttggcg aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc
    29161 tatcgggggc acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat
    29221 gatgaacgtt tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt
    29281 gttcctaaga gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga
    29341 acacgaaagc tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa
    29401 ttgaatggat tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa
    29461 gatgttccag gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc
    29521 ggcactatga cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta
    29581 gatgatcctc aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc
    29641 gtaaattatt tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat
    29701 caagcaaaag cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt
    29761 ggccgttccc gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa
    29821 tcaatcaacg agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca
    29881 aaaaaattag atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa
    29941 gctgtacgca atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct
    30001 acaccgactg ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg
    30061 cttgatttga cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta
    30121 gtcatcgcaa acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt
    30181 tctgcaagtg caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa
    30241 gcagcacaag cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt
    30301 gcaggcttag ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt
    30361 atctctgctg atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt
    30421 gctgcaatgt accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt
    30481 ggcttagctg ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg
    30541 tgataaagct ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa
    30601 gagaggacta attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac
    30661 ttcatcgatc gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg
    30721 ttcaaaaaca gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg
    30781 gccaatcgca actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg
    30841 gacaccagag tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa
    30901 tacttcaaag gacgcaggac ctccatctag ttgacgtcca ccatgattgg atacccagat
    30961 accatctgcc cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac
    31021 gataactgga agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg
    31081 ttttgcagct gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg
    31141 ttcgctatac gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc
    31201 ttcttcacgg taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc
    31261 ttctactgct ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg
    31321 gaaaaactgc ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata
    31381 agtgctgatg gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc
    31441 tttttcatgt gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc
    31501 aattccgaaa acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat
    31561 gattttcttt gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc
    31621 accgcgaata tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta
    31681 ggcaatgttt acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga
    31741 tccttctttc atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa
    31801 catttactct cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat
    31861 ctacttcttg gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt
    31921 cctctatttc atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg
    31981 gtactgttcc atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag
    32041 tggttatagg aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag
    32101 caggcccctt tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa
    32161 aaggcgcgcc aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg
    32221 ttcctaaata gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat
    32281 ctatgccgta gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc
    32341 actcgttatt tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag
    32401 acaaatctcc tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta
    32461 ctgcttgctg atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc
    32521 tgtagccgtg gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg
    32581 ccgttttttc tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta
    32641 acgcaaacaa actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga
    32701 ttggttctgt catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa
    32761 tcatatgatc gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca
    32821 agttctgtac tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct
    32881 ccgtcaactt ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg
    32941 acttcgccaa cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag
    33001 atatgattga cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca
    33061 acgaatcctg tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc
    33121 atttcaacaa gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt
    33181 ttgacttcag tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc
    33241 atactttgcg cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta
    33301 tgcaagacat accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa
    33361 ataaaaaaac ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac
    33421 gttgtggcta gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat
    33481 tacgtaaaac atcaatccga aaataataga agtttcgatg actactaacg tatctttacg
    33541 gagttgtttt ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa
    33601 tttcatgaga caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga
    33661 ttacaatatt tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact
    33721 gattttgtgt agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc
    33781 atttacttct gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact
    33841 ttttacttgt caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat
    33901 taatttgaaa ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact
    33961 tgtatataag aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt
    34021 aaaaatttta gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat
    34081 tttccgtgga aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat
    34141 gacgcttcct gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc
    34201 tttcttcaaa gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga
    34261 aaatcatttt tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg
    34321 gagcgtagaa caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt
    34381 ggctgctatt gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga
    34441 tatcgaaaaa gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc
    34501 gaaagtatgt gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat
    34561 tgaaaatttg caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg
    34621 ctttcttttt tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa
    34681 taatacatca atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca
    34741 taggttgtcc aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg
    34801 ctgatggtaa agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt
    34861 tgcaataaag ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc
    34921 atgataccaa aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc
    34981 aatttacggt taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc
    35041 cctgaggata caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca
    35101 tttacaacga ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt
    35161 ttctcctagt ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat
    35221 aattataaca gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag
    35281 cttaaacaat gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg
    35341 gtcataagaa ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga
    35401 aacttaaata aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct
    35461 agcaagcagc tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt
    35521 aatggatcat gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt
    35581 tcctagactt ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac
    35641 aagtttcttt cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag
    35701 gagtattaaa atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca
    35761 tatattgatc aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt
    35821 accggtatta aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca
    35881 cgatgctgag attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga
    35941 acagaaaaca atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca
    36001 tcaggtactt gatactttct atcagatgga agtagaattc ccagttgctt atattccagc
    36061 aggttctgga gatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact
    36121 tcagctgatt ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa
    36181 aataagtgaa aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat
    36241 cgtccatgcg acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc
    36301 attttcgtat ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct
    36361 ggtagatgca ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa
    36421 gcatcctttt tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt
    36481 cattgatttt gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt
    36541 acttatacag aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt
    36601 acgtatcgtt tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg
    36661 accttttgat atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt
    36721 ttgtcagaag ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt
    36781 tgtcacagtc ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata
    36841 gtttctttct ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa
    36901 gacacaacca ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact
    36961 gatcaagtta ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac
    37021 catttctggc aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat
    37081 aaaaatatct ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc
    37141 cgaaaagcta atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt
    37201 tatatcccac gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa
    37261 cagcagcgag taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat
    37321 gggcgcactc tggtttcgtt tactgatctc tttgcgatta aatgctagat tgatgacgct
    37381 gaaaccgcta aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt
    37441 caacagcttt ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga
    37501 gagtgccatt tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag
    37561 aatcagtgtt tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc
    37621 tgggcgactc tgtatcttga tcgtcggcat ctttcctact tcgacttgtt cggtaaagtc
    37681 tgcaggcaga tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt
    37741 ttgtgaggtt ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc
    37801 atcgactaca ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt
    37861 attttcaaca tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc
    37921 tatcagtaaa ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat
    37981 aacgcacccc tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca
    38041 accatagaag attggtataa aaactagcca aatcagtgta gaaataaagt tt
//
LOCUS       DAJSAW010000025        37691 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJSAW010000025 DAJSAW010000000
VERSION     DAJSAW010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.025"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(97..1041)
                     /locus_tag="ONT64_001397"
     CDS             complement(97..1041)
                     /locus_tag="ONT64_001397"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCR2302821.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1337..2281)
                     /locus_tag="ONT64_001398"
     CDS             complement(1337..2281)
                     /locus_tag="ONT64_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2302822.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2502..3716
                     /locus_tag="ONT64_001399"
     CDS             2502..3716
                     /locus_tag="ONT64_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2302823.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3738..5753
                     /locus_tag="ONT64_001400"
     CDS             3738..5753
                     /locus_tag="ONT64_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HCR2302824.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5836..7209)
                     /locus_tag="ONT64_001401"
     CDS             complement(5836..7209)
                     /locus_tag="ONT64_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR2302825.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7235..7519)
                     /locus_tag="ONT64_001402"
     CDS             complement(7235..7519)
                     /locus_tag="ONT64_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2302826.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7521..9536)
                     /locus_tag="ONT64_001403"
     CDS             complement(7521..9536)
                     /locus_tag="ONT64_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR2302827.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9715..10020)
                     /locus_tag="ONT64_001404"
     CDS             complement(9715..10020)
                     /locus_tag="ONT64_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HCR2302828.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10109..11140)
                     /locus_tag="ONT64_001405"
     CDS             complement(10109..11140)
                     /locus_tag="ONT64_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2302829.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11347..12222
                     /locus_tag="ONT64_001406"
     CDS             11347..12222
                     /locus_tag="ONT64_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2302830.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12374..13216)
                     /locus_tag="ONT64_001407"
     CDS             complement(12374..13216)
                     /locus_tag="ONT64_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR2302831.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13308..15185)
                     /locus_tag="ONT64_001408"
     CDS             complement(13308..15185)
                     /locus_tag="ONT64_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR2302832.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15182..16603)
                     /locus_tag="ONT64_001409"
     CDS             complement(15182..16603)
                     /locus_tag="ONT64_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR2302833.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16606..19995)
                     /locus_tag="ONT64_001410"
     CDS             complement(16606..19995)
                     /locus_tag="ONT64_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR2302834.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20252..21643
                     /locus_tag="ONT64_001411"
     CDS             20252..21643
                     /locus_tag="ONT64_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2302835.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21716..22363)
                     /locus_tag="ONT64_001412"
     CDS             complement(21716..22363)
                     /locus_tag="ONT64_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR2302836.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22371..23057)
                     /locus_tag="ONT64_001413"
     CDS             complement(22371..23057)
                     /locus_tag="ONT64_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2302837.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23063..24136)
                     /locus_tag="ONT64_001414"
     CDS             complement(23063..24136)
                     /locus_tag="ONT64_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2302838.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24358..25032
                     /locus_tag="ONT64_001415"
     CDS             24358..25032
                     /locus_tag="ONT64_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2302839.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25037..26377
                     /locus_tag="ONT64_001416"
     CDS             25037..26377
                     /locus_tag="ONT64_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2302840.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26526..27512)
                     /locus_tag="ONT64_001417"
     CDS             complement(26526..27512)
                     /locus_tag="ONT64_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HCR2302841.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28236..29267
                     /locus_tag="ONT64_001418"
     CDS             28236..29267
                     /locus_tag="ONT64_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2302842.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29376..30908
                     /locus_tag="ONT64_001419"
     CDS             29376..30908
                     /locus_tag="ONT64_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HCR2302843.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            31047..31859
                     /locus_tag="ONT64_001420"
     CDS             31047..31859
                     /locus_tag="ONT64_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HCR2302844.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            31849..32334
                     /locus_tag="ONT64_001421"
     CDS             31849..32334
                     /locus_tag="ONT64_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR2302845.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            32357..32902
                     /locus_tag="ONT64_001422"
     CDS             32357..32902
                     /locus_tag="ONT64_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCR2302846.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(32975..34618)
                     /locus_tag="ONT64_001423"
     CDS             complement(32975..34618)
                     /locus_tag="ONT64_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR2302847.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(34747..36975)
                     /locus_tag="ONT64_001424"
     CDS             complement(34747..36975)
                     /locus_tag="ONT64_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2302848.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(37008..37487)
                     /locus_tag="ONT64_001425"
     CDS             complement(37008..37487)
                     /locus_tag="ONT64_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302849.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        10979 a         7150 c         6872 g        12690 t
ORIGIN      
        1 ttatttgctt acctattaga tatcaataaa aaactgctta acagctattt cagctgttaa
       61 gcagttttag gatttttagt tcatattcat tgtttcttat aatacagtgt tcaatacttc
      121 tcttaaagca cttgttgatt tgctgaacat tgctttttct tcttcgttga ttgataattc
      181 aacaacttca cgaacaccat tttcgtcaac gattgatggc acacctgtaa agatatcttt
      241 ttcgtcgtat tcaccagtta aataagcaga tacaggcaat acagcttgtt cgttattcaa
      301 gatagctttt acgatacgtg cagtactcat accaatacca tagtaagttg cttttttacg
      361 atcaatgatc tcataagcag cattacggac tttatcagca atcacatcta attcatcttg
      421 ggcaatacga tggtcttttt ctacaatttc aaacactggt ttaccaccga cagttgtatg
      481 tgaccatgca gcaacttctg aatcaccatg ttcacctaag atatagccgt ggacgctgcg
      541 tgggtcaact tttaatttca atgcgatttc tttacggaaa cgagttgtat ccaatgttgt
      601 tccagtaccg ataacacgtg atgttggcag accagattcg ttccaagcga tatatgtcaa
      661 aatatctact gggtttgaag caacaacgat aataccgtcg aatcctgatc ccatgatttc
      721 ttttacgatt tggcgcatga ttgacgcatt tgttttaact aaatctaaac gagattgacc
      781 aggtttttgg ttgataccag ctgtgataac aacgatatca gcatctttac attcttcata
      841 gccacctgac catacagcca cattttcatc gccccaagcc ataccatcta aaaggtccaa
      901 tgcttcccct tctgcttttt cttgattcac atcgatcaat actaattcat tagagattcc
      961 ttggttgatc attgcataag cgatacttgt tccaacaaaa cctgtaccga cgattactac
     1021 tttacgactt gtttttttca tgaataaaca gctccttttc ataactattt gttactttca
     1081 tgaaaagtat agtcttgttt attcatttta tcaacatgtt aattataata gttgttatta
     1141 taatttttga tggaaacgaa ttcctgaata cgcataacag ataaatgaca aatgtttaca
     1201 cctatatccc ctgataatac aaccaaaagt ttagaactga aaataaatcc aaaaaaattc
     1261 gctgcaacga tataataaga cagcaatcgc acctaaaaca attttccttc atatgcttta
     1321 gtgaaagatt ttcttcttaa tgtttatttc tccattcatt gggagtacat gttgaatact
     1381 tcttgaataa tttgaataaa gaagtcgttt gtttatatcc gatacgatta gcgatttctt
     1441 ccaacgaaaa actcgtttct tgaatcagtt ccaacgcaaa gtcgaatttt ttctttagaa
     1501 ccagttcttg aaaggaagca tcaaaataca ttttcaattt attactaaga tagaccggat
     1561 tgtagccaaa atgagctgct gtttctttta acgtacaaga agtaaaatgc ttatcgatat
     1621 aatctaatac tctaagcatc tcattttctg cgcatccatc tgctgatgaa aagctttgtt
     1681 gttctaataa atgagtcaat tcgatcagta atagtgatgt atagagattt aacgaacgga
     1741 tcttatgact gtatgtatca aaataaatca acagcatatt ttcaatcaaa tgcgtcaaac
     1801 gacttgtttt tccgccgcga aaatgaatga attgtgtatg ttcccctttt tcttttgatg
     1861 catttatcaa aaaagaagaa agtagattat ttgtgcttgc caaatgattc aatatctctg
     1921 tagataaggt ttcttctttt aataagatgt tgattaaaat atcctcttgc cctgtgtatt
     1981 cgattctttg agtagcttct ttatcaatga tcaccacgtc actttcttcc atctctatcc
     2041 tttctccatt aataatttgt aaacatgtac cgctatacat ataattgatt tcaacaaaat
     2101 catgtgtatg ctcgggaact tccgcatatc gacggtgttt actgatatga atatttccct
     2161 gttgaaatag atcccctcgg tggatttttg gcggtgtctg aagatggtaa gctaagttta
     2221 aatcattgat attcattcct tgttcacgct gcagagtttc gattgattct tcgttacgca
     2281 aaaaaaatgt ctaattcagc tagttccaat tgactccctc ctttttgtaa gcggtttctt
     2341 tcgcttatta gttcacgctg tactgctatt atatgcaaag ctatttttgg tttcataaag
     2401 actttctatg gttgggacat gaacttcctg tgattccgct attgagagaa taaccacttc
     2461 atactatatt tttgataagc gcttacaaaa atgaaaggaa catgcttatg aataaaaaga
     2521 tgatgaaatt ttctctgctg tcagtctctc ttattttgac ttctgctggt gcagtcacgt
     2581 ctgcaattcc cagtatgtca gctacttatg ccacgcgttc tttatctgct gttgaattat
     2641 taacgactgc tccctctttt atggtcacta tttttgtttt gattagtagt tttattgcta
     2701 ggaaaatcgg acaaaaacaa actgtgatca tcggattagt gattgcatcg atttgtggta
     2761 tcctccccct cttctcacaa agttacagta ttgtcttagc ttcaagagct ggactaggta
     2821 ttggctttgg attgatcaat tctttggcag tcagcatgat tgctgccttt tatcacggag
     2881 atgaacgagc ctctatgatt ggttttcaaa gtgcatttca aggatttggt gcagctttaa
     2941 tgacatttgt tgcaggacag ctagtacagt tcggttggca atataccttt ttagtctacg
     3001 ccatcagctt acctattctt gtcttgtttg ttttgtttgt tcctacacct gaaacaactg
     3061 aacttcaatc gcatggcaca gccccaacaa gacaagtaat caaaacgaat cctaaaatga
     3121 ttagttatgg tatttttctc ttgctggtca tcgtggtata taatgctgta tcgattaaat
     3181 tagccacagt cctgacgact tattctctag gaaacgaaac taatgcagcg aatatcttaa
     3241 caatcatgca attggcaagt atggctgccg gagtttgttt tggctggctt cagacaaaag
     3301 cacatcgcta tatgcttaca ctttctttac tactgatggg aatcggcttt gtattgattg
     3361 ccactgtgat aaacctgtat gtgattgggt ttggtgcgat cctaacagga atttcttttt
     3421 cgttattcat tccttactta ttcaaccaag taagtatcca agcgcccaag tacgcggaga
     3481 gtttcaatac ttcaatctta ttggttggcg ctaatttagg tactttcatt gcaccatatg
     3541 gtttgctctt tctctctttt gcttctcttg ggagtaaaac acttgctgtt ttcgtcaatg
     3601 gcagtatctt gctgttgatt agcgcgctta ttagccttct agtagtacac aggacgaata
     3661 gaaagacagt gataagagta gatgaagaaa taaaagaaca attgacaaca aaataaattt
     3721 gatggaggtc gtgtaaaatg acaaatgaaa aacataaaga tagatggctt tggtatcctg
     3781 gagactttga gatccgccat ggactgctac aaaatttcca acgggaagaa cgaggttttg
     3841 actggccagc gtactggtac atggatgatt gccatcggaa cgtcaagttt aagagatatt
     3901 actttcttga tcaaccttcc atgtttaaag tgacgatcca aggagttggg tatgtagaaa
     3961 ttaatggaca aaaacatcca tgtggaaaat ggctgacttg tccagctggt aaagcaaaaa
     4021 ttcgtatctt tgtaggacat acatcagggc taccagcgat gtttatcgag ggagacgaag
     4081 taaaatcaga tattggctgg acagcaagta attttattga agaatatcca gctggctgga
     4141 gtccgcttta tgtcgatatc gcaaaagatc caaatcaaat ctattatcaa aaagaatgcg
     4201 tccatccagt cactgaaacg gagattggcg gaggaatctt attcgatttt gggcgagcag
     4261 tgaatggctg tgtagaacta atatctctgg atgaccatat cgaatctatc actctatgtt
     4321 atggagagtc cgatgtagaa gctatggata tcgattattg ctactataag caaataggaa
     4381 tgacaagaaa aatgaaagct cgtaaacgcg cttttcgcta tttgtttata ccagaggttt
     4441 ctactggaat ggttcaactt caagtttttc atgaattttt atctctttcg aaacaagctg
     4501 ctttttcaag cgattcttct ttactgaatc agatctggtc agtatcagaa gaagccttca
     4561 agttatgtag tgatttgttt tttatcgacg gtatcaaacg agatcgctgg atttggtctg
     4621 gagatgcgta tcaatcattc atgatcaatc aatatctttt ttttgacgag gaaatcaata
     4681 aacgaacgat attagcatta agaggacaaa atgaaatcaa gcagcatatg aatactatcg
     4741 ttgactattc gatcttgtgg ttgattggta tcgaaaacca ctatatgatg acgaaagact
     4801 atgaattttt acgcatcgtc taccctaaga tgcaatcatt aatgagatac ttgatggagc
     4861 aaacgaatga attagggttt atttatggga gagcaaaaga ttggatattt attgattggt
     4921 ctgaaatgga taaagaagga acgcttgctg cagaacaagt cttattactg aaagcctatc
     4981 attccatgat cacttgcgga gatatcttag ctgaaacata tggagagaac gaaaattatc
     5041 atactcatct ctatcaagaa aaatatcaac tattgaaaca aaacctattt gactactttt
     5101 gggatgaaga acttggcgct tttatcgatt gctatgaatc tgggagaaga aatgtgacgc
     5161 gtcatgcgaa tattttcgct gttttattcg attttgctaa caaagaccaa cagcaatcga
     5221 tcttaaagaa tgtcttattg aatgacacta tcacacagat tactactcca tatttcaaat
     5281 tctatgaaca agatgcttta tgcaaactcg gacagacgaa tatcgtctac caaacgattc
     5341 ttgattattg gggagggatg ttatctcatg atgcggtaac tttctgggaa gaatacgacc
     5401 ctactcaaac aggaaatgaa aagtacagta tgtatggaga tccttatgga aaaagcttat
     5461 gccatgcatg gggagcaagc ccaatctatc ttttaggacg ctattttatc ggtttgtatc
     5521 caacaaagcc aggttatgaa gaatttatga tacaacctaa tctagattca tttgaaacac
     5581 tgaattgtac gctacctatc aaagatggaa agatttcgct tgaaaaaaca aaagataaga
     5641 tcgtgattac ttctgataga acaggcggaa cagtgaaaat gaatggtaaa agttatcctc
     5701 ttctagcaaa acagccactt gtcttaacaa gctattcaaa aataaatgca taaaacaaaa
     5761 aactgcttgc gacatatcag aagactttaa atcttcttct gatattgtcc aagcaatttt
     5821 tattttgaag tttgcttaat tctgttcttt agaaacaatg tttgttcttt ttccggcaaa
     5881 aagaaagcta gataatacga caagtaaaaa gaaagcgatg attccaactg tcaaggctat
     5941 ttgaccgtat tctcctagtt tcccaagtac aagtcctggt actagataat cagtatcggc
     6001 aaatgtagaa gaagcgattt taagatctcc taatactgga aggataaata atggcagcca
     6061 actaatcaat aagctattga aagcagaacc taataccgcc cctcttctac cgccagtcga
     6121 gttgccgaag acaccagaag cagctccgca gaagaaatgt cctactaccc caggaatgat
     6181 aactgtagtt tgcaagccta tcatagcaaa catggatagt gttcctacaa agaagctaac
     6241 aaaaaagcca attaatacag cattaggtgc atatggaaaa acgataggaa catctaatgc
     6301 tggtttagaa tcgggtacga gttttttagc aatcccttgg aatgctggaa cgatttctgc
     6361 caacaccatt cgaacccctt gaaggacgat aacaaaccca gctgtgaatg ttccagcttg
     6421 gatcagtgca aagattacag cgttgtctcc attgcttagt tctctttcaa caaatccagg
     6481 accagctaac aaagcaagtg cgacatatac gatcgacatc aagagcatga tcgatacagt
     6541 agaatctcgc aaaaagctga aacttttagg aatctttatg tcctctgtag aacggtcttt
     6601 gtttccaaaa aactttccga tccatccact agaagcataa ccgatatttc cggtatgccc
     6661 taacgcaact tgattgtttc cagtgatgtt cttcataaac ggttgacaaa gagcaggcgt
     6721 aatagttaat agtgttcctt cgaagacccc gcctaataag atcatccaca aatttgcagt
     6781 aaaccccgcg cttactaaaa tgactgctgt tagacaagaa acataaagca ttgcttgacc
     6841 tgtaagaaaa atgaatttat atcttgtgaa tctagcaagt aaaatattca cgatcatccc
     6901 tactagcatg atcaaagcag taggtgtacc gtattgttgc aaagcaatcg ccactactgc
     6961 ttcattgcta ggaactactc cttgagtatg cagagctgtt tggaacatcg aagcaaaagg
     7021 ggttagtgaa ttactgatta aaccagcacc gccgatcagt actaggaatc cgacaaatgt
     7081 tttgatagtt ccttggatca cttctgaaaa tttcttcttc tgaagaacga gaccaagcag
     7141 tgcaatcaaa gaaacgagga tggagggctg actgacgatt gacacaaaca aattcaacaa
     7201 tttatccatt gatagttcct cccttgaatc atttttattc ttttgacaag atcttcgtta
     7261 ctttttcttt tacttcttcg ctgtcaatga tcgattgcaa aggtacgagc ttttcatcag
     7321 gtatagaagt tagcgtatct ttcaaagaat gctcaatgaa gaaataatca gccatatcgc
     7381 tagatacact tccaacatcc gtatgactta cttcaaactc gtccacatct gctccaagat
     7441 ctgcaagtgc ttgtttgatg ttcatctcta ccataaaact tgttcctaat ccactttgac
     7501 atactgcaaa aattttcata tcattcgtct ccttttctta tgatcgctaa tatttcttct
     7561 tttgttgatg cgtgtaataa atcttgaaga ttttgtttat tagaaagtag ctttgttaaa
     7621 tttgccaaag cccgaagatg tgcctcatta tcgatagcag ctaaacagat gaacaaacta
     7681 atgggatggg ctggattatc aagcagatcc acaggttgtt gaagtttcag caaagacatc
     7741 cctacttcgt ttacgccatc ttctggacga gcatgaggta atgcgatgtg atcgccaata
     7801 tggataaacg caccatactg ctccacgcgc tcgatcatag ctttgatata actttcttct
     7861 attttatttt ctgataatag cggctgtgcg gcaaatgtga ttgcttcttg ccagtttttt
     7921 ggttgatccg ataattggat catttcttct gttaataatt cagatagcat aggacgttga
     7981 tcctccttct tttctaaaag tttgttcatt ttacgattta atacttgata gagcttttct
     8041 tcgtctacgc cttcttttaa ttcgatataa ggcagtagcg ccgacaacaa ttcttgggca
     8101 gaaggcattg aaaatcctgg tatcagccaa tctctttgta cttgattgat cagctgattt
     8161 ttttccaagt cagtcatgat tggatgcaga acatagagag actttttctt atcagtaagt
     8221 gtgagaggta cagtagaaaa aataatatcg taagaagttt cgtcaacctc gtgtaagcgg
     8281 tcagctgaat tagtctctgt aaaaaagatc atcgggaata attgctgaag ttcacttttt
     8341 aagatcaatg aagagctaat gccacttgga cagacgatca cggctcttat ttttctattt
     8401 ctttcttctg cgtccgcttt tctgatctct ccgccaaata gtatagtgaa aaaaccaatc
     8461 tcttcttcag ggatgctctt ccctattcgt ctttctaacg gaagtaatgc ttttcttgtc
     8521 atttcgtaga tggaagcata ttgatgctta atttgatcga tcatcacatt aggtaaataa
     8581 aaagaatagg aaatacgaaa ataggcaggg accaagtgat aaaagacatt catcaataat
     8641 tcacgaaacg aagcaaattc tacggccatc aggaattcca tgcgatggat gacttctgaa
     8701 gaacattcca gcaaaaaatc aagtgcaggc tcctggacat ctccttcaac gatcgatgct
     8761 aagcaaagag agaaaaatat aatctccgaa ttttgatgtt cggaatagcc aaggccagtc
     8821 aatacttgtc gactaatgtc agtgactgca agagaagcca acagttcagc atcttttacc
     8881 agttccatgg atgtttttcc aattcgaatg gttgtcaaac tcaaataata agctaattca
     8941 tccattcgat ttggtgcgaa tcgaagatct gatagtgaaa tcgttttatc tagcaaatct
     9001 ctaactttgg cgtaataatc aatggaaagg gttgcggtta aatacgtgag cccaaatctt
     9061 ccagcaagtg actgcatcaa acgagcagtg cgattttttg cttctcttct taatattgct
     9121 tctgacccta gcagcctaaa accagttttc ctagtatacg acagctgaca atcttccact
     9181 tctaattcat ttcttatctt tttgatatct gacaggactg tccctttaga aacctgaaga
     9241 aaatcttgaa aatgaaaaac agaaacagac gttgcagtcg aataaaccat caaataaaga
     9301 agatctcttc gctcttgttc ggtatacaca gcttcaaatc gagtaatatc caaaaatttt
     9361 tctgcccagt cgattgctcg gaatttcttt atacggatac gggtatcatc cacatattcg
     9421 gcaaaaggag ttaattgttt gatcgcctcg cttgtattta cttcgatgat ctctgctgct
     9481 ttttctagac tgatttctct tggtgttttc aggagataac taattttgta gttcacagcg
     9541 ctgttcacct cctacttata ttctagtatg gtagcgcttt taaatcgagg ggtgtttggt
     9601 acaacttaca gttggatttt ttttacgact gtaccgaacg atttctatcg aatggataca
     9661 gaaaaaaagc aggtgaccaa atgtctcctg cttttatgaa ttgatttcgt ccgatcaatg
     9721 atgatcatgt ttattttttg aggtcagttc ttcaaatttt tctttgacat cttcaaccgc
     9781 tttttcgata tctttttcaa agtgattggg ctcattaggc tttgtttcct cttctacaga
     9841 atttgaacca gataaagctg tttctttatt ttctgtttca ccagagtttg tgccagctaa
     9901 tgattcttct ttgtcttctg tttcaccaga attagttcct gctaaagcca cttcttcttc
     9961 tgttttaggg gccattgaac ctcttaatcg tttgtgtttt tcaccgtcat aatttgtcat
    10021 gttggttcct cctagtagtt ctatactttt tcatcgtaca ataagacagt ttatgcgtca
    10081 aaaaacaaac ccgattctta caaatatgtc atatttttcc ttctgattct tgaatgcttt
    10141 tgattccttc ttccaaaatt ttcatttgta agattgtttc ttcatctttg atgacttttt
    10201 ctgcgccatt aactaatgtc tcgtaaatcc cttcgtagac acggctgtaa tcaccaacct
    10261 cggaaattac tttttcttca tgataactcc cttgttcatc tatataggtc aatacaccat
    10321 aatgttcggg caaatcgata ccaaagtctt catgatcagg catgtaaaac attttcagat
    10381 gttcttcttg tcgatctttt gtttgtttca cgaacatccc tttttttccg taaacgacga
    10441 aacttggacg ttctttgatg cggaaataac tggattttat cgaaattttc atatttccat
    10501 agaaaaaatc cagatcgaag taatcattca ttcgtccttt ccctaaaagt tgacgtacat
    10561 catagctgat atcatctggt tcgccgaaat aagaaagaac ttgatctact gtatgacaag
    10621 catgtccata taaatagctg ttcgcagtag aaaattcatg cgtattttct ggtacttccg
    10681 gtctgaaata atcataatgc atttctactt ctaaaatatc ccctaatttt ccgctttcga
    10741 ttactttttg gactgtcaag aagtcagaat cgaaccgtct gttttggtaa cattggataa
    10801 atagaccttt ttcttttgct aatgcaaata actcttctgc ttcctttgac gtttctgcaa
    10861 aaggtttctc tactagtaca ttcttaccat gttcgagtgc catttttcca tattcataat
    10921 gaaattgact aggtgtagtg atgacaacta attggatctc aggatcatcc caaagatcag
    10981 acagctgatc agtatattcg atgtcttcga atgttttcca cactggttca gatggggaaa
    11041 aaattttttt gatattgatt ttgttcgtaa gttttcttga aaaaggcaaa tgatagcgat
    11101 ttgtactctt tccattgcca agataagcga ttgtaagcat gagtggtgcc tccttttatt
    11161 gatgttttca gtatatccta gaaaagcgca gaaagtattt ataatagcta ttctggtaaa
    11221 aaagttcaga aaaacacttg ttatcattcg gtttggtgaa attaatgttc aaagtaagga
    11281 taaaaatcaa aaagatagta gcggaaccaa gtgtttatca gtaagataga atttggaggt
    11341 cagatcatgc ttattgaaga aaaactaaca aaacaagagt tgtttaccac gacagagaaa
    11401 cggattgtcg attacatccg taggaatatc gaagctgcag tctacatgac gattgaagaa
    11461 ttagcaaaag ctacgtatac ttcccattca gctatcattc gtttatgcaa aaaaatggga
    11521 ttcagcggat ttaaagaatt ccgtttcgag ctggggcggg aagttcatca attgattgct
    11581 cagttcaatc aaacgattga tcctaatttt cctttttcaa gtgaagatag cactcagcaa
    11641 attgcaaaga aaatggcgga tttatcgatc cagtcaataa aaaaagcaca attgcagatt
    11701 gaacatgaag atttagatca aatcggtaaa gtattgaaca aggcacagcg aatctttctt
    11761 tttgcaaaag gtgattctca aatcacggca aggaaattcc agaataaaat ggtcaagttg
    11821 aataagtttt tgatcatggc tgaagaatac agtgattctt cttggaatgc agccaatttg
    11881 actagcgagg attgcgccat ttttattagc tacagcggtc gcatccatca ttatgaacga
    11941 ataatgacct atttgaagca tgtagaggcc cctaccttgc tgattaccgg aaatcagcat
    12001 tcagaaatgg ccaaacaggc tgatatgtgt cttgttatat cgcaggagga atatgatttt
    12061 gctaaagtag cgactttctc ctctcaaatt gccttcgatt acgtattaaa tacgttattc
    12121 tcagtgatat attctcaaaa ttttgaagag aatatccgca acttgaaaaa caaacaagaa
    12181 ttgatgcaaa aaggccttct taaagaatat cggaaaactt aagaaaagat cctcatttag
    12241 taataagaaa gatagtgttt gataggctct ctttgatatc atcataaaaa caggctgaag
    12301 ttttcttttt caaacctcag cctgtttgtt tacggaacga ttcgaataga tatattatag
    12361 aaatgaggaa tagctaactt ctgtttctta ttttgaacga ttgacgaaaa agatatataa
    12421 gaaggaggaa aatcagtata catgcagcta acaatccata caggaaaaat cgtttgttct
    12481 cggatacttt ctgtacttct ttttgaactt cttttgcatg ataaggaacc cggtgccctc
    12541 tgacaagtaa gcgatgactg ttgaccatat aaggtgtaca agttaggagt gtcacgaaat
    12601 cctgcccttt ttcgattctc agatcttttg tatcagtcgg ttctaccgtc ttgatctgat
    12661 cgacttgata agcgagtgtc cgtccattga tttcgatata gaaagaatca cctaaagtca
    12721 gtttcggtaa gtctgtgaac aactttgctt gaggcagtcc tcgatggctg gaaataacgg
    12781 catgtgtatt ttctcccccc gtaggataag acgttccttc caataatgag gcaccttttt
    12841 ctagcagcac agtattcgtt tcatcaaaaa ttggcaaatg gacattgatc tcagggatcg
    12901 tcaaaatacc gatcgtatgt ttctcaaaat aggttctatc ctttttcgca ggttcttttt
    12961 tcttagtaaa gggatctgca cctggatttc caccttcttt tgctaattgc tggtttttct
    13021 ttgtcatgtt ttcttggatt ttagccatga cttcttcatt ttctttgacc gcttgttgct
    13081 gataatgaga gatgatctgc tgatccagat aattattcag cgcgtcgcta acaaatggat
    13141 aagctagtat ccctattcct gagagaagca agaaaagcat cagcagatct agaagtttcc
    13201 ttttctttgg tttcatttgt gttctccttg tgatgactgt ttgataataa aagaaaaagg
    13261 agcatttgcg taatccgctg ctcctttttc caattcttgc aaaaagacta aatctgactg
    13321 tgcttgcgtc tagcaaagta cagtccagca atcagtagaa ggactactcc tgcaccgata
    13381 tacacataga ttcccttacc gcctgttgaa ggaagtgtac ctttgtgttt atttggtatt
    13441 ttttcaggag aaatcaactg tcctgctgtt acataagatt gttcatcgat agtaaatgct
    13501 acacggtttg ttaatggaac atatttttct ggcgctttcg tttcttccag atagtacgtg
    13561 ccatatttta gacctgtcac atcgattaaa ccgttacttg tggttgtaaa aaccgttgct
    13621 gattctttcg ccgataccca gctgacggct tttgtggatg ggtcgatcga taaatatttc
    13681 gctgtgtcac tatcttgatc acgaacgacg aattctgctc cagcaagtgt ttggtctgat
    13741 gtaacgtcac catctacttt aacaaatcgt ttgcccccag taacgacatc gactgacggt
    13801 ggtgtttgat catttgtatg cccgttatcg acatttgctt ggttagaaaa tcctttggtt
    13861 ggatctgctt tttcgttcaa atgcatatag taaacgaatt tcaatgtacc gccaggagtt
    13921 aatgaaggaa tataattcgg atcaactgaa accgtgaatc cgtctgtttg ctcagtgaca
    13981 gaataattta ctgggtcgat ttctttattt ccatcatata aggcataagc atacgttcct
    14041 gaagaatcat tatcaaatgt taaagcagcg tcatgagtat cgataagttt gaatgttgtg
    14101 tacttgtttt gcgttccttc tttatcagcg atacctaatg ggatattgac agaaatctca
    14161 tattgaattt tttcaccgat tgcgacatct tttccattca attgaggtgt tgttttatca
    14221 actttagacg tatcattttt gatggtcttt tctactggtg tttggctatt tgccaggatc
    14281 tcaaaaggcg tgattgtttg cttttcaatc atttccgcat tatcaggagc ttttacttct
    14341 tctaaattat attttccaac ttctaaatga ttaacgatca actggccttt ttcaatagat
    14401 gcctcggcaa agtcattgtt gccgatgtca taagaatgac cagtaatgaa atgtttggct
    14461 ttggtttgat cagttgtcca agtgtacaat ccatctgtga cactttggat gtattttttg
    14521 acgcttggtg ttccttcttc tttagaaata ataaattctg ctccatttag tgcttcgttt
    14581 tcggcagtac cgatttttgt aactttcaac gttccatcat taccgactgt atttttaggg
    14641 tagagatgga tagtatctag ttcttctgtc ccgtatttat aagagccatc tgcttgtttg
    14701 atcatctcat atacagggaa agctaaaacc atgtttgcgg cagctgacac tccgtctttt
    14761 ggttcttctt tgatcgtgta gactgcatct ttcccatttt gttttttagg taaagataaa
    14821 gtgacatttc catcagcatc tgtcgttcct gaagcaactg gtgtaccagg agtcaaagac
    14881 tggactgctt gttttgctgc atccacggac gctcctttat ctcgttgcgc ataaaattct
    14941 tgagtgacgt tataaactga aaatgaaata tcggctaatc cttggtattg atcgaattcg
    15001 ctcatttctt tcccgctgtt ttggattaaa ggatcgggta aatcagtcat tttcttttta
    15061 tgaaggatga cttgagctgc tgtctcctct gcaaacactt tttttgcgcc gaagctgttt
    15121 gtgagtaatg gtaaaataag gaaaaggact cctaacataa cgtttatttt tttatgattt
    15181 ttcatttttt cactcctgtt ctttttttga taaacaacca gattgcaaag cttgcgaata
    15241 ccataccaac aacgcctaaa agtgtatttt tcgtttcgtt tgtttttgga agaaatggac
    15301 ggctattttc ttttttattg actactttca atatactgtc cgcctcttta tcctcagaaa
    15361 gaaaagttga aatggtaaac ggaatttctg tttcacttaa aatataacca gaaggtgctt
    15421 cgatctcttt taatcgataa cttcctgttt tcaacccgga aattgaaaaa gcgccatttt
    15481 tatcggaaat caggtgaagc gcatctgatt cgttctttgt ccacttgtat ccgttggctg
    15541 tttgctggag gtaatttcct gctttgtttt taataaggaa tactgcgcca gctaatcgtt
    15601 tatctggttt atttgtctcg attttgacaa attgcttact ccctgtatat acagagactt
    15661 tctttgtact gacaccaaaa ccaaaatcca gtgttccgtt gttgttgatt tccttgttcg
    15721 cctgtgctgt actgcttagc tgcatctgat aagaaatggt cagttttttt ccagcatatt
    15781 tttccaacga agggatcgaa aataaaacat caaatccgtg aggtttcttg tgtatgacaa
    15841 agccttctgt cagcttttct ccattcgatg aaatcgttaa actattaggt aaaaacgtta
    15901 atgcttcatc cgcactatct gacaattcga acttttgata gtcaaggata tttacaggaa
    15961 ttgtcgtatg caaggaataa gtgatttttt caccaatcgt aaaatcattt ctaggctctt
    16021 ctaatacctt ctcaaaaggt ggatcagggt agtcgttttc ttcattttta ggatacaaat
    16081 ggatagttga aagtttttga ttgttttgtc catgtacagg aagaacaact accatattct
    16141 ctgccttttc tttgacgact tctggtactt tggattcaat gaaaagatac gctttatctc
    16201 ttttttcttg atctttagcg gccagagaaa aagaagcgat accatcttcg ttgttaagag
    16261 ttgtagttgt tgcttctgcg gtaaacatac cggaagacgc accgtttttt gcgatctctg
    16321 cttgtgcttc ttctaccgtt ttgccctttt cccgtagatg gtaaaaagaa tctgtgacat
    16381 cataaacttg gaatgtgaca ccatttaatc ctcgatacgt ttgtaataaa gctttttctt
    16441 gtccgtcatt tggatgattt ttcggcagtt gcccgtttgg gaaaagcaat ttgtgtaaag
    16501 taatctgaac catttctgtt tctgtttctg ttgctacctg agtaaaagct ggtttaaata
    16561 gtaacaagaa gagacacata ctaagccaac caagtttttt catatttagc tgccccctct
    16621 ttcttttttc aaaaaaagat accaaataga aaacgcacaa ccaatcatcc ctactagata
    16681 gattcctaaa cggccaattc ctcccgtttc aggtaatggt acttttgcct gattcgtgat
    16741 gtctaaagaa atctggttgt ttttggctcc tggtgacaga acagattcat gatcttgtcc
    16801 atccacttga attgacccat cttcgtgtat aactatagta actggctctt ttagaccttg
    16861 gtatccttct ggtgtaaaag tttcggtcag cgtataagtt ccaggtttta aattttcgaa
    16921 tagaaaggta tctgtttctt gtccatcttt tggcgattct aagtccattt ctggtccctg
    16981 cagtctgaat tttgctcctt ttaatgtctg tccgttgtct tcttttttgt gtaccgttag
    17041 atcaaacgct tttagtcgat tttgatgagt gagcgtccat acattatttg tcatctcggt
    17101 attttttccc gtgtaaatga tttttccata tttatcgatt tggaattcat aatttcccgg
    17161 cgaagtatca tatccggcag gaccagctgt ttcaacgact cgatatttac caggtgtgag
    17221 tgaatcaaaa ctggcaagac ctttttcatt cgttgtttgg ctgtcaattg gctgataagt
    17281 accatttgct tcttttttct gtagggaaaa tgctgctcct ttaagattta tctctttgtc
    17341 cgtcccttgc atcgcatact tacgtactgc tacaggaact tcaacaaaag gattttcaat
    17401 agtcaactgg atcgtatcat cggaagtagt gactgttttt ccatcaatgg ttaccgtacc
    17461 atcagaagcg atcttgattt cccaagtagt ctttttgctt aatccatgcc cttctggagc
    17521 ttttgtttct gtcagcgtat aggtcatttc tttttgcaat ttgacatttt ctggaagaga
    17581 ataggttccg tcgccatgat ctgttagtaa agtatcaata gaatctcctg ttaatttgaa
    17641 aacagcgcca ataagatcct tttcacctgt agaggaattt ttcgttattt tcaagtttaa
    17701 cggtgtgaat tgtttagtat ttttccatgt cattgcatct atttgttgag aatcgtatcc
    17761 tggtacaggt aattctttga ttgtttggta actgaatgct tgaccttgat tattgtattg
    17821 aggtagtgat aatatctctt gataactttc tccgctattt gcagataatt tgtcaatgtc
    17881 tgcacgttcc cacgtatttg ttgtatcttt agctggttta atgattcgaa tatatccgtt
    17941 tttccaagct gcagcatttg tcgtatgttc ccgttgaatc tcaaaagtaa cttgatctgg
    18001 acgatcagct agattgttgt caaattcttc ccataacttt ttgatgtgaa gactgactcc
    18061 gggagcttta gcagatggta taccgaattc agctaattca ttggtatcaa tgtttggctg
    18121 gaaagttgtc ctgccgttca tttgatacca gaaatttgga tggaagtcct cattttctgt
    18181 ttggattctc acttggtaat ggatttggat ttcttggtct tttcctaaat agatctgatt
    18241 gctcttgatg gtatttcctt ctatggaaat agatggagtg accgttgtag gacttgtccc
    18301 cacactcttg actgaaagtg taccaggctg ataaatgaat ggttctgcga ttggatcatt
    18361 tatttgtcca ttgcttacag ttgctgagat ctgtacagct tttttggcta ggtattcaga
    18421 gatatctgtt gcatgatcag ctgattcata gtaaagatcg cctttttcat ctgatgaaac
    18481 catttgtcgc atacgagact ctacttctgc ttttgagaga ccagctgccg gatcgctttg
    18541 aagttggatg ccaagaccat gtatttcgat tcctcgttct ttgagtgcca tcgcttctcc
    18601 gatggttgcg ataaacgtac tgtcgattct tctggataga ttgttttggt caggtgcatc
    18661 atagattctt gagattagag aagtatttcc cggccgatct tgcgtattag aaaactgagt
    18721 tccgtaataa ttgctgcttg attgtgcgtg tacccgctgt actttatagg aaaatgttgg
    18781 tacaccatcc gtcagcaaaa cgatcacttt tttatgtcca tttggaacgg atagcatgct
    18841 tcctgcatct cttagtgctt tttgtgtaaa agtaccacca tttgtccgtg aaggtgtaat
    18901 ggattttact tgatttttca ctgaatcaaa tgaacccatc tgtactgcac cgttactgta
    18961 gctatatcct tcgcttgagt agccgacata tcccatattg attttgtctg tgataccgct
    19021 atctgctaaa gtatcgacaa aacgatcgac accaatcttt acttcaccga tccgattatt
    19081 gtcgttcata cttcctgacc agtctacgac caatacgaga tcaagaggcg tgatatcttt
    19141 ttgtacattt ccacgagcat tcaaataaac atcaaacaat ccgggtgtac ttgtttcctt
    19201 tgcatacttt cgtatcgcat agtctgcttg attaccggtt cctccatatt cgatatacga
    19261 atgggtccga tcattaggat ctccattcca agaatttata ccatcccatt gatttgttcc
    19321 gtttttattg ccttgatgat caagaacatt tgtatttcct gtaggttgcc aactggcttt
    19381 aggataagtg ccctgatcat ctgttgtata ttctgtagga atcgtttgat tagatggcac
    19441 actcacagct ggcaaggata atgtaggcaa tgtaaatggg ctgacagaat tttcgttttc
    19501 ttttgatgat tcttcttcta tttcgtctgg ctttggtact gacacatctg tatctttctt
    19561 attttctgtc tcatcaataa tgtcatattc tttattatcg acgatcagct tgtttgcctg
    19621 gctgtggtaa agtgctggat cgattggaaa actcagatcg atagtttgtt tagtgtttga
    19681 tgagatttcc aacgtaattt tcccgctatt tacctgataa gcatagccat ctccttcttt
    19741 tctttccaca tcacttaagt aattttcgac tagaccaatt tctttttcta tcttttcagc
    19801 actggtgtta ttcgcattta gcttgatgtt cagtgctttt tttccagact gatccgaaat
    19861 gacttcagct gaaattgttt ctaaaactgt tggatcatct gtagtgtctg ctattgcaga
    19921 taatggcaca aagtttgata aactcaatat tatcagcatg aaaataactt tcagtttctt
    19981 ccctgtggtc gtcatttttc ccctcctttt tctttttttc tgttttgacg tgaatagtga
    20041 aaattattaa aataaacagg cgttacataa ttttatttat tttaatatga taattattat
    20101 acgaagggcg ctaaagggaa tcacaccaat tttcatacca attggtttca tttatgaagt
    20161 tgattccttc gattaatgag cgtttcattg aaagaaaagg gttgagggag aaaaacatat
    20221 gatcaggttg ttggaaggaa actatagaaa gatggaaaaa atcattcgaa ttctgtccat
    20281 ttctgacaag tggatgacta aaaaagaact tgccaaacga attggtagtt cagaatctac
    20341 atttgtaagg tatatcgaag agatcaaaat ccgctgggga acggatgtaa cgattcaatc
    20401 ttcacaaaaa ttggggtatc gtcttgaaca tttgaatgta ccagcttatt taaatgttct
    20461 gatggaaatg tcgcaaagtt ctacaaacag tcgtttatta aatgagatta ttgtcaatcc
    20521 gggtaaaacg attgagtatt attgtgaaaa aatttccatc agccggtctt cttttacacg
    20581 taagttgaaa caatgcaatc aaattttaga aagctattta ttaaaagtga tcgttgacca
    20641 aggttttcag ttaactagcc ttaaaagtga gctccagttg cgtatctttg ttaccttttt
    20701 ctttttaagt tactacggaa gacgcacgtt gccatttcca atcgaaaaaa agaagatcaa
    20761 agaaattatg gtcaagaata attgtcagat gaatttgttt caggaagaaa gcccatatga
    20821 gcaatctttc tttattatgt attttgttgt gcatctcatg agagaatccc aaggaactcc
    20881 atttgaatgg aaagctggag actcttatgt acagttgata aatatcgata aaaaagactt
    20941 cttaatcttg aaaaatctct taggaaaatt gaagtacagc agctatataa aagtagtgaa
    21001 ctattttgtc aatacgattt tttcagaatt ttcggagaaa gaaaaaaatg aaatcagtga
    21061 gcagatacga aaaaatatgt cctactgttc gttttcatcc gctgattgca aagaaaaaaa
    21121 cgaagaatat gttgttcaat tattaacgca aatttatttt ttctcccgta ttttcccata
    21181 cgatacgact catttgacga tccgtgcaac ttactttgca cagcagatgc aacaccgcca
    21241 gatacgtcta tatgctggat ttcgtgcaga acttcaattt tattccactc tactgaatca
    21301 gaatctgatt tattatttat ctagctatat cttttggatg cttatcggta atcctgaaat
    21361 tcaccattat acttctgata aaaaaatact gattatcagc gacttaagtc ttcgacatag
    21421 tcaatatatt gaagaatata tcagtgatat tcttgctgtg cataaaattc actctgagac
    21481 aactgctatt actgaagatc agctgtcgaa atacaatctt ctcgagtatg atctgatcgt
    21541 tacgaatcaa cccattttga atgcccatgt tcctcatata ttgattgatg atagtgtgtc
    21601 attcgcaaat gaagaagaac tcatccgctt attcgagtta tgaataatca aaaaacagct
    21661 atgtataaac caccctgata aaaatttcag gacaaataca catagctgtt tctttttatt
    21721 gtattccttc agcaatccaa cgagatttat agcgaaagtc ttttaacaac tcttcttttg
    21781 tccaattatt tgcaatgaga tccgtcaaag tgttcattac gagggtcatg gaaaagtata
    21841 ggaaactaac tgcttcttcc gacgattgaa tggagaaatg attgattctt aataattcca
    21901 gccatttttc gttcattgga gaatcagccg atagttctct acgtttggtt gtctgctgat
    21961 catttcgctg ggttaacaag tataaccttt gccaatagat actttcgtga tctaagtcgc
    22021 agcaccactc taaatatttt tcgatcccca aaaagaaatg ttgtttgttt tcgctaatga
    22081 attttaagat atcttgatgg actatgtgag aactgctttt gatcgtatac aaataagcat
    22141 cttccaaatc ttgaaaatat ttataaaaag caccgcgcga catccccatc ttgtcaacga
    22201 tttctgagac tttgacttgg ctgatgtgct gtgagcaaaa tatctccagt aataggtcat
    22261 cgattctctg tttctttgcg ttcgataaat tgaaaaaagt ttcttttggc atccacatct
    22321 ctcctttatc tctgattata cgtggatagc ccttcattgt catgaaggat tcattccttc
    22381 tttttctctt cttctgtcaa gtacccatct tccattcgat aaacgcgatc actccattga
    22441 atcatacgga catcatgagt aaccataata gtcgcttttt gtttctcatg agcttcttta
    22501 actagtaatt tgacgacttc atatgcatga tctgtatcca aactagctgt tggttcgtct
    22561 gctaagatca agcttggctc attatataaa gcgcgagcaa ttgccactct ctgtctttct
    22621 ccaccagaaa ggtctttagg ataactattt ttaagtgact ggatatctaa agattgcagc
    22681 aaggaatcga tttttgttga atcttctttt tttgaagtaa cttggtcaac tagttcaaat
    22741 tgttccttta ccgttaaaaa aggaatcaaa ttagagcttt ggagaataaa tcctatttct
    22801 ttaaagcgta aatctgctcg ttttttttct ggaagctcag ttagtgagtg accgttgatc
    22861 actatttctc cggaagtcgg tgtttgcaat cctcctgaaa tggtaaggaa agtactttta
    22921 cctgaaccag aaggaccaat gatgctgacg aattctcctt cctttacata aaaatcaatt
    22981 gctttcaaag ctgagacctt tgtatgacca gaaccaaatg ttttggtaac ttttttcatc
    23041 gttaatattt ttttcatttt tattaacctc ctattgctgt aattgggtct actttggtta
    23101 tcgtacgtat cgaaaacaat ccgcctagca ttgcgacaat gatcaaaatc ccactatata
    23161 gcaaccattg tgaccaaaaa acagcaaaag gcatcgcgct tggtaaaatc agactagtca
    23221 aataagcaaa caaaagagca agcagaacgc ctaccacccc aacaataaag gactgtgcta
    23281 ctaaagattt agcgatgaag aaatttttga tcccttgtgc tttcattact ccaaaaatag
    23341 cagtcttctg aagtgtcatc acgtacatga aaataccgac cacggcagca accactaaga
    23401 acaaaaagta tatcatcgca tttaaggtca tattttgggc agaatagcca ggaatacttt
    23461 caatgaatgt cccaatagtt aataactgta gacccttcga tgctttttct ttcgaaattt
    23521 tagctgaatc ctttattacg atcccattga tcggctgttc ttcttcagaa gcaaacggct
    23581 gttgtccata cttcaaagca gtccacgtat ctagggacgc atagatgacg ggagaaactg
    23641 tgtaataagt ttcaggaaag ataccgacaa tcgtcaaagg atcgtcgtaa ctgccaattt
    23701 tgatcgtatc tccaatttta tagcctgaat cagccagatt ttgagagatg atgatttcat
    23761 tctttgtttt aaattcatgc cctttcgtga gttttggtaa taaaaatgct tctttcgttg
    23821 tgccgaaaac tgtaacattt tctttgtttt ttcctttgat tgctccgctg tacaatccga
    23881 ttggtgcttt ctctcctcct tcaatgtatt tcagatctcc tcttgttaat tgtgaagctg
    23941 caaatacttg atttgcatcc tccgacaaaa caatctcttg tgcgtcccaa tcatcgattg
    24001 cttttttaaa ctcttctgaa agtccattcg ctagtcctga caacatgaat acgacatacg
    24061 caatcaagaa cataataccg acgatcaagc catatcgtaa tttagaatag cgaatttctt
    24121 tgatagctag aaacatttgt ttttcctccc tatgatagat tgatagtgac aaaatgtcac
    24181 ttgttgaata gtgtaactct aaagtgacaa tttgtcactt gtgttgtttt tttcttatca
    24241 ataaattgtc taagacatct ttcgataaaa ccttaacatg agtcataaaa aatttattta
    24301 agccttattt atgctttcca tgataaacta aagtgaccaa atcaatgagg tgggaaaatg
    24361 attaaactat tagtagtaga agacgataaa gtattatcca ataatataaa agaaatactc
    24421 gctgcaatcg gcgagataac acaagtttat gatggtagtg aaggactgtt cgagttggaa
    24481 agtggagttt atgatcttgc aatcttggat ttgatgatgc cggaaatgaa tggatatgaa
    24541 gtgttagcaa aaatacggaa agaaaaaata cagactccag tattgatttt gactgcaaaa
    24601 gatagtttgg aagataagat tgaaggattt gaacgaggtg cagatgatta tctaacaaaa
    24661 cctttctatc gagaagaact gatcatgcgc gtgaaagcat tattgaaacg ctcgctaggt
    24721 ctctttgatg aaaatgttct gaagtataag gatatcagtt gtgatttaaa aagcaatgaa
    24781 gtcttgtata aagaacaagt tttgcccata caaggaaaag agtttgaatt gctcgtttat
    24841 tttttacaaa ataagggaat cattttgaca aaagagcaga tatttgaccg gatctgggga
    24901 tttgattctg atacgacgat tacagttatt gaagtttata tgagcaactt acgaaaacat
    24961 ctgcgaagta ctggattgga taaagaaatc aaaaccttac gtaatgtcgg ttatatactg
    25021 caaagggtgt gatgcaatga atggagatat tacacgaaag caacgtagtc gcttttttgc
    25081 gacaaatgta gcagcatttg cttttatctt ttttcttttg ggattcatta ctttacagat
    25141 attaaaccaa tctgcataca gagaaacaga tctgtcatta gagaaaataa attttgattc
    25201 tcgaacagta caattagaga tacaaagata tcagcaaggc gatccctttt taaacccgac
    25261 tcaaacggaa caaaacagaa caaacatgga acagccatta atggaaccat caaatgaccg
    25321 ttttaacact caagtgatct tgtggtctaa aaatggtgag atattgaata aagcagctat
    25381 tggtgggcgt ttcaaccaaa tcaaagaatt aaaaatggat acgaaaaatc tgaataccat
    25441 ccaaaatatt gaattaaatg aagacagtgg ctcatctgcc ctctctttcc gttcgattac
    25501 gaaaaaagcg gagacgaatc aagatggcgt tgcctacatc caaattttag aaaatacaaa
    25561 tcaaatcaag aattcattag cgacttttcg cacgattatg attttgtgta tgattgtttt
    25621 ttggattctc tctattggta tcagttatta cttgtctagt ctaagcatgc gccctattat
    25681 tctttcttgg agaagacaaa aagaatttgt tgagaatgct tctcatgaat tgcgcacacc
    25741 gttaacgatt attcaaaata gtttggaaca tctttttacc aagcctaatc atactattat
    25801 tgaagaatcc gaaagcatcg ctcaagcatt gtcagagacg aggcgcctca ctggattgac
    25861 ttcggatttg ctgacaatcg ctcggaatga ttctgatcag cagctgcttt caaagcaaat
    25921 gatcaataca caagaatata tcgaacaact tgttaaacca tttcaagaga tggccatcat
    25981 agatggaaaa gagtttattc tccataacga tgcaacatta gaagtaccaa ttgatgaaaa
    26041 aaaaatccat caagttttgg ttattttgct ggataatgca ttaaaatata cgaatccaga
    26101 agataagatc attttggatt catctgttaa ccataaagaa tggctgattc acgtaaagaa
    26161 tactggccct tctatcagtg aagaagatca agcaaggatt tttgatcgat tttatcggga
    26221 agatcaatct cgggcgaaag agactggcgg atacgggctt ggcttggcaa ttgcaaaaca
    26281 ggtaatcgaa cagcatcatg gacagatttc tgtatccaat tatcttccac aaggagtcga
    26341 ttttaaagtc caattgccgt tgaagcctaa aaaataatgc ttatccctaa aaaagggctg
    26401 tgacaaaaat cttgtcacag cccttttttg ttgcgaataa acggtgttcc agaagtagct
    26461 tcttcggaaa taagccgaaa tttctttaag ctgaccactt ctgtcacaac ctctttagct
    26521 tacgcttacg attttttatt gcggtcttca atgtgtacat caccgcttgt gtcaatatcc
    26581 aattcttctc ggctaacatc ttctgtcact ttttcaacat cttcatgttc ttctttatga
    26641 atcccaactt cttcacgaat aactgtatgt ttgcttacat ctacttgctc ttctttgatt
    26701 gggatagtaa tcgtgtcatc ttccaatttt tcgtctgttc cctgtgaaga ttgatctgtt
    26761 actggtttgc gttcgatgac gatttcttca cgtttgaccg gtacttctac cgtttcagta
    26821 tcgttgacca catgtttgtg gatgtcgact tctccagttg tcacatcatg tgtatttaca
    26881 tctaattttt cttcttttag ttgaattgta tcatcatcta gatttggttt gcgatcgatg
    26941 tcttcagtct tgcttgtacc cattgtatca gtctcgccag gtacgtccat cggatcagtt
    27001 cctggtgtca tattcaatgg atccgttgta ttttgaaagc ctgtcgtgcc acctacgtcc
    27061 attggatctg ttccagtgcc agtttctaat ccagtcccca ttgtgcttgc tgggctaaca
    27121 gtatcttgtg tatccattgt atcttctgtt gtttctggct gacgatagcc ttttactgtg
    27181 atgacaaatt ttccatcaga aatatctcct ctatattgat ataaaggatc attttctggt
    27241 gtagccgtct cagaatcata ggtatcaaaa gaaaaagtat cttttatttt ttcccataaa
    27301 gaacggtctt cttcaccatc catccgattt tcatctgttt caacatctat accagacaat
    27361 ccgtcaaatc ctagtctaga tgctgcttca tcagtagtat aaataacgat atcatctcgt
    27421 tcataccctt cgttacgcaa ccgttcaatt actgataatg cttcttccct cgtactatag
    27481 cttccgatga ccgttaaatt tttctcttcc atgtaaattc ctcctagcaa atagatttat
    27541 gattggtata ttgttactta taaatttatt ctataggaga cgattgtttt ctacaaatat
    27601 ttagattgga atttgattaa aacaatttaa ttgaaatttg aaaacattta tgttaatgaa
    27661 agtcatattc taataaaatg tcatttgttt ctatatgaat ttttatgaaa aatttcatat
    27721 aatttccact cgttgtgatg gtttaattta tccaatccat caataagcaa acaacttata
    27781 taaaaaagga ttaattctca atataattgc tcctaccata gaataaatct tcatgtaaag
    27841 taatgaagta ccaactattt ctaatacttc tttaagaatt atcccaacaa tatcgttaga
    27901 caaaccatta ttgaataaat ggagagccaa tcgattctga aaaaatgttt tctaattttt
    27961 cggaaataaa agaaagagcc tcctgccatt agaacgaacc aaatccattt cattgctcat
    28021 gaagtaacca catagctaaa aagaggataa atcaattaaa agatcaaaag aatgactgca
    28081 ctaataaaat aactaaaaat gtatcttctt acttcatatc ttttagctag tacttagtta
    28141 aaaatttgct tcctaagatc aagacaaact gtctatcttc atttgatgaa atgcggtaaa
    28201 atatagaaaa tgaacactat tggagggaat ttgtaatgga cagcaaaact agaaaacaac
    28261 tgcaagaaaa gttaaagcaa cgtacacact tgctcccttc cgtctttcaa cagtttttag
    28321 aagaaaatcg ttcttccctt tcattaggca gtcgctatga atacgcaaaa gattttcatc
    28381 tgtttactga ttatttgaga aaagtatttt ctgaagaagt ggatgatcag ctgattatcc
    28441 atttagaaaa acaagattat cagaatttct tagctaaaat ctatcgacat actcgttcgt
    28501 tccaaactac agcgaaccaa gaaacggagc aattgtttga aaatagctat tacgggattt
    28561 caaggaagca atattcatta aatcattttt tacgattttt gtatcaaaaa ggactgattg
    28621 aagaagagat ttctcttttg ggtacaagtt taccggaaac aaccaaagca gctccgcgat
    28681 atatcgatgc agaacttttc aaagattttg agcatttgtt tatcccgatt gaaggctttc
    28741 aaacaagcag agaagctagc tttgaagaaa agaatagacc gaggaacctt gcaattactg
    28801 ccattcttct gtattgcgga ttaaagatcc atgaagtagt agaacttaaa cgtaaagatg
    28861 tatctttgac caagcagatt cttaatctga atagaaaaga aaacagactg aataaagtac
    28921 caataccaaa agaagcgatg ccatttctgg agaactattt taagttaaca gcacaaaata
    28981 cggacgaaaa ttcttttgtt tttcgttcct cacatgatca gcaaatttct cctcggacta
    29041 ttcgccaaat tttgagcaaa attacagtat ataaaaacgt atcgccagaa ctatgcagaa
    29101 aaacatatcg ttattatgca gaactatatt tagatgatgc tgacgcggta aattatttat
    29161 gtggtaatcg atacctctca tcccattctt ttcaagatat ctgggaaaag atggttgatt
    29221 tttcttatca cgaattagcg gcattcgttc gggtttcttc catataaaac aaatgtatga
    29281 tctttttttc acaataaact atgttattat ataaaggatt atgttacgat tatcttgaat
    29341 ttgtatatta cttaacaaaa gataatggag tgtgaatgat ggagaccata acgtattccc
    29401 ctttgaactt atttacgaat tttcaagaag ctgcagctaa cttttccgag accccgatta
    29461 tctttgatga gccactttca gctttcccag aattaggact tgaaacaact tacaaaaaat
    29521 gctgcgaaag tgtgttgaaa cgggcttatc aattggctca tcttggagtg aaatccggag
    29581 acaaagttat tatttataag agttccgctt ttgacacata ccttctagct gtttctgtgt
    29641 cttaccttgg tgcagtgcct gtcatgactt cttatcatct accaacaaca acaatggaag
    29701 tatttatcga tcgtttagaa gaccctttca ttttatttga tgatgagaca aaaaagcgag
    29761 tgagagaaat ttcaaatgga acaaaatcaa aacagattcc tattctttat ttaatggaac
    29821 aacctgcttt gtctgtttct caatcatttt tagacaaaaa tgaaatttct tatatgaccc
    29881 atacttctgg tacaactggt atccctaaac ttatttgtca ttctgctcat tctatgggat
    29941 ggcggacgaa atggcaaaaa acagttttca caaaaatttc ggaaaagaaa ttaatcgctt
    30001 ttcacatctc tccagtgcat tccagattca atattggtat ctcttctttg atgtccatgg
    30061 gatttcctat gatgccttta gcaaacgcac aaagttcaaa agttgttcat atgttggaag
    30121 ctcaccggcc aattgcacta gagacccatc cgaataactt tgttcagtgg cgttttactg
    30181 ctaaggagca tccagaagct ttttcgggta tccgatacta tcactccact tttgatgcta
    30241 taaacaacca gacaatgaag acatttttgc gaacgtcgat ggcacaggat gcggtatttc
    30301 ttcaagtata tggccaaagt gaatgcggac caatgattct taaggctcat acactagaga
    30361 gtttgaaaac aagtgatgca agagatatgg gcgttggact ggaagacatg acaagcgctc
    30421 ggattacaga ttcagttggg aatgtcctac ctgaaaacac agatggtcat atacaattac
    30481 tgtctaaagg acgggctttg acttattaca aggaagatgc acggttccag gaaaatgttt
    30541 atggcgattg gtgggatagc ggagattacg gttttatgga taagcatggc cacctatttt
    30601 tgaaagatcg gcaagtggat ttgatcgaaa cgatcaatag tacattagct attgaagact
    30661 ttttattaga ttcgttagat tttttggcag aagtagtgat tgtaagggat aaaaatcatt
    30721 ctccacaacc gattattgct ttagctcctg ataaagaaat ggattggaat cgatggtggg
    30781 agcaagtcca tgaacttcct cgcttgaacg tccctatcat ccgtgatttt gatgcaatcc
    30841 cgcgaacagc tacaatgaaa gtgcaacggc ttcaaatcga gaaagaatta aaatcacaaa
    30901 agtcctaact atttaaggaa agaaagatcc attttggtct ttcttttctt tttgtttttt
    30961 taaattggat gagataaaaa tatgccaatt ggctgttttt cttttttgaa aatttcgtta
    31021 tactcttatt catggaggaa aatgttatga aaagaatctt gacaattggc ggttctgatc
    31081 catttgctgg tggaggaatc caaacagatt taaaaacttt tgagaatttt ggtctattcg
    31141 gactaagcgc cttgacttct ataggggcac tagatattaa tgagcgattt atgctggaat
    31201 ctatatcttt ggaattgctt aaacgacagt tagtttcgat tgataaaatg acttcattag
    31261 atggtatcaa aattgggtta ttgaattcaa gtgaagcaat catggttgtt cgagacttca
    31321 taaaaacaaa acaaaatatc ccaatcattt tagaccctgt tttggcattc aaagaaactg
    31381 aagctgtgat caatcaattg tatatttcgc agctcatcga agaacttttt ccacttgtta
    31441 cgatgattac gcctaatttg gcagaagctg ctttactttc tggtcaagct cttccctctt
    31501 cgttaacaga aatgatccat ctcgctaaag aactcactgc ttcaggaaca aaaaacattg
    31561 tgatcaaagg cggcacaagg atggctggag atgaagctgt ggatcttctc tattcaattg
    31621 atcattatga ggtattttct cataaaaaaa tcgacactac aacagtaaac ggggccggtt
    31681 gtgccttttc ttctgcaata acagcgaatc ttgtgttagg ggcagatttt accaaagcag
    31741 tagcagaaag taaaaaattt gttgcccatt gtatttctaa tggcgtgctg atgaacgatc
    31801 ataccggaag tgtctggtct ggcggtattc aaaaaggagg aatgagaaat gaaaactaag
    31861 caattaacga tctatgctat gctgattgcg ttgacagttg ccctttcatt aacaattttg
    31921 atccctgtac cggctacgaa tggatttgtg acattatgcg aggccgggat ctataccgct
    31981 gcttttcttt atggtccaac gggtggatta gttgttggtg cttccagtgg tctattgatc
    32041 gatttgattt caggatatcc gcagtgggca gttttttcat tagtgatcca cgggttacaa
    32101 ggatatgttg ctggttcttt cagcaaatct tctactcgta tgtggggatt agggctttta
    32161 ttaggaagct tagttatgat catcggctat ggtatcgccg gttggatttt atatgattgg
    32221 ccggctggag tggcttctat accaggaaac atcttccaaa atctgattgg aatgggactt
    32281 gcctttccta taactgcaag tcttcaacgt attcaagcac ggaagcaata ttaattgaaa
    32341 aagaaggttt tagaaaatga agttgtcaga aaaagaatta aaagaccaat tgactgaaat
    32401 agttaatgac atattggctg aggcacactt gaaaaaaggt gatctttttg ttttaggatg
    32461 cagcacaagc gaagtcgttg gaggacatat tggaaaaaat tccagtgcgg aagttggtca
    32521 atggatcatc cgtacgttaa aagagttgtt ggatcctaaa gaaattgcgc tagctgtcca
    32581 aggttgtgaa catttgaacc gagcattagt agtagaacga acagtggcag aagcaaaaaa
    32641 tttcgaaatt gtctctgttg ttcctgcact acatgctgga ggtgcttgtt ctgttgctgc
    32701 ttttgaccag ttcaatgatc ctgtcgaagt ggaacacgta gttggacaag ccggtatcga
    32761 tatcggtgac acatcaatcg gtatgcatat caagcatgtc caagtacctg tcagacctcg
    32821 tttgaaaacc cttggacaag ctcacgtgac agcccttcgc tctcgtccta aatatatcgg
    32881 tggtcctaga gcgaattatt gaaatgagta attgacagag caatctgaaa aacttcctcg
    32941 caaagtccaa ctaatgaact ttaacgagga agttttactt gtttgtttta tataaccgag
    33001 cttcataagg tgctagtatg cctttagctt caaattctcc gctagaggaa tttgacaaga
    33061 gtaatgtcca ggactgatct gcttcttttg gaagttcata gtcagcaagt gattctgtta
    33121 agtttaccat aataagatat tgttcatctt ctaaatatct ttcatagaca taaagttgtg
    33181 gatgttcatg aagatattct ttataagaac cgtatatcag acctttattg gtttgtctaa
    33241 tgcggatcat gttcttatag aattgcagga ccgactctgg ttcatattct tgttgttcta
    33301 cattgatcgt tttggtattt ggattagtga tcagccaagg ttcatgtgta gaaaaaccag
    33361 catattgttc cgaagtccat tgcatgggtg tcctgctgtt gtctctcgtt gtttcacgaa
    33421 tgatatccaa tgcttcttct cttgtcttac cttccgcaag cagcctttta taaagtcttt
    33481 tcgaatctac tgcatcaact tgatcgatcg attcaaaggg catattcgtc attccgattt
    33541 cctgtccttg ataaataaat ggcgtacctt gcaaaaagaa atacatcata gcgattgctt
    33601 ttgcagacat agcccaaaaa tcgggacgag tatctccaaa cactgaaacg gcacgaggaa
    33661 catcatggtt ttccatatat aaagcgttcc atccgatacc atccaatgaa gtttgccaag
    33721 cgcttaatgc atgtttcaat ttaaggacat ctaattgccc ttctttttct tgctgccaga
    33781 tatggatatg atcaaattcg aatatcatat cgaaataacc gtctttccct acccattgtg
    33841 gtccttcttc tgcagtgaca ccacttgctt ctccgacagt catgatatcg tactcttcaa
    33901 aaacatgttt caaatcggtc aaatgttctt cgattcctga gacattttga aagggtgaga
    33961 aagggttctc tgtggctttt actgaatatt catctttttt tacatgagaa atcgcatcta
    34021 aacgaaatcc atctatccct ttatccaacc accatcggat catattgaat agttcttctt
    34081 tcaatttctc gctttcccaa tttaagtctg gttgttcttt tgcaaaaaca tgcagataat
    34141 actgtccagt ttcatgagaa tactcccaag cagaaccgcc gaaaatcgat gtccaattat
    34201 tcggttcttt gccatctacc ccgtcgaccc aaatgtaata atcgcgatac gggctttctt
    34261 tagatttttt cgattcttca aaccattgat gctgatccga actatggttg atgacaagat
    34321 ccataatcac tttgatccct aattcatgtg cttcttttag caataaatca aaatcttcca
    34381 tcgttccgaa tctctcttct atatcttgat agtcacttac gtcataacca ttatcgacaa
    34441 atggagattg atagatcgga ttgatccaga taaagccgat accaagatct ttcaaataat
    34501 taagtttttc acggatccca ttgatgtctc cttgaccatc gccattgcta tctttgaaac
    34561 tagcaggata gatttgatag cctactgctt ctttccacca tttttgttct tgtatcatat
    34621 cgtttcactc ctttaacggt ccctcttgat tataaccgat tctattttag aagtatcctt
    34681 gttaccggta actagaaacg aaaagctcaa cgaatctgct aagtgcgttc gctgagcttt
    34741 attttatcat agatagcggg caatcagttg aatcatttgc ttgggaaatt cattcaagtc
    34801 agtaatatct acaaaacgac tttcaccgta gatcgacttg atttcttctt tgtcttggcc
    34861 aatagctgcg gagacaaata ggattccttg acgttcatat tctttcaaaa cagactggat
    34921 atcttctttt gcttttttgc ctgtataatc cggcaacgct tttggctgac cgtcactaat
    34981 attaagcaac agtttggttg ttgcggattg tctgtttaat ttttctgctg ctaaacgaag
    35041 tgcagcacca tcacgattgt tttgccttgg tttcatagtg actaattttt catttaacca
    35101 ttgaaatccg tcttcaaatt ctttataaga aaacaaagaa gtcttttctc ttgacgaacg
    35161 gtctgctgta tctccgtaaa tcaacaatgg gatatcgact ttcttagaaa attcggcaat
    35221 ggcaatcgct gctttttttg ccgcttcgat ccgatcatcc cgtatcattg acccagattc
    35281 atcgatccgt acagctaccg ctaaagaagg ttgctcatgt ggcgggttct ttttgtcaaa
    35341 atttctcaaa tcaccatatg ctactttgct tgcattgaac cgctgacctt cgtattttcc
    35401 tttttggtac gtctctgttt gttcattatc cagtaactct aaaatttgcc ggctaagtga
    35461 ttctactatt ggcattactt gcatgtgtag ctcacgtgct tccggcgttt ttccttggaa
    35521 agctggtcgg tgaacgatga gaccttcttt ttcatgaatc gtttccttca tgatttgtcg
    35581 cgcttcttta ttcagttgct tcttgatttc cttatcatgt gcttccatgt cagaagcaga
    35641 catttcttga gaatcatctc ctgtttgttg ttctttttga ttttttcctt cgatatcgct
    35701 actgcgttct ttttgcattt gaggaggaat ctcgtttttt tctattgttt cttcttgttt
    35761 ctcttctccc tcatttggtt cttcagcaga caacgatttc ttttcatcta agcgcagaat
    35821 ttcttcagca ttttcagtga tgtttcgttc atttgcttct tcggcgctaa tggttctttc
    35881 ttttgtatgc tcagattgag gatgacccca tttttctttt ctcaatatgt cctgaagttc
    35941 cagtagcaat ccactgtcag acaaagcttg ttcaagaatt gccaattcat ctgaatcagt
    36001 ggataattta tacaaaactt ttggataaat cgactggagt aaatcttgtc cttgcttata
    36061 ggtatttgcc caataaaaat aagaacgcat tcctgctaca cctttgatgg cgttagcttt
    36121 tgcagtttca tccaacaaac ggataatctc tgccatcttt aagagaagtt cttgtttttg
    36181 gatcatagtt ttcgccatcg cacgttctgc catgacttct aaggcaggaa gatccatttt
    36241 ttccgcatgt tgcatccgat ctcttaaaga ttcatttaat gggcgatttc cttggtaatt
    36301 tcgatttgta gtgatcacaa tgacgcaatc tggatggcga cgaatgattc ctgtcggtaa
    36361 attcaacatc ccatttggtt cgagcgctga gttaagtgcc acaagaacag aggcatctcg
    36421 gatgacagtc ggctcttgaa tctctaataa atagcctttt tcaatcgctc gaacgatttc
    36481 agaagggtaa tagctgtaat ggattgcatc tggataatca gcttcgatcc tttgaactag
    36541 ctctgctaat ttttctttag cagaaaaagg atcaatgccg taatggttct ggatcagtgt
    36601 cagtacagct tcaagtgtat ctgtttggta aatagcttct agtaattctt ggtcttgttc
    36661 cttattagaa tctactacag gtaataaggc tccgaaaaca tctgatttgt ccatatctgc
    36721 aaaacaggtc acttttgtat agggaagttg cagatcagca gatagtgctt tggctaattg
    36781 tgtcttcccc gacccggcat ctccttccaa aagtacgtta ctgattttca tttcaggatc
    36841 ttgccaattc cctttgattt cggaggcgat ccgccattct tctgcacttg tccgatggct
    36901 tttcggtttc ttccagatca tttgcttttc tagccgtgaa aaggttcggt tttctgttag
    36961 tgagaaattc tccatgcttc tcttcccctt ttgatcatct ctgtctatta atatcctagc
    37021 tcatctaaaa gacgttttaa aatacggaat cgttccccaa tcagttctcc gtctttttgt
    37081 agtgtatcgt aatacaaaat gatgtcttca tcgatctttg gatttttggt atctacttct
    37141 accgtcattc ggttcccttg taatccaatg atgtctacga ctggattttc tggatcataa
    37201 aatttttcat aacgataagc atcttggaag tataaacttc cttgcgcagc taagatcgct
    37261 aaatctaaaa tacgtgtgat tggtaattct tccgactgtc gtgaccattt ttctccagta
    37321 tgtcgccata ccttgcctga gatatcgact tttccacgat cattccactg agcaaggccc
    37381 agtgaaagcc cttttgcatc catatttaat gcatttcttc catcaacttt atcatagtct
    37441 tctacaacga ttactggttt atgttttaaa tttgttggta tttccattat tttaactcct
    37501 catcctttta tttttagtaa ataagtaaat tagtagttta ctcatttact aaactatatg
    37561 cgacattata gaggattgaa gtgaggattg caaagaaaaa gagcttcatt gatagaaata
    37621 aaaacaccat ctcgttcttt tacacgctca ttagtaacat gtagaaagtc caagatgatg
    37681 ttggaatatt t
//
LOCUS       DAJSAW010000026        37245 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJSAW010000026 DAJSAW010000000
VERSION     DAJSAW010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.026"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..839
                     /locus_tag="ONT64_001426"
     CDS             <1..839
                     /locus_tag="ONT64_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2302850.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
     gene            complement(1263..1433)
                     /locus_tag="ONT64_001427"
     CDS             complement(1263..1433)
                     /locus_tag="ONT64_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2302851.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1627..1806)
                     /locus_tag="ONT64_001428"
     CDS             complement(1627..1806)
                     /locus_tag="ONT64_001428"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302852.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2193..3305
                     /locus_tag="ONT64_001429"
     CDS             2193..3305
                     /locus_tag="ONT64_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR2302853.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3383..3676)
                     /locus_tag="ONT64_001430"
     CDS             complement(3383..3676)
                     /locus_tag="ONT64_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR2302854.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3844..4158
                     /locus_tag="ONT64_001431"
     CDS             3844..4158
                     /locus_tag="ONT64_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HCR2302855.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4179..6158
                     /locus_tag="ONT64_001432"
     CDS             4179..6158
                     /locus_tag="ONT64_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR2302856.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6171..6497
                     /locus_tag="ONT64_001433"
     CDS             6171..6497
                     /locus_tag="ONT64_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HCR2302857.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6513..7031
                     /locus_tag="ONT64_001434"
     CDS             6513..7031
                     /locus_tag="ONT64_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302858.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7031..8383
                     /gene="celB"
                     /locus_tag="ONT64_001435"
     CDS             7031..8383
                     /gene="celB"
                     /locus_tag="ONT64_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCR2302859.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8467..9972)
                     /locus_tag="ONT64_001436"
     CDS             complement(8467..9972)
                     /locus_tag="ONT64_001436"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCR2302860.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10064..10654)
                     /locus_tag="ONT64_001437"
     CDS             complement(10064..10654)
                     /locus_tag="ONT64_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2302861.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10833..12479
                     /locus_tag="ONT64_001438"
     CDS             10833..12479
                     /locus_tag="ONT64_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR2302862.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12528..13247
                     /locus_tag="ONT64_001439"
     CDS             12528..13247
                     /locus_tag="ONT64_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCR2302863.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13726..14673
                     /locus_tag="ONT64_001440"
     CDS             13726..14673
                     /locus_tag="ONT64_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2302864.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14685..15605
                     /locus_tag="ONT64_001441"
     CDS             14685..15605
                     /locus_tag="ONT64_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2302865.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15646..17103
                     /locus_tag="ONT64_001442"
     CDS             15646..17103
                     /locus_tag="ONT64_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2302866.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17448..18062
                     /locus_tag="ONT64_001443"
     CDS             17448..18062
                     /locus_tag="ONT64_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR2302867.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18055..19779
                     /locus_tag="ONT64_001444"
     CDS             18055..19779
                     /locus_tag="ONT64_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2302868.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19776..21227
                     /locus_tag="ONT64_001445"
     CDS             19776..21227
                     /locus_tag="ONT64_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2302869.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21700..23853)
                     /locus_tag="ONT64_001446"
     CDS             complement(21700..23853)
                     /locus_tag="ONT64_001446"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HCR2302870.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23855..24901)
                     /locus_tag="ONT64_001447"
     CDS             complement(23855..24901)
                     /locus_tag="ONT64_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2302871.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25051..26340
                     /locus_tag="ONT64_001448"
     CDS             25051..26340
                     /locus_tag="ONT64_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HCR2302872.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26353..29055
                     /locus_tag="ONT64_001449"
     CDS             26353..29055
                     /locus_tag="ONT64_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR2302873.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29040..29897
                     /locus_tag="ONT64_001450"
     CDS             29040..29897
                     /locus_tag="ONT64_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2302874.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29968..30867)
                     /locus_tag="ONT64_001451"
     CDS             complement(29968..30867)
                     /locus_tag="ONT64_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR2302875.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            31036..33207
                     /locus_tag="ONT64_001452"
     CDS             31036..33207
                     /locus_tag="ONT64_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCR2302876.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33275..33616
                     /locus_tag="ONT64_001453"
     CDS             33275..33616
                     /locus_tag="ONT64_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR2302877.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33892..35646)
                     /locus_tag="ONT64_001454"
     CDS             complement(33892..35646)
                     /locus_tag="ONT64_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2302878.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35657..36493)
                     /locus_tag="ONT64_001455"
     CDS             complement(35657..36493)
                     /locus_tag="ONT64_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR2302879.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36495..37178)
                     /locus_tag="ONT64_001456"
     CDS             complement(36495..37178)
                     /locus_tag="ONT64_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HCR2302880.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12016 a         6552 c         7639 g        11038 t
ORIGIN      
        1 catatcagct ggtagaaact caagcgccaa cggggtatga attagatgcg acaccgatcg
       61 agtttgagat cgaaagaagt cagacagcgg tggtagaatt gacgaaagaa aaccgactta
      121 caccaggcgg tgtcgtgttg acaaagatcg atgaccaaag cggggaagtc ctgcaaggag
      181 ctgtttttga gttacaagat gccaatggaa ccgttctgca gtcaggtcta accacaggag
      241 cagatggaaa actggcgatt gacggcttag caccaggagt gtatcagctg gtagaaaccc
      301 aagcgccaac ggggtatgaa ttggatgcga caccaatcga gtttgagatc gaaagaagtc
      361 agacagcggt ggtagaattg acgaaagaaa atcgacttac accaggcggt gtcgtgttga
      421 caaagatcga tgaccaaagc ggagaagtcc tgcaaggagc tgtttttgag ttacaagatg
      481 ccaatggaac cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgatag
      541 atggcttagc accaggagca tatcagctgg tagaaactca agcgccaacg gggtatgaat
      601 tggatgcgac accagtaact ttcaagatcg aaaaagaaca agaagtggcg atctttttaa
      661 caaaagagaa tagaaaagtt gctgatagct cagatataag aggaaacaat ccaacaagca
      721 gtggaaataa gcatcttcca aaaactggag aaaccatcat tagccagttt atattgtcaa
      781 tattgggtgt gctacttgtt ttcatcagta taaaatttag aaagaaacga aatatatgag
      841 ctaaaataaa aaattgaata agcatagttt ttaaaatcaa ctagtaccca tcaattgaat
      901 ggaaactacc gattttagaa ggggtataca aacaaatatt agtatcggag tagagaaggt
      961 tgaacccaac cagtaaataa gtaaagacaa ttttgtgctg cgttgagaag tgaaaaataa
     1021 acctttgtag taatactagt taataaaaaa tatacaaaaa cactgcgaaa atcattcttg
     1081 agtttcgcag tgttttttgc ctaatgactc aagtttattg ggtaatactt tgattttctt
     1141 tctacaaata atcagcgatc agttgtaatt ttttctctag ttttaatggc tctttgtcaa
     1201 taaggagaaa aacaagtcgg gtcttttgtc tcggaaagct tcaaccaact cgtgaataaa
     1261 ggttacgcct ccttgaaagg cgtggaaaaa ccaagtaatc gatcaatcac caatttcgca
     1321 tttggtagaa catgtttggt gagctgaaag taggtggcgt tcatgtctgt aactaagaat
     1381 tctatttctt taggattagt gcagctaagg aaatagtttg ttaatcgagg aattttttgt
     1441 gtaggtaaat tttctcgatt aatttttctg tctcgccatc cgcggaaata aaactcagtt
     1501 tatcctcaac ggaaacgtga gaacgaaatt cacaaccctc aacactcaag acagaattct
     1561 tttggaagac tttggtaaat agcttctcaa ctctttcaat gtgcgaataa cggttgtcaa
     1621 agatacctaa caacttttcg aaataaaaaa taacgacact ttttcagtca gtaaagaagt
     1681 gattttatgt ctgacatgat ttgaaatcga gtgccttgga cggataaaat aactttgagc
     1741 gttccaatgg cttcgacagt ttttacaggt atagcgctgc ttttttaggc gtataaccat
     1801 tggcatatga ttatattatt caaagtggac aatggtttcc ttcttcttgt ttttcaccac
     1861 cattaatttc ctatttccat ctaccacagc agaaccacaa tttctacaga cacatggagc
     1921 agggaagaga actgcagcaa cgatcaatgt ttttttcttc tgtaaggttt cgtaagagac
     1981 ctctgtaatc atcaaatttt tgtctattga tctcagaatt tttttgatag aaccattcat
     2041 aaagcatatc gttctctcag ttgtttattt tgtggtgatt taatcataca agaaaacgat
     2101 atgttttttt ataaaaacat gaataggaaa aatagttgac aagaaaatga caacgccata
     2161 tgatgttcca tagtatttac aacctgtcat agatgactgt aaaaaataaa agtaaaaaga
     2221 aaggacgaca acaggtggat ttttatcagt ctctacaact agatccgttt attttaaaac
     2281 aaaaaatacg agaagcagct tctaaaaaag aaaagtgcat gtatatttgt tcattgtttt
     2341 tacgatcatt attcatcgta ttgtttgcca tttgttttat cattataatt acaacattat
     2401 ttgaatcgaa gcataaacca tacgctgtgg ttttattctg tatgttgatg agtattcgtt
     2461 ttgttgactt tggttataag atttcccatt cgatcatagg tttagcaatc gtcatgttct
     2521 cactattagt agcaccgtat gtccagctga tcaaatggtc agttatgggc atgttgattc
     2581 attttatttt actaagcagc atattaatgg caaccgctag tgatcccaaa atggggaatg
     2641 cttctttgta cggcttttca tatctcttta ttgtttattc gttacctaaa gattcgttga
     2701 acaagaattt ctttacacaa acaagtagct tattattttt gtttttttgt tggttttcag
     2761 ttctccttta cagaaaacat cgagaaaaga acaaagacaa aagcttgttt aaagagattt
     2821 ttttgaaagg catgtattcg caggaaaaaa tttggatgct catctatgca tttggcatta
     2881 gtcttttgat cgttgcagga gagtatgtcc cttttcagcg attgatgtgg gcagggtttg
     2941 cattttcttc tattgtatct agctatggat tgatgtcgat tggtttcaaa gaacgagcag
     3001 tagaccgaat cattggctca ttgattggat gtgttttatt tattggtatc agccaattta
     3061 ttccttttaa ctgggtcggt atattaggtg gtctagcttt aggtgtttgt tctacctata
     3121 gatataaaac agtttttaat tcttttggtg ctttggcaat cactgccagt ttattcgggg
     3181 ttccaggagc agtaacgatc cgcatttttg agaatatcct tggggtatgt ttaggaatca
     3241 tgtatattgg ggtgactgaa atacttatcc gaaaaataag agaaaaacac ggtttgaatc
     3301 attagatgag atcgaataaa aaataaggaa aaagtgaaga tacacatgga tgataaattg
     3361 ctatcattcc atgtgtatct ttttacggta aatcagtgaa acgttcgact ttcattttta
     3421 atttatactt tttacggagt tcgtttattt gagccatcat ttcggatttg tgatgctttt
     3481 cgattgcttc ttcatctttc caacggtcga tcagtaaaag agattctgga tcttctagtg
     3541 gaaagtaata gtcatatttc tcgtttcctt cttctgcacg aatcgctttg acaatgtttc
     3601 tttcgaccat ttcttctgca aattttctgg cttgtccatt tgtacttgta tacaaaatat
     3661 tcactgtgat actcactaaa tcccctcctg aaaaaattct atcactagta tatcacggtg
     3721 ccagaaatta ttattcaaga tatgatacac attttttccg caatgctacg gaaatatata
     3781 ttttgaatag aaaacgctta caagatatgc ttggtttata aaaataaaag gagtgattga
     3841 tttatgaaaa aagcattgat tatttgtgcg gcaggaatgt catcctcgat gatggcctca
     3901 aagacaacgg atttctttaa gcaaaaaaga gaagaggtac tcgtagatgc agtttcagct
     3961 acagaaggcg ataatatgat taagaatagt gattttgatt tatttctgat cagcccgcaa
     4021 acgacgatgt ttttggataa attcaaaaaa ttaggagaat ctgttggaaa gcctgttgtt
     4081 agtatccctt tccaagcgta tgtcccaatc cctacaggca tccagaagtt agcagaggtg
     4141 atcgaagaga acatatgatg gaataggagt ggatgataat gaaacagaag gaaaaattgc
     4201 tgttgagata tttgattgat cacaagaagg agtatgtcac cagtcaacgt ttagcttctg
     4261 aattgttatt atctgatcgg acaatccgta attacttgca gcggatcaaa gagttagtag
     4321 aaaaaaacgg aggtaagatc attgctaaac caggttatgg ctaccaactg catattttac
     4381 aaagactgac atttgacttg tttttgagtc ggcaagaaat agtacctgga tatgctagag
     4441 aaccgcaagg attttatgag tccgaagatc gacaaaagta tatattgaac caattgcttt
     4501 tggaagatgc cattcttttt atggatgatt tggcagaaga gctttatatc agtcgttcca
     4561 gtttgacaaa ggacatgcaa gaaatcaagg agcgattgat tccatattca ctaaaaatcg
     4621 tttctaagca tggtcaaggg acatggatcg aaggggaaga aagaaatcga cggcatttta
     4681 tcatggatac tttttttgga aaccattatg gaaattcatt gaaagaatat ttagggcaaa
     4741 gccgtttttt cccagacatc agttttgaag agctagtcat tatcattctc gatgaaacaa
     4801 gagaagctaa gttaaaagtt tcggatttta tcatacagaa tctgacttta cacttggctt
     4861 tggggatcaa acggctccga gaagggtttg aaatcaagga attaggcatc gaacaagaag
     4921 tttctaagcg agtagagtat caagtggcgc agcggatcgt tcaacgtatt gaggtaatcg
     4981 caggtgtatc gtttcccgaa gaagaaattg cttatttgac attacatctc atggctaagt
     5041 cgaatcacaa agaaaatcaa aatgatcagg aactatcaaa ggaattagcg aaagttctga
     5101 aaagattgtc tcaggaattt catttttcat tagtagagga tctccagctt ttcaatggac
     5161 tactcgatca tttgtcaccg atgatgattc gattgagccg aggtattcgc attgagaacc
     5221 cattgactga ggagataaag aaagaaaatc ccaaagtctt tgacgcagta aaaaggcatt
     5281 tcagcaacat gccggctttg aaaaactata ccatcaatga agatgaatgg gcctatttgg
     5341 ctttgcatct catggcggca ttggaaaaag aaagagctgc gcataaacta catgcattga
     5401 ttatctgtgc aacgggttac ggcagtgccc aactattaaa gaatcgcgta gtcagtgaat
     5461 tcggtaaaaa tattacagtg gtaagtgtaa aaggctatta tgagatcaat gaaacaactt
     5521 taaaaggaat cgacctgatt atttcttcta ttgatctttc gacaatgttt ttcaaaattc
     5581 cagttttaca tgtcagtatt tttttgaatg aagatgatgt tcgtaaaata cgaaaggtag
     5641 ttggagaatc tataccctat cattcctcag atagaccgat accagcattt tctctacagc
     5701 aaaaaaagca gatttacacc gaacagttgt ctgaaaaatt tttcaagact tatacgtacg
     5761 aaccgaaaaa ggaagaaatt cttcatgatt tgctgcaaaa gctgagcata aatgaagaag
     5821 aaggatatat aagagaaatg cgaaagcaga tccagcaacg tgaaaatatg ggacaaatca
     5881 tattcagtga tacagtggca gtcccacatc ccgctgttcc agttggaatc gaaacaaagg
     5941 tagccgttgc attgattcct tctgggatgc agtgggatca atatcaagaa attcattttg
     6001 tttttttagt ttcgccttct tatgtcgaaa atgaaggaat tacggtcgtg actaaagcaa
     6061 tcgttaggtt agttgaccag ctaaaagtac agcaggaaat cttagctgaa cctatttttg
     6121 agaatttcag cagtaaattt atcaaactga tcgaataagg aggaaaacag atggctgaaa
     6181 aaataaacag tgaaacacta caagtgactt cttttgatat cattttccat agcggaaatg
     6241 cacggacact gatacatgaa gcatttcgct cgatgcgtaa agagatgttt gaagaagtag
     6301 aagagaagtt gcgacaagca aatgaagaga ttcttcatgc gcatcagtca caaactcaac
     6361 ttctgaaaga atatgccagc gggacaaaaa tagaaatgga aattatcatg gtccatgcac
     6421 aagatcatct gatgacgacg atgacattat tggaagttgc acaggaaatg aattatttgt
     6481 atcaaaaaaa taaatagaaa ggaaggatca ttgtgcagga tacagtaaaa aggaaaatcg
     6541 aattagaaaa aaagcaactt agtttgaaaa acatgtattt caatcgttat ctaatcgtac
     6601 gttacctgac tgcctttttc tttttcatga acatgcaatg gatgattctg cttctatcag
     6661 ctaaaagttt aggaagtagt ttaccgatga ttctgatttt agctattttg cctgcagtag
     6721 gagaacaagt aaagctttat cgaaaacacc aaacgaacgt tccatggaca aagtggtatt
     6781 ttttgttcca gagtgtcttt aatttattag tgattccagt gttgtttacc tcaggattta
     6841 cattcttgta tccttttatg gcaaataaca atggaggaca gttatttgtt ttcattcttg
     6901 ttgtaagcgg tatttttgcc agtgcattta tacagtatcg tttaaagaaa atttcactca
     6961 accaagacca acagtatatc cgtattaaac aatatgaaaa agcgttatat ttaggaaagg
     7021 agaataacta atgtttgatt ttttgcagaa atacctgatg ggacctatgg ggaaaattgc
     7081 gcaacttaaa atcgttcggg ctgtcatggc agcggggatg gcttctattc ctttcacgat
     7141 tgtaggctcg atgtttttgg ttttaaatat tttgccatta ccgttcccat ttttagaagg
     7201 attttttaat gcaacttttt tcaaagttag tgacttgtac atgatcgtca atacaatgac
     7261 gatggggatt ttatctattt actttgccat tgtgtttgct tatgaattaa cgtcaattga
     7321 acgagatgaa caaaaattaa atgttaatcc gctcacgggg gcattactat ctgtttttgc
     7381 attctttatg tgtatccctg aattgatcat gactgatggt aaaatcagct tgattgcttc
     7441 tatcaatgag acagaaactg tagtaaatgg tattaggatg ggctcttttg tcgaacgttt
     7501 aggaacttca ggaatcttta cagctattat tatgtcgatt attgcggtcg aattgtattg
     7561 tatgtgtgtc aagagaaatt gggtcataaa aatgccagat gttgttccgc caggagtttc
     7621 tcgttctttc actgctctga tcccaacatt tgtcattgcg tttgtggtga tgatcatcaa
     7681 tggattatta gttgcactag gaacggatat cttcaaaatg attgctatcc catttgggtt
     7741 tgttacgcag ttgacgaata catggcttgg tgtcatggtg atttatttct tgatccatgc
     7801 attgtggatc gtagggatcc atggagcgaa tatcatcact tctttcttga ccccgatcgt
     7861 attagctaat atggcagcta acgcacaagg cgcaaattat ccgttggcag gagaattcaa
     7921 caattcttat gtaaccgttg gtggatcagg tgcgacttta ggattgatca tctttattgc
     7981 attcctcgcg aaatctgatc agctgaaagt gttaggaaaa gcctcacttg taccaggatt
     8041 attcaatatc aatgaaccaa tcattttcgg gatgccaatc gtctacaatc cctatttagc
     8101 ggttccgttt ttcttagctc cgatggcttc tgctacactc gcttattttg ccattcggct
     8161 ggaaatcgtc aaaccgatgc ttgcacagat gccgtggcct tcaccagtcg ggatcggagc
     8221 atttgttggt agtggtggtg attggaaagc tgctgtgtta gcagtcattt gtgcaatcat
     8281 cgcattcatt atctggttcc catttatcaa attctatgat aataaattat taaatgaaga
     8341 aagagaaaag gcagcagaat tagctagtga aaagttaaaa tgatttctaa gaaaaaagga
     8401 actgtgacaa aaaatcacag tccctttttt tcttagaaat gggaaaccac tagtatataa
     8461 ataccattat tcttcaatca accgcttcac gattgcataa tccccttcat aaggttcatc
     8521 tacaccattg attttttgat agggatccac gccttttccc gcaataacga ctgaatcttg
     8581 cggagcgctt tctgctaaag cgcgtcgaat cgcttcagga cgatccattt cataatgaac
     8641 gatcaaatct ggattagtaa ttgcttcatt gatttcttcc gcgatttttt tcggatcttc
     8701 aaatgctggg tcatctgccg tcaaaaaggc gatgtcagct gtttctgata gcacttttcc
     8761 aaaatcatgt cgacgggaga ttgctttgtt tcctgggctg cctaatacaa caattacacg
     8821 gccatctgga tgttcatttt tggcaaattt cagcaatgtt ttcaagctta gataattatg
     8881 tgcatagtcc acatagacat gcgctccatt ggattggatc aattgatcca ttcggcctgg
     8941 tacacgagct tcttttaatc cgtcttgtgc atcttgactg gaggcacctg ttagtgctgc
     9001 ggcaatgatc gcactggctg cattttcttg gttgaatcct ccagccagca aaatatcata
     9061 agcagtatcg cagatagcta agcgatcttc atgagaagat aaagtaaaac cgtgagtacc
     9121 tttatctgaa cggatcactt gataattgct ttcttccgaa cgaccataag tgatcgttgg
     9181 aatctggaat agatcggctg tttccctaag taaatcaaaa tagtcacttt catgatttaa
     9241 aacaactgtt tttgaattca agatcaattg tcgcttgcaa tagaaatagt catcaaatgt
     9301 cggatgttcg atcggactga tatggtcagg cgagatattc aagaagattc cgacatctaa
     9361 agtcaagccg tatacgcgtt gtgttttgta agcttgtgaa gaaacttcca tcactagatg
     9421 agtcatacca ttttcaactg cttctgacat catacgatat aaatctaatg actcaggcgt
     9481 tgtcaaatgg gatttgaaat atgttttgcc atctaacgtt gtattcatag tagaaagcaa
     9541 tgctgttttt ttatttgttg tgtgatccaa gattgctttt gtaaagtacg cagcagttgt
     9601 tttacctttt gttccagtga atccgatcac tttcaattta ttttgcggat agtcgtaaaa
     9661 agccatagca agaagcgcca ttgctttacg gatatccgtt acgatgattc ctactgctga
     9721 attttcgtat ggttgttctg atacataata ttgaataccc tgttcgattg ctgaatccaa
     9781 atattcttct ttgaaattca ttcctttaca gaaaaacaaa gtagaagcat cagcttttct
     9841 tgaatcataa cttaactgaa gaaattcagc tttttctggt acatcaagat gccagcctct
     9901 tgttgaaaca aattctttta atagattttc ttttaatagt aaatgacgga tgtcttctaa
     9961 agagaagctc attgattgat ccctcgcttt attcaatttc ttggtttatt atagcacact
    10021 ctttttaata tatcgtttgt ttccgctatg acaaaatcca gctttattca aattttctcg
    10081 acacagggga caagcggatg agtattttca taaacaatga actgactgta atagatagga
    10141 gaacgaatag tgtctccaac aatccaaaaa cataataatt caaaaagacg ataagactat
    10201 tggtaagtaa aagtccttgc ggcattggaa gccgaaaaaa tttgtggaaa ataatcggcg
    10261 gtatcgttat accgccattt gaggccccga ctgtgtacaa tgtgtaaacc ccaagactga
    10321 acaaaagact tcctgaaatc agtgaaaaga aatgtgagga gatcatttcc acttttggaa
    10381 caatctctaa aacaacagga aaaagcagac ttcccgctac agtcttaaaa aatactgatt
    10441 ttcctaatag gacagcggct agaagcagca tcataagatt gatgctccaa acagtaactg
    10501 agcgttggat agaaaaggcc gtttcaacta aatgtccgat ggcgcccact ccggcggaag
    10561 ctatatcgtg agggagcaag aaccaattca atccaactgt gataaagaaa agacctaaaa
    10621 atataacggg tagttttttt atttcttgtg tcattgatat ctcctataaa tgttagccat
    10681 aaaaggctgt atatttcttt actttaccaa aaaaacgaaa ccgaattcaa aaaaacaatt
    10741 gttcttataa aagttatata aaatcgattg ccagtttctg ttttaccaat taaaatgata
    10801 gtaatgtttt gtagaaagtt ggaatctgac cgatggataa tcaaagaaaa ccagcattag
    10861 atcaattaac accgcaagaa aaaatggcac gaggctctgc ttggatgacc gccagtaata
    10921 tcatttcaag attgctaggc gctgtctata tcattccctg gtatgcttgg atgggcgaaa
    10981 atgctaaggc tgccaatgga ttattcaata tgggatacaa tatatatgca ttgtttctac
    11041 tgatctctac agcaggaata cctgctgcga ttgcaaaaca aactgcacgc tataattcat
    11101 tgaatgaata tggcacttca cgacgattat ttatccgtgc attacagatg atgggcgggt
    11161 taggcctgtt gtttgctttg ttcatgtaca tcgcctctcc ttggctagct catgcttctg
    11221 gtggcggaga agaactgatt ccaacgatgc gttctttgag tatcgctgta ttggtttttc
    11281 cttgtatgag tgtgatacga gggtatttgc aaggcaatca agaaatgatg ccttatgcgc
    11341 tgtcgcagat tgtagaacag gtggcacgta ttttttatat gctattggct acattcatca
    11401 tcatgaaagt catggatggt gactatgtga cggcggtcac gcaatcaact ttcgcggcat
    11461 ttatcggtat gctagcaagt attcttgtcc ttttgtattt tttgaagaaa catcaagcgt
    11521 atacatcggc gtttattcac tacagcgaaa acaaaataaa tattgcaacg aaggaattgt
    11581 tgttagatac cgttaaagaa gcgattccgt ttattattgt gggttcaggg gtaacgatct
    11641 tcaaattagt cgatcagttc acttttatga agatcatgtc tgcatccacc gagtattcca
    11701 atgcacagtt actggatctt ttttcgattt ttagtgccaa tccagataag ttgacgatgg
    11761 tagttattgc attggcaaca tcgatcgcgt ctactgggtt accgttgatt acagaagctg
    11821 ttacgttgaa agatcgagta ggtctggcaa aattgacaag cggaaattta cagttgtttt
    11881 cattttttat gtttccggcg acatttggtg ttatgttact tgcctatccg ctaaatacat
    11941 tattttatac accggattct ctaggaagta atgtactgat ccaagcaagt tttgtaggac
    12001 tcttcctagg actatatatg ctagtttcca acatgctgca aggaatgttt gaaaacaagg
    12061 cggcaattaa ttatttggtt attgggtttc tagtgaaact ccttcttcaa tacccagcca
    12121 ttcgtttatt tgaagtatac ggccctttat tggcgacaat gatcggattt gctgtgtctt
    12181 gtaccttgat cttaaagaga atccatgaag tagcccgctt taaaccaagt tttgtctggc
    12241 gcaggacatt gctgatcttt attcttacct tgattatgct tttggcttcg tggatcacaa
    12301 agatgatttt tggtgctttt ttaaacgaag atagcaaatt ccagtctcta ttgttgattc
    12361 ttgtagtagc aggtataggg gctttagtgt atggttattt agctttgaaa atccgcttgg
    12421 cagaaagact actaggacca ggaatagcgc gtttacgaca taaattaaaa ataaaataag
    12481 tgaatcagaa gagctagatt gatgctcttt tgaaatgggg gaaacgcatg cgtttagata
    12541 agtttttagc agatatggga cttggcagcc gcaaagaagt caaaggatta atcaaaaaag
    12601 gaacgatcga agtcaatgga caagtggtga aaagcgataa agcgcaagta aatgaattcg
    12661 aagatgcggt tacttattta ggagaaatca ttacgtatca aaaagatttt tactacattt
    12721 tgcataaacc tgcaggagtc atttctgcta ctcaggataa tcatgatcaa accgtaatcg
    12781 atttattgga agaccaggat tatcgggaag atctttttcc agtcggccga ttagataaag
    12841 acacagaggg cctcttgatt ttaacgaatg acggggtgtt tgcccatcag ttactatcac
    12901 ctaaaaaaca tgtggagaaa gaatatctag ctgaggttag cggtataatg acagaagaag
    12961 atatccgttt atttgcagaa ggactgatta ttgatggcga tgagcagaca ttgccggcaa
    13021 aactccaaat tgatcaagta gatcaagaga cagaaacttc aattatccgt ctgattttgc
    13081 atgaaggaaa gtttcaccaa gttaagcgaa tggtcaaagc agtaggaaaa gaagtcttat
    13141 atttaaaaag aatccgtatg ggaaaatttt ggctgcctaa agatttgaat aaagggcaat
    13201 atcgacccat gaccaaagaa gagctagaac aagtgagaga aaaataattg aatggatagc
    13261 taaaagaata agcagagtag ttgtagcgcc atctgaaaag cagacttgtc cgtctgactt
    13321 tcggatggct tttttgtgaa atttcggctt atttccgtag aagctacttc tggaacaccg
    13381 tttgttcgga ataagggact gtgacaagac ttttgttaca gtccccattg ttccttgtga
    13441 ctcagagtta gatagtaagg acacgatctc cctaatactc aggccaaaac gctgaattca
    13501 caacctctga taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa
    13561 aatatgagga actattttcc cgtttttttc tctaatactc aggccaaaac gctgatttca
    13621 caacctctcc tatactttgt ttcattttcc ctatactttg tttatttatt ctcattattg
    13681 aaagcgctat aattacgttg cgaatgaatc gaaaggggtt tttagatgaa aaaacgaaag
    13741 gggttcaatc atttttttat gaatctatgg cgttataaag cacttgtcct gatggctgta
    13801 ccgggtatgc tttggatgat ttttttcttt tacatacctg taatggcgaa tgtcgtagca
    13861 ttcaaaaatt ttcatatttc agcagatggg ttcttggcta gcttgaggga aagtccttgg
    13921 gtgggttggg aaaatttcaa atttttattt tcttcggatc aagcatttct catcactaaa
    13981 aatacaattt tatataatgt agcatttatc cttttgaatc tgctgatctc agtcgtattt
    14041 gcaatcatta tgagcgagtt gagaaataaa cgactcgtta aagtttatca aacgatgtcc
    14101 ttactacctt attttctatc ttgggtcatt atcagttact ttgtttacgc ttttttaagt
    14161 cctgacaaag gaatattcaa tcagtggatc actggacatg gaggagaagc tgtcaattgg
    14221 tataatgaac cgaaatattg gccgtttatc ttggtgttca ttggtacatg gaaagggatt
    14281 ggttataaca gcattattta ctttgcatct gttatgggaa tcgatccgac ttattatgaa
    14341 gctgctatgg ttgatggagc tagcaaatgg cagcagatca aacatgtgac gataccacaa
    14401 cttattccac taatgacgat tttaacgatt ttagccgtag gaaacatttt ccgagctgat
    14461 tttggcctgt tttataacat tcctaggaat tccggtgcat tatatgatgt gactcaagtt
    14521 ttggatacgt atatctacaa tggcctgaca tccacaggag attttggtat gacggctgct
    14581 gcaggacttt accaatcagt tgtcggcttt gtcttgttga tgatcacaaa tacgatcgcc
    14641 cgacgttttg atagcgattc tgcattattc taaggaggaa aaaaatgaaa aaaaagaaag
    14701 tggagagagt ccaagtccgc tctttcaata agacggctaa ttttttctct aatataatga
    14761 ttgcactctt tgctatttcc tgtattttgc catttatatt tgttatcgcc atctctttta
    14821 ccgatgagtc catattgtcg actcacgggt acagtttttg gccagaaaag ttcagtatat
    14881 ttggttatac gtacttgttc gaccagatgc aggataaaat tttccaagcg cttttcgtca
    14941 ctgtacttgt tactgtttta ggtacactga tcaatagcac ggctacttct ttatatgctt
    15001 atgcgatttc acggtcaaac tttccattcc gtcgtttttt taccgttttt tgtttgatta
    15061 cgatgttgtt ctcaccaggg atggtggcca attatcttgt tatgacaaat cttcttcagt
    15121 taaaagatac gatttgggca ttgatcttgc caatggctgt cagtcccttc aatattattg
    15181 tgatgcgcac attctttaaa cgttcggtct ctgattcgat tattgaatcg gcacggatcg
    15241 atggagctag cgaactgcga atatttgttc agatcgtttt accgttagct gttccaggta
    15301 ttgcgacaat cagtttattt gccgcattgg gttactggaa tgactggttc aatgctttac
    15361 tatacatcca agatgataag ctagttccac tacagtatct tttgatgaaa atccaaagta
    15421 atattcaata tctgacacaa aattcaggag caggaagcca attgacaggc ggattagcct
    15481 cagttccagg agaatctgcc agaatggcga tcgtagtcat ctcgacgttg cctattgcga
    15541 tcagctatcc atttttccag aagcattttg tcaaaggtct aacaatcggc ggagtaaaag
    15601 aataaagaaa caaaaatata aatcataaaa ttaggaggaa gaaaaatgaa gttatggaag
    15661 aaactagcct ttacaggtgt ttcagcgttg ttgttgggaa cattagcagc atgcggaggt
    15721 tctggttcta aagaccaggc agaagctagc aattcggaca ctttgcagat gtatcaaatc
    15781 ggagataaac cagataattt cgatcaattg atggaagtag caaacaaacg aatcgaaaaa
    15841 gagatcggtg taaaagtcaa tatcaattac attggctggg gcgattacga aaagaaaatg
    15901 aacgtcatca tttcttccgg ggaaaattat gatatcgcct ttgcgaacaa ctatgtttcc
    15961 aatgcccaaa aaggagcctt tgctgatttg actgaattag cgcctaagta cgctgaaaaa
    16021 gcttataatg atttagatga agcatatatt aaaggaaatc tggtcaatgg aaaattatat
    16081 gctttcccag tcaatggaaa tgtctttgct caacaagtac ttacttttaa caaagcttta
    16141 ctagataaat acgatctttc aattgatgga atccaatctt acgctgatgc agaaaaagtc
    16201 ttgaaggaat tccatgaaaa agaaccaaac acggcagctt ttgccattgg acaaggcttc
    16261 aaagtccaag ggaactttga ttatccttta gggaatacgt taccatttgc tattgatctg
    16321 gatggagatg attcgaaaat catcaaccaa tacgataatg aaacgtatat cggactgtta
    16381 cgtacaatgc atagctggta caaacaagga ctcattccaa gtgacgctgc aacgagcaat
    16441 aaggattatc cactagaagg aaatacttgg ctaatgcgtg gagaaacaca aggaccgtat
    16501 gattatggcg atacaatttt gacaaatgca gcaaaacaag aacttgtctc aaaagcaatt
    16561 actgttccac taaaatctac agctcaagca caaatggcaa attttgtcgt atcaaatact
    16621 tcaaaaaata aagaaaaatc agtagaactt ctagggctgc ttaacagtga tccagaatta
    16681 ttgaatgggc ttgtttgggg gatcgaggga gaagcatggg aaaaagtcga tggttcagat
    16741 gataaaatca aattgctaga tggttatcag ccaaatacac atatgtctgc ttggaataca
    16801 ggaaacaaca agattctata tacacaagag tctattacgg atgacatgat tgccaaacgt
    16861 gatcaatcga ttgctgatgc agaaacttca ccgattttag gattcagctt caacactgac
    16921 agtgtcaaaa cagaattaag taatatttct aatgtcatga atcaatattt agatggattg
    16981 aatacaggga ctgttgatcc agatgaaaca ttaccgaaat tgaaagacgc tttggacaag
    17041 gctggctatg ataaggtatt gaaagaaatg caaaaacaat atgatgagtt ccgacaagaa
    17101 taataatgaa taaagacaaa ggggttgtga caaaagacgt gtcacaacct cttgttccga
    17161 ataaacggtg ttcaaaagcc agtttctcgg tattaagcta aatatatgta aaaatgtgga
    17221 aagccatatt cacaaaattt ttcttactac tcgaaactaa gcggctattt cacaaactct
    17281 tggtaaacgg tgttcaatca gctaaccctc ggtattcgct cacaaaacga caaaatatga
    17341 ggaactattt ttccgtttgt ttctctaata atcaggtcaa ggtgctgatt gcccaacctt
    17401 tttttgtata atgagaaagt ataaacttaa tgtttggagg agaagatatg ttagggaagg
    17461 ttttggaaga gttatttatc cggatctggg tcattataaa actaaccttg tatttctgga
    17521 tatatacatt tgccggcggg atcatctttg gattaggtgc ggcatggaaa acagtcaatg
    17581 aactatttta tttgtacgga ttcgagtata aagaaatcac aataaaaaga ggctggaata
    17641 tatataaaag aaatttttta agaggcaatc tgctatttag tttattttta tctggtactg
    17701 ctttattgag ttacaattta tatttatctg ttcaaatcaa agggctcatt tttttagcta
    17761 ttgattttat tttacttttt gccttgctct gcctatttgc tacttatcaa tacagtatga
    17821 ttttagatag tgaatatacg atctctgttc ctaatctgct aaagctaagt tttatttcgg
    17881 ttttttccag cttttcgtct tttctaaaaa taattattgg tagcggtata attttaggcg
    17941 tgacttggca attcaaagga ttgatcctgt ttggtgtcat tggtcttttg acggtttgga
    18001 atggtacgat gacaacacat tggcgtgaag aattagataa acagttggaa agttatgaat
    18061 aagtttcgga ttctattttc aaaaggaacc ttactaaatc gtctgttgcg gaaatacgcg
    18121 gttatcatga tcggtgtaac gatgactgct accgttattt ttagtatcca tacttgggaa
    18181 caaaatcaaa aacaggcaga aaacatgaca agtgatgccg ttcaaagcac aagcagaatg
    18241 ctgaatgata aaacaacctt gagtaggatc atcaaaaacc agcttgtagg gaattccgaa
    18301 aagatcgaaa atgtgacgac ctacttaaca aaacccatag atcagtatct gatgtatgta
    18361 tacgaacagc aaaacagtac cgatgaatta gtttctttcc caaatcaaat caaagatctc
    18421 tacgcaaatt atgaagaact aagtgcgata tatatcgtgt tgaatcaact accggagtat
    18481 tatgaatctt ctagagaaaa caaaggtggg gaaataaaaa aggggacacc aaaattacta
    18541 gacgcctttt atatcaagtt gcctatcaca caaggaggag ctgagtcagg aagtattttt
    18601 attggttttc aaaagaaaga actagatatg attttagaaa atctaacgtc attcaatgga
    18661 ctctctttat acatgatttc tggaacgaca aatcgactat acacattcca tgataataaa
    18721 atagctaaag aaacgtttga aagacaagaa atgatcatca cagagagtct gaagaaaaat
    18781 tcacaattgc cgattgacga tctggaaaaa aacaattttg ttcaacacca agaactgtct
    18841 gatgattatc gtatattagc tgtactagat aaaaaaagtg taaaaatcga aacgggaaaa
    18901 aatcttgccc cgctgatcat tggtatcatt attttagaca atatactgct tatctttctt
    18961 tataaaacat tcaaacgata ttcacagcaa gtatctatcg tcatgcaggc catggatcaa
    19021 acagcagcag ggaatttaga gacgcggatc gacataacaa acacggaata tgaactgagg
    19081 gaactttcaa tcggtatcaa cgaaatgttg gatagcatca atcagtttgt agaagacatc
    19141 tacaaattag aaatcaaaca gcaagacgca catatgcggg cgcttcaagc acagatcaat
    19201 ccacactttt tatataatac attagaatat atccgaatgt atgcaatcag cgaaggaagt
    19261 gaagaattag cagatgttgt gtatgcgttt tccgctttgc ttcgaaacaa tacgaatcaa
    19321 gagaaaacga tcactttaaa agaagaactg gacttttgcg aaaaatatgt ctatttgtat
    19381 caaatgcgtt atcctaatcg agtagcctat cattttatga tcgatcctga tttggaaaag
    19441 atcgaagtac ctaaatttgt gatccagcca ttagttgaaa attattttaa acacggtatt
    19501 gattttactc gattcgataa tgcgctaagt gtcaaagtct tgcaggaagg caaacgggta
    19561 aggatcatca tcaaagataa tggaaaaggg atgactgaaa aacggctaaa acagattgaa
    19621 gaaaaactgt ctcatcctaa agtggaactt cacggttcta tcggtctgca aaacgtgaat
    19681 gaacgtttac gtgccagctt cggcagcagt tattatatgt cgcttgagaa taatgagaca
    19741 ggtggactga ccgtttctat tacattcaag gagggatgag cgtgtatcaa gtgttattgg
    19801 tagatgatga atacatgatt ttgaatgggt taaaaaagat aattgattgg caaagtttag
    19861 gctttcaaat agtagcaaca gcagagaatg ccaaagaagg actagctgta cttgaacagc
    19921 gacagattga tttagtagta acggatgtga caatgccaga aataaatgga ctggaattta
    19981 ttgaagcggc tcaaaaagaa cgtcataatt ttgaatttat gattttatca ggataccaag
    20041 aattcgatta tttgaaagga ggtatgcaat taggcgccgt gaattatttg atgaaacccg
    20101 tgaataagtt cgaattggtt gaaagcctca agaaaatcaa aacacggtta gatcagcaaa
    20161 atgaacaaaa aaatcaacaa gaaatctatc aagaactgtt attcagtcaa tggttgaatg
    20221 aagaattgga tgaaataagt gaagaagaaa taataggaca aataagtgag aagcagcata
    20281 ggatagtatt gattcaactg gcaagaactc atgatactct tatcaataga tggttgcaag
    20341 aacggcaaca aatcttttac tatcaacgaa attacggaga ccgtgtattg atcacgttgt
    20401 tgttggaggc agatgaagtt gatggcttct gttgttttgt tcaagaaaac tttcctgatc
    20461 aggaatggct gatcagtatc ggggaagaga cagattcttt ggacaaaatc cctgaaacat
    20521 tccaacaagc gaaagacaat ctacagttgc atcaatttta tggagataaa gacgagcatg
    20581 ttttttacgc agtacaagca gaaacaaaag atcaaaccat cgatttttca agtttcagac
    20641 gcgtgctgca aaataaacaa ttggaagtcg ctcaaaaaat gatcactgat ttttttgaac
    20701 agtttcagtt agcagccatg atgccggaag atatacgata ttccagtttt ttactgttta
    20761 tggagattca acgggaattg attgatctgg aagatgagga atatttacaa ggaatcgaaa
    20821 aaatccatca ggcaaaaacg gtgcaagaac ttcagcagct acttctgtca ttcatcaaaa
    20881 agcatcagag acagaaaaag tacagtgaca atgtggaaaa agtaatcgta atcctgcacc
    20941 agcattatca agaaccactt acactcaaag aagtgtcaga aaacctgcat ttaaatgtca
    21001 tgtatctagg acagctgttc aaaaaagaaa cgaaaaaaag tttttcagct tatttgaacc
    21061 atttacgtat ggaaaaagcg aaacaactgc ttttgcacag taatcaaaac atcaatgaga
    21121 tagcaagtga gattggctac aacaacacca cgtatttctc taaattattc aagaaaatcg
    21181 tcggcagatc ccctaaagag taccgagaaa acagtggaga atcataaaag aaaaagccaa
    21241 tgtaatgaaa taacttataa aaaattgtct agaaatctaa ttttgggctg ttcttattac
    21301 ttgaggcaag gcagcttttt catgacctct cgataaacgg agttcaaaag ccagtttctc
    21361 ggtattaagc caaatttatg caaaaatgtg gaaagccatt ttcacaaaat ttttcttaat
    21421 actcgaaact aaacagcttt ttcacgacct ctccactaaa cggtgttcaa tcagctgacc
    21481 ctcggtatta gctcaaaaaa tggcaaaata tgaggaacta ttttgccatt tgtctctcta
    21541 atactcaggt cagaacgctg atttcacaac ctctcaataa acggtgttca aaagcttgca
    21601 tctgcggtaa taagaccaac caaacgaaaa atatgaggat ctatttttgc ttggctgttc
    21661 ttattacttg atgcaggaca gctttttcac aacctcttgt taattatagt ttgataagga
    21721 aaaaggcaaa tcttttgctt gatatttctt agggtgaggg atgatcccta attggtaaga
    21781 gatcgtgccg cctttcagca aatcttgatg ggtaaaatat aatttcgtat attctctgtc
    21841 catccgtttg attttatgga taaattgttg ctgcggccga ttaggcgttg cttcaatcgt
    21901 caattccttt ccatttgaca aaagcaaaac agccttgtca attaatggca taccgataac
    21961 atattcaccg gttccaggcg tgacaggata aaatcccaga ctattgaaga tataccaagc
    22021 ggccatactt ccattgtcct catctccagg gtaacccatt ggagaatcgt cgaaggtctg
    22081 agtcatcaac tgtttaagca gtggctgtgc catttcaggt tgtcctatat aactgaacaa
    22141 gtaaggatag tgaaaacttg gctggtttga aatagctaat tgtccaaatt ctatcgcggc
    22201 catctcactc atttcgtgga tctcaaaacc atatcctcct gggtcaaacg ttgggggctg
    22261 attgcacagc tcaatgagtt tttctttata tctctcactg ccaccatatt ttccgatcag
    22321 tcctgaaaaa tcgtgataaa cagcaaagct gctttgccaa gcacttcctt ctgcataatc
    22381 ttgtccccaa cgattagctg aaaatggttc tctgaacttg ccatctttat cttttgaacg
    22441 cataaatcct gactcatagt caaagatatt ttgatagttc aatgattgct gctgatattt
    22501 cttcgtcatt tcctcttctt ctagtacctt ggcaacttgg ctaatacaaa aatcgctata
    22561 agcatagtct aatgtgtgat tgactgattc gtggtagtga ttaggaacat agccatatgt
    22621 caagtaatct tctgttcctt ggcgtccata atttggatgt ttgctttgaa ccgtagcacc
    22681 tttttccatt gccgttagaa attcaggcat caagtctcgt cggatatctt ttacagcagc
    22741 atctgcaatg accgcatcga tcaatgtccc aggcataagt ccacgttcat caggagacag
    22801 ccattttggc aaatagccac tttctctata gctgttcaaa aaaccttcca gcatttcttc
    22861 gtatttttct acagcaatca aagaataaag aggataaacc gttttatacg tatcccaaaa
    22921 cccattattt gtatagagaa caccaggttt tatagatcga ctagtcgtat catagtggat
    22981 tttttgccca ctaggatcaa tttcataaaa tgtttgtgga aataaaaaca cccgatataa
    23041 attatgataa aacgtccgca attgttgttg atcatggtgt gtgagctgta tacgatcaaa
    23101 ataggactgc cattgttctt gactgtgagc aaggtaagtc tctttgcttt ggttttgttc
    23161 tagttccagg ttataagctg cttgttccga gctgataaaa gatgtcccaa atcggatagt
    23221 ttgttttttt gtctgttcaa aatacaacgg gatcgagtcg ctatttttgt tggaaacagc
    23281 taactcatca attgttaaag gatattcaaa tctcaatata aaataaaaag aaaaatcagg
    23341 atcttctgaa ccagcaaaat gtgtgatttt tccttccact gtgtaaggat ctataacaaa
    23401 gagctgatgg cgtcccggaa aggataaaag taaaccgctg tcatttttgc tgtaatcaat
    23461 agataagatt ccgccataca tgctaggtat caatgtagaa cagatgccat aacgtaattg
    23521 acagatcgac agatgagtcg ggcaaaatat actttcttct gggcggtaag aactttgagc
    23581 atggaaaagg gtgttctcct gtagttttcc attgatcggc gtaaacagca tatgactgaa
    23641 atcacccatc caaggacttg gttgatgggt aagacggtag ccttgaaaaa cgcggtcatc
    23701 aggatgaaac caccagctgc cgttttgatc cgtagtttgc ggtgcaaaat aattcatccc
    23761 aaaaggaacg ccagtataag gctggcagtt cccattagag aaatttggct gattcgctgt
    23821 tgcatgtcta gtatctattt gaccgatatt cattttattt ctcctttgtt gattgtcttt
    23881 tgatgaatga aactggaaat ttactatcgg atggtcgatc ttttgtctct aaccaagtga
    23941 ccaaagcatc tcccgcaagc ttacctaact gttcaaaatc ttgtgcgatg gttgtcagtg
    24001 gcgggtcaac aagagctgct gcttggatat catcaaatcc gataattgaa aaatcttcag
    24061 ggatatgata accatgctgt ctcaagagat tcatggtctg gatagcgaca acatcgtttt
    24121 cacaaacaaa agcagttact tcatacttag tgatataaga aagcaattca tctaatgtcg
    24181 cgcttttgtc atccagtgaa gtgtggaaag tcaaaccagc tttgtccaat gcttcaaggt
    24241 aaccaaggta acgctgtcga acggattgtg gatgagtctg cgtaccaaac aaataagcga
    24301 tttttgaatg tccctgttcg atcaaataag aggcagcaag cgcaccgcct tggaaattat
    24361 cggaaagaac ggtaggaaaa ggtaactcat agattttttt atctaatatg acgacaggga
    24421 attctttcaa tgaaagctca aaaagtaagt caagatgttg ttctgtatca agagcataat
    24481 aaatcaatcc gtcaaattca ttgattagct cttttgctgt tttattttgt agaaagtcaa
    24541 gcgtcgtcat catgacatca atctgtttat cttgcatcac agggttcaat cccttgctaa
    24601 aatcaccaac agacaaatca gcgacatatg gaagaagaaa cagaatccgt cgtgtttttg
    24661 taggagaagc aagaatctgt tccggagaag cggatttaac gaaactgcct ttcccgcgaa
    24721 cgcgataaat aagtcctaat tgctctaatt cagtcaatgc acgtttggaa gtgattctgc
    24781 ttactttata ggtacgtgaa agttcttttt cagttggtag ttggctgtct acaggtaatt
    24841 caccagttaa aatagccgtt tgcaaatcat taaatattgt ctggtacaat ggttgagtca
    24901 tataaacacc tcttaatgat atatcatatt caaataatac cataatggat gaaaaagtaa
    24961 ataaaaagat attttttgaa agcgtagaca taattggctg tatgaagtat aatctttaaa
    25021 gatatatcaa atagaaagaa ggttttatta gtggtttata aagatatacc aaaatcggtg
    25081 cagaaattta tggaagatat cacacaaaaa tgtggggata tccatgcgga ctgggcaaag
    25141 aattttacag cagcatttgc caatactctg ttgacaacag tcaaaaagca aaatgacgga
    25201 accacctttt tattgacggg agacattcca gcgatgtggt taagagactc tactgcacaa
    25261 gttcgtcctt atttagtgat tgcaaaagaa gatgaggaat tagctgacat gatcagcggg
    25321 ttagtcaaaa agcaattttt ttatatcaat atcgatccat atgcgaatgc cttcaacgaa
    25381 gaagctaacg gtgctggcca ccaaacagat catacaaaaa tgaacgattg gatctgggaa
    25441 agaaaatatg aaattgattc tttatgctac ccaatacagt tggcctatct tctttacaaa
    25501 aatacaggaa aaactgatca attcaatcaa tcttttattg aaggtatcaa aaaaatcttg
    25561 gacgtgttca aaacagagca agatcatgaa cactctcctt atacatttgt acgagacacg
    25621 acaagaaaag aagatacgct gatcaatgac gggcgaggtg caaaaacagc ttttacagga
    25681 atgacatggt ctggtttccg tcctagcgat gatgcttgcc gttatggata tctggtacca
    25741 tccaatatgt ttgcggtggt cgtgttaggt tatgtggaag aaatttttca aacgatactc
    25801 gatgataaag aaattgtaca gcaaacacag aaattgaaga aagagatcca agaggggatt
    25861 cagaatcacg gacgaacggt taatcaaaaa gggcaaacga tttatgcata tgaagtagat
    25921 ggaatgggaa acgccacagt catggatgac agtaatgtgc ccaatctgat cgccgcacct
    25981 tacttgggat attgttctga acaagaggaa gattatctgc aaacaagaag aacattgttg
    26041 agtaaagaaa atccatattt ctatgaagga aagtacgcaa aaggaattgg atcctcccat
    26101 acgccagaaa aatacgtttg gccgattgca ttagctatgg aaggaatgac aacgtcagac
    26161 aaatctgaaa aagagcggat attgaattta cttgtagcaa ctgacgcagg aacacacctt
    26221 atgcacgaag gattcgatgt gaacaatcct gaaaattata cgagagaatg gttttcttgg
    26281 gcaaatatga tgttttgcga attagtcatg gattactttg atattagagt agaaaagtag
    26341 gaggtatcgt aaatgaaaaa gaaagtatat atcatttctc atagccactg ggatcgtgaa
    26401 tggtacatgg cttacgaaca gcatcacatg cgactgattg aattgataga cgatctacta
    26461 gagctatttg aagttgatcc gtccttcaac agttttcatt tggatgggca aacgattatt
    26521 ttagatgact atcttcaagt aaggccagaa aaaagacagg caatccaaca agcaatcaat
    26581 gaaggaaaat tgagaattgg accattttat atcttgcaag atgattttct tatcagctcg
    26641 gaatcgaacg tgagaaatat gctgattggc atggaagaaa gccgtaaatg gggaacccct
    26701 gtcatgttag gctattttcc cgatacattt ggaaatatgg gacagacgcc tcaattaatg
    26761 aaacaagctg gtatttcagc agcagcgttt ggccgtggtg taaaaccaat tggctttgat
    26821 aatcaagttt tggaagcaga aaactattct tcccaatact cagagatgtg gtggaaaggc
    26881 cctgatcaaa cagcaatctt tggtctgcta tttgccaatt ggtatagcaa cgggaatgag
    26941 attccagtag aaaaagaagc agctcttgcc ttttggaagc aaaaattagc ggatgcagaa
    27001 caatatgcct ctacgaacca cttattgatg atgaatggtg ttgatcatca accggtacaa
    27061 aaagatatat caaaagcaat ccatctcgca aatgaactgt ttccagatta cgagtttatt
    27121 cattctaatt ttactgacta tctggaagca gttcaaaaag atgtgccaga agacttaggc
    27181 tcagtagaag gagaattgac aagtcaagaa acggatggct ggtatacttt agccaataca
    27241 gcatctgctc gggtttactt aaaacaatgg aataccaagg tccagcgaca gctagaaaat
    27301 attacagaac cacttgcgac gatggcttat gaagttagtg gaaactatcc ccatgatcaa
    27361 ttagattatg cttggaagac attaatgcaa aatcatcctc acgacagtat ttgcggttgt
    27421 tcagtcgatt ctgtccatcg ggaaatgata cctcgttttg aaaaagcaga tgaagttgga
    27481 aagtatcttg cacaagattc cttagaacag ctgaccgcag caatcgatac gaccggattc
    27541 ccaaaagaca gctttccatt tgtcattgta aatactgcag gaatggataa aactggtgaa
    27601 gcagaaataa ccatcgaatt agaaagaaaa agattcgctg aaggaatacc tgaacaacta
    27661 tatcaagagt tagaaaattt gcctaaacgt aagtatcatg tagaaacaaa aagtggggcc
    27721 actataccag ctattttatc tgaagaaact gtccaatttg gttatgattt gccgaaagac
    27781 cgtttccgtg tgccatatat ggcaagaatg atcaaagtga ctttaccatt agaaaacatg
    27841 ccagcatttt cttgggaaac attcgcgctt gtagaagggg aagcgaaagc agaagaaaag
    27901 gaaacaatga ttcatcagag tggacgaatc atcgaaaacg gccctctaca tttgacgatt
    27961 gaaaaaaatg gtaccattac aatggaagat cgcaaaaaca aacgaaagtt gaataatctg
    28021 catatttttg aagatatcgg cgatatagga aatgagtata tctttaaaca acctttctgt
    28081 gatcagccaa tcctatccag taacaaagaa aactctgaag taaaagtatt agtagataca
    28141 ccggaaatag caaaaataag tattctacag gaaatggaaa tccctgtttc tgcagacgag
    28201 cgtttagaaa aagaacagca gatggttgtg gaatttcgtt atcgaaaagc agaacgatcc
    28261 aaagaaaaac gaatccttca aataaaaaca atcatgacaa tcagaaaaga tagtaaaaaa
    28321 atcgactttg aaacgactct cgacaatcag atgaaagatc atcgcttacg tgtcttgttt
    28381 cctacaaagt tacatgtgga acatcacgaa gcagatagta tttttgaagt ggtgaagcgc
    28441 ccaaaccatg tgtctaaaag ttgggaaaac cccacgaatc cgcaacatca gcaagcattt
    28501 gtcaatattc atgatgaaga gtacggtgta acagtcggaa actttggttt gaatgaatat
    28561 gaagtaacag aagacggaca aatcgcagtg acgctacttc gcagtgtggg agaactaggc
    28621 gattggggat attttccaac accagaagca cagtgtctag gtgagcatcg gttcaattac
    28681 agcattgaac tacatggacc agaagaaaaa ttctctactt atttgcatgc atacgcagca
    28741 cagattcctt tttccactca acaaatcaaa catcatgaag gaacattgat ttcaaaacaa
    28801 caatatttaa cgataaaaag tgaaacgttt gcaattacag ctttgaaacg cagcaagttt
    28861 agtgataaag tagtagtaag aggtttcaat atgtctagtc atttagaaaa attggaaata
    28921 actaaagaca atggaaaaac tgtcatattg aatctgcttg aagaaccaac gaagcaagcg
    28981 gttgtcccaa taatccaacc ttatgagatt cggacaatcg gattcgagga ggaaaacgaa
    29041 tgtatggcgg aatagaagca ggcggaacaa aatttgtgtg tgcggtttca gatggtggag
    29101 aaattttaga aaaattaagt atccctacca caacaccaga agaaacattg gatcaggtat
    29161 ttgaattttt tgatcgttat gaattgaagg gaatcgggat tggctcattc ggcccgattg
    29221 gtatcgacaa aacaaaagat aattacggat acgttttggc aactcccaaa aaagggtggg
    29281 cacaattcga ttttcttggc acaatcaagg aacgctacaa tattcctgct gcttggacaa
    29341 cggatgtcaa tgctgctgca tatggggaat tgaagcaagg agcagcacaa ggaaaaaata
    29401 gttgtattta ccttaccgtg ggaacaggaa tcggtgcagg tgtcgtgatc aatgaagaaa
    29461 ttttttcagg gatcgcacat cctgaaatgg gacatatttg ggtgaaacgc catccagatg
    29521 accattatga aggtacttgt ccttatcata aagattgttt agaaggatta gcagcaggac
    29581 cgtctattga ggcacgcaca gggatcaaag ggcaagatct gccgcaagat catcctgttt
    29641 gggctattca ggcttattat attgctcaag cactgatcaa ctatacgttg acgttagcac
    29701 ctgaaaagat tattttaggc ggtggggtaa tgaatcagga tcatctcctt caaaaaatcc
    29761 gtcagcaatt tgttgaattg atgggtggtt atatggaaac acctcaagta agtgaatata
    29821 tcgttcgttg gggactgccg aatgaaagtg ggattatcgg tagtttgctg ttagcagaac
    29881 acgaacatca aaagtaagca gacaaattac acaaaaagaa cagagtctta tcattatctg
    29941 ctgataagac tctgttcttt tttatttcta ttcccataat ccaggtaaag ctttaccgac
    30001 tattttttct tgagtaaatg gatggatcat gtgcagttct aatgcatgga gcatcagtcg
    30061 ttcagctgtt ctattttggt agagagggtc tccaacgatc ggatgtccct tatgagctaa
    30121 atgaactctg atctggtgtg tacgtccggt ttccaaaaca caatagactt gtgtttgttc
    30181 agaaagtact ttgttgacag tgacatgtgt caaggcgctc tgaccatttt tatcatctac
    30241 gacccttttt ctccgatcat ggcgatctcg tccaatttta ttggtgatgg tcatatcttt
    30301 ggcaatttct ccccatacga tggcttgata tctccgatag atcatttttt gttccaacat
    30361 tcttccaagt atcggcaaga caaatggatt ttttgcaaat aaaacggcac cgcttgtttc
    30421 tttgtccaag cggtggacaa cgtaaggtcg ttgctgttta gtgtcgagat aagcagctaa
    30481 atgattgagt aaggtactgt tttctgtcgg ttcatttgga tgagtcttca ttcctgcagg
    30541 cttatccact ataatcaaat gctcatcttc atataaaaca cggatgtgct tcgcatctcc
    30601 taattgaact tcttgataat tataatctgt ttcttcgaat aacaatgtga tctggtcatt
    30661 gccctttact tcatgatgga acaaagcagg ttgttgattg atccaaacgt ttttacgtat
    30721 acgcaaaaag tgtcgaacct ttctaggaac taaccattcc ttttcaagca agtcgcgaat
    30781 tgttgtttca ggatggttca taggtaaagt gatcgtgatt ttcacaagtg tactcctttc
    30841 agtgcaattg cagactaatt gtaccatttt taaaacatat ttaagaaaaa acttacattt
    30901 ttctgttcat tcttatgtta ctctacttgc actaacgtaa aaaaacatat tgaactacaa
    30961 cttaagcagg aagaaaacac tctttcacta tggtaaaata agacgattga atcaaataga
    31021 caggagaaga agcaaatgga ttttcaaagc attttagaaa agataaaagc tgctttgatc
    31081 agtttttggc gttggatcag accatatctc ggacaattcc atcgatggcg aaagcgcatc
    31141 tggaaaaaat atcatgtgaa caaattgatt ttgctgattg gtttgatctg tgtcttagtg
    31201 acaagtatct acctctttat tttagcaaaa caagcaaatg tcgaaacgtt aaaatcagga
    31261 ttaagccaat cgacagtcat ttatgataag aaaaacgaag aagctggcac actgtacgcc
    31321 caaaaaggaa catatgtaga attaaacgcg atttctccct atatccaaga tgcagtaatc
    31381 tcgacagaag ataggaattt ttacgagcat cacgggtttg atatcaaagg gattgcccgt
    31441 gcagcagtcc gaatggtctt gtctggcgga acatcagggg gcggaggaag tacgatcact
    31501 cagcagcttg ccaaaaatgc gtatctgaca ttagaccaaa cgtttgatcg aaaagcgaag
    31561 gaattatttt tggcaatcga gatcgaaaag aagtacagca aagaagaaat cttgaccatg
    31621 tatcttaaca atgcctattt tggcaacggg gtatggggtg tccaagacgc ttccagaaag
    31681 tattttggag tagatgcaac gaatgttacg ctgtctgagg ctgctacttt ggcaggaatg
    31741 ctgaaaggtc ctggtattta taatccgatc gatcacccaa aaaatgcgaa tgatcggaaa
    31801 aacacagtac taagtgtaat ggaagaaaac ggaaaaatca caaaagaaca agaacaagca
    31861 gaaagccaga cagatatcag cgcgtatctt aatgacacat acgataattc agattctggt
    31921 tatcgttatc cttattattt tgatgcagtg atcgatgaag cagttaacga gtatggtctt
    31981 gatgaagaag atgtcatgaa taaaggctat aaaatctata cagctttaga tcaggattat
    32041 caaaagcaat tagaagctac ttatcaaaac agtgcattat tcccagcaaa tgcttcagat
    32101 ggtgccatgg tccaatctgc ttcagtagct ttagatccga atacaggtgg tgtacaagca
    32161 ctagtaggac gaagaggaga tcatgtcttt agaggattca gctttgctac acagatgcgt
    32221 cgttcaccag gatcaacgat caaaccatta agcgtatata caccagcact ggaagctgga
    32281 tacaagcctt ccagtatctt gaaagacgaa ccgcaaagtt attacgatgc gcataatttt
    32341 gacgggacat accaaggaga agtaccgatg tatcaagctg ttgcacaaag tttgaatttg
    32401 cctgctgtat ggcttttgca tgagatcggt ttacaaaaag gatatgataa agcagaagaa
    32461 ttcggcctgc ctttagcaaa atctgataag tattatggct tagcccttgg tggactggaa
    32521 aaaggagtat ctccattgat catggcgggt gcttacagtg catttgccaa tgatggtcag
    32581 atgtatacgc cgcatcttat cacgaaaatc gttgattcga ctggagcggt gatcgtcgat
    32641 aacacaaaca aaaaaccaaa acaggtcata tcaaaggaaa cagccgatga aatgaccagc
    32701 atgcttcttg gtacattttc aaatggaaca gcggctgctg cgaatcctgc aggttatacg
    32761 atagcaggga aaacaggaac cacagaaact aatttcgatg ccacaaaagc aaatgaccaa
    32821 tggatgatcg gttacacgcc agacgtagtg atttcgacat ggatgggtta cgaagaatcc
    32881 agcaagctgc actatttgga agggacaagt ggaaatgttg tcgggaaagt cttcaaatca
    32941 gcggcagaag gcatccttcc gtatacttca aaaacaggat ttagtgtagc agatgcctat
    33001 gccacaggag gacaagtggt agctgcagat caagttggaa acacaggaaa ttctaatgga
    33061 gagtctggaa attggcaaga taacttgaat caatatggtg aacaagcaaa agaagggctg
    33121 aaaaacttcg gcgatatggt gaaagaaggc gttcagggaa ttggtgaagc ggctaaagac
    33181 ctctggcgga aatatcaagg agagtagccc ctttgaaaac gaataacccc atgttacaat
    33241 agagagtaac tactaaaaga aaagagggca taacatgtca aacatctacg atagcgcgaa
    33301 tcaaatcgaa cgtgagatcc gtgaattacc agaattcaaa gcgttgaaag aagcgtacga
    33361 gaaagtacaa gcaaacgaag aagcacacaa actgttcaaa gacttccaag cgatgcaaat
    33421 cgaattacaa gaaaaacaaa tgagtggaca agagttcagc gacgaagatg caatgaaagc
    33481 acaagaaatg gctgtgaaaa tccaatcaga agaagtaatc aatgacttga tgcaaaaaga
    33541 gcaaggcttc agtacaatca tcaatgactt gaaccgtatc atcatgacac cagttcgcga
    33601 cctttacagc gaataaaact attttatgaa acaacagagg gtgtgaaaga gacgttcggc
    33661 cttgagcgat gacaaaattg cttcttattg ccgataggct gtctcttgaa caccatttac
    33721 caagaggtcg tgagcctacc gtttaactct gagttacaag gaacaatttc aaaaaacagc
    33781 ttctcatgtt tgatgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
    33841 attcagaaat aggagctacg acaaaagtct tgtcatagct cctatttttt gtcattcatt
    33901 tttcaagcgg tagaaatagc ctttttgagc taacagtttt tcgtaagtac ctatttcaac
    33961 gatttttcct tcatcgatca caacgatttt atctgcctct ttgatcgttg aaagccgatg
    34021 agccacaata aaagtagtgg gtgtttctgt caggtaattc agggcttgct ggattttttt
    34081 ctctgactgg ttatctaaag ctgaggttgc ttcatctagc aaaatgattt ttggctgacg
    34141 tatcaacgct cgtgcaatgg agatacattg cttttggcca ccagacagct tgttgccatg
    34201 ttcgcctact aaggtatcca gaccatcagg aagctggtca atcagtgaag aaagttgcgc
    34261 agcttcgatg acttcatcta aacgttcttt tgatacgttt ttcaagccat aagtgatgtt
    34321 ctctttgatc gaagcggaga acaacaaagt ggtttgagga acaacggcta aataatttcg
    34381 cacacttcgc atatccagtt catccaaagg ctgatcatct agaaaaattt ttcctgaagt
    34441 cggctggata aatccaatca ataagttaag gagtgtagat tttccagagc cagatggccc
    34501 aacaaaagca attgtttctc cagctttcac ctctagagaa aaatgatcga taatcggttt
    34561 gagtgagtcc ggataaagaa aagaaacatc ctcaaaacga aaatttccat ccaaatgttg
    34621 gatttttttc ttaccgtgat aagtttctac atcgccagca atcaacagtt cgccaataga
    34681 agtgatagat tccatgcctt ttgaaatcgt aggaatcagc gtgatcattc cagaaacgga
    34741 attgacgatc gtcgtgaaat acgtctgata catcacgaca tcgccgggtt ggatgcgccc
    34801 ttgtaaagct aatatcccag tgaaaatcaa acaaatcagc tgaaatatct gaaaactagc
    34861 ccagctgact gatccgaaaa cagattgcag gatatctaag tggaatcctt tttcatagat
    34921 ttgagtaaaa tgcttattta agcgagttgt ttcgtgtttt tctaacccat gagctcgagc
    34981 aatcggtgct aattcgatca cttcgatcac ttttgcagaa gtctcttcca tttcttgcct
    35041 gaagtctgag ttggactgtc tgatcctttt acggaaaaag agagtaacga atgcggcgag
    35101 aggaacagtc aataagaaaa acatgaaaac aacaggactt ttataaagcg tgatacctaa
    35161 agctacgaat aaattgacag cgatatttaa tccgctgaca aatacttgag aggacaacgt
    35221 ttggatagct tctacatcac ggatgatctt tgactggatt cgtccagatt gcaattcttt
    35281 ttgataagga atagatagtt cttggatttt cttgataagg gtcgctctta gtccagcttc
    35341 tacttgacgg atcgaagtgc tatggtattt tacatgaaaa taattcgtca atatattttg
    35401 accaatcaga caagccatca aaaaagcgtt gaaataaatg gcctgcaact gagaagaatc
    35461 tttcgatgaa gctgcattga ttacattggc aatagcgatc ggcattaacc aagatggaag
    35521 atgtttgata ataaaaaaga gtcctgacat ggcaaaacgc caataatttc cctgataaag
    35581 atgcagcaat gtctgaaaag catttgcatg atatttttga taacttttct gtattcgttc
    35641 ctccataatc ttctccttaa caatcttggt aaataaactg caagtgttct attgcttcag
    35701 gaacataaat ctcaggagta gcttcaagag tggcgtacat tagaggacgc tcatatttga
    35761 tataatgcag taccgggcgg aaatctaaac agcccttgcc aacaggtacc atgcggatct
    35821 ttccattttc catgatatag tctttgagat gaagcgctgt aatataacca tgtaaattgt
    35881 ccaaggcacc tcggatcact ttttcctgat cagcatgatt ttctttatgc atcaaattcg
    35941 cacaatctaa aatgattttt aaatttggtg aagctacttc atcgattaaa cgtttagcta
    36001 actgatagtt atagattgga tgattgatcc ctgcttcaac agcaacagtt acgccaaaat
    36061 tttctgcatg agcgactaat tcaatgatag aatttctggc gatctgataa gcttcttcag
    36121 taaaattttc ttttgtgtaa ccgacagaga cactaccggt ttccgtagca atcatcttgg
    36181 cttgataggc agagcacaga gcaatttgct gtttgaaagt tgccaatgct tgtgcacgaa
    36241 cttcttgatt tttgctggat aggttgatat agcatcctaa aatggaaagt tcgatattgt
    36301 ttttttggaa gtattcacca aaataggcag cactcccaag tgtcatgtca gttaatttaa
    36361 gttgttcagg aaatgctttt tgtggtgcaa attggatatg ggacaagtga agctgtgata
    36421 ctttttgaac gatttcttca cgatttcgaa catctaaatc gtgggcacgg atgcctaaat
    36481 ttaatggaaa catctcaaac tcctttttgt aaataattag caagaggcag acgggattct
    36541 tttatcaatt caacaattat tcgggcaact ttatcagcgc cttctggact aaagtgggta
    36601 ttgtctttta gtccttctgg gtaattttta caagtatcag gcataagatg caggtagaat
    36661 ttttttgtct cttcttttga gaaggtctgg aacaattctt gtgatcttga gaacacatct
    36721 aaaagaggaa tatggtgttt ctcagcaaat gcacgcatcg cttttggata gtctccaagt
    36781 atatctggat tcaatgttcc gttttctaag taatctcgtc gcgtcacaga tgtcagtaag
    36841 accatttgga cgttcgcttt ttttgcgcat tcaaaaaata tctgcagatt ttttaaatag
    36901 ctaccgaagg gaagcgtacc tctttcttct tggattttct ggtcgttatg tccaaattgt
    36961 acaaataaga aatcattcgg ctgaaactgt gaagctagac gggctaacct tccttcctgg
    37021 ataaatgatt tggtgctgcg tccattcttt gcttggttat tgatctggat ctcgggtgaa
    37081 aaataagcag agattttttc tccccaacct gtctcgggac gcttgttttc atctttttct
    37141 gccgcagtag aatcaccggc aatatggata gtaggcatgt tatctcttct ttcataaagg
    37201 tttataggca atgaagcaga acagcaagag aagaagacgg agcaa
//
LOCUS       DAJSAW010000027        32177 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJSAW010000027 DAJSAW010000000
VERSION     DAJSAW010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.027"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(408..767)
                     /locus_tag="ONT64_001457"
     CDS             complement(408..767)
                     /locus_tag="ONT64_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR2302881.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(797..1138)
                     /locus_tag="ONT64_001458"
     CDS             complement(797..1138)
                     /locus_tag="ONT64_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302882.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1135..1809)
                     /locus_tag="ONT64_001459"
     CDS             complement(1135..1809)
                     /locus_tag="ONT64_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR2302883.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2985..4283)
                     /gene="asnS"
                     /locus_tag="ONT64_001460"
     CDS             complement(2985..4283)
                     /gene="asnS"
                     /locus_tag="ONT64_001460"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR2302884.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4323..5513)
                     /locus_tag="ONT64_001461"
     CDS             complement(4323..5513)
                     /locus_tag="ONT64_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR2302885.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5534..6061)
                     /locus_tag="ONT64_001462"
     CDS             complement(5534..6061)
                     /locus_tag="ONT64_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HCR2302886.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6108..8897)
                     /locus_tag="ONT64_001463"
     CDS             complement(6108..8897)
                     /locus_tag="ONT64_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR2302887.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9044..9244)
                     /locus_tag="ONT64_001464"
     CDS             complement(9044..9244)
                     /locus_tag="ONT64_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2302888.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9697..10017)
                     /locus_tag="ONT64_001465"
     CDS             complement(9697..10017)
                     /locus_tag="ONT64_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302889.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10520..11638)
                     /locus_tag="ONT64_001466"
     CDS             complement(10520..11638)
                     /locus_tag="ONT64_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302890.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12109..13416
                     /locus_tag="ONT64_001467"
     CDS             12109..13416
                     /locus_tag="ONT64_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCR2302891.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13537..14736
                     /locus_tag="ONT64_001468"
     CDS             13537..14736
                     /locus_tag="ONT64_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR2302892.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14763..16031)
                     /locus_tag="ONT64_001469"
     CDS             complement(14763..16031)
                     /locus_tag="ONT64_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HCR2302893.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16201..16842)
                     /locus_tag="ONT64_001470"
     CDS             complement(16201..16842)
                     /locus_tag="ONT64_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HCR2302894.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17251..17754)
                     /locus_tag="ONT64_001471"
     CDS             complement(17251..17754)
                     /locus_tag="ONT64_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR2302895.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17877..18641)
                     /locus_tag="ONT64_001472"
     CDS             complement(17877..18641)
                     /locus_tag="ONT64_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCR2302896.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18749..19024
                     /locus_tag="ONT64_001473"
     CDS             18749..19024
                     /locus_tag="ONT64_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR2302897.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19584..20534
                     /gene="yidC"
                     /locus_tag="ONT64_001474"
     CDS             19584..20534
                     /gene="yidC"
                     /locus_tag="ONT64_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR2302898.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20580..20981)
                     /locus_tag="ONT64_001475"
     CDS             complement(20580..20981)
                     /locus_tag="ONT64_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR2302899.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20995..21804)
                     /locus_tag="ONT64_001476"
     CDS             complement(20995..21804)
                     /locus_tag="ONT64_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2302900.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFVALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21791..22681)
                     /locus_tag="ONT64_001477"
     CDS             complement(21791..22681)
                     /locus_tag="ONT64_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2302901.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22694..23173)
                     /locus_tag="ONT64_001478"
     CDS             complement(22694..23173)
                     /locus_tag="ONT64_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2302902.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNISRIMVVDDEVATNTIEK
                     SGLKLATPAGVKLSILPIEKAATNILAGKYDSQRVLIVAKKPDRILSLVEKGVPIKEV
                     NVGNMSQTNETRSITKSINVVDADIEVFKKLDQLGIHLIAQMVPNDKAEDFMNLLIK"
     gene            complement(23349..24290)
                     /locus_tag="ONT64_001479"
     CDS             complement(23349..24290)
                     /locus_tag="ONT64_001479"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HCR2302903.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24287..25282)
                     /gene="lacD"
                     /locus_tag="ONT64_001480"
     CDS             complement(24287..25282)
                     /gene="lacD"
                     /locus_tag="ONT64_001480"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCR2302904.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25275..26471)
                     /locus_tag="ONT64_001481"
     CDS             complement(25275..26471)
                     /locus_tag="ONT64_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR2302905.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26489..27217)
                     /locus_tag="ONT64_001482"
     CDS             complement(26489..27217)
                     /locus_tag="ONT64_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2302906.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27658..28632)
                     /gene="lacD"
                     /locus_tag="ONT64_001483"
     CDS             complement(27658..28632)
                     /gene="lacD"
                     /locus_tag="ONT64_001483"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCR2302907.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28629..29087)
                     /locus_tag="ONT64_001484"
     CDS             complement(28629..29087)
                     /locus_tag="ONT64_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2302908.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29084..30547)
                     /locus_tag="ONT64_001485"
     CDS             complement(29084..30547)
                     /locus_tag="ONT64_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2302909.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30529..31461)
                     /gene="pfkB"
                     /locus_tag="ONT64_001486"
     CDS             complement(30529..31461)
                     /gene="pfkB"
                     /locus_tag="ONT64_001486"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR2302910.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31603..>32177
                     /locus_tag="ONT64_001487"
     CDS             31603..>32177
                     /locus_tag="ONT64_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2302911.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFF"
BASE COUNT         9384 a         6920 c         5511 g        10362 t
ORIGIN      
        1 tttcttccat acagatgttt tggcttattc tttctttttc tcttcgatca ttgcccctgc
       61 tcttatccaa cccgcacttg ccttcttgat cttgacagga tagcaactaa gtttgaagcc
      121 tgttgctggc aatttgtcta aattcattaa tttttcgata ggacaatatc ttttttcaat
      181 ccctgcaaaa tgtccttccc agatattcga agctccacta cgctttcaaa aatatagaaa
      241 gagttcttta atgaaaatta tttcaacttt ccataaacag taatcccata taaataaaag
      301 acgtaaaagt cacatgggag aacttataca ggaaaacaca tagagtcagg tgaaatacat
      361 gtatcccacc tgactctatg cgttttctaa cctttattat tctcttttta tacaatttct
      421 gcaccgaatg gcatcaacgc caatcgagca agtttgaaag attgcttggc aaaaggtatc
      481 cctatgatcg tgatacatag aaggatgccg ctgacgacat gtgcgagagc aattggtagg
      541 cctgaaacga tcagccagat gatattaacg atcaatgaca tgccagactg gtgataaatt
      601 acttctttgc caaaaggcgc aaaagaaagg gaggcgatct taaaacattg cataccgatt
      661 gggataccta tgatcgtgat acaccaaagt atccccgctg ctaaccagct aagaccaccg
      721 attagtccgc cgaatataaa ccagatgaca tttcctaaac aactcattgt atttctcctt
      781 ctattactgt ttttgattaa aactgggcaa aaattgattt tattcttctt tttttagctt
      841 ccagcatcaa gtattctgca aatttttttt ggatttcttc cgtattttct tttgttacta
      901 atgtgtgttg gattagtttt cctgatggtc gctgatccac ccgttcttct gactgttgca
      961 cgtccgcttg cgcaaatccg ataatccgtt tttcttttgt tacagcaacg agcaatagtt
     1021 tattcttatc atttaggtca gctgccgttt ttgcttttac gaatgattct ggatgatcag
     1081 taatcagctg attgatttga ggtaaatctt cttttactgc ccaccgtatt tccatcattt
     1141 ctcacttttc tttcgctcat ttttatctcc actaaaaaaa tcgactgcgg caattgcatc
     1201 tatccagctt tgtgcaatga tctcatgtcc gccaatcgtt gggtggaccc catcttctct
     1261 tgtatatttc gaaaaaccat aagcaatccc taaagcattg aatatgccat ctaaaggaac
     1321 cagcaatgcg ccatattcct tagcaagttt acgaacaatt tggattttag ggtctaagtc
     1381 tttacgccag ccatgacggt cgtcggttat aggaagtaca aacggctcca tcaagatgat
     1441 gttgcagatt tccgctgttt gaagttcatt tagtaatttt cgataaacgg attcaaattg
     1501 ctgcgcagat ttctcagttc cgaatatagc ctgatcgcta gtattatgcc aggtatcatt
     1561 gattccgatc aaaatcgata catagtcagg ttgtaaagct aaacaatctt cttgccagcg
     1621 tacttgcaga tctgcaattt tgtctcctcc tatcccacgg ttcatgcatt caaatttcat
     1681 cgttggatat tgggagatta gttttccggc aatcaaaagt ggataacctt tacctagatc
     1741 agttgcttct gctcggtctc ttcctgcgtc tgtaatgctg tcaccgataa ataatatacg
     1801 ttttttcatt tgtcaaaaac tcctttttga ataaaacgat gattttcttc cttatattat
     1861 cccataagcc ctatcaagac gaaagagaaa atatatgtat aaaaaatgac accagcctag
     1921 tcccttcaat caattgaatg aacgctcatc aagtgtctag ttttttatac tttttttctt
     1981 aacgtgccgg attccccttg attccttcgt tgtttggtcc agagaaatcg atcacaccta
     2041 agcttccgtc aatcagacca ctgatgacgc catcagaaca tatatttata ggctcatcta
     2101 agacttccag taaatgttct tttactaata tctcttctag attgcttggg atagattggt
     2161 cataacttta tctggtccga tgatttcaag gatgaattaa acttacatat ggatcattgc
     2221 atgctgaaaa tacggaccta ctccatcttc gaatgttact attagtttgt tttcaggtac
     2281 tagatgctgc tttatttcaa cagcagaagc atcatcaaat cagatttcca tttgaataac
     2341 cttctttgta tatgtttgat tgattgcgat ttcgttatag aaccttgtga atcaaatagc
     2401 aatttctttt gctttcttct cttgtctgct ggaaacttcc tttatcccgt attttaaaaa
     2461 aagaactgcg agagttcgca gttctttttt ttaaataaac gttgctctcc ggccaactcc
     2521 tcggtcacaa gtcacaatag tcatcaaaca tgaaaaactg ttttttgaaa ttactccttg
     2581 tgactcggag ctgaacggca ggcgcacaac ctcttgatat aaacggtgtt caatcagctg
     2641 accctcgata ttagctaaaa aaacggtaaa atatgagaag ctatttttcc gttttgtttc
     2701 tctaatactc aggtcaaggc actgatttca caacctcttc cttaaacggt gttcaaaagc
     2761 cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg aaagccattt ccacaaaatt
     2821 tttcttaata ctcgaaacta aacagctttt tcacaacctc tccactaaac ggtattcaaa
     2881 agcttacatc tgcggtaata agtcccgcca attcaaaaac atgaacagct gtttttgctt
     2941 ggctgttcta attacttgat gcagaacagc tttttcatca cctcttatgg ataaattcgg
     3001 ttcaacaaac gtgggaatgg aatagcttca cgtacatggt cgataccagc aatccaagtg
     3061 actgttcgtt caaggcctag accaaaacca gagtgcggaa cagaaccgta tttacgtaga
     3121 tcaagatacc atgaatattc tttttcatcc aaaccgtgat tacggatttg ttctagcaag
     3181 aaatcatagt ctgttgcccg ttcgcttccg ccaataattt caccgtaacc ttctggtgcg
     3241 atcatatctg cacaaattac cacgtcttct cttgttggat gtggtttcat gtagaatggc
     3301 ttgatggctt ttggatagtt taggataaag acaggctgtt cgaatgaatt cgcaatgaac
     3361 gtttcatgag gtgaaccaaa gtcttcgccc cattcgatgt catcaaatcc atttttcttc
     3421 agtaattcga ttgcatcatc gtaagtaata cgagggaatg gcaattttgt ataagttttt
     3481 aagacttctt tgtctcgtcc tagtacatct aatgcatatt cgcaatgatc taaaacattt
     3541 tgtactaaga atgctacata ttgttcctgt acttctaaac tttcttcttg atgagtgaaa
     3601 gccatttctg gttcgatcat ccagaattcg atcaaatgac gtcgtgtttt tgatttttca
     3661 gcacggaaag tcggaccgaa tgaaaagact tttccgaaag ccatagcagc agcttccatg
     3721 tataattggc cactttgaga taaataagct ttttgatcaa agtaatttgt ttcgaataga
     3781 tccgtagtgc cttctggtgc agaaccagtc aagatcggcg gatcaatttt gacaaaatga
     3841 tgttgattaa agaattcata cgttgcacga atgatttcat ttctgatctg cataatcgca
     3901 tgttgtcttg aagaacgcag ccataaatga cgatgatcca tcaagaagtc tgttccgtgt
     3961 tcttttggtg tgatcggata atcgtggctc tcgcttacca cttccacatt tgtaattccg
     4021 atttcataac caaattttga acgactgtct tcacggattt ctccagttac taacacggat
     4081 gtttcttggt gtagattttt agctgtttgg aaaacttctt ccgacacttc gctttttaca
     4141 actacacctt ggaaaaaggc tgttccgtct cttaattgta aaaatgcgat ttttccgctt
     4201 gatcgcttat ttgcgaccca agcgccgatt gttactgttt ctcccacatg gtttttcgaa
     4261 tcgatgattt gaatttgttc cacgtatgac tctcctttta tattccttga atttcttcag
     4321 gattaatttt gacttttact ctcaataaat tgcttgattc tgcgtaccgc ttcttctaac
     4381 gtagcgcgat cagtggcata actcatgcgg acattttctg gtgccccaaa gccttcgcca
     4441 gtcacaagcg ctacccctgt ttcttctaaa agatcttcta cccattgtgt gacattttca
     4501 tacttacaca tgcgtagcgt ttcttttaca ttaggaaaaa gatagaaagc tccttgcggc
     4561 ttttcaagag aaacacccgg cagttctgcc acaagcggat ataacgtatt caggcgttct
     4621 tcaaaggcgt tccgcatttc ttctactgta tcttgctcac ctttcaatgc ttcaacagct
     4681 gcatattgac tgactgctgt cggattgcta gttgattggg aagcaacagc gatcattcca
     4741 ttgatgatca caggatttcc caccgcataa ccaatgcgcc agcctgtcat cgcatatgtt
     4801 ttagacacgc cgttgatgat gatggtctga tttttgattg cttctgaaat cgttgcaatt
     4861 ggagtaaact cattgccatt ataaactaaa cggccataaa tatcatcagc aactatcaaa
     4921 atatcatttt ttactgccca ttcaccgatt gcctgcagtt cattcttgct ataaatcatt
     4981 ccagtcggat tcgatggaga attgatgatt acggcttttg ttttttctga acgtgcttgt
     5041 tctagctgtt caacagtaac tttccagcta ttggattctt cccctttcac aaagacagga
     5101 agtccttctg ccagttttac ttgctcaccg taactgaccc agtaaggcac aggaatgatg
     5161 acttcatctt gcgggtcaag gatcgtttgg aaaagggtat acaaagcaaa tttcgctccg
     5221 tctgttacga ctgtttgtga aggttcatag acaagtccat agtttttctt cagataatca
     5281 acgattgcct gtcgaagctc tgggattcct gctgttggtg tataatagct agctttccca
     5341 ttgcggattg cttcgattgc tgcttcttgg atgttttctg gtgtcgcaaa atccggttct
     5401 cccacagtca aactcagcac atctctgcct tttgctttta atgccttggc ctttgccgca
     5461 gctgccaaag tgacagacgg ttctaatcgt tttgttcttt ttgataactc catcccattc
     5521 attcctttac cttttaaatt cccttgattg tcttgacttc atctccagat tcaaagccaa
     5581 tcaggtaata attcagtttc ccatttgttt cttttcctac tacttcccac acaggctgat
     5641 catcatacat ccctaaagca gctttttcga tttggatttc aggatgggcc gcagcaattg
     5701 tctgttttgc ttcgttttca gtcagtccct ttgtttgatc caatactttt actttttcac
     5761 cagatttagg aatgataacg gcgatttctt ttcctttatc atttgtacct gtcagtgaaa
     5821 agtatgtctt ctttcgagta aaccaataaa aatgatcggt ttcctcaaga tttgcatatt
     5881 tctttgcgat ttctactgct tctttcttcg cctgcaccgc cggacgactg gaacgcaagt
     5941 aaaaaatcga gctgatcacg atgaccgcta ataaaaatac gatcaagcca agtaacacgc
     6001 gattaatcgt tttttccttt gtctgttttt tcttcaaggg tcgtcgctcc tttatcttca
     6061 ttccttttca ttatagcaga ttttaattat gaaaaaagac agctcctcta acttccttct
     6121 tcatttttct ttaagaattc atggatatcc tgatagattt cctccatagc tagctctttg
     6181 atcggtactt ttttcggtaa tgcatggatg atccgtgcgc catattttgc agtgactagt
     6241 cttcgatcca aaataagcaa tacgccacga tctgtctccg tccttatcaa gcgacctaat
     6301 gcttggcgta acttcaaagc tgctttaggt acggattcct ggtaaaaagg attgatcccc
     6361 tctgattcta aataagcgtt cctagctttg accatcgggc gaagcggatt atcgaagggt
     6421 aaacgtgtga ctacaacgat ctgcaaagca tctccaggca aatctacacc ttcccaaaag
     6481 ctgtcagcgc caaacaatac gcttttttct gacaaaatga agcgtttcaa aagcttctcc
     6541 cggcttccac caaacccttg agctagtatt tctcgtcctt tttctagcat agacagatgc
     6601 attttctgat agaccttctg caaaacatca tgagaagtga ataacacaag tattggccga
     6661 tcttcttgtt cgatcagacc tgtaagtgct gtctctaaat aatcgatata tgcttgattg
     6721 ctgacttgat ggacggcagg tgcctctttt ggcacataga tccttgcctg tttctcataa
     6781 tcataaggag aggcaatcac tttaagaggt gctgcttcaa tgccaagcct cttagagaga
     6841 tagtgtcgat tagggccgat cttcagcgtt cctccaatat aaagaatacg atcatatcga
     6901 ggataccaaa cagtcgacgg caacatagcc gcttgaaaat catggagatg aaaaatcatc
     6961 tgctgcgctg tgttcccagg aacgaaccaa tggatcaaat gatctcgcca gtcttccagc
     7021 cattgatcca tcagcaagta ttgcttgtct aattgatcaa ataggctata taaacttgct
     7081 aaatcgatcc gcatctggtt tgtccagctt tcttggataa tctcaaaagt cagacgcatt
     7141 tgtgactgta gctgctggat ctcctgataa aaaagctgca ggtgatcaat ggctgtccca
     7201 gattccagtg gcagctgatc caacatctct tttgtcatca tgacttcttc tggatactgt
     7261 ccgttaggtt gcttgccgct cactaaactc attaatcctt ctgccataca aatctgtgct
     7321 tcgcatagcg cagaaagttc ttctcgataa atcgataacc agcgtaaggc ttcatgttct
     7381 ttcggtaaat agctttgtat tcggtcaaac agctgatttg gttcttctaa ttgctggatt
     7441 tgtttatgga aatagttaat acttaatcgt ttgtcattta ctttttcagc gatttccggc
     7501 aaatgatgta cttcatcgat caacaaaaat ggactagctg gaagcaatgg ttcttttcgc
     7561 tgtgtttctt gtactaaaaa cgcatgattg acaatcaaaa aattactttg cctgattttc
     7621 ttttgcaaga aacgaacaaa gtcttgttcg taaaatgact gatttcttgc taaaaatcca
     7681 atacctctgt gcgcgatatc tgcaaagaat gaatggttca aatttgttaa atgtaattca
     7741 tccaaatcac cagtcttcgt ttgcgtcagc caaactaaga ccgccatttg atacaaagca
     7801 tattgttttt gttctggcgg ttccacaagt gtgcctttaa aacgctgcaa atcaatatag
     7861 tggcgatagc ttttgataat tacaggttgt atgggctgat ctagcaattg attcagcaat
     7921 ggaatgtctt tttctatgat ctgctgctgc aaaactagcg aaacggtact gatgacagcg
     7981 ggtttttcag gtgtagccaa ataactcaat ggcagcagat aaccgattgt tttacccatt
     8041 cctgtagctg cttcgatcat caaatctttt gattcatctt ttgtgaaatg gtcgtagacc
     8101 gcattcatca gtctgttctg ttcttttcga tacatcagtg ttttaccaaa cattttttct
     8161 tttgcttgtt tttttcttgg atatgtccgc tcgccataat ggacggatgt gaatagttcc
     8221 acttcttttt tctgcaaagc tagcccatcg acgatttcta atgagggatc aagtggctct
     8281 ggtttttctt tcatttcttc aatcacatga taaatgaaac gactcgtatc catacctgtg
     8341 tgtctactta atcgcgcaat ctgctctaac gtgatgattg gcaattttct catccgttct
     8401 tcgatcagca aaagaagttg acctgtcact tcggcatcac tatctgcctg atgaggattc
     8461 tcatgaataa atccaaggct ttctgacaaa tcagctaagc gaaagcttgg ttccgtcggc
     8521 aagaaaattt gtgcaagttc tactgtatcg atgcctggta tcttcaaact aggaagtccg
     8581 catcgcatga gttcatggtt caaaaaatta taatcaaaat aaatattatg cgcgacaaat
     8641 atggtgtctg ccaaaagatt gtaaatcgtt tgggcgacat cttcgaaata aggcgctttt
     8701 tggacgcgtt gattcgtgat ccctgttaaa tgctggattt gttttgaaat cttccgccct
     8761 ggatgaatat cgatcgaaaa acgtgaagtg atccgacctg attcaatcat gacgcagcca
     8821 aattggatga tccgatcttc ttttggattc gtcccagtcg tttcgatatc aacgactgca
     8881 taagttcttg gtttcaaaat atcttcctct tctcctgcct ctaatgttct tttgaaatta
     8941 tactacaaag tgcagaaaaa tactctcatc gctttttaaa gaaaatagac aaaaacgccc
     9001 agacacgcaa atgtctgggc gtttattttt tagtataggt taattaagct ttagtaacgt
     9061 ttgttgcttg aggtccgcgt tggccttctt caacatcgaa agttactgct tggccttctt
     9121 ctaaagtttt gaatccgtcg ccttggatag ctgagaaatg aacgaataca tcgtttccgt
     9181 tttctgcagt gatgaatcca aatccttttt ctgagttaaa ccattttact gtacctgttt
     9241 ccataataaa aatcctcctt gtgtatcaac acatattttg caattacttg aatggtgtat
     9301 atctggaaga tgtttatttg aaacaagcta ccacagatta ccgaacaaaa actactccta
     9361 tagtatatca taccgtttac aaaaaagcta gagtttttcg acttttttta ttaaaaaaag
     9421 ttttctcagc gattacataa agtgaaaaac cgaacttttt tttaaaataa acgacatatt
     9481 gttcagttgg tgtttttttg ctctgatgtt cttttttaaa ctaaaaaaat tattctttct
     9541 gactacttat cggttctgtt tcaacggtcg aggctgggga agattcttta gtaggtctga
     9601 tgttctcatg gtatttctga tagcctatga cagttaccag aagaacaagg caaaccaata
     9661 cgaccaacga gattagtttt tcttttttgc tcatatctat acctcataaa taacaggatg
     9721 atgtccatca tatttctgag tatgagtaaa agcaaaagct gcagctgtag aatgtaagaa
     9781 atgccagcaa ctttcataat cacaggtcgg acagtaccaa aagtaaccaa atcttggctg
     9841 caaagaccgt tcttctgttt caggaatcgc agaatcaaga tctggtgtct gtacataaac
     9901 atttttgtac agacgtgtgc ctgtaatagg aaaccgctgt gtttcttcag caaaaactgg
     9961 ctgacttgtt aaacttaggg atagagacag caaacaccat aacagtcttt ttttcataaa
    10021 gacctcctta tcgataagtt tttcccaatt aaaatcatat ctcaagaacg cccgttattc
    10081 acattttgtt cataaatgat gttttttgta tcataatcgt caaaaagagg tcgtgaccac
    10141 gtcacaacct cttttttaaa cggtgttcca gaagtagctt cttcggaaat aagccgaaat
    10201 ttcacaaaaa tttgagaaat aatttttgtc aatctcttct tatttcttga agctgaccac
    10261 ttctgtcaca acctcttcct taaacggtgt tcaaaagctt gcatctgcgg taataagccc
    10321 accaaacaaa aaatatgaag agctattttt gcttgctgtt cttattactt gatgcagaac
    10381 agctttttca caacctctca tacacggtgt ttaatcagct gaccttcggt attagctcaa
    10441 aaaacggcaa aatatgtgaa actatttttc cgtttgtttc tctaatactc aggtcaaaac
    10501 actgatttca caacctcttt taataacgaa gaatcgctgt cagactcttt tcatctggcg
    10561 catctttttg atccaaaata aacacttttc cgccattaat gatcacatca tctgctaatg
    10621 cattcaacac ttgacgccga tcatattctg tttcaggatc ttcaccgaaa ccatcagcta
    10681 agtttgacgt agcgataaat aaatgtgaca cttttccttc tttcgcagct ggaacgatat
    10741 caactaactg atcgatgaat ttcttatcta agagtttatt gtattctttt gattcttgag
    10801 ctgcgagttc acttgacagc ttcttcgccc cttcttcgat atctctaaag gatagctgcg
    10861 caggagaagc agaaatcgca acgtctgcac aataataggg atttttagca atttttttga
    10921 acatcgtctg attttctggt agagcaaata aataaatcgg ccattgttct ggattttcga
    10981 aagaatcctt caagtacgta tcaactgctt ggtaatagtt gacccaatcg atctctactt
    11041 cttcatcttt tggatttacg ccatggaaag ttactccatc tcgggctccg cctcctccat
    11101 tagaagagaa gttcaaaccg ccacctgtca gttcagatcc caatgcagta acgacatcaa
    11161 ccggtgcccc ttctggtaga gatacttctt ttacttgttt gttgctgact tcatacaatt
    11221 tcatggagtc gcggttcaat cccaataaat aataagaata gttgaactgc ccatttttga
    11281 taatagccag taaataagga agatcactta catagtattg atcgtctacc gggatactca
    11341 aacgatggat ataggtttgg tcagcagtta taatgaccgc tacactcgta gttgcactac
    11401 gccaaaacga aggatctcct aacaaatttt caattttttc ttggaacggg gaccagtcat
    11461 gttcggaata ttttttctca aaacgtttct ttgcttcttt cgcaaaattt ttgaatttga
    11521 tctgatcttt ttccacatct tgatgggcga catgcgtatt cagcatgatc gtgacgaatg
    11581 gtcctttagc gccttctgaa gtcaattgtg acaataattc tttatccttt tttgccataa
    11641 acgtacccct cctaaaaaaa tcattccttt gtttctatct aaagtctacc acaacactat
    11701 ttttccatac aataaaacgt atctaaaaaa ataaaacggt ttctacggtt ttaaatttag
    11761 ctattttttt aagttatctt cataatttcc tcaattattt agccccagtc ttcaactatt
    11821 cttcatgaaa tgctgataaa ctaagaaaaa gaataaaaga ggtggatctt atgcatttga
    11881 ttatgatttt cagtttatta agtattatca gtttatttgc tttcagcgct tcccgcttat
    11941 tagatgcgga aacacagcct attcctgtaa aagtacgtaa aaacgaccgc taaccttcca
    12001 taacgtgcgt tcttatccta aagttttttc agaaacattg aaactctctc ccctctctga
    12061 taaaatttta atttgtagcg aaacaaatta attacaagga gatcggatat gtccataaaa
    12121 aaaacacgtc tagtcctatt attggctagt ctcattcttt gccttgcttc ttttctacca
    12181 gctgttgctg tgcaagctga agatactttc aaagtaaatg caaaagcagc ctttgctgta
    12241 gatgcgcaaa gcggtaagat tctttatgat caagatggag aaaaaaccat ggggatcgct
    12301 tcaatcacaa aaatcatcgg tctttacatc gtccttgatc aagtaaaaga agggaaacta
    12361 tcatgggatg ataaagtttc aatttcagat tatgcagaaa atttgagtgt caccccagat
    12421 ttatcaaatg ttcctttgca taaagaaaac acctacaccg ttaaagaatt attcgactct
    12481 gcaatcatcc agtcagccaa cgcttcaatg gttgccttgg cagaaaaaat ctctggcagc
    12541 gaagcaaaat tcactgaacg aatgaaagaa caattgaaag attggggtat caaagatgca
    12601 acgatcgtta atgcatcagg actgaataac tcttatcttg gcgaaaaccg accagaaggt
    12661 actggagaaa acgacgaaaa tcaaatgtct gctcaagatg tggctatcgt tgcccgtcat
    12721 ttgatcttgg attttccaga aatactggat gtatctagca ctacgacaca aatgtttggc
    12781 gaaaacacgc aatcaccagt agaaatggtc aactggaact ggatgctccc aggattcgtg
    12841 aattataaag aaggcgtgga cggattaaaa actggtacga ctgaacttgc aggagcctgt
    12901 ttcgtcggta cgatcactaa aaacgatcaa cgaatcatca ctgtcgtatt gaatgcagac
    12961 ggccatgcgg aaaacccaag tgcccgtttc aatgaaacag ctcgcttaat ggattattgc
    13021 tacgacaatt ggtcagaaaa agagcttgga aaagccaatg caagtatccc tagtctaaaa
    13081 accattgatg taaaagacgg aaaagaatca acagtgcccg ttgtgcttaa aagtcctgta
    13141 aaagtatggg tacggaacga tatggatact ggaaaattaa caatcacacc aactcttgac
    13201 aaaaaacaag tcactgacaa tgagatccaa gcacctatgg ataaaggaac aaaaatcggt
    13261 accgcaacga tcaccttggc agaagataaa ttaggttatc ttgaagacaa caacgcgcca
    13321 tccacagaaa tcatcacgga taaaaccgta gaaaaagcaa atattttcgt tattggctgg
    13381 cgacgcgtcg cagaattttt cggtaacttg ttttaattga acaaaaagaa aaaagcatct
    13441 ttccatgtgg aagatgtttt tttgcttttt ctagtttaaa taacagcttt tttcatctat
    13501 tttcgttata ctgtataaga aaaaggagtt gaatttatga atctaaccag agatctgatt
    13561 tttttggttc tctatcttat tggggtattc tccatcgttt attggcgaaa acagaaaaag
    13621 gatccttcct tgttctttta cagcagtaca tggacgtttg cggtaatctt gacttatttt
    13681 gtcgtgttgg aaatttttta ttcgaccttt tattttttga ttcccttagt gtttttcagt
    13741 atctttgcct tttcctattt ttcagaaaag cgaaggctgt tgaatggtct tttattcaat
    13801 attttcctca ttagttttgg tatctacttg tttgtcttgc tatacgaaac acaaaatatt
    13861 tttcttggcg gactgattgc cttgatcacg atcccgttac tgcttgtctt tctattcggc
    13921 atttatggat tgatcgtttt tcttttttgg aacggtgtca cagttttacg tagagagtcg
    13981 cattcattag ctaatctttt aacgttgata ctagccattt ttcttacatt gtttcttgtt
    14041 tttgactttt tcttgttaaa atatttacca caatggataa atgctttgtt tttctgcatc
    14101 ccattgatct tgatctatct atttatcgta ttctacaact ttttgactgt ctcattcttg
    14161 tatcagttca accgccctcg atacaaccaa gacttcatcg ttgtgttagg cgccgggttg
    14221 atcaatggag aaacagtttc tcctcttctt gccaagcgaa tcaataaggc gattgctttt
    14281 tattgggcac aaagccgtgc gacattaaat ccgcctatcc tattaatgtc gggtggacag
    14341 ggagcagacg aaaaagtacc agaagcaatg gcgatgaaac aatatgccat ggaacaagga
    14401 attcctgaaa gagatattct tgtcgaaacc aattcgacaa ccactttgga gaatatgcgc
    14461 tattcaaagg aaatcatgga tcagcaaatg aaaggtccct atcgcgcgat cttcagttcc
    14521 aacaattacc acattttcag agctggtctc tatgcacgac aagcgaaact gaaggcaaat
    14581 ggcattgggg caaaaacagc tttctattac ttgcctaacg cgtttttaag agaatacatc
    14641 gccatcttga tgatgaacaa aaaaagacat atgatcattt gcggattgct tttcgcaggt
    14701 tccgcattct tatcactgat cactttactt ttctaaaaaa acaaggtgtg acaaaacata
    14761 tgtcacacct tgtttttttc ttttattctc ttgacgattg gcaagaatag tattcctttt
    14821 ttgacggcta cttgctgtgc agaataaata ccgctgtttc ctgaacacat aaagctgatc
    14881 aagcagatca taaagaagta agcagcagct tgtgaaccaa ataattcgat tcccatcaca
    14941 aaacaggcaa tcggtgtatt tgtcgcacca gaaaataccc cgataaaacc taatccagca
    15001 agaaaaggaa cagaaacatg gagcaaaggc gctagtactg cgccaagcgt tgcaccaatc
    15061 tcaaatagtg gtgtcacttc tcccccttgg taccccgcac ctaaagacaa gaccgtgaag
    15121 aataatttcc caataaaatc ataagcatgt gcctgtccat taaatgattc ctgcagtaac
    15181 ggaagactca acccaaggta acgttgtgtt tgcaaaatca acgctgcggc tacaactact
    15241 gctccaccta tcgtatttcg ccaaacagga tgaggaatcc attgcgcata ccgcgctttt
    15301 aaaaagacga tcgaacggct gaatacccaa ccaatcaaac caaaagcaat cccagcaaca
    15361 aaaactttga agaacagcaa catactccat tcaggaatca cacccatttg gtaatgtaga
    15421 tgagaaacac catagctttc tgtcatgaaa tttgcaaaaa gcgcagcaaa gaagctagga
    15481 aatattgctt ctgttcggac tttaccgatt gccaacactt ctaatccaaa taatgttcca
    15541 gctaaaggtg tgccaaatac agaactaaaa cccgcactga ttccactgat aaccaaaatc
    15601 tcccgttctg ctttatccaa tcggaataaa tgcgctatat tatcagctaa tgctcccccc
    15661 atttgaactg ctgttccttc acgaccaaca gatcccccaa ataaatgggt agtgatggtc
    15721 ccaaacaagg tcaatggaat gagccgcaaa gggatcttct cttctcctcc attcccttga
    15781 tcgatcacta agttattccc acgactagaa agaccgccat gatacgcata aagataagca
    15841 aaaagcgcgc cgctgactgg caatagataa agtagccaag gatgtgctag tcggatatct
    15901 gtcacgaacg tcaggctatt taagaaaaaa gcagacaagc tccccatgaa tactccgaga
    15961 atcgctgtga ccaccagcca cttcaacatg tacatcaaaa tacgcactgg actcggtaat
    16021 tgtttttcca taaaaaattc ctcctcaaat aaaaaaacgc ctactaaaga aaggggctgg
    16081 ccccagactt tagtaggcgt cattagctat cttacgttag catttaaagg cgaacaccat
    16141 cacctattca cttccattaa aagatagcat atttttctgt aaaaaacaat aaaattatcg
    16201 ttatttcttt acacgttcag caaattcata gaaaggagcc aatttcgtta actgttgatt
    16261 acattgatca ctatcatgac aaatataatt cccctttttc gtatacgtgc catcagaacc
    16321 ggctttcgtt gtagaaagaa agagcgagac attcgatgtt ttctgacaga tcgcacagat
    16381 tcccttatta atcgttggtg ataaagtacc gctgatccct tttaattttc catcttcgta
    16441 ataaaccatg aatttttttt gcgtccctgg atcattccaa ccgatgtacg tatgtgcttg
    16501 caagtcgatt tcattccaag cgggttgttt gagttttttc acttttcgga ataatttctc
    16561 gatttgtggt ttgctcggct gcataaacgg ctggacgatt tgcttcagct cctccaatgc
    16621 tcgttctgct tgcgtttttg tcagttttac atctaaaact gtcgttaaaa acgtatcgat
    16681 ttctgatggt tcgtaaccta atacatcgcg aatcttttca gctgctagag cctgaaccgt
    16741 ttgtaccgtt tttgagtcat tgacagatga gtacgcattt aataactgct ctacttctct
    16801 ttttataaaa aaatattgat atggtttcat tgtttgttcc atgattttaa agtcctttct
    16861 tctaaccaaa gtgcatactt tgtagaaaaa agtgatcttc tgcaaagtat gtgttacctg
    16921 aagatcccgg actcttttaa ataagatacg ttttgcatct tatcatcacc ccctttaatg
    16981 ttcattcttc tatgtacatg attcattcga taaataaaag taaaccgtat cttgagcaga
    17041 tagtcaactg caaacagaaa aaggactgtg acaaaagtcg tgtcaaagtc cctttctaca
    17101 aacaaacggt gttccagaag tagcttcttc aaaaaataag ccggaatttc acaaaaattt
    17161 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgacccactt ctgtcacaac
    17221 ctcaatatgg tctattattg gttttacctg ttaaatcgag gattcttttc cgaagtaacg
    17281 ttgccatctc ttacgcatac ttgctgacat aggctgagcg gcttctttga ctgcgcagta
    17341 tttgtctacg aatgttacga tatatccttc tttgtactta ggcggtgcaa tcgttgcgcc
    17401 ccacatgtgc ttcaaaatga tatctcgctc aagatccgac aaatcggtga tcttttctgc
    17461 attctttaca gcgattcttg gatggatata cgcatgtgtg ccttcatcaa atttcgtcgt
    17521 acgccaatca tagtagaaca gatcatgcaa aagtccagca cgcgctgttg ctttagcatt
    17581 gccaccccat ttttttgcga gcagataact atagtaagaa acactgatgg aatgctccaa
    17641 acgagtggaa tgaacatgct gtgtgtaatt cgctaatttt ttcactgctt ctgtttctaa
    17701 aagatcttct acataagaca tatattcttc gtcttcacgc catttttctg tcataattat
    17761 cgatctgcca cctttttgaa tttcattttc ttctgaaatt gatctaagta taccaccagt
    17821 gttcggatta ttctagtcgc cttggataaa atagccaaag cttaatagtt ccttaatcat
    17881 aaagaaaaat gataaagaac aatccctgcc gcaaccccaa cattcaacga ttccgcattt
    17941 cctttcatcg ggatatatac gttctgatca gtcaaagaca agatttcctg acttaccccc
    18001 tgaccttcgt tccccatgat tattgcgaag ttatctgatt tttctacttt gtctaaggcg
    18061 tgtgcttctt catttaattc tgtgccatac actgtaaacc ctttgtcttt cattaaagga
    18121 atcacttctg tcaaatcttt ttgcaacaca ggcaaatgat aattgcttcc ctgcatgctg
    18181 cgcagcacct ttgtactata aatatctgcc gttcctttgc ctaataaaac accagccata
    18241 tcaaatgcat ctgccgtacg gatcatcgtc cctacatttc caggatcttg tacattatct
    18301 aataacagcc atcctcctga aaaatctggc gtgccttcag ccgttaacgc catcactgct
    18361 aagattcctt gtggtgtagg tagatccgat acagcatcca tgacctcatc cgaaactaga
    18421 aaagtagaaa tctctttgtt tgcggccagc cagtctcccc attctttcat gcctcgctgt
    18481 gtcacaaaaa gttcttttat ttctgcatca gacgacgcag cttcttcaat cagatggaac
    18541 ccttccaaaa gatattcttc cttttcttcc cgatatttct ttttatgtag tttttttaat
    18601 tctttgattc ttggattttt tgctgattgg atctctttca cattcctcac ctccccatga
    18661 ttatatcatg gaataggtgc ttgagcttgt ttcatcctaa aaaaatcggt atcattaatt
    18721 tataaaatag gaaagaaagg tgacgaacat gcgcaaagtt aaaatgaacg tccaaggacg
    18781 cgtccaaggc gtaggatttc gctacatgac caagatggtc gctgatcaac taggcgtgac
    18841 tggaacggta aaaaacgaag atgacggctc agtcacgatc gaagcaatcg gcaatgacga
    18901 catcatccag aaatttattg aagaagtcaa gaaatctccg agcccaagtg gacgagtgca
    18961 gtatgtggat gtccaagaag acccgatgat tgaggaacga aagaaatttg atgtggtggg
    19021 ataaggttgt gaaaaagctg gtctgcatca agcattaagg acaggttgtg aaaaatagct
    19081 ctccatattt ttcaacaggc tggacttaat gccgtagatg caagcttttg aacaccgtca
    19141 ataaggttgt gagaaagtcg cttagttttg agtagtaagg agaattttgt gaaaatggct
    19201 ttccacattt ttgcataaaa tttgacttaa taccgagaaa ctgacttttg aacaccgtcg
    19261 ataaggttgt ttttgagacg ctttgcctca agtattaaag ataggtagta aaaaagctga
    19321 tctataactt gcaataaatt tgcttaaccc atattttcca gacagaacaa aaaagttttt
    19381 agttttttaa ataagaaaga aacacttgga atcactcttt tctttccaag tatttctttc
    19441 ttgatttttt gtcttttttt caaaattttt cgctacgtaa aagaacgaat atttatatga
    19501 aatttctatg aagttgattg aattccatgc gcttttttag tatagttaag tttgtgtaaa
    19561 aattatagaa gaggaagtat tctatgaata aatggaaaaa ctggttattg ggatccggac
    19621 ttgtcagtat cctgctcttt ttatctggct gtgtgcggat ggattcaaac ggaaaccctg
    19681 acacttcggg aatcgtttat cgagtactcg tccatcctat gggccaagcg attacatatc
    19741 tagttgagaa tttcaattgg tcttatggct gggcagttat tgcaatgact gtgatcgtcc
    19801 gtatcatcat cttgccacta ggtattagtc aatcgaaaaa aacaatgatc caatctgaaa
    19861 aaatgcaagc gttaaaacca caagttgaag cagcccaaca aaaattgaaa gctgcaacaa
    19921 cacgtgaaga acaaatggca gcacaagcag aaatgcagca agtataccgc gaaaacggat
    19981 tgagtatgac cggcggaatc ggctgtctgc ctctattgat ccaaatgccg atcttctctg
    20041 ccttgtactt tacggcacgt tatacagaag gtattcggga atcatctttc tacggcatcg
    20101 atttaggctc accaagtatg gtacttgttg tcattgcagg ggtcgcctat ctgcttcaag
    20161 gatacatctc aacgatcggt attcctgaag aacagaaaaa gacgatgcgt acgatgctaa
    20221 tcgtctctcc tgctatgatc gtcttcatgt cgatcagtgc gccagccggt gttacacttt
    20281 actgggtagt cggtggtgtg ttcagctgtc tgcaaacatt catcacaaac gttatgatga
    20341 aaccacgttt gaaagcacaa gtggcagaag agttgaaaca aaacccgcca aaacaagtcg
    20401 tcactccaag aaaagacgtg acagaatcag caaagccagc taacaacaaa caagcaacaa
    20461 aacaaatcaa acaaccaaaa aacagtacaa acaacaaagg ccgcaacgca ggaaagcagc
    20521 agaagcggaa ataaaaacat aggacaaaaa agatgagagc taagccctca tcttttttgt
    20581 tactcttcct cttctgctat tccattaatt aattcgttga cagagacaat gctttcacat
    20641 cctgcacgaa caaactcggg ctctgaacca atcatttgtg agttgataaa ttcaatgacc
    20701 atcggaagat tcatcccagc ataaagagta atctgttgtc cttccattat tagtttagaa
    20761 atgatatttg caggtgtacc acctaaaaga tcgcatagta ccacaaaatc atttaacgaa
    20821 tctgtgacag tgagaaactt ttgtttgtat gtttcagtgc catcttctgg taataaaggt
    20881 aacgtgtaga tatcctcttg aggccccatg atcatttctg tacttttttt caattcctca
    20941 caaaattttc cgtgactaat caataccaat tgtctactca tttttcgctc tcccttattt
    21001 cgaaatcaca cccaatgcag ataaagcaac acacacaatt aatacgataa aaatcgctcg
    21061 agtaggagtc atgtttttct ttcctaataa ccaataaaca ccaccgacga tcccagcagg
    21121 taaaatttta ggtaagatca tatcgatata gttttgcata ttcaatgtca catcgccaat
    21181 cgttggtgcc catgaaaatt taacattgat cattgtcgca actaatgccc ctaccatgaa
    21241 tacccctaat aaagtggctg cgtccgttaa tgcagttaag cgagattgca tagtagtaac
    21301 taaggaaatt ccttctttgt atgcaaattc aagttgcttc caacgaaaaa ccatgacaac
    21361 gatctgagca aaaatccata taaacacacc tatcggattt ccatcagttg ccatattcgc
    21421 cgccaaagcc ccaaaaatcg ttggaatcaa agcaacaaat acggaatctc caatcgctgc
    21481 aaaggaaccc atcaatccag ctttcagccc cgatacggtt tcctttgaag caattccctc
    21541 tttttcttcc attgcaagat caatcccagt aatgattgta ttaaaaaaat tagaagtatt
    21601 aaaaaattgc gtatgtgttt tcatcatctc ttttaattct ggtgtattgt ctccatatat
    21661 ttttcttagt tgtggcaaga tagtatataa atagccagaa ccctgcattc tctcatagtt
    21721 ccaacctaat tgaaaagtga acaaacttct tcgattgatt tgcttaaaat cttcttttgt
    21781 taatttgtaa ttagatttcg tcatcttcga tttccccctc aacagaatgt gatggctcac
    21841 tagctaatgg ttgatggtct ttgttttgat cattcttata actgatcatt gctagtgcta
    21901 gaccaatcaa tgctacagca agcatcggca gcgaattgaa agttgccgga agatctttaa
    21961 caacgcttgc cacacttgtt cctaataatt gaacattaga gaataacgtt gctaataatg
    22021 ccgtgatcgt aaatcctaaa ataagataag atagatgttt ttttactggt aaatagcgca
    22081 gaagaatggc aaacccaaca gctggtaaaa cagctcctgc tactgaaagt ccatcgccta
    22141 gccattttag attcccgttt aaaacagaaa caacattttc aactaatcca ccaccaaatg
    22201 ctaaagctaa aaatacagga atcatacgag agagcgacca aggtaaagct cccattaaga
    22261 aattgcgttc gattgcttga taattcatgt cttctacgtg acggtcaata cgatgggcaa
    22321 agtatgtgtt agcgaatcta gctaaaatat ccaattggat cattaagctt gctacaggca
    22381 ctgcaatagc agcaatcgct tgttctggat tcattccaag agaaatcgag aaggcagttg
    22441 ctaaaatcgt cccagagttg gcatctattt ttgatgcgcc tccaaacgtc cccactccta
    22501 aaactgtcaa ctgcatactg ccaccaataa tcaaacctgc gtttaaatca cccattacta
    22561 aacctgcata taaccctgca aacactggcg cactcattga agaatagatc tgtaattcat
    22621 ctaaaatttg ataacctgca tataatgtta ataataagat ctgccaccat tgaatatcca
    22681 tggatcattc ctcctatttg attaaaagat tcataaagtc ttctgcttta tcgtttggta
    22741 ccatttgagc aattaaatgt ataccaagct gatctaattt tttaaatact tcgatatctg
    22801 catctaccac attgattgat tttgtaatgg accgtgtttc attcgtttgt gacatattcc
    22861 ccacatttac ttctttgata ggaactcctt tttctactaa agataagatt cgatctggct
    22921 tttttgctac gatcaatact cgttgagagt catatttgcc agctaaaata tttgttgctg
    22981 ctttttcgat tggcaaaatg cttaatttta cccctgctgg agtcgctaac ttcaatccac
    23041 ttttttcaat tgtgtttgtg gctacctcat catccacgac catgattcga ctaatattca
    23101 gtttcgttgt ccaaagattt gctacttgtc catgtatcaa acgtccatcg attcttacac
    23161 caataattgc catcatgcat tctccttatc tattttttct atttattgtt caattaaaac
    23221 tgtgacatca tcaaatatcc cttctgtttc aaagataacg ttttcatttt ttctttctta
    23281 tatttcattt ttaagctaag aacaaattcc ttcccaacta aagaaaacgc tttcttttat
    23341 atttaagttc atttgatttt cttaacttga atgtgtttca tcaacatcgt aactttcata
    23401 gggtcgatcc agccagtttt ctcttgttgc gcgtttgcca taccacaagc catagaaaat
    23461 gcgagaatct cttcgattga ttgccttttt gataacgcat gggccgatcc tgcaagggtc
    23521 gcgtcacctg aaccaacagg attaacaact gatattttag gaataatgac ttcgtaaaaa
    23581 cactgagcaa cttttgccac agcacctctc tctcctaaag aaacaacaac ccatggcact
    23641 ccttcaaata ttggatgagc taataaggac tgaatttttt ctaattgaat cgaagaacag
    23701 atacgttggc caatcagttc tcctaattct tccagattgg gcttgataag tagtggcttc
    23761 tccttacctt ccaatacttc aagcaaaaat ttaccagaag tatctagtaa tatgtctttc
    23821 ccttttttat cagctaaagc aaccaattgc ttatagtaat caagagggca tccttggggt
    23881 aaactacctg agatcgtcat aatatctgat atttcgagga gctgctcgaa atgatcggta
    23941 aatgtctgtt gttctttctt tgttatgaag gggcctcgtt ccaaaagctc tgtttgtctc
    24001 gaactatgca ggatagcaat agaatccctt gtctctcctt taattggaac aaactgatgc
    24061 atgatcccta actgatctag ctggttcttt agatattcac cgtgctggcc acctattaaa
    24121 ccagtagcta tcacctcttc tcctaataaa tttagaaccc tcgccacatt cactcctttt
    24181 cctcccgctg ttttattgac tgtttcaact cgattgactt tgtcgaggtt taattcttca
    24241 attgtatacg cgatatcgat ggaaggattc aaggtaactg tcgtaatcat ttcatcttca
    24301 cctttttgct ttttgtttta ttgaaccaag gggtagcggt ttcatttatt actttattta
    24361 aagtctcgat gttttctctc ccatttgttc ttaaccaatg aatagctccg tcttttccat
    24421 tttcgacgta tgctttgacc ccatctttcc atgtcgcacg tccacacaat acaccattga
    24481 aggtggaacc cgattcatga gcaaaaatca gtgtttgttg aaataattct gttgaaacac
    24541 ccccactcaa aaaaatgaat ggtaatggag tcgcttcact ttgctcctta aagaaggcag
    24601 cagcctcttc tttgctgtga ataaattcac ctgtggaata gccattcaca taattcatat
    24661 tgactggtac ttctactttc aacacatcaa ctttatattt tggatcagaa aattctttca
    24721 tcatctcaat aactttgtgg ggcttgactt tcgcatactc tggagaagaa ctatcgctta
    24781 tttgtgcatc ataagaaact aactccaaat agaagggacg attttctcct tcgcactcac
    24841 tacctaaacg ttcgatgaac acatgcttca aatgattgat ttcgggtgct tcatcaacat
    24901 cgtagtaaag taagaatttt actgcatccg cttgctgttc tttcaatctg agagaagacc
    24961 aatccgctaa taaatcaggt agacgaccag gcgtagttgc gtcatagccg gttttctcat
    25021 aagctaacaa taaccccgtc tcttttgctc tgacttttgc tgctggcaat ccatattccg
    25081 gatccaataa aatagcagaa gcatagggtg tcagctcagc tgatactagt tctttgaatt
    25141 cactgatttg gtcttcactt ggctcttttc ccaacgattt gatcattttt tttagagcac
    25201 cacgctgatc gatcgctaat gcactaataa tcccatcttt tgttgatagt ctctccattg
    25261 ccctattttt ttcttcattc atctgtcatt tctccttcgg tttcatactt atgaataatg
    25321 acgcccttga ctactcgatt gaccgtgcct gttggagatg gtgtatctgg tttattagct
    25381 actttgattg aagtatagag tgcaatgatt tgtgcagtca taatcatcgc taatgcaaga
    25441 taggcatcgg gcaatttact caccgagtct tccaatagca cagtcatacc tgagaaatca
    25501 atagtacccg gttgcgcaat cgcaatgaca ttggcagtga cccgatcttc ttttatttcc
    25561 tctaaaatat ctaagtcata atttcgcgta tatggttgat tgctcacaaa acctacaacg
    25621 agtgtctcgc tatttaaaaa agactttggt ccatgacgaa accccatcga agaatcaaaa
    25681 attgtagcaa ctttacctgc tgttaattct aaaattttta attgtgcttc tcttgtcgca
    25741 cctgccaaac ttcctgaacc cagataagcg atccgttcaa aaaaatcagg taataacttt
    25801 tgaatttctt gatctcttga aagaagatct tgaccgattt taactaattg ttcaagatat
    25861 ctttcttttt tctccattgc tgtttgatca aagatccata aagcactcaa taacatacac
    25921 gtaaaacttc ctgtcatagc aaaacctgca tcgtttgctc tctctggcat caataacaga
    25981 taattctgtt tatcatcctg tgcgtttttt gccaacatcc cctctttagc acaagtaatc
    26041 gtcaaatgaa acaatgtttc aacttgttca tttgcaattt gcacagccgc tacactttct
    26101 ggactgttgc cactgcgagc aaatgaaact aataaagtcg gttcatttgg aaacaaaaat
    26161 tcttttggtg aagctacaat atctgtcgtg ccaaaactgt ggaagataaa aaactgattg
    26221 tctccatgtt catttagata gggtaagagc gtatccccaa tatattgaga cgttcctgca
    26281 cccgtaaaaa taacacgtat tcttcttccg tttgcttctt tttttatttg atcaaaaaat
    26341 agagctaaat tttcttggct ttgcatcaat ctatcgattg tttctttcca gagatctggt
    26401 tgttgtttta tttcttgtgt cgtgattgct gcacctattt gttttaattt gttcttcgat
    26461 agctcaaaca tgttgttctc cctctcaatc attttcggat atgccgaatt ttataatgaa
    26521 actggtccgc tcttgctaca ctatctgtat attcgataat ctcatttttc acgttatagg
    26581 ttatcctcgt cagatgtaag acgggagcgc catcagcgat tcgtagcaca acagcatctt
    26641 ttttttgcac gatacttgca taaaactctt catcagctaa ttgaacttgt tgttcataat
    26701 cctgtgaaaa cacatcatac aacggtttac tttcaagtaa cggcaaatca agtcccataa
    26761 atagtttcgc cggtagataa gaacgctcaa ccatcaatgg ctcttcatct gccaaacgca
    26821 atcttttaag tttaaataca gtttcaccta aatggatgtt caaattctta gcaattctct
    26881 tattagcttc catttgtcta aattctaaaa tgaccgtctt agggacgcgc cctatttcgc
    26941 gcatttcttc tgtaaagcta tatgctccag ctaagttcga tgacttatca gacaaatcag
    27001 agacataggt accttttcca tgtcttcggt aaatataacc ttgattttcc aattcctgta
    27061 aagccagacg tacggtggta cgactcaccc catagtgttg acaaagttct ctttcagata
    27121 acagcttatc atgcggagtc atctgtgttt cgatacgatc cttcaataaa tcaactagtt
    27181 gataatacaa tgattttcct ttatttgagc tctccataaa aatcacctct tctttactgg
    27241 ttataaccac aacacaagaa tagtgaatta ttaatcattt gtcaaaggtt ttttagacca
    27301 aaaaagctat tataacagct ctttaaagct ttatatcttg aatagacaaa gatacctcaa
    27361 aaataccagt taccaataaa agaacagaaa aaaagacgaa ccacataagc gatccgtcaa
    27421 aatggtctaa cttatcgagc agcgtgatcc acattaatcc ctatgatatt cctacaacta
    27481 tcgttttatt acttccaata tcatttacaa caactaaaaa tagcttatta atgacaaatg
    27541 ttattattag ttttagataa cttttgcagt tttatacttt ttttctataa aatttgacaa
    27601 acattttttt aaataactaa ttattttttg aatctgtcac tcagacaatc ctttctctta
    27661 cttgttaaca taaatagaag ttgctgtttc ttctaatagt gtacttaatt ctttaatatt
    27721 ttgttttcct tcttctaaca tccaactact cgctttttct tcgccttcgg taacaaaagg
    27781 tgctactccc ttcgcccatg ttgctcgccc acataagaca ccattgaatg tcgaacctga
    27841 ttgttttgca aaacgcaatg tatcttgaaa tagtgcagcg ctgactcccg cacttaaaaa
    27901 aataaacggt tgttcagtca ctttcgattg ttcatagaaa tacgcggatg cctctgcttg
    27961 actataacaa gtatcttgcc catatccttc cacaaatctc atattcactg gtacttctac
    28021 tttcaataca tccacttgat aacgttcttt tgaaaattct cgcatggctt caatcacttt
    28081 atgcggtttt acttttgcat agtcaagaga agacggatcg ccaatagttg catcgtaaga
    28141 aaggagctct aaaaagaaag gtatgccttc tgcttcgcat tctgaaccaa tccgttctat
    28201 aactatttgt ttttgcacat taatctctac tggttcatca acgtcatagt acaataaaaa
    28261 tttacaagca tccgcaccca cttcttttaa ccttttcact gaccaactat tcagtaaatc
    28321 tggtagtcgg cctggcattt tcgcatcata gccggttttt tcataagcta gcaacaaacc
    28381 acattctttc tctcgaagcg ctgctgcagg cagaccatat tctggatcga gtaaaatagc
    28441 ggaagcatac ggagtcaggt actgagaaac catctgttta aattggatga tatcttctga
    28501 tgtagcttct ttatattggt tgatcatttt cttcaaagca ccacgttggt cgatagctag
    28561 cgcgctaata attccagctt catccgtcaa tcgcttcaaa cgttcctgtt tttctatact
    28621 cattttcttc atatatcttc acctatttct ttagttaata ttgtagctat ttctattttt
    28681 gattttgcag ctctcaactg ttctgtaacc tcttttcgca tcaatgatcg tgcaacgaga
    28741 gacaataatt tcaaatgagt cgttcctgct tctgaatctg ggataaataa cgcaaaaata
    28801 aaatcaactg actgtctatc taaactgttc cattcaattc cttttttcag tgtgacgata
    28861 atcaaactag gttcattgat tgtttgagac ttcgcatgag ggatagcaaa acctgacatt
    28921 attcctgtag taccttcttt ttctctttcc agaagttttt gatatacttc tttttcgtca
    28981 tgagcgattc ctaaacagaa tgtttgcttt gctaaaaact taaatgcttc ttcttgatta
    29041 tctaatggct gttgtaaaaa aatgttctct tcatgaatga ttgtcatgat aaacctcctg
    29101 ctcttccttc ttcgataact gtacgatttg ttgctttatt atccctcttc ttgtctttat
    29161 cagttactct tttcttttgg atcaaaccta gtatcactcc accaacgatc gaaccaataa
    29221 gaatcgctgt aacccattgg atttttcccg tcactacagg taacactaaa aaaccgccat
    29281 gtggggctgg tacttggaca ccaaacagat aggtcaagac agcagcaatt gatgagccta
    29341 acatcatcgt tggtaatact cggatcgggt ctttcgcagc aaacggaata gctccttccg
    29401 taatatgagt tgatcctaaa ataaaattaa ccaaacctgc attacgatca ttagtatcaa
    29461 aatatttacc aaacaataaa gtagcaaaac cagtaatcaa cggtggtgca atgcaagctg
    29521 ctgacgcacc agccataaaa gtcgtgtttc cttgtgtcaa aagagctgtt cctgttacat
    29581 atgccgcctt attaaccggt cctcccatat caaacgcaca catacagcca acaatcacgc
    29641 caagaacaag tggattcgaa tattcaaatc cagctaaaaa gctcatcatt ccttcattta
    29701 tagccttcat tggacctgcc agcaaagaca tcacgatgcc aataaatgct acaccaataa
    29761 ctggatataa gacgattgcc ttcaagccat ctagttgttt aggtaaaatt atcagtagct
    29821 tttgcaaaaa taagataaaa taacctgcta agaacccagc taaaatacct cctaaaaatc
    29881 cagtaccgcc attcatggct acgattccac caacaaatcc aacgactaac cctggacgtt
    29941 tcgcgatccc ttctgcaata taagctgcta aaactggaac catcattccc attgctgcat
    30001 cgtcaactgc ttttaccatt gccgctgtag cattgtattg tgcatgatct ggatcagaag
    30061 aatagattcc ccaaacggca aatgaaatag caatcaatac tccgccagct accacgaaag
    30121 gcaacatatg agagacaccg ttcatcaaat ttttatagat gctacgacca aaattagcac
    30181 cacttatcgc ttctggttct aaagtatcag cagcttcatt atctgctaag acagtacctt
    30241 ttcctgccaa tgcttcgtca atcagccgtt gtgcatcctt gattcctgta ctgacagaaa
    30301 catcaatgac tcgttttccc acaaatcttt ctgaatggac atctttgtct gcggcaataa
    30361 tgacagcatc tgcctgtttg atttcttcag ccgttaattc attctcgatc ccaatttgtc
    30421 catgggtctc cactttgatc gtgatgcctt ttttcctagc tgcttgctct aatgcctctt
    30481 gcgccatgta ggtgtgcgca attcctgtcg gacaaccggt tgctgcaatt agttgatatt
    30541 ttcccatctt tcgaacctct cttccttgat tttgatttgt ttacttaatt cctccacatc
    30601 caaaaaatca gtcagtcctt tacgaaaagc agtagagctg cctgctgcta tgcttttacg
    30661 taacgtttca tcaagcgatc gtcgattcat gtatcctgct aaaaaagtgc ctaacatggc
    30721 atcacctgca catgctgtat tgacaacttt tccttttggc gagtttcctc gaatcaccct
    30781 gtctttcgta aaaagaatcg ctcctttctc accgagggat aacaaaacat tttctgcgcc
    30841 tgactctact agtcgttgtc cataaacaaa gtaatcttca tctgtaacca tttttcgtcc
    30901 gaaccaagaa gaaagttctt cctcatttgg tttcagcaaa aacggtcgat agggtaaaca
    30961 atccatgact tcttgatcac tactatcaat aatcaaacaa atccgactga tttctattaa
    31021 aacctttgga gacagccctc ttggtaaact cccagaaaca caaagataat cccctgcttg
    31081 taatgctctt atttgattta aaaaattatg aacagctgtc tgcggtattt caggtccttg
    31141 attcactaat ttgtattctt ctcccgtttg attgacttgt gtaaatacat tgatacgcgt
    31201 cataccaggc acttcaataa actctgtttg gatttgtttc tgctgtaaat agtcttctat
    31261 atactttcct gtgaacccag cagaaaaacc taatgccgta cttgggaaat ctagcatctt
    31321 taatactagt gacacattca ccccttttcc gtttgcctga atgtcatcct cttttgtgcg
    31381 gtttaccata aaaggatgca tctcatctgt ttcaataaat agatcaattg ccaaattcat
    31441 cgtgcatgtg tagatagaca taaccaacga cctccttgat tctattatga aatacgcgaa
    31501 atagaaaatg ttttcttaat cgtcccaaca caaagaaaac ctttacattt ataaactcgt
    31561 cgtgcttttt ttaactaact tttggtgaaa gaaggaaaga atatgaatca caaattaagt
    31621 gaaacagagc agtacctctg gaactttatc gaacacctca tcctagagat ccctaactat
    31681 tccatcgtga aactaagtga acaagcaaat gtctcaacag ccaccattgt gcggacaatg
    31741 aaaaagaaag gctatgaagg gtttacttcg tttaagcatc atctaaaaga aggagaaaac
    31801 aagaacatca atttttcatt tttagataaa gtagacaaag gaatccgccg agccattcta
    31861 aaaaatgaac aagaagtggt acgtacaatc aatatgttgg aaatcgggaa cattgaagat
    31921 gcaatacaaa gaatcaaatt tgctgaccgg gtttttgtct ttgcccgagg attttcagaa
    31981 atgattggcc aagaaatgct ggtaaaactt caattaacgg gaaaaaattg tcagatgcat
    32041 actgatccag aaattatcaa aagcatcagt cggaaactaa ctaagaaaga cagcgttatt
    32101 tttgtttctt tgaacggtga gaaaacaaat aaaaacatca aaaataagat ttatgtcttt
    32161 atttttcaat tttttaa
//
LOCUS       DAJSAW010000028        31245 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJSAW010000028 DAJSAW010000000
VERSION     DAJSAW010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.028"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            202..1077
                     /locus_tag="ONT64_001488"
     CDS             202..1077
                     /locus_tag="ONT64_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302912.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="ONT64_001489"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="ONT64_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="ONT64_001490"
     CDS             complement(1642..2973)
                     /locus_tag="ONT64_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2302913.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="ONT64_001491"
     CDS             complement(3122..3835)
                     /locus_tag="ONT64_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2302914.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="ONT64_001492"
     CDS             4010..5434
                     /locus_tag="ONT64_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2302915.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="ONT64_001493"
     CDS             5733..6440
                     /locus_tag="ONT64_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCR2302916.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="ONT64_001494"
     CDS             6427..6750
                     /locus_tag="ONT64_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCR2302917.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="ONT64_001495"
     CDS             complement(6839..7876)
                     /locus_tag="ONT64_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR2302918.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="ONT64_001496"
     CDS             complement(8197..9180)
                     /locus_tag="ONT64_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCR2302919.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="ONT64_001497"
     CDS             complement(9595..10365)
                     /locus_tag="ONT64_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2302920.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="ONT64_001498"
     CDS             complement(10358..11293)
                     /locus_tag="ONT64_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2302921.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="ONT64_001499"
     CDS             complement(11609..13258)
                     /locus_tag="ONT64_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR2302922.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="ONT64_001500"
     CDS             complement(13375..15978)
                     /locus_tag="ONT64_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR2302923.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="ONT64_001501"
     CDS             complement(15990..17039)
                     /locus_tag="ONT64_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR2302924.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="ONT64_001502"
     CDS             complement(17065..17379)
                     /locus_tag="ONT64_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HCR2302925.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="ONT64_001503"
     CDS             complement(17395..17841)
                     /locus_tag="ONT64_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2302926.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19764)
                     /locus_tag="ONT64_001504"
     CDS             complement(17842..19764)
                     /locus_tag="ONT64_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCR2302927.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(19976..20872)
                     /locus_tag="ONT64_001505"
     CDS             complement(19976..20872)
                     /locus_tag="ONT64_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2302928.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20865..21545)
                     /locus_tag="ONT64_001506"
     CDS             complement(20865..21545)
                     /locus_tag="ONT64_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2302929.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21546..22328)
                     /locus_tag="ONT64_001507"
     CDS             complement(21546..22328)
                     /locus_tag="ONT64_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2302930.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22321..23238)
                     /locus_tag="ONT64_001508"
     CDS             complement(22321..23238)
                     /locus_tag="ONT64_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2302931.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23402..23725)
                     /locus_tag="ONT64_001509"
     CDS             complement(23402..23725)
                     /locus_tag="ONT64_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302932.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            24513..27740
                     /gene="fss3"
                     /locus_tag="ONT64_001510"
     CDS             24513..27740
                     /gene="fss3"
                     /locus_tag="ONT64_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HCR2302933.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27853..28167
                     /locus_tag="ONT64_001511"
     CDS             27853..28167
                     /locus_tag="ONT64_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2302934.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            28180..28554
                     /locus_tag="ONT64_001512"
     CDS             28180..28554
                     /locus_tag="ONT64_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2302935.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            28564..28899
                     /locus_tag="ONT64_001513"
     CDS             28564..28899
                     /locus_tag="ONT64_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HCR2302936.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            28982..30322
                     /locus_tag="ONT64_001514"
     CDS             28982..30322
                     /locus_tag="ONT64_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCR2302937.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            30400..31074
                     /locus_tag="ONT64_001515"
     CDS             30400..31074
                     /locus_tag="ONT64_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCR2302938.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            31067..31231
                     /locus_tag="ONT64_001516"
     CDS             31067..31231
                     /locus_tag="ONT64_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302939.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
BASE COUNT         9736 a         6069 c         5515 g         9925 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt tctaatattc
    19861 ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct tttttctcta
    19921 ttaaatatat ataagataat acatttttct actagttctc actaatcttg tataattaaa
    19981 ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg gtcatctctc
    20041 ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta gattccgtat
    20101 ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca gaggtttgcg
    20161 ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct tttgaaaatg
    20221 tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt tgcagcactc
    20281 gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca gcgaacgcta
    20341 cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct tccacctctt
    20401 tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt cgagcaagat
    20461 cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat
    20521 acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa ccgttaatag
    20581 cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg atgttttctt
    20641 ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta ttcagtgcat
    20701 ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca ccatattctt
    20761 gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt tgcaggaaga
    20821 aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc aaatctaaac
    20881 cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt ccctcgaaga
    20941 ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg ccaaactgtt
    21001 tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga
    21061 tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc cctcgactct
    21121 ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac ttggacagaa
    21181 gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc aaatggtttc
    21241 gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg atcgcttttt
    21301 gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa caatgcttca
    21361 cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata tgtgcttaac
    21421 aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg ttcaagaacc
    21481 tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt ttccagcagg
    21541 accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga tgataccacg
    21601 attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc ttgttgcgca
    21661 aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc caagcctttg
    21721 atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa agaaagaagc
    21781 aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga taatgtccct
    21841 aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata gtttttgatt
    21901 ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc
    21961 aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac ataataagtc
    22021 attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa ttcttcccca
    22081 atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat tttttctacc
    22141 attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa ttcattgccg
    22201 tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt
    22261 cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc agttaataga
    22321 ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt tttgcccaac
    22381 taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga tggctgctac
    22441 ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat aatccctata
    22501 accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa
    22561 ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt gtccttcatg
    22621 caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc tagaaagcaa
    22681 gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt ctgccatccc
    22741 tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat tcgtgatcag
    22801 tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg ctttcttttt
    22861 ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat ccgacacacc
    22921 gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat aaagagcagg
    22981 ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa
    23041 ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa aggtagactt
    23101 acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt cgattgatac
    23161 tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt tcaagaccgt
    23221 ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt atagttgacg
    23281 ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa ttgaatgtca
    23341 aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt atacgcttgt
    23401 atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc tcttcactga
    23461 aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt ataaggataa
    23521 ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt tgcgttgtct
    23581 tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc atggtaatcg
    23641 ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc tgaagccaga
    23701 ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa aaataataca
    23761 tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag acttttcaca
    23821 atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    23881 ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag ctgaccactt
    23941 ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat ctttttcccg
    24001 tttgtttata gatcgaatga tattatagac caagaatcct acctattaac atagctaaaa
    24061 acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga ttaaggtaac
    24121 tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg aacgataatc
    24181 gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct ggttttttct
    24241 tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg taaatatatt
    24301 ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc
    24361 agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat ccattctatt
    24421 cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt
    24481 tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat
    24541 gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt gctcctacga
    24601 ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac
    24661 atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc
    24721 cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa ccaggagtcc
    24781 cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat atgtccgata
    24841 aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg
    24901 tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat
    24961 cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa gccattgacg
    25021 attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta gggcaatcga
    25081 ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg
    25141 ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca aatgccaatt
    25201 caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca cctgtcgcct
    25261 ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct aatacctaca
    25321 caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc gataaagaat
    25381 cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact ttacctgcaa
    25441 aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct catggtacaa
    25501 aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc acccccatga
    25561 ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag
    25621 ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg aatgacaatt
    25681 attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt gaaatcgttc
    25741 aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt gctaatgcgt
    25801 tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt
    25861 tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct gttaccagta
    25921 acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag
    25981 atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac accttgttta
    26041 ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag ttagcgaagg
    26101 gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac caagttgccg
    26161 ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc gaaggatata
    26221 gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg
    26281 ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc tttgatttct
    26341 ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat ctgaccttta
    26401 aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag
    26461 cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg
    26521 attatttact tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa
    26581 tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat gtctttacca
    26641 ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac gagaagctga
    26701 cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg cctgaggaag
    26761 aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta acttctctgg
    26821 attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa gaagactggt
    26881 atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa
    26941 aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag
    27001 gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa actattgtct
    27061 tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag ccagtagcga
    27121 aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa caccatgttt
    27181 ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat ggtgatgtgg
    27241 tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca aaagaagctt
    27301 tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt tggaaatctg
    27361 gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt gcgaaaaaag
    27421 tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc aattacgata
    27481 aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaaccttaa
    27541 caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca ccaactgttc
    27601 caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc ggggagaaaa
    27661 attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg ggctattatt
    27721 tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg
    27781 cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa
    27841 gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac ctttggaaag
    27901 ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa tactacggtg
    27961 attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat tgaggtggta
    28021 gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa agccgtgaat
    28081 ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga ttacgtgttg
    28141 tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt
    28201 gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg ccgagaagta
    28261 cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac ctataattta
    28321 aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt acccttacgc
    28381 gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac cgttgccaat
    28441 tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga tttagtcttg
    28501 aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag
    28561 attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggcgag
    28621 ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt tctgattata
    28681 catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt actagactgg
    28741 ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag
    28801 gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga aggcttttta
    28861 aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac tagcataact
    28921 ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg aaaccatcaa
    28981 aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc actttgttct
    29041 tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagtttag
    29101 acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt ctataaaaga
    29161 tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc tttatattca
    29221 cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc ggatgattct
    29281 agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa
    29341 tagtagccga accagagaga ctatcagtta cttccccaaa atctgttatc gaatgaaaga
    29401 tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc agcttttaaa
    29461 attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg aactcaaaga
    29521 ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg ggatagacga
    29581 agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg cttatgattc
    29641 tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt tcctcctcac
    29701 cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc ccaaaaatgc
    29761 agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc
    29821 tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat
    29881 gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca actttttaat
    29941 ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg cggtgatttt
    30001 gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc tgattttggc
    30061 ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatcaat tcaactttcg
    30121 cgtggctttg ggtcgtatga gtgaactcgg atattccatg atgtttgggg aggtcgaaaa
    30181 aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat
    30241 tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttagatt caattagtaa
    30301 attagaaaag atgaatgatt gagtgatctt caatactgac tttatacttc aagtaacgta
    30361 taatactttt atatagtagg aagaaagggg ataattaata tggaaagtaa tgattcttat
    30421 ttagagtatg caaaagcaaa taaggataag tttatttctt atattatcaa agggaaatcc
    30481 cccgaagttg gacgagacgc agtttttatg gcagggagtc ctggggctgg aaaatcagag
    30541 gttgccattg gattagctga gaattatgat aaccatgtga ttattgatgc ggattacttt
    30601 cgcacccagt ttcctgatta taatggaaaa aactctagtc tctttcaaaa agcatcttct
    30661 tggttggtag agcaggcatt tagatatgtc ttagatcatg gctattcttt tattctggat
    30721 gcaacttttg caatattaag tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt
    30781 cgagtgacga ttttctatgt ttatcaagac cctaaaatcg cttggaaatt tacacaagaa
    30841 cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa
    30901 tcaagagaga acattgcaaa agtaaagagt aaacatccag atgtattgct tcatatcata
    30961 ataaaagatt atacgaataa tatttccgaa gttcattatg ctacggataa tctagaatta
    31021 tttttaccac tagggtatag taggaaagat ttggaggttc aattacatga ctgagaaaga
    31081 acaagtaacg aaaattgtaa agaaatacaa taagagcatt gcggatttat cggaaaatgc
    31141 tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc gatcaggcca atgaaaaaca
    31201 acggaaatta gttggattaa ataaaaaata gtaggacaaa ttaat
//
LOCUS       DAJSAW010000029        30490 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJSAW010000029 DAJSAW010000000
VERSION     DAJSAW010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.029"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            137..475
                     /locus_tag="ONT64_001517"
     CDS             137..475
                     /locus_tag="ONT64_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302940.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(680..1255)
                     /locus_tag="ONT64_001518"
     CDS             complement(680..1255)
                     /locus_tag="ONT64_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCR2302941.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1987..2568)
                     /locus_tag="ONT64_001519"
     CDS             complement(1987..2568)
                     /locus_tag="ONT64_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2302942.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2751..3377)
                     /locus_tag="ONT64_001520"
     CDS             complement(2751..3377)
                     /locus_tag="ONT64_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR2302943.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3399..4730)
                     /locus_tag="ONT64_001521"
     CDS             complement(3399..4730)
                     /locus_tag="ONT64_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCR2302944.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4885..5286)
                     /locus_tag="ONT64_001522"
     CDS             complement(4885..5286)
                     /locus_tag="ONT64_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR2302945.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5372..5578)
                     /locus_tag="ONT64_001523"
     CDS             complement(5372..5578)
                     /locus_tag="ONT64_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302946.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5673..7175)
                     /locus_tag="ONT64_001524"
     CDS             complement(5673..7175)
                     /locus_tag="ONT64_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HCR2302947.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7193..7648)
                     /gene="argR"
                     /locus_tag="ONT64_001525"
     CDS             complement(7193..7648)
                     /gene="argR"
                     /locus_tag="ONT64_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCR2302948.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7746..8363)
                     /locus_tag="ONT64_001526"
     CDS             complement(7746..8363)
                     /locus_tag="ONT64_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR2302949.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8584..9957
                     /locus_tag="ONT64_001527"
     CDS             8584..9957
                     /locus_tag="ONT64_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2302950.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10032..11144
                     /locus_tag="ONT64_001528"
     CDS             10032..11144
                     /locus_tag="ONT64_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR2302951.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11216..12853)
                     /locus_tag="ONT64_001529"
     CDS             complement(11216..12853)
                     /locus_tag="ONT64_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR2302952.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13021..13629)
                     /locus_tag="ONT64_001530"
     CDS             complement(13021..13629)
                     /locus_tag="ONT64_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2302953.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(14013..15164)
                     /gene="queG"
                     /locus_tag="ONT64_001531"
     CDS             complement(14013..15164)
                     /gene="queG"
                     /locus_tag="ONT64_001531"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR2302954.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(15183..15935)
                     /locus_tag="ONT64_001532"
     CDS             complement(15183..15935)
                     /locus_tag="ONT64_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302955.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(16076..17026)
                     /locus_tag="ONT64_001533"
     CDS             complement(16076..17026)
                     /locus_tag="ONT64_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCR2302956.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(17027..17866)
                     /locus_tag="ONT64_001534"
     CDS             complement(17027..17866)
                     /locus_tag="ONT64_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR2302957.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(17863..18609)
                     /locus_tag="ONT64_001535"
     CDS             complement(17863..18609)
                     /locus_tag="ONT64_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR2302958.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            18847..20217
                     /locus_tag="ONT64_001536"
     CDS             18847..20217
                     /locus_tag="ONT64_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2302959.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(20272..21168)
                     /locus_tag="ONT64_001537"
     CDS             complement(20272..21168)
                     /locus_tag="ONT64_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2302960.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21282..21452)
                     /locus_tag="ONT64_001538"
     CDS             complement(21282..21452)
                     /locus_tag="ONT64_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302961.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(21449..22126)
                     /locus_tag="ONT64_001539"
     CDS             complement(21449..22126)
                     /locus_tag="ONT64_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR2302962.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(22217..23122)
                     /locus_tag="ONT64_001540"
     CDS             complement(22217..23122)
                     /locus_tag="ONT64_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR2302963.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            23297..24751
                     /locus_tag="ONT64_001541"
     CDS             23297..24751
                     /locus_tag="ONT64_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2302964.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            24767..25798
                     /locus_tag="ONT64_001542"
     CDS             24767..25798
                     /locus_tag="ONT64_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR2302965.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            25805..26992
                     /locus_tag="ONT64_001543"
     CDS             25805..26992
                     /locus_tag="ONT64_001543"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR2302966.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            27148..28530
                     /locus_tag="ONT64_001544"
     CDS             27148..28530
                     /locus_tag="ONT64_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR2302967.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            28502..28888
                     /locus_tag="ONT64_001545"
     CDS             28502..28888
                     /locus_tag="ONT64_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302968.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(29088..29756)
                     /locus_tag="ONT64_001546"
     CDS             complement(29088..29756)
                     /locus_tag="ONT64_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCR2302969.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(29945..>30490)
                     /locus_tag="ONT64_001547"
     CDS             complement(29945..>30490)
                     /locus_tag="ONT64_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR2302970.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
BASE COUNT         8551 a         6236 c         5572 g        10131 t
ORIGIN      
        1 taaaaaaata atccaagaaa cattgaacct atggatccaa aagctaccca aatactatag
       61 ctaacccccc gctaccttca attttgcaga taaaacgctt tgggagaaaa tttcaaagac
      121 aacaggccat cctatcatgc ccgattataa acaaaaagct ggctattcct tttgctctga
      181 caaatttcaa ttgggaaagt tcacgaaact agcaggtgaa gaagtttcaa ctgttcggat
      241 caaagaatgc ccgcttcaaa tagaaacatt ggttcaacga atttcaacta gacaagactt
      301 tgctattgta gaacgtgaga tcgtagctat ttttgtagaa gaagggattt tacatgatcc
      361 aactcatatc aatgtggata aatggcaacc tcttatctat aaattcagag aatatacaac
      421 tgccaaccag tcgttaactt tgaattttag attccaagaa ttcaaagagt cataatgata
      481 aacatcgtta ggtataaata aaaaagactc gcagcacgag taaaaaacaa atagaagcta
      541 tacattagaa aggtcgactg tgaccctata gatgcagtat atgcagctta tgagcatttc
      601 ttctgttctc tttgtttttg ggatctgtgc tgaagtcttt ttcgtttttt gccgctcttt
      661 ctttgaaaaa tcgattatgt tataccttct ccattactaa ttcaggcatc tgctgagtaa
      721 tgatttttcg cgcaaaagaa atatctgtta cccaaggttc gatttgttct tcagtcaaga
      781 caagcggcat tcggtcatgg attggtgcga ctgtgcggtt aggttcagta gtcattatga
      841 ttgattctgc cattttttca tcctgatggc tacgataaaa accgccaata tacactattt
      901 tttccttatt tgaaaacata aatttgtctt tttcttcatt ccactcataa aagccactca
      961 ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt tcttctactg
     1021 tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat ccccacttca
     1081 tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca agatttgacg
     1141 gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc tgggcttctc
     1201 gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca cacatcctaa
     1261 tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc aacaaaacgc
     1321 tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca gtgaatggta
     1381 ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgcttt
     1441 ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct gggtaaacgg
     1501 tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta gaaagccatt
     1561 ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct cttccttaaa
     1621 cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tatgagcagc
     1681 tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca acctctccac
     1741 taaacggtgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa aatatgagga
     1801 actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca caacctctaa
     1861 tacacggtgt tccagaagta gcttcttcga aaaataagcc gaaatttcac aaaaatttga
     1921 gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc
     1981 taattattaa agaacctttt gcgaagttaa caatctttct ttgatcaatt gaaaattctg
     2041 cctaagtagt gatagatcct gtgtgacttt caacaataag atacttccct ggatttcagc
     2101 aatcaacgct ttggcttgac ggtctgcttc ttcatcggac agtttttctt cagaaagtgc
     2161 caggatattt tgtttcgtat acttgaccca ttgattgact aattctagta gcaacagacg
     2221 gtattcttca tccactgtac tcaactcaat aatcaaatta cctacaggac agccatgata
     2281 agtgatctgt gtctcatgat cagcgaaaat ccagtccaac atttcttcta atgcttcttc
     2341 tggctgtttt ccagcattta agatgtcttc gaacaattct gtctgccata cgtccatatg
     2401 cgcatggatc accgctagac aaacttcttt ttttgaagaa aagtaataat aaaattgacc
     2461 ttttcctgta tcagaagctg ctagaatatc tcggattgac gtttcatgat agcctttttt
     2521 ataaataaga tctttggtcg cttccaagat cttttccttt gttttcaatg atattctcct
     2581 ttttcatttc tcatagtcct atattataca gtaaattcat cattttttct tcttaatcgt
     2641 ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac tataaaactg ttctcatata
     2701 gctagatgtt cattgattca agaactgcga ggacaaaagc aattcctatc ttatgatagt
     2761 tgctctaata cttcctctgt tgtcatcact tgatctgcaa taaagccaaa gttcgtcaac
     2821 gctgcatcca ttgcttcttg gccaggagca ccaattgcat ctgtcgctac cacaacattg
     2881 aatccactct ctaccaattc acgcatatgg gaatcgctac acaagtttcc gttcattcca
     2941 ccaagaatta ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc attgctttct
     3001 ggtccaaaga ttttatgagg agagctgatg atcgtatttt catccaaata aggtttcaat
     3061 tcttcgataa aatcggctcc tgaaccttct tccactgatt catattgact ttttctagca
     3121 aacatctgct gctcaagcat catatgttct cctgcaccgc caaattgcca tttatggtca
     3181 tgtgggtaaa aataatgagg agaaataaag agtttgtatc ctttttcttt tgcttgtttt
     3241 accatttcaa tcatattttc gatcgtattg tatttttcta agatatcttt tgtcaaacca
     3301 aagccaccgc ctgctggttt caagaactcg acttgcgggt cagtgagaac tactgctgta
     3361 tttttattat tgaacatggt ggattctcca ttcgttattt attttaatac atcatttaga
     3421 gcttcactca ttgtcggatg agaatagatt tgatctctca ataacgtata aggaagtttt
     3481 ccattcatag ctagcgaaat caagttgatg acttcatgag aatcttccgc atagatactt
     3541 gcccctagaa tcaagtcagt ttcagggtcg actaaaactt tgaatacacc tttaggatct
     3601 tctaatactt gtgctttagg gacaccgctt gtcatatact tgaataattt gtaattgacg
     3661 ccttgtgctt tggcttgttt ttctgttaaa cctacattgg ataaagtcgg agtgataaat
     3721 acactgtaag gcacagcttt ccggttattc gttgttcgtt tattctcgcc cttcaactga
     3781 tccataatga ttcgataatc atctagagag atataagtga actgcaatcc gcctttgacg
     3841 tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt attcatccac agcgattgct
     3901 ccgcgatctg ttagtttaac atctgtattt tctaatccaa gtgatgctgt acttggtttt
     3961 cttccagttg cagccaaaac ttttgacgct tggattgtta cttcttggcc atcttctgta
     4021 aaggtgacgg cagctgttga ttcttggtct gctacttgat ctacagatac acctagatgg
     4081 aattcgattc ccgcatttgt catgtcttct aaaatcattt gcgcgatatc ttcatcttca
     4141 cgtggaagaa attcagaatg ggcatcaaga acgacaactt tagatccgta ttcattgaac
     4201 attgatgcaa attccaagcc aatatatcca gccccgatga tcactaattt ttccggtaat
     4261 tgttcctgat ccatcgcttg cgtagaatca atcaagtagc gacttgtatt caatcctttg
     4321 attggtaaaa tgattggttc agaaccagta ttgatgaaaa tccgttcgcc ttcaacaatc
     4381 actttttcac cattattcgt cacttcgatt tgatggttag aaagaaaacg tgctgtaccg
     4441 tccatcactg tcccagttgc ctcatcgctg atcatatgat aatttttggc gcgaagcatg
     4501 cccgtcaatt tttctttccg agcaacagcc tctgtaaagt cgattcctct ttctccattg
     4561 aaaatcaatg atttagaagg aatacagccg atgttgatac atgttccgcc atacatacgt
     4621 gttgactttt caacgactaa aaccgattca ccttttgaag ctaacgcctt cgctaaagtc
     4681 ttaccgccct ttccaaaacc gatcacaata ttttgatatt tttccatcat ttttccctcg
     4741 cttctttttt acctaccgat tggtacaaaa atatgataac cgataattgt ctattctcca
     4801 aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc acgtttttag
     4861 tctggttcga tttcctttat tcttctaaac aatcgttact tctacatcga tatttcctct
     4921 agttgctttg gagtaagggc agaattcatg cgcatcttct gcaatttttt gggcttcttc
     4981 ttcgctgatg ccatcgatat gcacttctaa tactacaccg atctgatagc cgccatcatc
     5041 taaattataa agagagacac gtgcttttac tgtggactct gctgtcattc ctgcttcttt
     5101 gatcatatgt tgtaccgccc cattgaaaca agaagcataa cctgctgcaa ataattgttc
     5161 tggatttgtt ccttctgcat ggattcctgg aggagtgatt ttcatatgca tcgagccatt
     5221 tggtgcctcg acttctccat tgcgcccgcc atggttgatc attgctgttt cgtatgcttt
     5281 tttcattgta ctgctccttt cgattgacca tttgctactt ggctataatt atagtgtatg
     5341 acaagtaagg cgctttctac aaaaattctg cttattcgaa taccagaatg ccgtcttcta
     5401 ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata tttgttccaa
     5461 aataaatagc aatcttttct acataatctt ttatatgatt gattttaaga tacatatata
     5521 ccagaaagtt gaccccataa accaatactg ttaaaaaaat aaccatattc gttaccattt
     5581 tttcctcctt ttgctggtct ccctaccagc actcgattta accataccac tttttttgcc
     5641 gtccaagcaa ctagcctttc aaaaagactg gtttattctg ctgttaatga ttcaagatca
     5701 gaaaaatgtc cgaataattt tgctaatttt actaacgcca acttgttctt tccgaattca
     5761 tcattttcta tctgtaatac tagtaacggc tcatgtaagc ttaaacggat caaaaaccag
     5821 ccgtttccgt atggctcttc tagatcatat cgaatacctt cttcattttc cgggtcgaag
     5881 ttcatccctt tttcttcttc caacagtcgc ttcatttctt ctaatatctt gtttccttgt
     5941 tcgcgtggtt catcgctgaa caattttaat cggatttctt gtacttctac gggctgtttc
     6001 aactctttga tttgttcatc taatgtgata ccttttcttt ttaattctgg caaaagcatc
     6061 aggatctttg cgatgacata tgcaccatca tccagaaaat agttttcctt gaatgcagcg
     6121 tgtccgcttg tttcgatggc tagctgtgta tctttcccaa gacggttaag ttctaatgct
     6181 ttattgatta catttcgata gccggaaata taacgaactt ggatgcctcc tttttcttca
     6241 atgaatcttt gtagatgaga agaagtaggg gaattcgtca cgattcttgt tcctggatgc
     6301 tcttttaata cgattgtact taatactgcg atcaaattat ttcgattgat cactgttcca
     6361 gaagcactga caactgcaga acgatccaca tctgtatcga aaatgatccc aagatctgct
     6421 tcatttgcta aaacagcctg cttgatactt tccatcgctt ctttattatc aggatttggg
     6481 atatgattag gaaatcggcc atctggttcc aaaaattggg aacctgttga atccgcacct
     6541 aagctattca atactttttc agcaaagaat ccaccagcac catttccggc atcaacaatg
     6601 attttccaac catttagtgg tttgctttct agtgttccaa tccccttttt aatttttgtg
     6661 actaaatctt gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc agtttgttcg
     6721 ctttttattt tttgctgctg tgataggata aaagcaatat cttctttttc tgctcctcct
     6781 tctgaggtaa agaattttat tccattataa taatagggaa gatggctagc agtcagcata
     6841 accgcagcat cgcacgagaa ctgttcaaat tgagtggaca taaacatagc aggcgtagta
     6901 gctaattcga aatcgatgac atgtatccct tgttctttca gactttggat caacgcctct
     6961 ttcagtgctg gtccgcttat cctactgtca tgaccaatcc caatcgtcca ttttttattt
     7021 ggatcggttg atttcttctt tttctttatc cagtttgcta gtccccagcc gatctttttt
     7081 accatgtctg tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat tcctcgaata
     7141 tccgatccat tttgcagcgc tttcatatct ttcatataaa agtcctcctc atttattccc
     7201 tcattttttc cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg caaataataa
     7261 tgaatgtatc tacaccggcc atcgttccga caacttcagg gtaagccact tcatctagcc
     7321 aattcgttac cacatcggca ccatttcgtt cagtcaatac gataaccatg aactgaacag
     7381 actggatacg caaaacctcg cgtctaattg ctgtacgcaa acgttcttca tttaaagaaa
     7441 cagatggctg gttaaataat gtatagcgta ctattttgtt ttcattttga ctttttacta
     7501 acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg cctgcttcat
     7561 gcagccgcgc aagcagctct tcctgtgtcc caataggttg ttctgtaatg atctggcgga
     7621 tcagtgcttg tcgctgtttc tttttcatat tagacacctc tcaatgttga ttatagcaaa
     7681 agataagtaa tatttcttca tattttggta ttttttactt ttattttttg ttttcattca
     7741 ggttgctaac ctttttctat tttaacacgc aaaatcgtct tcattttttc aggtgctgat
     7801 tttcttggat cgctgatgta aatctctttg tgttctttag aagctctttc atagccttct
     7861 tttgcgatga atgcttctaa tttctcaaac gttgctggtt cttcggcata gggcccgacg
     7921 tgtaagattt gggcacaaag accttcttcc atctttttga atactagttg ttctttcaag
     7981 tcttccggaa gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc tgtaacaaag
     8041 tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt ttttcatcac tggttcatgc
     8101 aaatactttt cttgcagtcc ccattgtcct tccagtggat agacagtata catctgataa
     8161 tcaggaatca gccaattttt tttaggcgac atgcgtattg catagctgac tgcatataga
     8221 caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct gatgctaaag
     8281 aatggttttt taggaacctc tagtatttgc gggctttttt tcatactata gctcttgtct
     8341 ttacgccatt caattttttc catttttctt cctccgcgaa attccattta gttatttatt
     8401 atcatacctc tttttgagtg acttggctaa tcgtgttgac actatttttc agtttgtgct
     8461 agaataagtg aagtaaataa atcgtacaga taacacgttt cggcgtgtcg agagagaggc
     8521 ggtttctatt tttataatag aaatcactct ctttttttac gaaatttttt agaggtgatg
     8581 taaatgacaa aacaaaaaca gctgctttta gtacctgcag taattgcaac agggatgatg
     8641 tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa ctttcccaac tcttatcaga
     8701 cagtttgatt taacgactgc acaagtccaa tgggtaacaa cgatttatct tttgatgatt
     8761 tcgatcattg ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa aaaactcttt
     8821 ttagcttcaa atctgctttt cttggcaggt ataatgatcg atttcttctc tcctaccttc
     8881 atggtcctat tgtttggtcg attgctccaa ggtgcagcga caggtatcgc tcttccgttg
     8941 atgttccata ttatcttgac atttgccccc ctcaatcgac gaggcgcgat gatgggtgtc
     9001 ggtactttga caacggccat tgctccagca attggcccca cttatggagg cattatgact
     9061 tcacatttta catggaatca tatcttcctg tttcttgtgc ccgttttgat tctttctttg
     9121 attatcggct ggtttgctat tcccgagatg gcagtacaaa aaagtggtta tttggattgg
     9181 ataagcgttt taggaatttg tcttttgttt agtggaagtc taacattttt gagtacgttg
     9241 ggaacagttt ccagctggat tgctttaatc atcggcggta ttggctggtt ccttttttac
     9301 tatcgaagta aaaaagtgga gcatccactg atccgactgt ctattctttc aaataaaaca
     9361 ttccgcttgt ttttgattgg cttcttagtt tatcagtttt tattacttgg tgtatctttt
     9421 gtcttgccga atttccttca gatcgttcaa ggaagtagtc cttttgtcgc tggattagcg
     9481 atgcttcccg gcgcagcggt tggtgctttg ctttctcctt tttccgggaa aatgctggat
     9541 caatatggtc cgaagaagcc aatttttgct gggctgattc tttctttgat tggttggcta
     9601 gcattattcc tactcatagg acacgcttca gtgggtgtat tgattgcttg tcatgtgttt
     9661 tatatgatcg gtatcggatt atcttacagc aatatgatga ccaccggaat gaatgcactg
     9721 aatgaagaac tacaaggaga cgggaacgca atgttcaata ccttgcagca gttttctggc
     9781 gctgtggcta cttcattagt agcggtcatt atcaattacg tccagcatca tacttcccat
     9841 aactatgaag tgtctactac attaggatca aaagcagcat taggcgtctt gttgctgttg
     9901 cttctcgtaa gctttgctcg attcgcctat tatctgttct ttgctaaaaa agcgtagatt
     9961 ccatatacgg caggtgttgc atctgccgtt tttttgtgtg aaaatggttg tttgtaaaag
    10021 gaggcttttt tatggaaaaa attgccatta tcggtggcgg gatcattggt atgactctag
    10081 ccagttactt agatacatca aaattcaatg ttgctttatt tgatgaaggg acaggacagg
    10141 caactaaagc tagcgctggg attatctccc cttggttgtc gaaacgcaga aacaaacaat
    10201 ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa acttataaaa gatttccaac
    10261 ttgatcaaac gatttacgca caatcaggga cacttttgct tcgacctgaa aaagatttag
    10321 ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt ggaaacatcg
    10381 aatggctttc acctgaacag acatctcaag cacttccgtt attgaaacca gcagtttcac
    10441 tgcgtatcag tggtggtggt cgtctggatg gaaaagcata tttagaacat cttggtcagc
    10501 gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt cactttgcat aagaagagcg
    10561 attcttggct catcaaggga ccgtttggag aatacacagc tgatcgtgta attttgacac
    10621 ctggcccggc acttaagcct ttattacaag aattaggcta tgctgcagat atacgtccgc
    10681 aaaaagggca gctcttagca tttcagacat cttataagat gagtcaagac tggcctgttg
    10741 ctatgcttga cggagaagct gatttcattc ctttcatgga tggaaagatc cttttaggtg
    10801 ctacccatga aaacgatcaa ggctgggatt tggctcctac tagcgaagct tataagggat
    10861 tgactaagag tacagaacag ttcttgactg atccagatca gataaaaaac tggccatacg
    10921 agttccgtgt aggtacccgt gcttatacgt cagatttttc cccatttttc ggcgtactgc
    10981 cagatgatcc aagcttactg gctgctagcg gtctaggctc ttctggcttg accacaggtc
    11041 cttatatcgg ctatctatta gccaacttct taaatactgg tcattggtct acggaagatt
    11101 atcaaaagga catctccacc tatattacga aaaatccatc atagaaaaac agctttttca
    11161 agtaaggatc ccgagcgata ttgcgcatgg attacttctt gaaaaagctg ttattttagt
    11221 tcaatttctg aatcaaattc tccaaacttt cttttgcttc atctggcaag agatcttttc
    11281 ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc agatttttat
    11341 aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg atgtcttcaa
    11401 atggaccaaa cgacttgaat tctgccgttt gttgcacgat tctttcaacg attgacaatg
    11461 tcgtttcagg tttgattttt ttatcatcga aatatccagt attcaggaca tagcaggctg
    11521 tttgctgttt caaaaacaac tctcgaaaag cttgatagtc ttccctaaga ggatagacac
    11581 ggaaaggatt agcaaatggt tcaatgacta gctcatctag ctgtcttttt gtagtgatat
    11641 tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct aaaactgggt
    11701 cattgatctt gataattggc ggaagcgaat catctttcat gatccaaaaa acagcatcga
    11761 tcttttcgct aagatgatcg actcggtcgg gtgtcgcata acgtgatttg actgtccgcc
    11821 catttccgtt tcgtatatct cctgttacta ggactttttc tcctttactt gtacatgtca
    11881 cgcccacatt ctgacaagta aggaaagaag aaatggccgg atcatcagac ggatagtctt
    11941 gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa accatttttt cgagaaataa
    12001 cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt gctaaggtaa
    12061 tcgttgattt tccagaaccg gacaacccaa atacagccat cgtataggtc tggtcattac
    12121 tagcatattg tttcatccca ccgtggcagg caataaagcc attgcgattt cccaatgccc
    12181 aagcaagcgt caaagtcgct ttttttaatt ctccaaagta acgtaatcct agcacagccg
    12241 ctgtattatg gagagcatca aacaaaatga gtccatttgg aaatgtttca tgtgtccatt
    12301 cgggatcaca aaagatataa agatctccct catcaaagga aacagacttt tgataagtct
    12361 ctaagatctt cgaattcgct ggctgaaaat tcagcaaata agaatacaaa ttgttctcaa
    12421 atccttctgg aatcagcaaa tgactttgca cagaaaattc ttcagatagg ccaacgatta
    12481 cttttgtatg gtaaaactgg cgcttgcgac tttgatacac agcttctctt aaaattttct
    12541 cgtatttgct ttgttccttg tctaagtcct caaagatccg ccgagcactt gctgttcttc
    12601 ccacaatgct tccatcattg aatactagtg tcctaacatc agctggtaaa tctagttctt
    12661 cagcatgtga gattgttaaa tctgtgatga tcgttcctgg actccctttt gctagttgat
    12721 aagctgtctt cctgtcaaaa acttcatgaa cattattacc gtaaaatgtc gtttctacaa
    12781 tcgatcgaat actggataac agtggatttc cacttgtgat ttcgtttaat tcgagactct
    12841 cttttgtact catggctcct cctcattccc taaacaaaaa aacgttttct ttattataca
    12901 ttattcgagt ttttatttgt actgctgcgc tgtgggaagc aaacgagtta tttttcacac
    12961 aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc atcattttcc ttttatcggc
    13021 ttagtaccag ccgtttgcta accagaatgt tttagcagca tcccatgaac cataacgaga
    13081 tgttacatat tgttctgcta cacgttcttg attggcagct gaatagtcgc cgtttaagta
    13141 tgaagcatct aattgataac ggccgatgta acgtccgtta gtagctgtgt atgaaccgcc
    13201 tgattctttt tgtgcgatcc attcttttgc agagcttgaa gttggcgctg ttgcagcagc
    13261 tggggcagca ggagctactt caactgtttc aacaggtgca acgtatgtat gttcttgcgt
    13321 tgctgtttca gttgtagatg ctgtattttc tgttgttttg ttttctgttg ttgctgaaga
    13381 atcatttgaa gttgggattg ttaattgttc accaacataa atgcggttga tgtttgcgat
    13441 cgaatttttt tcagcaattg catcgatcat tgagttgtct cctgcaaatt tttgtgagat
    13501 cttagataat gaatcacctg attttactgt ataaacttca tcagcgtgag catttgtagt
    13561 tcctacaaac attgcagtag caccagcagc aaaagttgta cctaaaacaa ttttcttaaa
    13621 tgaattcata ttaaactcct ttttttctcc gttttttatt ctttcgacgc tattaacttt
    13681 atcatttaga aatatttcat aggtaaaaga aacatgacat ttaatgacaa aataacgaat
    13741 tatttaatat ttgtaacatt taaacaaata tttttttgat acaaaaccat aaaagccttg
    13801 ttgtataagc aaagtaatcg tttttctatg tttttttatt tttgaatgaa aaacgcagat
    13861 tctgtaacat tcactgcaat aatccataaa aaaaagcaaa aaaatttttg atttttatca
    13921 aattctctta aaaaaatgta aaaaatacaa aaaaaccagt aattgttaca acaattactg
    13981 gacaaaaact ttgaataatg ttaactattt ttttatttta actttctccg caataatttc
    14041 tgggcatgtt ccaattcgtt tataacgact tcttctgttt ctttagctaa tatttcatct
    14101 aatacatcaa gccatttttc cggttctttt gttcctagac gtccaatagc ccaagctgct
    14161 gttcctctga tgacagggcg gacatctgtt aaacattctg aaatctcagg caatgccgtt
    14221 cgatcaccta gattagctaa agcaatcaat gcgtttctct gcaaaggttt cttcccacgc
    14281 catgaaccag ctaaatgccc aaattgctgt ttgaagtctt tattagacat cgaaagcatt
    14341 ggctttaatt ttggatatac ttcgtctact tttggttcca tctcttcatg aaaatgaaaa
    14401 tcttttcctt gattgtaagg gcagactaac tggcagatat cacatccata aatgacattt
    14461 cgcatttttt tacgatattc ttccggcatc atcccttttg tctgagtttg ataagacaaa
    14521 catcgctggg cattcattct tccgtcacca agcaatgctt ttgtcggaca agcagtcacg
    14581 cagcgcatac agtcaccgca accaaattct cctggtttat caggctcaaa tacaatatta
    14641 gtaacgatct caccaagata gacaaatgaa ccgaactctt cagtgatcaa caatccgttt
    14701 cgtccaataa atcctaaacc agctcgctgt gctacagcga catcgatcag ttctccagta
    14761 tctacttgcg gagctagacg ccattcctct tctttttgcc atttttctgc tcttgtttgg
    14821 atgaattcga tcaatcggtt caaacggtct ctgagtatat catggtaatc gatcccccaa
    14881 gaagcacgag caaactgccc gcgtttttca tccctaggca cttcttcatg gatcctagta
    14941 ggatacgcaa gtgcaatgga aatgatcgat ttcggctgat caaatgtcat ttctggataa
    15001 agacgctcat cgatatttgg atgttcaaag ccagaagtat ggccagctgc tttttgttct
    15061 tccaacgatt cttttaatga atcaaaaggt tccgccgtgg tgaacccgat tttgtcaatt
    15121 cctaatcgtt tgctttcttg tatgatttct tcttttaatg tcatggtctt cctcgcttgc
    15181 tgttaacggc tgtataaccc ataatatagt ttttcttttt tcagctggac aaactggatc
    15241 tgctgatcgg caatcttctt ttcttccatc catttttttc ttgcagcaaa tacttcccaa
    15301 taatttgttg aaattaattt tcttttgtta tctaatgcat taaaaaaatt caatgaaact
    15361 ggttttgtac ttaacagttc atggagcctt acctcttccc atggcgaaaa ctcagacaga
    15421 tcgcgggaat acaatacttc ttcatagaga tttttttgct gtgtgtacgt gaatgtttgc
    15481 gggattttcc caggcctagc aatttctact agacgattat aaaacatatt cattaatttg
    15541 ctgttggcaa taaattgact tttataatag gccgactgga tctttttgat tactccttct
    15601 gctttgatga ctgaaaataa tgcaggccat gctttgacgg ttatttcgtc gttttgttta
    15661 actaatacgc caggtgtgaa gccgactgga gaaaggaaca caaaataacc gataccggta
    15721 ttttcatcta aaggctgaaa ttcatcaaac aaccatttgc ctatgtacag ctcttcatct
    15781 ggttctgttg tctcattttg atttgctatg aataatttag ctaattctgc ttcaagaaat
    15841 tgaaggtctt gatccttgtc ggtattttcc tgctggctga tcaatcgaaa gacttcttct
    15901 ttaatcgtat atttatcttt acgtgtaaaa ttcatagcca caccttcctt ccttactcta
    15961 actataacgg aatttcttca aaaaaggtat ctgtacgacg aaaaacagca taattttttt
    16021 tcatatctct tataaaaaat gcaaggtctg ctcctcaagc tagaccttgc acttttcagc
    16081 tttcttttgt ttgtgacaaa ccttcatgga tcgtttcaat attccatttc atcatctgat
    16141 agtaagaatc tccttgttct ccttcttttg ctatggaatc agtgaacagt ttatcataga
    16201 ttttcaaacc cgtttctttt gctacccgtt ccatgcttct tgagtctaca ctggtttcca
    16261 cgaataaaac cggtacttct tttgctctta tctgatcaat gatcgctttc atttgatcag
    16321 gcgtaccttg actttctgta ttgatctccc atatataagc tgctggcaaa tcatatgctc
    16381 gtgaaaaata cttgaatgcc ccttcacttg ttactaataa ttttttgttc tcttcgatag
    16441 aagcaaaact ttccttcgct tgtgtatcta attcttttag tttttccaca tatgcttctg
    16501 catttttttt atagagtgtc gcattttctg catctttttc agagaatata cgtgcgattt
    16561 cctccacata ttttattccg ttagataggt ctagccaagc atggggatct gcttttgtat
    16621 gttcttcacc gctagttaaa taaagaggtt ctacattttt gctaactgca aaataatctt
    16681 ttccttcttc ttttttagcc gtttccatca agttatcgaa ccagctgtta cctgtttcaa
    16741 gattcaaacc gttgtataaa ataacatcag catcgcttgc ctttttgatg tcttctggta
    16801 gtacttcata ttcatgcgga tctgttccga ctggtacgat actgtggata tctatttgat
    16861 ctgttccgac ctcttttgcc atgtccgcca agatcgaatt ggtagccacg actgacaact
    16921 tttctttact tccttcatcc gcttggtcag aagcctgctt tccgcaaccg gctaatacca
    16981 agaaactaac tgccaagatt aaaaagagtg atttttttgt tttcatctat ctgcttcctt
    17041 tctgaaataa aattcctttt gttggagaaa ataaaaaagc aaggataaaa aatgcggtac
    17101 atgctaacac gatcgtagct cctgatggcc aattgtaact ataactgaaa aacacaccga
    17161 caactgcact tagcataccg attcctgcag ctgtaatgat cattttcagt aaatggttcg
    17221 ttagcaagta agccgtggcg gctggtgtga tcaacatggc aatcaccaag attgttccta
    17281 ctgtctgtaa actgactaca gccactaatg tcaaaaagaa catcaaaaga taatggatca
    17341 gcgatgtgtt cagcccataa gcttttgcca ttgtcggatc gaatgatgtg atcttcaatt
    17401 ctttataaaa gaaaaagaca aatatcaaga cgattccact aacgagtgct gtcagcaaca
    17461 aatcgctttc cctaacagct aagacattcc caaataaaat atgatacaaa tctgtcgcac
    17521 tctgggcata tgaaatcaag ataatcccta atgcaaaaaa ggagctaaag acaattccaa
    17581 tcgctgtatc atttttcaat cggctccgct gagtcacgaa accaatcact gctgctgtta
    17641 gcatcccaaa agcagtggct ccaaagatgt aactaaaacc aaacatataa gaaatggcta
    17701 cacctggaag aactgcgtga gagatagcgt ctcccatcaa agacatccct cgtaaaataa
    17761 taaaagaacc gatgactcca gaagcaagtc ctacaagcat ggaagtgacc aaagcgtatt
    17821 gcaggaattg ataattcatt aatccatcaa taaaggattg gatcattttt tctctcctcc
    17881 ttttactaaa tgtctgatga tctcaccata ggccaattga aggttcttcg atgtaaagac
    17941 ttcttgtact ggaccggaag cgatcagctg tttatttaaa agaataacct catcgaaata
    18001 ttcttctact ttatgaagat catgatggac gatcaaaatc gtttttcctg aattcttcaa
    18061 ttcctgcaag atgtcaaaga tctttcgttc acttaacgca tcaatcccta cgaatggttc
    18121 atctaaaaag atccattctg ctccttgggc tagagctctt gcaataaaaa ctctctgaag
    18181 ctgtccaccc gatagttcgc tgatctgtct tttggcatat tcttccatcc ctactttttt
    18241 caaagcttgt cttgcacgtt ctttttcttt tttcccaggt ctttgtccaa ttcgtaaaga
    18301 tggatatgtt cctaaaagta caacgcctaa tacatcaatt ggaaaggaaa gatccaattc
    18361 acttcgctgt tccacgtagg ctattttttt acggactgag tttaccggct gtccagaaaa
    18421 ggtgacttta ccagtagtag ctggtatcaa ctctagcaaa gatttcataa atgttgattt
    18481 tcctgcaccg tttggtccga taataccggt aattttttgt ggatttatcg tcaagttgat
    18541 agttttcaaa acagttttcc cttgataagc ggctgaaagg tcttgaatta caatggccgt
    18601 acgttccaaa tcttacactc ctttttaggt ttacctaact ttttatttaa atatagttta
    18661 actaaaatgg aaagcatttt caagtagaat gatgaaaaag tttttttcga attttcctcg
    18721 cattttatga aaattttctg tagaatcaca tcaagagttc aataaggcta acctaataat
    18781 gaaaaacatg tgttgaaacg tttgattatt ttttcttgtt gactcaacaa cgaaaggagt
    18841 ttttgtatgg aagaagaaaa gttaactcga agtcttagtg cccgtcatat ccaaatgatc
    18901 gcactaggtg gaacgatcgg tgtcggtctg tttatgggtg cctcctcaac catacgttgg
    18961 acagggccct cagtgatggg agcctatgcg attgcaggat tctttttgta tttgattatg
    19021 cgtgctttag gcgaaatgct ttacgttgat ccttcgactg gttcttttgc aaattacgcg
    19081 agcgagtaca tccatcctgt ggctggctac ttgaccgctt ggagtaatat ttttcaatat
    19141 attgtcgtag gaataagtga agtgattgct gtaggatctt atatgaatta ttggtggcct
    19201 gatttgccgg caattattcc cggaatcatt gtcgtcttgt ttcttatgct ggcaaatctt
    19261 atttctgtca aggcgtttgg cgaattagaa ttttggtttt cgatcatcaa agtcataacg
    19321 atcattttga tgatcattgc aggtcttggg gtaatcttat ttggatttgg taatcatggt
    19381 caggcagtag ggatttcaaa tctttggaaa aatggtggat tcttcactgg aggcgtcaaa
    19441 ggatttttct ttgcattatc gatcgttgtt gcttcctatc aaggaatcga actgatcgga
    19501 atgactgctg gtgaagcaga gaatcctaaa aagacgatca ttgaagctgt ccagtccact
    19561 atcggtcgaa tcttgatttt ttatattggt gctattttta tcattgtgag tatctatccg
    19621 tggaatcaat tgagtgagat tggttcacct tttgtacaaa ctttttctaa agtcggcatt
    19681 acatttgctg caggactgat taattttgtc gtgattacag cggcattatc aggttgcaat
    19741 tcagggattt tcagcgcaag tcgtatggtc tatacacttg ccataaatgg aaagatgtct
    19801 aaaaaatttc tgaaactatc tcgtcacggt gcgccttttt atcctgtcgt agcaatctca
    19861 ggtggaatct taattggtct gatcttaaat tatcttctac caatgttcat caaccagtca
    19921 gaaaatattt tcgttttcgt atacagctca agtattttgc ctggtatggt tccttgggta
    19981 gtgattttga tcagcgagat tcgcttcaaa aagaagcacg cagataaaat gcatgatcat
    20041 ccttttaaaa tgccactatc tccttatacg aattatgtga cacttgcttt tttagctatc
    20101 gtattgattt tcatgtttat caatcctgaa acgagaatct cattgattat tggactcgtt
    20161 ttcttgcttt acatgacgat ccattacttt gtacgcgaaa aaagaaccgg aaaataaaaa
    20221 aagaacgaag cttcacctta gtggaagctt cgttcttttt tattgctttt atcaattcaa
    20281 ttttagcgtc tcaccgggag tcaggaaata attctccatg ctcatcccat tcagcgacaa
    20341 gatcgtttct actggtacgc cagttcgttc ggctatttga tttggacctt ctccttccaa
    20401 aactacggtt gtcgcagcct gtgaagtcgc gctgttggta ttagtttcag aggaatctgt
    20461 tgtgtcttgt gttgttcgag cttgcatttc agaagcttct accgactctg ctgtctcaga
    20521 atcagaagat gttgtgtctt gtgtgcttgt tgactctgct tgaagagagg cgctagtctg
    20581 caaataaata tcggaaatct tagcgacatg attcgtataa ttagccgata attgattgat
    20641 caaagtatct aagtctacac cgtctttatt ttctgctgcc tgatacgctg cacgtaattt
    20701 tctgatccct tcgtcggaga ttgcttgaag ctcacgtgct ttttggttag caactgctga
    20761 taaaccagct attccctctt gatttccctg aatttctgtc tggtattctt caatcaatgc
    20821 aggcgtcttt tcttcgatct ttttcgaata atcatttacg attgcttgaa gttgctctgg
    20881 caaaccattt gctgtttttt cttcagaagt ttgtgtctct tttgttttag ttgatgactc
    20941 acttgtttgt ttcgtctctg ttttctttgt cgttgtttcc gttatggtcg tacttttcgc
    21001 ttctgacgaa ttttttccat gaaaatatag acccgcaact cctcctgcta tgatcaaaaa
    21061 cacggcaact gctgccccaa tcatgatcat ccgcttacga gctgcctgtt tttgctgtct
    21121 tcttcgggtc atccgaacat tttctcccca ctcattttca tcatacattt ttttcaccac
    21181 caatttttat gtataatagc gttatcaaac gcttcatatt cttattgtag caaataattg
    21241 tcgctgtgca catttttatc tttgttattt ataagaaaat ttcaccagtt gagatgattc
    21301 caagattgtc cgtcgatttc tccttgcagc tgcattgttc gttcgtctat ctcagccatc
    21361 agtttcttta tttcatataa gagcgcacgg tcttgatatt ctgatttttc gcttaattca
    21421 tctaattgtt ggatcagcca atcatttgtc ataatgaagt tcctttcatt tcttgcagtg
    21481 taaaggtcaa gtcttcgatc tgctcttttg cctcagtcag tgcttctacg atatatgctt
    21541 gtttttcaga ttcttcttcc tgcattttct gattttcctg tactctttga ataaaccagt
    21601 caatccgctg agtcagcgat ggtttgcctt ccaatgagag atactgctga taagcaatta
    21661 gcttctccca acgtttttcc tgggataaat acataaattg tgctaaaacg aatggcggaa
    21721 gataggtcat atggaattta tttgcgattc gctgatacgg cctcaaaaat tcggaaatcg
    21781 tatattcttc ttttcctcca gcttggtatt ctttttccgc cacacctgtt gtcaatacga
    21841 ttcctagttc tttccctttt aatacatgtg ctgctcgttc caagacatta tcttgccaaa
    21901 tttttaaatg agcaggagaa ctataccagt aaaacggaaa ctggaaaatg atccgatcat
    21961 gctgggccaa gagctgtagt tcagtctcta tatctatttt tccgtctgga taacttgatt
    22021 ccaaatgatg ccaagtagct ggtaaaccag cagaagcttc tttgaaaaat tgctgattta
    22081 aagattcttc gatcgttgga tggctaatga tgataagtgt acgcatccct tttcctcctt
    22141 tttagtcttc gctttacagt gtagcataac cgccacaagt ttcttttgcc gatgcttgtc
    22201 attttaacca gactaatcag gttgtccaag aaactgcggt ttgtttttcc aactgttttt
    22261 tcaaggtatc caaagcaata ttcttaaaat tttcgttttt catacgttta cgatagttcg
    22321 tagccgtttc tccggtactc tttttgaaca ttcggctaaa tacgactaga ctattgaacc
    22381 ctacttgata ggcaatactt tccacggatt catccgtcag agaaagaaga aacgcccctc
    22441 gattgatccg taccccgatc aaatattcct tgatactggt acccatatga tttttgaata
    22501 ctcgcgacaa atggctccga ttgaccgcta aatgctgggc gatttcctca acagacaatt
    22561 tttcagcata atgtgcctcg atgtatttga ctgtttcaac agctaaagag gaatgaacct
    22621 tttgtacacc accgctgacc tttccgccat cttttaaaag gactgataaa aaacggtagg
    22681 ttaatgctgt taactgtaat tcattttcca aggtttcttc cgtatctttc atgcattcta
    22741 aaataatgtc gatatatttt tgtatgtcag aagagaccat cgtgtattgg tcttctctga
    22801 aaagcgactg ccgaatgatt tcttctgcag cactcccacc aaatgaaagc cagcaataca
    22861 tccacggatc ttctccatct gataagtaaa acgtcgagtc tcctggacga atcaagaaca
    22921 gatcaccttt tttcaattgg aattgttgat ttttcacatg ataagatcct ttgccttcca
    22981 tcacgacatg caggatgtaa tcattgcgaa tagcaggacc aaaagaatga aacggcatcg
    23041 ttttagagaa accacaaaaa tcaaaataca attctttttc tacttgttga tgttcctttt
    23101 ttaaaaaaat cgattgctcc acgtctatcc ctccttactc ctttagtata atgcaacaaa
    23161 aagttaaata aaagtcacag aaatatatta aaaaggaagc gatttcttgt taagtttaag
    23221 tcattaaaag atctttgtta ttcgttgtaa ccggtttact tagtattata tattgaacag
    23281 gagaggaata cagattatga aaagcaaaat gatccaacgt gtgtcctacg ctttaggcgc
    23341 ttttggacac gacacattct atgttacatt aagcacttat tttatggtat tcgtgacaag
    23401 ccagatgttc gcaggaacag ataaggcaac gaatgcaaaa atgattgcta cagttacaag
    23461 cttagttgta ggtatccgct tagttgaaat tatttttgat cctttgattg gtggcatcat
    23521 cgataatact cgtacaaaat acggtaaatt caaaccatgg ttagttatcg gcggcgttgt
    23581 tagttccgta ctcttggtag caatctttac caatttcttt ggcttagcta ctcataatac
    23641 gactttattt atcatattat ttgcaatcgt tttcatctta ttggatagtt tttattcatt
    23701 taaggatatc gctttttggt caatgattcc agctttgagt acggaaacaa aggaacgtga
    23761 aaaatttgct acgtttgctc gtttcggctc ttctctaggt gcaaatggta ctactctagt
    23821 cgttgtccct atcgttactt tctttaccta tcttgttact cataaacata ttgaaggcgc
    23881 ttcaggctgg ttctggttcg gtgtgattgc tgctgttatt tcaggcggta ctgcgattat
    23941 cactgccttg ggcacaaaag aagtcgatac tgcgatccgt cagcaaaatg aaaaagctgg
    24001 gatcaaagat gtgttcaaag cagttgctca aaatgatcag ttgatgtggc ttgcccttgc
    24061 ttatatcgct tatgcgattg ccaatgtagc aacaactgcc gtattattct acttcttcaa
    24121 atatgtactt ggccaaccaa ctgaattttg gatcgttggt ctagttgctg ccttacttgg
    24181 tttagtagct gttccactat tcccaatgct tacacgcgtc atctctcgtc gctacgttta
    24241 ccttggaggg atcgcaatga tgatttcttc ttactttttc tttacgatcg caggttcaag
    24301 tttgccaatc gttatcatcg ggctagtatt gttctacttc cctcaacaat tgattttctt
    24361 atccgctttg atgacgatca cagactctgt tgaatatggt caatggaaaa atggtatccg
    24421 taacgaagct gttactctat caattcgccc tttactagat aaaatcgctg gtgctttgtc
    24481 taacgggatc gtagggtttg tcgctatcgc ttctgggatg actggtaatg ctactgctgc
    24541 tgatatcact gctcatggcg taacgacttt caaagcttat gcattctatg ctcctgctgc
    24601 tttgatgatt ttctccgcta ttatttttgc ttggaagatt acattgacag agaaaaaaca
    24661 tgcacaaatc gttgaggaat tggaaaacaa attggctcca gctgatacaa tggaagatga
    24721 acttcacgat gcagtagttc cttcccatta agaaaggatc ttgaaaatga agattacaga
    24781 aaaagatttt ggccaaggtt atcacttgat aaccttggag aacaaaaaca agctagcact
    24841 ttctatctct gatttaggtg ctagaatcgt cagtttaaaa tcaaatgatc gagaattagt
    24901 tttgggattt gacaccgctg aagaatatat cgaaaaagat ccgtatatcg gtgcttctat
    24961 cggtcgcacc gcagggagaa tcgaaaatgg ccgcttttct ctcaatggaa aaacttacca
    25021 attagccaca gatccaaaaa ctggacataa tctgcatggt ggtgctcctg gatttgaatt
    25081 aaaaaaatgg tcttatgtca ttttgaatgg cgaaaatgaa gcttctgtca tttttactac
    25141 tacaagtccc gacggagaac atggttttcc aggtacaatg gatgtggaaa tccgctatac
    25201 tttgacaaaa gataatatct ggcgagtgac ctctcgcggc accagtgacc aagatacttt
    25261 attcaatccg acaaatcatg tttacttcaa cttgacggga gatgcttctc agtcaatcga
    25321 tcaacatgag ctttggctca atagcgaagc ttatgctcct ttgcgaacag atagtattcc
    25381 tataggggta aaagaaaatg ctgcaggaag tgctttcgat tttcaaatac ctaaaaaatt
    25441 agcgtccgtt tttgctagtg atttggatca aaaaaatctt gttgacggaa tcgatcatcc
    25501 atttttctta aaagaaaccg gcttgggtaa agaagccgct cgcttgacta gccccgataa
    25561 aagaatccaa gtaggtattg ctacagatgc ttctagtgtg gttattttca ctgcaaattt
    25621 cggcacagaa acaccagaaa tgcgcaaccg aaaactggca catcacggtg gcatcacatt
    25681 tgagactcaa acagctccag gtgcagagcg cttctcagca tttggttcga ttcatttaaa
    25741 agctggatca gtatttgaaa cagtgacaga attcaaaatc aaaacaagaa aggaataact
    25801 catcatggaa atcaaaagaa gcttaaacac aaaatttgcc gaaacattcg gacctaaaaa
    25861 aactacacaa tacttctcac caggacgcat caatttgatt ggtgaacaca cagattacaa
    25921 tggaggccat gtcttccccg cttccatcac ttatggaacg caaggtgtag cagcacctcg
    25981 agacgataat aaaatattag tttattccac aaactttgac gatatcggtg tcattagttt
    26041 tactttagat gagctagaat atgataaaaa agcaaactgg gcaaattacg taaaaggaat
    26101 ggtccttaaa ttaaaagaag ctggacactc aatcgatcat gggtttgagt tgttagtgga
    26161 aggaacaatc cctaatggtg caggactttc cagtagcgct tcattagaat tattagtagg
    26221 tgttgtatta gaagatctgt ttgatttaaa aacagatcgc ttggaactag tccaaacagg
    26281 taaaaaagta gaaaatgaat tcatcggtgt caattcaggt atcatggatc agtttgccat
    26341 cggcttcggt gagctagatc atgcaatctt attagataca aatacgctga aatacgaaat
    26401 ggttccagtg aaactagatg gttatgcaat cgtcatcatg aatacgaata agcggcggga
    26461 attggcagat tcaaaataca atgaacgccg tagcgaatgc gaagaagcct tggcacgttt
    26521 gcaaaaaact ttgcctatct cttctttagg agacttagat gaagaagaat ttgaatcaac
    26581 tattgaccaa atcggcgatg atacattgat acgcagagct cgccatgccg tttctgaaaa
    26641 tcagcggact ttgaaggcaa aagctgaatt agaagctggg aacttagaag catttggtca
    26701 actgttaaat gattctcacc actctcttcg ctatgattat gaagtaactg ggatcgaatt
    26761 agatacacta gtagatgctg ctcaaaaaca agaaggtgtc ttaggtgcac gaatgacagg
    26821 agctggattt ggtggctgtg cgattgctct tgtcaaagaa gaaaatatac ctgccttcaa
    26881 aaacaatgtc tatgatgaat acatgaaagt tgtcggctat gctccggaat tttatgttgc
    26941 acacattgga aatgggacaa caaagatcga tccagaagct gttcatgttt gataaaaaaa
    27001 tcaacgatct cagctaaaaa caaatagata aaaataacct ctcagcaatt tccaaatgtg
    27061 agccttttgc tcgtatttga aaattgctgt tttttattga cacggaaaga gaacgtgtgt
    27121 tctaattgtg ttgaagaggt gattgttatg tgggcgaatc aaaatccggt atttgtttat
    27181 gaaaaagaac cttcaagaga tattttatgt attgactgca aatcttttta cgcctccgta
    27241 gagtgcgttg aaaaggatct tgatcccctt acaactaaat tagtagtcat gtcctatccg
    27301 tccgattcga cagaaacacg gggtagcgga ttaatcttgg cttccagtcc tgcagcaaaa
    27361 aaagcttacg gcatcaccaa tatcagccgt gccagagatc ttcccttccc ctatccaagt
    27421 gatttatata ttgttgcccc aaggatggcc tattatatgg aaaagaacag agaaatcaat
    27481 tctatctata aaaaatatgc agacgaagcg aaccatcatg tctattccgt cgatgaaagc
    27541 ttccttgatg taacaaatag catggcactc tttggtaaaa caaatgcgta cgacatggct
    27601 aaactgatcc ataaagatgt gtttgagcag accggaatct acacgactat aggaattggc
    27661 gataacccgc tacttgctaa actagcctta gacaatggct caaaagacgg aaagcaaatg
    27721 tttgctgaat ggcgttatga agatgtacca gaaaagttat ggaaaatctc agatatcact
    27781 gacttttggg gaatcggcaa acgaacagca aaacgtctga aacaaatggg tatacattct
    27841 gtttatgatt tagctcacgc ggattattat cacttgaaaa gcaagctagg ggtcatcggt
    27901 actcagcttt atgcccatgc ttggggcatc gatcgaagtt ttttgggaca agaatataca
    27961 ccaaaatcaa aaagtatcgg aaatagtcaa atcctagata gagattatac aagacgagaa
    28021 gaaatcgaga tcgttatcaa agaaatggcc gaccaagtcg gcactcgctt gagacgatct
    28081 gcagcaaaaa cacaagtagt cagcttatgg gtaggatact ctttaggcta tcttgatcca
    28141 aatggcaaga ccgggtttca ccagcaaatg aaaatcgaag cgacaaatgc tagcagagat
    28201 ctagcagatg ctttattgac cattttccac cagcattacc actaccagga tattcggcgt
    28261 gtaggcgtga actgttccga cctcgtatac gcgacaggtt tacagcttaa cctttttgaa
    28321 gatcctgaaa agcagataaa tgaagcgaaa gttgattttg ttgtcgatac gattagaaaa
    28381 aaatacggct ttcaatcaat catccatgcg catagtctct tgcaaggagg acgcgccatc
    28441 gcacgtagtt ctctcgttgg cgggcatgca ggtggtatga gtggattaga aggtggaaaa
    28501 gatgagacgg acagcaaaaa aattcattga ctataacgag tatcgagatc ggccatttgg
    28561 attaaaatgg gggactgctt ttgcgatgga agaactagtc aacggtatca aaaaaaatga
    28621 agaagaagca cgcaaacagc ctcttcctaa acaacaaatg acattgcaag aaatcgatag
    28681 tatactgaca gaagcctttt tatctcgtaa agcagtaatc attcagctca atttaaaaga
    28741 tgaatttgga agactcttgg atgaggtcga agggcacttc attggtgaag cctatgaaga
    28801 ttactttatg attgaccatc agccgatatt gtgggaagac gtacggtata ttgagttaaa
    28861 ggaaacaaaa aaatggtttg tgacatgaga ggttgtgaca gaagtgatca acttcaaaaa
    28921 ataagaaagg atttttcgaa aattgcttct taaatttttg tggaattttg gcttattttt
    28981 cgaagaagca acttctggaa caacgtatat tagatatcag aggttgtgaa aaagtcgctt
    29041 agttttgagt aacaagccaa ggcgctgatt tcacaacctc tgtctatcta tccatctatt
    29101 cggtaagatt catgcatgac gtcttcttta tttagtatgt agagcccttt ttcttctgac
    29161 cattcaacat aaatcagctg ttttacatct gcatagcctt ctttcttcaa gttctccata
    29221 agccatgctt cagtcaattg attggactga agcactttat gctgaatttg cccttcatct
    29281 acaagcagga tcgataaagc tttgtcttca tctgattttt tcaatacact catttgacca
    29341 gcattctcta ggataacatg tttggcattt tcaagagaaa agcattcttg ctgtcttaag
    29401 attgctcgca actgctccat ctcgatatgg ttatttgtca actctgtcat gttgatgacg
    29461 ccgtctttga ttagaacaga tggctcgcct ttgacccaac gattgacctt ttcatttttt
    29521 tggacgatac gttcgatacc ataaatcatt actgcccaaa gcgcaatggc aaataggaca
    29581 tgccctacat ggacattgtc atcataggtt gattcttcta agatcccacc taaaacaagc
    29641 gtatagacta aatcaaacgg tgtgatttct gacatggatt tcttcccaag caaacgtacc
    29701 actaataata aaccgatcaa cccgaacacc agtttcattg taattgaaaa aatcattttt
    29761 tctcccccta actatctgtt aattaaatta tatatgcttt ctcacgaaca cgcatggaat
    29821 atacaaggaa gaaatgtatc cttttactaa aaaaaggact gtgacactgt agtgaccccc
    29881 aaaagttaga cttttttgga gtggagaaat ccactccttt tttttatgct ctaaaatgat
    29941 atttttacac aaccgttacg gttgttttac gaaattggac gagactttgc cagtttaatt
    30001 tctgttttat ccgttcatta ttgtagtaat agatatagct atcgatggct gattttaatt
    30061 cctcgaaaca gttataaact ttcccgtgaa aaatctcttg ttttaataag ccaaaaaaat
    30121 tctccataag tgaattatct aagcagttcc ctttccgaga catgctttga aagattttgt
    30181 actccttcaa tttgtttcta taagctttca tttgatacgc ccaaccttga tctgagtgaa
    30241 acgtacgacg atagggacaa tcgttagtgg tctggatagc ttcctccaaa ccttccatta
    30301 ccgctaatgc attgggcttt tcagagatac gataggataa aatctcacta ttgaacatat
    30361 ccataaaagg atctagatac aatttctttt gacggataat gcctttggca tccacttcat
    30421 ggtatttaaa ttcagttgta tccgtcgtga ttttctgatg gcagatactg gtgtagaaac
    30481 gacgatggat
//
LOCUS       DAJSAW010000030        30082 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJSAW010000030 DAJSAW010000000
VERSION     DAJSAW010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.030"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..570)
                     /locus_tag="ONT64_001548"
     CDS             complement(<1..570)
                     /locus_tag="ONT64_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2302971.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYN"
     gene            complement(647..1177)
                     /locus_tag="ONT64_001549"
     CDS             complement(647..1177)
                     /locus_tag="ONT64_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCR2302972.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1181..2041)
                     /gene="rlmB"
                     /locus_tag="ONT64_001550"
     CDS             complement(1181..2041)
                     /gene="rlmB"
                     /locus_tag="ONT64_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR2302973.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2034..2429)
                     /locus_tag="ONT64_001551"
     CDS             complement(2034..2429)
                     /locus_tag="ONT64_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCR2302974.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2426..3838)
                     /locus_tag="ONT64_001552"
     CDS             complement(2426..3838)
                     /locus_tag="ONT64_001552"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR2302975.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3835..4371)
                     /gene="cysE"
                     /locus_tag="ONT64_001553"
     CDS             complement(3835..4371)
                     /gene="cysE"
                     /locus_tag="ONT64_001553"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR2302976.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4457..4696)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4693..6153)
                     /locus_tag="ONT64_001554"
     CDS             complement(4693..6153)
                     /locus_tag="ONT64_001554"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR2302977.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6323..7480)
                     /locus_tag="ONT64_001555"
     CDS             complement(6323..7480)
                     /locus_tag="ONT64_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCR2302978.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7514..8884)
                     /gene="radA"
                     /locus_tag="ONT64_001556"
     CDS             complement(7514..8884)
                     /gene="radA"
                     /locus_tag="ONT64_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR2302979.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8947..9474)
                     /locus_tag="ONT64_001557"
     CDS             complement(8947..9474)
                     /locus_tag="ONT64_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR2302980.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9704..10510
                     /gene="proB"
                     /locus_tag="ONT64_001558"
     CDS             9704..10510
                     /gene="proB"
                     /locus_tag="ONT64_001558"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR2302981.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10507..11754
                     /locus_tag="ONT64_001559"
     CDS             10507..11754
                     /locus_tag="ONT64_001559"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR2302982.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11830..12174
                     /locus_tag="ONT64_001560"
     CDS             11830..12174
                     /locus_tag="ONT64_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302983.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12291..12617)
                     /locus_tag="ONT64_001561"
     CDS             complement(12291..12617)
                     /locus_tag="ONT64_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2302984.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13232..14434)
                     /locus_tag="ONT64_001562"
     CDS             complement(13232..14434)
                     /locus_tag="ONT64_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2302985.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14551..15066
                     /locus_tag="ONT64_001563"
     CDS             14551..15066
                     /locus_tag="ONT64_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR2302986.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15265..15711)
                     /locus_tag="ONT64_001564"
     CDS             complement(15265..15711)
                     /locus_tag="ONT64_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCR2302987.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15758..17236)
                     /gene="msr(C)"
                     /locus_tag="ONT64_001565"
     CDS             complement(15758..17236)
                     /gene="msr(C)"
                     /locus_tag="ONT64_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCR2302988.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17590..18219)
                     /locus_tag="ONT64_001566"
     CDS             complement(17590..18219)
                     /locus_tag="ONT64_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR2302989.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18203..18667)
                     /locus_tag="ONT64_001567"
     CDS             complement(18203..18667)
                     /locus_tag="ONT64_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2302990.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19154..20422)
                     /locus_tag="ONT64_001568"
     CDS             complement(19154..20422)
                     /locus_tag="ONT64_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR2302991.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20653..21390)
                     /locus_tag="ONT64_001569"
     CDS             complement(20653..21390)
                     /locus_tag="ONT64_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2302992.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21457..22026)
                     /locus_tag="ONT64_001570"
     CDS             complement(21457..22026)
                     /locus_tag="ONT64_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCR2302993.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22154..23026
                     /locus_tag="ONT64_001571"
     CDS             22154..23026
                     /locus_tag="ONT64_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2302994.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23368..24240
                     /locus_tag="ONT64_001572"
     CDS             23368..24240
                     /locus_tag="ONT64_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2302995.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24237..26195
                     /locus_tag="ONT64_001573"
     CDS             24237..26195
                     /locus_tag="ONT64_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2302996.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26527..27378)
                     /locus_tag="ONT64_001574"
     CDS             complement(26527..27378)
                     /locus_tag="ONT64_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2302997.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27501..27968
                     /locus_tag="ONT64_001575"
     CDS             27501..27968
                     /locus_tag="ONT64_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCR2302998.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28231..28713)
                     /locus_tag="ONT64_001576"
     CDS             complement(28231..28713)
                     /locus_tag="ONT64_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HCR2302999.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(28813..29676)
                     /locus_tag="ONT64_001577"
     CDS             complement(28813..29676)
                     /locus_tag="ONT64_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR2303000.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         8582 a         6367 c         5452 g         9681 t
ORIGIN      
        1 attataaaat ttcaagtgtt tttttatttt gagttttact ctcccgtaag cgctcattac
       61 ttgcttgtga tccattgcta aacattcagc catgtctaca aaatcaacgc cttgcatata
      121 ataatgaaaa attttagctt ctatttttga aaactggatt tcgcaatttt cgattgcttc
      181 ttttaataaa agatgctgaa ttgctaaagg ttcttgcaat gtgatctctg ctaaaaagtc
      241 tggtccttct gtctctattt tttggtcgat tgagacttca tctttttgat cacgacgctt
      301 cagcgcgttc tgcttcctca acagagaata gatttgattg cttataacgc ttttataata
      361 acttccaaag ctaacacctt tgcttttttc gtaagttctc aatgcctgat ataaagcgat
      421 ccttgcttct tgttcccaat cctcagaaac ataatttctt aaataatagg tgtttttcaa
      481 acgtagtaaa agtggaaaat atttttgata cagggcagca aatgcttctt cgtcaccatt
      541 acgtgcagcg cgcaataact cctcagacat aaaataattc ctccataaat tcgtttatgg
      601 aggaatctta tcatcaatga ttcatcgaac tgattagaac gatatgttat ttattttttc
      661 tcattaaatc gtccaatttt tgggacaatt tttttaactg ctcttcattc catggagaat
      721 tacgtcggaa atcaataaaa tgcatatctt tattttcttg ggctattgtt ttttccgttt
      781 tttttatcgt cttatataat tcataagcag aagttcgtaa tgctccttgg gaaaagatca
      841 tccactgttc tgctaaatcg cttgtcgcca ctgtcacttg tgtcaaacgg tcattcagct
      901 gaccagcgat tctctcgata taactgtcag ccgtttcgtc ttctttcgta aaaacaacat
      961 ccaactggta tttcttataa ttttgttgga tgcccggaac tagttgggca tcgaagacaa
     1021 cgatgacttc cattccttca tatttcgcat aatttgataa acgatgtaaa agagcttctc
     1081 ttgcatccgc cattttgtcc tgctttttaa gctggacaag ttccggccac gaaccaatca
     1141 tgttgtagcc gtctacaatc aacagctgtt ttttcatggt ctactccccc tgacgttggg
     1201 aaaaagcctg atacataagc aaaccagcag caacactggc atttaagctc tggacgtgcc
     1261 cggtcatcgg gatcgtcagc aattcatcca tttctttttt caatcctgga ctcattcctt
     1321 tgccttcatt accaatgact aaagcaattg atcctttggc attccatttt tggaagtttg
     1381 tgccggtcat atctgtgcca aatacccaaa atcctgtttc tttgagctgt tgcacagctt
     1441 gtaccaaatt cgtcacgcgt gcaacaggga catgttcgac tgccccagtg gaagtctttg
     1501 ttacgatagg tgtgattcca actgctcggt gttttggtat gatcacacca tcgacgccac
     1561 ttgcgtctgc tgtccgtaaa atcgacccaa aattgtgtgg atcttcgatg ctatccaaaa
     1621 tcaaaaagaa ggggtcttct tttttctctt ttgttcgttc aatcagttgg tcaagggata
     1681 aatattcata aggtgtaatc gcaagaacga ctccttgatg cacgccgtga tcgctcaaag
     1741 tttctaattt ttgtttagga acccatttga ctggcacagc ttgttcacgt gcgatttctt
     1801 tcagttcttc tactttttct cctcgtgaat cttcttgaag aaagagttta ttccctcgcc
     1861 cttgttgtag cgcttctact gcggcatggt gcccaaagac gaaattatct tctaatgctt
     1921 cttcattttg atcagaaact gactgatttc gtccagtttg ctttttattt gaatatctat
     1981 tttgtttttt gtcttcaaat ccacgtttcg attgatgttt gtttgttctt tctttaccca
     2041 tttttttcac caactttctg aatacaccag cggatcaatt cttccatccg ctcggtttgt
     2101 tttgtcaaat gcagatagcc catgagcgct tcgaaccctg ttgcgacccg atacgtcgta
     2161 acatcagcat tttttgcgct cgtatggctt ttagcatttc ttcctctgcg ataataaagt
     2221 tcttcttctt ctgtcaaaag atgttcttcc agcatttgct gaatcaaaaa ggcttgtgca
     2281 ttcgcagaaa cataattcgt tgccgcacga tgcagcttat tgggttttgt ttctccttga
     2341 tcaatcaggt ggtcacgaac atagatttcg taaatcgcat ctccaacata agccaaggct
     2401 aaaccgttca attgtgtgta atctctcatg cttctcttct ccatcgggtt ccttgcggtg
     2461 tatcttctaa taagatccct ttttctttca acaaatcacg aatttcgtcg cttcggacaa
     2521 aatcacgatt gcttcttgct tgatttcgtt cttcgatcaa ttgttcaatc tcttcatcta
     2581 ataactcttc tgctgtaaat aaaataccga agatcgaaag ccattgcgag aataaagctt
     2641 ctccttgtgc caagacagcg gcagaaactt tcggctggtc tagataagtg ttcagccact
     2701 tagcaagctc atagaccact gtgatcccat ttgctgcatt aaaatcatcg tccatttctt
     2761 cgatgaatcg ctgttctaaa ttttctaaca aagcgatttt atccgcatca tctgccaaat
     2821 tctctgaggc agtttcttgg cggaatttca aattgtcaaa cgtattcttc agtcgttgta
     2881 aattggtggc tgcttctttc atcgtcgttt cactgtagcg gatcggacga cggtattgtg
     2941 tggtcgccat gaagaaacgc aagacttgtg gatcaacgtt tttgatcatt tcatgtacag
     3001 tgatgaaatt ccctagagat ttactcattt tttcatcgtc ttcgccaatc gttacatagc
     3061 cattgtgcat ccagtaatta gcgaattttt tccctgtttt cgcttcactt tgtgcgattt
     3121 cattttcatg atgaggaaat tctaaatctt gcccgccacc atggatatcg atcgtttctc
     3181 ctaaatgttt tgttgccata acggaacatt caatatgcca accgggacgt cctttgcccc
     3241 atggtgaatc ccaagaaatc tcgccaggtt ttgcttgttt ccataaagcg aaatctaatg
     3301 ggtcttcttt ttgctgctgt tcttcccctg tacgctgact tgctccgact tccagttcat
     3361 cgatggattg gtcacttaat ttcccataat tttcaaattt acgggtacgg taatacacat
     3421 cacctgaaga ttcataagca tagccttttt caaccaacac ttgaataaat gctaaaatat
     3481 ctggcatatg atccatgact cttggatgca gtgtagcagg ttcgacattc aacgctttcg
     3541 tatcttcttc aaatgctcgg atgaaacgat cagcaacttc tggtgctgta atacctaatt
     3601 cgtttgctgt acggatgatt ttatcgtcta catccgtgaa attcgatacg aaatcgactt
     3661 tatagcctcg atattcaaaa tagcgtcgaa ttgtatcgaa agcaatggtg ctacgagcat
     3721 ttccgatatg gatataatta tacacagtcg gtccgcagac atacataccg acatgtccct
     3781 tttgtatagg gatgaattct tctttttttc tggtcaatgt attgtagatt tttatcattc
     3841 tgatctcctc actttttctc ctttgattcg aacgactttt gctggtactc ctaccgctgt
     3901 agcgtcgtct ggaacatctt tcaacacaac ggcacctgca ccgattttgg cgttttttcc
     3961 gatcgttacc ggtcctaata tctgcgcatt cgcagagagc atggctcctt gtttaacagt
     4021 tgggtgtctt ttacccgttt cttttcctgt gccgcctaat gttacgccat ggaataaaat
     4081 cacatcgtcc tcgatttctg cggtttcgcc aatgacgatt cccattccat ggtcgatgaa
     4141 cacgccagtt ccaatcgttg ctcctggatg gatctcaaca ccagttagga aacgccagaa
     4201 ttgagcattg atcttcgcca ataaaaagag ccggtgctga tataaaaaat gagagaaacg
     4261 atgccaaaac aaggcatgaa aaccagggta tgtcaaaacg atctcagctg tcgaacgtgc
     4321 agccggatcg tttctctttg ctgcatcaat cgctcgggta aaccaactca tgactttcca
     4381 ctcctttcgt aagaatttta tgtaggaaat catcttctta taaagaaaag aagcgccttt
     4441 taaataagtt tccttatcaa aagacgcttt ccgcgtggtt ccacttttct tcataatcaa
     4501 tgattatctc tgacagctaa cgcctgttat acgttcttgg ctactttgtt tcctataaaa
     4561 cagtttttcc tgttttctgt gtttcgccaa gaccactccc agaggcattt cggcaaccat
     4621 tcattgatac cttgcaccac ccggtatctc tcttgtaatg atcagctgct tacttttttc
     4681 tgttcatcgt gtttagttca gtacttgatt taaatgtgct aatgattttt cacgaccaag
     4741 caattcgatc gtttcaggca attctggacc atgcatttgt cctgatacag ctacacggat
     4801 tggcatgaaa agattttttc cttttacgcc agtttctttt tgaacagctt tgatggcagc
     4861 tttgacagtt ggagcatcta ctacttccat ttgttctagc tgtgctttga atgcgtggag
     4921 aacagtagga actgtttcac cagcaagtac ttcttttgct gcttcattca atacaggatg
     4981 ttcattgaaa aatagcgtag acagatcaac gatttctgca gcatagctca tttgaggttg
     5041 atacaggcta acgatcttct tcacccattc gattgtcttt tcatctgggt tttctgctac
     5101 acgtccatca gcgattagat aaggcagaca catgtcagta aatgcatcta gatccattgc
     5161 tttgatgtaa tggttgttta cccattgcag ttttttcgca tcgaaagctg cgggtgattt
     5221 acttaggcgt tttggatcga acattttgat caattcttgg tgttcgaaga tttcgtcttc
     5281 gcccacaggt gaccagccca ataatgcaat aaagttgaac attgcatctg gtagatagcc
     5341 taattcgcga tattgttcga taaattgcaa gattgattcg tcacgtttgc ttagtttttt
     5401 acctgtttca ctgttgatga tcaatgtcat atgtccgaag gttggtggtg tccagccaaa
     5461 tgcttcatag atcatcagtt gttttggtgt attggcaata tggtcatctc cacgtaaaac
     5521 gtgagtgatt ttcatcaaat gatcatcgac cgcaacagca aaattataag ttggcatacc
     5581 atcgcgtttt tggatgacaa agtcgccacc gatattatcg gattcgaacg aaatagcgcc
     5641 tttcaccata tctgtaaatg cgtattctgt attgcgaggc acacggaaac gtacaacaga
     5701 ttctagacct tgtgcttctt tttctgcttg ttcttcaggt gtcaagttcg cacatgttcc
     5761 tgcgtaatga ggcatttctc ctcttgcgcg ttgtgcttcc cgttctgctt ctaattcttc
     5821 ttctgtgcaa tagcatttgt atgctcgatt gctagcaagc agctgatcaa tcagcggttg
     5881 atagatttct ttacgttcag attgacggta agggccgtat tctccaggat tttcaggaga
     5941 ttcatcccaa ttcattccca accaagcaag gttttccaac tggctttttt cgccatcttc
     6001 gatattacgt ttttggtcag tatcctcaat acgaataata aagtctccat cattgtggcg
     6061 ggcaaacaaa taattgaata atgcagttct tgcatttcca atatgcaggt gcccagttgg
     6121 acttggtgca tagcgtacgc gtactttcgt cattttcttc ctcttctttc aaactagact
     6181 ttgactaaca gcaaaaattc cgtcagttac ggcttctcaa atatcattta tttaaagcca
     6241 ctcagcacaa attttacctt ttctaaggta aatagtaaag ccaactggtt gaatttcctt
     6301 accagttggc tgaatttctt ttttattttg attgtttagt ctttttctct gtctcttttt
     6361 tattgtcgat tcctcgtcct gcatgtgcag gtttagcaaa gatcatgcgt ccagcagctg
     6421 tttgaagggc acttgttaca acgacttcga tacgttcatt catgtaatgc tgtccatctt
     6481 ctacgacaac catcgtgccg tcatcaaggt aggctactcc ttgttggcgt tctgtcccgg
     6541 cttttactac tagaacattc atcgtttcac cagggatgac gactggtttt acggcatttg
     6601 ccaacgcatt gatattaaga acaggtacat tttggaattc agagacttta ttcaagttat
     6661 agtcattggt tacaatgaca ccatctaaaa gcttagccaa tttgatcaat ttgctgtcta
     6721 cttcactgat atcttcaaag tctccatcgt acatttcgac agatataccc tcttcttttt
     6781 gcaaggcatt taggatatct aatccgcgac gaccacgcac acgtttcaaa ctatcccctg
     6841 agtccgcgat gtattgtagt tcgtaaagaa caaaattagg gatcatcaag gttccttcta
     6901 agaatccagt ctttgtaata tcataaatcc gaccgtcaat gatcacgctt gtatccaaga
     6961 ttttatattt atggaaattt tctcctgatt ttcgttctaa cagttgactc tcaacttgac
     7021 tagctgtttc ttgctcgatt tttttgcttc gtgaaccaaa aactttcttc cattcttcga
     7081 ttcgtgtcgt tcccattctc aatcccagat agcctaagat gatcatcagt aaaatcggca
     7141 atacactatt gacgaatgga atttccatat tgtaaagagg ggtcgagatc acaacaccta
     7201 aagtcaatcc gatgatcgca ccgatcgcac cgaagagaag ataagtcaag ctgacctcac
     7261 ttagtttttg ttctacttgt ttgataatag ctgagatatg tttggccaac agcaaagata
     7321 ataaaaagaa aataagtgcg cctaacaggc tgttggtaaa tgtattattc agccatccgt
     7381 ttgactgttg accgattgcc tgccaagctg agggtaaaaa tgaaataccc aagcttgcgc
     7441 cgacaatgat catcagcaaa gtaatgacac gtttttgcat cgtttttcct ccgtttcgac
     7501 aatcatttat tagttagcgg aacacacgct tcaacgtttc agccaaagtt gctactccaa
     7561 ctatctcgat tccttttggc gcttcccagt tacctaagtt gtttttaggt aggtatactt
     7621 ttgtgaagcc gagtttttgg acttctttga ctcgttgttc aatatgactg acacgtctga
     7681 tttctccagt cagtccaatt tcaccgatga aacattccgt tggctgtgtt cctttttctt
     7741 tatagcttga agcaatactg acagcgatag ctaaatcaat cgctggttca tttaatttta
     7801 ccccacctgc agcttttaag taagcatctt gattctgaag aagcaaccca gctctttttt
     7861 ccagcactgc catgatcaga gatacccgat tgaaatcaag acctgttgtt gttcgtttgg
     7921 catttccgaa catagtaggt gtgactaatg cttggatctc taccaaaatt ggacgtgtcc
     7981 cttccattgc cacaacgata gcagagccag ttgcaccgtc taggcgttct tctaagaaaa
     8041 tctgtgaagg attagcgact tcttccaatc catgctcatg catttcaaaa atcccaatct
     8101 cattcgttga gccaaatcgg ttttttactg ctcgcaaaat ccgaaaacta tgatgttgtt
     8161 caccttcaaa atataacacc gtatccacca tatgttctaa cattcttggg ccggcgatcg
     8221 agccttcttt ggtaacatga ccgacgataa aaatggctat cccatttgtt ttagcggttt
     8281 tgagtagttc tgcagtggtt tctcttactt ggctgacact tcctgccaca cttgtgatgt
     8341 ctggctgcgt catcgtttgg atggaatcaa tgataacata atcaggggag atatgttcga
     8401 tggcacgact gatttcgttc atatctgttt cagcgtatag ataaaattct gtgttgatac
     8461 ttccgaggcg ttccgcgcgt aattttatct gctctgcgct ttcttcgccg gatacatata
     8521 agacttttcc gcctatggct gctaattgct gagaaacttg taacagcaaa gtggatttcc
     8581 cgatacctgg atcgccacca atcaagacca tagaccctgg caccacacct ccgcctaaca
     8641 cacggttcag ttcttctaat tttgttttga tccgaggttc ttttttaggg acgacgtctg
     8701 cgatttttgt tggttttgct ttttctcctg tcaaacttgt ccgagttctg cggtccgttg
     8761 tatcttgttc gatttcttcg accatcgtat tccattttcc gcaatttggg cagcgtccca
     8821 agaacttagg cgacacatac ccgcaatttt ggcaaacaaa ttgtactttt gatttttttg
     8881 ccattgtacc gcctcctttt atcctaatca tgagacatta aatcaagctt aagtatcttt
     8941 aacgttttat ttactggaag aaccaaatcc gccttgacgt tgagcagtcg tttgatcttg
     9001 atctgctttt aaaaacggca aaaagatccc ttggccgata cgctcgcctt ttttgatcac
     9061 tacatccttg aaaccaaagt tggtgaattg gaacatgata tggccttcat ttgcctcatt
     9121 attgtaataa tcactgtcaa tcacacctac tccgtttgtc aacactaaaa accgcttcaa
     9181 cggattgctt gaacggttag caagctgcaa aaattcatct tctcccatgt aagctttgat
     9241 tccagtaggc acgagggttg gtttcaattg tttttcattt tctgatacat cttttttcaa
     9301 tccatctgcg actgtttcca accccttttt ccagcctgct ttccagatac ttggaatcac
     9361 gacctcttct gctgcttcaa aatcatagcc agcagcatga tgtgtagcgc gctgcggtag
     9421 attgatattt tgatctttat atttagaaat aacttcaaat cctctttttc tcaccggttt
     9481 tcctccctaa ataaactact cctattctaa catatccgag gctgtaaaag actttcttta
     9541 caaattcttc aagaaattct gctatcttaa agtaaaccat taattcgttc tttttccaaa
     9601 cgactgcaag ttgtacaaaa aaactatttt ttacatgact ttactcgtca aaacaagtag
     9661 aatagaacga aattaatgag tggatatttg attgaggtgt cacatgcgta aagaaattaa
     9721 aaatgcaaaa cgaattattg tcaaagtagg aacaagtaca ttgatttatc ccaatggaaa
     9781 tatcaatctt gcggcaatcg atcagttagc ttttgtcctt tctgatctta gaaatcgggg
     9841 aaaggaagtg atcttagtct catcaggagc tgtaggcgtt ggtatgcata aattacattt
     9901 gtcagaacgt ccgactttga ttcctgaaca gcaagctgcg gcggctgttg gtcaagccga
     9961 actgatgaat atctataatc aaagattcag tgtatataat caacagacgg cacagatcct
    10021 cgtgacaaga gacgtggtag attatcctga aagtcgtaaa aatgtgagga atacattgga
    10081 acagcttttg attatggaca cgatccctat cgtgaacgaa aacgatactg tagcagtgga
    10141 tgaattagat catctgacaa aatttggcga taatgatcag ctgtcagcca tcatggcaaa
    10201 acttgtcgcg gcagaccttt tgatcgtcct ttctgatatc gacgggtttt attcagataa
    10261 tccgactaca aatcccgagg ctcagatgta tcgctatatc gataaaatcg atgaaaagat
    10321 tctggcacaa gcaggtggtg ctggtagcac atttggaaca ggcggtatgc agagtaaatt
    10381 aaaagccgct tctaggatat ttgaagcaaa cagcgcaatg attttagcaa atggaaaaaa
    10441 tccggcaatt atttttgata ttcttgcagg aaaagaagta ggtactttat ttaaggagga
    10501 cttgaaatga ctgacttgat gcaattagga caacaggcaa aagatagcgc gcaacaacta
    10561 gcacttatgc ctacaaaccg caaaaatgag ctacttgttc aaatggcaca aaccatcaaa
    10621 aagaatcaac aagcgatcat tgaagcaaat cataaagact tagaaaaagc agtcgaaaac
    10681 aatatcagtg aaacgatgca agaccgcttg cagctgacac ctgagcgaat caatgccatg
    10741 gctacagaga tcgaaaaaat tgcttcgtta gacgatccta ttggaaaagt agatgagatg
    10801 tggacgaaca ccgacggatt gagaatcggc aaaaagcgcg tgccactagg cgtgatcggc
    10861 atcatttacg aatctcgtcc aaatgtcacg acagatgcag ctagtctagc attcaaatca
    10921 gggaatgccg ttatccttcg cggtggcaaa gaagcattct tctcgaatca attattagtc
    10981 caattattgc agcaagtact ggtcagtgct ggcgaatctc cttatgccat ccagtttgtc
    11041 gatgatactt ctcatgaaac tgcaggacag ttgatgcgat tgacagaata cttagatgtg
    11101 ctgattcctc gaggcggagc aaaattaatc caacgagtca aagaacaggc aacggtacct
    11161 atcattgaaa caggtacagg aaataatcac gtatatatcg ataaagaagc acagttatct
    11221 atggcagtga acatcatcgt caatgcaaaa gcttcccgtc catctgtctg taacgcggca
    11281 gaaacattgc tgatccattc tgagatcgca ccttttttct tgccagctat tgaaaaagaa
    11341 ctggtagaac acggggtctc tttacgtgcg gatgctcgtg cgttagaata tctagagacg
    11401 gctgctttag cagaagacac agattgggat actgaatatc tagattatat ccttgctgtc
    11461 aaagtggtcg attctttgga tgaagcaatt gcacatatca atcggcataa tactaaacat
    11521 tcagaaacga tcgtgacaga tagttatgcg gctagtcaac gtttcttaaa tgaagtggac
    11581 gcggcggctg tctatgtcaa tgcctccact cgttttacag acggatcggt atttggtttt
    11641 ggtgcagaga ttggtatctc tactcaaaag ttgcatgccc gtggtccaat ggggttaaat
    11701 gaattaactt ccacaaaata tatcatttat ggcgatggac aaatccgcga ataatgtttc
    11761 gtgtgaaaca ttattcccgc cttttgcctt ttttctgtaa aggttctatc caactagaaa
    11821 ggtgtcaata tgtttgaaat tttaatgatt ttttttattt accttatttc tctgaatatc
    11881 gctgcttttc tgggagtcgg tattttatcc cttttctttc aattcaaaaa acgaagcata
    11941 gggagtcaac gggaaaaatg gtcgcagtat tttgacaaaa tcgggcaaaa agggctggtt
    12001 acacgcttgc acattagtta catggttgca ttatgcttat tagcgacaat caattactat
    12061 tcttttttcg atcattcaat cgcttatacg attacgctac tgattgctgg gatttttcac
    12121 ttatcttata aatatcagct gaataaaaac cacttgaatc ggactttccg ctaaaaaata
    12181 tccgtacgat aagtagagtg ccttgaagat cggcaaaaaa ctgccggact ttttagaggc
    12241 tgtgacaaag attttgtcac agcctctttt tccatataaa cggcgttcgc ctaccagctg
    12301 cctggtaaat ctgtttcctc ttcgttaaga agcgaaaatc cgtcaaatgt ttcgccatca
    12361 taataacgaa gcagctcatc catggacaag ttcatcgttt tttctttttc ttcttccgct
    12421 gattcagcca gtagctgttc ccgatacacc tgatacatac cagctatttt ttgctgcatc
    12481 gcttttctag tttcaccttc cgcattgatt attggcaatt ctttgatata gccacgataa
    12541 atcaaccgat catctgaaaa ttcaatgggt aattcaacga gtgtcaaccc gacttcctga
    12601 aaatattctt ccagcatcta ttatcccccc attctttttc ttaaatgaat tttagcttac
    12661 ctactgaaaa ttttccagtt tcattatttt gagaaaaaaa gggaatgtga caaaaatcgt
    12721 gtcacatccc ctttttccga ataaatggtg ttcttctgcc aactcctcaa tcataagtca
    12781 caatattcaa caaacatgag aaactgtttt ttgaaattgt tccttgtgat tcagagttaa
    12841 acgacagtct cacaacctct tggtaaacgg cgttcaatca gctgaccctc ggtattagct
    12901 caaaaaacgg taaaatatga gaagctattt ttccgttttg tttctctaat acttaggata
    12961 aaacgctgat tttacaacct ctgttaaacg gtgttcaatc agctgtcctt cggtattagc
    13021 tcaaaaaacg gcaaaatatg aggaactatt tttccgtttg tttctctaat actcaggaca
    13081 aaacgctgat tttacaacct cctgataaac ggtgttcaaa agcttgcatc tgcggtaata
    13141 agcccaacca aacgaaaaat atgagaagct atttttgttt ggctgttctt atgacttgat
    13201 gcaggacagc tttttcacaa cctctgattt cttattcttc ttcatttgta gcaaaataat
    13261 tacggtgata aatgatacaa catacactga taagaacaat ggcacctgca aaataaaacg
    13321 catagctttc accaatatgt tcaccaatca cacctgcaat aaatggtgct aaagctccac
    13381 cgaaaaagcg gacaaaacta tagctggacg aagcaacgtt tcgttgcaga cctggtattt
    13441 ccatagcaat cgtcgtcaac agtgtattga taattccttg gaaaaatcct gctaacacaa
    13501 tgctgatcgg tacaaggact ggcatagaag ttccagctcc taataacagc aaacaaataa
    13561 tgaataagac aaatgctgct aaaattgtcc gataagtcgt aaacttccgc tcaagagccg
    13621 gtgcaacgaa aatcgaagca atagccagaa gcacacccca gccaaaaaat acaaaaccga
    13681 cttctaattc agaatagcca atcaataaaa atggcgcata cgcaagtaaa gtaaagaatc
    13741 cgaaattata taacaatgcg attagtccaa ctgaacgaag tttatgattg cttaacgctt
    13801 tgacaccttc ccaaaggcct acttttctct ttggtttctc agtttcaggt aaaagtaccg
    13861 ctagggctat aaaaccgcaa aacatcaaaa atgctacccc aaaaaatgga taacgccatg
    13921 aaccactgcc caagacccca ccgactagag ggccaacagc cattccgcag ccaagtgctg
    13981 cttcatacat catgattgct ttttctgtgt taccaactaa aatactgacg atcgttgcta
    14041 aagcagtaga gacaaataaa gcattcccga caccccagcc ggcacgtaac ccaatcagca
    14101 ttccgatatt cccagataat ccgccaagac cagcgaatac aacgatgata aacaatcctg
    14161 tcatcaatgt ttttttcaaa ccgatccgac tagataagaa acctgtaaat agcatcacca
    14221 caccagtgac taacatgtaa ctagtaaata acagggtcgt ttcagctggc gtagcgttta
    14281 actgtgcagc aatcgttttt aagattgggt ctactagacc cactcccata aaagcgatca
    14341 tacaggtagc tgctacaact agcgctgaac ttttcccctt tgtttcttca tttgtttgtt
    14401 cttgtatatc tatttgattc tccaatattt ccatcccttt ctctctatta gcatcataca
    14461 tgatttattt tacatgtcaa tatgcacatg taaaacgtgg aaactttact gtgatataat
    14521 aaagaaaata agacgagagg taggattttt atgaatactt tagcctatat attattatgc
    14581 gtgcttttat ccaaaccacg ttctgggtat gaattgaagc agctgatcac aattttttgg
    14641 gaagcccatc acagtcagat ctatacaacg cttgccaagt tagaccagca gggttatgtg
    14701 accgttttgg acaaagacga gcactctcaa aaaaagatct accacttgac tgatgaagga
    14761 cgtgctcttg tagaggaatg gatcaaagag gaaactcccg ctcctacgca aaaagatgaa
    14821 tttctagcaa aaatctatgc tttttcgacg ctcgataaaa caacagctac tggactgcta
    14881 ttcactagag aacaacagct gaacaaaatc atgcaaaaaa atcaaaaaaa attgaatgac
    14941 ctctcgctag acaagaaaca agaatttgga cgttatgttg tgatccagcg cagaattatg
    15001 ctttgtcagc aggaactcca atggtgtcag caagtaagag aacaaatgca gcagtttttt
    15061 gagtaaggga taaacagaac ctttctagaa atatctattg aagagcaaat tatcttcaat
    15121 aaaagagcaa tacagttgga aaagaaaaag cgatccgaac gacaggccaa aacctatcgt
    15181 tcggatcact atttttctag aactgcattc aagagactat gacaaaaagc atgccaaggc
    15241 cttttttcaa ataacagtgt tccatcattc attactttga attgctttta gcacacttct
    15301 tgaaagcggg ccaacgatga tcaactgcaa cggtaaagcg gcaatcacat tcattttcca
    15361 agcattcata taagcagaga ataattgatc aggtattcct tgttccatga tcacaccgaa
    15421 taaagacata catgtcacca ttcctaaaac catcaagcat gaaatagtta agatcatttg
    15481 gaccttgctt tctggattta ctggtagttt aaaggcaatt tttttcgcca gcacaccgat
    15541 gatgaagaca tccaaaatga aagccaccac gaatccaggc actagaccaa tcaacaaact
    15601 tgataaatga agatcttgat gcagccataa attatacgcg ctcatcccca agaccatcaa
    15661 aaaacacatc attgttgtaa aaattattcc ttctttttta tttgtaggca atctgtacca
    15721 ctcctttaaa tttgtcacga aaatcatcgt agcacattta aaaatttctc gtaagtactt
    15781 tttttatttt ctgccctttt atttcataaa cttcatcagc cgtacgctcg acaaagtaag
    15841 aatcatgaga agtaaacaaa atcgttcccg gatatatttg cattagcttc tctaaagctt
    15901 cgatcgttgc catatcaata aaattagtcg gttcatctaa cagcaacaca ttacttggct
    15961 tagtaaaaag caaagcaatc gtcagacggg tcgcttctcc cccacttaat gtacataacg
    16021 gtttcgtcaa agtttcctga gcaaacccta agtgattcag caaactacgg acaagcgatt
    16081 ctgaaaactc tgtttgtctc atgacaaaac gaatgatgga ttcttcagat gtcatttgat
    16141 aacccttttg ctgatatact tgaaagctta ctttcggaga gagaaggatt ccttctcctt
    16201 gtttgcggat atgttctaat aaggttgtct tacctgaacc attttcaccg acaatcgcga
    16261 tttttttacc taaagaaaac tgaaaatcac catttaccag taatgttctc ccacttcttt
    16321 ccaattggac attttgtccc atgatgggat agcggctgtg gagttcaaga gattttggta
    16381 ctggaaaacg gatttgtttc atctgctgcg gcttggttgt ttctcctatt ttttggagtc
    16441 ttctctctaa tgttttcgca ttcttttgga tggctttttg tacactgtct ttttgtttag
    16501 aggaagacaa ccgatccagt ctgatttgtt gctttttctt tttactggac actttttcca
    16561 acttttccgc ttgttttttc ttttcttgga tcgatttttc caatcgtttt ttctctttca
    16621 tgatcgactc tgcttctcgt agctgagtct ttttctccaa ttccttttga gtgagatagg
    16681 cactataatt ccccgaaaac tctcggattt ctccgtcttt tacttcccag attttcgatg
    16741 ctagctcatc tagaaaatag cgatcatgac taacaaaaac cagcgtacca taataataac
    16801 gaagctcttc aatcagtcga tcaatacttt ttctatctag atgggttgtt ggctcgtcta
    16861 gaagcatccc ttgttcataa gaagaaagaa gacgtatcaa ctcgatcttt cgttcttccc
    16921 ctccgctcca tttccgctct gaagtttctt ccagtcttaa ctggaaatga ctggctaatt
    16981 ccaaatttag gtcctttgct tcagcgagat aggttaattg aggcaggtag ttgaaggtga
    17041 tctctgtttg gattttccca tgatcgggaa aaagttcacc ggctattagt ttgagcaaag
    17101 tcgatttacc gacaccattt tctcctatga tccctatacg atcttgctga tagacacgaa
    17161 gagaatcaat agatagttct agctggttgc caaagctgac ttgtagattt gttatattta
    17221 ctgctaaatt ttccatatgt tttacactcc tatttcatgt aaaacaacaa ttatcgatac
    17281 taaaaaagag cagaaaaatc tgtttgcaaa aaaactgcat cacatctttt cctactctga
    17341 acgaaataac tatgactaaa tcaccaaatc tgtcatagct ttcacctatt ctactgtcgt
    17401 tcgaaaaaat ggacatactc catccatgaa attgttgttt atacatgtct tttgaattta
    17461 tttagttgtt attcaaaagt tcgaaacgta atttcatcga tgcagtcatt tttccgacct
    17521 ccttttttat taggttacca cacacgattc aaaaaaagca atagctactg aatagtaact
    17581 gaaaaccttt tattcgaaca tagtgatctt ttctggtgct aaacgaacag actcgtaacc
    17641 ttcttctacc gcttttccta ttgataaaat aataactgga acataacggt ctttatccaa
    17701 accaaatgcc tctgccaact gatctgcttc gaatccacca attggatttg tttcatagcc
    17761 atgatcacgc gcaactaaca tgaattgcat agccgctaag ctagaatcga ctttgactac
    17821 atcgttcatt tcttctcttg aaaaactctt gtaataagga atgatcgcag ctaattgttg
    17881 atctctgact tcttttggca tcttccctga ttcgtaagct tggttgtaga tttcttcgcc
    17941 atattcgtaa cattgcatat cgccaaaaat catgaccatt gcagaagaag tatcattttg
    18001 tctcgtattg aaacggatca aaggacgcaa gatttctttt gcttcctcgc tttcagcgac
    18061 cacaaagcgc catggtttca tattgacaga agaaggtgca gtcgtcgctt ctttgatcat
    18121 ttccagcatt tcttcgtgtg ggattttgta tgtttcatca tatacgcgaa cggatcttct
    18181 accagtcata attgttgaaa aatcattttt ttgttccatt attattaacc tctttcgttt
    18241 ctgttgttat attgcaaagt tgggtattca atttatctaa aagtgttaga aattgtttca
    18301 tttctacatc gctgaaagcc ggggatagca cggaacctgt atctttatgg tccatttcac
    18361 aatgcttgat tttcttctgg gctttttctg ttagcgctac atacacttcc cggttgttct
    18421 gaggatttct ctgacgcgaa acatagccat ttgcttccaa taccttcaga tgacgagtaa
    18481 ctgctccttg atcaatctgc aacttttctt gcaaactgct ttgtagcaat ggattttctt
    18541 ctttcagtct catcatgatc tcgtatctag taagacttat accgatattt tcttcaaaga
    18601 tctggacgat tttttgatcg gctaatttga tttgatataa aaggtgacta atctcttcta
    18661 aagacataat ttcctccttt ctaataattg actcgtcaac gattgacttg tcaataatag
    18721 aataaaaaaa gaaaaaagtc aaagaatcag ctgtgtaata aaaaaaacgc tctcaataga
    18781 aaagtataga gaaaagaaat tttttgtaga atgaacggaa aaaatgataa agaaagcaca
    18841 acgctaaaac caagattatt gattcttatc aaactataga agatagctct tctgcagtag
    18901 ataattttac accgccaaaa atttccgaaa tcgccttttg caagaatccc gtcgaatatt
    18961 agaaattgga tcagtatgct taaacagtaa taaaaaagtt atgacaagat gttgtcacaa
    19021 ccttttttga gtaaacggtg ttcaaaaact tgcatctgcg gtaataagtt caaccaaaca
    19081 aaaaacatga aaatctgttt ttgtttggct gttcttacta cttgatgcag ggcagctttt
    19141 tcactacctc tttttaataa ccataaaatc ctattccttc atatctccag cccatcggtg
    19201 ttagccaatc tctttcctta gcactcaagg tatagtgatg ggtaccagtt ttttcatttg
    19261 gattaaatag tcgataaatt ggcttggaac caccagaata aaaacttaga ccttcataac
    19321 gccaaccgat ttttgttaaa gagttgatct cagattgtgc cattgtgtaa tgatggtctc
    19381 cactgtaagg attgtataaa cggtagacag gttgtccgct gtttggtgct tgccaagcaa
    19441 tcccttcata ttcccagcct acagttggca gataattttt ttctccggca ttcaatgtat
    19501 ataaatgttc tcctgtatga cggttataca gacgatacat cggagaagtt gaagttggtg
    19561 ctgtatcata ctgtgtcaga ttataggttt cgattacatt gttcaatgta gaaccataat
    19621 taggcgcagt ggcataacga cctgttagcc aagccgtagc atctttgtaa gagttcgtat
    19681 tgcttttcca tgcaccggaa tagtagtaaa cgcctggctg aaaagaagtc gtcttcaata
    19741 ccgttacgtt atcttgcaat gattctttgt acgaaggata ttttctaaaa gcggcattca
    19801 ccgtaatcca ttgcccattc acatattccg aagtaggcat cgtaacagat tggccattat
    19861 agcttccttt aatcccaaat agattgtaat taggggcaga agccaaagta ctgttccccc
    19921 agccactttc aacgatggct tgagcaatca tgactgatgc atataagtca ttggcacttg
    19981 ccaaactttg tgcagttgag gcaatactat caatgaacgc ttgttgagaa ttagcagatg
    20041 agcgagcata aaaagcatag ccagtatagt tttcgaattc agacaaatct gcttgatggg
    20101 tatgtgtcaa tgtttgccca tctgtcaatt ggttcgtgat ttcttctgca ttctctggat
    20161 tgttctgagc ttctgctgat gtactgtctt cggtttgtgt ttgttctgtt gtttcagttg
    20221 ttggttcttc ggttgaaatt gtattttctg agacagtagc tgactgcaaa gtactttctg
    20281 tatcgttttc aattcctgtt tctgaagaac tcgatgtctg agttgcgaca gtttctgtcg
    20341 attcttcagc tagcactgtt gtgttggcaa ctgctagatt tcctaaaaga ataacgctaa
    20401 ataataaatt ctttgctttc atcttttttc tcctttgaat tatgaaactt ttcctaaata
    20461 acagtgtaac taaaaaagta aaagcaagtc aatgtccata tttaaataac atttgtttct
    20521 ttttaataaa tttgcttttt ctgcataaaa agacgtaaat gaagaatatt cttctccatt
    20581 cacgccttaa attgatggtc gtttgaataa ctatttaaca agtaattctc tctggatatt
    20641 atccgttatg gattatactt cctgtgtcgt cggacggata atcatgttgc tgacagacat
    20701 ccggtctggc gtttctatcg caaatgctac agcagaagct atatcttcag atgttaatcc
    20761 ccattctttt tgcgcttcat gaagttcaag agcagccttt ttgtcgctga tggtttgata
    20821 taattcagtt tgaacagctc ctggagagat gatcgtagat ttgatgttat tttgccgttg
    20881 ctcttgacgt aatccttcca tgattgcacg gacagcaaat tttgttccgc aataaacggc
    20941 ggaatcagga taaacaacat gtcccgccac agaatcagtt gaaatgatat gcccagattt
    21001 ttgtttttcc atgattggaa gtacggcgga aattccattt aacacaccca tgatgttgat
    21061 atcaagcata ttttgccatt catcacggtg accttcaact aatggagcgg ttggcatgat
    21121 tcctgcatta ttgtacaata catcgattcg accgaacttt tccatcgcta aatcgataac
    21181 tgcttggact tgtgcaaaat ctcttacatc tgctgtttga atatatagct cagcttctgg
    21241 taaagattct tttattgctt ttaggcgatc ttctcggcgt gcagctataa ctagtttagc
    21301 ccctttttct gctaatagct tcgttgttgc ttcaccgatt ccgcttgaag cgcccatgat
    21361 gacaatcact ttttctgata aatcattcat tctattctcc tcctgttttg aaaaatctct
    21421 tgcttatact cactatctct tatttcatct tactgttcaa ataatccgtt cgacaggttt
    21481 gacgattttg gcagggattc ctgcaacgat ggtattgtct gggacatctt tcgtaacagt
    21541 tgcattcgct gcaacaatgg cattctcgcc aatcgttacg cctggcagaa tagttgctcc
    21601 tgctccgatc caagcatttt tcttgatgtg gattggcttt actgacaagc ctcttcgttc
    21661 ttttggatcg gttagatggt tcaccgtgat gaggttgaca cgaggaccaa tcaatactga
    21721 atcttctaaa cagatgccac ctaagtcaac gaacatcact tgacgattga taaagacatt
    21781 tttccctata ctaatatgtt tgccgaaatc tgtataaaaa ggcagcagaa cagtcaccgt
    21841 atcatctact ttgtcactaa tgatattttc tagccgtttg cggatatctg ctggttcatg
    21901 gtacccagta ttcagcgctg ctaaaggctc ttcgttttcc gcttggatct gatggatcac
    21961 ttcaaagagc ggatccgtta ctcgaatctc tttattgacg atttgttttt cgatgaggct
    22021 tgtcattttg ctccctcctt ttatccttac tttataacgg acggcttatg atgtctaata
    22081 cttataaaac atacttaact attcctttta actatactta gtggtatgct tttttaaaaa
    22141 caggagggaa aaaatggata ttcgtatact gacgtatttt ttagcagtag ctagagaaaa
    22201 aaatatcagc cgagcagcaa aatctctgca tatcacgcaa cctactctct caaaacaact
    22261 gaaagattta gaagaagaac ttggtgtgac acttttcact cgaggctccc gagagataca
    22321 gttgacggat tctggtcgat atctttattc aaaaggaaaa gaaatcttgt cgttggtaga
    22381 aaaaacggcg aacaatttaa caaatgacgc aatcatctct ggagaaatct acattggcgg
    22441 aggcgaaact aaagcaatct ctttcatttc tgatagcctt cattcgttga tcaaggcgca
    22501 tcctgatatc cagtttcatt tatatagcgg gaatgcagat gatatcatag acaaactgga
    22561 tagcggatta cttgattttg gactcgtaat cgaaccgaca aacaaacaaa aataccagtc
    22621 atttcgactg ccagcccaag atcgttgggg acttttgata catcggaatc atcctttagc
    22681 aaagcaaaca gtcatccatc cgcaggaact gaaaactgtt cctttgatca tttcccggca
    22741 aacgtcagtg gacagtcaac tagctgaatg gctaggtttc agcatagatc agttatcgat
    22801 cgtcggaaca tataacttat tgtacaatgc ttctttgatg gtcgataacg ggacttttgg
    22861 tgcattagct ctagatggga tcatcaatac ttatgggacg gaaaaagtat tcatcccact
    22921 cgatccgcca ttacaagctt cactgaatct catttggaaa aaagaccagc ctctttctcc
    22981 tgctgctcgt aaatttttag atgtcctaac ggagaaatac agttaagtag agtttttttc
    23041 cttcgctttc agctttttaa caacgaataa gttaagcaaa agatccaatg attaaataac
    23101 ataagtttct taatttgttc cttcctttta tttctgataa ttccagtaaa ttactataat
    23161 tataaaaaaa gaattgacaa aacataactg tctatataaa attgattacg ttaacaataa
    23221 ttcgtttcat gtgactagat catactaggc atgaaaaatc tctgaggaac atggtttccc
    23281 taggaggttt ttcgtgtcta tttttgtttg gccgcaatca actggtctag caaacgtgaa
    23341 aaccaaatct tcaaaaaggg tgcgcgcatg aaaatcaaaa agatctttaa tcaaaatgca
    23401 gtattggtag atgacggtgg acaggaaaaa gtagctattg gaaaagggat tggatttgac
    23461 agaaaaagaa atgatttgat ttttgatcgt gatgtcgaac ggatatttgt catggaacca
    23521 gaaggtcaaa tcaagttgca aaatttactt aatcagattg atgaacgctt tttatttgca
    23581 gcagaaacga tcattgatca tgcggaaact gttttgatgg aaaagttgaa tgaacatgta
    23641 ttgatcgctt tgaccgacca cattgctttt tctgcggaga acattaataa cggtatcgtc
    23701 atccgcaaca aacttttaag agaaatcgaa gtgctttaca gtgatgaatt cagtattgct
    23761 caatgggcag tagagtatct gacaaaagag cttgggattc catatactta tgacgaagca
    23821 ggatatatcg caatccatat tcacagcgga cgaagcggtc ggaacaataa ccatcgcagt
    23881 atccgtgaag tgacgattgt atctgacatc atccatttag tggaatcaga attggaaaca
    23941 gatatccatt cagaaccatt ttcactgaat tattcaagac tggtgaatca tttacgactg
    24001 cttctacaac gaacccatgc gcaacaatat gctgttttag acacggaaat cgtagaaatg
    24061 gtcaaacgaa aatacccaga aagctataag atttctaaaa aaatccgtgt gttgctgacc
    24121 aaagaatacc agctcgcgat cacgaaagaa gaactgggtt acttggccat ccatatcgag
    24181 cgtttaagaa gcgcaatcgt acaaacaaat gttaacaatc atgaggagga aaaaaattga
    24241 aagcatatct acaacgaatg ggacgttccc ttcaacttcc agttgcggtc ttacctgctg
    24301 ctgctttact agtagggatc ggaaactggt gggcatctta tagtaacgac attgtcgctc
    24361 attttttaca agctggcggt aacgcagttt taggtcaatt acctttgctg tttgccgttg
    24421 ggttagcact agggatgtca gtggacaaat caggtgctgc tgccttggca ggtttggtcg
    24481 catttgaagt tccagtaaat gttttaaaaa ctgattcagt agctacttta ttgaatatca
    24541 aaccagaatc agttgatcca tcttttgctc agatcagtaa cgtatttatt ggtattattg
    24601 ccggattgat tgcggcagct ctttataatc gtttccacga agcaaaatta ccgatggctt
    24661 tatctttctt tagcggcaag cgtctagtgc caatcttggc tgcctttgtc atgttgctga
    24721 tcagtgcagt gctactgctc gtatggcctc cggtttactc tgctttagtt tcttttgcta
    24781 aattcatttt gagtttaggc tggggtggtg caggtcttta tggcttcttc aaccgtttac
    24841 tgatccctac aggattgcat caagcaatga actccgtgtt ctggtttgac gtagcaggaa
    24901 tcaatgatat tggtaaattt ttggcttctg aaggaacaaa aggcgtaaca ggaatgtatc
    24961 aagctggatt tttcccaatc atgatgttcg ggcttccagc aggagcatat gctatttaca
    25021 gaaatgcacg tccagaaaac aaagcaaaaa cagcttcctt gatgttagca gcagcttttg
    25081 cttcattctt tactggtgta acagaaccac ttgaattctc atttatgttc gttgcttggc
    25141 cattatatgt cttgcatgca attttcactg ggatttcttt agcagtgagt gcattcttcc
    25201 attggacagc aggatttgcc tttagtgccg gatttgtcga ttacttccta tctttgaaaa
    25261 atccgattgc gaatcagcca ttgatgttga tcgtccaagg attagtaaca gcagtgattt
    25321 actactttgg ctttaacttt gctatcaaaa aattccatct gatgactcct ggccgcgagg
    25381 aagcagatct ttcagatgac gatactgcaa cgactaattc agacaataaa tatgcggcac
    25441 aagcaagcaa aatctacgct gcattaggcg gagccgacaa tgtaacttcg atcgataact
    25501 gtactacacg ccttcgtttg caagtcaaag atacaggcct gatcgatcaa aataaaatca
    25561 aagccactgg cgtacctggt atgaagatca tcgacggaaa aaatgcgcaa gtcatcgtag
    25621 gtacagaagt acaattcgtt gcagatgaga tggcaaaact acatggtggt gctgcaacac
    25681 gacctgcaca aacaaacacc gtggttaaaa cagaaacatt tgccggtgga acgatcgaac
    25741 aagatattta cgctatcgca aatgggaaac tgattccaat cactgaagtt tcagatgatg
    25801 tattcgcgga aaaaatgatg ggtgacggct atgctgtatt accagaaaat ggagaaatct
    25861 tctctccaat cgctggtacg atcacaaaca ttttcccaac gaaacatgct gtcggaatcc
    25921 aaacagatgc tggtatcgaa gtcttgcttc atatgggcat caacactgtt gacttaaaag
    25981 gcgaaccatt cactttatat gtaaaagaag gtcaaaaagt cgcaagaggt caattgattg
    26041 ctttagttga tcttgccgct attcaatctg caggcaaaaa cacggatatg gtcgttgtat
    26101 tcacaaatgg cgacaaagtc caaagcttag aaatcgaacc cgcacgtgat gtaaaagcaa
    26161 atgataaaat cggctctgtt tccaacaaag cctgagccat cgtcttcccc agactttttt
    26221 atcgataaac aaacaggagc tgagacaaaa atcttgtctc agctcctttt tccgaataaa
    26281 aacggcgttc tcctgccaac tccgcggtaa taagtccagc caaacgtaac atatgaagag
    26341 ctatttttta tttgactctt cttattactt gatgcagaac agctttttta caacctctga
    26401 taaacggtgt tcaaaaacca gtttatcggt atgaagtcaa atttatgcaa aaatgtggaa
    26461 agccattttc acaaaatttt tcttcatact caaaactaat cggctttttc acaacctctt
    26521 tatacattat tcctgatcca atatttcttt taatgctgct tcaagagaag ttaatggttt
    26581 tcccaatact ttttctaaat catcagagga aatagctaac tgatcctttt taatatcatg
    26641 ttggatcgct aaaaacatgt cagctgcttc ttgagggaat ccggcttctt ttagattttc
    26701 gacaaacgct gcatcacttg cttcaataat ttctaacttt ttacctgtcg cacgctctaa
    26761 tgccttggct aattcttcat agtcagttag attgccggat aattctaaga tttctggaga
    26821 gtcactttct actagtactt ttgctgcagc ttctgcatat tcacgtttca atgcccaacc
    26881 tgtttttcca tttcctgcag cataaacgaa ttgaccagtt tttaaagcct gcccaatgat
    26941 aggtaattca ttctctacat accaattatt ccgtaaaaat gtatgggaaa tacctgaatc
    27001 cttaatcatt ttttcagtga attggtgatc tggcgctaat acactgatcg agtgatcggc
    27061 atctgcaaaa cttgtataag cgatataaga taccccagct gcttgtgctg ccttcactac
    27121 attcttatgt tcggcttgac gatttcctgg aacgccagag acaaaaagca atcgatctat
    27181 tcctttcaac gcttctttca ttgagtctaa atcatcataa tcaccaatac gtatattaaa
    27241 tccagcttct ttcagtgctt ctcctttttc ttcactacgt actagtacat agatttcatt
    27301 ttgtggaacc atctttttta agaattccag cgcataacca ccaaaacctc cagttgctcc
    27361 tgttactaaa taatgcatat ttgccactcc ctattctttt tatttgttaa gatttacagc
    27421 tgtacattta taaattacac ctttatcata aagagtgtgg taagataagt caagcaaaaa
    27481 aaatgaaagg atgacaactg atgaagtatt ctctgcaatt tagtgatgca atccatgtac
    27541 ttgcctatat tgaaatgttt caagataccc gatcactttc tagtgagatg attgccagta
    27601 gtatcgaaac aaatgcagca aatgtacgta aaatcatgag tcagctcaaa aaatccggtt
    27661 tgatctttac aaaaaccgga aaaccgcagc cttcccttgc caaaagtcca gatcagattt
    27721 ctttactaga tgtctatcgc agcatcgaag gtaatacaca gcttatccaa gtcgatccaa
    27781 aaacaaatcc aaattgcgtc gttggtgcga atatccaaga agctttagaa gaaagctacg
    27841 ctcggctgca gaaaaaggca gaagaagaaa tggctaagat cacacttgat agtctgatac
    27901 ataaaatagc cgttttggaa aaagaaaaaa gaccagaaaa cacaggcctc gtagagagat
    27961 ttttataatt ctctctactg tgcctgcatt tttctgatcg gaatcgtcta cataaattct
    28021 tttattctcc gggggacaaa agccttgtca tgcccctttt tcctctctaa tgctcaggtc
    28081 aaagcgctga agtcatgacg tcttgataaa cggtgttcaa gtagctgacc ctcggcatta
    28141 gttctgaaaa atgcaaaata tggaaagcta tttcccattt ttcctctcta atgctcaggt
    28201 caaagcgcta ctttcacgac ctctatatta tcatggttta ttgactaccg gtatccagat
    28261 ttctcccata tatttcccgt tggtaactcc catttcgact acagcatttg gacccccaac
    28321 ataggcgtaa tcttctactt ctcttaaaac atgcccaaag gccgtattcg tcaaatcatt
    28381 tcttaattct tcaggcgatt ctgctaaagc gattacaaca agatacgttc cttttgggaa
    28441 tgggatgatt cgggtagtgt ttggaacatc ctgactcgtt tgaacaccga catagtacat
    28501 cattttcccg ttatagggtt cgttgactgt aaagaaataa tgattttcag cagcttgttt
    28561 caatgcatct attgttccat taccgataac tttttgctca aatgcttgtt tctctgcagc
    28621 caaaccgaca aaatccgtat tgctactctt caattccaca ccaatacccg tcaccgtaaa
    28681 atctggtttt tcttcgatcc tatactttgc catttctccc accctttcta ttatattaag
    28741 ttcatcttac ataaaattaa agggaaatga cacgattacg ctcttttttc tagggatatt
    28801 tttatttttt aactattttt ttgctttcac tgcaatccaa atctcactgt aaacattact
    28861 gaaatcctct ccccattttg taaaagagat ctcgggagct tcgactaact cataatctga
    28921 agaaggaagc cattctgcat agattttccc ccaagtatct tgtaaagttt ggggaaatgg
    28981 ccctttatta ggaaaaatgg cccaagtgtg ttcttctata gcaagcattt ctaaatcagc
    29041 gtacggattt tcttttgtcg tagcaaagcc aatcatgtgc atcaaactgc ccttttcttc
    29101 caagcgggct ccatcaaatc cataagaaac gttgattact tgatttggat ataaatctcc
    29161 taacttatgc atctcttccc tttgagtctc cgtgatcgtc tgcgctaact tttgtatttc
    29221 ttgattgact ccttcaaatt tgacaggaac ttttttcgcc acacctgcca cataaaactg
    29281 ttctttcttt tcgatacgaa attccatcga ttttcctcct ctgacatcaa taaaaaatgt
    29341 caagcgtgga aaagattttt gtatgccgta tctgctgatt tctgaaggca gatatcctga
    29401 ccaatcacga aaagctcttg aaaaaccttc gactgtttca tatccatatc taaacgccgt
    29461 ttctgtcact cttgatccat ctaggagatc gcgattagcc aaagccaatc tacggttttt
    29521 gatatagtcc attagcgaca tccctgcaat aaacgtaaag atgcgtttta attgatattc
    29581 agagattcct gtattttcgg ctattatttt ataggaaagt ccttctgtca aatgttcttc
    29641 gatgtaatcg atcaattggt tcagatcttt aagcatattt atcttcctcc ttgcttgcct
    29701 ttttatcata ttatcaaaga aagtcaatta cttgatgaat caggaaccaa tttagtcaga
    29761 tgtagaaaat gttgtatgga tctaaagaaa attctcaagc agcgtttcgt aacggataat
    29821 tggtattatc tgcctatcta tgttttacct ttagaaaaat aaattttggg gaagacacat
    29881 tctaagtaaa caaagcggta aaaatatctt ttattccctt agttaatccg cttactacct
    29941 actgtacaaa gcaaattgtt gttcaaactg ctgatcagaa cgctgcttgg atttttcttt
    30001 gcgatacatc atacctcttt tatcaattgg aacaattatt tatttctaac aaaatcactt
    30061 catttttgtc agataaataa aa
//
LOCUS       DAJSAW010000031        28197 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJSAW010000031 DAJSAW010000000
VERSION     DAJSAW010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.031"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            160..1302
                     /locus_tag="ONT64_001578"
     CDS             160..1302
                     /locus_tag="ONT64_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR2303001.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            1318..2529
                     /gene="thiI"
                     /locus_tag="ONT64_001579"
     CDS             1318..2529
                     /gene="thiI"
                     /locus_tag="ONT64_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR2303002.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            3102..3749
                     /locus_tag="ONT64_001580"
     CDS             3102..3749
                     /locus_tag="ONT64_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCR2303003.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    3878..4094
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4142..6787
                     /locus_tag="ONT64_001581"
     CDS             4142..6787
                     /locus_tag="ONT64_001581"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR2303004.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            7097..8410
                     /locus_tag="ONT64_001582"
     CDS             7097..8410
                     /locus_tag="ONT64_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR2303005.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            8400..9053
                     /locus_tag="ONT64_001583"
     CDS             8400..9053
                     /locus_tag="ONT64_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR2303006.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            9108..9659
                     /gene="radC"
                     /locus_tag="ONT64_001584"
                     /pseudo
     CDS             9108..9659
                     /gene="radC"
                     /locus_tag="ONT64_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            9790..10875
                     /locus_tag="ONT64_001585"
     CDS             9790..10875
                     /locus_tag="ONT64_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2303007.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            10872..12764
                     /locus_tag="ONT64_001586"
     CDS             10872..12764
                     /locus_tag="ONT64_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2303008.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            12771..13148
                     /locus_tag="ONT64_001587"
     CDS             12771..13148
                     /locus_tag="ONT64_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2303009.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            13302..14084
                     /locus_tag="ONT64_001588"
     CDS             13302..14084
                     /locus_tag="ONT64_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HCR2303010.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(14210..14941)
                     /gene="erm(A)"
                     /locus_tag="ONT64_001589"
     CDS             complement(14210..14941)
                     /gene="erm(A)"
                     /locus_tag="ONT64_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HCR2303011.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(14999..15058)
                     /locus_tag="ONT64_001590"
     CDS             complement(14999..15058)
                     /locus_tag="ONT64_001590"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HCR2303012.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            15450..16112
                     /locus_tag="ONT64_001591"
     CDS             15450..16112
                     /locus_tag="ONT64_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2303013.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            16347..16490
                     /locus_tag="ONT64_001592"
                     /pseudo
     CDS             16347..16490
                     /locus_tag="ONT64_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            16640..16840
                     /locus_tag="ONT64_001593"
     CDS             16640..16840
                     /locus_tag="ONT64_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2303014.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(17094..18773)
                     /locus_tag="ONT64_001594"
     CDS             complement(17094..18773)
                     /locus_tag="ONT64_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCR2303015.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(18775..18987)
                     /locus_tag="ONT64_001595"
     CDS             complement(18775..18987)
                     /locus_tag="ONT64_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCR2303016.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            19380..21110
                     /gene="efrA"
                     /locus_tag="ONT64_001596"
     CDS             19380..21110
                     /gene="efrA"
                     /locus_tag="ONT64_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HCR2303017.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            21107..22867
                     /gene="efrB"
                     /locus_tag="ONT64_001597"
     CDS             21107..22867
                     /gene="efrB"
                     /locus_tag="ONT64_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HCR2303018.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            22962..23159
                     /locus_tag="ONT64_001598"
     CDS             22962..23159
                     /locus_tag="ONT64_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303019.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            23312..23797
                     /locus_tag="ONT64_001599"
     CDS             23312..23797
                     /locus_tag="ONT64_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR2303020.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(23901..24494)
                     /gene="clpP"
                     /locus_tag="ONT64_001600"
     CDS             complement(23901..24494)
                     /gene="clpP"
                     /locus_tag="ONT64_001600"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR2303021.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            24674..25201
                     /locus_tag="ONT64_001601"
     CDS             24674..25201
                     /locus_tag="ONT64_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303022.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            25292..26029
                     /locus_tag="ONT64_001602"
     CDS             25292..26029
                     /locus_tag="ONT64_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR2303023.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            26033..26950
                     /gene="miaA"
                     /locus_tag="ONT64_001603"
     CDS             26033..26950
                     /gene="miaA"
                     /locus_tag="ONT64_001603"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR2303024.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            26952..28181
                     /gene="hflX"
                     /locus_tag="ONT64_001604"
     CDS             26952..28181
                     /gene="hflX"
                     /locus_tag="ONT64_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR2303025.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         9420 a         4609 c         5934 g         8234 t
ORIGIN      
        1 aaaagttagt aaaccagcaa gagcaagacg gattattgtc ttgttcttgt ctttttttgt
       61 atatttgaaa attgaaacaa gtgaaaattt cacctgctct tgaaagcacc ttggacaacc
      121 gttataatag aaaagaattt caaaaaagag gggtttctta tgatatattt tgataacagt
      181 gcaacaaccc caatctttcc gcaaagctta gatgcatatg taaaaaccag tcagcggatt
      241 attgggaatc catcaagctt gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa
      301 gcaagaaagc agatcgctga tttgatccat gtagaaccag aagaaatcta ttttacttca
      361 ggcggaacag aaggagataa ctgggtatta aaaggaacga tgatcgaaaa aagagaatat
      421 gggaatcata tgatcatttc aggagtcgaa catccggccg tcagcgagac agcagaacaa
      481 ttaaaagaat taggaatcga agtaagtatc gccccagtgg ataaacgcgg atttgtacgt
      541 gtcgacgaat tgaaagaatt aataagaaaa gagacagtat tagtctctgt tatggctgtg
      601 aataatgaag taggatcgat tcagcccatc caggaaatca gtgatctttt ggatgatttt
      661 cctaaaattc atttccatgt agatgctgtt caggcgattg gaaaagtacc attctcgcaa
      721 tggttgacag aaagagttga ttttgcaaca ttttcagctc ataaattcca tggcccacgg
      781 ggaactggtt tcatttattg gaaaaagggt cgtcgtttag caccattact gacaggtgga
      841 gggcaagaaa gaaatcagcg aagtgggaca gaaaatgttc caggtattgt ggctatggtt
      901 aaagctttac gtttatcatt agagataaag gaacaaaaac ctgagcagac acaacgctta
      961 aaagtagctc taatcaatgc gttgaagacg tatgagaaag tgacgatttt ttcagaagga
     1021 actgactatg caccgcatat tttatgtttt gctctaaaag gaattcgcgg cgaagttctc
     1081 gtccatgcct tagaagaaaa acaaatattt gtttctacta ctagtgcctg ctcctcacgt
     1141 aaacatatgg caagcagtac attgcacgca atgcatgtac caaatgatct ggctacatct
     1201 gctgttcgaa tcagcttaga tgaaaacaat tcgcttgcag aagtcgagca atttatgatt
     1261 attttcaagc agctttataa gaaattttct gcaatcaatt aaaagaggag tgttcttgtg
     1321 aaatacagtg aaattatggt ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa
     1381 agctttatca tgcaactagc acaaaatgtc agacaagctt tgaaagactt tccagaaatc
     1441 aaaatccatg ctgatcggga ccggatgcat cttttattga atggcgcaga cgggcagcta
     1501 gttattccaa aactagcaaa aatatttggg atccaaaatt tctctccaag tattcgtgta
     1561 gaaaaagatg tcgaagtttt gaaacaagct gttcaagaca tcatgaaaga aatttatact
     1621 gggaaagaaa catttaaaat tatcgctaaa agaagcgatc accaatttga attgacaagt
     1681 aatgaattga atcaaacatt aggtaatgct gtttttgata ttttcccgca tatccaagtt
     1741 caaatgcgtc aaccagatat tccattacgt gtagagattc ggcgtgatgg cgcttatcta
     1801 tcgtatgaaa cgatcaaggg agctggagga ttgcccgtag gaacaagcgg aaaaggaatg
     1861 cttatgcttt ctggggggat tgattctcct gtagcagggt actttgccat gaagcgggga
     1921 gtggaaattg aagctgttca ttttgctagt ccgccgtata caagtgaaca agcactgcaa
     1981 aaagcaaaag atctaacagc caaactagca ccttatgtgg gaacgatcca ttttatcgaa
     2041 gtgccattta ccgagattca agaagaaatc aaaaagtcag tgccacaagg atattggatg
     2101 acaattactc gtcgaatgat gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt
     2161 ttagtcattt tgaatggtga atcattaggt caagtggctt cacaaacttt gcaaagtatg
     2221 gtggctatca atgaagtaac gaatacgcca atcattcggc ctgtagcgac aatggataaa
     2281 cttgaaatca ttgaagtagc agaacagatc gatacattta atctttctat ccagcctttt
     2341 gaagattgct gtacgatttt tgctcctcca cagccaaaaa cacgccctca actggaaaag
     2401 gtccatcaat atgaagagcg tttagcaata gatgaaatga tccaacgagc actagctggc
     2461 ttgaagatgg aagagataca agcagaatcg tctaatgaaa aaactgaaga attttcagaa
     2521 ttgctataag aggttatgaa aatagtgttt tgaccagagt attagagaaa caaaacgaaa
     2581 aaatagctcc atgtatttta ccgtttttga actaatgtcg aagattagct gattgaatac
     2641 catttatcaa gaagttatgg aaaagccggt tcttttttac taactgaaaa ataaaatttt
     2701 atttagagat caagctacag ttgataaatg atataaaaaa gagaatttcc catgaaatag
     2761 cgaggttgtg acagaagtgg gcagcttcaa gaaataagaa ggaatttacg aaaattgctt
     2821 ttcaaatttt tgtgaaattt cggcttattt ttcgaagaag ttacttctgg aacaccgtgt
     2881 atcagaggtt gcgaaaaagc tgccctgcat caagtaataa gaacagccaa acaaaaatag
     2941 ttcctcatat tttacgtttg gttgagctta ttaccgcaga tgcaagcttt tgaacaccgt
     3001 tttatttgtc tcacaccctt ttccttccta cttgtaacct tgctcccgtg ttagaaacat
     3061 gttacactaa atatgtgaaa ttattaacaa ggagcgtcgt tatgcgagaa aaaacagata
     3121 cactgccaaa agcaactgcg aaaagattac ctttatattt acgctacttg aagatgctgg
     3181 atgactcagg tataagtaga atcaaatcaa atgagttcag cgaaatcacc caaattcctt
     3241 ctgcaacgat ccgcagagac ttctcgcaat taggcgagct aggcagaagc ggatatggct
     3301 atgatgtccc ttttcttatc gatgtattca ataatatcct taatactaaa gaagaaaaac
     3361 gaatcgcatt agtaggatat ggaaatcttg gcaaagcatt gaaacacaac aattttagaa
     3421 gaaatgaaaa tctaaatatc gtgtgcgtct ttgataatga tccagcactg atcaatcgag
     3481 taattgatgg agaaatgatc tatccgattg atcgtttcgc ggagatagcc aaagcaaaaa
     3541 atgtgacagt agcaatctct acggtaccaa gtaaatattc acaatctgct attgatgaaa
     3601 ttgtcaaagg aaatgtaacc gctatactta attttgcgcc tgacagagta actgtcccag
     3661 cttatgtcaa tgttcaatac attgatttga caaccgagtt gcagacgctt atttactttg
     3721 atgagaatta ctcagaagtt ttttcttagg aaaatttgag taggcttatt gatcttgcga
     3781 gaactcgtcg taattctttc aaaaattttt tagtactaaa tgaagtggtt ttttcacgac
     3841 cacttgttga ctaaaaaaaa aaaccattgt agaatgaagc ataacgctga acaagaggag
     3901 taactggcac attcattctt agagagagaa ctatctgctg aaaggttctc aatgacaacc
     3961 agtgaaggta gcttgggagt ttctgtcttg aatggagtag agatagacga gtcgtgatcg
     4021 ttaactcact tgagaggcaa taatgattga ttattgcaat ttaggtggta ccgcgtaaat
     4081 catacgtcct agaatacgaa agtattttag gacgtttttt agtttaaagg aggaaaacag
     4141 catgacggaa gaaaataacc tatcaacaaa atataatccg caagaagttg aagctggacg
     4201 ttatcagcat tggttagaac aagatttgtt taaaccgagc ggagataaaa aagcaaaacc
     4261 ttattcaatc gtaatcccgc ccccaaatgt tactggaaaa cttcatttag ggcatgcttg
     4321 ggatacgacc ttgcaagata tgctgattcg tcaaaagaga atgcaaggat atgatacttt
     4381 atggctgcct ggtatggacc atgcaggaat cgcgacacaa gcaaaagtag aagcgaagct
     4441 tagagagcaa gggatttctc gttatgatct aggtcgtgaa aagttcatcg atcaagtgtg
     4501 ggaatggaaa gaagagtatg cgagccacat ccgcgaacag tgggctaaaa tgggcttatc
     4561 tttagattac agtagggaac gttttacatt agatgatgga ttatctgaag cagtacgtaa
     4621 agtttttgtt accttatacg aaaaaggttt gatttatcgt ggtgaatata tcatcaactg
     4681 ggatcctcaa gcacgaacag ctctatctga tatcgaagtt attcataaag atatcgaagg
     4741 cgccttttat catatgagct atgaactagc agatggatca ggcgtagtag aaattgcgac
     4801 aacccgtcca gaaacgatgt tgggagatac ggcgattgct gttcatccag aagacgagcg
     4861 ttatcaagcg ctcatcggca aaaaagtgat tctaccttta gtcaataaac aaatcccaat
     4921 cattgcagat gaatatgtag atatggaatt cgggacaggt gtagtaaaaa tcactccagc
     4981 acatgatcca aacgactttg aggtaggtaa tcgtcatgat cttccacgcg taaatgttat
     5041 ggatgatgat ggtacgatga atgaactagc aggaaaatat gctggaatgg aacgttttgc
     5101 tgctcgtaaa gcaatcgtgc aagatctgaa agaaatcggt cgattgatta aaatcgatac
     5161 gatgatccac agtgtgggtc attccgagcg gacaggtgtc gttgttgaac ctcggctgtc
     5221 tactcaatgg tttgtaaaaa tggcgccgtt agcgaaaaaa gccattgata accaagaaac
     5281 agatcaagca gtggaattct tccctccaag attcaatcaa acgtttttac gttggatgga
     5341 aaatgtccat gattgggtaa tctcacgaca attatggtgg ggacatcaaa ttcctgcatg
     5401 gtatcacaaa gaaacaggtg aaatgtatgt agggatggaa gcaccagcgg atagtgaaaa
     5461 ctgggtacaa gatcctgatg ttttagacac ttggtttagt tcagctttgt ggcctttctc
     5521 aacaatgggc tggccagata cggaaagcga agattataag cgatacttcc ctactagcac
     5581 tttagtaacc ggttatgaca tcattttctt ctgggtaagc agaatgattt tccaaagctt
     5641 ggaatttact ggcgaacgtc catttgagaa tgtcttgatt catggattga ttcgtgacga
     5701 acaagggcgg aagatgagta aatcattagg taatggaatc gatccgatgg aagtaatcga
     5761 aaagtatgga gcagatgctc tccgctggtt cttatcgaat ggttctgcac caggacaaga
     5821 tgtccgcttc agctatgaaa aaatggatgc ttcttggaac tttatcaaca aaatctggaa
     5881 tgcttcacgt ttcgttatca tgaatatcga aggaatgacc gtagaagaga tcgatttatc
     5941 tggagaaaaa tcagtggctg accggtggat ccttactcgt ttgaatgaaa cgatcgaacg
     6001 agtaactgag ctatttgatc gttttgaatt tggtgaagca ggtcgacaac tgtataactt
     6061 tatctgggat gatttttgtg actggtacat cgaaatgagt aaagaagtac tttatggaga
     6121 tgatgctgtt tcaaaacaaa ccacccgtag catcttagtc catacacttg atcagatcct
     6181 acgtttattg catccaatca tgccattcgt tacagaagaa atatgggaac atattcctca
     6241 tcagggaaat tctctagtag tggcagaata tcctgttgta cgtccagagt ttgatgatga
     6301 aacagcttct aaagggatgg aagtattgaa agaattgatt cgagccgttc gaaatattcg
     6361 ttcagaagtg aatacaccat tgtctaaacc aattacgttg ctaattaaaa caaacgatgc
     6421 taaaatcgat caatttttag ttgaaaatac aagctatatc gaacgcttct gtaatcccga
     6481 agagttgacg atatcaagtg agatcactgc gccagatcta gcgatgtcag ctgttctgac
     6541 aggtgcagaa atcttcttgc cgttagcagg attgatcaat attgaagaag aaatcaaacg
     6601 attagaaaaa gaactggaaa agtggacaag cgaagtaaaa cgtgtccaag gaaaactttc
     6661 taatgaacgt tttgtttcta acgcgccgga agaagtagta gaggcagagc gtgcaaaaga
     6721 aaaagattat ttagagaagc aaacggctgt caaagaacga atcgaaagtt tgcgtactat
     6781 tcaataagaa tagaaaaaga gagtgtgaaa tcagctagct attactaaaa gctagctgat
     6841 tgaacatcgt tacaggaaag agtttgagcc tcactcttag cttcgattca taaggagatg
     6901 tgacataaat tttgtcgcat ctctttttta gtctactctt attatgtagg agaaggaggg
     6961 aggtggatca tgtatcaaga gtcaataaag tagttgataa aatcgttcct ctctctgttt
     7021 cttttctctt tgtcatactt ttatttttct tatgtgttct atttgttata ataaaagaga
     7081 aatgggggaa ttgttgatga cgattgaaga agcaatagaa tggatccata gtcgattacc
     7141 atttggttca cgtcctggcc ttgaccgagt cgaagcttta ttagaagtgg tagggcatcc
     7201 agagagaaaa gtaaaaacga tccatatcgc tggaacaaac ggaaaaggtt ctactgtagc
     7261 ttatttgaga agcctattag aagaggctgg gctgacagta ggtacattta cttcacctta
     7321 tatcgaaacg ttcaatgaac ggatcgcaat caatggcaaa agcattccag acaatgattt
     7381 gatccagctt gtaaaaaaat atcagccact gatcaaacaa cttgatcaat ttgaagcagt
     7441 agctggtatt actgaattcg agacattaac agcaatggca tttgattatt ttttgatgaa
     7501 gaaagtggac gtagcgatta tcgaagttgg cttaggcggc ttattggatt ctaccaatgt
     7561 agctgtacca atgctgacgg gaataacaac gattggactt gatcataccg aaatacttgg
     7621 agatacaatt gaagaaatcg ctgcacaaaa agctggaatc atcaaacaag gcgttccagt
     7681 agtaacagga aatattgccc cagaagcttt tactgtcatc caacaaacag ctaaagcaaa
     7741 aaatgcaccg ctttatgtat acggggaaaa ttataaaata aactatctgc atcctgatga
     7801 gatgtgggga gaagtatttg aattttttaa tgaaatagga aagatccctc aattaaccac
     7861 tccgctttta gggcaacatc aagctgaaaa tgcaggagta gccatcgaat tgtattttgt
     7921 ttactgtcaa ttggaaaagc tgcctttccg agaaaaagat gttttgaagg gattgaagaa
     7981 atctcgatgg ccaggaagaa tggagcgttt gagtaacgag ccattagttg ttttagatgg
     8041 tgcccataat acgcatgcaa tgaaccggct tgttgagaat ttgaaaaaag aattcaaagg
     8101 atatcaaatc aacattttgt tttccgcctt acgaactaaa aatgttagag aaatgctaga
     8161 acgattgcag caagtaccaa atgtccatat ttatttgacg acctttgaac atccaaaagc
     8221 catcgaatta agtgaagtat cagatttagc aagtgagaga gtatcaatcg tttcattgtg
     8281 gcaattcgga ttagcagata tattggaaaa gatgacaaat gaagacgtat tagttgtgac
     8341 cggttcactt tatttcattt cagaagttcg ccatttatta atacaattag gaggagatca
     8401 tgaagtataa tgcagtgatt tttgacatgg atggtttgct gttcgacaca gagttagtgt
     8461 attacgaagc cacacaagta gtagcagaca agatgggttt tccttatgat aaagacctgt
     8521 atttaaaatt cttaggtgtt tcagatgaag aagtatgggc gaattatcat atgattttct
     8581 cggaatttgg caaaacaagt gtacaaagat tcatagatga ttcataccag gaaacgcttg
     8641 aacgtttttc gcaaggagcg gtacaaatga agcccggtgt gttggaactc ctctcttttt
     8701 tagaagaaca tcagattccc aaagttgtcg cttccagtaa ccaacgagca gtcattgaac
     8761 ttttattgaa gaagaacaat ctgtttgaac gatttgagac gattgtttct gcagaaaatg
     8821 ttcaacgagc aaaaccagac ccagaaattt tcttgttagc taatcagaag ctaggtacag
     8881 cgaagaaaga aacattgatt ttagaagatt cacaaaatgg tattttagca gcttctgcgg
     8941 ctgagatccc ggtagtcatg gtaccagatc tgcttccacc atcccaagaa ttgatcaaaa
     9001 aaacagctgg ggttgtttct tctttacatg aaatccctgc attgttaaaa taaaaatcca
     9061 tctcttttcg tatcttattt tagataagat agaaaggaga tgtttttttg ggaaaacgat
     9121 tgatcaaaga agtacctact agctctttac caagggaacg tatggaaatt tatggcgcgg
     9181 aagctttatc ggatcaggag cttttggcaa tcttactgcg taccggtcaa cacccttata
     9241 gtgtgatgtc gattgctgga aatctgttaa aaacgtttgg aggactggcc tctttacgac
     9301 aggctacttt gcatgagtta gaagagattc gagggatagg acgcgtgaaa gcaatcgaaa
     9361 tcaaagcctt gatcgaatta ggtcgacgaa ttcagacgga tcatgaaaga gcggctccac
     9421 cggttcgtac cagctacgaa ttggcccagc aattgattct ggaaatgaaa gatcacaaac
     9481 aagaacattt ggtttgtatt tatttggaca cgaagaatca agtgatctta aaaaaaacag
     9541 tgttcatcgg ttcgctgaat cagtcagtcg ctcatcctag agaaattttc cattatgctg
     9601 tacgttattg tgcagcaagg atcgttttag cgcataatca tccttctggt aatgttttaa
     9661 ccatagtata ataaataata gtagttaaag tagtttaaga ggagatatta aagggatatt
     9721 ttactttaaa taaaaattaa caaggtttat aatttgtagt agttaaagta tttaaagagg
     9781 tgggaacatg tgaaggttca aaggatagaa gtggagaata agccgtatcc attgtattta
     9841 ttactagata aagaatacca gctaatagaa ccagtaatga aatttattaa atacttagat
     9901 aatactggta agtctcctaa taccattaaa gcatactgct atcatttaaa gttgctgtac
     9961 gagttcatgg aacagagagg tgttattctt aatgatatta actttgagtt gttagcagac
    10021 ttcgtaggtt ggttgagata tccttcagca tcaaatgtaa ttgatcttca gtcaaaaaaa
    10081 gccataagag aagaaacgac agtgaataca attttaaatg tagttatgag ttttcttgat
    10141 tatttaagta gattaggaga atttaaatca attgatgtat ttaaacaagc aaagggaaga
    10201 aatttcaaag gatttttaca tcatgttaat aagggtagat accaaaagaa tgtcttaaag
    10261 ttaagggtta aaaagaaaca gataagaaca ttgagatcag aggaagttaa gcaaattatt
    10321 gatgcttgtc atacgaaaag agataaatta attttaatgc ttatgtatga gggtggttta
    10381 agaatcggtg aagtgttatc gcttaggctt gaagatattg tcacttggga caatcaaatc
    10441 catttaacac ctagagatgt taatgttaat gaagcttata ttaaattaag gaaggaaaga
    10501 acaatacatg tgagtaaaga acttatgtca ctttatacag attacttgat atatgagtat
    10561 agtgaggaat tggagcatga ttatgttttt atttccttaa aagaaggcta ttttgggaaa
    10621 ccactaaagt accaaagtgt tcttgatcta gttagaagaa tagttaaaag gactggaata
    10681 gaatttacat cacatatgct tcgccacact cacgcaacgc agctaattag ggaaggatgg
    10741 gatgttgcgt tcgttcaaaa gagattaggt cacgcacatg ttcagacaac gttaaatacc
    10801 tatgttcatc tttcagatca ggatatgaaa aatgagttta ataaatacct cgagagaaag
    10861 gagcataaga aatgaatgct tctagtaaaa ggaaaattat tagtcagagt gagattagca
    10921 aaaaaatagc tgtaatgaat gaagaaatgc aggggttttg ggctaataat agttgggata
    10981 taagaaaatg tccacatcct tctgccatag aattaagtaa gaatcctgct ttaaggaatc
    11041 gttgggttcg ttttgaacgt gttaaaaatc tgtggttaag aacagaattg aaatattttt
    11101 atttttacca tttaaacaat ggaatatgga atgcaaaaac tgtctggatt agaaaaggaa
    11161 cagtaattaa taaaatgtta gatttcttag atttaaagta tcctagcatt acttcaatta
    11221 ctgaagttcc tattgaaaaa gcaatgacgg agtatagaac ttatttgaca aaacggggtg
    11281 ttagaattac cactactaat tataagatta ctgctaatca agaaaaaaca cctgtaaaag
    11341 ctaattccta ctatgttact aatctaaaac aatttatgga gttttatgag aacttttatt
    11401 ttgatggaga ggagtgggat aaagacgttt gggatagacg taacttacct ttgccagatg
    11461 ataaggttaa cccaacacaa tatgaatata caattaactt taaagggttt cggaatacat
    11521 attttaaaca acttgtaaaa agatattgta agttgagatt gaacatggat agcttttcct
    11581 atgtaagtga tattgcccaa agacttaaag agttctttaa ttttctggac atgaaattta
    11641 aacaagttca gagagtacac caattaacga gagtggaaat tgaagcatat ttaagtgaac
    11701 taaacatgat gggaataaaa cctagtacaa taactgggag gatctctata ttggaaggac
    11761 tatttagtac ccttcttagg ctagaatggg atgatgttcc ttccaaaata ttaatttatt
    11821 ctgaggacta tccgaaaata ccaagagcaa aaccacgctt tatagatgaa ttcgtcctag
    11881 agcaattgaa cagtcatctt gataaattac ccgaatatat agctacgatg actatgattg
    11941 ttcaagaatg tggaatgagg ataagtgaat tgtgcacctt gaaaaaaggc tgtctattag
    12001 aggacaaaga tggagatttc tttttaaagt attatcaatg gaaaatgaaa aaggagcata
    12061 tagttccaat atctaaagag gtagctttac ttattaaagt tcgggaagat aaagtttcag
    12121 aggaatttcc agatagtgaa tacctctttc caagaaaaga tggatcgcca ttaaaacaag
    12181 aaacatttag aggtgagtta aataaattag cttatgagca aaatatagtg gataaatcag
    12241 gtgagattta tagattccat gcccatgcct ttcgccatac agtaggaaca agaatgatta
    12301 acaacgggat gccccagcat attgtgcaga aatttttggg gcatgaaagc ccagaaatga
    12361 caagcagata cgctcatatc tttgatgaaa ctctaaaaaa tgaatttact aaatttcagg
    12421 aaaaactggt taccaataat ggagatgtgc ttgatctaga tgaagataat gaagtcgatg
    12481 atgtagagct tcaatggttc aagaaaaata taaatgcaca agtgcttcca aatggttatt
    12541 gtagattgcc agtagtagca ggtggttgtc cacatgcgaa tgcatgctta gattgcactc
    12601 acttctgtac cagtaagcaa ttcttaccac agcacgaaga acagttagag cgtacagaag
    12661 agttattagc catagctaag gataaacaat ggcaaagaca agtagagact aatagccgtg
    12721 ttaaagagcg tttagaacaa atcattggaa gtttgacggg gtaattatca atggataaac
    12781 aagttagaaa tacaacagaa attgtacgtt tggcgaagca gaaatcaaaa aagacaaggg
    12841 aaaaagtaga caaagcgatt tctaaatttt cgattgaagg taaagttatt aattttaatt
    12901 caatagcaaa ggaagctaat gtttctaaat catggcttta taaggaacac gatattaggc
    12961 aaagaatcga atcccttcgt gagcgtcaaa taacagcaaa tgtagtctca aaacccaaga
    13021 aaagttctcg ttcggaggaa atccttatta aaaccttaaa aagaagagta atggaattag
    13081 aaaaagaaaa taaaaaatta cagaaccaaa ttcaaaaatt atatggagat ctgtataata
    13141 aagaataatt attaatctgt agacaaattg tgaaaggatg tacttaaacg ctaacggtca
    13201 gctttattga acagtcattt aggtatatgt ccaatctagg gtaagtaaat tgagtatcaa
    13261 tataaacttt atatgaacat aatcagttac gaggtgaaat catgagcaat ttgattaacg
    13321 gaaaaatacc aaatcaagcg attcaaacat taaaaatcgt aaaagattta tttggaagtt
    13381 caatagttgg agtatatcta tttggttcag cagtaaatgg tggtttacgc attaacagcg
    13441 atgtagatgt tctagtcgtc gtgaatcata gtttacctca attaactcga aaaaaactaa
    13501 cagaaagact aatgactata tcaggaaaga ttggaaatac ggattctgtt agaccacttg
    13561 aagttacggt tataaatagg agtgaagttg tcccttggca atatcctcca aaaagagaat
    13621 ttatatacgg tgagtggctc aggggtgaat ttgagaatgg acaaattcag gaaccaagct
    13681 atgatcctga tttggctatt gttttagcac aagcaagaaa gaatagtatt tctctatttg
    13741 gtcctgattc ttcaagtata cttgtctccg tacctttgac agatattcga agagcaatta
    13801 aggattcttt gccagaacta attgagggga taaaaggtga tgagcgtaat gtaattttaa
    13861 ccctagctcg aatgtggcaa acagtgacta ctggtgaaat tacctcgaaa gatgtcgctg
    13921 cagaatgggc tatacctctt ttacctaaag agcatgtaac tttactggat atagctagaa
    13981 aaggctatcg gggagagtgt gatgataagt gggaaggact atattcaaag gtgaaagcac
    14041 tcgttaagta tatgaaaaat tctatagaaa cttctctcaa ttaggctaat tttattgcaa
    14101 taacaggtgc ttacttttaa aactactgat ttattgataa atattgaaca atttttggga
    14161 agaataaagc gtcctcttgt gaaattagag aacgctttat tactttaatt tagtgaaaca
    14221 atttgtaact attgaaaata gaaagaaatt gttccttcga tagtttatta atattagtga
    14281 catttgcatg cttcaaagcc tgtcggaatt ggtttttagt gaaaagaaca cgatattcac
    14341 ggtttaccca cttataaaca aaagatcgat actttttgta gtccttcttt gaaatcaatg
    14401 gttgatgtcg ttcaagaaca atcaatacag agtctacact tggcttagga tgaaaatata
    14461 gtggtggtac ttttttgagc atttttatat ccatctccac cattaatagt aaacccaaag
    14521 ctcgttgcag attttgcaat cttttcgcaa atcccttctc aacgataaga tagctatatt
    14581 tagcctgact ttcaaaggta attcttttga caatatccgt actgatgtta taaggaatat
    14641 taccatatat cttatagttt atatgttttg ggaaggaaaa ttttagaata tccgtttgaa
    14701 tcacttttat attctcagag gggtttaccg cttctttagt cacttgacat aagcctccat
    14761 caatttctat agcagtaact gatcgactca ttttgactag ctctttggta aaatgtcctt
    14821 ttcctgatcc gatttctatt acgttgtctt gtttactgat attcgtgtga ttcaatattt
    14881 cttttacatg ctttttagaa gtaataaaat tttgcgtgtc tttagggttt ttctggttca
    14941 ttataacctt ctccttactg gttataatga actgacatta agcgttcatt ataaccaatt
    15001 aattttgatt tggttgataa cgaactttat taataacaaa tatagaaaaa gtacccatac
    15061 ttttatctcc tttccatttt tttcattatg aatgagataa agtaatttct actactgcga
    15121 tactggtgca cataattgaa acctccttat gtaactgaat atgattgtat catcttttca
    15181 ttttagaata aacctttata tgtaattttg cacttgacac ttcagatgga cgttttcttc
    15241 aaaataatat gttttgaaac ttgataagag aagccactgt ctttactaaa aagtaaaggc
    15301 tgcgataaag gacagtgact ttaataaagt tgcactgttg tattgtcttt ggataaaatt
    15361 gtctaaaggg aagttttatc cccaacgaaa aaacatctaa agtggtaagt aatgaaagaa
    15421 tgtcgataaa gtaggaatgg gggatctttt tgaatacaat agaatcacag tttgataagg
    15481 ttgcagaaga ttacgatttc gtgaatgagc ttttgaatga ttattcattc tttgtgtcta
    15541 atatgtctcc aaagaaaggc agagcattag atatcggatg tggctcgggt ttgttagtgg
    15601 agaaattagc aagttattat gatgaagtgg tagggattga tatttctaat caaatgctcg
    15661 atcttgccaa atctaaacgt caactaacaa atacggtcta tctgaatatg aatgcagaac
    15721 aacttaattt taatgagaag tttgatttta ttgtaagccg aacaaccttt catcatttgg
    15781 atgatatagc cagcgtaata cagcaaatga aggaactgtt gaatgaggaa ggaagaatag
    15841 ttattcttga taatgtatct gaagtcgaga caccgcctac ctatgtttat aaattagggg
    15901 caatccaaga gtttttaccg cattgtttca aatttggaat aaaaaatgcg attagaattt
    15961 ataatcacaa cacatctaaa tcatggcttg agcatttagc ttcagataag tacctatctg
    16021 aacaaaatta ttacgattta tatgaaaagt tgttgcctgg atgccaattc cataaaatgg
    16081 gttgggcaat gggggttgtc tggacaaaat aacttaaagc tgtggctttg aaaagttgaa
    16141 tttgtgattg ttttcagagc tatcgggtgc tttagtataa ataacaatga ataaaaaatc
    16201 agcctgtatc ttatgttact attaagtaat ttaagataca ggttttacta tttttgaaat
    16261 aagtaccttc cacttcaaat acatgtattc aaagtgaaag tattgattta aatgattctt
    16321 ttgtaattta ctcttgataa tatgaaattc catcgcagca ggatatgaat tttaccaaaa
    16381 ggatacaaaa gtgtggagaa atgatgggga tcactgtact ggaccacttg atcatcggac
    16441 gcaaacgata ttttagtttg agagaagaag gaatgatgga agagaagtaa gttgctttct
    16501 tgcaaaaaaa atgaagcaag tgtacaatta tattgtaatt ttgtttggga atagaagttg
    16561 aaacatctgt tgcacctcta tcgtacacga gcaatgttac aatttaaatt agtgcttttg
    16621 gcacgaggag gatattatta tggaaaacgg aacagtaaaa tggttcaacg ctgaaaaagg
    16681 atttggattt atctcacgtg aagatggaag cgacgtattc gttcacttct cagctatcca
    16741 aggtgacgga ttcaaaacat tagaagaagg tcaagcagtg actttcgacg ttgaagaatc
    16801 agaccgcggc ccacaagctg caaacgttgt aaaagcttaa ttttatctgt aaatcaaatt
    16861 gatggtagac atcaattttt acgagagaac aaagcgagta acaaaaatac ctgccacaag
    16921 agacaatttc tcttgtggca ggtatttttt tgtatgaaaa agtaaataaa tttaagaggc
    16981 tatgaaagag ccaccttgta tcaaggaata agaacaaaca aaaatggctt ctcatgtttt
    17041 tcatttgttt tctcttatat tcaggacaag gtgctgattt cataacctct tatttattct
    17101 tcatctggtg tcattaccat tggcaaaatc attggatgtc tttctgtacg ttcgaataag
    17161 aaaggctgaa gcgcgccagt catcgcttct ttcaatttat attctgaaca gtcaggtcca
    17221 ttcaaacatt ctcttaaagc gtggaataag attcgctgag cttcattgat catttctcct
    17281 gattctcgca tgtagacaaa tccacgagag agaatatctg gaccagccaa gatttctttt
    17341 ttcttgatat ccactgtcgc gacagctaat acaagacctt cttctgaaag caggtgacgg
    17401 tcacgtaaaa cgacattacc aatatcaccg atcccgctac catcgatata cacatctcca
    17461 gccacaaaat gcccagcagg acgcgcagaa tcgctagtta acgctaaaac atcgccattt
    17521 tccatgatga agcagttttc tgcaggaaca cctacatctt gtgctagaga agcatgtact
    17581 ttcaacatgc ggaattctcc gtggacagga ataaaatatt ttggcttcat caaacgaagc
    17641 attaattttt gttcttcttg tccaccgtga ccggaagtat ggatgttatt gatttttccg
    17701 tgaatcactt ctgcaccagc ttcagacagc aaattgatca aatgattgac acttgttgta
    17761 ttaccaggaa taggtgaact tgaaaagatc actgtatcgt ctggctggat ttggatttga
    17821 cggtgagtac cattcgcgat gcggctcaac gcggccatcg cttctccttg agaacctgta
    17881 cataggatca tcgtttcatt ggcaggcaag cgattgattt cgtgtgcatc tacaaatgtg
    17941 ccgtctggaa ctttgatata tcctaaacgt tgaccgttga cgatggcatt ttccatgctt
    18001 cgtccgaaaa cagcgatttt acgaccagtt gccacagcgg catctgctgc ttgctgcaaa
    18061 cggaagatgt tagaagcaaa gctggcaaaa atgatacgtc cttcgatttt tgaaaagatc
    18121 ttcaaaatag aatgcccaat cgttttttct gatttagtaa atgttggaat ctcagcattt
    18181 gtactatcag ataaaaggca caagactcct tcttccccga tacgagccat tttatgcaag
    18241 tttgctggtt cgccaacagg agtgaaatcg aatttgaagt ctccagtcgc tacgatattt
    18301 ccagaaggtg ttttcaccac gatccctaat gcatctggga tactatgggt cgttctgaaa
    18361 aagctgacag aagtctttct aaaacggatg atcgtatctt cattgatctc atggagctct
    18421 gcatctcgaa gcaagccatg ctcatctaat ttgtttgtga tcaacgctaa tgcgagagga
    18481 ccagcataga taggaatatt tgcttgtttc agcaagtagg gtattccgcc gatgtggtct
    18541 tcgtgcccgt gagtaatcac caaggctttt actttgtgta agttttgtat gatgtagctg
    18601 tagtcgggaa tgacatagtc aattccaagt aaatcgtctt ccgggaattt gatcccggca
    18661 tcaatcaaaa tgatttcatc ttggaattgt accccatacg tatttttccc gatttcacct
    18721 aatccaccaa tagcaaagac gcctgtctcg ttatttttga tggaaagttt catattaaaa
    18781 ctccactaat gagaaatcag cattttcttt ttcgtactct agatgacttc cttctagaag
    18841 ttgaacgaat tcaatattgt atggggtatt ttcttccaca cgttggcgag cgtctacatc
    18901 gctttttgcc tcaatgtaaa gagattttgt ttcttcccgt ttagggttgc gtacttttgt
    18961 ttcttgataa taaactttat agatcattgt tttgtctcct ttatctgcgt aaagcagtga
    19021 tttagctagt tgattttttt agacaacaca aaacgagggg acaagccacc tggtttttca
    19081 tatgctttga ctagaatact ggattcgagt caaagagaca agcctcgttt ttgcttatct
    19141 gttcggatat tttttcaacc gtaccttctg tataaaaaat atacaacaat taattgttat
    19201 tctagcataa aacagcaaat aagtatagaa atcaactaga ttgaaagaaa aaatcaatgt
    19261 ttatcatccc gctataatca taatgacttt tttatgagag aagtgttcta ttaataggct
    19321 cgttgtgcta tcatttttag taatgaaaat gtaacaaaag attaaaagga gatgagaaag
    19381 tgaaactaat gtggcattat accatgcgtt ataagaaatt cttgtttttc aatttcatat
    19441 gcgtatttgg ttttattttg attgaattag gacttcctac gattttagca cgtatgatcg
    19501 atgtaggaat ccgcaatgat gattttgatt atgtgaagca acaagggata tttatgatcg
    19561 ttattactgt aatcggtatt attttgaata tcttgttagg ttattttggt tcgaggatta
    19621 cgactaatat cgtagcagat attcgagatg atttgttcga aaagatccaa agttattcgc
    19681 atcaggaata cgaaaatcta ggcgtatctt cattgatcac tcgtacaaca aacgatgctt
    19741 atcagatcat gttgttcctg caaaatattt tacgtatcgg ttttatggca ccaatgatgt
    19801 tcattgtcag tttgtacatg gtcatgcgta caagtgtgac actcagtttg tatgtagtag
    19861 gtgcattacc tttactatta ttggcagtcg tagcgattgc taaggtgtct gaaccgttat
    19921 ctaaaaagca acaaaaaaac ttagataaaa tcaacagtat cttgcgggaa aatctttcag
    19981 gattgcgtgt gattcgtgca ttcgtcaatg aaaagtttga agaaaaacgt tttgctgtag
    20041 tcaatgagaa ttatgcttct agttcaaaaa gcttattccg tttgatggct gttgcacaac
    20101 cgggttttta tttcttattt aatatcgtaa tggtattgat catctggagc ggggcagtgc
    20161 aaatcgatgc aggcgagctt cgtgtaggag atttgattgc gtttatcgag tatatattcc
    20221 atgccttgtt ctcattcatg ttatttgcta gtgtgttcat gatgtatcca agagcagctg
    20281 tttcagctgg acgtatccaa gaagccctag atgccaaacc ttcgattgta gaagatccaa
    20341 atggtatatc tgatacaaaa actaaagggt atatcgaatt tcaaaatgta acatttgctt
    20401 atccaggcca ttcgcaagag ccagtgatac gaaatgtcag ttttacagcc tctccaggag
    20461 agaccgttgc ttttattgga agtaccggta gcggaaaatc tacgttgatc caattgatcc
    20521 ctcgctttta tgatgtgagt gaaggaaaga tcttgatcga tggagtagac gtgagagatt
    20581 atcggctaag caagttgcga gataagattg ggtttattcc gcaaaaagcg ctgctattta
    20641 ctggaacgat tgcagataat ttgcgatacg ggaaagaaga tgctacgcaa gaagaaatgg
    20701 aacgtgcagc tgagattgct caagcttcag aattcatttc tcgaaaacca gatggattta
    20761 atgaacattt atcagaaggt ggttccaatt tttcaggtgg acaaaaacaa cgattggcaa
    20821 tagccagagc catcatacgc cgtccggaaa tctatatttt tgatgacagc ttttctgcat
    20881 tagattatca aacggatgcg aagttacgtg ctcgtctgaa aaaagaaacg acagaatcta
    20941 ctgtattgat cgtagcacaa cgtgtgggaa cgatcatgca tgcagataag atcatcgtat
    21001 taaatgaagg agaagtcgtc ggtatgggga cgcatcggga gttgttggaa aaatgcccga
    21061 tttattatga tattgccgct tctcaattat caaaggagga attagcatga aaaaaacctt
    21121 ttcttcctta aaacgtttag ctcgatatat cagaccgtat cgtgagactt ttcttttagt
    21181 gatcctattt acagttctga cagtggcttt caacgtggct ttgccatatg ttacaggact
    21241 tcctactaca gaaatcagcc gtaatattgc caatggcgaa tcaatcaatt tttcttatat
    21301 attcaaatgt ctcatttgga tcacagttgt tggaataggg tattgtattt cgcaattact
    21361 ttctggtgtt ttgatgacaa atgtcgttca aagtgccatg catgatttga gaagagacat
    21421 agaagaaaaa atcaatcgtc ttccggtttc atatttcgat aaaaaccagc aaggaaatat
    21481 tttgtctcgt gtgacgaatg atgtagatgc agtcagcggt gctttacaac aagcttttat
    21541 tggtatcgta aatgcgatat tgggaattac gttggctgca gcaatgatgt tttatatcca
    21601 gccgatgatg gcgctgatct ctatgatcat gattccaaca tcgatctgga tctcaaaacg
    21661 agtgatcaat gcttcgcaga aatattttca aagcatgcag aattctcttg gtgagctaaa
    21721 cggctacgtc caagaaaata tgacaggttt tagtgtattg aaagtatatg gacgcgaaaa
    21781 agaaacattg gaaggattca aaaaagttaa tcacagcttg aaatactttg ggtttcgtgc
    21841 agcattcatt tctggcttga tgatgcctct agttcaatta actgcctatg ctacttatat
    21901 tggtatggct gttctaggaa gcttttatgt tattactggg gcgatcgttg tcggtcaact
    21961 acaagcattc atccaatata tttggcaaat cagtcagcca atggggaacg tgacacaatt
    22021 gtcttcagtt ttgcaaagtg catcagcggc aacgaaacgt gtattcgaga ttttagatga
    22081 gccagaagaa aaagaaaaca aagtagatgc ttcactgcca gaaacgattt taggagatgt
    22141 gacattcgat catgtcgatt ttgcatatga tccgaagaaa ccattaatcc aagatttgaa
    22201 ttttgaagtg aaagctggtc aaaccgtagc tgtggtcggt ccgaccggtg ccggcaagac
    22261 gacattgatc aatctattga tgcgttttta tgacgtagac aaaggagcaa taaggattga
    22321 tggaatcgac accaaatcaa tgagtcgcag tgatgtgcgt tcgctatttg gtatggtctt
    22381 gcaagatgca tggctttatc aaggaacgat tgcagataat atccgcttcg gaaagcttga
    22441 tgcgacagat tatgaagtag ttgatgctgc gaaaacagca aacgtagatc attttatccg
    22501 aacgatgcca gatggatatg atatggccat caattctgag ggagacaatg tgtctttagg
    22561 acaaaaacag ttgctgacga ttgcccgagc agtggtctcc gatcctaaaa tcttgatttt
    22621 agatgaagca acaagttcag tagacacccg tttagaagcg ttgattcaaa aagcaatgga
    22681 tcgcgtgatg gagggacgga caagtttcgt catcgctcac cgtttatcga caatccgtga
    22741 tgcagatctg attctagtga tggatcaagg tcagatcatt gaaaaaggaa cgcatgagag
    22801 tttattggca caaggtggat tttatgaaaa actgtataac agccaatttg ctgaggaagc
    22861 agaataagtc atataagaat aaaacagttt aaaacttatt ttttgttctc tattcttgat
    22921 aatggtaaag taaagttatc aagaaaagtg aggacacgga gatggaacag tttttaaatg
    22981 aaaaaattac tgaagccaaa agagaagttg atcgtttaac gcacgtctgt cagacgaagc
    23041 cttttgattc agctgatttt tttgaaaatg agtttcaaat cgaacgcttg gcagcacaaa
    23101 tagaagtgta cgaattaatt ttaaaaaaag taatagaaga aaatatagat gtacaataaa
    23161 aacaggtaaa acgtcagtta ccagatactg taaatagaca tctggcctga gcaggtttta
    23221 cctgtttttt ttcttatgaa ataaatatat gctatgatac ctttattcaa taggattagt
    23281 ttctacaaaa aatgaaagga gaaaaataaa tatggaacgg tttatagcat caaatgccac
    23341 agtgattggt gatgtgacat tgtcagaaga tgtgacgatc tggtatcaag ctgttttacg
    23401 cggggacagt aattggataa aaataggaca aagaacgaat atccaagatg gtacaatcat
    23461 ccatgtcgat catgatgcac ctgtagatat tgcggagaat gtgacggtag gacatcagtg
    23521 catgctacat ggctgtacaa tcgaaaaagg ggcgctgatc gggatgggga ccacgatatt
    23581 gaatcatgcg gttattggtg agaatagttt gattggtgct ggatcattag taacagaagg
    23641 aaaagttatt cctcctaatg tcttggcttt tggtcgccca gctaaagtga tccgtccttt
    23701 gacaaaagaa gaaatacaaa aaaacaaaga aaacatccag cattatgtcg agataggtcg
    23761 gatgcatgcg caaggaaaat tcaaggagca aagataggca gtagcatcca taaaataaaa
    23821 aaggatggtc agtttgagtt gaccatcctt ttttatttgg tttagataag agagcttaaa
    23881 aaagcttcct ttgaaacaga ttaatttaat gaagaactgt tttccatgac ttcatcgatc
    23941 aatccatatg cttttgcttg ttcagctgtc atatagttgt cacgatctgt atctttttca
    24001 atcacttcaa gcggttgtcc agtgcgttca gctaaaattt tgtttaagcg ttcgcgtgtt
    24061 tgcaaaatat ggcgtgccgc aatttcgatt tcagttgctt gcccttgagc gccgccaaga
    24121 ggttgatgga tcatgatctc agcatttggt aaagcaaatc gtttaccttt tgtaccagct
    24181 gtcagcaaga agcttcccat tgaagcagcc atacccataa caattgtttg gacatctgct
    24241 ttgacaaagt tcattgtgtc gtaaatagcc atacctgctg taactgaacc accaggagaa
    24301 ttgatataaa gatagatatc tttttcagaa tcttgagcgt ctaagaaaag caattgggcg
    24361 atgatcgaat tagcaagatc atctgttact tggccgctta acatgatgat acggtctttc
    24421 aataaacgtg agtagatatc gtaagctctt tcacctcgtg aagattgttc aatgactgta
    24481 ggaattaaat tcataataga gttcctcctt gatttcgttt agaaatagtg taccataatt
    24541 tagtttctaa tacctaaaat gtagtataca tttttggtca aaaaaggtca aataaaaaac
    24601 ttaggcaaat tagtcgcata aacccgcttt atggtatgat aagcagagaa acaaaacaag
    24661 gaggaacaac agattgatag ttggtattac tggccttatc ggagtgctga caggtgctgt
    24721 agttgtctat atcggcatga tactgcaaat gcgtcacttg gaaaataaag aacagcgtag
    24781 acgtgaattg gaattgaaag tcaaagaaat cgagacgctg aatcaattaa ataaaaagat
    24841 caacgagatc ttgcaaaaac ggacaacgct gatggaagaa tatgtcagct ttgatgcatt
    24901 tgatgattgt tatatcacga ttgacgattt tgcgtatctc caatcatttt cagcgcaaaa
    24961 tagtttttat ctgccaaatt atattttaga cgagctattt aagaatatct cccatcgaaa
    25021 agtgatttta tctccagatg aaacaatgaa gattggtgga tatgcttata aaggcggacg
    25081 cgtgatttta gagaatttta cggatagtct gactgaaatg atcaatgaaa aaaaagctca
    25141 gttgaagact atgacgcgcc agccgattgg tttcttctcg aaagatgatg tttggagctg
    25201 atattttgaa gcattgccgt tgcacatgag agcgtggtaa tgctttcttt ttttcttaat
    25261 gatacaatag aactgaacgg agtgaagaaa gatgttacaa atctatgccc atcgcggaag
    25321 cagcggcacc cacccagaaa atacgcttcc tgcatttgca gaagctgtac gagtaggtgc
    25381 agatggtatc gaactagatg tccatctaag caaagatggt tatttgatcg ttatgcacga
    25441 cgaagaagtg gaccggacga ctaacggtaa agggctgatt cgagaaaaaa cattggaaga
    25501 actcaaaaaa ttgaatgcag ggggcaaatt cagcaaagaa tttccagcag ctaaagttcc
    25561 tgaattgaga gaagtgttag gactgctcct ccaaaaaaat taccgaggga tgctaatgat
    25621 tgaaattaaa actgatcagt atgaatatcc cggtatcgaa gaaaaattag ccgaaacatt
    25681 aaaaagtaga gattggcctt ttcaacatgc atattgcagc tttcatttgg attctttgga
    25741 aaaaatcgca ttactggaac cggagataca gctggactta ttgatgagta cttctgataa
    25801 aaagatccaa ctagcagcag aaacccctta tatagaagga gtacatccaa aaaatacttg
    25861 gttgttcgaa catccagtcc aggcagctga ttttccaaaa gcaattcgtg catggacgat
    25921 taacgatgaa gaagaaatgg ccaaatgtca agaactcaaa atagatggaa taataactga
    25981 ttttccggag aaagctcttc ggtggaaaca atcaacgaaa agtaggtaaa atatggaaaa
    26041 agtattagtg atcgtcggtc cgacagcagt cggtaaaaca gcgctaagtg tggaactcgc
    26101 acaactattt ggaggagaga tcatcagtgg tgattcattg caaatctata aaaatctgga
    26161 tatcggtaca gcaaaaatca aagaagaaga gatgaataat gttccgcatc atttgattga
    26221 tgtgatcgaa cctactcaac gttactcagc cgctgatttt cagcattcag gtcgacaatt
    26281 gattaccgag attgcgaacc gagggcacct acctatcata gctggtggta cgggtctcta
    26341 tatccaatcc cttctatatg attatcagtt aggagcgaca gaagaagcga atcctgagat
    26401 tagaaaaaag tatgaaacgt tagctgaaac aattggttcc caagcattat ggaatcagtt
    26461 gaaagaaaaa gacccggcag ctgctgagaa aatccactgg aacaatcaac gcaaagtgat
    26521 aagagctctg gaagttttag agacgacagg acatagtatc acggcaccta aagaagagcc
    26581 aaagcaattg tatgattacc tcatgattgg attagatacg aagcgtagta ttctttatga
    26641 acgaatcaat caaagagtcg atttgatgct agcagaaggt ttagtagcag aagcgcgtaa
    26701 ggtgtatgag cttggagaag tccaggccag tcaaggtatt ggatataaag aatttttccc
    26761 ttattttgaa gggaaagaaa cattggaaga agcaacagaa caagtcaaac tacattcgag
    26821 aagatacgcg aaacgtcagt tgacgtggtt tcgtaaccgt ttggatgcac agtggtttga
    26881 tcttataagt gagccaaatc aaaaaagtca gattgagcaa acaatcaaaa aatggctgga
    26941 ggcggaataa aatggaagaa aaagtgatca tcgtaggggt agaaaccgaa gcaaaccagc
    27001 gttacttttc agagtcaatg gaagagctag ttcagctgac gaataccgca tcaggagaag
    27061 tcgtattcac aattacacaa aagagaccac aagtggatcg gcaaacgatt atcggtaaag
    27121 ggaaattaca agaattagtt caacaagcgg atgcgcatga agcggatttg atcatcttta
    27181 atcatgagct aactccgaga cagagccaac tgattaccga tgctgttggt cttccggtaa
    27241 ttgatcgcgt gcagcttatc ttagatattt ttgcgatgcg tgctcgttcg aaagaaggga
    27301 agctgcaggt tgaacttgct cagttagaat acctgctgcc tcgtttagta gggcaaggga
    27361 aaacattatc tcgtcttggc ggtgggatcg gaacaagagg gccaggtgaa acaaagttag
    27421 aaacagaccg ccgacatata cggaataaaa tttttgctgt taagaaagaa ttgaaagaag
    27481 tagaagctca tcgagagcgc aatcgccaaa aaagaaaaaa tagcgagatt tttcagattg
    27541 gacttattgg ctacacgaat gccgggaagt caactatctt gaatctattg acacaagcgg
    27601 atacttattc gaaagatcaa ttgtttgcaa cgttagatcc attgactaag agatggcgtt
    27661 ttgctgaagg atttgagatt actgtaacgg ataccgtcgg gttcatccaa gacctgccta
    27721 cgcaactgat cgatgctttc cattctacat tagaagaaag tcaaaatatg gatcttttgc
    27781 tacatgtagt cgatgctagt tctccagatc gtattttaca ggagcagact gtcttaaaat
    27841 tgatggatga actgaatatg aaagaaatgc cgatactgac tgtttacaat aaggcagacc
    27901 agatcgaccc agcgatgttc acgcctacgc tattcccaaa tgtattgatt tctactcaga
    27961 cgaaagaagg taagatggcg ttgatcgaag caatcaaacg tcagctgatg gaactaatga
    28021 ccccttatac actttctgtg ccaagcaacg aaggacagaa attaagcgag ttgagaagac
    28081 aaacaatggt tttggacgaa gcgtacatgg aagaaacaaa cgaatatgaa gttaaagggt
    28141 ttgcaaaaac caattcaaaa tggttaagaa aaaaccaata aaattttatt tttttaa
//
LOCUS       DAJSAW010000032        27260 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJSAW010000032 DAJSAW010000000
VERSION     DAJSAW010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.032"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            133..3660
                     /locus_tag="ONT64_001605"
     CDS             133..3660
                     /locus_tag="ONT64_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HCR2303026.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ONT64_001606"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ONT64_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCR2303027.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ONT64_001607"
     CDS             7448..7789
                     /locus_tag="ONT64_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2303028.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ONT64_001608"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ONT64_001608"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR2303029.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="ONT64_001609"
     CDS             9146..11566
                     /locus_tag="ONT64_001609"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR2303030.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ONT64_001610"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ONT64_001610"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR2303031.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ONT64_001611"
     CDS             13000..13737
                     /locus_tag="ONT64_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR2303032.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ONT64_001612"
     CDS             13751..14566
                     /locus_tag="ONT64_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR2303033.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ONT64_001613"
     CDS             14579..15226
                     /locus_tag="ONT64_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR2303034.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ONT64_001614"
     CDS             15242..15898
                     /locus_tag="ONT64_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR2303035.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ONT64_001615"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ONT64_001615"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR2303036.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ONT64_001616"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ONT64_001616"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR2303037.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ONT64_001617"
     CDS             18240..18758
                     /locus_tag="ONT64_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR2303038.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="ONT64_001618"
     CDS             18865..19365
                     /locus_tag="ONT64_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR2303039.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="ONT64_001619"
     CDS             complement(19645..20565)
                     /locus_tag="ONT64_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCR2303040.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="ONT64_001620"
     CDS             20730..21743
                     /locus_tag="ONT64_001620"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCR2303041.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ONT64_001621"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ONT64_001621"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR2303042.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="ONT64_001622"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="ONT64_001622"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR2303043.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="ONT64_001623"
     CDS             complement(23383..24144)
                     /locus_tag="ONT64_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR2303044.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="ONT64_001624"
     CDS             24333..25427
                     /locus_tag="ONT64_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR2303045.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25513..26472)
                     /locus_tag="ONT64_001625"
     CDS             complement(25513..26472)
                     /locus_tag="ONT64_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HCR2303046.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            27034..>27260
                     /locus_tag="ONT64_001626"
     rRNA            27034..>27260
                     /locus_tag="ONT64_001626"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         9004 a         4736 c         5716 g         7804 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 ggttatgatt tgtcaaatta agctagaata aaatgcttct tgtacatagc tcaaaaatat
    25561 ttcgattggt gttctgtaat tcaatgattt tcttggaata tgattacgtt gattgctgac
    25621 actggaaata aatgtctgat tcacttttct aaaatccatt gatttcggca gtccattacg
    25681 acgcagaatc ccgttagaat tctcgtttaa tgggcgttga gaaggtgttc cagggtccgc
    25741 aaaatatata tcaatatcat gttggttact aatggctttc cagttagaaa attcttttcc
    25801 acagtcaaac gtaatagatt taaagaagtt tttagggaag cgagaaaacc attgattaag
    25861 ggcagtttca atatctaatg ccttacggcc gttgggttta atcgtgataa tgactttaga
    25921 taatctttca actaaagtaa tgacggcact tttatgatga atgccaacga tcgtatcacc
    25981 ttcaaggtga ccaaactcag aattgaaatc aggataatta tcaggtcgat catggattga
    26041 gcgctgatac tgttgttttc cccgtttttc ctgatggcca ttgggttttc ttttaccttt
    26101 catcggtagt gtgtcaatat caaatattcc tttagaaaat aaacgataaa gtgttctcat
    26161 accgcatgaa acaggccttt cttttcgccc gataatgacg tcaggcgtcc aaccaagagt
    26221 gactttctct ttaatgtaat ctacttcatg agcaggtaat tgaatgactt ttctaccaca
    26281 ttttttctta ttttctttat actggtgcca ataatcaaga gcagtcttgc cttgcttgaa
    26341 cttattgact acattataaa tagtttgtat agcacgtccc attcggttag cgatttcaac
    26401 cggcttatta tgttgaagat aatatgattc tatcattgtc agttcgtcta tggtaagatg
    26461 tttgtaggtc atttatggtt acactccttt gttttctttc gtcggaaata caatttgagt
    26521 gtaccataaa tgccttttta tttttctaac ttaattttac aattcgcgat atattaaaca
    26581 acgttgtcgc gcttcattga aacattttat gaaaattcca aaaaagagtt gacacatgtt
    26641 tttagacgtg ataagataac taagtcgtta aaaagaacca cttgcgaagc ttgattctat
    26701 cgaaaaaaag cttcaaaaaa gttcttgaca aataacaaac gataagttat gataaatgag
    26761 ttgctgaaag atttcgaaaa acgaaatcga aaaaaacttt taaaaaagag ttgacaatcg
    26821 cttgtctctc tgatatgata taaaagttgc tacggcagcc aaaaacgaat cgggaaacac
    26881 tcccgatgaa gtagaccttt gaaaactgaa caaagtaaga caaaccaaat gtgtagggcg
    26941 tcttgattca attcaagaca ataaacattt ttaacaagca agcaatatgc tagcaaacaa
    27001 attgagctta acaatcgcaa gattgttcga actttttatg agagtttgat cctggctcag
    27061 gacgaacgct ggcggcgtgc ctaatacatg caagtcgaac gcttcttttt ccaccggagc
    27121 ttgctccacc ggaaaaagag gagtggcgaa cgggtgagta acacgtgggt aacctgccca
    27181 tcagaagggg ataacacttg gaaacaggtg ctaataccgt ataacaatcg aaaccgcatg
    27241 gttttgattt gaaaggcgct
//
LOCUS       DAJSAW010000033        26592 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJSAW010000033 DAJSAW010000000
VERSION     DAJSAW010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.033"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            58..456
                     /locus_tag="ONT64_001627"
     CDS             58..456
                     /locus_tag="ONT64_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR2303047.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            440..1135
                     /locus_tag="ONT64_001628"
     CDS             440..1135
                     /locus_tag="ONT64_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR2303048.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1719..2606)
                     /locus_tag="ONT64_001629"
     CDS             complement(1719..2606)
                     /locus_tag="ONT64_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR2303049.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2791..3630
                     /locus_tag="ONT64_001630"
     CDS             2791..3630
                     /locus_tag="ONT64_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR2303050.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3826..4800
                     /gene="sgrA"
                     /locus_tag="ONT64_001631"
     CDS             3826..4800
                     /gene="sgrA"
                     /locus_tag="ONT64_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HCR2303051.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5098..5454
                     /locus_tag="ONT64_001632"
     CDS             5098..5454
                     /locus_tag="ONT64_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303052.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5457..6614
                     /locus_tag="ONT64_001633"
     CDS             5457..6614
                     /locus_tag="ONT64_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2303053.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6683..8023)
                     /locus_tag="ONT64_001634"
     CDS             complement(6683..8023)
                     /locus_tag="ONT64_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR2303054.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(8182..8622)
                     /locus_tag="ONT64_001635"
     CDS             complement(8182..8622)
                     /locus_tag="ONT64_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCR2303055.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            8782..9288
                     /locus_tag="ONT64_001636"
     CDS             8782..9288
                     /locus_tag="ONT64_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR2303056.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            9456..11369
                     /locus_tag="ONT64_001637"
     CDS             9456..11369
                     /locus_tag="ONT64_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCR2303057.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            11374..12375
                     /locus_tag="ONT64_001638"
     CDS             11374..12375
                     /locus_tag="ONT64_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2303058.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(12577..12783)
                     /locus_tag="ONT64_001639"
     CDS             complement(12577..12783)
                     /locus_tag="ONT64_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HCR2303059.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(13429..14277)
                     /locus_tag="ONT64_001640"
     CDS             complement(13429..14277)
                     /locus_tag="ONT64_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2303060.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            14276..15343
                     /gene="gnd"
                     /locus_tag="ONT64_001641"
     CDS             14276..15343
                     /gene="gnd"
                     /locus_tag="ONT64_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HCR2303061.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            15345..16346
                     /locus_tag="ONT64_001642"
     CDS             15345..16346
                     /locus_tag="ONT64_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HCR2303062.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            16343..16627
                     /locus_tag="ONT64_001643"
     CDS             16343..16627
                     /locus_tag="ONT64_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR2303063.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            16660..17457
                     /locus_tag="ONT64_001644"
     CDS             16660..17457
                     /locus_tag="ONT64_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR2303064.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            17471..18304
                     /locus_tag="ONT64_001645"
     CDS             17471..18304
                     /locus_tag="ONT64_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2303065.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            18322..19179
                     /locus_tag="ONT64_001646"
     CDS             18322..19179
                     /locus_tag="ONT64_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2303066.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            19201..19710
                     /locus_tag="ONT64_001647"
     CDS             19201..19710
                     /locus_tag="ONT64_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2303067.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            19747..20148
                     /locus_tag="ONT64_001648"
     CDS             19747..20148
                     /locus_tag="ONT64_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303068.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            20260..20613
                     /locus_tag="ONT64_001649"
     CDS             20260..20613
                     /locus_tag="ONT64_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303069.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            20647..21090
                     /locus_tag="ONT64_001650"
     CDS             20647..21090
                     /locus_tag="ONT64_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR2303070.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            21107..22192
                     /gene="uxuA"
                     /locus_tag="ONT64_001651"
     CDS             21107..22192
                     /gene="uxuA"
                     /locus_tag="ONT64_001651"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR2303071.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            22185..22898
                     /locus_tag="ONT64_001652"
     CDS             22185..22898
                     /locus_tag="ONT64_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR2303072.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(23105..23254)
                     /locus_tag="ONT64_001653"
     CDS             complement(23105..23254)
                     /locus_tag="ONT64_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303073.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            23650..23850
                     /locus_tag="ONT64_001654"
     CDS             23650..23850
                     /locus_tag="ONT64_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2303074.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            24418..24981
                     /locus_tag="ONT64_001655"
     CDS             24418..24981
                     /locus_tag="ONT64_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303075.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(25042..25407)
                     /locus_tag="ONT64_001656"
     CDS             complement(25042..25407)
                     /locus_tag="ONT64_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303076.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(25483..25728)
                     /locus_tag="ONT64_001657"
     CDS             complement(25483..25728)
                     /locus_tag="ONT64_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2303077.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(25798..26052)
                     /locus_tag="ONT64_001658"
     CDS             complement(25798..26052)
                     /locus_tag="ONT64_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303078.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(26216..26539)
                     /locus_tag="ONT64_001659"
     CDS             complement(26216..26539)
                     /locus_tag="ONT64_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303079.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT         9132 a         4154 c         5449 g         7857 t
ORIGIN      
        1 agaaacattt tgaataaaaa ctattagaca aatgatattt tacgaggagg aacagagatg
       61 acgatctttg aaacggttgt aacagatgtt ggtatagaag cagaagtatt caggaaagaa
      121 aaaataatga ttttatttgg agatgaagca ccggaaacat taacagatta ttgttataag
      181 attgaattga atcaggtaag aaaagaaata acttgtgata tgaagctctg ttttgataaa
      241 caaatgtatg taatcacagc agtaggagat gttgtccaaa ctaatttgaa tgaattagga
      301 catatcacga taaaatttga tggatctcat gagccggaac ttccaggaac aatgtatgta
      361 gaagataagg aaatgccgca tttaagaaag gggacaatca ttaaaatcga tcaagaggat
      421 cagaatgggg gaaagctata tggaaatcat gcttgataca gcaaatacaa aaagtattta
      481 ttcttgtcaa cagtttttga atttaacagg cgtaacaaca aatcctacaa ttctgaaaaa
      541 agaaggagcg tcaaacttct tctcgcatat gaaagaaatc aaaaaaataa tcggacagat
      601 tcctatccat attcagacaa taggaagaac ggaagaagaa atgatagagg atgctcgagt
      661 tatcatagaa gaactgggaa aagatacgtt tatcaaggtg ccggtgaatc aagcaggcct
      721 tcaagcgatc aaaaagttga aaaaagaagg ttatttgatt accggtacag ctatttacac
      781 agaattacaa gggtatttag ctattaataa tggagcagat tatattgcac cttattataa
      841 caggatgcaa aatcaaggca ttgatgctat gaaagtgatt gattcgttgc ggactgagat
      901 cgaacgtacg caaagaccaa caaaaatatt agcagctagt ttcaaaaatt gtgaacaagt
      961 cactacagcc attcgatctg gagctcatgc gataacgtta ggtaaagagc tactagatca
     1021 agtattagag atgccctcga ttgataaagc agtgaggggc ttccaagaag attgggtgca
     1081 aactttccaa gcagaaacga ttggtgaact aaaaaaggtc gctgattact tctgaatcaa
     1141 tttctccata aaataaacga tagccttctc gaaaactcga ggaggctatc tgacgattca
     1201 atattcatta atttaaagtt tgggtgacta ttacttattt ctcatttaaa cgatgtattc
     1261 cctaacctct aatacatcct tgtaactctg aagttacgcc agttttgaat aaatttcata
     1321 aaaacaaatg aaaagtgtcg cataaaaata tctttcgtgt ttctctagta taacagaata
     1381 agaggaacaa gtccagaagg gatattttta gtctttttta aaataacttt tcagtcgagt
     1441 ttgagtttat gatagttgtt tttgaagcga tacggcaagt gaaaaaatag ggaagcatga
     1501 aagaatctac ttaatggcgc attgattcat gtgatagaaa taaaaaactt ttttaaatta
     1561 tatcctcatg attgtaacaa atccagtgat ttgttttggt acaaataaaa aaaggccccg
     1621 atgaagttgt ggcaaaagtg gtttgcctca aaaataagaa ggaatttcca aaaatcactt
     1681 ctcaaatttt gccacatccc caattgggtc aaatttgttt atttgaatat attttttaca
     1741 taaggatctt taatagttac attgtctttt ttcatcactt tacggattgt tttatccatg
     1801 aattccgtat caagagtttt tgcttcggta gcaatttttt ctagctcaga tttgtatttg
     1861 tctttattag aacctttatc ctgttttttt accattttta ctagataata gctttgctga
     1921 taagtcgttg gatctgttac aggaatgata tcagaaactt ccccgttttt tagtttaaat
     1981 gctgcttttt ttacttcagt aggaatctct ggattggttg aatcaaaatt catttttcca
     2041 tcagtttcct ttaatgtttt attttttcca taagcttgaa caagcttgga gaaattttcc
     2101 ccttcatttg cttctttttt tgctttatct gcttcttctt tagtcgaaaa tagaatgatc
     2161 tgtgtactaa cttctggata gaattctttc caagcagtgt ctagatcttt atctgttaat
     2221 tttacattgg ctttcaaacc gtattggaaa gccagcatct gtttaaagag tttcttatat
     2281 gtctcttctg tttggccaac tgcttttaat tgttccttgt agtttggacc gagttttttg
     2341 atttgttcat tatattcttt tgttacttcc tttgaagaaa tctcttttcc gtatacatca
     2401 tcaaatgctt tgaagacaat catttttttt aatagtgttt gattatttga tttaactttt
     2461 gggttatcat aaaaatcctg aactgtaagt gttccacctt taaacgttgc aatcgtttca
     2521 ttggacttgc tacaacctga gaatagtaaa attgcacatg ctgaaaggca cataattagt
     2581 agttgttttt tcaaaaaatt tcccatcctt tccttattaa aaacagtcta acagaagaat
     2641 tagggagaca gtctttcatt ttcgtaaggt tgctgtagat attcttagtt cgtaaaaatc
     2701 tattctctct atgatggaga acatttacat agttttcacg agacaagaaa gaaaagggtt
     2761 gttaaactta atataaagga gggatgaaag atgatcagga ttattcggtt gattggatct
     2821 gttttaatag tcttagttgg ttttgtttta ggaattcttg tcaataatgc acttgcggat
     2881 atgtttattg gaaatagtga atggattgga gcaattgccg gtacggtcgg acatttagct
     2941 gtttttttac tagctttatt tcttgctagt aaggtcgaag gaaaaagaat agaagattat
     3001 ggtgtttctg ttcgaggaaa agactggggg tatttagcgg gaggtctcgt tgttggaatt
     3061 ggagtgtttc tgcttattac tagtccatta tatttgacgg gggcgtatcg gttagattca
     3121 ggaaacgcaa acattgtgcc gctgataacg agttttattt tatttattgc agtgggggca
     3181 tcagaagagc ttttattccg tggctttttt cagcatcaga tgcttagatt tggtccgtta
     3241 atagcaatga ttggatctgc tgcattattt gctcttttgc acggattgaa tcctaacatg
     3301 acatttctgg cagttttcaa tatcttttta gcaggttgct ttttcagcgc acttatctac
     3361 agtacaggaa gtttatttac ggctattggt gctcatatta cttggaattg gacacaagga
     3421 gctattttgg gtataccagt cagcggaacg caagaacaag gttttttctc cactctgatc
     3481 caatcgcaaa atacaataat tactggggga aactttgggg ctgaagcatc tatatcaact
     3541 acattattct tgttagtctt gattgttggt ttgttactct atgcatataa aacaaagcgg
     3601 atgaagaaat acactgctgt ttatcaataa aagatgaaga aagcaaggct gatatcggtc
     3661 ttgctttttt aagtctgtca ttgattaaac gaaaaagtaa acaaattcaa gcgaatacac
     3721 gacatctttt gttagatttt cacaactgac tgtcgtgttc tcatcatttt cattttatat
     3781 aattccttta tcaaatagaa ggtaattata tggagagtga tagaaatgaa gaaaacagcg
     3841 accgtattat ttgttattct tgggctgagt ataattgcag caatgattgg aatcagtact
     3901 tctgcaaatg aacgggcaaa tgagaatctt tcatttactg tgaaaactga tcggattgtt
     3961 tatgacatga cacaacaagt cattactatt ccagtcaaac caaacaaaag cgtgaatgct
     4021 agtgatgtac atgctgttct aacatatggt tgggatggta atggaagttc agaaaaagtt
     4081 attggggaag tttatttaaa agacgtacaa tggaccgctg gtattgagta tacaataatg
     4141 atttctgcag aactatcgat tgatgagatc aagagtaaag ataaagttga tttgattgtt
     4201 ttttatgatg gacagatgac gatcacggag aatttgaaac caagcagttg gacagttgtt
     4261 ggaccgccaa gcagtacgga tagcacaagt agtgaaagca gtacagaaaa cacgagcggt
     4321 gaaagcagta cagagagtac gagcagtgaa agtagcacag aaaacacgag cagcgaaagc
     4381 agtacggaga gtacgagtag tgaaagtagc acaggaagca cgagcagcga aagcagtacg
     4441 gagagtacga gtagtgaaag tagcacagga agcatgagta gtgagagtag cacaggaagc
     4501 acgagcagcg aaagcagcac aggaaaagta aataatgaac ttgatttggt acctatcata
     4561 ccaactaagg aacaaaagag aaatgcaggg agcaacctaa aaagtgtgct aaaacagcct
     4621 gattttgttt ctcgtggaat tgatcatcaa gaaaaagcga aagaaacaga taatagaaaa
     4681 agcgatttac cagaaactgg tagtagaacc ttgaacaagc taattacatg gatgggtgtg
     4741 ttactcattt tgattgtagg agcaagctat tttcgttctc tcttccatcg agtgaaataa
     4801 agaataaaag caacagatct aaaattgaat aaaacgatag atttttgagt gaatatttca
     4861 ttcgagagtg ctgtattgcc tggcgaagtt gtagctttgc cagatctatt tttgtttact
     4921 aacatatttg agaaacagga aataacagga acacaaatgg aaaataagag caagaataat
     4981 tgacaatgca cactgcgcat ggtagtatct ctgaaaaatc agtaacgaat ctatttgtac
     5041 gttattcatg tagctgtcgg aataagtcta aataatagat aaaaaggagt atgtactttg
     5101 tttgaagaat ttattgatat caatgaaagg caagtatatc aatttttgaa ctattgttat
     5161 gaaagagacg agaaattata tgtggtgaag gatatcgctt tagatctaaa ctacaccctg
     5221 gctaagatga attcggtgat tcaacaagca gaatcgtttt gcgaaaggta tccagaatat
     5281 aaattatctt ttctttcaga aaacaagatg ataaaagtcg agttttcttc tcagttttta
     5341 ctgagtaaag tttattctat acttttagag ggaacgattg gttatatttt actggattca
     5401 ctttacaaag gtacttatca atccttagaa aatcttagtc aaaaaattat ataagtttgc
     5461 gtacgattca aagaaaatta agagaactta atactatttt gaaaaactat catttatctt
     5521 gcagcttgaa aaaaggtaat ccgatagaag gagaagaata ccgtatccga tacttttttc
     5581 acttgatgta ttggcagtca ttcgacgaaa atcattctga tatattatca agtataaaaa
     5641 ataaacgtca actattagaa aaatttgcga aacatgcgcc atatacgcgt agtatagata
     5701 gaaagaaatg gatcagccta ctaagaattt cgttgcaacg aatgaaacaa ggccactcga
     5761 ttacgtatgt cccagaagag atgaacaaat ttgtgcatcc attgataaca aaagaagagt
     5821 ttacgcatta catactagag cctttttttg aagcaaactt ctggtcgatg aatcacatca
     5881 ctccaacaga gcaagcttac ctttatttta tgtttggtgt aatgaacacc tatttggaag
     5941 aagatttgtt ggatactcca gataaagtac aagaaagttg gggctggatt gaatcgtcta
     6001 ttgtagaaaa ttttgaaaaa aacttcaaga tttgttttac aacttcagaa aaaaattatc
     6061 tatatgtcaa tttggcaatg atccatttgt attcaagggt ttttgggaca aaaaagaagg
     6121 tggatgcttt tggaaaatca gcagaagaca gagattttca gcatatattt ccagtgatgt
     6181 atccaatcat gcaacagttt tttagtgggg tagctcgcag aattccagag cttaatactt
     6241 attatgaaaa gaacagacgg ttgatttttc aatactgtat gttagtcagg gatattttta
     6301 taaaacacga acaacctgtc atattctata ttcaatcaaa gtatggtaaa acacaggaat
     6361 tatgggcaaa gaaaaggata ttatctatga ctgaacgccc aataaaatat agtgcatccg
     6421 ctgatcagtt acctgatatt gtcattagcg attatcctat cgacaaaaag gcatatgatc
     6481 aaacgaatac acagatattt tactggaatg gacaacctac aaaaaatgat tgggcgcgtc
     6541 tgaaagctga aatcataaaa atcaggaatg agaaaaatag atccatattg gaaaaattgg
     6601 atttcgttaa ttaagaaaag cggctcttcg tcaatgagtg ttggtgaaga aattcaatag
     6661 aataatcgag atactcacac ggctagacag ctatcttgtc tagccgtgtt gttgttctgg
     6721 ctttagtcag ttgggtagtg ttcttagttt tcttgaaata ttttcctcga tataaaaaga
     6781 ttcgttctcg gtagttttga aagtttctaa agccataggc aattcgtttt agtactttga
     6841 tatggttgtt caaacattct atagggccgt tggaataggg aactcgcaac gctcgtttga
     6901 tttctgttcg atatttcttg aaggtcgtga acactggttg atagtgctct ggcaactcct
     6961 tgtagtcttc ttttaggagc gcgacgaatt caggaacatc ttgtcttctg atagcggaaa
     7021 gaaaaacctg gtagacttcg tagcctcgct ttaaggagtc atcataatat aatagccgat
     7081 cgactatctc cgcttctgtc agatgatctc gaaatgacgg acgccagtag cgatgctgac
     7141 cattcaattc ccacgcattc ttttgaagta gtctccagta tttctttaag tgcttcgacc
     7201 gacgatcccc ttttttaaag gtcttcatta cttgaattcg gtggttggta aaggcccgac
     7261 cgatgtgttg gctaatatga aagcgatcgg cgacgattcg ggcattcgga aaacactctt
     7321 tcaccaatgt gcgatagggt gtatagaaat ctgaaaccac tagacgtact tgttcacgga
     7381 cttttctagg gtagcgcaag aaatagtcgc gtaaaggatt gagttgccga ttttccacaa
     7441 tatcaatcag ttgatgggtc ttgccgtcca tcatgacaaa gctcatggct cccgagacgt
     7501 ttttcaccga tttgaattca tcgaaacaca ccacttctgg taacggctgg ttcaaagaaa
     7561 cggttttagg ccgaaggctg cgaagtattc gaatgacagt ggtcggagaa atgtgcttca
     7621 tacgtgcaat caaagacata gaaataggct cacttaatag ctcaagaatg gcttgtttga
     7681 ctctgcgggc aatggagcag cgacgatcgc taactgaatc ttctgctaaa aaagtctttc
     7741 cacagtgttt acatttgaag cgctgtttgt taatccgcac aatggtcttg tagttactga
     7801 catcattcaa taagatggat acttttttcc agccccattt aatgatcgtt tgcttatttt
     7861 gatagtggca atggaagcat tcctctggtt gataggtcaa tgtgccatca aaaacaaaac
     7921 atgtttcgtc attaatgcgc tctttgcgaa aggcatcttc atgaaaggta agatttaggt
     7981 ctagaatatc tagtaactct ttggtaaact ctgaatagga catcttgaaa cactcctttg
     8041 aatggttttc gtcgacttta ttctaaagga tttcaaggtg tctgtttgcg tttattagaa
     8101 aaaagaaaaa gtattggcaa cagaaggcgt caccaacact agatattata gatccagaaa
     8161 agcagattgt ttatgccaaa attaagacat tgcaaaaaga ttattttctt taaaatcgac
     8221 aagatgacca gaaagcaaga gttcgttgag ctcttgcttt cttttagttt tagaaaggaa
     8281 catcctgatt ttatcttcgc taaatccagt caaaagtttt gcagattggt cgtagataat
     8341 cggttttttc aataaagaag gggatgtacg aatctcaata tatagttcac tcaaactgag
     8401 ttcatctatt ctgtgaacaa gatttttata actaatagaa tgagtagaga gcatttcggt
     8461 cacgccgttg tttgtcaatt ttagtaagtg gataaattca tttttggtta gtggttgacg
     8521 aattaagttc tgttcgataa acggtatatc atggacgata agccaacgtt ttgcgcgtct
     8581 tgaagacgag cagccagaac ctgtatacaa tttaatcatc atacattcct ccaatagcta
     8641 agtatttatg aaataataac atgaagaaat agtcgaaaag tgccatttag aggtgtaaaa
     8701 taagaaaatg ttgtcacgta taatggaact agctagataa gaaatactat taaatcaaga
     8761 aaaaagaaag aaggcacaag tatgtcaaac acagccgacg caatagtagt gatcgattta
     8821 caaaaagggg tatgttttga ttcttcgccg atacacgaat tagaaacagt gacagcatta
     8881 gtgaataaaa gaatcaaaac atacgaaacg attgggaaac cagttatttt cgttcaacac
     8941 gaagatgctc aattagtgcc acaaacaaaa ccatgggagc tattgtcgga attatctatt
     9001 cctgagcaca gttattttgt gagaaaaacc catgcgaatt cattttataa gactaatttg
     9061 agtgttttat taactgaatt ggactgtcat tcgatcgaat tatgtggggc acaagcagag
     9121 tattgtatag atacgactgt gaagtttgct catggattag gataccagct tatgatgtgt
     9181 agaggagcaa cgacaactta tgataatcct tttatgaaag caaaggaaac aatcgctttt
     9241 tacgagaaaa tttgggataa acgatttctg acatttttgg agcaataagt tgtttcttcg
     9301 tttaaaaaaa gcagaatgga tactggttta tccattctgc tttattacgt aaaatgagaa
     9361 aataatcctg ttctattttt atgggataaa ccatttattt tttgtataaa aaatgtttgc
     9421 gtttacatat aattctccta gaaacataaa aaggagtgaa tagcttgaga acaaaacaag
     9481 tattaaaggg agcgttggtc atcttatttg ctgtaagtac aagcgtcccg caatggacag
     9541 tatatggaga gagcatttct tccgaatcgg aatctcggga ggtagaaacc acagaaacta
     9601 ctgatgctgg aaatgaaacg aaatcgagta cttcacaaac agaaacaaat actgaaacaa
     9661 cggttgagac atctgttact agcacagatc aagaaacgac cgctaccaca gataaccaac
     9721 aaacagaaga agcaacagct acaaaaatgt caaacgacga atcctcttct gctaaagtaa
     9781 gtgacaaaga gaatgggaca gaaaatgatg atatcggtaa aggtgaaaat ttcaatggta
     9841 aagaatattt gaattacttc ggtaaacaga tgacagaaat acttaatgga ccaacagaag
     9901 acatcaagtc aaaattaata gagttattag tcactgagct tgctgttaat gattatggtg
     9961 tagataaaga gattatcaaa catctgattg ctacttatct attgtacgat atcgcttttg
    10021 caaaaaacag tgtgttgatg tcgatgcttg gaggtacaga tgatgggaca acgcctacag
    10081 aaaacacagg cttctatcca aatacaaaac aacttcatca aactgtacta aacaaacaag
    10141 atggcaaaat gatggatgta tatgcatatt atgtcgatca aggttcagat aaaacagtca
    10201 tgatccatgg cggatttcga gggaattgga ataacgggat cgttacagaa gaatacaatg
    10261 atttttataa ggccgggtat aatttattat ttgttgattc cagagcgact ggtaatagcg
    10321 gaggcgatta tgtgacctat ggacaatatg agtcagatga cgtcctttac tggataaatc
    10381 aagaagttcg agagcggcca tctcaaaaga ttttacttta tggtggttcg atgggagcag
    10441 ctacgatgat gtctgtatta gcgaaagata ttcctgtcaa tgtcaaagga attattgaaa
    10501 attgcggatt tgcatcaatt gatgaacaac tacgttttac gtacagccag acagtagccc
    10561 ccgcactacc ggatgccata aaaaatcagt tagacatcat tggggatcaa gagcatgaag
    10621 atttatttat gggccttttg aaacaatact attttgatca ggagatgcat ttggatacaa
    10681 aagctgctct acctacgatt ggaatgtcag attcattgcc taaattgatt atccatggaa
    10741 cagcagatga tgtagtacct gtgtctaacg cgcaaaaatt atatgagtta tcaggagggt
    10801 ataaagatct tttattagta gaaggagcag ggcatggaaa ggcacaggaa gttgaccatg
    10861 ctgcctacac gaaacatgta acggactttt tgaaagtagt gttccatgat cagataaacg
    10921 taaagtatgt ggatgaaaac aataaaagtc tactcaaaga ccaggacgaa atccagcttt
    10981 acggagctta tggagaaaat tatatgacag agcaaaagac attcgaagga tacgaattag
    11041 caaatgttga aggaccgact gaagggattt ttaatgagac gataccaact attattttct
    11101 attacaaaaa aatcccagta gctccaccaa aaaagcaaga tccgacagag aatgcagaca
    11161 ataagggaaa gaatgaacct cgagaggatc aatcaagtga tgatacgcaa ccgattcaga
    11221 tgacagtgaa agtacaaaaa aagacagatg caaaaaaaag aatgttgccg aaaactggag
    11281 aaaagaaaca ttcgcttctc atttattcag gtggattttt atctgttagc acgattatca
    11341 catggatttg gcgaagaaag agagggtaaa tgaatgaaat ggttcaagcg aatagtgatt
    11401 gcagtaccag ttatcatcgt agttatttta gtcactgtat ttttgattaa tcaaatcaca
    11461 ccaaagccga ttagcttgct tgttagaaaa caatttgaca caagtgaaaa agaacaagta
    11521 tatgcccgac cagatgattt ccagcaaaaa atcgaacaaa tcgatataaa aaaagaaata
    11581 tcctatcctt caaagtacaa aaataataat atggatatct acacaccgaa agtggaaaag
    11641 aaaaaattac cgattctttt ctggatgcat ggaggcgcat atgtaggagg ggataaaaat
    11701 gattgtcgtg attatttgga atatctatgt tcagacacgc agcaaatcat cgtaaatata
    11761 gattatgaac gatcaccgga agcaaaacat cctacaccag ttatccaatt gaacgaagcc
    11821 attcaagcag ctaaaaaaga gataaaagat caagcagatt ggtcgaatat ttcgattggt
    11881 ggaaattcgg caggtgcaca aattagtgga gaatatttgt tagcacttca aaatgaagaa
    11941 attaaaaaag ctgatcatct tgatccgact ttggagaaca agcagattac gaagtttatt
    12001 tctttgtctg gtctgttgga tccttctgca cttactcaag taagtgataa aatctctagc
    12061 tttctttatg aaaaatgtgg atgggcttac tttgataata aggactttga acaatcagag
    12121 aaggtcaaaa gtttagcctt ggtcagacat gcggatcgtt ggacgcaaaa cacattctta
    12181 acggatggaa atacaaatac gtttacgaaa caaatggaag aagtaacttc tgtatttgag
    12241 aaagcggggg tgaaagtcac aaaagtagat tatgcggaga aaaatgctgt tttgaaccat
    12301 gaatatcagt ttgatttcag caataaacaa gcgcaagaaa catatcaaaa gttagtaatg
    12361 tttttcaagg agtaaagtct tatgatgcaa aagaggttgt aatctgtcac ttagctccaa
    12421 gtcacaagga gcaatttcaa taaacaagct tttcatggtt tatgaatatt gtaacttgtg
    12481 actgaggagt tagagatggc aaggggcacc ttttattcga aatttgggaa tatcacacga
    12541 ttattgtcac gatctctttt acgtaaattt tataagtcag atttgttgtt ttgatcgttt
    12601 aaaaaaagca acaaatccga agcctgcgga ggcaagaagg gacaaaaagc cgagtggtaa
    12661 gcaaaagaat gctggttcca ctaatattcc gttgttattg atataaggct gggaacaaag
    12721 atgaatagca aataaaatca atgcactagc gaaaaaaagc aaagaaatat ggatagcttt
    12781 cataagcagc acctcctttt ttataattgt atgataaata gattaagaat ctataaatgt
    12841 gacaagtttg ttaggatgtg aataaaatga tcatctagcg aacttgaaac aaagatggct
    12901 aatgataagc aattaatgat tcagcaatca gataggtaca aagtttacca tcatacgtct
    12961 agaccaataa tagaaaatat gggaatataa tagaaaagat ttaggatacc cccaaaagaa
    13021 tttccccaaa aattcgatgt atcctaaact tttttatttt acataacaat catatcaact
    13081 gtgaaataaa attacaaatg ataacctaaa atgaggccaa aagccagtgt tatcaaggaa
    13141 atacttaatt caccaaaatt ttttttaatt ttttagaaaa taagaaacaa aaataataaa
    13201 aaaaagaaaa gataaatttt ttactactgt atactaactt tgttatcgca tctaacaagt
    13261 aactcgaata ataccaatac aaccttctcc aaagatgcga tgacatacgt tctaacccac
    13321 cgactattga tgttctgtac cacccttgca gaaccaaaaa agccactacc aaatatttgg
    13381 tagtggcttt ttttgtttta tggatttttc gctttttctg ttatggtgct attctttgat
    13441 ttcagaaata gtatgtcgaa ctttagcgat agaacgacgt gattcttttt cgttgtaaaa
    13501 catcaaaatc acatatagag agtccaaaat gcttagttga gaaatacggg aagctaaagc
    13561 ttcagatcgg aattctgttt cttcagaaat agaaataaaa acgacatcgc caagtttagc
    13621 aagggaagaa ttagcttggc tagtaataac gattgttttt gctccagttt ttcttacggt
    13681 ttcagcaata tggattgttt cttttgattg ccctgtgtga gaaatcaaaa cagcacagtc
    13741 tttttccgtc aataaagaag cttccatcaa ttgaatatgg taatccgtgc tgtgagaagt
    13801 tgcgataggg gaacgcaaaa atttatgata agcgtcagtt gctacaatct ctgaaccgcc
    13861 aacaccgaaa aaatagacac gtttagattc attgataata gtagccgctt tttttagatc
    13921 cttttgttgt aacaactttt ttgtgtcatt taaagttgaa atactagact cgaacacctt
    13981 ttgcgccata gtgagctcag tatctgtttt ctgaatattt tcgtggatag aaatagcaga
    14041 tagatcattt tcttggatca acatggccat tttaaaatct ttaaatccat tataaccaag
    14101 tttttttgta aattgaaaaa aagtagagtc agcaatgccc agttcacttg ctaaattgct
    14161 aataggacta tgacttactt tgcttggatt ttctaaaata taatcggcga tagcctgttc
    14221 ttttgggctg aagtcgttgt acattgtttt gatttgtagt cttatggaga cgctcatgag
    14281 aattcctcat tttcataata agattatcat tttttccgtt ctcattaatg ttaaagatac
    14341 aaggagagta ggaaaaaatc aaacaaaaaa tatttttgta aaatctattt acaaaaaaat
    14401 ttttttgtat aatgagggta taagaaatgg aggcgctatc atggaaattg gtgtaattgg
    14461 tttgggaaaa atgggattga atttagcttt aaacttaaaa gagcaccatc acaaagtaca
    14521 aggactcgac atttcaagta cagctgttag tcaggcaaga gaaaatgaca ttcctgctta
    14581 tcaagagatc tctgaatttc tagctacttt tgagcagaaa aaaatcatct ggttaatgct
    14641 tcctgctgga gagataaccg aaaacatgtt aagaaaactt tttccaaaac tcggaccagg
    14701 agacattatt attgaaggcg gaaactctta ttataaagat tcaatccgtc gagcagaaga
    14761 gtttcagaaa aatgaaatcg gttacttgga ttgtgggact tctggaggaa tcgaaggagc
    14821 acgacatcaa gcctgcttaa tgattggagg agataaagaa acttttctat ccgtggaaca
    14881 attattcaaa gatgtagcaa ttgaaagagg atatctgtat gctggtgcat ccggaagcgg
    14941 acacttcttg aaaatgatcc ataatggaat cgaatatgga atgatgcaag caatcggtga
    15001 aggattccaa cttgtacaaa aaagtgagta tgcttttgac ttggaacaag tcgcacgtgt
    15061 ttggaatcat ggttcggtga ttcgcggctg gctgatggaa atcatggaag atcagttaaa
    15121 cgaacatccc aatttaagta attatcgggg aatcgtttct gcatcaggtg aagcaaaatg
    15181 gacggttgaa acagcgctag aaatggctgt tccagttcca gtaattgcaa ctagtttgtt
    15241 catgaggaat ctttcacagg aagaagatag tttttcagca aaagtagttt cagcactaag
    15301 aaatgggttt ggcggacatg aaatagttga aaagggtgag taaaatgcca gattatcata
    15361 tagcaatcgt taattccagc agtttcggaa gagtctttcc acaccacttg gaaagattaa
    15421 agtcagttgg agacgttcat ttctttactg ttgatcaaga gattccagga aaagaactag
    15481 cggagttact taaaggattc aatatcatta ttgcaagtgt tacgccattt tttcgacgag
    15541 atttttttga aaacaaagat gaactgctat tgatttcccg acatggtatt ggttacaaca
    15601 atattgattt aaaagcagca aaagaacacc agacaatcgt atctatcata cctgctttag
    15661 ttgaacgaaa tgctgtagca gaaaataata taactaattt gttgtccatg cttcgacgca
    15721 cgacacaagc acaagcaaga gtcaaggacg atcgatggga agatcgggcg gagttcgtcg
    15781 gtaggacgct gtttaacaaa acagttggtg tgattggtgc aggaaatacc ggaagcggag
    15841 taatcgaaat tttgcgtaac ggttttcgct gtgatgttct agcttatgat ccttacaaat
    15901 cagatttata catcgaaagc tttggtgcaa aaaaagtaga acttcaagaa ttactggaac
    15961 aatcagatat catttgttta tgtgcgaatt tgacagaaga gaactatcac atgatttctg
    16021 ataaagaagt cgatcagatg aaagaaaatg tttatctatc taatagtgca agaggcgctc
    16081 tagttgatga agaagcgatg attaatggtc tgaaaagcgg aaagatcgca ggatatgcca
    16141 cagatgtttt agaagaagaa ccgggacgtc aagatcatcc gtatttagcg tttgaacaag
    16201 tagtgattac gccacatata tcggcctaca caagggaatg tttagaagca atgggtgaaa
    16261 aatgtgtcag agatgtcgaa cagatcagtc agcaccttct tcctgaacgt tctgtccaga
    16321 caaagagcaa ctatataaaa ggatgatgaa gaaaatggtg aacggtttaa aagtgaaaac
    16381 aggccctcaa ttttatttat atgaagaagg tggtatttca aaagtttctg atctcttgaa
    16441 atcatacggc gcgaaaagag tactggtcac ccacggaacg gtatcatggg aaaaggcgct
    16501 acctaaatta gtatttttga atgacgaaac gattcaattt ttttatcatc gttattctgg
    16561 agaatgtagt tatgctgaag ccagacgaat agctacaatc atcaaaaaaa tgaaatcgat
    16621 ttcgtagttg gcgttggcgg aggaaaactg accgatctag tgggttatag tgccaatctt
    16681 gcaagaatcg atttcggagt catccctact ttagctagta attgtgctcc atggacacct
    16741 cttgctgtca tgtataagga gaatggacaa gcagaaggga aaacagaaca cttttacaga
    16801 caagctgctt tttttataac agatccccaa ttagtgattg attcccctgt tccatatttc
    16861 attgccgggt tagcagatac actagcaaaa tggtatgaat ctgagctcat cttagaacaa
    16921 gattttttgc aagatgaatc atttttgaag ctagcacaag atactgcgaa gatctgcaaa
    16981 gaagagattt tgaatcattc tgctaaagca atccaagata tggcacaaag aaaactgaca
    17041 tcggagttca aacagctttc agagattatc tttgctgttg ctggtttagt tggaggatta
    17101 ggagataaat acgcaagaaa tgtggcagca catgctatgc atgacgctat cagtaaatat
    17161 attccggaaa gccatcgttt tctacatgga gaaaaagtag cgtatgggat tttctatcaa
    17221 ctggcactag aaggtcgctg ggaaaaaata gacgagttat atcctttgta tcgggaatta
    17281 gaactgccgg tttctctaga acaaatggga ttatttccga aagataaaca gataatagat
    17341 aaaatggctg aatttattga ttcgaaagag aaagtgcatt tgattcctat agaggtaaca
    17401 cagaaatcac tgatacagtc aatgatggaa ctggaaggtt atatcagaaa gatctgaagg
    17461 aggaggacac atggaaacat taagtgtagt acaaagtttg ttgattgctt tatgggtagc
    17521 cgcggttatg tcccgctggt taggtggagg agccacgttg accttacgtt tttctccttt
    17581 gatgactgga ttagtcgtcg ggttgatcat gggggaagtt gcacaagcga tgattgttac
    17641 agccgctttg caaatgattt atatgggggt tttttctcca ggaggatcaa tgccagctga
    17701 accatctatt gcagcagcta tcgcagtacc agtcgcatta ttaggggatc tgtcgccaga
    17761 agcagcgatt gccgttgcgg tgccagtcgg attgctcgga agttacttgt atcagtttcg
    17821 ttttttcatc aatacttttt taggtaaata tacagacaaa gctgtagaag atttgaatac
    17881 aaaagctatg gcccgatcaa ttattcttta tccaacggtt gcctcatttt tattattcgt
    17941 tccgcttgtt ttcattgctt tatatttagg ggcgccattg atcgctgatg taatcaaagc
    18001 tttagaggga acagtcgtta tccacattct tgaagtagta ggtggagggt tagcggcaat
    18061 cggtatcgcg acaactgtat acgttatcgg tcgaaaagat tatctggtct ttttcttcct
    18121 tgcttatttt atgagtgttg ttttgaaatc tttagatatt acgatggtta cttatgccat
    18181 ttttgggatt attatcgctt tgatctttgt ccagtcgcaa aaagggaaac aagtacagcc
    18241 tgccacaagc aataacaatt cagcatcttt tgatgacgaa gatgactatg atgacggatt
    18301 ttaatttagg aggaatggga aatggctgaa ataatggata atcaaacagg tgctcctgaa
    18361 gaaatcacga aaaaagatgt aacaaaagcc tatctgagat ggcattttgc aaacgaaatt
    18421 cctcactctt ttgaacgtta cttggcgcca tcactactat acggaatgat gccaatcttg
    18481 aaaaaacttt ataaagatga agactcttta aaagcagcat ataaacggca gttactgttc
    18541 ttcaacactc aattaagctg gggtggcggt gtgattactg gcctaatgtc ttctatggaa
    18601 cagcaaagag cagaagaaga gtatgaagaa aaagagatct tgatgcagga tgacttgatg
    18661 tataacacga aagcgggatt gatgggggca ctagcaggaa tcggagatgc aatcgattca
    18721 ggaaccgtcc aatatatttt tatcgcaatc gcggtacctt gggcacaaca gggaagtcct
    18781 ttaggcgcac tttttccttt tatctgcttt gggttgtatc aggttttact aggggtattt
    18841 ttcgcaagac aagctttcac tatgggccgt aatgctacag gcctaaagca aaacactggt
    18901 gttcagacag cgattgaaat gctatcagtt ttagggttgt ttatgatggg ggtgcttgcg
    18961 ggaaactatg taaaagtaga atcttcattg aaatttgcga tatccggacg tgagtttgtt
    19021 atccaggaaa ttcttgatca aatcgtgcca gggatattac ctttagctgt cgtaatgggt
    19081 gtgtacttgt tctatacgaa aaaaggattg aaagtcacac aagcattatt atggctgact
    19141 ggaatattaa ttgtgctagc aggaatcggt attttataaa aagaagggaa cgtgaataag
    19201 atgggtgtag tcaatttagc gcgtgtggat gagcgtttga tccatgggca agtaatggta
    19261 acgttatctc aaaaaagtgg agtgaattcc atttttgttg tagacgaagt tgtagcaaaa
    19321 gataaattta tgagagatct ttacaaaaat gcaggaagcc gtacaggaca aaaaacaatt
    19381 gtcattacac cagagaaagc taaattctat tgggatgaat atcaatttaa agaatacaat
    19441 tgtattttga tcgcaaaaac agtccaaacg atttacgact tagtaaaaca cggaatcccc
    19501 atggaagaat tgaatattgg agggattgct caaaaaaatc ctgatacaga tcttttagtg
    19561 acgaaatctg tttatttaaa taaatcagat gctgaaaaat tgaaagaaat gaatgaacaa
    19621 tatggcgtaa agaatattta ttttcaagcc actccatctg cacctaaaac aggattaaaa
    19681 gatgtattga acaagtttga tttgtcataa acaaatgctt agaaaaaaga taaagagggc
    19741 gaaaaaatga agaagaacga gctgtttcgt gattgggaat ttcgttatcg ctatatttac
    19801 cgtaagcgcc gtacaaaaaa gagtaaacag cgttttcttt cagcattagt atcagatatt
    19861 tacagtatga gaacagatgt gacagtcatt gcttacgata cgctagccta ccgatcaaaa
    19921 aatatttatg ttggagatat cgaaaaagca gagaaagtta tctgtactta ttacgataca
    19981 cccgttcatg cattaggaag ttatttcatg tttgattgga aagaccaaag gaaaaaaaca
    20041 atctactcta tattattgtc cttcattttg ttgttcagtt taggttggtg gggaatgatg
    20101 atatataaca aaaatcctca tcacgtattc gatctgctat ccgtataaac aggtatcact
    20161 gtactagcat ttgggagcta ctttttctta ttgggaaaag cagctcgcgg atggcctagc
    20221 agacaaactt ttattcgaaa tacttcatcc atattaacga tgctagaaat gatccgtacg
    20281 attgatgatc cgagtgtagc gtatgcgttt gttgatgaag gctgttatgg agaaaagggc
    20341 ttagacagcg tgagatcggg tatgaaaaaa gaggctattc ttttttatct agactctgtt
    20401 ggggcagata caccgctaca gttttcggga aattattttt caaataaaga acagtggctg
    20461 aaacaagttg ataagttgaa agaaaaaaac gtcaattata tttttagtgc aagaaaaaag
    20521 caagcgcagt tcttctattt gaccaaaaca gatttacgag ggaaaacatt taattggcaa
    20581 aatgccaatc agattattgc tctttttcga taagtaaaaa gtgcaaaaca aggagtgaga
    20641 agactaatgt tggaaatcat gatcgttaca catggtaagt tgagcgaagg actgaaagat
    20701 gcggcggaag taattgtcgg gacatcgagc catattgaaa caatgtgctt agagtctggg
    20761 gaggacatcc aaacattcgg agagaaggtc aaagaaaaat taactatgat caacacagca
    20821 gaacccatgc tagtatttgt tgatctaatt agcgcaagtc cgtacaatca agcgctcttg
    20881 gcagtcaatg aactgactga agaaaagaaa gcagccactt atgtcatagg gggcgtgaat
    20941 ctgccaatgt tgttagaagc aatcaatcat cagatgcttc acacaccgat agaagaagcg
    21001 gtggaacaaa ttgtcaaaca aggaaaagat aatttaactg tttggcatgc ttcagcggaa
    21061 gaaaaaaaat tagatgagga cgatttttag aaggaaggct gaaaagatga agtggggatt
    21121 tagatggtat ggtgaaaaag gggattcgat cccattaaaa tatgttcgcc aaataccggg
    21181 aataaatggg ataattggaa ctttgctgac taaaatgcct ggagatgttt gggagatttc
    21241 agaaattgag gagttgaaag catctgttga gagccaagga ctggaactgc ttggaattga
    21301 aagtgtagcg atccacgatg caatcaaggc agggacagat gaacgagatt actatattga
    21361 taactatata cgaacaatca aaaacttgtc tcgatgtggg gttcgattga tttgttatag
    21421 cttcaaacca atttttggat gggcgaaaac tgatttgttt aatgaaaaca acgatggaag
    21481 ctattctctt ttatacgatc agaaaatcgt cgatcagatg gagccaggag aaatgtatca
    21541 attgatacac agccagtcaa agggattcaa attgccgggt tgggaagaag aacggttgaa
    21601 aaaattcgct cagctggaaa aactgtatga aggaatgacc gaagaagaat tatttgaaaa
    21661 tctgacttat tttttagaaa aagtgattcc agtgtgcgaa aaagcagatg taaaaatggc
    21721 tatccatcca gatgatccgc catgggaact ttttggtttt cctagagtga cgaaaaatct
    21781 tggcgacttg aagaaaattc taaatgcagt agattctcca tataatggac taacgctgtg
    21841 cacaggatct ttaggggcaa atccggaaaa cgatttagtt gaaatcatcc atgaggtagg
    21901 ttcgcgcatc cattttgtcc acttcagaaa cgtggagttt ttaggagaaa gaaagttcaa
    21961 agaaacggca catccctcta cagaaggctc ccttgatatg tatcatatta tgaaagcgtt
    22021 ggtgcaagaa gggttcgacg gtcctatacg ccctgatcat ggtagaaccg tgtggggtga
    22081 agtggctatg cctggatatg gcctgtatga tcgtgcaatg ggactcactt acatgcaagg
    22141 tctatatgag gcgataagta aggaaaaacc actgggagag cattatgaat aaaatcaagc
    22201 tcttcatctt tgatatggat ggccttttat tagaaactgg acgtttagct tatcgtgctt
    22261 atgtaaaatc agcacaaaag tatgattatg aaatgcgtaa ggaagtctat tatttactaa
    22321 ctggccaaac agaaatggcg ataagagaac aaatgggcat tttgtacgga gaagatgttc
    22381 catatatcaa atggcgagaa gcaatcaatc aatataaaga aaaaatcgtg aaagaagata
    22441 agagagtata tacgaaaaaa ggagcagaag aaattttatc ttttgcaaaa gaaagaggaa
    22501 tccatacaat cgtggcttcg tctaatacac gggtaaaagt agaaatgtat ttaaaaatgg
    22561 aaaatctata tgccttgttt gatcaaatca tttcagggga tgatgtaaaa aaaggaaagc
    22621 cggaaccaga gatctttttg aaagcttgtt caaaaatgaa tattcctcca agtgaagcac
    22681 ttgtatttga agactcgatt gcagggatcg aagcagcaag gcgagcgggc attttgtctt
    22741 ttctcatcaa agatcatttg atagggttgc ccgatcataa tgggaagcat aagttgaaag
    22801 tagatgtaag tttatatgaa tccaataaag cagactatga attttatgac ctgcatcaag
    22861 caaaacgatt tttagaaagt aaagggttgt atctctaata aagaggacta ttttctagtc
    22921 ctctttattt attgtcctgc acgactgagg gaacagatga aaaagaagta attttttatt
    22981 ttgaagtatc taccttattt gtagtgaaca atttagaaca aacacaaaac gcatctactg
    23041 ttatctacca gtagatgcgt ttaaagagaa gaacgagaat cgtccatatg agggtttcca
    23101 ctccttatgc agattttttt aattcttgca ctgcttgttc gatcgtaaca cctgtcgcta
    23161 catgattggt attttctttg tcaaaaacga caaatagatt caaatctgta tccaaggtta
    23221 ctcgatattg tatatctttg ttatagaatg tcatagccat tctcctttct ttaaatactt
    23281 gctgaaaggc aacgaacgcg ttgataacga aattccattt ataagtatac caccaattga
    23341 aaggttttca tagttaagaa attttaaagt caaaagttgc agataaaagc aaataaacaa
    23401 gaaaacaaaa agtgaatcaa tcgcggttat tcaacttttt cttcgattcg aagacaatag
    23461 aagacaatga attttatatt tcattgcatt atccttgtgt tattagaaaa agcatgatat
    23521 attaaatgag taatctattt tgaaacgtaa tttgagcgat atatctacat agataaaact
    23581 ccttttgcct atcaaatatt tattgcaacg aaaaaacgta cattgatacg tatcaggagg
    23641 aatttattta tgaataacgg tacagtaaaa tggttcaacg cagacaaagg ttttggcttt
    23701 atcactggtg aagatggaaa tgatgtattt gcacattttt cagctatcca aggtgacggc
    23761 ttcaaaactt tagaagaagg ccaagcagtg tcttacgatg ttgaagatgg tcaacgtggc
    23821 cctcaagcaa caaacattgt taaagcttaa taaaaattga cttttaaccg tcttttcgaa
    23881 agaaaagacg gtttttttgt acgaaaaaat tcattggtcg ttgaatgggc tgaatagtag
    23941 tgtataaaac aattttgtgg agcgtgaagc ttttatggta gaggaaaata cgaataaagt
    24001 gatgttgata aaaagattcc tatcttgaat atgaatcgag atgtcttgaa aatgaatgag
    24061 tttgaccaat cattatccta tcgaaattta cagtttcgtt cgattggtct gtcaattaat
    24121 gaaaaagtaa aaaagaatat gttctaggaa gagggcggca catctaaatt tttaagtaac
    24181 ggacagttca aatatcaagc attgaaagag gctatcctac tgtttgaagg aatacgaata
    24241 tataaaaacg acaaagagga aaaagtagtt cattaaatcc cttcaagtga tgaaagaatg
    24301 agatctaata aaacctgatc tttcatctgt ttttttaata acgtaataat ttttttattt
    24361 tttgtgcttt cgccgttatt gtagagtgat ttagcattct tatttttaaa aataactgtg
    24421 ttatatatta aaaaaggcat aatagttata agctttttta aagaatattt agaaattatt
    24481 aattttcgtc tgaaaacaaa aaaagaaagc gttattattt tagtctattt tcaggggggg
    24541 aaccttatga taaaaaaact atttttacta tcaatattaa cgctagcaat ggcaggttgt
    24601 tctgactctg gcttagcaac tgaactgatc acaaaatcaa ctgaagccac gacagaggag
    24661 ttaaaatgtg gaaacgatca cagtgactac agaggcttaa atcgacgatg catgggaata
    24721 ttaggcagaa gcaattgtaa gcatcgatgt agtaatcgaa gaaggtgtag aagaagcaac
    24781 agaaaaatca aaagaaagat caaaagaagc aatctgtgct gtgcatcgta tacagacgtc
    24841 gaagatgcgg tacttttaaa ccacgtcagg agatgttcat ttatgtctag ggaagttggc
    24901 gatgaaccaa aactagagta cgcttttagt tttgcacgtg aggatgaaga taaccctaaa
    24961 acttacgaca ttttagcttg atctaagatg gttaaagctt tttaatgaag taagaggaga
    25021 tagaaggctt ttctagttat attaggtaag ataaattcct ttttgctcca aatttgctaa
    25081 acattctgct agataaactt catcgtgttc cggataaatc ggcacgttgc gctctacagt
    25141 aggtaaatca aaaaatgctt cgcatgattt gccacgatac ataggatttt tccactctgg
    25201 tgctgggctg taaccacgtt tttccatttc atccatgaca agctgatgat attggaataa
    25261 tctataagga gaatagtgaa aaacataatc cacagtagca tgtttctttc cccagccatt
    25321 tcctcttaat gcacagcatt cccgatgttg tcctaaaagc tgttgacgag gaagctttga
    25381 aatcaatttt tcatgccata atcgcataga aatgtctcct ttatatatag attgttaacc
    25441 ttagacgtta aatgaaaaaa ccagcaccaa ggctggaaaa aatcacttca gtttgcttgc
    25501 tgtttctgag cgaccaatta ggtaatcaac ggaaatatcg taaaagtcag cgatatagat
    25561 taattcaatg atgcttggaa tttcttctcc ttcttcgtac ttacgatatt tgaataaagg
    25621 aatacttaac gcgctagaaa cagctacttg agataaagat cggccagttc ttaagaaatt
    25681 cagacgacaa cgtaaagtat ccagtaaaat agcttcttcg atactcattt aatcaccttt
    25741 cattatagta gaatagttat aatgactata gaaagctaca catgcaatca atagtactca
    25801 atgtttttta tttaaacgta tcaaataaag aatgacgatc aataaagaca aaaccagtcc
    25861 tgataattct tctataaaaa cactatcggg ttcaaagtag ttgtaattaa aaactttcca
    25921 cataaagtag atagccggaa gagtcaaaat agctaaataa aaccgttctt tgttccaatt
    25981 cataagataa acaatacttg cgaccagtgc gaccgcaggc acccaaaaca aaactctcac
    26041 caacgtaatc atgtaacgga ctcctcaacg attcatataa taaaagataa catgttattg
    26101 catacatcac aaatgaaatg ctaggaaaat aaaagaagaa tagatcaatg aaaaatgaaa
    26161 aagcagaaca gttttctgct gataagcagg aactgttccg ctgaatgtgt cttatctatc
    26221 gagaaaaaat caatggattg atatctaagt aattcaatac atataaaaag aggacgatcg
    26281 cattcactaa aaaggaaaga atgatgatcc catagcgaat ggaaacatat ttaacaaata
    26341 cttttgtttg aatacgggca tatctttttc ctttatagct gagcatcatg acaagaagca
    26401 aaagggtaat cacaccttga aaaatcattc caataaacag aattcctgag gtttgataat
    26461 ttaattggag aaaataagct gctgccgcaa ctaaatcgaa taaaatcgca ataaatggca
    26521 aaataatcac tcctaacatt gagaataaat aaaagaataa cattttttct aaaataagca
    26581 aataaaaatt aa
//
LOCUS       DAJSAW010000034        25760 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJSAW010000034 DAJSAW010000000
VERSION     DAJSAW010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.034"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            223..777
                     /locus_tag="ONT64_001660"
     CDS             223..777
                     /locus_tag="ONT64_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2303080.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(950..1423)
                     /locus_tag="ONT64_001661"
     CDS             complement(950..1423)
                     /locus_tag="ONT64_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2303081.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1435..1971
                     /locus_tag="ONT64_001662"
     CDS             1435..1971
                     /locus_tag="ONT64_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2303082.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            2011..2382
                     /locus_tag="ONT64_001663"
     CDS             2011..2382
                     /locus_tag="ONT64_001663"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2303083.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2666..3937
                     /locus_tag="ONT64_001664"
     CDS             2666..3937
                     /locus_tag="ONT64_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR2303084.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4182..5207
                     /locus_tag="ONT64_001665"
     CDS             4182..5207
                     /locus_tag="ONT64_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2303085.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5501..6610
                     /locus_tag="ONT64_001666"
     CDS             5501..6610
                     /locus_tag="ONT64_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR2303086.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6693..7589
                     /locus_tag="ONT64_001667"
                     /pseudo
     CDS             6693..7589
                     /locus_tag="ONT64_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7605..8429
                     /locus_tag="ONT64_001668"
     CDS             7605..8429
                     /locus_tag="ONT64_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2303087.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8461..9771
                     /locus_tag="ONT64_001669"
     CDS             8461..9771
                     /locus_tag="ONT64_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HCR2303088.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9919..11178
                     /locus_tag="ONT64_001670"
     CDS             9919..11178
                     /locus_tag="ONT64_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR2303089.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEYRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11188..12060
                     /locus_tag="ONT64_001671"
     CDS             11188..12060
                     /locus_tag="ONT64_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2303090.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12072..12902
                     /locus_tag="ONT64_001672"
     CDS             12072..12902
                     /locus_tag="ONT64_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2303091.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12942..15125
                     /locus_tag="ONT64_001673"
     CDS             12942..15125
                     /locus_tag="ONT64_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR2303092.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15186..16406
                     /locus_tag="ONT64_001674"
     CDS             15186..16406
                     /locus_tag="ONT64_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HCR2303093.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16403..17863
                     /gene="gtfA"
                     /locus_tag="ONT64_001675"
     CDS             16403..17863
                     /gene="gtfA"
                     /locus_tag="ONT64_001675"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR2303094.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17940..19046
                     /locus_tag="ONT64_001676"
                     /pseudo
     CDS             17940..19046
                     /locus_tag="ONT64_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19368..19577
                     /locus_tag="ONT64_001677"
     CDS             19368..19577
                     /locus_tag="ONT64_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCR2303095.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19570..19719
                     /locus_tag="ONT64_001678"
     CDS             19570..19719
                     /locus_tag="ONT64_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR2303096.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19873..20538
                     /locus_tag="ONT64_001679"
     CDS             19873..20538
                     /locus_tag="ONT64_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2303097.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20822..21907)
                     /gene="xerS"
                     /locus_tag="ONT64_001680"
     CDS             complement(20822..21907)
                     /gene="xerS"
                     /locus_tag="ONT64_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HCR2303098.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22241..22474
                     /locus_tag="ONT64_001681"
     CDS             22241..22474
                     /locus_tag="ONT64_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HCR2303099.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22471..22878
                     /locus_tag="ONT64_001682"
     CDS             22471..22878
                     /locus_tag="ONT64_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCR2303100.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(24015..24533)
                     /locus_tag="ONT64_001683"
     CDS             complement(24015..24533)
                     /locus_tag="ONT64_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HCR2303101.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            25651..>25760
                     /locus_tag="ONT64_001684"
     CDS             25651..>25760
                     /locus_tag="ONT64_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2303102.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT         8784 a         3935 c         4801 g         8240 t
ORIGIN      
        1 cccgtctaaa atttaaataa gagcttagaa tcttcatata agtaaggtta atttttttgt
       61 acaaaaaagt cggaatattc tgagttactg aaatgatata aaatcataaa ctaaatccct
      121 tgattttatt gactttcaaa agtttatata tataagttaa tgattatggt aaaatatata
      181 cataatcatt aactcagaat atttttaaga agggaaacaa tagtgagcta taatgtacaa
      241 ccgttaagga caaatgaaga gatacaagat tttcagtttt ggctaagaag aacaagtaat
      301 ccggaaagag atagtttttt atttttgttt ggaatcaata atgggttacg tatgtcagat
      361 attataggat taaaagttgg agatatacga ggcaaaagta agcctattat tgttgaacat
      421 aaaacaggta agcgaaaacc gatttttata gataatttga gagaagaaat tttattatat
      481 actgaaggta aagaagaaaa tgattggtta tttccgagtc ggcaacaagg tcgacatata
      541 acaagggatc gtgtctatca gatatatgca gatatagcag aaaaattagg acgagatgat
      601 ataggaacac atacattaag aaaaactttt gggtatcact actacaagaa aactagagac
      661 attgctacct taatgtttat ttttaatcat agctcgcaag caattacaaa aaggtacatt
      721 ggtataactg aagatgaaat aggagcatca cttcgtggat ttaaactagg tgtataattt
      781 aaaaattttt ataaacagta aagacaaaga ttaaaagcta tttttatcta gaacgatttt
      841 atttcataaa gttcgcggaa taaaagctgg ggttactggt acttggtata ggttcgtgtt
      901 atataaacta agtaattaaa taacattttt aggttggaaa aggatgaaat taaaaaaaga
      961 gaatcaatac ggtctcaaaa acgcaaacgc tgcggaataa gaaaaatact gataactcaa
     1021 cgtttagcag actatctaat agggatagtc tgcttttttc gtttttgacc atacttttga
     1081 ccttctgcaa acttccttgc ttttcctctg tagaactcat gaaatctgca aaacgaattg
     1141 ccgtttcctc tgtctgcttt ttcgcgatat gggtgtatat atcatccata gtggttttaa
     1201 tatctgtatg tccaagtcga acatacactt ctttgacaga cgcaccagat tcaaagagca
     1261 gactacaatg ggtatgacga aaaccatgaa gtgtgatatg ttttaagtta ttctctttaa
     1321 tgatgatttt caaattgtga tttacatagt ctaaataaag gggcttattt tcttctgatg
     1381 aaaacaaaag ttgttcacca tcttttatag gtttgattct catttttaga tattatgccc
     1441 gcacgtattc atcacatttc tattgtgaat cgctttattc gaccgacatt tgacttttat
     1501 cataatatac tcggtttaaa attactaatg aaaacaatta atcaagacga tcataccatg
     1561 tatcacctgt ttttttcaga taatcaccac cgtgtgggaa ctgaactgac tttttttgaa
     1621 gtacaagaag ggaacaacca gttttttgga accaatacga ttgaacgaac gatactaaaa
     1681 gttccttccg aagcatcatt gcatttttgg gaaatatatt ttgaaacaca aggcgtttgc
     1741 cattatggaa tcgaatcatt ttctggacga cctatcttac gttttgaagg gccagatgca
     1801 acacgattag ctttagttcc cttacgcgaa tttgaagata ttgatgatta tttcccgtat
     1861 gtcacagacc agatccctac agagcatgcc attttaggaa ttgatgccat tcaactacgc
     1921 gtacaatatt ccgatgcgac tgaacgagag ttattgtcgg taggctggta gcacaaagaa
     1981 tacgtcagtt tcttttcggg tcatcaacga gtgacaatct tagaaaatca agacactcag
     2041 ttctatcaag aagttcagat tattcaagat cgtgagaatc cagttgccat tgaagggatt
     2101 ggtagcgtcc atcatgttgc gtttggcgta gaaaataaat ctgatttaca acgcatcgac
     2161 aaacaattac aagagcgcaa ttttataaat tcaggaataa aagatcgtga attttttgta
     2221 tcactttatt atcgtgaccc aaatcaatta ttgattgaaa ttgctacagg ggaaggaaac
     2281 ctagatgcaa aagcgtatga aaatcaaagt ccacgctttg aagaaattcc tttattttta
     2341 ccgcagtact taaatttcat tcgtgaacaa gtggagcaat aattagctaa tcaaatccat
     2401 gacgaagagt aagaggttga ctcataagtc aaaaaataaa gaaaaacgca tcaaatccat
     2461 tctaaagaat gttgatttga tgcggttcct atttttataa actatcattt tcgactaata
     2521 aaactactta tgagtcagcc tctctttttt tcttgatttt tatacctctt tctaggtaaa
     2581 ctaaacatct gccaaaaata ccgtgtctca ttgaaacatt ctcaactatt tcaaaataaa
     2641 ggggagtacc aaatgaacga gtatattgaa attatccacg caagagaaac aacttaaaaa
     2701 tgtcagtctc aaaattccca aaaataaaat tagtattttt accagcgtat ctggttcagg
     2761 aaaatcatcg attgtttttg atactattac ccaagaagct ggtcgtcaac tgaatgacac
     2821 ctatgatagt tttacccgtt tatttttacc taaatacagt cgtccagagg ttgatgagat
     2881 tgataatctt tctacggcga ttgtggtcaa tcaaaagaag attggtggca gtgcgcgctc
     2941 aacgttaggg acgattacta acatcaatgc gttatttcgt gtgttgtttt catgttttgc
     3001 aacacctagt ttaggatatg caaatgctta ttcatttaat gatcctagtg gaatgtgttc
     3061 tgtctgtcag ggaatcggta agacgattgc atttttaata ggattgcttt atttttcggc
     3121 tattgataat gctgattttg tattttctga tccatttacc gcagaagttt tgtctggaat
     3181 tgaggaacag gcaaagaatc atggttattt tgtattggtg cataatgtta attcagcaga
     3241 agatgttaaa actattcaga gaaattggcg ttttgagggg ttcattgttg ttggtgtacg
     3301 tgtaaaagaa tttttggaat taaataacgt ttttgatggt cctgttgttt atattgatac
     3361 gcatctttca aaagagacat taagagaact tgaacaacaa atgaatcgtt tatttgtaaa
     3421 tacaaatgat taccaagcta gtacattggc agtggaacat ttgattcatt atggtcatga
     3481 gaaaatagcg tttctttctt ttgactacat tgtaggtgaa ccaagtgtta ttcaagaacg
     3541 acatcaagct tatatcgata cattaaagaa atatcattta acctttaaat cgcattggat
     3601 atataacaac tctgaatttg agcgaattta tcaagaatta gctgaattta ctgcaattgt
     3661 tgttactgga gattatttag cggctaaatt agtaaaattt ttaaaaaata agaataaacg
     3721 agtgatggaa caattatcga ttattggatt tgatgatatt acttttgcag aattaatgga
     3781 tcctgcgttg acaacaatac gactaaatcc aggtaacaag ggggcgattg cttttcacca
     3841 actgattgga ctggccaaac aagaagaaat aaaaaatcaa gtactactat tagagggaga
     3901 attgatttct cgagactctg ttcacaaaat caaataaaaa agctctgtga aataagtata
     3961 gttaacaata aaatatccga acgatagaga gaatgaactg tgatctagca gttcctcgtc
     4021 gcaaattcac tagaattagc attatttact ctaaattgtt cggatatttt ttcttgtttt
     4081 taattatgat tacagtgcct atttttaacg aaatttgctt attttcaaaa tgtatgatta
     4141 tatgttagta aacactttca aatatgaaaa gaggagctat tatggtagga attagggatg
     4201 ttgcaaaacg ggcgggtgtg tccattgcaa cagtatcgcg cgtgctaaat gaggatccga
     4261 cactttcagt aaccgatgaa accaaacgaa agattgcaga atcggttaat ttttatcatt
     4321 atgtcaaaaa aagtcccgtt gcaaaaaatt taacaaatat tgcattaata acgacagttt
     4381 ctgaagtaga tgagttggaa gatccctatt ttcgaacgat tcgcagaggg attcaagtcg
     4441 aagcagaaca aagcaaagtc aatttgaaga aaattattcg tttgtcagaa acaccgttgg
     4501 agcaagacga tttgctaaaa tgtcaaggtg tgttgattat tgggcaggtg ctatcttctg
     4561 tgattgaaga agtatcagca attaattcga atgtcgtagt gattgatgac cctagtattg
     4621 aagctgaagt agatgcagtt tatactgatt taacaaaagc aacgaatcag catttagatc
     4681 gattgtataa aaaaggacac cgaaacattg cgtttatcgg aggaggacgt gttcgtttag
     4741 atagccaagg aaataaacac gtgtcagatg atgattatcg aaaaatagcc tatgagacgt
     4801 ggatgaaaca aaaaggatta gaaaaatata gtcaaattta tttagaaggc tggaccacac
     4861 ttgacggaat gcaggcagca gatttacttt tgaaaaaaca tgctaaagat aagttgccga
     4921 ctgctatcat ggtaggaaat gatcctattg ctgtgggggt ttatcgttca cttcaaaaaa
     4981 atggtctaca tattccagaa gatatttcga ttgtgagttt tgataatatt gaagttgctg
     5041 aatttttaac acctgcatta aatacggtga atgtgaatac agaagaaatt ggaaaactag
     5101 cagttcgcat ggttaatgaa cgaataaaag aagttagaaa tgtttcgatc aaagtgattg
     5161 tttctaataa tattattgtt agagaaagtg aaaaaaatat cgattaaaaa agtaaacatc
     5221 caaaaaaggg tgtttacttt tttgtttact aatattttta gtaaaaaatc tcgacaaaac
     5281 attaagtaaa cagttatggt gttgttaaca aaaagaaaac gcattcaaaa caggaggaat
     5341 taagatgaaa catttcaaaa aaatagcttt aacagggata gctgttttaa ctgggacatt
     5401 actcgcagca tgtggtgggg gagatagtaa ttctaaagac gggcaagtaa ctttaagcta
     5461 tgcgagttga gatactgacc aagctccagg actaagaaaa atgttagatg aatttgaaaa
     5521 acaaaatcct gatatcaaag tagaaatgga gacaacgcct tgggatcaat attgggttaa
     5581 attagaagct gcaacaacgg gaggtaatat gccagatgta gtaaccatgc actctagcga
     5641 atcctataaa tatatgtcac agaatgcttt gatgggactt gatgatgtta tcaaagaaaa
     5701 tgatattgac atgggaaact ttactgaagg aattgctgat ttctatacgc ttgaagacga
     5761 actatacgca atccctaaag atgcttctgt agtggggcta tggtacaaca aagaattatt
     5821 tgataatgca ggtgtcgatt atccagatga aacatggaca tgggaaacac tcagacaagc
     5881 tgcaattgat ttaacagatg aatcaaaagg catctatggt tttgcatcac atattggaac
     5941 agagactggc tactggccat ttatttatca aaatggggga gaagtatttg ccgatgacaa
     6001 tacaaaatca ggtttgaata ccccagaagc acaagaagct ttagcttttt atcgagatat
     6061 tgcacttgtt gatcatgctt caccaacaat tacagaatta caagatcctg ataagttaag
     6121 tcgttttcaa tcaggtagat tagcaatgat gattgaagga aactggtttg caccgggatt
     6181 tttgaaaaat gactatctag ttgaaaatgt tgcttctgcc gtgttaccta agggaaaagt
     6241 taatgcaaca attacaaatg gacttggttg ggctgccagt gcgagtacaa aatacccaga
     6301 agaagtgaaa aaactaatgg catttttagc tagtgaggac gccaataaaa tccaagcgga
     6361 aacgggggca tcaatcccag cactaaaagg ctttgaagac gagtgggctg cttcttcaga
     6421 tacaattgat ttgagtgctt ttggaaaaat gttggagtat ggagtccctc gtccatacaa
     6481 taaagatgga ttacgagctg aacaagcaga agttcaaatt attaaccgta ttctgtctgg
     6541 tgaagtaact gttgaagatg gaaccgctca aattgaaaaa gaagtcaatg cgatattgag
     6601 cggaaattaa attagttaat tatacaatat acaggcgaaa ggattctatt tcgtctgtat
     6661 attaatggat gaataccaaa aagggggata ttatgaaaaa tcgaattttt ggaaaatatc
     6721 gtaaagatca agtgatatgg ggctatattt ttatttttcc aacggcatta ggattaattt
     6781 tattaaatat atggccagca attcaaacag caattttaag ttttcaaaaa acggtagggt
     6841 tcggtgatac tgaatgggtc ggattaagaa attaccaaaa aatgtttgca gacggtgaag
     6901 tttttcagtc tttattaaac acattaattt atgcagtggt ttcgatacct agtattgttg
     6961 tattatcctt gttggtggca gttttaatga atagaaaagt taaaggattg tcgatttatc
     7021 ggacaattta ttttctacca gtcgttgctg caccatccgc agtcgcaatg atttggcgct
     7081 ggatgttcaa taatgattat ggactgatta ataacatgtt ggctaaaata ggtctcgatg
     7141 gaccagcatg gttaacggat ccatcgattg cgatttattc tattatcatt gtaggagttt
     7201 ggagtgctat tggttataac atggtgttat tactagccgg cttataagaa attcctaaag
     7261 attattatga agcagcggaa attgatggtg ctagtccaat ccagcagttt ttcaacatta
     7321 cgttaccatt agttacacca acattatttt ttgtagttgt aacaacagtt atcaatgctt
     7381 tccaagtatt tgatgttatt tttatgatga ttactcctaa tagtaccgcc atgtttaaat
     7441 cacaatcatt agcctattta ttctataaac attcatttat tttaaatgac aagggatatg
     7501 gttcggcaat cgttatgttc cttttagtga ttatattaat tattactgca attcaaatga
     7561 agttgcagaa aaagtgggta aactattaag gaggctatca aattatgaaa aataaatttt
     7621 tttcaaatgc gccaatccat atcatgctta ttttaggtgg tattgcaatg gcattacctt
     7681 ttatttggat gattttaact tcagggaaaa cgttggttga atctacccaa attccaccta
     7741 caattttccc tgagaaattt cagctaagta actatcaaca agtacttgat ttattaccat
     7801 tcatggcttt ttatatcaat acatttttaa tggttgttgg acgagtgatt ggctcggttt
     7861 tattttcagc aatggctgca tatgcttgtg cgcgtctaag ttttccaggg aaaaatatcc
     7921 ttttcagctt agtacttatt caaatgatga ttcccggttc catttttatt actccacagt
     7981 atttattagc acaaaaattg ggtattttaa atacaattcc agcattaata ttaccagggg
     8041 tagttagtgc gtttggtacg tttttattaa gacaattttt cttaggttta cctgatgaat
     8101 tggaggaagc ggcctattta gaaggtgcca atatttggac gatctttttc aaaattatgt
     8161 tgccgcttgc taaatcagga ttggttgcac ttggaatatt cacagcgttg tttgcattta
     8221 aagatttaat gtggccattg attgtcaata cttcaattga taagatgcct ttagctccgg
     8281 gattagcaaa cttacaagga caatatgcca cgaacttccc acaattaatg gcaggttcgt
     8341 tattagcaat ttggccaata gttgtgatat tcatcgtttt ccaaagaaaa ttcattgaag
     8401 gaattgctac aacaggtagt aaattataaa actattaatt agtgattgga gagtaagtac
     8461 atgacaaaaa ttacatttat tggtgcagga agtactattt ttgcaaaaaa cgtattaggc
     8521 gatgcgatgt taactccaag tctacaagat gcacatattg cattgtacga tattgatgaa
     8581 aaacgactaa aagaatcaaa tttaatgctt aaaaatatta ataaaaattc gaatgaaaat
     8641 cgtgctgata ttgtttctta ttatgatcgc aaagaagcgt taagagaggc tgattttgtt
     8701 atcaatgcta ttcaagtggg tggttataaa ccttcaacag tgattgattt tgatattcct
     8761 aaaaaatatg ggttgcgaca aactattggt gacaccatgg ggattggtgg gatttttaga
     8821 accctacgga cactaccagt aatgttagac tttgctaaag aaatggaaga agtttgtcca
     8881 aatgcttggt tattgaacta tacaaatccg atggcgatgt taaccatggg tgttttaaaa
     8941 gcgacgagta ttaagactgt tggtttatgt catagcgttc aagtatgcgt accagaattg
     9001 tttgagcatt taggaatagc cgatcaatat gacttaaatg actttcaatg gaaaattgca
     9061 ggcattaatc atatggcatt cttgttggaa attacaaaaa atggggaaga tttctatcca
     9121 acaattcgtc agttagctgc agaaaaacca aatccacatc cagattctgt acgttttgaa
     9181 ttgttgaaac gttttggtta ctatattaca gaatcaagtg aacacaatgc agaataccat
     9241 ccatacttta ttaaatcaaa atacccagaa ttgattgacg aattagggat tccgttggat
     9301 gaatacttac gtcgttgtga acaacaaatt aatgattggg aaagcatgcg agaggacatt
     9361 gtaaataatg gggagttaac acatacgaga agccgtgaat atgcttcata tatcatggat
     9421 tctattacga caggaactcc gtcagtgatt gcaggaaatg tcttaaataa aggcttaatc
     9481 acaaacttac cgacagatgt ttgtgtggaa gtaccttgtt tagtcgataa aaatggcgtg
     9541 caaccaacct atgtcggtga attgccaaca caattagctg ctttaaatcg gacgaatatc
     9601 aatgttcaag aactgacagt agaagccgct ttaacactag ataaatcaaa aatttatcaa
     9661 gcagcgtatt tagatccaca tctatcatct gaattgtctt taacagatat caaaaattta
     9721 gtggatgatt taatcgaagc acatggggac tatttacctg aatataatta aaaagaagct
     9781 gaaagagtaa ggaattttca attccttact cttttcttag ttaaatttag taaaaatatt
     9841 gacaaaacac ttagtaaaca gttacattag tgttaacaaa aagaaaacgc attcaaattg
     9901 tataaggagg agcataaaat gaagtggtct aaaaaaatta ttttagcttg tatagcacta
     9961 ggagcaacga tctctttgac tgcttgtgga aaaaatcagg aagatgcaag cggtaaggta
    10021 gaaatagagt tttttaatca aaaaaaagaa atgactcaaa cgattcagga gattgctaaa
    10081 gattttgaag caaaaaaccc tgatattcat gtaaaagttg tggatgtacc gaatgctggt
    10141 gaagtaatta aaacacgtat gcttgcaggc gatgtaccag atgtcattaa tctttatcct
    10201 caaagtatag aattgaaaga atgggcgaaa gctggttatt tagaggactt aaccgaagaa
    10261 ccgtatttag agaatatcaa aaatggatat gcacaacgtt ttgctattga gtatcgtgta
    10321 tatagcatac cattaaccgc taatgtctat ggtttttact ataacaaaac tgcttttgag
    10381 gaaatgggga ttcaagcacc agaaacttgg gcagaatttg aaaaaatagt agctgagatt
    10441 aaagatcaaa acaaagtacc gtttgcgatt gcaggagcag aaggctggac tttaaatggt
    10501 tatcaccaac tagctatagc gactgctgct ggtggtgaag aggaagcgaa caatgtttgg
    10561 cgtttctcag aagttaatgg aattaatgct gaaagcaaag aaatgcaaaa agattttaat
    10621 cgattagatt tgttaagaga accaaaagct ttgcaaaaca actggcaagg tgccggttat
    10681 aacgatacag tagttacttt tacaaaaggt gatgcgttga ttatgccgaa tggttcatgg
    10741 gcaatgccga tgattcattc acaaaatcca gattttgaag taggaacttt tccttttcca
    10801 gcagatgaag caggtcaaag tctaacgatt ggcgcaggag acctagcctt atcagtttct
    10861 gcaacttctc aacataaaga agaagccaaa cgttttgtag aatatatgac aacacctgaa
    10921 gcgatgcaga aatattatga tgtagatggt gcaccgtgtg ctgttgaggg cgttatcgag
    10981 aatactcagg attcaccatt aagtggttta actgaacttg cttttactga tcgtcattta
    11041 gtttggttag ccaaagattg gaatagtgaa aatgattttt ataccttaac aacaaattat
    11101 ttacacaatg gcaatcaaaa agccatgatt aacgcattaa atgctttctt taatccaatg
    11161 aaagctgacg gaaggtagga aaataaaatg aaaataaaag aaattttaaa tcgttactgg
    11221 gcaatcattt ttgtagcaat tccaattatt ttgcagatga tttttttcta ctttccaatg
    11281 gttcaaggtg ctttttacag tttaactaat tggactggtc tgacttataa ttttgatttt
    11341 gtgggtttga acaattataa gattcttttt tctgatccga agtttatcaa atcgattggc
    11401 ttcactttaa ttgtgacgct ttgcatgatt gctggacaaa ttggttttgg tattttgatt
    11461 gcgcgggcgt tgaacgcaaa tattaaaggg aaaacattct ttagagcatg gttctttttt
    11521 ccagcggtct tatcgggcgt ggttgtttct ttagtattta aacaaatttt taactatggt
    11581 ttaccaaaaa ttggtagtat gttaggcatt gaatttttac aaaccagtct tttaggaaca
    11641 acatggggag cgatttttgc agtaattttt gttttacttt ggcaaggaat tgccatgcca
    11701 gtcatcattt tcttagcggg ttttcaaagt attccacaag aaattattga agctgctaga
    11761 atggatgggg cgaatgataa gcaaattttt tggaatattg aaatcaccta cttattacca
    11821 agtatttcaa tggtatttat tatggcatta aaaggtggct taacagcatt tgatcaaatt
    11881 tttgctttaa ctggtggtgg accaagcgat gccacaacat ctttggggtt acttgtttat
    11941 aattatgctt ttaaaaacaa tcagtttggt tatgcgaatg ctattgcttt agtcttattt
    12001 atcttaattg ggattgtttc actggcacaa ttaaaattat cgaaaaaatt tgaaatctag
    12061 gaggtttccc aatgaaaaca tcaaaagaaa aaaatatttc taaatacttg cttttattat
    12121 tagggggctt catgatttta gttcccttag tttcaacgct attcggttca tttaaaacaa
    12181 cacccgatat tatgaataat ttctttcgtt ttcctaatcc agcgactttg gaaaatttcc
    12241 gaagattgtt agcggatgga attggtcatt acttttggaa ttcggcaatc atcacaatca
    12301 ttgcagtagt tgctgtgaca gcaattattc ctatggctgc ttattcacta gcaagaaaaa
    12361 tgactcgtgg gaaagcatac ggattgatgt atttattttt aattttagga atttttgttc
    12421 ctttccaagt aattatgatt ccgattaccg ttatgatgag taaaattggt ttagcaaata
    12481 tgtggggatt aatcttactc tatctcactt atgccgtacc acagacacta tttttatatg
    12541 ttggttttat taaactaaat gtgcctatca gtctggatga ggctgctgaa attgatgggg
    12601 ctagtcactt tacaacttat ttcaaaattg ttttcccaat gttaaaacca atgcatgcga
    12661 cgactattat tcttaatgct ctatggtttt ggaatgattt catgctacca ttattaattt
    12721 taaatcgtga ttctaatatg tggacactac cattgtttca atataattat acgggacaat
    12781 attttaacga ctatggtcca agttttgctt cctatacagt aggaattgtc acgattacac
    12841 tagtttatct tgctttccaa agaagtatta tagctggaat gagcaatgga gcagtgaaat
    12901 aaccaataaa ttattctcat tataaaggag ttcataaact tatgattata tttgacaatc
    12961 acacacaaac atttcattta caaaataaaa aaattagcta tctattagga atcgaagaaa
    13021 ataattattt aacgcatctt tattttggaa aaaagattac gcactattct ggtgggtatc
    13081 gctacccccg aacagatcgt tccttctcac ccaacaatgc aaatgcgcaa gatcgtttat
    13141 tatctttaga cacattacca ttagaatttc caggatatgg acatggtgat tttagagaac
    13201 cggcgcataa tattaaatta ggaaacggta gtcgtgtaaa tgattttcgt tatgatagct
    13261 acgaaattca aaaaggaaag cccagcttag cgggattgcc agccttatat acggaagatg
    13321 atgatgaagc tgaaacgctg attattcaat tgaaagacgc gattagtgga ttgactttac
    13381 agttgttcta cacaatttat caaaatcaca gtgtaattat tcgttctacg caattagtaa
    13441 ataatagtga tgaaacagtg gagattaacc gcttagctag tcaatcaatt gattttccaa
    13501 atcgtccact cgagttaatt catttaaatg gtacctgggc aagagaacgt caaatgacta
    13561 gagaaaaaat tcatacaggg ataaaagtat tagatagtaa acgcggatca agcagccatc
    13621 aacaaaatcc gtttgttgct ttagtagatg ccaaaacaga tgaatttcaa ggagaggcat
    13681 atggatttaa tttagtttat agcggtaatc acgaaacggt gattcaaaaa gatccatttg
    13741 accaaacacg ggtcattatg ggaattaatt catttaattt tggttggcaa ctaaaaactg
    13801 gcgactcatt tcaaacgcca gaagtcgtga tggtttatag taatgaaggt ctgaatacgt
    13861 tatcacagac gtatcatgaa ttgtataatc accgtttaat tcgtggaaac tatcgtttaa
    13921 aagaacgtcc cacgttaatc aacaactggg aagcaacgta ttttgatttt aacgaagaaa
    13981 aaatcatgaa tattgtcgat gatgcgaaga acttgggcat tgaaatgttt gttttagatg
    14041 atggttggtt tggcggtcgt gaaaatgact tcacttcatt aggtgattgg gttgaaacag
    14101 aaggcaaact tgcttgtggt ttggaaaatt tagccaagca agtgcatgat aacgggatga
    14161 aatttgggat ttggtttgaa ccggaaatga tttctgaaga tagtgaacta tttcgagcac
    14221 atccagattg ggcattgggt attccaggtc gtgaccgctc aattagtcga aatcaatatg
    14281 ttcttgattt ttcaagacaa gatgttcgtg ataatatcta tcaacaaatg acggcgattt
    14341 ttgatcgtgt accgattgat tatgtgaagt gggatatgaa tcgaaatatg acagaagtct
    14401 attctgtctt gttggactca acgatgcaag gtgaagtggc tcatcgttat atgttagggt
    14461 tatatgaatt tttagaaaag ttaacaacga attatccaac tattttattt gaaagttgtt
    14521 cgggtggagg cggtcgtttt gatgcaggtt tcttgtatta catgccacaa acttggacga
    14581 gcgacaatac cgatgccgtg gctcgtctga aaattcaata tggaaccagc ttagtgtatc
    14641 caatttcaag tatgggtgcg catgtttcag cagtcccaaa tcatcaaaca ggaagagaaa
    14701 cgagtttaac tattcgtggc aatgctgcga tggctggtgt ttttggttat gagctagatc
    14761 taacgcaatt aacttctgaa gaaaaagcag aaattaaaga acaagttgcc ttttataaaa
    14821 agcatcgcca acttttgcaa tatggaactt tttatcgctt ggaaagtccg tttgaaggca
    14881 atgatactgc atggatgttt gtctcaaaag accaaacaga agccatggtc ttttatttcc
    14941 gagtattagc agaagctgcg catccattag tcacattgaa attagcagga ttagcagaag
    15001 aagcaactta tcaaatggaa gatgtaacat ttgctggcga tgaattgatg aatctcggtt
    15061 tttatattga ctcagaacta catggtgatt atgcgacaca acgcttttat ttgaaacaga
    15121 tatagtagct tgaatttaca atataagtgc aacaccaaaa gagactcatt ccaaacgaag
    15181 aaaatatgtt ttatttacac gcatttacta aaaagcagcc tgatttgaat tgggaaaatg
    15241 aagaagtacg tgaagaaatc tatgcaatga ttaactattg gttagataaa ggactaggcg
    15301 gatttagaat cgatgcgata ttaaacatca aaaaaaaact agagtatggc atctttgaat
    15361 ctgatggaga agatgggtta gccttcattg ggccctggat tcttaatcag tcgggcattg
    15421 aagtgtggtt aaaagaaatg agagagcgga catttaaacc acataatagt atgacggtag
    15481 cagaagccga tgttcctaat gaacgtttag atgaatatat tggtgaagat ggctattatt
    15541 cgatggtttt tgattttagt tatacggata ttgatgtacc tgaaactggt gaatggttta
    15601 aagatagcca atggacatgg acggatatgc gtaccaatat ttttacgaat caattagtta
    15661 cgcaagataa aggttggggc gccttatatt tagaaaacca cgatcaacca cgttcaatta
    15721 ataaatatat tccagaagaa tggatcaatg attatagtaa aaaaatgttg gcgacgcttt
    15781 ttatgttact aagaggtacc ccttttattt atcaaggaca agaactagga atgaccaaca
    15841 tagaaatggc atcattagaa gattttgatg atgtagctac gcattctcag tataaacggg
    15901 cattagaata tggattggca ccagagcaag cattgaaagc tgtcaataaa cgaagtcggg
    15961 ataattctcg cacacctatg caatggaata cgcaaaaaaa tgccagattc tcaacttctg
    16021 ataatgtatg gttaaaagta aatcctaatt atccagaact caatgcagaa gctcagttaa
    16081 ccaatcaaca gtcggtattc aattattatc atcaattaat taaattaaga aaaaatagtg
    16141 tatacaaaga cgtgctaatc tacggacaat ttctaccagt gaaagataaa aatgaacata
    16201 ttttgcttta tgaacgccaa ttagaagata aaatcgtatt agttatctta aacgttacag
    16261 caacagaaca agaatattat gtagatgaac agtaccaaca agtattaatc aataattatg
    16321 aagatgtttt attagatcaa agtcgaaaat tgcgtttacg accgtttgaa agtattgtat
    16381 tagctaatta tggaggacaa ttatgaaaat taaaaacgaa gcaatgctca tcacctatgc
    16441 agatagttta ggaaaaaaca tgggcgaatt aaaacaagtc ttagacaaac atttacaagg
    16501 tgttattggt ggtgtacatc tgttgccttt ttttccatcg acaggtgatc gaggctttgc
    16561 gccgagtgat tatacgacag ttgatccagc tcttggaaca tgggaagatg tggaagcatt
    16621 aggtgaagat tattatttga tgtttgattt tatgattaat cacatttctc gtgaatcaaa
    16681 atttttccaa gatttcaaaa aaaaccatga gcagtcacca tacaaagaaa tgtttatccg
    16741 tattcatgaa tttttcccag aaaatcgacc aacacaagaa gatattgatt taatctacaa
    16801 gcgtaaagac aaagcgccgt ttcaaacggt agaatttgcg gatggtacga cagaagaggt
    16861 ttggaatacc tttggggaag aacaaattga tttagatgtg accaaagaag ttgtgaaaga
    16921 atttattcga gaaaccatca aagatatggc gaaccatggc tgttctttaa ttcgattaga
    16981 tgcgtttgct tatgcgatta aaaaattaga taccaatgat ttcttcgtgg aaccagatat
    17041 ttgggaactt ttagacgaag tgcgtcaaga agcagcgaaa tatgacgtcg aattattacc
    17101 agaaatccat gagcattatt ccattcaaat gaaaattgcc aatcatgatt attatatcta
    17161 tgacttcgcc ttgccaatgg tcactctgta ttcactttat agtggaaaat cagaacgttt
    17221 agccaattgg ttgaaaatga gtccgatgaa gcagtttaca acattggata cgcatgatgg
    17281 aattggtgtt gtggacgctc gtgatttatt aacagatgaa gaattggatt acacctctga
    17341 ggaattgtat aaagtcgggg cgaatgtgaa gaaggtctat tcaagtgcca attacaataa
    17401 tttagatatt tatcaaatta acagcaccta ttatagtgca ttaggcgata atgaccgtag
    17461 ctatctgtta gcacgtgcga ttcaagtatt tgcgccaggg attccacaaa tttattacgt
    17521 tgggttatta gcaggaaaaa atgatattga actgttggaa gcaacgaaag aagggcgtaa
    17581 tattaatcgt cattactatg atttagccga aattgaagaa gaagtgcaac ggccagtgat
    17641 tcaacaatta tttgcacttt taaaatttag aaatgaatct gcggcgtttg atttggatgg
    17701 aacgattgaa gtagcaacgc catcagaatc agaaatagtg attacacgta aaaataaaaa
    17761 cggtagtgat agtgcaacat taacggctaa cttggatacg aaagaattta tcattgagtc
    17821 taaagatata aaaattttca ctagtgttgc attaaaattt taacctattc catagaacaa
    17881 aaaatccttt atactagcac ttggatgact tatccaagac caataaaaag gactcataca
    17941 tggataagta taaaacaaat acagcattta ataaatggtt ttctgccata aaattaggaa
    18001 aactttctgt caactcccaa aaatgtattt ttgattacaa ttcgtatcgt aaaaaactgt
    18061 cttttgaagc tgtgctcaag ctctttctct acgcagttaa tgaagaaaaa gaaagtttgc
    18121 gtgatttgag tacagctctt atcgaccgat cgctccaact agaaaccgat gtgtcatcga
    18181 ttagtcatac ccaattgtct cgagcattta atgcacttga acctaaagtg ttagaagaaa
    18241 tttttcaaca actgttagaa aaagttcaac atcaaatcaa accaaccaag cgaaatcatc
    18301 tgtatctgat tgattccagt acgttttcgc ttagtttaaa acgtcataag tgggcgactt
    18361 tccgtcaaac aaaatcaggt gtcaagttac acttaaacta ttgttatatg gacgatacta
    18421 cgatgtatcc aactgatttt accttaacaa atgcccatga acatgatctc aatcaactac
    18481 cagtattggt gaatcaacca gaggcaactt acgtgtttga tcgtgggtat taggactttg
    18541 aaaagatgga tcagatgcat tgggatggct atttcttcgt cactcggatt aaaaaaaaca
    18601 ccaaagttca cgtgatagat actctggaaa catcgccaga aacagaaatt ctacgagacg
    18661 agctggttcg tttaggatct aaaacctatc taaccgccaa tttccgacta gtcactgttc
    18721 aagataagaa cggacgtgtg tttcaattta tcaccaatcg aatggacgtc tcttcaaaag
    18781 aaatctctga tatgtatcac gctcgttggc aaatcgaatt gtttttcaag cacatcaaac
    18841 aacacatgac cattaaaacc ttcttttctc aaagtgaaaa aggcgtgcag aatcaattga
    18901 ttttaacgat gatttccgct ctcctcacgt ttctcatcaa attggagacg aaaacggaaa
    18961 aatcagtgtt ccaaatcaaa cgattctttc gttatttact gtttcagccc tttgaatgtt
    19021 gtctagaaaa attaattccc acatgagaaa aaagaaattt ggctcgttac ttttggtttt
    19081 cagttgaata acagataaaa aattttctgg aaaaagtcat cctctgcgtc agcacagttt
    19141 tttttgactt attttcagag gaaatagttt tcagaatatt ctaaaatgtt ttatgcaaca
    19201 ctagtgatat tttttagaat tagatttcta atgtgcattt tttgagtatt aaggagcaag
    19261 tatacaaaga tttataaagc tgattaaatt tgaataaaaa tatgcaggaa cgctactatt
    19321 tttagtagtg ttctttttta tatttgaata aaaaatgtgg tggttaaatg gtggttataa
    19381 caaaaaatga attaatgaaa ttaggttatg ggaagtatca gtctgagaga atcatacgac
    19441 aggcaaaagc aattatggtt tcaaaaagct atacctatta cgaaaataga agattaggac
    19501 tagtacctgt tgaatctgta gaagaagttt taggtatgaa gatagaggta gaggaggatt
    19561 ttcagaatca tggttaattt taaaaaagat aaaaatggga aatatcattt tactgctagt
    19621 ttgggattcg acaaacgaac agggaaaaga gtacaaaaaa acgtagaggg gttaaaacag
    19681 ttaaagaaga aaaaaagcgt ataccgaact tgtttctaat tatggaaaag aagcagaaac
    19741 ttctcaaagt actatgttgt ttgaggagtt tttttataaa ttattcctac cttattatca
    19801 agggaaagta gaagaaagaa catataaaaa tcggttatct acaatcaaaa aaacttttct
    19861 tatttcttta aaatggagtt aagagaaata cgtccaattg acattcaaaa gtggcaaaat
    19921 aatttattga gcaaatataa aaattcttat gtaagaaaca tgtatgggat ttttcagatg
    19981 tcattagatc gagcagttgt tttgggacta cttgaaaaaa atcctgcaaa agttattgga
    20041 aatgtgaaga aaaggaaaac tacagttgat ttttggacaa aagaagaatt tacaaaagtt
    20101 atttcaactt tttatattga tgatttgtac caacattttg gttttatttg tatgtggtta
    20161 ctttttatga ccgttatgcg tttgggagaa gccactgcat taacttggaa agatattgat
    20221 tttgaaaata aaaacttgcg tgtgaataaa acactctatt ataagagagc caaaacctat
    20281 gagtttgtag aacccgaaac ggcttctagt attcgtacaa tcgaactaga cgacaataca
    20341 attacctttt taaaagcttg gaaggagcgt caagtttcaa taggaggcat gtcgtttgta
    20401 ctttcttata atggaacacc tactcagaaa tatacaatcc gtcatattat gagaagacat
    20461 gcaacgatcg ttggtgttca tataatccgc atacacggat taaggcatag tcatgcttct
    20521 ttgcttatat cacaataaaa agatgcactt cgaattaaag aaaggttaaa acatgctgat
    20581 gtacaaacga ctttaggaac ttatggtcat ttgtatctaa attcattgag agagataaca
    20641 aatgatccca cagattatat ccaaactaat ttttctaaaa tcaaaccgac acctatttac
    20701 gataataaaa aataaatgat aaacaaaagt aggatcacac caaaagaaac acactagaaa
    20761 aactataata caggcttttc tagcgtgttg tttcttattt ccgcctattt atacattata
    20821 ctcataaagc atttaatgca tcgatcgtag ccgcattgtc tatatgggta tataaagttg
    20881 ttgtattggt acttgaatgt cccaattgtt gggcagttaa taccaatgaa tttgtctgtt
    20941 ggtagagtct agtagctaat gtatgacgca gtttgtgagg gctgacttga attctaaagc
    21001 cttttgaata ttttgctacc atccgttcga ttgcaatacc ggatatacgc ttggcttctc
    21061 cttggtaaat tgctagaaat aaagcttttt cgttttctgt tactttatat cgttctttcc
    21121 gaatagctag ataattagct aaatattcca ttgcgaatgc tgctatattg acagcatctc
    21181 ttttatttcc ttttctagtt actaccacat tattcttatc taaagatagg tcatctaaat
    21241 taatatttat aacctcggag agtctcaatc cagatcctaa aatcagtgca ttgatcgcca
    21301 ggtctctttc tacatctcgt tgataataga tcaaagctcg tttactcaaa gtatttttat
    21361 attcattggc aatataatct agataatttt gtgtatcctt atctagaaat aattttttct
    21421 caatggcatt ggcacgtgca gctaacgtag ttttatctgt gatggttgct acttgatcca
    21481 taggattatt gtctaagtat ggttttccta gaggattatt ggaacttttc gaaagatatt
    21541 taaatagaac ctttaatgcc gtgatatttc tattgatagt agaagtagat agacctaagt
    21601 tattattatc tttgagctta gtttttgttg tctctttttt tacattttct tttgtgcgac
    21661 gtttcaaatg agatttgtac aagtttaggt cataggcagt taactgtctt atcgaggtga
    21721 tagaagtatc aggaacgata gactcactca gaagccattc ataaaagagc ttataatttt
    21781 ttagatattc taaagtggtt cgaggtgatt gatctttatc ctcacgatct tgaacaaaat
    21841 cttttatata atcgggcata tgaagtatga ttgtatctat tttttgatat atttctgtat
    21901 ttttcatgtc attcatcctt tcaataagta ggggagaaga gtatctctat aattggacta
    21961 taccaattat agaatgtttt gtcaattggt atgctctaaa atttattaat ttgtttcaaa
    22021 aaaagcatat aaacaggtat acgtttaata atttaaaata aatggtatac ataaataggg
    22081 aaggaaatag aagggagcaa tctaaatttt ttgaattttc ttaattaatt atatagaaag
    22141 aacaatcttt ctatagagtt agacatgaga gggactatgc ttgatttaaa atcaacaagc
    22201 gtatatactg tatatacaaa gaaaaaggag ggagaagact atggcaatta aagaaagaaa
    22261 atttagggaa gtggggaact cagtagtatt aactatttca aaggagtttc ttaaagaaaa
    22321 tgggttaaat cccggagata cggttttttt agatgaagaa aaacttcgag cggcaattac
    22381 aaaaaaacaa gccttatctg atgaaattga tttagcaata tctcaagcat taactgaata
    22441 tgatactact tttagagagc ttgtcgataa atgagtattc attacctaga agccaaagat
    22501 attataaaaa tgaatgtaat ccaaataaaa aaatactctc ctaatgaacc ccttggtgta
    22561 aaggatgcga atgctttaga aatgtgtgta gggcaacttc gagcctcagc ttttggtgaa
    22621 gaggtttatc cttcgattta tgaaaaagca gctatcctac ttatacaatt gataaagaaa
    22681 catccttttc acaatgcaaa taaacgtact gctttcttag ctacttttgt ctttttgaaa
    22741 atcaatggac agcttttagc tattgatcaa aaagatgctg taaatttagc ggtatatatt
    22801 gctacgtatg ataaggattt cgatcaacta aaatatgaag tgatcaacac tataaagaca
    22861 catacgcagc ccatttaagg agttactgta tgtgtcttta tagggttatt agctgctata
    22921 gcgaaataat tgaagtaaat tacaaatagc tataattcaa atccaactat atagaaaacg
    22981 taaattttct atatagttgg attaagataa cgactattta ttttatcata tttttactat
    23041 accctatact tttacttggt atatttatga ataatcgcaa atttacccct atactactag
    23101 tttgtaagtt agattaataa aaatactaaa gttatttgtt cttttattaa atacacctac
    23161 aacttataaa ttgatggtat ttagataaca cgaaagaata aaaataacag ctgatagtaa
    23221 agtttgttga cgattgctac tcaatcgaaa gtgagttagt gcgtatgaac ttgcttctga
    23281 aaacttatga aatgaaagac cttttacatc ttgcagaaat cggtactggg attaaactaa
    23341 gcaataaaaa ctaatgttcc cctcattctt tgaacttcta ctatttgatt tatttattct
    23401 gataaatgac gatttcacct tcaggatctt caaatcccgt atagaattta tttaagatct
    23461 cttcagtatc agtaatagga taggcgatag gccacatacc ctttgatggt tagaataaac
    23521 tgctcaaaat ctgtatcgta tgttggcttt agcatattaa caaaggctat ctccaaatta
    23581 taaaccataa gcgttgaatc gttcttaaca atttcaattc ttcctaaccc aaagtcagaa
    23641 gatataatta ccggacttgc cttacccttc atgtgggttg ttgagattcc atatttaaag
    23701 ggaattgtcg gtactatcgg cggtctgaaa aacatcccaa aaataaaaaa atctttcccc
    23761 tgctagtctc ctaagtccct ttgtagcgga tatggcacca taatctcatt ttatcgaagt
    23821 gagtcatggt ttatctgttg gccaaccaaa attgatacaa tctaacgatg caccctctac
    23881 ggcattctag gggagtgcac ttgcattcac ccaataaaaa ccggcagtta gcaaagaact
    23941 tatgtagtcg tatgttctta ctgttaacat acaagaggtc tcctttccac aattgcttag
    24001 cagtaaacgg gaatttacaa agctaatctc ttccattttc ttatctttgt tctaaaagtc
    24061 ccaaatggag caactgtatt tacatgaata aatttatata cttcccatac agccgttttg
    24121 gttgcctcgt ctgcccattt tcttttgtgt gcagtgaata gatcttcatc actcattgaa
    24181 tcaatcatca tgttaatatc atcaatatta cgattgagaa tttcctctaa ttctttcaat
    24241 ggtagatgtg cataggtatt tgtaaaccac ttatataact ctcctaactg attccattta
    24301 aattgatcag aaggcgtttt aacagataat ccatttcttt catcattttc ccattttaga
    24361 attaaattcg tccaaccaac ttgatatgct aaattttcag caggtgttct atccacttcc
    24421 tcaactcttt tatccttcaa ttcttcagga atagtttcaa attctaaaat atatttagta
    24481 taagtcctat ttatttcttt ttttaattct tctttatttt tatatgttct cattatatta
    24541 cctccataaa ttcctaattg tctcctttaa ttcatctata atttcatcca cgatatttta
    24601 atccgttcta caatgcatat ctgatatgca ttgactgtct aactcatatg tgaatgattt
    24661 ataaaatatc ccaacgtctc atctaaagaa atgttgatcg tcagcaagga gctaaaaaat
    24721 acgaatatat ttcttttgta gtaaaatcgg actggtttgc atatcatttt cttcgtaaat
    24781 atcaaaatca tgttaactac tatcaattac taataaaaac taccacttta agcgttaaga
    24841 actgttaagt ttagttaagt gaggatagtt cacaaaatcc attgttaaac ttcacgaaag
    24901 tggtaaaact caaaatgctt tatataggat tacagcatcg ctctttcttc gattgcgcgt
    24961 tgggtaaaaa atattatgaa gtaaaaattg acgatgacac gttccttacg gctcatcaga
    25021 tgaaacagct acaaaaacga aatgatcaac tcgaagaaaa tttaattcta aaaaagcgat
    25081 tgccatattc acgcctcgct cgacgaacta ttaaaggtca agatttacct gaagatcatc
    25141 ctttttagga aattgaagct ttttatgttg cacaatgcat ttataatact acaatgatgt
    25201 tttcaccaga tattcttcta gggtgcggtg taatgaaaca gatacacttg caaaagaaaa
    25261 ttcaccgcga atttactaaa attatgaata attatgttaa tcttccgaaa tttttttcca
    25321 aaattaaggg atcaagctgg tgttatagat tgtttaaggt tggcaaaaaa ataacttctt
    25381 tttaaacaat tatagaaaac cgatactctg ttaagttggt tgacacggtg tcgattttta
    25441 tgactttctt attctgaaac ttcactaacg gacagtacct aaaaagtcta tagtatggac
    25501 gtttacgagt actattttat caaaaatgct gctcatttca acttatatgt tttagtgaac
    25561 tacccccact tagcttggat aggctacaat taactagaca gaaaaattaa ggtgtgtaga
    25621 ctagaagaaa acataccagg aggaattttt atgtctaaga gaacacgaag aactttttca
    25681 caagaattca agcaacaaat cgtcaatctt tacttagctg gaaagccacg tgtagaaatc
    25741 attcgagaat atgaactaac
//
LOCUS       DAJSAW010000035        25689 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJSAW010000035 DAJSAW010000000
VERSION     DAJSAW010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.035"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            108..530
                     /locus_tag="ONT64_001685"
     CDS             108..530
                     /locus_tag="ONT64_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2303103.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(696..1889)
                     /locus_tag="ONT64_001686"
     CDS             complement(696..1889)
                     /locus_tag="ONT64_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2303104.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            2113..2490
                     /locus_tag="ONT64_001687"
     CDS             2113..2490
                     /locus_tag="ONT64_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR2303105.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2478..2816
                     /locus_tag="ONT64_001688"
                     /pseudo
     CDS             2478..2816
                     /locus_tag="ONT64_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2950..3393
                     /locus_tag="ONT64_001689"
     CDS             2950..3393
                     /locus_tag="ONT64_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HCR2303106.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            3654..4469
                     /locus_tag="ONT64_001690"
     CDS             3654..4469
                     /locus_tag="ONT64_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2303107.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            4479..5141
                     /locus_tag="ONT64_001691"
     CDS             4479..5141
                     /locus_tag="ONT64_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR2303108.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            5349..7013
                     /gene="alsS"
                     /locus_tag="ONT64_001692"
     CDS             5349..7013
                     /gene="alsS"
                     /locus_tag="ONT64_001692"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCR2303109.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            7026..7736
                     /gene="budA"
                     /locus_tag="ONT64_001693"
     CDS             7026..7736
                     /gene="budA"
                     /locus_tag="ONT64_001693"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCR2303110.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            7866..8363
                     /locus_tag="ONT64_001694"
     CDS             7866..8363
                     /locus_tag="ONT64_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCR2303111.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            8548..8808
                     /locus_tag="ONT64_001695"
     CDS             8548..8808
                     /locus_tag="ONT64_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303112.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(9170..9409)
                     /locus_tag="ONT64_001696"
     CDS             complement(9170..9409)
                     /locus_tag="ONT64_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303113.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            9695..10009
                     /locus_tag="ONT64_001697"
     CDS             9695..10009
                     /locus_tag="ONT64_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303114.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(10443..10809)
                     /gene="ssrA"
                     /locus_tag="ONT64_001698"
     tmRNA           complement(10443..10809)
                     /gene="ssrA"
                     /locus_tag="ONT64_001698"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(10980..11753)
                     /locus_tag="ONT64_001699"
     CDS             complement(10980..11753)
                     /locus_tag="ONT64_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2303115.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            11897..12247
                     /locus_tag="ONT64_001700"
     CDS             11897..12247
                     /locus_tag="ONT64_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HCR2303116.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            12228..12944
                     /locus_tag="ONT64_001701"
     CDS             12228..12944
                     /locus_tag="ONT64_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2303117.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            12944..14392
                     /locus_tag="ONT64_001702"
     CDS             12944..14392
                     /locus_tag="ONT64_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2303118.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(14462..14971)
                     /locus_tag="ONT64_001703"
     CDS             complement(14462..14971)
                     /locus_tag="ONT64_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303119.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(14961..15686)
                     /locus_tag="ONT64_001704"
     CDS             complement(14961..15686)
                     /locus_tag="ONT64_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303120.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(15909..18029)
                     /locus_tag="ONT64_001705"
     CDS             complement(15909..18029)
                     /locus_tag="ONT64_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR2303121.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            18259..19437
                     /locus_tag="ONT64_001706"
     CDS             18259..19437
                     /locus_tag="ONT64_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCR2303122.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            19453..20211
                     /gene="aroD"
                     /locus_tag="ONT64_001707"
     CDS             19453..20211
                     /gene="aroD"
                     /locus_tag="ONT64_001707"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR2303123.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            20234..20719
                     /gene="ybaK"
                     /locus_tag="ONT64_001708"
     CDS             20234..20719
                     /gene="ybaK"
                     /locus_tag="ONT64_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCR2303124.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(20790..22043)
                     /locus_tag="ONT64_001709"
     CDS             complement(20790..22043)
                     /locus_tag="ONT64_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR2303125.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            22161..23510
                     /gene="gdhA"
                     /locus_tag="ONT64_001710"
     CDS             22161..23510
                     /gene="gdhA"
                     /locus_tag="ONT64_001710"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR2303126.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            23622..24968
                     /locus_tag="ONT64_001711"
     CDS             23622..24968
                     /locus_tag="ONT64_001711"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR2303127.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(25276..25662)
                     /locus_tag="ONT64_001712"
     CDS             complement(25276..25662)
                     /locus_tag="ONT64_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR2303128.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         8498 a         4425 c         5131 g         7635 t
ORIGIN      
        1 gagaaataat aacaggttta tgcactattg acggtgtgac aatatgatat ttgtatccgt
       61 ttttttcttg tatgtagtat agtaaagtag atacaggagg agaaaatatg cagacttttt
      121 taacaaaagt ttctcaaaag aaaattaaaa tgattcattt actgcttgaa acagaacgtt
      181 ggtgttcaat agaagaacta caaaacgaat tgaaggtatc aagtaagtct atcttgaatt
      241 atctaacaga tttagaagaa ttgttccaac aatatcctga taaggtcatt ttgaagaatg
      301 agaataatcg gcgcttttct atggaaaagc aggagaactt cccaatctac attatttatc
      361 ttcatttcta cagaaaatca tataattatc atttgattga attcatgtat caacatcctg
      421 agaaaaattt ggaagattat gcagacgctc aatatacgag tgtatccaca gtttttcgtt
      481 atgctaagtt attagtaaag tactttgaaa tgacggacgt caaagaatag tcgccgccag
      541 atagaaggaa attagcagtt gaaaaagctg ccctgcatca agtaatacga ccagccaaac
      601 ggaaacagtg tctcatgttt ttgatttggc tggtcttatt accgcagatg caagcttttt
      661 ccacacctct ttcttgtcat ttacagactc aactatcaat gctgcaagct ttcccttaat
      721 gctgtaacac cagtaagagt tgtcaagacc agcagactga ctaggtaaat accgctcaaa
      781 aacaagatta cggcagttaa actataatga tccatcaaga aaccgatgac gatagaagaa
      841 aaaccaccaa cagctcgtcc aaggttcata acgaaattat tcgccgtcgc gcggatttct
      901 gttggataca aactactaat cactgcgcca tatccaccat acatgccatt gataaagtag
      961 ccgactatca cagcggctgc taccaaactc cactggctat gggctaacac aagtagatag
     1021 acagataagg atgaacaaat taaaaacaaa gaaaaggcta gtttaggccc caatttatcc
     1081 atgattatgc caaaagtcat catccccaaa gacatgccta aaatagtact gaccatccac
     1141 aatgaagacc cagaaaccga aagccctaat cgattttgca taattgaagg tagccaattc
     1201 attaacccaa aatagccggc aatttggaca gttaccatga tactaaggcc acatgtttgc
     1261 caagcaagat gttttgattc aaacagtctg ctaagtttgg ttttcttttc ggcagattgt
     1321 acagatttga attcctttgg ttcagtcaag ccgcgacgaa tgaacaagac catcaacacc
     1381 ggcagtacac ctacgacata caacatcttc caaccaaacg ttggaatgat cacagcagct
     1441 aaaatcgcag ccaaagccgc accgacctgc gcaccaatac cagcaaccga agaagctcgt
     1501 ccaatttgtt tttttgaaaa actttcggaa atcaaggaca tacacgcccc atattctcct
     1561 cccgctccta cacctgcgat aaagcgaaag agataaacca agtagatgtt tgaagcaaaa
     1621 tacatcaata aagaagcaat cgaaaacaga aggaccgttt gagaaaatac tttcacacgt
     1681 ccatatttat cagctatgat gccaaaaaaa atacctccaa tgagcatccc caaattagtg
     1741 atcgttgaaa tgaaacccgc ttgcgtacca ctaatatgaa aagaagtaat cattgaagat
     1801 aatgaaaaag ctaaaaacat aatatccata ttctctaaag caattcccgc cgatgtcgat
     1861 actaatacct ttcgctgata cgttgtcatt tttatacccc ttttcctaaa atttccctaa
     1921 attgttaaaa gtatatccaa aaaagaataa aaaaacaaga cttttttatt tgaaataata
     1981 cacaaacaac cgatatagat aatccagctt agttttaata gaaatgagta ttgacttgga
     2041 gtacacttaa agttgtagaa taaataaaat acataagtaa agtggcaaga aattaagaga
     2101 aagagtgatc agatgaatat caaacaagtc agtgaagaaa agggcatttc ggccgataca
     2161 ttgcgctatt atgaacgaat cgggttgatt ccaccagtta atcggacaaa aggcggaatc
     2221 cgtgattata cagaagaaga cctaaaatgg gtcgacttta cgatctgtat gcgaaatgcc
     2281 ggattatcga tcgagtcttt gattgaatat attcgcctat ctagcgaagg agaagcaact
     2341 gtatttgagc ggcgtaagct tttgattgaa gaaagtgaac ttttgacgaa aaaaattgct
     2401 gaaatgcaag aatgtttgga acgtttacaa ggaaaaatca agaaatatga tcgtgtgtta
     2461 tctagaaata aaatggaatg tatcaagtaa aaaaatcaag gagcatcgct atggagttac
     2521 gcttactaaa atatttttgg acaattgcag aagaaggaac aatctctcaa gccgcagaag
     2581 ttctacatct tacacaacca accttaagcc gacaactgaa ggagttagaa gagtaactag
     2641 gaacagaact gtttgtacgc gaaaaccgca aaatgatctt gacagaagca ggttattttc
     2701 taaaaaagcc gagcagaaga aattttagat ttaacgaatc aaaccactca agaattcgag
     2761 aacagaaaaa aacaattatt cagcggccga tttacgatcg gctgtgtgga agctgatagt
     2821 tcgaatacat tggcactatt actagaaaag ttgatccgtg attatcccag cgttcaattt
     2881 catatccaca gcggaacaag tgatgatatc acggagtgac tagaaaaaag cctgttagat
     2941 ttagcgattt tgctagatcc agtagataca gagaaatacg aaacgatttt gttaccaaga
     3001 aaggaaaaat ggggattgct cattttagaa aattctgttt tagcaaaaaa agacgtgata
     3061 acaaaagaag actttgcgaa tgtacctctt ttgatctcaa agcgtccaga aatacagcga
     3121 atgtttgctg actggttagg cataaaaaag gagcagttgc tgatcacagg aacgtacaat
     3181 ttgattttca atgtatttgc gctaattgag aatcaggtag gtgctgcttt agtcatagaa
     3241 ggtgctgtaa gtcatcattc aaagcgagtg acattcgttc cgcttgaccc aatgattgaa
     3301 acgaactgca tgctagtctg gaaaaagaat cggattttat cacctgtagt caatgaattg
     3361 attcaccgtt tcaaagaagc ttttgaacag taataagtat ctctaatcga agaaatttca
     3421 atacaaaagc gagacgtatg acagtcttgc ttttgtattt tttcattttt aaatcaaaaa
     3481 aataaagaga tttacataac ttgttaaata agcgcttaca aaaataagaa gaacggaaat
     3541 gaatcagcta taataaccgt tgttctggat tgtaatcatt acaaagaaat gaagattata
     3601 actttggagg tgttcgaaat gaaagaaaaa aagcaaaaat tcgttcaaaa aacatgccct
     3661 ttattcaatg gttgggtatg attctgctga ttgtattagc tttattgacg atcattcctt
     3721 ttttatggat ggtttctgct tcattcaaag caaataatga agtgttcacg attccgattc
     3781 aatggatccc taaaacttgg catttagaga attatctggt tatttggtct cgtattcctt
     3841 tagtgacatt ttttaagaat acagcttttt taagcgttgt gatcacgttg attcagttgt
     3901 ttacttctag ttttgctgct tacgggtttt ctaaactaaa ttttaaaggg cgcgatattt
     3961 tatttttagc atatatcggc acgattgctg tcccttggca gtcttatatg atcccgcagt
     4021 tcattatgat gagaaaagtg ggtttaacag atacgttgtg gtcgttggtg ctattacaag
     4081 cgttcaatgc ttttggtgtg ttcttgttga agcaatatta cagtagtatc ccagattctt
     4141 tatgtgagag tgcgcggatc gatggcttat cagagtgggg aatctatcgc aagatcattt
     4201 tacctcttac aaaaccggcg attgccagtt tgacgatcat cacattcgtt aatacatgga
     4261 acgattatat ggggccattc atttatttgt cttctacgga aaataaaaca atccagttag
     4321 ggttgaaaat gtttattggg ctatttgatg cagagtacgc attaatcatg gctgcctcag
     4381 ttgtttctgt tcttcctgtt gcttttatat ttttagcgat gcaaaaatat tttgtagaag
     4441 ggatcgcttc atcaggaatg aaggaatagg aggtgattat gaaagagcag cgttttgaca
     4501 aaaatgttta tatggaattc ttttattggt tgtatcaatc attggcaagt agcctttgtt
     4561 tttggtgtgt caatatcttg tatatagcag ctgcgttcct tttgtcgata gatggaagga
     4621 atgtcttttc ctttattttt tctttacttt ttattggtcc aggttgcatc gtgatgatag
     4681 gtatgacaga agaattagca aaagaaaata gtttttcacc agtacgtttg ttcataaaga
     4741 tgtttcgcca attttggcta aagggatttt tttattggct atttgcttgg atagttagtg
     4801 tgataatgat ttttgattgt ttctttttta tacgtttttc ttatggaaaa tggttgattc
     4861 cgttgtttgt tttacttgct tgtttatcag tttcctttag caccaactgc tggtattttc
     4921 aggtgagaaa tccagcgtcg aaaccaaatc aagttctacg gatagcgttc tactatacgt
     4981 tgaaaaaatg gtatgtgagt ttactagatt ttttactatt gacaagtttg tttctcttct
     5041 ttttcgtaaa gccccaatgg tgtattctac taggtccttc tatcgtattt ggtttgattt
     5101 acttcaacaa tcgtaaattg atgagaacta tggatctgta gactttataa attgaaactt
     5161 tcaattaaga atgacagttt tttttatgta aaaactgtca ttctttttca ttttttgaaa
     5221 catatggcta aacaaatgaa ataaaacgtg ctatactttt gtatgaaaca ggtttagaaa
     5281 ctgtatcact tatttgtgat tatttctaac taataattaa aaataaagca taaaaaggac
     5341 gatgttcaat ggctgaaaaa atgaaacaag gttcgaaatc tgtagtagaa agtttgatta
     5401 atcatcatgt tgattatgta ttcggtatac caggtgcaaa aatcgatggt gtatttaatg
     5461 aattagagga tcaaggtcct gaattaatcg tgactcgtca cgaacaaaat gctgcattta
     5521 tggcacaagc aatcggacgt attacaggag agccaggagt agttatcgca acaagtggtc
     5581 ctggtgctag taacttagct acaggtttgg taacagcgac agcagaaggt gacccagttt
     5641 tagcaattgc cggacaagta aaacgaagcg atctattgaa gttgacccat caaagcatgg
     5701 ataatgcagc actgttccag ccaatcacaa aatacagtgc agaaatccaa gatccagaaa
     5761 caatttcaga agtgattgcc aatgcttatc gtatggctaa gagttcaaaa aaaggcgcga
     5821 gctttatcag tatcccgcaa gatgtagtgg atgcacctgt ccaaggtaat gtcatcaagc
     5881 cattgagcga tcctaaatta ggttctgctt ctgcagatga tatccgttac ctagctgaac
     5941 gcattagaga agcaaaacta ccggtactat tagtaggtat gcgaggatca agtgaaaaag
     6001 aaacgttagc catcaggcaa ttagttgaaa agacagcatt gcctgttgta gagacattcc
     6061 aagcagctgg agtgatctca cgtgaattag aagcgcattt ctttggtcgt gtgggtttat
     6121 ttagaaacca accaggagat atgcttttga aacgaagtga tctagttatt gcaattggct
     6181 atgatccaat cgaatatgaa gcaagaaact ggaatgcgga aaaagatgcg cggatcattg
     6241 tgatcgatga agcgccagct gaaatcgatc cttttatgca gcctgaaaga gaattgatcg
     6301 gtgatatctc agccactctt gatttattga caggttcatt agagcctcaa caagtgtcag
     6361 aagatgcaaa agaatattta gcatctcttc aagcaaaatt aacggagcgg gacattgttc
     6421 aatcaaaagg agaagcaggt atcttgcatc ctttagaagt tattaacacg cttcagtcaa
     6481 aagtaacaga tgatatgact gttaccgtag atgtaggtag tcattatatt tggatggcac
     6541 gccactttag aagttatgaa cctcgtcatt tgttattcag taatggtatg caaacgcttg
     6601 gtgttgcatt gccatgggcg atttctgctg cattggtgcg tccaaataca caaatcgtat
     6661 cagtttccgg cgatggggga tttttattct ctgcccagga tttagaaacg gcggtacgta
     6721 aaaaattgaa tatcgttcat ttgatttgga atgatggaca ctacaatatg gtagagttcc
     6781 aagaaaaaat gaaatatcag cgggcgtcag gtgtagactt tggtccagtt gattttgtga
     6841 aatatgcaga agcttttggc gctaaaggaa tccgagctac aagtgtagag gaattagaaa
     6901 aagctttaga agaaggattt gcgacagaag gacccgtaat tattgatatt ccgattgatt
     6961 accgtgataa tgagaaattg ggagaaacaa tcttaccaga tcaattctat taaggaagtg
     7021 tcgacatgac tgaaaaaatc ttataccagc acgggacatt aggagctttg atggccggct
     7081 tgatggatgg aacagaaacg attgctcata tattagaaaa aggaactctg ggattgggaa
     7141 cattgcatgg tcttgatggt gaagtcatct ttttagatgg cgtagcttat caaggaagat
     7201 cagatggctc agttgcccaa ttagatggaa gtgaacttac cccctatgca gcaattacag
     7261 attttacgcc agatactagt ttttctgtct caaaaactgc agatggagaa caattgaaaa
     7321 aagacatatt agtaagagaa gccggggaaa acctattttt ggcagtgaag ataactggat
     7381 tattcaaaaa tatgcacatc cgtatcatgc cgaagcaaga aaaaccttac agaagactag
     7441 caaaaatttc agagtctcaa ccagaattcc aacaaagcaa tatccaagga acattagtcg
     7501 ggttctttac gcctgaatta ttccaaggag ttgctgcagc gggatttcac ctgcatttta
     7561 tcgatgatac acatacattt ggcggacatg taatggattt tgaagtggct gaaggaaaag
     7621 ttgagattag ccgcatctcg tcccttgtac agcattttcc agttgatgat ccgacttttg
     7681 ttcacgaaac gattgattat gctgatttag cagcagaaat ccaacaagca gaatagaaaa
     7741 agacagcgag atgcgttcaa ggtgattaac ctgacgtatc tcgttttttt gaggaggaaa
     7801 cctagcgttt acaaaaaaac tgagctataa tcttctccat agatcaaaaa ggagcggttt
     7861 ttatcatgtg gacagaaaaa cagaaaaaag atgcatacga aattttatta ttacagcaac
     7921 aaggattatt agaagcagaa gataactggt tagcaaattt agcaaattca tctgcattgt
     7981 tgaatgagac tttgcctgag actgtctttg caggatatta tttattcgat ggtaatgaat
     8041 tgattttagg accgttccaa gggcgagttt cttgcacacg tatcaagatg ggaaaaggag
     8101 tttgtggaga atcagctgaa aaacaagaaa cattgatcgt tgatgatgtc aaaacccatg
     8161 caaattacat ttcctgcgat tcagccgcac aatcagaaat cgtagtgcca atggtaaaag
     8221 aaggtaattt aatcggtgtt ttagatatcg attgcggagt aacgaaaggg tatgatgaaa
     8281 tcgatcaagc atttctagaa aaatatgtag atcttttatt agcaaatttt aatataaatg
     8341 ctttttcatt tattaataac taaaatcgtc tattgaaaca aaaaagaaga gaaatcaaga
     8401 ataaaaagag ccggaaagac agtggtcatt tctgtttttc ggttttttta tttttttata
     8461 aaatcacaag tgttattgat tttcacaatc atattttaat ggtattatca ttttgaataa
     8521 tgaaaaaaca ttaaggatgg atgaaatatg aataacgaaa ctatcatttt aggaaaaaaa
     8581 tatatttgcc aacctattgg cttgaaacaa aaagtggttg gagaagtaat cagtaaatta
     8641 gaaaattgtg ttgtgatctg cgtagacgaa tatcatgacg tcgatcgcaa tgaaatatta
     8701 gaaaaatgcg gaaaagtagt ggcaaaatat actcacgttt atgagttgga aacaacagaa
     8761 tatctatata actatagcaa caaatatgaa catactttag tttcataaag atttaaataa
     8821 agtgccttca aaagcgagaa gaccaagctt ttgaaggcac tttatttagt tggcagtgat
     8881 tgttcatgat ttttgattta ttcaaaaggt tcgcctagag aatctgtctc tgtaacgagg
     8941 cctggtggag aaactcgata aaaacctata ctgccagaac tgtcctattt tcggatagac
     9001 aaactagtag ttttaaaaag atcatccgat ccaatcattt caaaaaatgc aaaactgata
     9061 tcaggatcat ttagctcagg cttgttatcg taaagcattt gtaaaggttg catttagtta
     9121 ggagtaaacg cgaaactatc atcattattt gctgaagaag atgaagaagc taattcagag
     9181 tttgtttgaa ataaaggaga cgtgctgaca gtttctttac cctgtttttt gcagaggtag
     9241 aaacttttat atcggaagtt tctaccggag aatttttctc atttgtagaa attgttgaat
     9301 cgatactatt cgaaatttgc gtatctgaag gtatgttctt tgatgcagaa gtcccacaag
     9361 cagtaagtaa caataaattt gataaactga taaacagaat tcttttcatt atgtacacac
     9421 tccttatagt atcgtttagc atacacctat gctcagtatt aggcaaaatc aatgcaaaac
     9481 tatcaagtct tttatagtat ctattagtct tagtaataca tatatggaga gggtgatatc
     9541 ccagttcaaa ccagatacat tggttaccat ctgtattaac gattatgcct ctaaacgctt
     9601 tttttatgaa aaaatttata agtgaaaacg taggtatgat attatctaga ggtaattttt
     9661 ttgggtattt aaatagttgc taggaggata gcttttggat ttttacagta attttatctt
     9721 aataatagca atattattac ttctaaatat ttggtttttt gataagagta gaaatgcagg
     9781 gataggtttt agaaccaaac gaagtacgtc ttctgaaaaa aagtgggtat attcacagac
     9841 tattttttac ggaggggtta tttcaatcag tttactttct tcaacacttt attcttttaa
     9901 tgttatagac gtttccatgt caaattttat atctattatt ggaatcctta tttctgcaat
     9961 tattacacaa ctacttcttg tgtttgaaga gaagtcaaaa aataattaaa gcaattatgc
    10021 cttttagtac aggaattatt acttcttaca aatgtatagc gaatgtgaag cctgctgttt
    10081 taattgttct agaaaaactt ttgagtgaac aattatttta tgttaggatg aattaatttc
    10141 tctagagttt ataattaaaa aacttatttg aaggaacatg cttaaaccat aattgtgttt
    10201 ttccttccat cagcataaaa gttcacctca aaaagagtgt acgaacaaaa gttctttttg
    10261 taaaccttaa aatagatttt cctgaatacg tattggggat ttctaaatgt ttgaatgttt
    10321 ttttgaatgc aaaatcaatg caaaaaattt tgtatttctc tacttttctc tccttgacca
    10381 aaataaaaaa acgttgattt aacaacgttt ttcatacatg tttatctgtc tcaaccatac
    10441 atatatggag acggcgggag tcgaacccgc gtccaaacac atcgccactt aaaaatctac
    10501 gttcatagtc aactcattta aggattcact ttacagcttg ccgagtgaca ggcattctgt
    10561 atcgctagtc tgatgttctc tttttcaact tacagacgga aagttgaaac gtatcccact
    10621 aaagttagga cccttactcg agcacatggg cgatgccgag aggatctacg ctaactgttt
    10681 ttaggcagct aaagcgtaag agttgttttc gtttttagca gttatattta actgtaacgt
    10741 ttttacgtag acgtaaccta cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt
    10801 aacgtccccg ttagctaaca agggttacgt gtatttttca tacaaaatta gtatagcata
    10861 tattgggata aattcaataa aaaagcgaat catatcccat tatttatata ataactgccc
    10921 ctttcttaag aaaaaataag cgattttctt aagaaaagag gtagcttcaa aaaaagatct
    10981 taaatcagat caaaatactt caatgcgtgg acaattccgc cttctgtatt tttctttgtg
    11041 ataaaggtag aaagttcttt taatccgtca taggcgttgc ccatagcgat tttgttgtcg
    11101 caagcttcca acaaggcaaa atcattcgga ccatcgccaa atccaaaagt aggaactttt
    11161 tccagctgaa gttctttttg cagacgtttg acacctgccc cttttgagat tcctttcttc
    11221 actgtatcga tcgagtaagg accattccga taaaaagtta gctcaggaaa atgttcgtga
    11281 tagtattcgt cacctgattg agataagatc agcaacatat ttactgtgtt ttctttaaag
    11341 cctaaaggat caatttccgg aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt
    11401 tggctatgac cggtacacca gattttctga tcgttatagt aagaaagttc atgaccgttc
    11461 aactgtacat gatcatacat tttttgacat tcttctgaag aaaatgtttg agaatagatt
    11521 tcttcgccgt ctactcgaat gaaagaacca ttcatggtga ccgcagaagt aatgcccgca
    11581 tcttccatga tagagtggat ttctgcttct gtacgtcctg tagcgatgac ggggagaaca
    11641 ttgttttctt ttaatgcgtt catagctttt gccacttcgg gtgtgatttt tgatttcgca
    11701 tctagtaatg ttccatcaag atcaaagaaa gtgattgcac gataatttga cacaaataaa
    11761 actccttttt tattggaata agtttatctt aacaaagaat gaacaaaaga caaacctttc
    11821 gtgaagaatt cctgaacata ttggttcagt caacaattta gagtatacta taagaagaga
    11881 atttaggagg gaaagaatga tttttttcag tattggtatc ttattcttaa taattggcag
    11941 tctttttcgg attctgccct caaaaggaaa tctgcctttt tatgggtatc attcgcctct
    12001 tgccgcaaaa acagacgctc attggcggct ggctcaaaag acaagcggaa actggttttt
    12061 tcttatgggc ttactgatgg cattgatcgg ctattattta aagacatctg ggcatacgaa
    12121 ttattttctg attgaaatgc ttctgctagt atttccaatt atgcctattt ttattatgac
    12181 agaaaagaaa ttacagaaat atgacctgga aactggaggg aacgataatg aatattttaa
    12241 tgattgaaga taatcaatct gtttctgaaa tgatgcagat gtttttctta aatgaaggtt
    12301 gggaagcaac tttcaagtat gatggaaaag aagggctcga tgccttttta gaaaacccac
    12361 aacattggga tatgattaca ctagacctga atttgcccac actggacggt atgctagtag
    12421 gaagagagat ccgcaaagtt tcaagtactg tgccgattat catgctgaca gctagagatt
    12481 ctgaaagcga tcaggtgatc ggcttagaga tgggggcaga tgattatgtc acaaaacctt
    12541 tcagtcctct gacgcttatt gctcgaatga aagctttgca tcgccgtagt gagttggcag
    12601 aagcaagaga agaagcagca gtttcagatg atcattttga tatcgagact agtcatttca
    12661 agatgaatac gaaaacacga gaagcgtatc tggacaaccg gccaattgaa ggactgacgc
    12721 ctaaagagtt cgatctattg tttactttag caaaaaaacc acgtcaagta ttctctcggg
    12781 agcagttgct tgaattagtc tgggattatc agtactttgg tgatgaacgg acagtggacg
    12841 cccatatcaa gaaattacga caaaaaatcg aaaaggtcgg accacaagtc atccaaactg
    12901 tttggggagt cggctacaaa ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca
    12961 gcaactctta gctttttgcg gaatggtcgc gttgatctta ttgatcgttg gagtttcttt
    13021 tacacaactg acaaaacaaa cgattgaaga aaacaattac cagcaactgt ttggatatgc
    13081 tgaatcagtt gaaaagacaa cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga
    13141 tcaggcgttt caaaatgcat tgctgctgac agagcaagta ctgacagaac aaaatgtgaa
    13201 ctttatattt atcgataaaa acgaacgtgt catttatccg actaatacag ctttactgaa
    13261 ttttacctta acagctgatc aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac
    13321 atcgaataaa aatattttag gacaagcaca ggcgacctct tacgcacttg tgccatttaa
    13381 tttgaatcat gaattctatg gagcacttgt agttagtcaa cctgcgcgaa atatcgataa
    13441 cagcgttcgt ccaattacac tgaacctgtt caaaggattt attttttcca gtatcgttgc
    13501 agttattgcc agctatgctt ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa
    13561 tgcgacgaaa gaagtgacga atggtaattt tgatgtacag ctccccgtac atgacaaaga
    13621 tgaatttgat gagttagcag atgatttcaa taaaatgacg aattcattga aagaatctca
    13681 agcagaaatt gaagaacaag aaaatcgtcg aagacaattt atggcagatg cttctcacga
    13741 gatgagaaca cctcttacga caatcaacgg acttctggaa gggttagaat acaatgcaat
    13801 ccctgaaaat cagcgagaaa acgcgattaa attgatgaaa aatgaaacag agcgtctgat
    13861 ccgtttggtg aatgaaaact tggattatga aaaaatccgt acgaatcaaa tcagtatggt
    13921 cattaaaaaa ttcaatggaa cagaaacatt gaaaaatatt gttgcacagt tagaagctaa
    13981 agcagaagca gcaggagata cattgacatt gaaagccgtt gatgatattg atgtttacgc
    14041 tgattatgac cgatttgtcc aaatcatggt caatattatt caaaacgcga tacagtttac
    14101 tgaaaacggc caaatcacga ttactttaga aaaaggctat cttgaaacga tcatcacgat
    14161 tgaagatacg ggaattggaa tgtctgaaca acaaatgaaa agtatctggg atcgttatta
    14221 taaagtagat ccttcaagaa aaaatacaaa atatggtgaa tcaggattag gtttaccaat
    14281 cgtgcagcaa cttgtacgct tgcataaagg aaaattagaa gtagaaagcg aactaggaaa
    14341 aggaacaaaa ttcgctgttc gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag
    14401 cggtataaaa gatcatgaat cgatcttcta taccgcttgt ttttactata aaatgattga
    14461 tttagcccgc gggctctaaa cgatcaatat aaggactttg agcaatagta gcatcatgag
    14521 taaagagtaa gataccttga ttttcttcat gagctaaata agaaagcaag tggagtagct
    14581 cttggatctc caatacacta agttcatcaa atgtatcttc aataacaata ttgtttcgct
    14641 tggaaagcaa ggcatgaagt aattgcagct ttactttttc aaaagacgtc aactcagctg
    14701 ccgcacaatt aagcaaggaa ggagagatat caagtttttc ttgataggcc aaacgatctt
    14761 gtttgatttc tttttccggt acatccaaca ggacatggtc tttaagattc agataaggaa
    14821 acatcggcca atttttttgg atgatcgcaa aattatccag ttttgcaaca agaacctctt
    14881 ttttcaagtg ctccaatatt ttatgctgtt tttcgagagg acacaaaata acggtcaagt
    14941 gttcagcatg aatcaaatat tcaggtttca ttacgtccag gcctcctact ttgcaaatga
    15001 tggataaaaa aagtgaaagg gaatagacta agaatgagac agcatccact gagcccaata
    15061 agagtttggg caaatccctt aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg
    15121 gtaaaaaaag tctgttgatc catcggaatt gtaagtccgc ttaccgaagt caagatatca
    15181 cggatatctg gcagttcagt agtctttaag acaagaaaat tgatcctttg aagaagcgct
    15241 tcataaactg actggaagct tacgatccac aataaaacca agatacaaca aagaaagaga
    15301 ggaaagaaca gctgccagaa aataaaaagg aataatttgg ttttagaaag accagtcagg
    15361 cgccaattga tcaattcctg cttgttttgc ctgactgaat agtaaaagaa aaagataaaa
    15421 aggaaggcaa atagaaaaaa taaactgaat atgcctttta tataaaatcg gctagctaat
    15481 tctaaagccg gaagaagctc tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg
    15541 acttgattgg ctaaagccga ttgcaaatgg taaagagtca ggcatgctat caataaaaaa
    15601 gcagaaaagc ttgaaaataa acaatagagt attgtctgct ttttgtaata aattaatcga
    15661 gcagtagcat agcggattat tttcatactt ttcacctcta taaaaagtat agaaaatgaa
    15721 tatgaataaa gtatgaaatt cctttagtga aatcacgaat taaaaccaga aaatattttg
    15781 taaaaagcaa gaggttgtga agaagctgcc ctattctatt accgtagatg caagcttttg
    15841 aacactgttt attcgaaaag aggagcagcg acaataattt tgtcgctgct ccttttaaat
    15901 ttattttgtc attcggaaga acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt
    15961 ttataaagat caacagttga ttgtccgttg tgttcactaa agacactagt tgacttgtca
    16021 ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc ataatcactg
    16081 gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc attattgatt
    16141 tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat ttcattctgt
    16201 acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat cgtaccaccg
    16261 tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag ctgttcatga
    16321 tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac acctagtaag
    16381 tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat tccgccctgt
    16441 gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt gtcgtagttt
    16501 gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct tgaattcgag
    16561 acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc gctttctttc
    16621 aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc aaagtaaccg
    16681 ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt ggtgaatttt
    16741 gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg ttgcagacgt
    16801 tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa
    16861 ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc taaattcttg
    16921 tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc actagtgtaa
    16981 ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc gcctaattgt
    17041 gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt ttccgcatcg
    17101 cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg
    17161 tggtaaagac tattgatgaa aggtgtaact tcatgctgga gaccattctc atctgtcaat
    17221 ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac attttttcct
    17281 ttagcgagcc cgaacaaatc gtcatttggt gctgcataat gttcttttac ataattttct
    17341 acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg gtaagtttga
    17401 acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata atcacgagag
    17461 aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt tcctgagaaa
    17521 atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc
    17581 aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat ccaatagatc
    17641 acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc tactttccca
    17701 gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata agtgacattc
    17761 gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata agcaactcta
    17821 gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat agggttgata
    17881 aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac taaataggca
    17941 aaaatcgttt tcaaccaaag taaaacgact agtaaagaaa aaaatccgag cctcttattc
    18001 aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact tcttaatcac
    18061 agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt actagtatga
    18121 caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt tctttaagct
    18181 tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg ggaagagaaa
    18241 gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa
    18301 aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa
    18361 tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag
    18421 aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca atcttttttt
    18481 gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga tgattctact
    18541 accgcttttc gtatattcaa tggagaagga gacggaatcg gcggtctgat tgttgattat
    18601 tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta
    18661 ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga gaaaattcga
    18721 tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc accggaaccc
    18781 ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg attaatgact
    18841 gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt cgcttctggg
    18901 aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc cgcaatggga
    18961 ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa
    19021 cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc
    19081 gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt attggatcca
    19141 ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta tggggaactg
    19201 gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc tacaaatgct
    19261 gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca tgccaaaaat
    19321 gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac gacggatacc
    19381 tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag
    19441 gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag accaaagatt
    19501 tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt tcttgcacgt
    19561 gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt tttttctttt
    19621 gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact gccgttatta
    19681 tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc
    19741 gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat cgagttgttc
    19801 aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa cattccttta
    19861 attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct ttaccgattg
    19921 aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc caataatgaa
    19981 cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt ctccatgcct
    20041 attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg cggaacgacg
    20101 ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc aggacaatta
    20161 ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta gaacaagttt
    20221 gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga
    20281 acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc acctaagtgc
    20341 gtcatcggtc gctgaacagc ttccagaaag ccaatctcgt atatttaaaa cactggttgc
    20401 tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg aattgaatct
    20461 aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt taaaagattt
    20521 ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga aaaaactatt
    20581 tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct cggctggcag
    20641 aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga gcggacaatt
    20701 tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg gatgttgttt
    20761 gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt cattcctgct
    20821 acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc attgacatcc
    20881 agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt tttaggataa
    20941 atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg aaaagttttg
    21001 ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg tttttgagag
    21061 gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac taacaatcgc
    21121 tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt acttttttct
    21181 gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc
    21241 actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg cagtgcagcc
    21301 ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt caatacacga
    21361 ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg aagtgttttc
    21421 tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag aggcgtcacc
    21481 gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt agatggataa
    21541 gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa ttccgtgcgt
    21601 ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga gaatagaagc
    21661 gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt tgatttattt
    21721 gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg tgattcaaag
    21781 aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa ctttccattt
    21841 ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt gcaccgacca
    21901 ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt
    21961 gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc gccgactacg
    22021 atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat atctgaagaa
    22081 agaaaacaga aaaaaaatgt tttgagccaa aagagaaaat gagctaaaat gagggaagat
    22141 aagcaaagga gcgtggaacg atgacaaacg caacagaata tgttcaagca attcaagaaa
    22201 agctacatca aaaggatcaa gggcaagaag aatttttaca agcaatcgat gaattcatgc
    22261 ccacggttat gtcttttttg gacactcatc ccgaatatat cgaaaagaat atcttagaac
    22321 ttttaacgga accagaaaga gttattcaat ttcgagtgcc ttggcaagat gatgaaggaa
    22381 actggcgagt aaatagaggg taccgtatcc aatacaactc agctatcggc ccttataaag
    22441 gtggattgcg cttccatcct agtgtcaatc taagtatctt gaaatttttg gcttttgaac
    22501 agattttcaa gaatagttta accggtctgc caatcggtgg aggaaaaggc ggaagtgact
    22561 tcgatcctaa aggaaaatct gataatgaga tcatgcgttt ttgtcaaagt ttcatgctgg
    22621 aattgtcaaa acatatcggc ccatcgattg atgttcctgc gggagatatc ggtgtaggag
    22681 ctagagaaat cggctatttg ttcggcgaat ataaacgatt gaaaaaatat gatactggtg
    22741 tgttgacagg taaacctttg gatttttggg gaagtaagat acgtacggaa gctactggtt
    22801 atggattagt gtattatgtc aaacatctat tgaacgaaga aaaagattca tttaatcaaa
    22861 aaacagtttt tgtttccgga agcgggaatg tagccattta tgcaatcgaa aaagtgcaag
    22921 agctaggcgg gaaagttgtt acatgttcag attctagcgg gtacatctat gatccagaag
    22981 gaatagatgt gaaactattg aaagaagtca aagaaatcaa tagacaacgt ttgacggaat
    23041 acgcaaacca acgtccttct gctatttatc atgctggaga atctgtttgg acattgaaag
    23101 aaaaagcgga tatcgctttg ccatgtgcta ctcaaaatga aatagacgaa agcttggccg
    23161 aaatcttagt agaaaatggg atcaaaatcg tagcagaagg cgctaatatg ccatgtacag
    23221 taaaagctgt ttctgttctt catgaagcag gcgtgtggta ttgtccagga aaagctgcta
    23281 atgctggagg agtagcagta tctgctttag aaatgagtca gaacgtccag cgattgactt
    23341 gggaaagaga gcaagtggat gagcagctgg atcaaatcat ggaaacgatt taccatacgt
    23401 gtcgccaaac agcagccgag tacggcgacg aaaaaaatct gcttcttgga gcaaatgttg
    23461 ctggatttga aaaagttgcc aaagcaatgg cagcagaagg tttagtttga aaaatgaaac
    23521 tttcttggaa atattgtcag cattttgttt atttgcttga aaaataataa aaaaaaacgt
    23581 aaagtattac ctgtagagta aacctataag gaggagaaca aatggcacac attcattttg
    23641 actattcaaa agtagcaccg tttgtcaacg aacaagagtt agggtacatg caaagccaag
    23701 taacagcagc ccacaacgaa ttacgggaag gtactggcgc aggaagcgac ttccgcggct
    23761 ggatcgattt accaacgaac tatgacaaag aagaatttgc acgtatcaaa gaagcagcta
    23821 aaaaaatcca atctgattct gaagtattag ttgttatcgg tatcggtggt tcttatttag
    23881 gcgctcgtgc agcgatcgac ttcttgaatc acacattctt caatttatta gatagcaaca
    23941 caagaaaagc accacaaatc ttctttgccg gaaattcaat cagctcaaca tatattgctg
    24001 acttgatcga agtaatcggt gatcgcgact tttcagtaaa cgttatttca aaatctggaa
    24061 caactactga accagcgatt gcgttccgtg tattcaaaga gcttcttgtt aagaaatatg
    24121 gacaagaaga agcaaacaaa cgtatctacg ctacaacaga taaagcaaaa ggtgcagtta
    24181 aagtagaagc agatgcagaa ggttgggaaa ctttcgtgat tcctgatgac atcggcggac
    24241 gtttctctgt attaactgct gttggtttgt taccaatcgc tgttagtggt gcagatatcg
    24301 atgcattgat gcaaggtgcc gctgatgcaa gcaaagcata ctcaagcgac aaactagaag
    24361 aaaacgaagc ttaccaatac gcagcaatgc gcaacattct ttaccgtaaa ggcaaagtaa
    24421 ctgagttgtt gatcaactat gaaccaggaa tgcaatactt ctcagaatgg tggaaacaat
    24481 tgtatggtga atctgaagga aaagaccaaa aaggcatcta tccttcaagc gcaaacttct
    24541 caactgattt gcactcttta ggacaattca tccaagaagg acgtcgcaat atttttgaaa
    24601 cagttgtgaa ggttgaaaaa cctcgtaaat caatcacaat tcctgaacaa gcagaagatt
    24661 tagacggtct aggctacttg caaggaaaag aagtggactt cgtaaataca aaagcatttg
    24721 aaggaacatt gcttgctcat acagacggtg acgtgcctaa cttgctagtc aaaattccag
    24781 aaatggatgc ttatacattg ggttatacaa tgtacttctt tgaaattgct gtaggtattt
    24841 caggttactt gaatggaatc aacccattcg accaaccagg tgtagaagct tacaagaaaa
    24901 acatgttcgc acttctaggc aaaccaggct ttgaagatct tgctaaagaa ttgaacgaac
    24961 gcctgtaaga taagggtttt tcgctagatt taaataatca gtgatagaaa catgttcgct
    25021 ttaaaatgaa aactggtacc catttaggta ctcaaacata ctgcatcgtt tattcagaat
    25081 aaggggctgt gacaagacgt ttgtcacagt cccttgtttg tatagaaatc gattgattgc
    25141 cttgctcttt agtttgtttt aggactttaa gaacagtgag gctgacttcg aaggttaatt
    25201 ctgttttttt gttttctaga atcagtaagc aaaaaagcac tcacttcgag tgcttttttg
    25261 cgatatattc ttagcttaag ataggcctaa tttttctttt acagatgagg gtaccttatc
    25321 gctggtaatt acttttttct ctatgatccg cttttcgctt gctttcaact gcaagatcgt
    25381 tccttcgggg taagcagaac cattggcaga gtcaccagaa gtaatgacat cgaaatctaa
    25441 ctgtcttttt tcaccgtttt cagcataagc atctacgttg tatttaaaac ctcttccaat
    25501 tacttcccca ttatctgctt tgatatcagt ttctgcaggc attggagctt tgactaccgc
    25561 ataataatct ttacctacat aagtagactg atagtagtcc caacctttcc atcctataaa
    25621 agcaatcaat atgataacga tcaaccctaa aatttttttc atgaaagatc tcctttttta
    25681 tttttatta
//
LOCUS       DAJSAW010000036        24383 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJSAW010000036 DAJSAW010000000
VERSION     DAJSAW010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.036"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(53..901)
                     /locus_tag="ONT64_001713"
     CDS             complement(53..901)
                     /locus_tag="ONT64_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2303129.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1125..3065)
                     /gene="thrS"
                     /locus_tag="ONT64_001714"
     CDS             complement(1125..3065)
                     /gene="thrS"
                     /locus_tag="ONT64_001714"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR2303130.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3402..3581)
                     /locus_tag="ONT64_001715"
     CDS             complement(3402..3581)
                     /locus_tag="ONT64_001715"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCR2303131.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3628..5451)
                     /gene="cadA"
                     /locus_tag="ONT64_001716"
     CDS             complement(3628..5451)
                     /gene="cadA"
                     /locus_tag="ONT64_001716"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCR2303132.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5729..8008
                     /locus_tag="ONT64_001717"
     CDS             5729..8008
                     /locus_tag="ONT64_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2303133.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8101..8748)
                     /locus_tag="ONT64_001718"
     CDS             complement(8101..8748)
                     /locus_tag="ONT64_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303134.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8927..9646)
                     /locus_tag="ONT64_001719"
     CDS             complement(8927..9646)
                     /locus_tag="ONT64_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2303135.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9777..10928)
                     /locus_tag="ONT64_001720"
     CDS             complement(9777..10928)
                     /locus_tag="ONT64_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCR2303136.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11145..11885)
                     /locus_tag="ONT64_001721"
     CDS             complement(11145..11885)
                     /locus_tag="ONT64_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR2303137.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11889..12212)
                     /gene="rsfS"
                     /locus_tag="ONT64_001722"
     CDS             complement(11889..12212)
                     /gene="rsfS"
                     /locus_tag="ONT64_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR2303138.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12269..12862)
                     /gene="yqeK"
                     /locus_tag="ONT64_001723"
     CDS             complement(12269..12862)
                     /gene="yqeK"
                     /locus_tag="ONT64_001723"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCR2303139.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12846..13496)
                     /locus_tag="ONT64_001724"
     CDS             complement(12846..13496)
                     /locus_tag="ONT64_001724"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR2303140.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13506..13817)
                     /gene="yhbY"
                     /locus_tag="ONT64_001725"
     CDS             complement(13506..13817)
                     /gene="yhbY"
                     /locus_tag="ONT64_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR2303141.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13833..14942)
                     /gene="yqeH"
                     /locus_tag="ONT64_001726"
     CDS             complement(13833..14942)
                     /gene="yqeH"
                     /locus_tag="ONT64_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCR2303142.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14939..15466)
                     /locus_tag="ONT64_001727"
     CDS             complement(14939..15466)
                     /locus_tag="ONT64_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCR2303143.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15646..16431)
                     /locus_tag="ONT64_001728"
     CDS             complement(15646..16431)
                     /locus_tag="ONT64_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR2303144.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16424..17293)
                     /locus_tag="ONT64_001729"
     CDS             complement(16424..17293)
                     /locus_tag="ONT64_001729"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCR2303145.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17294..18673)
                     /locus_tag="ONT64_001730"
     CDS             complement(17294..18673)
                     /locus_tag="ONT64_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR2303146.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18681..19100)
                     /gene="fabZ"
                     /locus_tag="ONT64_001731"
     CDS             complement(18681..19100)
                     /gene="fabZ"
                     /locus_tag="ONT64_001731"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR2303147.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19125..19601)
                     /gene="accB"
                     /locus_tag="ONT64_001732"
     CDS             complement(19125..19601)
                     /gene="accB"
                     /locus_tag="ONT64_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR2303148.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19604..20839)
                     /gene="fabF"
                     /locus_tag="ONT64_001733"
     CDS             complement(19604..20839)
                     /gene="fabF"
                     /locus_tag="ONT64_001733"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR2303149.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20858..21595)
                     /gene="fabG"
                     /locus_tag="ONT64_001734"
     CDS             complement(20858..21595)
                     /gene="fabG"
                     /locus_tag="ONT64_001734"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCR2303150.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21607..22524)
                     /gene="fabD"
                     /locus_tag="ONT64_001735"
     CDS             complement(21607..22524)
                     /gene="fabD"
                     /locus_tag="ONT64_001735"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR2303151.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22631..22855)
                     /locus_tag="ONT64_001736"
     CDS             complement(22631..22855)
                     /locus_tag="ONT64_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR2303152.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22908..23870)
                     /locus_tag="ONT64_001737"
     CDS             complement(22908..23870)
                     /locus_tag="ONT64_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCR2303153.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23880..24320)
                     /locus_tag="ONT64_001738"
     CDS             complement(23880..24320)
                     /locus_tag="ONT64_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2303154.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6672 a         5429 c         4248 g         8034 t
ORIGIN      
        1 gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt ttttatttcg
       61 aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg ctaagctttt
      121 cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg
      181 aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt acgagtacat
      241 cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt tcgatattca
      301 tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag tttgaaacaa
      361 gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg tggatatctt
      421 tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct tctactacta
      481 aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa cactctacga
      541 ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct acttgtgcat
      601 tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata tcactaaaac
      661 cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc gtcagacttt
      721 gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg
      781 ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg tagttggcca
      841 actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta attacgctca
      901 taagttcctc caccctttta atgatagtct atgtaagtat accatatcag acccttattc
      961 acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata tcttatccca
     1021 tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt atctattgtt
     1081 cggattgctt tctatctact acttttttac aggaaattta cctattagtt ttctctgctg
     1141 taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc tacagctgtt
     1201 tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt gtctccaaca
     1261 actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc cattttttca
     1321 ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt gatttcataa
     1381 gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat aggtgccaac
     1441 catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg ttccattgta
     1501 gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc ttctccaaca
     1561 tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga taatgtttct
     1621 tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg
     1681 ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag cattgcttgg
     1741 gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg atcgcgatag
     1801 ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac catcaattca
     1861 agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg accatcattc
     1921 aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc atagcgatgc
     1981 atcattccta attcagcgat acgaattggt aattcacgat aagaatggat cgtgtttttg
     2041 tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc gccatctccc
     2101 atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga tgttttgtac
     2161 aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact aacttctttg
     2221 tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag ccagaatggc
     2281 aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc tagtttacga
     2341 tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc tttcttgtca
     2401 aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc acgccaataa
     2461 gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg aacatgtggt
     2521 ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc ttcttctggc
     2581 aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt
     2641 tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat catttctgct
     2701 tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt atcgtagtaa
     2761 aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag gcgacgcatg
     2821 gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc ttcatggtct
     2881 ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc aacgagtttt
     2941 ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct ttcagcaatt
     3001 gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa agtaattttt
     3061 atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa
     3121 aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg ttccacctaa
     3181 tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca
     3241 agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc ctagcttttc
     3301 ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt atgtgtttat
     3361 ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt ccattcccca
     3421 tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg ttcttttggt
     3481 gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc taattgttcc
     3541 ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc tcctctttct
     3601 gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta ctgaatcagg
     3661 aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg tatccacgac
     3721 ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa ttaacatcaa
     3781 aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca tcgtatctct
     3841 tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga ccgcatctgc
     3901 actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat ctgcagcagc
     3961 aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt gatctttagg
     4021 gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta cagaatctaa
     4081 ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga tcatcatcaa
     4141 atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg ggcgaatctg
     4201 atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt
     4261 ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt
     4321 attgactact gcttggattc cttgaccggt cacttctttc agttgactaa caggatacag
     4381 ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag attcctgttc
     4441 tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg gctgaatcgc
     4501 ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt
     4561 acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt tacttgaacg
     4621 gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag gtgatgccac
     4681 taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa taccaccgat
     4741 caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga caaatcgggc
     4801 aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc tgtctgcggc
     4861 acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg acataatctg
     4921 atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt catcgaccga
     4981 agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct tgactagtaa
     5041 ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt ctttgaatcg
     5101 atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg ctcgtttatt
     5161 cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca aactagccca
     5221 gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat ctacgccata
     5281 tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga gaatccctcc
     5341 agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt cacttaataa
     5401 agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca ttgttttttc
     5461 tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc tttcatctta
     5521 agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa caactatcac
     5581 tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa ggattacttt
     5641 ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact tttaattcgt
     5701 tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac aaacgcacgt
     5761 ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa aacaacaaga
     5821 attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga ttcgagctgg
     5881 ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc aggaattgct
     5941 tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa
     6001 agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag aaacattata
     6061 tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg actggcgcgc
     6121 accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg aaactgaaac
     6181 agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag aaggacgtat
     6241 tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag agatattgga
     6301 tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc
     6361 aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa
     6421 gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca agtggctcga
     6481 ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca tctcgactgt
     6541 attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca
     6601 gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt ttttgctagg
     6661 ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc agattgatcg
     6721 ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa taaatggccg
     6781 tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg aacaattacc
     6841 tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag gcggtctgca
     6901 aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc tccaagaact
     6961 atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa
     7021 actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta
     7081 tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc cactttctct
     7141 tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa ctgttcgtac
     7201 acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt tactgatgcg
     7261 ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg atgaaatgca
     7321 ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag caaatttgac
     7381 tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg gatcattgga
     7441 agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct atcgctccac
     7501 aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag tagaaacaac
     7561 agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata tcaaattgaa
     7621 ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc gtacagcaat
     7681 catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag acgagttgaa
     7741 acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca ttattttact
     7801 tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc
     7861 agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta cacgtgcgat
     7921 gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca atatggatat
     7981 ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat taatgttagc
     8041 agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat ttaaggtcat
     8101 ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa gcgaaataat
     8161 cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc tatgacggcc
     8221 aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt ggtcaattga
     8281 ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac gaaatgctag
     8341 gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc tttcgataag
     8401 aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag cctccatccg
     8461 atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca gcggaatcag
     8521 ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta aataacggtt
     8581 cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga atcttgcgta
     8641 tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta ctttgcggta
     8701 accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat tcttccccgt
     8761 ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc ataccacagg
     8821 ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta gtgagattgt
     8881 cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag tcttctaatg
     8941 ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca atcatttttt
     9001 caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc atttggatct
     9061 cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata ctatggaaat
     9121 ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg cgaacatcga
     9181 tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga
     9241 ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc ccgccatttt
     9301 tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc aatgtatcaa
     9361 caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag tatgtttcat
     9421 ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt acattataag
     9481 ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca ggatcacctg
     9541 attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta ttattagcat
     9601 ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt tcacttcgct
     9661 ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg
     9721 attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc aattatctat
     9781 actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa ttgatagatt
     9841 tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat tcgggaataa
     9901 atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa tccaagtagt
     9961 cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt cagcaagaca
    10021 taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac ctttgacaca
    10081 aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc tattccttcg
    10141 gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg ataacgcaaa
    10201 tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc ttctgtctga
    10261 ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat ggctgttgca
    10321 ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt acgtcggatc
    10381 ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact taatcccaag
    10441 atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg aaaaacctca
    10501 gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc ctcttctatc
    10561 ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga agtctcttct
    10621 tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac tgcaccttta
    10681 gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac taaatccaca
    10741 ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg ttcgcctctt
    10801 tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat ttttcgtgcc
    10861 atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat aatcccgcag
    10921 cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga caagtaaaaa
    10981 aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa cacaatactt
    11041 ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat ataatttatg
    11101 ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc tttgtatgct
    11161 acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct
    11221 tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa tggataggta
    11281 cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg ttcttcaacg
    11341 aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact atcccacaaa
    11401 aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc atctacttga
    11461 taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg atagacttca
    11521 tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc agaaaaacag
    11581 gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc cacaaattgg
    11641 atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg
    11701 tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg
    11761 catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac
    11821 cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa
    11881 gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga ccatagtttt
    11941 tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac atctccaaga
    12001 tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc atatttgttt
    12061 tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc gctatttgct
    12121 gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa agcgataatg
    12181 tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc tttactatct
    12241 atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa ttatatgttt
    12301 cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat gtatgttttg
    12361 tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca tcttgtaccc
    12421 caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg tcaaggagac
    12481 tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct gcatcggtga
    12541 ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt gcattattat
    12601 aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac tcctcatctg
    12661 gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca ggagaagcac
    12721 cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc ttgaagcgtt
    12781 gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa gcagtatatt
    12841 cattactata gtccatattc atacaaccct tcattttgaa tataatcaat tactttatct
    12901 ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt cgtggaactg
    12961 atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt tgtgtatcct
    13021 ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt ataccatttt
    13081 ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc cggattgttt
    13141 tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc taattcaatc
    13201 gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt gagtcgatgt
    13261 tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg catcaaaaat
    13321 acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg tgcaaggtga
    13381 acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa gaattgaggc
    13441 atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt catcatggtc
    13501 actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt tgtggaaggt
    13561 ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat ttctttttct
    13621 aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga tactttgatc
    13681 agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt gatcccacct
    13741 tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt
    13801 tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg tttcaataca
    13861 tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt gatccagcct
    13921 agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg aacaagctcc
    13981 ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac atgtttttga
    14041 taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt tgacacataa
    14101 gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa
    14161 agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa cggtgcaatg
    14221 atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat aattcccggt
    14281 gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct
    14341 ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat gatttgattg
    14401 ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg tccatcgcgg
    14461 tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc actagttaat
    14521 aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat
    14581 ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa aagtactgga
    14641 ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc aaaaatatcc
    14701 accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg caaaaagtcg
    14761 tcatccgtca atgagacatc ttgggtctca ttataatggc gtaatctgaa acaacgctga
    14821 caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt gtatcctaat
    14881 tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa ttcttctgtc
    14941 attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag gccataattc
    15001 ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata atgggtttta
    15061 caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata atctgatcgc
    15121 ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg ttgatccctc
    15181 ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca atcgctcgcg
    15241 cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca tttttcattt
    15301 ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca atcaacgtat
    15361 tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca ggtgtaatct
    15421 gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg tctcctcatc
    15481 tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact gcatatcagc
    15541 aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac
    15601 caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat attggcggaa
    15661 cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct
    15721 ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact gctcgccgtt
    15781 gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg gcgcagtaat
    15841 tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa
    15901 tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt catccgctaa
    15961 acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga tgatcggtac
    16021 ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc cgcgctcttc
    16081 tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg gtctattgaa
    16141 cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc
    16201 aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga tcactgttac
    16261 cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg caaataaacg
    16321 atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca agcttgtcca
    16381 tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca cgatctatcc
    16441 atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat tccatgcggg
    16501 gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg aaatcttctg
    16561 gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt
    16621 ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact cctccggtcg
    16681 tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt ttgacagcag
    16741 cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt gcgccacctg
    16801 aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat aatctggtga
    16861 tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc atcactccaa
    16921 taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca tgaagtcctg
    16981 ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc ggatctttgg
    17041 ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa gctagacgct
    17101 gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct tcaccgatgt
    17161 ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta tcaggaacag
    17221 atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat ttctttttct
    17281 taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt
    17341 cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat
    17401 ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt aaggctcgct
    17461 gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt gcaatcattg
    17521 aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact cgcagtccca
    17581 tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca aaatggaaag
    17641 caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact acttcttctt
    17701 gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact aaatcgattc
    17761 ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt tccatgaaat
    17821 aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca taattgactg
    17881 ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt gagatcacta
    17941 cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa tctcgctcgc
    18001 ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata tggcgagcag
    18061 gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct gcttcttgct
    18121 gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga atgcctttcc
    18181 cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct gctatttcca
    18241 atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg acatttgctt
    18301 cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt gcacttttag
    18361 gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta ttttcagata
    18421 aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg ctcagtactt
    18481 cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt gcttcatctg
    18541 ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg gtttggatcc
    18601 ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta gctatcaata
    18661 ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc ggctactttt
    18721 ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac tttcaatatc
    18781 tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt tgctttgtcc
    18841 aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa tgcaactgct
    18901 cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc ttctggaaaa
    18961 tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat ccgttctcct
    19021 tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg aatgatttct
    19081 tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt ttgctgaatc
    19141 ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat ttctgtgatc
    19201 actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat aatgcagata
    19261 acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc gggcgctggt
    19321 tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa tactgccgat
    19381 tctttttctt tcattatttc tggagcagtt gttgcttgtt caggtatgac attgactggc
    19441 tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact tgttttattt
    19501 ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag cgaggattga
    19561 tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc tcccagcgtt
    19621 tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt gcataagtaa
    19681 aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga tcttgttttt
    19741 ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt gccaaggctt
    19801 cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag
    19861 tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc tggtcattgg
    19921 ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt acaccagctt
    19981 cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta ggagctgtca
    20041 tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt ttggcaccgc
    20101 gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca cccatcacaa
    20161 aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt gattctgaga
    20221 gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct tctgttcctc
    20281 cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc ccaattgaat
    20341 tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca cctacacgca
    20401 aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt ggagaaactc
    20461 gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga agaccaccga
    20521 tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta tctaaaccgc
    20581 tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata tccattcgtt
    20641 tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca cccgctaagg
    20701 taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc gtcccctctt
    20761 ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact ccatagccag
    20821 taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg accaatccgc
    20881 catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa actgctgttt
    20941 gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct
    21001 tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc gcaatggcat
    21061 tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat cctactacgc
    21121 cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca gatacactag
    21181 agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg tgctgcgtca
    21241 tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct gcatccattc
    21301 gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg atcgatccaa
    21361 gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag atgtcaccgg
    21421 aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct tttggaatct
    21481 ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag gccatcgctt
    21541 tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca tccataacgt
    21601 tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac
    21661 tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg ggccgacttc
    21721 gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga aacgaacagg
    21781 ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta tttccgttgt
    21841 tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta ggtccttcgc
    21901 cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga catttagagg
    21961 gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc
    22021 ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata cgatgccgta
    22081 cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca tcactgcaac
    22141 cattttcccg cttccagctg gggcagcttc tgtcatataa gcgcctcttt ttgctactaa
    22201 cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat attctcctaa
    22261 actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc gataatacgc
    22321 gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac gttcattttc
    22381 tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat caaatgtctg
    22441 ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg caccttggcc
    22501 actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat atgtttgatt
    22561 cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc ccccaaccta
    22621 attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga accgttttga
    22681 ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg atgatttgga
    22741 ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat ttgatttctt
    22801 cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat accataataa
    22861 tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta caactccatg
    22921 agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt ctgctgtttt
    22981 cctaaggtga tctttccact agaaactgct tcatccaata agatagggat gcttgcagca
    23041 gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat ctttactttt
    23101 cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata atcaactggt
    23161 ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa atcaaaaata
    23221 tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata gaacggattt
    23281 ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc tgctaatttt
    23341 tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc tccatcgcca
    23401 aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt ttcaccacca
    23461 atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct taacgcataa
    23521 acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc tcccaatttc
    23581 ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa
    23641 ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc cgccactaaa
    23701 atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat acccgtcctt
    23761 gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt agtaataatc
    23821 ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat taaatatcct
    23881 cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt
    23941 tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa atgctgatgg
    24001 acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg gcggtcatct
    24061 tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc agtggtcaag
    24121 gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt cttgtacatc
    24181 ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg agattttttc
    24241 agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata atcatttact
    24301 gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat caaatctttt
    24361 gatgttcaaa ctatatctta act
//
LOCUS       DAJSAW010000037        23831 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJSAW010000037 DAJSAW010000000
VERSION     DAJSAW010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.037"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(55..789)
                     /locus_tag="ONT64_001739"
     CDS             complement(55..789)
                     /locus_tag="ONT64_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCR2303155.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="ONT64_001740"
     CDS             1116..1526
                     /locus_tag="ONT64_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR2303156.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="ONT64_001741"
     CDS             1519..2220
                     /locus_tag="ONT64_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCR2303157.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="ONT64_001742"
     CDS             2220..3806
                     /locus_tag="ONT64_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2303158.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="ONT64_001743"
     CDS             3825..4046
                     /locus_tag="ONT64_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCR2303159.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="ONT64_001744"
     CDS             4043..4804
                     /locus_tag="ONT64_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HCR2303160.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="ONT64_001745"
     CDS             5184..5693
                     /locus_tag="ONT64_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCR2303161.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="ONT64_001746"
     CDS             complement(5768..6307)
                     /locus_tag="ONT64_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCR2303162.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ONT64_001747"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ONT64_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR2303163.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="ONT64_001748"
     CDS             7320..8171
                     /locus_tag="ONT64_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR2303164.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="ONT64_001749"
     CDS             8199..8834
                     /locus_tag="ONT64_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HCR2303165.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="ONT64_001750"
     CDS             8854..9627
                     /locus_tag="ONT64_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2303166.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="ONT64_001751"
     CDS             10035..10832
                     /locus_tag="ONT64_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCR2303167.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="ONT64_001752"
     CDS             10810..11223
                     /locus_tag="ONT64_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR2303168.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="ONT64_001753"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="ONT64_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR2303169.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="ONT64_001754"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="ONT64_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR2303170.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /gene="maf"
                     /locus_tag="ONT64_001755"
     CDS             16000..16560
                     /gene="maf"
                     /locus_tag="ONT64_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HCR2303171.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ONT64_001756"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ONT64_001756"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCR2303172.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="ONT64_001757"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="ONT64_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCR2303173.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="ONT64_001758"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="ONT64_001758"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR2303174.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ONT64_001759"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ONT64_001759"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR2303175.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ONT64_001760"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ONT64_001760"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR2303176.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ONT64_001761"
     CDS             21907..22485
                     /locus_tag="ONT64_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2303177.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ONT64_001762"
     CDS             22537..23019
                     /locus_tag="ONT64_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCR2303178.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ONT64_001763"
     CDS             23136..23510
                     /locus_tag="ONT64_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303179.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7783 a         4285 c         5114 g         6649 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt t
//
LOCUS       DAJSAW010000038        23485 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJSAW010000038 DAJSAW010000000
VERSION     DAJSAW010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.038"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            2..74
                     /locus_tag="ONT64_001764"
     tRNA            2..74
                     /locus_tag="ONT64_001764"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ONT64_001765"
     tRNA            78..150
                     /locus_tag="ONT64_001765"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ONT64_001766"
     tRNA            155..225
                     /locus_tag="ONT64_001766"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ONT64_001767"
     tRNA            238..311
                     /locus_tag="ONT64_001767"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ONT64_001768"
     tRNA            323..394
                     /locus_tag="ONT64_001768"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /locus_tag="ONT64_001769"
     CDS             660..1853
                     /locus_tag="ONT64_001769"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR2303180.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ONT64_001770"
     CDS             1983..3455
                     /locus_tag="ONT64_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2303181.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ONT64_001771"
     CDS             3538..4326
                     /locus_tag="ONT64_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR2303182.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ONT64_001772"
     CDS             complement(4393..5061)
                     /locus_tag="ONT64_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HCR2303183.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ONT64_001773"
     CDS             5176..6888
                     /locus_tag="ONT64_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2303184.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8660
                     /locus_tag="ONT64_001774"
     CDS             6891..8660
                     /locus_tag="ONT64_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2303185.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8865..9431)
                     /locus_tag="ONT64_001775"
     CDS             complement(8865..9431)
                     /locus_tag="ONT64_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2303186.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9431..10387)
                     /locus_tag="ONT64_001776"
     CDS             complement(9431..10387)
                     /locus_tag="ONT64_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR2303187.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(10387..11043)
                     /locus_tag="ONT64_001777"
     CDS             complement(10387..11043)
                     /locus_tag="ONT64_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR2303188.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11413..13431
                     /locus_tag="ONT64_001778"
     CDS             11413..13431
                     /locus_tag="ONT64_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2303189.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    13501..13707
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13858..16272
                     /locus_tag="ONT64_001779"
     CDS             13858..16272
                     /locus_tag="ONT64_001779"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR2303190.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            16620..18173
                     /locus_tag="ONT64_001780"
     CDS             16620..18173
                     /locus_tag="ONT64_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCR2303191.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(18554..20338)
                     /locus_tag="ONT64_001781"
     CDS             complement(18554..20338)
                     /locus_tag="ONT64_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2303192.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(20353..22074)
                     /locus_tag="ONT64_001782"
     CDS             complement(20353..22074)
                     /locus_tag="ONT64_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2303193.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            22174..22935
                     /locus_tag="ONT64_001783"
     CDS             22174..22935
                     /locus_tag="ONT64_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR2303194.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7428 a         4213 c         4869 g         6975 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattatttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaactgt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcacgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaataa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
     8701 cagccaatca aaaacagctt ctcatgtttt tgatttggtt ggacttatta ccgcagatat
     8761 aagcttttga gcaccgtgaa taaagaggtt atgaaatcaa cgctctaacc tgagtattag
     8821 agaaaaaaac gcaaaacata tcatttatgt tttgcgtttt ttatttatct ttttttctca
     8881 atacaatata aaataggcgg ctggttcttt tggttgataa accgataatt cagcacatta
     8941 tatgtttcct gtggcagttt ggaacaaaaa gaatcaacca tatccagttc tttttctcca
     9001 ccttcatgac catagtagac gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat
     9061 atttcttcca tagctgtgcg tgtagtctct ggcatagtga tgattgcttt atcacttttt
     9121 gggagatacc ctagattgaa aatccctgct ttgagatttt gttgttcagc tatcacttct
     9181 cctaacgttt cgtggccttg tagaaacaat gagacacgtt cttctaactg acgctgttct
     9241 agcctttcac gagtagcatc gattgcttgt tgctggatat caaaactata gacatgacca
     9301 gtttttccga tgtgttctgc tagaaaaacc gtatcgtggc cattgcccat cgttgcatct
     9361 acgacgtgat cccctggttg caagatttct tgcagtaatg tatggctgaa atgcaaagca
     9421 gtttgcaaca tcagacgctc acctttttcc gtacatcata ttttccttgg aagctatctc
     9481 tgcgaagaag ttcttcatca attgcattca agacttccca ttttttcaag ctccacattg
     9541 gtccaatcaa ggaatcccac ggcgcatccc ctgtcaaacg atggatgatg atctcttttg
     9601 gtatcatttc cagctggtcg caaatgacat tgacatagtc aggacggctc attaattgaa
     9661 ggcgaccttc atgatagtcc cgcaacattc tcgttttgcg catcagatgc atcaaatgga
     9721 gcttgattcc ttggatatct gaatctaaaa tggtgcgtct gacattttct ttcatcattt
     9781 ctaaactttc gcctggtaat ccgttgatca aatgcgtaca aacacggata ttatgtttgc
     9841 gtaatttagc cacgccatct aaataagttt gataatcatg tgctcggttg atcagttttg
     9901 aagtttcttc aaaagttgtt tgcaatccaa gttctaccca taaatacatc cgctcgttca
     9961 gttcagcaag gtaatcaact acttcatctg gcagacaatc cggtcgtgta ccgactgata
    10021 atcctacgac acctggtaaa ttgactactt gctcaaatcg ttctttgatt atttcaactg
    10081 gcgcatgggt gtttgtaaaa ttttggaaat aaacgatgta ttgttggaca tgaggccatt
    10141 tttgatgcat catgtcgatt tctttctgga attggattgg aagagggtct tcaggtgcaa
    10201 cgatcatgtc tcccgaacca gatacactgc aaaaagtaca accgccatgg gcaacagtcc
    10261 catctcgatt cggacagtca aatcctccat cgatcggcac cttgaaaatt ttcccgccaa
    10321 actcatttcg taaagcgtaa ttccaagaat gatatctttt atttccttct gaataaggaa
    10381 aagtcattaa aagtccttct ttctgtaaga gttatctatt tgttttcttt tttctctata
    10441 cactggataa gtcaggcaca gccaagaaag actgagtaag taaccgccga tgatatcagt
    10501 aggaaaatga acgcccagat aaatgcggct tatcccgaca cttactataa taaacatcat
    10561 cagcaattga agcaatgtgc gtaaggttct tttttgaaca aaagctggaa gcagaaatag
    10621 taaagaaccg taaagaatca tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt
    10681 ttcaactact aagtgcgcca atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg
    10741 gttgatgatt ccagcaatta caataaatcc agaaacgaac caaaatgttt ctgccctttt
    10801 tttcttatac cacatccata aagccgcaca agcaaataaa atgatgacgg taagaggatt
    10861 accgaattta gtcataaaga gttggtagct gttcagatca ggataagaag ctctgacaat
    10921 gcctgtaaac cactgatcga aaccagaaag ccacgacaga tggaacttta cgatccagcc
    10981 caaaatgaaa aacaggacaa gaaagaagct accagccacc tgaaatgatg ttttatattt
    11041 catacaattc ctcctttgaa gcaatgactt attatagcat ttttggtcca gcgaaaaaaa
    11101 cggtaaagag ataaaaccaa tgtatgattc ctctttgaat aaccaatatt cttataaaaa
    11161 aaataactgt aatcggaaag acattttttg gtaatgcaaa tgaaatgtta gggaaataaa
    11221 atcagtgttt ttcgaaatcc ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg
    11281 aacagaagtt gttcattacg gtaaattaca atatgattac aaaaaacaac aaagctctta
    11341 aataactttt aataaatatt aagagaagtt ataattatta ataatagata aattgaagtt
    11401 ggaggataga agatggaaaa cattgcaaga aaagaacgga gacgtttaga acagaccaaa
    11461 cgttttcgaa aagcaaaacg aggggcagca atcgtgggaa ctgcgatggt aggctgttct
    11521 gtcgctgccc cattagtaca gccggttcct gttcaagcgg atgaaacacc tgttcagttc
    11581 ggcgcaagaa tcaatacagc ggcatttatc acagaaattg ctacttatgc tcaaccaatc
    11641 gctcaagcca acgatctata tgcatcagtg atgatcgcac aagcagttgt agaaagcggt
    11701 tggggcagta gtgcgttatc tcaagcacct tattataatc tttttgggat aaaaggcagc
    11761 tatcaaggac aaaccgtcta catggatacg cttgaatttt taaataatca atgggtaaat
    11821 aaaaaggagc ctttccgaca atatccttct tttgctgaat ctttcaatga caatgcctat
    11881 gtacttagaa atacttcatt tggcaacggt tattactatg ccggtacttg gaagagcaac
    11941 acgaaatcct atacagatgc gacagcttgt ttgacagggc gttatgcgac tgatcctggt
    12001 tacgcaggca aattaaataa tatcatcgca acttacggat tgacccaata tgatacacca
    12061 gctagcggaa acgcaggcgg tggagcgact acaggtggca ataatactgg aagtgcaggt
    12121 agtactggcg gttcaagcaa ttctggtaat acaaactcag gttcagcaac ttcgaccact
    12181 tatactgtcc aatctggtga ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg
    12241 gatcaattga tccaatggaa caacatccaa aacaatttca tttatccagg acaaaaactg
    12301 acaatcaaag gtagccagga aaatggctct tcgacgaata attccggtaa caataccaat
    12361 tcttcaggaa atgctggtac atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt
    12421 caatctggtg attctgtttg gaagatctca aatgatcacg gaattacgat ggctcaattg
    12481 attgaatgga acaatatcaa gaacaatttc gtttatccag gacaacagct aatcgtatca
    12541 aatggcggtt cttctaatgc tggagcaaat actggctcaa atactgggaa tacaagtggc
    12601 tctagttcgt ccagctcaag ttcttctaat acagcttcag gaacgaaata tacagttaaa
    12661 gccggagact ctgtttggag cgtctcaaat aaatacggaa ttacgatggc tcagctaatc
    12721 caatggaaca atatccaaaa taactttatc tacccaggac aacaactgac tgtttcaaac
    12781 agtggatcaa caaacagcac gtcaggtaat acaggttcgt caaccaatac aaataacagc
    12841 agttcagtta atactggcag cagttcgtct caaggcactt ataccgtgaa agcaggtgaa
    12901 tctgtttgga gcgtggcaaa taaaaacggc atcacgatgg atcagttaat cgaatggaat
    12961 aatatcaaaa acaacttcat ttatccagga cagcaattga tcgttaaagg tggaacttcg
    13021 gcaaatacaa atacaggttc aactacttct gcagcgaaac caaatacacc gaatacgtct
    13081 gctacgacaa gtacatcatc cactggtaat acgatgtata ccgtgaaagc gggcgagtca
    13141 gtttggagcg tagcgaacaa acatcatatc acgatggatc agttaatcga atggaataat
    13201 atcaaaaata atttcatcta tccaggacag caagtgattg tgaagaaagg ttcatctcaa
    13261 tcaacaaacc aacaatcagt tactggttcg aaaacctata ctgtgaaatc tggagaatct
    13321 gtttggagtg ttgctgacag tcatggaatt acaatggctc aactaatcga atggaacaat
    13381 attaagaaca actttattta tccaggtcaa acgttgattg tagctaaata aaatagctgt
    13441 tcgaagaaat aacgattgaa ttccacaaaa atatttgtta ctataaggct acttaaatat
    13501 gacaacactt gaaaaggaag tagtaaaaag actgaagttt cagagagcgc atggctggtg
    13561 agaatgtgca cggatgcttt tgaactcgcc tttgagttgc ccttgctgaa tacccgagta
    13621 agcaagcggc ggtgacggcc gttacaacgt tcgaggcctt ttcactaagg taaaattagg
    13681 tggtaccgcg tagattacgt cctataaaaa gagcaatctt tttataggat ttttttattt
    13741 cttttgtgaa gtttgagaaa acaaagcaaa aggaaataaa aataaacaga ttctgccggt
    13801 tttttcgtta tatttataat agaaacaagt gttttcaata gaacaggagg acagcaaatg
    13861 agttacaatc ataaagaaat tgagaaaaaa tggcaaaaat actgggcaaa aacgaatact
    13921 ttcaacaccc atgatgatcc tgaaaagcca aaattttacg cactggatat gtttccctat
    13981 ccatccggtc aagggctaca tgtagggcat cctgaaggtt atacggcaac ggatatcctt
    14041 tcacgtgtga aaagaagtca aggatacaac gtgctgcatc caatggggtg ggatgcattc
    14101 ggtctgcctg ctgaacaata tgcgctagat acaggaaacg atccagctga gtttacccaa
    14161 aaaaatatcg aaacattccg acgccaaatc aattctttag gatttagtta tgactggaat
    14221 cgcgaagtga atacaactga tccggagtat tataaatgga cacaatggat ctttacgaag
    14281 ctttatgaaa aaggtctagc ttatgaagca gaagtagcgg taaactgggt acctgaatta
    14341 ggaacagtta tttcaaatga agaagtgatc gatgggaaaa gtgaacgtgg tggctatgat
    14401 gtagtcagaa aaccaatgcg ccaatggatg ctgaaaatca ctgcttatgc tgatcgctta
    14461 ttggatgatc tagaattagt tgattggcca gaaagcatta aagaaatgca aagaaattgg
    14521 atcggtcgtt cagtaggagc aaatattgaa tttaaagtgg ctggtacgga taaatcctat
    14581 accgtattta cgacacgacc agatactcta ttcggtgcaa cttactcggt tcttgcacct
    14641 gaactagatc ttgttagaga aattacgacc cctgaacaaa aagctgctgt agaagcttat
    14701 atcgaagaga cagctaaaaa atcagatcta aagagaacgg atcttgcaaa agaaaaaaca
    14761 ggagtattta ctggtgctta tgcaatcaat ccagttaatg gcaaggaaat cccaatctgg
    14821 atcgccgatt atgtattatc ttcatacggc acaggagcca tcatggctgt tccagcacat
    14881 gatgaacgtg actttgaatt cgctcaaact ttcggactag aaattcttcc agttatcgaa
    14941 ggtggagatg ttgaaaaagc tgcttacaca gaagatggta cgcacatcaa ctctgaattt
    15001 ctagacggca tgaacaaaca agaagcaatc gataaaatga atgaatggct agaagaaaat
    15061 ggcgtaggga aaaaagaagt cagctatcgc ttacgtgact ggctattctc ccgtcaacgt
    15121 tactggggtg aaccaatccc aatcatccat tgggaggacg gaacagtcac agctgtccca
    15181 gaagaagagc taccattacg gttgcctaag acaaaaaaca tcaagccaag cggtactgga
    15241 gaatctccat tagcaaatat cgaggagtgg gtcaatgtcg ttgatcctgt aacaggtaaa
    15301 aaaggccgtc gcgaaacgaa tacaatgcca caatgggcag gaagctcatg gtattacctg
    15361 cgttatatcg accctcataa caaaaaagaa ctagcagatt atgaaaagtt gaagcgttgg
    15421 ctaccagttg acatctacat tggtggagca gaacatgcgg tacttcattt actatatgca
    15481 cgattctggc ataaattctt atacgatatc ggtgttgttc ctacaaaaga accattccaa
    15541 aaactataca atcaaggaat gatcttaggc gaaaacaacg aaaaaatgtc aaaatctcgt
    15601 ggtaatgtag tcaatcctga tgatgtggtc gaaacttacg gtgcagatac actgcgcatg
    15661 tacgaaatgt tcatggggcc attagatgct tcgattgctt ggagcgaaaa tggcttagaa
    15721 ggaagccgta aattcttgga tcgtgtatgg cgtttgatcg ttgatgagaa caataaaatg
    15781 cgtgaccgta tcactacatt gaatgatggc aagcttgata aagtgtatca tcaaacagtg
    15841 aaaaaagtga cagaagacta tgaaaatctt catttcaata cagcaatctc ccaattgatg
    15901 gtctttatca atgaagccta taaagtggac gctctgcctt atgaatatat cgaaggattt
    15961 gttcaactac tcgcaccgat tgctcctcac atgggtgaag aactatgggc aatccttgga
    16021 aatgatggtg gtatttctta cgcaccatgg ccaacatacg atgaagcagc acttgttgaa
    16081 gacgaagtag aagttgtttt ccaagtaaac ggtaaagttc gtgcgaaaag caatgttccc
    16141 cgagacttag gaaaagatga gctagaaaaa gcagcattag ccaatgaaat agtgcaagaa
    16201 tatattgaag gcaaaacagt cagaaaagtt attgcggtac caaacaagtt agtcaatatc
    16261 gttgctaatt aattggctct ttgtcaataa ggactgatga gttgtgcaaa atcaaatctg
    16321 ggccagaatg aacttcattc tgtctcagat tttttgttat ttcaaattga ttaattgatt
    16381 gatcaaaaag gttttgattt tcatgttctc aaacacgttc aatgtctttt tcttctgaat
    16441 ggtcttgtaa gagatctctg taatcatcaa atttttgtct attaatctta gtattttttg
    16501 atgtataaac tacctgaaat tgagagtaca taccaaattc ataaattttt tattagtggg
    16561 actagtgatt gtatggattt tgacgcataa tgaatgaagt atgagaaaag gagagacaaa
    16621 tggatcaaga actatttatt atcgaagtat tatcagctaa acaaaaaatt atcgagaaag
    16681 cgaaaaaaag agcgaaaaga ataatgttca agcttttaag cttagtattt ttctctggtt
    16741 tgcttttttt cggagctgta tcgttcctat atcgccatat gagtgcagta gtccgttcag
    16801 ctgaagggat gcaaaaatta aataatttgt atcacttttc gtggggagtt ggcggaataa
    16861 caattgttgt gcttattgtt tttcttctac ttatttggct ccgttaccat caattgtcgc
    16921 gtcatttcga tcaatggctt agtcaaagag cacaacagtt tttacataat aaaccaactt
    16981 ctcaagatga tgactatctt tattttgaga agacttcgac gagtcaagct tttcggatac
    17041 aaaaaagtga ttgccagtta ttgctgagat cacttgatga tttacctatc tacttaggcg
    17101 aaagcaaatc aaaattcgga atgaacacat tccatatttt tttactagaa aacgaaccca
    17161 gatgggaaaa aagccagtct tcgcctggtg gatacctgac aactaagatg agtacattag
    17221 tcactggttt tttcctcatt cttttagctg gaacaatcta ttatgggaga tacactcagc
    17281 caaaaatgtc ttctcatata aatgctgctt ctcacatgac aatgaactca actaacgata
    17341 cgcatataca aaagacaaat gaagggttgt tagctgatcc taagcaaaag gtaaatttga
    17401 caacacaaaa gataaatgat ctcaaacttg atacagagac gcactctctc tatatgacaa
    17461 cagatagtgg tgctaattgg gtatttgtgc cgattgattc gcaatggttg cgttttggcg
    17521 aatacaccct gacaacgggg ctgattcctg aaggttattg gatggacaac acatatgata
    17581 tttccgccga tttctcatgg tttatctatt cttctgggga tgatgtacgt ctgctgactt
    17641 caagagataa tggtaaaaca tggcaaacgt ctattatcac ccaatcagtt cagccgatac
    17701 gttatcgaaa agcggtattt tatggcaacg atggtctatt tatctgtaca tcagccagtg
    17761 ggatgtctgc agaaactttg tacatttaca ctactatgga tggcggaatc acttggataa
    17821 aaagtggaag tacaatgatc aatcagccag tacaaaatgt cagcttcgtt acaacttctt
    17881 taggttttgt ttcgacacgt gaaagtttgt tttatacaaa caacggagga cgctcattca
    17941 aagaatcagt tctgtcgatt ccggaaaact atcagacagg ggggatagat ctttttcaat
    18001 caccaaatga agttactcaa ataagcgcga atacgttaga gacaaaattt tatttgttga
    18061 aaaaaggacc aatcgatgca gggaaaatgt ttgcctgcct cttccgttca actgataacg
    18121 gggaaacatg gcaattcgtt cagcaactat cgcaagtaga acaaggagat taaagaagag
    18181 agattgtgag cctgacgttc agcaccgagt cacaagaaac aaattcaaaa aacagcttct
    18241 catatttgtt gaatagtgta actcgtgatt cggagttgac aagagcaaca agaagcgatt
    18301 ttgaagtata gcttctcgta ttttacaaga tcgtgtctta ttgccgatag gttgtctctt
    18361 gaacaccgtt tattcgtaaa aagggattgt gacaagtctt ttgtcactac ttaccgaacg
    18421 aaactgaagg agctatccaa aaagtatcat tagtattatt ctttgtgttt ctagtggcac
    18481 cactagtaca tttaaaaaag ctatcgatca tggactaatc atacatgatc gatagctttt
    18541 tagataatgt ttgttacgct atctgaaatt gactattata aagactggca taaagcgttg
    18601 gtgattggag tagttcagaa tgtgttcctt tttcgacgat atcgccattc ttcattacaa
    18661 ggatcatgtc tgcattttca atcgttgaca aacggtgtgc aatcacaaaa cttgtcctat
    18721 cttttgtcac agcttccatt gcttgctgaa tcaatgcttc tgttcgtgta tctacgctag
    18781 aagttgcttc atctaaaata acgacaggag gattcgctag aatgactcga gcaattgtca
    18841 ataattgttg ctgtccttga gataacgtgc cgttttcgct agaaatgatc gtatcatatc
    18901 cgtctggcag tgttcgaata aagtgatcac attgtgcgat ttcagccgct tcgataattt
    18961 gttctcttgt ggcatccatt ttcccgtagg cgatattatc agctaccgtt ccttcaaaca
    19021 gccaggtatt ttgtaaaacc atcccaaaag aacgacgtag ttctccacgt gaaagcttgg
    19081 taatatcaag accgtcgaat tcgatctttc ctttattcag ttcataaaaa cgcatcagca
    19141 gattgactaa agtcgttttt cctgcaccag ttggaccaac gatcgctacc gtttgttttg
    19201 gctttactga aaaatcgact tgattcatca atattttatc tgatgtgtaa ccaaactgta
    19261 catttttgaa agctattttt cctttcggtt gttctaaaag tcttgcatct ggaaggtctg
    19321 gaatttcatc tgtttcatct aatatctcaa atatcctatc gattgctgcc attgcggatt
    19381 ggatcgagtt gatcacataa gaagcggtag aaattggttc agaaatctga ttgatgtact
    19441 gaagataagc ttgtaagaag ccgatcgtca caccacctga tagaacaagc atagcaccga
    19501 ataccgcact tatgatgaag gcgagttgat taatgaaacg aatggccgga tagatagcac
    19561 agttcataaa ttgagcacgt ttaaaagcgt tgtattgctt tttatttact tcgtgtatag
    19621 cagcttctgc atgagcttgc tgattgaatg ttttgaccac tagattacca gctaagattt
    19681 cttccattcg attatttaaa acacccagtt catactgatt ctgttcagaa atctgtttgt
    19741 ttttgttcgc aatcttagtt gtaacaattg aactgccaac gatcagcaat aagacgagca
    19801 aagtaagctt gctttcgata taaaacagca tcagaccagc agcagctatt gttacgacag
    19861 aagtaaaaaa ttgattgata cctgtcaata acacttgaga aagttgattg agaccggtag
    19921 ttgtccgact aatgatgtca cctacttgat gattgtcaaa aaacgacatc ggtaatgttt
    19981 tgaatttttt tgtgatttct tttcgcaccc tcaatgtgat cttttcgctt aacgctgcca
    20041 ttgtccgttc ctgaataaat gaagtgatgc tgcttaagac tgaacagccg atcagcaata
    20101 agacagggaa caacaatgct tgtttgatat cactgaaaga aagctgtcca atacctgttt
    20161 ttcttatttc tgataagaga tcatcgatcc cgattcccat gatcaagggc atggctacga
    20221 ctaataggtt cccgaataaa ctgcatagaa acaatccgta aaaatattta tgttcaggtt
    20281 tgatcatttt ccaaaaacgt atcaaagcac gcgtgttcaa cgtttttttc ttttccatcg
    20341 tatcccttct ttctatattc cttgtgattt tgcaaactcc tgataagttt ggcaattaga
    20401 cagtagttct tcatgagtac cttgtccaac gatttttcct tcatctaaaa caatgatgtt
    20461 atctgcttgt tggattgtac tgaggcgttg agcaacaatc acgagagttt tctccgacat
    20521 gtgtttatga agggctgctc ttaatgctgc atctgtttta tagtctaaag cggagaaact
    20581 atcatcgaat acatagatct ctgctggttt gatcaacgct cgagcaatac atatccgctg
    20641 tttctggccc cctgaataat tagaaccgcc ttgagcaaca aagctgtcca atccgtcagg
    20701 aagggtgtcc actagttctt ctaattgagc gatcttgatc gctttagaca actcttctgt
    20761 tgaggcatat tcatttccca tctgcaagtt gcttaaaatt gtcccgctga ataagaaagc
    20821 cttctgaggg acataactga tgtgggagcg gatctgttct tgagtcattt gagtaagagg
    20881 aataccacca agggttactt ttcctatcgt agggtcacta agtctcaaga gaagtttcgc
    20941 caaactactt tttccagatc cggtcccgcc aacgatcgct gttgtttttc ctttaggtat
    21001 tgtgaaatca atatcttcta gaactggatc agagatttca gtatatttaa atgtgacatg
    21061 ttcaaaagca gctgcatttg tctgatcaaa ggttggttca gctactgttt tgatcgtctc
    21121 ttcctcaatt tcgatctctg cttgcagtat ttcttctatc cgtcgtaatg aggcaaacga
    21181 tttagggata atgaccaata ccatagctgc gatcatcaag taagcaagtg tcagcataga
    21241 gtattcgact acagcagtca cgacaccgat ttgtaatgct ccttctgcta caagatttcc
    21301 gccaaaccac aaaatagaag cataaacgat tcccatgagc aaaaaagcaa ttggagtgac
    21361 ccaagcaaat attcggttca ctttgatcat ttgattagca tagtcagcaa agctcttatt
    21421 cgttcgcttt tcttcatatg attgattatc aaaggcacgg atcatattaa tcccagtaaa
    21481 gaattctctc aatgtgatgg tgatccgatc catttttggc tggatactca aagataaagg
    21541 tgcccctcgt ttcatcagtc gataaacaat ccatacataa aaaacaacag agatcaatgt
    21601 aacccatgtt agtaatagag aataacgaaa catcatgaga atggtgaaaa cgcaaataac
    21661 cggtgctggt aagatcattt ggcaaaataa aacaatcatt tgctggacat tatcgacatc
    21721 attcgtcatt cttgtcaaaa gagatccaga actaaaacga ctgatatcct tcattgacag
    21781 ctgttggaat ttacggaaat atttctcccg cgtttggaat ccaaacctag cggaagccaa
    21841 tgctgaaaag taactagagg caactgcagc gattgttcct agaaacgcta caagcagcat
    21901 ttcaactcca atttttttta taactgaggc ttcgccactt gctatacctg tgtcgatcag
    21961 ctttgctact aattttggta cacctaatgt accggcgatt tctaaacaga tcatcacaaa
    22021 ggtaaggaga gcgagtcctt tgtttttctg taagaatgtt tttaagatag acatattatt
    22081 cctccttttt tcctgatact tttcgattct ataccttcaa gtaacttgaa ggtcaataga
    22141 aaaaagaata aactgaaaaa gaggtgagaa aaaatgaaaa aaatgtatac gattggccaa
    22201 gtatcggaac tatttgattt gccaaaatca acgatccgct attgggatga acaaggatta
    22261 atccgttcaa gtagacaaga agagaatgat tatcgattgt ttgatatcga tgatattttt
    22321 atgctttatg atatcgtttt ttatagaaaa ttagatatac cgatcaaaca gatgaagaat
    22381 ttatatggaa aaacattgac ggagttatat gagacacttg atgaaacaga aagaagaatc
    22441 cataaagaac tggtggtgat gaaacaaaaa caaaaagaga taagggagca aaaaaaacag
    22501 ttgaaattga tgatcgatac aaacgaggaa gaatttcctg tagaagagat cccttttgac
    22561 tgtatgatct caacggagtt tgaagatatt gtggaaatca aaaaatttct acctaactat
    22621 tcaagctttg ggatgatgag tatgccagct tccagttcac agacgatgta tggatttttt
    22681 atcgacccta gtgaagtaca tttatttact caagacgtga tttgggagaa aaaagatacg
    22741 gctgtttatc gtcgattctt attgaaatcc gagatgaacc atgcggaacg taataatatt
    22801 ttagaaatca gagaaagaat gtatgaaaaa ggatggaaaa caggagaagt gatcggccag
    22861 tatctattga cgaatacgga tgaaaacaat atcagaactg aatattatca tgcatggata
    22921 gaaatgaaaa aataggaaat atgtgtttaa gataactcga aaatgatggt tgttttagtt
    22981 ataaagaaaa attttctggt tcgattacgg gtttaatgtg ggggattatt agtcgtggat
    23041 agatagaatt ttagtttttt agaggaaata tgtctttaaa aatggaatgg ttgcaattct
    23101 tagcgatata tacgatgtaa ttgtcctctt aaaaaagtat ggaaaaataa ttgtattggg
    23161 tgttatgtat aattcatggt gaactgagac taatgagaag aaaaattcat attgttatga
    23221 gtgagattgt atcagatgag aatgataaat taaataggtg cataaaagca ttacacttaa
    23281 aatacattgt tgcttacatt attacaatat gttaataagg caaaaataaa tagatggatt
    23341 gaataaacat attttttaat caaatagtcc ctactgtgac tttcctcagc tatcggtgat
    23401 actaccgata gcattttttt gcttataaaa taattataag tatttagaga tttttcttct
    23461 aacattcttc aaacaagaaa aatca
//
LOCUS       DAJSAW010000039        23261 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJSAW010000039 DAJSAW010000000
VERSION     DAJSAW010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.039"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ONT64_001784"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ONT64_001784"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR2303195.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ONT64_001785"
     CDS             complement(1172..1867)
                     /locus_tag="ONT64_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCR2303196.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ONT64_001786"
     CDS             complement(1880..2596)
                     /locus_tag="ONT64_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HCR2303197.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ONT64_001787"
     CDS             2926..3534
                     /locus_tag="ONT64_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCR2303198.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ONT64_001788"
     CDS             3859..4182
                     /locus_tag="ONT64_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCR2303199.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ONT64_001789"
     CDS             4364..5734
                     /locus_tag="ONT64_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCR2303200.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ONT64_001790"
     CDS             complement(5864..7165)
                     /locus_tag="ONT64_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR2303201.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="ONT64_001791"
     CDS             complement(7519..9477)
                     /locus_tag="ONT64_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCR2303202.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ONT64_001792"
     CDS             complement(9654..9920)
                     /locus_tag="ONT64_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR2303203.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ONT64_001793"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ONT64_001793"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR2303204.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ONT64_001794"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ONT64_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR2303205.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="ONT64_001795"
     CDS             13264..13827
                     /locus_tag="ONT64_001795"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR2303206.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ONT64_001796"
     CDS             complement(14080..14601)
                     /locus_tag="ONT64_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HCR2303207.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ONT64_001797"
     CDS             complement(14604..15446)
                     /locus_tag="ONT64_001797"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCR2303208.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ONT64_001798"
     CDS             complement(15439..16164)
                     /locus_tag="ONT64_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR2303209.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ONT64_001799"
     CDS             16340..16636
                     /locus_tag="ONT64_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303210.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="ONT64_001800"
     CDS             17204..18796
                     /locus_tag="ONT64_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR2303211.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ONT64_001801"
     CDS             18809..19549
                     /locus_tag="ONT64_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR2303212.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="ONT64_001802"
     CDS             19717..21066
                     /locus_tag="ONT64_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2303213.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="ONT64_001803"
     CDS             complement(21194..21784)
                     /locus_tag="ONT64_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCR2303214.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            22020..23099
                     /locus_tag="ONT64_001804"
     CDS             22020..23099
                     /locus_tag="ONT64_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2303215.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT         6661 a         4820 c         4527 g         7253 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aaaggcggat tttataatta acttagccat ttggaaataa aaaaacgcca
    21961 cgtgaattcc atgttatatt gaagttacca caactctcaa tagacaagga tgaattcaca
    22021 tgacgcaaaa ccatgataac acagtttcac gcaagggaaa acacctaacc tactcagaac
    22081 gttgccaaat cgccatcctg aaaaaggaaa actattccaa ccgtcaggtt gcggatgtcc
    22141 tgggctgcgt gccgcagacc ataaacaacg aaatcaaacg cgggaccgtt actcagctta
    22201 accgccagaa tcagaacggg aaggtctacg actattatca tgacgtctac gacgccgatg
    22261 ccgggcaggc ggcctacgag aagaaccgct tgaactgcgg cagacggccc ttatgggccg
    22321 acacggacgc cttcattgaa tgggcggacg acatgatgct gttggaaaaa tggtccccgg
    22381 acgtggtggt gggctttgcc agaaaacacg aattgttcga cccggccatt atccctggga
    22441 ccaccaccct ctatcagtgg atcgaccgtg gcatcatgaa aacgaagaat atggacctgc
    22501 tagaaaaact gtcccgcaaa ccgaaatcgt cccgtcatgg caaccgcccg aataaacggg
    22561 tgctagggaa atccattgaa gagcgcccgg aggcggttgg tacccggaaa accttcggcc
    22621 attgggaagt cgacacggtc gtaggaaaca agaccaagtc ggatgccgtg ctgctgacgt
    22681 tggtggaacg gcagacacgc ttcgaggtca ttctgaagct ggccgggaag gatgccgaat
    22741 cggtcaacca agcggtccga cagctgagag aacgggctgg tgcgtacttc ggccagctgt
    22801 tcaagacggt tacctcggac aacggctcgg agttcgcggg actgcacgat gccttgcagg
    22861 atgtggcaaa catctacttc agccacccct atgcttcctg ggagcgcggg acgagcgaaa
    22921 accaacacaa gctgattcgc cgtttcatcc ccaagggcag cccaatcggc aacgtgagcg
    22981 aaaaccaaat cttgcggatt cagcgttgga tgaacgatta cccgcgaaag attttgggct
    23041 atgcaacgcc gcatgacacg ttcgtccagg catttaggca ggaacggttg acggcttgac
    23101 acgtgggccc ttgacaacga gcctccctcg gcatggcttg tgtctggaaa gctggccggg
    23161 gccagccagt cgggagacta ccatgcctca gctcattgtc aagggcccac tcgattagaa
    23221 aaattagacg gttggtggta acgagcaacc gggtcaccat g
//
LOCUS       DAJSAW010000040        23077 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJSAW010000040 DAJSAW010000000
VERSION     DAJSAW010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.040"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            102..2543
                     /locus_tag="ONT64_001805"
     CDS             102..2543
                     /locus_tag="ONT64_001805"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCR2303216.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="ONT64_001806"
     CDS             complement(2873..3577)
                     /locus_tag="ONT64_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303217.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3764..4009)
                     /locus_tag="ONT64_001807"
     CDS             complement(3764..4009)
                     /locus_tag="ONT64_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303218.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4026..4877)
                     /locus_tag="ONT64_001808"
     CDS             complement(4026..4877)
                     /locus_tag="ONT64_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR2303219.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5043..6047
                     /locus_tag="ONT64_001809"
     CDS             5043..6047
                     /locus_tag="ONT64_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HCR2303220.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6288..6644)
                     /locus_tag="ONT64_001810"
     CDS             complement(6288..6644)
                     /locus_tag="ONT64_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR2303221.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7012..8199
                     /gene="tuf"
                     /locus_tag="ONT64_001811"
     CDS             7012..8199
                     /gene="tuf"
                     /locus_tag="ONT64_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR2303222.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8387..10978)
                     /gene="mprF"
                     /locus_tag="ONT64_001812"
     CDS             complement(8387..10978)
                     /gene="mprF"
                     /locus_tag="ONT64_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCR2303223.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11004..11765)
                     /locus_tag="ONT64_001813"
     CDS             complement(11004..11765)
                     /locus_tag="ONT64_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2303224.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12043..12564
                     /locus_tag="ONT64_001814"
     CDS             12043..12564
                     /locus_tag="ONT64_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HCR2303225.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12623..13483
                     /locus_tag="ONT64_001815"
     CDS             12623..13483
                     /locus_tag="ONT64_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCR2303226.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13668..15983
                     /locus_tag="ONT64_001816"
     CDS             13668..15983
                     /locus_tag="ONT64_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR2303227.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16165..18006
                     /locus_tag="ONT64_001817"
     CDS             16165..18006
                     /locus_tag="ONT64_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR2303228.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18030..19139
                     /gene="rpoD"
                     /locus_tag="ONT64_001818"
     CDS             18030..19139
                     /gene="rpoD"
                     /locus_tag="ONT64_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR2303229.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            19359..21458
                     /locus_tag="ONT64_001819"
     CDS             19359..21458
                     /locus_tag="ONT64_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCR2303230.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            21654..22517
                     /locus_tag="ONT64_001820"
     CDS             21654..22517
                     /locus_tag="ONT64_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR2303231.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            22622..>23077
                     /locus_tag="ONT64_001821"
     CDS             22622..>23077
                     /locus_tag="ONT64_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR2303232.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         7771 a         4038 c         4810 g         6458 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa
     3721 atggaacgga ttatgtatat atacataatc cgttccattc tttctatttt ttcggtatca
     3781 aggcaaaaac aggtagttca ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca
     3841 ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat ttgatcatgt gtccagtttc
     3901 ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag
     3961 tctgggtaac ttcttccgag agagaattca agcgttcgat tggatccata aaatcacctt
     4021 tcgacttaaa attgggtagt atctgggtct tttgctttgc cgactacacc atctaactga
     4081 tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa ataattcagt attttctttg
     4141 attctgcttg gtgtcactga cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc
     4201 aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca
     4261 tcgaagattt taccagtgcc taatggacta tatgcttcga ggatcatatc atgttttttt
     4321 gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc
     4381 ggcataattt ttgctgtttt tgctagtgta tctagatggt ggggtaagaa attgctcaca
     4441 ccgattgctt tgattttacc tgcttcatac agttcttcaa atgctcgcca tgtatctgca
     4501 tttgcttgtt cccaattatc acgaaaagca acagggtttg gccagtggat caaaaacaga
     4561 tctaaataat ctgtttggag cttcttcaat gattcttcaa aagaagacat cacaagttca
     4621 tagctgtggt ttgcattcca taattttgtt gtcaagaata tctcttctct aggaattcca
     4681 ctttccttaa tcgcttggcc gacgctttct tcattcttgt atccttgggc agtatcgata
     4741 tggcgataac ctgcagccag cgcttcttta acagaggaaa cagcgacatc cccatcaggt
     4801 gtttgccatg tgccgaatcc tactaccggg atttcgtaac catttgatag acgataagtg
     4861 cttgttaatg aattcatact ttcagctcct tttttattcg ttcaactata agtgtagcgc
     4921 atttttcgaa aaaaagaatc cattttgttt ctactcttga aatttttgtt taaaagaaaa
     4981 tgattttgat tctggtattt ttgatttttt ttccgtaaac taagacagaa gggaatgaga
     5041 aaatggatat ctatttaaaa aaagcaattc tgcatattat tgacagagaa actggttctc
     5101 cagtcttttc acaaaaagag ctggatctga caaaagaata tattcgtgat tttcttcaaa
     5161 agaagatcca aaaaatctct tctgctcaaa caaaaactgg acaactagca gaagaaagca
     5221 tgatcgttga atcacttaaa atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg
     5281 ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc acctagtgca gatgtatttg
     5341 ttgtcttgta tgaattagat acagtgatgt acttatcttt attgaaagtc aattatcgtg
     5401 aggcttacac tcactttgtt gaagcagacg aagcagggat cgacaaccgc ttgattttga
     5461 atcgagcaat tctaggggcg aaaacacaaa aagcagatga agcattcgct gtgaatttga
     5521 ctgattggac ctacgaatta attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt
     5581 atttttctgg aagagtgatc gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag
     5641 tgatccaaaa agtagccaag catcttggaa aaaaatttga gacagaagaa tttgatatca
     5701 tggctgattt gaaagaagct gtctatgata cgatagaaga aaaaggtcgg ttggatcatg
     5761 agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc aaaaatggct tttaaagaag
     5821 aagtacaaga gcaaggattt gtcccagaag cacctcctgt aaaagaagta caagaaattt
     5881 ctgagaaaaa atttggaaaa caaaaattca agctatcaaa cgggatcgaa ctgatcgtac
     5941 cagtagatgt atatcgcaac ccagatttga ttgaatttgt gaataatccg gatggtacga
     6001 tatccgtgat gatcaaaaat gtggatgaag tattgaatcg tttataaaaa tgactagaat
     6061 attaggtgtt caagtaccag tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa
     6121 agacgaatat aacaaaaaaa gagcgtgaga caaaagtaaa gaacactttt gtctcacgct
     6181 ctaaacacgt ataaacggcg gaagcagaag caactccttc ttatttcttg gagttaaacg
     6241 cttctgtccc agcctctttt tatctaataa aattttaaga ccatttacta attatcgatt
     6301 ttttctgccg catttttagg cacttcagaa gcctttattt cttcccagct taatacacct
     6361 ttacgaggat tgactttgag tttaagatac gcattcatct ttaatggatg ttctctttct
     6421 tcgcgaagcg tcacctgttt tttttcacca tctttattat aagccgcttg cttgtaggta
     6481 taagtcgtga atttctctcc gttatctgct tgggtagctg acatttcgcc attcgtcgtg
     6541 atttttgtat aataagcttc gccgccgtaa aaataatgat aagctgcaaa acttccgccg
     6601 agaaatatgc ctaaaacgac aagtacgcca attaattttt tcatgtactt cacctctttc
     6661 cttattatta acgaaataga agatttcgtc tattgaaagt tgttggcaac agctgaattt
     6721 taaacttttc ctcattttgt tcttattgct tcaaaagaaa gttaaggact atcttgaagt
     6781 ttttctcaaa aaacacattt ttttatttat aaaaggtact tttttcgcgt ataagacaga
     6841 cgagtgctct tagttgactt tgaccaatat tgacgtataa taagcttagt tcgaatctta
     6901 caaaaagtaa gtcggacgta ttctttttga tttagtcaaa aaaagactat aataaaaagg
     6961 tgagagctga aatttttcag tgatctatat tatttaggag gtttgtaaat tatggcaaaa
     7021 gaacattatg acagaagtaa accacacgtt aacattggta cgattggaca cgttgaccat
     7081 gggaaaacta ctttaactgc tgcgatcact acagtattag gcaaaaaagg cctagcaaat
     7141 cctcaagact atgctagtat tgatgcagct ccagaagaac gtgaacgtgg gattacgatc
     7201 aataccgcac acgtagaata tgaaacagaa aaacgccact acgcgcatat tgatgcacca
     7261 ggacacgcgg actatgtgaa aaacatgatt acaggggcag cacaaatgga tggtgcaatc
     7321 ttagttgttt ctgcaactga cggtccgatg cctcaaacac gtgaacacat tttattgtca
     7381 cgccaagttg gtgtaaaata cctgattgtt ttcttgaaca aagttgattt agtcgatgat
     7441 gaagaattga tcgatttggt agaaatggaa gttcgcgagt tattgagcga atatggtttc
     7501 ccaggcgatg acactcctgt gatcaaaggt tccgcattaa aagcattgca aggcgatcca
     7561 gatgctgaag ctgctattat ggaattgatg gatacagtag atgaatatat cccaacacca
     7621 gaacgtgata cagataaacc attactattg ccagtggaag acgtcttctc aattacaggt
     7681 cgaggaactg ttgcctcagg tcgtattgat cgtggtgctg ttcgtgtcgg tgatgaggta
     7741 gagatcgtag ggatcaaacc tgaaacacaa aaagcagttg taacaggtgt agaaatgttc
     7801 cgtaaaacgt tagattacgg ggaagctggg gataacgtag gcgtgttgtt acgggggatc
     7861 caacgtgacg atatcgaacg tggacaagta cttgctaaac caggttccat tactccacat
     7921 acaaaattca aagcagaagt gtacgtgttg acaaaagaag aaggtggacg tcatactcca
     7981 ttcttcaaca actatcgtcc acagttctac ttccgcacaa ctgatgttac aggaacaatc
     8041 acattgccag aagatacaga aatggtcatg cctggtgaca acgtgacgat tgatgttgac
     8101 ttgatccatc caatcgctgt tgaaaacgga acgacattct caattcgtga aggcggacgg
     8161 acagtaggtt caggtatcgt tacagagatc gaagcataag aaacgcgact cgaatatttt
     8221 ggtaatgaaa aatgtttaaa ataaaagagc ggtccaaaca ggatttctgt ttggaccgct
     8281 ctttgttctt caattgtata agaagagatt gtagaagcat gaactttcgt tttacttcag
     8341 tctcttttat tcatcatgag agtgttcatc tatcggtatg cagtttttaa tactttcttc
     8401 gtatccattt tttcaaacca cttattctac cttgtgtctg ctcaactggc gcattatcaa
     8461 taatcagcaa agcaatcata acatatgcaa tccaactatc tctagaatat agagtataac
     8521 gtgagaccca gtttgtagcg tacttgcttt tatattctct taatccatgg aaagaataaa
     8581 aatgagaacc aaactcgtaa accaagtatg caatcctttc ctgtaaaaaa ctctttctgg
     8641 aagttccgac atttgaaaga ggggccatcc ctaaattgaa ccattggata tgttcttctt
     8701 tcatataggc aaacaaatgc aagaacaaga aatccatact gccagaaggt gcttttttag
     8761 aatcatatcg catcagatca atcgttccta ctttattgtt cgtgtaagta ggcatgatcg
     8821 ttgcaaaagc gacaatttct tcttgggtat ttttgacaac tgcaataggc gctctttgta
     8881 gataatcttc agagaaaaag cctaaagaaa accctttttc ctttcgtgaa cccaaccaat
     8941 tatcagagat atttttgaaa gtactcattt gctcagtaga aaatggtggt tgtaagacat
     9001 caaatgtaaa tccttctcgt tcgatccgat tcaagacagc acgagtgccc ttcatttttt
     9061 tccctgaagt tgtaaaacta ttcaggtcta cataagcttc ttcccccatc ttaatgaaat
     9121 cgtagccgaa ttcatgcaaa atcataacaa tctcttcgct tgtttcgtag aaaactggta
     9181 aatagcatag tcgatctgtt tcttcgataa aagcttcgat tgcttctgga aaatcttctt
     9241 tcttaccaga aggatcaccc attacaatac atttattatt gaagcaagcg aattggagaa
     9301 gaacagttgg ttcaccgtct ttttcatagg caaacatccg cttatctttt aagaagatca
     9361 attgactatc ggagtttcct ccataagttg taaggatctt cagggccttt tcttcattaa
     9421 aggcttcgcc aatctgtttc ttttctcctt gaaggaaatg aacaaataga acgatcatga
     9481 aactaacagc gatgatggcc aacaatcccg aaaaccaaat cttttcagaa ggaaataaga
     9541 aaaaggaaat aaaatggtga cgccgatgag ggaaatccgg cagattgtat accccaataa
     9601 cgatgtaaag caaggacagt gcaccaataa tgattccatc gatcgtacgc cactcccatg
     9661 aatagatcaa ttgttcccga aataattcgt ttttagaagc aagaatgatc agaagcagaa
     9721 tagacaagaa aaccaccggt gtgaaactaa aatcacttaa taaaacataa agtaaagcta
     9781 gaacaatcaa gaagattgta ggcaaatatg ctcgtttcac acgtgctgcg atccctctgc
     9841 ccataatgat taaaagaaat cccaatagta tactaggaaa ttggataata aaatgaaatt
     9901 tcaatgggtt caaactatga agccagcgaa actctgtaaa agcttgcggg atagtggcag
     9961 aaagtactag catgattcca gaaaagtata ataagacaac cacgatttta tgcgcaattt
    10021 ctgttgctaa ttgtttaggg ataccagaat aacgctgatc aaaagtggaa ccgatatttt
    10081 tgaagaaaaa gacgattcca atcaaaaacg gaatgatgta gtagaacagg cgatagagca
    10141 agatccacac aacgactatt tctcttggca caccaatagc ggacaacccg ataatcatca
    10201 ttacatcgaa actcccaatt tctcctggaa tcatagacac gattcctatc actgaagcag
    10261 caatgaataa tggaattgtt tgccacagat caatccggat ttccattaaa taaccaacag
    10321 aaaggaaact aaacaggaca ccggtccatt ccaacaatga aactagcaat agctgtcctt
    10381 gagttttaag tgaggtacca ccaaggaatc ctttcttttt gaaagtagta aataaaaaga
    10441 cgacaggaaa atatagaccg ccgccgatca accatatcca atattgcttc aaaaaaggag
    10501 caactggcgt gaagactaca agtaaaaaag aaagcagact gtaaatagaa agtccagcca
    10561 tcaaaaacaa gaaaactttc gataatgctt ggatcacgtt tctgctctct ttcttttgtc
    10621 catatagttc agagcgaagc cccacactaa caaacccgcc gaaaccggca atattgttga
    10681 tcgtattgat cagccagctt gtctcaaaca ggtaacgagg attctgcttt tgttcaagca
    10741 tccgattcaa aataatatca taaccaacca taggtaacac agagataaga ccaatcaata
    10801 gcattagtcc gcttttccat acaggtatag tggcaaacgt ttctcctaat tgctggatag
    10861 atagagtttt accgatggac atcaattcac cgacaataat gacaatgaca gaaatcaaaa
    10921 agattgtttt gaacagtcct aggtggtttt tcagccattg tatggtattt tttaacaaat
    10981 aaatcacctt ctattcctaa tagttatgtt tcagaaagaa attgaagaac ttctcgattg
    11041 aagcgttcag gatcttttct agctaattca tgtccttgtt ctttcacaag cacaaaggaa
    11101 gcttttggta tagttttagc tatatacaaa gaatgtttta gcttgattac atcttttttt
    11161 cccacgatga tcaaagtggg ggagttgatt ttttttaaat cattttctgt aagcccgatg
    11221 tcatgcagca aaagtttgat aactaaaaga tttttgcgaa gacttgggcg aaataaagag
    11281 agtaaccata cccatgcata atgaatatta ctgatgactc tggcagaaaa acgaacgcct
    11341 ttaacaagcg tgtttccaga atttaaaatc aatcgatgaa cttttttagg gaagctgctg
    11401 gcaaatacca gtgctagatt tgctccatca ctaaatccaa gaaaatctgc ctgatcgatt
    11461 ttttccagca acatgatcgt attcagatcc tcggccatta actgaaaatt caacatagat
    11521 gcttcgtttg tcgaacgacc atgtcctcgg ctatctacta gatacacggt atagtagcgt
    11581 tcgagtacag ggacctgttc tgaaaaaaag cggccgctgc cgtcattgcc atgcagtaaa
    11641 aagagaggga agccttgacc gctcttttca tagtaaattt cgctcccatc tggcattttt
    11701 gcatatttct tttctttttt cacataatca cctactgtgt aaagtataac gattatatca
    11761 gtcattgggc aattgttttt actgaaaaac aacgatcatt ttcgtaagat gtcaaaaaat
    11821 acaggaaaat tcattttttc ttaaggcgta cacagaaatc aaggaagaaa tagccaagaa
    11881 gataaagaaa tagtaagata aaaaaagatg tcaaaataac taggtgatga aatgaagcga
    11941 aaacgattat ctattatccg tattcttttt tacatgatca ttctgacggt tattggtgcg
    12001 gtgttgtttt tatgaggaag aacttatcgt tcggtaaaac gagtggaaag ctatcaaata
    12061 gaaataacaa cagctgcaaa aaattatcat atggaaaact acgagaattt gatcaaggcg
    12121 ataatattga ccgaatcaaa aggaaaaggg acagatctca tgcagagctc ggaaagtctg
    12181 catggagaag ctgagatcat tgataatcca gctgacagta ttgatcaagg tgtcaaatat
    12241 ttggcggaaa tgatagtaga agcaaagaaa caaggatgcg atttatggac agctgtccaa
    12301 gcatataatt ttgggaaaga ttatattgcc tatgtaaaaa atcatggcgg agaaaacaca
    12361 gtagaattag cagaagcgta ttctaaagat gtgctgagcc cacttttagg aaatgaggaa
    12421 gggaaacaat accgatattg gcggatccag tccttgcttt acaatggcgg ctatctctat
    12481 cataatggag gtaacatgtt ttatcctgat attgtcaaaa tgaatgaaaa aataatagag
    12541 acctatgaaa aagtctttaa gtaaaatagc aggaaatgtt caatcatgaa taaaattaaa
    12601 tgaaagaagg agtgcgcaaa tagtggaaaa aaggcaaaaa atggaaaatc aagaagtggg
    12661 gtacacaacc aaagactgga tgttacggtt tgtgaaggga atgtttatcg gttcaggatt
    12721 tatcttgcca ggagtcagcg gtggtgcatt agcagctgtc tttggcatgt atgagcggct
    12781 gatttctttt ttagcccacc ttacaaaaaa tttcaaagaa aatgttttgt tttttctgcc
    12841 tgttggtcta ggcggagtga caggcatctt tctactttct tttttagtca gttttttatt
    12901 aggtgcgtat gaaacaacga tattatggtt ttttgtcgga tgtatcgctg gaactgttcc
    12961 tgcgttatgg aaagaagcag ggaaaaaagg aagagatact tctgatgtga tcatcatggt
    13021 tgttactttt gttctggctt atttgttttt attatacggc gctcaattat ttgacgggca
    13081 agtcgatcag aattttggaa catggctgat tgccggcgga ttgattggtt tgggtatgat
    13141 cgtcccagga ttgagccctt ctaatttttt ggtgtatatg ggattgtata aagcgatggc
    13201 tgacggattt aaagaagtac gacttgaagt gattgtgccg atcggaattg gtgggattct
    13261 ttgtgttctt ggattgtcaa aaatcatgga ttacattttc tcaaaggcat atagcaagct
    13321 attccatttc attttaggtg tagtttttgc ttcaacgatt atgatcattc ctaccgatta
    13381 cgcgggattg ggagttgctg gggtactagc ttgtcctgtt ctttttatag ccggtacagc
    13441 actaggttgg tggatgagcc gtttggagga acaatataag taaagataga ttgataataa
    13501 aaacagcaca cgggtgattc aaacaaaaag gttggcatct tttttgtaaa atcattcgtg
    13561 tgtttttcta taaattttat aaaaacaagt tgtttttaat cttggaacac gctagactta
    13621 aacacgtgat tatttttgaa agttggcgga aaatggagaa aaagaaaatg aatggactta
    13681 cacagcaaga ggtagttcag aaaatggctt ctggccagca aaatgactac caggaagatg
    13741 catcaaaaag tacaaaacaa atattcagcg ataatatatt gacgttattc aattttctga
    13801 actttggtat tgggatttgt ctgttatttg taggtgcgta ttccaacatg gcgtatttag
    13861 caattattgt cgtcaatatc gttattggaa tttatcaaga aatacatgca agaaatcttg
    13921 tacggaaatt atcgattgtt tcccaatcag aagtactagt gatccgggac ggtaaagaac
    13981 aatcgatttc tactaaagag ctggtattag gagatttggt aaaaatcgga gcaggagaac
    14041 agataccagc cgatttacag gttatatctg gacgggcaga agcaaatgaa gctcttttga
    14101 caggcgaatc tgatttgatt gtcaaagaaa ctggagctga attgctttct ggaagctttt
    14161 tgacaagcgg ttctgtcatg gctgaagtta tccgagtagg tgcagataat tatgctgtcc
    14221 gcttgactag cgaggcaaag actcacaaag ctattcgttc tgaacttgta gattcgattc
    14281 gcaaagtatc caaattcact agtttcgtga ttattccttt ggggatcata ctatttttgg
    14341 aagcactgtt catccgcgat gcttctatca aaatttctgt tattgcatct gcagcagctt
    14401 tgttaggcat gcttccaaag ggtttggtat tgctaataag tattgcatta acgacagggg
    14461 taaccaaact ggcaaaaaaa cgaatccttg ttcaagacat gtattcaatt gaaacgctgg
    14521 cccatgtcga tacattatgc ttggataaaa cagggaccat tactgaaggg aaaatgagta
    14581 tccagcgggt agaagcacta catccgctgt acgagaaagc catcccagaa attatgggga
    14641 cgtatctggc agaaagcagt gataacaaca tcactatgcg ggctatgaga gattattttg
    14701 agctttctaa tcggtatgat gtgacaaaca aagtcgcatt ttcttctgat cgcaaatggg
    14761 gcgcgatgga atttccagaa ctgggagtcg tgtacgtagg agcgcctgaa cgtttggtgg
    14821 aagaagatcg gcttccggac agtctaaaaa cagcccaagc gaatggctat cgcgtactta
    14881 tgttggggat cgctccaaat cagcaattga acgaagaaaa accagcattt gttgagccgg
    14941 ttgctctgtt tgaaatcgat gatccaatcc gaaaaaacgc tcaagaaaca ctagcttatc
    15001 ttcatgagga aggtgtggat ctgaaagtga tttctggaga caatcctttt actgtctcca
    15061 atatcgctag acgtgcagga ttaccgaatt atgatgcgta tgtcgatctt tctcaaatta
    15121 ctgaagaaat ggaagtgcga gaagctgctc atcgctatac ggtttttgga cgagtgagtc
    15181 cgcagcaaaa gaaattgctg gtaaatgaat tgaaagaaag cggcagaacg gttgcaatga
    15241 caggcgacgg tgtgaatgat gttttggcgt taagagaagc agattgcagc attgctatgg
    15301 cagaaggtga tggagcaaca cgtcagattg cgaatctggt attgttggac tctgatttta
    15361 ctacattgcc agaagtcctg tttgaaggac gccgagtggt caacaatgtg acgaaagtct
    15421 caggcatctt ttttatcaaa acgatttatt catttatttt atcgattata tgtgccgtta
    15481 cagcaattgg atttccgttt atccctatcc aaatcacgct gattgattta gcgattgaag
    15541 ggtatccttc cttcttttta tcttttgaac aagataaaag aaaaatcact taccgttatt
    15601 tgcctacagc acttattaat gctttaccaa atgccttgct tgtagtagtg aatatcattg
    15661 cggtgtatct acttggaaaa acattcgcat ggggaaatgc tgaaactact actttgatgt
    15721 attacttgct gattgggatc agttgcatgg cagttgtaaa agcttgttat cccttcaacc
    15781 cattgcgcat cttcctagca gtaacgacga tcgtagggat atatgtagct gcaatgctgt
    15841 tccatggaat tttggaagta aacttagggt tgggagagag cgtaggatac ttctttgtat
    15901 tcatgctcgt aaatattgtt cttcgagtat tgtactggaa ggctgatatt caaaatcagt
    15961 tactgaaaag agtagcaaat tgatgcttaa tgagatagaa atgtgttata cttgaaaaag
    16021 caatgaaata tgatacaatc aaacatgtat aattgtgtcc tttaaaatcg cactgatttc
    16081 agttagtgcg attttttacc ggttattctc ctgaaatatc ttctgttaaa cccaaaagat
    16141 atcaatagag cagggggtga aaaaatggct caacggattc ctcaagaggt tatcgaaaca
    16201 gtacgcagtc aaacgaatat cgttgaggtg attggacaat atgtccagtt gaaaaagtct
    16261 ggtaaaaatt acttggggct gtgtcctttt catgaagaac gtacaccgtc tttttctgtt
    16321 gcagaagaca agcagatttt tcattgcttt ggttgtggta aaggcggaaa cgtgtttact
    16381 tttcttcaag aactagaagg tctaagtttt ccagaagcag tggtaaaagt ggcagacttc
    16441 gaacagatac cgttagaaga tcaatggcgt cagcgtacta tagctgtcca aaatcctgaa
    16501 tctgctacgg ggaaactgat tgctgctcat gaaaaagcag cggaaatcta tcatcacatg
    16561 ctattgcata caaaagctgg acagccagct ttagaatatt tgcaaagtcg tgggttgtct
    16621 ttagaattaa ttgaagaatt caacataggt tttgcaccta atgagcgttc ttttcttcag
    16681 caagtattca agaatgaatc aatttcaaat gaattgttag aacgctctgg attgtttgtc
    16741 acgcatgatg atggccgtat gtctgatcgt ttttatcaaa gagtgatgtt tccacttcgt
    16801 aattctcaag gccagacgat cggtttttca ggacgctggc ttgcacctga aaaagggcag
    16861 gaaaaagatc agcccaaata cttgaatagt ccagaaacag aactgttcaa taaaagacaa
    16921 gttttattta atcttgataa agcaagaagc gatatccgga aaaacggaga aattttgttg
    16981 ttcgaagggt ttatggatgt gattgctgca tggcaggctg gtatcaaaac tggattagct
    17041 tctatgggga caagtctaac agctcaacag atccaacaaa tggaaaagct gacgaaagaa
    17101 ttggtctttt gctatgacgg agacaacgca ggttttgaag caaccaatcg agggattgaa
    17161 ttgcttcggc aaaacagtcg attagcactt tctgtcgtag tcgttccaga aaagcttgat
    17221 cctgatgaat atctccgtaa gtatggagaa gcatctttcc aagaattagt ggctcatggg
    17281 cgcgaaacag tctttacttt taaatcacgt tatttaaaac aagggaaaaa tttagataat
    17341 gaaaaagaca agatcgaata cctcgaagaa ttactagttg aacttagccg agttgtttca
    17401 ccaatcgaac aagatatgta tctatctcaa ctgtcaacag agtttcagat cggcagagag
    17461 acgatacaaa aacaactgcg cgatcttcga aggacaaatc agcaaaatcg tcaagaacct
    17521 caggaaagtc aacacactca ggttacgaga caagaaactg cacggaaaaa gagacctttg
    17581 acgcaagtag aaaaggcaga gcagattcta ttatatcgtg catttaaaga agtttctgtc
    17641 cgtaatattt taaaagaacg agaatttcag tttattcatg atgcatatgc agaagtttat
    17701 tttttattcg atgcatatgt ctcacaacat tatgaattca accttgcaga gtttttagac
    17761 tttttgcaag atgaaaattt aaggcggttg gtggtggaaa tcgaatattt accggtggca
    17821 gaagaaagtt cgccacgaga aatccaagac ttgctagatg tgatcagcaa atctggctta
    17881 gctgatgaaa taacattaaa aaaacaaatg cagcaagaag ccagacgaac tggaaacaaa
    17941 cagcttgagc ttgaattgac gattgaaatc atcaatttaa cgaaacagtt aaaacaagcc
    18001 aaatagctac tcaagggggg ccttctttta tggcaaatgg aacagataaa aaaacgtatg
    18061 aagcagcagt agcagctttc atcaaagaaa acaaacccaa acgacaagtt gtatatgatg
    18121 atttatccaa tcagcttgct acaccattca ctttgaatgc agatgaaatg gacaaattga
    18181 tccaaaagat tgaagacgca ggtatcagtg tggtggatga aaatggtgat ccatcaattc
    18241 acagcttgaa gagtgctgag aaaaaagctg aacaagcaaa aacagaagat ctttctgcgc
    18301 caaccggtgt aaaaatcaat gacccggttc gtatgtatct gaaagaaatt ggccgtgtct
    18361 cccttttaac tgcggaagaa gaagtagcgt tagcattgaa aatcgaagaa ggcgaccaag
    18421 aagcgaaaca gcgtctggca gaagcaaacc ttcgtttagt agtaagtatc gctaaacgtt
    18481 atgtaggacg aggcatgcag ttccttgact tgatccaaga aggaaacatg ggactgatga
    18541 aggcagttga aaaatttgac taccgcaaag gatttaagtt ttcaacctat gctacatggt
    18601 ggattcgtca agccatcact cgagcaatcg ctgaccaagc acgtacgatc cgtattccag
    18661 ttcatatggt tgaaacaatt aataaattga tacgtatcca acgtcaattg ctgcaagacc
    18721 ttggaagaga acctacgcca gaagaaatcg gtgcggaaat ggatcttcca acagaaaaag
    18781 tccgagaaat cttgaaaatc gcgcaagaac ctgtttccct tgaaacgcca atcggtgaag
    18841 aagatgactc tcacttaggt gatttcatag aagaccagga agcaacaagt ccagcagaac
    18901 acgcagctta tgagttacta aaagaacaat tggaagatgt actagataca ttgactgatc
    18961 gggaagagaa tgtccttcgc ttacgttttg gtttagatga tggccggacg agaacattgg
    19021 aagaagtagg gaaagtattt ggtgttactc gggaacgtat tcgtcagatc gaggctaaag
    19081 cattaagaaa attgcgccat ccttctcgtt caaaacaatt aaaagatttc ttagaataag
    19141 cgtggactat tgaccagaag agaaatcagt aaaactgttt ggtttatttt aaaagaaaaa
    19201 aagcttgaga ctacgcagtc tcaagctttt ttttctatag gaaggaaatt cattcattat
    19261 ttttaagtaa atcctaaaaa taagctattg gcgtgacgta tagaatatga tagattaaat
    19321 ggtagcgtaa acctagtaaa ggagccgatc gctaagtgat gaaaaaatgt ccaaattgcg
    19381 gatcgaaaaa aatggagcag aatactatct gtccagactg cggtcatcaa ataactgcag
    19441 aagatcagca aataaaagaa tcaattgaag aaacaaatac agatgcagct atgcaggata
    19501 aatcaactga agatgcagtc tttcctgata cagaattaaa cgatccaatc gaatggtcgg
    19561 aattgaaaga tttacctcta gaatctgtaa tggaattatt cgaaaaagca gaaccagaag
    19621 acgaagaacc acctgctcac gatcaagtaa ctaatataga gaaaaatgaa cagaagaaag
    19681 caactgttca tgagacttcg gaagaatcag gaaagaatca agaagaaacg cttgaatcaa
    19741 aacaaaaaag aagagtagct gaactgaaag aaatcgtcga taatgaggaa gaaaattcga
    19801 tactatccgc ttatatcaaa gcacatcgtg aagatacaaa agaagagcac gcgaaagagc
    19861 tgctcaaaat gatcagtgaa aaaattgcac aggaaaacaa agtagagcca gaaaaggaac
    19921 aagaaactga aaaagactct gatagagaaa acgccacagg taaagaaaca gtaaaaataa
    19981 aagacgtaaa aacggatatc acagaaaaaa ctgaagtatt tactgaaaaa caattagaaa
    20041 aggaaaaaac gaataaactg ccggaagcag agaaaaaaga aatactcgaa aacacagaca
    20101 aaaagtcaac agagcccgat gaaataaata gtgaatccga tgctgaaaat caatctgtaa
    20161 gcaaagaggg tactgatctt gaagcaaaaa tagaaaaaga agagaaccag cctaagctgt
    20221 ttgaagcaga agcgaaatca ataacaatcg tgccagatac tgaaaaaaca catcaagaat
    20281 caaacttaga aaaaacagat ccagataaag cgcaaaagaa aaaatcaaaa aaagtacctt
    20341 atctcgtttt agcagcagta cttctactcg gtggaggaag ctgggcttat tatgaccatc
    20401 atcaaaaagt agaagccgaa gtagcagcag aagcaaaaag acagcaacaa atggaagaac
    20461 ttcatgagaa acttgctgcc ttttatacag atgatcacca tcaatttatt cgtgcagata
    20521 tgattcatca agatttatca aaactaaaaa atgatttatc cagtcttaag aatgaaaaag
    20581 gatattcaga gttagaaaca acttaccaag atatccaatc taagatccga gatatccaaa
    20641 aagtgaatga gtggttcata gcacctgcca tcgtcgatga tcatctagca gatgacatca
    20701 agttgaaagc agataaagag atccaagcaa tcgaaacgga taacacagat tttggaaaat
    20761 tgattcaaca agcacaaaca gaagctaaaa atcaatatgc tgaattgcaa acagctaaag
    20821 aaaaaacagc tatagtcttt aaagacggaa aagtagtgga ttcagcaaca cgcgaccaat
    20881 acaagacggc aaaagaagca gtagaaaaag tgaaaaatac agatctatct gctcagttga
    20941 atgaacaatt gaaacaagta gataagatgc tcactgataa agagaaaaaa gaagaagagg
    21001 aaaagaaaaa agcagaagaa gcgaaaaaag cagctgaaga acaagcaaaa caagccgctc
    21061 aagcagcagc tgcagctcaa gccgctcaac aagctgcggt cgcacaagca gcacagcaag
    21121 aagcggcatc ctcgacccct tcctcttcaa actcagcgaa tcagccaatc aaatctgcca
    21181 gagcaagtga tattgccgat gcatctaatc cagcatggac gtgggcacca ggtgtccaaa
    21241 gcaaagtgat tgctacatgt atccaacgtg gatatatcgt tgaaggaggt tacagattag
    21301 aaaaagccag aatcgaaaat ggggaaggct attataatct atatgccact tcaacaaaat
    21361 ctgcattgat gaaaggcatt ggagaaagtg ctttgccatt ttacatcgta accatcaact
    21421 gtaaaactgg atggttcgga ggaaatggga gcaattaatt ttaggcaaat gaaagaggct
    21481 gtgagcctgc cgtttaacac cgtttattcg aaaaagggga gtatgacaca actgttgtca
    21541 tactcctttt ttcacctttc caaaccaagg ctcagctatt caagtagata acattatgtt
    21601 atgatagcaa agaaatgtat taagaaaacc tttcactata gagggagaaa aaaatgaacg
    21661 atttattagc aactatattt acaggtatta ttattgatga aaatgaaaaa aactactttg
    21721 tccaaaaaaa cggacaaacc tttggcttaa gcaaatcaga aggtgaacac aaaatcggtg
    21781 aagcagttga aggattcggt taccaaaatc aaaaacatga aaatcggatc actacacaaa
    21841 ttcctaaaag ccgtatcggt cattacgctt ttggaatcgt tactgctaca cgaaaagatt
    21901 taggtgcgtt tgtcgatatc ggtcttccag ataaagactt tgtcgtatct cttgatgaat
    21961 tgccgacaat gacagagcta tggcctaaaa aaggggatcg cttgatgatt tcccttagag
    22021 ttgatgcaaa agaccgtatt tggggcagtt tagcagatga aaaaatattc aaatctttaa
    22081 gcaaatatgg aacagaagaa atgaaaaaca aagatgtgat aggaacagca taccgcttga
    22141 aattaacagg tacgtacgtt ttaacggaag atttctatct tgggtttatc catccttctg
    22201 aacgatataa tgaacctcgt ctaggcgagc agataaaagg tcgagtgatt ggtgtccgac
    22261 cagacggtgt actgaatctt tcattgaagc caagagcata tgaagctata tcagatgatg
    22321 cacaaatgat tttaaccatt ttagaacgtt ctgcagatca gcaaatcaac tttacagata
    22381 aatcggatcc agaagaaatc atgaaagctt ttggtatcag caaaggagca tttaagcgcg
    22441 cattaggtaa tcttttgaaa caaggattga ttacacaaga aaacggtgtg acaaaactag
    22501 tcaaaaaatc taattgagaa tcccttgcat gtttttctta actagcttat aataatttta
    22561 attaacatag tgttatatct ttttttattt gtaattatta taaattgttg gggttgagta
    22621 aatggtcatg aatgctattt cggcgatgaa aaaaacaaaa cagcagctac atgaagcggg
    22681 gtttaagctg acaccgcaaa gagaagctac tctgcttgtg cttttggaaa atgagaaaga
    22741 gcatttatct gcagaagaaa tcttcttttt agtgaaaaaa aagagtccgg aaattggact
    22801 tgctactgtt tatcgaaccc ttgaaatctt gacagaattg aaaattgtcg ataaagtaag
    22861 ttttaatgat ggattagccc gttatgattt gagaaaagaa ggcgccaaac atttccatca
    22921 tcatttacta tgcttgaact gcggcacgat cgaggaagta gaagaggatc ttttacttga
    22981 agtggagcaa gtagtagaag aacgttatca ttttttagta aaagatcatc ggctaacgtt
    23041 ccatggaatc tgtcaggaat gctatgaaaa agaaaaa
//
LOCUS       DAJSAW010000041        23065 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJSAW010000041 DAJSAW010000000
VERSION     DAJSAW010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.041"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(109..564)
                     /locus_tag="ONT64_001822"
     CDS             complement(109..564)
                     /locus_tag="ONT64_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303233.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="ONT64_001823"
     CDS             complement(718..1926)
                     /locus_tag="ONT64_001823"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCR2303234.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="ONT64_001824"
     CDS             complement(2038..2370)
                     /locus_tag="ONT64_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCR2303235.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="ONT64_001825"
     CDS             2518..3393
                     /locus_tag="ONT64_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2303236.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSGELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="ONT64_001826"
     CDS             complement(3475..4056)
                     /locus_tag="ONT64_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCR2303237.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="ONT64_001827"
     CDS             complement(4062..5321)
                     /locus_tag="ONT64_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR2303238.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="ONT64_001828"
     CDS             complement(5323..6369)
                     /locus_tag="ONT64_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303239.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="ONT64_001829"
     CDS             complement(6604..6879)
                     /locus_tag="ONT64_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCR2303240.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /gene="der"
                     /locus_tag="ONT64_001830"
     CDS             complement(7122..8432)
                     /gene="der"
                     /locus_tag="ONT64_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR2303241.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="ONT64_001831"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="ONT64_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR2303242.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /locus_tag="ONT64_001832"
     CDS             complement(9978..10658)
                     /locus_tag="ONT64_001832"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR2303243.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="ONT64_001833"
     CDS             complement(10707..11342)
                     /locus_tag="ONT64_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2303244.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="ONT64_001834"
     CDS             complement(11408..12811)
                     /locus_tag="ONT64_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR2303245.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="ONT64_001835"
     CDS             complement(12798..13829)
                     /locus_tag="ONT64_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2303246.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="ONT64_001836"
     CDS             13860..14081
                     /locus_tag="ONT64_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCR2303247.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="ONT64_001837"
     CDS             complement(14246..14983)
                     /locus_tag="ONT64_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HCR2303248.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="ONT64_001838"
     CDS             complement(15136..16476)
                     /locus_tag="ONT64_001838"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HCR2303249.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="ONT64_001839"
     CDS             complement(16713..18236)
                     /locus_tag="ONT64_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCR2303250.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18614..19225)
                     /locus_tag="ONT64_001840"
     CDS             complement(18614..19225)
                     /locus_tag="ONT64_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR2303251.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19348..19473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19555..20271)
                     /locus_tag="ONT64_001841"
     CDS             complement(19555..20271)
                     /locus_tag="ONT64_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCR2303252.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="ONT64_001842"
     CDS             complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="ONT64_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCR2303253.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20830..21627)
                     /locus_tag="ONT64_001843"
     CDS             complement(20830..21627)
                     /locus_tag="ONT64_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCR2303254.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21597..21968)
                     /locus_tag="ONT64_001844"
     CDS             complement(21597..21968)
                     /locus_tag="ONT64_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR2303255.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="ONT64_001845"
     CDS             complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="ONT64_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR2303256.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6467 a         4926 c         3911 g         7761 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccc ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctggtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    18361 tttccctatt ccctcctctt ttttcagttt tttaaagctt tcttcttttt cattgtagcg
    18421 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    18481 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    18541 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg
    18601 atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac
    18661 ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt
    18721 tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca
    18781 tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg
    18841 cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc
    18901 ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg
    18961 ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca
    19021 gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca
    19081 tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga
    19141 tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca
    19201 ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa
    19261 ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag
    19321 ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt
    19381 atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg
    19441 gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca
    19501 ttatacatat ctattcaaaa aacgcaacat ctaacaatta taaaaagtaa gtgttcattt
    19561 tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg
    19621 taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc
    19681 aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac
    19741 gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt
    19801 tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc
    19861 ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc
    19921 aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac
    19981 aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc
    20041 atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc
    20101 ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa
    20161 ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt
    20221 tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat
    20281 tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt
    20341 tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag
    20401 tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca
    20461 atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc
    20521 tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg
    20581 gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct
    20641 tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag
    20701 ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga
    20761 tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg
    20821 gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat
    20881 tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa
    20941 agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt
    21001 acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt
    21061 tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat
    21121 tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc
    21181 agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc
    21241 tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt
    21301 gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtgc cgaattcttg
    21361 gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc
    21421 tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt
    21481 gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag
    21541 cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac
    21601 tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca
    21661 cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc
    21721 tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc
    21781 cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt
    21841 tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc
    21901 agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt
    21961 gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc
    22021 gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct
    22081 aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta
    22141 tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc
    22201 agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc
    22261 atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat
    22321 tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt
    22381 accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc
    22441 atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc
    22501 gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc
    22561 tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc
    22621 cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct
    22681 tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc
    22741 catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag
    22801 ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg
    22861 atttgctctt tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt
    22921 ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact
    22981 ggtttctttt ttcggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc
    23041 ccattttcat tttaggtatt aaaaa
//
LOCUS       DAJSAW010000042        22028 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJSAW010000042 DAJSAW010000000
VERSION     DAJSAW010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.042"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(61..1371)
                     /locus_tag="ONT64_001846"
     CDS             complement(61..1371)
                     /locus_tag="ONT64_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCR2303257.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="ONT64_001847"
     CDS             complement(1383..2801)
                     /locus_tag="ONT64_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2303258.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="ONT64_001848"
     CDS             complement(3041..4609)
                     /locus_tag="ONT64_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCR2303259.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="ONT64_001849"
     CDS             complement(4613..4894)
                     /locus_tag="ONT64_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCR2303260.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="ONT64_001850"
     CDS             complement(4891..5814)
                     /locus_tag="ONT64_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HCR2303261.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="ONT64_001851"
     CDS             complement(5811..6689)
                     /locus_tag="ONT64_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HCR2303262.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="ONT64_001852"
     CDS             complement(6676..8013)
                     /locus_tag="ONT64_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCR2303263.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="ONT64_001853"
     CDS             complement(8016..9119)
                     /locus_tag="ONT64_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR2303264.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="ONT64_001854"
     CDS             complement(9193..10701)
                     /locus_tag="ONT64_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR2303265.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="ONT64_001855"
     CDS             complement(10667..11359)
                     /locus_tag="ONT64_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR2303266.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="ONT64_001856"
     CDS             complement(11381..12355)
                     /locus_tag="ONT64_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2303267.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="ONT64_001857"
     CDS             12655..13527
                     /locus_tag="ONT64_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2303268.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="ONT64_001858"
     CDS             complement(14109..16307)
                     /locus_tag="ONT64_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2303269.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="ONT64_001859"
     CDS             16539..17531
                     /locus_tag="ONT64_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2303270.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="ONT64_001860"
     CDS             17591..19012
                     /locus_tag="ONT64_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR2303271.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="ONT64_001861"
     CDS             19005..20504
                     /locus_tag="ONT64_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR2303272.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="ONT64_001862"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="ONT64_001862"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR2303273.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6670 a         4494 c         3823 g         7041 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaaat
//
LOCUS       DAJSAW010000043        21988 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJSAW010000043 DAJSAW010000000
VERSION     DAJSAW010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.043"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..395
                     /locus_tag="ONT64_001863"
     CDS             <1..395
                     /locus_tag="ONT64_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303274.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="ONT64_001864"
     CDS             398..1147
                     /locus_tag="ONT64_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2303275.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="ONT64_001865"
     CDS             1159..1428
                     /locus_tag="ONT64_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303276.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="ONT64_001866"
     CDS             1433..1597
                     /locus_tag="ONT64_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303277.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="ONT64_001867"
     CDS             1590..1892
                     /locus_tag="ONT64_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303278.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="ONT64_001868"
     CDS             1889..2050
                     /locus_tag="ONT64_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR2303279.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="ONT64_001869"
     CDS             2047..2352
                     /locus_tag="ONT64_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303280.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="ONT64_001870"
     CDS             2352..2708
                     /locus_tag="ONT64_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HCR2303281.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2695..2913
                     /locus_tag="ONT64_001871"
     CDS             2695..2913
                     /locus_tag="ONT64_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303282.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="ONT64_001872"
     CDS             2910..3329
                     /locus_tag="ONT64_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HCR2303283.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="ONT64_001873"
     CDS             3326..3883
                     /locus_tag="ONT64_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCR2303284.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="ONT64_001874"
     CDS             3880..4176
                     /locus_tag="ONT64_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303285.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="ONT64_001875"
     CDS             4253..4666
                     /locus_tag="ONT64_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCR2303286.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="ONT64_001876"
     CDS             5012..5386
                     /locus_tag="ONT64_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303287.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="ONT64_001877"
     CDS             5423..5590
                     /locus_tag="ONT64_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303288.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="ONT64_001878"
     CDS             5616..5960
                     /locus_tag="ONT64_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR2303289.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="ONT64_001879"
     CDS             5965..6246
                     /locus_tag="ONT64_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303290.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="ONT64_001880"
     CDS             6349..6663
                     /locus_tag="ONT64_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR2303291.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="ONT64_001881"
     CDS             6641..8335
                     /locus_tag="ONT64_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR2303292.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="ONT64_001882"
     CDS             8355..9533
                     /locus_tag="ONT64_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR2303293.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="ONT64_001883"
     CDS             9496..10182
                     /locus_tag="ONT64_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCR2303294.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="ONT64_001884"
     CDS             10182..11342
                     /locus_tag="ONT64_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR2303295.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="ONT64_001885"
     CDS             11352..12227
                     /locus_tag="ONT64_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303296.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="ONT64_001886"
     CDS             12224..12535
                     /locus_tag="ONT64_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303297.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="ONT64_001887"
     CDS             12525..12878
                     /locus_tag="ONT64_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR2303298.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="ONT64_001888"
     CDS             12868..13269
                     /locus_tag="ONT64_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303299.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="ONT64_001889"
     CDS             13262..13666
                     /locus_tag="ONT64_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303300.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="ONT64_001890"
     CDS             13678..14286
                     /locus_tag="ONT64_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR2303301.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="ONT64_001891"
     CDS             14306..14668
                     /locus_tag="ONT64_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303302.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="ONT64_001892"
     CDS             14671..14853
                     /locus_tag="ONT64_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303303.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="ONT64_001893"
     CDS             14870..18289
                     /locus_tag="ONT64_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR2303304.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="ONT64_001894"
     CDS             18340..19077
                     /locus_tag="ONT64_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR2303305.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="ONT64_001895"
     CDS             19087..21378
                     /locus_tag="ONT64_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR2303306.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="ONT64_001896"
     CDS             21402..>21988
                     /locus_tag="ONT64_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303307.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3246 c         4705 g         6104 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatctg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DAJSAW010000044        21535 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJSAW010000044 DAJSAW010000000
VERSION     DAJSAW010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.044"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            216..917
                     /locus_tag="ONT64_001897"
     CDS             216..917
                     /locus_tag="ONT64_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR2303308.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1526..2542)
                     /gene="tsaD"
                     /locus_tag="ONT64_001898"
     CDS             complement(1526..2542)
                     /gene="tsaD"
                     /locus_tag="ONT64_001898"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR2303309.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2539..3003)
                     /gene="rimI"
                     /locus_tag="ONT64_001899"
     CDS             complement(2539..3003)
                     /gene="rimI"
                     /locus_tag="ONT64_001899"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR2303310.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(3010..3552)
                     /gene="rimI"
                     /locus_tag="ONT64_001900"
     CDS             complement(3010..3552)
                     /gene="rimI"
                     /locus_tag="ONT64_001900"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR2303311.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3536..4258)
                     /gene="tsaB"
                     /locus_tag="ONT64_001901"
     CDS             complement(3536..4258)
                     /gene="tsaB"
                     /locus_tag="ONT64_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR2303312.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4449..5429)
                     /locus_tag="ONT64_001902"
     CDS             complement(4449..5429)
                     /locus_tag="ONT64_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2303313.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5453..6937)
                     /gene="galT"
                     /locus_tag="ONT64_001903"
     CDS             complement(5453..6937)
                     /gene="galT"
                     /locus_tag="ONT64_001903"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HCR2303314.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6949..7938)
                     /gene="galE"
                     /locus_tag="ONT64_001904"
     CDS             complement(6949..7938)
                     /gene="galE"
                     /locus_tag="ONT64_001904"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR2303315.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8186..8353
                     /locus_tag="ONT64_001905"
     CDS             8186..8353
                     /locus_tag="ONT64_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HCR2303316.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8415..10226)
                     /locus_tag="ONT64_001906"
     CDS             complement(8415..10226)
                     /locus_tag="ONT64_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCR2303317.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10223..10588)
                     /locus_tag="ONT64_001907"
     CDS             complement(10223..10588)
                     /locus_tag="ONT64_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HCR2303318.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10751..11146)
                     /locus_tag="ONT64_001908"
     CDS             complement(10751..11146)
                     /locus_tag="ONT64_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR2303319.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11164..12126)
                     /locus_tag="ONT64_001909"
     CDS             complement(11164..12126)
                     /locus_tag="ONT64_001909"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCR2303320.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12126..12338)
                     /locus_tag="ONT64_001910"
     CDS             complement(12126..12338)
                     /locus_tag="ONT64_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCR2303321.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12359..13057)
                     /locus_tag="ONT64_001911"
     CDS             complement(12359..13057)
                     /locus_tag="ONT64_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR2303322.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(13077..13619)
                     /locus_tag="ONT64_001912"
     CDS             complement(13077..13619)
                     /locus_tag="ONT64_001912"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR2303323.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13751..14755)
                     /locus_tag="ONT64_001913"
     CDS             complement(13751..14755)
                     /locus_tag="ONT64_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR2303324.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14752..15741)
                     /locus_tag="ONT64_001914"
     CDS             complement(14752..15741)
                     /locus_tag="ONT64_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR2303325.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15738..16544)
                     /locus_tag="ONT64_001915"
     CDS             complement(15738..16544)
                     /locus_tag="ONT64_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2303326.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16710..17666
                     /locus_tag="ONT64_001916"
     CDS             16710..17666
                     /locus_tag="ONT64_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2303327.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17743..18261)
                     /locus_tag="ONT64_001917"
     CDS             complement(17743..18261)
                     /locus_tag="ONT64_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR2303328.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18349..18498)
                     /gene="rpmG"
                     /locus_tag="ONT64_001918"
     CDS             complement(18349..18498)
                     /gene="rpmG"
                     /locus_tag="ONT64_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR2303329.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18726..19172
                     /locus_tag="ONT64_001919"
     CDS             18726..19172
                     /locus_tag="ONT64_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCR2303330.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19367..21262)
                     /gene="asnB"
                     /locus_tag="ONT64_001920"
     CDS             complement(19367..21262)
                     /gene="asnB"
                     /locus_tag="ONT64_001920"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HCR2303331.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6481 a         4563 c         3707 g         6784 t
ORIGIN      
        1 tgttagcgtt tactatatgt attattgttg ttttttatac acaaatcatt cgcttcaaaa
       61 caaattattc aataccattg tatatttttt acataataac aaattatatt tgtaaagtat
      121 taaaacttgt ttctaatgtt ggtatgttaa aactttacaa attctttaaa aaaagtgaca
      181 ataaatatag tgaacaataa ggagaggact ttttaatgcg tgttaataaa atcgattttc
      241 acttttcaaa actcagatat caacctgatt ttgaacattt ctcggatgaa gagttccaac
      301 agatcaagga acatactgtt cttcgttctt ataaaaaagg acagatcctt ttcgacgatg
      361 gtgatcccag acaacggttg tatatcttga ccaaaggact cgttcgttta gaacgttatg
      421 atgaaagtgc aacttattac tactatgact atgtgaatac aaataacctt tttcctatgt
      481 caggaatgtt tggagaagag acctacactt atacagctca agcgatgact gatattgaaa
      541 cattctacct tcctgtttcc tactatgaaa aattagcacg aaaaaatccc gaacagttga
      601 tctatatgat acaaaaaatt tctaaagtag ttgaaataca tgaattgcgt atccaaatcg
      661 gattgacctc tagcgccttt gaccgggtaa aacaaaattt attcattttg aaagaagaat
      721 tgggcgtacc aacaaaagat ggtcatatca ccattcctta tccgatcacc ttaaaagaat
      781 tagcagtgaa tagcggcacg acacgtgaaa ctgctggaca agtaatcaaa caactacgtg
      841 attccgggct gttagattat gacaaaaagc gttttacttt ctgtaagaca gctactactc
      901 cagctatgag agaataacgc gaacaaaaat aagcagtgac cctgataaat atcagggtca
      961 ctgcttattt tcctccctaa aacttctgaa agatccggca gttttagatc actggacaag
     1021 actcaaagat tttctatgtg tgtttcagtt atgtaatatt cactgtttgc aaaaaatact
     1081 gttagattaa ctagatgaag cgtcttgtcc tcatccgatc tagattattc gatattcctc
     1141 gacaataaac gattactcag tgagggcaat cagaggaacg gataatgata aataagctgt
     1201 aaaaaatagc aaaataacaa taatgattaa aagtatgtaa aaaatagggc tttgacaaaa
     1261 acgtgtcaca gccctatttc gattaaacgg tgtgtacctg tcaactcttc ggtcacaagt
     1321 cataatgttc ataaacatga ggagctgttt tttgaaattg ttccttgtga atcagagtta
     1381 aacgacaggc ttacgaactc ttgattaaac ggcgttcaaa agcttgcata cgcggtaata
     1441 agaacagcca ggtcaaaaac atgagaagcc gtttttcctg ggctgttctt attacttgat
     1501 gcagggcagc tttttcacaa cctctttatt cagataaaac caagcttgga tcagcattca
     1561 actgatagct agcaaaattt cttttttgca tttccacatg tgctgctgct ccaatcattg
     1621 cagcattatc tccacaaagt cgaagagggg gaatcactag ttctacctca gggagtttag
     1681 ctgccaaagc atcctgcaat ccttcacgca acccttgatt ggccgctaca cctccagcta
     1741 taatcagttg cttgactgga taattttgac aagctcgaag tgttttagta atcaatacat
     1801 cgatcacact tgcttgaaag ctagctgcta aatcattttt gtctagatct tctccacgct
     1861 gttgtgcatt atgcaccaaa ttgataaatg cactttttaa tccgctaaaa ctgaaatcat
     1921 aattatcttc atggatcatc gctcgcggaa aatggtaatt atcttttccc tgatgagcta
     1981 gctgatcgat ttcttttccg cttgggtaag aaagacctaa tactcttcca actttgtcat
     2041 aagcttctcc tgctgcgtca tctcttgtct ccccaatgat ttcatatgat ccatcttctt
     2101 gcatatagac taattctgta tgtcctccgc taacaagaag tgccatcaat ggaaactgga
     2161 atggtttaac cagacgggct gcatagatat gccctgccat atggtttaca ggaataagcg
     2221 gcaattggtg tgcccaagca aaagccttag ctgcagaaat accaatcagc aacgagccga
     2281 caagtcctgg tccataagtg acagcgactg ccgataaatc ttctgccgac acaccagctt
     2341 ctaccagtgc atcttccaag cataaggtga tttgttctac gtgatggcgg ctagctactt
     2401 caggtactac gccaccaaaa cgtttatgac tgtttatttg agaagcaacg atgttagata
     2461 agatctctgt tccatttcta acgaccgcta cactcgtttc atcacaactt gattcaatcg
     2521 ccagaatcaa ttcttcattc atttcatcgt ctccgttttt aactttctgc tcataatgat
     2581 cgcatcttcc gtcggattgc gataatatga tttccgttta cctaatatac gaaacttttc
     2641 tgattcataa agttttcttg ctgcttcatt agatagacgt acttctaaaa aaacatgcag
     2701 cgaatctttt atttgttccg taatcagtaa ttgtaacaaa tgccgtgcat gcccttttcc
     2761 ttgttctgaa actgcaacag caatatttgt gatctcgact tcatctatta ctttcgagta
     2821 tccaataaag ccacagatct ggttatcttc agctaaaagc agataatcag agtgaggctg
     2881 ttggatgtct gccaaaaact gcattttcgt ccaaggtgaa ccatgtggat aagcattttc
     2941 gctaatcatc catacgcatt ctgccaagta cgcttcatca aaatcttttt ttctgcaaat
     3001 caactaatct cactcatttt ctaaaagttt tatcatatcc aacgcatctt cttttgtttc
     3061 agtgtaatat ctttttttga tcgttgttgc ttgaaacccc aatttccgat aaagtctttg
     3121 tgcatcttga ttactcatac gcacttctaa tgacatcgtc tcacagcgat tcattatagc
     3181 aaagttctcg atttcgtcaa taagcagcga accgattcgt ttacgctgat atgccggtcg
     3241 aacggcaata ttcgtgatat ggcaatctaa accgaacgct cttgaaccga taaaaccaat
     3301 cattcttcca ttatcaaaga tacctagata tagatgagga agcggtgact tcaattctga
     3361 tataaaggca ctttttgtcc atggcaattc gccattatag acttctcgtt cgacatccag
     3421 cagctctttg atatcttctg tagtcaattc tcttagtacg taacgacgat cgatttgttc
     3481 gactgttttt tctgtataaa tcattttatc ttgaaaaaag cttctggcat acgctctaaa
     3541 ttttttcaac ataactttcg tctccaggtg tgtgcgtctc caaccatttt tcttctgctt
     3601 ctactcgttt taaatagcga ggaagaaaag cttgtttctc tgcaactggt tcaactaaag
     3661 cacctaattg agccacagtt acaccacttg gaatatccca tgctggaact gtattaatca
     3721 tcgcactagg caatatttcc tttatttcag agaagaattt gtgacaatca ctcccgacga
     3781 aataaatagc ttcattcagc ttttgtagtt gtgtgaacaa atcagaagca gaaatatgta
     3841 catcttcaag tacagtttct aatctatttt ctttccagcg gtatgcccca gcgtatacat
     3901 tttttcttcg agcatcaaac atgggcacaa taatattatc cacaccgatg caattagccg
     3961 cgatggtttt cagactagat actcctacta gctctttatc taatgtataa gcaatcgttt
     4021 ttgctgtcgt tacacctata cgaagccctg tataagaacc gggaccatca gaaaccgcga
     4081 ttcggtcaat gtcttttgga cttatttgaa gatctgcaaa caattggtcg attgcaggca
     4141 tcagtgttac actgtggttg cgtttgatat ttgtttgtat ttgtccaaga atttgttcgt
     4201 cttctaccac accgatcgcc aaagtttggt ttgcagtatc tattcctaat gtaatcatat
     4261 atttgatcct ttctgctatg acattctcgc cttccattgt agcatacttt tttattctga
     4321 ggatgaaaat accaaaggca aaactagtac aataatacat gattatctgc tggcgggcaa
     4381 attttgcccc ttcctgaaac acaaaaaagc tgcggtatca ctccattttc ccacagcttt
     4441 tccatctttt atttcgttga ttctcgaata atcaactctg tttccacaat aatctttctt
     4501 gaaaccggtg ctttggaatt acttaattga agaatcgtct ccactgctag ctctcccatc
     4561 cattcggtat aaacttttac agtagaaagc gagggcgtta catatttcgc tacactgaca
     4621 tcattgaagc ccatcacaga aatatcttct ggtaccacta aacctttttc ttgaatcgct
     4681 ttcagtgcgc caatcgctaa tgcatcatta gctgcaaaaa gcgcatcagg aacatgcgga
     4741 ttcgttttta aatattgctt cactgcttga tatcctgact cgacagaaaa atctgcttcg
     4801 ataattggac cttcgtcgta gcctttttct gccaaaactt gttgaaatgc cataattctg
     4861 ggatctttta gccgctgatg gtttttcttc gtatattctg ttccagcaag catagcgata
     4921 tgttgcgctt tatgggagac aaagtgttca atgacttttt gaacacttga cttaaagtcg
     4981 acaactagag agtcgattcc ttgagaagtg ccgtcagaat ctaccaaaag caaggcattt
     5041 tgggatcctt tcatttctaa taactgagct tcgtcgaatt ttcctagtgc gatcgttcca
     5101 tctgccggaa gatcactaat ggaatcccac gtttctttca ataaagtaac gtttgcttct
     5161 tctgcctttt tttcgattcc taggcgaata gataaataat aaagatcttc aagctcttcc
     5221 gtatcgtcgt accactggat caggcgaagc gtttgcttag tatttgctgc atttttacga
     5281 tgtttcgtat aatttaagcg ttctgctgct tcaaaaattt tttgctttgt ggctaagcca
     5341 acggaaagat caggatcata gttgagaact cttgaaacag tagcaggaga aactcctgcc
     5401 aactgcgcaa tgtctttaat tgttgccatt tgtctctcct ccttttttac gtttataaaa
     5461 tcgcagtaaa tcgttcaaac gctgcctgtc ctttttcatc tcgcttgaat actccagcgt
     5521 cttccagtac acgtgcaaaa acctgaccta cagatttttg tacgacatcg gtgaccgtat
     5581 ctttcgtcaa atcaggatgt tcttttttta attgttctgc ccacggacga tgatattcag
     5641 ccatctggtt ttcttctcct aataagaaat gctctacttc tactagttct ggtttcaaac
     5701 gaggtggtaa aacagcaagc cccattactt cgatcaaccc aatgttctcc tttttgatat
     5761 gttggacatc tttatgtgga tgaaaaatcc catcaggatg ttcttctgaa acattgttgt
     5821 ctcgaaggac aaggtccaac tcaaagtaat tgtcccttct tcgtgcaatt ggcgtaatcg
     5881 tatggtgtgg tgtaccatct gctgaaaaag ctctgataga tactgtttca tccgaatatg
     5941 ctttccattt ttccaatact gagttagctg cttcaataag caagtttttc tctgttgatt
     6001 gcaaacggat cacagacatc ggccatttca cgactcctgc gatgacttgc ggataatctt
     6061 ctagtttgat aagtttttct atcggtgctt ttgccatagg aaattcatga cgtcctgcct
     6121 gataatgatc atgagaaaga atcgatcctc caacaattgg taagtcagca ttcgaaccaa
     6181 caaagtaatg aggcaagact tcagtaatcc gaagcagtct agcaaatgtt tcctttgtga
     6241 ttttcatcgg gcggtgttgt tctgataaaa tgatcgaatg ttcattgtaa taagcataag
     6301 gggaatattg gaatccccag ctttcaccgt ctagattcat tcgaataatc cggtgattgg
     6361 tgcgggctgg atagttcaag cgacctctat atccttcgtt ttccatgcaa agcatgcagg
     6421 taggatagtc tacttttacc gcctcgcgtt ctgctgcgat ttgttttgga tctttttctg
     6481 gcttagataa attgattgtg atttccagtt caccatactc cgactctgcc ggaaatttga
     6541 tgttttttgc gatggctctt gttttgatgt agtcattttt cttacttaaa ttaaagaaat
     6601 actcagttgc ttcctctggg cttttcgaat agtgctgtgc aaagaatgca ttgacgactg
     6661 aaggtggcgg tgtgagaaaa tccattaatt gcgcttcaaa aatctcttgt tctgctggca
     6721 attctgaaag aacaccattt tctcttgcct tagcgacaag ctgatccaat aagtctacag
     6781 attcttcctt aactggtctt acttcaatgc tttctagagc atcttctcct accatcccta
     6841 aaagacgatt ctgtaaatat aagcggtcca tttccatcca tccgccagct tcgatcgcaa
     6901 gtgtggcaaa atcacaaatc gtttgactga ttgacatatc tcattcccct attcttcata
     6961 accatttgga tggctgacat gccaatccca tgctgttttg ataatcgctt tgatatctgt
     7021 gtattcaggt ttccagccca gaatctctct cgctttttca cttgaagcga ctaatcggct
     7081 aggatctcca gctcttcttg gtgctatttc tgctggaatt tcttttcctg tcacttctct
     7141 agctgcttct agcatttcct tgactgagta tcctttgctg ctgcctaaat tgaagaagtt
     7201 gctttcattt ccttctttca agtattccaa agctaaaata tgtgcagcaa tcaaatcttc
     7261 tacttgaaca taatctctga tacatgttcc atctggtgta tcataatcgt ctccataaac
     7321 agccaaggct tttctttgac caagagctac ttgtaaaatg attgggacaa gatgcgtttc
     7381 tggtgtatga tcttctccga tagatgcatc tgcttttgct ccggctacat tgaagtagcg
     7441 caaagcgaca tagcgcatgc catatgcttg gtcacaccac ttcatcattt tttccatcat
     7501 caatttactt tcaccatacg gatttttagg attagctggt gtatcttctg tgattggaga
     7561 ctctttcggc tctccataag ttgctgcagt agaagagaat acgattttat tcacgttgaa
     7621 ctcgtgcatg acttcaagta agatttgcat accgtatacg ttgttgttga aatacatcaa
     7681 tggtttttcg acagattctc ctactaaaga gcttgcagca aaatggatta ctccttcaat
     7741 cggttccttt tcaaatacgg aacgtagaaa ttctttatcc cggatgtctc cttcataaaa
     7801 atgagcatct ggatgtactg cttgtttgtg acctgtgagc aaattatcta ccacagctac
     7861 ttgatacccc ttttgaacta gctggtctac agcgtgcgaa ccgatataac cggctccgcc
     7921 tagtactaaa attgtcataa aaaacactcc tttgattagt aaaattgtgt ttgttgattc
     7981 tatataattt aaaaaacgaa gtttactttt atggtgaaaa caaacaaagt aaaatttcgt
     8041 aaattcatat ggtaaattta taataaaaca tttagtaaac attttcaagg gttttttgca
     8101 aaggtttaca aacaaacgta aaactagcct ttttttctct tttgttccat aatagatata
     8161 acaaataata aatgaggtgg attcaatgaa aaaatttgta tctggaatat tagtaggaag
     8221 tctggcaaca gccgctgccg ttgctggttt ggttgcttct gtgaaaaaaa cagtgatcga
     8281 tccaattgac gaaaaagaag caatgatcga agaaaaccgt aaaaaagcaa tgcgtaaacg
     8341 cgtttctcgt taataattat ttaaaaatac gcatgaagac tttctaaaaa acagataagt
     8401 catcatgcgt atttttattt gtcagtcaat ataagttgtg ctattttatc tgcctgtctc
     8461 agattccatg gatctgtata aggtctagct actgctgcag ttagccaatc cacaccttca
     8521 acaggtattc catagcaata aaagtcagac tggatttccc ctgaagcatc aatcacgcga
     8581 ttcgtacctc gctcaacatc tatagcttct gaatgataac ttcctgtatt atctgaaaat
     8641 gaaaaagggc gaacaagttt gttttccttc aaatttttta tgacaggatt acttgaacga
     8701 gaaaagcttg tttgtggcaa tcgagcttca atcaaatagc gactgctgat ttctttatct
     8761 tgtagagaaa taacatagcg gttgtgcttt tgctgaatat caaatggcgg ttcgatcaaa
     8821 tgaaaaatac ccgcatctat cagtgctgct aattctttcg tccgaatgat tggcggaccg
     8881 atcgttaaaa agctatttag atgagtaaac catccccata gtatttcttt gtattcctta
     8941 gcagaaaaaa cgtgccattc aatagcctgc cggattggat cacgccagtc ttttaatgca
     9001 tcaaatgttg aagcaaatgc tcctgtgcaa ttgccttttt ccgcttcttt tatctgccct
     9061 tcaataaata ttcggctagc ttctgaaaag ggctcattct gataggcaag aggagtttct
     9121 aaatacgacc aatcccatct ataagggatt agtgctggat gctttccgag acactcctct
     9181 gaagagaggg agagaaaatc atgctcaaaa tttttctttg gaatcggcaa gtgatattct
     9241 tcgatgattt tcttgtaata aaacaactca acgtcttttt tcagatagtc aaaaaatgtt
     9301 tcgccagtca aaaatcccga ctgatgccat tgctccaggt tttcttttgt tagtaaccgt
     9361 ggccatgcca ttgctccgcc ttgcttttga tttcttcctc gagggtaata agggagcccc
     9421 cttcttgatc cactgtatac aatcggttcc ttgcctgatg gatgatagac cagttttccc
     9481 ttttcgttct cgaagcgccc tcctcttgat actgtaaaca atccgacata atcaaaaaaa
     9541 cttaagccta gacctcttaa aaagacattt tctttaggcg gtatcttcga aagatctaca
     9601 tctgcaggat tagaaggaag ctgatacagt aaaacatttt tctttgcgta gtctacccac
     9661 actctttctt cttctgtctc atcattttcc cagtgaccgc ttgctaaaac aagctgatcg
     9721 atagaatagc tttgctgatc tgtctgtaaa agataaccat cttttctttt gcttactttt
     9781 tttactaaag aataaacaaa ctgaatagaa gctggaaatt cttcttgcaa ctttgagaaa
     9841 aaccagcgtt gatagagtcc gtataaacat cgagtagatt gctgatcttt ttccagacga
     9901 ttgatttctt ctataattag ttgcttattt ttggttaccg tctgttcgat ataatctgta
     9961 gcaggtcctt tactccattg aaacaagttg ggtccctgtg ttaccaatcc tccactggac
    10021 aatgtttcat ctgtaaaaag cgtcacttgt tgagaaacag aattcattaa taactccggt
    10081 ggctgattca accgccaaac tcgacctcca ggaccattgg gatcgaaaag aaaaagttca
    10141 agagaatgtc tgtcctgatt attatggaga atcctttctg ctacacttaa tcctctaggt
    10201 ccggcaccaa tgatccctat tttcatttgc atgcctccat ttcgataaat tttttcaata
    10261 aaacagcatg gttctgatgt tcattttttg caccataaac aaaagtaatg actggatgtt
    10321 ctttcataag ttgactgagt ttaaaaatag cttgcttctt ttcttcttcg aaaatcaatt
    10381 cttgctggta tttctttgca aatgaagcaa atcgttctgg tacatggtca aaagttttgc
    10441 gtgcttcatt actaggtgca atttctttca accacagatc taactgcgct ttttcttttg
    10501 aaattcctcg tggccagaat ctatccacga gtattctata gccatcctct ttttcagcat
    10561 caaggtaaac acgttttaat ttgatcatag ttgtttcctt ctttctacct tttcgcttag
    10621 cttatatatg tagataatag cacaaaataa aaaaaatgaa cggcacaact atccattgcg
    10681 ccgttcatgt gatcttggtc aaaatctaat gattgattcg gtttttaaag ctgtcataca
    10741 atcatctgtt ttatactttt ttacttttga tttttccaga ccatccgtca tatccaccct
    10801 taagtaaata aatctcttga taacctgctt tacgtaattt atttgctgta cggatgctca
    10861 aacttttctt ttgatcgtaa atatagacag gctggtcttt tcgaatagat cctaatgaat
    10921 ctttcaaaac agtgtaaggg atgtttcttg cacctaaaat atgcccagca tcaaacacat
    10981 ctttttcgcg aacatcgatt acttgtgctt ttctcattcc ttctcgaaat tcttcttctg
    11041 tcaaagtcgt tgctgaacgt ttggccatta ctcgtagata gacttcccaa aggatgattg
    11101 caagcacaat caatagcaaa atgatattaa ttacccataa aaccatgtaa tgcctccgtt
    11161 tttttaagca aatttcaagg ctaatgaggc agcaccaatg acaccagctt cattaccaag
    11221 ctctgctaat ttgatttttg ttgattctct tacttgcggg aaagtaaatt ctttaaaata
    11281 tttctctaca cggctacgta aaaattctcc tgctgccgat actcctccac ctaacacgat
    11341 acttgacgga tttaatgtat tccccaagtt tccgcaagct aaacctaagt agaagcatac
    11401 gcgatcaaca accatcaatg caaacggatc gtcttcttga gccatttcaa agatatcttt
    11461 acttgttact tcttctccgt tgtctagcat tgctttcagt tttgaatccc ctgcgtattc
    11521 ttctgccaag aaacgaccta ggcgtaccac acctgtagcg ctagatacag tttctaaaca
    11581 tccgcgttta ccgcaagtac attcgaaccc ttcaggatcc acagtgatat gtccgatttc
    11641 tcctccagct ccgctcacgc cgtgaatcaa atttccgcca gcaacgatac ctccgccaac
    11701 acctgttcct agagtaacga atacaacgtc tggatcgttt tctccagcac ctttccatcg
    11761 ttcacctaaa gcagcaacat tcgcgtcatt atcgatagcg aatttgattc ctgttcctgc
    11821 ctcgatcagt tttttcacaa attgaacttc tgtccagttt aagttatacg caccaataac
    11881 cgttccagct tcgctgtcca cgcttcctgg ggtacccatg ccgattccga taaagtcttc
    11941 ggctttcata ttatataaat tcaaacgatg attgattgat tcaatgattt caggaacgat
    12001 atgcttacca tcatccaaaa tattggtatc gatgctccat ttttgctgaa tctctccttc
    12061 tggtgttaaa attgcaaatt ttgctgttgt tccacctaaa tcaatcccaa taatcttctt
    12121 gtccattaca aatttccttt ctttcggctt tcttcaattc ggtgctctct ttttaatatg
    12181 cgccacgctg tcatgtatgt atcatgatca atcaaacggc tatcatacag attacgcaat
    12241 tcgatagtca ttaattcaat gtcatagatg cgtgcaccca catagataaa gatgccaaat
    12301 tttttgaaca gctgctgtac atcatataag ctctccatct ttaagcactt ccttcatatt
    12361 atacaagtaa accatatttt tttaaaccca gtaaaataca tattacaaaa agaaatacaa
    12421 aaactagtgt agaaagaatc ctttggtgta tcgaatagct tgtctgttgt ttcggtagac
    12481 ccaaaacatt ccctaacact aatccgccaa ttagcccacc gacatgtccc caaacatcta
    12541 cagaggtatc cagaagacca aaaacaaatg tcaacacaat aaataaaaga aaatgtctga
    12601 ctagctgctg gatcgctggg ttatgtttaa aatgaaaccc taagataaac aacgcaccga
    12661 acaaaccgaa tatcgaagta cttgcaccac cagacagaac attcgcttcg ttaaaagcaa
    12721 agcttgctgt atttcccatt atcccgctga acatataaat caagaaaaaa cgccaatggc
    12781 catagattgc ctcgatttgc tgccccataa aatacagtat cagtgagttc attacgaaat
    12841 gcatcagtcc aaaatggatg aagattggtg tcactaaacg ccaccattca ttgaagtgaa
    12901 caagataagg accatacatt ccggcataat aagggatctc aagtcgcgga aataattcca
    12961 tcatgaggaa gaccgctgtc tgtatcccta aaaaagcata ggtccataac ggtcgtctta
    13021 accatgcacg caattttatt tgctgttgat aattcatgaa aacctcccat ttcattttag
    13081 tcaataaaca attgttggat cttctgatca aagctctccg gttgccattg ttcattcaat
    13141 tgttcacgaa aaacaaggct acatgttttt ttatgaaaat tatttaaata ccggtcgtaa
    13201 taaccaccgc caaaaccgat tcgataaccg gctctattgt agataatacc tggtacaatg
    13261 aggagatcga tcatctccgg caaatagagc ttctctgatt gtggctcaag aacaccaaaa
    13321 ctcgaacgta caagaacgga ctgttcatcc atttcataaa acgccatctg acgtttcggc
    13381 aaagttttag gtacaactat tttcttttgt tctttttctg ctcgttggat aaccggctgt
    13441 gtatccagtt caaattcatt cgataaagtc gtccctatca cttttgctga cttccagtac
    13501 tcgctggcga atagctgttt atagattcgc tgttcttttt cctttttctt gccacttttg
    13561 gccagatttt tcagcttttc gattcctttc cggcgtaatc ttactttcaa ttggtccaaa
    13621 aactcaccct tccttctctc ataaaatggt ataggaaaaa aagaaaaata gcaatcgaac
    13681 tttcaaataa atgcataaat agaataaaaa gaagacctag aaatgattgg ccttcctttt
    13741 ttaatgaatt tcaactgttc agcgcgtgtt tttgtctgat aaaaagtgca tacagaaaat
    13801 atggcgcacc aaaaagggca atcaaaattc ctgtaggtac ttccccactt ggtagaatca
    13861 ctctagcaac catatctgca ctaagcagaa aaatggaacc gaacaaaccg ctcagaacaa
    13921 aggtccagtt attttcttta cgtacgacta gcttcgcagc atgaggagct agtagtccta
    13981 caaaagataa actacctgtc accgctacac taccgcttgc caaagcaaca gctaacaaca
    14041 aaaggatttt ttgactttta gccacgttaa ggcctaatcc ttgcgcgcgt tcttgaccaa
    14101 atgatagcaa tacaatcctc tgtgcataaa gtcctcctag cggaattaag ataaccgccc
    14161 atggaagcaa tgcccaaaca taaggccagc tcgcacccca gattgatccc gctaaccatg
    14221 cattcacaaa tccataattt tccttagata attggatcgt gcatagcaat gttaatgcag
    14281 aaaggccggc attaacggca ataccggaca ataagattcg attcataccc aaaaagccat
    14341 ttcgatcata ggccgcaaga tagacacagc aagcagctaa aacaccacct agaaaggcta
    14401 caactagcaa tagaaacggc agcgaatagt gcgaaaagaa acctaaataa agcatcacta
    14461 aaaatcctgc tccggcatta atgcccaata tactagaatc tgccaattca tttcttgtta
    14521 ctccttgcaa gatataacca gaaacgccaa gacaagctcc tgctaataaa ctgatcagta
    14581 atcgaggaac tcgaaattct ctaacaatca gcgccaaaga agatgattct ttccctatga
    14641 acgtggcaaa aagctgatcc aggtaaataa aaggcgtacc aatcattaaa ctacctatag
    14701 acaggatgac aagtacaaaa agcaagaaat agaacgtcca atatattttt ttcaccgttg
    14761 ttccctcctt acttgtatca gtaaaaacgg aacgccaatc aatgcaatga ctaatccaaa
    14821 aggtgtttca aaagggggat tgatcatcct cgctgtaaaa tccgccaaca taaccaatcc
    14881 accgccaact aaaaaagaga caggtagaac ataacgatag tccgttccaa ccattccgcg
    14941 aacaactggc ggaaccaaca gcccaataaa tgaaacaggt ccgactaacg aaacggcaat
    15001 ggctgctaac actgcaacta gacagaaaac aatcaaccgc gttcgtttag gatgacttcc
    15061 aagagctaat gcatgtgttt cactcaaatg aatcaaagat attgcttttc ctaatgaaaa
    15121 agaaaccaaa acagcaagta tatataaagg aaacaatact gctaattgtt tccatgtgat
    15181 attggcagct ccgcctacaa accaaaaagc aagatcctgc tgcagattaa agaattgggt
    15241 aagtgcttgg ctgatagcag taaaaaaaga actaatagct acgccagcta aaatcagctg
    15301 taaaggattc atgcccattt tcgaaaactg caaaaaacca aatacgagca aagttacagc
    15361 aaaagaacct gccaatgaca ttagaaacac agaaaaatca gaagctttag gccaaaaagc
    15421 aaaactaagg gccattgcca acgaagcacc cgcgttgatt cctagtaagc ctgaatctgc
    15481 taaaggattt cttgtcaccc cttgcatcaa tgcaccgctc aatgcaaaac aactccctac
    15541 aagaaatgct cccgccacac gaggcaaacg aatatttcgt atgatttggt gactttgttt
    15601 tgatctatca aaatcaaaca acgcctgcca aaaatctgct ttttccacag tcgctgcgcc
    15661 atttgaaaga gcaacaaaaa aaataatcaa aagaacaaaa cagaccaaaa gcaggagtct
    15721 atttttcttc atagtcctca taattgatcc tcctgctctt ttggcaacaa atcataggtt
    15781 agaaaaactg gtttatttgt tcttggaagc gtgacaatcg ttgcatcaat ctcaaatatt
    15841 tttttcatac actcttttgt gatcacttct tcaggtgtac cttctgtgat caacttaccg
    15901 tttttcattg ctaataaata atctgaaaaa cgtgaagcat gattaatgtc atgaatcgac
    15961 atcaagatcg ttgtttgttt catttgattg atttcagtca ataactgtag gatctctaat
    16021 tggtgtgccg gatctaaaaa tgttgtcggt tcatccaaga tcaagatatc tgtttcctga
    16081 gcaagcgcca tagcgatcca gacacgctgg gtttgtccac cagacagcgt tgcgatatcc
    16141 cgcatacgaa atccagctag acctgtagca tccaacgccc actcaattgc ttcatgatct
    16201 tgcgtgtcca tgccactgaa cagtttttga taagggtatc gtccgtaaga tactacttct
    16261 tctactgtca tacctgtagg gtgttcacta gtttgtgcta ataatgcaat tttttttgca
    16321 agttcttttg aactgtatgt agagatatca gagttattga caatgatctt tcctgatttc
    16381 acaggtagta tacgggtaaa acttttcaat aaagtagatt ttccacttcc atttggccca
    16441 atcaaactag tgattttccc ttcaggaatc gtaacagaca tgttttcaat gatttttttg
    16501 ttttcgtagc cggctgcaat gtcagctgct cttaatctcg tcatttcacc catcctttcg
    16561 ctgaaaatca ttctcaatta tgacgctgtt tcaacatttc gtcaagcgct actttcatat
    16621 ctatagccaa atgaagcggt tgacagcgtt cgaaatatca ggaataataa ccaatgagaa
    16681 agattctcaa ttatgttagg aggaaagaaa tgaaattaaa aacaaaagta ttcacattat
    16741 ctagcatatt gctgctggct ggagtactga gcgcttgctc cccctcttca gataaagaaa
    16801 cagctacaac tgccaattcg acgacttcat ccaatagtac tcatacagtc acagatacac
    16861 ttggacataa agtagagatt ccaaacgcgc ccaaacggat catcggcagt tacttagaag
    16921 actatctgat tgcactaggg gaaaaaccta tcgcccagtg gactgttggc agtggttcca
    16981 ttcaagatta tctgcaagat gatttaaagg atgtcccaac gatttcttat gaccttcctt
    17041 atgagaaagt gttaagcttt gagcctgatt tgctgttgat ttcttcttcc gctactgtcg
    17101 aaggcgggaa atacgaacaa tatagcaaaa tcgcgccaac ttatgttgta aaaaacggaa
    17161 cagatgtcac atgggaagaa caactaaaag atgtcggcaa agctttggca aaagaaaaag
    17221 aggcagaaaa aatcatttcc gattatcaaa aatccatcaa gaaaactcga gaagaactag
    17281 cagataaaat cgagaacaag acagctgcag tgttgtgggt gaccaacaac tcagccttta
    17341 tggtcagcaa aaatcggtca agtggaagaa tcgtatacga cgatctggaa tttggtgttc
    17401 ctgatctagt ggaagaggtc tccaaaaaag ccacttctga ctggtctgct gtttcctctg
    17461 aaaagctagc tgaattagat gctgactaca tcatccttgt gaacagtgac aaagatgctg
    17521 ccatgttcaa tgaaccaagc tggaagaatc taaaagcagt gacagacaat catgtactgc
    17581 aattcggacc tgaatctagt tggctatata acggacctat tgccagtcag gaaatggtca
    17641 atgatatcaa agaaaaacta aattgatttt aactaaaaaa agcatcttca cgattagtcg
    17701 tgagatgctt tttttcttat taacaaacta gacgcataga ttttattctt cttttgaagc
    17761 aaccctattt tttactcttt cccatttttg aaaacgatgg gcatatagat tttcttgatc
    17821 cgttatcccc tcatactcct ctataagggc gaagtctgaa aaatcaatat tcccaatata
    17881 cgtatcgcct tcaaacgtgc tatcgatcaa tgttcgccag atccgaccag tgtaaggaag
    17941 aagttcttga aaaatccgac taccacccga aacatagata tcttctgaga tcgtttgact
    18001 atacgacaaa agttcatcga tcgaatgcat tacttccgca ttttctttcg tgcgaaaatt
    18061 tggttgcgtt gtcaacacga tgatatggcg ataaggaagt gaaagatccc ccattccttc
    18121 ataggtcttt cttcccatca ctaaaattcg atccattgta tgttcgcgaa agaaacgcat
    18181 atcatttggc aatcgccatg gtaacaaacc atttttgccg atcagaccat ttttatcttg
    18241 tgcccacatg gaaataaaca tattatccgc tccttatgat tttagatgtt ttgagataaa
    18301 aaaagtctca attctttgac cagcaaggaa ttgagaactt taaaaacatt attttgtttc
    18361 acggtgcaaa gtaactttgc gttcgcgtgg gcaatatttt tgtttttcca aacgatcagg
    18421 attgttacgt ttgtttttgc ttgttagata gttacgttct ttacaagaag tacactctaa
    18481 agtgatgttt acgcgcatgt ttttccctcc catttttaca agaatcctct aagatgactc
    18541 tcagacttga ttatcttacc atgatttttt ctagattgct agtgtttttc acgattttgt
    18601 taagtaaaac tacttgacga taaatacaac ggttttactt ctcaaacaaa atctttttct
    18661 ttttgtataa atacccttac acttgtttat atagattatg ttttttaata aagggggata
    18721 ctaatatgcc atggaacatg aaggactttc ctgcatcaat gaaaaactta gataaattga
    18781 caagaaaaaa agcaatcgac atcgcaaatg cattattaga tgaagggtac cctgataacc
    18841 gtgcgatccc aattgccatt gatcaagcaa aaaaatggta tgaagatgct agtgaatcag
    18901 aaaaacgaac tttcgaaaaa gaaaaaaatc caagcaagac ggatgaacac gatacgaatc
    18961 ctcgtgctgg aaaactgctt gattcagatg tgatcgtaga atatgaggaa gaacaatgga
    19021 tcgtgaaatc taaaggggca aaaaaggcaa gcaaccattt tgatacaaaa aaagaagcaa
    19081 tcgaaaaagg aaagcaagta gcacaaaaca aagaatcaac tttgttcatt tataaaaaag
    19141 atgggacaaa agaaaaagaa atcgattatt gaacatatgt aaaggggttg tgacgaaaat
    19201 cgtgtcacaa cccccttatg ttcgaaacaa tggtgttcct ctgccaactc cgaaccataa
    19261 gtcataatat ctataaaacg ggaagagctg ttttttgaaa ttgtttctta tcactcagtg
    19321 ttaaacggta ggagaaaacc tcttttttat tgatatttga ttttcatcaa acagtcacat
    19381 agtcgattgc ttctgcttgc ggaatcgttt catctacaaa gtagacttta taccatgtca
    19441 agaaactgaa gattgtccag atttttcttt gctcttcttt acgtccttcg tgatggtcat
    19501 ctagcaactt catgattttt tcttgatcaa aaaattcttt tgcaaaatct gcttcgaata
    19561 atgctttgac ttgttcgtag ccgtgttctt ctttcaacca agctttgatt ggcacaggga
    19621 atcctaattt cacacgattc gaccattctt ccggaagatg tttgttcgca gcttgtctga
    19681 aaatatcttt tgtgttgttt tgattcaata aaaacttact tggtatttgt tgagccaatt
    19741 tcatgacttc gatatccaat aaaggtactc ggacttccaa agaacttgcc atagaaagtt
    19801 tatctgcctt taacaagata tcttttggca tccattgatg aagatcgaca tattgcatct
    19861 tattgacttc atctttgatt ccttctgttt tctcgtaatg aacagtcatg atttcatcca
    19921 cagatggtgc tgtttgatat tttggtgtta acacttctga tgcttctgat tcttcgaaca
    19981 ctcgagcatg accgataaaa tattcttttg caggagcaag tgattcatat aaatggatcc
    20041 gtccatggaa gtttttcatt tttccgattt tacgtcctaa attatagcgt gttccttttg
    20101 gtaatttttt caatccttga gcaatcacac ggatgaattt tgaattcgta tggaatccat
    20161 acgtttggta tcccgcaaac agttcatccg ccccttctcc agattggact acccgcacac
    20221 tttgtgaagc aagatttgct aaaaagtaca aaggtacgca agaaggattt gaatcaggtt
    20281 catctaagtg atactggatc aaagggaatg ctttgaacga catgtctccg tcgatcactg
    20341 ctgctgtatt atctaagtcc aatagttcgg ttagttttct ggcttcaatc gcttcattat
    20401 aggttttatc atcaaacccg attgagaaag aatgatctgg tttcaataca gaggttacat
    20461 aactggaatc aaccccgctg gataagaaag aaccaacttc tacatcactg accgtatgtg
    20521 cttcaataga agcttgaacg gtttcgtcga ttttttcgat ccattcttgt cgggaatgcg
    20581 tttcttttgt ttcaaaatct gcatcccaat attgctgaat gtctattttt ccatctttat
    20641 acgtataata atggccttca ggcaaacgat agacatcttt gaagaatgtt tcgccgttta
    20701 atggagaata ttggaaagtc atataaggtt tcaacgcttc tttgttcaat tctttgttaa
    20761 aatcaggatg aggcagaaaa cttttgattt ctgaaccaaa cataaatgtt ccgttcattt
    20821 ctgcataata atatggtttg atcccaaagt ggtctcttgc accgaataac tcattctttt
    20881 cattatccca aatagcaaaa gcaaacattc cgcggatctt ttgcaataat tctgttcccc
    20941 attcttcgta accatgcaaa aggacttctg tatctgcatg tgtttgaaaa acgtggccag
    21001 ctgcaatcaa ttcttctctc aaaggctggt aattatagat ttcgccatta aaaataatga
    21061 tctttgtccg atcttcattg taaataggct gagtaccgcc ctctaagtca ataatactta
    21121 atcttctgaa tcctaaagca acatgtttat cgatatattc tcctgaacta ttaggtccac
    21181 ggtgaacgat tcgatccatc attgtattaa taattgtttt tttgttgtcc ttgtcgttaa
    21241 caaaacctac aataccgcac atttaacttt cacatcctaa tataaaatta ctgtttattt
    21301 cattcatatg catcttatca tagaataaat agcggacgaa tattttctct cttacaattt
    21361 cgtcacgtga aaacaaaaga tgaaagtttc tgtttttaca gaaaataaag tcccaatatc
    21421 taatttacca tgttttcttt tttgaggcta ccttattctc ttcgtgttaa aaaaatatta
    21481 gtggatgcta tttccaaaaa aaccagcaaa aaaagacacg acctaaagat cgtgt
//
LOCUS       DAJSAW010000045        21458 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJSAW010000045 DAJSAW010000000
VERSION     DAJSAW010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.045"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(136..1599)
                     /locus_tag="ONT64_001921"
     CDS             complement(136..1599)
                     /locus_tag="ONT64_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR2303332.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(1663..4680)
                     /locus_tag="ONT64_001922"
     CDS             complement(1663..4680)
                     /locus_tag="ONT64_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2303333.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(4831..5670)
                     /locus_tag="ONT64_001923"
     CDS             complement(4831..5670)
                     /locus_tag="ONT64_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2303334.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            5815..6060
                     /locus_tag="ONT64_001924"
     CDS             5815..6060
                     /locus_tag="ONT64_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303335.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            6123..6545
                     /locus_tag="ONT64_001925"
     CDS             6123..6545
                     /locus_tag="ONT64_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303336.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(6639..6956)
                     /locus_tag="ONT64_001926"
     CDS             complement(6639..6956)
                     /locus_tag="ONT64_001926"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303337.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(6983..7189)
                     /locus_tag="ONT64_001927"
     CDS             complement(6983..7189)
                     /locus_tag="ONT64_001927"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303338.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(7170..7352)
                     /locus_tag="ONT64_001928"
     CDS             complement(7170..7352)
                     /locus_tag="ONT64_001928"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303339.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(7366..7716)
                     /locus_tag="ONT64_001929"
     CDS             complement(7366..7716)
                     /locus_tag="ONT64_001929"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303340.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(7742..8044)
                     /locus_tag="ONT64_001930"
     CDS             complement(7742..8044)
                     /locus_tag="ONT64_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303341.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(8047..8280)
                     /locus_tag="ONT64_001931"
     CDS             complement(8047..8280)
                     /locus_tag="ONT64_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303342.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(9266..9588)
                     /locus_tag="ONT64_001932"
                     /pseudo
     CDS             complement(9266..9588)
                     /locus_tag="ONT64_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9711..10826)
                     /locus_tag="ONT64_001933"
     CDS             complement(9711..10826)
                     /locus_tag="ONT64_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR2303343.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(10832..11230)
                     /locus_tag="ONT64_001934"
     CDS             complement(10832..11230)
                     /locus_tag="ONT64_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR2303344.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
     gene            complement(12259..12645)
                     /locus_tag="ONT64_001935"
     CDS             complement(12259..12645)
                     /locus_tag="ONT64_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCR2303345.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(12642..13163)
                     /locus_tag="ONT64_001936"
     CDS             complement(12642..13163)
                     /locus_tag="ONT64_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR2303346.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(13165..14199)
                     /gene="sppA"
                     /locus_tag="ONT64_001937"
     CDS             complement(13165..14199)
                     /gene="sppA"
                     /locus_tag="ONT64_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR2303347.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(14232..15647)
                     /locus_tag="ONT64_001938"
     CDS             complement(14232..15647)
                     /locus_tag="ONT64_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR2303348.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(15616..17769)
                     /locus_tag="ONT64_001939"
     CDS             complement(15616..17769)
                     /locus_tag="ONT64_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HCR2303349.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGE
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            18027..18263
                     /locus_tag="ONT64_001940"
     CDS             18027..18263
                     /locus_tag="ONT64_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HCR2303350.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            18282..18497
                     /locus_tag="ONT64_001941"
     CDS             18282..18497
                     /locus_tag="ONT64_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HCR2303351.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(19200..19952)
                     /locus_tag="ONT64_001942"
     CDS             complement(19200..19952)
                     /locus_tag="ONT64_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2303352.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(19967..21214)
                     /locus_tag="ONT64_001943"
     CDS             complement(19967..21214)
                     /locus_tag="ONT64_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR2303353.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(21290..21436)
                     /locus_tag="ONT64_001944"
     CDS             complement(21290..21436)
                     /locus_tag="ONT64_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HCR2303354.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
BASE COUNT         6308 a         4110 c         3160 g         7880 t
ORIGIN      
        1 atatttaata aactacgttc tagatagata cgagatttca aattaaaaaa cgaaaatgag
       61 agatacaaat atatcaaata tttaaaaagt aaccacattt tctataccac tccttatcag
      121 aaacagcctt attctttaga ataagactgt ttctgggttg attaatttgt cttttgttaa
      181 actaccatct aaggcaagtt ctaaatgtaa atgtggtcct gtactatttc ctgttgaacc
      241 cacataacca ataatttgtc cttgttcaac tgtttgtcct acgttcacgc aatattcctg
      301 ttggtgcgca taaagagcca cacttccgtc ttcatgctca attgtgacat aatttcctga
      361 caaagtatga tacgaagctt ctatgaccgt tcctgccttg ctcgcataga ttggttctcc
      421 ttgattggct gccatgtcta atcctctatg aaattcttct cctcgccaac cgaaggaact
      481 agaaattgaa taatttttta gaggtaccat atagccttct ttgcttagtt gtggaccagc
      541 tacttcttta tcatatttga ttaagtcata ggtttcgatt aacccattta attttttatc
      601 gtagctagtg tctgttgcgt atcttcctgt taaaaattta gtcgcttcct gataggtttt
      661 tgccttactt ttccaaacac catcataaaa agaggtattt ccaactaagc cttcttttaa
      721 cagtttggca tagtctttta agctttcttc atagctctca tattgccgaa aagttgcttg
      781 ggtcgtataa agtgtcccat tgcctaaatc ttcttgtgtc gcaaaagaga cgccctttcc
      841 gttatgtgtg cctttgattc caaatagatt gtagtttggt gcctgagcca attgactttg
      901 tccgcttgcg gattctaaaa tggcttgcgc gatcataaca gaagcgtata aatcattttc
      961 ttgtccaatt ttacgagcag attcaccgat tttacggata aacgtttcta cagacgcgtc
     1021 tttttcaaaa tgaatagtcc cttcgttatc ttgtgtgctg gaagggacta attcttgtat
     1081 cggtgcgctt ggtgcttggt ggtagttttt tgtctcttct gaaccgattg attgtgcctg
     1141 cggtacgtca ttcataggta cagtcacact ctgttcaggt gccttttgtg ggcgttccgt
     1201 ttttgttggc ggttccgctt ctgtttcggt acttggtttt tgaggagtct gtgagctttc
     1261 atcagaaggt tgctcatttg gttcttctga cggttcctcc tcaggttgtt gattgtcttc
     1321 tggtttgtct ggttgattcg gtgtttctgg ttgttcttca gaacctgttg gtggagcgtt
     1381 catttctgga ttatctggtg tatctattgt atcttcagtc ggtgtttctg gttgattttc
     1441 ttctccagtg gaatttgttg tcgtttcttc agtgatcgtt gcagattgcg aatttgtcag
     1501 ttgattgttg ccttgttcat tagccaaaac cgctggcgta tacccaaata cttgacctac
     1561 taataacaaa gcagataaac atttactcgt ttttttcaca agattgcctc cattcttcaa
     1621 tcaatcatct agagacagct gattgctgtc tctagtcata ctttaattgc tttgtttcat
     1681 tttacgacga accagaacag cccccacacc aacaaaaaga aacagtccca atgcaattaa
     1741 cacatcgttc actgtttctc ctgtttttgg taagctttgt tttggtgtgc tcgtcggcgt
     1801 ttgtttgaca ggggttgtcg gcgtttcttt tttaatgcct tttgctgtca atgtttgttt
     1861 ttcattcttc aaatcaaagt gacgtgcttc ttcctcttta tggtctttat ccttatagac
     1921 aatatgagtt gcgaccaatt tctccccgtc ttttaacgtg cctgctttgt agtcaaagga
     1981 tactttaaac tcttcagacg ctttagaaac tttacgtgca gtttctaccg taccaacgac
     2041 tgtatctttt ccatttttat caatcttatg gaattgagtc acccagtatt tggtttcccc
     2101 taccgtagat tctggaaatt cttgcgatac atgatcgatc aactcttggt ctttgtctgg
     2161 atcaaatgtt tggtcgccat tcactgtaat aaagcgtgtt tcgatttttg gttctttcgc
     2221 agtcactgtt tgatctttat tggttaaatc aaaatgacga gcgaattctt cttcctcttc
     2281 tttgtcgtta tagacaatgt gcgttgcgac aatcgtgtca ccgtctgaaa ccatgtctgc
     2341 tttgtagttg aacgtcacat caaatgtttc tttttcatcg gtcacttttc gtgtttcttt
     2401 taccgtatct aatacagtga cttttccatc ggcagtaatt ttatggaatt gcgtcaccca
     2461 atatttctct gtcccaaccg cagatttagg gaatacttgg gacactttat ccactaattc
     2521 ttggtctttt ttcgggttga aatcttttcc acctgtcgtt gtcacaaaca aagtagaaat
     2581 ttctggttgg tcttcttctg gaaccacgat ttcttcgttt ttcacagtaa ttggttgtac
     2641 ttctccgtct ttgaccgtta ctaaaatcgt ttctgtttgt cctgacgctt gcccataatg
     2701 ctcagggaac acaatttccg taaatttata cgtaccgtct ggtaatttta gtttttctgg
     2761 ttcttctttt gtttcataca caaaggacac atttttatta tgtgcagtca taccttcaat
     2821 caggaatttc gctcctggta attgtagatg attgattgaa tcgactttat tgaagtctac
     2881 ttcacccgtt tttaacaagt tgtcaatcac atctaattca aacgtaagca caggcgtcgt
     2941 gttatcgcca taggccacag agaacgtggt ttgttcttca gaaggttgac ggtagttgtc
     3001 tggcacattt ttttcaatca attggtattt gttttgatcg ccattgtaga attttttgtt
     3061 tgccttccag tgtccgtctt tgtcagtttt tactgaatcc aagacttctc cttttttcac
     3121 aaggactgtt tctttatctg gcgcagtaat atctgttttg gcgataatat caaattcgat
     3181 attttctgca ggaacggtat cataaacagg tttgccttga ttccaggatt cttccgcatt
     3241 atagacttct ttgactttct ttccttctgg aatgaactgt tgtaagtcat tttttgcagt
     3301 taacgtcact tctttgtttg gttcaatcgt aatcgtatga gtttgtttgt ctagaatata
     3361 cccagctggt gctttgactt ctttgacatc caatttattt tgaacatatt ttccgtcttt
     3421 ttcatagaat gcagggattt cttttaaggc gatttcccct ttggcatccg ttgtatattg
     3481 ttttgtttct cccgtatcat gacgtttgat ttcatacgtc acatttggaa cttttttccc
     3541 tgtgacagcg tctagtttgg ttagtttgaa gtcgccattt tttagaactt taatagttag
     3601 tccagctcta ttaggatctg gcacataaag ctttcctaga ttttgagcaa caggatctac
     3661 ataggcaaaa cttgaaccta cccctctaga atttggatcc agtgaagaaa cagaaattcg
     3721 tccgtcattt gagttttgat ttgcttttat aaacaaacta tttcctgatt ttcttacttg
     3781 aacgttagct gtgttttcaa caaccgaccc atcccattga gaaaacacac cttttgtatc
     3841 cttaacttct acttcttccc ctactttaac cgtcactgtt tggctatcaa aagaaggatc
     3901 ttcaagtaaa ttgtttattt ttctatctac agtctgtttg aaagcttcat aattagattg
     3961 tccaatagtt ccataattaa caaatgaacc acctaaaact tcccaaatat atgtttgtgc
     4021 taatacatag ttatccattg aaccactaga atagtagcca aaataaccaa ttaaagaaat
     4081 cttttttcta atagcacttg tcgtaattaa attttcgagt ggtgtcacat catattgaat
     4141 acccgcactc gtgattgcgc cttcttgaat gcaaaaaaca tcttctccgt caatttgcca
     4201 gcgaaccatg taatcatgag tatacattgt tccttcagga gtaattcgag tcatattcac
     4261 atctttacga ccattttcag tcaatgttag attggtagca cgtgtctgtg cattcttttc
     4321 caagattgct tgccattctt cagattcttg cgccaatcgt tcttgttcag aaaaagaagt
     4381 cgctttttga atatcttcaa tggttataaa gtatttctca taaccttcag ggacctcttg
     4441 agtcattaca ttgggcggag aaacatcttc tgtcgtgtcc tgcgtagttg tctcttctgt
     4501 tgtttcactt gtggtttcag tactgatcgc ttcttcacta ctttttgatt cttcagtctt
     4561 cattacttct tcagtagaat ttgattcatt tatcgtttct gcaaatgccg ttacagatcc
     4621 taacatggga ttgagaaatg taacgaaaat tgccaaccaa gttgccattc ttttaaacat
     4681 atttttttcg ttcctttcat tttatttttc agtcttctaa agtaatttct tatggacttc
     4741 gacaccgaaa catttttatc ctcctcaaaa caaaaaaaga ctagaactta taattctagt
     4801 cttcatcaac cacttacttt tcttttattt ttagtagaaa tctacagtgt gtttttgtga
     4861 cccgtcactg taaataataa catccaccat aaaacctttg ttgtaccact gcgaatttgg
     4921 atcgctaacc tctgcattag cccaatcata ggcttcatct acagtgttaa atacctttcc
     4981 actattacca atctctccaa ctacaattgt tggctcttca ggtgtcgttg tgccaccatt
     5041 tccgccttga tcgcctccac caggattgct tggttcttca ggcgttgttg ttcctccacc
     5101 tgtgcttggg ttagaaggag ttgtttctcc tccagtattt ccaccattac ctggttgtgt
     5161 aggtgtttga gtagtgccac cattgttttc tggtgtactt ggcgttgttg taccaccagt
     5221 tgttcctgtt tgatctccag ctggtgttgt tggtgtcgtt gtatttggtg tcgtttgtgt
     5281 atttgaagtc gttccttgtc ctactggttg attcggatca actttgtctt tctcttgaat
     5341 cactgtttga gccacagttt cacctgcgct gttttgtaca cttgctacat tgccattaaa
     5401 tactaattta tttcctgcat agattaaatc cacatttgta atcttattca tgtccgctaa
     5461 tttttggaca gtaatatttg ttgcttgact gattgcgctt agggtatctc cccagacaat
     5521 tgtgtattca ttccctttaa tttctgcttt gatttgttcc gccgaacgag gtgtccaaac
     5581 tttgttttct gctgcttcag cattcgttcc cacagcaaga ctaactcctc cgactacgat
     5641 tcctagtgcc aatactttca acattttcat tgtttttcgc ctccgatttg aataaattta
     5701 tttacaaaaa catagtacca aacttatgtc tatcagtcga tatatttttt gtagattttt
     5761 ttattttttg atatactaac tttggtacta aaataactaa aaggaatgtc acacatgcaa
     5821 cttaataatt taaatgaaat acatgattgg gctcaaaata atttacttaa tcgtaaacaa
     5881 gctgccaaaa tcactggaca aaactatgat gctttttcta gctctttacg tagaaataat
     5941 atcaaaccat ttctagaaat aggcgacgaa cctggtcctt ccatcattcg cttatatcta
     6001 agagaagata ttgaaaaata cgcaaaacag ctcgaagaaa aaagacagaa acgtcaatga
     6061 ggcgttttct gtcttggatt tacaagtaaa acaataataa acacttttac aacggaggtt
     6121 ctatgaataa gaaatacatt gtttcagttg gttcaatcgt actgattgta ctcgctatta
     6181 ttgctttttc tgtatccaac aaaacagaaa cacagccaca aaatacggtt caatcgacta
     6241 ctacaagtca atctagcgat cattccgtct atccttcact aaatagttta aaagaaacgc
     6301 atggacaaga tttaattgat cttggttctt ctcaaattac tactgtctta tttgaaaaga
     6361 aaaatattgc gtttattact cctaaagaag gaactgacct tgaattttct atcaaagtaa
     6421 caaataccaa agaaaattta ttctccattc ctgacattca atatacccct aacaagttaa
     6481 agattgggga tacgtttggt ttggctaaag taaatgaaca atactttgct tactcactga
     6541 aataagtttc taatagcaag aggaaaaacc ctcttgttat ttttatcaca taatcatttt
     6601 ataaaaacgc ttcgaaaata tcaacatttt tgaagaaact atttttctaa tttctcaatt
     6661 tcttccaagg gattttcgct aatgacccat acaggcacag attgctctcg aggctcccct
     6721 tcttctaacc accaacctgc ataatgaact tcttcctcct cagcattacc atgataccaa
     6781 ccaacaaagg attcatctac aacgaatgaa atttcttctt ctggatcaaa taattcattt
     6841 ttccatgctt caattgcttg ttctttggtg tacttttgtt tgttaaatac accacaaatt
     6901 tcgtcatcta cataaaaaat ttcaaaatca aaattactcg ttccttttac tggcatctta
     6961 acttttcctc ctttgactca cttcaataga tataccactc atgttcattc cattgtttac
     7021 tagttaattg gagttttatc ttagggcttt gtgcactctt tccaaaaact tgagcgtcgt
     7081 ttttttcttc taaccaaaaa aggtaatttc ttacattcgg tactaaatgt ttttcctgca
     7141 tttcttcatg aaaaacatat aattctgctt catttttcaa ccaattcatt attcattccc
     7201 tcattcactt cttcataccg ccattcagat agtgagattt ctttttgttt ctccacttgt
     7261 cctataaaaa tcgctgtatt caacaagctg atcacactta ataataaagc aatgaaacta
     7321 atggttacac ttactctttc acattttgtc attttgtttc cttctttact cgtttgttat
     7381 ttcttgtaat aggtctaata actccttcac cgaaatttgc aaatggttcg caaaaatttc
     7441 aataaaagca ctcgcacaca ttaccagacc ttcaactgat atatcactgc gacaataaaa
     7501 ttctggagaa acactgattt ctgattcttt cttacaattg aacgaaaaaa ctatttttga
     7561 atcaaattcc ggtaattctt ccacaactat gttttgggat tctaccaaga aagggagtgc
     7621 ctgttctaat gcttcttctg gtgtttgagc agatagaata attgcttctt gttcttttat
     7681 ttcattgttc tcaaatactt tataactttt tttcattgtt agttcctctt tcattcctct
     7741 ctcaattagc taagccgtta ctggtacgcc attctctaaa tttagtatcg ctaatcctta
     7801 atgctcgtcg aatatcaatc attcgataac ctattgcacg aagttcttta tatcttttag
     7861 cagttagcgt ctctaacgtc atttcaggca atgggttcga atcaaaaata gaattcgaac
     7921 cgattatttg ggccaattgt tgtacttgta acagctcttc tgaacttaat tcagtgattg
     7981 ctcgattgcc tatcattgca tacattttct gtcgaatata cttcttttct tgtttagaca
     8041 acatttctat ccactcctat tttcattatc atttgttacc tccacttcag taaatccaca
     8101 aataaatgaa ttcatattcc aagtaccatt cccttcaact acaacttttc tcctgtcctc
     8161 tttcggatat tcaagaatca gaccgtttac caatatgttt ttcactacta ctaatttgtt
     8221 tgtataccgt ggaattgctg ttccgatata cttcacttta tttcctacgc taattgtcat
     8281 ttcctcactc ctattctttt ttatccaaaa ctttttcctg tcctattcct gccaacgaat
     8341 atacttcact gcatgcttaa acaaaacaaa atgttgccct gttttatttc cgtcttttac
     8401 ttgttccaca tacagttcat atttggtcac accttttaaa acgccagata ctttttttca
     8461 tccattgtca caattagttt tttcatcaat cgattgatac tgcactgctc atttttcttg
     8521 ttcatatagt attcctttct ttttcaaaac tagcctcttt tgtttactag ttttcatcaa
     8581 tcatcaacga tttaacagat tttttctttc gcagggtttc tagcgtcagc aaccccaaac
     8641 tctaaaaaag tgcctttgct cccttgaaaa acgagcggac gattgtactc gtcatcatat
     8701 gtttttcata ctacctgatc cacgcaaata accttttttt gttctgtgcc acacccatcc
     8761 atacgtatcg cttgagattc accatacttt tttaggtggt ttatccttcc taagtccctg
     8821 ttagacacgc tgattaactt agaaagtcct gtttgtatcc aattttcaaa gatctcccaa
     8881 aagaaataag taggtttacg tgtatcgctt ctgttcaaag tacctgtttg tacttgagta
     8941 tttctactcc cctcgttcaa gggattcctt ccaccagcta gcttttcctt tcatcatcaa
     9001 atgttcctga aaagcattta cgtttcgtgt ccgtgtgctt acttattctt ttttcgatag
     9061 cgattatccg ctcgctactc gctattttat actttgtttg ttagctcaaa taaaaactaa
     9121 caaacaaaaa aggctagttt ctttccgttt tattttttta actgtcgata aatagttgtt
     9181 agtttaattc caagttctaa aaccaatacg cccaacaaaa ccacaggaaa aacaattggg
     9241 gcaaacaagt aaaatcggat agttttcatg tcttccctct ttctttttgg tgtcgctgtg
     9301 gtcgttcttg ccgttcaata cttcgataca ttttttattc attatttgct cgttttgttt
     9361 tcctctggac tgcttttctt gctttttgaa tactttatac gcttttcgtg cctgaattaa
     9421 taggtcttgt cggcgtttca ttcgtgagaa aacaatattt gaaaccatca aatcctggtt
     9481 cgaacaacca cgattcgcaa taattttccc atcaaaaaaa atagttttca cttgagaaaa
     9541 aaacagttgg aaatcttgtt ttgttgctaa tttttttgtc aaaatcactg tgcttttccc
     9601 tgttgaatct acaatgattc ttcgaacaat ccgattcgat aacttccaac gttgccgatt
     9661 aaacaatgtt acttcacaac aaatatgacg taagtgatta ggggtcattt tcatgtgtcc
     9721 aattcctcca accaattatg catcgaaatt ttgggcaatt gtgacagccc ataaatctgc
     9781 gtttccaggt cttcataact acaagaacgc ttggcttgaa gtaatgaata ttctgcttga
     9841 tctgatagtt ttgttgccat tggatctaga ttcaattctc ttacacaaca ttcccaaaaa
     9901 ttcaatagcg ctctaaaata aaatcctttt cgattctgaa ttgcttttcc ttcatgacga
     9961 gcaatttttt cattttgaat acaccgttga tacgttttgc ttaaatcttc tgcaaatgtt
    10021 tcccctaaaa ttcgatactc gcttccatca gataaaccat gtgtttgatt atactttcgt
    10081 tgccgctttt ctataattcg tttactgtcg tatatcatgt ttagctgctc tttcgcttca
    10141 cgccattcag ctgaactgcg aacaatccat gtcaaaacat ctttcggaac aaacgtactt
    10201 tcgctgtaat gagcaataaa ctctttttgc caatcatcag ccatttcaac aggactagtc
    10261 gtatcgattt ttgtatctag attttctgta tctagattgt tagaagaatt attaaataat
    10321 tgtgcagaat tttcacatgg atttttttcg tcaaaatcat cgaaaatcgc taaattatca
    10381 acagattcct cctcttcttt tccttgtgca gaatctacac tggttgagtc aacaggacct
    10441 aatgaataca catcattttc tgtcgtatct aattctccta gatacaaccg agctttgttt
    10501 ctttttcctg tttgttgatc aaatcccata tcctctattt ctagtagctc atattctttc
    10561 aattcttttt tgattgtatt aacagaacgt ctgcaacaat tcaatgcatt ggctaattct
    10621 tgttccgtaa attcaaaata aacaaaacct tgactatcta cttgatttcg ttgaatggct
    10681 aaatctaagc gtgaccgaaa aaccatatac gcgatttttg ctttatcaga caatgagcga
    10741 tatttttcac ttgtaataaa tacctttgga aattgatagt ataattcatt gtatttacga
    10801 gttgcggtaa atctttttgt tgccatcgtt attacacctc atcttgtcca tatattaaga
    10861 aagaattttg gtgttggtag acacgaacat cttccattgt agcaatcaat tcttttagat
    10921 aaattcgacc gaattcaatt gttagtaagt cgtaatcttc taattcattt ttgatttctt
    10981 caatttcttc ttcagacaca ttgagtaaaa tggctaattc ttgattggta taagaaatat
    11041 agacatgttt cttttcatca atccgttttt caattaagga ttcttctaag tactcattca
    11101 gtaagctata ggcgtatttt gcttgtgcag tcatcgcata atattttgac gattttataa
    11161 agaatagttt tggcatatga aaagttgctt gtgtcagcac atctgttgcg cgaaatttgt
    11221 tttttaccat attttttcct cctagaaaaa cacgcaaaaa aagctctgta taaatcgaat
    11281 tacacagagt ttcttcttgt tgatatttaa tttctaaaat acgtttcggg taaaaactaa
    11341 acacttacta atatttagat tgtagaatct tcgccttcat ttattcattt taaagagaaa
    11401 aagatcaagt gaaatgtgcc caaaagtttg attctattaa tttttaaaca ccattcataa
    11461 tatatttcct ataatgtaaa aagcgtcgat acttattaaa gcatcgacgt tatttttata
    11521 gtaaatttat caaaattttt gccaacattt tttccgattt ttttccctat ttctatcttt
    11581 ccttatcttt cttagttaat attatttgat gttaatatat cactactaat attaaaaaac
    11641 attgatttat cataggataa agagtgatag tttaatactt aaagagacat attaatttta
    11701 tttaaaaatt gacattaatg actcttaatc agcgggtcgc gggttcgagc ccctcacggc
    11761 ccattgggtg ccaaacccac ggaacaggta aatgttggtg cgaaagcatt tcgaaaacat
    11821 ttacgctgtt ctttttttat ttttcaaaat aactagggct ttttattttt aactcaaagt
    11881 ccactgtagc cctttggact ttgagttaaa aaagaagcat aattatgaac agtaaagttt
    11941 ctaatataac aataattcga gaagctttgc aaaatcatgc tgccaatgaa ctctctaaac
    12001 atataggatt aaatctaagt actataaaag gttaaaaagt gaagaatatt taatagaaaa
    12061 actaaattta cttgctgctt tccccttttc cattaataca acaatcagag agtgtctttt
    12121 tttcgaaaaa attagaataa ataaaagaca cgtagttttt ttatctacat gtcttccatc
    12181 gactacaagt tatcatttct caaaacgtat ttagctttta attgattaat cgtttcacgt
    12241 tgtctacaca ttgcctaatt aagattttct tattagaagc atcaatcccg caataatacc
    12301 taaaactcca ggaattaccc acaataatgt taaaagaaaa aatggaataa tagataaaat
    12361 gagtaaacta ataccataga ccactgtttt ttgatttttt cttactaaaa aacctattat
    12421 gaaaatcatt atcgaaaaaa ctagggtaat tcctccaaat aaaacaacat tcccgcttag
    12481 attgactaac atatctatat ccttactggt tagttctgta gtatttaaag tagttgaatc
    12541 aatagaagat agaaacatac cacccatcaa tgctaaaaga ctacataaaa tccctgttat
    12601 tccactaatt aaaatcaaaa taaactctgt ttttctctta ttcatttaat tctctccaga
    12661 ttaagtgttt tagaattaga aattttttga ccatagttaa tagcttctaa tgaacgaata
    12721 acaaccactg acgtgttaga aaaataatcc ccaatatgtt gtttcttggg cgtgaaagca
    12781 gccaccaaat acccaatttg aaatacaaaa ttttttaata taaacctaca tatacactca
    12841 cgaataaaga tagtttgcca acttagctct ttttcctcaa agctcactac tcttatccca
    12901 aaaatcattt tcccaattgt ttgtcctgaa ttcagcttag tcaaaagtac aaaataggtt
    12961 aaataaatag ctaatgacgc tataccataa aaagtaataa tggatttgtc agattccagt
    13021 cccatgattt tgtaaaagag acctatagta gcatgggtga tgatgctaat acaaattaaa
    13081 tcaataagaa aagcaaaaaa tcgtaaccaa aatcccgcaa atacataatt aggcaaagtt
    13141 tcaatgccac tcttcttatt cattttatag ccctccatag taatacattg gttttggagt
    13201 atcagaaaat ccaagttttt caaaaactga aattacacga tctgatgtag ttgcctttag
    13261 cccttgccat tcagcaatct tattacctaa ccaagaagaa gcaaagccag ttgtgtcatt
    13321 tttatattct ataaccgtcg catcagttaa tttcttctct ttttttaggg aatctaatgc
    13381 atcttcagag taaccaattt gatccaccaa cccattttct actgcttggt taccatcata
    13441 aattcttcca tccgcaagtt ctttcactgc attagtatcc atatttcttc cttttgctac
    13501 aacattgaca aaacgatcat aagcactcat cacaaattcc tgtataactt tattctcttc
    13561 ctctgaaggt ttgtgttgag gtgacatcat atcttttaat gcaccacttt tatacgtcgc
    13621 atcagttacg ccaagttttt ctaataagcc agaataattt aaaccagaaa taatgacacc
    13681 aattgatcca gttgttgttt cttccgtagc aaatatttta tctgaaccag cagaaatgta
    13741 gtaccctcca cttgccgctg tatttttaaa tgcagtatag ataggaatat ctagcttttt
    13801 aatttttgcc atttctttag caatctcagc actttcatat attcctcccc caggtgaatt
    13861 tacttctaat aacactccct taaccgcttt gtcttcttga attttcttta gctgtgttaa
    13921 aaaattctga tggttatatt gttccctact aaaaagattg ctttcacctg tatctgcaat
    13981 aactccattg acggatagtt tgacaatctt tttatttgat gcgccttctt ctaacgtttc
    14041 ttctgtgatt tcgttagatc cgtaaaaaat cttatttatt cctgttaggg ttgtatttga
    14101 tgcttcctct ttatcttttc ctggaattag cgcgacgata atggatagta acaataatcc
    14161 gcacgctact cccacagcaa tccatcttct tttattcata taaactcctc ctatattctt
    14221 agtaaaaata attatttttt atttagaaac acatcgacaa tttgttgcca atatgttttt
    14281 tctcctacaa ttaaagacaa ctctccagtt aatccataac gaaacggaaa cgattgagtt
    14341 gtttttaacg ttcctttcac tgcataaaaa acacctttct cagtggcagt agaattttct
    14401 gaaatttcat ctaaagttcc gtcgatcgta atcggagccc cacctttttg atccaattta
    14461 aaatgaacct tcatcccttc tttaatatag gtactttcat tcgcaggtaa aagtgctgta
    14521 aatttcattt ttgatttttc gtggatcgga taaatttctg caagcactgt tcctttggct
    14581 agttctgctt gtccctttgt ttcttcattt aaatgaaccg ttccagatac aggagctttt
    14641 agtattcctt ttgaaagttg atcgtccaaa cttttgataa ctgactggtt cttctcttgt
    14701 ttttcattta gctctgtaac tgtttgtttt acagttgtta ttgcttgttc tagctcttgc
    14761 tttccttttt ccgtttgact aattctgtca ttctcagaag tagttggttt aactaattta
    14821 gctttttctg actgatactg ttctacttct ttttttagtt gctcaatttg ttcatttatg
    14881 cttgctgaaa ttgtcagttt cacttgattt ttttgatcat cagaaacatt atttagttgt
    14941 tcttgccaat tctcatagtt tgccatcatt tcttttgaaa aatcttttaa tgattgatta
    15001 ttactccaag ctacctgtac ttgttgccat tcttgtaatt tactatctga actctcaatt
    15061 tgtttttcta gttgtctttt tgtcttttcg tatacttcta attgcttttg atcatttaat
    15121 tcactttgct tcaaagcgta gcggagtgat tctttttctg acaacatgct ctttaattga
    15181 ttactgtagc caaaactgtc atccgttgaa aacaaatttt ttcctttact tatactttca
    15241 ataaatagct gagccatctt tatttgttga gtaatacgtt ggttttcttg ttctatctga
    15301 gccttttcat tctctattaa agaacaatcc aatctaacca acacatcgtc cttatgaaca
    15361 aattgatttt cttttaggaa gttttccatt attttagctt ctaatggcac ttgtaacttc
    15421 tctatttttt cggggttcaa ttgagccgtt gtccgaataa caacctcttt tttagcaaag
    15481 gttaaaaaca atccaataat acataaaaaa agaattacag ggtataacac ccaacggtag
    15541 aagctatgat gttgtttagt atagatacta gaatgttcga accaattatt attctgcata
    15601 aattgtttcc ctctcctaga tttcccataa tttttgatag ataccatcgg caaaacgaag
    15661 ttcatcgtgc gttccttttt ctaccaaatg cccttcatgg agaactaaaa tttgatcaca
    15721 tgcttttgct attggtaaat gatgcgcaat aaaaataatc gttttttctt ttaaattgag
    15781 gagataattc ataattgcat gttctaacaa ggtatctaat ccactagttg cttcatctaa
    15841 aattaatatg tccggatttt ttaataaagc ccgtgctaag gctaatcgtt gtcgttgctc
    15901 tcctgaaaga ttatttccac cttcttccaa caatgtctcg taacgtaatg gctgttgatt
    15961 aataaattca tttagttgga ctatctcaca aacttctatt atttgttcga aacttggtgg
    16021 gctatctaaa ccgaatataa gattctcaat gattgttcca ctaaagaaaa atgactcttg
    16081 aggcaaatat gtcactcgat cgcgtaatat ctgaaaagga atgtctaagt gattaatgtt
    16141 tccataatgg atcgatcctt ctgatgcttc ataaaaacga acaaataact ttgcaagagt
    16201 tgattttcca gaaccactaa ctccaactat agccgtttta ctgtgcgaag gaattgtaca
    16261 tgaaatttcg ttcaacgttg gggccttcat gctatacgag aatgaaacgt tttttacagt
    16321 gatatcttgt tgaaataatt gttcagaaag catatattca aaagactggt tcgatttttc
    16381 tgaatcaatc gcgaaaattt catttaaacg attatttgca acttgagccg tttgcatttt
    16441 cacttgtaaa ttaataatgt tttgtaaagg atctgtcaaa aatactagta aagcattgta
    16501 tgtgataagt tgacctagac taattctatt atccataaca aaataagaac ctagccataa
    16561 aattaatgca ctgctaatta attgaatcaa atgtttaatt ccttgttgaa tattatcaag
    16621 cgtaaccgta cgaaatgatt ttttcattaa tttaacaaat tcatggtcta tacggttata
    16681 tactttctct tcaccgctat atgctttaat tgtttctatt cctttaagac tttctatgat
    16741 actagaattt acagctgcac cggctgccat ttcttccctg ttcgattttt catagctttt
    16801 cacgaaagcg aatattgcaa ctacataaaa aggcaaagag catagagtga ttaaaaacaa
    16861 cgtactattc tgaatagcta acgtaacccc aacaagcaac accataccaa tatctaaaaa
    16921 tattgataac gtagcacttg ctaaagcatc aataatctta ctggcatcaa gaaaacgaga
    16981 aataatttcc cctgattttc tcgtagcaaa aaaattcatt ggtaaagtta acacatgttt
    17041 gaaatactga agcataattg ccatactcat tcgctgtcct aaaataataa gaagataact
    17101 acgactatat tcaaacaata cccgaaataa atagacagcg atcaagccaa tggaaattat
    17161 atttaaagtt gaacgtgctt gattagggat gaaaaaatca agaatacctt gaaaataata
    17221 tgagcttcct atgccaaaaa acgtaatgaa aatcgatgct aaaacgatgt ggaatactaa
    17281 tcctttttgt ttccaaatta taggcaaaaa agaacttaat ccagctactt tttctttaat
    17341 aggaatataa gtttctctag gcataggtaa taaaagaata cctgtccatt ctttttgaaa
    17401 cgcttcaatc gtttttctat tttttccatt agcgggatct gcaatccata gtacgtttcc
    17461 cttcgtttta taaattacaa cgtagtgcat atagctgtta tcaaccaata tatgtgcaat
    17521 taatggtaag ataagatctc tttctttcca gatctcacta tctgcttgaa tagccataca
    17581 atcaaaagac aatttttcaa atgtcttttt caatccaaat gctgaagtac cctctaaatc
    17641 agtcccagat aggtcccgta atttatgaat cgggatatca gttttaaaat attttaaaat
    17701 catcgaaagg caagcaacac cacaatcttt ttcatcttgt tgctgcacaa aaggaaatga
    17761 ttttatcact tatctaacac aactccttct aaaaaatttt tatagaaaaa ttctcaatat
    17821 agctttaata cagtgaaaat attgtctcat aattccacaa aaactttgaa aacagttctt
    17881 gaacactaac tttttcattc ctgaatcaaa tttattttgg aaattttact attcaagaat
    17941 aaacttgata agtttcagga agatttttac tgttaatcaa atacgtatgt tacagtagca
    18001 ccgtaaaaaa tattatttaa agaagcgtgt ttaaacatgc aaaatgtaaa ggaactaagt
    18061 attgtggaaa tgcacaaact gttggtggag gcaataatgc ttggggaaaa ttggggcaag
    18121 ttgtaggtgg acttaccaca ggggcagtag gtggtgcagg tttaggaaca gcaatttgcg
    18181 gaccagcttg cggtgttgtt ggagggttat atggagctgt cgctggaggt gctgcagctg
    18241 gctgggatgc tcgaaaaaaa taaaaaagga gaataagtag tatgcatatt aaaaacacaa
    18301 aaacaacatt tatattatct tcagaagagt tgaaaaatat tcaaggtggg agtgccgtgg
    18361 gagttttagg cactactttt tctggggcaa cagcaggagt aaaactttgt tcagccggag
    18421 gaccttatgc aatagctgct tgtggtgtag gtggagcttt attaggtgct ggattcagca
    18481 tgtggaccgg tgcgtaaaca attaaaaaat atcaaacctc aagattggtt tgtgtcgatt
    18541 acgaacatag gtatgttctt tatcttttca aaagtttgga atggacagaa ctttttacta
    18601 aagcttcttg tgctagtttt tataattata ttaaattttt atcttttatt aaagtcagat
    18661 aaaaattctt tggaaatgta atggtagaga tatttttaca aaaggacttg cagcgctgta
    18721 gttggtgctg caggaggacc tatttcggcc gttacaggag gtattggtaa tgtacttgtc
    18781 ggatgtgcat taaagcctgt tcctgcaagt taaatttata aaaacaggaa agtaattatc
    18841 tcaaatccta tcaagtacaa tcataggatt gcacttgaca ggacaaattt tttaactaaa
    18901 aaatgaaaaa cactttctaa aaattagtag aatttgccaa atctttcgca tcttccacca
    18961 tacatttaat tttaattccc ctttatggta cttaataaaa acaagaaaag aactattcat
    19021 ttggcccttt tttggttcca gaccatagaa atattcctcc agtaactaat attgctgtta
    19081 tgcacgtaac taataacagc tataaaaatt ggtacaactt taataggatt cagaaaaatt
    19141 tgcactagtg aaggaatcta ttttattaga aaaatgcaat ctccctaaag aaaaaaccac
    19201 taaacagtgc tttctttcaa cttctcctta attttttgtg ctttccctaa agtaaagttt
    19261 ctggccaact ctgaatcaaa atatagtgaa cgttttttaa aatctatttc tttaacgtta
    19321 aagatattta ctaaacacgc tcgactaatt cttgttaaca taggatattt cttttcaagc
    19381 tcactaattc ttccataaaa ttcgtactgc ccatctttag tacacaatga taatctatgt
    19441 ggtaattttg aagcttctac aaaataaatg tcatttatat caaaagtaaa tgtttgagaa
    19501 cctatggaaa aaacaaatgc ttgattcgta acctgttttg ttgcatctat cctttcttgc
    19561 gctaataaca ataactcaac aatctcgttt cgatactcat ctagtgtttg atcttttgtt
    19621 acaaatccta acgtttcaac tcttcttttt attgttacgg gtaacatctc atcatgagta
    19681 gttgtaaaaa tgatttttgc ttgaacatca tattttctga ttacttctgc taattctata
    19741 ccgttaactt catgatttaa atcaatatct aagaaatata ttccttgctt tggtcggaat
    19801 tgtttgaggt attttttaac ttctaacgga ctttgtgttt tcaatactat cttaaatact
    19861 ttatcgtgaa aaagaatgaa attatcaata attcgttcta actgatttaa ttgtataatt
    19921 tgatcttcac ataatattat tggataactc ataaatcctc cttccctcaa aacaacaaaa
    19981 taacttgagt agtaaattga tacggatcgt tttgatagtt cacaaacata ttgggattct
    20041 ttttattgat ttcctctata ctagataaac ctaacccttg atgtcccttt tttgttgtta
    20101 ttcctttttt cattagagta tctatagaga gtagtatctc tctacaacta ttcgatatcg
    20161 aaatttctat ctgtttatca tctcggtaaa tagctagaga cagttctcta tcttgactct
    20221 ctatagatgc ttcaattgca ttatcgagaa taattccaag caccctaata cagtcaaata
    20281 ttggaacagg aacttcaata atagtacttg gacattcgaa tttacaacaa atcttattgt
    20341 tatttgcttg gtatagcttc gctatcaaca aacttttcaa atattcattt tttatatttt
    20401 ttaaataacg atattcaaac ttattggtta gataagaatt agaatagatc tctaattctt
    20461 ttatttcttt ttccaatatc gaatcatggt tttgattagc gatttctttt aaactcagca
    20521 ataaattttt atagtcatgt ctaaatctgg caaatttttc ttgatctttt tccagtgaat
    20581 cagtatattt ttttagataa accaattctt ttcttttcaa ttgctgttca tattgttctc
    20641 gttgcctaat agttattcta ataaataaaa atagaacaac aaacatttga atgattaaaa
    20701 atattaagac accaaacaca aaatgatcaa atacttgata ttgatgagca acataagaaa
    20761 caaataaacc aattgtaagt aaataaatca tgaatatagc agtaagttta gaactggact
    20821 gttcaacaat cctatttata gctaattttc tatataacca aataaacatc agcaaaaaca
    20881 aagaatcaag cattatctgg aatactacat aaccataccc ttttatccct tcaaatgaaa
    20941 acatatacgt cataaccatg gaagtaataa tagatctgaa atcatcaata agcatacata
    21001 atagtaattt atttaaaagc actatattag ttttcttctt ttcctttaat ataaaataac
    21061 ttccgattat aggtatagct gcggaaacat cagtaaataa ttcaacaaaa aaagtgagtg
    21121 gtatcaataa acatacaaaa agatattttc ttttgttaag atcagtatta gtcatataaa
    21181 ataagagaaa aaagaaaaag tttcccacga acattcttaa tagataagct tttgtatcga
    21241 tagtaatcaa gagatcccct cctgatttta ggatatcata aaaaaaattt tagttacact
    21301 ttttaaataa tgaaaaaaca cattccacta aatttgatgc cggttttcct tgaggttttg
    21361 ttccagcacc tcctttaatt tttttaagct cttgatctga acattgttta gcatttagtc
    21421 tatttttttc ttccattacg attcccccta caaactat
//
LOCUS       DAJSAW010000046        21216 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJSAW010000046 DAJSAW010000000
VERSION     DAJSAW010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.046"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(59..775)
                     /locus_tag="ONT64_001945"
     CDS             complement(59..775)
                     /locus_tag="ONT64_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HCR2303355.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(1002..1544)
                     /locus_tag="ONT64_001946"
     CDS             complement(1002..1544)
                     /locus_tag="ONT64_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR2303356.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(1549..1776)
                     /locus_tag="ONT64_001947"
     CDS             complement(1549..1776)
                     /locus_tag="ONT64_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR2303357.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(1855..3735)
                     /locus_tag="ONT64_001948"
     CDS             complement(1855..3735)
                     /locus_tag="ONT64_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCR2303358.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(3924..4538)
                     /locus_tag="ONT64_001949"
     CDS             complement(3924..4538)
                     /locus_tag="ONT64_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2303359.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            4680..5240
                     /locus_tag="ONT64_001950"
     CDS             4680..5240
                     /locus_tag="ONT64_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2303360.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(5545..5859)
                     /locus_tag="ONT64_001951"
                     /pseudo
     CDS             complement(5545..5859)
                     /locus_tag="ONT64_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(6113..7748)
                     /locus_tag="ONT64_001952"
                     /pseudo
     CDS             complement(6113..7748)
                     /locus_tag="ONT64_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8303..8866)
                     /locus_tag="ONT64_001953"
     CDS             complement(8303..8866)
                     /locus_tag="ONT64_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR2303361.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(8881..9924)
                     /locus_tag="ONT64_001954"
     CDS             complement(8881..9924)
                     /locus_tag="ONT64_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303362.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(10057..10461)
                     /locus_tag="ONT64_001955"
     CDS             complement(10057..10461)
                     /locus_tag="ONT64_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR2303363.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(10694..11329)
                     /locus_tag="ONT64_001956"
     CDS             complement(10694..11329)
                     /locus_tag="ONT64_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCR2303364.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(11595..12647)
                     /locus_tag="ONT64_001957"
     CDS             complement(11595..12647)
                     /locus_tag="ONT64_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2303365.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(12660..13598)
                     /locus_tag="ONT64_001958"
     CDS             complement(12660..13598)
                     /locus_tag="ONT64_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR2303366.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(14049..14699)
                     /locus_tag="ONT64_001959"
     CDS             complement(14049..14699)
                     /locus_tag="ONT64_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303367.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(14799..15308)
                     /locus_tag="ONT64_001960"
     CDS             complement(14799..15308)
                     /locus_tag="ONT64_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR2303368.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(15509..16378)
                     /locus_tag="ONT64_001961"
     CDS             complement(15509..16378)
                     /locus_tag="ONT64_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2303369.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(16398..17519)
                     /locus_tag="ONT64_001962"
     CDS             complement(16398..17519)
                     /locus_tag="ONT64_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HCR2303370.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(17521..17916)
                     /locus_tag="ONT64_001963"
     CDS             complement(17521..17916)
                     /locus_tag="ONT64_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR2303371.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(17935..18741)
                     /locus_tag="ONT64_001964"
     CDS             complement(17935..18741)
                     /locus_tag="ONT64_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2303372.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(18734..19522)
                     /locus_tag="ONT64_001965"
     CDS             complement(18734..19522)
                     /locus_tag="ONT64_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2303373.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(19536..20012)
                     /locus_tag="ONT64_001966"
     CDS             complement(19536..20012)
                     /locus_tag="ONT64_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2303374.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(20084..>21216)
                     /locus_tag="ONT64_001967"
     CDS             complement(20084..>21216)
                     /locus_tag="ONT64_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2303375.1"
                     /translation="KIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANR
                     IVERYIYDAIDMPLMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDA
                     QKKFGLLNNVSTRMAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCS
                     CASGLGTSFKLKKILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLN
                     PHLEDVEFFSIEDMILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTI
                     LNPTKLLERVSDAIYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNE
                     TSEEFQKFQTHFKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         6580 a         4350 c         3232 g         7054 t
ORIGIN      
        1 aaataaactg agcaaggggt tgtgataaaa gacatgtcac aaccccttgt ttatctcatt
       61 aatttgcttg tccataaacg atttgattaa agccattttt caattcatta ttcgctgtct
      121 cgttgaagta aagataatta tcgggattcc cctgattttg ttgtgtcacc aatacttttg
      181 gctgattttg ctgaaacccg aatagataga cgtgcttttt cagatatggt tctgtctctg
      241 catcggaata gacggctgtg atttgatagt ctgcttgacc tgtcccactt tctgaccatt
      301 ctgcagaaac aggcactcca ccaatagcca tcttccactc cccgttcaaa atagcttgag
      361 ggactttaag accatataag tccacactca tttgattcgt ataacttttg tattgttgtc
      421 ccattgtttg gccccatttg gtcataaatg cctctaattg tgcggatttg tctgcattcc
      481 acagtgtttg ttcaacttgt tcgctgcttt ctgaagagga agatgaagcc tctacagaag
      541 aacttgaagt agatgtttga gttgtttcag tcgttttgga agactctgac gaagtagagg
      601 tatctgctgt ttctgtactt gctgcagttg acttgctctc tgtgctagtt gttgtagtag
      661 ttgcaactgt tgctttcgct gtttcttttg ttgattcttt tttcgattca ttcccgttgg
      721 tacaaccagc taacaaaaaa gctagaccaa ttgttactaa aatccttttt ttcaattaaa
      781 aaactccttt cgattactcc cacttcttta ttctatcaaa taagcaatgt gattttattg
      841 atttttcgta ttttctcatc aatctttcaa atcttctaaa aactgagaag cacccgctga
      901 tgttgtctgt aatacatcag cgggtgcttc ttttatttta atcttcgtga ttttccaatt
      961 tacctcacgc tttatatttg tatttgaaga aacatagact tttagtcatc atcgtcttcc
     1021 tcatccaaat cttgtcggac agtttttcct tggagtcttt gtacttggta gatgatttct
     1081 ttattgaaac ttaaaagttc aacaagcaat tgttgcaagc catatttttc ttctttttca
     1141 gctaatttga ttgcccgtgt gataaagaca ttttggaaat caaaatcttt tgcaaagttt
     1201 tccaacattg ttgaggctgg taaatatttg ttttgaccat gttcgtaaat ttttccataa
     1261 cgcatgactt cttctaaagt agacggaacg atttcatttt caatcactaa ctcatgagag
     1321 attttattga accatctcgc ttcttgatca gcatattctg aaaaaagggt acttaattct
     1381 tgcgaagatg tacccaaaac atacaattgt gcttggatca atttcatttc ttctaattta
     1441 aaatttgcta atacatgccc caccatggcg ccagcagttg gtttatgatg gtcgatatct
     1501 gcttgttttg tttcttgaac aaatttttct tccactatat tcattgattt acgcctcttt
     1561 tacttttgct gattggactt cataacctaa tttttccaca actgcagcta attgttcagg
     1621 gtggacttca tctggatcaa agtttgcttt gattttgctc gcattaaaca aaactttcgt
     1681 attgcttacc ccaggctgtt gagaaaccgc atgttcgatt ttttgcatac agcttggaca
     1741 agctagtgta tctaattgca taataatttt tttcatcgta gttcttcctt cctttttctt
     1801 gatagtctta ttctagatta cagcttccat aaaaaaattg attcttatca agttttacga
     1861 tttttcttct ttgctttcgt gtccaaaata caataaacgc atcgcgttaa agataacgat
     1921 caagatacta gcttcgtgaa cgaacatccc actagccatg taaataaacc cggcaaataa
     1981 gccgttgagt aaaaataaga cgacaccaat ggcaatcact acattttcac gggtatttaa
     2041 aacagttttc ttagccaagc ggtaggcatg aaccaacgat tcaaaattag attgcatcaa
     2101 gacaacgtca ctcgtttcga tagcaacatc cgttcctgat cccatagcaa tcccgatatc
     2161 agccgcagcc aaagagggag aatcattgat tccatctcca ataaaagcaa cgcgcaatcc
     2221 ttgttcttgg tattttttca caaattgaac cttttcgtct ggtaataatt cagcatggac
     2281 ttcatcaatg cctaacatat cagctacata atcagcagtc atctgattat cacctgtcag
     2341 catcactaac tgttttgctc ctgcttgttt caacttagct aattgttcag cgacttctgg
     2401 tcgaatcaca tctgacacac ctatgatttg agtgattttt ttgtcgattg ctacaataat
     2461 gaccgagctg cctgtttgtt gtaattgatg taaatcttgt acttgctttg gtgtgagaga
     2521 aagtttgtga gcggcaatca atttggtgtt gcccgcacaa actgtatgac catcaatctc
     2581 agctgtgatt ccttgtccct tgattgtctg attatctttg actgctaatt gttgaaaatc
     2641 aatgtctttc tcctgagcat aatggatcac ggcgcgcccc agaggatgat cggataacgt
     2701 ttctaatcga gccactaaag ctaagctgtc cccttcatcc tctgtatagt tcttgatagt
     2761 ggatacagca gtagttcctt tggtcaatgt accagtttta tcaaagacaa aagtatcgat
     2821 tttggaaaaa gtatccattg cttcaccgcc tttgactaag atgccccgtt tcgctccatt
     2881 accgattcct gctacattcg aaacaggcgc accaatgact aaagcgccag gacaaccaag
     2941 aaccaaaaca gtgattgcta aacgaaaatc acgactaatc agaccaacaa ccaacgcaat
     3001 aagtaaaacg gttggagtat aatactgaga aaatcgatca atgaattttt ccgcatggga
     3061 tttggtatct tgtgcttctt ctactaattc gataattttg gcaaaggtcg tatcatctcc
     3121 gaccttagtt gcttcaatcg tcacgtagcc atctgctaaa atcgttccag agaagacttc
     3181 atctccactg attttgacga cttgtcgtga ttcaccagtc actgcggctt catccgcata
     3241 ccctttcccg tcaatgactc gtccgtccac aggaatagct ccaccagttt ttactaagac
     3301 gatatcgcct tcttccacgt catcgatttc cacttcttca gtcgttccat cttcttggac
     3361 aacactagca gtagatgggg ccatctcggt caaagccttg attgactccc gcgttttggt
     3421 caatgttttt tgttccagat agctaccaaa caaaaacaag aatgtaacga tagctgactc
     3481 cttgtattct ccgatcataa aggcaccaag cacagcaata gtaacgagta aatcaattga
     3541 gataactttg actttcactg cctgatacgc atggatcata ataggtatga ccgcaataat
     3601 agaagctatg gctagagcaa tgttatagaa caagacaaaa ccagtaaatt ttccgataaa
     3661 accaaaaata atcaacacag ctgataaggc ggtgatatga tttcgatgct cttggatcca
     3721 cttttgtaat ttcatctttc tccctctttt cctttcattc ttacgagact aagtatagat
     3781 acttatccat cataaaaaat tgaccactat caagtttagt caaaaaggcg gttttctttt
     3841 ggtagaatac actacaaaaa atagttagca agaagactca ctaaaagtga tcttgccaac
     3901 tattgtcgtt tctatcgaaa ttttcattga atgattttcc tgtacagcat acatcggaga
     3961 tctatttctt tatctaagat ttttatcttg ctaatttttt cctttagtct ttcaaatcca
     4021 cgtcgttcat agaattgata cgtacagcca ctatctgtaa aaaggaatat ttcttttcct
     4081 ttctctcttt tttcaaattc tgttagcaac ttggtaccga tgccttttgt tttcaaatca
     4141 ggatttgcag ctaaaaaaat gatctctcca tctggcgaat gcttctttaa atatgctgca
     4201 agcatttgtt catttgctgt ctcgtaagct cctttacttt ctttagcaaa tatattttgt
     4261 aaaaaatcga atatcttaac atatatttgc tgtccaaaag aacgatgttt ctttttttct
     4321 ccttttatct ccgccagcaa aacacctgcc aactcgtcct ctgcatacaa agcaagaatt
     4381 tgagttgctc gattcaactc catatacaaa aaatatcttc cgtaaagatt cagcagaagt
     4441 tcattttcca tatatctctc aaagttcatt ccttcaacag caaaatagat agcttttttg
     4501 taatctttct tttctaaggg tttaaaagtt agtttcatca tttcgcctcc tcaacttcaa
     4561 tgattgtgtt atacttacat tataatagac acagtgtcta ctatctataa tcaatcatct
     4621 ataagtttgt cgattaagcg acagctttgc tttgatatgt cggataagga gataggtaaa
     4681 tgaaaaagac agatagacgc gtcaaaaaaa cagaaaaagc tttggctgaa acactatcca
     4741 cattattagt aaataaaaag attcaagcaa tcactatacg cgaactaaca gaaacggcag
     4801 atgtacatcg ttcgactttt tatacacatt ataaggatat ctatgatcta tatgatcaat
     4861 tagaaagcaa cttttttcgg gatctaaatg aaattctctc ttatgatcca gcccatagct
     4921 atgaggaact atacactcgc ttgattgact atgtttacgc aaatactttt ttatatcgct
     4981 tatttatcct caaccatggt gatatttcgt ttcagaagaa agttaaacaa gttattgaag
     5041 aaaactatct gaacatatgg ctatatgaag acaagaaaac gattatcaca gaagaaatga
     5101 gattctttac tacgtaccac gtccaaggtt gtctatccgt tctcaatcgg tgggtagaaa
     5161 gcaattacaa gtactccaaa aaagaattat tagaactctt acagaaactg aatgatcaca
     5221 tagagaaaat catgccttga ataaaaacat actaagctta gttaagaaga accgtaacct
     5281 tctcgtataa caactgaatc ttatttagcc aaattgttta ctgaagattt tttgagtaaa
     5341 atttctataa ttcctactta ttgaaaagta gtttgtagtg ctgcttctcc ctctctaagt
     5401 gatatatctt gttgtaccaa tcaaattcac ctcatcatta ataaattact tttaggctaa
     5461 tttattacaa cttataggtt gaagctgtaa ccaatttata gtcatattat atttttatca
     5521 tccagtttat tgaattcttc tcgtttacct caattcattt tcaaggggaa tcagctcatc
     5581 atctaacatt ttacgccatg ctcgcttcat ttcttgtggg tatttaaatg gtttcgcttc
     5641 tggatgtttg gctttatatc cttcgtaatc cttactgttg ataattgctt tcatattagg
     5701 aattggatct tcacctggca tatattgcag attagaagca tgtaattttt tctcagaaac
     5761 aaaccactca ttggaaaaga catgaatcaa atcatcactt acttgtgtaa aatagcgacg
     5821 tttaatgaca aagatatctt cattgctatc tagtgtacct tcatttcttt gtgcttaaat
     5881 tcaaagataa ttttctcaat aaagattttt gattccagta agattccttt actcctttta
     5941 caacatagcg ttgtcctttg ttttgaaaca tcttcttccc ctctttgcca ttaaaaatag
     6001 cccaattctc tataaagaag attaagcttc attattataa tataaagttt aaatttggtt
     6061 tattacttac tttttcttat tctctagtaa gctaatattg aaaaaaatgt aatcagatca
     6121 ttatttttat atgttgtaac ttcctgataa gaaaactaat taaaagaaag aggtaactac
     6181 ataaactaat taaaaaagct aacttcatag ttggaggata aatggaagaa acttcaatga
     6241 ttgtttctcc ttgtgttcct tcaatcgaca ctagtcctat tatcaatctc tcttttttgt
     6301 ccatttactt ttgcgactgt tccccaataa tcaaatacag gaagcgttat tttttgtcct
     6361 ttagattcat gtactgtata gactattttc gaatccatca cttgataagg catagtatgc
     6421 cattccttgc caattcttac ttcatgattc tgcaaatgtt ctttgtattg taatgcttcc
     6481 gtgggtgcat agtcataaag tcctccggga aggagtgtcg tgtaacgaat aagattttct
     6541 tgattcatta ccaagtgatc tctctgagtg acaactttat gttcatagtt tgcttttgtt
     6601 gcagcagata cagccaacag taataaaaca attgccgtta tattaactac ttttctttgc
     6661 ttagctgatg tgctcttcat gaaaacgaac gataaaatca acgagccaaa cagtacttga
     6721 aagcctaaga ttcgccaagg aaattgaatc atttgaatgg gtgtattttg taataaagac
     6781 catggaaaaa atttggtgga taagagccaa gagactaccg cactaaggta aatcactttt
     6841 tctttgcgat taaatccttt aaaaaagaag agtggacaga tcaaagaaat aataccaatg
     6901 attcctaccg taaaggttgt caggtcatta ttcatcgcac caatcaaact atccgagaca
     6961 tttaatgctt ggtgttgtaa ggtcgtttta tctggctcat taatcgattg aaccatgatt
     7021 tgtcggaaca tcggatacca aaagtatgct gttaaaagaa tcgtcattcc tactgccttt
     7081 gacaactcca caatcaaccg gatgctttct ttttttcttg tttcacttac tacaaagccg
     7141 cctaagacta gtaagccaac ggctattaca ctcataaaag tggaaagaac atgtgtataa
     7201 atgagtaagc tcatactgac tgctaatgga attgcacatg gctttttctc ataaatcagt
     7261 gcgtaagcat aatacataac tatcggtata accgtaagtg caatcgcttc agcaatagca
     7321 gaacgatgat aaatatcaat cgtacggtac ccataaaatg tatataaaca gctaaatata
     7381 aaagccgcat cgattctttt taaaaatttt agtccataat gaaacatcag catgatagta
     7441 caaacattca atagccaaac atataagata taactaacga ttaaattatt acttatccaa
     7501 taaaaaatga ctgccggaaa aaaggtcaag tagggataaa aataattcac tccgctccca
     7561 taatgattaa acgtcgtaaa attgattgga ccagaaaaaa ttgaagataa ccctttgact
     7621 ctagcaaaat ggaaggcaat atcccccgat gtagaaatag tatgaacatt tctaaaataa
     7681 ttgattgaat aaagtccagc aataattgct aggagaatgg acaatattat ttttttcttt
     7741 acttgcatta cttaattata tactcctttc atggattgct ttatctagta taacttaaat
     7801 tttaaataaa tcgttattta tttttactct cacttaaaaa agcactaaaa ttataagaga
     7861 gggaggtaat ggtctgaaca ccaagaattg ttccaacatc gaattgctta actaatgagt
     7921 tcaaagctta attatgtggc tcttatattt accgagcgtg gattgcgatc tctttagcga
     7981 aacaaacatc tttcataaaa aaaacagcca tatccgcttt cttggatatg actatttact
     8041 actactttac tatctttcat tcttcttgtc tcacttatga taaggcgctg cgtatcatga
     8101 gtgaatgata ttaggaaaac ttcataaaat aaatctttta tacatttcca cttctagatg
     8161 gtcattcatg ttagaagaat caaactattt ttttactgtc gtaataatat taaaacacta
     8221 gaacaaatca attaaattcc ataaaatata aataatagac ctagcatcat ctggtataaa
     8281 atggatgcta ggtccgttat atttactcaa aattgattct acgaatagat cttcttatca
     8341 tttctatttt agtagtcatg cgttgttgtt cttctaaagg tggtaatggt ataagtagtt
     8401 tagataaatt tgtaccattt acatttggtt gcccagttcc gtaagaaatt ttttctaata
     8461 acttccaata taatggcgag tctaagaagc agtctacgta ttctgataat aatttctcaa
     8521 ctaacctaat tcgaatcaag taagaagcaa aaacagattc ctcacttatt tccttaacca
     8581 agaaactttt tcccatagtt cctcctgtcc ttgctatcaa aatgtcattt tcttccaatc
     8641 ttaaatcaac tagtttagaa ttactaatat cacagtaagg cacactggac caatttacac
     8701 gtccctcttg tatatctgtg attctcaaaa acttaacatt gccttgtttt ttcgccgaat
     8761 ctgtatagcc atattgaatc ttagtagaaa tacttcctaa ataaacccat agtcgaacat
     8821 cgctagatga tttttcttgt cgttctttat gaacaacagt gatcatcttg agttgtccga
     8881 ctatttttta gttgcagcaa tctttttggg acgcttattt gcatgaagca acgttgctaa
     8941 gtctagtaat aaaacattgg ctaacacagg attggctttc tcgcgatacg ctgcggcgcc
     9001 actttcaaac aatactgcta gacgatcttc tacaacaacg atttgttcca aatacggtgg
     9061 aaatttgatt cgttttagga tccggctagg agagtcccgc tcggacacta agagttccga
     9121 ggtataagga ccataagaac gactggctaa gagataatgg ccataaaaag ccacaccttg
     9181 aatcttatca gatgtttttc tgctcattgt cacggttggt tggattttgt ctttgcggcg
     9241 aattgctgtt ttcatttcag ctatcgtcgg caatagatag aaagtcaact gtccttcacc
     9301 ttttagtgtg aagttgccag ataccagttg atcattgttt tgagtcaaat acgatgcttg
     9361 tggaatctct gagagatcag ttgtcagcca ataagcgatt ggctgtcccg tctcttcaga
     9421 tgtatctgct aagatatcga tcaaacgtaa agcggctact cgtccagtag cagatcctgt
     9481 gattgtcaac cataaaagtt cccgttttgt atcatatact aagcccccta catgtggccg
     9541 tttaggtaaa atcagcagtt tgatcggctg tcctgtttct ctatccagta catgaataat
     9601 agaatgatgc tgatggtcat agcagtaggc agaaataaac acaaaatctt gagaaaaagc
     9661 caacccttgc ggcgtcatcc attgacaaac ctcattttcg ccggtttccg gcaccaccat
     9721 tcttgtttct gttaatccag gaatgttata ttgttttgct tcagcctttt cccaagctgc
     9781 ttgaacaata ggataccgtt ttaacttttt ttcaatatcc tgatgtatcc gttgctgctt
     9841 ccaatgctta aactttagga catagtaaac aagaaggcca atgaccagca acactaaaat
     9901 cattattgct acccgctgtg tcattttttt gcctccttgt tctattcgat caatcgtcca
     9961 tataccgcta ttaattaccg taactacatg tttcttttac tagcgtttct ttataggatc
    10021 tttcataatc tcatttatag taacattaag aatcccttat tctactatag tcacttccac
    10081 atcaatattg cctcttgttg ctttagagta tgggcaaaat gcgtgggctt cttctgcgat
    10141 tttctctgct tggtccttct caagtcctgg caaagaaact tctaaaacga ccccgatttg
    10201 gtagccccca tcttctaatt gaaataaaga tacccgtgct ttgacttcag aatcagactc
    10261 aagattattt tcttccagca tgtgttgaac cgcgccgttg aagcaagatg cgtatccagc
    10321 cgcaaaaagt tgttccggat ttgtcccttc actatggatt cctgggcggt cgatcttcat
    10381 gtgcatactt ccgtttggtg cctcaacttc gccatcacgg cctccacgat tgatcattgc
    10441 tgtttcgtac atttttttca ttgagatcat cctttctttc gtcgttctat attaagtgta
    10501 acgagtattt tttaatctga caaaggtaat gcttgcattc ggcgacatca gttagacaac
    10561 atcgatgatc agtgatagaa gcagtttatc tatacaaaaa aagaagggtt ctatgatatt
    10621 tggtgtcctt actcgccttg agtaacaaca ccaaatatca tagaaccact taatcagctt
    10681 cacactgctt tttctacttt cgtagttgaa aaaaacttaa acaaatcatc ggcaaatagt
    10741 taaagaaaaa acttaatcgt tgaaagaagc ccaattgctt aaagaaggca aactgactaa
    10801 tcgacggaat ccgtgctaat ccgtaaagac ctgcaattag taaactaata actgttaaaa
    10861 caagataaaa atggctaagc gtaccttgac cactttgacg ataaagcaga acaagtaaca
    10921 atgggaaaag tagaaagcct gcatagccta gcccagaacc tatattatgg atccaagtcg
    10981 atagattaca agagctttca ttcgtattga tactgaataa gccagtaaaa atacaatcac
    11041 ctatcccata taaaccaact gttgcaacaa caagtattgc aagtgttttt gatgttccaa
    11101 caaataggtg atacaacgca ggcaacgaaa gaacaaaaaa aagtccagat acaactgacc
    11161 agaccaagaa agcacgtctg actggactat ctacgtcacc aaacacacta attacggtag
    11221 tcatttgatt cagtttggga taaaaaaggc caaggatata cggtgtcgat aaatcactca
    11281 tcacacctaa taataaaaaa taaaaaccat atttttgcaa aaaaatcatt ttcttcccct
    11341 actttttagt gattattcga tacatccaac aatcaaattt tttctcattt taacacatat
    11401 ttttattcta gttgtctttt gaacaatcaa taacataaag gtgagtttct tctacatttt
    11461 aaaaaatcga atggcactga agagttcagt tgtcagcatc aatctcctac tcaaaaaggc
    11521 aaaaaaacgt aagtcagatg ttgttgacat ctcacttacg catccattga ttgaaaattt
    11581 cttagtaaaa tctactagcc taaaaatttg aagacttctt ctcgatcata cagtttaaat
    11641 actttctcgc ataaaaattg agtaattagt gccgcaaaaa cagggatgac taaccaacaa
    11701 agaacaacaa gaaagccatt tagccctgca tctaaagaag ccaacggacc aacaagtccg
    11761 accacgccga aaccagcaga agcaggcgta cctgaaatag aaaatagagc gacaggaata
    11821 gctgtaatca ctgcactaac taaacaagga ataagaatca caggttttct aaatagatta
    11881 ggcatcatca tcttcatagc gcctaaagca atcgcaattg tcacacctga cttattaacc
    11941 ttccaagagt tcactactaa aacaaaagtg gttgcagcta cgcccatcgc agccgcacca
    12001 gcagaaacac catctagttg gattgccatt ccgattgcta ctgttgaaat aggagagata
    12061 atcaaaaagg caaaggaaca accaatcaag atagacatta acagcggttg taaggtagtg
    12121 aagttattaa tcaatgatcc aattgcagaa gtaattgctg ttacaaaagg gaataaataa
    12181 aaaccaagta aacctgcacc tacacctaca acaatcggtg ttaacacaat cgacactgac
    12241 ccaaaactat ttccaatcag taaaatcagc ccgacagcaa tcgatgctgt cagcatcgta
    12301 ttaataatat cgcctgtacc agcaccaacg taaacacccg tttcacccaa atcaggtgtg
    12361 aatttgacta caccagaacc aacaaaagaa gctccggcta caaccatcat tctcatcgga
    12421 tcaaatccaa attgtaaggc aatcaaacca ccaatgatca aaggtgttgc tagttgaaaa
    12481 ataactgcta actgtgcgat cgttaccgca aagggatacg cactaaaata ttttaaaatg
    12541 gcacctaata ctgcattagg aatcaatcca ataatgattc ctgttgctgt accagcgagg
    12601 actttattca gaaaaatttt cggtgatatt ttttgtgaag tttccattca atctctcctt
    12661 tattttacgt tcaaactatc ttcttttgca tggacaagct cagtcaagaa atcacaataa
    12721 ggcgtcgcaa caccatattt cttccctttt ctagaaattg cgccattgat ataatcgatt
    12781 tctgttaatc gatggttctt aatcaagtct tgatacattg aaggataatg taacccaatt
    12841 gtttccgggt caaaacaaga ttcacaatgt gcaatgactt ctgggacatc tagttcaatc
    12901 ttctctactg ctgctacttt ggcaaactca ttgacaatcg tcgccaccat tttatgagca
    12961 gtcgatgttt ttcctagctc agccatatta acgtctaaga ttgtgcatag tccgttcatt
    13021 gttccgttca cacatgcttt gcgataaata gaatagtgaa tattgtcaga aaaatgagca
    13081 tttaatcctg attcagacaa tttgtctgct aatttttttg cagctgcttc ttttccatca
    13141 cctaaatttt gtaattctac tgaaccactt ccaaataatt tgacctgacc aggaccttca
    13201 agacctgccg tccacatggt atttccaata tagatatttt ccattggtac aaatttctca
    13261 ataatatctt catgcccgat accatttaat agacataaga cctctgtatc ttttttgatt
    13321 aatgattgga tatcctgcag cattttttcc agctgcatcg ctttggtaaa tagaataatc
    13381 agatcaactt gatcttcttt ttctacttcg gattgaagaa cgattggtag ttttgcttct
    13441 acctctttac cattaaaatt tgcttgtaat ccatgttctt tgatttgttg gacatgttct
    13501 gcccagccat cgattaataa tacttcgttc ccactttggt gaagcatcaa cccaaagcga
    13561 cttcccattg cgccagcgcc tgcaattgct attttcattg attcttcctc ctttttcttt
    13621 ctattatact catttgagaa tcttttcaca acctcgtttt gttattcttc tcttatttat
    13681 catgtttata tccaccattc aagttatttt atcagcaaat caataaaaac tggaatagaa
    13741 tcccaatttc atcgtaagag aaactagact agcaataaga aaatagaaac attcatagaa
    13801 aaaatctcac tcctctgatt ataaaagagg gggcattaac ctcgtgtata ctaaaacctt
    13861 ccattcattc gatggatgat tttctttttg tgaaaaaata ttcattgtag tacgatgttg
    13921 cgcaacacag acggatggta ttgcttatta tttattaaaa tcgaacaata cacttattta
    13981 aaaacttctt tcccagattt tcttccattt ttacagttat aactagtatt atcatgaaaa
    14041 atagcctttt attgtctatg ggaataataa tttaaaattg gtactttttt attaattagt
    14101 tcatcgccca ataatctctc cagtttctta gcatctcctg actcaaccgg aattatccaa
    14161 gcccagtcta aataattttt taaaatctca tcatattttg caaatatttc attgccatat
    14221 tccgtcctta aaattatatt ttcagtgcca gacaagtgat ttatattgag ttgataaaaa
    14281 agatctccat ctaaaaaaat atcattagaa gaagatttcc ccacagtaat gaccattcca
    14341 tcagctctat gtgttacata caaataattg tatgacctca tttcacctaa tgaatcaatt
    14401 atttcttgcc ctttagctct aatatattga tctctattac ctatctcact cttatttaac
    14461 aaaagttttt tcatgtcaaa tttaatcggc tgttttattt ttgtaaattc gcttctacta
    14521 ctaatccaat ccgagattaa tttttcaaca tgtcccagag taaactctga tttattcgct
    14581 aaatactttt tttgttgtat taaagacatc tttccccgaa gctcaaaatt gagcctacat
    14641 cgattctcta tctcttcaaa aagcatctta tcttcatcat tcttatattc atcattcata
    14701 aaaccaatcc agctcctcga ttaagataat attttaatct catacaaaaa ctagctctca
    14761 tcaaaacaac cattgttttg atttctcatg aaatttcttt atacttttct ccgattataa
    14821 attcctaacc ccttgagaaa attacgcata tatttatctc cgcaaggctt aaaatttcgt
    14881 tggccttctt ttctcaaaaa tgccccgatt tcgccttttg atggatagat ttctgcctca
    14941 gcaaaaacgt ctaaaatatc atcagtggtc atagacaagg cgatttttaa tttttttagc
    15001 aacacattgt tgatatattt gatttccttg cttttttgcg gcgtgtctac tatcggctta
    15061 tctttttgcg gaccacgcgc caagagaatc aggccattta ggaatgcgtc aaaaacttgg
    15121 ttgttgatgg ttttttcata cacattattt tctgcattat ctttgttctt atcctgtact
    15181 ttggtcagta ccgcctgagc ttcttcttta gtcagtggta caccgcctag ctcaaaagct
    15241 ttaatcaaat ccgtatcttt caaatcaatc gcataacgca atcgcaataa cctatcattg
    15301 ttattcattt gttcctccga agtatttagt ctttactttc ttacgtactt tttgtcttgc
    15361 taagattttt aggatcaatg ataaaaaccg catcacttat gataaggcgc tgggtatcat
    15421 gagtgaatga tattgctgaa tacaaatagt ccgaccacat taagttgatc ggacttcata
    15481 agcattttat tgactaatac ttttttgctt aagaattgat tctaacatct tttgctaaag
    15541 ttaagcatcc tatagttcct gcattatctg ctaacttagg tgtgacaatg tattcttcga
    15601 tatttggcgt atcgacatag ccgttaatta gttctaaaaa acgtctttgt actttctttt
    15661 tcagatgttc ttgttgcatg acaccaccac caaaaattat aatatctggt gaaagcatca
    15721 gggtcgtatt acaggcacat tgtgcaatat aatccgcctc gatttcccag aacggatctt
    15781 ctatagaaag atcttgtccc ttggttcccg tacgcatctc gatagcgggt ccagatgcca
    15841 acccttcgag acaatcatga tgaaaaggac aagtaccaga gaaagtatct cctgaatgtc
    15901 tcttaactaa cgtatgcccc atttctggat ggctaaaccc ttcaacaaaa atcccatctt
    15961 gtatagctcc tccaccaatt ccagttccaa cagtgtaata aacaacactg gataaacctt
    16021 ttccatgacc tgaaacatac tccccgtaac aagcagcatt cacatcggtt gtccaggcaa
    16081 ttggaatttc gaaatgctta ttcaacgttc caacaaaatc aaaatttttc caggcagttt
    16141 ttggagtaga ggtgatatgt ccataggtct ttgattcacg atggatatca atcggtccaa
    16201 atgaccctac tccaatgctt tctaattgtt ctttatagtg attaaagaaa tcaattacca
    16261 aactcattgt ttcattcggt gtagttgtag gaaaactcac tctttctaaa acctttaaat
    16321 catcattgcc aatggcacaa acaaatttag tcccacctgc ttctattgag ccatacattt
    16381 cttactccac cgtcctttta tctaggaatt cgttcttccc tgtctaatag tttttcgaaa
    16441 aactcatgtg gttccttttg gtaccaatag gcaacagaag aaatatcgtc ttgacgctca
    16501 taaagcttga tgtcatcatt tcctagggtt tggaaagtaa cacgtaaatt ttctttgaaa
    16561 ctaatggggt cgggtaaatg ccagcggtac aacccatgca tcggtaaagc atcatcgcca
    16621 aaaccatgaa ctggatttgg attaggtttt ccagcactaa aataatcccg ggtgtgatct
    16681 cttttagatt gaaaaggata gcccataaat agcgtattaa aattttgagc atatgccctt
    16741 tctccaaagt tctttttatg gaaagcccaa gcgccgccaa aataatcttc tgctcctgta
    16801 gatgttattg tagggaaatc ttcatctcca tctatataaa acttaaattc tccttccccc
    16861 caccagtatc tttccaatgc gcacaaagca aagtaggttc ctacataata cccagtgcct
    16921 ttgattccat caataagggt gtaatctttt ccttttgtcg tgatgggttc acggttccat
    16981 ttagcatgaa aatacatcgc atcatccggt acttcagtca atgcataatt aaatgtataa
    17041 aaaatgtgct taataaactc aggatgttca ttcgttaaag taatcttagc tttttttcga
    17101 aatggcatct caaaatagca attcatccct gatgttggat tcactacgat tggtaaggaa
    17161 ttcacttcgc atcttgtccc aaacccatta caaaaaaaat ctcctaatgg actttctaca
    17221 gaaggagtgg attcatcatc ccaataaata cgcaaaatta aatcacgtaa gacgaaactc
    17281 ccagcgattg ttttgtctgt cactgtcatc caaaaatgac gtaaaattcc agggccttcc
    17341 atttctgcta aagtaattgt ctctccagca tttatcggaa cagaaggtga cccttttctc
    17401 ccaattccta gatgactcgc tgtcattgcc gctttccctt tttcaccatt tttattttct
    17461 ggtgaaatag ttctactttc ctctcccttg tggagccata tattatttaa acaattcatt
    17521 ttattcgtcc tctatccttt cttcactttt ttgattattc atatagatga tttgttgttg
    17581 tgcctccgca ataattgctc caatatctaa attatcattt cctaactgca aagcgattga
    17641 cattgccaag ggaagattca ttcctgcaat tacgtgcact ctgtcagaaa tataggtaaa
    17701 aaacttttga tttacacttc ctccagccat gtcagttaaa acaattattt cgtctgtagt
    17761 atccactaac tctttaattt gttcatctat atcatcaatt tcttcgacat atgcacataa
    17821 gctactaatt tgattatgca tacctgttaa aaactctagt gtctctttaa aaccctcagc
    17881 tagtattgaa tggctagcaa tgatcaatct tctcataatt caaatccttt tcctttacgc
    17941 taatattcca aatgcagcca taaccatcgc aaaaacaata acgagtagaa tcaaaatggt
    18001 catactgatt tttttcttac tgagtagata atatgctgca gcagtaataa ttactggaat
    18061 taatgttggt aaaattttgt ccaacattgg ttggattttc atactaactt caccaaaagt
    18121 gaatttaagt ggcgttgaaa cactgataac tgaagcaatt aatgatccaa ctactgttag
    18181 ccccaatact gaagcagctt cagttaaata attaatcttt tcaccgtatt cagtaacaat
    18241 cttcattccc tgcttataac caaattcaaa gcctctcatt cggaaaaaga agattaccat
    18301 gattaaaatg aaccataaaa agattcctac aggattattt tctaaagcca tataagccgc
    18361 aattgatccc atgatggttg ggatcatcgt catcaaaaga gagtccccaa taccagcaat
    18421 tggacccatc gtaccaactt taaagtcatt aatcgcatct tttgcttcta atccttgctt
    18481 ttcctccatt gcaacacaag cacctaaaat aatggctgcc agccaaggct gtgtattgta
    18541 atactgaaag tgattattta aagcttcgat gtattcatca tcatctttgt ataattttct
    18601 taaaataggt gacaatgcgt aagtcacaga tgcaccttgc tgtgtttgat aattaaaggt
    18661 acaaactgac atcaaccaac gatagttcgc tttcctaaga tcctttttcg ttacttgata
    18721 tttttcgctc attttattca tcctccccaa taaacccaac atcagaagct cccgcagaag
    18781 cagtgatttg tgcatctttt tcagctctat gatacacatt aaatgctaaa gcgaatccta
    18841 tgatcgccgc tccaataata ggtaccttca aaaaagcggc taagaaaaat ccaacaagta
    18901 aataactaat gtatcgacct gtaggcataa agcgtaacaa catcgcaata ccaacagcag
    18961 gcaacatttt tccggctaat gttagcccat ccataaacca attaggtaaa acatccaaaa
    19021 cgtaattaac agcagactgt cctaaagtaa cggcaataaa tactggaatt gctgcagata
    19081 aaccaaagaa aattggcccc aaccacaaaa cactattcat tttcttaaat tctcgtttat
    19141 tcgcatattc ctgtgatttg cgaacaacaa aaccatttaa gattttgaca attacatcta
    19201 gttgcacccc taacatacct acaggtagtc ctattgctaa tccggcttct actccaagct
    19261 ggctaccaaa tgccgttgca ataattgcgc acgttccata gtccggtaca gatgagccac
    19321 caaaaccggc aactcctaat gacatcaatt gaactgtacc gccaactatc aaacctgttt
    19381 ttatatcacc catcaaaatt cctgtgaata atccccaaaa aactgcgtaa tttacaaaga
    19441 tcatcggaat cccataataa tcaacatgtt taataaatgc taatatagtc agcataataa
    19501 tttgaaaaat agtaaaatgc attatcctta ctcctttatc ctaattttga ttgaatttcc
    19561 tgtgccttat ccgccggaac aaattgtgta acaatttcta caccagaatc caacaatttt
    19621 ttatacgtgc tgatattttc atctgatgca taggctctag aagatagctg taatgcatta
    19681 tctttaacaa cagtaccgcc aacaattagt cggtcaattc taataccgtt atctactaaa
    19741 tgaaggattg tttccggctc tttcacgatg acaaatactt tttcctgatc gtatttcccc
    19801 tttttaaaat tcgcaatcgc tgtttcttca gtgataattg atacagccat tccagcaggt
    19861 ctagctaaac gcatacttga tttttggatg tcgttatttg cgacagaatc atcgatcacc
    19921 atcactctgt ttgctttaga cattggtgcc cattgcgtga caataatccc atgcaataaa
    19981 cggttatcaa ctctcgacat aacaatactc ataaatactt cctcctttat ctttcatttt
    20041 cttacaagca attaccatgc caaaacaaaa agaacttgat aaatcaagct ctttttacat
    20101 aatcataaac gtatttaacc tcatgaatcg ggatgtcgac actataataa tgttcaacga
    20161 cactgaaaga ttgcttaaaa tgtgtctgaa atttttgaaa ttcctcactc gtttcattga
    20221 aataaatttc atcttccaaa gtattttgtt ttactaatcg ttcaattaag caactaatgt
    20281 gaacatataa tccgaaacac gtattattat caaatgaaat tcctagatca ttttgtaaga
    20341 tgtatatagc atctgaaacc cgttctaata atttagtcgg atttaatatg gtcaattgct
    20401 ccattaaatt cgttagacta aaattcttca gaatattatt gttcaaaact ttcagttcat
    20461 tatcattaaa gtgtctattc aattgattgg agaatttact tatattcatt cctaaaatca
    20521 tatcttcaat tgaaaagaat tctacatctt ctagatgtgg atttagtgtt ccaacaataa
    20581 aaagaatatt gttctcttta cgaagctccg ataaatagtt ttcattcact aaagagtaat
    20641 agtcacacgt ttctatgaca agatcagtat cccttggaaa gctatcttca agtatctttt
    20701 ttaatttaaa cgaagttcct aatccgcttg cacaagaaca gacaatcata tccttcttaa
    20761 tatttgatga ttcataaata taatccacat tttgattttc ttttacttgt tcaagaatct
    20821 gtttaagcgg taaatctaat aatatctgat ttgcaacatc aatagccatt cttgttgaaa
    20881 cattgtttag tagtccgaat tttttttgag cgtctagttc caaatttaga taaattttct
    20941 ccagtgaacc catatcaaca agtaaaataa tttcttcata tccggtacac tcttgtaagt
    21001 atctattaat tttgtctgca atctcactac ttgaagtcat tagtggcata tcaatggcgt
    21061 cgtatatata tctctcaacg atcctattag cagtacttgc tatacaggtt gccgttagat
    21121 ctccatgagc caaaatcagt gctaaacgtt tttgctgaaa ttctttacta cacattaact
    21181 taaataaaat aaccaaaaca tttttttgaa tttttg
//
LOCUS       DAJSAW010000047        20488 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJSAW010000047 DAJSAW010000000
VERSION     DAJSAW010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.047"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="ONT64_001968"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="ONT64_001968"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HCR2303376.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="ONT64_001969"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="ONT64_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCR2303377.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="ONT64_001970"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="ONT64_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCR2303378.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="ONT64_001971"
     CDS             2857..3732
                     /locus_tag="ONT64_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HCR2303379.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ONT64_001972"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ONT64_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR2303380.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="ONT64_001973"
     CDS             4629..5063
                     /locus_tag="ONT64_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR2303381.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="ONT64_001974"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="ONT64_001974"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR2303382.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="ONT64_001975"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="ONT64_001975"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR2303383.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="ONT64_001976"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="ONT64_001976"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR2303384.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="ONT64_001977"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="ONT64_001977"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCR2303385.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="ONT64_001978"
     CDS             13334..14260
                     /locus_tag="ONT64_001978"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCR2303386.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="ONT64_001979"
     CDS             14442..15335
                     /locus_tag="ONT64_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HCR2303387.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="ONT64_001980"
     CDS             15469..15594
                     /locus_tag="ONT64_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2303388.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="ONT64_001981"
     CDS             complement(15728..16360)
                     /locus_tag="ONT64_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303389.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16508..17050)
                     /locus_tag="ONT64_001982"
     CDS             complement(16508..17050)
                     /locus_tag="ONT64_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCR2303390.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17185..17574
                     /locus_tag="ONT64_001983"
     CDS             17185..17574
                     /locus_tag="ONT64_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303391.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17578..18342
                     /locus_tag="ONT64_001984"
     CDS             17578..18342
                     /locus_tag="ONT64_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR2303392.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18471..19418
                     /locus_tag="ONT64_001985"
     CDS             18471..19418
                     /locus_tag="ONT64_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2303393.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19548..20288
                     /locus_tag="ONT64_001986"
     CDS             19548..20288
                     /locus_tag="ONT64_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303394.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7194 a         3481 c         4316 g         5497 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aattaaattg
    16381 ataagtatat aaagtataat aacaaattga tgagtatatg tcgatgtttt ttaatcaaaa
    16441 taataaataa aaactgtcta acatttaaaa tttaatttag atattagaca gtttttaagt
    16501 ttgtgagtta ttttccttgt cgttttctca cttttttagt ccagaaacca cctgaaatat
    16561 attttggctc ataagaaatg ataaaagcac gctcttcgat ttctttgatc aggcgataca
    16621 gttcacgttc gctttttcta ggagaaagta tttcgagaac tagccgctca ccttccagcc
    16681 ccattgcaga agtctgtgtc acaccataac cgtgttctct cagtataccc ggcaaacttt
    16741 tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat gatgtatcct aatgccaatt
    16801 tgtcttcaat caaaattccg acactgatac ctactgcgta acctaacgca tagacaaata
    16861 aattcagtgg attatctaaa cgatctaaaa caagggataa gccgacgata taaatcgtga
    16921 tctcgaccat actgacaagt ggagcaatca gtcgatagcc tttcattgtc aacatgaaac
    16981 gtaacgtgtt cagagtgata taagcaaaat taatgaggaa gatcatcccc aacatttttg
    17041 gatcaatcat ttgtaaaact cctttcattt atataacaac aatatcaaaa aaaatcgtaa
    17101 gtgaagaaaa attgaaactt tatacataca aaaaaatgat aaaatgatta taatagaatt
    17161 ataaagatag gttaggtgat taaaatgaaa acggtcaatc gtgtgttttg tgccagccac
    17221 gtgctaagtc tccttctcag tatatactta ggtgtctatt cgattcaaca gccaattgag
    17281 gtagagaaca ttgtttttca gatgtcgctg acagatggtt taattttaag cgtttttttc
    17341 tttttgatac tgtttattgg taatattttc ggtagcgttg ccagtttttt gaatttttct
    17401 atttatccac tcctttcgct agcattagga gtggtgggac tttgttctct gtgcttattt
    17461 cccgagccat tcagtcctgc atttatatta tttggtacac tgaacctgtt ccagacaact
    17521 gttggagcat ggttgctatg gcgatcagga aatttaatga aaataggtga gtaacaattg
    17581 aatccaatta tattgaaacg ttctgtccat catattccag tgttagaagt tgttctgaaa
    17641 gaattaagag aggaacgtat tcccgttgtc atctattatc acggatggca gacaagtaag
    17701 gagttagttt tgacgcaagg aagaaaaatt gcgcagcagg gcattcgtgt gatcctgcca
    17761 gatgcgatga atcatgggga gcgcaaacag ccggtttctt ctattccatc ctatacattt
    17821 tgggatagta tctacggaaa tctgtttgaa tttgacacac ttatcaagca tttagaaaaa
    17881 agaggtcttc tacatgatca gcttgcagtt ggcggggttt cgatgggggg aatgacaaca
    17941 tgcgccttgt tggctaaaca tcctcatata gtagcgggcg tgtgtttgat gggttcccct
    18001 gcacctttgg aatatggaaa agaaatcgta aggcgcgcat cggaatataa ttatgtcgta
    18061 ccaaaagact attttgattt gatttcatgg gttaagagct atgatttgtc attgcagccg
    18121 gaaaagttag ctggacgtcc tctgttcttt tggcatggac aagctgacga aaaaatccct
    18181 tatatacaaa cggaacgatt tgtagaaaaa aatcggcata agtcttacgg aaaagaaatt
    18241 gtttttcgtt cctcaaaaac acagcgacat atggtacaaa taccactaat ggaagaagct
    18301 tccacttttt tagcaaaaaa tttgataaaa aagtataaat aattgaaacg gaaaatcact
    18361 catgacgata gaggattttc cgttttttat tttgggctat aataaactct gttaggaaaa
    18421 tttggtataa ttgatagtaa gaatgacaac atgttagaaa ggaagagagc atgcccactt
    18481 tagcagatgt tgcaaaacgt gccaatgttt caaaaatgac ggtctctcga gtcatcaatc
    18541 atccggaaca ggtgactgac gaattgaaag agttagtttt ctcagcaatg gctgaactgg
    18601 attaccgtcc aaatattgct gcaaaagcac ttgtcacgaa tcggtcgcag atcatcaaat
    18661 tatttatact ggaagaaatc gacacaactg agccctatta catgaatttg ctgatgggaa
    18721 tcgctaaacg gatcggtaaa gcgcattatt ctctccaatt agttacaaat gatgccttcg
    18781 acgtcggttc ttgtgatgga tacatcatca caggtgtcag aaaaaaagac ttcgattgga
    18841 tcgaccgttt ggaaaaacca gttgtcttgt ttggggaaaa ttctgaagga tatgattatg
    18901 tagacagcga taatgaatat ggtacagcaa aagcaacgca atatgcctta gaaagagatt
    18961 atcaaacgat tatttatatc ggtatcgaag tggacgagcc ttttgaaatt tcaagggaaa
    19021 ctggttattt atctgtgatg aaggaagttg atcttcctag taaaatcttg cggtttccaa
    19081 atcattcgac agaagtagaa agatatatga cagaaaactg ggcagatttt acagataaaa
    19141 cttgttttgt atgtagttca gatcggctag cactaggagc agtaagaggg attataaatg
    19201 ctggaggaca cttgcccgaa gaatttggtg tgattggatt tgatggggta tttttagacc
    19261 aaatcagttc acctaaattg acaacgatca agcaagatat tatacgtatg ggtgaagtat
    19321 gtggagaaat gttgttaaaa aaaatagaag aaaaaggggc gagtcaaggt tatcgtcgtt
    19381 tttttcctga actagttatc agagagacaa cgaattaagt cttattacta tatatatatt
    19441 tatgaaattc atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat
    19501 ctgtgctatt attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca
    19561 gacgaaaaca tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg
    19621 acaagaagcg agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc
    19681 cgtttgaaca gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca
    19741 agcgtcgtgc aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata
    19801 aaggaaaatt accggattat accggccatt accaatcaga aatcacatcg acaggacgaa
    19861 aaaaactttt tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca
    19921 agcatactac acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc
    19981 ctaaatatgt acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc
    20041 atttaatcga atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga
    20101 cgccgtatca agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa
    20161 cgttagagga aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag
    20221 aacgctcatt acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt
    20281 ttaaataagt gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt
    20341 ttccttattt tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta
    20401 agcgaatttt ttctcccaat aagtaagaaa ataatggcaa aataaaaaaa caaactattt
    20461 tcgcagatcg tttgcaaaaa tagtttat
//
LOCUS       DAJSAW010000048        20414 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJSAW010000048 DAJSAW010000000
VERSION     DAJSAW010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.048"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            14..1093
                     /locus_tag="ONT64_001987"
     CDS             14..1093
                     /locus_tag="ONT64_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2303395.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
     gene            complement(1290..1991)
                     /locus_tag="ONT64_001988"
                     /pseudo
     CDS             complement(1290..1991)
                     /locus_tag="ONT64_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(2005..2929)
                     /locus_tag="ONT64_001989"
                     /pseudo
     CDS             complement(2005..2929)
                     /locus_tag="ONT64_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            3046..4125
                     /locus_tag="ONT64_001990"
     CDS             3046..4125
                     /locus_tag="ONT64_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR2303396.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            4157..4750
                     /locus_tag="ONT64_001991"
     CDS             4157..4750
                     /locus_tag="ONT64_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2303397.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(4860..5087)
                     /locus_tag="ONT64_001992"
     CDS             complement(4860..5087)
                     /locus_tag="ONT64_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2303398.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(5190..7778)
                     /locus_tag="ONT64_001993"
     CDS             complement(5190..7778)
                     /locus_tag="ONT64_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCR2303399.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(7798..8457)
                     /locus_tag="ONT64_001994"
     CDS             complement(7798..8457)
                     /locus_tag="ONT64_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR2303400.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(8506..9009)
                     /gene="trmL"
                     /locus_tag="ONT64_001995"
     CDS             complement(8506..9009)
                     /gene="trmL"
                     /locus_tag="ONT64_001995"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR2303401.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(9230..10096)
                     /locus_tag="ONT64_001996"
     CDS             complement(9230..10096)
                     /locus_tag="ONT64_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2303402.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            10205..10846
                     /locus_tag="ONT64_001997"
     CDS             10205..10846
                     /locus_tag="ONT64_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR2303403.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(10908..12269)
                     /gene="mgtE"
                     /locus_tag="ONT64_001998"
     CDS             complement(10908..12269)
                     /gene="mgtE"
                     /locus_tag="ONT64_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCR2303404.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(12287..13186)
                     /locus_tag="ONT64_001999"
     CDS             complement(12287..13186)
                     /locus_tag="ONT64_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR2303405.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(13188..13985)
                     /locus_tag="ONT64_002000"
     CDS             complement(13188..13985)
                     /locus_tag="ONT64_002000"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCR2303406.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(13963..14667)
                     /locus_tag="ONT64_002001"
     CDS             complement(13963..14667)
                     /locus_tag="ONT64_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCR2303407.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            14798..15373
                     /locus_tag="ONT64_002002"
     CDS             14798..15373
                     /locus_tag="ONT64_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCR2303408.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            15491..16168
                     /locus_tag="ONT64_002003"
     CDS             15491..16168
                     /locus_tag="ONT64_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCR2303409.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(16247..18055)
                     /gene="pepF"
                     /locus_tag="ONT64_002004"
     CDS             complement(16247..18055)
                     /gene="pepF"
                     /locus_tag="ONT64_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCR2303410.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(18231..19229)
                     /locus_tag="ONT64_002005"
     CDS             complement(18231..19229)
                     /locus_tag="ONT64_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HCR2303411.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(19455..20108)
                     /locus_tag="ONT64_002006"
     CDS             complement(19455..20108)
                     /locus_tag="ONT64_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCR2303412.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5908 a         4440 c         3639 g         6427 t
ORIGIN      
        1 aggatgaatt cacatgacgc aaacccatga taacacagtt tcacgcaagg gaaaacatct
       61 ttcctactca gaacgttgcc aaatcgctat cctgaaaaag gaaggctatt ccaaccgtca
      121 ggttgccaaa gtgctgggac gtgttccgca aaccataaac aatgagattg atcgcggaac
      181 catcacccag ttaaaacgcc aaacgcaaaa tggaaaagtg tatgacacct actattctgt
      241 ttatgacgct gatgcaggac aggccttcta tgaaaagcaa cgactgaact gtggcagacg
      301 ccccaaatgg gcgaacacaa atgccttcat tgattgggca gatgacaaaa tgttaaatga
      361 aaaatggtcg ccggacgtcg tggtcggatt tgccagaaaa cacgagctgt tcgattgtgc
      421 catcattcct ggcacaaccg ctctttatca atggattgac cgcggaatca tgcgaacaaa
      481 gaacatggac ttgcttgaaa aactgtcccg taaaatcaaa gcggtccgcc caggacaccg
      541 tcccaacaag cgtgttctcg ggacgtcaat tgaagaacgc cctaaagaag ttgaaacacg
      601 ggaaacgttc ggtcattggg aaatcgacac ggtagtggga aacaagacca agtcggatgc
      661 cgtgctgctg acgctggctg aacggcagac acgctttgaa gtcatcctaa agttgaacgg
      721 gaaggacacc gaatcggtgg accaagcggt tcaacagcta agagaacggg ctggcgctaa
      781 cttcgatcaa ttatttaaga ccgttacatc cgacaatggc tcggagtttt ctgggctaca
      841 cgatgctctg caagatgtag ctgacgtcta tttcagccac ccctatgctt cttgggagcg
      901 tgggacgagc gaaaaccagc acaaattaat tcgccgtttc gtccccaagg gaagcccgat
      961 tagtgaggtg agcgagaacc agattttgcg gatccagcgt tggatgaacg attacccgcg
     1021 gaaaattttg ggttatgcga caccacatga tacattcgtc caggcattca agcaagaacg
     1081 gttggtggct tgacccatta ttccttgata acgatcctca ctcgggacat ggcttcatgt
     1141 ctggaaagct ggcctggggc cagaatcgtc tgaagactat catacctcag ttcattatca
     1201 aggcctcact gatttagaat aacgaggcag ttacggtatc aaacaaatga gttacctgta
     1261 gtggctaact taaacttgaa atttacggac gtaaagcttg ctgccaaatt agcgattgaa
     1321 actaacgatt gctttctagg aatcaaggac aagcgaatac catttgacga ttgatttgct
     1381 tgctgatcct gtacattgcg gaccccccac atcgtcccgt agagaatcgc cattccaatc
     1441 agagtgaaga aataaaaact tttgaaactc atattacgac ctgcatgaac ttcttgaaaa
     1501 gatacttctt gttcaaaaac agtcatgatg ggcgcgagat caacgttctc cacttctgcc
     1561 actttactcg aaattatacc aatacgctgt aaaaattgat ccatgacgtt tttcatcaac
     1621 gtctgactca ttcctgcttg acccacatgg agcgaaacag atgaaccatc aatgagataa
     1681 aaaccagcca aatcatctgc atttagcttt tcattcgctt ctacttccga tagttcctgt
     1741 acttcaaata atggtttatc attgacttcg acttgttcta agactgtggt aaaattttcc
     1801 gaaagcgctg tttgatccgc tttgattaca attccagtat taatcgtttc gagattgcca
     1861 ccttgatcaa tctcgccaaa ggccgccata aacatcaatc ctaaaagaat tggaaaggcc
     1921 aatgtccaaa acaacatgga tttatttcgc agcagtattt ttgtgcgata tttataaaaa
     1981 tgccaaaaca ttatttcccc tcctctagtc tctcaaatcc ttgccagtaa tctctaaaaa
     2041 gacatcattc aaggacgctt ctttggttgc tatttttccg taactgatct tttcttcttg
     2101 taagattgta agaatcggca acaatactgt atttagttca gtagttgtta cttggagctg
     2161 acctttttta taagcgattc catcagaagc tattgttcgc tgacaacacg ttttctggga
     2221 gtaatgggat gtcgatcgtc aaacgatccg ccgtccgtac catatctttt agagattctt
     2281 tggttccttt agcaatcacc tgtccttgat ccatgatcac gatctggtcg caaagaattt
     2341 ctacttcttc catgtaatgt gttgtataga caatggttgc tccttgtcga ttcagctcct
     2401 tgatactttc taaaatttta ttccgacttt gggggtctac agctactgtc ggctcatcta
     2461 aaaaaattaa ccgcggcttg tgggcaatcc cacatgcgat gttcaaccga cgtttcaagc
     2521 caccactaag ttctcctgga cgaaacgaaa catatttttc taatcccact aattgaatca
     2581 cctcttcaat caaggctttc cttttctctt tttcggaaac atataaaccg caaaaataat
     2641 cgatattttc ttgcacactt agttcgtcaa agaccgcaac gtcttgagga acgaccccaa
     2701 tcattgcctt gacagcataa ttttcaggag tcattgttgt cccgaacaat ttaatggttc
     2761 ctttgtcata atttaacaaa gaaagcatac agttgatggc agtcgatttc ccactgccgt
     2821 ttggtcctaa cagacctaag acttctcctt cttgtacttg cagatcaaaa tgattcaatg
     2881 ctacaaaatc tccatagcgt ttgactaatt tatcaatttc gataatcatc atgaaaaact
     2941 ccttccaaat ttcttgttga tctgactata ataaagtgca agaaaagaaa ctagtgacga
     3001 ttggctaaac cacacatgac atttgtcaca atgaaggagt ctgctatgca taaatctcgg
     3061 ctactcaacg aaactagctt tctactggtt atctcattgc ttttgctggt aagtcaagga
     3121 ttcgttgcta tgcaagttat ctatttattg atcgccgttt tattcactgg tgtcagttat
     3181 ttactgccta caaagccaaa ttggattttc caagttattc tatttctttt atgtgccttt
     3241 ttacctgatc ttgtcctgtt tctgccagcg acgatgcgag tgatcgcccc tgaagaaagc
     3301 caccttgatt acttgttttt gttaggtttt ttgttgattt cgacggttgg taatcatccc
     3361 cccatcaagg tcgcctttgt cgtaattttg ctgttttttt ccttttactt acgaaaaaaa
     3421 gatcaacgaa cgaccgatct tgagaaaaag tacattgtct ccaaagatac tcattgggaa
     3481 aaacaacaag agttatcgga acaaaataaa gcattggccc tgtcacaaga aacacaatta
     3541 gccttgcaaa ttgtggaaga acggaaccga attgcccgag acatccatga caatgtcggt
     3601 catctgctgt caagtgcgat tttacaaatt ggtgcgcttg aaatgatcaa tcaagaacca
     3661 aaactaaaag aaccattaga aaatttaagt gccaccgttc acactgggat ggatcggatt
     3721 cgtcaaagtg tccatgacct ccatgagaca tcgatctctt ttcaacactc cttagagttt
     3781 ctgatcgctg attttccttc ttctgttacg attgaaggaa acttgtttga aggattgaat
     3841 gaaacgcagc aaaactgctt cttaatggtc atcaaagaag ccttaaccaa cagtatcaaa
     3901 catagtcatg ccaaaaccgt tgttttacat tttcgaacgt tgccggcctt ttatcgcttg
     3961 caaatcacga atgatggcac cagcccacaa aaaaattctg ctcccggtat tggtttgaaa
     4021 acgatgcgtc aacgtgttca agaacaaaag ggacaagtcc atattttcca agaaaaccag
     4081 caatttcagt taacgatcat tttaccaagg gagggacacc aatgataaca gtagtcatta
     4141 ttgagatgat ccatttgtga cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat
     4201 ccgtgtgtta ggcattggtc attgtgccaa ggatgcgcta gtcttatacg aaacccatcg
     4261 tcccgatgtt ttgctgacgg acatccgcat gcctaaacaa acgggcatcg atgctgccaa
     4321 agaaattttg gctaaatttc ccaaagccat ccttttgctt ttgacgacat tcaaagatga
     4381 agaatacatc cgcgaagcat tttccatcgg tgtcaaaggg tatttaatca aacagaactt
     4441 gcaagcgatt attccttctg tcaaatccgc ttataatgga caggccgtat ttggtaatga
     4501 aatcatcgag actttcactc aaatgatgaa gaataaaccg acgattgata gtcatgctta
     4561 tgcttctttt agtgaacgtg agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa
     4621 taaagaaatt gcagatgcct tgtacttgag tgacggcact gttcgcaatt acatcagcca
     4681 attactagaa aagctagatt tacgggatcg cacgcaattg gctatctatt tttatcagca
     4741 tttgcaataa gtcttatcta acacaaaaaa acctgaatga tgctccttcg catcattcag
     4801 gtttttttca catcttctat tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat
     4861 caaggattcg cagggctgcc aggattcgtt ggattgttcg gcgtgttagg cggaaaaata
     4921 ggcgccttgt ctaacgtttt ttctagtttt gattcttcac gatcgtctcg ttctacatca
     4981 ggactttcat tcaatttgaa ttctttttcc atctcatcta actttttaaa gtcattgcca
     5041 gattcttttg ctaatttcgc taatttatct tcggtttctt tagccataaa gtaacgcccc
     5101 ccttttgatt tcagcttagc atatcacact gaaaaaacaa aagaaaacgc taaccaacga
     5161 tcatttttag ctatttctca tctttaaaat caagcatttt ttgccggtat aaaatcgtag
     5221 ggagaactgt tttccatacc aatcatcgga tctactaact ggttatcgat cacttctgct
     5281 gtaagaacga aatctttaga aacagcaggt tctacctctt ctttcttttc ttctccaaca
     5341 aatgaagaaa acagatctgg ttccactgtt tccattactt ggtttttcgt ttcattcgtc
     5401 ccactgtcat cttctacagg tagcttttct tttacaaccc gtggctcttt tgtatagttc
     5461 ccatcttcaa atggatcatt tccgaccaaa gaatcttcat aagctgctag tttggtccgg
     5521 agagtgatat tcattttatc attatccatg atccttgatt tcaacgtagt catccggctt
     5581 gctgattcat tcgctacaca agtgacatag gcttgctctt cccctaacaa aatcaacagt
     5641 tccttactgc gagtcactgc cgtgtagagc aaattacgct gaagcatccg ctgatattga
     5701 tggaccatgg gcaaaataac catcttgaat tcactgcctt gtgatttatg gattgagcaa
     5761 caataagaaa gagtgatttt attccattcg tttcttttat aagtgacctc atttgcatca
     5821 aactggataa ctaattcatc cactttatct tcagaatctt ttgccaagat gatacccgtg
     5881 atttctccca tatctccatt gaatacattc aattcgggag tattgactaa ttggaggact
     5941 ttgtctccta tccgataaac ggtgtcgttc caatgtactt ccttttttcc aggtttccct
     6001 ggattgaata tctcctgcat cattttattt aacgcatcga tccctgcagg tccccgatac
     6061 attggtgcta atacttggat atcttgtgca cggaatccct tatttttcgc tttttccaca
     6121 attttagaga tcaagggttc aatttgataa gcatcacttc ggaaatagga acgatctttt
     6181 tgattctttg tgaaatcttt tggcagacgt ccttctttga tctcatgggc aagaggaata
     6241 atactagaac catcaccttg ccgataaatc tgattgagtt ccattttggg aatttgttcg
     6301 atttccaaaa gatcatggag tacttgacca ggaccgacag agggaagctg atctttgtct
     6361 cctactaaga taacttgcat attcgtcgga atcgctttga ataatgtgtt agccagccat
     6421 gtatcgacca tagacatctc atcaacaatc aaaagtccgc cttctaattc tttcgtactg
     6481 actgaaggtg ttttttcccg tccattcagc cccagaagac gatggatcgt actggcaggc
     6541 agacctgtcg tttcgttcat tcgtttagct gctcggccag ttggtgctgc aagtagaata
     6601 ggaaaaattt cttgggtata ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa
     6661 agattgacga ttccgttgat cacagtagtt ttccccgtac ctggtcctcc tgttaaaata
     6721 aatagagggg aacggattgc ttcagcgata gcttgttctt gcgaaggtcc atactgtatg
     6781 ccgaatattt tttcgatacg ccgaatattc ttctgcactt ctttttcagg ataatggatt
     6841 tcttttttac gactgagaag gcgctggatc gaattaccga tcccccattc agaaaagtac
     6901 aaactgtttt cgtaaagatt agtctcttct tgttgtatct ttccttcttc aacaagttgg
     6961 ataattacag tagcaacctg atcaggctgg atttcaattg ggcgacttgt ttctaaaata
     7021 cggatcgttt gctgaagcaa tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca
     7081 tgctgaaaaa tctgatgcaa gatagctgct cgaattcttc tcggtgaatc tgctgcaata
     7141 ccgatttgtt cggctagatt atctgcacgt ttgaacccga ttccttctat gtcttcgact
     7201 agctggtacg gattttcttc gatgatatcc aaagactcgt tcttataggc ctggtagata
     7261 ctgaaagcta actgactacc gaacccgtaa cgattcagtc ccatgatcac ttgatccatc
     7321 ccgtggttta gccgaatcgt ctcagcaagc atttctcttt ttggcttcgt caaaccggag
     7381 atttgtttta ataccgaagg atcagcaatg attttttcga tagcatcttc tcccagcaat
     7441 tctacaattt tttctgctgt tttcttcccg attcctggga atttttcgct ggaaagatac
     7501 gctacgatcc catttgcagt tgttggcttt tcttgctcat atctttcgag tttaagctgc
     7561 cttccgtatt taggatgatc cactaattct ccataaaaac gataagtctc attttcttga
     7621 atatcgccga aacttccggt gactacaatc tctttttcac ggtaatcagc tgtggtttca
     7681 ctgatgtgta ccaaaagcac tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca
     7741 actgtaccaa cgaaataagg catttcttct ggctgcatcg gaagccgcct ccttcttcta
     7801 tagtaaaggt tctggtgctg tttcagttcc taaaaaatct gtttggttcc atactttcag
     7861 ttcatatggc aataatctgc aaggctcttc agtctctaaa atcgtcaaac tactattgaa
     7921 caatccaccc atttcacgca actcacttag ctcttttcct gtcatccatt gaatggctgc
     7981 agttaaagaa gctccatgtc ctacgaataa aagtgggcca tctttatgtt gggcaaccgc
     8041 atctgctaca acagtttcga tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg
     8101 agaaggatca tacagatcta aatgattacg caaattcggc agattatccg gatatttctg
     8161 gtacatttca tcgatcgact gaccttctaa agtacccagc cctagttctc ttagcttatc
     8221 tgtataaaaa atctctaccg gacgagcaag ctctttgttg ataccttctg ctgtatcacg
     8281 tgctcgcttt gctgtactgg aataaattgc tttaaaagga acatatttta cctttttccc
     8341 aaatgctttg atttcttcgt aactttgtgg caaaagcgga gaatcgccat tgctcccttg
     8401 aaaacgtctt tcctgattcc attgtgtctt cccatgtcgg gtaaaatata gttccatacg
     8461 cagcaaactc ctgtctttct tttattttac tctataataa actgtctaat ccagcttgtc
     8521 attttcatag tgatgcgtca attctaaatt agggaatcct tgctggcgaa gtgcttcata
     8581 cactatcatt gcagctgtat tcgataaatt caatgagcgg acatgctcat cattcatagg
     8641 gatgcgtaaa cacttttctt cgttttcacg cataaatgtt tctggcaaac cggttgtttc
     8701 ttttccaaat aaaaagaagt gctcttctcc atcagcaaaa ttttcgtcac tatatacttt
     8761 atgggcaaat tttgagatca aatgtagttt acgattacct aaaaatgtta aaaaagcttc
     8821 taaattactg tggtaagtaa tatttacatc attccaataa tccaaccctg cccgttttag
     8881 atgtttgtca tcagttgaaa aacctagtgg ctcgattaaa tgaagcggag tattcgtggc
     8941 tgcacacgta cgggcaatat ttccagtgtt tgcagggatt aatggttcaa ataaaacgat
     9001 atgattcatc aggatcattc ctctcatatt tatgttcgtt tctattttct gacaatttga
     9061 tttttgcata aaaaaaaacg tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt
     9121 gaagctcttc tcactaactt ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca
     9181 acttgttgct aatataacac cctcaacttt tttttgcaac ctctactcat taaaaactgc
     9241 tagaaaaacg ttttctctta gttcctttta ataattctat gtcaattgta atcttcttca
     9301 aaggattctt atataagtct gctttgattc gctcttctac ctgccattct aaaggtgaca
     9361 tggccaaaag atctttttga ttttcctctg gtatctcaaa ttcataggtt acacgctgac
     9421 gttcagttaa tttcattgct tcagcaaact tggctactac acgttcattg gaataactct
     9481 gtttttcagg ttgattcgga taaaaagcct gacgtagttc ctttaaataa ttttcttgtg
     9541 gcaccacctt caacacttct ccatcttctg agagtacacg ttggaattct tggtaatgag
     9601 aaggcgaaaa gatatttaaa accgcgttga atgtttgatc agcaaacggc aaattggtca
     9661 aatcagctac acaccaaaat gcttcggcag attgttctgt agctgcgtag actccttctt
     9721 ttgaaatatc aaatccaaca gcattttgaa tctttttcaa ttttgaaagt tcattcaaga
     9781 agctaccttc tccgcagcca acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa
     9841 tagtctcaat aacgggctga tacatgccag attggatcat tctacgcctt ggaacgaaca
     9901 ttccttgatc gtattctgtt ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg
     9961 aaaggtcaaa acggtgatta tttttgcata tcaagccatt atcagacttt tttacttctt
    10021 cttggcaaac cggacagcgg aacagctgat gattttctgc caaaaactgg cgtcctcggt
    10081 caattttttt taacatagtt ccccctcatt tctgttttat cttaccgtta gatgctcact
    10141 ttggcaactg ctaaaaatat gatacaatgc ctagaagaaa gtgaaagata aaggagaaga
    10201 ttagatgatc aaagaatttt gcgctgaaaa ctacacatct attcctctag caattgctag
    10261 aggagccgat cggatcgaat tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac
    10321 tggtgtgatc gaagaaacgt taagttatgc tggggaaaaa gatatccccg taatgacgat
    10381 tattcgtcct agagggggcg acttcgtcta taacgatatc gaattgaaaa tcatgcatac
    10441 tgatttgatt gaagcaaaga aattagggac ggatggcgta gtatttggtt gtcttactcc
    10501 ttcaggctgg ctagacgaag aggccttaga actattgatc gaaacagcag aagggttgca
    10561 gatcaccttc catatggctt ttgacagtat tcctgaagat cggcaattcg aagcaatcga
    10621 ttggctggta gaacatggtg ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac
    10681 tattgtagaa aatttcccac ggcttaaaga attgattgcc tatacagatg gccgcttaat
    10741 cattttgcct ggtggtggtg tcactgcaga gaatgcagca catgtagcta aagaattgaa
    10801 cgtaagcgaa gtacacggaa ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa
    10861 aaacatttgc aacaggcata atgcttattg caaatgtttt tgttttttta cataaaatag
    10921 cccataaata aagaagcaat attgaaataa atcagtacac tcgtcaaatc gctcaaagtc
    10981 gtgataaagg gaccgcttgc tactgctgga tcaaatccta gacgatccat tagcatcggg
    11041 attaaactac ctgcaagatt cgccactgtg attgcaaata gcatagccat ccctatgaca
    11101 aaccccaaga taaaattatg tttccaaaaa cctacgatta gaaaaatcgt gatccccgta
    11161 acagctcctg tgacaacgcc ggtcaagact tcagccaaaa caagtcgaac aaatccatga
    11221 tctttctcat cagaaatcgc aagtcgacga acagccacag ctagcgactg ggttccagca
    11281 ttaccggcag ttcctgtaat cagcgaaatg aagactgcta aaatactcgc ttcacttacc
    11341 aagtcttcat agtgactgat taaagaagcg gtcgacatac ccaaaaacaa cagagtgatc
    11401 agccaaggca aacgtcgaga cgctgccttt actggatttt cattaatttc ttccacattg
    11461 accccggcaa gtcctgaata atcgctagca gcttcatcat caataacgtc gatgatatca
    11521 tcgactgtga cgattcctaa caaatgatca tcataatcgg taacaggaag agctaagaag
    11581 tcataatctc taaatgtttg tgccacatct tcttggtcgt cgcctacatg aactgaaagc
    11641 actcgttcgc tcatcagatc agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc
    11701 agagaaataa cccctactag ctgaccttct tgattaacga catagatgta atagattgtt
    11761 tcagctacat ctgcttcgtt cttcagcacg tacatcgcag agcgaacagt ttgattggct
    11821 acgatcgaaa caaattcagt tgtcatgatc gcgccagccg tttcatcctc gtaatggaga
    11881 agttccttga tttcactggc atcgtctgcc gacatcaaac ttaaatattt agcaatttgc
    11941 tttttatcca gcgtattaag caagtcaaca gcattatctg tatacatctc agaaagcata
    12001 tcagctgcat agctaggacg catttcatct aggtattctt tcatatgttc gttgtcttct
    12061 tcaatcacat cgaacatatc cgctaattct ttaggcgaca gataggaata gatcagctgc
    12121 ctgttctctt ctgtaagaga ttggtagaat tgcccctgct catagatatg catctctaaa
    12181 aaactgtttc tgaatgcctg aatattattt tcccttaaat cttgagtcaa ctgggcaaat
    12241 ctttcctcta attcttgatt ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc
    12301 gtctcacaac tgcctccatg tctgcggcta aaggctgctt gaactccagc aactcttctg
    12361 taaatggatg gtaaaaacgc agatcataac aatgaagggc ttgtcttttt attcccagat
    12421 ccattcgccc gccatacata tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa
    12481 ctcttatttg atgtgtcctt cctgtatgca gctggatatc aactaacgcg atttctgtta
    12541 gtcgcttctc tatccagtac tcagtctgtg ccgaacgtcc ttcatcagag accattcgct
    12601 tcaatagcga agaataatct cgggcgatcg gcaaatcgat atgtccatgt gccgataaat
    12661 ctgaaagcct tccactgact aaggcctgat attttttcac aaacttcttt tcacgaagtt
    12721 gcatatctaa ttttgcgtgc gcaaatccat gctttgcaaa aagcatcaaa ccagatgtat
    12781 cccgatctag tcgcgtcacc acatggatta cttggttttc gtaaccttgt tttttataat
    12841 aagcctttac acgattagcc attgtcccat ttggatggta ttgggcagga atagacgaaa
    12901 cgcctgctgg tttatttact accagaacgt gttcatcttc atacacgata tcgatcggtg
    12961 tttcatccaa aagtacggtt tcatgttctc cttcagcagg aatcacaatg gttaccttat
    13021 catccttagc tagagaaaac aagacatttt ctacttgatc attaactttg atttgaccgc
    13081 cttgaaattt tattttcgct aacagaccct ttgagatccc tttttctttt aaaaaatatt
    13141 ttacttgctg agaatgatct ttctcataat cccatgtaaa ctgcatatca atcctcacta
    13201 ataaaggcat ctttcacacg atgccaaaaa tgcatatgtc gataagaagc aaaatggatc
    13261 cgttcttctg ctattcgata ataaattgag cggatatttg cttttgcgac attcaattga
    13321 tcaaccgtta caagataatc ttctgtattt tccaatttta tttctagcca ttcatcatgg
    13381 gcaatcacga tcggagaacc caaagtacga aacactcgat tattcagtga agcaatctct
    13441 gccaactgaa aggcattgat actaggatgg atcacagcac cgccgacact tttattatat
    13501 gctgtcgaac cagtaggcgt agaaatcgtc aatccatctc ctctaaaact ttcaaacagc
    13561 tcgtttttga tgtatacatc ggctaccatc gtacgattgg cccgtttaat cgttgattca
    13621 ttcagtgcta aaaaatgctg gtccggtgtt tcatccaaat aactgatccg aacatccagc
    13681 aagggataac taacactttg ttcccggttc gttcgtaagc tgtctactag ttcttccagc
    13741 tcgtaatccc gccaatccgt gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc
    13801 acatcattca gacaatgact gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg
    13861 actgagatga ccaattctgg ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt
    13921 gcgttcaaac gttctgtcac ttctatagtc tttgcttctt tattatgaac gatcgctact
    13981 ttcatcccct gcctcctctc tttcgctttc tttcttcaaa agcgttcgat agtagacatc
    14041 atctgatttt cctcttccat gggagaataa ccgctgtgct tcttggattt cttcacggat
    14101 actggacatc tcttcatcca actgataggc agcttctgcc gcacgctgaa gtcgttcact
    14161 catttcttct ggaaaaacgc cttgatattt ataattcagc gaatgctcaa tcgttgccca
    14221 gaaattcatc gcaagagtcc gaatctggat ttccgccaaa attttcttct caccgctgat
    14281 cagctgtaca ggatactcga tcaccagatg ataagaacgg tagccacttg cttttttatt
    14341 tgtgatgtaa tctcgttctt ggagaacttt catgtctttt cttttacgga tgatctcaac
    14401 aacttgatgg atatcttcta caaactgaca catgatcctt aacccggcaa tatcagacat
    14461 ttcttcttct aaacgtccca ttggaatatg ccgtaattgt gattttgtta caatactatc
    14521 gactggcttg actctcccag tgacaaattc aatcgggaca tgtcgatttt gttcacggaa
    14581 ctgtttgcgg atcccccgca gtttcacttt caattctgat acagtttgtt catatggtgc
    14641 taaaaatgct tcccaatctc gttccatagc tgatacctct acttcattaa cattcattgt
    14701 ttattctacc atatatgcaa agttttttcg tactcttgat ttatgcattg accaaaaaaa
    14761 gaaaattagg caaaatagac aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga
    14821 atataaatct atgctgacaa agcaagagta cgagatattc atcgctcact atcaattaac
    14881 agataaagat ttccgtgtac agactaatat ttatttcgat accgttgatg aacagctgaa
    14941 gaaacaaaat tgtggcctac ggatccgttt cgttggtcat caagctgaat atacattaaa
    15001 aacacctgct gaaaaagggc gtctcgaaac aacggatacc tttataacag aagaagcaga
    15061 agcatttatc ttcgaaaaac gtctgcctcg ttcaggcgca gtttttcaaa aactttctga
    15121 tttgaatatc gatcctgctt cattaatcca aaccggaaag cttacaacaa aacgggctga
    15181 attccctatt gaagaaggac ttttagctat tgatgaaagc tggggagaca atctacacga
    15241 ttttgaactg gaactagagg taggggatgc atctgtggga aaaattgctt ttataaattt
    15301 tttaaaacgt ttttctttac cttatcgtcc tgctaagaac aagatccaac gtatgcttga
    15361 agctaaaaat tagaaacctt tcattttcac aaggcacttg agttcatttt tttacttttt
    15421 tctgttaagt tataggatgt gaaagaatct agtcactagt tgtatagaat ctattttggg
    15481 ggagattcat atgatcgaaa tctatttatt tgttaatcct ttaggggaat actgtttcga
    15541 aatggaacaa cagttattac aatttatcga agaagagtac gggacaccat caaaagaaaa
    15601 gatgcaattt cgctttcttc ctttggtcaa tttgcagaca attggggatg tcatgcaaca
    15661 aaaaggaatt tctcagaaca atcttgagga acgtaatcac ttattctcaa tgacttattc
    15721 tgcagcattg gactgcaaag cggcgcagtt tcaaggaaag aaaaaaggcc gccgtttttt
    15781 gatcaaattg caagaagcag tcggctgtaa tgacatacca tattctcaag aacttgctga
    15841 atccatattt gaagaaatcg gcggcgattt gaatatgttt aaagaagatc gtcagtctga
    15901 cttcgttaaa gaagtattct tttctgatca aaaaattgct cgggaaatgg gcatcacaaa
    15961 acacccttct gcagtcgttt acaattatgc ctgcgaccgt gattttggtg ttttagtaga
    16021 agtaaaaggt acaagtagcc tttggagaat cacagagctg tgtaaaacaa cagataactc
    16081 atatcaagtt tttaatggtg aacatttaca tacaaatgat tctcagttcc attcaagaac
    16141 tagccattta cacttgcttt ctaattaaaa aaatacgtcg actcccttac aagagagccg
    16201 acgtattttt ttcgtttcta ttcgagaaaa tcaaaaatag aaagccttat aaacgatcga
    16261 tcaattcttc taattcattt agacgttgtt caaacatcga cattgcatct tcaatatatg
    16321 cagcctgtgt catatcaact cctgcttttt tcattacttc cacaggataa tcgctgttac
    16381 ctgcttttag gtaagccaag tagttctcta gtgcctctgg ttcctggttt agtattttct
    16441 tcgccagtgc tgaagcagca gaaaagccag tcgagtattg gaaaacatag taattataat
    16501 aaaaatgcgg tatccgtgac cattcgaatt taatttctgg gtcttcttcg actgctggac
    16561 cataatattt tgcattcaat ttaccataac tatcacttag gtattcacta gtcaatggca
    16621 cacctttttc atcttccgta tgcatgaaat gctcgaattc agcgaattgt gtctggcgga
    16681 aaactgttcc tttaaaccca tccaagtaat ggttgagtac ataagcccgt acacgagggt
    16741 ctttttctgt ttccaataaa tactccgtta ggatattttc attcgttgtc gaagcaattt
    16801 ctgctaaaaa gatggagtag tcgccgtaca cataaggctg gttcgaacga gtgaaataac
    16861 tatgaacact atgtcccatt tcgtggacaa gcgtaaatag ctgatccagc gtatcatgcc
    16921 aattcaataa aatatatgga tttgtgtcat agcttcccga agaataagca ccgcttcgtt
    16981 tccctttatt ttcgacaaca tcgatccaac gttcagagaa tgctttttct acgatggcca
    17041 tgtattcttc acccattggt ttcagtgctt ctaaagcttt ttcttttgct tcttcgtacg
    17101 taaaggtaat tggagcttcc cctaagaccg gtgtataaag atcatacatg tgcagttttt
    17161 ccacttctaa taaacgtttc cgtaattcca tgtatcgatg taacaaaggc aaatgcttgt
    17221 ttaccacgtc taccaaagta tcgtatacac tttcaggaat atgattattg ctcaaagacg
    17281 cttctctggc agagctgtaa ttacggactt tcgctttaaa attatgtcct tttatatgtg
    17341 tgcctaaagt agaagcaaat gtatttctaa attgttcgta aacactgtac aatcccttaa
    17401 atgctgcttc gcgcaccctt cgatccgtgc tttctagcaa ctgaccatac acgccatgag
    17461 ataattggac tatttcacca ttttctcctt caatcgttgg aaaaactaga tctgcattat
    17521 tcaaaacagc aaatgtatca cttgaagcat caaagatttc acctgctcca gcaagaagag
    17581 attcctgctc agcagataag acatgggctc gattatctac tatttgctgg atataatggc
    17641 gatagacttc caattttggt tcttctttga aatattgcca aatctggtca tctgacaatt
    17701 gcaatatttc tggttcaaac caggaaacgg cttcactaac tttcgaaaac agactgcttg
    17761 ctcttgcata aagcgcttgg taatctgtat ttccagtgtc ttgatcgttt ttaaggtgcg
    17821 catatacata aatgacttca gtttggcgat aaaccctcaa tacgaattca atcgcattta
    17881 aaaattgaga agcgccttga tcaagtgttc ctttgtgttt ttcagattgt tttaactctt
    17941 ctgataattc caaatatttc tcatcaaact cttggtcgct tgaaaagatc ttggtcaagt
    18001 cccaagttaa attttcaggc aattcttctc gttttggcaa ctgctttact tccatgaaat
    18061 ccactccttt tcttccctta gctctatcta tcaaaataga tcatagtcta acatccacat
    18121 gtcacagcat ctaacttttc tactcattca tgtgtctgct tccatcaatt ttagcataac
    18181 taaatcttgt tttacataga aaaccgattc ctagcctatt tttatactta ctattttttc
    18241 aaagaagaca agtgtatgca ttcgttaaaa aactgctgat agaccatctc ttcatcaatc
    18301 ataggaaaaa gccattccct tttatgttct tttatttggc aaagccatga gttgaaatca
    18361 ggaactttct ggttttccaa ggcttcttcg tacaacattc gataaagaaa aacctgttct
    18421 tgtaaataaa agaaaaaccg acttttttga tagatccacg atgatagatg caaaagatgt
    18481 ttatgacgca agtagcattg ttcttgtatg gatactgtat tcttttgctt gtgaataagt
    18541 tggcgtgtca gccatttctt cccatcttca ctcacttttt ctattttttt catttggttc
    18601 attttaattt cgttactagt gaatatttct ttaaggtccc tactagaaaa agtccagcag
    18661 ctactgcaaa aggaaatatg ccctgataca gtctctgtaa tatgatgata caaataaagc
    18721 ttttgtttct tgagatcagc tttccagcaa tgcactcctc tttttctatt atagtagcag
    18781 caactttttt ctatcaatga gaattgcgat tgtccaagaa aagaattacc aagtatccac
    18841 catacttggt aactgttatt caaataattt tgggttcttt caataaatct ctggtgagac
    18901 agtctagaac attggatttc tattgctaga ttcgccaaca aaatatccgg cctttgccgc
    18961 aacctcggca aataggcttc caaaaatact ggttctactg actgtcccag ccaatccatg
    19021 aagcattcct ttaactgtaa gtgctctttt gattctcttt cactaaagac cgaacagtta
    19081 tctgattttt gatgggcaaa atgagcacat tttttctttc cctttttcaa aatcactctt
    19141 tttcgacagc cacgacaata atatttcttt cctttttctg catgtttagc aaaacagcac
    19201 ttattatatt cattcattgc aagcagcatt tttctccctc tgaacttatt tacgtctaag
    19261 aatttattta cacgaaaaaa gagggcgtga aaaagctatt ctgcatcaag taataagaac
    19321 agctaaacaa aaatagttcc caatattttt cgtttggttg ggcttgttac cgcagatgca
    19381 agcttttgaa cacatttata cgaaaaaaag actgcgacac aatttttgtc acagtctctt
    19441 actataatgc tgatttatac aaaataaaat ttcgttcgct cgatagcatt acgcgccatc
    19501 agacattgtc catattcatt caatgtttct ggtgtacgat cgctcttttc agcaaactcc
    19561 aaaattcgag caatctgatt ctctacatcc gcttggttga gattttccat ccagaagtga
    19621 acgactaggt aatagcgctc ttcgaataaa tacaaatcag tccaagcaga ctctagttcg
    19681 acatctgttg caagttggat cacttgttca aaatcttcaa aagcaaaagt tgcttgtctt
    19741 tcctgttctt ctagggcgct tccagataat tcttcctccg gatcgctgcc aataatctgc
    19801 tgacgaagaa agtcactcac atcttctgaa tcaacagtag aatcttctga ttgagaaatt
    19861 tcttcatccg gaagattttt actgataaac agttctaatc cgtctccttt tggcaaaact
    19921 tggaaagtga cagcttcgct tcctcggaac tcctcttcga tatcaacttc ttccaaaata
    19981 ctataaaaga aattttcgat ttctttgtga ttgccaagta aatccaaaaa tgaaacgccc
    20041 cgttcctcta aatcttcatg accaattagt acacggatcg tattttcatt aatatgttcc
    20101 atttccattt tgctacacct cactttgcca caatgtttaa tatagattca ttgtagcgaa
    20161 atcagcttaa atgtaaagaa aaaaaatctc gctgttaagc gaatttaata aaaccagcgg
    20221 atttttcaac ctttcaagct atcttattta actataacat ttttggcaga cgaatactat
    20281 atagccgact tttcagctaa ttttccctct ctatttttcg tgcaatcatc atcttctact
    20341 acctttttat acatctaatc aagaatactt tttgtaaaaa caaaaaaact tggaaaaact
    20401 atctttgtaa tagt
//
LOCUS       DAJSAW010000049        20177 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJSAW010000049 DAJSAW010000000
VERSION     DAJSAW010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.049"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..152
                     /locus_tag="ONT64_002007"
     CDS             <1..152
                     /locus_tag="ONT64_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2303413.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="ONT64_002008"
     CDS             complement(230..763)
                     /locus_tag="ONT64_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCR2303414.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ONT64_002009"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ONT64_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR2303415.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ONT64_002010"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ONT64_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR2303416.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="ONT64_002011"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="ONT64_002011"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR2303417.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="ONT64_002012"
     CDS             complement(4488..5066)
                     /locus_tag="ONT64_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HCR2303418.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="ONT64_002013"
     CDS             5388..5858
                     /locus_tag="ONT64_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HCR2303419.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="ONT64_002014"
     CDS             complement(5966..7192)
                     /locus_tag="ONT64_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR2303420.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="ONT64_002015"
     CDS             complement(7946..8146)
                     /locus_tag="ONT64_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2303421.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="ONT64_002016"
     CDS             complement(8280..8888)
                     /locus_tag="ONT64_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCR2303422.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="ONT64_002017"
     CDS             complement(8973..9443)
                     /locus_tag="ONT64_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR2303423.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="ONT64_002018"
     CDS             complement(9491..10405)
                     /locus_tag="ONT64_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR2303424.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="ONT64_002019"
     CDS             10551..11354
                     /locus_tag="ONT64_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HCR2303425.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="ONT64_002020"
     CDS             11532..12479
                     /locus_tag="ONT64_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR2303426.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="ONT64_002021"
     CDS             complement(12547..12840)
                     /locus_tag="ONT64_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HCR2303427.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="ONT64_002022"
     CDS             complement(12868..13533)
                     /locus_tag="ONT64_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCR2303428.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="ONT64_002023"
     CDS             complement(13673..14305)
                     /locus_tag="ONT64_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2303429.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="ONT64_002024"
     CDS             complement(14312..15379)
                     /locus_tag="ONT64_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2303430.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="ONT64_002025"
     CDS             complement(15376..16107)
                     /locus_tag="ONT64_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCR2303431.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="ONT64_002026"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="ONT64_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR2303432.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="ONT64_002027"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="ONT64_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HCR2303433.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18270..19439
                     /locus_tag="ONT64_002028"
     CDS             18270..19439
                     /locus_tag="ONT64_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCR2303434.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(19706..>20177)
                     /locus_tag="ONT64_002029"
     CDS             complement(19706..>20177)
                     /locus_tag="ONT64_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303435.1"
                     /translation="VVNMVKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLK
                     NHQVTEDGQVIYSSFSPNKDEIIFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQ
                     GGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         6105 a         4025 c         3208 g         6839 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    18241 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    18301 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    18361 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    18421 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    18481 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    18541 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    18601 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    18661 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    18721 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    18781 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    18841 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    18901 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    18961 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    19021 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    19081 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    19141 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    19201 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    19261 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    19321 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    19381 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    19441 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    19501 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    19561 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa
    19621 gatttctctt taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta
    19681 gattgctaaa atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc
    19741 aataaaaaag aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa
    19801 atgtgtatga atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa
    19861 aaggacctcc ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt
    19921 tataccggaa tacaggtaaa actattgttt tgccttttga agatttaaac gtaaatataa
    19981 tttcatcttt atttggagaa aagctactat aaataacttg accatcttct gtaacttgat
    20041 ggtttttcaa aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa
    20101 attcttcaag tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt
    20161 tcaccatgtt aactact
//
LOCUS       DAJSAW010000050        19821 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJSAW010000050 DAJSAW010000000
VERSION     DAJSAW010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.050"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            6..305
                     /locus_tag="ONT64_002030"
     CDS             6..305
                     /locus_tag="ONT64_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2303436.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ONT64_002031"
     CDS             378..1739
                     /locus_tag="ONT64_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR2303437.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ONT64_002032"
     CDS             1761..2021
                     /locus_tag="ONT64_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303438.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ONT64_002033"
     CDS             2035..2787
                     /locus_tag="ONT64_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HCR2303439.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ONT64_002034"
     CDS             complement(2881..3486)
                     /locus_tag="ONT64_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2303440.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ONT64_002035"
     CDS             complement(3539..4513)
                     /locus_tag="ONT64_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCR2303441.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ONT64_002036"
     CDS             complement(4891..5610)
                     /locus_tag="ONT64_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2303442.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ONT64_002037"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ONT64_002037"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCR2303443.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ONT64_002038"
     CDS             7161..8558
                     /locus_tag="ONT64_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2303444.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ONT64_002039"
     CDS             8639..9079
                     /locus_tag="ONT64_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR2303445.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ONT64_002040"
     CDS             9227..9553
                     /locus_tag="ONT64_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR2303446.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ONT64_002041"
     CDS             9554..10303
                     /locus_tag="ONT64_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303447.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ONT64_002042"
     CDS             complement(10383..10838)
                     /locus_tag="ONT64_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCR2303448.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ONT64_002043"
     CDS             complement(11265..11540)
                     /locus_tag="ONT64_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303449.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ONT64_002044"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ONT64_002044"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR2303450.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ONT64_002045"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ONT64_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR2303451.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="ONT64_002046"
     CDS             14770..17436
                     /locus_tag="ONT64_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR2303452.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="ONT64_002047"
     CDS             17800..18552
                     /locus_tag="ONT64_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR2303453.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="ONT64_002048"
     CDS             complement(18574..19728)
                     /locus_tag="ONT64_002048"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCR2303454.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT         6330 a         3473 c         4060 g         5958 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat t
//
LOCUS       DAJSAW010000051        19476 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJSAW010000051 DAJSAW010000000
VERSION     DAJSAW010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.051"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(22..996)
                     /locus_tag="ONT64_002049"
     CDS             complement(22..996)
                     /locus_tag="ONT64_002049"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR2303455.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1076..1951)
                     /locus_tag="ONT64_002050"
     CDS             complement(1076..1951)
                     /locus_tag="ONT64_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HCR2303456.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2081..3988
                     /locus_tag="ONT64_002051"
     CDS             2081..3988
                     /locus_tag="ONT64_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HCR2303457.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4529..7807)
                     /locus_tag="ONT64_002052"
     CDS             complement(4529..7807)
                     /locus_tag="ONT64_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HCR2303458.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8078..8623)
                     /locus_tag="ONT64_002053"
     CDS             complement(8078..8623)
                     /locus_tag="ONT64_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR2303459.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8635..9327)
                     /locus_tag="ONT64_002054"
     CDS             complement(8635..9327)
                     /locus_tag="ONT64_002054"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR2303460.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9346..9630)
                     /locus_tag="ONT64_002055"
     CDS             complement(9346..9630)
                     /locus_tag="ONT64_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303461.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9643..10206)
                     /locus_tag="ONT64_002056"
     CDS             complement(9643..10206)
                     /locus_tag="ONT64_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR2303462.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10434..11096
                     /locus_tag="ONT64_002057"
     CDS             10434..11096
                     /locus_tag="ONT64_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCR2303463.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11220..12416
                     /locus_tag="ONT64_002058"
     CDS             11220..12416
                     /locus_tag="ONT64_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCR2303464.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12517..13893)
                     /locus_tag="ONT64_002059"
     CDS             complement(12517..13893)
                     /locus_tag="ONT64_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2303465.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13930..14796)
                     /locus_tag="ONT64_002060"
     CDS             complement(13930..14796)
                     /locus_tag="ONT64_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HCR2303466.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(14885..15910)
                     /locus_tag="ONT64_002061"
     CDS             complement(14885..15910)
                     /locus_tag="ONT64_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2303467.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15928..16602)
                     /locus_tag="ONT64_002062"
     CDS             complement(15928..16602)
                     /locus_tag="ONT64_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2303468.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16786..17679)
                     /locus_tag="ONT64_002063"
     CDS             complement(16786..17679)
                     /locus_tag="ONT64_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR2303469.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17753..19450)
                     /locus_tag="ONT64_002064"
     CDS             complement(17753..19450)
                     /locus_tag="ONT64_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR2303470.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         5684 a         4189 c         3445 g         6158 t
ORIGIN      
        1 ttctttccct cttttttttg tttattcgaa ttctttttct tccagacgga ataacggact
       61 catcggtgta ttttcatgga tgcgtttgac cgcttcaccc attaattctg aacatgtaac
      121 gattgttaag ttttctggat gacgattttc ttctgtgaaa acagaatcag tgatacaaat
      181 atctttgatt ggtgcattgt ctaaaatact tttcgcatga tcggacaata atccatgtga
      241 cgcacaggca tatacttctt ttgcgccgtt ttcttttaat acatttgctg ctcgtgcaag
      301 cgttgctcct gtgttcaaga tatcatctac catgatacat gtctttcctt gaacatcacc
      361 gatcacataa ccaccctcag ttgaagcatc atctgcatga tctacgatgg ctaatgtgct
      421 gttcaaatat tctgaaagac tacgtgcacg ctgaacaccg ctgttttttg gcgaaacaac
      481 tacgatgtca tctcccgtca gacctaattg acggtaataa tgagcaaaca acggcatcgt
      541 aaataaatta tctacaggaa tatcaaagaa tccttgtact tgaaccgtgt gcagatctaa
      601 agtcagtact cgagttgccc ctgcttcttc gatcaagtta gcgatcaatt tcgcagtgat
      661 cggttcatga ggtttcgccg tgcggtcttg cctagcatac ccatagtaag gcaatacgac
      721 attgatcgtt ttcgcacttg ctcgcttcat tgcatcgatc atgatcagta gttccatata
      781 gtagtcattg acaggtgcat tcgttgattg aacgatatac acatgatccc ctcgaatact
      841 ttcttcgata ttgatggaaa tctcgccatc actaaattgc ttcacgacgc ttttacctaa
      901 ttctgttccg aacactttcg cgattttttc agctaatgga cggttgccat tcaaactaaa
      961 aatcttcaac gtgtcatcct gatacttatt actcatgtag tcctccccaa aacttctcta
     1021 ttcttcgata gtttaacata tttttccaag attttctgtt aatttcagca aattcttaag
     1081 gaagtgaaac tgttccgtta ctccaactgg caggaatgac tttatgataa tcgccccagt
     1141 tgtcgtgtac tttgtaataa cgttctcctg tttcagtttc aaaataagcg tacgctgtca
     1201 cccaatgatt gccatatgta ctgccaagta ctttcaaaat accaatcatc acaggtttgc
     1261 cttcacgtat acgttttgta gctcgctgcc atgaacctag aagagttgct cgtgcacgct
     1321 catgattgcc attcttcttt aaaaaactag aaatccctgt actaacctga aaaggaatcg
     1381 taggaagtcc tagtggttgg atctggctac gcaatgcctt cgtcaaaacc aacgaatcag
     1441 ccgtattttt cttgcgaatc tcatttggaa gcatccaccc cttacgatag tcttgataat
     1501 aggcaaggag aactgctgcc gcgtaagtac cacaaagata gcctgagggg gtagcccatt
     1561 tcttatacct caaaaaacgt tcttctggca atcccaccca gcaatttgcg agttcctttc
     1621 ctgacctttt ttcttcgatc aaaacgggaa tatcttcttt tctgttgatc catctagata
     1681 actcgattgg ctcttcattg atcagcttcc attttttaca agaaagaccc tcttgataag
     1741 tatattttgc tggagaatat tctaccggat gtcctgattg atcgataatc acataacttt
     1801 gatcttcaaa aaccagagct aaaaaaagca atttactttt tcttgactgc agctgctgag
     1861 tgctggctaa ttttttaatt tttttatcta ctttgaaata taaaggttta tccactactt
     1921 gattcagttg ggatcgcctc ctttttttca ttataagtga aggattgaaa aaaacaaatc
     1981 agtttgattg ttcgaatttt caatttttta tgttctattt gttaattcct tcattttgta
     2041 agataatggg cacacgaaac aaaaaaggag cgatcgatga atgaacaaag aacgattgaa
     2101 acaagatttc tatgaagcag tcaatgaaga atggcaaaaa acagcaaaaa tccctgcaga
     2161 taaaccagct acaggcggtt tccaagattt agttgacggg atcgaccaac ttttgatggg
     2221 agaaacagac gatctgcttg cccatccaga aaaagtgtct tcagaaaaaa tgaaacattt
     2281 ccttgcatat tatcaactgg caaacgatta tgaccgaaga aatgagttgc atgctcaacc
     2341 gcttcaacct ataatcgaaa aagttgaatc attgcagtct ttcgacgaat tgaataagca
     2401 atttccagaa tggctgttgg atacattacc attgccattt aatctggatg tagatgcaga
     2461 tatgaagaat gcgcaaacga acgccttctt tgcttatccg ccttctttat tccttccaga
     2521 taagacgtat tatgcatcag accatcccaa cggtgcacaa ctaataggcg ttttctttga
     2581 tatggctgtt cagttattag agcttgccgg caaagaaaaa agtcaggcag aagcaatcac
     2641 ggaacaagca gtgcaatttg ataaattgat tgctcctcat gtaaaaagtg cagaagaaaa
     2701 cgcagattac agcaaaatgt ataatccgca atcttttgat acatttatta aatacacaga
     2761 taaactggac ttgaaaaaac ttgtcacggg tttgattggg acaactccag ataaagtgat
     2821 cgtaactgat ccagtgtact tcgatcattt gaatgacatc ctgacagaag ctcattttcc
     2881 gttattcaaa agctggatga tcattaaaac agtacgttct cttagtggtt atctatcaga
     2941 agaattccgc caagtcagcg gaatctattc tcgcgtattg tctggaacag acgaagcaat
     3001 gcctcagaaa aaagcagcgt attaccttgc ctctggacaa ttcgatcaag tagttggcga
     3061 ttactacgga aaaaaatatt ttggcgaagc tgctaaaaaa gacgtagaac aaatggtgaa
     3121 aaaaatgatc gccgtttata aaaaacgtct agaagaaaat gactggcttg gggatgcaac
     3181 aaaagaaaaa gccgtcataa aattaaataa cttaggtatc caagtaggct accccgataa
     3241 gatcccagca ttattcgacc agttcaaaac agtacctgca gatcagggtg gtactttgct
     3301 ttcaaatact cttgttttca gcaaactcac attaaaagat cgtttcagca aatggaacaa
     3361 accagtggat cgttcagaat gggaaatgag tgctgacacg gtcaatgctt attatcatcc
     3421 atttcggaat gtgatcgtct ttccggcagc gatccttcaa gcaccatttt acagtctaga
     3481 acaatcaagc tctgctaatt atggtggaat cggagctgtt atcgctcatg agatttccca
     3541 tgcatttgat aataacggcg ctttatttga cgaatatggc aacttgaaca actggtggac
     3601 agaagaagat cttacacaat ttcaaaacaa agcacaagca atgatcgaag aattcgatgg
     3661 tctgcctttt gctgacggaa aagtaaacgg aaaattaacc gtttcggaaa atattgctga
     3721 tgcaggcggt ctaagctgtg cattggaagc agcaaaaacg gaatcagatc tttctatcga
     3781 ggaattcttt gtcaattggg caacgatttg gcgtacaaaa gcaaaaaaag aataccaaca
     3841 acttttactg caaatcgatg tccatgcccc tgctaaactc cgagcaaacg tgcaaccaaa
     3901 aaaccttgac gaattctatg aaacatttgg tatcacggaa caagacgcta tgtatctagc
     3961 gccagaaaaa agagtgcgaa tctggtaaga ggttgtgcca gaagtggtca gcttcaagaa
     4021 ataagaagga atttgggaaa atcgcttctc aaatttttgt gacatttcgg cttatttttc
     4081 gaagaagtta cttctggaat accatgtatt agaggttgtg aaaaagctgc ttagtttcaa
     4141 gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt tgacttacta
     4201 ccgagaaact ggcttttgaa caccgtttat cgagaggttg tgaaatcagc gtcctgacct
     4261 gagtattaga gaaaaaaacg gaaaaatagt tcctcatatt ttgccgtttg ttgagctaat
     4321 accgagggtc agctgattga acaccgttta gtggagagat tgtgaaaaag ctgtcctgca
     4381 tcaagtaata agaacagcca atcaaaagca gcaaggagaa cctcatctat aaaaagatag
     4441 aaggaagtga gacatctatt ggtctcattt ccttctattt ttactttcct cagtcaattg
     4501 ttttacatca taaaaggaga aattccactt atttttttcg ctgtttcatg acataaacaa
     4561 ctacaccgat taacaaaatt cctccagcta atgctagcaa cgggttatta aattctcccg
     4621 tttttgggag tccttgagtg ccattttttt cactttcttt tgctgtccta tccggatgag
     4681 aaggttttcc atttggtttt ccatttggtg attcatttcc tggattttct ctttctggtt
     4741 tgtcatttcc cgggggcaca tcggcagttt ctcgcacagt gatcgctttt tgctgggtaa
     4801 cagattctgt atgagcagac catttttccc catcaaatat ttccgaacga taagtgactt
     4861 ctagcgtata ttctcctttt gaaggtgctg aaaaaatagc ggtatgatta gaaaattgag
     4921 cacttgactg tgtttcaacg atttggatct tttctgggaa ataacgcata tcttcaaaga
     4981 caggcgttcc ttgatttggt gtgaaagttg acacaaaaac tgtaaaaggc ttcagttcaa
     5041 tgatatttcc ttctgcattc tccgccacac catttgcttc ttgtataata atcggattat
     5101 tttgttcatt tggttttgtc tgatttggtt cttgtacgat tgcatactct ggatattccg
     5161 aagacggtga ggtttctccg tcaaaaatag cacctacaga taatggaaga ccatccactc
     5221 tttccttttc tgtttgacga ttccccggtt taatctgcat cagtccatga acatggatac
     5281 ttccattttc atagcgcgta acaggctgct gaccttcttt cgaacatgta tttagagact
     5341 cgatccaatc ttttgatatg gtatgtccaa gagtattttc tgattgtttc tctcgttcat
     5401 tccaatagta gttcaacgag tggtagattt gatcttcgtt ttgattcaat gggcggatct
     5461 gctctctcat ctcaagattc tctgttcgga aagataagat accagaagca tggtagtctg
     5521 tgtttcctgc tgaagaggta taaagagcca cattatatga accgttatta aaacttgtac
     5581 tgctattgac gataatatct gttccgctat tggaatcgat tcctttcgca agattttcga
     5641 aagacaaact attgatcaat tcatgagggc ccgttaaaga agaaccgccc attttgaatc
     5701 cattaccgtt accttctccc tcaattcctg gtacccagcc gtttcgataa gcaactgagt
     5761 ttttgatgat tacttttccg atcgaacctg tttcattttt agcaaaaagg tcccaaccgt
     5821 catctgcatt atgatacgcg atacaaccgt caaatacatt tccttctcct attgtcagtt
     5881 tggccgcaaa tccatcggca tcttcaaatc ctggatcggc attttcaaaa gatgtacagt
     5941 tcttgactaa gttatgcgcg ggccatgctt cgaaaggatc atttgagcta ccgctgattt
     6001 ggattcctgt atttccattc cgataagcat gaatctcttc gatcaaatta tgatttccag
     6061 aaatctgtaa tcctttttgc atagctgcac tatttgtaat gtcaaaccct cgtaaacccc
     6121 agtagtgact gaacagtgtc atcccatttc cagttccttt aaaatcaagt acaggacgct
     6181 tgttttctgg atttttctct gcaatcaact gaatcgggtt ttctcttgtc ccgttaacgc
     6241 cttttggaat cgtcaatcct ctttcgaagg aataagttcc aggcgccatg acgattgtct
     6301 gcccagcata tgcgtatctc aatgcttgtg tcagagataa tggattttgt ctgcttccgt
     6361 ttcctgcatc tgacccttct ggtgttacat aaactgtcga atatttaggt ttacggtagt
     6421 ccactgtatg aagaatctct tttgtttcat aactagtcat ctctacgtat tcttctggcg
     6481 gatagttttt atctggcgta aaactgaaag taaattcgtt cgttccttcc atcagttttg
     6541 tctgataatc aaattcccga ttcgcagtga tagccaagtt ttttgcgttc ttaagcacct
     6601 ctcctgttgc tttattttta attgtcaata atccgtcagc atttgctcta aaggacatag
     6661 tatgatcttg gtttcctgaa tgattactag aggtcacagt gtaaatcgga tcaatcaagc
     6721 gagtaggtct ttcttgccca ggaggatctg tcgcaggatc tgagtaagca acttcgattc
     6781 cttctacctt gatcgtcata ttccttgctg cagcaaatcc tgcataaata accgattcat
     6841 ctgaagcgta taatttttcc cagtcataca tgatgtcaga agccatttct tctcctgttt
     6901 caggatcgta ataatatgac tctaaaccag tattcgtttt tttcatctct acgtccagtt
     6961 tagtaatcga gttcgtggcc ggaaacaaat tcccatgacc gtttccgaat atattataag
     7021 aacctgtttc tttttccaag aaccctgctg aagtctctaa aggtgttgtt tccactgaga
     7081 cactcccagg agctggcgca gcttgcggat cttcagatga tattcctgtg attgcccgtg
     7141 tccccaatcc aagtcgcatc gtatatttgc tgccgctcgt gttgaccact tcttgtctat
     7201 ccgagtccca gacatattcg atccgactgc ctagtatggc aaatgaattt gaataaaatg
     7261 cctgcccaaa caaatgattt tttggtactg cgtctcttac cattaaagca aatccttctt
     7321 gggcattcgt ataggtccaa gattctattt cgatcgtcgc ccgcatccta aagttgatgt
     7381 ctttggggat tgcttgataa taataggtca ttccatctga tccgcttgac tggaattttc
     7441 cgccgttatt cgtggaagag agccgcacgc cgtcttctaa tggttcaatg gcatacctac
     7501 tagcacttgt gctttctccg aaaacaaccc ctttccactc ttcatttgct tcttcagatt
     7561 gcttttttgt cttttcattt tctgattctt tcatcatagg atcatttcct tcttccgtat
     7621 ccatatcttc actagttctt ggtacttcct ccattgttga catcccttca atgttcacgt
     7681 gtgcattttc ttcgttcaca gactgttgtt cgttagcagc aacttcaacg acagaaatta
     7741 aaaccggggt actgagcata aggagattca tcattagcgc actgtatttt atacgttttt
     7801 ttaacaagtg tattcctccc gtaacaaatt agaaagcgtt tacttaaagt attacctaat
     7861 ttatttttac tgtcaataaa aaacaaaatt ataataagtg tattttataa aaagaaaaaa
     7921 tataacttaa gttgttgata ataaagagat ttattaacgt aaaaagaata agttttttta
     7981 aaataacgta taaaaaagag cataaaacaa agtcgtttgt tttatgctct tcttttatta
     8041 ttttgtgctt tttattttat caaatcactt tgttttttta taaaatttct gcgcctagtg
     8101 tggactcaaa atgtctcaat gcccattcat gtccaacggg atcgaaactt gccacggcat
     8161 ctttgaaaat atggagtttg tatcctagat tatacgcatc gactgctgta tgcaacacac
     8221 agatatctgt acatacgccg gtcaaataaa tatctgtgat ctggcgttct cgcaaacgga
     8281 tgtctagatc tgttccacta aaagcggaat aatgccgttt gtctatccaa taaacatttt
     8341 cctcttgctc atagtcttgg tataaacttt ttaaagagcc gtacaattcc cgtccacccg
     8401 ttcctatcac attatgcgga ggaaataagc ggttctccgg atgaaaggaa tcttttggat
     8461 catgcgcatc aatggcaaaa acgacaaaat ccttttgatc aataaacttc tttgtatgag
     8521 cagtcagctt tttttcaatt acttgtcctg ctgtcccagt agtcagcttt ccatcctctg
     8581 caacaaaatc atatgtgtag tcaatcgaga tcaatgcgtt catcttagtc ctccttatac
     8641 gagatgtttg acaaattcga tcaccatttc tgctgatcgt ttgcctgcgt cttcgataaa
     8701 ttcgtcaaag ctttgtgtcg ccgagtgatc tgctgtgtcg ctcattgcac gtacgatcaa
     8761 aaatgggata ttgaactgac gagctgtttg tccaacagcg gctccttcca tttcgcaagc
     8821 taatgcttca gggaaattcg ttaagatttc tttgatttta tctggcgagt cgacaaatgt
     8881 atcacctgta acgatcaatc cttcttttgc gttcaaattc gttgcttttg ctgctttaac
     8941 catttcactg cgcaaatatg tgcttgcttc ataatataga ggcattcctg gtaattgtcc
     9001 tggtttgtag ccaaatcctg ttgcatctgc atcaaaataa gctactttgt ctgatataac
     9061 gatgtcacct acttgtaagc cttcaccgat tccgccagct gaacctgtat tgatcaccat
     9121 gttcacgccg tattgttgga tcaacagact tgtggtaata gatgctaaga cttttcctat
     9181 accggaacgt acgacgatca cttcgtggtt acctaatgaa cctgaaataa ataaagcgcc
     9241 tgcgcgttcc catgataatg gttcgcttag gttctctctc aaaattttta cttcttcttc
     9301 cattgcgccg ataataccga ttttcatctt tttaattcct tctttctata taaatgcgat
     9361 tgctaaaaaa accacacata acattaggat aacaataata agagatttca tcaaaaaaga
     9421 gttccatttg gtcgtctttt cccgttcgct gatccttgtt ttcgtgattg gtttattctt
     9481 tttgctttct ttacgataaa agctggcaat ctttttttct tcttgctgat aagctgtttc
     9541 tgcttttcgc tgttttttta atgactcgct tgcttgtgct tgttgtcgct tgcgaagttc
     9601 gcttcgcgta ataagcggtc ctttggccat aagccgatcc ctctactttc ctttggtcaa
     9661 tagttcccag tattgaatcg cataaatcgt ctttgcatca caaatcaact gatctttcat
     9721 cgcttgttgc gcctcttcaa gagttagatg atacaactcc agtacttcgt cttcatcttg
     9781 tgctaaagga ttctcgacct tctctacccc ttgtgcatga taaatatgaa ggacttcatt
     9841 tgcaaaacct ggtgaaagat acatcgaagt aagatggctc agcgacttcg cacgatagcc
     9901 agtttcttct tctaattctc tagctgctgt catttcggga ttttgtcctt ctcctggatc
     9961 gatttttcca gctggaatct ccaaaatcac tttttctaaa ggttttcgaa attgtttgac
    10021 aagaagcaga cgatcttgtt cgtcaaaagc aatgatcccg acaccaccag gatgaaatac
    10081 cagttctctc ttcgaagttc ctccatctgg caaacgaact tcgtctacag caacatcaat
    10141 aatttttccg ctatagattt ctttgcgact gattgttttt tcttcaaatt gctcattgtt
    10201 cagcatatct tctcctgctt tctttgtaat cataactgct tttttttcat ttttcaattt
    10261 ttctcaaaaa ctccttatta gtttaccata gataaacctt agattttctc taaatataga
    10321 aaatccttct ttagtccgat actctttcta gtagtccgta tgctaaaata agtatgtaat
    10381 cgaataacga aaagaattca atactcaacg ataaatgaca tggagggttt attatgataa
    10441 aacgaaattg gccgtggctt ctagcattgg tcttgctatt tctctttgtc cgaaacaata
    10501 gtttttcttt tgttctagga cttttattga taggtgtagt tttagttttg tggggactct
    10561 ttggttcgcg caggaaaaag ggaaaacaag aagaaattcc tgcactgact gacgaacttg
    10621 aaactcacta tgaaaaaagc ggtatgacag cgaatgaaat ttcatttttt cgacaaacaa
    10681 tgaatcaaac aaaaaatgag atcaatcaat tgcaaatgaa tatgcagcag acagccaaac
    10741 ttaaagcaat cgatctccgc catgatccag tcaaagcagc gaaagcttta ttcaaagaat
    10801 tggtgaaaga accaaaccga ctgcatgaag caagtcagtt tctttatacg catctgccga
    10861 atctagttga tttgacaaat aagtataatg agatcaatga ccatgagatc aaaaacaaac
    10921 agacctatga aaaactagaa gaaagcgcgc agatcatcga ccagctgtca gctttaatcg
    10981 cacaagatta tcaaaaattc gttgctgagg atctagatga tatcgacgta gagatatctg
    11041 ttgcgaaaca gagcttaaag cgtgataata aatatgaacc agaacaaaag gattaactct
    11101 tttgttcata aaagaagact cggaacattc agatgatgtc tggctgtttc gagtactttt
    11161 tctaaagtag agacattcat gtaagaatcg agaacagatt caacaaggag gaatttttta
    11221 tggatcataa accgactgaa acaaaagatg ttacaccagt taacgataca cttgaagatt
    11281 tattgaacaa cccgttttca actccaacag atacgctgac cacttcacaa caagcggaga
    11341 ttgatcagct ccaagagcaa cagacagctg ctcgtttaat tgacaaacta ccggcggaac
    11401 gccaagaaca agctaaacag ctagcagcaa aaatcgatgc taatgatgca cagtctgtca
    11461 tcagctacgg atcggctgcc caagcaaaat taagtgaatt ctcacaatcg atgttgaatc
    11521 atgtgcaagc acaagatatt ggtcctgtag gagattcctt aactgaactg atgtatcgtc
    11581 tccaagaagc caatcctgac gaactgcgtg ccggagaagg gaacatcttc cagcgtgtct
    11641 tcgggaaggt caaacaatcg atctatgaag tgacagcaaa atatcaaaag attggtgcac
    11701 aaattgacaa gatttcagtg aaattagata aagaaaaaga cggtctgcta aaagataatc
    11761 tgatgttaga acagctttac cagaaaaaca aagactactt tgatgcactg aatatttata
    11821 tcgctgctgg agaattgaaa atggaagaac ttcaaacaac gatcattcca gaagcgatga
    11881 aaagagcaga agaaactggc gatcagatgg atgttcagat tgccaatgat tacacgcaat
    11941 tcctagatcg tctggataaa cgtacgcatg atcttcgctt agctagacag atcacgatcc
    12001 agcaagcacc acaaatccgt ttgatccaaa acacaaatca ggccttggct gaaaagatcc
    12061 aagcatctat cgctacagcc attccgctgt ggaaaaacca agtcgtgatc gcattgacct
    12121 tgcttcgtca aaaagatgca gtaactgctc aacgccaagt ctctgaaaca acaaacgact
    12181 tattgaagaa aaattcagaa atgctgaaaa tctcagccat cgaaacagcg aaagaaaatg
    12241 aacgtggaat cgtggatatc gagacccttc aaaccactca aaatgatttg atcgaaacaa
    12301 tccaagagac attgcgtatc caaaaagaag gaaaagaaaa acgtcgtcat gcagaagtag
    12361 agcttggaca catggaagaa gatctgaaac aaaaactgtt ggatttaacc caatagatca
    12421 cctttcgctt gttactaaaa caaacgccca acaacagttt attacaactg ttgatgggcg
    12481 tttttcttat ctttcttcta acttctccat tggagtctat ttctcattca tcttgctgtg
    12541 tccatagcca tagccaaaat aaatgatcag tccaataacc attacgatac cgaacacgat
    12601 ccatgtcaca gtttgcagac gaagcatcaa tacaatacaa gtcaagatgg agacgattgg
    12661 caatagcgga acgaatggca ctttgaattc tccagctttt ggtttgccaa acatttttct
    12721 caacttcagt aagcctagac taacaagcat caaataggcg agcgccatga tattcgttag
    12781 ttccgctaat acattcaatg ggaaaaatcc ggcaaagaat acagctgcca caccagcggc
    12841 ataagtcgct tttttcggtg tacgatgctt ttgatcgatt tctgtcataa acttaggaag
    12901 taagccatcc ttgctgacag caaacagtaa acgagcaagt gaatacatca tcgagatcgt
    12961 taccgtcagt agcgttaaaa ttgcccctac agcgatcacc gcgcccactg ttccatgtcc
    13021 tacatacctc attgcaaaag ccactgggtc attgactcct agttcagtat acggaacgat
    13081 tcctgtcaag atcagcgtca cgatgatgta aaggacagta gcaatcagta tcgaaccaat
    13141 gattcctctt ggcacatctt tttgcgggtt tttgacttcc tcggctgcca ttgcaacggc
    13201 atcaaatccc aagaaagcaa aaaacacaag agctgcacca tctagcacac cggaaaaacc
    13261 aaaaggcatg aatggctgcc agttatcagg tttaacgaaa aatattccta caatgataaa
    13321 caacgcgatg ataccaaatt tgacatatac catggcatta ttcaaacgta atgctttctt
    13381 ggcttcaagc gacacgatgt aagtgacgaa tatcactacg agcaccgcaa tcaagtcaat
    13441 gtatgtacca ttttccggat tgtacccgcc agttaatgct tgtggaagtt cggcccctaa
    13501 acttttcaaa aaaccttgca tatagccaga ccatccggaa gcaacagagg aaaccgctag
    13561 taaaaattcc ccgatcaaca accaccctgt cagccaggca gcaaattcac caaatacaac
    13621 atacagatag gcatatggcc caccaatcac tggcactcgc gaagcaaatt ctgcgaaact
    13681 tagtccagac aaaatgacaa cgattgcagc taagacaaat gaaagtgaca gtgctggacc
    13741 agcattttga ttggccgcaa cccctgtcac cacaaatatt cccgtaccga tgatcgcacc
    13801 aattccaagc atgatcaaat cagacgtttt tagttctttt ttcatctcac tcgtatgatt
    13861 gagattgatc tctttcttac gaaacaaatc cattgaagtt cctccccatt ttaggttgga
    13921 aaacagcgat taaactgttt tcatataccc taaaaggata ccacctaaaa tgactaaaag
    13981 gcaaccaaca ataacataaa tgagctcttt tttcgttttt ctttcgccaa ggatgaaaat
    14041 cccacctaaa gtagagataa tgatccccat ttgtgaaagt gagaagccaa cagctaaacc
    14101 aagtgatttg actgttagaa gcatgcagat gtttcctaag ccccataata atccacagat
    14161 catgtttttc cacacaaact gatcgacttt aactttacgg aaagcaaaga agcttgcgcc
    14221 taagatcatt ccgatacttt gaggtaagat aattgacatc gcatccaaat tcgcccagtt
    14281 gacgatgatg gtatatacac cataaccaat cgtagagaag atcaatgctc taaaaccctt
    14341 accaaaatca agcattttat tatctgtatc tatttttcct tcgtcatctt gtcgggcagt
    14401 caaatacgca ccgcttacaa gaagagctaa agcaataata ccgtatacat aatctttcgt
    14461 ttgtgtccat tcatggaaga aaacggctcc cgcaatcgta ttcaaaatta attgaaaacc
    14521 agtagacaat ggtagcccta cagaaacacc catgtacttc attccatgga actgtccatt
    14581 ttgtccaaca ctccaagcta agccggataa aaatccgatc aagaaaatcc aaggggtgat
    14641 tacaggacta acaacgaaaa agacaactaa agaaaacaga aatgcaccga tcgtcattcc
    14701 tagtgtttgt tgattggcat cgccaccaat tttaccactt actaaaccaa tactacccca
    14761 tgccaacata gggactaatg ctattaaaat ttccataacc aatctccttt tatttcacat
    14821 gataagaaac ttatcagcga aaacgttaac aacacaagca ctttttttcg tttctaacga
    14881 ttttctaacg tttttcatta taaaaattta agattgggaa aaataaagtc attgttgtcc
    14941 cttttccttc agttgattca gccgtcaaat ggatacctaa tttttcggcc agattttttg
    15001 ctaaataaag tcctaatcct gttgaatgtg ttttgctaag tcgaccattt ctcccagtaa
    15061 atcccttatc aaagatccgt ttcaagtctt ccgctgggat accgatccct gaatctttaa
    15121 ctgaaaggct gactccttgc gcttcctttt ctatgctcac aatgatgttt ccgtgatccg
    15181 gcgtatattt gatggcatta ctaagcaatt ggttgaagat aaaagcgatc catttagcat
    15241 cggtcaaaac agtatgatcc tcaccaacga tctcgtagcg aatatttttt tggataaaat
    15301 aatttgcctg actgcgtatc acaggctgaa tgattccttt tagtccatat tcttggatga
    15361 ggtagtcccg cgagaaactg tcgagtcttg cataatacaa aacttgttct acgtattcat
    15421 cgatttttga taattcattt tctagtaatg tatacttttg atcgtcaata tcgaactcga
    15481 tcgaacgaac tagcaattta gaagcggaaa gtggcacttt gatttcatgg acccatgagt
    15541 caatataatc tttttggtct tgttgattac tgattgctga ttgcattact tcttggtgtt
    15601 cacggatcag ctgattgata tattcttcta ccagtttttc ttcttctgtc ctcccaccac
    15661 tcaaataatt ttgcaaaggg gaagtttttt gtatgtcggc taattttccc caccatcttc
    15721 tcttgctcaa gtaaccaccc agcagataaa aaatcagaaa gacgccctca atcaaagcta
    15781 aataagcaat tgtgctcaaa tcaactttga tgtttggcgc cagccacatc acaaagatcg
    15841 tcaatgcaat aaaaaacagc catcccaaga tcaatgtcca ctgatctttc aaatatttaa
    15901 aaaaattcat cttaaccgct catcctttca gggaacgata tatccctgtc ctacttttgt
    15961 ttcgatatat ccttggatac ctgcctgttc gatttttcgg cgtaaccgat tgatgttgac
    16021 tgtcaatgta ttgtcatcta caaatcgttc gtcatcccac agtgctcgca gcaatttttc
    16081 ccgacttaag atctttccat tgtgcttcat caaaataaat aatagacgat attcattttt
    16141 gctcaaatca atgatctctc cgttgatttc catacttccg ttgtcaacat tcaacgtaat
    16201 gccattgtgg ctcatcattt cactagataa ttctgtgtag ttgtaagaac ggcgaagtaa
    16261 tgcattgatt tttgcaatca gtacatcgat ttggaaatgt tttgtcacaa aatcatctgc
    16321 ccccatgttc atagccatga tcatatccat attcgtattc ctgctggaga taaaaatgat
    16381 cggtactttc gatacttcac gtattttttg acaccaataa tagccatcca aaacgggaag
    16441 attaatatct agtaatacaa gctgtggttc ttctctttca aagtctgaga acacttgatt
    16501 gaaatcggtc gttccaaagg tttcaaattg ccatttctca agttcctcca tgatcaattg
    16561 tcgaatgatc tcttcgtctt cgactaccat gatttttgcc ataaaaaatc cctccatctt
    16621 tcttcttgct actacaatat caaagaagcc cttgtgaaac aagttggctt acctggaaaa
    16681 tgtcatttta gcaaacgtta aaaaaaactg gaaatatgcg atttaatcgc gtttgtctct
    16741 tccaagcagc ttaaatttat tatgttattc ttaataattg gtctattatc tgttattttt
    16801 ctttcgttgt ttttgtcttt taaattttcg ataacaccaa taaccgaaaa gcgttagcac
    16861 tagcaatata cctagaagga gtataagtaa tcgataaaga tggtattttt ttgtctgttc
    16921 tatctttgaa gagacttctt ctgtaaaggg aactcgtctt cctgtcacta gtaatcggtg
    16981 agtgttcacg gtgtaaggtg tacaagtgat aagtgtgact agatcctgct tttctctaat
    17041 tgctaatgta tttgtttttg ttggaaggac aacaattttc tcaacgactt cataagcgag
    17101 catccgacca tacacttcaa gatagaatcg gtcatcttgc tccatttctt caagatccgt
    17161 aaagagtttt ttctcagcaa taccgctatg ggcggtaatc acggaatgtg tactctctcc
    17221 tccagttgga aaagaggtgc cttgcaggac tgtcgctcct ctatctaaca aaagatcatt
    17281 cgtttcatca aataaaggca agctaacatt gattttcgga atatagatgg ctccgatcat
    17341 atgttgttcg taatattctt ttcctggatt ttggacattt ctaaccgctt gctcaaaagg
    17401 atctttgact tgtcccattc ctgggattac tgatgtatga gcaagcgtct gatttttctt
    17461 ttcatgcact gctaattgtt gctcatgttt ttcttcttta tcgcgttcaa gctgcttttg
    17521 ataattctcg atccttcttt gatccaaata attgctgaga gcatctgcta aaaatggata
    17581 tgtaaaaact gcagctccgg caagaaacat cactgccata aataattgta ataaaagact
    17641 gatttgggtt tgaggtctat tacctatttt ctttttcatt ttcttcacct tcttatttaa
    17701 aaaaactgca gcaaccttta taaaaccgat tgctgcagct tatttttgtt gcttatactt
    17761 cagacttcat tttagattta cggtaccagc tgtacgctcc aatcatcaaa cttattccga
    17821 ttagtaagaa tgccaagagc ccgttcccac cagtagcagg aagtgacccg cgtttcatat
    17881 tcggtactct ttctggccct ggattttcta ctcctacgat ctgaatttct tcattatagc
    17941 ttccataaga gatgataaat tcagtaaagg ctgcctcacc tgttggaagt acataacctt
    18001 ctggcgccat ggtctctttc atttgatagg ttccataatc taacccttga acctcaaagt
    18061 atccatttcc atctgcgaca aattctgttg cttgtccgct tgttgcccac tcaattctat
    18121 ctgctcctga ttcataaaca gctaaaggcg actccccttt ataaaatatt gcgtattcag
    18181 aacggtcttc attccacaat tcgaatcttg catctttaag caattcactg cttgatgcat
    18241 catatttttc aaactttctt ccacctgttg tgactttctc tggcggctct actccttctt
    18301 cataatcaaa ttcatcattt cgtccgattt caataaccgc tgtgttgtta tggaaatcat
    18361 ccgcttctag ttctgtggaa accttcaaat tatacgtgat cgttaaatct cctccacgtg
    18421 catctgctaa tctacctaaa gtatctactt tggctactcc attttcttct gtcaaaatcg
    18481 tcacgacaaa cccgttgctt actacttcta ctatataatc ttgtcctttt gttaaaatcg
    18541 tctcgccggc tcgcacttct atttcctgat cataataagt tagaccaact gtaggcgtat
    18601 ctgtgatttt gtaatttttg ataacagcgc ttccgttttc aaggacagaa tcgatgaaat
    18661 aaggaatagg tgctgtgatc gtgtaagaag ccgtttttcc tctttctaga tcaatataag
    18721 aaatcgtttc ttgttctcca gcttcattaa taatcgttac ttgtctaagg tcgctttcct
    18781 ctacattcat ctgtttatct gcttctctga tttcgttttt cggatacaaa tgcacatctg
    18841 tattgtatgt gtctttccat gtagaaccat cccacacgcc atccaccata tcaggcatca
    18901 tcactggcat cataacaacc attggagacg cgattctgga aatatgagct ggcgaataag
    18961 tttctaagaa aagatagact ttatctctag aagaaggtgc ttcttccgtc acttggacgg
    19021 tagagaatac cgcttctcca gcttcattcg ttgtttgttt gtcaatactt gtcagtcgat
    19081 attttaaaaa ccaactttct gtgtgccgtt tttgaatcca ttcaatcgct tcggcagaat
    19141 taagaccgtc ttctcgttcc ggtgttaaag gattatctcg aatcaaatcg taatatactt
    19201 ccgttacgtc aaacaaatca aaatcgactc ctggaatatt ttcttcacca aaatccgtag
    19261 aaaccaatcc gctattcggt cgttcttccg gaacagatga gaaacctttt ttgtgtaatg
    19321 taatcgtcac ttcacttggt ttttgtgcga cttcttcggc tttgactagt tttccgttcc
    19381 cgaaaatccc tattcccgca gtaacgagta acagtacact cattcccgtg acaatcattt
    19441 ttttattcat ttttttctcc ccgttctttt tttatt
//
LOCUS       DAJSAW010000052        18740 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJSAW010000052 DAJSAW010000000
VERSION     DAJSAW010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.052"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            256..531
                     /locus_tag="ONT64_002065"
     CDS             256..531
                     /locus_tag="ONT64_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303471.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="ONT64_002066"
     CDS             544..2616
                     /locus_tag="ONT64_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCR2303472.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2781..4667
                     /locus_tag="ONT64_002067"
     CDS             2781..4667
                     /locus_tag="ONT64_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR2303473.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="ONT64_002068"
     CDS             4679..5626
                     /locus_tag="ONT64_002068"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR2303474.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="ONT64_002069"
     CDS             5646..6173
                     /locus_tag="ONT64_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR2303475.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="ONT64_002070"
     CDS             complement(6236..6889)
                     /locus_tag="ONT64_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR2303476.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="ONT64_002071"
     CDS             7021..7863
                     /locus_tag="ONT64_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR2303477.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="ONT64_002072"
     CDS             8021..8917
                     /locus_tag="ONT64_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR2303478.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="ONT64_002073"
     CDS             8920..9633
                     /locus_tag="ONT64_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HCR2303479.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="ONT64_002074"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="ONT64_002074"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR2303480.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="ONT64_002075"
     CDS             10168..10395
                     /locus_tag="ONT64_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCR2303481.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="ONT64_002076"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="ONT64_002076"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR2303482.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11681)
                     /locus_tag="ONT64_002077"
     CDS             complement(11181..11681)
                     /locus_tag="ONT64_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCR2303483.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="ONT64_002078"
     CDS             complement(11960..12358)
                     /locus_tag="ONT64_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2303484.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="ONT64_002079"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="ONT64_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCR2303485.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="ONT64_002080"
     CDS             13471..14238
                     /locus_tag="ONT64_002080"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR2303486.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="ONT64_002081"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="ONT64_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR2303487.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="ONT64_002082"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="ONT64_002082"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR2303488.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="ONT64_002083"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="ONT64_002083"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HCR2303489.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5092 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt
//
LOCUS       DAJSAW010000053        18223 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJSAW010000053 DAJSAW010000000
VERSION     DAJSAW010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.053"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(27..725)
                     /locus_tag="ONT64_002084"
     CDS             complement(27..725)
                     /locus_tag="ONT64_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCR2303490.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="ONT64_002085"
     CDS             complement(718..2070)
                     /locus_tag="ONT64_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HCR2303491.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="ONT64_002086"
     CDS             complement(2293..3693)
                     /locus_tag="ONT64_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCR2303492.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="ONT64_002087"
     CDS             3854..4591
                     /locus_tag="ONT64_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR2303493.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="ONT64_002088"
     CDS             4716..5195
                     /locus_tag="ONT64_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HCR2303494.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="ONT64_002089"
     CDS             5192..5959
                     /locus_tag="ONT64_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303495.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="ONT64_002090"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="ONT64_002090"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR2303496.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="ONT64_002091"
     CDS             complement(6862..7464)
                     /locus_tag="ONT64_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2303497.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="ONT64_002092"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="ONT64_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="ONT64_002093"
     CDS             8145..8729
                     /locus_tag="ONT64_002093"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR2303498.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="ONT64_002094"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="ONT64_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR2303499.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="ONT64_002095"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="ONT64_002095"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR2303500.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="ONT64_002096"
     CDS             10630..10809
                     /locus_tag="ONT64_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303501.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="ONT64_002097"
     CDS             10831..11856
                     /locus_tag="ONT64_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR2303502.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="ONT64_002098"
     CDS             12031..13275
                     /locus_tag="ONT64_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR2303503.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="ONT64_002099"
     CDS             13428..14375
                     /locus_tag="ONT64_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2303504.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="ONT64_002100"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="ONT64_002100"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR2303505.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="ONT64_002101"
     CDS             complement(15277..15519)
                     /locus_tag="ONT64_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303506.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="ONT64_002102"
     tRNA            15640..15712
                     /locus_tag="ONT64_002102"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
     gene            complement(15831..16469)
                     /locus_tag="ONT64_002103"
     CDS             complement(15831..16469)
                     /locus_tag="ONT64_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR2303507.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16592..17224)
                     /locus_tag="ONT64_002104"
     CDS             complement(16592..17224)
                     /locus_tag="ONT64_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR2303508.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17575..17874
                     /locus_tag="ONT64_002105"
     CDS             17575..17874
                     /locus_tag="ONT64_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR2303509.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6264 a         3169 c         3585 g         5205 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    15781 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    15841 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    15901 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    15961 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    16021 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    16081 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    16141 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    16201 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    16261 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    16321 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    16381 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    16441 tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca gtttttgaag
    16501 atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga tatttgtatt
    16561 gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt aaaaattttc
    16621 tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt tccgagcgta
    16681 cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca gtatcaaaaa
    16741 agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa cagtctacaa
    16801 aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt gaaccagata
    16861 aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa ctttccatca
    16921 atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg ctttttgtca
    16981 atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc attctttgaa
    17041 aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt ggatggcaga
    17101 ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca aaatcttcgt
    17161 tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact ttaggaaaaa
    17221 tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa ccaacttaca
    17281 ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg ctatctcttg
    17341 aaacgttctt agaattgccc tgagagacac aaattaggcg taattctaat aaaaatctta
    17401 gaataaaaat ttttactgat ttaatttaga atgtttttaa aataaaaatc gttgccataa
    17461 cagtgtattg aaaggtttta acaatccagt aatgatttaa aaaaatctag atgtagcgta
    17521 tactttgttt atagaatgta gtgggaagaa atatattttt gggaggaaat cgtgatgtct
    17581 aaaagtgaag aaatagaaaa catggtacta aaattatatg atctcgtttt aaatccagag
    17641 atcaaagaaa aggaacgtat acttttgata gacgcaaaga caggcttaga aaaaggtcag
    17701 tattatccta aagtcataaa taatttggaa cgtagtttac gtccgttagc aatcagggga
    17761 gaattgtcta aaccagtatc accgttttat atggagatat caacaatagg aaaatttgaa
    17821 aaagaattag gtcgaggaat ggcttcggca ccgattactt ttgggcattt ataaaaacga
    17881 gttattcatc tcaatgaatt aaaaagtata gtcaatctca taatgcaggc taggttcatt
    17941 tatttgaaca gagtctgtat ttttttattc taaataaaga aacagatgtt tctttatatt
    18001 gtattttatc ctttaattaa cacaaaaaca gctagtataa actaataatg ttaattaaaa
    18061 gtgtatttta tgaaactatc gttatttatt aatgttgcat ttttgtaagt taaaaattgg
    18121 aaatatagat tgaaaaaatg aaagcaataa gtaaaataaa atgaatatat gcgaaaaaat
    18181 aatttacatt aggaaaaacc ttgtaaaaac agttttttgg cat
//
LOCUS       DAJSAW010000054        17501 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJSAW010000054 DAJSAW010000000
VERSION     DAJSAW010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.054"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1020
                     /locus_tag="ONT64_002106"
     CDS             <1..1020
                     /locus_tag="ONT64_002106"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303510.1"
                     /translation="VFLPQITEYLNYRIFTTKVDDSVTSLSRLEFIKICASVFGPILT
                     VMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKELLTQIDREFYFLLNMFIEIQKD
                     QNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEIGSTIEQSGPLKGTVEYIKFET
                     FDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQIYLGRYFKMFHRIVKTLNEYY
                     DDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYIKRGFGLGIQLIGSGFFGDDFD
                     FETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYIKELGSVEYKKIANFESLYKLF
                     NETKL"
     gene            1064..2236
                     /locus_tag="ONT64_002107"
     CDS             1064..2236
                     /locus_tag="ONT64_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR2303511.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            2223..2927
                     /locus_tag="ONT64_002108"
     CDS             2223..2927
                     /locus_tag="ONT64_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCR2303512.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            2932..4092
                     /locus_tag="ONT64_002109"
     CDS             2932..4092
                     /locus_tag="ONT64_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR2303513.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            4168..4446
                     /locus_tag="ONT64_002110"
     CDS             4168..4446
                     /locus_tag="ONT64_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR2303514.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            4427..4798
                     /locus_tag="ONT64_002111"
     CDS             4427..4798
                     /locus_tag="ONT64_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCR2303515.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            4814..5218
                     /locus_tag="ONT64_002112"
     CDS             4814..5218
                     /locus_tag="ONT64_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303516.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            5215..5625
                     /locus_tag="ONT64_002113"
     CDS             5215..5625
                     /locus_tag="ONT64_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303517.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            5686..6273
                     /locus_tag="ONT64_002114"
     CDS             5686..6273
                     /locus_tag="ONT64_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR2303518.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            6346..6813
                     /locus_tag="ONT64_002115"
     CDS             6346..6813
                     /locus_tag="ONT64_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HCR2303519.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            6859..7167
                     /locus_tag="ONT64_002116"
     CDS             6859..7167
                     /locus_tag="ONT64_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303520.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            7362..14096
                     /locus_tag="ONT64_002117"
     CDS             7362..14096
                     /locus_tag="ONT64_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR2303521.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            14100..14972
                     /locus_tag="ONT64_002118"
     CDS             14100..14972
                     /locus_tag="ONT64_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HCR2303522.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            14969..16018
                     /locus_tag="ONT64_002119"
     CDS             14969..16018
                     /locus_tag="ONT64_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303523.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            16022..16846
                     /locus_tag="ONT64_002120"
     CDS             16022..16846
                     /locus_tag="ONT64_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303524.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            16864..>17501
                     /locus_tag="ONT64_002121"
     CDS             16864..>17501
                     /locus_tag="ONT64_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HCR2303525.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         6287 a         2659 c         3704 g         4851 t
ORIGIN      
        1 gtatttttac cacaaattac tgagtattta aattatcgga tatttaccac aaaagtagat
       61 gatagcgtta ctagtttaag taggctagaa tttataaaaa tatgtgcatc tgtgtttggt
      121 cctattttga ctgtaatggt atttgtgaat actttaaata tgcagaaaaa aacagagcaa
      181 aagcaaaatg agcttgaagt aaagcaagct aaggctgaga acgagagagc aaaaaaagag
      241 ctgttaacac agatagatcg tgagttctat tttttattaa atatgtttat tgaaatacaa
      301 aaagatcaaa atgccaaaca agctgttatt gctttacaga agttggcaat ttttaacgaa
      361 aagggtttta atcaagacta tgcatttact tctaaagatt tatcagatag tgaaattggg
      421 agtacaattg aacaatcagg accgctgaag ggcacagttg aatatataaa atttgaaaca
      481 tttgatgtaa gcaagtcgat ggggaattat caaacaaaaa caaaagacgt tgctacaagt
      541 ttacacgatc agaaagtagc tatattgaat gaacaatatg aaaagatgca aatatatctt
      601 ggacggtact tcaaaatgtt tcaccgaata gttaaaacat taaatgagta ttatgacgat
      661 tataatgatt ttgatgtgaa gaggtataca aaatatatag ggacattaag aacacaaatt
      721 tctccagctg aattccaagt gattttattc aattctctat atataaaacg aggttttggc
      781 ttaggaattc aacttattgg aagtggtttt tttggagatg attttgattt tgaaactaat
      841 caacattttg aaacaagtat caatgaacag tggtttctat cactatcaac tgttgacagt
      901 gataactcta taaagcgaca aaaattatct gaatatatta aagaattagg atccgtagaa
      961 tataaaaaaa tagctaattt tgaaagttta tataaactct ttaatgaaac taagctctag
     1021 aaatagggct tttttatttt tctttgaaag gtggtgaaaa catgtgagct tatttgatat
     1081 ctttaagcag tcaataagaa atgaagagcc ttctgagtgg ataccggatt ttgtatatgg
     1141 agaggaagta tctgctcggg catacctcaa aattatggca aaaaatactg ttttagattt
     1201 tgttgcaaga actatgtcaa cgttagaagt taagtttaaa aataaagatg ggacatcaga
     1261 ctgggattat attctaaatg ttcgacctaa ttctgatatg tcagcagcta cattttggca
     1321 aaaatttttt tatcggctct tagatgacaa cgaagtgtta gtaatattta cagatgataa
     1381 tcaattgctg attgctgatg attttagtcg aacggagtat gccgtgtatg atgatgtttt
     1441 tacaaatgtg atggtaaaaa attttgtctt tgaacgttca tggaacatgt ctgacgtcat
     1501 ttatatggaa tataataacg aagaattaga aaggtttaca cgaggtttat ttgaagatta
     1561 cgccgcgttg ttcggacgga ttctagaagt tgcaatgcgt aacaaccaaa ttcgtgggtc
     1621 tgtatccatt gattcaacgg gaacggcgaa tgatgaaaaa ggaaaagatg gtaagactag
     1681 atctcaaaga ttacaagaat atattgatag gatttggaac tcttttaaaa ctaactcagt
     1741 tgcaattgtt cccaaagtga aaggctttga gtatgaggaa tacaccaata aacaaggcgt
     1801 atcgaatcaa tcgctagatg aactgaataa gatgaagtcg tctttaattg atgatgttgc
     1861 caacgccata ggagtaccta cggcgcttat ttatggtgaa aaagctgaac tggattcaaa
     1921 tattaaagct tttcgtaaat tgtgtattgc tccactagtt aaaaaacttc aagacgaatt
     1981 ggtggcaaaa ataattagtc gtcaagagta caaaaacggg gaacgtatag aagttaaaaa
     2041 agttctccaa aaagatctat tagagtatgc tactcaaatt gataaaataa catctggtgg
     2101 tgcgttttat attgacgaag ttcgtgatga agttgattat gatgagttgc caaataatga
     2161 aggaaaaatt cgtattcgta cgaaaaacta tgaagaaact gtgaagggag gtgagaacga
     2221 caatgccgaa agctaaaaaa gtaccatttc agtttactaa cgaaattcag aatggtaagc
     2281 acattctcac cttgagtgga aatgtacaga aaaaatattg gcgtgatgat gatgtcataa
     2341 acgctaaaga tattcgtgac tctctagata ctgtaaccga tgatattgta atcaagttga
     2401 acagcccagg cggtgatgtg tttgaaggga ttgagattta caattattta aaagatcacc
     2461 catcaaatat tactgtagaa gttacagggc ttgccgcaag cgcagcaacg tttattgtcg
     2521 ctggtgccaa tgatgtgatt atgaatgttg ggacttcttt gatgattcat gaagcttcta
     2581 catttgcttg ggggaataaa caggacatcc aaaaaacgtt aaacgcattg gaaacgatcg
     2641 atgattctat cttagctatt tattcagata agactggcca atctgttgat aagttgcgtg
     2701 aatggatgaa tgaagaaaaa tggtttacag cagatgaagc tgtcaaatat ggatttgcca
     2761 attctgtgaa gcgtgaagaa ccacaaaatg aacctcaaaa tattgcagcg atgattcaag
     2821 atgctgttgc cgttgcaata gcgaatttaa aggaacctgt tactaataaa atcgaacaag
     2881 aatcaaaaca aaaatcatta atcgcacgat tgcgaaaagg agaataaaga tatgttaaaa
     2941 attacagata aaactgcaga tgcaaaaaag gcatttaatg ctatttcttc gaaagaagat
     3001 gcaacacccg aacaaattaa tgctgctcta gaagaatatg taaccgccat tgcagaagat
     3061 gccggcaaac aggtgcgtgc tgaatatgaa gaattgaaaa atgtgacgga caaccgtgta
     3121 ttagaggctc gtggaattcc tacactaaca gcagaagaaa caaagtttta taatgaagcc
     3181 gtaaaatctg gaggtttcga ttctgacgta gtttggccag aaacgatttt ggaacgtgtt
     3241 tttgaaaatc ttcaaaacga tcatcctatt ttaggcctca ttaactttac accaactgta
     3301 ggtcgtgtca aagtaattag agcacgtcgt aaaggtgttg ctgtttttgg accattgcat
     3361 aaagaccttg aaggccaact agatgctgaa tttggtgcaa ctgaatttgt tcaacttgcg
     3421 ttgaccgcat ttttcttaat ttctaatgat accttagatt taggaccacg atggattgac
     3481 cgttttatca atctttcttt aagtgaagca gtacgtgatg tttgggctaa aaaaattatt
     3541 acaggttctg gtaaagatga gccaattgga ttgcttaaag atctagatgg tgcggtcaca
     3601 agtggtgtat atccagataa agctagcgca gggacattaa cttttgcgaa agagaagatt
     3661 gttcttgaac ttgcaggggt gatgaaaaaa ttatctaaat acacatacaa agtagatggt
     3721 gacgatgaag gtgaagtgaa ataccgttct gttgctggac gagtgtatct aattgttaat
     3781 cctgttgatt actgggatat cgtagctaga gttacatttg caaatttaaa caatgtgtat
     3841 gggactacga tgccatttat cgctgttgat catatcatcg aatctgtaga cgtaccagaa
     3901 agtaaatgta ttgcatacgt tgataaagag tatgaagcta ctcaatcaag agctgaaaaa
     3961 gtctatgtat ataaagaaac ttttgcaatg aaacgcgcaa ctttgtatgc tgtcgacatg
     4021 cttggtaatg gctatccaac aaataatgat gcagcacaag tatatgactt ggactttaca
     4081 gcgggggagt aatgtcccct gcaattggtg agataacacc gacgactgat ggggcagtca
     4141 ttaatctttc gtaaatgggg ttagagtatg aacactgaag aatttataaa agaatataag
     4201 gcccgatttc gtattttcca tacttccgaa gatgaggata tcgggaaaca gttagagagc
     4261 gggtttgccg atataaaatc tctaatcggc agttttgatc cttccactta tgagaaagga
     4321 aaagaattag tttatgaacg tactcggtat ctacggaacg aatcactaga gtacttttac
     4381 gacaattttc aaacaatgat tatggatgca tcaattgatc tggcaggtgc tggatatgcc
     4441 aattaaatca aattataaga agcctaaaat tgtagctggt gatctaaata cgccagttac
     4501 tttttttgag tttcaaccta ataagggtcc agaacctggt gatgaagaaa aaaaggaact
     4561 gtatttttgt acagcactag tttacaaccc atcaatgaaa gatcgtgaca ttcttggcac
     4621 aaaagaaggc gttacgataa aaatacggga tccccatcaa tcttatattc ctactaacaa
     4681 acataaagta aggatattag attatcgata tggagataaa gtttgggaaa taatcgatgt
     4741 ctcaccggat ttggaagaaa attcaattgt aaagatagta ttagaattaa ttacttaaat
     4801 ttggtgataa atgatgagta acgtaaagtt tgaaggcgtt caagaaacaa tccaagcttt
     4861 agaaaaaaag tatggagaaa agaaaactaa gacattaaca aaaaaagcaa tcaatgttgg
     4921 cgctgaaaaa gtagaaaaac agttgcaaac ggacatgacc gtttttaaag acaaaggtta
     4981 tacgatcgct gaggttgttc gcaagaacgc tacttataga gattacaagg cagaagctga
     5041 aatcggttgg aatggtccac atcaacgata tagaatcatt catttgaatg aatggggata
     5101 tacacgaaag ggcaaacaaa ttagaccgag aggatttggt gtaataacaa aatctcttaa
     5161 gaattccgag ctgttgtact ttgatgctgt aggagaggag gttaaaaaga gtttatgaaa
     5221 gatatgctta atatcattta cgaggctctt tgtttgaatg agtatattca ttcaatgacc
     5281 tacaacgaga ataccgagca atatcgtatt aaattttatg agcaacctga aaccgccgat
     5341 aaaacaggtc caatgatcac aattcgtcct gtagatacac ctaacgaagc gtaccacgga
     5401 agtaacaaag agctttctgt ggagcatatg atacagattg acgttgaatc gaaatataga
     5461 gcaacatgca agcagattca atatgagata aaaaaagaga tgaaaaaact aggctttggc
     5521 caaatttccg gtcaagggtt agatgaatac tttactgaaa caaaccgctt tgtagacgcg
     5581 cgtcgttacg aaggaaatac aaaaatttac gataccgaat attagaaatt aagacacgaa
     5641 aataacgtgt ctttttttat tacccaaaat taggaggaaa attttatgac tttagtagga
     5701 ttcaaaaaaa tgacaatcgg ggtctttgac agcacaggga agattccagc agcaaattta
     5761 tatgtaattg aaggtgaaca aaacaaaggg gcgactgtat ctgctgaaat cagtgggcta
     5821 tcaaaagaac catcaaaagt ttacggatcg aacatcgctt actatgtctc tcaaaaagga
     5881 acaggcgatg tttcagctac ttttggattg ttagatttac caacagaagt aaatgacaaa
     5941 attcttggtt acaaaaccga tacaaacaaa atcagcttct tgggagaaga tactgagcca
     6001 ccatattgtg caattttgat ggaatcggag gatttgaacg gcgatacagc aatgcttgcg
     6061 atgttcaaag gaaaattcag tcgtgaatct attaatctta acactacgac aaatgaagct
     6121 ttcgaaccag aagcagaaga atatgtattt tcagcgattg ccaatgatgt tgaaggtgat
     6181 gctaaaggcc aaacagtcgc aaaatatgtt ggagacgaag aagcttctat tactggtttg
     6241 aaagagatgg tatttcctgc agcgggggag taactagccc cgttgttgga gcaatcactc
     6301 ccactattaa tggggcaaca gttgaattaa gttaggagga taactatgcc agatacattt
     6361 aaaatctata aaaaaaatgg tactgaattt acaaaagtcg cagagggtga aagtccactt
     6421 tctattactg gaattgcagc aaatactcaa gttgctaaag gagattatca agctacgcgt
     6481 gtggtcggag gtcaagaatc agtaaaagta gatattcctg cattcaccac tcttccaatt
     6541 tcagttactg gtgtgacgtt ggataaaacg acagctgaag ttgaacaagg cggaacctta
     6601 aaattaactc ctactgtaac accagcaaat gcaacagaca aaacaggaag ctggagcagt
     6661 tcgaatacag ctgtagctac ggttactgga ggcaacgtta cagtaaaaac agacgctgaa
     6721 gttggcggaa ctacagaagt ttcatttact actacggatg gcggtaaagt agcaaaatgt
     6781 actattacag ttattgaaaa agcagcgggc tagtcttcgg actagctttt ttatttggaa
     6841 aataatggag gaatctaaat ggcaaaagta agaattgaat tgaaagaaga atcaggaaag
     6901 aaagtctatg aaaacataga cacaacaggg aaagattatc gaaaagcttt agaaacaatc
     6961 aaaaagctaa atgaagacgg agtaatgatt tggaatcgtt tagacattta tttggatttt
     7021 gctgtaggta tcttcaggga tagcaaattg acagctgaac aaattcttga tggcttacca
     7081 tctgagaagg taatgagcac attggacgac atcctaggag aagtaatggg gattgaaagt
     7141 aatccagacc cagacgcaaa aaagtaacac ccgaagaagc agaagaaatg tacttaaacc
     7201 tatgcagaga gttagtgaaa gcgggatggt cactatctga catagaagat aattcctttg
     7261 aaatgatgat gaaaattgcg tgcacttcgc ctaagaaaga gaaacaaaaa gaagttgatc
     7321 ttaaagactt cttgaaatcc atgtagagag gaggtaaaca tatggcaaat ggaaaacctt
     7381 taggcaatat gattatcaaa ctagacttag acagttcagc tttttcaaaa ggtctagccg
     7441 gagctaaaaa cgctgttaac catcaaatga aagcaatgaa atcacagatg caagtaatga
     7501 actcttcagg aaattctctc ggtgctttac aagcaaaata caatggttta ggcggagttc
     7561 tacaagcaaa tgaaaagcaa gtagaattgc tcactaaggc ttataaggat agttttgact
     7621 ctaacggaaa cgctactgct tcgactgcta aatacgctaa tcaattgaat caagcaacag
     7681 ctagatcagc aagttacgaa tctcagatga aaacaactgt tggacagatt gctcggatga
     7741 aagtggaaac ggaagggttt acggggaaac taaaggctaa ttctgatgta ttgattaatt
     7801 ctgggaaaaa aattgaagcc tttggtaaag gagtttctgg gataggatct gccctaactg
     7861 ttggagtaac agctccaata gtggcaggcg caactgctgt tactagtgca gctatttcat
     7921 gggaaagtgc ttttgcaggt gtaaaaaaga caaatgatga agttgtagac tcgaatggaa
     7981 acgtagttta ttcctatagc gacttagaga aaggtctgcg taatcttgcc acccaattac
     8041 ctggtagcca tcaagaaata gcaaatgtag ccgaagcagc cgggcaatta ggtataaaga
     8101 ctgaaaacgt agttagcttc actaaaacga tgatagacat gggtgaatcg actaatatgt
     8161 cggctgaaac tgcggcaact tctttagcac gatttgctaa tattacccaa atgtctcaaa
     8221 aagactttga taaattagga tcagtcattg ttgatctagg taacaatttt gctacaaccg
     8281 aatcagaaat cactgaaatg ggattgcggt tagcgggagc tggtaaacag attggaatga
     8341 gccaaggcga aattctagga tttgctactg ctctatcttc tgttggtgtc gaagctgaag
     8401 cgggtgggtc tgccttttcg aaggtcatga tccagatgca actagctgta gaaaaaggat
     8461 ttggcgcttt tgatcagctg aaacagatgg cagaagaaca aggtgttccg tgggttaatt
     8521 tagtaaatgc tgtgagagac ggtggaaaat cgttaaaagc agtatcagag caaatgggat
     8581 tcacttcatc tgatttaaag aaaatgtata aagaagcgga caattcgaaa agttcgcttg
     8641 aaaattttgc gaatgtggca ggtgttacaa gtgaacaatt ccagaagttg ttcaagagtg
     8701 atccttcaga agcaatcatc aagttcattc aagggctgaa agattcagaa aaacatggta
     8761 cttcagcaat caaagttctg gatgacatgg atataaaaga agttcgacta cgcgacagtt
     8821 tattgagagc tgcaaatgct agtgacatat tcaaaggagc aattgaaaaa ggaaacggcg
     8881 cgtggaaaca aaatacagct ttaacaaatg aagctaataa aagatacgaa acaactgaat
     8941 caaagctaaa gatattgaaa aacgaagtga ttgatacagc aatcgatctt ggaggtccat
     9001 ttgtagacgc gttgcgtgat ggtctaaatg tatcgaaacc attgataaaa aacttaggga
     9061 atatggcgaa agctttctca gctatgagtg atgatcaaca aagaaacatt atcaaatggg
     9121 ttggattagc agcagcagct ggtccagcgc tgaagctttt aggaactggt acttcagtaa
     9181 taggtaaagt tacttcaact actggaagat tgacgaaaag ccttgttgat ttagcagcga
     9241 aagcagcaga aaagaaggct atggatgcct ttgcatcgtc ggtaacaaca gctggtgctg
     9301 cagctgcaaa gacagcagga gctggaggta ttggcgggtt agtccctatg atgggaaaaa
     9361 cagctagtgc tgcaagtact gctgcgggcg ccagcggaat aggcgcaatg acaacgtcac
     9421 taggtttgtt aggtcctgct ctacttggta ttgttggcgt tggtggcgca ctggcagttg
     9481 gctatggagc atggaaatta tttggtgaag aagcttggaa ttcctcgcaa agagtaaagc
     9541 aatggggaac agatgtcggt gaggagatag atagtactct tgacggagta cagaagaata
     9601 ttgaaggtgc aaatggtcaa ttcggtcttc tcaaagaggg ttttactgtt gatgatgcaa
     9661 ataatatggc ggctaatttc gcaaccgctg gaacatcttt ggaaacatct ttaacgggta
     9721 gaataaaggc attagacgat gcactaaaag ggctacctca atcagttcag gatgccatga
     9781 aagaagtagc tgaatcagaa gaagaaacct tagggcaatc tctttctaaa ataaaagaca
     9841 acaatgaaga aataaaaaaa atcagagaaa gagccgcaaa tcagaacaga gacttgactg
     9901 tttcagaact acaacagata caggatctaa tgaaaggaac ttctcaagcg tatgtggata
     9961 cgctcgatgt ttctgctaaa gacagaaaaa agatactatc agccatgaat ggtgatgttg
    10021 cacaagctac agaagaagaa gcgaagacgt ggttacaatc tttagcgaaa caaaaagctg
    10081 caactaccga aaattataga caaatgcttg aagaaaaaaa ggcggcttta aaggaaaagg
    10141 gagtaagcaa agaattcatt gatgcattag aagatgaaat gaatgattac atcaaaacta
    10201 caaatcaagg attcgatagc caaatagctg ctataacaaa taaatatcca caattgatta
    10261 acgaagtgag tttatcaaat ggtcaattat ttagcaaaac ggcagctgct atggacaata
    10321 ctggtcagta tacaaaagtt tttctcgaaa gtaatgaaaa gattttagcc caagcgaaga
    10381 aaacatcgga tgaaatagca gataatgccg aaaaaaatgc agaacaactt ggtatggtag
    10441 ctgatgaggc tacgcatgct ggatcaactt ggaatggctt agttttagac gaaaaaacag
    10501 gaaaagtaaa aactaatgta caagaagtag tgaatgaagc aacgaaggat ttcactaaat
    10561 ggaatgaatt gaaacctgtt gttcatgatg cgaaactaac aacgaatgca aaagatgtca
    10621 tttccatagc ggcagtacaa aatggctatt gggatgatat ggaatggaaa gaaaaagaag
    10681 cattattaga agatgagtgt tctaaaaatg tatacaaggc tttagaaaat gctggtaaat
    10741 ggaatgagtt agatattcct tctaaagaag caatcctaac ttcaaatact cctgaagtta
    10801 tgggtgaaac tctcatgaat ttgggattat gggatactta caaacttcaa gtgaaagatt
    10861 tagatttgaa tgcgtataaa gtatacgatg ctatcgatgg atcagaagaa aaattaaata
    10921 gttgggctca attagataac cccacaaaag aattgttatt agataactca gatttttcta
    10981 caaaactttt taattccgaa gaaatgttta aacgttggaa tcagttatca tctaatgcaa
    11041 agtttctttc ggctaataat actaaattta cagaggttct tttatcatct gacagaatgt
    11101 ggaatcaatg ggtaaacctt cctgataatg agaaaagatt acttgcagat aacgaagatt
    11161 tagctaccac tgttttatct tcagaagaaa tatacggaag atggactcaa ttaccagata
    11221 atcaaaaata tatgttagct gacaatactg acttagcaaa caaaatattc gcttcagaag
    11281 aatcatggaa tgcatggaaa gcaattccag acacagttaa aaacttgctt ggtaataata
    11341 ttgatttact ggctaaagtg aaagacggga ctgtaagtgt agaagaatat aacaaagtta
    11401 tgccacttct taaaactcta tatggagata acgttaattt aaaagaggca atcagtgggg
    11461 ctaaaagctc tatagagaat tacaacaaaa atatttttcc tagtgataaa actgcagtag
    11521 gacacgatga ggcttcacaa gctgctaaag atgcatttga agcgttcaac atcttccaaa
    11581 caaaaatacc agataaaatt actaaaactg tatcagctga ctttataggt cctatgccga
    11641 aaaatgcgaa aggaacaaac ttccatcctg gcggagcagc tatggtaaac gatcaaaaag
    11701 ggcctactta tgaagagctt ataaccttgc caagcggaga aacttttata cctaaaggac
    11761 gtaatgtcgt tttagactta ccaagaggtt cgaaagtact gaacgccact aaaactaaac
    11821 gtctagtgcc taaatatgct gatggtatag gaaacataac gacggtttca tctacgctaa
    11881 acttagatgc tttaatttta gctattaacg agctaacgac ggtattgaga actcaactga
    11941 tggcaggaac gactgatgct aaagatacta aactatcaga tgaaacaaaa gttgccgatc
    12001 cgattattcc ggacagtctt tctgaaaaat cagatcagta tctaggaatt ggagctcaat
    12061 ggttaactaa tctaatgaat ggttggaact cagcggttcc tcaatatatg aatagtgaaa
    12121 tggtttttat tacaaactat ctcaatgcgc taaaacttca aaacaatcct aattatacgc
    12181 aaggagcaac atggaataag aacttactga atggatggaa tagtttaact gggaccttta
    12241 ttgctacaat caattcattt tgtaatcaag caatggtcac gcttagaaac tacaacactc
    12301 ctatgtacaa caatggccga acgtggcagc aaaataatct taatggttgg aattcattgt
    12361 atggatcatt catagcccgt gtaaatcagc ttggtaacga ttcgattaac aaccttcgtt
    12421 ctaaaagtgg tggtttttac aatgcaggga cattcttgtt gcaatcttta ataaacggaa
    12481 tgaattcgtt aggtaattct ctttctacta caatgaacaa tgtcgctaat acaatggttg
    12541 gtggtatagg caaaggagtt aatggcgtca tatcgggagt taactatgtg cttaaggaag
    12601 tcgaatcaag taagaatatc ggaaactggc caattccaca atacgctaag ggaactgatg
    12661 gacatcctgg tggacttgct atgattaatg atcaaaaagg cctagttcac gaagaatatg
    12721 ttcagatgcc agatggacgt agttttattg ccaaaggtag agatcttctt gttaatttgc
    12781 ctaaaggtgc tcaggtctta aatgcttctt taacaaagaa attaaaggaa cgtttaaata
    12841 ttccacacta cgaaaaaggt attggcaatt tagatattgt tgatttgtta gatgatgaaa
    12901 aaagaatgtt agaattctta actagcaaag ttgatttttc aggcattaat gaaccatggc
    12961 ttgatatgac taaatcagga acgtcattaa tgtccaaagc tgcaaataca atgcttcaat
    13021 caaaattaag tgaattcttc actcatggca attttgatgg tgcagtaaat gccaatggtg
    13081 tttatcaata tttagttgat gtggcacaga aagtaatggg taagttcccc ggacttacag
    13141 taacttctgg atatcgagca ggagatgcct actatcacgg aaaacggcaa gctatcgact
    13201 tggcttatcc aggtatttct ggagatccga gatatacagc agcagcaaat tacgcttttg
    13261 aaaaattccc ttcaaaaatt gcgtatgtca ttacgaatgg acgtgtacgt gaccgcatgg
    13321 gattatctgg aacaaggtca agcggacaat ggacgaattg gccagatggc gatcacttcg
    13381 atcatattca tttaaacggt tcgatggggt ctggcgatat attcaatgga ggagttggag
    13441 gaagtggtgt tgcacgctgg cgttcttatg tgtctaaagc attgaaaatg aatggattac
    13501 cagctacagc agcgtatata aatgcctgga tgtcccaaat ccaaacggaa tctggtggta
    13561 atgagaaagc aatcggcggc aatgatgggc tcgccgaagg caatgcaaca ggattacttc
    13621 aaacgaaacc cggaactttt gcagctaacg cttttcctgg tcacggaaat attatgaacg
    13681 gatttgataa catgttagca gcaatcaatt atgctaagaa acgttacgga gttgcaggta
    13741 tgttgcaagt tatcgggaaa ggacatggat atgccaatgg cggcttgatt accaaagacg
    13801 gactatatcg agcaggagaa ggaaataaac cagaaatggt tattccattg acaagaaaaa
    13861 caagagctat cgaacttatg ggtcaagcat tagccttcct ttctggaagc aataaaaaag
    13921 aaacgaaaag tgagattaat tctgtagata atagacatat cctacaagcg atagaaaaac
    13981 agtctcgtat gacaaatgat ctgctatcat tattactctc gttttttaaa agtaacaatc
    14041 gatcagataa agaactagct ttagatattc agaaaatttt aatcaggagg acatagtatt
    14101 tgaggacagt aatattaaga aataaattga atgaagaaat tgatttgtcc acagaagatt
    14161 attttgctac tgaaatgtcg aatctaggtt ttgaagttac aaaagagcat attggacaat
    14221 gggggagttt cagagaaata ggagagagta ttgaattgtc tgaatttcaa tcatcagtta
    14281 tgatttctgt tcacggattt agagaacaac aattatatgg ttcattagtt gaatttctgg
    14341 ctaatggacc ttttgaacta gaattttctt ttgattcaga aacaatgatc aggaaatgta
    14401 gtttgaaatc attatctaaa acagaaattg attcccaaag ctcattgcta acagatactt
    14461 tgaatctata ttttacttca agctggtatt ctattaagcg tgaaaaatta atacagaatt
    14521 caacttctct caatacacgt ggaaaagttt actcatttgt tcgaccatat gtctacacca
    14581 aaaatgtatg ggaaaagaaa ggtgtattta agttcagtaa taattctgta tatttgacaa
    14641 atagtacaga gcgaatgtct ccgttaaaaa taaggattat tggagagtgc tcaaatccat
    14701 attgggaagt tatccaaaac tctaaagtag tagcttcaga tggctatttt attaatatga
    14761 aagacacaca aatattagaa gtgtctagtt tgctagaaga ccaaacggca attttaaaag
    14821 atgtaggtgg agttgaatct tctgtttatc aacaacaaga ttttactaaa tcaaacttcg
    14881 tccaagcgcc tctaggagaa ttcagcgttg tttttcatgt tggacatgca aatgtagaga
    14941 ttgaacttta tgaggagcgt gatctatttt gattttagcc attaccctct ttcaccgaga
    15001 tatgaattta tataacgaac gtttaatttc gactgagttc gaatttggaa cggatgaaat
    15061 tggagaagaa gtaagtaatt ttattacaga taaatatgtc catgtaaaat caggagattt
    15121 tctactagca aaatttattc ctagcgggaa gattgcttat tttggagtga ttagctcgca
    15181 agaagacaaa aaaataaatt gcaaaggatt actcagtttg gccgatagcg agataccgac
    15241 tgtaaaaata tcaggtgcta attatgaaga gcatattcga cgactgattg aatactactt
    15301 attgaacgat ccaacaaaac aactaaaaaa tattttggag gtaaaaacag aaagcgttac
    15361 ttctcattct tatcaagctg ttgacacaaa taagagacga ttgaatgcat atataataaa
    15421 tgggttcaaa aaatataatg ttaaatggta tgtcaaagag attaaaggta aaaaaatata
    15481 tactggtatt cgagcagttg atgattcaat ttatattaaa gataattcat ctgagtttaa
    15541 tgactgggat gtttttgtgc aatctcctgg accagggaac gagaacaaat tattgatcgt
    15601 agataaagca atgaaagata tagaaaatcc cttggtattg tctacgtggt atttagatga
    15661 cgaaaataat cttacgcaag attcaacgaa taagaacatt gcattgccta ccgttaattt
    15721 agtaaatata tatgatcaaa ctcaagaaga tagaccttct tatgaagagg tagctaaatc
    15781 ggagcttaaa ggcaacgcct attcacatga aattaaagtt aatgttgtgc taaatgctag
    15841 aaatatcaat gtagaaaata tcgaaacagg aatgttagcg actattgttt acaaagagca
    15901 gacttacaaa tctgttctaa ctgcctggcg aatatccagc agtaaaaaga atatagaatt
    15961 gacatttggt aatgtccgaa gtcgttttat ggattatttt gaagataatg ggggataaaa
    16021 aatggttagc aatgtggatg gatatcaatt tgaaaacgtg aaagtaagcg cagaaaatga
    16081 tgctagactt tatcacgttt tatataatcg gaaaaatcag gttattgatg gttacgatca
    16141 gtctatgaat ttatcttcaa gcggattaac agtaaaagtt gctgcaggag cagcgattat
    16201 tcaaggtcgt atggtcgttg ttcgacaaga agaaagtata acagttccag caaactcaag
    16261 tggttatata gcattaacag tggatttgac acaagaagtt atacctggat ctattcttcc
    16321 agaatcggaa gaatatgaat ggactaataa tcaagtcaag ctagaattta taacaaaagt
    16381 tataaaaggt aatttaaaca atggtgataa ggtctataat ttaccactat gctcagttaa
    16441 ttctactgga tcaactgttt caatctcaaa gatatcggat agttacgagc tgactctctc
    16501 taaaggagaa attttgtgga gggggactgc gttaatgcat gatactcaaa ctgtccaacc
    16561 ttcaaaaaaa atttggcaga cagtttcagg ttttttgtta gtatggctcc catacgaaaa
    16621 cgggcaagca attgaggata gatatgtaac tacgcctttt tataaggagc gtataacttt
    16681 tactaatgtt ttaggagaaa ttgtttcagg atttgatgac tatcacaaaa agtggtttag
    16741 taaacgaata aactataatt cgaatactaa tatatttaca ggtgcagcaa gcaatgcaag
    16801 tggagataac gcaaatatgg tgcttaggta tatagtttct ttttagttag gaggtatgga
    16861 agagtggcaa atttagaaat taaattatct gcaaataaga gacagcctta tctacgtcac
    16921 cgtgttgttg gtagagttgg tgatgggggg ctcacaacaa tcaatgtaca acttcttgag
    16981 gaagatgaaa ttacaccttt tgtaattaat ctaaacggta ctttgaaatt tgtgggcgaa
    17041 gtttcaaacg gtaactatac cgagggagaa ccagaaataa tcgattcgac taatgggtta
    17101 attagttaca cgttcactaa gtcaaatttc agcacgagtc atcaattcaa acaagcatat
    17161 tttgaatatg tagatcctaa cggcaaaaaa gtaacttttc agaacttcat catagacgtg
    17221 ttagaacgag tagatattaa ttcggagcaa gcgaaatact atatttcttc attggaaaaa
    17281 ttacagagtg aaatgcaaac cactttcaat cagttcatta gtgataaaca ggtccaatac
    17341 gatcaaatct actcgaaata taacgaatta gtaagattga taaatgaatc agataagcaa
    17401 gtaaatgatc gtattgataa ggcaaataat cgtattgatc aaaccaatca gcaaatcggc
    17461 gatctcggca agctgaaaaa gatgtacagt aacagcattg a
//
LOCUS       DAJSAW010000055        17224 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJSAW010000055 DAJSAW010000000
VERSION     DAJSAW010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.055"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            1..369
                     /locus_tag="ONT64_002122"
     CDS             1..369
                     /locus_tag="ONT64_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR2303526.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="ONT64_002123"
     CDS             549..1490
                     /locus_tag="ONT64_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR2303527.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="ONT64_002124"
     CDS             1559..2290
                     /locus_tag="ONT64_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCR2303528.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="ONT64_002125"
     CDS             2434..4614
                     /locus_tag="ONT64_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR2303529.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="ONT64_002126"
     CDS             4634..5116
                     /locus_tag="ONT64_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCR2303530.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="ONT64_002127"
     CDS             5132..5893
                     /locus_tag="ONT64_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR2303531.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /locus_tag="ONT64_002128"
     CDS             6024..7358
                     /locus_tag="ONT64_002128"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR2303532.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7665..8150
                     /locus_tag="ONT64_002129"
     CDS             7665..8150
                     /locus_tag="ONT64_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HCR2303533.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8143..8826
                     /locus_tag="ONT64_002130"
     CDS             8143..8826
                     /locus_tag="ONT64_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR2303534.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8952..11597
                     /gene="polA"
                     /locus_tag="ONT64_002131"
     CDS             8952..11597
                     /gene="polA"
                     /locus_tag="ONT64_002131"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR2303535.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11642..12478
                     /gene="mutM"
                     /locus_tag="ONT64_002132"
     CDS             11642..12478
                     /gene="mutM"
                     /locus_tag="ONT64_002132"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HCR2303536.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12442..13071
                     /locus_tag="ONT64_002133"
     CDS             12442..13071
                     /locus_tag="ONT64_002133"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR2303537.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13123..13455
                     /locus_tag="ONT64_002134"
     CDS             13123..13455
                     /locus_tag="ONT64_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR2303538.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13767..14258
                     /gene="nrdR"
                     /locus_tag="ONT64_002135"
     CDS             13767..14258
                     /gene="nrdR"
                     /locus_tag="ONT64_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCR2303539.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14281..15654
                     /locus_tag="ONT64_002136"
     CDS             14281..15654
                     /locus_tag="ONT64_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCR2303540.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15654..16580
                     /gene="dnaI"
                     /locus_tag="ONT64_002137"
     CDS             15654..16580
                     /gene="dnaI"
                     /locus_tag="ONT64_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCR2303541.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16709..17149
                     /locus_tag="ONT64_002138"
     CDS             16709..17149
                     /locus_tag="ONT64_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR2303542.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5875 a         3008 c         3795 g         4546 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggtagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7561 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7621 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7681 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7741 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7801 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7861 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     7921 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     7981 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8041 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8101 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8161 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8221 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8281 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8341 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8401 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8461 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8521 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8581 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8641 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8701 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8761 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8821 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     8881 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     8941 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9001 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9061 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9121 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9181 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9241 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9301 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9361 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9421 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9481 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9541 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9601 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9661 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9721 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9781 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9841 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
     9901 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
     9961 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10021 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10081 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10141 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10201 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10261 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10321 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag
    10381 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10441 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10501 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10561 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10621 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10681 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10741 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10801 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10861 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    10921 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    10981 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11041 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11101 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11161 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11221 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11281 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11341 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11401 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11461 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11521 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11581 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11641 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11701 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11761 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11821 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    11881 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    11941 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12001 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12061 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12121 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12181 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12241 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12301 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12361 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12421 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12481 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12541 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12601 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12661 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12721 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12781 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12841 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    12901 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    12961 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13021 aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13081 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13141 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13201 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13261 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13321 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13381 gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13441 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13501 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13561 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13621 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13681 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13741 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13801 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13861 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    13921 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    13981 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14041 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14101 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14161 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14221 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14281 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14341 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14401 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14461 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14521 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14581 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14641 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14701 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14761 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14821 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    14881 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    14941 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15001 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15061 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15121 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15181 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15241 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15301 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15361 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15421 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15481 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15541 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15601 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15661 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15721 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15781 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15841 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    15901 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    15961 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16021 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16081 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16141 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16201 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16261 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16321 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16381 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16441 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16501 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16561 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16621 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16681 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16741 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16801 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16861 atttgtcgga acgattgaaa gtattactca agtagaagca gaagaagaaa cgcttcagtt
    16921 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    16981 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17041 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17101 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17161 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17221 tggg
//
LOCUS       DAJSAW010000056        17152 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJSAW010000056 DAJSAW010000000
VERSION     DAJSAW010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.056"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            138..1475
                     /locus_tag="ONT64_002139"
     CDS             138..1475
                     /locus_tag="ONT64_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HCR2303543.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="ONT64_002140"
     CDS             1523..3652
                     /locus_tag="ONT64_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HCR2303544.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="ONT64_002141"
     CDS             3627..4316
                     /locus_tag="ONT64_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303545.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="ONT64_002142"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="ONT64_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5713)
                     /locus_tag="ONT64_002143"
     CDS             complement(5546..5713)
                     /locus_tag="ONT64_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303546.1"
                     /translation="MYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKESEAFAKRIGG
                     RVKPIQEVRPD"
     gene            complement(5792..6232)
                     /locus_tag="ONT64_002144"
     CDS             complement(5792..6232)
                     /locus_tag="ONT64_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCR2303547.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6236..7081)
                     /locus_tag="ONT64_002145"
     CDS             complement(6236..7081)
                     /locus_tag="ONT64_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCR2303548.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7230..8648
                     /gene="pepV"
                     /locus_tag="ONT64_002146"
     CDS             7230..8648
                     /gene="pepV"
                     /locus_tag="ONT64_002146"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HCR2303549.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8705..9652)
                     /locus_tag="ONT64_002147"
     CDS             complement(8705..9652)
                     /locus_tag="ONT64_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR2303550.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9875..10225)
                     /locus_tag="ONT64_002148"
     CDS             complement(9875..10225)
                     /locus_tag="ONT64_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCR2303551.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10425..11504
                     /gene="pepA"
                     /locus_tag="ONT64_002149"
     CDS             10425..11504
                     /gene="pepA"
                     /locus_tag="ONT64_002149"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HCR2303552.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11648..11968
                     /locus_tag="ONT64_002150"
     CDS             11648..11968
                     /locus_tag="ONT64_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR2303553.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11990..12454
                     /locus_tag="ONT64_002151"
     CDS             11990..12454
                     /locus_tag="ONT64_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR2303554.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12659..13264
                     /locus_tag="ONT64_002152"
     CDS             12659..13264
                     /locus_tag="ONT64_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCR2303555.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13303..14592)
                     /locus_tag="ONT64_002153"
     CDS             complement(13303..14592)
                     /locus_tag="ONT64_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCR2303556.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15020..15499
                     /gene="rlmH"
                     /locus_tag="ONT64_002154"
     CDS             15020..15499
                     /gene="rlmH"
                     /locus_tag="ONT64_002154"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR2303557.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15702..16252
                     /locus_tag="ONT64_002155"
                     /pseudo
     CDS             15702..16252
                     /locus_tag="ONT64_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
     gene            16323..>17152
                     /locus_tag="ONT64_002156"
     CDS             16323..>17152
                     /locus_tag="ONT64_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HCR2303558.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT         5460 a         3105 c         3467 g         5120 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catatgggga tcacctgctt ttttcttttc attatacgaa ctactcgaca
     5761 caatacaaga aaaatgctta gacggtcttt attaagaatt tacatcatcc aaagaagcgt
     5821 acgcttggaa taactgatct aactccggat tgactttgct cagcttcact aatcgttgcg
     5881 aatcagaaag taaaaaacag tgtttactac tgccagtagt ttttaatttt ttgtcttcag
     5941 ttcccacaat ttcgtagcta aaatccaacc gtgtccccgt atacttttgg atagttggat
     6001 ggatgctcac tttttctccg taatggatca tttctttata gctgcaattt acttccagaa
     6061 cggggatgat gatcccagat tcttcgattt ttttataggg aaaccctaga tgtaccaata
     6121 aggctacccg agcctcttca aaccagcgga tataattgct gtgatgaata atccccattt
     6181 gatctgtttc ataataaaaa ggttctcgca cataacctgt aaatttctcc atgactcatg
     6241 cctcgttttc cattgatttt ttacaaaaat aattcatcca tttcggaagt gcttcgctga
     6301 tttgtgtcgg caaaacaata tattgttctt ttgccagctg gtctccaatg aaactatgaa
     6361 gataaatagc tgctgcaatc gtttggtcat tttgaggaaa ctgggctaaa aatcctgtga
     6421 tcatcccagc taaagtgtca cccatgccgc ccgttgccat agcagcagaa cctaaaggat
     6481 tggtgtagaa gtgctggtga ccataaattt ttgtctcatg acttttcaaa acgagcacag
     6541 caccgatttc ctgttgcttc tttttgtttt ctttatcccg ttgttcttgt atcggtagag
     6601 aactcaaccg ttgccactcc atttgatgag gtgtaagtac agtttgttcg ggatagggta
     6661 gcaaaagatg attagcagca agtaaactta aagcagagcc atcaatcacc agccattgtt
     6721 cttttgattg gttttctagt acttgtttga aacgttccaa actcttagaa gaagtaccca
     6781 atccgggacc aatcaagagt acatctgctt gctttattac ggaaagaaac tggtcagtat
     6841 ccgtccaaac tacaatcatt gcttctggca gatgactgtg aagagcactc cgattttttt
     6901 catcagttac tacagtcgtg agacccgctc cgctgtgtac ggcagctaaa gcactcataa
     6961 tgattgctcc gccatattgt tcattgccgc caatcaaaac gatacgtcca aatgttcctt
     7021 tatgtgaaat ttttggacga ggtgtgatga ctcttttcac tagttgttcg tttatcagca
     7081 tctaaaaacc tccttttact cattatagcg tatatttttt ttagagaaaa acagataaga
     7141 aaaggaattg tcagattttt tgaactattt ttttctgtaa tttgtggtat catgaacacg
     7201 aagacttaaa taagtggagg attaacttta tgacaattga ttggcaaaaa gaagtagaag
     7261 cacgtaaaga agacttgctt gaagacttga aaaatctttt acgtgtcaac agcgagcgcg
     7321 atgactcaaa agtaacacca gatgcaccat ttggacctgg acctcgcgac gcattgaaac
     7381 atatgttagc ttatggcgaa cgtgatggat ttaaagtaaa aaatgtcgat aactatgcag
     7441 ggcatattga tcttggcgaa ggagacgaaa cgctagggat atttggacac atggatgtcg
     7501 ttcctgcagg agatggctgg gacacagatc cttatgaacc agtaatcaag gatggaaaga
     7561 tctttgcacg cggatcaagc gatgacaaag gtccaagtat ggcagcttac tatgcgatga
     7621 agatcattaa agaacttgac ttgaaactat caaaaaaagt tcgcttcgta gtcggcagcg
     7681 atgaagaaag cggctggggc gatatggctt attatttcga acatgaagaa gaaccagatt
     7741 ttggtttctc accagatgct gaattcccaa tcatcaatgg tgaaaaagga aatgtttctt
     7801 tagctttacg cttcaaaggc gacaatgcag gagattatgt cttgaaatct tttgtttccg
     7861 gacttcgcga aaatatggtg ccaggaacag caactgcagc acttcaagta ccaagtgcag
     7921 atgcagctat cgcgatggaa gaagcatttt accaattcat cgaagcaaat ccagtaagcg
     7981 gaacgattga agcggacaat acgtatgtaa aaatcgaatt agttggtaaa ggcgcacatg
     8041 gtgcaagccc acaatctggt atcaatgcag gttctttctt ggcattgttc ttagataact
     8101 atgaattcct aggatctgct aaacaattta tccatgtagc agctgcttat gtccatgaag
     8161 atttttatgg agaaaaacta ggagtcgctt atgaagatga aaaaatgggc aaattgacaa
     8221 tgaatgctgg actatttgct tttgaagaaa atggtacaga ggaagcgaac ttcatcaata
     8281 tgaatttccg ttttccaaaa ggtgtaacag tagacggatt gcaaagtgac atcgaacaaa
     8341 cagtggggca agaaggcgca acagttactc gaggtgctcg cgtaatggaa ccacattacg
     8401 taccaatgga tgatccattg gtggctacat tattgcaagt ctatgaagac cacactggag
     8461 aaaaaggcta cgaacaaatc atcggtggcg gaacttacgg acgtttgttg aaacgtggcg
     8521 ttgcttatgg agcgatgttc ccaggatata ctgatacaat gcatcaagca aacgaattta
     8581 tgagtcttga tgacttgttc agagcaacag caatctatgc agatgccatc tatcgtttgg
     8641 cgaaataatt aattaaaaag aggatcaaga attgcaaatt atttttgcag tcttgatcct
     8701 ttttttactt tctactgtgg tattcatgat aaacatgata tagattcaat tcatattctt
     8761 tcttagcgtt cgtgacgact gtatccaaaa tcaaccctgt tactaatgaa agaagtgcta
     8821 aagtcatcaa tccagtactt aaaatcgcag aagggatgcg tgtaataaag cttgtcagca
     8881 tatattcgcg aatcacagga atccccgtga ccaatccaaa taaaaagaag aagatggtcc
     8941 aaatcccaaa gaaaagcaaa ggcttataat ctttaaacat tttaataatc atcatgatca
     9001 ctttgaatcc gtcagagaat gtattcaatt ttgattcgct gccttcagga cgatcacgat
     9061 aatcgattgg gatttcaacg aacttgaatt ttttatctaa agcatggatc gtcaattccg
     9121 tttcgatttg aaaaccagaa ctcataatag gaaaactttt gacaaagata cgattgaatc
     9181 ctcgatagcc ggtcatgaca tcaagaattt ttgttttata cattctagta attgtatttc
     9241 taacaaggtt atttccaaaa tcgtggaaag gtctcttatt ctcatcaaag taggttccat
     9301 ttgataggcg atcaccaatg accatatctg cttcaccaga acgtaatttc tcgagcaaac
     9361 catgtacagc ttctgcagga taagtatcat cgccatctac cattaaataa tagtcggcat
     9421 cgatatcaaa gaacatttgg cggatcacat ttccttttcc ttgacgaggt tcttttttta
     9481 cgattgctcc accttgaacg gccaattcgt atgttcgatc ggtcgaatta ttatcataga
     9541 cataaatatc tgcttctggt aattctcgtt taaagtctgc aatgactgtt gaaattgttg
     9601 cttcctcatt gtaacaagga atcagaacag caattttttc ttgttgttcc atttttcact
     9661 ctccttaact atagaaaaca aaagctaaat taaaaatacc tcataatcaa aggaaatgct
     9721 agtttttccc gctaaatatc atttatttaa aaagaaaata ctggaatata tgtttggaaa
     9781 gctctatcta aaaaaccacc cactacaaaa gaaggacatg atacctctct tttgaatagt
     9841 gggtgcgtat tcgttctctc acttttttga cgtattaatc tgttgcacct ttttttcttg
     9901 aaatttctaa tacggttcct gtatgagcat ctgctaaaaa ttcataataa acagtttcgc
     9961 catcttctat tcgagaaaga cctccccgat aagcactcga ccgaacggcg aatttcctaa
    10021 taggttcctt ttcaaaagag atccaagaac cttcgattgc accttctttt agaaaagatg
    10081 acttgatatt ttcaagtact tggtctgctg acaatacttt tttgcgatga taccaaatcg
    10141 ttgaagctac cccgctcaat aatcctagac cgattccaac tgctaacccg cctttaaaat
    10201 agcttagttc gctttcttca tacatgtgga catacctcct tcttctcact tgttactcct
    10261 tgtgcttatt ttaccacact ctcatatttt ctttgaaata aataaccgct accagaaaaa
    10321 ataagctata ataaatacga tcggtctgca gaaaaaatca tattttatcc gcagatcaat
    10381 ataagtctga gaggagttta gtctgaaatt tagacgaata gcatatggaa gaaaaaacat
    10441 ttcaacgaat caaagagtta acagaattac aaggaacaag cggttttgaa caagatgttc
    10501 gcgcttatat ggaaagtcat atggaaccgc tggtagatga gttacagtta gatggtttgg
    10561 gcggcatttt tggtctgcgc catcatgaag aagcggatgc accccgtgta atggtcgcag
    10621 cacatatgga tgaagtcgga ttcatgctga cacaaatcca agaaaacggg ctttttaaag
    10681 tcgtacctct aggcggctgg aatccttacg ttgtttctgc tcagcgtttt acacttaaaa
    10741 cctctactgg taagaattat ccatgtattt cttcttccgt tcccccacat ttattgcgag
    10801 gaacaagcgg acaaaaatca gtagaagtaa ccgatatttt gttcgatgct gggtttgaat
    10861 caagagaaga ggcaatgagt tacggtgtcc ttccaggaga cacaatcgtt ccttacgctg
    10921 aaacgatcaa aacagccaac ggaaagaata ttatcagtaa atcatgggac aaccgctatg
    10981 gctgtacaat ggtattagaa gctttagaag cgttgcaaaa tgaaacatta ggtcatacat
    11041 tgattgcagg cgcgaatgtc caggaagaag taggcttgcg tggttctaaa gcttctgtca
    11101 ataaattcaa gccagatctg ttctttgcag tagactgctc tgcagcagat gatacagtga
    11161 cgaaaaatgg cacattcgga catttaggag aaggtacatt gatgcgtatc caagatccag
    11221 gtttgatcat gcttcctcgt ttgcgcgagt acttgctgga tatcgctgaa acaaacaata
    11281 tcccttatca atactttgtt tcaaaaggcg gtacagatgc aggtgctgct catacacaaa
    11341 acgaaggaat cccaagtaca gtgattggcg tagtgggacg ttatatccat acgcatcaaa
    11401 cgatgttcag tattcgcgat tttgaagctg cacgagaaat gttgattcaa acactgaaag
    11461 gtttggataa atcaacagta aatacgatcg tatacggaaa ataatgcgat agtgaaacta
    11521 taaaatctgg caaaagattg ctttttgcca gatttttttt ataaaaagag gatcaatgtt
    11581 tgttagttcg aaagaaaggt gtataattta attgaaaaat acataaacag aaagtagagg
    11641 acaaagaatg attatcccaa atagtttaga agaattagca ggctacgtag aaactggaaa
    11701 gagtgtattt ttctttactg ccggctggtg cggagactgc cgttttatca aaccgcaaat
    11761 gcctgaaatt gaaaatgact tttctgattg gcaatttatt gaagtagacc gtgacaagta
    11821 catagatgta gccgctgaat ggaacatttt tggtatccca agttttgtcg tgatccaaga
    11881 cggaaaagaa ttaggtcgtc tagtgaataa agatcgtaaa acaaaacagg aaatcgaatc
    11941 gttcctagaa agtgtaacaa aaggctgaca tctgttagga ggggtcaaga tgaatcaaga
    12001 atataagaat attttagtag gtatcgacgg tagtgaacaa gcacttgctg ctttcaaaaa
    12061 agcggtagaa gtggctcgcc gtaataatgg gaaagtatat gtagcaaacg taatcgatca
    12121 gcaattttat aactttatgg gctatgcacc tattgatcaa aatctagtcg atcagcaaac
    12181 tgaaacagcc aaggaaatga tcgaagaatg taaagaatat gggaaatctg ttgattacac
    12241 aaatatagaa ggaattattg catatggttc tgttcgtgaa tccatggcag tcaaattgcc
    12301 aaaaaaatat gagatcgatc taatcatggt tgggcagtca ggtctaaatg cagtcgaacg
    12361 ctttatgaca ggaagtgtag ccagctacgt gatacgtcat tcaccttgtg atgtcttgat
    12421 cgtttctgac gaagaagaac agccaagtaa ttaagcaagt ggaacttgta cgaccttttt
    12481 aatcaattaa ctaaaacagt aaagggactg tgacaaaagt cttgtcacag tcccttgtta
    12541 cgaataaatg gtgttccaga agaggcttct tcggaaataa agcatgaaaa aaatttcatg
    12601 cagtgaatgc aattttttgt taagataaat ctcagcaaag gataatggag gaaagaacat
    12661 gatttttgct tataacaaag cacacgtcgg agacaccttg atggttatta tagccgacga
    12721 taaaggaaca gaaaacactg ttgaacgcaa atggaatgtt gctcggatca aagatgaaca
    12781 aggtcaagtt gttgcttgga actttttcca tatctccgat catctaacaa tcgaagggaa
    12841 cggccaagta agcttaacag atgaggaatt agcgaaattg aatcgattga tcaaagaagc
    12901 aggattcgaa gaacagcttg aagctgatca tgaaccgaag atcgttgtcg ggtacgtaaa
    12961 aacatgcgtc ccacatcctg attctgacca tttatcgatt actgagacag aagtagacaa
    13021 tggtacagta ttgcaaatcg tttgcggtgc acccaatatc gaagctggac aaaaagttgt
    13081 cgttgcaaaa ccaggagcta tgatgccaga tggaatgatg atctggccag gaactttgcg
    13141 aggggtagat agctatggaa tgatctgttc cgctcgtgaa ttgcatttgc caaatgctcc
    13201 agcaaaaaaa ggaatcttag tattgcctga ggatgccgta gtcggagaag catttccaaa
    13261 ataaagaggt tgtgacaagt ctttgtcaca acctcttttt tattaatttt cactattttg
    13321 attttgtttt aatgccgatt gatcgatcgt caattcaaca gtagttgttt tttctttcga
    13381 gccttcatag taagtgattt tgacagaatc tcccactttt ttcttataca aagcagattg
    13441 aagttctaca ccagaagaaa cttctttgtc atcgatctta gtaatgacgt cgtattgttt
    13501 caatccagct ttttcagccg gtgtagctgt ttggacattt gtcacgatga caccgttagt
    13561 tacagatgaa ggaatcttca agatttgttc ttgttgctga gtagaaacgg cagataagtc
    13621 gaccattgtg atacctagag ctggacgtgt cacttttccg tctttttcca gttgattgat
    13681 gatattcact acatcgttac ttggaatagc aaatcccatt ccttctacgc ttacattaga
    13741 agtagattca gaagtacttg cgatcttact tgagttgata ccgataactt gtccttcgat
    13801 attaactaaa ggaccaccag aatttccagg gttgattgct gcatctgttt ggatcgcatt
    13861 gatattgact gtttcgttcg attcattcgt acttgtcact tgtcggttca aggaagaaat
    13921 gattcctgaa gtgacagaat ttgcatattc agatcctagt ggggaaccaa tcgcaatcgc
    13981 aggctcacca accttcagag cacttgagtc accaaaagag gcaactgttt cgactttgtc
    14041 tgaattgatc ttcaacaccg ctaaatctga ataagcatct gttccaacta attcagcttt
    14101 taccttcgta ccgtctttca tcaagacttc aagaccttgc tggccatcta ctacgtgatt
    14161 attcgtgact acataagcag tattcccgtc ttttttgtag atcacgccgc ttccttcact
    14221 ataagcttcc aaattactat catctgagct gctactttct tcttgctgcc cgaatagtcc
    14281 gccaaaaccg cttgattggt tttgactttg taagttgata acagaaacga cagcgtcttg
    14341 aactttatct accgctttgg tgatatcaga atctacattg actttgacat tttccacaac
    14401 tgtttcaccg gcagaattct gattgccgct tgtagcagta gaactattgt tgccagtgcc
    14461 catcgctgca taaaaaatgc cagctgtcag tagtcctcca acgatccctc caacaagccc
    14521 cagacctaac ttacgccaga ggctattatt tttacttttt ttcatctttg gtgtcacatt
    14581 ttttctatcc atgtgttcct cctccaatta cattcattta tctactgtta gtatagtctt
    14641 atttgttgac tttagtctac tttgagactg tgaacttttt atgaaaaact ttaggaaaaa
    14701 tcattgtttt cagatgaaaa tatttgattt tctttttcca cagatactgt gtaaaactac
    14761 gcggatagga ttggaaaaat aagggataaa acatatccac agcctgtgta taaagtggat
    14821 aaattcttat aaaacgtatg tttgcctaat gaatgtgatg aaataaggta aaataacact
    14881 gttttctgtg gataaacctg tggatgttgt gcataactgt tggggaaatc tttttaacaa
    14941 aaatagccaa aataaacata tagacgaaaa aattgattat aataaacaaa gtgagcatga
    15001 agaccaaagg agagaaaaca tgaatatcaa aattatctca gtaggaaaat taaaagaaaa
    15061 atacctgatc caaggtatca atgagtatgt caaacggctg aatgcctatg caaaaatcga
    15121 attgatcgaa gttcctgacg aaaaagcccc ggagaattta agcgaagcgc aaatgagaca
    15181 agtaaaagaa aaagaagggg aacggatctt agcgaaaatc aaagaacaag aatacgtcta
    15241 tgccttagca attgaaggga aaaatcctac aagtgaatct tttgctaaac aaatcgacca
    15301 attaggtatc caaggaaaaa gccaccttgt ttttgttatc ggcggctctt taggactcag
    15361 cgaagccgtc atgaaaagaa gcaatgctca gatctctttt ggaaaaatga cgtatccaca
    15421 tcaattgatg cgtctgatcc ttgtcgagca aatctaccgt gccttccgga tcaatgcggg
    15481 ggctccatac cacaaataat gatatttttt aagagaaaaa attatcccta ttttaaataa
    15541 tagcaatagg tagtggtagg gagaaattta taaataactt ttttatttgc tttggtagcc
    15601 aagtaagttg caaaagaaaa atagatactg ttaaaaagat aatgtagaca tagaaacaaa
    15661 tacgtaaaat tttagttaga ataatggcaa ggagaaaaaa catgatagat gaaaaaaagt
    15721 gtttaaaaaa tctattagag gatatggatg atattgaaga aaactatcta aatggactgg
    15781 gaaaacaaat atatttcaga tacttaagct atctaaaaat gaaataagac atagtaattt
    15841 tttagctttt ttgtttgctc caaatgaaac ccacaattta tctgatgagt tttttaaaat
    15901 gtttttgaaa aggtatatag attctaatga tgataccaaa gctgctatca attattttga
    15961 tgctttactt aatagctatg aagatttaat tgtttacaga gaaaataaga atattgatat
    16021 tttattggta tcggaaaaaa ataaaatagt ggtgtgcatc gaaaataaaa ttctgtcttc
    16081 ggagtctgga gggcaactta ataaatacca aatatatata gaacgcaatt attctaacta
    16141 taaaaaaatt tttgtttttt taactccaga tggaaatgaa cccacaaatc ctagttgggg
    16201 cataattact tacaagaata tcgttgaaga gagtgtaaaa tattttgtgt aaatagaaaa
    16261 aaggaagtcc cttctgtaga atagagttac cacaacacat tcacagaaaa gaggacttcc
    16321 atatgaacga ttttactaca gaaattatgg aaatactaat caataaaggt gatttggatg
    16381 atttgtttcg tcgtcaccta gaattggcta ttaacgcttt attacaagct gaattaacgg
    16441 tatttcttga ctatgaaaag tatgatagag ctggatttaa ttcaggtaat tcccgtaatg
    16501 ggaattattc gcgctctttt aaaacagagt acggtgaatt gaatttagta atacctagag
    16561 ataggaatgg agacttttct caacaaactt tacctgctta taaaagaacc aatgactctt
    16621 tagaaaccac tattattcag ctagttcaaa aaggaattac tatgtccgaa atctctgatc
    16681 taatcgaaaa aatgtatggt cattattaca aaccacaaac tatttcaaat atgagtaaaa
    16741 ttgtgtctga agatgtttcg gcatttaaag aaagaacttt agaagctaaa tactcagtca
    16801 tttttatgga tgctactcat attcccttaa aaagacaaac cgtatcaaaa gaagctgtct
    16861 atatagtaat tggtatcagg ttggatggaa ctaaagaggt tctaggattt actatagctc
    16921 caactgaatc tgcttatgtt tggaaagaga ttcttcaaga tctaaaagac cgtggtttag
    16981 aagaggtttt gttagtcgta acagatggtt taagtggtat tgacgatagt attcatagtg
    17041 tttatccaaa tgctcaattt caacaatgtt gtgtccatat atctagaaat atcgctcata
    17101 aggttcgtgt tagtgatcga caagaaatct gtagcgattt caaattagtt ta
//
LOCUS       DAJSAW010000057        16786 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJSAW010000057 DAJSAW010000000
VERSION     DAJSAW010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.057"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..164
                     /locus_tag="ONT64_002157"
     CDS             <1..164
                     /locus_tag="ONT64_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2303559.1"
                     /translation="KDGLPKEMDFNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLS
                     YVNGKFCLA"
     gene            complement(262..1998)
                     /locus_tag="ONT64_002158"
     CDS             complement(262..1998)
                     /locus_tag="ONT64_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCR2303560.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(1995..2699)
                     /locus_tag="ONT64_002159"
     CDS             complement(1995..2699)
                     /locus_tag="ONT64_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2303561.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            2830..3333
                     /locus_tag="ONT64_002160"
     CDS             2830..3333
                     /locus_tag="ONT64_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HCR2303562.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            3293..3631
                     /locus_tag="ONT64_002161"
     CDS             3293..3631
                     /locus_tag="ONT64_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCR2303563.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            3652..5070
                     /gene="ffh"
                     /locus_tag="ONT64_002162"
     CDS             3652..5070
                     /gene="ffh"
                     /locus_tag="ONT64_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR2303564.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            5182..5760
                     /locus_tag="ONT64_002163"
     CDS             5182..5760
                     /locus_tag="ONT64_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR2303565.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            5764..6285
                     /locus_tag="ONT64_002164"
     CDS             5764..6285
                     /locus_tag="ONT64_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR2303566.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(6364..6918)
                     /locus_tag="ONT64_002165"
     CDS             complement(6364..6918)
                     /locus_tag="ONT64_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR2303567.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            7171..7446
                     /gene="rpsP"
                     /locus_tag="ONT64_002166"
     CDS             7171..7446
                     /gene="rpsP"
                     /locus_tag="ONT64_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR2303568.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            7458..7709
                     /locus_tag="ONT64_002167"
     CDS             7458..7709
                     /locus_tag="ONT64_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HCR2303569.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            7834..8625
                     /locus_tag="ONT64_002168"
     CDS             7834..8625
                     /locus_tag="ONT64_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303570.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            8795..9316
                     /gene="rimM"
                     /locus_tag="ONT64_002169"
     CDS             8795..9316
                     /gene="rimM"
                     /locus_tag="ONT64_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR2303571.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            9306..10067
                     /gene="trmD"
                     /locus_tag="ONT64_002170"
     CDS             9306..10067
                     /gene="trmD"
                     /locus_tag="ONT64_002170"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR2303572.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            10203..10550
                     /gene="rplS"
                     /locus_tag="ONT64_002171"
     CDS             10203..10550
                     /gene="rplS"
                     /locus_tag="ONT64_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR2303573.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(10682..11818)
                     /locus_tag="ONT64_002172"
     CDS             complement(10682..11818)
                     /locus_tag="ONT64_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2303574.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(11934..12848)
                     /locus_tag="ONT64_002173"
     CDS             complement(11934..12848)
                     /locus_tag="ONT64_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCR2303575.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(12939..13367)
                     /locus_tag="ONT64_002174"
     CDS             complement(12939..13367)
                     /locus_tag="ONT64_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCR2303576.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(13372..13746)
                     /locus_tag="ONT64_002175"
     CDS             complement(13372..13746)
                     /locus_tag="ONT64_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2303577.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            14032..14217
                     /locus_tag="ONT64_002176"
     CDS             14032..14217
                     /locus_tag="ONT64_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2303578.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            14229..14477
                     /locus_tag="ONT64_002177"
     CDS             14229..14477
                     /locus_tag="ONT64_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303579.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            14508..14687
                     /locus_tag="ONT64_002178"
     CDS             14508..14687
                     /locus_tag="ONT64_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303580.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(14730..15200)
                     /locus_tag="ONT64_002179"
     CDS             complement(14730..15200)
                     /locus_tag="ONT64_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303581.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            15287..15985
                     /locus_tag="ONT64_002180"
     CDS             15287..15985
                     /locus_tag="ONT64_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HCR2303582.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            16163..16504
                     /locus_tag="ONT64_002181"
     CDS             16163..16504
                     /locus_tag="ONT64_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303583.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            16497..>16786
                     /locus_tag="ONT64_002182"
     CDS             16497..>16786
                     /locus_tag="ONT64_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCR2303584.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         5535 a         2934 c         3274 g         5043 t
ORIGIN      
        1 gaaaagatgg actaccaaaa gaaatggact tcaaccaagt caatcaagga ttcatctcat
       61 ccgttgcgtc caaaagaaac catatcccta gaaaatcact aaattaccaa acaccattag
      121 aagttttttt gagttacgta aatggaaagt tttgtctcgc ttaatttgac aaataataga
      181 gtaaaaaaga acaaccactc gtgtatcttt cgaagcagga tgagtcatcc aactttaaaa
      241 cgcacgagca gttgctcttt tttacatttt cggaatggta acggtgaaag tcgaacctgt
      301 tccaagatga ctgtctacag taatcgtacc attaaggagt tgcacgtagt cttttacaat
      361 cgctaaccct aatccggtcc cgcctgaatg tcggcttcgc gccttgtcta cgcggtaaaa
      421 acgctcaaag atccgttcct gatcctcaat gtcgatacct ataccaaagt cttggacaga
      481 aaacgataga tcatctgttg cctgatagcg gataataatc tcgctgtcgc tttttgaata
      541 ttggacagca ttttcaatta gatttttgat aattggataa aggatatctg ttttagtagt
      601 aaatggttcg ttctttccaa tcaatcgaat cgtcaaccgt ttcttttcga tgattggctg
      661 ataattttga acgatttgct ggaaataagg ttctgtataa attgtctgga tctcgtaaga
      721 attctcgcca tcttttgaca gctggatgat ttcccgaatc agtttatcta accggatcgc
      781 atctttttgc atgatctcaa gaaaagaagt cagagtttgc gggtcatctt ttgcaccatc
      841 cagtaaagtc tcggtaaaac caatcagtga agtcacagga gtttttaatt cgtgagagac
      901 gttgccaacg aaatcctttt gtagcttttc taaacgtcgc actttcgtta agtcatacgc
      961 cacccctaga atctgtccgg taccttctga attagtaaag tagcgcaaac tcaagtctaa
     1021 tgtctgccga ccttctgtaa gggtgatctc ctgatggatc agcggatgct caggagtcac
     1081 ttgatgaatc agctgaatga gttttggctc ttgaatgact tctgtgtacc gctgttcagg
     1141 ttgatagctt accacgttta aatgtagctg catctttgga ttaagcagta gtaaacgaga
     1201 atcagcatca tcaatcagga atacgccaat catcagttcg ttcagtaacg tatataattg
     1261 ctcttctgtg gtagtacacg cccgatacat cttactcatt tgttcgctca acgcattaat
     1321 cgtctgatac aattcttccc attgatcggg tgtttgcatg attacttccg cctgattagg
     1381 attttgcacc atttttttta gtacgggcag cacggttttc aatggctggt ttttttgata
     1441 gatcagataa tagatcattc ctgcaagaac aaggaagaaa gacagaaaga agaaaaagac
     1501 ccaacggcga aaactctcag ttctcggtaa aaaaccgctt gtcggttctg cgatacgtaa
     1561 aatggcttct aactggccac tttttttgac tggtaatgcg acatataaaa gatcctcatt
     1621 caaagtagta cttttgcgca aagcgctacc taggttccca ccattcaata cggctttgat
     1681 ttcgggacgg gagtttcgct gttcattcaa tgtttcattg tttgtgtcaa ataagatctt
     1741 tccagtagca tccattaaag taatccgttc gttcgactga tggacgaatt cttccaaagc
     1801 agttttgttt tgcgctgctt ggaggttttc gacatctaat tgccggatca gcagttgtgc
     1861 tttttttgtc aggtagcttt cttgctgttc cacgatctgc tgttgaaaat aatgggaaat
     1921 caattgccag ccaccaaaaa acaaactaac taaaagaata agaaagaaga gacgttcata
     1981 aatttttttc catttcatcg tttgggttcc tgaaacttat agccaaatcc tctaatagtc
     2041 accaagtact ttgggtgttt tggatctcgc tcgatttttt ctcgcaaatg actaacgtgt
     2101 acatctacga ttcggctttg tcctgcgaag tcaaaattcc aaatgcggtc tagcaatgtg
     2161 tctcggtcga tcacacggtc ttttcgtttc atgaaataaa caagtaattc aaattctttc
     2221 ggtgttagtt caatcagatg gtcgtctacg gtcacttgat aattggtcaa atcagcagtg
     2281 atttgtccaa tcgatagata ttcaggctca gcttcatctt ttgattgctt acgaggttca
     2341 attcgtctga atattgcttt catacgggct aatacttcgc gaggactaaa tggtttggtc
     2401 aaatagtcgt cagcacctat ttctaggcca atgatccgat ccacttggtc atctttagcc
     2461 gtcagcatca aaatcggtgt ttcatttttt tcttgacgca gccgctgagt gatctccatg
     2521 ccatccatcg ttggaagcat cacatctaaa atgatgaaat caaatgattg ttctaatgct
     2581 aattctaatc cttcgcctcc atctgtggca ctggttacct tgtagccttc tttttccaaa
     2641 ttgaacgtca agagcgttac aatagaaggt tcatcatcaa caactagtat ttttttcatt
     2701 caaatctcct ttaaataggt ttcattctgt ttatatttta gcatagaatc agtacaaagg
     2761 gctattttac aaaagaaata aatggtataa taggaaggca ctttgctgaa tagaaaggaa
     2821 gagcagtcaa tgattggaat cagtgcatgt ttaggcggtc tggcctgtcg ctatgatgga
     2881 cagaaaaaag aagtaccaga attgatggaa ctagttgaaa ctggagaagc tgtcatggtc
     2941 tgtccagaag tgatcggcgg attaccaatt cctagagatc ccgctgaaat cattggtgga
     3001 aatggattcg atgtgtggaa agatcaagca aaagtatgga cgacaagcgg cgaagatgta
     3061 acggaagcgt acaaaaatgg cgcgatcaaa gcctatcaaa aattgcagga aaaacacatt
     3121 gatctagtca tcatgaagga aaaaagccct tcatgcggaa cccattcgat ttatgacgga
     3181 agtttttccg gaataaaaaa aaatggtgcc ggagtggcta ctgcttattt tattcagcaa
     3241 aaaatgactg tgctctctga tgaagaatgg ctgaaaacta gaggtgagca gaatggaaat
     3301 cgaaaagacc aatcgaatga acgcgctctt tgaattttat tcgaccttat tgacagaaaa
     3361 acaaatgaac tacatggaat tatattatgc agatgatttt tcattaggtg agatcgcgga
     3421 agaatacgaa gtaagccgac aagcagtata tgacaatatc aagcggacaa gcaagatctt
     3481 ggaggattat gaaaaaaaac tccatctttt ttctaattat attgttcgag aacaggtatt
     3541 ggaaaatatg actgcatata ttacagataa gtacccggaa gataaaaaac tacttgaata
     3601 tgtacagcaa attcaagcaa tcgaagaata aataaaagga atgaagaaaa tatggcattt
     3661 gaaagcttaa ctgaacgtct ccagcaggcg atgggaaaaa taagaaaaaa aggaaaagta
     3721 tcagaagctg acgtaaaaga aatgatgcgg gaaatccgcc ttgccttact agaagccgat
     3781 gttaacttgc aagtagtcaa agattttact aagcgcgtac gcgaacgtgc ggtaggggca
     3841 gaagtgctgg aaagtttatc acctgcacaa caaatcgtaa aaatcgttga tgaagaattg
     3901 acgatcacac ttggttctga aacagcagag ttaaataaat caccaaaaat accaactgtt
     3961 atcatgatgg ctggtcttca aggggcaggt aaaacgacct ttactggtaa attagccaat
     4021 tatttaaaga aaaatgaaaa tgctcgtccg ctcctaatcg caggggacgt atatcgacca
     4081 gctgcgatcg accaattgaa agtattaggt caacagctag acgttcctgt atttgatatg
     4141 ggaactgaag tcagcccggt agagatcgtc cgtcaaggga tggagttagc aaaagaaaag
     4201 aaaaacgact atgtattgat cgatacggca ggacgtctgc atatcgatga aacattgatg
     4261 gatgagttga aacaaatcaa agaattgacg caaccaaatg agattttgct tgttgtcgat
     4321 gccatgactg gtcaagatgc cgtcaacgta gctgatagct tcaaccagca attaggtatt
     4381 acaggggtcg tcatcactaa acttgatggg gatactcgag gcggggcagc actatcgatc
     4441 cgctcagtga caggcgcacc aatcaaattc atcggttctg gtgaaaaatt gactgactta
     4501 gaagtattcc acccagatcg tatggctagc cgtatccttg gtatggggga tatgctgaca
     4561 ttgattgaaa aagcgcagca agactacgac gagaaaaaag cggaagaact tgcacagaaa
     4621 atgcgggaaa attcattcga tttcaatgac tttattgaac agcttgatca agtgatgggt
     4681 atgggcccac tggaagatct aatcaaaatg atcccaggaa tgaatcaagt ccctggaatc
     4741 gaaaacgtca aggtagatcc aaaagatgtt gaacgcaaaa aagcgatggt ttattctatg
     4801 acgccagcag aaagagcaaa tcctgatctg ttgaacccaa gccgtcgtcg ccgaattgct
     4861 gccggttctg gaaacagtgt tgttgaagtg aatcgaatga tcaaacaatt caaggaatca
     4921 agaaaaatga tgcagcaaat gtccaaaggg gatatgaaca tcccaggtat ggaccaaatg
     4981 tttggttcag gcgtcaaagg aaaactaggc aaaatggcga tgaatcgaat gatcaagaaa
     5041 aataagaaga aaaagaaaaa acggaaataa gatgaaaaaa acagcttcgg tacagactga
     5101 aaatgtctac tgccggagct tttctatcag caatttttat tctttactaa tacaattact
     5161 ctaaacgaag aggtggcaca gatgagatgt ccgtggagtg aacaaacaga aagtatgcgg
     5221 caatatcatg atactgtctg gggtgttcct gaatatgatg accaaaagct gttccgaaaa
     5281 ttgatgcttg atattaatca agccggattg agctggcaga cgattttaaa taaaagtgaa
     5341 gcattcgatg aagcatatga tcgattcgat atcaaaaaag tcgcaaatta tggagaaaca
     5401 aaaatcagtg aactaatgga aaacaaagga attattcgta accgaagaaa aatcgaagca
     5461 gccgtccaaa acgccaaagc agtcttgaaa attcaagaag aaatcggcag tttttcagag
     5521 tatctttggt cgttttctaa tggaaaaatc gttgattctt cttatcattc acaagaagaa
     5581 gtcccaacaa caagtcctct ttcagatgaa ttagcaaaag atctaaagaa aagaggattt
     5641 aaatttatcg gaagcacaac aatctatgct tttttagaag ctgtcgggat catcaatgat
     5701 caccttgaca gttgtttccg caaagaagag gtcgtacgta tcggcagaaa gggcgaataa
     5761 agcatgcgaa aaatcatatt ttatggtgct ataagtttag atggctattt ggcaacgaaa
     5821 acggatgatc ttcaatggct ttttgacact ccaacaggta agcaaactac ttatgaagct
     5881 ttttatgaaa caatcgatac gaccatcatg ggcagaaaaa cttatcagga agcaaaaaaa
     5941 tatttggaga ccgaagcaat ttatccagag aaaaagaatt tcgttttttc cacccacgca
     6001 caattacaat tgccagatgc tgaggtcgta aacgaagatc ctgttgcttt tctaaaaaat
     6061 ctacaaatga cagaaggaaa agatatctgg gttgtgggtg gcggtcgatt gatcaaaccg
     6121 ctattggaac atcaaatgat tgatgaatgg tatatccaga ttacccctgt attattaggg
     6181 gacgggattc ctttattcca aaaaggaaca tatgaagcgc gttttgaaat gctagacacg
     6241 acacgattcg gtgaatttat cgaattgcat tatgtacgaa agtagaagta aataaaataa
     6301 aaaaggcaac tatatgcaga aatgtcctgt acatagttgc cttttttatt accgttatgc
     6361 tttttactct gttaatttgt tagaaaaatc tagatacgca tctaaaatcg tttggaagaa
     6421 acttaatgtt ccttcaacga tattaccatt ttcatcaatt agattcgcaa cgtttccgat
     6481 atatgcttcc ggttgctgca aggttggcac attcaaaaag acaagtgatt ggcgtagatg
     6541 gtggtttgcg ccaaagccgc cgatcccacc tggtgatgca gtaacgacta aacctggttt
     6601 atgatcccaa acactgtgtc cataaggtct tgagcctaca tctaaagcat ttttcaatac
     6661 tgctggcaca gaacggttgt attcaggagt aacgaaaacg acaccgtcta gtcccctcac
     6721 ttcctcacga aaacgagtcc attcagcagg agcctgttct ggagtttcga gatcctcatt
     6781 gtaaaatggc agatcatcga tttttacaaa tacactttcg tacccttcag gaagcaattt
     6841 cgcaaaagct tctgctactt ttttgttgta agaatctttt cttaaactac caataaaaaa
     6901 tccaattttt tttgtcatac gatttttcct ccttcaaact atctataata atcttacaaa
     6961 agataagtaa ctggagcaaa taaaaacact tactgacaaa agctacatag aagatagatt
     7021 acttattttt aacaaaaaag aagtctaaaa tcttctattt tctaaaaaaa atcgaattta
     7081 atcaagctgt aaagaaaaaa agctttacag ggtaaaaaaa aactgttaca ctacatcttg
     7141 tgaataaaac aatggaggtg gaaataaata atggcagtta aaatccgttt aaaacgtatg
     7201 ggttctaaaa agagtccgtt ttaccgtatc gtcgtagctg attcacgttc tcctcgtgat
     7261 ggacgtttca tcgaaactgt aggtacttac aatcctttga aagaccctgc agaagtagtt
     7321 ttaaaagaag acttagttct tgactggtta tcaaaaggtg cacaaccttc tgacactgta
     7381 cgtaacatcc tttcaaaaga aggcgttatg caaaaacacc acgaagctaa attctcaaag
     7441 aaataaggtg accggatatg aaagatttaa gcgacttagt cttaacaatc gttcgtccgc
     7501 tagtcactta tcctgatcaa gtcaagttag acgtcgtaga gtcgaaagac ttttacgagt
     7561 acaatctaac agtggctcct gaagatattg gccgtatcat tggaaaacaa gggcgtgttg
     7621 ctaaagcaat ccgcacaatc gtttacggtg ttcggatcaa tgcaccgaaa aaagtgcgtt
     7681 tgaatatcat cgatgataag gaaaagtaaa taaacgtgct gtactcgaat tttcgagtgc
     7741 agcttttttg ttttttttat taattgaaga aatgattatt tggcaaaaca agcaagaaag
     7801 cgtatcctta taggaggagg gagtgaacag ccaatgagaa agaaaatgtt atttattcca
     7861 tttgctctat tgttaagcgc ttgtagttcg gttactcaag aatcagacaa tacacatcta
     7921 tcaagcagta tatctgaaac tgttaccagt ccagaaaaaa gtagtaccaa aacggcttcc
     7981 accactgaac aaacaatggc tacctcaaat aatgagaaaa agactgcttt agaccagttg
     8041 aaagaacaac agccaaatgt tcccatgcct ctagatgtac cagtctcttc aggctatctc
     8101 aatatcgcag cgacacatac aaagcaaggt tattctattt tatattacag aacagatcgt
     8161 cccctcgggt tgaatgctga cgaattgaat caagaaacgc ctattgcaac ctatctatat
     8221 caatatgggt ttgcttctag tcaagaaaca atccaagttt tgcagccatt tgagatcgac
     8281 acaaatggtc agcaagtgga cttaggttct cgaatcacag ggtatcagca aggtgcagca
     8341 ggttcaagtt ttcttgaatg gcaagaagga aactggcgta ttcgaatccg tgggaataat
     8401 atagaaggcc aagatccctt actattagct aaagaaatcg tagcttattt ggaagaaaac
     8461 agtctaccag cacctgaaca atttggtaaa attactgttg atatgggtga tacgacgaat
     8521 cgagccgtag aagtaagctg gcaagaaccg aaaaatgcct atacgatcac tcatcaagat
     8581 cctatgtctg cgttgaagat ggcagtttct atgaagaggt tgtgaaatca gcgcgctgat
     8641 ctgagcccat catttaactc caagtcacag gaagtaatca aaaatagcac gccgcatttt
     8701 tgcataattt tggcttaata ccttaaaaga aaactagaat tgcctcttca cacatgctaa
     8761 aataaaacag acaaaaaaga aatggggata taagttgact gaatacttga atgtaggaaa
     8821 aatcgtaaac acacaaggta tcaaaggcga agtccgtgtt atctcaacaa cggatttccc
     8881 agaagaacgt tacaaaaaag gaacggtgct aacactattc caagacggaa aagcacctgt
     8941 ggaattaaca gtcaaaagtc atcgcaaaca taaaaatttt gacttattga gttttgaagg
     9001 acatccttct atcaatgatg tggaaaaata tcgggatggc atattaaaag tgtcaaaaga
     9061 caactctgta gatctagctg aaaatgaatt ctattaccat gagatcatcg gtctgcatgt
     9121 attagaagat ggaactgagt taggtaaagt aaaagaaatc ttgtcaccag gtgccaatga
     9181 tgtttgggtc gttcaacgtc cgaaaaaagc tgatatattg cttccatata tcgcttctgt
     9241 cgtcaaagaa gtcgatttag aagctggagt ggtccgtgta gaaattccag aaggactgat
     9301 tgacgatgaa gattgatgta ttgactttat ttccaagaat gtttgaaggt cctatggggg
     9361 aatccatcat cggtaaggct gtagataaag gattattaga tatcaatatc tctaacttcc
     9421 gcgattattc agataacaaa catcagacag tagatgatta tccatacggc ggtggagcag
     9481 gtatgctgct caaagtccag cccatctatg ataacatcca agccattgaa aaagctgcac
     9541 cggatgtcaa aaaaagagta attcttcttg acccagctgg taaacgattc gatcagaaaa
     9601 tggcagaaga attttcacaa gaagaacact tagtattcat ttgtggtcat tatgagggat
     9661 atgatgaacg tatccgcaca ttagtgacag atgaagtttc cttaggagat tatgtcctta
     9721 ctgggggaga attaggtgcg atggtcatga tcgatgcaac agttcggctt ttgccagatg
     9781 tgttagggaa tcagacttca gctcaaacgg attcttattc aaccggttta ttggaacatc
     9841 cccaatatac tcgcccggca gaatataaag ggatgaaagt accagaagta ttgacaaatg
     9901 gaaatcataa attgattgaa gaatggcaat taaaagaatc actaagaagg acctatcaac
     9961 gtcggcctga tcttttggaa acacttgaat ggacacctca aatgacgaaa tttttagaag
    10021 aaatcaagaa agaagaacaa gaaaaaacag cagatgaagc caagtgactt tttctaaaat
    10081 cttctttaca gtaggttgat aatatgttag tattttatgg tgagtgcaaa agcacttaac
    10141 tattacgata ttccgctgtg agaaaagctc ataagaatat ttggaataag gagaagaaaa
    10201 ccatgaatcc attaatcgaa gaattaacaa aagaacaatt acgttctgac atcccagcgt
    10261 tccgccctgg tgacactgtg cgtgttcacg caaaagttgt tgaaggtact cgtgaacgta
    10321 tccagttatt tgaaggtgtt gttatcaaac gccgcggtgc tggaatcagc gaaacttata
    10381 cagtacgtaa agtttctaac ggagtgggcg ttgaacgtac attcccattg catacaccac
    10441 gtgttgcaaa aatcgaagta gttcgctacg gtaaagtacg tcgtgcaaaa ttgtactacc
    10501 tacgtgcatt acacggaaaa gctgctcgta tcaaagaaat ccgtcgttaa tcatcgttat
    10561 cagactgaga aagaaccctt ggtattcaag ggttcttttt ttgttctctg tttttttata
    10621 ttaacgattt ctatttggga catttttgat taataaaaag gggcaaaaaa ggggcaacaa
    10681 gttacagttt ttctatatct tctttcaatt tttcttttac tccctttgtc acatgcaaat
    10741 aaatcttttc agtgatatca ctattttcgt gcccaacgcg atcctgaata gcatacagag
    10801 gagttcctag ttccgctaat tttgaaatat gggtatgtct aaaaatatgt gaactaagtt
    10861 ttttatcaat cttcatgtca gctttatgat ttctcaaata ggtgttaatt gctgttagtt
    10921 ggaaaggagt tcctttcgtt gtttgaaata gaaattgacc attcggattt aattctagaa
    10981 gctcattgta aatagctatc gctttttttg gtaaatcaat ttctcgcatt ccagcagcag
    11041 tttttgtaga atcagatttt ttcatatcag ctattgaacg ttctctatac atcatcgtcc
    11101 catttataac tactgatgca ttattattgg taatgtgtac atcgtcttta cttaatgcga
    11161 ttgcttctcc aggcctcatt ccagtcaaat atagccactg gaaaaggaga gaatatcttt
    11221 tattgtgtga agttgtgaaa tttactagtc ttttatattc atcatcttct aaaaacttat
    11281 ctttaatttt tatcgttttt gactctcgtt tataatcaat aactacttcg ttgataggat
    11341 ttttttctac atagcctttt ttcatagcgt atgaaaaaag aagattcaat ttggatttaa
    11401 ttacactgac gtacttattt gacaagtcat ctttatatat catatcttca aaaatattat
    11461 ttaaatctac tgtattaata ccagaaacga tgtaagtctc aggtatcttt ttctttatag
    11521 tgtttaagat attattcgta ggataatatg tagactcctt aacttgtctt ttgtaaataa
    11581 ctaaccattc ttcgacaagc tcatgaaatg ttagatcagg cttctgaagt gttttttcgt
    11641 ttaattttat atcaattttt ttatttaact ctagcaatgc cactttctga gtttctcgag
    11701 atttattttt ataagtaatg ctcacttttt ttctttttct agtttttgga tcaatatatc
    11761 gctcattata tttatatact ttttttccgt ttttatcagt tttggtttca atccacattg
    11821 ttttcatctc ctatttgttg ctataatagg aatggataag taagccaatt atagcaggtt
    11881 tatttttcat cacgtccaca aactttggcg agggaggggg cgtgtttttt tgtttagtag
    11941 ctacctttcc atgaagtatc tgcgaagaac acttcaaatg ttgttccatc atttttcact
    12001 tcaaaatatg ctgttccttg tgcggattta cccggttgga tggtttccga aaagtaatct
    12061 ctactagcta aatcagattt aatatctgat gaatcataaa attctaaata acttgaactt
    12121 gcatcaaaag gtgtagtccc tgtgttttct acaagaaaat ctacctttgc gtaaaatagg
    12181 ccttctggtt tatagaaatc atcaccgtaa tcttttgata cagactggac agttacgtta
    12241 atagagttac cattttcaga agtaaaagga actgcttctc ctatactatg tgcaactgat
    12301 gccgtctggg gttctgaaga cgaagttgta gttgtagttg tagtagagct tgaatctttg
    12361 gattttccat ctttatctaa gaaagttcgt tgattataat atacaacctg ttcagttttt
    12421 ggactaaccc aaattaacat tgtttcatca tcagaatcag ttgaataagt atattggagc
    12481 atctctaatt tttttatatc ttttgcacta tttccatgtt caattaattc agagtgctct
    12541 ttataataat ctccttgtgc atcctttgaa atagatacaa ttaattcatc ccgtgtgata
    12601 gcattgtcta attctttgta aacaaaatca tttgtccatt tacgtaacgg tgaaccaagt
    12661 gtctgataaa cttgagttaa atcatcacca acttttattt tatgaacgtc tgatgaagtt
    12721 agtttttttg tttcatattc agatgttgat gaaacagtat tttctttagt gctagcagaa
    12781 ttaggactag tggagcatcc gcttaatacc aagcctaaaa aaagcaaccc aaaaacaatt
    12841 tttttcattt ttaattcctc acttctatga tatgattttt ttgtaggatc ttagaaataa
    12901 ggtttttagt ccgtgttgca gcacggactt tttttacatt atataagaaa gcgataagcg
    12961 ctttctggaa gtccgtaaag attctttaat tcctcaattc gtttaggata ttgatcattg
    13021 tcttctttgt atagagaaac aatgagatta gcagcaaagc aattagcttc gctttcagat
    13081 ttgcttctag atgttcttgt tgatacatag taactggata agccacgatg aaaaatagcg
    13141 tgacctaatt cgtgagcgca aatgtagaat ctttcctcag agtctctcag ttcatcattt
    13201 aagaagatta ttgcacgacc tctaatttct tgaaactgtc ctttgggatt ctcgataaaa
    13261 ggaacgtatt gaattttaat gcccatcttt tcacaaatat aaaaaggatt agcggactgg
    13321 tatttccgct tcaactcctc gactaaatta atcgtatcca tctccataag ctcacatctt
    13381 tttgcctttt tctttgtctt ctttcacaat atcccagaag gttccaataa ggatatcttt
    13441 tacgcgttgg acctgttcag gcgtcagtgt ttctccgcca taagacatat tgacatttga
    13501 atctagtaat ttatcaagtt caactacttc atcttttgtt gcccagtcag gaacttggtt
    13561 tctaccgagt aaataatcag tagttacatt aaaataatct gctacaagtt gtagtctttt
    13621 agtactagga gttctatttt tccattgata aatcgtgttt ttaggtatat ttaattcttc
    13681 ttccaattga gttatactta tgcctctttt atgagctaat tcctttattc tatctagtaa
    13741 attcattttc gcacctcaca agctacgaaa acactaataa aaaaattagt taaaggtgtt
    13801 gacaactaat aaaagtgtta gtatactgtt ttcgtaagct aaaatattag ctaaaaagac
    13861 ttgataacta ataaacactt cacggtcggc aaactctgaa atgtaaatta ctaggcattt
    13921 ctgtgtctta tttagctatg tctatatact aataaaaata ttagttattg tcaacgaata
    13981 ttagctaatt ttttagctta cagattattt ttttagaaag gagctatttt tatgtctgag
    14041 aatttagact taaaaattcg agcggagatg agaaaaagaa gaatgacttt caaagaacta
    14101 gctgcgctta tcggtatttc aggagcttat ttatcagata ttctaaacgg caatcgtgat
    14161 ggaaagaaag cacaacagca tatcgaaaca gtgaaaaaaa tattggacat ccgatagggg
    14221 ggtaggcgat gacaaaacta aaaaaacaag attttgtaaa aaaatacaat tattctccat
    14281 ctacttatca acgtcgaatg tcggaactaa aaaatacagc aattttctca gcggcgtatg
    14341 aacgggtcac aggacaagaa gtttggatca atacagaatt atacgataaa tttttgtctt
    14401 tcaaatccta taacaggtta cgcacaagaa aggtaacacc taaagaattt atcgagaagc
    14461 atttagttga tttataaaaa acagaacatt ttgagaggtg aaggttaatg ggtaaattca
    14521 acagagcatt ggtattcagc gcaccgctaa tcatctacgc tttaggactt tggggaagca
    14581 ggcaagcgtt gataggaacg attgtttaca tggtttggat ttttatgggg cttgatgaag
    14641 ctgaagctga gtacagagcg aaaaagccaa ccgagggggc tgactaaaaa tgaatagaaa
    14701 agagaaacta gaatgtatat tactattact cagtttaatt ctttcactaa tttctctatt
    14761 gggaagtttg tatttttgat atcaacaatt ttgtggattt gggttctatt tagagtaaat
    14821 atcatttttg tttggtgatt aaggagttta ttaaaattaa ttttccctac ttcaaatgca
    14881 aggtaacaag atatagcact tttaggagga atatttagcg gtacagcttc agagtatatt
    14941 ctattatcat cgactgatcc tcctttagca attaatttct taaatggagt agctgaccat
    15001 ctgcggttta agttagcgga tgaaagttct aaattaacta acatagctgg ttctgttgaa
    15061 tagttagaaa tgattacttt tgtataaatc atatctgcag cgaagtaaga agcattaagt
    15121 tcaacttcta gttgagggcg cattcctttt atttttatcc ctgttaaaaa cgtactcaat
    15181 ataaaactta ttattgacat ccattgaaaa attgttaagt gaaaaaaatt caaaataaac
    15241 accaccagtt tttaactaaa ttataccaaa aaggagagaa gaaataatgc aagaattagt
    15301 aattttgaaa aataaagaag ctgtgactac gagcttacaa gtggcagaaa gcttcgagaa
    15361 aaaacatcaa catgttttaa gagatattga tgcactaaaa aaagatgtgt ccaattttgg
    15421 acagatgttt gtagaaggta atgaaccaga ttcatatggc agaaatcgac gagttttctt
    15481 cattagtaga gatggatttt tcttgctggc tatggggttt acaggaaaga aagctatcta
    15541 cttcaaacaa aaatacattg aagcattcaa cgaaatggaa gatgttattc gcaagaatac
    15601 tgttcctcaa acaattgaag atatgatgat ctatcaatta gaagaaatga aagatgttaa
    15661 aaaagatgtt tccatgctta aagatactat gcgaattagc ggacaacaag agtttgaaat
    15721 taagcaaaaa ggaaatatga aagttatggg agttctaggg ggaaaagaaa gccgagctta
    15781 tgaagaaatc agcaaaaaag tattctcaaa attttggtct gaatttaaac gtaccttttc
    15841 aatcccaaga tatggcgagt tacctcgtaa gagattcgat gatgctgttt catttattga
    15901 aatgtggttg ccagaaactg cgatccgtat ggaaattgat caactgaaca gacaacagag
    15961 actttttggt gatgacaatg aatagagctg aagcgctaag aatagggacg gcaattgcta
    16021 attgctggtg gaaatactat aaaccaatca tcctaagcca acaacatatt gacaagcaaa
    16081 aagcatggca acaaataaaa aagtgactcc gccggcaagc aaagagtcac aaagaaaaca
    16141 catcataagg agattttagc atatggaaaa agaactttcc actctagatc aatatttgat
    16201 tgatcctgat tggggcaagc cgaaaattga ggaaacaagt ggtcgaaaaa tcagacgaaa
    16261 tcttttgacg aatgaagaac tagcttggga tcaagatgat ttaggcaacc atgtaactat
    16321 ttgggatcat gtttatctta tccatctatc gaagcattcg aataaacctg aatatattta
    16381 cgtcatcgaa gatggcttga ttgatgcgct agaagagtac gacagagata acttgattga
    16441 tatctcttat tacggaccag gtaagaaata cattgctgaa atggaggcag aatttgatga
    16501 gtgaaatcaa agggacaacg aactttgaaa aactttttag tcgtaagtta aataaaattc
    16561 tcaagaaaaa aggaaatttt gattatttat cttgggctca cgcgtgggag attatgaaaa
    16621 agaatgatcc acaggcaacg gtaactatta atgagtacaa acactacagg gttgtttctg
    16681 gaactcatca agactttctt gttgaggaat ataaaccttt tcttatggat gaaactggga
    16741 cttatgtatc tgtctcagta acggttaaag gacacacgga aaccga
//
LOCUS       DAJSAW010000058        16492 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJSAW010000058 DAJSAW010000000
VERSION     DAJSAW010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.058"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            1..1452
                     /locus_tag="ONT64_002183"
     CDS             1..1452
                     /locus_tag="ONT64_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR2303585.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1801..2433
                     /locus_tag="ONT64_002184"
     CDS             1801..2433
                     /locus_tag="ONT64_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HCR2303586.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3021..4460
                     /locus_tag="ONT64_002185"
     CDS             3021..4460
                     /locus_tag="ONT64_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2303587.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4755..4973
                     /locus_tag="ONT64_002186"
                     /pseudo
     CDS             4755..4973
                     /locus_tag="ONT64_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5222..5437
                     /locus_tag="ONT64_002187"
     CDS             5222..5437
                     /locus_tag="ONT64_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303588.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5654..5910)
                     /locus_tag="ONT64_002188"
                     /pseudo
     CDS             complement(5654..5910)
                     /locus_tag="ONT64_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6097..7008)
                     /locus_tag="ONT64_002189"
     CDS             complement(6097..7008)
                     /locus_tag="ONT64_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2303589.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7027..8031)
                     /locus_tag="ONT64_002190"
     CDS             complement(7027..8031)
                     /locus_tag="ONT64_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCR2303590.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8028..10151)
                     /locus_tag="ONT64_002191"
     CDS             complement(8028..10151)
                     /locus_tag="ONT64_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR2303591.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10156..12603)
                     /locus_tag="ONT64_002192"
     CDS             complement(10156..12603)
                     /locus_tag="ONT64_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2303592.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12590..12979)
                     /locus_tag="ONT64_002193"
     CDS             complement(12590..12979)
                     /locus_tag="ONT64_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2303593.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13039..13542)
                     /locus_tag="ONT64_002194"
     CDS             complement(13039..13542)
                     /locus_tag="ONT64_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR2303594.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13555..13779)
                     /locus_tag="ONT64_002195"
     CDS             complement(13555..13779)
                     /locus_tag="ONT64_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303595.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13883..15793)
                     /gene="ltrA"
                     /locus_tag="ONT64_002196"
     CDS             complement(13883..15793)
                     /gene="ltrA"
                     /locus_tag="ONT64_002196"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR2303596.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         4913 a         3271 c         2939 g         5369 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
      301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
      361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
      421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
      481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
      541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
      601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
      661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
      721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
      781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
      841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
      901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
      961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     1021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     1081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     1141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     1201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     1261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     1321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     1381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     1441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     1501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     1561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     1621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     1681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     1741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     1801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     1861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     1921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     1981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     2041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     2101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     2161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     2221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     2281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     2341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     2401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     2461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     2521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     2581 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     2641 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     2701 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac
     2761 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     2821 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     2881 ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct
     2941 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     3001 attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     3061 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     3121 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     3181 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     3241 ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     3301 gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac
     3361 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     3421 ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga
     3481 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     3541 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     3601 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     3661 ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc
     3721 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     3781 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     3841 tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt
     3901 atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc
     3961 ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg
     4021 agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt
     4081 tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca
     4141 atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact
     4201 tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt
     4261 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta
     4321 actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     4381 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     4441 gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg
     4501 gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc
     4561 aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact
     4621 gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct
     4681 actggttatg aatacggagc caacgctctc atctgcataa gaatacatca cccagattat
     4741 gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta
     4801 gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat
     4861 ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt
     4921 tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac
     4981 ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc
     5041 aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt
     5101 ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc tactgaaata
     5161 tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct
     5221 tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg attttacttc
     5281 caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg
     5341 tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat
     5401 gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc
     5461 cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca
     5521 tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt
     5581 agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc
     5641 ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt
     5701 tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa
     5761 ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac
     5821 atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa
     5881 aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga
     5941 ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg
     6001 aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact
     6061 acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca
     6121 gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt
     6181 atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta gataaactgg
     6241 attctctaac tccgaaaact ttaagtcaat attgattggt cgcatcgcac cattttcaac
     6301 ataatactcc aattcttttt cagaagctga tggataaagt ttaaaaaatg tttctaaaaa
     6361 atcggtgatt tcttccgttg ttttggcatc aatttctgaa tcgttttcta cttgttttgc
     6421 ttcatatttt gccttgtctg gctttccaat cattgttggc aaactagtta ctaaatgatt
     6481 cctgttttca tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt
     6541 tccctctgta atatgttgct ccactaaaaa tgaaacgaca aattcttccg agtgtttttc
     6601 aacatctaga atttttacag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat
     6661 atcttggatt aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt
     6721 ttcaattgct tctttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa
     6781 actttcaacg ccagaagtat tcactacctt ctcttttata accacttttt catgagttgt
     6841 atgttgatca atggcggtga aatttttata aacgccaaaa gcaagactac aagacaataa
     6901 aagccaaagg gcaagcacca tttttcgatg cttacccacg gatacaactc gttctttttt
     6961 cttttttggg ctcttttcct tttgattccg ttcaattttt atcttcattc tttttcctct
     7021 tttctcttat ttgtatcggc cagctccgat aagatgctgc cgccaatagt tttccgttag
     7081 attcgtccac cccactggat ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc
     7141 cacatgggtc acataagtac cggcatcata ggttgaatga aagaaaacta aatcacctgg
     7201 tttggcatct tttagatcaa tatgagtcat ggcatcatat tgatcctgag ccacccgagg
     7261 aagtttcagg tcaactttgg cataacacca ttgggtcaag ccactacaat caaaccccgt
     7321 ttctggactt gagccaccaa acacataggc cgttccttca tacttaaatg cctcatcaaa
     7381 aatttcttgg actattttgt catcaaactg agttgtagtt aggtattgct tcactaataa
     7441 acagtaaaac atattgccat aattataccg ccacccacca ttttccttaa tcgcgattgg
     7501 gttggggtaa tcgacttttt gaccacctga tttttccttt gaaaaagatt cagctaaagc
     7561 aaaactatgc ttcttcccgt ttttcccgac atgatctaga tacccgccac catagttata
     7621 ggcctgaata atactatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc
     7681 aaaatagaga cagccttgtt tgatggatgc ctcggtactt aatgaattag gtggtaatcc
     7741 ggctgactca gaactttgca taacgtcttc cattgtgcca ccagattcca cttgaataat
     7801 cgccaaaaga attggaatat actctgttat atccgattct ttggcatact tttccaccat
     7861 tgcacggtgt ttcaatactt cttctgagac actagttccg ccaggagaag tgccagaact
     7921 accatttccc tcactgtctt caccaaacaa caaacccaca cacatcaaaa tacccataaa
     7981 agagatggtc agaaaagtta atgaaataac acccactttc ttcaagttca ttttttctca
     8041 cctgaccttg attttggacg atggttcatt cttttttgta ctttttgatt ggtctgcttt
     8101 ggcagagtgg acctctgttt cacttgttgg aaagaatttt tcttacttgc tgattggctt
     8161 ttcccttttg aaatgatttc ttgactagtt agttttcgtt tgatttcggg attcagattt
     8221 gctctcacaa tccgctttgg tgcttctttt ttcagcatca tatctttctg aggttgagct
     8281 gctttatcta ttggaatatt cgcccttctt tttccctgac gatttgttgc ttcactcagt
     8341 atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc
     8401 actcgatccg ctttttcttg gaacagtccg cgcttgaatt cttctggtgc ttttttcgac
     8461 ttttcgattc ctttatggag attgtatttc aaattcgttg gcgtattcct tacctgatcc
     8521 tttacttgtt tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt gccgattttt
     8581 ctcccagcca aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtt
     8641 aagtcagata gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg
     8701 gatggcttaa atttggattt tgattgttcc tttgccaact tatttccgac tgctgcacca
     8761 ccaccaaaag ccaaagctcg tcccacattt cgattgagtt gccgcaattt tcgattcatt
     8821 aacatttgcg gttttcgcat gacgcgtctt cccattgact ggctatcatt actctggagg
     8881 ttgaacatac tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc
     8941 agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg
     9001 ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca tcataatcgt attgaataaa
     9061 cgaataatcg ccttctttcc taaactttca taagtcggca acatggacaa taagaaacta
     9121 attggcagaa acatcgcaaa gataatgaag agaatttgcg agaacaacat aatccctgtt
     9181 agaagaaaaa caaagaagga aatgccaata ttgaacaata ccaaaaagac cacggtccct
     9241 aaacggttca tggtcttggt aatactgaga ttcgcattgt cattgtcttc aatctctgcc
     9301 ttgaccgctt cttcccgatc tttgcctttg ttttcatctg gactcacaga tagaatcttg
     9361 ttgacccggt cttcaccaat ttcttctatg ttggaatcat caaattgcag tagtaaccaa
     9421 ggttgttgga cttgaatcga aaacaaacta tcccgaatca aatccacact atcttttcct
     9481 tggctttctg aattgggtac aacgatctcg gttccaaggg taagggccgc ttcactaaca
     9541 tctgagctaa agtcattgat ttttgtgata taggtaggcg cataagcaat gaacgaaccc
     9601 gatagtagaa aaatcatcac aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt
     9661 tttaataatc cggtatacgc cacatacacc cccagagcta aaatcatcaa taatagaaac
     9721 ccaaaataaa atccggaggt ctggagtcca ttttcagtaa taccagccaa tgtttggata
     9781 ttctttccaa tactttcagc cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc
     9841 acataacccg tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca
     9901 taaagaccat acatcacgct tttccctaag ccatcacccc aattccaagg caaccaatcc
     9961 caagagctat ccacaaagaa atctagttga tagttgttca gtgaatattt ggaatataga
    10021 tttcctgctt caactgtatc atcaactagc cctgtcgcct cggctaccgt accaactaaa
    10081 ctcaatagaa ctatcagagt gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt
    10141 ttctttttca tcccctcact ccattctctc ttgaacaggt ggacgggtat caaaggcatg
    10201 gaagagttct tcaaagactg gatgtacctg aaccacgcct acccgaccat acaaatcctg
    10261 catcaagcat tggccatttt ccaaatctcg taggcgtttt tgattgcttt catcttctcg
    10321 atccaagcca aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaacgc
    10381 aaacttcaac ccgatattat tcttcaagcg ttcatccagt aaatcatcac tattttgcgt
    10441 gacaaagtaa accccggcat tcatagcccg cccagcacga acgagcttat tcgataatgt
    10501 tttcccttgc gctacttgaa gaaaactcca cgcctcatct aagtctacaa ttttgaagat
    10561 ttctcgatta ctatggataa agtccaaggc aaaagtggaa atgacgatta gcatggaaac
    10621 actaagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa
    10681 atccgctact tgaatgatat ttaactgcct atctaaacta atcgactggg taatcgaacc
    10741 atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat
    10801 attttccgca actggtgagc cttcttttcg aagctcatta attacttgta ataatccccg
    10861 ttgattgctt tgggtaaccg tccgaatggc cttacgcaac actggaaact tctccccatc
    10921 tcgactagaa attcctgtta aaaaggtcag aatatcaatt gctagacttt cagaatcctt
    10981 ctttttctcc ataatcacat aaggatcaag taatccttga ttttttggct tactagttaa
    11041 gttgagaata ttgatttcat ccgcaatttc tggcaaggtt tctttccagc caccccgttc
    11101 cccttttgga tcgacaatca cagcttgtcc accaaaaagg acggcgtaat aaactaagag
    11161 attgttacta aaggactttc cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt
    11221 ggtaactgaa tccttgaccc cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc
    11281 cgtatctacg ttataaccaa agtaaatgcc ttccaactcc cctaacatct gagtggcgcc
    11341 aaaaccaagt ccggcgagaa aatcagacgt aacatactgg atataatcat tcatgtatcg
    11401 cttactagaa ggtaagaatt cttcatgtag ccccatcata tcgccaaaag gccggactag
    11461 ttttacattg gtatcatcat aaaaatctag cacttcatca caccggcgct ttagttcatc
    11521 tactgattcg gcacttactc gaaccacata gctcagtttg tacatggatt ctttggactg
    11581 atccaaggta gtttccaatt catctacgga atccaatgca tctaagacat tggaatttgt
    11641 ttcattgtca gattgatagg catggttgtc caagtctttt aattcctttt tcttattgcg
    11701 aacggtagca agtgcctttt tgttcgtcac aatctccaca ttcatactag tgtcaattgg
    11761 gaatgtaaat tgttgttgct ggtaatagaa aagttctgag gagggaaact ccatttcccc
    11821 cacaattgta ttaattgtta agtacgctac atatgattca tgattctcat gttccatgca
    11881 taagtaccgg ggcttttctt caatcaaaca acgactggga cgtaataagt cgtaacgttt
    11941 caccaaagtt tccgacttca gctttttctt tggtagctga aattcatatt cttcaaatgg
    12001 tgtacccttt tcgccataga tatgttcaat caaatacatc agatcactcg gtgtgactcg
    12061 tctgacttta aatcttcgag ccaatttact ttccattagt ttttcaagct tggtatatcg
    12121 gcgaatttct tcattggata aggaaacaaa atcgcccatc aaatgatgat tcactccata
    12181 gacaaattct tgaaagccgg agaagaaaga tttcttgaga ttctttagat tgacctcttc
    12241 atctgtcgca atcagtttga agccaataaa aaagcgataa tcaatctgac tatcgccaat
    12301 catggatact aatgcctctg tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg
    12361 cccggtaatt tccttttttg atcgttcttg cgtggcacga atactacttt ctgtggcaat
    12421 ttgtaaggca tgaatcttgc cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg
    12481 gacttgatat ttctgttcag gtgacaaaaa ggagtaattg taaggtatta actcatagta
    12541 ggcaaaacat tctccctctt gattaaagac aagattattt tcaatatact taatcgggta
    12601 catagttcac actccttacc agagtaatgg gttcgtccca tcgtttcttc tgttctttga
    12661 ctttctttcc tgcgaaagtg accttaaatc gtaagtgata gctaataaaa gatcgtaaaa
    12721 atcccaatgg tttcttaccg tcaaacgttt tttgcgacat gaaccaagta actgccaccg
    12781 gtatacctaa gtattttaaa aaggccccct ctatcataga tagtggaggc acatgggcaa
    12841 atacgatgac aaacagcaag gataaaacaa accaagccat ctggttaaaa gtcacaggaa
    12901 aaggcagttg aaagtcatta attgcataaa tgaccttttc aactgcccaa atactggtgt
    12961 agctttttat tttcttcatc aaatcattcc tttaaaaaga aggatagttt agaaatcaac
    13021 tacccttcaa taattttgtt aatatggata ctcaaaaaca cctcgactcg ttaccaaata
    13081 attgcctcca atctccaagt ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg
    13141 ttctggtact tctccaaaat caccagcttc tacaatcaag aaatatgcta catcactcat
    13201 atcatcgcaa tctggataat agataatatc atccacatgc tctaccatct cctcaaaaga
    13261 attgaagaaa gcatgttgaa tctcagaagc aacttcacca actggcgttc cttcaagttc
    13321 ctccgctaag ccacacaacc gattaatttc ctcaattggc gtatactcat caatctcaaa
    13381 gggaagttca taatcatgaa ttgcatattc ctcgtattcg tcatttaacc caattcgttc
    13441 tttcacttca tcaaaatcga caggtggggt aaaccacgcc cccaccaatt caccttcgtt
    13501 atatttcccg aggttcgcaa tataaacacg catctgttcc atcgtgcttc ccccttacgc
    13561 accgacaatt cggttaaaca aactgagtaa gacatccttc accccagaag cattaaagac
    13621 caatccgacc gcaatcaaag caatcaccaa aaagccaatc agtttggaaa actcccgctt
    13681 aaatcctaaa aacaaaccaa tgaccacaat tgccagtaag actaaggatt gggcattggt
    13741 taaaaaccat tgatatatgg ataggtaagt tcctgatatt ataaatatct caaacttttc
    13801 cccccattca cacggagcgt gcgactttca ccgcactccg cgttccatcg tgtttatatt
    13861 gaaaagtatt tatgtagtag acctatatca agtttaattc ttgattgcca gctaatactc
    13921 tatatttatt tagtcttatg agctggactt tatttagctt caatagagct atatattttt
    13981 gtattgtttc ttctgtttta gcgtgtatta gacggtgtac cgcctctttt accaaaataa
    14041 gatttttata atcgtcattt ccaccagctc cagttggtac aatatgatga caatgtattt
    14101 cattagcttg ttgaaattct tccagagtta tagcacattt tccatgttga gcagaaaata
    14161 gcgaaattcg attgtctgcg tattctattg tgcgattttc caaagataat ttcattaact
    14221 ggtacatcag taatttatca acacttagat tggtgtggat aaattttctt ccctctagag
    14281 tgtagcaatt cgctaaatac acctttgcca ttggttttct gtgttggata tatccaacag
    14341 ggaaaatagg ttcgccctcg ccagcaacat accgaagcat tttagattta ccatatcttg
    14401 ttgcctcctt tgtcgtaagt tgtctgcctg tttttctcaa tctgcccctt ttgtttgtac
    14461 ctaatctgtt agtgaagata gccattacag ctctattcag tttcatacaa tccagattta
    14521 cattcgttgc aatctgataa taattttgca tacctaatac cattgaatta taaagtcttg
    14581 tttcttctaa ttccgttttc ccttttgagg gagaagcaat tttctttgcc tgtgatacca
    14641 gcttgtcttt ttggtttttg aattgtttat cacttatatg tgatttaaca actaacttct
    14701 ttcttctcgg tatcatttta atcttgaaac caagaaagtc ggagtatctt tttctaacat
    14761 ttacaattct tgttttttct tgtgatactt ccaattttaa tcgttgttca atccattgag
    14821 tgattgctat tttggctttt tcagcacttt ccttgtagcg gcagaaaact ctaaaatcat
    14881 ctgcataacg aaccatatac atttccttta actttgtttt cttcatttct ttgtaagcgt
    14941 tgcttttaca tgggctaccg cttttattga tatgaaccac atagtttttt gtaacaggat
    15001 tttcctgcca ttggctttct acccaatggt ctaactcatt aagaacaatg tttgctagta
    15061 gtggggatat tattccccct tgcggagttc ctttctctgg atatgttatt gtaccgttag
    15121 tcattttgat tggagctttt aatatccttt ttaaaatgaa tatcaagtgc ttgtcccgta
    15181 ttcccatagc ccatatctgt tttatcagct tggaatggtt cacgttgtca aagaaaccct
    15241 taatatcaaa ttcaatcaca taatgtagct ggctgatttg taggcggtta taggttgctt
    15301 ttattgcatt ttcaactgac ctattaggtc taaaaccata gctgttttca ctaaatttag
    15361 cttcgcagat tggttctaat acttgtttta tacattgttg tataagcctg tcccacatac
    15421 aaggaatacc tagaggtctg gttttaccat ttggttttgg tatttctttt cgcctcactg
    15481 gcttaggtcg atacccatgt ggactgcctg caacaatgta tcttactttt ttgacaactg
    15541 tttctgctgg caatttgcca atatctttaa ttattgtacc gtctgttcct gctgttttac
    15601 tgcctgcatt tgctttgata tttcgatatg ccagtaatat attttctctc gataggataa
    15661 tatccataag atgagtgaaa atatctccat tcttactttt ggcatataaa tcatcaaagg
    15721 tgttttgcat ttcgtaatat tcagcgtgtc ttaaatcttc tacacacaat gtttcatttt
    15781 tatttttagg cataaggctt cactccttta cttgaaattg agtgagcttt tttatcttac
    15841 tcgtaatcct tattgaaaca acataatact tttctaatat atacacgact tgagggcatt
    15901 cctccattct cattacaaga atttcttagg ttcgccctct gcttttcagc attagttcaa
    15961 ctgcttattg ttcttcgtca gtttattctc ttgatgagaa cccaatacct ttcctcgttc
    16021 cgataatctt atctatacat atatatcctt aggtcactag ctataagcct gctggcttga
    16081 taccgcctgt aacgttatat ggcatttcat aacctatatt cttactttgc cacaccagct
    16141 acgatttctc gtacctttgc atttctacaa agttcaaatt tagacccgta cattcgctgt
    16201 ttcgtcagtt ccttatagaa cattctaacc atagatattt tatagacccc cgacctgttg
    16261 gatacgctac ccacctctgg atagttttcg atagaagata tatgttcatc aactattacg
    16321 gtatctttca gccgtcttta ccgagcttca aacattctga gtttcctcat acaatgcttg
    16381 tcggaggctc agggcaggcg tttcagagcg ttacttcatc attccacctg tcagattatc
    16441 agttctccta ataaccaaac tttttcgttc ttttattagt gccttagctt tt
//
LOCUS       DAJSAW010000059        16412 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJSAW010000059 DAJSAW010000000
VERSION     DAJSAW010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.059"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(152..550)
                     /locus_tag="ONT64_002197"
     CDS             complement(152..550)
                     /locus_tag="ONT64_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR2303597.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="ONT64_002198"
     CDS             complement(547..1320)
                     /locus_tag="ONT64_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCR2303598.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="ONT64_002199"
     CDS             complement(1317..2000)
                     /locus_tag="ONT64_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCR2303599.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="ONT64_002200"
     CDS             complement(2167..3054)
                     /locus_tag="ONT64_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2303600.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="ONT64_002201"
     CDS             complement(3051..3920)
                     /locus_tag="ONT64_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2303601.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="ONT64_002202"
     CDS             complement(4057..5343)
                     /locus_tag="ONT64_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2303602.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="ONT64_002203"
     CDS             5616..6194
                     /locus_tag="ONT64_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR2303603.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="ONT64_002204"
     CDS             6194..7123
                     /locus_tag="ONT64_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2303604.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="ONT64_002205"
     CDS             7120..8856
                     /locus_tag="ONT64_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR2303605.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ONT64_002206"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ONT64_002206"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCR2303606.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="ONT64_002207"
     CDS             complement(9752..10261)
                     /locus_tag="ONT64_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCR2303607.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ONT64_002208"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ONT64_002208"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR2303608.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="ONT64_002209"
     CDS             complement(11562..12653)
                     /locus_tag="ONT64_002209"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCR2303609.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ONT64_002210"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ONT64_002210"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR2303610.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ONT64_002211"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ONT64_002211"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR2303611.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ONT64_002212"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ONT64_002212"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCR2303612.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4743 a         3625 c         2915 g         5129 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tacaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccgc tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa at
//
LOCUS       DAJSAW010000060        16132 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJSAW010000060 DAJSAW010000000
VERSION     DAJSAW010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.060"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1230
                     /locus_tag="ONT64_002213"
     CDS             <1..1230
                     /locus_tag="ONT64_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR2303613.1"
                     /translation="LFFDEEWLIEKEYKGVQAQFIKGKLDDSPSYCEHCRSVRIIRNG
                     SYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILME
                     LYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVLCMDEFKSMKSVSGSM
                     SFICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLVMDMNASYCQLLKTVF
                     PNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTT
                     WEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETL
                     KTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNF
                     YNFRARIYLQQGLIFEN"
     gene            complement(1613..2341)
                     /locus_tag="ONT64_002214"
     CDS             complement(1613..2341)
                     /locus_tag="ONT64_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCR2303614.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(2319..3893)
                     /locus_tag="ONT64_002215"
     CDS             complement(2319..3893)
                     /locus_tag="ONT64_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR2303615.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            4122..5060
                     /locus_tag="ONT64_002216"
     CDS             4122..5060
                     /locus_tag="ONT64_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2303616.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            5072..5983
                     /locus_tag="ONT64_002217"
     CDS             5072..5983
                     /locus_tag="ONT64_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2303617.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            6007..7479
                     /locus_tag="ONT64_002218"
     CDS             6007..7479
                     /locus_tag="ONT64_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR2303618.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            7530..8141
                     /locus_tag="ONT64_002219"
     CDS             7530..8141
                     /locus_tag="ONT64_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR2303619.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            8171..10084
                     /locus_tag="ONT64_002220"
     CDS             8171..10084
                     /locus_tag="ONT64_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HCR2303620.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            10212..13193
                     /locus_tag="ONT64_002221"
     CDS             10212..13193
                     /locus_tag="ONT64_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HCR2303621.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            13190..14851
                     /locus_tag="ONT64_002222"
     CDS             13190..14851
                     /locus_tag="ONT64_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HCR2303622.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            14891..15823
                     /locus_tag="ONT64_002223"
     CDS             14891..15823
                     /locus_tag="ONT64_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HCR2303623.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         5228 a         2609 c         3232 g         5063 t
ORIGIN      
        1 ttattttttg atgaagaatg gctgattgaa aaagaatata aaggcgttca agcgcagttt
       61 attaaaggaa aattggatga ctctccttcc tattgtgaac actgtcgatc ggttcgtatt
      121 atccgcaacg gttcctatac gacacgcact cagatattga aagtcaaaga aaagttaact
      181 attttagaat taaaacgtac ccgttttctc tgccatgatt gtggaaggac cttttctgcg
      241 aaaacggact tagttgatga acatcatcaa ctaacaaagg aactgaagca ggcaattctc
      301 atggaactgt acgaaaatca atcacgtaaa ctaatcgcta agaagtactt cgtctcggat
      361 gggacggtga cacggatctt gcgtgaagca acgaagcatt accagtcacg tatgaacttc
      421 cttccgacgg tgttgtgtat ggatgagttc aaatcaatga agtctgtttc tggttccatg
      481 agttttattt gtgttgatgg gacaacccat cagctattta cgatccttga agatcgcaga
      541 ctttacaaac taactcaata cttcctgcac tttcctagaa aagcccgttt aaaggtcaag
      601 tacctagtga tggacatgaa tgccagctat tgtcagttgc ttaaaacagt ctttccaaat
      661 gcggagatcg taactgaccg ctttcatatt gtccaacaca tcaatcgcag cttcaatcaa
      721 ttacgtgtcc agatcatgaa tcgttttcga acctcacact ctgaaaatca gaagaagtac
      781 cgtcggctca agcgatattg gaaactctta ctcaaagatt ccacgacatg ggagccactc
      841 aaacggcatt atcatcgact gtttaaacga ccaattagtc aaacagaaat cgttgatgag
      901 ctgttaagct acaacgaaga attgcgaact gcgtatcatt tttgtcaatt attacgctat
      961 tatttcgtta aacgaggtac ggaaggcttt caagaaaccc ttaaaacaat ttcatctacg
     1021 ttacctcagt cgtttaaaaa gaaattcacg atcttccatt gctaccaatc gggtatttca
     1081 aatgccttta aggtgtccca ctcaaacggc gtcactgagg ggctgaataa caagatcaag
     1141 ctgattaaac gaattgcgtt tggttatcgc aacttttata actttcgggc acggatctat
     1201 ttacaacaag gtctgatctt tgaaaactaa aaaaatatgc ggcgcacaga acgcgccgca
     1261 tatcggacta agttttattc aaatttgtct tcaccaacac gatttgacga agagcctatt
     1321 taaataacta catccattta ttctatcttc taaaatagcc tttttcattt cagttaaata
     1381 gttcagcgcc atttttgcac tcgacttgca tagtagtttt ttccatattc ctcaacctca
     1441 gcgggggttg aagacataag gaaaatccta tgcgttatgc tcaactggat ctgattaaat
     1501 attctaatgt atttttctct accatcatta aatggttaat agccaaaaat caccactttt
     1561 caatggctcc ttgttgtata agtataacta gaagatgaac acgtctgaga aattatagaa
     1621 gctttttacg gtaattactg gggctgtgtc cagtagtttt tttgaagaga acagaaaaat
     1681 agctcacgtg atctattcct actttttgac taatttcatc aatagataag aatgtgtctt
     1741 tcagtagagc acttgctctt ttgattcgaa agtctagcaa gtaatcatga aaggaataac
     1801 ctgtttcttt tttgaaggcc cgtcctaaat aagcgggatg gtaatggaat tgtgtagcta
     1861 catctttgag cgtaagcttt tcactataat tgttttgaac gtaatcaatc acttgttgaa
     1921 ttgactcatt aaaagaatca tcagttagag agttttgatt agctgctgat ttacgcattt
     1981 tttcttcttg tactaactta actaaataag gaaaatcaat gggtttcgtt atgtaacttt
     2041 taatagataa atcaatcccc ttttttgcat actgaaaatt ttggtgggca ctaatgacaa
     2101 tactagtaaa gtagcgattg gatgtttgtt gcaaagattc gattaattcg agtccagaaa
     2161 ttttccccat cgtaatatct gtgataagta aatcaaagcg ctggttttgc aggtttttaa
     2221 atgcttcgtc tccgtctgtg tagcagtgat cgataatgat gtcggggact ttcttcaaaa
     2281 gataatgcct tatgccttct acaattgtac aatcatcgtc aacaattgca attctcaaca
     2341 tgcatattcc cccctttttg tattttgata gtaatcgttg ttccgacgtg tagttggctt
     2401 tctataacaa aacgtgtatc tccaggatag agatgcttta tacgtcgaaa agaatttgag
     2461 atgccgatat tctcactata aggaatatca tcattaagtt tttgttgaat atgctccagc
     2521 ttgtcttggt ccattccttt cccattttca acgattttta acacataatg gttggttgtt
     2581 tcagttgcat agacagtcaa acgattttcc ttttttgatg aatcgaagcc gtgtttaata
     2641 taattttcaa caaatggctg taaagaaagt tttggtaaca ttaattgatc aatatgatca
     2701 atcagctgat agtcaaagtt atgtcgataa cgacgttgat gtagttgcaa ggagaggtcg
     2761 atattaatta attcctcttt caaatcaatt atgtcgggct gatatgtatt attgcgatac
     2821 aacttgctca ataaatatat actgtcgctg atttcttcgt catcattcaa tatggcagtc
     2881 attcgaataa attccaaatt attaaataga aaatgaggct cgatttgata ttgaagtgct
     2941 gcatactttg cttcttgctt caaccaatta ttttcataaa gtgttttttg atatgtagca
     3001 ttatctaaat aaaactgatt gatatgcata gcgatttctt tcaaatcacc gactttgttt
     3061 tcgatattga ttgacttgta gtctcctgta gatgaatgat ttaaggaagt aatgatttcc
     3121 tgatactgct ggatatagtt acgaaaactt ctatttaata attgatgaat agcaagaata
     3181 aacaaactca ttaataagaa aatcatgatt gccagaccta ataaggtttg tgacgaataa
     3241 tttggatttt catatacttg taatgtgtgg ttaccgatag ctatctgttg cgtttttttc
     3301 catttagaag gcgagatgtc ttcagataaa ttgacggaat caataccatt agacaaagaa
     3361 taattgtaat gtgttggaaa gagactcttt tttagcgcgt tgatcgaaac gtctagaatc
     3421 aatgtagcat cgggcatgtc ctcactaatg aaacttaatg aacgaataaa agaaattttt
     3481 tcgttttttg taggtgcgta ggcacttaag tatttttcgt aatctaatga gatagatata
     3541 tttttaactt catcatcttg cacaagtttt tttaagttat ctactaaata ggtaggggag
     3601 cgatcctttg acaaaatatc ttccatgtat tcagcaggtg atgagaggta gtagtcatta
     3661 aggttttctt ggaaatcata attttgatag atctctgtca cgataccgga gagaatacga
     3721 ttatatgttt ccatatagtg ggtgatttgc tgaatgtttt gcaggttttg atttttttct
     3781 ttttcaatca cattgatata ggaaaagaac aatgtgaaac aggcaagtaa agtaataaaa
     3841 aaaatcaata tgtatgtaaa acgcataata acttttgata aatcgttttt cattcaaagt
     3901 cctcattttg ataatctcta attttatgaa tcattatagc acatattaca agtgatgtaa
     3961 acgctttgca aacagggcta cttatgattg tgaatacagt ttccgtttaa aaactattct
     4021 attgtagagg tttaataaac taaaaataat gtataaaaaa ttaatgtaag cgattaacca
     4081 agtgggtata atcaaggaaa cgctgagaga ggagagacaa aatgaagcta aagaaagggt
     4141 tattaggaaa tctaaaaaaa aaccgagcat tgtttattat gcttttacca ggtgcgttat
     4201 ggttatttat attttgctat ataccaatgt ttggagttgt aatcgctttt aaagatttca
     4261 gaatttcatc aaggggattc tttacaagtt tgttcaacag tccttgggtt ggctgggaaa
     4321 atttcaaata tctctttgct tcgcaagatg cttggatcat tactcgcaat acggttgttt
     4381 ataacgttgt aggtattttg ctaagtactt tttgtacagt gtcattagca atcattctga
     4441 gccaaatggc gaacaaacgg ttattgaaaa agatgcagac atttatgatt tttcctaatt
     4501 ttttatcatg ggtagtcatc agttactttg tttacgcatt tttgagtacg gataaaggat
     4561 taattaatat tttactaagc aattttggtg tggaacccat aaattggtat agtgaaccga
     4621 agtactggcc ttttattatt gtttttacga gtttatggaa aggcgtaggc tatggatcca
     4681 tcatttatta cgcgtcaatt atggggatcg atactagtta ttatgaagct gctttagttg
     4741 atggtgccag taaatggcaa cagataagga atgtagtctt gccacattta agatcgatta
     4801 ttgtcattat gttgattttg tctgttggta gtatttttcg agctgatttt ggattgtttt
     4861 atcaattgcc aagaaattcg ggggctcttt atgatgtgac gaatgtatta gatacttatg
     4921 tctaccgtgg attaatgaat agcggagata tcggaatgtc tacggcagcg ggattatatc
     4981 agtcatcagt tggctttgtc ttgattttga ttactaataa gatagtcaac aagttagatc
     5041 ctagttcagc actattttag gaggaatcaa aatgatatca aaattaaaac ggcagatgta
     5101 cttttcgctg ccagtcgaac ggtttttcac aatattacta atcttattta ctcttagctg
     5161 tatttttccc tttattttcg taatcgtcat ttcattaaca gatgaaaaag cattacaact
     5221 caacggttac gaattattgc cggctcaatg gagcactgat gcatatcaat acttaataca
     5281 agatggcgga cagttacttc ggtcattagg cgttacaatt atgattacag tgattggaac
     5341 gttgattact gtttttatga cgggaaccta tgcttatgtt ttatcaagag cctctttccc
     5401 ttaccggaag ttctttactt tttatctttt ttttaccatg ttatttgcgg gagggatggt
     5461 tccttcttac ttagtgatga cgaagatgct tggcttaaaa aatacaattt gggctttgat
     5521 tctacctctt gcttttagcc catacaatgt cattatttta aggacattct ttaagaagtc
     5581 gattccagaa tcgattatcg aatccgctaa aatggatggc tgtagtgagt ttcgagtttt
     5641 tttccaaatc gttttgcctt tagcgatccc aggggttgca acaataggtt tattttcaag
     5701 cttaggttat tggaatgact ggttcaacgc attgctgtat attgacacca acaaattgat
     5761 tccattgcaa tatttgttaa tgaagattca aaattcaatg gaattcttag caaataataa
     5821 tgacattaca ttagcgcagc aacaagcaat tcaaaatagc cttcctcaag aatcaacacg
     5881 aatggcaatg gtcgtagtag ctactttacc gattgcaatt gtttatccgt tcttccaaaa
     5941 atactttgtt caaggattga cgatcggtgg agtgaaagaa taaaaaagaa taggagatgt
     6001 taaaagatga aaaagaacat gcagaaatat ttgattggtg gtgcttctgt actaaccctt
     6061 ggcctattag gtgcttgtgg tagcagtaat ggcgaagcaa ataatgaaac aggaaccaca
     6121 aaagttactt ggtatcaggt tggagaagaa cccaaagatt ttgatgaggt catgacaaaa
     6181 gtcaacgaaa aattaaagga agaaatcaat gtagagttgg atatgcggct gattccggac
     6241 ggggattatc agcaaaagct tggtgtgatg atcaattcag gagaagaata tgatatttgt
     6301 ttcgtcaatg gcacagatta tgtgaattat gggaacaaag gtgctttcat tagtctcaac
     6361 gatcatttgg ataaagagtt gaaagattat gcagctgaat tgaatgaagg atttattgaa
     6421 ggtggggcaa ttaatggcga aacttatgga ttgccagtca ataacgatat tgccacaacg
     6481 gaatattgga cctttaataa agatttggta gatcaatacc aattggatgt agagaatgtt
     6541 gaaggtgtag aaacattggc tccagtatta gaaaagtttg cagaaaaaaa tactgatacg
     6601 aatattacgc caatcggtat gacccgtacc agtgtctgta ttccttatga caatattatc
     6661 ggtggtggtg taccgctagc ggttgcattt gaaggcgata cacaaacagt tgttaatact
     6721 tatgagactc cagaaatgat gacttatttg aaggctatgc atgagtttta taaaaaagga
     6781 tatattttga aagatgcagc cactgttacg acagatccat ttcgaattga tagtaaaaac
     6841 tggtttttag gtcgtaactc aagtggttat caaccattag cacaaaaaaa attagaagct
     6901 ctagcgggac atggattaat tttaaaacca gctggaaaag cttttaaaac gactggattt
     6961 gcaagagtgg caatgcatgc ggtatctgct tcaagtaaac atccggaagc agccttgaaa
     7021 ttgttgaatt atgtgaatac caatcaagaa gtagcggatt taatggttta tggaattgaa
     7081 ggaaaacact atgatcgcaa tgaagatggt tcgattactt taacaaactc tgatgattat
     7141 tatcaaggcg cttgggcatt ggtaaatact tatctagttt caaaaacttc accgattaca
     7201 gaagaaagta aaaaactaga agaagaaagt aaaaagtttc aaaatgaagc accgatgtct
     7261 cctattttag gtttctcttt tgacgcttct gattttcgaa cagaaattgc aagtgtacaa
     7321 aatatcgtgt cacaatatga agtactttta agctcgggaa cagttgaccc tgaacaatat
     7381 gtaccagagt tgttagaaaa actagaaaaa gctggattgg ataaaattca ggcatctgct
     7441 caagaacaat acgatgaatg gcgaaaatct aacaaataat tttttactaa atgaaaagaa
     7501 tagactaagc agttagtggg gggattcaaa tgaacgaaaa caaatggacc aaaggattta
     7561 attgggtttt tcaattgttg ttactaaact ttacttattt tatactaatt tgtttaggat
     7621 ttggaatttt aggttttttc cctgctacga tagctttgat tgctggcgtt gtgaatcgaa
     7681 aaaaatatgg cttgcaagag tctgtgaagt tcatgagtaa aatctataag aaaaattttg
     7741 ttagatataa tctatattgc tggtcagcaa ctttgttgtt ggccggcaat attactacga
     7801 tctggctttg ttggcagcaa agcgcaccat tgttttattt attaagtaca ttattatcgg
     7861 taattacact gttcctagtc tttgcaatgt tgaattgggt cgtgatcaaa agtcttgggt
     7921 tgtggatgat gggagaccga ctgattgact atttgctttt cacagcaacg atgttgccaa
     7981 cggtagtcat gcaaggcttg gtgcttggaa tttatttccg aatattgatg attttgcctg
     8041 tgatttttct gtttggcgga acagctgtaa cggtaatatt gattagtaaa ttaactgaaa
     8101 gagcgttagc aaagttgcct tgtcagatac aatcgcacta attttttcac aaaaaggagt
     8161 atttctttct atgaagttaa ctgataaaca agtagctaat atagccttta tccagcaact
     8221 tgagattcca ttgacacaaa aagatgaaga acaaatagac ttgattttta ctgacacaaa
     8281 ggctaatgtg cgacaaatca agattactaa aaatgagcag caacgaattt gcttgtctta
     8341 ttcacatgaa gacttgctgt atcgaggatt gtttatctgc cttccacgtt ttttagctca
     8401 aggaggagag atgccataca ttgaagaaac ctcgatcatt gagaccagtt attcattaga
     8461 tgcttctcgt aatggcgtga tgaataaaga gactctaaaa aaattcattt cttacttagc
     8521 gttgatgggg tacaacacat tgtatattta cacagaagat acctatgaag tagaagacct
     8581 tccatatttt ggttaccttc gcggagcgta tacaaaagaa gaagtgaatg aaattgttga
     8641 atttgcttct ggttttggca tagaagtagt tccatcgatc cagacccttg ctcacttgac
     8701 ccaatttttg aagtggtatg ctcaacaaga actgatggat gatcaaaata ctctactgat
     8761 cggtgaagaa aaggtttatg cggcaatcga aaagatgatt attgcctgca aagaaatgta
     8821 tcagacagag cgtattcatt tgggaatgga tgaagcctat caagcaggtc tgggacgata
     8881 tttaagcctg tatgggtatt gtgatcggac aactttaatg attcaacata tggaccgtgt
     8941 gttatcaatt gttgataaat ataagatgaa accaatgatt tggagtgatt tcatttataa
     9001 gctattagat gaaaccggta aagataggta ttacaatcct gattttaaac tcaaagcatt
     9061 agcagctaaa ggattaccag caggattaac ctatgttcat tgggactatg gttcagatga
     9121 agtttcctcc tatgagaaag taattgataa ccatttgaaa ttttgtgaca ttgatcatta
     9181 cgttttagct agtggtgccc atatctgggg gagactagcc ccgaatcatg ggaaaacaga
     9241 agcaattgtt tctgcagctg taaaagcttc taaaaataaa ggtgtacgtt ctgtgatgtt
     9301 aactacttgg ggagatgaag ggcaggaaac agagcattgg cattccttaa ttggggcatt
     9361 aaaattagca gattatgtct attctgaaga agagtggaat gattttgagg aaatctacga
     9421 tcgtttatta ggaacaggta gttatcaatt aatgtccgaa ttacgatatt tcgatgaagt
     9481 ggataccatt acaaataaca acttacaaat ggcttccgtg agtaaactgc tactttggca
     9541 agatccgtta gttggtatat atgactttca tgtagccgct cataacaaaa ttgccactag
     9601 aacaattggt acttattatc atgagttagc tcagaaatta gatatgaata aaaattggaa
     9661 tccaaaacaa ccaattttaa caactattcg tcaacgatat atacttttag ctgaaatttt
     9721 agcacaaaag gcagaactag gcatctattt acaacactgc tgtctggtag aagaccttgc
     9781 aggattaatg gaattagcga atcggtgtga agcactagcc aatcgagtaa atgaattggc
     9841 atctattcat gaaataattt ggtatcaaac atacaaaacc tatggttggg aagtattaga
     9901 acaacgatat gctgcattag ctcatcgtct gaaaaccgtt gcgcgtcgta ttaaaaatca
     9961 tcttcagggg caagatgccc tagaggagct tttggaagaa cgattaccgt tttgtcaata
    10021 tcaagtgccg cttgaaattt ctggctttaa ttatcggcaa agctcggtgt caggctacaa
    10081 ttaacgaagg aatcttcccg ttgaacggac cacttgctca tctaatggga aagtggttcg
    10141 ttttctattc ataagtagct taccactaac tcttttatta agaagagaaa aaaagtagga
    10201 agtgagaaaa tatgttatcg gactattatc aaaatctgtc agttataagt gtcaatacag
    10261 aaccattacg taattattat attccttatg caagtaaaga agaagcaaaa caagagttag
    10321 aaagggaaaa aacgagccag tttatcgatc tcaatggtac atgggatttt cagtattatc
    10381 cttctgtcca acagttaccg gaaaattttt ttcaagaggc ctttactgaa agctatgata
    10441 gaatttctgt tcctggagtt tggcaaacag agggatttga cttacctcag tatatcaatg
    10501 tatcttatcc aattcctttt gatccgcctt atgtacctaa tgatacccct tgtggtgtat
    10561 atcggcgtca gtttcaaatt gagcaaacag atgcacgctt ttacttaaat ctagaaggag
    10621 cagatagtaa ttgttttgtt tggataaata gaaactttgt aggctatgac caagtgagtc
    10681 atagtacatc tgaatttgat gtgaccaagt atttacaggt tggggttaat gaaattcaaa
    10741 ttctagtgat gaagtggtgt gatggtacct actttgaaga tcaggataag tggcgaatga
    10801 gcggtctatt tcgtccagtg tatcttcttc aacgaccaac tatgcatctg acaagttatc
    10861 aactacggac gttgactgat cctaaaaaag gaagcggaac attaatggtt tcttttcaac
    10921 gatttactcc tattgaagta tcgttaccta tttcttacga attattccat cctgatggac
    10981 gtctcatagc gaaaggggag tgttactcgg aacaacaaat aaatttggat gttgaagatt
    11041 gccttttttg gacagcagaa acgcctaaat tatatacatt acttttaaca tgtaatggtg
    11101 aatattttaa agaaaaagtt ggctttcgag cactgacgat caagaatcgt aagttattgc
    11161 tgaacggaac acctattaaa ttatatggag tcaactatca tgacgtgaat cagcaaggca
    11221 cgccagtgat gacccgagag cagttacgtg cagatttgtt attaatgaaa aaattaaatg
    11281 tgaatactgt acgaacggca cattacccta aatcaccaga tttttatgaa ttggcggatg
    11341 agattggtct gtatgtaatc aatgaagcag atttggagtg tcatggtgtg gttaatctat
    11401 atgatactga agcgaattat caactaattg ctaatgatcc gacattcgaa aagccgatta
    11461 tggaccgagt gatccgttct attcttcctt atcaaaatcg aagctctatc attatgtggt
    11521 ctatggggaa tgaatctggt tatggagtaa attttgaaaa agctcaagct ttagcacgga
    11581 aattggatga ttcacggttg attcataatg aacgctcgat tgaaccagtt attgaccaag
    11641 tgaatgattt tagtaacttg gatgttatta gtcaaatgta tcccccactg gaagatgttc
    11701 atcgtcattg tcaaagaagt gatactgaaa aacctttttt tatgtgtgaa tatagccacg
    11761 ctatgggcaa cggtcctgga gatcttaaaa cgtacttcga tttgattcaa cagtatgatt
    11821 cctttcttgg cggttgtgtc tgggagtgga ctgatcatgc aatcaataga ggaactttag
    11881 aaacgccatc ttatttatac ggtggtgatt ttggtgaaaa atatcacgac ggcaattttt
    11941 gcgtagatgg tttattgacc ccagatcggc aattaacagt aggtgcattg gagtaccgta
    12001 atattcattc gcctattcaa ctgattgctg tagatgagga gctgcaagaa gtaaccttga
    12061 gcaatataac tgcttttaca gccggttcag catttaacct ttctcttgaa tggtatatag
    12121 atggaaaaaa aacagtttat gaagtaatga atcctttcat acttgagcct ggcaaaagtt
    12181 gttcattgcc catcacagta cctacaacga ttcatggtgc tcatacggtt gctgtttcgc
    12241 tatctgttta taaaaatgat gagctttatg gacagcaaca gctctttctt aaaaaagaaa
    12301 aaatgagatt gccaagaact gctcatcata atgatgcacc acaaatttca gtaaaaggca
    12361 cagagctgat aatcaaagga gaatccttta tttatatata tcaattagca aaagctgcgt
    12421 tcacttctgt aaagatcaat caaaaggaac ttttggaacg tcctactcaa ttttcaattt
    12481 ggcgtgcacc aacggataat gatcgtaact tagtaaacaa atggaaagca gctggatatg
    12541 ctgaaatgtc tttagaaatc agaaaaaaag actggaaatt cgtagatgat catctcctga
    12601 tcgaaacgtc ttatcgagga gttattccag gattacagcc aatttttaca atgcacatac
    12661 agttgaaaat aaatggtgca ggcgtaatca accaaaaagt atcagtggag aaaaatccga
    12721 tttttccaga tttccctcgc tttgggatgg actggccgtt gaatgatact ttttcgaagg
    12781 tttcttattg tggctatggg ccgtatgaaa gttatcctga taagcactat gctgcgatgt
    12841 ttggtattta tgagatggag attgaggaat ttgatcagcc atatatcaaa ccacaagaat
    12901 atggtaaccg ctggcaatgt gattgggtat caatcaaaaa tcatcaagga tctaccatga
    12961 cggtttatgg aaactctaat ttttctgctc tgccttatag tcaagctcaa ttaaccgaaa
    13021 agtcacacca ttatgaatta ataaaagaaa atagaacttg gttcaattta agttataaac
    13081 aaaatggtat cggctcacac agttgcggtc cttatttaga agatcgtttc cgttttatgg
    13141 aagatacttt tgactggaat atagtgatta ctttcaaagg agaaaataga tgaaagaaac
    13201 aaagatcaat caagtcaaaa aaaaattatc tcgttatcaa gaagttgttt atcgaaacca
    13261 ttgcttaaaa tggtcaaaaa ataagctgaa tgttcgtttt aatggattat tttatcgttt
    13321 cgaacagtta gaagaagtct ggaaaccggt aacgaatttc caatcatgtc ctgtacagct
    13381 cataaatgat ccaaaagaaa catacgattt atgtataaca cacctttata accagcattt
    13441 gaaaaatgac ggattttatt taaaggttac taatgacaat caacttacga ttgaatcagc
    13501 aaaccttcga gggtttcaat atgctatgga agccttatta gagagttttt tttcggtagg
    13561 agatcaatta ttattaccgg aattgattct gaaacatgaa ccatcagtga aaattcgagg
    13621 aatcattgaa gggttttatg gtatcccctg gacacatgaa atgcgcctct ctcttttttc
    13681 atatatgaaa gataaccaat tgaatacttt tatgtatgcc cctaaagatg atgaattatt
    13741 acgtaaaaaa tggcgtgaac tctacgatgc gcaagaatta gacaaattta aggagctttt
    13801 atctgctgct gaggctagca atatcgattt ttggtactta attagtcctg gaaatgatat
    13861 tgatattacg tgtgaagaag acattcaagt tcttctgaaa aagttagaac aactgattga
    13921 gcttggcgta tttcaatttg gtctattaat ggatgatatt gactatcaat taaaaggtgc
    13981 tgctaaacgc cgttttcgag agccagcatt cgctcatgcc tatttagtga atcgagtaga
    14041 ggaatatctt agcactcggt tgtccaatca tgaattagtc atttgtccta cagaatatga
    14101 taatcgtcat ggttctcgct atttagcaac gttaagtcaa gaaataccag aacaattacc
    14161 gttcttttgg actggcccaa gcacgttagc ggcgtcaatt tctacagaag agctgcagga
    14221 aatggcttct gtctatcaac ggccgatgct gatttgggat aatattccgg taaatgatta
    14281 tttagaggat aaagaactat tgttcatgag tccatatgag aatcgaacac cgaatctttc
    14341 taaggagaga taccaggtta caggagtagt ttcgaatcca atggctcaat tagaagcttc
    14401 aaagtttacg atcaatagta tggctaatta cttatggaac tgtgagcgtt ttgatccttt
    14461 ggaaacttgg acgtcagtcg ttgaaaaagt agttggaagt aaactacaac cagcttatct
    14521 taccttaacc aatgcttttc caaatcatta cacaagcagt ctgctttcag atgaagaatt
    14581 actgctagcc aaagacccag aatgggtcac aaaagaaatg gctgctttag tccaagcagt
    14641 aaaatggatc cagcaagaac aacatacatc tagattgaga acagaattgg agccttggtt
    14701 acaagcaatc acagaaagtt ggactttctg gcaagaatgg caattaaaaa aagatccaaa
    14761 agaagcagaa gaatggttgg ctaagaaaaa aacacaccgc ataggacgtg atttagtaac
    14821 tgcccattta gagcaaatta tcaaaagtta gatatcttaa aaaataaaaa gcaagaaaaa
    14881 ggagaacaaa atggtcaaga gattaattag tgcaaactac agtgatgtca agaaaatgac
    14941 gtcaaaagag ttgaagcaat ccatcaaagc gagtgaagga cgggtgattg tttgtgaaac
    15001 ggttgctaat actcttccac aatctggaga aattacgaat gcagaagttg cgtcagcatt
    15061 tggtgcagat ttgattcttt taaatggttt cgattgttat cgtcctgcag tttttggact
    15121 accaaatgtg acgattgaga aggtttttga tctacagcaa acgattgaaa acccgattcc
    15181 taaattaaaa gaactagtcg gtcgtcctgt gggaattaat ttagaaccca ttgcccctaa
    15241 aacaaatatg atgagtgaga aagtagaaat cagccaagga agagcaagct ctgttgaaac
    15301 gatacaaaaa gcagaagaac taggagtcga ttttatttgc tttaccggta atccaggcac
    15361 tggagtaacc aatttagaaa ttactaaatc cgtaaaaatg gcaaaaaagt atttttcggg
    15421 attgattatt gctgggaaaa tgcatagcgc cggctcagaa gagccagttg tgaataaaga
    15481 ggcgattatt tcttatgcgg aagcaggcgc agatatttta ctagttcctt cagtagggtc
    15541 tatcccaggc tttttagagc aagacctaat tgaagcggtt cggttggcaa aaaaatatga
    15601 tttgttaacg atgtctgcaa tcggtactag tcaggaaagt gcccgaccag aaacattgcg
    15661 gcaattagcc attatgaata aaagttgtgg tgttgattta caacatattg gagatgctgg
    15721 atacggggga ttggcaccag cagaaaatat tttcgaaacc tcggttgcaa ttagaggaat
    15781 gcgacatacc atcaactgcg ttagccgctc gatcaatcgc taacaaaatt aaataaaaaa
    15841 tggttaaatt gattatttaa gtgggaacat acttgagata tcagctaact tctgtcactt
    15901 taaaaaaggg actgtgacag aagtgaaaat agacaaaaaa tatccgaaca agagactagg
    15961 atagcagaaa aactatcgtt ttgcaacgag gaccaactgg accgtaggag caattctctt
    16021 attgttcgga tatttcgctt gttcaaggac ttatgtctgt agtgcccttt tttggtagga
    16081 aaaatggtag ggaaccatat catttttaat gaaaatcaag catttcaaaa gt
//
LOCUS       DAJSAW010000061        15445 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJSAW010000061 DAJSAW010000000
VERSION     DAJSAW010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.061"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..562
                     /locus_tag="ONT64_002224"
     CDS             <1..562
                     /locus_tag="ONT64_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR2303624.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            762..1076
                     /locus_tag="ONT64_002225"
     CDS             762..1076
                     /locus_tag="ONT64_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2303625.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1097..1474
                     /locus_tag="ONT64_002226"
     CDS             1097..1474
                     /locus_tag="ONT64_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2303626.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1484..2257
                     /locus_tag="ONT64_002227"
     CDS             1484..2257
                     /locus_tag="ONT64_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303627.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2279..3682
                     /locus_tag="ONT64_002228"
     CDS             2279..3682
                     /locus_tag="ONT64_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCR2303628.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3864..5048
                     /locus_tag="ONT64_002229"
     CDS             3864..5048
                     /locus_tag="ONT64_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR2303629.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5045..5335
                     /locus_tag="ONT64_002230"
     CDS             5045..5335
                     /locus_tag="ONT64_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303630.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5332..5553
                     /locus_tag="ONT64_002231"
     CDS             5332..5553
                     /locus_tag="ONT64_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303631.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5595..6374
                     /locus_tag="ONT64_002232"
     CDS             5595..6374
                     /locus_tag="ONT64_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HCR2303632.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6441..6935
                     /locus_tag="ONT64_002233"
     CDS             6441..6935
                     /locus_tag="ONT64_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR2303633.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6991..7629
                     /locus_tag="ONT64_002234"
     CDS             6991..7629
                     /locus_tag="ONT64_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303634.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7701..8096
                     /locus_tag="ONT64_002235"
     CDS             7701..8096
                     /locus_tag="ONT64_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2303635.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8080..10533
                     /locus_tag="ONT64_002236"
     CDS             8080..10533
                     /locus_tag="ONT64_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2303636.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10530..12557
                     /locus_tag="ONT64_002237"
     CDS             10530..12557
                     /locus_tag="ONT64_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303637.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12554..13576
                     /locus_tag="ONT64_002238"
     CDS             12554..13576
                     /locus_tag="ONT64_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCR2303638.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13593..14522
                     /locus_tag="ONT64_002239"
     CDS             13593..14522
                     /locus_tag="ONT64_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2303639.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14767..14883
                     /locus_tag="ONT64_002240"
     CDS             14767..14883
                     /locus_tag="ONT64_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCR2303640.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14899..>15445
                     /locus_tag="ONT64_002241"
     CDS             14899..>15445
                     /locus_tag="ONT64_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR2303641.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGN"
BASE COUNT         5257 a         2564 c         3062 g         4562 t
ORIGIN      
        1 attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag atttattaca
       61 gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa
      121 atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat
      181 ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt
      241 atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac
      301 tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa
      361 cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc
      421 gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga
      481 ggtgggttat tttacgagta aaggagactt gctttatccc actcaattat tagataaaat
      541 agaatttgat tataatattt aaaataccta ggtagtcgtt gttttaaata tagtaggaat
      601 cggacgctca gtacggagcg tctatttttt tacccaattt tgaaaggaag tgaacttatg
      661 aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat cacacaaata
      721 agattcattc aaacataaaa acagaaagga taggtaaaaa tatggaactt aaatatgtca
      781 ttcccaatat ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg aacattgaac
      841 aacgtcgaat aaatggcaga ccaaccattc tctctcgtag ttacaattta tactcaacga
      901 ttcaacgagc agatgatatt gttgtcattc ttccctccga agctggcgag aaacattttg
      961 aatccgacga aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt tataaaattg
     1021 gcaatcgagg atttaccaat tacattatgc acgcagacga tatggtgaaa gcataagaaa
     1081 ggaaaggtga aaaaatatga gattagcaga aggtattgtg attgaaaaag accagacatt
     1141 tggcacattg aaattttccg ccttgcgtcg tgaggttcgt atccagaatg aagatggtac
     1201 ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat
     1261 gatacaagtc agtatccccg cttctgttcc tttgaaagaa tttgactaca acgcagaagt
     1321 ggaattaatc aatcctgtgg cagataccgt agcgacggct acttttcgag gggcagacgt
     1381 tgattggtac gtgaaagcag acgatcttgt attaaaagga cagacaaaac ctcatcagaa
     1441 atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa acacaccatt
     1501 gattttgaaa gaaattccga aggatgaagc catttctttt attcggcaat accattattc
     1561 aaagatactc cctagattgt gtaaatactt tctagggatt ttttcagaag aaaaattatt
     1621 aggcgttgtg gaattaggct ggggtacgca acccctacaa accatacgca aactctttcc
     1681 cgacagttcc cttcaaacca cagattattt agaaattgga aagatgtgtt ttcttccaga
     1741 aatgaatcaa accaattatt ttggcagtca agctctttca gctctaatta agtggttgaa
     1801 agaacatacg gactgtcatt ttttatatac actggcagat ggaattgagg gaaaatgtgg
     1861 ctatgtctat caagcgtcca acttctttta ctgcgggtat ttcaagacaa gtgtctatcg
     1921 tgataagcag tcatgggaaa agatacaccc tcgaagtgct cgccttttat tagaggaaaa
     1981 tgctcgcttt gaacaggtag agaaaaagca ttggctttcc caggcatttt gcgaatacaa
     2041 agggattgag aaaatcaatg gacgcatgtt tcgctatctc tatccgttga caaaagaagc
     2101 gaaaaaactg ttaggacaca ctctctatcg acgtcattat tatccgaaag aaaaaaattt
     2161 acgctttgaa aagcgaatcg cttatcagaa atatgaagcc atttctcaac caacttttga
     2221 taaacaggct cgtatctaca atacacaatt attttgatag gtggaaagga ggatttttat
     2281 gccattgctt catgacagag gagagcgaat caaagccaac gataagcact tggtctatca
     2341 ttttgtacta atttcattgc tggtagtttt tctagtcgtc ctactccctt ttcattggag
     2401 agcgttacaa tcggtgaact ggcaagcagt tcagttagat acatttttct atcaacttca
     2461 tacgccatac ttttggagca gtattctcac tgctcttttt gtatgcggac ttatcagctg
     2521 gctttattat cactatcgta tagattctat aaaacagtta gagcaccgtc aaaagttagc
     2581 tcgtatgatt ttagaaaatc attggtatga agccgaagaa actcaatcag agggattctt
     2641 taaagattta cccagcaaga aaaccaaaca acgaatccgc cattttccta aaatatatta
     2701 tcgaatgaaa gatggactaa tccatattca tgtagaaatc accttaggga aatatcaaga
     2761 tcagcttttg cacctagaaa acaaactaga aacaggattg tattgtgaat tagtggaaaa
     2821 aacgttgaaa gattcttatg tagagtatgt cctactttat gatactattg gcaatcgaat
     2881 tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg
     2941 ggagttcgat tctctccctc acatgttaat tgctgggggc actggtagtg gaaaatccta
     3001 ttttattctc acattgattg aatctctcct gcatacaaat gcaaagcttt atattcttga
     3061 ccctaaaaat gctgatttgg cagacttagg ggcggtcatg aataatgtct attaccgtaa
     3121 agaagatatg ttggcatgta tcaatcaatt ttacgaagat atgattgatc gcagtgaaac
     3181 catgaaacaa caccctaact ataaaacagg agaaaattat gcttatcttg gtttgtctgc
     3241 caattttctt atttttgatg aatatgtggc aatgatggat atgctaggtc gtgaaagtac
     3301 caccgttatt cataaactca aacaaattgt aatgttagga cgacaagcag gcttcttttt
     3361 aattcttgcc tgtcaacgtc cagacgcaaa atattttagt gatgggattc gggacaactt
     3421 taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag
     3481 cgatacacaa aaacaatttt tcttaaaacc catcaaggga agaggttacg tggatacagg
     3541 aaaaagtgtg attagtgagt tttatacacc ccttgtacca aaaaggtacg atttcttagg
     3601 agaaatagga aaagtcattc aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg
     3661 tacaggttca gacttgcttt gattggtgtg gcgttagcca cgccaatcct ttagccccct
     3721 ctatctaaca ggggggcaca aatacaactg aaaacaggca attctcatca accaatgttg
     3781 atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact caagggtcat agtttacatc
     3841 ttttttagat tggaggtttg cgaatggaaa acgaaaaaat ttggattaag gaattgaaag
     3901 aaaagcgact ggcttatggc gtttctcaaa ataaactggc ggttgcttct catattacca
     3961 gaccttacct atcggatatt gaaacaggca aggcagtccc tactcaacag gtgaaagaag
     4021 atttactgaa cgctttggaa cgcttcaatc ccgataaccc attggagatg ttattcgact
     4081 atgtacggat tcgattccct accaatgatg tggctcaaat tatcggtgag gttttacgtt
     4141 tgaatatgga ttatatgcta cacgaagact ttggctatta ttcttatccc gaacactatc
     4201 gtttcggtga tattgttgtc ctagtctctc atgatgtaag taaaggtgtt ttattagaac
     4261 tgaaaggaaa aggctgtcgg caatttgaaa actttctatt ggctcaacat cggagctggt
     4321 atgatttctt tcaagattgc atggaacata aaggtatttt caaacgcttg gatttagcta
     4381 tcaacgataa aacagggatt ttgaatattc cagatttggc aaagaaatgt aaacaggaag
     4441 aatgcatttc cgtctttcga agctttaaaa attatcgcag tggtgaattg gttcaccgtg
     4501 atgaaaaacc atacatggga aacacacttt atattggctc attaaaaagt gaagtctatt
     4561 tctgtatata tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg
     4621 cagaagtaaa gaatcggttt gaaattcggt taaaaaatga ccgagccacc caagccatga
     4681 aagatttatt ggcacatcaa caggcggaaa aaacagcctt tgaaattatc aatcgctata
     4741 tccgctttgc agataaagac gacactaaac gtcggagtga ttggaaaact aatgaacgtt
     4801 gggaatggtt tatcggtaaa aatcgtggag cattacgcct aaccacacag ccagagcctt
     4861 attcttttga acgaacattg aactggttac atcatcaagt cgcccctaca ttaaaaatcg
     4921 caagcatttt agatgttctc aacggtacaa ccattatctc caccatgatt caggaagcca
     4981 aactgacaga aaaacatgag aaattaattg aacaacaaca tctagccatg gaggatttga
     5041 ttacatgagc cttttaacaa aagaattaaa gaagttaggg tttcagtgtg gcattgagtt
     5101 tcaagcgtat atacaaaata cagggaaata tacgtctctc attattgagg gaaaacggca
     5161 agctggtgat accatctata cctatgattt ttacaaggtg agattttata ataattatac
     5221 caatcgagta accgtctatg gagagcattt aacccctttt caattattaa ggcgtgtaaa
     5281 aagctatatc tattatcgag aaaaatatct taaggaaagg aggacaatca catgaatttt
     5341 gggcagaatt tgtacaactg gttcttggat aatgcacagt cattggtatt gttagcgatt
     5401 gttgtcattg gactgttttt aggttttaaa cgagaatttt caaaactcat tggcttcttg
     5461 attattgctc taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta
     5521 ttaaacttat ttaaccgaat cattggtgct taacagatat ttttcatgat aaaataggaa
     5581 gtatatattt tgttatgaaa ggatttatga atatgtttac aaatgatcaa tttaaatctc
     5641 ttttaattaa aacattagaa agaaagagct cttcaaatta ttatgaagga ggtaacgaga
     5701 ttgtttctaa aatgaaaata tcagaggtaa ctttagatga gactgacgat tttacttatt
     5761 ataaaggata taaaaagggc tggaatacgc tttgcacata tcttaaaata agagttcctt
     5821 ttgatgattt agactttttt gaaagtcata aagatttgat tacccagaca gcaagctcca
     5881 tatatgataa acaaggtgat aatgtattag tggatactat tttagtacca cttccagaga
     5941 attacgaagt aattaacttt agtcagttga aaattagtga tgtggtttca caagctattg
     6001 aagacgctga gtcttttatg agtaatggtg aatatcaacg tgcctttgac agagttcaca
     6061 ctgcttttca tggatattta attgaaatac taaaaaaata tgaaattaca gttcctagag
     6121 acgaaaatct atctaaatta tatagtagga tacaacaatt aattgaaaaa gaaattcaac
     6181 ctactgaact tgctgatata gtgaagacaa cgataaggtc atcaaatgga atgattagct
     6241 ctcttaatga agcaagaaat aggcattcac ttgcacaccc taatacaaat attattggaa
     6301 agagagaagc aaaactaata ataggtattt caagtacagt tacagattat attagcggat
     6361 accttgataa gtagcgtcat taactgattg ctactttttt tatgactatt tttataaaaa
     6421 ggatgtgata agaattggaa atgaaagtct atgtggcaaa tttaggacgg tataatgaag
     6481 gtgaattagt cggagcatgg tttactccac caattgatga ggaagaaatg gcagaaagaa
     6541 tcggtttaaa tgaggactac gaagaatatg caatacatga ttttgagctt ccttttgatg
     6601 tggacgaata cacgccgatt agtgaaatca accgtctatg tgaagccatt caagaaatcg
     6661 aaggtacacc tatttataat gaactcaaag aaattcaagg catgtggttt agtagcttgg
     6721 aagaattact ggaaaacaaa gaggatattc attgttatag tgattgtgat tcgatggaag
     6781 atgtcgcccg ttattacgtg gaagaaacag gacagcttgg tgaagtccct tccaatttac
     6841 aaaattatat tgactaccaa tctttgggac gtgatatgga aatagagggt aattacctag
     6901 ttacctctca tggggtattt gaatattgtc agtaagttta acaatccgtg ttataattaa
     6961 attaataaat agcacggaga ggtataaatt atggtaaatg tatttgaaaa tcttaaacta
     7021 ttaaaactag acatgaaaga taaaggctgg gtgatagatt ccttttactt tcgttacaaa
     7081 cagcaaaatt atattgtact tgttaagcta tttgaaaaag aggaaaaagt tccagaatat
     7141 gctctgttaa aactagaatt tttaaaggaa aacgattttt cagatatgct agctgtctat
     7201 gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt tggtatcgaa
     7261 tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc tcgatttatt
     7321 ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta
     7381 agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag aagaaatcct
     7441 ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa aacgagattg
     7501 tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt cagatattca
     7561 atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac aaaaaataaa
     7621 attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt tttctatttt
     7681 tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt tggtcagtag
     7741 aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc tttacgcaaa
     7801 tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta ccaccacttt
     7861 ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc actgggttca
     7921 tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct atcattacct
     7981 atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa aaagaaacaa
     8041 tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc gattaaatat
     8101 atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta tgaattaatt
     8161 ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga taatttctgt
     8221 caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc aaccgaatca
     8281 agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt aaaagagata
     8341 gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg tgacaatcaa
     8401 gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga agtatccgta
     8461 aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac
     8521 aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt ttctaagatg
     8581 gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt ggagaaagat
     8641 gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt tgatgactac
     8701 gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa atacgatatt
     8761 ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat tgaacaggaa
     8821 gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga attggatttc
     8881 cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt tgataccagt
     8941 atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa
     9001 gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg
     9061 gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac caaagaatct
     9121 atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga gctgaaacaa
     9181 cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt acgacctttt
     9241 ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata tatcaatgac
     9301 tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc tacacaaatg
     9361 attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag aaatatttat
     9421 ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc attagcgtcc
     9481 gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct ggtctattat
     9541 tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg
     9601 aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac gagtgagaac
     9661 gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga ttcagaaagc
     9721 cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag cgagaagttt
     9781 cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg gttgttattg
     9841 gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc tgaccatatc
     9901 gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag tgtcacccaa
     9961 tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt cttacccgat
    10021 tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc aatgctgatt
    10081 gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt taaaattgtt
    10141 gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct atctatgcga
    10201 ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca aaacgcggac
    10261 gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt tcgttccact
    10321 gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga agatgaaaac
    10381 aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga catctatggt
    10441 cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc ctttgatacc
    10501 agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact
    10561 atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc acgctgggca
    10621 atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat gaatactcca
    10681 agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg ggctggcttc
    10741 catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat gccattacta
    10801 attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg gtgaaagaag
    10861 cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac atgcagacga
    10921 ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt ttcctccttc
    10981 tttttattct cattgttgga atctatgttg cctatacagg attggttaag cgagaaacga
    11041 cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg gcttccttta
    11101 ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg
    11161 ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa gggaaagaca
    11221 gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca tggctattgc
    11281 ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa agtttattgt
    11341 ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt gaagaaattg
    11401 aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta ggaacagtct
    11461 tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta acaggaatca
    11521 tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc agtttcctat
    11581 taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag ttatttaatg
    11641 tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt atttccacta
    11701 tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta caaattgtga
    11761 cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact ttacaaagca
    11821 acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac
    11881 aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca gggtcaacga
    11941 agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat actcgtcaaa
    12001 atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag caacaaggcg
    12061 gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa aatcgtctag
    12121 tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat gtcaaatatg
    12181 cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa caatctatgg
    12241 cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga
    12301 acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga aaagaaaacc
    12361 catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc
    12421 ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa
    12481 aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga acggataagc
    12541 gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct tctctctctt
    12601 cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca gtggcgaaaa
    12661 caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca
    12721 ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg tttcttatat
    12781 cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc aaagttccga
    12841 atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac aaggggttaa
    12901 atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag attctgttat
    12961 ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg ggaaaaaata
    13021 tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa aagcagacta
    13081 tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg gaaatcagtt
    13141 ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat ttgatgatga
    13201 aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct atgtctttgg
    13261 aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg tgtatgacaa
    13321 agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc aagaaatctc
    13381 tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata atgcaggcac
    13441 atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg caggcgaccc
    13501 aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg gggctagacg
    13561 tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa
    13621 agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta
    13681 aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt ggtagtgtcg
    13741 gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt gaaacagaaa
    13801 tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa tcctttgcga
    13861 aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat gaaaaattga
    13921 cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt aaggatattc
    13981 ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg aatgagaaca
    14041 cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac aaggaaacta
    14101 tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg gttattatca
    14161 aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa cagctagaaa
    14221 gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta gaaacgttct
    14281 tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt aatcatgtgt
    14341 tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc tttaccagaa
    14401 aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa
    14461 cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat
    14521 aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa
    14581 ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact atttaaaagg
    14641 agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa
    14701 ataattagat gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt
    14761 tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc tatggttatg
    14821 cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt
    14881 tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgca
    14941 ggaaaaacta ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga
    15001 agcgtggaca aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt
    15061 acaattcaga caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac
    15121 acgccaggac atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg
    15181 gcaattctac tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat
    15241 gcacttagga aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga
    15301 attgatttat caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc
    15361 aaacagaagg tagaactgta tcctaatatg tgtgtgacga actttaccga atctgaacaa
    15421 tgggatacgg taatagaggg aaacg
//
LOCUS       DAJSAW010000062        15230 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJSAW010000062 DAJSAW010000000
VERSION     DAJSAW010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.062"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            278..787
                     /locus_tag="ONT64_002242"
     CDS             278..787
                     /locus_tag="ONT64_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2303642.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ONT64_002243"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ONT64_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2303643.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ONT64_002244"
     CDS             2322..3302
                     /locus_tag="ONT64_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2303644.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ONT64_002245"
     CDS             3424..4842
                     /locus_tag="ONT64_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HCR2303645.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ONT64_002246"
     CDS             4913..6382
                     /locus_tag="ONT64_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2303646.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ONT64_002247"
     CDS             6392..6859
                     /locus_tag="ONT64_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2303647.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="ONT64_002248"
     CDS             6895..7767
                     /locus_tag="ONT64_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2303648.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="ONT64_002249"
     CDS             7848..10382
                     /locus_tag="ONT64_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2303649.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="ONT64_002250"
     CDS             10538..11311
                     /locus_tag="ONT64_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2303650.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="ONT64_002251"
     CDS             11304..12095
                     /locus_tag="ONT64_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2303651.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="ONT64_002252"
     CDS             12105..12596
                     /locus_tag="ONT64_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2303652.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="ONT64_002253"
     CDS             12614..13024
                     /locus_tag="ONT64_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2303653.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="ONT64_002254"
     CDS             13028..14428
                     /locus_tag="ONT64_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HCR2303654.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="ONT64_002255"
     CDS             14451..14825
                     /locus_tag="ONT64_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2303655.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5286 a         2135 c         3046 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag
//
LOCUS       DAJSAW010000063        14482 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJSAW010000063 DAJSAW010000000
VERSION     DAJSAW010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.063"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(287..649)
                     /locus_tag="ONT64_002256"
     CDS             complement(287..649)
                     /locus_tag="ONT64_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303656.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ONT64_002257"
     CDS             complement(723..1103)
                     /locus_tag="ONT64_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCR2303657.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1167..2360
                     /locus_tag="ONT64_002258"
     CDS             1167..2360
                     /locus_tag="ONT64_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR2303658.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2437..3021)
                     /locus_tag="ONT64_002259"
     CDS             complement(2437..3021)
                     /locus_tag="ONT64_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR2303659.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="ONT64_002260"
     CDS             3222..4220
                     /locus_tag="ONT64_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR2303660.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="ONT64_002261"
     CDS             4332..4841
                     /locus_tag="ONT64_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR2303661.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="ONT64_002262"
     CDS             complement(4871..5530)
                     /locus_tag="ONT64_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HCR2303662.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="ONT64_002263"
     CDS             complement(5532..6296)
                     /locus_tag="ONT64_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCR2303663.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="ONT64_002264"
     CDS             complement(6304..6981)
                     /locus_tag="ONT64_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCR2303664.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="ONT64_002265"
     CDS             complement(7018..8403)
                     /locus_tag="ONT64_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR2303665.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="ONT64_002266"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ONT64_002266"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="ONT64_002267"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="ONT64_002267"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR2303666.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="ONT64_002268"
     CDS             9342..10199
                     /locus_tag="ONT64_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2303667.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="ONT64_002269"
     CDS             complement(10280..10615)
                     /locus_tag="ONT64_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303668.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="ONT64_002270"
     CDS             complement(10682..12052)
                     /locus_tag="ONT64_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HCR2303669.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="ONT64_002271"
     CDS             complement(12130..12666)
                     /locus_tag="ONT64_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2303670.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12975..14456
                     /locus_tag="ONT64_002272"
     CDS             12975..14456
                     /locus_tag="ONT64_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2303671.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4461 a         2883 c         2353 g         4785 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 gctatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc
    12781 cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca
    12841 aggtaacaaa aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa
    12901 cacagactga aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata
    12961 aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg
    13021 tcattcaaaa aaataattgt tctttacaag aattatccaa tgacttacat attccaaaac
    13081 ggacaatcaa agaactgatc cgaaaaataa atgtatcact caagcaattt ttattgattg
    13141 atgaatttat ttattctaat cataaaggag agatccaaac caaagaaacc gatcaaacta
    13201 aaaagttagc tgttttttct aagttaaaat tatgctacct aaaagaatcc aatcgtttta
    13261 actatttgat gttactaatc aattatccac aaactgctgt accaaaaaaa tatttacttg
    13321 aacaacttta tatatcgccc tcctatctcg aaaaactgac aaaacagctc aatcattcct
    13381 taaaaaaatt ccatttacgg atcctatctg taaaaaattg ctgtactctt gaaggaaatg
    13441 agttatctat ccgtctctat ctttactttt ttctttcaga tacatttgga ggtaacgatt
    13501 ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa cagtacagat gagcaaatag
    13561 aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt aactttattc atattacgaa
    13621 acaaacaaca ggcatatttt ccaaaaactt cttctgaaat cactgaattg atggacttat
    13681 tgcagcatac acatgattat tcagaatact ttacttcttt ttcagtgagt cgcccttgtg
    13741 aagagacaga aacagagaga gcctacttta attatttttt ctattgtttt tttccaaatc
    13801 aaatctcttt tcgacaaaaa caggagattg gttttttatt ttccacaagc aaaaacctta
    13861 tttgtcgaca agtcggtcaa ttgatgactt acttaactca acagtttcct ctcaaccatt
    13921 tagaaaataa aataccaatt tatttttatt ttcttgtgtt ggcagttgtt tttttcgaac
    13981 tgttgggtga atccagcagt agtttttcta cactttgctt tccagaagcc acgtccaatc
    14041 attctagttc ttttatacag gaaaagaaat taagagaaaa aatcgataca attttttcaa
    14101 aaaatgggga aagaaaccaa atcagtcaat accttagtca attgatccaa cacttactac
    14161 aaacagaaac gaaagagcag ttactcatcc atttgcagat aaccaaacag tttacaggaa
    14221 attatcatct tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg
    14281 catattcaga acatgctgat ttgatcatta cagatcagca tgagaaaatt tcagatgaaa
    14341 cggagacttt ctatctggat acaacgcaca atacagtgtt atttggcaag ttaataaaag
    14401 cggttcatga actgtatatg aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa
    14461 attcttgtca catcctctta tt
//
LOCUS       DAJSAW010000064        14286 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJSAW010000064 DAJSAW010000000
VERSION     DAJSAW010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.064"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(221..1423)
                     /locus_tag="ONT64_002273"
     CDS             complement(221..1423)
                     /locus_tag="ONT64_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2303672.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="ONT64_002274"
     CDS             complement(1426..1620)
                     /locus_tag="ONT64_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCR2303673.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="ONT64_002275"
     CDS             complement(1667..2515)
                     /locus_tag="ONT64_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HCR2303674.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="ONT64_002276"
     CDS             complement(2720..3070)
                     /locus_tag="ONT64_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303675.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="ONT64_002277"
     CDS             3665..4111
                     /locus_tag="ONT64_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCR2303676.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="ONT64_002278"
     CDS             4735..5727
                     /locus_tag="ONT64_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2303677.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="ONT64_002279"
     CDS             5743..6627
                     /locus_tag="ONT64_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2303678.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="ONT64_002280"
     CDS             6676..8283
                     /locus_tag="ONT64_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2303679.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="ONT64_002281"
     CDS             8284..9768
                     /locus_tag="ONT64_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HCR2303680.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="ONT64_002282"
     CDS             9945..10937
                     /locus_tag="ONT64_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2303681.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="ONT64_002283"
     CDS             10989..11858
                     /locus_tag="ONT64_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2303682.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..13843)
                     /gene="guaA"
                     /locus_tag="ONT64_002284"
     CDS             complement(12278..13843)
                     /gene="guaA"
                     /locus_tag="ONT64_002284"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR2303683.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(13898..>14286)
                     /locus_tag="ONT64_002285"
     CDS             complement(13898..>14286)
                     /locus_tag="ONT64_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2303684.1"
                     /translation="RHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKR
                     GTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNNKIKLIK
                     RIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         4366 a         2424 c         2951 g         4545 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt tggctcttcg tcaaatcgtg ttggtgaaga caaatttgaa taaaacttag
    13861 tccgatatgc ggcgcgttct gtgcgccgca tattttttta gttttcaaag atcagacctt
    13921 gttgtaaata gatccgtgcc cgaaagttat aaaagttgcg ataaccaaac gcaattcgtt
    13981 taatcagctt gatcttgtta ttcagcccct cagtgacgcc gtttgagtgg gacaccttaa
    14041 aggcatttga aatacccgat tggtagcgat ggaagatcgt gaatttcttt ttaaacgact
    14101 gaggtaacgt agatgaaatt gttttaaggg tttcttgaaa gccttccgta cctcgtttaa
    14161 cgaaataata gcgtaataat tgacaaaaat gatacgcagt tcgcaattct tcgttgtagc
    14221 ttaacagctc atcaacgatt tctgtttgac taattggtcg tttaaacagt cgatgataat
    14281 gccgtt
//
LOCUS       DAJSAW010000065        14107 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJSAW010000065 DAJSAW010000000
VERSION     DAJSAW010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.065"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            66..767
                     /locus_tag="ONT64_002286"
     CDS             66..767
                     /locus_tag="ONT64_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCR2303685.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="ONT64_002287"
     CDS             764..1885
                     /locus_tag="ONT64_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCR2303686.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="ONT64_002288"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="ONT64_002288"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR2303687.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="ONT64_002289"
     CDS             3264..4172
                     /locus_tag="ONT64_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HCR2303688.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="ONT64_002290"
     CDS             4207..4479
                     /locus_tag="ONT64_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HCR2303689.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="ONT64_002291"
     CDS             4490..5536
                     /locus_tag="ONT64_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HCR2303690.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="ONT64_002292"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="ONT64_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR2303691.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9233)
                     /locus_tag="ONT64_002293"
     CDS             complement(8547..9233)
                     /locus_tag="ONT64_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303692.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(9211..9705)
                     /locus_tag="ONT64_002294"
     CDS             complement(9211..9705)
                     /locus_tag="ONT64_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCR2303693.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="ONT64_002295"
     CDS             complement(9725..10945)
                     /locus_tag="ONT64_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2303694.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="ONT64_002296"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="ONT64_002296"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR2303695.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(13021..>14107)
                     /locus_tag="ONT64_002297"
     CDS             complement(13021..>14107)
                     /locus_tag="ONT64_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2303696.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         4555 a         2592 c         2919 g         4041 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatctt ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaaccta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc caatagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggacgagg acgaaccgaa gaaatagcaa gggtttgttt gaaaacagtg taaaaaataa
    12961 accatgttta catggatttg ccaccggttt gccaccggtg gtttttttcg gtggcaaaaa
    13021 tcaagaattc tttctaagat tttccatgat atcgaccgtt tcatttctca tcttatctgt
    13081 tacatgcgag taagtatcca tcgtaattga aattctgctg tggcccaatc gttcagagat
    13141 ttctttcatt tttgcgccat tttcgagaag aagtgtagca tgagtatgtc tgagagaatg
    13201 gaagttaaaa gagagggaga gtgcattcga tatcctccta gtattccatt tcactacact
    13261 tggcgtaact agctcaccgt cttccttcgt acatactgca tttgaatcaa tgtagagctt
    13321 tccatacttc attcgatttt ctaattgttg tttcttatgt tttttcagaa ttgctagcaa
    13381 ggtttgtcca ataaaaatcg ttcgattgga actgcttgtc tttggcgtac catataccca
    13441 tgcgccgtca ttctttacca tttgtttctc tacagtaatt gttccatttg aaaaatcgac
    13501 atcatcccac gtcagaccac aaacttcgcc aacgcgcatt cccgtataaa atccaatatt
    13561 caaaggaata tagaaaggat gtccttcagg agtaatttct agcatatgat caaagtcctc
    13621 aagagaaatg atttttagat cttttttagt cgttggtcgt gcttcatatt ttggtatttt
    13681 tacatacagc ataggatttt gcttgattaa tccccaagga taaaccgcca tattcagcgc
    13741 attcttaagg acagagtgag taatagtcat tgttttcttc gagtaaccct ttttaaattc
    13801 agcattgatg aaattttgta aaagagcagg ggagagatcc gtaagttttt tctttcctaa
    13861 ataaccgttt atatgatttt tgatggtaaa tcggtagttt tcataggtat tgtattttag
    13921 atttagttta acgtattcct ccatccaaaa atcaaggtat tgttttactc gagtatccgt
    13981 acctaaaaag tattgtcctg tttcgtcaat atctgataaa acttttcgta aagcagcttc
    14041 ggcctctggt cgggtgtctc cgccaacttt ctccactttt tttcttgagc catcatcatt
    14101 gatatct
//
LOCUS       DAJSAW010000066        13852 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJSAW010000066 DAJSAW010000000
VERSION     DAJSAW010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.066"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..902)
                     /locus_tag="ONT64_002298"
     CDS             complement(<1..902)
                     /locus_tag="ONT64_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCR2303697.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="ONT64_002299"
     CDS             1185..2153
                     /locus_tag="ONT64_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2303698.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="ONT64_002300"
     CDS             2266..2544
                     /locus_tag="ONT64_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR2303699.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="ONT64_002301"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="ONT64_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR2303700.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3001..3756
                     /locus_tag="ONT64_002302"
     CDS             3001..3756
                     /locus_tag="ONT64_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR2303701.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="ONT64_002303"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="ONT64_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR2303702.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="ONT64_002304"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="ONT64_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR2303703.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="ONT64_002305"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="ONT64_002305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR2303704.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="ONT64_002306"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="ONT64_002306"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HCR2303705.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="ONT64_002307"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="ONT64_002307"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR2303706.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="ONT64_002308"
     CDS             8493..9035
                     /locus_tag="ONT64_002308"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR2303707.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /locus_tag="ONT64_002309"
     CDS             9058..10614
                     /locus_tag="ONT64_002309"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR2303708.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="ONT64_002310"
     CDS             10628..11530
                     /locus_tag="ONT64_002310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR2303709.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="ONT64_002311"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="ONT64_002311"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR2303710.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="ONT64_002312"
     CDS             12973..13395
                     /locus_tag="ONT64_002312"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR2303711.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4575 a         2474 c         3013 g         3790 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatttggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca
     9901 ggtcgtgaag cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgtctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaaata attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgttgctc agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg
    13561 gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg
    13681 gaataaggga ctgcgacaag acttttgtgg ctctttgtca ataaggactg atgagttgtg
    13741 caaaatcaaa tctgagtcag aatgaaccac attctggctc agattttttg ttatcttaca
    13801 ttgattaatt gattgatcaa aaagattcta attctcatgt tctcaaatga tt
//
LOCUS       DAJSAW010000067        13429 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJSAW010000067 DAJSAW010000000
VERSION     DAJSAW010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.067"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..914)
                     /locus_tag="ONT64_002313"
     CDS             complement(<1..914)
                     /locus_tag="ONT64_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCR2303712.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ONT64_002314"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ONT64_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR2303713.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="ONT64_002315"
     CDS             2006..2176
                     /locus_tag="ONT64_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303714.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="ONT64_002316"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="ONT64_002316"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR2303715.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="ONT64_002317"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="ONT64_002317"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR2303716.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="ONT64_002318"
     CDS             4440..5321
                     /locus_tag="ONT64_002318"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR2303717.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="ONT64_002319"
     CDS             5318..6034
                     /locus_tag="ONT64_002319"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR2303718.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="ONT64_002320"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="ONT64_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCR2303719.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="ONT64_002321"
     CDS             6751..7752
                     /locus_tag="ONT64_002321"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR2303720.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="ONT64_002322"
     CDS             7749..9110
                     /locus_tag="ONT64_002322"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCR2303721.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="ONT64_002323"
     CDS             9070..10221
                     /locus_tag="ONT64_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCR2303722.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="ONT64_002324"
     CDS             10196..11365
                     /locus_tag="ONT64_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR2303723.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="ONT64_002325"
     CDS             11540..12919
                     /locus_tag="ONT64_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR2303724.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="ONT64_002326"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="ONT64_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DAJSAW010000068        13398 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJSAW010000068 DAJSAW010000000
VERSION     DAJSAW010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.068"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(14..706)
                     /locus_tag="ONT64_002327"
     CDS             complement(14..706)
                     /locus_tag="ONT64_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR2303725.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="ONT64_002328"
     CDS             complement(1143..1712)
                     /locus_tag="ONT64_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR2303726.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="ONT64_002329"
     CDS             complement(1786..2109)
                     /locus_tag="ONT64_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303727.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="ONT64_002330"
     CDS             complement(2263..3075)
                     /locus_tag="ONT64_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303728.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="ONT64_002331"
     CDS             3429..4277
                     /locus_tag="ONT64_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR2303729.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="ONT64_002332"
     CDS             4422..5363
                     /locus_tag="ONT64_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HCR2303730.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="ONT64_002333"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="ONT64_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="ONT64_002334"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="ONT64_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HCR2303731.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="ONT64_002335"
     CDS             complement(9827..12013)
                     /locus_tag="ONT64_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCR2303732.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="ONT64_002336"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="ONT64_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCR2303733.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="ONT64_002337"
     CDS             complement(12235..12675)
                     /locus_tag="ONT64_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HCR2303734.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="ONT64_002338"
     CDS             complement(12749..13288)
                     /locus_tag="ONT64_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR2303735.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2380 g         4446 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaataa
//
LOCUS       DAJSAW010000069        13348 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJSAW010000069 DAJSAW010000000
VERSION     DAJSAW010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.069"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            237..1976
                     /gene="yfcC"
                     /locus_tag="ONT64_002339"
     CDS             237..1976
                     /gene="yfcC"
                     /locus_tag="ONT64_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR2303736.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2133..3317
                     /gene="iadA"
                     /locus_tag="ONT64_002340"
     CDS             2133..3317
                     /gene="iadA"
                     /locus_tag="ONT64_002340"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR2303737.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3428..3631
                     /locus_tag="ONT64_002341"
     CDS             3428..3631
                     /locus_tag="ONT64_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR2303738.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3732..4394)
                     /locus_tag="ONT64_002342"
     CDS             complement(3732..4394)
                     /locus_tag="ONT64_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2303739.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4407..5909)
                     /locus_tag="ONT64_002343"
     CDS             complement(4407..5909)
                     /locus_tag="ONT64_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2303740.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6457..8136
                     /locus_tag="ONT64_002344"
     CDS             6457..8136
                     /locus_tag="ONT64_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCR2303741.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8263..8742
                     /locus_tag="ONT64_002345"
     CDS             8263..8742
                     /locus_tag="ONT64_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HCR2303742.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8961..9641
                     /locus_tag="ONT64_002346"
     CDS             8961..9641
                     /locus_tag="ONT64_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR2303743.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9649..10980
                     /locus_tag="ONT64_002347"
     CDS             9649..10980
                     /locus_tag="ONT64_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR2303744.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10995..11957
                     /locus_tag="ONT64_002348"
     CDS             10995..11957
                     /locus_tag="ONT64_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HCR2303745.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12068..12406
                     /locus_tag="ONT64_002349"
     CDS             12068..12406
                     /locus_tag="ONT64_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HCR2303746.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12485..13192
                     /locus_tag="ONT64_002350"
     CDS             12485..13192
                     /locus_tag="ONT64_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCR2303747.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4274 a         2343 c         2917 g         3814 t
ORIGIN      
        1 gattttctct aatctaaaaa taaaaaaaga attttaagga aaaacagaga aataaaatac
       61 ttaatataaa tggaaagaga aaaaaataat catacatagg agaaaaaaaa caaaatagaa
      121 aaaagtgatt ttctaataaa ttattcatgt ggaaatgtca tttttactgt ataattcatt
      181 tgattatcca ttttttattc atcaaaatta ctgaaaaaag aaaggaagaa ataaatttga
      241 agaaaacaaa ttcttcagga aaaccgaaac gcgacttcac taaattatcg acaccccata
      301 cgtatgtcat tatttttggt gtagtcatct ttgcatggat actgactttt gtcgttccag
      361 caggaaagtt cagcacgcaa gatattgaat acaaagatgc aaatggcgaa acaagtactc
      421 gtactgtttt acgtcaagat tccttccgtt atgcttacga gctggataag tcgtatgtgt
      481 ttgatcaatt agaagaactg caagatcatc cagcagaacg tgaaaaactg gatgttccgg
      541 aaaaaggatt ggaaaaagtc attgctgacg gagaaaaaaa tcttacgcag gaaaaactag
      601 atgaaatctc cttgacagat gatgtgcttt acgatcagta cggcgaaaat atctatgata
      661 cttctaaaaa attgcataaa acagccaaaa tatggggaac agatgatttt ggcggctttg
      721 gtttcttaaa ttttgttttt gaaggactag tatctggaga taagtatgga tctgctgtag
      781 ggatcgctgc attgatcctt gttgttggag gtgcttttgg tattattatg cgtaccggag
      841 cgatcgacgc aggtatctat gcgtttatca gtaaaactaa gggactggag cgtttagctt
      901 taccgctatt attctttgct ttttcattcg gtggcgctac attcgggatg gcagaagagg
      961 taattccatt ctcaatggtg atggttccat ttgtcattgc gttaggatat gactcgatcg
     1021 tagcagtaac cgtgacttat gtcgcatctc aagtcggaaa tgcgacctct tggatgagcc
     1081 cgtttagcgt agcggtggct caagggattg ctggaatccc cgtgttgtct ggtgcgacat
     1141 tccgattgat tatgtgggta gttgttactg cattagccgc tggttatatg atgatatatg
     1201 cagaaaaaat ccgtaaaaaa ccagaaagtt cgttgactta caaatcagat gattatttcc
     1261 gttctcatat caaaaagaca tctgatgaaa ataaaaaatt catgcttggg cataaattga
     1321 tcttattaga gatgttagtt gtccttgtct ggatcgtttg gggcgtgact caaaaaggct
     1381 attacattcc agaaatcgct tctcaattct ttgtgatggg attagttgct ggaatcattg
     1441 ctgttctttt caaattagat ggaatgaaag tcaatgacat cgcttcttct ttccaatctg
     1501 gtgcagctga tttagctggt acagcaatcg tagtaggtat ggccaaaggg attcttcttg
     1561 tcttgggagg atctgatgca tctgttcctt cagctttgaa tacgattttg catgggatcg
     1621 ggacggcgtt gaccggtgtt ccagctgtca ttggtgcttg ggcgatgtac atcttccaaa
     1681 gtttgttcaa cttggttgtt acatcaaatt caggacaagc agctttgact atgccaatca
     1741 tggctccact ggcagattta gtaggtgtat ccagacaagt agcagttctt gcttatcagc
     1801 ttggttcagg ttttatggac gcttttacac ccgtatcagc cagcttgatt ggtgtgctag
     1861 gtgttgcacg gatcgaatgg gcaaaatggg caagattcca aatcaagatg caaggattcc
     1921 tgtttgtttt aggaactatt ttcatcatga ttgccattgc aataggtctg cagtagtact
     1981 ggagacctgt aaataaaaaa gtaatccaaa cagtagataa ggactccaag aaccgtcatt
     2041 ttacggtaaa atcaaaggag taaagatcct atcgtttgga ttatttttta agatcctatc
     2101 atcagttata cttaaagaaa gaggaaaaat gtatgaaaat cattcgacaa attgaagtgt
     2161 ttgcaccaga ttacttagga aaaatggatg tactgattgc cggtgggaag attttggctg
     2221 tagaagaaca gctagaaggc ggctacgaag gagtggaagt agaagaactt tcaggtgagg
     2281 gaaaagtact aaccccgggt ttcattgact gtcatttcca tattttaggt ggaggaggag
     2341 aaggcggtta tcaaaatcga acgccagaag tcacattgag ccaattgact acagctggtg
     2401 taacgacagt tgtcggttgt ttgggaacag acggagaagg tagagatatg actgcgttga
     2461 tcagcaaagc gaaaggtctg gaagcagaag gaatctccac ttatgtatac gaaggttcgt
     2521 accgattgcc ggtaaaaaca gtaacagatt ctatcatcaa agactttttg acaattgata
     2581 aaattatcgg aatcggagaa atcgcagtat ccgatcaccg ttcttctcag ccaagttttg
     2641 aagagttcgc tcgtgcagta gcagatgctc gtgtaggagg catgttatcc ggaaaagctg
     2701 ggatcatcaa tgtccactta ggcggaggaa aaagaaagct agagctattg acacgaatcg
     2761 tagaagaaac agaaattccg atcacacaat ttttgcctac tcatgcaaat cgcacaccag
     2821 aattatttga agcttgcgtg gcatttgcca aacgtggtgg cacgattgac ttcactgcta
     2881 gcgaagatcc tgatttttgg gagaagacag atggtgaagt tcgcttcagc aaagcactga
     2941 aacgattgat cgaggaagat gtttctttag ataatttcac gatgacctca gatggacaag
     3001 gaagcttgcc ttattttgat gaaaacaatc atttcctagg gttaggagta ggaagtgcca
     3061 aggcattgct agtagggatc aaagaagctg tgcaaaaaga aagtatccct ttggaaatcg
     3121 ccttacgagc tataaccagt aatccagcac gtatattgaa attggataaa aaaggaaaaa
     3181 tcgaaattgg tgctgatgca gatctgtgta tcttggataa agaaacgctt gatattgata
     3241 cagttatcgc taaaggagaa ataatggtcc aagaaaaaga agtcaaagta tgggggactt
     3301 tcgaaaaaag tttttaatca acagctctaa ctatcttttt tagaaaacga gcgttctgtt
     3361 tgtctgtatc tgttgttgat gttaccctga aggtagatca ataaacaaac taagaggagg
     3421 aagcacaatg gcagatttaa aaggacgttc taaagatatg aaagataaag tagtcggtga
     3481 aggaaaagaa gcatacggca aagcaacaga cgacaagagc aaagaaacag aagggaaagc
     3541 acaatcacta ggtgctgaca tcaaagaaaa agctcgaaat ttaggcgacg aagtcaaaga
     3601 caagttttct cgaaaagacg aaaaaaaata aaaaccactt attactaatt gaatcaagca
     3661 aataaaaaat ccgaaccata tctgcgagac agatatggtt cggatttttt atttgatcat
     3721 tatcctgttt tttaaacttc gattttttga aattttttat tttttagttc aaagacttca
     3781 tcacagcttt ttgctacttc aatctcatga gtcacgatga taatacagcg attttgttca
     3841 tgggcgattt cttggaaaag tttgacgata tcttgtgaag ttgtttcatc tagatttcca
     3901 gtaggctcat ccgctacgat tagttgatgt tcacagcaga tagcacgaac gatagcgact
     3961 cgctgctgct gacctcccga aagtttggtc acctgttttt ttgctaatat ttcatcaatt
     4021 cctacacgag ccagtcgatc taaagcaact tgtcgataat tctcttgctt ggtcttagtg
     4081 atttccattg ctgctatgac gttgtctaga gccgataaat aaggaagcaa gttgtaagct
     4141 tggaaaataa tcgagacatc atttttacga taacttctta acccgatttt gttcagtgaa
     4201 cgatcttgat agtatacctt tccttctttt ggtgcatcta gtccagaaat aagcgaaaga
     4261 aaagtcgttt ttccagaacc acttgctcct aaaatagcgt acatttttcc agcttcgaaa
     4321 ttcagttcta catctttata aagcgcatct tgcggatctt gataccaaaa tcccaattgt
     4381 tctgtccgta acatcaaatt cctcctttag tttaatagga tttttcttgg gtttaatctt
     4441 aatatcccta tagaagctaa aaagacagaa aacagactga ttccaaatgc tactccggct
     4501 aatagtccca attgtgcagg tttgacattg atttccaatt cgctgacttg atcagaaaca
     4561 gcaaatggat tattcgtggt tccttgttga ccatcgcgtt gaccgccttg ttcttgtctg
     4621 ttacctggca tttgtccacc gccaggagct tgctggtttt gtgtcgtggt ttctgtctgt
     4681 tggtcaagta gctgctgtcc cacggcattc gcgacgacgt ttccgctaag cgaagcaact
     4741 cctaatgcaa gaatcataca aatagccact tcggtaaaca attggagaat gattttactt
     4801 ctggattctc ctaaagatag caagacgcct aactcatgcc gacgctcccg aatggtaatc
     4861 ataatgatca gagtaagtat gataataccg gctacagcca ctagtaaaac aacattttta
     4921 gaaaaacttg ccacgttgtt taaaggttcc aacattgact gatacatgga atcatttgat
     4981 tgtaagctaa atgtatctgt gtcgatcagc ttctcagctt ctttgagaaa ctcatccatt
     5041 ttatctggat cagatagagt gtaaatagca gaatctatcg tgttatcgtc agatgtccca
     5101 tttagttcat ttgcaaaagt gtaagaagtg taaagtgtat tcgacggatt catgaagttg
     5161 aatttcatac ccatactgcc gcttgtttcg ctggattcat aaattccttt gatcgtcatt
     5221 tcataagtgg tatcttcatc ttcagtactc gtgattgtga aagtatctcc gactgccagg
     5281 tcatttgttt ctgctagagt agaatcaatg actacattgt ttgtgccttt gtcgctgtca
     5341 tctattcctt caccatccgt gatttttgct gttccatcag aaaaattact attttgtgca
     5401 gttgctgaca caccactgat ttgaaagtct gcttgagtca tttgcggatt cccgcccatt
     5461 cctccagcag gttcaccgcc tgcatcagta gtttctgaac tgtcttcact tgaatcagaa
     5521 ctagtgatgg cagttattcc atcttttgcc atagctgagg cggaactttc aaaggaataa
     5581 ctctttacat tatctaattc agctattttt tccgcgtccg ttaaggaaac tggtgtaaga
     5641 gaaaaactgc ctggatctgg acgttcttca gtggtagaac cgcttgaaga agtttgttcc
     5701 tgttttttaa atgaagcttc tcgattggca cttaaagtca ctgttgctcc aacttctttt
     5761 tgtgcttgat tcgctgcttc ttctgcagcg cttcgaatcg tgaggccagc tagcacaaaa
     5821 ataagaatgg ctgtgaacac ggcaaacaat aacaatgaac gtccccattt tacttttgta
     5881 cttttccatg cacgtttcat aaaattcata ttttgcatcc ctcctagtag atgcatttga
     5941 taatagacaa cctatcttaa agctagctta aatcttatgc tgtttgcaca ccatcaacat
     6001 gaggttctgt atatataaaa agtcactata gttattgcaa aaaatgtggt aaaatgaaaa
     6061 aagtatgttt caaaccgacg aagaagaaat gaaagaggaa tagcaaaaga aatcctagat
     6121 gctacagagg gatttttacg tgcatagttg ttctaacaat tgctgatact agcgatttag
     6181 tttatttgtt gtataataat ggggaacagc tgaatactta tataagtatt ctggctatag
     6241 cgtttcgcaa aagatccatt ttgttaatga agtatatcca aaataaaatg cccatccaac
     6301 tgtcagaacg cggatgtgct ggtaattttc ctgctgattc tccataagtt gagaaatagt
     6361 gattttgcat atgcttcatt ggaatggctc atatatttag ataaagaaga aaatcgacat
     6421 ctttaaactt ggatgtcata agaagaggtg aagtttgtga gtaccattaa aattatccca
     6481 ttaggcggcg ttcgtgaaaa tggaaaaaac atgtatgtcg cagaagtggg cgaggatatt
     6541 tttattttag attgtgggct aaaatatcct gaaaatgagt tgttggggat cgacgtcgta
     6601 atccctgatt ttacgtattt agaagaaaac attgatcgtg tagctggtgt ctttttaaca
     6661 catggacatg cagatgcgat cggggcactt ccttacttgt tgtcaaaaat ttctgtacca
     6721 gtattcggga cagagttgac gatcgagtta gcaaaattga atgtcagtcg taatgaagca
     6781 gccaaaggat ttaaggattt ccatgtggta gatgagcata cagaaataga ctttggtgaa
     6841 acagtagtga gctttttccg taccactcat acgataccag attcagtggg gatcaatctg
     6901 aaaacaaaag aaggtagtat cgtttatacg ggagatttta aatttgacca aacggctatc
     6961 ccaatgtatc aaacagatta tgcacgattg gcggaaatcg gcaaagaagg agtcttagcc
     7021 ttactaagtg aatcggcaaa tgcagaaaat cctcagcctg tagcttctga agcccaaata
     7081 gcagatgaag tatttgatac gatcaaatat tgggaagggc gtatcatcgt cgcttgtgta
     7141 gcaagtaatt tgcaacgagt gcaacaagta ttggatgcag cagaaaaagc acaacgaaaa
     7201 gttgtcttga ctggtcaaga tttcgaacga attattcgca cagcgatgcg tttagaaaaa
     7261 cttcaattac caagtgaaga tctgcttgtt acgttgaaag acatgaaaaa ctatgatcca
     7321 gaagaactga tcattttaga aactggccga atgggtgaac ccatcaagtc tctgcaaaaa
     7381 atggcaaacg gtacacaccg taatgtcaag attgaagaag gagatttggt ttatatcact
     7441 actacgccaa gcatcgcgat ggaaaccatc gtggcgaaaa cggaagatat catttatcga
     7501 gctggtggaa cagtgaagtt gatctctgag aatatgcgcg tttctggcca tgccaatcct
     7561 aatgacttgc aattaatgat caatttgatg aaaccaacat attttgtgcc agttcaagga
     7621 gagtatcgtg aattagctgc tcatgcagat ttggctcatg cagtagggat gccatacaaa
     7681 aatatctata ttactggacg cggagacgtg ctggaatata agaacaagcg tatgagtgtt
     7741 gcaggaacta caagtgcaga aaatgtaatg attgatggat taggcatagg tgatatcgga
     7801 aacattgttt tacgtgatcg aaaaatcttg tcagaagacg ggattttcgt agcggttgtc
     7861 actatcagtc ggcgagaaaa gaaaatcatt tcaaaacctc aaattacttc acggggattc
     7921 gtttacgtaa aagctagccg tgatctgatg aaagaaagca gcaatatgat tgaaacaatc
     7981 gttgagaaac atttagaaag caatgatttt gagtggagca aattaaagca agacatccga
     8041 gatcaattag gccgttactt atttgaacaa acaaaacgcc gcccagtgat cttgccagtc
     8101 attatggaag ctactcaacg tcgaaataaa aaataaacaa aaagagtaag atgaacctct
     8161 ctatagagtg ctcgtcttgc tctttttttg cgaaaaacaa ataatttttg aatacagcat
     8221 ccctcgcttt ataataaacg atagaataaa gggagtgaat caatgatgag tcaaacaaaa
     8281 gtcgaacgtt atttagaaga acaaaagatc agctatcaac cacttgattt tagtcatgtg
     8341 gtagaagaat ctttcacaga agaactacaa aaaagaggga ttgatccagc tttgatatgt
     8401 aaaactctcg tagtgaaagg aaacaaaact ggggtgatca tcgcattgat tcctttgaac
     8461 gatcacttag attacaaaaa aacgagacaa gtgacgaaag atcgtaaagt tggttttcct
     8521 ggaatggatt ttgtattaat gcatacagga tatccccacg gagcaaacac ccctattgga
     8581 ataaaactcg cacatcctga ttattgtttt ttagcagaca gttctttaaa agaaaaagaa
     8641 gaagtaattg tgtcaagcgg tgaaatcggg agaagccttc ggataaaagt agccgattta
     8701 gaaaagatcg tccatcctat ttacgctgat ttaattagtt aagggacgag aagagaagct
     8761 aagcagagca gagacctcaa acttgagatt atatctgctt ggtacttggt gcatcctgta
     8821 ttatctgtta caattgcttt gtacatactg tactatcaat ccaatgaaaa tatatttagc
     8881 agctatattt tttcattgat gtgcaagtga tgaaatcgtt tttaataaaa aagatacaga
     8941 gaaaacgcga ggtgtaaaat atgaaaatta cgtatcatgg acattcctgt ttaacaattg
     9001 aaatggaaaa tggccaacgc gtgatgattg acccgtttat tacaggaaat ccattgacag
     9061 acttaactgc agaagaagta aagacagatt ggttgctgat tactcatggt catagtgacc
     9121 acataggaga tatgattcct attgcaaaag aaaatcaagc cacaattatc agtattgttg
     9181 agattgctga ttatgcgaac agcagaggag tagattcatt cggtatgaat atcggtggaa
     9241 aacatgcgtt tccattcggc gtggtaaaat tcgtacatgc acagcatagt tcaagctatg
     9301 aagtagacgg tatcgttcag tatatgggcg agcctagcgg aattattatt caagcagaag
     9361 gaaaaacgat ttatcatgca ggggatactg cttatttcag tgatctagga ttgcttgcag
     9421 aagaattcga tattgatgtc gctttcttgc cgattggcga taactatacg atggggccag
     9481 aagatgcagc aaaggctgcg caaacaattc gtgccaagaa agtagttcca atccattaca
     9541 atactttccc tgtcattgaa caagatcccg aatcttttgt agagttactg gaagatagtg
     9601 aaggaaaaat aatgagcgtc tcggaaacgc tggaagtgta ggcagaaaat ggcgacaaaa
     9661 catgatttga ttttagaata tattgaaagt ttacctgttg gcaatcgaat ttctgtaaga
     9721 agcatcgcaa aagaattgaa tgtcagtgaa ggaacagcgt accgtgcaat caaagatgca
     9781 gaaaatgtcg gactagtttc aacaattcaa cgagtaggga ccatccgtat cgaacgcaaa
     9841 ttaaaaaaac atattgaacg tttgacattt ggcgaggtcg tcaggatcat tgaaggggac
     9901 gttttaggcg gggcggcagg attagacaag gtcttgaata aattcgtcat cggcgcgatg
     9961 actaaacatg ccatggaacg ctatattacg ccaggatcac taatgatcgt cggcaaccgg
    10021 gcggaagtcc aaaaattagc attggaagat ggcgccgcag tattgatcac aggaggattc
    10081 aatacgactg ctgagattgc aaagctagct gatgatcttg aaatgccgat cttacgcacc
    10141 tcttatgata catttacagt cgcaaccatg atcaaccgag cgttaagcga ccaattgatc
    10201 aaaaaagata tcatgcttgt cagtgatatc tatatgccag ctgaaaagac tcattatttg
    10261 cagcagacag atacgatttc tgattatcag cgtttgtcag aagaaacgca gcattcgcgt
    10321 tttccagtcg tcaaccgcca tcatcgtttg atggggattg ttacggcaaa agatgtttta
    10381 gggaaatcgc caaaccagat catcgatcgg gtgatgacga aagaaccaat cagtgtaaag
    10441 aaaacaatga gtattgcctc tgttagccat caaatgatct gggatgggct agaagtcatg
    10501 ccggtcgttt ccgatgactt gacattggaa ggcttgatta cccggcaaga tgtaatgaaa
    10561 gcgatgcagc ttgtccagcg acaaccgcag attgcagata cgatttcgga tcagatatct
    10621 ggaaatatcc atacgattga tactgaccgt gaaggaaatc gtttggaaaa ccctaagttc
    10681 aaattcgtag tagcaccaca aatggtcaat ggcgttggaa ccatttcttt tggcgtactt
    10741 agcgaaatca tttctgatgt cgcacaaaaa acaatggtga tgaaccaaaa acgaaatatc
    10801 ttgattgaac aagtgaacct acattatttg cggctgatcc aattggaaag cgaattagac
    10861 attcgtccac gtgtattgga aatcggcaga cgttccgcta aactggatat cgaagtgttt
    10921 attgaaaaca ccatcgtttc caaagcaatc gttgtttgtc aagtaatgga acgttcttag
    10981 aaagcaggag tgaaatgaca gtacagcaag aaatagtaga tacaataaaa aaatacgata
    11041 aaatcattat ccacaggcat ttgcgccctg atcctgatgc attaggatca caagtaggat
    11101 tagccgaact attacgggaa agtttcccag aaaaagaaat acgtcaagta ggtgaaacga
    11161 ttgaagggct tcgcttttta gcagaaatgc aagaagttga agatgtcttc taccaaggag
    11221 cgctagtcat cgtgactgat acagctaatt cacccagaat cagtgatcaa agatatcagt
    11281 tggcagaaaa actgatcaaa atcgaccatc atcccaatga tgacccttat ggagatcttg
    11341 tgtgggtaaa tacaaaagca agtagctgta gtgagatgat tgcagaattt gctttgatgt
    11401 ttcctgaatc tttaaagatg aacgttaagg ctgcacggtt actttatgca ggaatcgtcg
    11461 gagatacagg gcgctttttg tatccctcca cgacgtcccg tacattagag attgcttcga
    11521 tattgagaaa ttatccattt gatgcaagtg ctttgaacag agagattgaa caaatgccga
    11581 tgaaagtcgc taaactttcc ggttatcttt atcaaaatat ccaggtggat gaaaatggtg
    11641 caggaagaat aatccttccg caagaaattc ttgataaata tggaatcgat gattcagaga
    11701 cagcttcgat cgtttcactt cctggagtga ttgacgaagt gctggcttgg gggatttttg
    11761 ttcagcagcc ttcagggtat taccgcgtac gtttacgttc taaggggccg atcatcaatg
    11821 aattggctaa acgccatcat ggaggaggcc atccattagc aagtggtgcg aatgcaaaag
    11881 atttagcaga agtagaggaa atctataaag agatccaaga tatatgccaa tcgtataaga
    11941 gcagaaaaac agaatgatac ttcgttcgaa aacgtgcctt ttgtagtaaa aatcacaaat
    12001 gtatcttttc aagatcttct ttttccgtta caatagtgct cactaaagat tttaaaggag
    12061 aaagaagatg acattaccaa attgcccaga atgtggctca gagtacacat atgaagatcg
    12121 aggcttgttg atttgcccag aatgcgggaa cgaatggaac aaagaagaac aagaaactgt
    12181 catagaagac ggattagtgg tattagatgc aaatggaaac cgtctgttag agggcgatag
    12241 cgtgacagtg attaaagact tgaaagtaaa aggtgcgtca ggtgccatta aacaaggaac
    12301 aaaagtcaag aacatccgtt tagtagaagg cgatcataat atcgattgca aggtagaagg
    12361 atttggaccg atgaaactga aatcggaatt tgtgaaaaag aattaaagaa tattcaaggt
    12421 cttttcacat tcttttccta ctactaatga taaaataaag taaacgaaaa ggagtgaaac
    12481 gaaaatgtat ggttatggaa tgtatggttt tttagatccg acatttcttt tggtcattct
    12541 tggattagtt atttccggaa ttgcgtcagc gtacgtcaat agtactttcc gaaaatacga
    12601 tcaagtgaga agcaaaaaca atgtgactgg tacacaagca gcacaatata tattacaaag
    12661 tcaacagata aataatgtcg gtgttcaaca aattgccgga gatctcacag ataactacaa
    12721 ttcaggtaat aagatgttga gtttgtctca agcaactgca cagtctactt cagttgctgc
    12781 gataggtgta gcagctcatg aatgcggaca tgcggtacaa gatgcttcgg gttatgtccc
    12841 attgaagatt cgtgcagcac ttgttccagc agctaacttt ggttcgatga tttcgttccc
    12901 cttgattatg gtaggtgtat tgcttagctg gaactctacg ttaatcaata tcgggatttt
    12961 cgctttttct ttagcattac ttttccaatt ggtgacatta ccagtcgaat ttaacgcgtc
    13021 tcggcgagca atccaaattt tgagtgaagg cggactactg acggaagaag aagtaccgat
    13081 ggcaagacac gtattatttg cagcagcact gacttatgtt gcagcagcgc tttcgacatt
    13141 cctgcagctg ttgcgcttga tcttattgtt cggcggcaac cgaagagatt aaaataaaga
    13201 agttgtgaaa tcagcgcttt gatctgagct aataccgaga gttagctgat tgaataccat
    13261 ttatttggaa taagggctct ttgtcaataa ggactgatgg gttgtacaaa atcaaatctg
    13321 ggtcagaatg aacctcattc tggctcag
//
LOCUS       DAJSAW010000070        13284 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJSAW010000070 DAJSAW010000000
VERSION     DAJSAW010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.070"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..276
                     /locus_tag="ONT64_002351"
     CDS             <1..276
                     /locus_tag="ONT64_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303748.1"
                     /translation="SELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPVRTRSAKG
                     IYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(373..807)
                     /locus_tag="ONT64_002352"
     CDS             complement(373..807)
                     /locus_tag="ONT64_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2303749.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1039..1749)
                     /locus_tag="ONT64_002353"
     CDS             complement(1039..1749)
                     /locus_tag="ONT64_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR2303750.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1739..2599)
                     /locus_tag="ONT64_002354"
     CDS             complement(1739..2599)
                     /locus_tag="ONT64_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HCR2303751.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2604..3242)
                     /locus_tag="ONT64_002355"
     CDS             complement(2604..3242)
                     /locus_tag="ONT64_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR2303752.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3257..3556)
                     /locus_tag="ONT64_002356"
     CDS             complement(3257..3556)
                     /locus_tag="ONT64_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2303753.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3599..5083)
                     /locus_tag="ONT64_002357"
     CDS             complement(3599..5083)
                     /locus_tag="ONT64_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR2303754.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5103..5564)
                     /locus_tag="ONT64_002358"
     CDS             complement(5103..5564)
                     /locus_tag="ONT64_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2303755.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5582..6649)
                     /gene="ulaG"
                     /locus_tag="ONT64_002359"
     CDS             complement(5582..6649)
                     /gene="ulaG"
                     /locus_tag="ONT64_002359"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR2303756.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6867..7637
                     /locus_tag="ONT64_002360"
     CDS             6867..7637
                     /locus_tag="ONT64_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR2303757.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7910..8737)
                     /locus_tag="ONT64_002361"
     CDS             complement(7910..8737)
                     /locus_tag="ONT64_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2303758.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9305..10540
                     /locus_tag="ONT64_002362"
     CDS             9305..10540
                     /locus_tag="ONT64_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCR2303759.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(11539..11799)
                     /locus_tag="ONT64_002363"
     CDS             complement(11539..11799)
                     /locus_tag="ONT64_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR2303760.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(11826..13127)
                     /locus_tag="ONT64_002364"
     CDS             complement(11826..13127)
                     /locus_tag="ONT64_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2303761.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4220 a         2764 c         2080 g         4220 t
ORIGIN      
        1 agtgaactac ttaagcaatt tccacaagaa gaacatttca tgtataaaga agcaatttcc
       61 cacaaagtga cagagtattt ggagcataat ttttttcgta taaagccagt gcgtactcgt
      121 tcggcaaaag gaatatacga gatgaaagtc aaaattaata aaaaaaatta tcgcatagca
      181 tttgctttaa aaaaaaatca tgtgttcatt ttttatatta gttcgaattt gcaaaaaatt
      241 gtatttgata aagaggtgag taaaaaaata aaatagctga gcattggagg atagattgag
      301 tgatactaag tctatcctct tgtttttcta aagaactaag ctagaatttt ttcttttgta
      361 cgttattata tgttatttgt cattaccaaa aagcaccttt tttcttctag tatctatgac
      421 ataagaaata ccacccattt cctttacttg aacaaatcct actgattcat aaaatttttt
      481 tgttttctca gtatcatctg tcaataattc gatttggact ggtccataac tagtgatctt
      541 ctccaaagct tcattcaaaa gcactttgcc tattccttgg cgctgatacc tcggatgaat
      601 gaggatatct tgaataaatg cgatcgtcgc aaaatcagtg atccaacgaa cgattccgat
      661 aatcttccct tccccattac gctttatcag agattcagat tgttcaaatg ctctagctaa
      721 ccttgcagtg tcttttgtgt aattcgtcca accaacgctc ttatataatt cagaaatctc
      781 atcgatcata agggaatcaa tattcatttt tttcttcctt tcttatacaa ttctccctct
      841 aataatacag ttctttgact atttaatcta tcacatataa ctctcctgta aagattctct
      901 cgattcctta aaattcctct ttccttttag aatgcatact ttttgataaa taaaaaggtt
      961 gtgataaatt ttcttcacaa cctctgtttc tctctatcaa gtgttatctt ttaattttct
     1021 acccttaccc tagcatctct attgcccgta gtatgcatac tttccatgtt tgcgaaaata
     1081 atgtttatct agtaaatatt gcgggatttc acatatattg gggttgatca gctcgttttc
     1141 tttggccatc agacaaattt catctaagat caaactgttt tctactgctt tcataggtga
     1201 atccccccat gtgaacggtc catgtccata gacaagtact ccagggactt cgtttggatc
     1261 taattttctt tccttaaacg tctcgacaat cacattccct gtatgaactt cgtaagcttc
     1321 tcttacttct tctttcgtta attggcgtgt acaaggaacc ttaccataaa aagcatcagc
     1381 atgagtcgtg ccataagctg gcaaatcgcg tcctgcttgc gcccacatga cggagtgcgt
     1441 ggaatgagta tgagtaatag cagttatatc ttcaaaagtt tgatacaaaa caacatgtgt
     1501 ggggagatct gaagatggtt ttaagctatc ttcttctatt atatttcccg acaaatctgt
     1561 taccaccatt tgatctgctt gcatgcattc atatctgaca ccgcttggct taatgacgat
     1621 gactcctagc tcacggtcca cttcactcac attgccccaa gtcaatttga ctaatccgta
     1681 tcgcggcaat gccagatttg cttcaaggac acgttgcttc attgctgcaa ttttttcttc
     1741 acaagacatc atagccagcc tccttcaatt tagaacttaa gtagtctttt gcctgctgga
     1801 tttctcttcg aaagtcttgg cttttttcac tccacatttc aatcagaaat gttccatcat
     1861 aatctagacg tttcaaatgt cttaataatc caagaaaatc aacacaaccg ctaccaaaag
     1921 gaacatcacg aaactggcct gaatattctt ttgttacagg gtaagtatct tttaagtgga
     1981 tagcagtgat ataatcgatc cctttctcaa gttctcgagc cggattattt tccggccatg
     2041 cggacaaatt gcctaggtct ggatatactt gtagaaaagg tgaatgaatc tgttctttga
     2101 tttctaaaaa ttttgaaatg gagttcataa atggatcatc catgatttcc acggaaagaa
     2161 tgatgccata tttagaagct tcctttacgc tttcttttat tccttcgata aagtcttctc
     2221 tggaattcat tgatttctct tcataataaa catcgtatcc agctatttgg attactttga
     2281 cgcttagatt atgcgccaaa tggattgctt ttttcattag ttgtttcgct gcttttttct
     2341 tttctggatc agcagaacca aacggaaatc gccgatgcgc acttaaacaa atcgaatgga
     2401 tacgtacacc ggagctgaac atctcttctc tcagctcagc tatcttttct tcagaccaat
     2461 ctaatcgtgc taaccgctca tccgtctcat caatcgacat ctcgataaaa tcgaagtcca
     2521 tttcttttac aagggaaaaa cgttctttcc atgaaatatc tttgggtaac gctttttcat
     2581 aaatcccaat ttgagccatt tccttacccc cagatacgaa tgatctcatc tttgaagttt
     2641 tgtgcggcct tcatcggatc atttgctgct gtaataccac gaccagtgat aaaggtatac
     2701 acatcgatcc cttcaaataa gtgtaatgtg tgtggattta atccaccagt tacagaaaca
     2761 ttaaagccta gttcaatcag ttttttgatc ttcgataaat ctttttcacc ccaagtttca
     2821 ccagataaca gcgcatcacg gctttgatgg tagattacct gccggatacc gatgttatgc
     2881 caatccatgg cttgttcaaa agtccaattg ccgtagagtt ctacttgcaa ttcacctatt
     2941 tctttttgtg ctgcctccat agttggaatc gttgcacaac aaatgactgt catccagtct
     3001 gctcctgctt ttgcaacatt actcgccact gttcctccag cgtctgcaca tttcgtatca
     3061 gcgactagtt ttttatctgg gaacatactg cgaatacagc gaatcgcttt ttgtccttcc
     3121 tgtaagcaaa gaattgtgcc cacttcaata atatctacta catcaccaac tttcataaca
     3181 tctcctaatg catgttctaa gtcattgtga tctaacgcaa tttgcaaatt cggcctgctc
     3241 atcttagttc cttcctttac ttgttttttt ggtaccactt tgtttcagca aatttttcac
     3301 tgatttcttt gactgacatc acatttttga taccgataac tgtaactcct tttttctctg
     3361 cctgagtaaa catattcaaa aaattgactg gcgtaaaaac tacatcgtat tggccagctg
     3421 aactttttcc ctctgaaatg gcacaatgat ggattttggt aattggtacg tttagttctt
     3481 tcattgcttt ttccacactt ctcatcatca tcaagcttgt tcctgatcca tttgcacacg
     3541 atactaaaat tctcatctta atccctccat tatgagtgtt gtctaatcag attttctttt
     3601 atactctctg cttttctgca tatgtgtcat agtcttctac aatcaaaaaa taaccatcag
     3661 gatttttccg atattgtaac tgcggaatag ccaataaacc aatgacaact acggcaattc
     3721 cagcataacc taggactttc atgattactg taaacaatgg ccaagctgtt gcccagtcga
     3781 acatcccgat ataaccaccg tatgttgaca tcccaatcca tgtagcgaat aatgctgaac
     3841 cgcctacttg tatgattcct gaaagaaacg ggaacaggca agcggctttg aatccacctc
     3901 ggttattggc ataaactgct ataacggcat tatcaaagaa caacggaatg aaaccggcta
     3961 tgacgatcgt tggtgatttc aataaaatca gtaaaccaat catcaaaaac tgtcccaaag
     4021 caccaaacaa gaacccaatc gttaccgcat ttggcgaacc aaagccaaaa gtagcagcta
     4081 cgtcaattcc tggaacagct cctggtaaaa tcgtgtttga gattccttgg aaagattgag
     4141 ttagttcatc gacgaatgtc cgaacaccta attgcaagat cgccagatat accgcaaaat
     4201 ttaaagaagt cgtcataatg tagaataaga aactctgccc ttcttgcatg aattgcgctt
     4261 gaatcaaata atctttgcct agtacaatca gaatgatccc aaagaaaaat agcatgagta
     4321 acgaagtagc aaccatattc tcattgaaaa tcgacaagaa tcctggtaat ttgatatctt
     4381 caattttttt gtcttgcttg attgatctat ttttcttttc atcccgcttt ttcatccact
     4441 cagctaattt tgcaaatata aagattccga acatttgctg atgtgccact gcaaatccgg
     4501 caccttctgt caattcctgc gtgattccta ctgttaagtt agaagctact gtccaataac
     4561 aacctaaaat cagtcccata aaaatcaatg cttgtatcct acctagatca ggaaagcaaa
     4621 acagtaaaat ccaaaaagcg gtagctgctt gttgaacctg aacgtttccg gtagtgaaaa
     4681 cggaacgcag tttcgtatat ttctgaaaac ggactaataa gatatttaaa acaaatgcaa
     4741 tcagtaataa aatcatcaca tctccaaagg tacggccaaa tgtctccatt aatccagcat
     4801 ccacagcatt ttgtccaaaa taaggatctg taaccatggc atccaaattg aaccgttcct
     4861 ttagtccaac caagattggc cgaaaattat tgaccaaacc accagagcca acagtcaaaa
     4921 taaagtaacc gaccgttgcc tttaagaatc cagcgatgca ttcatgaagc gggcgtttca
     4981 gcaaaatata gcctaataac acgataaaac caatcaggta tgctggctgc gtcaagatat
     5041 ttgttgcaaa atacgtccaa atgcttaaca aaaattcccc catctttttt ctcctctatc
     5101 aattaattat attctgccat cacaacttgg taatcatcga tggagttcgt agagatgaga
     5161 cgctcaacca atccatctgt cattaataat tcggataatt tttggatatt ttctagatgt
     5221 tcttctggat cttttgctgc gagtgtaaaa aacaaactag ccgttttctc ctcattctcc
     5281 ccttcacttt caaaacggat tggctgtttc aacttggtaa atgaaatagc tgttccaaaa
     5341 acaccatcac ttttttctga agaatgcggc attgctactt gaggtgcgat cacaatataa
     5401 ggaccgtatt tttctacaca atggatgatc tcctccacat acgtttcatc aataatttcc
     5461 ttatcgatca atacaagaca gctttcggcg atagcttcac gccaatccgt gatttcttta
     5521 tctgggtaat gaatcaattg atggtcaaaa aagtatttca gcaagaaact ccccttcttt
     5581 cttataaaaa cgacggaaac ggtgtatcat tttcaatcga gaagacatct tcaaatcctc
     5641 gataaaaatt aaactcaaga tcatctttgt ttgtcggata gatgaatttc ccgcctactt
     5701 gccagatata aggtttaaat ccatacttca ggcgatcctt tttgaatttc caaatttcag
     5761 tgatctcttt tggatcagcc ataaaattag accaaatatc gtagtggaca ggaatcacta
     5821 cttttgcatt caatgactcc gccatgcgca gcatatcaac agaagtaact ttgtctgtta
     5881 tccctcgtgg gttttcacca taggccccta aacagacatc gattttgtgt tcattaccat
     5941 gtttggcaaa taaattggag taatgtgaat ctcctgcatg ataaagattc cctcctgatg
     6001 tttcgaataa atagtttact gcaatttgat ccatctcctg aggcattttt ccttttaata
     6061 cctcttctgg atcttcacaa gtgaccaacg ctgtccgatc aaacgcttct aaagctacta
     6121 cattgatgtc tttgattttg actgcatcac ctggctttac tgtaatcgtt ttttcttcag
     6181 gaattcccca tttttgccac gtgcgaacca cttcttttgg cccaatgaat gttgcttcag
     6241 gacaattttg atgaactgct gcggctgtat ggatatctag atgatcggaa tggatatgtg
     6301 taacgattaa agcgtctaca ttttttatcg caaaaggatc gattacgaaa ggctgagtac
     6361 gtaaattagg ctgcatattt tgaacgccac tcatacgcat catctgatgg ccttttttca
     6421 tcatgccatt cccatgacta cgttttcccg tcccacacca tagatcacat aaaatattcg
     6481 ttccttcatg acttttcagc caaattcccg tacagcccag ccaccacata gcaaaagttc
     6541 caggatccac tacttctgtt tcgatttctt cattcaagta tgtgccccac tcaggaaatg
     6601 tttctactaa ccacttttct tttgttactt cattgacagt tttactcatt tgcttgctcc
     6661 ccccatatcg ttgttccaac ttacaagtaa atattaatgt gtatcagcat ccatgaaaac
     6721 ccttttatat gaccgtttgt tttttatatg attttctttc agtaatcaat gattgtttca
     6781 gtataataaa gacagatatg aatgtttaaa tgaacaacta gtcaaataaa tccatggatc
     6841 aagatgtccc tcaggaggca aaagtcatga aaaactcact tgtcaatatt gaaaaacgtc
     6901 aaaaagaaat tttggaactg cttcagcaga ctgaacaact atcaactttc gaattagctg
     6961 aatcccttga tgtatctatt agtacgatac gaagagattt aaatttatta gaagaaaaaa
     7021 acgagatcat acgcaattac ggttattgtt cttataatta caaaaaccag actgattttg
     7081 atcaatctgg tcctgaaagg attaaacaag cgattgccag agaagctagt aaatacatta
     7141 acgattatga tacgctgttt atcaattcta gctcgactgc gttgaaaaca ctggattttc
     7201 ttcaatctaa ccatttgaca gtcattacaa acaacgtgaa aatcaattac tctccgcaca
     7261 aagataattg tagttatatt ttaactggtg gagagatgcg ctttccaaaa gaagcactag
     7321 tgggcgacat cgcattaaat acgatttctt cagtcaatgc agacatttgt atcattggct
     7381 gtagcggagt cgatgtagta catggggtca caacaaagat attaaatgaa tcaacgatca
     7441 acgaaatgat ggtcaacaag acaatcaaat gtaagatttt agtcgctgat catcgaaaaa
     7501 tcggactgac atcaaagttc aagattgctg atatttcagt ctttgattat ttgatcactg
     7561 ataaattttg ttctgaaaaa gtattagagg agatcagaaa tacggggata aaaaccattc
     7621 aagtagattc attttaagaa ccaggacgta aatatgtaaa taggattatc aaaaaaatga
     7681 ataacaaatt tatcgataaa tgaaaaacac gctacgctca aattgataaa ggaatcccaa
     7741 gatgtatcca tccaatcgat accatcataa tctattgctt cttttataac cgttatatgt
     7801 tgcctaaata ggaatcaaac aatatcgaaa aaaggaggta tgacaaaagt cacacctcct
     7861 tttttcgaat aaacggtatt caaatcagaa cactgatttc acaaccactt tatttcaggt
     7921 cgttttcaat cacctttacc gcgtaatcat ttccgttgaa acgcgctatt ttttttgcgt
     7981 ccatatatct ttctaaataa tcatcttctc ctaattgctt atggcaagaa gcaatttcat
     8041 aaagcaagta ttccaatgca taagtcgatt tatgtttctt attatactca atacccttct
     8101 cacataattg gattgcttta tcataatcct cgattttacg gtaataggtg gcgatatcat
     8161 aatatacaat gcagaccatc agattatcaa aatcagtctc actcaatatt ttaagtgtac
     8221 gctccacata aactttcgca aaatctaatt ctccttttga caagtagatc attgccacgt
     8281 ttgcagctgc ttgtaagtga tggatatctg tttcggtcgt ttgttccaac acttgattaa
     8341 aataaaataa tgctgcatct ggttttttcg cataaagatg atcaagcagt ccttgataaa
     8401 ataaaatttg atttaaaaga tcagaattac ttactttttc ctgatcgatc gtttgtaaaa
     8461 ttttataaga ttcttggtat tttgatacag aattcaatga ttgaatctca tttatttttt
     8521 tcctgatttt gtcttcttct gtttctacta atatatcgga gatctcgaca ttcagctttt
     8581 tgcaaagcgc acttaagata ttgatgcctg gcgacatatt cttgttttcg atattgctga
     8641 cagttgcttg ttcacaaatg ccttctgcta attgtacttg tgttaacccc tttttctttc
     8701 ggtattcttt tagtaatgct ccatctactt taatcaaaac aatttccttc cctttaaaaa
     8761 attatttctc ctttatatca tacacaaaaa aaagttctaa acaaccaaaa atgtttagaa
     8821 cagaataaaa attttaattt taaaataata ttcaaattaa acaactggcg tatctgctcc
     8881 ttgatttctt tttgtgttat ctgaataatt atcgtttaca gttataacac atagtgaaaa
     8941 ttgtcatggc gagaaagtct aagaatattt ttaaacataa ctatttcttt cttacgtgtg
     9001 tataatgacg acagcacacc tatcaattaa tataattatt ttattttttt atgttctcat
     9061 gtttattttt aatgacacaa cttctcgtat ttatctggca caacatataa atcgatcaaa
     9121 aaattgttgc gtgttttttt gaaaattgga gtttcaactt gattacaaaa tttaaattta
     9181 gaagttgtga aaacagcgag agacagttta atattcaaaa tatacatagg ctactcgaac
     9241 gctgtgtttt ttagtatact agtaagaaac gaagggatag actcattcca ataaggagga
     9301 tttcatgaag acaattcttt ttgctcctgc agtattcaat ttagctgaga caacacgaat
     9361 gattgaggtt gccaaagcta tgaaagaccg ttattactgt gtattctttg gcttttctaa
     9421 aacttttgct cctttgatca cagaaagtgg ctttgagtat catttactag cgccgatcct
     9481 tacgaaaaaa caagaagaac aaatcataaa atttgaccag ttgcgtacac ttaaaaaccc
     9541 ttttaccaaa gaaatggttg cctctcgtat cactagtgaa cagagtctga ttgctaagtt
     9601 tgagcctcga ctgattgtga caggttcgaa tgtgacaatt tttttatcgg cccgcaataa
     9661 aaagattcct ttagtatatg ttaaacccta tgctttgagt gaaccttttt ttcaatcaga
     9721 gcatgcgctg atgccccacg agttacgttt cttcggccct ttaagcaatc ccctttggca
     9781 ctggacaaaa aagcgtatag taaatacccg atggatcccc aatgccttca aacacgctgc
     9841 acaaaaagaa atgcttcctt taccaaaaac aagtttacaa atgatggatg ctgatctaaa
     9901 tctcatcaca acgcctaaac tatttttgga agaagaacag ctgccggaaa attatcgaat
     9961 cgttggtcca atttttgcta agctcagtac ttccttgcct gaagaagtgc taacttttat
    10021 acagcgaaag caacaagaaa gaaaacgtat tatctatttc gcaatgggta gttcaggcaa
    10081 tccgcgtatg ttacgcaaaa ttttgttctt cttacaaaaa caaccggatc tagcggttat
    10141 tgcacctatt gactatctgc ttgatgacat tcaatctgac tcagaagatc ttttaataac
    10201 agaatatctg tctagtcatc ttttgcatga attcattgat ttttctttta tccacggcgg
    10261 ggaagggacc gtccaaactg cctgtgaatc cggcaaaccg tttatcggtg tggggatgca
    10321 atttgagcaa agctgtaata ttgactattg tgtaaaatac ggaaacgcaa tcgccctcac
    10381 aaaaccagtg actgaaagaa aactgcagat tgctctgcgc gaagtttctt cacccaagat
    10441 tcgtcagcag gcacgcacgc tgcaagccgt ttttccaata aatggtccac aacaagcaat
    10501 tcaagaaatc gaaaccttcc tgagcaacca cccaatatag tttcaagtag aaaaagcttt
    10561 tttaatagag actcatcagt aatttcgcta ttcgagaccc agacagtgaa aatacaaaaa
    10621 aagtaaatag tggattgaaa agttagagtt acttaaatta cggtgtctaa aaaacacaaa
    10681 atcatcctta aaataaaaga tctgacgcta tccaaatgag cataggtagc gtctgccatt
    10741 tattcttagt caaattcttt gacacctttc tctggttgga tcatctcaac gtttcttcaa
    10801 aagaaagacc ataataatta ggcaaataca ttttcttaaa atgtgtcata tagatctaga
    10861 agcatcgctt tgtagagatt tgtgccgcta gtcccctcaa caattactaa atcaaatgta
    10921 ggatttacca ctttatcttt gataatcaac ctccctcttc tgttgagaca atgatctcgc
    10981 aatccgatgc atactcctat ataaactgcc agcaatcccc acatattgag tagatcatgt
    11041 cagaatgttc tatgtcgtca gcaacgatac actaaaagtc acgttcacca tgactgattg
    11101 ctattcccag cacaattatt tctatacacg tttcatgatt tgaataaaca ctcacatcat
    11161 acatattacc attcatacac catactgcag cccctactat atggttgtaa gctcccaaat
    11221 ctatatttga cttgatcact ttggtagtag atctactaat tctaaatcca ttgctgataa
    11281 aatttccatt ttttcctcct tctttctgcc aaaacagtta aaacaataat aatctttatg
    11341 taaaaaaggg ctgtgacaaa agccttgtca cagtcccata ttccgaataa acggtggtca
    11401 aaagcttgca tctgcggtaa taagttcaac caaacgaaaa ataagccaac atttcacaaa
    11461 aatttaagaa gcaattttcg taaatttctt cttatttctt gaagctaacc acttctgtta
    11521 caaccacttt tagaacattt aaagcttcga tgtgacaaac catctatgct gctcgatcat
    11581 aaaattgata gcaaaaattc cacaaagtac atatgtcccc catgaaaaca aaacattcat
    11641 atgcggtaca atgatctttg gaagaatcac gatcaaaaat gatgccagtg cccaaatcca
    11701 tcgattgatt tgaatatgat atgtagctag catattcaat ataaggtgac tagccgttaa
    11761 taaaaagata aaaacaaaaa ataaaatcag ccaatacatt gtttcccacc ttctaaaaac
    11821 caaacttaga cagtctggac ttcgttttct ccacgacttt gttcatctga cagcatcttt
    11881 ttatcataag cttttacaaa tggataatag ataataaatg ctatcaatgt gttgatcatg
    11941 atcaagataa caacacgcca gtcatttcct gctgaaagat aaccgccgat tggagctggt
    12001 aatgtccatg ttggcaaaac agtaaatcta ttgaccattt ctaatttgac tgcaaaataa
    12061 ctgataatag tcaaaactaa aggaactaag ttgaacggga tgaacatgta aggattcaac
    12121 atcataggag ctccaaaaat tatgggctca ttgatattga agataccagg aataatagaa
    12181 aaacgaccaa cctctttcaa gaattttgat ttacagaaga atataaacaa aacagatacc
    12241 acgatcgttg ctcctgcacc accaattgtt accgtccatt gataaaactg ttcaggagcg
    12301 atatagggta attgggtggc agctgtacca tctgccaatg cctttgcatt ctcatccaac
    12361 atgatcagcc acattggacg aacaacagcc cctacaatac tcatgccatg gataccaaat
    12421 gaccacaaca aatggatcaa aaacattggt agtaaaacac caagcaaatt attccctaaa
    12481 atattggtta aaggctcgaa taacgataat aaggcagcgt ttacatcaaa gcccgcgacg
    12541 cttcgaatca gccaagtaat tgttaaaatg actgcacctg gaatcaatgc ctcaaaacta
    12601 cgggcaacag caggtggcac ttgttctggc attttgatag taatatttcg atttttaaag
    12661 aaacgatata cttccactgc aaaaatcata gataagatcc ccgcaaacat cccagatgct
    12721 cccaaagaac tcataggtaa gacccagcca agaggtgcct cttcaggagt taaaacgttt
    12781 acaggaactt gtaacataaa gaacgttcct aaagataata taccacctgt cacactatct
    12841 aatccatatg atttagctag atccgaaccc ataacaaaag ttgcataaat agccatcaat
    12901 cccatcgtca tcctaaaggg taaaacaatc gttgccgaat aaggggcaat aaattcattc
    12961 catccaggaa acggtaaatt gataaagacc ataaaaaatg tcccaatcaa aatcaatgac
    13021 aatgttgaaa caaccccatt acgaattgct aataaatgac gctgtgtacc aatttttgcc
    13081 attggtggga caagtttctc ttcgacccat ttcatcagct tttccactaa aaccactcct
    13141 tttcaataaa atatgaacca ttcattgcat gacaacgcaa agaatgttca taactaatta
    13201 tataattaag aaattttatt tcaatatatt ctcttaaata atcaaattta tttttaagaa
    13261 tcctgaaaat aagttttcta aatc
//
LOCUS       DAJSAW010000071        12734 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJSAW010000071 DAJSAW010000000
VERSION     DAJSAW010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.071"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ONT64_002365"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ONT64_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR2303762.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ONT64_002366"
     CDS             complement(322..891)
                     /locus_tag="ONT64_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCR2303763.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ONT64_002367"
     CDS             1077..1979
                     /locus_tag="ONT64_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2303764.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ONT64_002368"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ONT64_002368"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR2303765.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ONT64_002369"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ONT64_002369"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR2303766.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ONT64_002370"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ONT64_002370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR2303767.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ONT64_002371"
     CDS             8547..8732
                     /locus_tag="ONT64_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCR2303768.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ONT64_002372"
     CDS             complement(8794..9990)
                     /locus_tag="ONT64_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR2303769.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ONT64_002373"
     CDS             complement(10160..11596)
                     /locus_tag="ONT64_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCR2303770.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ONT64_002374"
     CDS             11889..12698
                     /locus_tag="ONT64_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2303771.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2675 c         2249 g         4007 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaacgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DAJSAW010000072        11130 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJSAW010000072 DAJSAW010000000
VERSION     DAJSAW010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.072"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            137..310
                     /locus_tag="ONT64_002375"
     CDS             137..310
                     /locus_tag="ONT64_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303772.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            950..1423
                     /locus_tag="ONT64_002376"
     CDS             950..1423
                     /locus_tag="ONT64_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HCR2303773.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1432..1659
                     /locus_tag="ONT64_002377"
     CDS             1432..1659
                     /locus_tag="ONT64_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2303774.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2295..2666
                     /locus_tag="ONT64_002378"
     CDS             2295..2666
                     /locus_tag="ONT64_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303775.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2922..3164)
                     /locus_tag="ONT64_002379"
     CDS             complement(2922..3164)
                     /locus_tag="ONT64_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR2303776.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3196..4098)
                     /locus_tag="ONT64_002380"
     CDS             complement(3196..4098)
                     /locus_tag="ONT64_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR2303777.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4111..4299)
                     /locus_tag="ONT64_002381"
     CDS             complement(4111..4299)
                     /locus_tag="ONT64_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCR2303778.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4313..4876)
                     /gene="amaP"
                     /locus_tag="ONT64_002382"
     CDS             complement(4313..4876)
                     /gene="amaP"
                     /locus_tag="ONT64_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCR2303779.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4914..5807)
                     /locus_tag="ONT64_002383"
     CDS             complement(4914..5807)
                     /locus_tag="ONT64_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2303780.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5885..6807)
                     /locus_tag="ONT64_002384"
                     /pseudo
     CDS             complement(5885..6807)
                     /locus_tag="ONT64_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
     gene            complement(6856..7206)
                     /locus_tag="ONT64_002385"
     CDS             complement(6856..7206)
                     /locus_tag="ONT64_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR2303781.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="ONT64_002386"
     CDS             complement(7239..8141)
                     /locus_tag="ONT64_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCR2303782.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="ONT64_002387"
     CDS             complement(8134..8991)
                     /locus_tag="ONT64_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCR2303783.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="ONT64_002388"
     CDS             9325..10134
                     /locus_tag="ONT64_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2303784.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="ONT64_002389"
     CDS             10174..10671
                     /locus_tag="ONT64_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR2303785.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11056..11129)
                     /locus_tag="ONT64_002390"
     tRNA            complement(11056..11129)
                     /locus_tag="ONT64_002390"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11093..11095),aa:Ile,seq:cat)
BASE COUNT         3255 a         2085 c         1872 g         3918 t
ORIGIN      
        1 tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc gttcttactt
       61 tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc ttataaataa
      121 aaacgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa cgattggcta
      181 tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat tcagttggac
      241 tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac aaaattatcg
      301 aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta tggtactttt
      361 tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac ttttgattta
      421 taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga tagtaccacc
      481 ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata aaggacaaat
      541 aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa taaacatgaa
      601 cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct catacagttc
      661 tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta ttgtccaata
      721 taatcccagg catctaaact agcattatag aagagacaca catagaatct aaaataactg
      781 gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac cttcatactc
      841 tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag ttgttttaca
      901 ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa tgaaatttaa
      961 aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt tgttcggcgt
     1021 acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt ggcagaccac
     1081 attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg cttatttctt
     1141 attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa gcttaaacat
     1201 actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg gcttacttcc
     1261 taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg taggaatcgc
     1321 tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa aactgcttga
     1381 acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga catggcaatt
     1441 atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga attgagtgag
     1501 aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc aaaagccatc
     1561 cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg tgatttgttg
     1621 acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga acaaacagta
     1681 aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt acgtctatta
     1741 ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat gtactgactc
     1801 atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca actggtttcc
     1861 tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca ccagactgat
     1921 agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt tcataccttg
     1981 aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat tcttttttag
     2041 taagctccca tatatactgt aaagccttct atatattatg tcatgttgct ttttaacatt
     2101 tactatgtag ttttgataaa atgatttgaa taacagtgat ggacaattct tttttatttt
     2161 taacattatt ttgataataa tgattattta tttaaaaata cattagataa catttgttac
     2221 gacacgaaaa agcttcacaa acaaatatcg ttattttaaa atgagggcga actcacacaa
     2281 ttaggaggaa tagtatgcca tttattgttt acttttttct ttcattactc gtgatctata
     2341 ttccattacc aacatttttc ctgctcgtca atcgtctagc agagcaacat gatacgattg
     2401 ctgctcttac gaaactttgt ttacttctat ttgtttgtct agattacttt acgagctatt
     2461 attggctaaa acagacaaga gtaaaaaaga gatattattt cttccttgtc tttttcaatc
     2521 tctttgagtt tctgatccat tgctttttac ttcatcagta tcatacgatc catttgtgga
     2581 tcatctgggt tttcaaagtg gtcgtattat tttgtatgtt cacctttcct atctcaagaa
     2641 aatttaggga ggcattgttt tattaaagaa gcttctctgt atcaataaag acacatttaa
     2701 ggaaaaactt ttaaaaatct aattgggaat ttgcccgcat aaataagagg ctgtgacaaa
     2761 agtcttgtca cagcctctta ttccgtataa acagtgttca aaagcttgca tcttcgataa
     2821 tatgcccaac caaatgaaaa atatgagaag ctatttttgt ttggctgttc ttattacttg
     2881 atgaagaaca gctttttcac gacctctttt taaaagtctt tttacagttt tcgagcaaag
     2941 aatgatacga ttagtactaa aatgatggca ccaatcaatg atggcacgat tgccattcct
     3001 gcaatacttg gtccccaaga acctaataat gcttgaccaa gtgctgatcc aactaaacct
     3061 gctacgacat ttgcaataat ccccatcgaa gaacctttat tcgtgattgc tcctgcaatt
     3121 gctccgataa ttcctccgac gataagtgac caaataaatc ccatgtttcg ttcctcctaa
     3181 tgttttactt ccttattact gaacccgtgg ttctgtattt tcttttactt tttgtgcgcc
     3241 gcttccagct gctgatttta ctttatctgt ttgattcgaa gcaaattcac cggttttttt
     3301 cgctgcgtca gttactttgt cttgcaccgt ttcttgatct ttttcaaatt cttcttttgt
     3361 tttgatgtcc actacgttag cgttgacttc gatcacttct aaatgtgtca tattttttac
     3421 ttctttgccg attacttctt gcatttcttc aaagattttt tcgatatctt tgccatattc
     3481 cacaatgaca tccatatcaa ctgctacttg ttttttaccg acttctgttt cgatcccagc
     3541 cgttacatta tctgtattca caagttttcc tgccacatta gagaagaaac caccgtttgc
     3601 agataataaa ccgtctactt gttcaattgc gattccgatg attttttgca caactttatc
     3661 ttcataattc agctcaccgc gcattgaatc ttcctgttca gaatcatgct ttccttggtt
     3721 atttgtatga tcagaaccaa gttttccttg tccgtttgtg tggtcagaac taagtttttc
     3781 ttgtgctttc gcatggtcag aaggatgttt ttgctcttcc acaggtttag aaggatcgat
     3841 tgcatcatgt tgttctggaa ctttagattc tggagttcct ggatgatgag gtgcatgctt
     3901 ttctgcatca tgtttcttct cttctactcc ttgttgaacg ttagggacta ctggatctgg
     3961 atgtgcagat actggtgctg ttttgttctt ttcatcgttc acaccatttg attggtttgg
     4021 tgtcgttggg tctggatgtg cagacactgg tttttggttg ttcattttat tgttattatt
     4081 tttgttattg ttattcatgg atgtttcctc ctaagattta aaatagtgat ctaagatttt
     4141 cgtttgtttc agataaaaac cgatagcgac gccgatcgct gccatgatca agatcaataa
     4201 ggtcttgaaa aagcccacgg tgatccataa aacggctaat atcaatccaa gtaccccaaa
     4261 gatgataggg agcttgtatg tcgaaaatag ttctttcatt tttggtcccc ccctattcta
     4321 ctcgtttgcg gtcattgctt cgtttagctg gttcttcaaa gcctgtgtat ttcaccgata
     4381 ctttcactgg ctcttttgtt cccaatatgt gttgtacttc attttcgatt tctttcatca
     4441 atgttcctgt tttaccaatg agtgctgaag ttcgtttcaa ctcccctttt acattgacgg
     4501 cgatcttgtt tttcgttgct ttgacattta cttttggtga atggatgaat tcatcttcat
     4561 gcaagtgaga acgaaccatt ccttcaattg cttttttatc caacgtcaac tccccatcac
     4621 catgtttcag tagaaagttt ccgattgttt ttggataaaa caggaaaaca agtaacgtga
     4681 taattgtcaa aatagcaaat gcaacggcaa gccaaaatac atattgttgc acgtatgaat
     4741 tagtgatgga aaagaatcga aacttctcta gctgaaaagg caatggatag gttgcactta
     4801 atagtatcgc aaacaaaagc acagatagca aaagtaatgt tccgatcact ccgatcgttt
     4861 ttacaccttt attcattttc gcacctcctc ataggggtga aacgatcaac ttcttataag
     4921 ttgatcgctt ctccaccagt tactccgtaa atttgggctg taacataact tgctttattc
     4981 gatgctaaga aaacataaac tggtgctaat tctcctggct gacctgcacg ttctagtaaa
     5041 ctttgctgac caaattcagg gatcgaatca gaaggttgtc cttcgtctag ttgtaatggt
     5101 gtccagattg gaccaggtgc tacaccattt acacggattc ctttaggtgc aagctgtttt
     5161 gctaaaccga tcgtaaagtt agcgattgaa gcttttgttg ctgcgtaatc taataaatgt
     5221 tcgcttggat tgaatgcttg aacagaagtt gttgttacga ttgctcctcc tgctgggata
     5281 tgcggaacag cttctttcac taaagcaaac atagcgatga tgtttatcat aaatgtgtct
     5341 ttgacttgct caagtggtaa ctcagcaata gactgacaag aaatttgctg tcctgcgttt
     5401 agtaccatcg tatctagccc gccaagttca ctcacggctt tatctatgat ttctttaggt
     5461 gcttgttcgt cacgaagatc aaaaggtagt aagacaatct tacgtcctgc atctttcacg
     5521 tattttgcta cttcctctgc atctttttct tctcctggga agaattgaag agcgatatct
     5581 gccccttctc tagcaaatgc aatcgctgct gcacgaccga tccctgaatc tccaccagtg
     5641 attagtacac ggcggttttc tagttgatta ttgccttcat aggattcttc tccacaatca
     5701 ggaacaggaa tcatattctg ctgtaaagct ggtgtatcct gatcttgttt aggaaatttc
     5761 tccgtatggt acaagtttcg cgggtcgatt aattttggtt ctgtcattgc tgtattcctc
     5821 ctagttttta aattgtattg ccaaaacaaa tttctttgtt tgatacctgt tttaaataga
     5881 ttctttaatt tgtataatct ttccggtata aatggtaacc taaaactact gctgctatca
     5941 aagagccaaa gactacgagt ataaagccta gagcagaact tttacccggt aatcctcctg
     6001 tattcattcc ccaaatccct gcgaacaacg ccggaacgga aatgattaat gcagctgaat
     6061 ctagatattt catgatatga tttagattat tgtccatcat cccgttgaat agatcaccaa
     6121 tactctctaa taaatctcga tagatctcga tcattttttc tgtttgtcgc tgtctcaaat
     6181 ggacatcata gagtagctgt tcatcgtcta aacgtttgat gaaagtttca gaattccata
     6241 attgatccat tgtttcccgt tgatctctta aggtatgatc gatatatacg atgtctcttt
     6301 caagatctgc ctgtttatac agttcttcat tttccgttgt tttacgagct gctttatcca
     6361 aagcgtcaat tcgctttttc atctctctta gttctttcac gtaatgggtg taaatatcta
     6421 ataatgtata cgtgatgatc ttttcaaacg agtttaaatg atttccatgc tttgtcagca
     6481 gccgatccac aaattcggtt tttctatcga tacaagtaat caagagatta tcggaaagta
     6541 caaaagatat tggcgttaat ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10981 gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    11041 tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg ttatgagccg
    11101 tctgctctaa ccaactgagc taaaggtaca
//
LOCUS       DAJSAW010000073        11031 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJSAW010000073 DAJSAW010000000
VERSION     DAJSAW010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.073"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            131..505
                     /locus_tag="ONT64_002391"
     CDS             131..505
                     /locus_tag="ONT64_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR2303786.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ONT64_002392"
     CDS             complement(619..1248)
                     /locus_tag="ONT64_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR2303787.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ONT64_002393"
     tRNA            complement(1382..1454)
                     /locus_tag="ONT64_002393"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="ONT64_002394"
     tRNA            1668..1741
                     /locus_tag="ONT64_002394"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="ONT64_002395"
     CDS             complement(1879..2043)
                     /locus_tag="ONT64_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303788.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="ONT64_002396"
     CDS             2338..3666
                     /locus_tag="ONT64_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HCR2303789.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="ONT64_002397"
     CDS             3868..4701
                     /locus_tag="ONT64_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR2303790.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="ONT64_002398"
     CDS             4717..5454
                     /locus_tag="ONT64_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HCR2303791.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6535)
                     /locus_tag="ONT64_002399"
     CDS             complement(5609..6535)
                     /locus_tag="ONT64_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR2303792.1"
                     /translation="MKENMLTGGVSPDVANPLLDAEITIPKHVELLVEDIFNLPSPHI
                     MPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIGNKLPIVLTGAMRSS
                     NELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTKTHTTNVATFRTPTL
                     GPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGDLLEAIAHTKVDGLV
                     IEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDYEGGGKELEKMGIIF
                     CNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6810..7643
                     /locus_tag="ONT64_002400"
     CDS             6810..7643
                     /locus_tag="ONT64_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR2303793.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7677..8516
                     /locus_tag="ONT64_002401"
     CDS             7677..8516
                     /locus_tag="ONT64_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2303794.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8542..9102
                     /locus_tag="ONT64_002402"
     CDS             8542..9102
                     /locus_tag="ONT64_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR2303795.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9301..11022
                     /locus_tag="ONT64_002403"
     CDS             9301..11022
                     /locus_tag="ONT64_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCR2303796.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
BASE COUNT         3762 a         1816 c         2215 g         3238 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt tttttcattt tacagacttc cttacgaata
     6601 attttgcata gccgttaaat cttattgtat actatataaa agagttttgc tatcagtgaa
     6661 gaataacgaa tagcctaact tatcataaaa aagctgacct tattaaaatg gaaaagactc
     6721 aaccttgacc tcctaataca atggagtatc tggttgagtt tcttttacaa gaaagcagcc
     6781 aaggaaatca atcacgaagg tggccggaaa tgatcagaat cggattaaca tcgtttaaag
     6841 aacatgacaa attaactggt aaaaagaata acactcttta tgaatatgca gggtttctac
     6901 ccttagtgga attagatact gcctattatg gaattccccg aaagtcctca gttgagaatt
     6961 ggataagtga agtacctgaa tcatttcgct ttgtagtcaa agtctatcaa ggaatcagcg
     7021 gtcaaggaga ctggaagcct tattatcaga ccgaagaaga gatgattgcg cattttttag
     7081 atgttatgtc gccaatggta gatgctggaa agatgttttg ctttttagtc caattcccct
     7141 catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg caaacttaga aagtggtttg
     7201 agggttatcc tgtttctata gaattgcggg attattcttg gtacagcgat gaatttcttg
     7261 aacgtacaag aaactttatg tctagagccg ctttttcttt agccatcatc gatgagccac
     7321 agttgctgaa tacaacgatt ccttttgatc catttgtcac caatccgaac tttgctcttt
     7381 ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa tgataaagag tggcgtaaaa
     7441 aaagaacgtt atatcgttat tcagaaaaag aactgcaaga actagcacat gaagtcaaag
     7501 tagttgattc taagacaaaa gaggtaggtg tgatttttaa taataattct ggtggtgatg
     7561 cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat caagccagat ggactcaatc
     7621 caacccaaat ggatctgttt taagggctta ttttattcaa gaaaggggcg aagtctatga
     7681 tcaaagctat tttttttgat attgacggaa cattggtcac caatcgttca aaagcgttgg
     7741 aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg cattttatgc ggtgtagcaa
     7801 cggggcgcag cccagtaaag atcaaagaaa tcattgatga attggaatta gatatgtatg
     7861 tcgtgtataa tggtcaactg gtttttactg cagatcgcac gattatcgac cacccttttg
     7921 aacaaaaagt attggaacat attgttgaat ttgcagatga gaaccatcga caaatcgttt
     7981 ttggtgcgag aaatcggtta gatggatcaa cgacgatgct attaggacaa tcgattttta
     8041 tcaagcgact agtcagcttt ttaccgcgta aattcccggt acgtctgatg aaaaaaatcc
     8101 tccaggtttt tagccctcac cgacaaaagg atcgttatga aacgttagag atattaaaag
     8161 aaccaattta tcaatgtata ttgctcagtc cggagtcaga acagaaaaag ttagaacatc
     8221 gctttcccga gtgtacgttt cagcgttcaa atacgtatac tgtagatatc atcccaaaag
     8281 gtggttcaaa attactaggc atccaagctt tcgcaaatgc tgtcgaaatt gaaatggaag
     8341 aaattatggc attcggcgat cattacaatg atatcgagat gctgaaaggt gtaggaattg
     8401 gagtggccat ggggaatgct cagatcgaag taaaacatgc tgcagacttt gtgactcaat
     8461 caaatgaaga agatgggata tacgaaggac tgaaacactt cggtctgatt tattagtcaa
     8521 tgaaagaagg aaaaaatcag tatggaagca gagacacctt ttcaaaaagt agaaacattc
     8581 cacaaaactt ttgatccaag aagaccagag atacccacgg cttttacaga aaatgaagct
     8641 catacacgtg ctgcgttcaa aatagaagaa cttgttgaat ttgttgcagc tacttcacct
     8701 aataaagaac aactgcatga agctgttatg ctgctgcacg aagcattaga taaagcagaa
     8761 gaaaaaatac ttggtaaaac acagtgggga agtgatccgc tggttgaaca agtagatgca
     8821 ctgacagata tcctttactt tacttatggt tctttttcat tactaggaat agtgcctgat
     8881 cgaatattcg aaatcgtcca tcaggcaaat atggggaaat tatttccaga tggaaaacct
     8941 cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa actggcagat ggaatatgct
     9001 ccagaaccta aaatcgcggc agaaatccaa cgtcagattc aagaagcgat tgctaagaag
     9061 aaatccgctg atataggcat gtgcacaaat gaaaaagagt gatcagagtt tcactatttt
     9121 tgttatcttt atacgatgga aaaacaaaag attttcgttt aagttagtga atgcatgaaa
     9181 atagtatgct ataatctaga atagatgtct tttgcacgag agacgtatta tttggattta
     9241 tgccaagcag aacgccatct gttgcataaa agatgcatac tgaaaacgga ggaaccataa
     9301 atgggaaata atgttatcat cggaatcgtc attgcaatca ttatagtcgc cattgtcgtc
     9361 tttttggtta gttacttgat gaagaaaaaa aaccaagatc gattggatgc cttagaaaaa
     9421 cgcaaggaag aattatttga cttgccagtg attgaagaag tcgatgatgt taaaaaaatg
     9481 catctggttg ggcaaagcca aaataccttt cgtgaatgga gtcaaaaatg gaatgacata
     9541 tctacttcct cgttcgctga actagaaagt caaattttcg aagtggaaaa cctcaatgaa
     9601 agttttcggt tcttaaaagg aaaaaaagca gttgttgaag cagaagctac aatggaccaa
     9661 atggagaaac aagtgacgga aatccgtgaa ggtctaaagg aattgcggga aacggaagaa
     9721 cgcaactcac tagctgttca gcaggcactt gacgtctatg aagaactgaa agaaaaagtc
     9781 agagaagacg agcatgaata cggaccagct ctttttgaat tgcaaaaaca gatcaaagtg
     9841 atcgaaaaag aatttacgca gtttgtgact ttaaacactt ctggagaccc tgttgaagcc
     9901 cgcgaggttt tggataaagc agaaaaacat acgtatgaag tggaagctct gatgaaaaaa
     9961 attccaccgc tgtatgagga gctccatacc actttccctg aacaacttga agaaatcagt
    10021 gatacttacg agaaattgat ggaagaacaa tatgtattcc cagaagaaga tcttgctgaa
    10081 gatatcgcaa aagtaagtcg aagaattgaa aattcattag ccaatcttga aaaaacagaa
    10141 gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt tgattgattc gttatatgat
    10201 atcttagaac gggaaatgga agctcagaga tatgtaaaaa ctaaccaatc aacgattgca
    10261 gagtatatca aacatacaac aaagaataac cgtcaattat tgattgagct ggatcacaca
    10321 tctcaaagct atacattgaa ccacaatgaa attggccgtg tacgaggttt ccaaaccgaa
    10381 gtggaagaaa tggaacgtca aaatgaacag atgattccac agatccgtca acacgaaatt
    10441 ccttattcag aagtgcgcac gttctataaa acagtcttca aagtcttaga agatatcgaa
    10501 aagcaacaag tagagatcga cgactcatta catgaattga gaaaaggcga aaaagaagcg
    10561 caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt tgaaacgttt tgtagaaaaa
    10621 caacgtttgc ctggcttgcc gaatgattac ctagaattct tttttgttgc tactgaccgt
    10681 attgaagaac taagtgttgt cttgaataaa attcgagtaa acatggaaga ggtcaatcgt
    10741 ttagttgctc tatgtgaaga agatctagaa ttattagata agaagacaca tgatttagta
    10801 gatgcagcag cattaacgga gcaaatgctc caatatgcaa atcgttatcg tcatacacac
    10861 gaagaagtga gagaagcaat tgataaaagc tactatttat tcaataaaga atatcactat
    10921 caagaagcat tagacgaaat cggtacagca ttagagcgag tagaacctgg tgctttcaaa
    10981 cgcatcgaag atttttattt taatcatcca gacttagtat aaagcaaaaa a
//
LOCUS       DAJSAW010000074        10929 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJSAW010000074 DAJSAW010000000
VERSION     DAJSAW010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.074"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..288)
                     /locus_tag="ONT64_002404"
     CDS             complement(<1..288)
                     /locus_tag="ONT64_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2303797.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAEN"
     gene            430..1548
                     /locus_tag="ONT64_002405"
     CDS             430..1548
                     /locus_tag="ONT64_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR2303798.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1676..3052
                     /locus_tag="ONT64_002406"
     CDS             1676..3052
                     /locus_tag="ONT64_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR2303799.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3453..5033
                     /locus_tag="ONT64_002407"
     CDS             3453..5033
                     /locus_tag="ONT64_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR2303800.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5231..5454
                     /locus_tag="ONT64_002408"
                     /pseudo
     CDS             5231..5454
                     /locus_tag="ONT64_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5659..5979)
                     /locus_tag="ONT64_002409"
     CDS             complement(5659..5979)
                     /locus_tag="ONT64_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCR2303801.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6127..8361)
                     /locus_tag="ONT64_002410"
     CDS             complement(6127..8361)
                     /locus_tag="ONT64_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2303802.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8840..9106
                     /locus_tag="ONT64_002411"
     CDS             8840..9106
                     /locus_tag="ONT64_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCR2303803.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9106..10833
                     /gene="ptsP"
                     /locus_tag="ONT64_002412"
     CDS             9106..10833
                     /gene="ptsP"
                     /locus_tag="ONT64_002412"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR2303804.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1912 c         2216 g         3322 t
ORIGIN      
        1 gttttctgct tcttcaatca atatttttcc taacccttgg tgtctgtatt cagaaagaat
       61 cgccatccgc tgtaatttca tttttccgtt ttctaaaggc aaaagacgta cagttccttg
      121 cggttctttt ttatctgagt acaggacgaa gtggatacaa tgggcttcat ctttgtctat
      181 ttctcgagac aaaggcacac cctgttcgac tacaaaaact tgatgcctga tccgtacagc
      241 atctaagtaa atattgctca ttgtatcttt tgtttgtaca actttcatgg tctagccacc
      301 ttcctttcgt atagtatact ggaaaataaa acaaataaaa agtatggcaa gttttcaaaa
      361 ataaaaaaag aataaaagaa tttttaacga atataatata tactagtaag aaagaaagga
      421 ggaaatctat tgtttgaaaa acttagacag tctaaactaa tgttttggtc gcttgaatta
      481 ttgatcatag ctgcattgat catgatttca tcagaaataa actttatttt cgaaccgatc
      541 gggacatttt tttccacgtt atttgcacct gtactgattg caggctttct ttactattta
      601 ttgaatccta ttgtcaagct gttgatgaaa gcaaaaatca aaagaatatg ggctgtcgca
      661 attgtgctgc tccttttagt tgcagcaatt atttggattt tacttagtgt catcccaagt
      721 ctagtacagc aaatttcctc attggcatct aatatgcctg atttcatcaa acaggtagaa
      781 agctggttaa aagaggtagc cgaattgcct ttgttcaagg aagtagatat caataaatat
      841 tttgagcaat tagacatttc ttacgggaca atcattcagc aattccttag tggagtatcc
      901 aatagtttag gttcgatcgt tggaacgatc gcttcggcaa ccgtagtaat cattacagca
      961 ccattcatct tgttctatat gttgaaagac ggagaaaaac tagtgccaaa catccaacag
     1021 tttttcccag aaaaaagaag aaaccaaatt gtggagttat tggggcaact gaataaaaca
     1081 ttggctaatt acattagcgg acaagcaatc gaatgtttat tcgttggtac cttcacattt
     1141 ttaggatata tggtaattgg tgtggattat gctttcttat ttggggtcat tgcgggattg
     1201 acaaacctaa ttccatattt aggaccttat ctaggactag cacctgcatt tctagtaaca
     1261 gtcttcaatg atccggtaaa agcattactt tgttgtgtgg ttgtgctaat cgtccaacag
     1321 ttggatggga atatcattta tcctaatgta attgggaaat cactgaaaat ccatccatta
     1381 acgatcatta tcgttcttct agtagctgga aatattgccg gattgcttgg tatcttctta
     1441 ggtgttcctt tttacgcaat ctgcaagacg atcatttcct atataatcaa aatcgtcaaa
     1501 gaagacaagc aaaatgaaaa caagaagaaa attgctacga cctcgtaggt agttttagta
     1561 cacttgtttt tattgcaaag aaaattgctt tgtgatacca ttttaacgtg gctatagtgc
     1621 cacgtttttt tctatcgtaa actaaaaatt aattttataa ggagagaatg cgattatgaa
     1681 tgctgaccct gagagtcagt cgctgttagc gcagatttta ttattgatcg ttttgacatt
     1741 gatcaatgct tttttagcag cttccgaaat tgctgttgtt tcgatcaata agaatcgaat
     1801 cgagcaaaaa gctgatgaag gagatgtgaa atctcaaaag cttttaaaaa tattgcagaa
     1861 tccaaataat ttcctatcga caatccaagt aggaatcact ttggtcaaca ttttgtctgg
     1921 tgcttcacta gctaatactt tatcggaacg gttagctccg gtattaggtg gcggcacagc
     1981 ggcaaaaaat attgcaaata tcattgtgct ggctattttg acctatgtat cgattgtttt
     2041 tggagaattg taccctaaac gtattgcttt aaataaatca gaagaagtcg caaagttcac
     2101 ttcagggctg atacgcttga ttggtgtagt agctaagccc ttcgtctggc tgctttctgc
     2161 ttccacaagt ttattggcgc gtatcacgcc gatgactttt gatgatgaag attcaaaaat
     2221 gaccagagat gaaatgcgtt atatgcttga aaacgaaggc gtattgaata atgaagaatt
     2281 agaaatgctc caaggggttt tttctcttga tacgaaagtt gcccgagaag tcatggtccc
     2341 acgaacggat gcatttatga ttgatatcaa cgacactgtg gaagaaaatg tgaatgaagt
     2401 tttatccgaa aactattcaa gaatccctgt ttataacgaa gacaaagata aagtagtcgg
     2461 aatccttcat acaaagaatt tactaaaagc tgcccataaa ttcggctttg ataatttaga
     2521 tatcaagaaa atcatgcagg agcctttatt cgttcctgaa acggtcttta ttgatgatct
     2581 gttatatgaa atgaaaaaga cgcagaatca gatggctatt ttattagatg aatatggcgg
     2641 cgtcgttggg ttagcaacac tagaagacct tttagaagaa atcgtcggag aaatcgatga
     2701 tgaatcagat gaagttgaga atctttatga aaaaatcgat gaacacgaat atatcatcca
     2761 aggacgtatg ctgattgatg agttcaatga agcttttgac agtgatctgc atatgagtga
     2821 tgtagatacg atggctggtt atttgattac agcattaggc atgattccgg atgaaggcga
     2881 aaagttatcg tttgatgtcg ataatattac gctggtttct gaagaaatgg aaggttctcg
     2941 aatccttaaa atccgtgtca tttttcatga tccggaagaa acagaagcgg aacctgaaga
     3001 agaacgaaga tacttcagaa aagaattcga agatgacgag ccaagaagat aattggaaac
     3061 ttggttatga aagacgtgtt gcttgaataa acaagcaaca cgtcttttct tttagtcagc
     3121 tgacaaaaaa atatttgact cttatggttt ggtggctttt tcattcgctg aatccatgct
     3181 atactgattc agttgtagta gttgacaaag gttgtgaaaa agctatcctg cattaagtaa
     3241 taagagcagc caagcaaaaa cagcttttcg tatttttggc ttgggtgggc ttattgctga
     3301 taggctgtct ctagaacgcc gtttactcgg aaaaagagac tgctacacgg cttttgtcac
     3361 agccctatat gaaattgtct ttctttaaga gatcggacaa tctgtgccta gagaaaatat
     3421 aaataattga atggaagaaa tggagttagg ttatgaataa tccaaaatta aagcaacaag
     3481 tggacaatcg cagaactttt gcgatcattt cccatccgga tgctgggaaa acaacgatta
     3541 cagaacaatt actattgttt ggtggggcta tccgtcaagc cgggaccgta aaaggcaaaa
     3601 aaacaggtaa ttttgccaaa tcagactgga tggaaatcga gaaacaacgt gggatttctg
     3661 ttactagttc tgttatgcag tttgattatc aagggaaacg agtaaatatc ttggatacac
     3721 caggacatga agatttttct gaagatactt atcgtacttt gatggccgtg gatagtgcgg
     3781 tcatggtaat cgatagtgca aaagggatcg aagcacagac aaagaaatta ttccaagttg
     3841 taaaaaaacg tggcatcccg atatttactt ttatcaacaa actagatcgt gatggtcgtg
     3901 aaccattgga attgttagaa gaattagaag agctattaga tatcgaatct tatccgatga
     3961 attggccaat cggtatgggt aaagggctag aaggtcttta tgatatctac cataatcgtg
     4021 tagagtttta tcgtccggaa aattatcagg atgaacgatt agtagaattg gatgaagatg
     4081 gactgcttcc cgaaaatcat cctctaactg aaaattcctt atatgaacaa gtcttagaag
     4141 aagtagaact aatcaaagag gctggagatg catttaatca agaaaaaatt gctcgtggcg
     4201 atcagacacc agtatttttt ggttctgcat tgactaactt cggtgtacag acttttttag
     4261 agactttcgt tgaattggca ccagctcctt atggacacaa aacacaagat gatcagatgg
     4321 taagtcctta tgaggaagaa ttttcaggtt tcgtattcaa gatccaagca aatatgaatc
     4381 cagcccatcg tgaccgaatc gcttttgtcc gaatctgttc tggaacattc gaacgaggaa
     4441 tggatgtctg gttggaacgt acaaataaaa aactgaaatt aagcaatgtc acacaattta
     4501 tggcagattc gagagaaaac gtcgaaaaag cagtagcagg cgatatcatc ggtgtatatg
     4561 atacaggcaa ttatcagatt ggggatacat tgttcgaagg aaaactaaaa gttgcttatg
     4621 aagaactccc ttcatttact ccagagctgt ttatgaaagt gactgcaaaa aatgtcatga
     4681 aacaaaaatc cttccacaaa ggaatctatc aattagttca agaaggagcg attcagcttt
     4741 ataaaacata tctaacggaa gaatatatca ttggagctgt tggtcagcta caattcgaag
     4801 tattccaata tcgtatgtta aatgagtata acgccgaagt aatcatgtct ccaatgggac
     4861 acaaaatcgc tcgctggatc gatccagaag atttggatga gaagatgagt tcaagtcgca
     4921 atatcttggc tcgtgaccgt ttcgaccaac cgttgttctt attcgagaac caatttgcag
     4981 aacgctggtt tgcggataaa tatcccaatg taaaattaaa aagtttgatg taaagaagtc
     5041 ttgacacaag ctgccctgca tcaagtaata agaatagcca aacaaaaaca gcctctcata
     5101 ttttgtttag agccgtgacg gattttccgt gacggctctt tttactatat taaaatcata
     5161 gaaatgtcta atcaaaaaaa tataaaaatt attttgcgta catatgatta aagaaaggag
     5221 agtgtgacac atggaaacag aatggcaaag acgtgaaaga aaaaaacaga aaaagaagag
     5281 atgttacgat tatgtcgatt attagatgta aaagactggg acaaaaacga gcaaattgca
     5341 caaaaagtac gatcaatcat ccataaagag gaaaaagtta aaaataaaga cacgcgtaga
     5401 actgaaaatg aaaagagaaa agtacaaaag aaaagagaaa aatcaaaaag ctagaaagaa
     5461 aaagaggctg agacagaagc gtttaactcc aagaaataag ctggaattca caaaaatttg
     5521 aaaagcaatt ttcgtgaatt tcagcttatt tccgaaggag ttgcttctgc tttcgccgtt
     5581 tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg ctctttaatt
     5641 attatgttca aaatttgttt acacggctaa tgtagcgcct aattcatgtt ttggaatcga
     5701 gatttcaaca aggcctgcca attcgattac tttgatttca tcctcgtggt aaacggcgtc
     5761 tttgatttta tctaagaaat aatttttagc atcttcgatt tctttcggct ggattttacg
     5821 tttgtcattg atcagcagaa tttcattatg ttcaggggct tcagtgtaga caatagtggt
     5881 atttccggct gtgtagacct tgactagatg ggcatcagtg ttttcaagct ggtttaatac
     5941 aagacgggaa tggctgtttg ttacatttac caatttcatg gcgctcacct cttttatgta
     6001 tttttaattg cttgtttctc ttattataat ttctttttta taaaatgaac agtattttga
     6061 cttttctgaa aaaagaatga gacggcattt ctcaaagaaa taccgtctca cgctataaaa
     6121 ctggatttat tcagccgttt cttcggctga ttctttagcc aaacgttctg gtttagaagt
     6181 aacgatgatt tttccatctt tatccagttt tgctttcaac tcatggatag aagggtgatc
     6241 aagataaaat tcagcgatac catcttcgat ttgttcttgg atcgtgcgtc gaagaggacg
     6301 agcacccata gatggatcat agcctaagtc aactaatttt tctttgacat tcgtaggaac
     6361 ttcgatgtga agttgctgtg ctgccagcat ttggttgaca tcgtctagca ttagtgtgac
     6421 gattgtcatc aagttttctt ttgataatgc actgaattcg atgatcccgt caaaacggtt
     6481 cagaaattct ggtgtaaagt agttgtttaa ttggttcaat acagaacgag taacaccttc
     6541 gcgtgccgca ccaaaaccaa cgtttgcttc tactttacca gttccggcat tacttgtcat
     6601 aatgatgatc gtatctttaa agctgaccgt tcttccttgt gcatctgtca atcgaccatc
     6661 atctaagatt tgcaagaaca tatgcaaaac atctggatga gctttttcta cttcatctaa
     6721 caagacaaga ctgtaaggat ttctccgtac tttttctgtt aattgaccag cttcttcgta
     6781 tccgacataa cctggaggag aaccaatcaa tttagagaca ctatgttttt ccatatattc
     6841 agacatatcg aaacgaatca ttgaatcttg tgaaccgaat agttcgtagg caagttgttt
     6901 cgcaagctct gttttaccta caccagttgg tccaacaaat aagaaggagc cgattggacg
     6961 attctgtttg tttaatccaa cacggtttcg gcgaatcgct ttcgctactc gatctacagc
     7021 gttatcttgt ccgatgacat gagctttcaa gtcatctgct aagtttttca gttgtgtctg
     7081 ttctttttct tttaattcac ccactggaat acctgtccgt tgttcaacga ttttttccat
     7141 atctttttcg gtaatcacag gtgtttcctc ttcagtgagc tgacgttctt tcattttttg
     7201 aagtttattg atctgatcgc gataataagc tgctttttcg aaatcttctt ctcgagaagc
     7261 taaaactttt tgttcttctg cttcttgcag ttttttctcg attgttttag gatcaacgat
     7321 ttggatcgtc aagtttttct tagaacctgt ttcatctagc aaatcgatgg ctttatccgg
     7381 caagaagcga tcttggacgt aacgattgga taaagttgcg gctgcttcga tggcctcatc
     7441 agtatatttt acatgatgat agtcttcata acgtttttgc aaacctttca aaatactgat
     7501 cgtttcttcg acagttggtt catctacccg tacaggttgc atacgacgtt ctaaagcagc
     7561 gtctttttcg atgatgcggt actcatttaa ggtagtagca ccaaccattt gcaattctcc
     7621 tcgagctaaa gctggtttta aaatattacc agcatccata tttccatcac cagcagaacc
     7681 agcaccgacg atttcgtgaa cttcatctat gaataggatc acattttctg cttgacggat
     7741 ctcttcaatc aatttttgca tacgttcttc aaattgtcct cgtataccag taccttggac
     7801 taaagatacg acatctaaac gaatgacttc tttgtccatt aatttttgcg gtacatcacc
     7861 atctacgatt ttttgtgcta aaccttcaac aactgctgtt ttaccaacac ctggttctcc
     7921 aatcaagaca ggattgttct tggttcggcg atttaaaatt tcaatgactc gtttgatttc
     7981 gtcatcgcgt ccgatcacag gatcgatatc accttgtcgt gcttgttctg tgatattgat
     8041 tccgtattct cccaataaac cttctgtatt gccttgaggt ggctgaccac cattgaagtt
     8101 gttgtttccg ccaaattgtg ttggtggtgt ttgctcataa tttccttgct gttgcatttg
     8161 gcgagacatg gaacggaaaa gatcgtccaa actgccaaat ccaaatggat cattttgggc
     8221 catattagtc agacctccat tttgttgatt tttgattttt tgataacagc tctgacagta
     8281 atcgagttgt gtccgctgac cattgacagt ggcatacaag tgaatcgttg cctcattctt
     8341 gccgcagttt tgacaaagca tgaatcattc acgtcctttc acaaaaatgg gtacatccat
     8401 attttacatc tcgtttttca tgaggtaaaa gaataacccc tagtttttta agagatttat
     8461 gaggtagaaa aggtgaaatc tctattgacc attcctgacc tttgactctt attatactaa
     8521 aaataaaaag ggaagcaagt aattcgtttc atatgaaaac aaagagggtc aaaggaaaga
     8581 atgataaacg tcaggagaag agaaagcgac aagaaaaagg aaataaacac gtttgttata
     8641 aaatgtgaag aaaaggatga aaaagaatcc ggaataattt gaatactttt atctgaaatt
     8701 gaatgaaaac tgttgtatct aacgaaaaaa aatgttaatc tttacagatg aaagggtggc
     8761 atttgttagg attctctgaa ttactaaaaa agaaaaccct cacataaaaa caacactcac
     8821 ttaaaggaga ccgattaata tggaaaagaa agaatttcac gtagtagcag aaactgggat
     8881 ccacgctcgt ccagctacac tattagtaca aacagcaagc aaatttaact ctgatgtaaa
     8941 cttagagtat aaaggtaaat cagtaaactt gaaatcaatc atgggcgtta tgtctttagg
     9001 tgtaggtcaa ggttctgatg ttactattac tgcagaaggt gctgacgaag ctgatgcaat
     9061 ggcagcaatc gttgaaacaa tgaagaaaga aggcttatct gaataatggt tgaaatgcta
     9121 aaagggattg ccgctagtga cggtgtagcc gttgcaaaag cttacctgct agttcaaccg
     9181 gatttaacat tcagtaaggc aacagtagaa gatactgcgg ctgaagaagc tcgtttggat
     9241 gctgcattag ctaaatctac tgaagaattg cagcaaattc gtgaaaaagc agcgcaaagc
     9301 ttaggtgaag cagaagcgca agtatttgat gcccatttga tggttctttc agatccagaa
     9361 atggtaggtc aaatcaagca aaatattaaa gacaacagcg taaatgctga atcagcgcta
     9421 aaagaagtaa cggatatgta tatcggtatg ttcgaagcaa tggaagataa tgcttatatg
     9481 caagagcgtg ctgcagatat tcgtgacgtt gctaaacgta ttttagcgca cttattaggc
     9541 gttactttac caaatccatc aatgatcaac gaagaagtgg tcgttgttgc acatgacttg
     9601 actccaagtg atacagcaca attagatcgc aattttgtaa aagcgtttgt aacagacatt
     9661 ggtggacgta cttcacattc tgctatcatg gcacgttctt tagaaattcc agcaatcgtt
     9721 gggacaaaag aaattactgc taaagtcaaa gaaggcgata ttttggctgt caacgggatc
     9781 gaaggcgatg taatcatcga tcctacagat gaacaaaaag ctgaatttga aaaagcaggt
     9841 gcagattacg cagcacaaaa agcagaatgg gaaaaattaa aaaatgcaga aactgtcaca
     9901 gctgacggca agcattttga attagctgca aacatcggaa caccaaaaga tctagttggt
     9961 gtccataata atggtggaga agcagttggt ctgtaccgta ctgaattcct gtatatggat
    10021 tctccagact tcccaacaga agatgaccaa tatgaagctt acaaagctgt attagaaggt
    10081 atggaaggaa aacctgtagt tgttcgtaca atggacatcg gtggagataa agaactacca
    10141 tatctgcaat tacctcatga aatgaatcca ttcttaggat atcgtgcatt gcgtatcagt
    10201 ttatctgaac aaggcgacga aatgttccgt acacaaatgc gtgcattgct tcgtgcatct
    10261 gttcatggta atttacgtat catgttccct atggtagcta cactcaaaga attccgcgca
    10321 gcaaaagcga tctttgaaga agaaaaacaa aaattgataa gtgaaggcaa ggaagtatct
    10381 gatacgatcc aagtcggcat catgatcgag attccagcag cagcagtctt agcagacaag
    10441 tttgcaaaag aagtagattt cttctctgta ggtacgaacg atttgattca atacacaatg
    10501 gcagcagacc gtatgaacga acgcgtttct tacttgtacc aaccatataa cccatcaatt
    10561 ttacgtttga tcaaaaatgt aattgatgca gcacacgctg aaggaaaatg ggcaggtatg
    10621 tgtggagaaa tggctggaga tcaaacagct gttccattgc tagtaggtat gggtctagat
    10681 gagttctcaa tgagtgcgac atctattctt aaaacgcgta gtttaatgaa acgcttggac
    10741 acagcgaaaa tggctgaact tgctgatcgt gcattgaaag aatgcgatat gatggaagaa
    10801 gtcgttgaac ttgttcatga atatgtaaaa taaacaacgt atcaaaagct ggtgtggctt
    10861 gtaaaagagc tacgccagtt tttttgttct tttttaagaa aacgtttttg tcagtaagaa
    10921 tagacaaaa
//
LOCUS       DAJSAW010000075        10817 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJSAW010000075 DAJSAW010000000
VERSION     DAJSAW010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.075"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(25..162)
                     /locus_tag="ONT64_002413"
     CDS             complement(25..162)
                     /locus_tag="ONT64_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCR2303805.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="ONT64_002414"
     CDS             complement(159..1343)
                     /locus_tag="ONT64_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2303806.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="ONT64_002415"
     CDS             complement(1599..1853)
                     /locus_tag="ONT64_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303807.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="ONT64_002416"
     CDS             complement(1878..3020)
                     /locus_tag="ONT64_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HCR2303808.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="ONT64_002417"
     CDS             complement(3080..4432)
                     /locus_tag="ONT64_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCR2303809.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="ONT64_002418"
     CDS             complement(4503..4847)
                     /locus_tag="ONT64_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR2303810.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="ONT64_002419"
     CDS             complement(4857..5231)
                     /locus_tag="ONT64_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2303811.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="ONT64_002420"
     CDS             complement(5244..5558)
                     /locus_tag="ONT64_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2303812.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="ONT64_002421"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="ONT64_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="ONT64_002422"
     CDS             complement(8976..9638)
                     /locus_tag="ONT64_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303813.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="ONT64_002423"
     CDS             10255..10485
                     /locus_tag="ONT64_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303814.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            10491..10790
                     /locus_tag="ONT64_002424"
     CDS             10491..10790
                     /locus_tag="ONT64_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303815.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT         3000 a         2387 c         1833 g         3597 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
    10741 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa ataaaaaaat
    10801 aacaaacaaa ctcgctt
//
LOCUS       DAJSAW010000076        10576 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJSAW010000076 DAJSAW010000000
VERSION     DAJSAW010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.076"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            33..656
                     /locus_tag="ONT64_002425"
     CDS             33..656
                     /locus_tag="ONT64_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303816.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /locus_tag="ONT64_002426"
     CDS             1106..2155
                     /locus_tag="ONT64_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2303817.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ONT64_002427"
     CDS             2152..3033
                     /locus_tag="ONT64_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2303818.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ONT64_002428"
     CDS             3036..3806
                     /locus_tag="ONT64_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2303819.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ONT64_002429"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ONT64_002429"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR2303820.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ONT64_002430"
     CDS             5117..6280
                     /locus_tag="ONT64_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR2303821.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ONT64_002431"
     CDS             6397..6807
                     /locus_tag="ONT64_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2303822.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ONT64_002432"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ONT64_002432"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR2303823.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ONT64_002433"
     CDS             9380..9664
                     /locus_tag="ONT64_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303824.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="ONT64_002434"
     CDS             9824..10060
                     /locus_tag="ONT64_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR2303825.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="ONT64_002435"
     CDS             10057..10455
                     /locus_tag="ONT64_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCR2303826.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3691 a         1770 c         2234 g         2881 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattt
//
LOCUS       DAJSAW010000077        10086 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJSAW010000077 DAJSAW010000000
VERSION     DAJSAW010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.077"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(247..759)
                     /locus_tag="ONT64_002436"
     CDS             complement(247..759)
                     /locus_tag="ONT64_002436"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR2303827.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ONT64_002437"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ONT64_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR2303828.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="ONT64_002438"
     CDS             complement(3212..3655)
                     /locus_tag="ONT64_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HCR2303829.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="ONT64_002439"
     CDS             complement(3656..4444)
                     /locus_tag="ONT64_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2303830.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="ONT64_002440"
     CDS             complement(4441..5382)
                     /locus_tag="ONT64_002440"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCR2303831.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="ONT64_002441"
     CDS             complement(5559..6146)
                     /locus_tag="ONT64_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HCR2303832.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="ONT64_002442"
     CDS             complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="ONT64_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCR2303833.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="ONT64_002443"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="ONT64_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR2303834.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="ONT64_002444"
     CDS             complement(9063..9998)
                     /locus_tag="ONT64_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2303835.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DAJSAW010000078         9638 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJSAW010000078 DAJSAW010000000
VERSION     DAJSAW010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.078"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(252..674)
                     /locus_tag="ONT64_002445"
     CDS             complement(252..674)
                     /locus_tag="ONT64_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR2303836.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            complement(664..2838)
                     /locus_tag="ONT64_002446"
     CDS             complement(664..2838)
                     /locus_tag="ONT64_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HCR2303837.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2854..5196)
                     /locus_tag="ONT64_002447"
     CDS             complement(2854..5196)
                     /locus_tag="ONT64_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303838.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(5218..7761)
                     /locus_tag="ONT64_002448"
     CDS             complement(5218..7761)
                     /locus_tag="ONT64_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2303839.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7771..8169)
                     /locus_tag="ONT64_002449"
     CDS             complement(7771..8169)
                     /locus_tag="ONT64_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2303840.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(8250..8534)
                     /locus_tag="ONT64_002450"
     CDS             complement(8250..8534)
                     /locus_tag="ONT64_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303841.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8558..9556)
                     /locus_tag="ONT64_002451"
     CDS             complement(8558..9556)
                     /locus_tag="ONT64_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2303842.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2769 a         2038 c         1495 g         3336 t
ORIGIN      
        1 atactgaaac gtaaaaaaaa taaacggatt tcaaacaaaa tacgtttatt ttttctattg
       61 attttagctc aaaatcgatt caattcgagt gaaatttgct tcaattttca attggttttt
      121 gttcaaattt tgaaacattt ttgctcaaaa aatgagtgaa tttgtttcaa aagaaaaaca
      181 aatacgatcg aaaaaaattt gaactcataa caattttttg tttttttcaa cgtattctct
      241 ttcaattttc actaaaaatt aggaagaaat tgattcaatg tcttttcaaa aaagaatgtt
      301 tttctctcaa tttttttgac gtttctatca tgaatcacga gaaaacttgg gacactttta
      361 acatggtaac gttgtaaaaa agtttgaagt tcttcttgtc ttggttcttt tcttgtttca
      421 attcgatata cattcctaga gaattttttg tcgtatcttt ccaagaaaga ttcaacttct
      481 tggcattttt tacaatcatt tcttgctaga tacacaaaaa atgattgatt cgttttttga
      541 agttctagta cttcttctaa attactattg attagttttc catttgattc ttcgttagtt
      601 tttatcagtt taaaacaacc aaataagagg aaactaacaa taagtatcct aacaataact
      661 ttcttatttc gcatattgtt tctccaaatt tggtttccga atatagacga ctcgcggtgg
      721 ttcctggacg tcttcagctg ttcgatacac cacaaattcg cctgttctca atgctcgaat
      781 gcgattcgga ttgaatttaa agcggtctac gtctcgtttc gtgcctcttt ctcctgtcca
      841 atcaaccttt cccatatcag aataacctgc ttcttgtgtc gtcatttccg ttaattcaat
      901 gtcttcttct gtccccatta aatctgccca ataatcaatt tctgtcgggt catttgtctt
      961 cccaataaag aacgtattca cattcccaat caactgtttt cgaaggacag gatcaattct
     1021 atcaatgtcc gcaagcgttt gaggggcata cgccgcatgc accccagcgc ctcgtccctt
     1081 attgactaag tctataaggt tatcatttag ataagaagct ggttcgtcaa atattactag
     1141 gactttcgag cgattggctg tttctcctgc ttgtttatac acttctgacg caaagaaatt
     1201 aatatctccc acaagcattt gagccaatgt ccgaataaac tcactataaa tcaatccgtt
     1261 taacgagata taaacaatct ttccttcttt aacaacttct tgaatatcta attctctttt
     1321 tcctccagaa gtatcaaaga gttcaccaag ctcactatcc aataacatat tgatattggt
     1381 ctgtaaattc tcagaagtcg catacaaata aaacgggttc ttcttatgtt cgtaacgaac
     1441 aaacaaccgt ttaaacaacg tttgctcgat ttctgtcatg agagaaatgt tttcttttaa
     1501 taatagataa tacgtttcta agtcaacggt tttacgatca tgttcaacga ttcgctcttc
     1561 acttaactgt tccacatcgg caaaatcacg ttctgttttg tccatcgctt cattcttttt
     1621 cgtttttttc ttcgtggttt tcttcttttc tacgaccact ttttctttaa ataaatctgg
     1681 atttttcaac tgaatacgat ccgcaaacaa atgtaagacg tttcgtggca ataaataatg
     1741 ttgtaaccaa actagatttc tttcaatttt atggctcttt tggaactcgt ctaaaagctg
     1801 aatcgttacc tgtaataacg ctttggctgc tgtcgaataa tacacgcttt ctgtttctgc
     1861 tagattggtt aatttatctc gaatggcaat atcattgcca ttttttacag gattataacg
     1921 caaatcgcca ccgtccacaa agacttcaat cgggcgattg tatgcctctg ccacttcttt
     1981 taacgcttgt aaagtctctc ctgctccttt cccatcgact aagataacgg ggatacgaaa
     2041 cttacacgca tgttctgcta gtaattggat caccgtcgtt tttcctgaac ctgtagtcgc
     2101 tggaataatc acatgttggt tcaattcact aaagttcatc acataaggga gcccatactc
     2161 atctgttcct aatagaatca ctttggctaa tgaagccagt tgtttattcc gcaaagcaac
     2221 gtcttttatg acaattgcta cctggtacgc ttgctgatac tctgctgtat atttttggga
     2281 ctcctgaaat ttattcttcc gacgttcctt gtattcttta ctatttagca aagctgtttg
     2341 ccgttctttc tttgaaacaa cttttctttt ttcaataaaa gacgcatacg caatcaaaca
     2401 acttcctgca cctgtcccac ctaaaaggag ggcaaaaatc gtttgtccat taaactgcaa
     2461 cgcatgagaa aacaagttta atggaaataa taagtaacac gacaaatacc ctctaggaag
     2521 aacaaaaata aaacaaaata gatttaatag cgtaaaacct aacgcaaaaa taaaaaaaag
     2581 agagcttctt cgttttaaga agctctcata ctcaaagtca taaatatgtg cacgtgtctc
     2641 ttttttatct atcataaaga caatcgctaa aggaatactg cctacaataa aaaatggata
     2701 tagacaaaca cctaataaag tgacgataaa ataaagttgt tgaaaccgac tttttggcgt
     2761 ttctttttct tgttttcttt tgcgatcgac agaaaacatt tgctcaaaat gtgtcggtcg
     2821 ttttggtgca ttcatcatac tatcgatttc ctcctatcgt tttcgttgtt tcatttctct
     2881 tcgataaatc ggttgttttt ctcgttttcg tttcattttt gcttgagttg ctcgaaactc
     2941 actccgtgtc ttcggtgctt gttttggtga ttttgtttgt ttcatggttg agtatgcttt
     3001 agatacggtt gaaggcgctt tggtttgtgc tgttactcga ttgactgctg acatatgttc
     3061 ccgaaccccc tctggacgtc gaacagattt cggtgtggta gaacctgttg ctcgtggtac
     3121 attccctttt cttagtggtt tttgtaccgc atgtttttgt cctgcaactg tccgggtttt
     3181 catcgaactt gtttttgtta cgggagtaag tgcctccttc cgacgacgac ttgtttgtcc
     3241 ctgacgacgt tttcgatcgt tcaattctgt tctaggcgta tgcttctggc cataagactt
     3301 gtgtttttgc tttggattcg ctaatgacac caccattgca gaaccctgat tgcgatacgg
     3361 actacgagac gcaccttttc tacttctttc tcttagacta ggattgcctt gcaattctcg
     3421 ttgtaataaa tcggctgttt ttcttgcctg tttctccatt gctgttcgtt gcatacgttc
     3481 ttgactttgc tcttctgctt gtttcgcttg atcatgcaag tctcttgctt tttgtgccat
     3541 acgttccata cgatttggtg ctttatgacg aggttgttgt gcggattcac gccgaaaagc
     3601 acttcgtgtg gcttgtttcg ccgtttgtgg catacgaacg tctgagccac ccccacgttt
     3661 tcgttgacgt tcttgtctct tccgtgcgtt ttctttttct ttttgtttct gtgcttttct
     3721 cgcttctttg cgttctttca accgttgttt ggcttccctt ctcaattttg cttctgcccc
     3781 aaatggattt cccgcaaaag ctaacgctcc tcgaacaccc attcttccag taaatagact
     3841 gcctaaaaag cctcggaaca aataaattcc caacggcgca aacaaataaa caatgatcaa
     3901 gagaattttt tcccatggat cgtcttgtgt actacttaat tggaacccgt aactaagaaa
     3961 agaagaaaaa aggatacatg caaaacctgt cattcctttt aaaaacgtca aaataccaaa
     4021 aactttggca tagttaccaa acacattcat tgtgacaaga aacaatccta aaaaaagtaa
     4081 gaacggcaac atcggtacta acaatagttg catagttcca ataatcaatc gaaacatact
     4141 taagacaaaa taaacaacgg tttgaatcag attcacgaca aaataaaaca aggcaaatgc
     4201 tgtatttttc caactattgt aatattgtat gttcttgttt ttcagtgtat ctgcttcata
     4261 ctctgtgact ttgtcatgaa ttttctcccc tatttttgta cctaaatcat tgtccaataa
     4321 aagattgatt cgatcatatt cttttccagc atatttgact ttcttctcac gaattgtgtc
     4381 tacattggtt gtgccataat ttaacaacag atatggttca taaacattac tataaaagac
     4441 ccgagccgcc atcaatttgc ctgccgtttc cactttttta tccgctgtta cctcttctgt
     4501 ttcttttgtt tcataatttg tttcttcttc aggtgttaac actggattga ccttcacgaa
     4561 cgccgtttcc actgtagaat ctaaagaaaa aagaacatca aaaagattct tatttgtccc
     4621 agaatctttt aatacgctta acccagtaaa aataaggatc gtcataagaa agacacggag
     4681 tgcttgtttc attcgagcct ctccagcata tttgattgcc atcataattg ccgaaaaact
     4741 gattccaata gttcctgcaa tacttaacat attgtccgca attttttgtg ttaagttttg
     4801 aatgggttct cgaatggcgg aaataatatc taattcaaag agaaattcaa ccattgaagc
     4861 attgaattct cctaacattt ttacgccact ccaaacaaaa tttttcatca ctcctgcaac
     4921 attgtacgat agcttttttt taaggttcat tgactcatag tcattggtca tcaattcaaa
     4981 cgaattcttt ttgtaatttt tatacaattg atccacatca gtcgttactt cttttacttc
     5041 tactttatcg ttggcattct ctccttcaga acgatcggtt tcagtgacac ccgcaccatc
     5101 attcatttga tcggcagaaa cagtagcccc ttgtagacag aaaaaaagac cccctaataa
     5161 aaggagcgtc caccatttca gagatttttt tcgcaaaaaa accacctctt ttttgtttta
     5221 tcgttgtttt tcattttcta acattgcttg aattttttca tcttgatttt cagtagaaga
     5281 aacagccaat aaccaactat caaacaacac atcaatggca atcggttgat ttcttcccaa
     5341 atgatcctgg aataaacagg ttcctgtttg taattgttcc atcatttcta aattggctga
     5401 attcacttcc atatcaaaga aggacaatag tttctctcgt gcttcttttt gtttcggacg
     5461 aaacgcaaat ttgtaagaaa ttaactcttt tttatcttct tgatcgtaat ctttaaacgc
     5521 ttgcacaaca aggtaaatat ccgtattatt cgcacgtcct tttcgcaagc tatcatcaat
     5581 caaatagctt ccttgtgcgg tatcttcata cccttttgcc tcgtcataaa taatcgcagc
     5641 gtcttcttct ttgtttgtag aaaagatata cgtaaacttc ataatgactt ccataattgc
     5701 cattcctgca agttgttcgc tatttaatcg acccgaatca atctcttttt gtgtcggaat
     5761 gcgtattcct tgtgtcccta acacgttgat ttgtgaatca aaacgtatcg gcgtactgtg
     5821 ctcattccca atgagaatct ttcctaaacc aacgctatgc cctaaaatca atttagctag
     5881 ttctttgtca ctttttcgaa tttcttcgat aacttgactt aacgaatgct tacctggcat
     5941 ggtgtaaact acatcgatgg cgtcttggat caccgttttc ttgtggctcg ctgtttctcg
     6001 attggtatct acttctccta acattcctaa cacattttta gcggtttgaa ttgcctcttc
     6061 ttttggtaaa aacaatagag gatcaagcat ccctcgacag ctttcatctg aagataacga
     6121 aataaaatta attcgttcat acatagccac aaactctggt atgtgtccga atttttcaag
     6181 tgcctttaaa aagaactttt gggtttcatt tttcggatca atataaagaa ttttttgacc
     6241 taaaaacatc agccataaaa aaatatattt tacaaggacc gattttcctt tacctggtgg
     6301 accaataatc aacgtatttc cattggtatg ttccgcgcct tcaatacttt tctttgttaa
     6361 attgggataa taccatacta gtttactaga tagcgctaac gcttctttcg cagatttaaa
     6421 cttttttgca ataacaatcc gtcctaaagg aaatcctttc ttatttccta cttctttctt
     6481 taagtccaag ccataatctg ccacaaaacc tgtcgtagcg acctgctcgt aactacgaaa
     6541 acgttgcgta cttccaatta agcattgatt aaacaacgtt agctgatccg ctaccgatcg
     6601 ataaaatttg aaatctgtag actcaatcgc aaattcaata tctttgactc gttcttccaa
     6661 ttcttctttt gaagaagcag atacaaccag cgtgacggta gaataacaca atcgtttttc
     6721 tttgcgcttc agcttatctt ttacatccat taaccgttct gtaccaaaat ttaatacatc
     6781 gtctgaatct aaaatgccgt caacggtctc atattctttt tcttgttcat aaatccgtcc
     6841 agccatttta ttaatttttc gttcatcaga ttttgaatgc tcaaatcgaa gtcgaatatg
     6901 cgtatcaatt ggaaaagata agctatcctg aagattttga ataaagctac tcccatacat
     6961 ggctaaagga gcgtccgttt gacttaaaaa cgtcgaataa tgcgtttgat tcaactgttc
     7021 aatcgcaatg tatcctgctt cattcgaaat gacgccctcg caaatttcca ttggcgtaag
     7081 ctgtctttca ggcaattttt cttctgatcg atggaaaaaa taataataaa tttgtccaat
     7141 ctcttgggca gataagcgct ctatcggctt ataattggac aattccgtat agagctcctt
     7201 ttctaatgtt tcatacgttg gtaacacact acttatagga actcgttgtc ccgtaatacg
     7261 atagataaac tttttaaaaa catgcttaag caattgaaaa tattcgatcg gatcagacac
     7321 tttcatttct gtagtgaaac gcacaaacag atacgttcga tactcgttta attgcacttc
     7381 gtcttttaaa atctttcctg cctgttcaaa gtacactttc ccaagatctg cgcattctcc
     7441 ctttaccaaa tctcgaatgg tttcttcaat gtgcgtatct aggttaaatt gacgtggaat
     7501 attgataaaa tgatagtcat attcatcatg tccaagtatg ccttcgccat cttgaagata
     7561 gttcttgaaa aaatcaatgt catttaaagg aaatacttgg gaagaaagtt gatagcctac
     7621 ccacacatca tcatttttgg tcaaaacaaa atttcggata atggcgtcta tcggaaattc
     7681 taatcgagga agcttcgttt ttttcttttt gaactgccaa atcccgataa taaagacgac
     7741 caaaagaaac aaaaacgcca tttactcgac tcactccttc catttgtgta gggaatacgt
     7801 cactttttca tggaaaaacg gcacatacat ttcatgttca aagcgttgtt tccgtttctg
     7861 ataataggac gttcgcccaa ttaaatactc gaaaaagtgt ttattgtccc atttcattga
     7921 aaacaatgtc caaacgagaa caccaatccc aaaaatcata atcaaccagg actttcgaaa
     7981 cagtgtctgc aagaaactaa tttgattcac atacgcaatc aaaaatagga tcaaaagaat
     8041 gaagagaaat accccaccta tgactaattg ttccatgcga atgccattag aaatccaggg
     8101 aattttttgt cctttaatcc cgtaaatttt gtgagggtgc ttaaactcct ttgtatagtt
     8161 gtattccatg ttcactctac ctccttttac aaaaaaagaa gcgaccagaa gttctaatct
     8221 tctgatcgct tttgcagttt gtctttttct tatgataatt gtatccaggg cacaatcatt
     8281 tgcgttaccc ccacgttctc ttcactatcc ttaataaaga taatgttctt tagccattca
     8341 ccaatatcaa cggcattcga taacaataag acgagcatta tcaaaataaa gacgccaata
     8401 atggcaaacg ctgcccgcgc aaagccttgg gtcgcataag caccaaccat tagaacaatc
     8461 gcaacgattg ccaatagcca aatgacttgt ccagatacat catcaaataa cgatttaaga
     8521 tcaaaaagtt tcatttccat tctcctctaa gttcatttta ttcatttaac gtagtaacaa
     8581 aataactatc gccacttgtt tttacttcca ttgaaaagtt tgattggaac tcgtttcctt
     8641 tctcaaaata gaaacgatac ttcccttgca aaaagtagtt tccttcttta ttttttactg
     8701 catgatccag ttgtacattg atcaattcgc cgccttctaa ccctttttcg ttagaaatca
     8761 atgccaattt ttcatcattt ttgacatata actcaaaaaa ccgtttcaca aatgcttcca
     8821 gcttgatttc ttcgctttca gtgaccgctt gcccagaaga ataaaatcga ttttcctgat
     8881 agttcacaga attggtattc ttactctcag aaagattcaa aaattgaacg ccatcaagta
     8941 cttttaaaac attgttttga taactgactg gaaaagaaag ttgactgctt ttccaatgtt
     9001 ctccttctgt aaaagtaacc tcataaaata ggcgatacat tgcttgtgtc aaatcgattt
     9061 cttctagttt aataaagcga acatctttcg ctactcgatt ggtagccgtc gttacaaagc
     9121 ctagatcaga cgcttccaat ccttgtgcta aataatgatt gacggaagcc tcccattcgt
     9181 ttcgtttttc tggcgaataa gtaaataacc tttcaataaa atgttgtccc tcgtacttca
     9241 attgttccgt agaagacaag gtttggttga cttcagttaa caatgtttct ggattgactt
     9301 gttttttcac tgctaaacgc gttaatgtat ccactcgtcc aaagtttagg aggagcacga
     9361 cacccattaa cagaaacaaa caaaaaaaag cgcctgaaaa tacactcatg gcactttttt
     9421 gcgaaaattg gatcattcga tctgacggtt ctgttttctt tttttggaac aaaaaacgtt
     9481 tcttttgttt cttttccttc tgttgtttcg gtggcttagt ttggaacgct tgtggattaa
     9541 attttgcatt actcatgaga aatgccatac cgttctaaca ataatttcgg tattttgtcc
     9601 acaatcacta cttcgtggac aaccacactt gccccctc
//
LOCUS       DAJSAW010000079         9361 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJSAW010000079 DAJSAW010000000
VERSION     DAJSAW010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.079"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(92..760)
                     /locus_tag="ONT64_002452"
     CDS             complement(92..760)
                     /locus_tag="ONT64_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2303843.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            894..1181
                     /locus_tag="ONT64_002453"
     CDS             894..1181
                     /locus_tag="ONT64_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303844.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1597..3135
                     /locus_tag="ONT64_002454"
     CDS             1597..3135
                     /locus_tag="ONT64_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCR2303845.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3132..4097
                     /locus_tag="ONT64_002455"
     CDS             3132..4097
                     /locus_tag="ONT64_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2303846.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4084..5406
                     /locus_tag="ONT64_002456"
     CDS             4084..5406
                     /locus_tag="ONT64_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR2303847.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5469..5573
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5647..6390
                     /locus_tag="ONT64_002457"
     CDS             5647..6390
                     /locus_tag="ONT64_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2303848.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6405..7397
                     /locus_tag="ONT64_002458"
     CDS             6405..7397
                     /locus_tag="ONT64_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2303849.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7409..8146
                     /locus_tag="ONT64_002459"
     CDS             7409..8146
                     /locus_tag="ONT64_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2303850.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8136..8858
                     /locus_tag="ONT64_002460"
     CDS             8136..8858
                     /locus_tag="ONT64_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCR2303851.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8942..>9361)
                     /locus_tag="ONT64_002461"
     CDS             complement(8942..>9361)
                     /locus_tag="ONT64_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HCR2303852.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3068 a         1607 c         1972 g         2714 t
ORIGIN      
        1 tttttttata aagaagattt tactgaataa tatcatttta gtttacataa tgatatttct
       61 gtaaattaaa taaagtagtg aaaaataaga atcagtgctc taactgttca agaggatcaa
      121 gtggttcaaa tgtttctgta gtctgttttg cttccaattg gggcaaaaca gatccaattt
      181 ccagatactt cactttattc aatgtataag atagaataga acagctaagt aatgtataca
      241 gaaaatgtgt gagatccaaa taaagcaagg agacaactaa aataccaaaa gtaatcaaga
      301 aaagactttt gccaatctta atatgaaaat atttttcaat ggcttgataa atgatatctg
      361 ttccgcctgt gcttccgccg aatcgaaaga cgagaccgca accaatgcca gaaagaatac
      421 ctgtcatcaa actattgatc agtagactat ctacagcaaa atgtatatga aggctctccc
      481 ataaatgtaa aaagaaaact agcgcaaatg ttccgacgat cgtccggaga aaagcgtgtt
      541 ttccaaaaag aaacaatgag ataagcaata aaggtaaatt aaaaagcagt gaagtgacag
      601 aaggagcgaa tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc ccaccatctg
      661 ccagcttaga tggaatattg attcctgtca cagctaatcc gtagattcct gcgcctaaaa
      721 tgattccgca caattctgta aataaattta attttttcat ttctagcctc ttttcttttg
      781 aaaattttca gcaacttctt tattatactg acttatactc ttacttcaag aggaaaatga
      841 aaaaaaatta tttttggctg tttcttttta tttatcaaat aaggagtgga aaaatgcgag
      901 cagcagactt attgacaatt cttacaaaat cacccaaaaa aacagagtat caaggagttt
      961 ttttggctca agaacaaaaa ctggtgcctg tgacaatggt ccagataaat aaagcaaatc
     1021 aatttatctt tttatttgaa cacggcaaag caccacttgc tatgaaagac atctttcttt
     1081 ttttgatgaa gaaccgtgag aaagctgttt attatcagtc aaaagaagaa ctcattcctc
     1141 tttatggagt gaaagagata gaaaataaag tggtgatata agtaaaaata agaaaacaag
     1201 acgtttatga tgtcaaaaca accatttttt acatttttgg caaaaaagtg tcatgataca
     1261 gtataatatt cgttgaacag gccaataaag gtgttctccc ccacgagatt tctactttat
     1321 tggctgtttc tttttattaa aataaaagtt gtgtgtgtaa aaaagatata gctgattcca
     1381 agtgacaatt tcgtatagat ttttatggta ttcgtagtga aaaaacgaga accaaaaagt
     1441 tgatccgact ttgttgcttg gattcttttt ttgtcagcat atttagaggt atttttttct
     1501 tttttttgtt cgtgctgtaa ggttaaggca agacaagtta gttgggaaaa aggaaagaaa
     1561 tgtttatact taaagaataa cagaaaggaa ggtcgcatga ataattttat acaaacatta
     1621 agtgaaagaa aaggcgagtt gctggaagct gcatttcaac atctgtccat ttctctcatt
     1681 tctttgctga ttgcagcact gattgctatt cctttagcga tttgggcagc caatcataaa
     1741 aaaatggcag aagtcttatt gcagatcacg ggtgtgctcc aaacgattcc ttcacttgcc
     1801 ttattaggtt tacttatccc atttgtaggg atcggaactg ttccagcatt gattgcactt
     1861 gttatttacg ctttgcttcc gattttccaa aacacgtata ttggtctagc tgaaattgac
     1921 ccgtctattg aagaagcagc ggtagcattt ggtatgtcca gaatgagacg cttgctgaaa
     1981 gtagagcttc ccatcgcatt acctgtcatt atctcaggta tccgaactgc cttagtttta
     2041 atcatcggta cggctacttt ggcagcattg atcggagcag gtggattagg gacatttatt
     2101 ttgttaggta ttgaccgaaa tacacctgtt ttgacattga ttggtgcgat cagttctgcc
     2161 ttgcttgcaa ttatattcag cggactgatt cgctggttgc agcataagaa acctcgatat
     2221 tccatcatta ctttagcagc agtggtgtgt ttatttggcg gcgtttcttt atatcagtct
     2281 gccttgttta aaaatgaaac catcacgatt gctggaaaat taggagcgga acctgaaatc
     2341 ctgatccaaa tgtataaaga attgattgaa gacaacacaa aaacagaagt tgaattaaaa
     2401 ccgaattttg gcaagacgag ttttttgttc aatgcactgg atcaaggtca aatcgatatc
     2461 tatcctgagt tcacaggtac tgtcctagaa agcttggtga aggtacctga agcaatgaaa
     2521 agtgctgatt tgacaaaaga aaaaacatat gacgaagcag aagaattatt gaataagcaa
     2581 ttcgatatga cattattaaa accaatggct taccaaaata cgtatgcctt ggcagtcaag
     2641 agatcttttg cgaacgaaca tcaacttaaa aagatttccg atttggcagc tatctctgat
     2701 cagatcaaag cgggattcac actggaattt attgatcgtt cagatggata taaaggaatc
     2761 caagaagcat atggcttgaa tttttcaagt gtacaaagca tggagccaag tttgcgttac
     2821 caagcaatca ataatggaga tgtcaatatt gtggatgcct attccacaga tagccagctc
     2881 aaggagtacg atctagtgac tctagaagat gatcgagggt tattcccagc ttatcaaggc
     2941 gcaccgctga tgaaaaaaga ttttgctaaa gaacatcctg aaatcgttaa agcactgaat
     3001 aaattaagcg gaaaaataac ggaggatcaa atgattgata tgaattacca agtcaatgtc
     3061 gaaaagaaaa agccgacaga agtagcacga atcttcctga aaaatgaagg attagtaaag
     3121 gaggaagatc agtgactaaa ttaatcgagt tcaaacatgt gcaaaaaaag tatgatggaa
     3181 agtatgtcat cgatgaccta aatctggcaa tcaaaaaagg agaaatcttt gttttggttg
     3241 gtccttccgg tagtggaaaa acaactactt tgaaaatgat caatggatta tctaaaccat
     3301 cagcaggaga catctacttc aaaggaaaat cgttaaatga atataatctg cagaaaatgc
     3361 ggtggaatat gggatatgtg ctgcagcaaa tcgctttgtt cccgaccatg acagtcaagc
     3421 aaaatattga agtcatccct gaaatgttag gttgggaaaa gcagaaaaga gcagaccgtg
     3481 tagatgaact tcttcaaaaa gttggcttat ctcctgatat ttatcgagat cgtatgccta
     3541 gagagttgtc tggaggagaa cagcaacgga tcgggatcat ccgagctatt gctgcaagcc
     3601 cagatgtgat tttgatggac gagccattta gcgcattaga tccgatttca agaaactcat
     3661 tacaagaatt ggttttgtcc ttacacgaag aattgggcac gaccatcgtt ttcgttacgc
     3721 acaatatgga agaagcaatc aagctaggtg atcgtattgc atttatgaag gatggagaaa
     3781 ttatccaatg tgacacacca gagcagttat tgatgaatcc taaaaacgac tacgttcggc
     3841 atttctttga tgaaccgaaa cagacgaaag agtggcgagt agaagattta gtcgtcaatg
     3901 gatatttctt aaatgaaata ccagaaaatg ctcgtcaaca agtttgcttt gatacgccta
     3961 tgaaagaagt atattcgctt ctttcggtgt atccaagcat tgtgatagta gaaaagcaaa
     4021 gaagtattgg gtcgctgaca agtaaagaaa tctttgcttt tttaagtcga gaggaggaag
     4081 gaaatgaaaa catatgatgc aatcatcatc ggttcaggtg tgagcggctt aagtgctgct
     4141 tatggattga aagaagcagg taaaacagta ctagtggtcg aagaagatct atggggcggg
     4201 acttgtccta accgtggatg tgatcctaaa aaagtcttgt taagtgcagt agaggcacgt
     4261 aatcgggtaa aacaattgag tggaaaaggt tttaacgaga tcccaactgc caactgggaa
     4321 gaacttcaaa aattcaaacg aacatttacg gatccagtcc cagaaagccg aaaaaaacaa
     4381 ttagcagaag ctgaaatcga tcatctttca ggaaccgctc gttttttgga tgattcatct
     4441 attgaagtca acgaagaagt cttccatgcg gattacttag ttttggcaac cgggcaaaga
     4501 cctacgatct taccagtgga gggaaaggaa tacttgaaaa ctagtgcaga ctttttatca
     4561 ttgcctgtac tgcctaaaga aatcattttt atcggtggtg gttatattgc ttttgagcta
     4621 gctacgattg ccaatgctgc agggagcaaa gtaaccattg tccatcataa tcaacggcca
     4681 ttaaaagaat ttgaggcgtc tttggtagag gaagccgtcc atcaaatgga agcatctggc
     4741 attcagtttg cttttggtgt tgaaacccaa aagatcattt cagaaggtac tcgctatcgt
     4801 ttagtcggaa aagagacgga attagtagca gatatgattt tttgtgcgac tgggagacag
     4861 ccgaatacag aatctttagc acttgagcaa gcgaatatcg tctttgataa acacggaatt
     4921 gcagtcaatg attatctgca aacgagcaat ccaaaaattt ttgcgtgtgg agatattgtt
     4981 tcaaggaaaa ccccaaaatt gacaccagtc gccacttttg aaggaaatta tgtagctaag
     5041 cggataacgg atgcaacaag cgaacctatc aaatatccga ttatacctac gatcgtctac
     5101 gccagtccta aacttgctga agtaggagtt acaaaaagtc acgcctcttc gtcagatcaa
     5161 gtggtagaga tggatcttac cagttggttc acctatcatc gtgtcaatga accagttgct
     5221 aaggctgaat tgacctttga ccagcaaaat tatttgattg gtgctgcagt gattagcgag
     5281 caagcagacg aattgatcga tgatttgaca ctcgtcatca atcaaaaatt gactaaaaaa
     5341 gagttggata gctatatcat ggggtatccg acattggcaa gtgatctctc ctatttattg
     5401 aaataatcag catgataact aaagcgcttg ttgctttttc cttcttgtgc tacactttaa
     5461 aaaatgaata cctgtttctg gggatgtcgt agacgacatg agagtaagtt gtgaacttag
     5521 acccattgaa cctgtcagtt aatactggtg aagggagaaa catagcagac gcttgaagcg
     5581 ggttactatg tcgtgcccat ttcaagcgtt ttttgttatt catattagaa gaaaggaggt
     5641 aacaaaatga agaaaaatct ttatccagca gtcagtatgg cattcatttt aattatttgg
     5701 cagattgttg tcatagtttg gaaaatacct gcctttattt tacccggacc tgctgctgta
     5761 ttttctgcat tagcggcaga tgcacaagta ttactttccc attctgtcac gacattaaat
     5821 gaagcagtgc ttggtttggt aatagctagc gttctttctt ttattactgc cttggcaatg
     5881 gattattgga agtttttaga aatgtctttt tatccgttac tagtgatttc acaaatgttg
     5941 ccaatcatgg tgttagggcc attacttacc ctatggtttg gtttcggtat gcttccaaaa
     6001 gtgatccttg ttgtcttgat aagctatttt ccgatcgtag tttctttttc ggatacattg
     6061 aggaaagtat caaaagagca aatcatcttt ttgaaaacga tgggggcaga tacagggaag
     6121 atttatcgaa tatataaaat accagcgggt ttatccggct tcttttccgg tttaaaagtg
     6181 gctgctactt attgtgtcag cggagcagtc gttggagaat ggctaagcgc tcaagcaggc
     6241 ttagggtatt acatgatacg cgtaaaaaac agctaccaga tcgataaagt ttttgctgcg
     6301 atcatttgtg tcattctatt gagtttattg ctgaatggcg cttgttcatt attgaaaaaa
     6361 gggtattatc gtatgcttta tattttttaa gagaagggga tcaaatgaaa aaattatggc
     6421 ttttattacc gctactgttg cttttatccg cttgtggcac tgcaaaagaa acaagtagca
     6481 aacaagaaat aaaggatttg aaaaaagtga cacttgtttt ggattatgta ccgaatacta
     6541 atcatactgg catctaccta gcaaaagaaa aaggctatta taaagaggct ggtttaaatg
     6601 tccaaatcat cgaaccagga gataattcga caagtattgg tctagtaggt gcggacaaag
     6661 cacaatttgg tgtaagttat caagaagatg tcacttatgc tcacgcagat ggacagaata
     6721 taccagtcaa agcaattgct acagtgatca aacataatac ttctggattc gccaccctat
     6781 cagacagtaa catccattca ccaaaagatt ttgaaggaaa aacatatgca ggttggcaaa
     6841 gtccaagtga agaagctgtc ttaaaagctg tgatggaaaa agacggcggg gacttttcaa
     6901 aactgacaat ggtaggaagc aatggagaag ggccagaaag cttaggaaaa tcctcagata
     6961 tccaatggta ctttgaaggt tgggatatga tcaaagcaaa agaagcgggt attgaactaa
     7021 actatatccc attgaaagaa ttagatgaac ggttagatta ctatactccg gtaattatta
     7081 cgaatgatca actgatcaaa agtgatccag aattggtaca gtcttttatg gatgccacga
     7141 aaaaaggcta tcaggaagct attaaggatc caaatgacag tgctaaactg ttgcaaaaat
     7201 atgcaaaaga aaatgatcgg acgtttttag aagaatcaca agcattcttg tcaaaaaatt
     7261 atacggatga tcctgagaat tggggattga tggaagaaaa agtttggcgc aattatacag
     7321 cttttatgca ggaaaacgga ttgatcaagc aagatgtacc ttcgcaagaa ctattcacta
     7381 atcagtttat aaaataggag tgatagatat gtcaatccaa atcagccact tgaatgtagt
     7441 cattgaaaac ctgaaaatat tagaagacat ccatttcact atccctaaag atacctttac
     7501 atcgattgtc gcgccaagcg gagccggtaa gtcaacattg ctgaaaactt taacaggagt
     7561 ccagcctatg acttctggaa cgatcaaagt agacgatcaa aaagtaactg gtctccatac
     7621 agcatttagt tatatgccgc aagaagatat gttacttcct tggctgagcg tctatcaaaa
     7681 cgttacgtta tatcaaaaaa tcaatcatct aacaatcaat gaagaacaag taagggatta
     7741 tctggatatc tttgggttaa acggctatga acattttttg ccggaacaat tatctggcgg
     7801 gatgaagcag cgaacggcct tgttacgaac aatcatgaat ccttcttctt acttgcttct
     7861 tgatgaacca tttggagcgt tagatgctat gaccagaggg cagatgcaag actggatgtt
     7921 gaagctgcct aaaaaagcaa agcggactac tttacttgtg acgcatgata tcgaagaagc
     7981 aatttattta tcagacagga ttcttgtgct ttccgcccgt ccggcacatg tgattgctga
     8041 gattcaagtg cctgaaaaac gacggtcacg tgaatggcta ctgcagcaat cagagttgaa
     8101 gcaaacgatt tatcagttat tggcaggtga attagatgtt aagtgatgct cattgtcatt
     8161 tagatgggag tcagagttta gcactccttc agcaggaaca cacgattttg acgatcatca
     8221 attgtgattc gccagaagaa tggaaggaaa acagacagct tgcagcaagt aagactcaag
     8281 ccttaagtta cggtatccat ccttggaaag cagatagcta tacgtttgaa caagttgagc
     8341 cttttttgaa aaaagcaagg atcatcggtg aaatagggtt agataatatt tggacgaatg
     8401 taccgatgac tacacaaaaa aaagtatttg agcgacagct tgcttttgca gccataaatg
     8461 agaaacctgt tgtactgcac acaaaaggat gcgaaaaaga gatcttaaat tatatccaag
     8521 cttaccctaa tcactatttg atccattggt attcttcttt ggactatcaa caagactata
     8581 ttgatttagg ctgttatttc agcattggtc ttgatttgaa aaagaatcca gcagtctggc
     8641 aactagctaa agcagtccca atcgaccact tacttattga aacggatgga ctacatgcag
     8701 cagagtgggc tttagggggg attttttcag aaaaagatta tgtctcactg cttgaagaac
     8761 agttaaagac aatcgctgag atcaaagagc tatcagttga agaagttaaa aaaaatactt
     8821 atcaaaactt ggaacgtttc attagattag gtaaataaaa aagagatgtg acacaactag
     8881 tgtcgcacct ctttttggct ttctttgtat aaaagtaaac tgtttgatta ataaatgaat
     8941 cttagtacca accgtttgct tcccagaatg ctttcgccgc ttcccaagaa ccataacgtg
     9001 aagtaacata ttgttctgct actctttctt ggtttgcagc agagtagtca ccatttaagt
     9061 aagaagaatc taattggtaa cgtccgatgt aacgtccgtt cgttgctgtg taagaaccgc
     9121 ttgattcttt ttgtgcgatc cattcttttg cagagcttgt gcttgctgga gtagcagcag
     9181 tttctgttac agcaggagca ggttgtgttt cagcaacagg cgctgcttgt ttttccgttt
     9241 gaaccgtttc ttgtacaaca ggttgttcag cttggactgg tgcttcttga acggctgctt
     9301 gaggttcagc agctgcttgt gcagagcctt ctgttgggat tgttaattgt tgtcctgagt
     9361 a
//
LOCUS       DAJSAW010000080         9281 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJSAW010000080 DAJSAW010000000
VERSION     DAJSAW010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.081"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..435
                     /locus_tag="ONT64_002462"
     CDS             <1..435
                     /locus_tag="ONT64_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HCR2303853.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ONT64_002463"
     tRNA            complement(759..842)
                     /locus_tag="ONT64_002463"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ONT64_002464"
     CDS             1370..1870
                     /locus_tag="ONT64_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2303854.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ONT64_002465"
     CDS             complement(2060..2404)
                     /locus_tag="ONT64_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCR2303855.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="ONT64_002466"
     CDS             complement(2558..3478)
                     /locus_tag="ONT64_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2303856.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="ONT64_002467"
     CDS             3665..4156
                     /locus_tag="ONT64_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HCR2303857.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="ONT64_002468"
     CDS             complement(4180..6555)
                     /locus_tag="ONT64_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR2303858.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="ONT64_002469"
     CDS             6881..7507
                     /locus_tag="ONT64_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR2303859.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="ONT64_002470"
     CDS             7530..8234
                     /locus_tag="ONT64_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR2303860.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="ONT64_002471"
     CDS             8426..9259
                     /locus_tag="ONT64_002471"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR2303861.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2828 a         1791 c         1651 g         3011 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct t
//
LOCUS       DAJSAW010000081         9135 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJSAW010000081 DAJSAW010000000
VERSION     DAJSAW010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.082"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..83
                     /locus_tag="ONT64_002472"
     CDS             <1..83
                     /locus_tag="ONT64_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR2303862.1"
                     /translation="SVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            74..859
                     /locus_tag="ONT64_002473"
     CDS             74..859
                     /locus_tag="ONT64_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HCR2303863.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            828..1544
                     /locus_tag="ONT64_002474"
     CDS             828..1544
                     /locus_tag="ONT64_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR2303864.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(1646..2680)
                     /locus_tag="ONT64_002475"
     CDS             complement(1646..2680)
                     /locus_tag="ONT64_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HCR2303865.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(2684..3742)
                     /locus_tag="ONT64_002476"
     CDS             complement(2684..3742)
                     /locus_tag="ONT64_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCR2303866.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(3962..5110)
                     /gene="nagA"
                     /locus_tag="ONT64_002477"
     CDS             complement(3962..5110)
                     /gene="nagA"
                     /locus_tag="ONT64_002477"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR2303867.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(5186..6022)
                     /locus_tag="ONT64_002478"
     CDS             complement(5186..6022)
                     /locus_tag="ONT64_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2303868.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(6206..7423)
                     /locus_tag="ONT64_002479"
     CDS             complement(6206..7423)
                     /locus_tag="ONT64_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR2303869.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(7572..7751)
                     /locus_tag="ONT64_002480"
     CDS             complement(7572..7751)
                     /locus_tag="ONT64_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303870.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            7806..7889
                     /locus_tag="ONT64_002481"
     tRNA            7806..7889
                     /locus_tag="ONT64_002481"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7840..7842,aa:Leu,seq:gag)
     gene            complement(8129..9127)
                     /locus_tag="ONT64_002482"
     CDS             complement(8129..9127)
                     /locus_tag="ONT64_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303871.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         2907 a         1841 c         1519 g         2868 t
ORIGIN      
        1 aatcagtgat aatctatatg acgttttgtt taaagagacg attgagcatc aaacgaccgt
       61 tcggactcaa tccatgtatt taaatgggat agaaactatt tctagcgtta cacctctttt
      121 tagcaatgac cgttttttag gagcagtcgc aactttacga aatcgatcag aagtaatgaa
      181 attaatggag gagcttgggg ggacgaaaca atatataaac tccatgagag ctcaaaccca
      241 tgaattcatg aataaacttc atgtcatttc tggtttgatt gatttgaaga agtatgaaga
      301 agtcacccat tttatccagc agctaaatca aaattataaa aaagagattg gccaagtctc
      361 aagtctgatc aaagtacctg caattgccgg ttttttatta ggcaaaataa acgaagccaa
      421 agaacaatct gttgaaatgc tgttagaaag cgattctttt atccctaaaa tggagatgga
      481 tgactccatc cattcacttt tgcaagtgtt agggaactta cttgataatg caaaagaagc
      541 cgtgctacaa aaacatgaaa gaaacggaca ggtccggatt catttaaact atgacacaga
      601 agctgaagta ttcatcgtga ccgtctgcga tggcggtgtc gggatatcag aagaaatctc
      661 agaaaaaata tttacgctag gtttttcgac gaagggaatc aatcgtgggt ttggattgaa
      721 tctcatccat tcaatcgtca aacgccacgg tggattttta gaattttcca aacaaacaaa
      781 tggcggtaca atcgtctatc tagaattacc aaaaaaggaa gatcaaaatg atgacaaatg
      841 tcctgataat cgaagatgat ccaatggttg cagaattgaa tcgtactttt gtggaaagca
      901 tccctaatat gaacgtaatc gaaagtttca gaaatgtaga agaaggaaaa aaatatttca
      961 gacaaaaaaa cgcagatctg ctgctgcttg atgtatacat gcctggcgaa agtggtatcc
     1021 attttttatc ttggcttaga cagcaaggaa atgatatccc agtgattttg atcactgctg
     1081 cagatgatat cgaaaccata aagaatgtga tccgctacgg tgccttagat tatttgatca
     1141 aaccgttcac tttgcaacgt tttcagctgg cgatcgaaaa gtatcttgct ttctctcaga
     1201 caatccaaac gactgaaaaa gccgatcaga aattgatcga tcaagtattc aaccacacca
     1261 ctcccattca cgaaatacca tcattcatcg atttgcctaa aggtctgtca aaactgactt
     1321 taaaaaaaat aagtgcattt attctaaaag aagatactat tttttcaaca aaagaactgg
     1381 ctcaaaaaag ctcactgtca cgtatatccg tcaagaaata tttgaatttt ttagtagaac
     1441 aagatatatt gaacgaaaga atcaaatatt tggaaatcgg ccagccgcta acctgttatg
     1501 aattgacaaa gaatgggcgg aaaaagctca cagattactg ttgagtcatc gaattatccc
     1561 atacaaaata gtccaagtat acagaagtaa caactgttta cttggactat ttttaaacat
     1621 tttattgacg aatatccatg ttttcttatc ttaattgacg ggctttagga agagatacct
     1681 catttttcgt ttttccagtt tctattattt cacgaaagtt ctcgaaactt gtcgaaatca
     1741 tatttgataa tgcctcatct gtatttgaac ctacatgcgg ggttacaaga actctagggt
     1801 acatttctac taattgctgg atagcagggt ctggaatcgt ctcccatggc ttgaaggatc
     1861 ggaaaaaaac ctctgtttca tttgcaaaaa catcggttgc aaaaccagct aaatgattgc
     1921 ttttcaaagc tcttaaaata gcttcattat cttgaagctc tccacgggct gtattaatca
     1981 agatgctacc ttttttcatt tgagctaaaa atccttcatt gatcatttga tcgttttcac
     2041 ctgggatatg aggtacatga atactgacaa tatcgctttt ggctaataac tcttcaagcg
     2101 agtggaacgt caatactttt ttagcagctt ctgtctgatg aatatcgtag ccgatcactt
     2161 tggctcctag tcccttaaac aaaacagctt ccgtcaaccc tatcctgcct gttcctatga
     2221 tccctactgt gcaatttctg atttcacgac taaacatttg ctcatccact cgaaaatctt
     2281 tgaaagatgt tcggatcgtc gtatacgcag tatttctcaa aagcatcatg gcaaaagtca
     2341 aagataactc tgctatcgca tttggcgaat acgatggtac acgggctact gtcataccaa
     2401 aatcagctgc cgcttgtaga tcgatatgat tgactcccac agtccgtgta aaaacataac
     2461 gaatcccata ttcctgcatt tttgcaagat tggtctcatc tgctatacaa tttcccctca
     2521 acaatacagc atcatggtcc tttgctgaca cgatattgtc atgtgtcaac agttcttcat
     2581 aaagactgag atcataatgg taaatattca atttttcaaa aaaatctatc tcgttcggac
     2641 gaacaccgta acatgcgatt ctcatacttt cttcctccat ttcttatgaa aataaaccta
     2701 tgccatgaat catttgtaat acaacgatcc atacaggtgt cagtaccaca gcaaaaatag
     2761 ttgacagtaa ggatgcattg gaggctaaaa gtgcctccct atcaaaactg atagcatagg
     2821 ctgctgccac tgttgcagct ggtgtagcca tcatgataac gattgttgcc agtgcttcaa
     2881 aattgactgg tagaatattc gttgccgtca aaactgctag aatcacgata ttcaatgcag
     2941 gtacaaatac cactttgact agactgtaat accatgaagt tttttcagat gcagcttttt
     3001 tgaagcttac ttcacctaat gttgcgccga ttgctagcca cgccaatggt gaacttaaac
     3061 cagctaaata atccatcggc tgaaatagcc acggtgcagt ctggtcaatt cgcaaaaacg
     3121 ctacttggct tatctctcct tctgcacctg taactgacac ttgtggcaga tacccttgga
     3181 acagccaaat gaaaaggccg gcaaaagtag cgataatgat tggattgacg aacatcgctt
     3241 tgatattttt agcttccatc ttcaagccac tcatttttat gtatccataa gaatacaaaa
     3301 agattcggta gccaatattg aaaattgagg aatacatcac gccgacagga ccataaatcg
     3361 cactgacgat aggcgttccg aaaaaggtcg tagaaccaaa gatcgtcagg acacgcaacg
     3421 ttgtttgctt atccccttta tagcttaaga aaaaaggttt tgaaactaga ataagaataa
     3481 tataaataac taatccccaa atcaagacat tgattccttg catcagtaat tctgtattga
     3541 tatcttgcat gaaagctcga aatgccagtg ctggcaaagc cactgttaag acgactttcg
     3601 atagaatttt tccaacagat tcagaaaaaa tccccttttt tctacaaaca aaccctaata
     3661 atataataaa aactgttgaa gtgattgcac tgacaatcga catatctgtc aaagtcgatc
     3721 gaaaaatgtc aaataagttc atctttctcc tcctaattat gttcatttat tcacaataaa
     3781 aaaagctttt attttgctaa tacgtattaa catttttaat tttatcttta cattgttact
     3841 tattgaacaa taggaaatta aagaaacttt tgaaacttat gtaaagtgat ttttactaga
     3901 acacaaaaaa accttaaaac caaacttaag aagtttagtt ttaaggttaa cttaaattat
     3961 attatgcgtg gaaacgttcc acgccatcta gataagttgc ttccaatgtc atatcagggt
     4021 tcaatacgat aaagtctgca tcgcgtcctt gagcgatttt tccacatgta tcatcgattt
     4081 tacagctgat tgctggaatc agactagcca tcatgactgc ttgttctggt gttgctaatc
     4141 cccagtctac aacattttta attgcttctt tcaatttcaa tatacttcca gctaagttgc
     4201 cagattctag acgtgctgtt ccttctttta ctactactgg aaattcgcct aaattgtaat
     4261 ttccatcagg catcccaccg gccatcatac agtctgtaat taatgcgaca tgatcatgtc
     4321 ctgctttttc cattaggatt cccgcagcat ttggatgtac atgatggcca tcacaaatca
     4381 attcagaaaa gacatgttct aaagataata acgcacccac catacctggt tcgcgatgat
     4441 tcaatccacg cataccattg aaagcatgga caaatacact tgcgcctgct tcaacagctt
     4501 tttgcgcttc ttctagtgta gcattactgt gtcctagaga aacaactact ccttcttcag
     4561 tcacttgttt gacaaactct tctacacctt gacgttccgg tgctaaagca atttttttga
     4621 ttaaaccacc tgaagcttct tgccattcat ggaaagtatc caaatcagga tccccgaaat
     4681 aacttgggtt ttgtgcccct ttgtgttctt ctgtgaagaa aggaccttca aaatagattc
     4741 ctttgatttt agctcctggt gcttcctgat acattttgcc gattgtctca gctacatctt
     4801 ttaaacgttc tttgcttgac gtcaatgttg ttggtaaaaa tgaagtcact ccacaagata
     4861 acagaccttc agacatcact ttgatgcctt ctgcatcatt atccattaca tcatgattca
     4921 tatagccgtg aatatgtgta tctaccaaac caggagcaat ccatttgccg ctttggtcaa
     4981 tgatgttgac ttctccctca ggtagttctt tggtgtattc accaaaaagt ccatcggtaa
     5041 tttcaagata gcctgcatgt ttgacacctg acgctaaaaa gaatttgtca gcatagataa
     5101 aagttctcat tctattctct ccctttgttt ttctacctct aaagtactct tatttagaga
     5161 agaagtcaag aatggtctac accatttaca aattttttga aatttgtttc acagcttgtg
     5221 ctaagccttc atagaatgct gctctttctg gttcttcttt acgcttgtat attaagcttt
     5281 taagcacaaa taaatcattg aggtaatagt agctattctt ctcttgagcc caatagattc
     5341 cttgattcac ctgttctaac gcttcgtcga tttcattttg atggaataag aaatttccat
     5401 aattataaaa aatttgcgaa aattcacttt ttgatcgttc atcagctgat tgatgcagca
     5461 gttggatgct tcgactcaaa tagtagcgtg cttcagccgt ttttcctgcc acaccatata
     5521 tttttccgat acaactaatc agttgtattt ctgtgtcaga aacatggatt ctttcttttt
     5581 ggctagagta gcttaatgct tctttcaaat aataaaggga ctgagagata ttttgacgca
     5641 acgtgaattc gcaaacaccg agataataat aatagcgttg aaagtcagtt gcttgataca
     5701 gattttcaga aatatcagaa gattttagta cttgttctaa cttccgataa tcacgttttt
     5761 ggaaacaact atccaacagt tcaaataatt tatcgtttgt ccgagtagtt tttcgagaga
     5821 gattcatcac atgatcaatc gttaccccta gccgttgaca aatctgcatc atcaccaata
     5881 cattcggcag ttcctcatta ttttcgatcc gacttaatac actttgtgaa caaatatcat
     5941 gcgaaagcat tttttgcgtc attttttgtt cttttcgaat ctctttaatg atcgttccaa
     6001 aggactcgat taacgtttcc ataagtatcc tccccaaaat atgcgtattc gcattctatt
     6061 cgtattttac tttaggaagt cgcaaacttc aagaaaagac ttgcaactaa ataatatact
     6121 ttagttattt aagaaaactc cactagaaag agagcctcag tgtgtttgtc tgtggccctc
     6181 ctactgaaaa atttggtttt taaaatcatt tttggcgata ggttgctagg aattctctca
     6241 ttttcttagc tgtttgtttc aaatcatcca ttttaggaag ataaacgatt cgaaaatgat
     6301 ctggctggtt ccaattaaat ccaccaccat gtacaagtag aatatgatgt tcatgtaaaa
     6361 agtccaaaac gaatttttca tcatccaaaa tattgaatcg ctttgtatca atttttggga
     6421 atatataaaa agcagctttt ggttttacgg ctgaaagacc aggaatatca ttgattgcat
     6481 tatagatata ttctctttgt tcatagatcc ttccgcctgg caggagcagc tcatcgacac
     6541 tttgataacc gcctagcgcg gtttggataa tttgctgaga caatacattg gaacacaaac
     6601 gcatggagga caacatattc agcccttcta tatagccttt gacgtgtgac ttatctccac
     6661 ttaggaccat ccagcctact cgaaatcctg ctacacgatg agatttggat agaccgttca
     6721 gcgtaacaac gaacaaatca ggcgctaatg tagcaatcgg aatatgttct agaccatcca
     6781 tcactaagcg atcatagatt tcgtcggaat aaataatcag gttattttgt cgagctactt
     6841 caacgatttc ttctaataat tcttttggat aaagtgcccc agtaggatta ttaggattaa
     6901 tgataacaat agcttttgtc cgactcgtta tttttgattt gatatcatca atatcaggat
     6961 accattctgc ctcttcatcg caaatataat gaacaggtgt gcctccagct aatgcaatag
     7021 aagccgtcca caacggatag tccggcattg gaaccagtac ttcatctcca ttgtccagca
     7081 acccctgcat acacatcgtg atcaattcgc tcactccgtt gccggtgtag atatcattga
     7141 tcgtcacatt cggaaaatgt ttcagttggc aatattgctc aattgctttt ctagcagaaa
     7201 agatcccctt ggaatctgag tagccttctg agtcacgggc attcatgatc aaatcacgaa
     7261 tgacttcgtt tggtgcttca aatccaaaag gtgcgggatt tcccgtgttc aattttaaaa
     7321 tactaatgcc ctcttcttgc atccgctccg cttcttctag aacgggaccg cgaacatcgt
     7381 aactgacacc ttctaatttg ttggattttt caaattctct catcacttag ccaatccctc
     7441 atttcaaaaa gttagaactt tccaaatttt ttgttattta ctatttcaat atcatactcc
     7501 atcctagaaa attttgccac ttatttagga aaaacgtaag tattaaaaaa acacaaacaa
     7561 gcgttgagaa cttaattgtc atgatagtca ttccatagat tcgctgccca atccattgca
     7621 attccacaaa tgattcctag cataaaacca agcaaaaaac gtccgaatgc aagtccaatc
     7681 aatacaccta agatcattcc cagtcctgaa aattctccgt catatttctc catcttaata
     7741 agtcctccca tttttgagct tgacaactat gtaaaacctc gctatcatta gtgtgttatt
     7801 attacgcaga tgtgttggaa ttggcagaca agcatgattg agggtcatgt gagcgttagc
     7861 tcgtgtgggt tcgagtccca tcatctgcac tacaactcgt gaagttaatt ttgcttcacg
     7921 agtttttttg tttgaacgtt tctttatgcc attatataca tatcactgta tcctttacaa
     7981 aaagaacgta caatcatgta aacagcctga ggtgagtata tgaaggtcgg aaacaagcta
     8041 ctcgtagaaa cttgcaagtg ttaaaacaat taatcgaaaa acgagtaaaa tccctcacct
     8101 aatactaaag cgagggattt tttgtatatt atctgtttat caccgttatt ataaaggtac
     8161 aaataccaag cacggttaat ccaaattgca taaattcatt tttttgtaca ctcatgcttt
     8221 tcattagctc tttaactgcc attagtacta agatggcgat aacaacataa agtatatagt
     8281 acgtccgcat tccggaagcc catactgtgg gagaaaatcc cataattaac cttgaacaaa
     8341 atcccattat catcaaaata actaaaaaaa tagaagtttt cttattgtca aaacttaaat
     8401 aaattccaac aattaaacat agtaataatg ccaagactac taaagtagct atccaagtac
     8461 ctggataaaa aagtgacagt ttcgttccta actctgtaaa gagattattc aggttactac
     8521 tactccaaat cattgccctt ttatttccat tgacgtaagt aaagctttga cccattgtat
     8581 taccaaaata aataatcagg tttaagaaaa acggtaatgc agtcaatata cgcacataat
     8641 agttttgaca tttcctatat gttagaagga aaattaagaa aaatagcaat aaaaataaga
     8701 tattcatgtc taaaaaaaac ggctttccaa atgatgaaat acctaaatct aacttattaa
     8761 gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca gcacgtaatg
     8821 cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt gaaaatactg
     8881 ataacttata attatatcgt ccattgacaa tcaaatataa actaactata cttgttagaa
     8941 caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa ggaatactta
     9001 cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac ggataaaaag
     9061 ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc caaccggttt
     9121 cccccattac tgtca
//
LOCUS       DAJSAW010000082         9106 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJSAW010000082 DAJSAW010000000
VERSION     DAJSAW010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.083"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            105..1406
                     /locus_tag="ONT64_002483"
     CDS             105..1406
                     /locus_tag="ONT64_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR2303872.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(1536..2264)
                     /locus_tag="ONT64_002484"
     CDS             complement(1536..2264)
                     /locus_tag="ONT64_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2303873.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(2277..3557)
                     /locus_tag="ONT64_002485"
     CDS             complement(2277..3557)
                     /locus_tag="ONT64_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2303874.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(3698..4300)
                     /locus_tag="ONT64_002486"
     CDS             complement(3698..4300)
                     /locus_tag="ONT64_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCR2303875.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            4545..4751
                     /locus_tag="ONT64_002487"
     CDS             4545..4751
                     /locus_tag="ONT64_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303876.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            5063..5782
                     /locus_tag="ONT64_002488"
     CDS             5063..5782
                     /locus_tag="ONT64_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR2303877.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(5863..6546)
                     /locus_tag="ONT64_002489"
     CDS             complement(5863..6546)
                     /locus_tag="ONT64_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HCR2303878.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            6764..8950
                     /locus_tag="ONT64_002490"
     CDS             6764..8950
                     /locus_tag="ONT64_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR2303879.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         3220 a         1582 c         1706 g         2598 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
     1441 ctcactctat cagtcctatt tgacaaagag ccaaaaaaga accgaagggt ttggatcggc
     1501 tggtcattta gtcggtccaa actattcggt tctttttatg aaatcaattt ctttaatcca
     1561 cggatagagg ctgggatggt ctcgccgttt gccaacgtga cttctctggt ttttttatta
     1621 atcgaccgca cattttgtgt attgataaca taggatttat gacaacggat aaaattccga
     1681 tgcattttct cgatttcttt gatcttctca tagaattgta attgggcatg agtcaaatgg
     1741 acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat attggatgtc tttgatcggt
     1801 aaaaatttga tctcatcatt aaactctatt ttgaagatat ctttttggat ttgggacatt
     1861 tgaaaatctt cgaaaatttt gctcaggata tgctcgacat tttcttttaa atcataaaga
     1921 tcttctttga cgatataatc agtcggttcg atgttgcttt ttaagatttt catcgacatt
     1981 tccgtatggg aagtcacata gattattttt cctaaaggat ctaattcacg gatcaattta
     2041 ccgatcgcta caccatccat ttcactgtct tctagttcga tatctaaaaa gtaaatacca
     2101 aaagaagaag ttctgatctt ggctgcattg agaagttctt ccggatcaga ggtagaaata
     2161 tggacataac tgtctaagtt ttcaatcatg attctatttt tgatcaccgt tgataaaact
     2221 tctaattgtt ttggatcgtc ttcacaaata taaatgggaa tcatatcatt actcctttat
     2281 tttatttgta tataaatcgt ttgagaaaaa cgctggtttt caattgatgt ttcaaggaaa
     2341 agataaggat tggtctttgt caattcatcc aagataaaca agccaaggcc ttcgtttttt
     2401 ggatttgttt ttgttgagaa ccctttttgt ttcaactggt ttaacggtaa tgtatttgtt
     2461 gttgtattga tcacacgaat catcacggaa tgattcttct tgagtatggt caattcgatt
     2521 tttttcttct ttgcagcact agctccttca atcgcattgt ccaagataat accaagcata
     2581 cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg gctcaggaaa atgaagctgg
     2641 acgatcaacc ctttttcctg tgccatcatg agttttaatg acagtaaact acggatctca
     2701 ggaactttca tattatccaa catacttaga ttggccggaa aaaggttcat catctggcgt
     2761 gtaggtaata tcgtttgatc ataataagac cgtaatcctt ctaagtcgcc ttctgaaata
     2821 tagctgtgaa gagaaaaaag cagattttgg taatcatgtt tgaattctct caagtcttga
     2881 tagttttttt ctagatcttt gatataatcc tgttgcatcc tgacttgttg ctctaataac
     2941 tgatcagcta atttcttatt agagattttc caataataaa ccaagatgat caggccaaca
     3001 cacagatata gaatgaaaaa actcgtattc aaagctaaga catcaggtgt ttcaccagaa
     3061 aaacgggtat aaaaaataat gatatagtat gtaaataata tcaatgtccc gatcgtaccg
     3121 atcatacggc ggtccacagt gtgaaaccaa gtctctaacg atttttttaa acaaaaagct
     3181 aataaagcag ataaaaaagc ggataagaca atgtgaaaat actgcaatga atctcctggt
     3241 tcaataaccg aagatttcaa aacaaaaaaa tcgtcgatgc ttgttaagtg atctcctaaa
     3301 agcgacgtca ataacccaaa aggaagcgcg taaaccaatt gttgacgcga agtcgaaaag
     3361 aagaaaaaag taagcatcag cattcctata gcaattagag cagtaaaaat ccctaaaatc
     3421 gaatagaccc atccaatcaa aatacaagaa atcagaatga ctcccgcaaa cagatatttg
     3481 tatcggatgt gatggtcaat caataagata caaattgtgt aaatcaaata ttgcaacgct
     3541 atggaccata aaaacataaa gtacagctcc cattgatttt taccaaatta tagcacaatt
     3601 agcgtattat ttttttaatt tttcagggat tttgaattca gaactcatac caaagcagca
     3661 gccagataca aaaaagtcag ataagttagt taattgttta atgattgttt ttttcatgaa
     3721 agattcctcc aaaattcatc aagtagtttg taataggaag caacgtagct atttctaaaa
     3781 caatcccaag tagcaaatag gtagaaaagt aagaagctga gaaaaataaa gtaatgagag
     3841 aaaaaattag aaacatgctg atcgccttct ttaataatgg gggacgtttc tcgggttcaa
     3901 tgggattgat tgctgtacct cttgatccat agcgaagcag taacaaaaag cagctgatta
     3961 acaaaaagac catcattggg aaggagatag tcaattgcag atgtttcatc accaagggaa
     4021 cgccccataa ataaagtagt gtccacagca gacaaacgaa ttcagagtcg gaatgcaatc
     4081 cataagcata tctgcggatg cagatataag aaagaaaggt gattcctgtt tccgcccatg
     4141 agcctgtcac cagagaaaag actagaacga ccgctagttt tccaagatta ttcaataaaa
     4201 cttctaaggc aaattttact tggacataca ccatatcttc ttccgatttg tctccgctga
     4261 ataaggcttc taataatttg ttcacaactt tatctttcat ctcatacgta cctccaccac
     4321 aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc ataaaaagtg gttttgacat
     4381 caaaaccgtc atgatctcct aaaaaaatct tcgttttcca agaaccttac caaaaaaagt
     4441 gacagttttg atacgaaaag tactttttat gacaagtttg tgctttttcg agtgatttat
     4501 gttaaaaaat tactgacctt ataaaaggtg atggagggat tcttatggaa aatgaacaat
     4561 tgtttgaagt cggcgaaaca tatgcattta aatctgattt atttgaatca ccaataaaag
     4621 gaaaaataga aaaaatctat gaaaatagtg tgatgatacg tgtcatgaaa tgcgcagtag
     4681 aagacaatga taccgctcat aatctgcact ggcagttagt tgtacgcaaa tcaagtacaa
     4741 tagaagaata aaaacagata aaatgttgag agcataaaga ggttgtgagt ctcccgttta
     4801 gctctgagtt acaaggaata atgttaaaaa acagcttttc atgttttatg aatattgcct
     4861 tgtgaccgag gtgttggcag gagaatactg tttatttgaa aaaagggctg cgacaagact
     4921 tttgtcacag cctttatttt catgaatagg agttactttt ggctactttt agagaagtag
     4981 cttttaagga gcagataaag tcaaggcttt tatttctata aatgaagtag taaaatgaag
     5041 aacagatttt attacaggaa ggatgccgat aattgtgatc attgttgcag gaacgatcgg
     5101 tgcagggaaa agcacactaa ctgaaatgct tgcccaagat ttagaaacaa aaccttttta
     5161 tgagaacgtg gaagataatg aagtattgcc gctattttat tctaatccag aaaagtatac
     5221 ttttttatta cagattttct ttttgaacaa acgctttttg gcaatcaaag atgctttcag
     5281 ccatgatgac aacgtactag accgctccat ctacgaagat agtatgctgt ttcatttgaa
     5341 tgcagattta gggcgtgttt ctgaagtaga agtaaagcag tatgaaggtc ttttagaaac
     5401 gatgctgaag gagttagaag aaatttcgcc acaaaagaaa ccagatctac tggtttatat
     5461 tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa agaggccggg aatatgaaca
     5521 gctagaacaa gatcctgaac tctattccta ctataaagaa ctcaatcgac gatacgaaga
     5581 gtggtatgaa cagtttgata tatgtcccaa aatagtgatc gatggggaca aatatgattt
     5641 cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa atcaagatgc gggcaaagaa
     5701 aatgacagaa gaggcagatg cccagtcgat ccgctacact acaaataacc cgaaagcaaa
     5761 gggaatcttt acaaataaat aatataaaaa ctaagcaaat tgctcactcg cagatatttc
     5821 tctgtgagca gcatcttgct tagttttttt gattcattga gatcaaatag tgaaccgatt
     5881 cgtatgataa aattgccata aatgaaatgt ctgagtgagg gccatgatca aaaataaaat
     5941 ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga cagaaaacaa ggacaatagc
     6001 tcctgcgata aaggcaagaa cgacacttag atagtggaaa aatcgatcaa aatctgcttt
     6061 ttctttagaa atacaataat catataagtt cgttgctacc ccttttaaat ttccagttgt
     6121 aaataaatta gtgtaggcac gcccgttgat tttatcaaaa gctacccact ggatcgatgc
     6181 agtaaaagca atcaaaatcg ttactaatgc gggatgctca tggacgggtg tcaaactaac
     6241 aaagaaaaaa atcactgctt cataatatag aacaaacaag cgccagaaat gtttttcttt
     6301 ttttctaaaa taatcaatta gatactttgt tagaagaatc cctaagaaaa agaaaagggt
     6361 agacaatact tttttaccaa cacttagcca ttctccgtca aatgcttgga tgatcgatag
     6421 aatgatattg ccagtttgag cagaggcaaa agcaccatat tggatataag tataactgtc
     6481 catggctcct ccgataaatg tcagcaaaag tcctattgta cgcccctcgt gaaaggaagt
     6541 ctgcattttt ttcatcacct tttatgaata tttttgtctt ccttatctta gcataaacga
     6601 ttgaaaaaaa ggaaaaattt tcagaaaact aaaatcatat cgcttcccat tttcattttt
     6661 agaaaggtct gttagtataa agaaaaaata gacaacgaaa ctttaaataa ccaacttgaa
     6721 ataaacaaat tcaaagaaga aagatgggaa ggaaatcaat cagttgaatg aaaaagaaat
     6781 tctccgatta caaaaagaat tgaaacgctg ggagaacaag tataaaaagc ttcaacaagt
     6841 ctttcggcta caagaagaaa tcattgctac ctatgaagcg gcttatggcg aagcacccgg
     6901 acttattgag gaagaagtac aagtaaaaga aagtgaagga aacagctctt tctttaaaat
     6961 gatggagaaa cgttcctata atcgcttcaa tcaagaacaa aaagcagcta tcacatatga
     7021 tatgaagaaa catcttcgaa tcatagcagg agctgggagc ggcaaaacgc aaacaatctg
     7081 tgcaaaagct gtttatctga tgacgcaaaa acaagtagac gaagaacgca tactaatgat
     7141 cacttttaca aggaacgctg caaatgaact aaaaaaacga gtagataatt tcagccagag
     7201 aaaaaccgct gtccatatcg gaacattcca tagtatcttt tttaggatat acaatgaaat
     7261 ttgtcgaaaa tttcctgaag tagcaatgca aggaatacaa ggagactttt caaaggatac
     7321 tgcacaaaaa gtgaatgctg ttttgcaaca gctgatacgt aaatataatc tttatatatt
     7381 tgatcaatat ggagaaaaaa cgattgcttc tcggttagac tattggcaga atatgaacct
     7441 ttcagtagaa gaaatggttc agttagttaa agaaaaatat gattccattg ataagaatgc
     7501 acgacagccg atatcagaac gactgtatgg attgctcacg gaactccaag aacaaaaaag
     7561 aagccagcaa ttgctagagt tcaatgatgt actccaaaat ctgaaaagcg ctttggagaa
     7621 tgaggagatc cagcattata tcggccaaaa atatgattat ctattcattg atgaatttca
     7681 agatactaat ccacttcaat gggaaatcgt gaaacggatg acaaaggaaa acaggatcaa
     7741 gttgattgtt gttggagatg atgatcagag tatctatgga ttcagaggat cagagccagc
     7801 atacatcaag aactttgaaa aagaatatcc taccaaaact cttttcttat tgactaatta
     7861 tcgttctcgg gcagctattg tccaaggagc aaatagattg atcagctata ataaaaatga
     7921 tcggatacca aaggcgatga ttccagcaca aaaagaagca ggtgttatgg aagcctattt
     7981 gttttctgat actaaagcag aaagtcagtg gctaatcaaa caaattcaga catttattca
     8041 aaaaaatggg aaatataagg aatcaatcat attataccga tcagcttcac aaacgcagca
     8101 gcttgtccaa tctctgctga aaacgaatat tccttttgta ctggaagcag atagtccata
     8161 tgaaggtatc tttggtatca agtctttcca acatttctat caaaaaattg ttcattggca
     8221 aacagcactg aatctgcaaa agaaaaatca ggcctaccag caattgctcc ggcagctgat
     8281 ggcagactgc tacctgaaaa aaagtgaatg cgatcaatac ttttctccta aatatcaaaa
     8341 tatagccgtt gcagattata ttttgtcgat aagccccaat ttaaaatcga aatctgcaga
     8401 aatccgagag tttgaggaag cgttgcgtaa aacagaccag catcaagaaa tctattcgct
     8461 tgttcaaagc tatcttcatc tgccacgtat agctaaggaa atcgaccaga gtgatcaaga
     8521 atggatacga gaagaagtaa agcagcatgc tactttcaga agactccaag ccttatcgca
     8581 agaaacccaa gcgacagcta aagaattaaa aacccgcttg attgcttaca gacaaggaaa
     8641 acttgatgca ctatgtatcc agagcattca caaaagtaaa ggtcttagtt accgaaatgt
     8701 ctttttgatt ggttgtaacg aaggaagttt gccttataac ggagcgacag aaaagaaagt
     8761 aatcgacttt aatgaaataa aagcagaacc agtgacgacg atagaagaag aacgaagact
     8821 attttatgtt gcgatgacta gagcaagaaa tcgtctgtat ctatgtgtac ctcaaatgaa
     8881 aaattcaaga aaattgaaag tatcgagatt tatcaaagaa accgggagtc gtgtgaaaaa
     8941 aggaaagtga tagaagagag ggtgtgacag aagtggtcag tttcaagaaa gaagaaggaa
     9001 tgtacgaaaa ttgcttctca aatttttgtg aaatttcagc ttattttcga agaagctatt
     9061 tctggaacac agtttattcg gaaaagggag gtgtgacaag aatttt
//
LOCUS       DAJSAW010000083         8954 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJSAW010000083 DAJSAW010000000
VERSION     DAJSAW010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.084"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(162..341)
                     /locus_tag="ONT64_002491"
     CDS             complement(162..341)
                     /locus_tag="ONT64_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303880.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(722..1429)
                     /locus_tag="ONT64_002492"
     CDS             complement(722..1429)
                     /locus_tag="ONT64_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303881.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1422..1859)
                     /locus_tag="ONT64_002493"
     CDS             complement(1422..1859)
                     /locus_tag="ONT64_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303882.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1874..2125)
                     /locus_tag="ONT64_002494"
     CDS             complement(1874..2125)
                     /locus_tag="ONT64_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303883.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2125..2331)
                     /locus_tag="ONT64_002495"
     CDS             complement(2125..2331)
                     /locus_tag="ONT64_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HCR2303884.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2768..3028)
                     /locus_tag="ONT64_002496"
     CDS             complement(2768..3028)
                     /locus_tag="ONT64_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303885.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            3103..3306
                     /locus_tag="ONT64_002497"
     CDS             3103..3306
                     /locus_tag="ONT64_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCR2303886.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3447..3932)
                     /gene="ssb"
                     /locus_tag="ONT64_002498"
     CDS             complement(3447..3932)
                     /gene="ssb"
                     /locus_tag="ONT64_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR2303887.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3935..4834)
                     /locus_tag="ONT64_002499"
     CDS             complement(3935..4834)
                     /locus_tag="ONT64_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCR2303888.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4847..5440)
                     /locus_tag="ONT64_002500"
     CDS             complement(4847..5440)
                     /locus_tag="ONT64_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCR2303889.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5516..5665)
                     /locus_tag="ONT64_002501"
     CDS             complement(5516..5665)
                     /locus_tag="ONT64_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303890.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5681..7885)
                     /locus_tag="ONT64_002502"
     CDS             complement(5681..7885)
                     /locus_tag="ONT64_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HCR2303891.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7896..8918)
                     /locus_tag="ONT64_002503"
     CDS             complement(7896..8918)
                     /locus_tag="ONT64_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR2303892.1"
                     /translation="MIAELKPKHERQNFLVRDDRLDHAVAFLWKDPQTKETVGASYQG
                     TFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLN
                     DTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGA
                     VDPFTNQKVRCERGIANDWQVPREYKVIYEEVAKEEKVTPEAIMAIHKTENNLQLTNQ
                     LVSAHKVNASFGQQLSVNDSIEVISLKDICREVAKELKDCERSNGTYDFDRFYQGKGD
                     INSQILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIHQQEIRKQKRAMLFQQG
                     RQQERE"
BASE COUNT         2298 a         1851 c         1539 g         3266 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaaagtgcta tcacttttag tttttaggat aaaaagtgac gtcacttttt gagtgctggt
      181 acctcaaatc ctgctttttc ttgggcaatc aataaatcta acgcaataca cataatcctg
      241 gattcacttt tgccgtactt ctgctttagt tgctgcaatt ctttttgtct cttttcatcc
      301 aacgtcacgg gtaaccgttt atttcctatt gcaattggca ttagtactct ctcctttcat
      361 cgaaataacc ctattaaaaa tatggctgca atcaacttga atattagact cacaagggtt
      421 aacacaaagc gccaaaagat caaacaaaat accagtattc ctaagaatac tacgaagttt
      481 aaatgtagag ctgcaccatg acgcataagt atctcgccaa caaacagtac gaacaataga
      541 ggaatgattt tattcatatg gatctccttc tttctcttag gttatcgggc tttgcccgct
      601 tatttcgcac cacctaagcg gcgggggcgg ttggatcgat tggtgcgaaa taagaaccat
      661 tcccttaatt tcaaggtttt ttgttcttat ttcacacatc ctcacttttc ggtataaatg
      721 gttattgacc ttttttcctt tcaaatcgtt gatatggcat tcgtttacgg tcaagttcat
      781 tttggatttc ttctgtatcg tcgtaagtgt aggtgtctaa aaaatcagtt aaagtcgtgt
      841 accagtcgtc gcctgggtta tctacttctt ctacatattc cctcaaaaat gcttcgggta
      901 cagtgagttc ataatccatt cccattaaca cgatagattc cgtcccacac gtcaaaaatc
      961 tagtaaatac aaggtaataa tcgtctaccg tcgcatcttt gctgtttagc tcttccattt
     1021 cttcttccat taattccatg ctctcaaaaa ttgaataata ttctgattct gagtaatagt
     1081 gtttcctgca aatcgtgtag ctgacaaagt tttcgaccag ctcactcctt tctaggtaaa
     1141 cattcatgat atatttttct gtttctagtc gtagaagctc cgctcctttt tttaatgtgt
     1201 caggacgacg tttatacacg tctaaacgat tatggctgac tgtaaccttc gtttgactcg
     1261 tttcactttc catatacaac tgttcttcca ctctttgaat aaagtacttc atttcttcca
     1321 tacaactaaa gtctttttga atccgtctga ttacttcatt caagtgttct ttgtttgtta
     1381 aaaattgcga aacatcttct gttgttagct gataaaccac cttattcatt atctttctcc
     1441 tccgattcaa tcgtataaaa tgcaccattt ttttcgatat tttcaaaagt atctagccag
     1501 tcgctagcca ttgctttagc tttcttaatc tgtgcgaatt ttgcataagt cagttctaag
     1561 gcttgatatt cggaaggttt gatagtgctt ggtttgacac ctacttccaa gcatagtaag
     1621 gcttggaaaa accattttcc attttgtttt ttcagttctt ccagtgttcg atagtacgtc
     1681 gcaatgagat tcatttcgac acctgattca atcatttctt ccactgcgac tgacaatttt
     1741 tccttgtaat gttttttggg tacacgtgtt tcatggtata ggaacgtcat atcctccatt
     1801 ttttgtgcca ttttatccaa aggtaatttg acaaattcgt catagtaatt ttgagccata
     1861 ttttttctcc tttttagttc tctaatttgg ttaaccaata ttcgatttca taagtttcca
     1921 tgttttcttg ataatgcagt tcatatcctg caatcttcac taggtcttca aatagcatag
     1981 tcggtgcatc ctctactaaa ggggtttcta tcgtccagta ccgcggaata agtaaagctt
     2041 ctgctacttt ttcagaaatg acttctttgt ccaccaaaat ttgaacgact tcttttacag
     2101 tcaataattt gtgttgtgtt ttcatcaccg tttctttact ttctcatcaa tcaaaacata
     2161 atcgccataa gacaaggtat attttttccc gcatgttgga cattgataca aacgatagct
     2221 tttgatacat ttcgctcttt ctccacaatc gcattcgaca gtatgtggtg catgatagtt
     2281 tgggaataat tcgctgaagg gaacacccca ataatcaaat ttctttcgca tgattgattc
     2341 tgcctcctct taggaaaggt tttatttcct ttcgttagtt ctttctttat tgatttgttg
     2401 gccagaatga atcaacgaaa agcgacaagg acaagggcgc gcagcgtgcc atttaccctt
     2461 gtccttgtcg ctttttggtg ataaaaggca acttgttgcc ggtctggcaa atcaataaag
     2521 agatagcgaa caggaacgaa ccttttggtg agaggaagtt ttttctattt tgtcttgctt
     2581 tgcaagacac catatcttag tgaacgtgca acgttcactt cctttcttct cgaacggcgg
     2641 tggcggttgg agtgagagaa aggtttttca attgagaagg cacttgtgtc ttcctaaaaa
     2701 ggtcttttcc tgtttctatg taaaacaaaa agagctgcaa tcctcattta ggactgcagc
     2761 tcttctttta tatgcccatc gatcgcgtaa aatgatcggg gtttctaatt tgcatcgatt
     2821 caatgactaa ttgattccgt ttattgaaat gacctgtgac ggcaatattg tattttccat
     2881 catccataat taacaattta tcgataattt caatatctgc aactacacaa ttaatcgatt
     2941 catgaattgt ttgtaaagta aagcgaacca ataatggttg tgtttttatt actcggattt
     3001 tatcaagcaa accaacatat ttgttcatca cgatcacctc gctaaaatta tagcaaaaac
     3061 agctaaaatt gagaattttt attcgatttt agatatgcta taatgatcaa gacagtaaag
     3121 tttgtgacgg tggctatccg attgaaagtg aggtggtgcg tatgtgcgta cttccaaaat
     3181 cttacagaaa ggagaagcct tttgcctgtt gaaacggcgc taggattaat gattagtttc
     3241 ggttctttga tcgcaacaat catttttgga atcttagcag ttgtaaacaa caatcaaaaa
     3301 aaataaccgt cagctttagc cggtcgacga ttatttttca aaaataatat taagctggtc
     3361 accgtcttaa acggtgctgt taggggagtt gttagcgcaa ctcctctttt cgtatccatt
     3421 ataacaactt tattgatctt ttacaatcag aatggtaaat catcatcact aatatcgatt
     3481 gattgtccgc caaatggatt ttgtgaagcg ttcaatccat tgttgttagg cgtagctaca
     3541 tcccgttgtt caaaggtggt agggcttgga ctgctggacg attgatcagc gtgcgcacgg
     3601 ctttctgtcg ctgctttact ttctaatagt tggaaattat cggccacaac ttctgtcaca
     3661 tacacacgtt gtccttgttg gttatcgtag gttcgggttt gaatccgtcc aacaacgccg
     3721 atcaaactgc cctttttagc taatgtcgcc atcgtttctg ctggtttgcg ccaaatcaca
     3781 cagttaataa aatcagcttc tcgattgccg ttttgattcg tgaaatttcg gttgacagct
     3841 agggtaaaag tcgctactgc agtaccactc gcggtataac gtaaatctgg atctttggtt
     3901 aagcgtccta ctaaagttac attattgatc atctttatct ctccatttct tgattcattt
     3961 gttgtgatct tttaaactgc cgttttcttt cttgattttc aattttgatc acacttttca
     4021 ttaggtgatc aatttcgttg actgtatctg taaatgaaat ggaattcgca aacgtgttaa
     4081 ctgagcgata tttttcccac tgcttttcca aatagtcatt tcctctgatc ttatccattt
     4141 ctctttcata ataaaaggac aacggttttg tatccccacg ttttgtaaat gtatttcgaa
     4201 cacttttgta taactctttc gtatcgatat tttctagttt cggaatggta aataagtcgt
     4261 agtaatcttt tgctctcgaa ttatctgtgc caaagctgaa cgtagcactt agcttttctg
     4321 caataatttg ttctgttgga taagctggaa tctccacttt tttatcttca aacattaatt
     4381 ggtgatagtg tgtatctact tcaggataaa tagaatctcc tgttgtcacg tccattttaa
     4441 acggtacacg tactttctct aaattagcca ccaatttaat ttgaaatcca ggataaaaat
     4501 cggcttctct tgtttccttc attccttgaa tagagaaaac aatgccttct tttgtgggtt
     4561 ttctagttaa atcattaaaa atgctcttca atttttcttc agttaatttg ctatttctaa
     4621 atgtcgtatc gatatcaatt gttgtccttc tatcgattcc atattttgat cctaataaaa
     4681 agccgccttt caaaatgaag tcatttttgt actttgattc agaaatttgt tcaattagac
     4741 gatccatcat aaaatgccgt tgcactaact gtgcatttaa tcctgtactc tttgctaaat
     4801 ttcttgtttt tgctgcaaat tgtgtcggtg tcatcttttt cctccatcaa tataagcttt
     4861 gcatgtggta tttcatttct ttttctacat gtagttttgt catgtaatct cttaatttac
     4921 ttggatctct caatgggtct tccatgtact cttttaatgc ttctaatttc atttcatatt
     4981 caaattcata tctcggattc caaatatcac aaagtgtacg ctctttatca tacatagtaa
     5041 taggatttcc ttgaaaggaa gttccctcta cttttcctag ttcataaact tctttcttca
     5101 cgaaatgaaa ttgtaactgc tgctcttttg taattgaacg atcaacgtta tagccatggg
     5161 gaaaagttaa gtgaatgaat ttcggcatat tcgtagaaag ctggtaaata tctaaagtcg
     5221 tttcatgaga gaagattccc ctggaaaatc gttgctgaag ccaaaataaa tcatctattc
     5281 catgaccatc ctctacataa taaccacgat caactttgat caattttcct tgattcacca
     5341 atctttgtac agtggttgct gcgattccaa atttcctggc ttccttcata gataaatttc
     5401 catcgcgaat agccaacaat tctttcagtt tatcttgcat ttacttcccc tcccttcgtt
     5461 acaaatacac tacatcaaat atatcatagt agtttatttg taacaagatt aacgtctatc
     5521 gatctaattc gatttctttt tgaatgcgct gctggttctt taattgttgc agtcttcttt
     5581 tatttgcacg taatttctct tgttctaaac gaatttttgc tttttgattt tcgatttctt
     5641 gttgtttgtg tgttgtattt cgcataggat aaccccttca ttattttgta gtgcggcgtg
     5701 ttcttttctt atagttcgtt tttttctttt cagaaaattc aaagcctgtt gtaaagtctt
     5761 cttttaaaac gatcgttgct tcaaacgaac ctttctgacc ttttagtcct ctgactgatt
     5821 ggcttgttcg gccgttttga atgatctctt tcagttgtgt ggctgtgagt ttctttttgg
     5881 caatcgttgt ccataattta aaatcacagg cttcagacgt acatgccgcc acattttgat
     5941 aaagcaagac ttcttgttcc cgacattttg gacaaacacc taaatgacgc gttgcttcct
     6001 gtttttcact ggctgctttc acctcttgta acgttgatcc aaaatctgtt gactggattt
     6061 gtcccggtac gacttccaac aagtgagaga caaatttttg gatattagct aagaaagtag
     6121 cttgtgttcc tttctgctca ccaatcttta ataagtaact ttcccatttg gcggtcattt
     6181 cggcacttgt tagcagcggt tctttttcaa cagcctgaca aagcaaaatt cctttattcg
     6241 tcaccacaag tttatttttc tctaccttga tatattcctt ttgcttcaag gtttcaataa
     6301 tatttgctcg tgtcgcttct gtaccaattc cctccacttc tttcaatatg gattgtgctt
     6361 cttcactatc cacggtttta ccagctgtct tcatggcggt gattagtgta ccttccgtgt
     6421 aaggttgtgg tggcttggtt tctttttcaa agatttgtag atctaccgtc accgtttctc
     6481 cttttgttac tttaggaagt agcggttctt cttccttctc ttttctgtcg ctctctttgc
     6541 caaatagctt tttccaacct agatctactg gtacttttcc gactgccttt agctgaagct
     6601 gctggacttg tgtcaaaatc gtagtttctt cataggtata tttttccgca aacatggcca
     6661 ccgtcgtctt taaaacttga tcataaatcg cttgttgtaa aggtgtcagc ttttcatacg
     6721 ttgcttctgt cataactgtt tttgtaggaa tgatcgcgta atgttcctgg actttcttat
     6781 tattcacata ccgactattc ggtgtatgaa taggagtagg gaatgattca gcctttaaaa
     6841 aatgcttgta ttcatctaaa tgatccaaca gatacgcatg ctctccttct gtaatataag
     6901 gggtgtctgt tcgtggatag cttagatatt tcccttcata caacccttgc atggcttcca
     6961 aagtatcttt ggcactagct ttcatgagct gattcatttt agactgaagg ctggatagag
     7021 aaaaaagacg tggacttgcc gcgctttttt tctcggtttc gacttcaaga atcgtgcctg
     7081 gctgctttcc tacttgaaca cctttttctt ttaaataggc ttcaaatgct tcttgcgtcg
     7141 cttgggtttc attgggatca agcttcgcat caaatatgcc attttgagcg gttacttggg
     7201 ctttcccttc aaaataaggc tctttttgga agtttctaat cttttcctgc agctgataaa
     7261 tcatgtaaag cgttggggtt tggactctgc ctaaggaaaa acttcctggt atgccctttt
     7321 gctgcaagag taaactatac agtgggctgc cgttcatgcc gatcaaccaa tcagcgatct
     7381 ggcgtgcttg tgcttcttca aatttgggat aggtctcttc tgcgtctcgt aattgctgga
     7441 atccctgata aatggcttct ttttctaagg agttgatcca aagtctttta aaggtttttc
     7501 cttgatcagc gcctgcttgt tgaatgatcg tccaagcgat cagttcccct tcacgatcgc
     7561 tatctgtcgc aataataatg gtgtcggccg attgaagttg ctgtttcacc acgttgaatt
     7621 gctttttctt atctttagga acatgatagt gataatgtgt agggaaaata ggcaaatgct
     7681 ctaatgacca ttgtttccaa ttttcatcgt acatatcggg cgaatctaac tcgactaaat
     7741 gaccaaaacc atacgtgatc acagtttcac ccgaaaacaa tcgatcttgg atttcaaaat
     7801 acccgtcttt tcgggtggct ttagaaaagc tatcggcata ggccttcgct tgtgacggtt
     7861 tttctgctaa aatgacagtt ttcatcgtga cgatctcact ccctttcttg ttgtctgcct
     7921 tgttgaaaca acatcgcacg tttttgcttt ctaatttcct gttgatggat ctcatgcttc
     7981 aattgatcgt tccaatcctt tttcacttca ggtacaaatt catgttcatg atttttatag
     8041 cctttgtagt attgttctgc tttataagag aaaagaatct gagaattaat atcccctttt
     8101 ccttgataaa aacgatcaaa atcatacgta ccattcgagc gttcacaatc ttttaattct
     8161 tttgcgactt ctctacaaat atctttcaaa ctaatgactt ctattgaatc gttaacagaa
     8221 agctgctgac caaaagacgc attgactttg tgagcggaaa ccagttggtt cgttaattgc
     8281 agattgtttt ctgtcttgtg tatggccata atagcttctg gtgtgacctt ttcttctttt
     8341 gcgacttctt cataaatgac tttgtattct cttggcactt gccaatcatt agcgattcct
     8401 cgttcacaac gtactttctg attcgtaaat ggatcaactg cgcccattaa ttgttctttt
     8461 tcgtagaaaa tatgtcctgc acggtcatta tccacacaaa tctcgatgga ttgaggaaag
     8521 gcttgtttct ttctcaattc agatactgcc tcaccaaaat aatgactgat cacatggtgt
     8581 tttaaccctt ccatagagac taaccacgta tcgtttaact gatcacgatt cagtgcagcg
     8641 tagctaagta aatcaatact actctcaaaa aattttaact gtttaggatc accaatcttt
     8701 aggttaaagc catgattcgc tgtcgaattt ttatctatat gcttatatgt gcctctctca
     8761 ccaaaacgtt cataatcaat gaacgttcct tgataactcg cccctactgt ctcttttgtc
     8821 tgtggatctt tccataaaaa ggcgaccgca tgatccaaac gatcgtctcg aactaagaaa
     8881 ttttgccgtt catgttttgg ctttagttcg gcaatcaatc cttctttttc tgcataatca
     8941 atcaaccaat cggg
//
LOCUS       DAJSAW010000084         8953 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJSAW010000084 DAJSAW010000000
VERSION     DAJSAW010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.085"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            165..1379
                     /locus_tag="ONT64_002504"
                     /pseudo
     CDS             165..1379
                     /locus_tag="ONT64_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1558..1887)
                     /locus_tag="ONT64_002505"
     CDS             complement(1558..1887)
                     /locus_tag="ONT64_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR2303893.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1877..2149)
                     /locus_tag="ONT64_002506"
     CDS             complement(1877..2149)
                     /locus_tag="ONT64_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR2303894.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2600..3403)
                     /locus_tag="ONT64_002507"
     CDS             complement(2600..3403)
                     /locus_tag="ONT64_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR2303895.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3381..4505)
                     /locus_tag="ONT64_002508"
     CDS             complement(3381..4505)
                     /locus_tag="ONT64_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2303896.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4521..5336)
                     /locus_tag="ONT64_002509"
     CDS             complement(4521..5336)
                     /locus_tag="ONT64_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2303897.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5326..6198)
                     /locus_tag="ONT64_002510"
     CDS             complement(5326..6198)
                     /locus_tag="ONT64_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2303898.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6283..7572)
                     /locus_tag="ONT64_002511"
     CDS             complement(6283..7572)
                     /locus_tag="ONT64_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2303899.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7800..8444)
                     /locus_tag="ONT64_002512"
                     /pseudo
     CDS             complement(7800..8444)
                     /locus_tag="ONT64_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2782 a         1899 c         1426 g         2846 t
ORIGIN      
        1 ctaaatttta ccaaaagtga gtctcttttt gagttgaaac gaagaaaaag tgttagtaga
       61 aaatcgcctt cgattttcca ccaacacaaa atattataga agcaaaatca attctacctt
      121 agatgttctt atatcactct atcagtccta tttgacaaag agccaagaaa aagacgttgg
      181 tcgtcgatgc tgttctctcg cctactcctc gtgcttgtag aagttgtggt tctactgtgg
      241 tagatggaaa cgggaaagca attatagtga aaaatgggaa gaaggaaacg attgtccgtt
      301 ttgaacaata caatcatatg cctttggtta cgcgcctaaa aaagcaacgc tatacctgta
      361 aaaactgtcg tacccattgg acggctcaaa gttattttgt ccgactcagc cattcaatcg
      421 cagatcatgt tcgatataaa attgcttctt tactgactga aaaagtatct ttgtctttta
      481 ttgcgaaaag ttgtcaggta tctttgacta ccgttactcg tacattgaaa gagtttaaaa
      541 gctatttacc aaagccttct aagaagattc tccccagagt attgatggtt gatgaatttc
      601 gttcgcacgc ttccatagaa gataaaatga gctttatttg cgcagatggt gaaacaggaa
      661 acttaattgc tgtcttgcct acgcgtaaat tacctcgatt aacaagctat ttcttaggct
      721 gcaccaatcc agaagaagta gaattcttag tcacagacat gaacgccgct tacttccagc
      781 ttaccaaacg tgttctgcca aatgcgaaag tagtgattga tcggtttcat attgtcaaac
      841 acatgaatca ggcactcaat gagttgcgta tccgtgaaat gaatgagctt cgtaaagcag
      901 gacagaaaag tcaggcagaa aaactgaaaa agaactggcg ctttctgcta aaaaatcgtg
      961 caagcatcaa ccattatgag tacaaaacat ggaaaagttt ccgagcacca aaatacccat
     1021 ttctcactga agcaatgatg attgatcgat tgcttgagtt ttctgcgcct ctaaaggagg
     1081 cgtatccctt ttttcatgaa ttagtcgaag cctttcgaga caaagaccct gacttatttt
     1141 tctccttatt ggcagaactt cccgaaacgt tagatgacgg ctttcgggag aagcttcaaa
     1201 accttctgac ctatgaagaa ggtatcacca gcgcaatgat ctatccttat tccaatggaa
     1261 aaattgaagc gaagaatacc cacataaaga caatgaaacg agtatcctac ggatttaaat
     1321 cattcgagaa catgagaatt agaatctttt tgatcaatca attaatcaat gtaagataac
     1381 aaaaaatctg aaccagaatg tggttcattc tgacccggat ttgattttgc acaactcatc
     1441 agtccttatt gacaaagagc caaaaatctg agccagaatg aggttcattc tgacccagat
     1501 ttgattttgt acaacccatc agtccttatt gacaaagagc ccttcttttt agtattatta
     1561 taattcttcc atgcgttctt ccagtcgctg taaatcgctt ggacttaatt ccccaatttt
     1621 tttccatttg acctttttgt tgtttttggg taattcgttt agagttccta cgtcaatcca
     1681 agattctttt tttagtcctg cctgtttcca atcccttatt tcaacgtatt tcagctttat
     1741 tttatcggat ttagtatgat attttgaagt aattgacaga aacgaaattg tgtccagtcc
     1801 attttttaga atatagacag gccggcgttt accgccctct gcagctgtga aagcaacgta
     1861 gacggttgct atatcattag tattcatctt cattgaacca ttcttcaatt ttttcgtcag
     1921 tgtctagttt ttggacaggc aatttttccg ctaatctttg aatattatgg attcgtttac
     1981 ttttttccga caattcaata gaaaatggga tttttccttg ctcaacaatt tgcttataaa
     2041 aaacggtgac ggctgttttg gggctcatgc ctagttcatc aataatgctt tctgcttcat
     2101 ttttcaattt ttcgtccata gaaatttgta acttagccat tttctccacc tctttctgac
     2161 aaacacatta tatcacggaa agagttcaaa tggaattaat acaactactt ttgaactcct
     2221 tgaaatgcag tgtatttatc taaatataag gaaaggatat agtgctaaat ggccttgata
     2281 gatagaaaag tttcaaaatc cgccacagcg ataattgaag cgaacactca acgacacaca
     2341 gaacaggaaa agactcctaa aagaaccaaa ttaactagga cgtgtctgaa aactccacaa
     2401 agatgtggta tgctctggta aaaaacgaat ggcacaacca cgaagaccga tatggttctt
     2461 ggaaaacttt ctccaccctt tttgtaattg gagggatagt ggtttattga tagcgatttt
     2521 ccaagttctt caagtggaac cagacattga gcaccacggc taaaaagtga atgaagtctt
     2581 tatttttaat caataatttt taatgattac cagtaattgg ttttttcatg cgccctattt
     2641 ctatttgatg tggagttaaa taatgctgat aagtaaaaat ttttccgtca attaaactgc
     2701 cactagtgaa tcctgttcta ttggtaaaaa gagaatagtc tgggtattca tctacaccaa
     2761 atgataaaga ttcagccata aactgttttt taccagcaaa ttcagcactg ctaccttgat
     2821 ggatatgtcc tacaaaaata cctaaaatat cactttcggc aatcaattca gcaaaacctt
     2881 ccggaacttc agttacaata gatggatctt cccacgacaa tggatgatga accaataaaa
     2941 tcgttccctt ttcagcaggt gtacccaacc actcttgtaa ttgaatcatc tgtcctatcg
     3001 atatttttcc ttcgtgctgg gtatcttgag cagaatccaa ggaaataatt cgtaacccat
     3061 tttgattcgt acaatggata tactctcccg tttcattgct ttcaacactc attccttcaa
     3121 agaaaacact gcgacgatca tgattcccac gacaaaaata gtaaggtgtg gttgggaaat
     3181 attgatccaa cattttccgt aagacttgat aatcagaaat ccccccttca tgtactaaat
     3241 cacctgttaa tagaataaaa tcgtacactg ttaacgagag ttcttgtaac aatttttcca
     3301 atgccaactg tggattttta attgttggaa atagttcagc agtaaacaag ttagtttgat
     3361 agttttctct aaaatggata tcagagagat gcaaaaagcg catggctatt ccctctttct
     3421 tcaataaaat aatcaggata tcctaaattt tcttgcgtct gataatcaaa aaaatgcatt
     3481 ctttcaggtt ctatgcggag attgactgca tcaccaatct gccaagtagg tttttcatta
     3541 aggacaatca tttctatacc atcgacttta acggtgtacg cataactttg acctaatagc
     3601 tcacgatact tcacttgacc gtgtaaatcg cttgtttctt ttgaaaaggt tacgtgttct
     3661 ggtcttatgc ctaaatacaa tgagccacta acctgaagaa gttgtttttg ccaagctgga
     3721 ttcaatgtta aaaattgact ggctaccttt aatgttcctt gatcataagt aactggcata
     3781 atattcatgc ctggcgaacc aataaaattt gcagtaaaag tatttttagg tttcgtataa
     3841 acatttttcg gcgtatcaat catctgaatt ctaccttcat tcaataaaac aatccggtca
     3901 gctaacgtca tcgcttcaat ttgatcatga gtgacgtaga caaaagtttg tttataacgt
     3961 tgatgaattt ttaccaattc ttttcgtgca tccaatcgta aacgcacatc caaatttgat
     4021 aacggttcat ctaataaaaa ataattactc cgtttaacca ccgcacgagc caatgctaca
     4081 cgctgccgtt gccctcctga caaatcttta ggaagtcgat cttggtatgg cgttaaacct
     4141 aacatcttca aagcttcttc caaacggtgc tgaatgtcag ctgtcttaat tttattcgcc
     4201 cttaatgcaa aaataatatt ctcctttaca gtcatgtgag gatataaagc ataattttga
     4261 aaaaccattg cgatctctcg ttctccacaa ggtagcttat ttgctagttg tgtacctaaa
     4321 aaaagctctc cactcgtaat tttttccaaa ccagcaatca ttctcagcaa agtagatttt
     4381 cctgaaccag atggtcccag taacacaatt cgttctccag cggccacagc taaatcaatt
     4441 tcttttaaaa tggtatgttc tccatatttt tttccgatat ttcttaattc aagcgtttct
     4501 cccatgattt gtcctccaat ttatttaata cctgcagaaa caaaagtccc gataatatat
     4561 ttttgaaaaa ttgcatacaa gactaacggt acaaacattg taattaaact aatcgccatt
     4621 gctacggtaa aatttgtccc accttcagaa ctaatatagg tctgcatcgc caacggcaaa
     4681 gtataattat ctactgtccg taaaattaat accggccaaa catactcatt ccaagaggta
     4741 ataaaaaacc aaataccggt agaagtaatc gcatgtctag taagaggaaa aacaatttta
     4801 ctcatgacgg ttgtttgttt gatgttatcc aatgccgcca catctagaag gctcactgga
     4861 atttcccgca ttgtttgcgt caataaaaaa atgcccatcg catcagcaaa ttgtggtatt
     4921 atcacacccc aagtagtatc aattaaatta agttttgcaa tcaacaaata attaggaatc
     4981 atcgcaacag taaaaggaat gaacatcgta ccaatgatga taaagtacca gattttcttc
     5041 cctttgattc ggcaataagt aaaactatat gccgcaaaaa aagctacgcc taacttaaca
     5101 accgttataa ttgtagcaat aaaaaaagta tttctcgtaa ttttgataat tggaagattt
     5161 tcgaacaaat agcaaaaatt ttccaaggta aacggctgtg gtatcaaact agtaatcgtc
     5221 tgataggcat ctttcaatgg cttaaaagaa ttggccataa taaacaataa tggatagata
     5281 ataatgatta tgatgaacat aaataagaga tgtggcaacc agcgtttaat tttcatagta
     5341 aacccccttt tccacaaatt taaatattac ccctaagaaa acagcaaata ttaataaagt
     5401 taaaactgaa attgctgaag attctccgac tttatacatt tcaaaagcct tttgataagc
     5461 ataatagatc acgtttgcac taccattatt cggtccacca cgagtgatga tcttaatcgg
     5521 agtaaacaca tactgcagac cttgaacaat ggtcatcacc aataaatata gtcctgtcga
     5581 actactcata ggcaacacaa ttgtaaaaaa aatgcgccag atgggtacac gatccaactt
     5641 agctgcttcg atcatctctt gatcaattcc cacaattgct gcaaaaatga caatgaaatt
     5701 ataaccaaaa actttccaag cagtaatacc tgcaatcaca gcaattaccc aaccttgtaa
     5761 attcatccac ataggcagtc ttaaaccaaa atttcccaac aagacagcaa ttggtcccga
     5821 taccggattt aatatccatg taaaaaccat ccccgccact accaaagaaa tcacagctgg
     5881 aataaaaaat aatggtttat acaaattctg ccctttttct ataactattc ctaataaaaa
     5941 ggatgtaata tatgggaaca cacagctcag caagacaaat agaacggtat aggacaaagt
     6001 attcttcaac actttatgaa agactggatc agtcagcacc tttacatagt tggcaaagcc
     6061 aacaaattct ttagtggggg ccaccatatt ccagtcaaaa aaactcaaat agatcgtata
     6121 aatcaatgga taaaaaacaa acacgcccaa caaaataatt gctggcgcca gatacaaata
     6181 acctacatag cgcttcattt ccgacagcct cctcatcctt atgtcaaaag cagacgacct
     6241 tgtttttcct ttcttacagg cgctgtcgtc tgcttgcttt gcttatttga gtaactcatt
     6301 aatttccttt tgagtagctt tcatcgtttc attgacggaa ttacctgata aaattttatc
     6361 ccgcatatcc aacaattttt gttcagcttc taatccagca tctccaggaa atgaactcca
     6421 actgaccatc ttatccatct gatcaattgc tggtatcatc atctgatttt cagctaagaa
     6481 agcttttaat ccattttggg cgtctgcaac atctttacgt ggtggtaaat aaccagtacc
     6541 ttctgtccat ttagctaaag attcaacact ataaagatat tttaagaaat cccaagttgc
     6601 ttcctttttc ttatccgttt gtgatgtaac agctaacatt gcgccaccag caggtagttt
     6661 aacttttttc ccttcccaac ttggtgactc aactgcttta gcagtaaaag tactattttt
     6721 ttgaatgttg tccctttgag caatagtggt atagaccatc gcgacgttac catcaataaa
     6781 gctttgcaca ccttgttccc acgtagtgtg taatgccgtc tggtctttta ccatcctttg
     6841 atataattca taagcagcga taccatcttt agaagcaaag gtagctttgc catcttttat
     6901 tacacttgcc ccattacttt ctaaaattgc ttgttgtgcc caattatcag caggttcttg
     6961 aatatacacg ccatactttc cagttttttc ttttattatc ttagaaaaag tttgaacttc
     7021 ttcccaagtt tttggaccat tttcatccaa gcccgctgct tttaatatat cttgatttaa
     7081 atataaaacc ggattactaa tggagtaagg aatacccact tgtttaccat ctttttcaga
     7141 cagtgccatg atattgtcta aaaaatgatc tttaaaataa tcagcattat ccggatccac
     7201 ttcttctact aaagattgag gattagtata tttgaaattt tctgcaaaat aatctttaaa
     7261 agcccatccc acttgtacta cgtccggtat ttctttggag gcaacagaag cttgtaaatt
     7321 ttgcatcaag cctttataca tatcaggatt atattttgct acaaccttca ctttgtcttg
     7381 agattgattg tatttatcta ctaattcatt cactgttttt ccacctaggg tatccgcatt
     7441 aacatgccaa tactctatcg ttacacgacc ctccgatttt tttgttgtct tggtattccc
     7501 actaccacaa gctgccaaag agatgacact taaaacagac acaaccccta taacaagatt
     7561 tttctttaac ataacattaa cttcctccta atttctttat ggccattttt ctaatcaaga
     7621 aatattatga actaccaatg taaatttcta gttaattttc tgtaaataat tcgaaatttg
     7681 tgcatttcgg ggtacagggt cactggacat atccatcaag ctaagaataa agcccccata
     7741 tggtcatgta tcccatctcc aaatctctta actcctctgt tttgattata aaatagttta
     7801 ttctgtgctc ctttcttcca taaataggcc catgcacaac gtgaggttat attataaaca
     7861 ccacagcgcg caaggtactc aatcgtttct agttcattga ctgtccatac agaaaatgtc
     7921 aatcctagct gctgccccac ttcacgggcc aacttagtta accatccttc atgaatattg
     7981 acgatttgaa cgctttgata ggctaatttc tgtaaggtat cgatcaaatc tgcttcggta
     8041 atatccccaa gttttttccc ttcgaactga ttttcaatat tataaaatat atgctttccc
     8101 caaatcgcag cccaagataa ttgtacatag ccactaagaa caatttgtgg ccagatttga
     8161 taggtttgtg ccagcgaaaa aacggccatt tccaatccct cttcttttaa atcacagtta
     8221 atggtcatgg ttgccttctc attggccact gcttgaaaca cctgctcaag ggttaccgaa
     8281 tctgggacaa cttcttacct gtcatgggct agtattagcg taccatctgt ttgctgacgc
     8341 acatctactt caaaacaaag ctggcgatcc aaacaaaagc gaagaaatgc ccagctattc
     8401 tcctccgtat tgtcactacc agagtgtgca gtgatcatgg tcattttgac ccctccttta
     8461 attccgtgat tttctcccac acgtccacta taccgatgaa gcgttaattt cttgttaatt
     8521 tcttgtaaat tccctgcgtt ttttcagtaa actagttgta aatcttgcat gctccttcct
     8581 acgaactcat gaatcgtcga ttttgccctt ctagctagtt tcaccaatta agtagttcta
     8641 cattccccgc ttttaaccta ctacaccttt ttttgttttc agctattcct tcttcttgct
     8701 gaaagtcctt gaggttcttt cttacaggat accatcaagg tgttcaatgg cttattccca
     8761 ttgaatgacc gagacttact atcagatttc tgctcgttga accaagtctt tgatacagga
     8821 tttcttccgt catcttctca caaataccac cttgcgtttc tctagcgatt ggtcgatgtg
     8881 caccctccca gtggactgtc actacccctt tttgctatgc ctagcccaca aaaaaagctg
     8941 tcctcacaga cag
//
LOCUS       DAJSAW010000085         8920 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJSAW010000085 DAJSAW010000000
VERSION     DAJSAW010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.086"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..777
                     /gene="menA"
                     /locus_tag="ONT64_002513"
     CDS             <1..777
                     /gene="menA"
                     /locus_tag="ONT64_002513"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR2303900.1"
                     /translation="NYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVI
                     GVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYV
                     NTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDI
                     ENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHL
                     DQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1712..3058)
                     /gene="gor"
                     /locus_tag="ONT64_002514"
     CDS             complement(1712..3058)
                     /gene="gor"
                     /locus_tag="ONT64_002514"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR2303901.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3192..4445)
                     /locus_tag="ONT64_002515"
     CDS             complement(3192..4445)
                     /locus_tag="ONT64_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR2303902.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4451..5374)
                     /locus_tag="ONT64_002516"
     CDS             complement(4451..5374)
                     /locus_tag="ONT64_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR2303903.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5595..8087)
                     /locus_tag="ONT64_002517"
     CDS             complement(5595..8087)
                     /locus_tag="ONT64_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCR2303904.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(8094..8561)
                     /locus_tag="ONT64_002518"
     CDS             complement(8094..8561)
                     /locus_tag="ONT64_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCR2303905.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8697..8770)
                     /locus_tag="ONT64_002519"
     tRNA            complement(8697..8770)
                     /locus_tag="ONT64_002519"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8734..8736),aa:Arg,seq:acg)
     gene            complement(8828..8901)
                     /locus_tag="ONT64_002520"
     tRNA            complement(8828..8901)
                     /locus_tag="ONT64_002520"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8865..8867),aa:Pro,seq:tgg)
BASE COUNT         2479 a         1884 c         1485 g         3072 t
ORIGIN      
        1 aattacatgg attatgaaaa ggctcattct gaagtatata agtatgaaga aaatgtcatt
       61 ggccgcagaa atctatctcc tgtattgatt cgaaacatga ttttagctat gattgcattt
      121 gtttttgtta tcggcgttat tctaacgatc aatacgggtt ggcttttact tttgatggga
      181 tttatctgtt gttttattgg agtattctat acattcggac cgattccttt atctcgaatg
      241 cctttagggg aaattttcag tggggtgact atgggccttg gaatttttgc catggtcgtt
      301 tatgtcaata cattcggttc agatgtctat gatctgacag tcaacttttc atctggacaa
      361 tttgatttgg taggaaatct ttgggctata gcagcaatcg tattagcttc cttacccttg
      421 gtgtttacga ttgccaatat catgttggct aataatttgc gggatcttga ccgtgatatt
      481 gaaaatcatc gctatacact agttttttat atcggacgtc ctgcgggaac agtcttattc
      541 cagctattga tgtatgcttg ctatttcgtg attttaatgg ggttgttgag cggtatctat
      601 gaatggccga tcttggtcac cttcttgacc ttgccaattg tgaagaagca tttggatcaa
      661 ttcaagcaaa cgttacccca gcctatcagt ttcagctatt ccatcaaaaa tatggtctta
      721 ttcaatgcca gttatgtatt ggggctatta ctaacaatca ttatgcaagc catttaacag
      781 catgaagagg ttgtgaaaaa actgccctgc atcaagtaat aagaacagcc aaataaaaat
      841 agctccttat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
      901 gtttatcagg aggttgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa
      961 aatggctttc cacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaaca
     1021 ccgtttatca gaggttgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
     1081 aaatggcttt ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac
     1141 accgtttatc agaggttgta aaatcagtgt tttgtcctga gtattagaga aacaaatgga
     1201 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
     1261 actatttgtt cggaaaaagt gactaactat gacacaattt ttttcacagt cccttttttc
     1321 acttgatttg attcgtacca gcgaacttga cgagaacttt cttggtttgg taactatatg
     1381 gtattctttt aacatcctcg tttgcttctt ctaatttatt tatacaaagt cacacttttt
     1441 taaacatctg ttcttcgata catttctatt tcttttaaac atcgatacca atattttgaa
     1501 cttaagagtg cataccaaat ggattcattt actcttcgtc agttacgaag ataatcaaaa
     1561 tctccaactt ccctacttat ttcgttttga gaattatcga agcaatctta cataaataac
     1621 taaaaaacaa aaaaccagaa aatcagtata ctgattttct ggttttacat ggtttgttca
     1681 ataaaaagct gttggaactg ttaatattaa attatcgcat cgttacgaat tcttccgctc
     1741 ctgttggatg gatggctact gtattgtcaa agtcttcttt agttgcaccc atcttgatag
     1801 ccacggcaaa accttgcagc atctcatcga caccaacacc aatagcatgt aagccgacga
     1861 ttctttcttc ttttccgaca cagatcaatt tcatttcgca tttttggcga taatcattta
     1921 atgcaaagta cattggggta aagcgtgaac gataaacttt caattcattt tcgccatatt
     1981 tttccaatgc ttcttcttct gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga
     2041 ctgtcggaac aagtttatag tcaagataag aatctttctt tccattaaat aaacgttctg
     2101 ataaacgtct accagctgcg attgcaacag gagtcagatc taatttaccg atcacatcac
     2161 caacagcaaa aatatgttca tccgttgtat tttggtattt atctactttg ataaaccctt
     2221 ttttgtccaa ttccacattt gtattttcca gacctaattt ttcggtattc ggtacacgtc
     2281 caccggcaaa aagtacacaa tcaccagtta aggtttcacc attttcaaat agcaccgtat
     2341 attcgtcatt gtttttagta atttctttag caatatgatg tgcgtaagtt tttattccag
     2401 cttcttggta catttggatc aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc
     2461 gttcttttcg atatgcccac atgacttcac tacctaaacc acgaaataca cctgaaagtt
     2521 cagccgcaat gtatccagca cctaaaacga ttgctctctt aggtaactct gttaattcaa
     2581 aaaatccatt tgaatcaatt gcgtattctc caccaggaat ggtcatttta cttgcacggc
     2641 ctccagtagc gatcaagatt ttaggagcac gataggtttc accgtttact tcgatcgttt
     2701 gactatcgat aaaagtagcg taacctttta tagcctcgac atgattgcta tctaatcctc
     2761 tttgataagc accgtgtaga aaatcaatat atttttctcg tctttctact agaccggcga
     2821 aatcaacatt ttttaaatct gcttggatac cgtaacttga ggcatctcgt tcaattgttt
     2881 ccaaaatcgt acttgcttgc cacataactt ttttaggtac acaaccgacg ttgacacatg
     2941 ttccgcccaa ttcattcgct tcaatcagca aaacttttgc gccatgcatc cctgctctgt
     3001 ttgctgaagc aataccgcca gagccgccac caattacgat gtaatcatat atttgcattt
     3061 tgttcctcct tattgaagag atcaatcaaa acctttattt aacgatcctt tttctacaga
     3121 aaacaagtgt aacacttttc ttgaattttg tatgcgaaga tgcttaaaac agggatatat
     3181 cagctttttc cttatttacg ttttcggatc atatctccag gtttcaccgg ctgtgcgact
     3241 ggcatagtta aaatcatcat tggatttggt gctcggtcaa tggattcgcc ttcttcatta
     3301 tgcataactt caacggtttg actgaaatga ctgaatcctg gaccataaaa ttcgatttca
     3361 tctcctacag aaaacaagtt tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa
     3421 gataatactt caccgacaaa tttatatact gggattttac gacgttcacc gaataattgt
     3481 tcgttttcag ttggtgtatt gtagtaaaaa cctgtagcta attctcgttg tgctactttc
     3541 cataactcgt cgatccattc ttgcttgcag acatagtttt caggatcttc catataactg
     3601 tctacagccg ctttatagac atttgacacc gtagatacat aatggatcga tttcattctt
     3661 ccttcgatct tgaagctgtc tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt
     3721 gacatatcca ctgcactcat cgagaattct tcttccactt cgcctgtatc agttaaactg
     3781 tttcgttctt gaccaaaagg catatcgaac aatccatact tccagcgaca agattgagaa
     3841 catccgccac ggtttgcatc ccgcatcgac atatggttgg acaatgtgca tcgaccagaa
     3901 taagagatac acatggcacc atggataaat gcttcgattt cggtatccgt atttttacga
     3961 atctctgcca cttcctccat tgaaacttca cgagctaaaa cgacacgttc caacccttct
     4021 tctttccaga attccaatgt ctcaaaatta gttgctgaag cttgagtgga taaatggatc
     4081 ggcagacctg gggcttctgt cgcacaaatt tcgatcaaag ctggatcaga aacaatcact
     4141 gcagaaatac cgacatcacg caaactgcgg aaaaattccc ctgctccatc ttcatttcct
     4201 tcatgggtga ccatattagc agcaacgtat accttcgcat catgttcttt tgcaaaagca
     4261 actccttcag ccatttcttc ataagtaaaa ttacctgcac ggcttcttaa accatatgca
     4321 ttcccgccaa tatatactgc atctgctcca taatggatcg cggtttttaa cttttccagt
     4381 gttcctgctg gcgctaggat ttctggtcgt ttcaattttc tttttactgt catttctttc
     4441 atcatactcc ttatttgata gcagaaggat caatataaaa gaatcctgta tctaattcac
     4501 ggttttcagg atgcaattta ctgatttcgc tagtcatttt ttccgctttt tcttgatccc
     4561 agctaccttc taaaatttgt ttttttgctt cggcgaatag tcgagtgatg tttacgaatt
     4621 tttcgcctgg agtaaataat ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt
     4681 cattcaattc attcgagaga ttcagatcat tgctggcaaa aatatgtgtg ccatgactat
     4741 cttcataaat agaataatgt gtctcttctt ttttaggttc cgagataaaa agacctcttt
     4801 cacgatcttt ttgttcgtct tgtttcgtat agttatagta attctgtagc agtggacgtt
     4861 tggattgatg gatgcatgta gcaccataca caagggtttc tacaggaata tcgagttttt
     4921 cttccatcgc cttcatctct tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc
     4981 cttttttcgc ccagaaattg atttgacgtg cgctcgttac taatgtttca gcatcataaa
     5041 tatacggtat acgcaattca ggattttttt tcatcgtata aataatacct gtatcaccaa
     5101 ctgtgatcac atctacgttt atttctttta aaaaagccaa atattcaggt acatttttca
     5161 ttttttcagg atgaatgatc ccatttacag caatattcgc ttttttacca tacttgtgtg
     5221 ctaattctac caattgtcgc tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa
     5281 attgctcttc accgaaatat agtgtatctg cacctgcagc aagcaactgt tctgcttgtt
     5341 caatagtttc tactgtggtt atgagttcga tcataatttt ctcctcctac aacttcataa
     5401 tagtagcata gctctaaaaa ctttccaata cttcttctat ttaaagtcgg atttttgaaa
     5461 attatttcac aaaaaatcaa aataaagttc gatatattct gtccaaatat aaaaagagat
     5521 tgtgacaaaa atcttgtcac aacctcttaa tcaagtctta ctattataaa aattcagcta
     5581 cacaggccaa gtaactatag ggaacctact gtttcctttt taggtccgcg aacattcaat
     5641 gtgatcttac cttttgaagc acctactgtt acgtggtcac ctaaacgaat ttctccgcca
     5701 agcaaagcct cgctcaatcg atcttcaatt tctttttgaa gtgcgcgacg gattggacgt
     5761 gcaccatatt caggatcaaa tccagctttc ccgatcacat cgattgcaga tggtgtgatt
     5821 ttcacttgaa tatcttgttc tttcaatcgt ttgataatcg ctttgctcat gatttggacg
     5881 attgtatgga tttcatcttt actcaatgaa tggaagacga cagtctcatc gattcgattc
     5941 aagaattcag gtcggaaagc tttctttaac tcttctaaga tacgtttttg catggctttg
     6001 tgatctttag taacatcttg gacattgaat ccgacatttt tttcttctcg aatttgtgtc
     6061 gctccgatat ttgaagtcat aatcatgatt gtatttctaa agtccacttt tctaccttta
     6121 gaatctgtta aatgaccatc atccaatact tgcagaagta aattgaaaac gtctggatgt
     6181 gctttctcca cttcatctaa gagaatcact gaatatggtt tttgacgaat tttttctgtc
     6241 aactgtccac cttcatcata ccctacgtat cctggaggag acccgattaa tcggcttgta
     6301 ctgtattttt ccataaattc tgacatatca acacggatca gggcatcttc acttccaaac
     6361 atgacttctg aaagtgcttt ggccaattct gtttttccga caccagttgg tccaaggaac
     6421 atgaatgaac caattggacg atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca
     6481 cgtgaaactg ctttgacagc ctcatcttga ccaacgacac gttcatgcag aagtccttcc
     6541 agttctaaca agcgttcgct ttcttttttc tccaactgct gtagagggac accagtccac
     6601 tccgatacaa ctgtagcaac atcttcttct gttacacgat cagaataacc ttgctcctgt
     6661 tttgcttcaa ttgcagaaac tttttgcagc tttttacgta gtgctttttc ttgtctgcgt
     6721 aattgagcag cattttcaaa gtcttgtttt tggatcgctg cttccttttc ttgtactagt
     6781 tcatcgattt ctagtttgat cacagttgat tttgttaagt gatcggtttg atccaaacgg
     6841 actttcgctg ctgattcatc gatcaaatca attgctttat cgggtagttg acgagaagtg
     6901 atataacgaa cagaaagatt gactgctgct cgtacagctt catctgtgat ctccactcca
     6961 tggtgctctt catatcttgt acgtaatcct tgaagaatca cttctgcttc ttcaggagtt
     7021 ggctcatcta cttgaatgcg agcaaaacgt cgctctaatg cagaatcttt ttcgatatat
     7081 ttttgatatt catctagggt cgttgctccg atcgtttgta attctcctcg agctaacgcc
     7141 ggtttcaaga tattcgaagc atcaattgct ccttctgctc cacctgcacc aattaaggta
     7201 tgcaattcat caatgaataa gattacttga ccatcattat agatttcgtc gatgaccttt
     7261 ttcattcgat cttcaaattc accgcgatat ttcgtaccag caaccaaaga tcccatatca
     7321 agcatcatca ggcgcttttc ttgcatgtct tctggcactt cacctaaaat gattttttga
     7381 gctaaccctt cagcaatggc tgttttgcct acaccaggtt cacccaccag tacagggtta
     7441 ttctttgtcc gtcggctaag aatctggata agccgtttca cttctctcga acgaccaaca
     7501 actgggtcca gcttgttttc ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc
     7561 gtaggtgttc cttgcggtgt ttgccgttta tcttgtacat ttacatttct acgacgattc
     7621 attccttttc cagcagaatc accagctagc cccatttttt gtttcagaac ttgacgggct
     7681 tttgaaaggc tgatacctaa gttgatcatg attcgagtag ccatgatact ttcatctttt
     7741 aataagctca tcaaaatatg ttctgttcca actttttgag aaccagtttt cttcacttcg
     7801 ctgctagcta aagcaaaaac ttgtttcatt cttggtgaat atggaagaaa gactccttta
     7861 gggtaagaac ggataccacc ataaccaatc agatgttcaa tttcttcata aatttccgtt
     7921 tcattcaaat ccatttcacg taatactttt ccagcaattc cattttgctc gaccactaag
     7981 gctagtaaaa catgttctga actaactgat tgatgtttca accgttttgc ttcttcttgt
     8041 gcaatttcta atactgcttg ggctctttca gtaaaaagtt cttccatcta atctcacttc
     8101 ccatcttcat aacttaaacg ttccagaaat gctcggagaa tccgagcacg tgtatgatct
     8161 tcatattcat tgaaattcaa tgtgtttttt gcaattgcac tcaacatcaa gtttccttct
     8221 ttttttgtaa ttatttggtc ttcataaagt ttttgaataa tagcaaaagc atttttctca
     8281 ctgattgtat catcaaataa ctgatttatc tgctcaagta attgtttttt atctgaaatc
     8341 cttacttttg caattcgtat ataaccacca ccaccacgtt tgctttctac tagataacca
     8401 cgttggatgg taaaacgggt attaatcaca tagttgattt gtgaaggaac acagttaaag
     8461 aggtctgcca tttcaatcct tcgaatctcg atcatttcac tattttccag aattttcttt
     8521 atatatgatt caattaaatc cgaggtattc tgatgtgcca tcttattcat tccctttgac
     8581 taatattgac cttaattata acgaatttga taatagaaag aaagaagaac gctttttgtt
     8641 aaataaatcc tttagaatca acaaaaaaaa atacggaata tactattccg tatcgacgcg
     8701 cccaagagga gtcgaacccc taaccttttg atccgtagtc aaacactcta tccagttgag
     8761 ctatgggcgc atattcaatt taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata
     8821 aaaaaaatcg ggaagacagg attcgaacct gcgacccctt ggtcccaaac caagtgctct
     8881 accaagctga gctacttccc gtttaaaaaa taaaaaagac
//
LOCUS       DAJSAW010000086         8691 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJSAW010000086 DAJSAW010000000
VERSION     DAJSAW010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.087"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            123..515
                     /locus_tag="ONT64_002521"
     CDS             123..515
                     /locus_tag="ONT64_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303906.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(688..870)
                     /locus_tag="ONT64_002522"
                     /pseudo
     CDS             complement(688..870)
                     /locus_tag="ONT64_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            887..1054
                     /locus_tag="ONT64_002523"
     CDS             887..1054
                     /locus_tag="ONT64_002523"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303907.1"
                     /translation="MENTFELYNKTTVDKLIPILVPTDLNGHDVLLRFNGFLGEDYRY
                     LKISDPQPLTE"
     gene            complement(1097..1672)
                     /locus_tag="ONT64_002524"
     CDS             complement(1097..1672)
                     /locus_tag="ONT64_002524"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR2303908.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1827..2353)
                     /locus_tag="ONT64_002525"
                     /pseudo
     CDS             complement(1827..2353)
                     /locus_tag="ONT64_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2810..4321
                     /gene="gadC"
                     /locus_tag="ONT64_002526"
     CDS             2810..4321
                     /gene="gadC"
                     /locus_tag="ONT64_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HCR2303909.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            4334..5734
                     /locus_tag="ONT64_002527"
     CDS             4334..5734
                     /locus_tag="ONT64_002527"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR2303910.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5899..7339)
                     /locus_tag="ONT64_002528"
                     /pseudo
     CDS             complement(5899..7339)
                     /locus_tag="ONT64_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            7732..8325
                     /locus_tag="ONT64_002529"
                     /pseudo
     CDS             7732..8325
                     /locus_tag="ONT64_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
     gene            complement(8309..>8691)
                     /locus_tag="ONT64_002530"
     CDS             complement(8309..>8691)
                     /locus_tag="ONT64_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2303911.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
BASE COUNT         2760 a         1447 c         1566 g         2918 t
ORIGIN      
        1 attttttctt ttaagtaaaa aatataggtg attttttttg acttcatatt tataataaag
       61 acacgtaaat caattatttt tcattttact ttaactaata atgtttattt ggagggatta
      121 ttatgttaga tgatagattg acgtgttcta attgtggtac attattttac tatgaggaag
      181 ttagaaatat tgtaaaacat agaaaaaaag aaatgcctat acgttgtcca aattgcaatg
      241 aagttattaa gcgaaagttg tctcatggtt attttgtcag ctacgaacta tatgaagaaa
      301 gtaaaataaa tctgaaaaaa aaaccaaaat actataaaaa tttgaaaact ggagctatta
      361 taactgaaaa agaatttaaa aatttagtga ctgaagaaga aagcaggttt tataatttgt
      421 ctggtaatat ttgccctgga tttatggatc agattattga aaatgagtca attgaatcaa
      481 ttgaaaacta tgttcctgtt gacttaccaa aatagaaaaa ataatcctgt ttttacagtg
      541 aattatttta attttgattt tcaataaaaa tatattacaa taataatcgt aatagctaat
      601 gaaacaattt ctcaatcttt ttaccactcc aaacagggag cgtcaataat tttgtgtaaa
      661 taactcgtcc ttctgcaaaa taatgggtta ctcagtaaac attgaagcta atgtatccgt
      721 cacttgttgg aagcctttat ggcttctatt taagaatttt tgattgtatg tatcaaagat
      781 ggatactagg aaacgctcta gtgattcttc attttgaaac tgctcttttc tgcggctgta
      841 tttcttgatt tgtttattga aagattcgat tgtagaacca gaaggaatgg agaacacttt
      901 tgaactttac aacaaaacta ctgttgataa attaattcca attttagttc caactgattt
      961 aaatggtcat gatgttttac ttcgttttaa cggattttta ggtgaggatt atagatacct
     1021 aaaaatttct gatcctcaac ctttaacaga ataataaaaa acgtgtaatc aattatttga
     1081 ttacacgttt tttatttcaa tcttcgttgt agtagtggtt gcgacatacc gaaaggtaat
     1141 cttcattacc tcctactaca atctgttcac ctgaataaac tggaatacca tcaacaatac
     1201 gagcattcat tacagccttt ttattgcaaa aatggcaaat agttttaatt tcctcaattt
     1261 tatcagcaaa tgtcaataat gcttcggatc ccttgaagag attattttga aaatcattct
     1321 tcaatccata gcaaattact ggtacttcct cattcattac gataagtgat agatcaataa
     1381 tattctctct acttaaaaat tgagactcat cgaccaaaat acaatcaact tttccgtact
     1441 gatcaataat ctccactaat tctccgtaca catccatttc agaagtaatt gcaatagctg
     1501 gtttcgaaac acctatacga ctctcaactt ttccaaatcc tgatcgagta tctattgcgc
     1561 ttgtcattat gataactctc ttcccttgtt cttcataatt atgagccact ttcagtagct
     1621 caatactctt tccactattc attgttccat atctgtaaaa taattgtgcc attaaattta
     1681 ttcccttctt ttacaattca attattcctt aattccatct gcttttatgc agctgctcgt
     1741 ttattccttg atatgtctat aacatctact atccctaaaa ccaatttata agccattaga
     1801 gatggtattc ctgaacatct ctcatattgt tttttgagtg gtatatcatc tgaaaaactg
     1861 aaggaatcat gaaaatagcg acatacttat catttagagt tctaacttta aatgcattta
     1921 cttcttcagt cagcacttta gtcatgttgg acatggtttg gggcgtgtag tgatgcccat
     1981 acattttctc aatcaagtct gcgatttccg acatggtaat accttttcgg aagaggtgaa
     2041 tgacggtctc ctctaatgtg tcattcgtcc gtctataagc aggaacagtc tgttgcttga
     2101 actcgccatt gcggtcgcgc ggaatctgga gatgaagttc cccatactcg gtcttgaccg
     2161 tacggtcata ggagccgtta cgagaattac ccgtgttaaa accaatgcga tcgtactttt
     2221 cgtaatcgag gaaagccgtt aactccgttt tgagaagcgt attgacagct ttttccaaat
     2281 ggacacggaa taatccattc aaatcgcctt tgttcgctag agtctttagg atttctgtag
     2341 taaaatcgtt catatggaag tcctcttttc tgtgaatgtg ttgtggtaac tctattctac
     2401 agaagggact tccttttttc tatttacaca aaatatttta cactctcaac aaaagtatct
     2461 tcgattaata cttgctcaag ttatgtgcag ctacgaaaaa gtaggtaccg aggtactttc
     2521 tttttcgcag ctttttttgg acttatgaga agtacattcg ctggaagaat acgatataaa
     2581 acgagctttt tcactaaaga tattaataga gtgaaagaaa caagcttttg ttgtcagttt
     2641 tttcactttt ctaaaaatga aaataaaaaa ttctggaagt ggagaagaat tttgcttgct
     2701 atactttgtc ttgtagaaaa gaggtagaca aaataatgca agctcaaata agcgattttc
     2761 cataaccttg gtaggaatga caaattaaat aggaaaggga agtaataata tgaataaaaa
     2821 aaagttatct ctattcggtt tttttgccct gacagcttcc atggttttga ctgtttatga
     2881 gtatccgacc tttgcaacat cacaattaca tttggttttc tttcttcttt tgggaggtat
     2941 tttatggttt ctaccagttg ctttatgtgc tgcagaaatg gcaacagttg aaggatggaa
     3001 agatggtggg attttcagtt gggttagtca aactttagga gaacgttttg gttttgcagc
     3061 tatttttttc caatggtttc aaattacagt aggttttatt acaatgatct acttcatact
     3121 aggagcgctg tcgtatgtaa ttaattttcc agagcttaat acagatccat ttatcaagtt
     3181 tattggtgta ttagtcattt tctgggtact gactttttca cagttgggcg ggaccaatcg
     3241 taccgctaaa attgctaaag caggatttgt cattggaatt gttattcctt cgatcattct
     3301 ctttggttta gcagcagcct actttatcgg cggtaatccg attcaaatgg ctattaatgc
     3361 gaaagctttt attcctgact tttctcagct ttcaacttta gtagtatttg tatcttttat
     3421 attggcttat atgggcgtag aagcttcagc atcacatatt aatgagctgg aaaatcccca
     3481 aagaaactac ccattggcaa tgtttttact agtgattttg gccatcgcct tagatgcaat
     3541 cggcggtttc tcagtagcag cggttattcc acaacatgac ttgtctctca gtgctggtgt
     3601 aatccaaaca tttgaaacgc ttattttaca ttttaacagt catttacatt ggttggtaaa
     3661 agtgattgct ctcatgattg cctgtggcgt tatgggtgaa gttagttctt gggtagttgg
     3721 tccatcacga gggatgtttg cagctgctca gcgaggtctg ttaccagttt tcttccggaa
     3781 aactaataaa caagacgttc cagttccgtt gattatgata caaggaatta tcgttactat
     3841 ttgggcagca gtcttaactt ttggtggagg aggcaacaat ttatcattct tagtagcaat
     3901 ctccttaact gttgtcattt atctcgtagg atatttacta tttttcctag gttactttgt
     3961 attagttttt aagaaaaaag acttaaaacg aacttacaat gtaccaggaa aaactgttgg
     4021 caaaacgatt atcggtggaa ttggttttat tttatcagtg ttcgcattgc ttatctcttt
     4081 tgtaccacca gcttcaattg cagctagtga gcaccgaaca taccaaatca tactacttat
     4141 tagctttgta gtgactgttc ttttaccttt tatcatttat gaagtacatg ataaaagtgc
     4201 tcacacaact attgaggaac ctactcacgt gaaagctgaa gacgttaatc ccgctgttta
     4261 tccagctgct agaggagagc accacgttac aaaaaatgat gaacatattt taaaacatta
     4321 ggaggaattc attatgttat acggaaaaga taatcaagaa gaaaaaaact atttggaacc
     4381 aatttttggc tctgcaagtg aggatgttga cttgcctaaa tataagttaa acaaagaatc
     4441 cattgaacca cgaattgctt atcaattagt acaagacgag atgttggatg aaggaaatgc
     4501 gcgattaaac ttagctactt tttgtcaaac gtatatggaa cctgaagcag tgaaattgat
     4561 gacccaaacg ttagaaaaaa atgcaattga taaatcagaa tacccacgaa caacggaaat
     4621 tgaaaaccgc tgtgtaaata tgattgctga tttatggcat gctccaaata atgaaaaatt
     4681 catgggaact tcaacgatcg gctcttcaga agcctgcatg ctgggtggta tggccatgaa
     4741 atttgcttgg cgtaaacgtg ctgaaaaatt aggtcttgat attcaagcaa aaaaacctaa
     4801 cctggtgatc tcttctggtt accaagtttg ttgggaaaaa ttctgtgtat attgggatgt
     4861 ggaactgaga gaagtcccaa tggatgaaaa acatatgtca attaatctag atactgtcat
     4921 ggattatgtg gatgagtaca caattggtat tgtaggtatt atgggtatta cttacactgg
     4981 tcgttatgat gatatcaagg gtctgaatga tttagttgaa gctcacaata aacaaactga
     5041 ctataaagta tacattcatg ttgacgctgc atcgggtggc ttttatgcac catttactga
     5101 acctgatcta gtttgggatt ttcaattgaa aaatgttatc tcaattaatt cttcaggtca
     5161 caaatatggt ttggtatatc caggtgtggg ttgggtctta tggcgtgacc aacaatactt
     5221 accagaagaa ttagtattta aagttagtta cttaggtgga gaaatgccaa ctatggctat
     5281 caacttctct catagtgcag cacaactaat tgggcaatac tacaactttg tacgctatgg
     5341 ctttgatggt tatcgtgata ttcaccaaag aactcatgat gttgctgttt atttagccaa
     5401 agagatcgaa aaaactggta tttttgaaat cattaatgat ggatcagaat tgccagttgt
     5461 gtgctataag ctgaaagaag atcccaatcg cgaatggaca ctatatgatt tatctgatcg
     5521 tctgttaatg aagggatggc aagtcccagc ctacccatta cctaaagact tggatcaatt
     5581 aattattcaa cgcttagttg ttcgagcaga ctttggaatg aacatggctg gtgattatgt
     5641 acaagatatg aaccaagcaa ttgaagagtt gaataaagct catattgttt atcataaaaa
     5701 acaggatgtg aaaacgtacg gctttactca ctaaaattct tcagagaaat ataaaagaag
     5761 ttaattaact aataaaagga gttgtgatta ccacacagct ccttaaatct atttgaataa
     5821 atatatgata ttcatttgtt acactctcaa tcttagctgt tgttctattc atcattactc
     5881 ttattgtaag tagttcagtc aaatttgctt gttcaattga tcgataacag tatgaattgt
     5941 ttgaaaagta atcggatata taattgtttt ctcactacct aagccttctt ggtataaaga
     6001 actatcgcaa agtataatcc gctgagtaca aaagaaagtg tcgtctacta tttctctaag
     6061 ttgtttacaa atgatattac taatagaaaa gtgttctccc aaaatgtctt ctaactgttg
     6121 tcgatagtat aaatacctaa agtctgacgg agctactatg aacacttcaa ttggagattt
     6181 ccgtttatgc tcatttaaaa gacttaaact aatggttagt ttacgaatag aattctcatt
     6241 ccaacgcaga aataaattgt tttgttttcg ccattgttca agaatttcat gaacttctaa
     6301 gtacaaagcg cgttgctctt catttaacaa gtaagtctta tcaggttgaa atatctgaat
     6361 atcagcccaa agtgagcgga taaacgggag aagtgcttta tcaaaaagta tctgattttc
     6421 ttcagatata ttcagagata agataagttt ttgactgagc tcaatcacca aagggttatc
     6481 tgctacatgg ttttgataaa caacgttcaa gtctttttgt aacaattcaa agttatcgca
     6541 tagataattt ctagagttga atagaaagta gatataaagg cattcatctt cgtcatgaca
     6601 taaagagaaa gaaagttgct ctaatccttt ctgtattagc tgaaacaagg gagtatgtcg
     6661 ggcaactctt ttttcttcct gactaaaata tattgggtta gtcttagatc tctgcaaacc
     6721 gatagcgata cctaataaaa taaattgttt ttcaactgta gggtaacttc ttgaaaaaaa
     6781 atgtttttct acataatcaa tcaattgttg accgctttga ataattgatt tatttagctg
     6841 gatcaactct gtccgatttg tctcatagat tatcgttagt aacaaacttc gataatcctt
     6901 ttcaggaatt tcaaaatggc catcagcatt taagtactgc atttgtttaa aaaaattaat
     6961 caactcattt cgtaagttgt aaactttgct taacgaaatg aaggtagtat cagctatatt
     7021 actccaattg tgttcccctg ataaataatg tcctagtaca ttgaaaaagt cagagcgctg
     7081 atagatgagt tgagtgagtg ccaacctcga aaacgaagag tcgaatgcga ctgtaaagct
     7141 acttctagat ttctccactg aaataatttg ctgatggagt atgtcggtaa tgcattccaa
     7201 gtcgttagtg acagacagtg agtgggcaaa cgttcaattg atctgctatt tcgcgaatct
     7261 gaatggtccc tttttcgtgt aattgctcac aaatgtaaac ttgacgaaaa atacttttct
     7321 ccagataggt ttccaacata gtactcctcc tagtcatttt atacataact attataactc
     7381 taccaatcgg aatagtcctg taatatattg aatcaaaaat ataaattata aaataattag
     7441 atggggtatt tggtttaagt atcccttata tagtaataaa tttataggtg tgattctcga
     7501 attaataccc gaaaatgtga gagaattttt atggattttt taggatacaa attaattatt
     7561 tttgctttta tcctgattac cagtccagta ctgtcggtat cccattttgc agcaaaatca
     7621 tgttgataaa gtgacaagaa gactgatgag atatattatc caaattgaga agataattaa
     7681 tcggattctt gagtataccc tactttttaa gaaagtaggg tatacaagcg tatgaataaa
     7741 gacataactt tccctgtgat ggacgttcgg cttgttccaa ttaccaaaat acaggcgaac
     7801 gattataatc ccaatcgagt agcgtctccg gaaatgaact tattggagct ttctatcatt
     7861 gaagatggct ttactcaacc attagtttgt tattacaagg aaaaagaaga gaagtatatc
     7921 ttggttgatg tgcttcatag gtatcgtgtt gctaaggaaa gactcaactt aaaagttgta
     7981 ccggtaacag ttattgataa acccatataa gaacgtatgg cttcaacaat tcgacacaat
     8041 cgtgcaagag ggacccatga aataagagga atggaaattg tcatccaaag attagtaaaa
     8101 aatggatggt cagacaagag aatatccaaa gaattaggca tggacattga agaaattttt
     8161 cgctttaagc aagtgtctgg tggaatggca acagtttttt tgacaaattt tataaggtgc
     8221 agaacttctt tccgtatgct attccgattg tccttgacaa tgagcctcct cggatgtgac
     8281 gatcttcggc aggagctaac agttaaagtt agactgcctc gctagcgtta gcacatccga
     8341 gctcattatc aaggattcgc tgcgccaatc tctggttacg gtaaccaacc ggtgtctcgt
     8401 agccaagtgt accgtgcaac cgaaggtggt tccaccaatt tacatagtca aataactcca
     8461 aatccaattg ttgtaaggtt tcaaatgtgt attgatagac aaattctact ttcaacgact
     8521 tataagttga ttcagctacg gcattatcaa aaggacagcc tttatgactc aatgatcgat
     8581 tgatgtcaaa agttgttaat aattcatcaa tagcttggtt atcaaactct tttccacgat
     8641 cagtatgaaa aatctcaacc tctgtcagag gttgtttgat acggctaaat g
//
LOCUS       DAJSAW010000087         8518 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJSAW010000087 DAJSAW010000000
VERSION     DAJSAW010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.088"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..377)
                     /locus_tag="ONT64_002531"
     CDS             complement(<1..377)
                     /locus_tag="ONT64_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR2303912.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSI"
     gene            complement(383..1489)
                     /locus_tag="ONT64_002532"
     CDS             complement(383..1489)
                     /locus_tag="ONT64_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HCR2303913.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            complement(1489..2625)
                     /locus_tag="ONT64_002533"
     CDS             complement(1489..2625)
                     /locus_tag="ONT64_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR2303914.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(2657..4108)
                     /locus_tag="ONT64_002534"
     CDS             complement(2657..4108)
                     /locus_tag="ONT64_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCR2303915.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(4152..4916)
                     /locus_tag="ONT64_002535"
     CDS             complement(4152..4916)
                     /locus_tag="ONT64_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HCR2303916.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(4944..5642)
                     /locus_tag="ONT64_002536"
     CDS             complement(4944..5642)
                     /locus_tag="ONT64_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HCR2303917.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(5654..6433)
                     /locus_tag="ONT64_002537"
     CDS             complement(5654..6433)
                     /locus_tag="ONT64_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HCR2303918.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(6449..7393)
                     /locus_tag="ONT64_002538"
     CDS             complement(6449..7393)
                     /locus_tag="ONT64_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR2303919.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7439..8218)
                     /locus_tag="ONT64_002539"
     CDS             complement(7439..8218)
                     /locus_tag="ONT64_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303920.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2421 a         1771 c         1272 g         3054 t
ORIGIN      
        1 tttattgatt ttacacaagc ttttacaatt tccggcgatt gatccaatcc ctgccaccaa
       61 caaacccaaa tgcagttaga atatggtgat tttgattgat taattatttg attttgacta
      121 taatcatatg ttttcaaaaa atctgaaaaa gtcttttcat aatacttcag cattacctca
      181 tgttttttta ataaatgttc cttctgtttt ttactttcat tatatccatt acgattcatg
      241 acttgaatat caattttagc ttgtaacgtg gcaaatgctt ccttccaaga tgttatcttt
      301 gcaattcgtc ttgttgcatt caattcttcg gatatgcgat gtataattct ttttatactt
      361 gaatgtttct gtgtcatatt ctttaacctc tttgtattaa ttcaaataac tcataatatt
      421 ttgattctaa gattttgtat gctgtttgaa tattataatc tgcctttatt aaaatcgaac
      481 gttctgaatc tcgatgagag atatctacat ttgcaatctc tctcgcccat tcttttgccg
      541 attgatttaa acttatgaaa ttggtcttat cactgaaagc aacttctgta ggaactttct
      601 ccgaaaagaa acacggtaaa tctgcaaatt gagcttcaat acccactaac ggaacccctt
      661 caaataatga tggcaacatt aatacgtcaa atgcttgata aaatttttct atatcatttc
      721 tatttcctaa aaatttaata tcatcatcta tgccactttt ctttgcataa ttttttattt
      781 cttcttccat atcaccagtt ccaataatta ataatacgga attattctta atagcgtgaa
      841 attctttaaa aatttcaatc aaaaaacgat gatttttaga atatgaaatt cttccaatat
      901 gtcccattac aaacgtctga cttgaaattc ctaatttcat tcgtacatca tttctaatac
      961 tttcatcata ataaaatttt ttagcatcca ctgcatttgg aatcaaagtg aatttatcat
     1021 ttcgaaacaa aaaatcacct gctacttttc cacatgaaaa ttcatgtgtc aaaacagacg
     1081 ttaacttaca tcgataatat tgctttaaaa gatatttaaa atctctatct atcgatgtat
     1141 tatggctatg tgctatccgt atcggaatcc cagctttttt tgcagctttt aaaggtaaat
     1201 aactcatcgc atcaatatga gaatgaacaa tctcatattc tggatgatcc ttaaaaaatt
     1261 gttccatcca tttaaaatat ttaggataat tttgtggata aagtctcggt gcataataga
     1321 cctttcctcc aagacttatt atttcctcat cataatcact tttatatgga cgatgtgtca
     1381 aaaaatcaaa ctgaatttta gttcgatcta tattcctata atagttcatt agcatcgtct
     1441 ccaaccctgc acgatgcata tcattcacac actgtaatat tcgaatcatt atatatttcc
     1501 cctatacaaa tttaatgttt cttgtacgat tttatcccaa ttatatttac tacaaataaa
     1561 ctcagtagct tgattcttca aaactttaac catttccgat tgattgcatg cctcttctaa
     1621 tcttatcttt aaatcactta catccgattt cttaaaaatc atagccttgt cttctactac
     1681 ttccgtacac tctgagatat ttgaaactat acaacagttc ccataactca ttgcttctaa
     1741 caaacttaat ggcattcctt ctaaatcact tggaagtgta tagatatacg cattactata
     1801 taattcttct aattcctgtc cttgtacaaa tccagtaaat ataattcgct catcaccttt
     1861 tgctaattcc tttagttcat ttgcaaattc atctgtatca gagcttcctc cggctattat
     1921 aagtttttta tctgtctgaa catctttaaa tgcctcaatt agataccgaa ttcctttttc
     1981 gggaaccaat cgccccaaaa acaaaatata agaatctttt gttaatccat acttttctgt
     2041 aatcaatcct gctgatttta tttcaatttt tttaacgcca tttggtataa accttgtttt
     2101 tcttccatat atatcttcaa aataattttg gacgctttca ctaagtacaa ttatttcatc
     2161 tgcaaatttt acagcacact tttcccctaa catgatgtat gtactagcaa gcttgttcca
     2221 tttttctctt tgatgatcta gaccatgaat tgttgcaaca catcttttcc caaaaagttt
     2281 tggcaaccac aacatagcac atggtccttc tgcatgaaaa tgaacaacat catattttcc
     2341 gaatgctgct ctaattccac caaatactga agaactcatt gctgcaattc cttttatatt
     2401 taatgtaaaa atagtcttta attttacacc tttatattct ttatttttat gctggtcaaa
     2461 ttcctttccg ctaacatgat gaccacttcg attatagcaa gtcacttcat gcccaagtgc
     2521 aaccattcgc gttgccaatt cttctaccac tatctcaatt ccaccttctc ttgatggaat
     2581 tcttttatga cctagcatac agatttttaa tttttttctt gccatcacgt tttactcctt
     2641 ctatagtatc tttatcttac atggaaccgt ttctatgaag cacagttttg actgtcctaa
     2701 acaaaattct aaaatctaat cccatactcc aattcctgat atattctgca tcaagtttta
     2761 caacctcttc gaagtctgtg atatcacttc taccactcac ctgccacatt ccagtaatcc
     2821 caggtttcat cgcaattctc gcacgatgat gaaaatcata cttctcatat tctgacttca
     2881 agcaaggtct aaaccccacc aggctcattt ttcccattag gacattataa aattgtggaa
     2941 actcatccaa tgatgtagat cgaataaatt gtccaattcc tgtcttcttt gttccatcag
     3001 gaagtatttt attgccaatg attcttgggt caaaatccat cttaaacatg agattattac
     3061 tcatcttatt ttctttcatc aactctgctt tgcgttcttc tgcatccata tacatactac
     3121 ggaacttata catcttaaat gtcttaccat tttgaccaat acgttcttgt gcaaagaaaa
     3181 tcggccctgg tgatttgatg taaataactg gtgctacgaa gataaaaata acacctgttg
     3241 caatacatcc aataattcca gcaagaatgt caatcgttct ctttataaat gcttgcctca
     3301 tcgtcataac attaatacta gatgtaataa cagtataatc accaactcgg ccaataaatt
     3361 gttttccacg tgtactatca gacacttttg taagattcat atgtacagca agtcccattt
     3421 ccgaacatct ggcgattaat tctttgggat atggaacact ttcgtcaaca ttgatgaata
     3481 cttcatctac ccaattctgt aaaagatatt ccgctgcatt atcagcattt gcaactactg
     3541 ggacacctcc gatggtagtt ccatacatat ctctgtctac tacaatcaca ccactaagtt
     3601 tatacatgcc ataattcttc tctttaagat tacgaactac attttttgca atcttttgag
     3661 atgttacaat aactaacgat acatcttcac cgtttttcat gcggtgatgc aacaaccctt
     3721 tccaaagtat acgaattata tatgttaata atccatacac cacccccatt ataaataaag
     3781 aaactctgga ataatcattt ccatcctgca ttgtaataag gtataaactg cacacaagta
     3841 acagcataaa agattgcttt atagaagcta cagcctcttt ataatatcct ctttttaaaa
     3901 catttttata tgcctcaaat aaaaatataa tcgtaagatt ctcaagttct ataaaaattg
     3961 ccatgtggcg ataaatagga atcacatacg gattccagtc accctgtcgc attacatatg
     4021 atatataaaa agcaagctgg acacaaaata agtctaataa aataaaatca atatgtttag
     4081 accaaccact tgagtttttt ctatacatct ctcactctct cctcatttcc tttttatcta
     4141 cgaataaaga actaaaataa accaaacttc ttctttttta cctcttcata gattggataa
     4201 ttgatctcat ccccgttgat cagatccttt gcgacttgtt tcatctgtac tactttttcc
     4261 ttaccaaact ccttggcaat ctgctcatag cactctttta agtaaaaggt gcgcttagag
     4321 accccgtgtg catcagaagc agccatctgc acgagattat gtttgaccat tgttttggct
     4381 gttttctgga tatctttgcc aaatacacca acataacttg gcgcagtaag ttgggccaaa
     4441 catcccatat ctaggaacgg aagcaaatga tttgggtctt ctcgaaaatg cgcgttgcgt
     4501 tctggatgga cgatcactgg tatctttcct agttctaaca actcaaaaaa aagccgttct
     4561 gcatataacg gcacttccag tgtcggaaat tcgatcagaa gatatgtatc atctaaatct
     4621 gtaaataaaa tttcatctcg gtggatgtct tcgatgagcg ttccggtaat acgtacttct
     4681 tgtccttcca ataacgtcaa aggcaactgt cttttctcta attcggcttg taacgaagcg
     4741 actagagaaa tgacttgtga tttttcattg ctgtattttc cattattgtg gtgtggtgtg
     4801 cataaaatat gcgtaatgcc ttgctgaatc gctttttcag ccattgcaat acttgcttct
     4861 aagttttctg ccccgtcatc gattcctggt aggatatggc aatgtagatc gatcatttct
     4921 cttgtctcct ctcttcttat tccttaattt ccgtagtaat aaccagcatc tttgctgtgt
     4981 tctgctccgt tgtatacgac acctaagact cgtgcttgta ccatatctaa cagttctttt
     5041 gctttagtca atgattcttt tcgtgacgta ttttcccgta cgaccaataa ggtgccatcc
     5101 gtttttgaag acatgatctg tgcatctgtt acggcaacga ctggcggcat gtcgaagatc
     5161 actacatcaa atagttgtct tgcttctgct aaaatctgat ccattcttgg cgaacttagt
     5221 agttctgatg gatttggtgg tttaggtcca cttggcagaa tcgacaaatt gtcgactggt
     5281 gttcgttgga tctcatctgc tacacttcct gatgaactaa gaacagtact taatccgctt
     5341 gcgttgttca acttgaacgt tttatagatg acgggcttac gtaagtctgc atcgactagc
     5401 agcacccgtt gaccagattt ggcaaaaaca acggcaatgt ttgctgcagt ggtcgatttc
     5461 ccttctccag gtcctgatga agtcacaaca atcgttttga tttgctgacc agctgcggag
     5521 gcaaactgga tattcgtccg aatcgtacga tattgttcag cgattggtga agagggattg
     5581 gtgagtgtga ccaaactgac tgcatgagtt tgggctgctt tttgtttctg tgttcgtgcc
     5641 attttctttc ctcctagact cttgagcggc ttctgcgtga tggcgctgtt ttatcgtttg
     5701 tagcttttgg ttgtgtctga gctggtcctt ttttacggat accagcattc aattctttcg
     5761 ctgacatttc tggaactgtt cctaagattg ggaatcctaa tgtatctgtc acatattttt
     5821 catctttcac tgtccgatcg agtaattcca ataagaatgc cagacctaca cctaccatca
     5881 ttcctaacat taagccagtc attaaattca atttattatt tggcgaaaca ggtgtcgtat
     5941 tggcaactgc acctgaaatg atcgagattt tatcaacatt catcacatct tttgcgttct
     6001 cttggaaaac ttgtgaagtt gtgttagcga tctgttgagc agccactgca ttcgtactta
     6061 ttgcctgaat cgagaacatt tgtgaatttt ctgcctgatt aactgagatg gcatctttga
     6121 tttgttcagc agtcattttc acattgtctt ctgtttctaa acgatctttg acttcattga
     6181 gcactaaatc actgacgatc atatctttat acgtattgat catttgcagg ttcgtattga
     6241 catcattgac atttgcagtg tctgattgtg gcagtgtcac gatcaattgt gcttgtgaac
     6301 tgtattttgg tgtcaagatg aaaaatgtta cgattcctga gatggccaag ccagcaaaca
     6361 ttgagatgat gattattacc aagtgtttgc gtaatgtttc aaaaatttct ttcaagctga
     6421 ttgtctcttc cattttttct ccactttcct atttattctc tagattcatt tgatctttta
     6481 attctttttt cacacgatca agttcttgat cactgacttt ttgataggag atattatctt
     6541 gcatgaagcc ttctccttgc agctgatctt gcttaatcgt accaaatgcc tcccgataat
     6601 ctaaagcaat ttttttcatt tcatcaaagt tcatgtctgt tttcatattc gactctagag
     6661 cattcaaaac ggattgatag ttgctgacac cgtttagact caatactttt ttcgctacgc
     6721 cttctacgat ctttctttgg cgcccttgtc taccgtaatc accatttggg tcttcataac
     6781 gcatacgaga ataagataat gcctgatccc cgttcaatgt aattttaccg attggaaagt
     6841 catagccatc ttgtgagaaa gtgaggtcat tattgacttc gataccacca actgcatcaa
     6901 cgagttcctt tagccctttc atattgatag agacatagtg atcgattggg atgtctacat
     6961 atttttgtac ggtatccatc gccatggcag ctccgccgaa tgcgtaagcg tggttgatct
     7021 tgtcttttgt gtcacgacct acgatgtcga catacgtatc acgaggaatg ctgacgatcg
     7081 ttgtctgttt gtcttcagga ctgactgtgg caaccatcat cgtatctgat cttccttgat
     7141 cgacccgacc taaatctcca gtatcaatcc ccatcaatag aacagagaaa gattcttgtt
     7201 tgtccagatc aactggtttc gttcgttctc cttgggttcg ttccactgat tcgtaagttt
     7261 tctcaataga atgtgagaga tccagatata attttgctcc tactcctaca attgctaata
     7321 ctaatacgat aagaactgct aaaataatca gaaatatttt tttccctctt gattttcttc
     7381 ttcgtcttgc catagtttcc tccgtttgct aaccttgctt gaatcaacgt tttaactgtc
     7441 aatagcttat attgctatag cccatttgct cagcaacagt ttgtgctttt gttgcgtatg
     7501 ctgcgcttat tttttcattc cggatctgct ctatttgact gagcaccgtc aaatattgat
     7561 cgtaagtagc ggtggcagtc acttgaccat cctttaacat cgtatcgaat aactgatcta
     7621 gttgcgtgac ttgcgcgatc tgaatatcag cgaatcctag atattgcata acaaccgttt
     7681 tccaaggact atctttaaaa aagctcatat tttcacgtac acgagcgaca tctgcacttg
     7741 tcaccttttc tttgatgatg acatcgtcta cagcttgctg ccagtttgat acatttttag
     7801 taaataaatc gttgacagct gtctggatct tcagttttga ttctgcacta tctaactgtg
     7861 tcacgattgc tttcttctca tcttggattg caacagcttc tttaggcaat tctgattctt
     7921 tgatgtcgta atcatctgct gaaagtttga cagaggatag cttatttgac aaacttgtga
     7981 ttgtttcttc tgttatttca gggtttagaa aaattttttc ttttgtacta tacagcttgt
     8041 ctacttcttt tgataaagaa gtcaaggtgt ctttttgatt ggtgatggca gattctgcat
     8101 aattgatccg ttgctgatgt tgccgctgat tattgatatg gtatccaaac aaacagatcg
     8161 caatagcacc tgcacctacc gccacagcag tcaatgactt gacgaatggc ttttccatag
     8221 attcactcct aagtattgat gatagataaa ttaagatata attcacttac ataaaaatga
     8281 cagcttttta tttaaaaaaa cataattaaa ctaatatatt cacagaaata tttgtaaaca
     8341 agttttccct atataatcat accacaaact gttttcttga ctagggcaat ttacgattta
     8401 aaagtaaata aaacattaat aagataaaaa ttaatcgatt tcatttttat ttccataatt
     8461 ttcataggtg tttttaaaag ttacacaaaa aatagaataa caaaaaaatt aacattga
//
LOCUS       DAJSAW010000088         8489 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJSAW010000088 DAJSAW010000000
VERSION     DAJSAW010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.089"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..173)
                     /locus_tag="ONT64_002540"
     CDS             complement(<1..173)
                     /locus_tag="ONT64_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2303921.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="ONT64_002541"
     CDS             complement(593..1714)
                     /locus_tag="ONT64_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR2303922.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ONT64_002542"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ONT64_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCR2303923.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ONT64_002543"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ONT64_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HCR2303924.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ONT64_002544"
     CDS             complement(4304..5215)
                     /locus_tag="ONT64_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2303925.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ONT64_002545"
     CDS             complement(5232..6239)
                     /locus_tag="ONT64_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR2303926.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ONT64_002546"
     CDS             complement(6236..8365)
                     /locus_tag="ONT64_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR2303927.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8489)
                     /locus_tag="ONT64_002547"
     CDS             complement(8370..>8489)
                     /locus_tag="ONT64_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2303928.1"
                     /translation="GQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1485 g         2677 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgacc
//
LOCUS       DAJSAW010000089         8328 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJSAW010000089 DAJSAW010000000
VERSION     DAJSAW010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.090"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(61..1020)
                     /locus_tag="ONT64_002548"
     CDS             complement(61..1020)
                     /locus_tag="ONT64_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2303929.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            1116..1661
                     /locus_tag="ONT64_002549"
                     /pseudo
     CDS             1116..1661
                     /locus_tag="ONT64_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1844..4519
                     /locus_tag="ONT64_002550"
     CDS             1844..4519
                     /locus_tag="ONT64_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCR2303930.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            4701..4940
                     /locus_tag="ONT64_002551"
     CDS             4701..4940
                     /locus_tag="ONT64_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303931.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            4956..6329
                     /locus_tag="ONT64_002552"
     CDS             4956..6329
                     /locus_tag="ONT64_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2303932.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            6333..7967
                     /locus_tag="ONT64_002553"
     CDS             6333..7967
                     /locus_tag="ONT64_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2303933.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            8130..>8328
                     /locus_tag="ONT64_002554"
     CDS             8130..>8328
                     /locus_tag="ONT64_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303934.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2874 a         1338 c         1644 g         2472 t
ORIGIN      
        1 tgaaaatcac ctttataaac tccattcttt tccactgttt tattaatatt ttctgatttg
       61 tcaaattaag ctagacaata tgtctttatc tatgcagctc aaaaatactt ccaatggtgt
      121 tcgataattt aatgattttc ttggaatatg gtttcttttc gaggcaacgg attgaataaa
      181 ggtttcatcc gtgtccctaa agtccattgt tttaggtaag ccatctcgtc taagcaagcc
      241 gtttgagtgc tcatttagcc ctctttgcga aggtgttcct ggatctgcga aaaagatggc
      301 aatatcgttc gagttactta tagatcgcca gttggaaaat tcttttccac aatcaaatgt
      361 catggattta aacaagtgtt taggcatgga tttgaaccaa tcatttaacc tgttttcgat
      421 atcttcggct cgtctgcctg tcggtttcaa agtaataata accttggata aacactcaac
      481 caatgtaata acagcgctct tgtgtttaac tcccacaatg gtgtcgactt cgagatgacc
      541 aaattcttta gtcaggtgtg gatagagact gttccgttca tgaatcgttc tgcgaaaggc
      601 ctgttttcca cgcttttctt gatgattatt cggtttacgt ttccccttca taggtaacga
      661 ctgtgtgtca aaaaggcctt ttttaaacat ccgatagagt gtacgaacag aacaatcaat
      721 tggaaactcg ccacggccga taatcacatc gggtgaccat ccttgagcta ccttttcttg
      781 aacatatgtt gattgttcat cagataaaac ggttgaacgt cgtccacaac gagatttatt
      841 ttgtttgtat tgacggtaat aatctagcac tttttttcct tgtttgagaa acgaataaac
      901 attataaatg gtttgtctag agcgatttaa actttgtgcg acaagagcga caggtttctc
      961 gatattgaaa taagactcta tgattactag ttcgtctgtt gtaagatggg tgtaagtcat
     1021 ttgtactcac tccttattaa ttctttggtc ggaactattt tgagtgtagc acaaatgatc
     1081 tttttagttg tctagcttaa ttttacaatc gacgttttca aaatgctgag actattgaac
     1141 gagaacatca ttatttcgat ttattagtga atacagtggt agaattagcg aaatgggcaa
     1201 agtcaaacga aacttatagt caagtactta aaagaattat gagtcaagaa acttcattta
     1261 tctatgagtt aagcaagtca aatttgaaat atacttataa tgaagatgaa cttgaaattt
     1321 tagataatcc agaaaattca caatggatta aagagttgaa gagagaagac tttattgaga
     1381 cactaagaat tctaaaatcg ttatttgatt tgaaattagc caaagctacc atcatgggaa
     1441 ttgaaagaga ttcatattat tgggaggaaa ttggactata tgctccccag attgaaggtg
     1501 aagataaaga gaaaaggata gacaacatta ttaactatat taatagttta agtgaacctt
     1561 taaaatgtag aggacttgat tttataggaa cattaggatg gacagaaaca tactatgaga
     1621 aaatatataa agaaataatt gaacaatatt ttcctaaata atttaataag gattgattta
     1681 tcgtaaaaga aaaaagttgt gatttccaaa atcacaactt ttttcttttg tcaagatttt
     1741 gagtgggata tatttttgta gaactgaaga tcatgaaatt atgagaggtt ggaggtaatg
     1801 tagtcataca aataaaaatt ttttagaaag tgagtggtaa ttaatgaaaa tgaatcacac
     1861 ttcaattcag aagcatttac aactgttaaa tacactgggg atttcttatg ttaatttgag
     1921 aactttttct acattaaaca agaaaaccgt ttctcatatt ttttctactg cagattctca
     1981 agcaattatt aactatttag aaaaacagaa attgtctgaa ggtacagtaa acataactta
     2041 taatcctatt aaacaagagg tgattacaca aaaaaagcat tctgtgagag acaatgatat
     2101 tgaaaaaatt cggtttgtat ttattgatat tgaccctaaa aggaaagagg gaagtgcaac
     2161 agatagcgaa aagaaaaagg cagaaaatgt tatggaacaa gtagaacgtt atttgaaaga
     2221 aaagagaata gaatcatttg taaaagttga tagtgggaat ggctaccata tttttcttcc
     2281 cataaatgaa caaccaaata atcacgagac gattcttact attcagaatt ttcttcaatt
     2341 acttcaccga agatttggag tagaagatga agttgatatt gatacttctg tttataatcc
     2401 ttcaagatta tgcaaattag ttggtacacc agcggtcaaa ggaaagtcca caaaagagcg
     2461 tccgcatcgt ttatctagtt tcctttctat tcccgataac atagatagaa gtaattgttt
     2521 taaaattctt gaacagatag ttgaagaaaa ttccatgatg tcaacctcta gagtaaaaag
     2581 ccatccatct tttagagaag tgttaccttt tattcaagca gactataaac aatggttagc
     2641 tcactatcct gaattaactt ataccataaa agagggcgat cataaaggag ttaccttact
     2701 tatatttgac gaatgtccat taagaaaaca cacgaataat tctaatggtt cttgtttgtc
     2761 tgttacggct gataataaga ttcgatttca ttgtctgcat gcttcggata aaaataaaga
     2821 tattcaagac tttgtaaaaa aatatccgtt gccaaaacct gtaaatcctt ctgtagaatc
     2881 attagaatcc aatcaagaat attattttga ccagtttaaa ctgaatcaag aaggagtatt
     2941 tttggtgaaa gaaggtcaag aagaaaaagt tgcttgtccg atgtttatta agcaaatacg
     3001 caaaaatatc gaacaaaaag tcattacact tgttcttgcc tattatgtcg atcattgttg
     3061 ggatgaaata gaaatagaag gtggatgttt acaaactaat aattttaaac aattagcaaa
     3121 acatggctta atcattaaag ctagaagaga agctgaagtc gttgactttt tacagattca
     3181 acgggagaat gctccaatct tttttgaaca tgaatctctt ggctggaaaa aagaaaatga
     3241 agcaatgatt tttcagttac aagattcttt tggacgtgaa attccttctg tattaactaa
     3301 aaactcatgc taccaattaa caactaatgg tacagaagcg gaatttgacg cattggttca
     3361 taagtatgtt ttaggaacga acttagaact tgcttggtcg attggtattg cttctattgt
     3421 gttaggctat ttaaacgtta cgaaggctaa tacttttttg agtttactaa ttaacctaca
     3481 gggcaagagc acaacgggta aaaccactac gttacacttt attgcctctt tatttggaaa
     3541 tccaactacg gttcttagaa atatgaatgc taccaataaa gccattataa agctagcatc
     3601 aaacaatcag ggaggtgttc cactaatact agatgagtta ggagcagcaa ctcaaaataa
     3661 ctttagctcg ttgatctatc aattagcttc aggagaagaa aggctaagat tagattctaa
     3721 ttgtcagctt atgccacaag agactttttc tgttttgacc cttacctctt ctgaagagcg
     3781 tttggaaaat tatttcaatg agaaattaac tggtttgaaa gttcgatata tcgaattttc
     3841 tgatatcaag tttacaaagg atgcgaatca cgcagagaaa gttaaaagtc tttcttctaa
     3901 tacttttggt tggggtataa agttatttct tttgaaactc tttgaacaag ggattacaaa
     3961 aattgaagca acttttgtcg aagctcaaaa aaaactatgg gctgttttac ctgaacattc
     4021 tttgaagcaa cgcattggta ataatctagc tttgatttat agtggagccc tcctaattca
     4081 agatatactg ggctatcctt tacgattaga gcatatacag acggtgctta ttaattcgta
     4141 cgaacagtta attgtagata ctagtttagg aagtgagcaa acggtatttg accgagtgaa
     4201 aacattgttg gtgatgaata gccataaatt tttaacaaac agtgacaaca aacatacgcc
     4261 cagtacgatt tggggaaaag tatcaattca aaaaaatggg gatattaagg taaatatttt
     4321 tcctatcccg tttgaggact tgttaaggcg agaatttcaa gtaaaggatc taacctatct
     4381 ttttaggcaa ttgcttgatt cgggtaatat gtgtgcagaa aaagggagaa gaacgaaacg
     4441 tattcacatg aatcgtcaag ttcttccaac atacgagatt ctattacctt cttcactcaa
     4501 atcgtatttt cgattgtaat ttagaataaa ccaaagtatg aaagtgaggc accatgttat
     4561 ggaaatattt cttattgatt tagcaaaaca actgattact tatctgtatg atagcgagca
     4621 aacccaaacg gaagtaaatg aagcttgatc tatgcttcat ttacttcatt caatcaaaca
     4681 tgaaagaaag gaaaattcta atgactaaaa ataaaatgac actaaaagca gaggttcttc
     4741 tgtatattca agaacatttt tcaaatcaag ccttttttac aaaaccaatt tacttggatt
     4801 tcgaaataag aggggtatct gctggatcta tcgggggtac tcttcaagcg ttgaagaacg
     4861 aaggatattt agaaaatcac tttgtgcaac gttcgttcaa tagaagagtc gtgaaaaaat
     4921 ggtatttagt tcattcatag agaggggaat caagcatgcg tgtggcttgt tatgatcgaa
     4981 cgtctgttta tgaagaagcg aaaattagta atgacgctca atatgatgca gtgaaagagt
     5041 atgttttaga gaaatatgaa caaattcccc aacgattttc agacaaagga gaatcagggg
     5101 aaaaagcgga gcgtccagct tttcgacagc ttagagcttg ggtttgtgcg tcagaagaag
     5161 agggggtgat ctatgtttgg cgttatgatc gattggcacg tcaagccaaa atagccttag
     5221 aatttattga gttgtgccaa caacatcagg ttcaagtgta ttcgatttct gaacctcttc
     5281 ctgcttcttt ctcacacgcg tcaacgaata aactatttgt gcaattattg ttcacttttg
     5341 cagaacaaca acgaaacgtc attattgaga atgttcgtgc cggactttcc tataaaaagc
     5401 aacaacggca atatttgtct tctgcggtac catatgggta tcgtttagtg aatgggaaaa
     5461 tccaacaaga agtacacgaa gcaaggattg ttcgctatat ttttcaactt tatttgacca
     5521 aaaaatatgg ctacaaaaag ctttgtcaac gattgacgca acaaaagctt ttcttccgcg
     5581 aacgtccttt tcagccctat catatttata gtatcctaaa aaatccattg tactatggcg
     5641 aaatcaaagg aggatctttg gggaagtatc taggaacctt tgaaccgatt ctctcgaaag
     5701 caaccttttt acaagcccaa gagattcgcc aatcaaggtg tacagctaaa aaagatacgt
     5761 atctgtactt attacgtcaa aaaattcggt gtcccttctg tggtcgtcat ctgagttcga
     5821 agtatcaatg gaatacaaag aagacgaaga cgcttcatta ttatcattgt accgaccgaa
     5881 gctgccaagg aatttttctt cgggcagaaa gggttgagtc agaagtattg gaaaagatac
     5941 aggctttttt tcaatacgaa ggcgtctatc aagccttgat tgcccaatta acgcaacaat
     6001 tagccgaaaa gcgaaaagat gaaaaacgca aagtaatcca acaaaagaaa caagaagcac
     6061 aatggattaa ggcatttgaa gagggcaatt gttcactgga agaactcaag caacaactgc
     6121 aagctcttca aaagccgatg tctacttctt cgtttctttc actccaacag tgtgaagcaa
     6181 aagtgaaaga atggcttcaa ctaaaagacc tctctagtcg acaattatta tgtgactatg
     6241 tttgtcggat tgaggtaaca aaggataaaa cagtttgtgg gatttacttc cgacaactga
     6301 cacaaaatat tttagaaagg aacgaatgat ctatggaaat gatacaaacc aaacgagtcg
     6361 cattgtatat tcgcgtgagt acacaagaac aagctttaga aggacatagt attcaagcac
     6421 aagaacaaaa tggacgacag tttgccgaac ggatgggcta tgaagtggta gaagtttatg
     6481 ccgatgaagg aatcagtggg aaatccacaa aaaatcggtt cgcctatcaa cgaatgatga
     6541 acgatgctcg gaaccatcgt tttgatctac tagtgatttg gaaattgacc cgtctcggac
     6601 gcaatatgtt agatgtctta caaacagtcg aggaattatt gcgatatgac gtaggattac
     6661 attcgatctc ggaacaattt gacgtgacca cttcgacagg taaattgatg ttacaactcc
     6721 ttgggagttt cggtgaattt gaacgcaacc aaattgcgga gaatgttcaa atgtcaatgc
     6781 aaagcttagt gaaagataaa aagcgttatg caggaggccg acgtttaggc tatgtcagtg
     6841 gtattgacac gaatggtcaa aaacaattgg tcattgaacc agaagaggcg aaaattgttc
     6901 gtctgatcta tgcaaaatat ttaggcggag cagggtatcg agcgattgcc aattttttga
     6961 atcgtcaagg atatcaaaca gtcaaaaaga atgctttttc taccacggca gtcaaagata
     7021 tcttacgcaa taaaatttat gcagggtatt tagaatatgc gcgctacatc aattgggaaa
     7081 ccaaacgacg gaaggggaag aatccgagac cgattttagt gaaaggaacc catgaaccga
     7141 ttatttcgga agaagattat caaaaagtac aagaacgcct tgctttagaa ggaaaacaac
     7201 ccaaatggaa ccatacaggt gaaaatgtat tgacaggctt gttacgttgt ccagaatgtg
     7261 gcgcaccgat ggcagcgagt aatgtcacaa ataccctaaa ggatggcaca aagaaacgga
     7321 ttcgatatta ttcttgttca gtctttcgga ataaaggagc aagtgtgtgt catgccaatt
     7381 cgattcgtgc cgaacaagcg gaaacatttg tcgccgaccg attaaaggag atcgttcagc
     7441 taccagaagt tcttccccga ttggtggcag cgttaaatga agaaatagtg cgacaaagcc
     7501 aaccattgga acaagaactc gtggtgttac tggaacgaaa ggaagaattg aagactaaaa
     7561 ttgaaaaatg ggaagctgct ttggaagata gtcctgaatt gtttccgatg ttaaaagacc
     7621 gcttagacga attgactgaa aaacgacgac agttgcacat tcgagaaaat gaaatcttag
     7681 ggatctttca acaaggagag ccgattcaag tcaaagatgt ccaacggata ttgactaatt
     7741 tggatcgttt tcttgcccag agtgagaaga agcaaatcaa agccttgtat cgtaccttta
     7801 tagagaaaat aacctttgat cctaaccata aggagatgtt ggaaattacg atgaaattca
     7861 cgcatgccgt ggtacaacaa ttgaatgagc aatatcaaat agccgtatcc aaaacaaagg
     7921 atacggctat ttttgtgtta aaaacaccct ttacgttaac gatctagtct aggttatatc
     7981 aaacaacgtt agaggggcgc taacgcttct ctagcgattt gaaatcgtta gagaagtagt
     8041 tagggaggtc cttatcataa atagggatat ttattaatga atatttgaaa tataattgta
     8101 tataagaatt aaaaagagtg gggatagata tgaaaaagat gggtaagtgt attttatgtg
     8161 aaaaatacac tgaacttact aaagaacatg taccacctaa aaaatcaggg aatactggag
     8221 gtaaaaagac cagaactggt aatttagatg atttccttaa gagcgatttt acaaaaggtg
     8281 attttccgaa ggggattaaa aggaaacctc aaggaaatgt ttattata
//
LOCUS       DAJSAW010000090         7904 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJSAW010000090 DAJSAW010000000
VERSION     DAJSAW010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.091"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..460
                     /locus_tag="ONT64_002555"
     CDS             <1..460
                     /locus_tag="ONT64_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303935.1"
                     /translation="LHHLFLSKTIPRYSKNKKAEKFCLLLNKFTLIAPILAFIIFSVL
                     LSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLLSLII
                     FITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(586..1263)
                     /gene="nth"
                     /locus_tag="ONT64_002556"
     CDS             complement(586..1263)
                     /gene="nth"
                     /locus_tag="ONT64_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR2303936.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1270..1968)
                     /locus_tag="ONT64_002557"
     CDS             complement(1270..1968)
                     /locus_tag="ONT64_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCR2303937.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2236..3420
                     /locus_tag="ONT64_002558"
     CDS             2236..3420
                     /locus_tag="ONT64_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HCR2303938.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3426..3869)
                     /locus_tag="ONT64_002559"
     CDS             complement(3426..3869)
                     /locus_tag="ONT64_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2303939.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4095..4382)
                     /locus_tag="ONT64_002560"
     CDS             complement(4095..4382)
                     /locus_tag="ONT64_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303940.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4553..5905)
                     /locus_tag="ONT64_002561"
     CDS             complement(4553..5905)
                     /locus_tag="ONT64_002561"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HCR2303941.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6178..6624)
                     /locus_tag="ONT64_002562"
     CDS             complement(6178..6624)
                     /locus_tag="ONT64_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR2303942.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6860..7786)
                     /gene="cysK"
                     /locus_tag="ONT64_002563"
     CDS             complement(6860..7786)
                     /gene="cysK"
                     /locus_tag="ONT64_002563"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR2303943.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2146 a         1603 c         1279 g         2876 t
ORIGIN      
        1 tctgcaccat ctttttttat cgaaaacaat acctcgttat tccaagaata aaaaagctga
       61 aaaattttgt ttattattaa ataaatttac tttaattgct cctatccttg catttattat
      121 tttttctgta cttttaagca ctactcttaa aggaaaattt atggaaagaa gctctcatgc
      181 aatgattctc acttttttat ggttgttgtt caccagaatc tatatttttc tgatgagcct
      241 gaaaccgccc aaatccatta gtttatgttt agttataaat ggtatttttt tattatcact
      301 tatcattttt attacacctt tagatagata cgtcacttat ttatataatc ctttggaata
      361 ctggacttat tttataggta tattggaagg cattattttc tacatcgggt attttcccaa
      421 taaaaataac aacttttatt tttatcgcaa taaattgtaa taagttacca tcctagctac
      481 tgagtatgta catttacttt ttcatgcaaa aaagataaac gaaacagctt ttctccataa
      541 taacaagata attcatacat ttatttgcaa ttaaagaaac cacgtctaca ggcgtggttt
      601 cttttttagc gccttttctt ttagacgcat ccgatttttc ccatcttgac atattgatag
      661 caaaggacat acctcgcact tgggatttct tgccgtgcaa tgatatctac caaaaaaaat
      721 cagtgtatga tgtgtcttta cccataattc ttgtggtact ttccgcatca gtgtttcttc
      781 tacttccatg accgtagcat ctagtttgca gatcctcagg cgttttgaga cacgttccac
      841 gtgggtatct actgcgattg ctggtatccc gaatgcatcc cccaatacga catttgctgt
      901 ctttctgcct acaccaggaa gagacatcaa ttcttctctt gacgttggca cttgcccatc
      961 gaaacgttca atcagctgct gtgcacacgc tttgatgttt tttgctttat ttcgatacaa
     1021 accaatcgtt tttattttaa gaatgatttc atcgattgaa gcttctgcta acgcatcggg
     1081 tgtcggaaaa gaggcaaaca aatctggcgt tgctttattc actgatacat ctgtcgcctg
     1141 ggcacttaag atcactgcaa tcaataactc aaatggatta ttgtgcttga gttctccatg
     1201 tgcttcggga aacattccat acatcgtttc tagagcttcc attgtttttt gtttgcttaa
     1261 catattccgc tactcctcca accaattttt cagtgagact ttcggcaatt cttcttgttg
     1321 agctgactgt tctacttctc tttgaagaag ctgctgtttt cttcgttttt ggtcttcttc
     1381 cacttgttgt tttgtacgta gattttttct ttcccacgat agcaaaatgc ggtcaacgta
     1441 attaaaacta taagcctgat ttaatacggc ttcccgcagt gctagcttca aaatttccgg
     1501 ctgataatgg tcttcttcta gccattgtcc gatccgctgg aactcgatcg aagaaagcgg
     1561 gcgtccaaat tcttgttcaa ataattgata cactgactgg atttcttttt cttgtgtttt
     1621 ttcttcgtat tttttttctt gtgtttttaa atactcacct aatagatcat agatggggta
     1681 aagattatat cgatctgctt ttttaccgtt gacaatcgtc gtttctatct tgataaatcc
     1741 atttgttacg agacgatcca gtatttgaaa aatctgatct tgtttgattc ccatatccat
     1801 agcaatcatc tgcaaatcag ggaaagtatc tccttcaagc tgtgccatat gcaactgtag
     1861 aacaaataaa aattcttcag attgtagacc gatcctatga taatttttaa gtaaaagatt
     1921 agatatgact gtttgtccag cgcttaagta attttctaaa ttcaacatga tttctcctcc
     1981 gctcaatcac agtatacgta aaaaaaacat gggaagcaag aacaggtttc ttttctattt
     2041 acttcactga tcggtcagat ttacaatgtc gtctacttga tttttgtttg ttcatgtttt
     2101 tgatgcgtat cttcactttt cggaataaat aaattaagta gatttcgctg ttccttagaa
     2161 tgttgacaag gaaacaaaat tactatacac tatttttgtt tccaagttaa cattattgca
     2221 taaaggagtt tttacatgta cagtttcaca aataaacatt ctaaagtttt tggatacgtc
     2281 tctgtcttat tcactggatt aggatttact atcgtcagtc ctgtacttcc gtttttaacg
     2341 ctgccttatt ctcactccat acatcagcaa gctttctata tcactttgct catgtctgtt
     2401 tatgctctag ctgccttctt atctgccccc attctcggta gcttaagtga tcattttggg
     2461 cgtcatccga tccttattat cagtttatta ggatcaagta tcggatattt gctttttggt
     2521 ttaggaaatt cgatatggat gttgttcctt ggtcgtatca ttgaaggatt gacggcagga
     2581 gaaatctcca ccttatatgc ttactttgcc gacatcacgg aacctaatga aagaaccaaa
     2641 gtttttggat ggatgggtgc tttagtcggt ataggaacaa cgctgggtcc tattataggg
     2701 ggtctattag ctgagttggg gaattctgtt cctatattta ttggagcact ctttactttc
     2761 ttaaatgccg tttatggcta cacatttatg ctagaaagcc ttccaataaa aaaacgttca
     2821 gtagatttaa gtttctccca tgtaagacct tttcaccaat tacaacaatt attcaagatt
     2881 tcatccgtta ttcctttatt gacagcaggt tttgctgttt ggttggcagc tggctctctt
     2941 caatctatct tttctcaatt ttcgattgac gcctttcagt ggaaggctgg gttggttggt
     3001 ttaagcttct ctttgattgg aatcttggat actgtctcgc aattgttcat tatgcctcga
     3061 ttgctcaaaa aattttcaga acagcagatc acaagaatag ggatgttcag tgaaatcctc
     3121 gcctatctat tcatcacatt atctggaatt cttctactgc cgattttgtt tttatttggg
     3181 attatctgtt atggtttcgg tgattctatt tttactcctg tattcaatgg ccaactatcc
     3241 aattctgtct cagataacca gcaaggtctt gtaatgggcg gtacccaaag tatacaatct
     3301 ctatcgcgcg tcatcggccc gctaatcgca ggacagcttt atgctgcagc accatttctt
     3361 ccgacggtat tcggattttt gcttatgatt tttgcctatt atcactacaa taaaaaatag
     3421 tagcttcatt tgctacttcg tataaattct ctgtatcgac cgataaaacg aaatactgtg
     3481 tcaaattctt cttttgacat attttcaaat acagtagcat ctcgctgtac ccatttttga
     3541 tggagttgct cgtgtctatt ccttattttt tgtccgattg gcgtcaattg aaaatagatt
     3601 tcttttttat tctcttcgct ttgataagtg tttatgatcc ctctagctat cagttttttt
     3661 gttagcttac tgatggctcc tctagtcatg taactagcat tcgctaattt tgtcacgtta
     3721 ggatcaggga ttttcccgat atgttcaatc acttctagtt catttagagt aaatcctgca
     3781 aattcttttt ccatttctgg tttattacga tgaataatct cattagctaa atccagatat
     3841 tcttctatta gcagttgtct tttatccatt tctacacctt ctttttcttt cattatattg
     3901 tttttttgtt tcctattcaa caaagataaa aatattttgt aggaaaaccc aatctgaaac
     3961 caagaaaaaa aggggttgac aaagcttgtc ataacccctt tttctgatta aaagatagta
     4021 aagagacagt catcggtaat tgtgatgatt ttttgtcatc acttaaggat gagcggcctt
     4081 ttcaaaaact gtctttaaag atcatcatca gtttctagca catatggctg accttcatat
     4141 gtgtactctg tattcaatgt gccgtcattt tcatctgagt agatgcgtct actgaaagga
     4201 aaacgatgag aaacattatc tttggcgtca tttgctggca tcgtgtaata ttcctcttgc
     4261 gtttcattca actgagagag tacttgatct gccaactcat caatattgat cgttaatttc
     4321 ttcgaagcac ttttataatc ttctaaaaat gcatcttgtt ggacaaaata ttctttattc
     4381 atcttctttc tcacctttct tttcaaaatc aaataaagct gcttgttctt ttatgtaaca
     4441 gctttatctt tgctgcttat tgtgacaaaa aattcgaaga aagacaacta attgtacttt
     4501 tcacaaaaaa agacagaggg tcgtgacaaa attgtgtcac aacccttttt ctttattgtg
     4561 cagtttcgat ttcgcctagc gctgcttgtg caagtaggtt caagtaattc catgggcggt
     4621 caaaatgtgg ttggaagaag aaatctgaaa gtgcgagatc ttctacagtc attttatttt
     4681 gaacagctaa agacatggta tttgctgatt gtgtaatgtc gtatttggac atgaactgag
     4741 cacctacgat acgattcgtt cctttttcat agactagctc catcattact ttttcagtag
     4801 ttggcatgaa ctctggacga taattatctt caaatacagt cgcttggact tctagatcgt
     4861 tcaaagttgc gctttctttg gtcacacctg tcgaaccgat cgtccagcca aataggtaaa
     4921 gtcctgaagt tccttgagta ccacgatacg ccattttatg atctgtcaag ttatttccca
     4981 caagcaaacc ttgacgaacg gcatttgttg ccaaaggaat atagttttgc gtgccacttg
     5041 gattgtagtt gactactgca gagtctccag ctgcaaaaat gtctggatta cttgtctgca
     5101 tgtattcatt cactttgatt gccccgtttg gcagtgtatc tacttttcct tcgatcagtt
     5161 ttgtatttgg acggaagccc acacataaaa tgaccatatc tgtatcaaat gtatctgttg
     5221 gtgtagctac ttgtttcact tttccttctt catctgtgat aaattcagta acattttcgc
     5281 ctagcgcaag agtaacaccg cggtctgtca attctttttc taagatatct gtgaatggtt
     5341 tatctagata tttattcaag atgcgatcta atccatcaat caatgtcact tgttttccag
     5401 attcgacaaa agcttctact aattcaatac cgatatatcc accaccaaca attactacac
     5461 gttttgcttc tttcgcttgt gcaataattt catttgcttg attgtagttt ttgcatagga
     5521 gtacattttt cgcatcgatt ccactaatcg gtgggataat aggccatgaa ccagttgtca
     5581 tcactaattt atcataagaa accgtttctg tttctcctgt tttcagatct tttgccgtta
     5641 ctgttttatt ttctgtatcg acgtctgtta catcgtgttc cattttaact gttgctccta
     5701 atgaagataa ttcttcagga tttgagtaga acaagcctgc tgggtccttt actactccgc
     5761 cgacataaag tgcgataccg caagataaga aagaaatatt gtcatttctt tcatatactg
     5821 tcacttctgc ttctggatga ttttttaaaa tactttttac agctgcagta cctgcatgag
     5881 tacagccaat tactactact ttcatttatg gtccctccga ttaacatttt gtccttcgac
     5941 aaaactacta taacagaaat aaaacggata caacagttta atgcttgtga attcacaaag
     6001 gtttgtttgt agtttcattc cttttcaagc ataagaaccc aatatctaca agattcttat
     6061 tttaataata ttcactttta tatctaagat tgtgaataaa agacaaaaaa ataaaaaagc
     6121 tagcgttttt tttacacagc tagctttttt taggtataac acttgtagaa actgttttta
     6181 catttgcgcc ttttttgctg actctttaca atccggacag agtccataca attccaaccg
     6241 atgttcatta atctcaaaat cagtcagtct gcttgcagcc atttctatat cttcaagccc
     6301 tgggtaatga aaatcaacaa tcttaccgca gttttcacaa attacatgat aatgacgctt
     6361 tgaactgaaa tcaaatcggc tagaagcatc gccgtacttc atttcttgaa caaatccaat
     6421 cgaagtaaat aagcgaaggt tattgtaaac agtcgcaaca ctcatatttg gaaaccgatg
     6481 ttcaagtgct ttataaatct catcagccgt tggatgggaa tgatgttcaa tcaaatattc
     6541 taaaaccgcg taccgttgag gtgtgattcg aatgttctct ttttttaact tttcgataac
     6601 ctcttcgact aacttatttt ccatgtaaac accccacgtt gttattctcg tcttatcata
     6661 ctatttttac attttaaaat ccacttaaca tgaaaagaaa attcaatttt attgtatttt
     6721 aatgaaaaaa gacgtaaaac agtcgcgttc actattctac gtctctattc aaataatttt
     6781 tctcatcata aatttttgtg tgttcttttc atctttaaaa gttttttaca taaacctatc
     6841 tggagaaaaa ccaagtgcct tatatctctt ggtaaagggc tgtagataaa taacgttcac
     6901 cattgtcggg aaccacagct aataccttct tgccttttcc taattctttt gcgactttta
     6961 gcgcggcagc aattgcagcg ccagatgaga tgcctactaa aatcccttct tttcgcccaa
     7021 cttctctagc tgtttccatc gcttcgtctc cactaataga aaggacttta tcatacacag
     7081 aagtatccag tgttttagga acaaaacctg taccgattcc ttggattttg tgaggtcccg
     7141 cttctttccc ttctaaaata gcagattctg ctggttctac cccgattaat tcgattttcg
     7201 gatacacacg ttttaattct cggccagcgc cagtgatcgt tcctcctgta ccgatacctg
     7261 caacaaacgc gtccaagcca tttactccaa atgcttgatg tatctcagga ccagtcgtct
     7321 tctcatgcac aagaggattt gcttcatttt caaattgtaa tggtaaaaag tagccgtttt
     7381 cttttgccaa acgttctgct tctctgattg atccagaaat accatccgca ccaggagtca
     7441 aaatcaactc tgcgccgtac cctttcatca ataaacgtct ttcgatactc atcgtctcag
     7501 gcatcacgat gattacttta tatccttttg caactcctac catcgacaat ccgattcctg
     7561 tatttccaga agtaggttca atgatagtat cacctggttt cagtagacca tcgtgttctg
     7621 ctttttctat catacttaaa gcaatacgat cttttacact tcctcctgga ttaaaaaact
     7681 caagctttac aaaaacatct gctgaatctt ctggtacgat tttatttaat ttgacgattg
     7741 gcgttttgcc aatcaattcg gtgactgagt tatagatagc tgtcatcttc aattcctcct
     7801 tgtctttgct cttcttactt atcataatac aatctgttca tttatggcga gtgattcgtc
     7861 tttgactagc aaaaaaaaga caaagatata ccatatatgg taat
//
LOCUS       DAJSAW010000091         7810 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJSAW010000091 DAJSAW010000000
VERSION     DAJSAW010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.092"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            118..2445
                     /locus_tag="ONT64_002564"
     CDS             118..2445
                     /locus_tag="ONT64_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2303944.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2571..2900
                     /locus_tag="ONT64_002565"
     CDS             2571..2900
                     /locus_tag="ONT64_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCR2303945.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2940..3944
                     /locus_tag="ONT64_002566"
     CDS             2940..3944
                     /locus_tag="ONT64_002566"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR2303946.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3941..4387
                     /locus_tag="ONT64_002567"
     CDS             3941..4387
                     /locus_tag="ONT64_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCR2303947.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4537..5352
                     /locus_tag="ONT64_002568"
     CDS             4537..5352
                     /locus_tag="ONT64_002568"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR2303948.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5558..6730
                     /locus_tag="ONT64_002569"
     CDS             5558..6730
                     /locus_tag="ONT64_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR2303949.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6892..7386)
                     /locus_tag="ONT64_002570"
     CDS             complement(6892..7386)
                     /locus_tag="ONT64_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303950.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
BASE COUNT         2645 a         1465 c         1543 g         2157 t
ORIGIN      
        1 ccttagaata aaaagagaaa aaaagcccca aactttctat acatcaataa aataataaaa
       61 attaaattta gaataacgtg taaaggatta tttgatgatt ttttattgag aggagggatg
      121 aaaatgatta agaaaactca aaataaaatc caacgtatat gtcaaaaaca ttttgtttct
      181 agactcaaag ctgtatctaa aagaaaatac ttacatccca ttatgatttc gatcatcttg
      241 atttttactt tcttagggag cttatcaagt atttatgtaa acgtttatac agaaacgtca
      301 aattttgcta accaaacgac agaggagaca aatagtccac cctattctga aatacagtcc
      361 atagaaagca ccaatcagtc tagcaacaac gagacaacct caaccacatc ctcgactcaa
      421 ctgattgatc cgacagaaaa agaaatcaca gcagcaaaag cagcagggga gaaagcttat
      481 tcagaaacag gtagaagcca gacatttagt actgttgcgg ccactgcgaa tttcaacgga
      541 cataccatac cagatggata tacagtgata tatgccaaag atggagtcat tgttgcggtt
      601 aacggatcga tgagtgcgga taaaactgcg ttacaaggcg taaccaaagc aatcgctttt
      661 attgataacc gtccagatat ccataatctg tttattggct ccacagtaaa tggtactact
      721 atcacattcc caaaaagtat ctatatgggg gtggctaccg ctggaaatac gatcgattca
      781 gttacaccca atgcggataa aagttattct tttacaccct caacatgggg agggcttcct
      841 gccggaggag gtatgagtac tttttcaatc ggtatacagt tagcaaatga tattccattc
      901 acggggtact cgatcaaaaa tgggcagatc gttgatggta agggaaatgt ttcaaatttt
      961 ccaacctatc aagcagcaac ggttcaggtc aatgcgaatc ggacaggaac tttttcttat
     1021 acagtcaatg gtggagcagc aaaaatcgga acgtacggga ctacattatc tggaatccat
     1081 gtgggtgaca aagtggttat cactccaaca gcagtaacgg gctattcttt tgcacaaaca
     1141 aacggttctt tcacagccct agaaagtaac gtcgatacgg tgacctatac aggcaaacaa
     1201 acaccaatca ctattaagta tcaagactat tttggacaga tgattgctcc ttctaaaata
     1261 atgaatctta cttacggaag tgctgcacaa gacctgacga ctaatacgcc tactatcaac
     1321 ggatatacct ttacagctgt ttcagcgata gaaacaaaga accaatcagc aatgagtgtt
     1381 ttagctagtt tagatacaaa tggaaatgta atcgtaaagg acgcaaatgg aaaacagatt
     1441 tctgaagtca tcctatatta taagacaaat gcaacagtat caatcaatgc aaatggcagt
     1501 aagtactatg atggtctttc tgttgtaccg atcgttaaat atgcctttaa tgataaaaca
     1561 gaatcaacat cccttttaaa caatgatctc agtattgatc aaatcacatg gaatccaact
     1621 gatttcaaag cgatgaatga aaagggagtt gaagtaactg cgcctactga aattggaacc
     1681 tatacgtctt ggcaattgac aaaaagcgga ctagctaaac tagctgccag aacaaactat
     1741 ttatttacta tcgtgcagac atctgatgtc tatacgatta agcaaatcag cggtgcggta
     1801 gagctcagtg gcagtaagac atatgatgga aaagcaggtg tcccgtcaat ccatgtcaag
     1861 ctagcagagg gtgtcactag ttcgcttact ccagtacaag tggcacttag cagtatggat
     1921 tacacagcag atgcacaatc agcaaaaaat atggttaatg ctggcagcta tacaattaaa
     1981 ctaacgaata gcggaataga caaagtcaaa gcagcaaatc ccaagttgtc atttactgat
     2041 atagccaaca catcaggaac ttataccatc tataaagcta acgcagtgat tactgtcgat
     2101 gacgctgcct ttaattatga tgcccaaagt cactcgattt cggttggaaa tctccacgtt
     2161 accggtgttg tcttaggcga atcattggat tataccctaa cagttaacag ccggacagat
     2221 attggatcac aaatagtaac gattagttta ggcgaaggaa gtgtaaacaa taattatacg
     2281 attatcgcaa cagataccgc taagttgacg atcaatccga ctctcttgct tccttcgaca
     2341 ggtgggatag gtctactgcc ctttgtcttt ttaggactga tctttatttt ttctggcttc
     2401 ttttatttta tccatcgtaa gaaaaaagca ggtgaacagc gatgaaaaaa acaaaaaatg
     2461 tacgtgtgga tatatttgct gttctctttt ttatcctact ggtctttttt ataggttctc
     2521 cgactgtgtc agctagagaa tcacctatta catttacttt gcataatcga atgttcgaaa
     2581 gcgataagat gccaagtatc agtcaaaacg acggaataaa tatcccggat aactttcaag
     2641 gagtaaaagg gttgaacggc gttactttcg ctatatatga tgtctcggat gaattttaca
     2701 agctgcggtc agaaggaagt agtgtggaag acgcacaaag aaaactagca caaaaaagtg
     2761 atagtgaaaa aatactggca gaaaatgtga caaaaacagt agacgaggaa gagggcattg
     2821 cttcttttag tgcatcggac aaagatgagc aaggtcgtga cgctgtttac cgatttacgg
     2881 aaataaagac ttcagattaa gtaaaagaaa aatcagcacc atttgtcgtt gtgcttcctg
     2941 tgacagattc atctaatcat aaattgacga ccatccactt gtatccaaag agcgaacaaa
     3001 aaatacctgc tgcactcaca ttaacaaaaa cagtagagaa taagcagaca gattttgcag
     3061 atggggataa agtcccttat ctcatcaccg taacaattcc tgagaacatc aacgagatag
     3121 gaacctatac gatcaaagat acagctgatc ctcagttatg gctagaacca gaaacgatcg
     3181 attttttgat tggtggagac aagattcata catttcacac acaaaaaata aaacatggtt
     3241 ttgtcttatc agattctgga aaagatctta gttcgtttgc tggaaaaaaa ttaacgatcc
     3301 gttaccaaat gacattgaaa gaaaacagtg aaaaaacgac atttgataac gatgtacgtt
     3361 tgacgactga caatcaaaca gtagaaacac atcttgccat ccaaacagga ggcaaacaat
     3421 ttgtcaaagt ggatcggcaa acgaaacaaa aactagcaga tgctcaattt ctagtcaaaa
     3481 aaaatgacca atacttagcg caagaaaacg ggataaatca ttgggtagcc aaagatgatt
     3541 caagagcaac gatttttact tccagtaaag atggaacatt ttctgtccat ggattgtctt
     3601 ttggaagtta cgaactagtg gagattaaac cgcctaaagg atatatattg agtcaatcaa
     3661 ccattctctt tgaagttgaa aaaggcagtt atgatccgct tcatcctata ccattggaaa
     3721 tcatcaacga accaaaagca aatccgacga ataatggaaa aacgccagtt cagatgcaga
     3781 caaatggtcc atcaaatgaa aaaaatggag gaagctttcc aaaaacaagc gaagagatgc
     3841 ttggaggttt ctcaatttta gggttattgc tagtcctttc aacagggaca gcttggtttt
     3901 ataaaaaaca acaaaaagga aacaatagga gggaaataaa atgaaaaaca tcaaaacaaa
     3961 aatatggacg tttttaggaa ctgctatcat gctgcttcct ttcgttctcg gcctaggaac
     4021 tgcagaggtt tctgcagccg tatcacctac accagaaaat gttacggtta atttgcataa
     4081 attgaaattc acttcagcac cagaaaatca aataaacaac ggaacggaat tgacatttcc
     4141 gaatagcgaa ccgttgaacg gcgttgaatt caacgtatat gacatcactg ctacgtacta
     4201 tccaagtaaa gacacagcag ttccagcaga cgctacaccc ttcgcaagtg taacgacttc
     4261 tggtgaagga ttagctaacc tgactcttcc aggtaaatct gatgggaaag atgctgtata
     4321 cgtctttgta gaaacaccaa agcccggtgt cgaaacgtcg ccaaatatcg tgttaagctt
     4381 accctagacc ctgacaaacc tttagacacg attcacctat atgctaaaaa tgtcatcaaa
     4441 gaaagcaata tcaaaatcca gaaagctggg aacttttcag gggtagataa gttagcaggg
     4501 gcgcaattcc agatcaaaaa ttcagagaat caattcgtga ccggttttga tgaaaacact
     4561 ggtgtcgctc tctatgatcc tgctgagtca gcgttgacat tcacaactga tactgaagga
     4621 tccatcagtg tcaatggatt aatagacggc aattatactt tagttgaaac aaaagcacca
     4681 gatggttatc aattaaccaa tccacaaaaa gaaactactt ttatagtaaa aaacggaaaa
     4741 ttgcaacaaa caaccggtat catccatggc gcagatgaag ccaatcttgg gaatccaaac
     4801 ggtgtagtca atcgggaaaa tacaactacg gataacacga ttactgtaga aaatgtccaa
     4861 aactacggtg atttccactt catcaaacaa gatgtgaaca cgcaagaaaa attagccgat
     4921 gcagaatttt ctattctaag agcaaaagat ccaactgtta aattaatgaa aaaaatagca
     4981 aaccttgcct caggagaata cccatatgct tggtcagatg aagtcacagg ttctgactgg
     5041 gaagaagtga aattgatttc tgattcagaa gggaaatttt caatcgaagg gctgaaacaa
     5101 ggggcatact gccttcaaga aacaaaagca ccagaaggct acgcattacc taagaatgaa
     5161 gctgcgtaca ctgaatttac agtagatgat gatccaacaa cttctgatga aacaacgatc
     5221 gataaccaac ctaaaggaat cttgccttca actggtggta tggggatcat cgtattcatc
     5281 ttggtaggta ctgctttagt tggaggagca gtgatctatt tcaaaaaacg ccatcaagaa
     5341 acagaagctt aaactattgg aaacaaaaat catacgataa ataaacgata gtgattcaaa
     5401 tggggagaaa ggttgtgaca agtggtcggt ctcttggtat tcgctcaaca aacggcaaaa
     5461 taggaggaac tattttgccg tttgcttcgc taatactcag gtcaaggcgc tgatttcaca
     5521 acctcttcat atacatatta gaaaataggt ggacatgatg aataaaagca gaataagaat
     5581 actggacatc ttcatggcaa tcattttagt cgttgggatc gggatctttt cttatccttt
     5641 tgtagaagat tcactgaatg attttcttgc ccagcaaatg atcatccatt atcaaaagca
     5701 agcatcgaat aagaattcag aggaaataaa aaaacagcag gaaaaaatga caaagaaaaa
     5761 ccaacagttg gcagagcaaa acgtatcacc agggataggc agtttcaacc aagcagttga
     5821 tgcaaaagca ttaaaagatt tacctagtaa tgcctttttt atggaacata tgcttggagt
     5881 catcgaaatc ccaaaaatca atgtcagctt acctatattc gatcagacaa cggaaatatt
     5941 tttgcaaaaa ggaacgtcat tattggaagg aagttcttat ccgactggcg ggaaaaacac
     6001 ccatgccgtt ctttctgggc atcgtggact cccagaagca aaattattta cagatttgcc
     6061 taagttgaaa aaaggagacc agtttttcat ccagatcaat ggaaagacat tggcgtatca
     6121 agtagaaaaa atccaagtgg tcttacctga cgaagtagat tctttgggta tccaaaaagg
     6181 cagagatctc gtgactttgc ttacctgcac tccttatatg gtcaatacac atcgactgct
     6241 tgtgacgggg catcgtattc cttatcaagc aaaagaggca aaaaaagcga tccaaggaat
     6301 tgatcaatgg aaaaaatgga aattttttat ttggtttatc ggtatactac ttggatcaat
     6361 cggcttagtt tggctactca tcgcttattt agattctttg gccatcgcta agcgaaacta
     6421 tcctttatct ttttatgtaa agaacacaaa cggacgacca atcgaaggaa tggtcttttc
     6481 tgtaaaaacg ctcaacggca aacactatat tactagagaa aaagttccat ttgtgaaagc
     6541 ttctgatgaa tacggactag tgagattttc tgatttaaaa ggcaggaact atcgcttgca
     6601 acatgaggag ctcctgctta aaatccatgt aaaacataaa cattcgaaac agttttcaat
     6661 gaaattgaaa aaaggaagat ataaactgcg aaaagaaaaa gaggtgtact atttgattga
     6721 gaaagaatga atgaagatgt ccaaaaaagc agagggaaca gactaaatga aaaaagagag
     6781 aagctgacga aatggacgtg taaactgcta tttttggttt actatctgga acgccagctt
     6841 ctattttttt atgtttcaga agaattttat tattatagta taacttttga attagtttcc
     6901 ataacatttg gagcaagggg ttaaaccacg agcttgagct tcgcttaaag tggcaggggt
     6961 atacgtacca tttccacatt tatgtgtatg gtattttgaa ccggtcggtg tgacatagac
     7021 agtttgttca ttattttgct caggagctgc ggcttgtgcc tgctgagaag cctgttcagc
     7081 tgctgcttgc tgtcgcgcct gttctgcttg ttgctgtgcg attgcttctt gcctcgcctg
     7141 ttctgcttgt tgctgtgcga ttgcttcttg ttgcgcttgt tctgcttgtt gtcgtgcaat
     7201 ttcttcttgc tttttccgtt cttcttctgc ttcttttgct ttgattgtgg aatcaattgc
     7261 agcaagacgg cttgtcaact cttgtttgtt ttcaggtagc gcttggatag ctgctgaagc
     7321 agttgcatag ttttcttttg taggatttgc ttcagcttgt gacaaagcag attccgcttc
     7381 tgtcactttt ttttctagtg cagctttttc cgctttttgt ttctcttctt cttttttctt
     7441 ttcaatttgt tctttcgatg aatctgccgt actttcggag atcgtggtac tgctgctgga
     7501 agcctgagct gaacttgtgc tttgagtagt agacgtagta gcaccacacc cactcaacaa
     7561 acttcctgcg accaaaaaca acgcaactaa ttttttcact aaatttcctc cttaagtaaa
     7621 aaacaaccgt tttcaataaa tgtccttttc aattgtttac ttacttgtta taataattgt
     7681 gttatgaata gtgattgagg aaacttaatc taatcccgtg ttgatacacg ggattttttg
     7741 ctatagttct gttattaagt atgcaataat aaaatatgta cgttatttta ttattagatt
     7801 attgacattt
//
LOCUS       DAJSAW010000092         7600 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJSAW010000092 DAJSAW010000000
VERSION     DAJSAW010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.093"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(943..1356)
                     /locus_tag="ONT64_002571"
     CDS             complement(943..1356)
                     /locus_tag="ONT64_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCR2303951.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="ONT64_002572"
     CDS             complement(1812..2297)
                     /locus_tag="ONT64_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303952.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="ONT64_002573"
     CDS             complement(2436..2651)
                     /locus_tag="ONT64_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HCR2303953.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="ONT64_002574"
     CDS             2875..3675
                     /locus_tag="ONT64_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2303954.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="ONT64_002575"
     CDS             3694..5562
                     /locus_tag="ONT64_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR2303955.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="ONT64_002576"
     CDS             5555..6046
                     /locus_tag="ONT64_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR2303956.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="ONT64_002577"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="ONT64_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR2303957.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="ONT64_002578"
     CDS             6606..>7600
                     /locus_tag="ONT64_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR2303958.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1442 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DAJSAW010000093         6643 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJSAW010000093 DAJSAW010000000
VERSION     DAJSAW010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.094"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(1..859)
                     /locus_tag="ONT64_002579"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ONT64_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ONT64_002580"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ONT64_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR2303959.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKIFRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ONT64_002581"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ONT64_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2603..3421)
                     /locus_tag="ONT64_002582"
     CDS             complement(2603..3421)
                     /locus_tag="ONT64_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2303960.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ONT64_002583"
     CDS             3582..4271
                     /locus_tag="ONT64_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR2303961.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ONT64_002584"
     CDS             4285..5787
                     /locus_tag="ONT64_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2303962.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ONT64_002585"
     CDS             5800..6276
                     /locus_tag="ONT64_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2303963.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ONT64_002586"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ONT64_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaacaccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatatttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DAJSAW010000094         6375 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJSAW010000094 DAJSAW010000000
VERSION     DAJSAW010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.095"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            37..1059
                     /locus_tag="ONT64_002587"
     CDS             37..1059
                     /locus_tag="ONT64_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR2303964.1"
                     /translation="MIAELKPKHERQNFLVRDDRLDHAVAFLWKDPQTNETVGASYQG
                     TFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLN
                     DTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGA
                     VDPFTNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTNQ
                     LVSAHKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERVDGTYDFDRFYQEKGD
                     INAQILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQG
                     RQQERE"
     gene            1070..3274
                     /locus_tag="ONT64_002588"
     CDS             1070..3274
                     /locus_tag="ONT64_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HCR2303965.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            3295..3444
                     /locus_tag="ONT64_002589"
     CDS             3295..3444
                     /locus_tag="ONT64_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303966.1"
                     /translation="MRNTTHKEQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     LELDR"
     gene            3520..4113
                     /locus_tag="ONT64_002590"
     CDS             3520..4113
                     /locus_tag="ONT64_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCR2303967.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            4129..5028
                     /locus_tag="ONT64_002591"
     CDS             4129..5028
                     /locus_tag="ONT64_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCR2303968.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            5031..5516
                     /gene="ssb"
                     /locus_tag="ONT64_002592"
     CDS             5031..5516
                     /gene="ssb"
                     /locus_tag="ONT64_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR2303969.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            5882..6142
                     /locus_tag="ONT64_002593"
     CDS             5882..6142
                     /locus_tag="ONT64_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303970.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT         2324 a         1097 c         1317 g         1637 t
ORIGIN      
        1 ccagagtggc tgattgatta tgcagaaaaa gaagggttga ttgccgaact aaagccaaaa
       61 catgaacggc aaaatttctt agttcgagac gatcgtttag atcatgcggt cgccttttta
      121 tggaaagatc cacagacaaa tgagacagta ggggcgagtt atcaaggaac gttcattgat
      181 tatgaacgtt ttggtgagag aggcacatat aagcatatag ataaaaattc gacagcgaat
      241 catggcttta acctaaagat cggtgaccct aagcagttaa aattttttga gagtagtatt
      301 gatttactta gctatgctgc actaaatcgt gatcagttaa acgatacgtg gttagtttcg
      361 atggaagggt taaaacacca tgtgatcagt cattattttg gtgaggcagt atctgaattg
      421 agaaagaaac aagcctttcc tcaatccatc gagatttgtg tggataatga ccgtgcagga
      481 catattttct acgaaaaaga acaattaatg ggcgcagttg atccatttac gaatcagaaa
      541 gtacgttgtg aacgaggaat cgctaatgat tggcaagtgc caaaagaata caaagtcatt
      601 tatgaagaag tcgcaaaaga agaaaagatc acaccagaag ctattatggc catacacaag
      661 acagaaaaca atctgcaatt aacgaaccaa ttggtttccg ctcacaaagt aaatgcgtct
      721 tttggtcagc agctttctgt taacgattca atagaagcta ttaatttgaa agatatttgt
      781 agagaagtcg caaaagaatt aaaagcttgt gaacgcgtgg atggtacgta cgattttgat
      841 cgtttttatc aagaaaaagg ggatattaat gctcagattc ttttctctta taaagcagaa
      901 caatactaca aaggctataa aaatcatgaa catgaatttg tacctgaagt aaaaaaggat
      961 tggaatgatc aattgaagca tgaaatctac caacaagaga tcagaaagca aaaacgtgcc
     1021 atgttgtttc aacaaggtag acagcaagaa agggagtgaa atcgtcatga tgaaaaccgt
     1081 tattttagca gaaaaaccgt cacaagcgaa ggcttatgcc gatagctttt ctaaagctac
     1141 ccgaaaagat gggtattttg agatccaaga tcgattgttt tcaggagaaa ctgtgatcac
     1201 gtatggtttt ggtcatttag tcgagttaga ttcgcccgat atgtacgatg aaaattggaa
     1261 acaatggtca ttagagcatt tgcctatttt ccctacacat tatcactatc atgttcctaa
     1321 agataagaaa aagcaattca aggtggtgaa acagcaactt caatcggccg acaccattat
     1381 tattgcgaca gatagcgatc gtgaagggga actgatagct tggacgatca ttcaacaagc
     1441 aggcgccgat catggaaaga tatttaaaag actttggatc aactccttag aaaaagaagc
     1501 catttatcag ggattccagc agttacgaga tgcagaagag acctacccaa aatttgaaga
     1561 agcacaagca cgccagatcg ccgattggtt gatcggcatg aacggtagcc cgctgtatag
     1621 tttactcttg cagcaaaaag gcataccagg aagtttttcc ttaggcagag tccaaacccc
     1681 aacgctttac atgatttatc aattacagga aaagattaga aacttccaaa aagaaccata
     1741 ttttgaagga aaagcccaag taatcgctca aaatggcgca tttgatgcga agcttgatcc
     1801 gaatgaaacc caagcgacgc aagaagcatt tgaagattat ttaaaagaaa agggggttca
     1861 attgggaaaa cagccaggta cgatccacca agttgaaacc gagaaaaaaa gtgcggcaag
     1921 tccacgtctt ttttctctat ccagccttca atctaaaatg aatcagctca tgaaagctag
     1981 tgccaaagat actttggaag ccatgcaagg gttgtatgaa gggaaatatc taagctaccc
     2041 acgaacagat accccttata ttacagaagg ggagtatgcg tatctgttgg atcacttaga
     2101 cgaatacaaa cactttttaa aggccgaagc cattcctact cctattcata caccgaatag
     2161 tcgatatgtg aataataaga aagtccagga acattacgcg atcattccta caaagacggt
     2221 catgacggca gcggcttttg aacaattaag tccactgcag caggctattt atgaacaagt
     2281 gttaaaaaca acggtggcca tgtttgcgga aaagtatacc tatgaagaaa caacgatctt
     2341 gacgcaagtt cagcagcttc agctaaagac agtcggaaaa gtaccagtag atctaggttg
     2401 gaaaaagctg tttggcaaag agagcgacag aaaagaaaag gaagaagaac cgctacttcc
     2461 taaagtgaca aaaggagaag cggtgacggt agatctacaa gtccttgaaa aagaaacgaa
     2521 gccaccacaa ccttataccg aaggtacgtt gatcacagcg atgaaaacag ctggaaaaac
     2581 cgtggatagc gaagaagcac aatccatatt gaaagaagta gaaggaatcg gtacagaagc
     2641 aacacgagca aatattattg aaactttgaa acaaaaggaa tatatcaagg tagaaaaaaa
     2701 taagcttgtg gtgacaaata aagggatttt actttgccaa gccgtggaaa aagagccgct
     2761 gttaacaagt gccgaaatga ccgccaaatg ggaaagctat cttacaaaga ttggtgagca
     2821 aaaagggaca caagctactt ttctagcaaa tatccaaaaa tttgtttccc acttgttgga
     2881 agtcgtccca ggacaaatcc aagccactga ttttggagca acgttacaag aggtgaaagt
     2941 agccagtgaa aagcaagaag cggcgcgtca tttaggatct tgtccaaagt gccaggaaaa
     3001 agaagtcata ctttatcaga aggtggcgac ttgcacgtct gaagcctgtg attttaaatt
     3061 gtgggtaacg attgccaaaa agaaactcac agcgacacaa ctgaaagaga tcattcagaa
     3121 cggccgaaca agtcaaccag tcagaggact aaaaggtcag aaaggttcgt ttgaagcaac
     3181 gatcgtttta aaagaagact ttacaacagg atttgagttt tctgaaaaga agaagaccac
     3241 ctacaaaaga aaagtgagac gtaaaacgag ttagaattga cggagggaaa aacgatgaga
     3301 aatacaacgc ataaagaaca agaaatcgaa aatcaaaaag ccaaaatacg ttcagagcag
     3361 gagaaaatgc gtgccagcaa acgaagacta cagcaattga agaatcaaca acggattcaa
     3421 aaagaacttg aattagatcg ataaatcctc ctgttgttaa tattaaacta acatgttata
     3481 gttatgttag tttaatatta acattcgaga gggaaagcga tgaaagataa agtcaagtgc
     3541 atttttgaga aatacaatgg taatctatcc ttgaaggatg ctagaaatga agggttatca
     3601 cctatgacgt tagatcgttt agccgaaaag gacaaaatag aaaaaatagc atcaggtttt
     3661 tataccttaa aagatagttt tgtggatgaa ttatatctcg ctcaaagtat ctatactaga
     3721 ggaatcttct ctcacgagac agcgcttgat ctttttcaag taggaacata tatccctaaa
     3781 aaaattaata tgatgtttcc taaaggatac aatgtttcga aagagaattt agatcagtat
     3841 gccattcaac cgcattacac gaataaagaa aactttgaac tgggaatcac agaggcagaa
     3901 agcttttacg gcaataaaat tttcgtctat gatttagagc gaacgttatg tgatatgtgg
     3961 aatcccagat ataaagcaag cgtagaagta aaacaagaag cattaaaaga atatatgaca
     4021 agtaccagca gaaatacaaa taagctaaga agatacatga atattttaaa atcaccaaaa
     4081 gaaatgactt tgtatatgtt gccactttat tgagaggagt aaaggtgaat gacaccgaca
     4141 caatttaaag cgaaaacaag aaatgttgca gctgagaaac atttaaaccc acaaattgtt
     4201 caaagatatt atatgatgga aaaattatta gaatgtattg ttgaatcgcc ttatcatgat
     4261 gattttgtat taaaaggggg atttctgatc ggatcaaaat acgggttaga aaaccgttca
     4321 accgttgata ttgatacgac ttatcgaaat tcgaaactaa caaaagaaaa attagaaact
     4381 gttttttctg aattaacagc tgctcctaca aaagatggca ttcggtttga attaaagggc
     4441 ttaacagaga cgagggaagc cgattattac ccaggttttc aagcaaagtt tattgctttt
     4501 cttgaaaatg cacgtgtacc ttttaaatta gatattactt caggtgattc tattattcca
     4561 gcagcattga tatatgaaca taaattgatg tttgaagata aaacaattca tattccagct
     4621 tatcctacgg aacaaatttt agctgaaaaa ttacatgcca cgtttagttt cggaatagat
     4681 aattcacgta tgaaagattt ctatgatctg tatatgatcc ctaaactaga acgtatcgat
     4741 gagataaatt tgtataagag tgttcaagga acatttaagg aaagaaataa tcaatcagtg
     4801 tttaagaatt tgtataatca ggaaatgcct caattgatga agaatgaggg attgagggag
     4861 aaatggaaaa gatatcagca agaaaactat tttgccaaag atatttcttt cgaatctacc
     4921 ttaacttctg ttgattcgct gatgcaatca attgtcgagc aagaggaagt agaacgaaaa
     4981 caaaatttta agagatcaca acaaatgaat caagaaatgg agagataaag atgatcaata
     5041 atgtaacttt agtaggtcga ttaaccaaag atcccgattt acgttacacc gcaagtggca
     5101 ctgcagtgac cacttttacc ttagccgtta accgtaattt tactaatcaa agtggcaatc
     5161 gagaagctga ctttatcaat tgtgtgattt ggcgcaaacc agctgaaacg atagcgacat
     5221 tagctaaaaa aggcagcttg atcggcgtcg tcggacggat tcaaacgcgg acttatgaca
     5281 accagcaagg acagcgtgtc tatgtgactg aagttgtggc cgataatttc caactattag
     5341 aaagtaaagc agcgacagaa agccgtatgc acgctgatca atcgtccagc agtccaagcc
     5401 ctaccacctt tgaacaacgg gatacagcga cgcctaacaa caatggattg aacgcttcac
     5461 agaatccatt tggtggacaa tcaatcgata ttagcgatga tgatctaccg ttctaagtag
     5521 cattttctat tggttgcatt tgctacggtt tatgttataa tttagacaac aaaaagaatt
     5581 ttcaatctat aaccaaaaac cactagctgg cgggctagtg gctttctttt acgtttctat
     5641 tataagaatt aacgggtagg cttactgtct ttcttggcaa atttacaatc aagccatttg
     5701 cagatatagt gcgcggctac ttctgccatg attgcaaaaa caaaatcaaa aagaattttc
     5761 aaacctataa cctcctcccc ttgccagatt aggccggggt agcagacagc aagactatta
     5821 tagcataatt gaaatatcca aatgattgga tttttgctat aattttagcg aggtgattgt
     5881 gatgaacaaa tatgttggtt tgcttgataa aatccgagta ataaaaacgc aaccgttatt
     5941 ggttcgcttt actttacaaa cgattcatga atcgattaat tgtgtagttg cagatattga
     6001 aattatcgat aaattgctaa ttatggatga tggaaaatac aatattgccg tcacaggtca
     6061 cttcaataaa cgaaatcagc tagttattgc tagcatgtat gttagaaatc ccgatcattt
     6121 tacgcgatcg atgggcatat aaataaaaga agagttgtag tcctaaatga ggattgcagc
     6181 tctttttgtt ttacatagaa acaggaaaag acctttttag gaagacacaa gtgccttctc
     6241 aattgaaaaa cctttctctc actccaaccg ccaccgccgt tcgagaagaa aggaagtgaa
     6301 cgctgcacgt tcactaagat atggtgtctt gcaaagcaag acaaaataga caaaacttcc
     6361 tctcaccaaa aggtt
//
LOCUS       DAJSAW010000095         6287 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJSAW010000095 DAJSAW010000000
VERSION     DAJSAW010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.096"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..177
                     /locus_tag="ONT64_002594"
     CDS             <1..177
                     /locus_tag="ONT64_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303971.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="ONT64_002595"
     CDS             190..690
                     /locus_tag="ONT64_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR2303972.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="ONT64_002596"
     CDS             787..2634
                     /locus_tag="ONT64_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HCR2303973.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="ONT64_002597"
     CDS             2637..3533
                     /locus_tag="ONT64_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2303974.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="ONT64_002598"
     CDS             3583..3972
                     /locus_tag="ONT64_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2303975.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="ONT64_002599"
     CDS             3959..>6287
                     /locus_tag="ONT64_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2303976.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DAJSAW010000096         6115 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJSAW010000096 DAJSAW010000000
VERSION     DAJSAW010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.097"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1127
                     /locus_tag="ONT64_002600"
     CDS             <1..1127
                     /locus_tag="ONT64_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HCR2303977.1"
                     /translation="YLLNITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKA
                     MGIAGIISTIVTIIANIFFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFE
                     AKDPQLKREMLNYSIPLIATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIIN
                     TLQGIFIQAWQISAIKEYGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYA
                     KDFYEAWKYVPFLLIANVLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLV
                     YKIGVQGATIATVICSYVIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKN
                     YVIEIIIMIILIIINISYIKQLCYRGRNIISMIKSREHKR"
     gene            1163..1879
                     /locus_tag="ONT64_002601"
     CDS             1163..1879
                     /locus_tag="ONT64_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCR2303978.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            1898..3481
                     /locus_tag="ONT64_002602"
     CDS             1898..3481
                     /locus_tag="ONT64_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HCR2303979.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            3616..4194
                     /locus_tag="ONT64_002603"
     CDS             3616..4194
                     /locus_tag="ONT64_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303980.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            4140..4658
                     /locus_tag="ONT64_002604"
     CDS             4140..4658
                     /locus_tag="ONT64_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303981.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            4666..5706
                     /locus_tag="ONT64_002605"
     CDS             4666..5706
                     /locus_tag="ONT64_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR2303982.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            5955..>6115
                     /locus_tag="ONT64_002606"
     CDS             5955..>6115
                     /locus_tag="ONT64_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2303983.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         2216 a          696 c         1183 g         2020 t
ORIGIN      
        1 tgtatttgtt aaatattaca aaattatggc aagatgttta tggattagaa aaatatattt
       61 ttttgtatta tatatcctat gtatggaatc aatttctaat acaatttgct aaagggttag
      121 aaaaagttaa agctatgggc atagcaggta ttatcagcac aattgtaaca ataattgcca
      181 atattttctt cttgcttatt ctgaaatggg gattaattgg ttttttcatt gcaaatattt
      241 tatcacagat tatttcagct acttatctgt cagtattagt gagaatatgg gaatatatcg
      301 attttgaggc taaagatcct cagctaaaga gagaaatgct aaattactcg attccattaa
      361 ttgcaactgt tgttggatgg tggattaata gtacagctga taggtatgtg gtttccttta
      421 tagtaggtgt ctcggcgaat ggcttattgt ctgtggcata taaaatacct caaataatta
      481 atactttgca gggaatattt atacaggcct ggcaaatttc tgcaattaaa gaatatggag
      541 ataatgagac tgcaaaattt tatggaaata catttgttat gattaatttg ttaatgtgca
      601 taacctgttc aagtcttatc atattaacta gaccgttttc acatattctt tatgcgaaag
      661 acttttatga agcgtggaaa tatgttccat ttttattaat agctaatgtt ttaaattgtg
      721 catcaggtct gttaggacct atcttatcag caaaaaaaga ttctaaatcc atgatgtggt
      781 ctgcaatcat aggggcatta gttaatgtga ttatgagtgt agtccttgtt tataagatag
      841 gggttcaagg tgccacaata gccactgtta tatgttcata tgtaatatat gttgttcgta
      901 aacgagctgt gggaaaagat atttttataa agcattatca tattgttgtg gaatcttggg
      961 gattattgtt tgttcaggca atcatagaaa tatattttaa aaattatgta atagaaatta
     1021 taattatgat tattttaatt attataaata tcagttatat taagcagttg tgttatagag
     1081 gaagaaacat tatttcaatg attaagagtc gagagcacaa acggtagacg atgttatttt
     1141 tataaaaaga tataatttat taatggtcga taattatgaa aggaaaaata ttcttaaaaa
     1201 aaagagtata ggatgtcgta tgaaaatagt cgctatagta ccaatgaagt taaataacaa
     1261 aagattgcca caaaaaaaca cgaaagcttt taccaatgga aaaccattgt gttattatat
     1321 actttcaaca cttttaaaag taaaacagat tgataaagtg tatgtctatt gtagtaaccc
     1381 agatattaaa gaatttatac cagaaggtgt aacattttta aagagatcta gttccttaga
     1441 tcaggataca acgagtatta cacaggtatt agccggtttc acaaaagaag tagaggctga
     1501 tgtatatgta atgacacaca caacagcacc atttattagc gaaaaatcta ttgaaaaagg
     1561 actagatgcc gtgctgacag gagaatatga ttctgcattt gctgcaaaaa aattgcaaga
     1621 tttcttatgg aaagacggtg aaccatttaa ttatgaattg gacaatattc ctagaacaca
     1681 ggatttgccg cctttatacg aagaaactag tggattttat atttataatc gcaatgtgat
     1741 gaccgagttg catagaagaa ttgggaaaaa accttgcata gttgaagtag gtgagattga
     1801 gtcagttgat attgatgaag ccgaggattt tatgattgct gatgcgattt ataatcatct
     1861 tattttacaa ggtagttaag acagggggaa ggttaggatg aacaatgtac agattttaga
     1921 ttgcacactt agagatgggg gctattgtaa caattgggaa tttggatttg ataatacaaa
     1981 aaaaattatt tctggactta tagaatcagg aattgagatt atagaatgtg ggtttctcac
     2041 aaataaaaca gtatacaata aaaatgttac aaaatatacg accttagaag aattagcaca
     2101 gataattcct gaaaaaagag aaggaaaatt atttgtagca atgatgaact atggcgaata
     2161 taatatagag gatcttccag attatgatgg ttcatctatt gatggaatta ggattgcgtt
     2221 tcataaaaaa aattgcaatg aagcgttgaa attatgtcgt gagataaaga aaaaaggcta
     2281 cttagttttt gttcaagcta tggtatctct caattataca gatgaagagt ttttagcagt
     2341 aataaaaaat gtgaatcagt ttaaaccata cgctttttat attgttgata gttttggaat
     2401 gatgaaagga aaagatttaa ctcgtctttt ttacatgatt gagcataatc tagaaaaaga
     2461 tatacagata ggttttcact cacataataa catgcagctt gcatattcta atgcacaaaa
     2521 acttataacc gtacaaacaa atcgaagcct gattattgat tcttcggtgt acggaatggg
     2581 aagaggagca ggtaatctta acacagaact atttgtagaa tatttgaatg ataatgcagg
     2641 aaaaaaatat caattaaaac cattattata tataattgat gaaatactaa atagtttcta
     2701 tcagaagagt tattggggat attcattacc aaactatatt tcagcagttc ataatgctca
     2761 tcctaattat gcaagctatc tgtcatataa gaatacttta acaattgaag caatgaatga
     2821 aatattcgaa atgatggatg aagaaaaaaa ggtatcttat gataaacaat atatagaaga
     2881 tttatactac aaatatatgg aaactgggaa aatacaggag gaatatatgg aagaactgag
     2941 aaaatttctc tcagacaaaa aaattctact gattgcacca ggaaaaagtt ctgtagatga
     3001 ggtagaaaga ataaaagagt ttgttaccaa agaagaagtc gtaataatta gtgtaaattt
     3061 tgaatattct catatagata tagattacgt atttttaagt aatttaagac gttttcatga
     3121 attaccgaaa gaaagcagaa ctaaatgtat tgttacatca aatatacaag catcggatgt
     3181 atatttacgg acgagttata aaaagttgtt atctgaaagg gaagttgtaa gagacaatgc
     3241 aggattaatg gcaattagat ttttagccga tatgaatgtg gatgaaatat tcttagctgg
     3301 atttgatgga tattcacata atgaagatga gaattatggt gagcaatcca tggaaattat
     3361 aacaaaattt gcattattag atgcaatgaa cgtgcaaatg agagatgaat tgtcagaatt
     3421 agctaaaaaa gtaaaggtga catttttaac ggatccacgt tgggttcgaa ttgataagta
     3481 gtagtaataa tgtttttaaa attttgataa agagatagtg agagaataaa aatgggtaaa
     3541 gtcttaaata aaaaagaaga aaaatattta agggacgatg gggcgagatg aaactctaat
     3601 tttgaacttt taagaatcat ttctatattg tgtattgttg cttaccatgc taatttgcgt
     3661 ttaaattata atgcagacgg aattatagta catggttcat taactgctaa ccagattttc
     3721 ttgaattata ttggtatttg gggtcagcta ggagttaatt gttttatatt tatttcggca
     3781 tattttttaa gtgagaaaag aaacattcac tttgaaaatg ttttgggaat tgtattagaa
     3841 tgtatttttt atagtgtatt agtttatgga attctttgtg catgtggaat agaaacattt
     3901 tcattgaaca gctttgtaaa agaaatatta tcatttgctc taggtagata ttggtttatt
     3961 acatcctatt tagcattgta tattctaagt ccgtatttaa atatcatgct taacaatctg
     4021 gaaaaacttc agttgcaaaa aataagtatg ttattgttta taggtgtatg ctgttatcgt
     4081 atgttattta aaacatcttt gtttggggat tgtgctgttt taattgcact atatgtcata
     4141 tgttatacac tgaaaaaaac gataataata tttttgagag acacagaatc ataggaacaa
     4201 ttgtatgtat aacagttata ccacttctca tatccatagg aatacttttc aaatttatac
     4261 caaatgcaat ggttaaccag atggttcata ttggttctcc attgatggtt atagcaagta
     4321 tatttttatt ttatgtattt aaaaattttt cattttacag taagtatata aataaacttg
     4381 cgagaaccag tttaggggta tatattttac atgaaaatca ttatttcatt gatggtatga
     4441 agtctagagg cttaatatgg gatgggatat ttggattagg caaaaggttt ttagataggg
     4501 atttttggat ttggtttata attgcagttg tagttgtgtg cataatttgc atggctatcg
     4561 atgttgtaag agaaaaattt gaaagacaga tttttaaaat taagagtgta aagaacctgt
     4621 gtaaacttat gaatcatgtt ataaatatat acccataatt aataaatgag gagaaaagaa
     4681 gtgaaagtta tatcattcgg agaattatta ttgagacttg cagcacccgg ttattcaaag
     4741 ttatttcaaa gagatagttt ggagactaca ttctgcggag gggaagctaa tgtagcggta
     4801 tccttagcaa tatttggata tcaatcagaa tttataacaa agcttccgga ttcagatgtt
     4861 ggatatgcgg cagctaatgc tttgcgttat tttggagtaa atgttgataa tattatattt
     4921 ggagagggac gcatgggact gtattattta gaaaaaggag cagctcagag accatctaaa
     4981 gtaatttatg acagaaaatt ttctgcaatt tcgttagctg atagtgatga atttaagtgg
     5041 aaggagatcc taaacgatgc aaattggttt cattggacag ggataaatcc agcattgtct
     5101 gagaatctct ttgagacgtc cttagatgca tgtcaggctg ccaaaaaaag aggtataaca
     5161 atttcttgtg atttaaatta tcgatccaag ctttggacat cagaggaagc aaaagaaaag
     5221 atggaacaga tactaccatt ggttgatatt tgtatttgta atgaagaaga tgcagaaaaa
     5281 gtgctaggaa ttaaagctga gcatacaaat attaatagtg gagaattaaa taaagatggc
     5341 tatgtgcagg tggctaggga gattcacaag aagtttggat gtaggtatgt agctacaact
     5401 ctgcgtagaa gttattcggc aagtagaaat ggttggagtg caatgttata cgaagcagaa
     5461 aaagataaag cgtatttttc cacagaatac aacatacaaa ttgttgatcg cgttggtggt
     5521 ggagacagct tttctgctgg tataatatat gcgttatgtg aaaatatgtc cggacaaaag
     5581 gctattgaat ttgcgactgc atctagttgt ttaaaacata ctatagaagg tgattttaat
     5641 cgaactacaa ttgaggatgt atataattta atgaatagcg gtggaaatgg tcgcgtgttg
     5701 cgttagtcaa catataaaga taaatcacag taatttttat ggtgattatc tgtcttagta
     5761 gtgattagaa taacgtgaaa ataaattgtt gagcgtttat actgttttga aaaataaatt
     5821 taacatattg aaaaatacaa atatatacgt atattataaa gcgccgattg taaaattaag
     5881 ctagacaact aaaacagtca tttgtgctac actcaaaata gttccgacca aagaattata
     5941 aggagtgagt gcaaataacc tatacctatc ttacaacgga tgaacttgta ataatagagt
     6001 cttatttgaa aattaaccaa tctgttgcta aaactgcgca ttgcttgaat cgttcaagtc
     6061 aaacgatcca taaagtatat ctgttcttca agcaaagaaa atcagcctta gaata
//
LOCUS       DAJSAW010000097         6002 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJSAW010000097 DAJSAW010000000
VERSION     DAJSAW010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.098"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..395
                     /locus_tag="ONT64_002607"
     CDS             <1..395
                     /locus_tag="ONT64_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303984.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="ONT64_002608"
     CDS             398..1228
                     /locus_tag="ONT64_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HCR2303985.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="ONT64_002609"
     CDS             1244..2095
                     /locus_tag="ONT64_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2303986.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="ONT64_002610"
     CDS             2092..2451
                     /locus_tag="ONT64_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HCR2303987.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="ONT64_002611"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="ONT64_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="ONT64_002612"
     CDS             2624..2929
                     /locus_tag="ONT64_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303988.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="ONT64_002613"
     CDS             2929..3240
                     /locus_tag="ONT64_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HCR2303989.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="ONT64_002614"
     CDS             3237..3410
                     /locus_tag="ONT64_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303990.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="ONT64_002615"
     CDS             3407..3823
                     /locus_tag="ONT64_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HCR2303991.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="ONT64_002616"
     CDS             3823..4491
                     /locus_tag="ONT64_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCR2303992.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="ONT64_002617"
     CDS             4488..4865
                     /locus_tag="ONT64_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303993.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="ONT64_002618"
     CDS             4862..5161
                     /locus_tag="ONT64_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303994.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="ONT64_002619"
     CDS             5151..5309
                     /locus_tag="ONT64_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2303995.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="ONT64_002620"
     CDS             5403..5819
                     /locus_tag="ONT64_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCR2303996.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DAJSAW010000098         5912 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJSAW010000098 DAJSAW010000000
VERSION     DAJSAW010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.099"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            53..1060
                     /locus_tag="ONT64_002621"
     CDS             53..1060
                     /locus_tag="ONT64_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR2303997.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1072..2076
                     /gene="neuB"
                     /locus_tag="ONT64_002622"
     CDS             1072..2076
                     /gene="neuB"
                     /locus_tag="ONT64_002622"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HCR2303998.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2073..3221
                     /gene="neuC"
                     /locus_tag="ONT64_002623"
     CDS             2073..3221
                     /gene="neuC"
                     /locus_tag="ONT64_002623"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HCR2303999.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3194..3871
                     /locus_tag="ONT64_002624"
     CDS             3194..3871
                     /locus_tag="ONT64_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR2304000.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3861..5003
                     /locus_tag="ONT64_002625"
     CDS             3861..5003
                     /locus_tag="ONT64_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR2304001.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            5016..>5912
                     /locus_tag="ONT64_002626"
     CDS             5016..>5912
                     /locus_tag="ONT64_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2304002.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLL"
BASE COUNT         2194 a          754 c         1146 g         1818 t
ORIGIN      
        1 aaagattaag aatcgaatta tagatgacta taattcatag gaggagatat aagtgtttaa
       61 aggaaaaaca atattagtaa ctggcggaac tggttccatt ggaagtgaaa ttgttcgtca
      121 gcttttaaaa tatgaaccaa aagctatcag aatttatagt agaggtgaag aaaaacaatt
      181 ttatatgcaa caagagctct caaatcacac taatttgaga tttttaattg gtgatatcag
      241 agactctcaa aggattgatt atgcatgtcg tgatgtggat attatttttc atgctgccgc
      301 aatgaaacac gttccagcaa gtgaatataa tcctatggaa gctgtaaaaa caaatgtgat
      361 gggaactcaa aatgtaatag atgcagcgat caaaaataat gtaaaacatt ttgtaggtat
      421 cagtacagat aaagtagtga gtcctactaa caccatgggt gcgacaaagt tattgagtga
      481 aaaattaata atagctgcca atcagtataa aggaaaaaca gaaacaatat tttcttgtgt
      541 aaggtttgga aatgtcatgg ggtcaagtgg atctgttatt cctcttttta gtaaacaaat
      601 aaaaaacaat aaagatatta ctttgacaga tgttgatatg acacgattta tgatgtcaat
      661 accggatgct gcaagattag ttctgtctgc tgctatgaat tcaaaatttg gagaaacata
      721 tgtcttaaaa atgccggcaa tcaatatttt aactttagct caatcattaa taaaaatata
      781 taatgataca catgatgaaa aatatagagg tgaaataaaa gttgtaggta tcaggccagg
      841 agaaaaatta tatgaagagc taatgactac agacgaaatt gaacgctcat atgaaaatga
      901 taaaatgtat atcattccac cacactatca tgctactgac gaactgtaca aagattatga
      961 aaaaattaaa atcactgcat actcttcaga tgcaacagaa ttaatgagtg gacaggaatt
     1021 tattagaatc gcaaaagaga ataatgtcgt gacttggtaa ggagttttat gatgaaatca
     1081 gaagtatatg taattgcaga aattggtgta aaccataatg gaagtctaga tttagcttta
     1141 gaaagtattg ataaagctaa agaatgtgga gctaacgcgg ttaaatttca aacatttaaa
     1201 acgaataagc tagtttccaa aaaggctcca atggcaaaat atcagaagga taatcttaaa
     1261 gaggacagtt ctcaatttga aatgctaaaa aaattagaat tatctcaatc tgattttatt
     1321 agaataaaag attattgtat acaaaataaa attgattttt tgtctactcc ttttgatgaa
     1381 gaaagtgcag actttctaaa acaaattggt gtacaaggat ttaaaattgg ctcaggagat
     1441 ttaacaaatt taccttttct tagaaaaata gatatgtatg ggttgccagt tttattatct
     1501 acaggtatgg ctgatattca tgaggtcagt gaggctgtag attgtttcat agatagtcca
     1561 gttactattt tacattgtac ttcaaattat ccggctagtt ctgaagatat taatttattg
     1621 gcgattaaca cattaaaaga aatgttcggg atacccgttg ggtactccga tcattcattg
     1681 ggatatgatg ttggtatttg tgctgttgcg atgggtgcta aggtaattga gaaacatttt
     1741 accttggata aggaactacc tggtccagat cataaagctt ctttagatcc tcaggaattt
     1801 gcagattttg taaagcatat tagaaacact gaaatatttt taggtgatgg tgttaagcga
     1861 gcaatgcctt ctgaacttag tacaaaagaa gtatcaagaa aaagcctagt ggtcactgag
     1921 aatttaaatg tgggagatat tcttacagag aaatctctta caataaaacg tcctggaaat
     1981 ggaatagctc ctaagttttt atatcaatat attggcaaaa aagttaaaag accaataagt
     2041 gcagatagtg ttctgcttga ggaagatgtt gaatgaaaaa aatacttata gtaagtggaa
     2101 cacgagctga ttatggaata tataaacctg ttgctgagaa tttactacaa gataattggg
     2161 atgtatcatt tgttgtttct ggaatgcatt tatcccataa atatggattt actaaagaat
     2221 tgatcttagc tgacggattt agaattagtg gagaagttca aagtttgttt ttagagaaca
     2281 ataagggaaa catggctcgg tctgtggcat tagagataaa tggtttcaca aatatatttg
     2341 aacaaactag ccctgatttt gtattattac taggggatag aggagaaatg ttggcagctg
     2401 cgatagcttg tttgtatctt ggaatcaaaa cagcacatat tcacggaggc gaagtgtctg
     2461 gtactgtaga tgaatcaata agacatgcca taacgaaaat agcatctgtt cattttgctg
     2521 ctacggaaaa aagcaaagag cgtttaataa aaatggggga agattcttgg agagtttttc
     2581 ccgtcggagc tcctagaata gatactattt taaataatag attacctagt tttagagata
     2641 taaaagaaaa atatcatcta aaatttgaaa agaaaaaata cgttttgcaa gtatttcatc
     2701 cagttgtaac cgaactagaa aatatagaaa aacaaatttt agctttgatc aattcaatgg
     2761 aacaacaatc tcaaccaatt atttgcattt tacctaatag tgatgctggt agcgaaatta
     2821 tcagaaaaat gtaccagaaa tatttaaaaa cggatattat ttatattacg aatttagagc
     2881 cggaggaata cctgactata ttaaaagagt gcaaatttat gatcggaaat tcttcttctg
     2941 ggataattga agctgcatca ttcaaaatac cagtaataaa tttaggaacc agacaaaatg
     3001 gacgagaaca atctaaaaat attataaatg caaatctaaa tgaaaatgaa attgaaactg
     3061 ctttgaataa aatacaagat cctcagttta taaaaagctt gaaaaatgtt aagaacatct
     3121 acggtgacgg caacagttct agaagaataa caaaaacatt gaatcaaata ttgaatgatg
     3181 gggaagatat taagtggatt caaaaacaaa tagcttatta aaatatacta gtgatccata
     3241 cattattatt ggggctggcg gacacgcaaa agtcattatt gaaaccttaa gattagaaaa
     3301 taagttaata tatggatttt ttgatgataa gcaaaataaa atgcaagatc tgaaatatct
     3361 tgggaaaata aacgaattga atagctgggc ggatagcaaa aaaaaaatct ataaatttat
     3421 tgtagctatt ggtgactgtg tagtaagaaa aaagattata aaaaaattga atatgccaag
     3481 tgagcaatat ggaatagctg ttcatccaag tgctatagtg gcaaaagaaa tttcaattgg
     3541 ttatggaact gttatttttg cgaatgctgt aattaaccca gatgctgtaa ttggagagca
     3601 tgctataata aacacaggta gcattgttga gcatgattgt agaattaata actatgttca
     3661 tttatctcca ggtgtatgct tagcgggtgg agtacatgta ggtgtaggaa ctcaaatagg
     3721 aattggttct caatgtatcc agatgaagaa aattggatct tggtgtatga taggcgcagg
     3781 gagcactatt gttaaggata ttccctcaca ttccttagct tatggcaatc cagctaaaat
     3841 taagaaagaa ggaattaatt ttgaattcta gaattttatt atcaagtcct catatgggag
     3901 gaacagaaga aaaatatgtt gctaaggctt ttaaagataa ttggatagcg ccattaggtg
     3961 aaaatgtgga taaatttgaa gaaagtatta caaaatatac tggttctaag ggcggagctt
     4021 ctgcgttaag ttcaggtaca gctgccatac atcttgcctt aagactgctt aatgttggaa
     4081 aaaacgatat tgtctttgct ccttcattta cttttatagc tacagtaaat cctattttat
     4141 atcaaggggc tgtaccagtg ttcattgact cagaacctaa tacatggaat atgagccctg
     4201 ttgcactaga gagcgcatta gaagaagctg aaagaaacaa ccaattacct aaggcaatca
     4261 tcgtagtgaa tttatatggt caaatggctg attatgaacg aattttagct attgcaaacc
     4321 aatttaatgt tccagtgata gaagatgcag cggaatcgtt aggttctact tataagggaa
     4381 tttcgtcagg cttatttggg acaattggga tctattcatt taacggtaat aagatcatca
     4441 caacctcagg tggaggagct ctagtgtcta gtgataaaaa atatgttgag cattcaagat
     4501 ttttagcaac acaagcaaga gatcaagcac cgtattatca acatagcgaa ataggttata
     4561 attaccgtct aagcaatatt tctgccggta tcggacgtgg acaaatggaa gtattgaatg
     4621 atagagtagc taaacgaaga gaaattttca atcgatataa ggaagctttt tctggaattg
     4681 atagaattga aatgatgcca gaagtggatg gcatggtgcc aaatcgttgg ttgtctactt
     4741 taacgatcaa tgattataat tctattgatc aagtagttaa cgtaataaat aaactaaatg
     4801 aaaaaaacat tgaaacaaga gctttgtgga aaccactaca tttgcaacca gtatttcaag
     4861 gggtaaaaat gtattaccat gataataatc ttagtgtgtc agaaaagtta tttaatagcg
     4921 gcttatgctt accttctgga agtaatatga cagatagtga acaagaattt gttattgatg
     4981 agtttaaaaa aatattcaag taggtgtgat attttatgaa cataggaatt attggtttag
     5041 gttccatggg gaaaagaaga cttagactca ttcaagaaca ctttaatgat attttactat
     5101 gtggtgttga tactaatgag gcgagacgaa aggaagttga aaaggaattt ggaataccaa
     5161 cttataatag tctaacagag gcaaaaaatt tgtttgaaat aaattcagtt tttgtttgta
     5221 catcccctat aacccatgaa tttattataa aagaggcttt aaatttagat tgtaatatct
     5281 ttacggaaat caatttatta aatgaatatt atgatgaaat aatcgaagta gctaaaaaga
     5341 aagatagatt actttatcta tcttctacat ttttgaagag aaaagaaatt caatatatta
     5401 agtctaaagt gacgcataat aaaaaaattt cttatcaata ccatgtagga cagtatttac
     5461 ccgactggca tccttgggaa gattatcgca atttttttgt ttcaaacaaa aaaactaatg
     5521 ggtgtagaga aatattcgct attgaattac cttggattat agatacattt gggaaagtta
     5581 aatcgtttac tgtaatgacc cagaaattat ccaatcttga tattgattat tcggacagtt
     5641 tcactcttat tattgagcat gaaactggta tagttggaac tttaagtgta gatgttgttt
     5701 caagagtagc taaaagaaat ttaactataa ttggtgaaaa gattttaata gaatgggaag
     5761 gtaacccgga ttctctattt tcatggaatg aagaaacttc tagtatgaca gatattcaat
     5821 tgtacgatga ggttatgcat gataataatt attcacatac tattattgaa gatgcatatt
     5881 tagaggagat taaagaattt ttcacgcttt tg
//
LOCUS       DAJSAW010000099         5793 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJSAW010000099 DAJSAW010000000
VERSION     DAJSAW010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.101"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..988)
                     /locus_tag="ONT64_002627"
     CDS             complement(<1..988)
                     /locus_tag="ONT64_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HCR2304003.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGN"
     gene            1140..1835
                     /gene="vanR"
                     /locus_tag="ONT64_002628"
     CDS             1140..1835
                     /gene="vanR"
                     /locus_tag="ONT64_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HCR2304004.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1813..2967
                     /locus_tag="ONT64_002629"
     CDS             1813..2967
                     /locus_tag="ONT64_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCR2304005.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3182..4150
                     /locus_tag="ONT64_002630"
     CDS             3182..4150
                     /locus_tag="ONT64_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HCR2304006.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4143..5174
                     /gene="vanA"
                     /locus_tag="ONT64_002631"
     CDS             4143..5174
                     /gene="vanA"
                     /locus_tag="ONT64_002631"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HCR2304007.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5180..5788
                     /locus_tag="ONT64_002632"
     CDS             5180..5788
                     /locus_tag="ONT64_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HCR2304008.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1745 a         1131 c         1310 g         1607 t
ORIGIN      
        1 tatttccgat aaccgtgtat tgaaaggcat cttcgaagcc attatccaat atttcgcagg
       61 cttttgtata tttaggctgg tcgatatatt caccgactaa ggcattctta gctgttcgag
      121 cgagttcaat atccgtaaac ttaaagatcg cttttactgc ttctctaaac ggttttgaat
      181 tcttttttgg aatggaactg aagatgtttc ttaaaaaatg gacctggcat ctctgccaac
      241 ttgcgttggt aaatgactta cgaatcgcag acactaggcc tttatgggca tcagaaatga
      301 tgagttctgt cccctttagg ccgcgttctt ttaagtattc aaagaagatg gaccatgtgt
      361 catcactttc ttcattttga atcatgaagc caatgatttc acggtcgcca ccttctgtta
      421 tcccgatcgc aatatggcag cttttagaaa gcactcgatg gtcctcacgg acttttatgt
      481 agagaacatc agtcatcaga taaggataat tcgtacctga gagtgaacgg ttctgccatt
      541 cgttgaccat cgggtctaac tgctcagtca ggctagaaac aaaagatttc gatacagatt
      601 ttccacatag ctcttcaaca atctttgaaa ctttacgtgt cgaaacgccc gaaacataca
      661 tctcaagcat tgaagcgagc agtgcctttt catttcgctg ataacgctca aacaccgtcg
      721 gtgaaaattc accatcacgt gttctaggca cttttaattc gagtgtaccc acacgagtcg
      781 taaagtctcg ctcataatag ccatttcttt gactctgacg actttctgaa cgttcatagt
      841 catcggcttg aatatattct gttcgttgat tttccatcaa ttggttgaaa acagtggtta
      901 aaatattttt agaaacatca tcttttaccg aatgttcaat aatactttga acctcttcgt
      961 tgttcagtgt aaaatgtact tgggtcatgt aacgtcctcc tgggtatgtt tttgtggtta
     1021 aaaacattgt accgtaaaag ggctgttaca tggcctttta tcttttacac aattatatgg
     1081 actttatcct ataatctgat aaattattaa gggcaaactt atgtgaaagg gtgataacta
     1141 tgagcgataa aatacttatt gtggatgatg aacatgaaat tgccgatttg gttgaattat
     1201 acttaaaaaa cgagaattat acggttttca aatactatac cgccaaagaa gcattggaat
     1261 gtatagacaa gtctgagatt gaccttgcca tattggacat catgcttccc ggcacaagcg
     1321 gccttactat ctgtcaaaaa ataagggaca agcacaccta tccgattatc atgctgaccg
     1381 ggaaagatac agaggtagat aaaattacag ggttaacaat cggcgcggat gattatataa
     1441 cgaagccctt tcgcccactg gagttaattg ctcgggtaaa ggcccagttg cgccgataca
     1501 aaaaattcag tggagtaaag gagcagaacg aaaatgttat cgtccactcc ggccttgtca
     1561 ttaatgttaa cacccatgag tgttatctga acgagaagca gttatccctt actcccaccg
     1621 agttttcaat actgcgaatc ctctgtgaaa acaaggggaa tgtggttagc tccgagctgc
     1681 tatttcatga gatatggggc gacgaatatt tcagcaagag caacaacacc atcaccgtgc
     1741 atatccggca tttgcgcgaa aaaatgaacg acaccattga taatccgaaa tatataaaaa
     1801 cggtatgggg ggttggttat aaaattgaaa aataaaaaaa acgactattc caaactagaa
     1861 cgaaaacttt acatgtatat cgttgcaatt gttgtggtag caattgtatt cgtgttgtat
     1921 attcgttcaa tgatccgagg gaaacttggg gattggatct taagtatttt ggaaaacaaa
     1981 tatgacttaa atcacctgga cgcgatgaaa ttatatcaat attccatacg gaacaatata
     2041 gatatcttta tttatgtggc gattgtcatt agtattctta ttctatgtcg cgtcatgctt
     2101 tcaaaattcg caaaatactt tgacgagata aataccggca ttgatgtact tattcagaac
     2161 gaagataaac aaattgagct ttctgcggaa atggatgtta tggaacaaaa gctcaacaca
     2221 ttaaaacgga ctctggaaaa gcgagagcag gatgcaaagc tggccgaaca aagaaaaaat
     2281 gacgttgtta tgtacttggc gcacgatatt aaaacgcccc ttacatccat tatcggttat
     2341 ttgagcctgc ttgacgaggc tccagacatg ccggtagatc aaaaggcaaa gtatgtgcat
     2401 atcacgttgg acaaagcgta tcgactcgaa cagctaatcg acgagttttt tgagattaca
     2461 cggtataacc tacaaacgat aacgctaaca aaaacgcaca tagacctata ctatatgctg
     2521 gtgcagatga ccgatgaatt ttatcctcag ctttccgcac atggaaaaca ggcggttatt
     2581 cacgcccccg aggatctgac cgtgtccggc gaccctgata aactcgcgag agtctttaac
     2641 aacattttga aaaacgccgc tgcatacagt gaggataaca gcatcattga cattaccgcg
     2701 ggcctctccg gggatgtggt gtcaatcgaa ttcaagaaca ctggaagcat cccaaaagat
     2761 aagctagctg ccatatttga aaagttctat aggctggaca atgctcgttc ttccgatacg
     2821 ggtggcgcgg gacttggatt ggcgattgca aaagaaatta ttgttcagca tggagggcag
     2881 atttacgcgg aaagcaatga taactatacg acgtttaggg tagagcttcc agcgatgcca
     2941 gacttggttg ataaaaggag gtcctaagag atgtatataa ttttttagga aaatctcaag
     3001 gttatcttta ctttttctta ggaaattaac aatttaatat taagaaacgg ctcgttctta
     3061 cacggtagac ttaataccgt aagaacgagc cgttttcgtt cttcagagaa agatttgaca
     3121 agattaccat tggcatcccc gttttatttg gtgcctttca cagaaagggt tggtcttaat
     3181 tatgaataac atcggcatta ctgtttatgg atgtgagcag gatgaggcag atgcattcca
     3241 tgctctttcg cctcgctttg gcgttatggc aacgataatt aacgccaacg tgtcggaatc
     3301 caacgccaaa tccgcgcctt tcaatcaatg tatcagtgtg ggacataaat cagagatttc
     3361 cgcctctatt cttcttgcgc tgaagagagc cggtgtgaaa tatatttcta cccgaagcat
     3421 cggctgcaat catatagata caactgctgc taagagaatg ggcatcactg tcgacaatgt
     3481 ggcgtactcg ccggatagcg ttgccgatta tactatgatg ctaattctta tggcagtacg
     3541 caacgtaaaa tcgattgtgc gctctgtgga aaaacatgat ttcaggttgg acagcgaccg
     3601 tggcaaggta ctcagcgaca tgacagttgg tgtggtggga acgggccaga taggcaaagc
     3661 ggttattgag cggctgcgag gatttggatg taaagtgttg gcttatagtc gcagccgaag
     3721 tatagaggta aactatgtac cgtttgatga gttgctgcaa aatagcgata tcgttacgct
     3781 tcatgtgccg ctcaatacgg atacgcacta tattatcagc cacgaacaaa tacagagaat
     3841 gaagcaagga gcatttctta tcaatactgg gcgcggtcca cttgtagata cctatgagtt
     3901 ggttaaagca ttagaaaacg ggaaactggg cggtgccgca ttggatgtat tggaaggaga
     3961 ggaagagttt ttctactctg attgcaccca aaaaccaatt gataatcaat ttttacttaa
     4021 acttcaaaga atgcctaacg tgataatcac accgcatacg gcctattata ccgagcaagc
     4081 gttgcgtgat accgttgaaa aaaccattaa aaactgtttg gattttgaaa ggagacagga
     4141 gcatgaatag aataaaagtt gcaatactgt ttgggggttg ctcagaggag catgacgtat
     4201 cggtaaaatc tgcaatagag atagccgcta acattaataa agaaaaatac gagccgttat
     4261 acattggaat tacgaaatct ggtgtatgga aaatgtgcga aaaaccttgc gcggaatggg
     4321 aaaacgacaa ttgctattca gctgtactct cgccggataa aaaaatgcac ggattacttg
     4381 ttaaaaagaa ccatgaatat gaaatcaacc atgttgatgt agcattttca gctttgcatg
     4441 gcaagtcagg tgaagatgga tccatacaag gtctgtttga attgtccggt atcccttttg
     4501 taggctgcga tattcaaagc tcagcaattt gtatggacaa atcgttgaca tacatcgttg
     4561 cgaaaaatgc tgggatagct actcccgcct tttgggttat taataaagat gataggccgg
     4621 tggcagctac gtttacctat cctgtttttg ttaagccggc gcgttcaggc tcatccttcg
     4681 gtgtgaaaaa agtcaatagc gcggacgaat tggactacgc aattgaatcg gcaagacaat
     4741 atgacagcaa aatcttaatt gagcaggctg tttcgggctg tgaggtcggt tgtgcggtat
     4801 tgggaaacag tgccgcgtta gttgttggcg aggtggacca aatcaggctg cagtacggaa
     4861 tctttcgtat tcatcaggaa gtcgagccgg aaaaaggctc tgaaaacgca gttataaccg
     4921 ttcccgcaga cctttcagca gaggagcgag gacggataca ggaaacggca aaaaaaatat
     4981 ataaagcgct cggctgtaga ggtctagccc gtgtggatat gtttttacaa gataacggcc
     5041 gcattgtact gaacgaagtc aatactctgc ccggtttcac gtcatacagt cgttatcccc
     5101 gtatgatggc cgctgcaggt attgcacttc ccgaactgat tgaccgcttg atcgtattag
     5161 cgttaaaggg gtgataagca tggaaatagg atttactttt ttagatgaaa tagtacacgg
     5221 tgttcgttgg gacgctaaat atgccacttg ggataatttc accggaaaac cggttgacgg
     5281 ttatgaagta aatcgcattg tagggacata cgagttggct gaatcgcttt tgaaggcaaa
     5341 agaactggct gctacccaag ggtacggatt gcttctatgg gacggttacc gtcctaagcg
     5401 tgctgtaaac tgttttatgc aatgggctgc acagccggaa aataacctga caaaggaaag
     5461 ttattatccc aatattgacc gaactgagat gatttcaaaa ggatacgtgg cttcaaaatc
     5521 aagccatagc cgcggcagtg ccattgatct tacgctttat cgattagaca cgggtgagct
     5581 tgtaccaatg gggagccgat ttgattttat ggatgaacgc tctcatcatg cggcaaatgg
     5641 aatatcatgc aatgaagcgc aaaatcgcag acgtttgcgc tccatcatgg aaaacagtgg
     5701 gtttgaagca tatagcctcg aatggtggca ctatgtatta agagacgaac cataccccaa
     5761 tagctatttt gatttccccg ttaaataaac ttt
//
LOCUS       DAJSAW010000100         5790 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJSAW010000100 DAJSAW010000000
VERSION     DAJSAW010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.102"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(99..1229)
                     /locus_tag="ONT64_002633"
     CDS             complement(99..1229)
                     /locus_tag="ONT64_002633"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR2304009.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="ONT64_002634"
     CDS             complement(1877..3079)
                     /locus_tag="ONT64_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2304010.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="ONT64_002635"
     CDS             complement(3139..3342)
                     /locus_tag="ONT64_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HCR2304011.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="ONT64_002636"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="ONT64_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="ONT64_002637"
     CDS             4879..5691
                     /locus_tag="ONT64_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2304012.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DAJSAW010000101         5341 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJSAW010000101 DAJSAW010000000
VERSION     DAJSAW010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.103"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(61..279)
                     /locus_tag="ONT64_002638"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="ONT64_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="ONT64_002639"
     CDS             complement(545..739)
                     /locus_tag="ONT64_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304013.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(839..925)
                     /locus_tag="ONT64_002640"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="ONT64_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2091..3131
                     /locus_tag="ONT64_002641"
     CDS             2091..3131
                     /locus_tag="ONT64_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR2304014.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            3955..4287
                     /locus_tag="ONT64_002642"
     CDS             3955..4287
                     /locus_tag="ONT64_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCR2304015.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4906..5247
                     /locus_tag="ONT64_002643"
     CDS             4906..5247
                     /locus_tag="ONT64_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HCR2304016.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1943 a          728 c          900 g         1770 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag
     1501 gtagtatcta atttaagtat taatggtagt atctaaatta acaggtagta tgtaactact
     1561 ttaaagtaat ttttttcaga ttaacacagt agtatcttat taaataagtt aattttaatg
     1621 gtagtacata attattatat atatctgaca ttggtagtac ttattgtatt ttttagtagt
     1681 atataatcta ttgttttcta gcctaaagcc ttgtcttata agaataatat aatatatact
     1741 actgaaataa ggtagtatat attactagta atggtagtat gttttatatg cataaggtag
     1801 ttcttaattt attttttgtt ttgacactgg tagtacttaa ttatctttaa ttttaaaact
     1861 aaattttatt catacgtttt tctggtagta tttaattata aataatattt atttaaagta
     1921 gttcttaatg actttaaagg tagtacataa ttcattttat ttctgatttt aggtagtatt
     1981 taattaaaaa acagttttaa tgcgtttgca ttttttattt atctttgcta ttttcgaaaa
     2041 gagtaggtaa ttttacataa ataaaacaac gtgaaagtag aggatctaca atgactgatt
     2101 ttaaattttt taaggcggat agagtttaca acgaattatt ttatcaattt ccaaaagtct
     2161 ttattgtttc tgacgaatac aaaaaaatga aagattcaac taagattgcc tatatgcttt
     2221 taaaagcaag attagagatc gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt
     2281 attttactta tacgacaaat gaactatgta gagtattaaa ctgccaaaaa caaaaagcga
     2341 tagcaatcaa aaaagagttg gaatcctttg gtttattatt acaaaagcag atgggattta
     2401 acaaacagtt agggaaaaat aatcctaata gactatatct agcagaatta aaagtctcag
     2461 aaaatgatat ctacttactc gaaaaatttg atagagagaa tagggaaaac gttgataaat
     2521 cagagggtat gaaaatcata cccaccctcg acgaaaaatc agacgctgaa tcccttgggg
     2581 ctcaagaggg tatgaaaatc ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa
     2641 tcataccaga acttaataat aatatattag acactaatag acacaatata gacactgaaa
     2701 aagaccgcct acaagatcaa ttgttgttag acaattttga gacaattatg acaaacgaca
     2761 gcattgctac gtttgtccct gaacgagtat taaatttgat aaaaacattt tcttcaagtt
     2821 acagtgaagc tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa gctgaaatag
     2881 aaagtggtat ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat gcagaacaag
     2941 aattatacac gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata
     3001 tccagaacct gatttttgtc tatgtaaaaa attggtttat cgaaaaacca atagctgcta
     3061 aagtatcaag tgaaaaacgt ttgaattatg aaagctcccc aagcactatt acgaaagact
     3121 ggttagagtg aaactttact gacaaagtga ttgttgcttc atggagtaac attaaaatta
     3181 aaaatttttt attattctaa ttatattacg aatattacaa aataatagct attttaatag
     3241 aatttataag ttaaaatatt atgataaaaa tcacaaacgt agccttttac gtcataagtg
     3301 ttaaaagaaa taattaattt caaatataga aaagggaacg tatgtttgca tactcctttt
     3361 ttttgagtta taataatacg cgaagagaga taatttcatt atttaaacaa gctaaaaatc
     3421 ttgatattac ttcttttcag gcaatacgaa tattattcgc ttacattttt tgtataaatt
     3481 taattgtctg ctatgcttgt gatgaattga ttttcgttct gcacttttgg atgataaatt
     3541 tggacaattg aaaatacaaa aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt
     3601 cgtcagcgtt catacacggc attaagattt ggcccttaat gcgttaacct ttcagtgtta
     3661 cggtattacc cgcaatggat ttagtataac agatattcaa aagtgatacc actttttttg
     3721 gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc tatgcctaag cacaatgtta
     3781 taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg
     3841 tttggatgcg ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa
     3901 aaataacaag aaaaacttgt taaaaatatt taaagaaatt gggtggttta ttttatgtca
     3961 tatacaggaa ttttgagtct caaagatatt tgtcactacg gtaaacggtg taccgctaca
     4021 gagaaaatca cgaagaaact atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa
     4081 tatataatcc agaaagacaa agtctcagag gaaatgattt attacattgg gaaacaaaag
     4141 caaataattt taaaagatcc tatcccctta aaagagctat atccaacaat caaacatgtt
     4201 tatgaccaaa acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca
     4261 aaagggatgg ggcgtttgat cggctgaatt gatcattctc ataacttgta ttttatcgtt
     4321 tttcgcttgc ttgaaaatat tgtcgagact tctaaaggaa cttagagtca ctagaaaaat
     4381 aaggagtaga cttgatctat caacaattcc cttcggggag tgacggggta ggttttaacg
     4441 attggtaacg taaaaaattg gtcaatcgag taaatgcgct tgtttggata gtggtgaaat
     4501 gaatttgtgt aaataataag cgcagagtaa acaagaggga atttagaagt tagacaagtt
     4561 agaatagcgt ctagattaat catgagcagc tactaaggga tctagacttt gacgcaaact
     4621 catggaataa ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt
     4681 gctgcagtac atgtagatac ttgcgaggac gttgattatt ggatcaatcg agcatttacc
     4741 gctgtctact agggtttctc gtttcggacg ccataaacga gtcgaaattt tttatgtatt
     4801 tttaagtaaa cgaaaaacga taaaatatga acaagtcctg aataaagtga aaaaattgat
     4861 ataaaacata gagaaaatga aatttacgta atggaggaat gacaaatgaa aaattatgat
     4921 ccgaatatac gattgggcac acatactatt aaggtttctt tccaacgttg ggattacaaa
     4981 ggatttgtga cgtttagaag aggcggaaat tgtaaaggat tagatgtttt agcattagac
     5041 gaggatgatt tatacgatca aacattgact gataatccaa ttggattcgg cttgctacct
     5101 gaagatgatg aaggtaatga atggtttaaa atgacgctta tgaatgataa tggagacgaa
     5161 ttatctgttg aagatacttg gtcatatttg agtgattata ttgtcagctt tgaaatcatt
     5221 gaatttgtag cggataaaga agaataaaaa ctggaggaat aaattttaca gataaaggct
     5281 tagaaggagt cacgatgatt aataaaaagg tagtattacc ttaggcattg agtaaggaat
     5341 t
//
LOCUS       DAJSAW010000102         5250 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJSAW010000102 DAJSAW010000000
VERSION     DAJSAW010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.104"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1666)
                     /locus_tag="ONT64_002644"
     CDS             complement(<1..1666)
                     /locus_tag="ONT64_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCR2304017.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQN"
     gene            complement(1708..2766)
                     /locus_tag="ONT64_002645"
     CDS             complement(1708..2766)
                     /locus_tag="ONT64_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HCR2304018.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(2885..3340)
                     /locus_tag="ONT64_002646"
     CDS             complement(2885..3340)
                     /locus_tag="ONT64_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304019.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(3924..4727)
                     /locus_tag="ONT64_002647"
     CDS             complement(3924..4727)
                     /locus_tag="ONT64_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR2304020.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1551 a         1026 c          727 g         1946 t
ORIGIN      
        1 aattttgacc atatactctt tctttcctag gtggaagacg taaatctata tttaaatgtt
       61 ttcgtactaa ttcagcactc ttcaacattt cattataagc aatttccaga agagtttgtt
      121 cggataaagc ccaaaatgga actattcgtc caaatcggtt aataacttca ggctttagac
      181 cttcaccaat caaggcttgt ttgatccctg atgcgacttt tcggtattct ctttttagtt
      241 cagtgctgta ttcattagaa ttttccttgt ttgtaacacg agatagtgag ctatatcggt
      301 ttaacgaatc gaacatctcc gcacctgcat ttgttgtcgc aaataaaatt gtatttgaca
      361 atgaaacggt gtcgtgaact tctcgatcat cccatcctct ttcagtaaaa cttatcctac
      421 cttcatctaa aattgataaa aaggcatcga gtgtttctac accagctttc tcatattcat
      481 cacacacaat aatcgcagaa ggatcatgcc ttacatgagt acctataagt cgtctaaaac
      541 gcattgcatc atctttggtt ttgtagtccg gcatattaac taagagaact ttattttcat
      601 cgccataaag tcctcttgcc acggctttag ccattgcagt tttaccgacc cctgttgatc
      661 ctgtaaacag agcagttgct tttggttgct ttcgaccttt aaaacgatca aatccaagct
      721 tcatacgttt aaacaacatt gttatagtat aaactgctaa cggttgtcct tttatggact
      781 ttaattcctc ttgaagtttt tccatatcaa aatcagaatc tagctgaata ttatacttac
      841 caaatatttg caatagactt tctcgattat aagcatttcc gtctactcga tcttttgcaa
      901 tcaaatcctc cattacagca attgatttag ccggttcgga caaatcctca cgataaagtt
      961 tgtttgcttg tacgatttct tgaagtaatt catcaggaat cctttgcgat aatctaaaag
     1021 aagcctgagg atcatcttta acagaggatg tatttagcca ttgtctcaaa atatcgacga
     1081 caacatcgct ttttagttca ttcaattcta acggttgtag ccgtctccct aaggcatcat
     1141 ctcttttgat atagttttca tattcttttc ttgtagtagc ggtaatcatt tgaagtgatt
     1201 catttgctaa cggttctttt agcgaatcac ccccaatttt attgccactc ccaaaagagc
     1261 taacaacttt atgaaactcg tcaatgaaga ggacaaattc attatctttg tcctcagctt
     1321 ttaaaattcg ctcatattct ttcaatcttg gaattaatgt ttctaaacgt gtttggagga
     1381 catcatctcc cccaacacgc aattgtccga ttgataattt atatacttgt actttctttc
     1441 catcttgtcg ctgtctttca gcccattcac ttgcaagtgc tgttttacct gttccggctt
     1501 cacctaacaa gatagcttgc ggtgtttccg gtctattcaa gatcagtgac aactcatgga
     1561 gttcgtcatc acgtcccact aacttatgtt ttggtggttt cattggtgtg agagcatgat
     1621 taaattcggt ttgaatattc ttaaaaaact cggtactatt ttccatctgt ttgtgttcac
     1681 ttcctctaaa ataagaataa tttacttcta gtcagtcaaa gttaggtact taaatgtcgt
     1741 cttcatgtct ccaaaagaat ctttgttaaa gtcagcattt ttgtattgat caatcaattt
     1801 ttgtgtatca ttgattgctt taatttcggt ctccattcga ctgtctagtt gacgaatact
     1861 ttcttgcgaa tctttatcgt caggattttg ttgtaaacgt tccagatact cagcttttgt
     1921 ggcttcaatt ttcttgatgt tagtatttga agcatttaac tgatcttcta gcttatttac
     1981 aacattattc ttacttgtaa ctgctacaac ttttgaataa tcaatatcgc catctttggt
     2041 aagaaagctt ccttgataga cattagttga atctgaatat ggatttattc tgaaattgat
     2101 attatcagga gtattaacag gttttgcttt tttcccaaaa gaaattactc cttcattatt
     2161 aacatcattc atagaatatt gagaaagtgc ttttgtaact gaatctgctt cattcacatt
     2221 gactttggta tcatcgtcag ctgtatcatc accagaaatt agtgatactt ggtttgcaat
     2281 aaaaacttga taagctcttt cctctaattt accttttagg aataagaaag catccccatc
     2341 agtacttaac atcccatagc tcatttcaag cttcttagga cgatcttttt cttttgttgc
     2401 catatataag ttatagtttt ttccttgcgt agataacaac tttctagcgt tttgggagta
     2461 accaagtttt actactgtta catctctatt ttgatctgtc cacacatctg aaatagtgat
     2521 ttctgctcct gatcgagcaa actccaatga agaccctata ggtgttgtat tgttcacgag
     2581 ataataatta tagtttccta attgataaat gtagctaccc aagcctgtta ataaaaaaag
     2641 cactcctaga aaagttgcaa gcataatgcg taatccgtga cgtgttctct tatccttatc
     2701 accaagcaac cctccaataa tactgtattg atctttggta gatgttgaat tgacgttttt
     2761 ttccatcagt ttgtctgccc cctttcataa aagagcaaac aatcagacgt tttttcggta
     2821 attctaggtt cgggattttg aatcatttcg gtatattctt ccccattaat atttaacaca
     2881 aaaattatct tcctttcttc gttcctttct tttgctcttt attttccaaa taatcaatga
     2941 tttttgctat tgtaccaaga tagtaattcc cctcagcttt gttttttcgg attttgctaa
     3001 ccattggagt aggtacaatc tcttcatctc tcaattcact gaaagatttt ttacctagat
     3061 attttccaat aacagatttt aaaaaacgtt caaaatcttt ctcgtaagtt gttttcttgc
     3121 cttttagttc atggttcata ttgtcaacga cttttgcggt ttcaataatt gttttagttc
     3181 tcagcttaat aatttcatct ggaattttca atcctcgtaa tttcatcgtt gtactaactg
     3241 gtaacccatg ctttactgca tcagcaatac taaaactttt tagatcatct agtaagctct
     3301 ccatatagtt aacaaattca tgccaattaa tagcattcat tgtctcaccc cttcattttt
     3361 ttaaaatgag gttcgggcgt aacacattta tgttattata atatcataat aacataaata
     3421 aatcaatcag caaatttaga tatcccttaa agtatcagca attattcaac aagatattta
     3481 ttttagctat ttcagtcatc ataaaagact aattagcaat aaaataacta atattatcca
     3541 tttatcagac gaaaagaaaa aacttatcta attactctat attaactttt aacaaatagt
     3601 tgacttgtcc aaaaatgtta ccttcaatct cacaaagata ttaagatgtt tcctaccaaa
     3661 actgatatca ccggactttt tattctgtgt tgtccaaaag tgttaccttt agtcgatcaa
     3721 caaattaaaa aaaattgttt ttttcttgac ttgtccaaaa gtgttaccca tagttgttaa
     3781 aatttgtata gattagccat tctttcgaat attatttaga ctgaaacttg cttacaaaaa
     3841 aacttcttgc atttattgtc caaaagtgtt acccatactt ttttaaagaa gcaggttgta
     3901 ttaaaattac aacctgcttc ttctcaatct tcttctaacc aattatgcat tgaaatggtt
     3961 ggcaagtttg attctggctc actaaaagtg aatctgagcc gatcaactct tcttcctttc
     4021 tttgcataga ttttttcata atcaaaagtt tcaaatacct gtattccgtt ttcatcagga
     4081 gctaaaaatt cttctttcgc ctgctctagt attcttttgt caatatttgt cattctataa
     4141 cttgaaggta tacttaatgc tcttctaaaa tcttcaactt tgactaccca ataaccattt
     4201 cgccactctt tatttctgta ttgcatgaga tgacgataca attcttttgt gtagctactt
     4261 cttagttttg taaaattatc aagttcaaaa cgagtaaagt ttttgccaat tgagtttaag
     4321 ataaattcaa attcctcatt tgccttaatt gtaactgttt ccttctgttt atctatccgg
     4381 aatttttgaa aaagccacag ctgttcatag acgtctgaat cttcaaaacg gtaggttaaa
     4441 gtacctagtt tgtcactcaa agcttttaac acagaataaa aactgtcctt ttctcttctg
     4501 tcgtatttga ccaaataacg aaagaggttg aaatcaaaag ttacttctgc tgttccttga
     4561 cgtttcattt ttgataaaat agcccaaaac acatccattt caacaggctt aaatcctcgc
     4621 aaagctgttg tattcagacc attgttataa cgcacaattg catttgtttg ttctgatact
     4681 aaatctacat cactatcaat aatcttttcg gtaacttctt gagacattga ttactctcca
     4741 atcttctttt tgcgctcctt taatcatgtt aatctggtac taattaaagg aaacaatttt
     4801 ttctgcttgt tacctaaatt aaatcacaac taaaggtaac agtcaacaaa gatttgttac
     4861 taatctaatt ttctattttt tcgccttcca aatcaagaaa accgaaggta acgtcttatc
     4921 ctagtgtgat acctcaaatg tccacttttg ttacctttgc tgtccaaaag tgttaccctt
     4981 actgtccact tttgttacct ttactgtcca aaagtgttac ctttataccc ctttaatact
     5041 tactctccca agagattaag cctcccctaa cagatatatt aacagatatt taacagatat
     5101 attaacagat atttaataaa ggaggattac tcctctctta tttcaaaatc ttaaaaataa
     5161 aaaatcttaa aaaaaactaa cactatctag gagtttattt tgcttgtgta actttgaata
     5221 attgaaaaaa aggaaaagga ggaagcttca
//
LOCUS       DAJSAW010000103         5212 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJSAW010000103 DAJSAW010000000
VERSION     DAJSAW010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.105"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            604..954
                     /locus_tag="ONT64_002648"
                     /pseudo
     CDS             604..954
                     /locus_tag="ONT64_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
     gene            948..1370
                     /locus_tag="ONT64_002649"
     CDS             948..1370
                     /locus_tag="ONT64_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304021.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2540..3508
                     /locus_tag="ONT64_002650"
     CDS             2540..3508
                     /locus_tag="ONT64_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR2304022.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3525..4049
                     /locus_tag="ONT64_002651"
     CDS             3525..4049
                     /locus_tag="ONT64_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCR2304023.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTETHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKTEKKWWQFWQ"
     gene            4425..4706
                     /locus_tag="ONT64_002652"
     CDS             4425..4706
                     /locus_tag="ONT64_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304024.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4708..5124
                     /locus_tag="ONT64_002653"
     CDS             4708..5124
                     /locus_tag="ONT64_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304025.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1967 a          893 c          868 g         1484 t
ORIGIN      
        1 aaaaaaaaga aaaatccgct tatgcaactt cttctttttg tattttgggg cgctacccca
       61 atccccaagt atcggttatt taatagcgcg gaaatccaaa gaataggacg taccctgaaa
      121 caaaaaaaga ctgctctcac gagcagccca aaatgagatt atcctattca tcaacataaa
      181 aatattacaa agtgaagaac tagagaaaga caaaaaaagc atatcaaatc ctctcaaaaa
      241 taacaagtct taaaaaaatg acagataaaa atgcacaaag aattagatat agataaccgc
      301 gaaaaatcaa gctttagaaa gttgctttta gctagaccaa ttaaaaatca caaagtgagc
      361 gtctctatcc attggtagag gggctacttg gcatagtgta cattatgtta actagctcga
      421 taaataacga ttatgttccc aaaacaatat tttatgaact ataacaaaaa tttgttatcg
      481 catattgtat attttaatta tataattata ggtgtatacc acttaaattt aacacaaagg
      541 cgtggttatt ttgaaaccca aaaagaaaat ttatctaggt ctcctctttt ccttgatgat
      601 tggaatagtc aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat
      661 tactgtggtg tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca
      721 caactaagaa aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac
      781 agccgtcatg cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat
      841 gtgtggaatc cttggtataa ctacccaact gtaataagtg caaatgcagg accagttaca
      901 attcctgtac ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac
      961 ccattgcttt agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac
     1021 aagaaataca aaagactgat ctgattcctt ttctaggaac ttggcaaact aaagatcatt
     1081 cagaacgtct agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct
     1141 tagtaaaaga gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac
     1201 aagttttcat ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat
     1261 tagattacac ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg
     1321 atgggacaga agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg
     1381 tttgatatta caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt
     1441 ggctagcatt tgctagcctt ttttgtattg tggagcgtgg tttttgaggg ggattcaagg
     1501 gggagcttgc gcccctttga caagcggtta tttgcaccaa atacgtaggt atttggctag
     1561 caaatataga gcttgccaaa cactttagtg agttttgtag aaagtacctt taaaggtact
     1621 ttcacattac gaacgatacg tccgtaggac gtactgaaag ggtgggattt atgagcgaac
     1681 taatgcaaaa tttggctagc aaaaaaagtt aaaaaatata tgctaataat cgagcgaaat
     1741 gaacactttt tcaagtgagc gaatccgcca tgacaaagct gaagcagttt atttcagctt
     1801 tgtcatggcg tgtgcctaga cttttaaaaa agtcggcaca cgagcaaata tctttatcaa
     1861 gctctaattt gcgttttaag acgtttgaaa cgtctaagta tataaatata taagagtagg
     1921 tttaagagtg cttaaatcga attttagagg gcattcagac aactttaggg ttgttagggc
     1981 ttgccctgac cgcctgtaag gcgctttaag aacaagataa gggatttata ctaagactgt
     2041 cttaaaacgt ctcaaacgaa ggctcatagc ttctcaaaga gttccttatg aacattttta
     2101 gcaaaggagc cggaagcgac tgcgcagtat aatgtgagag cagagcgatc acaccttttt
     2161 aagcgactgt agggagtaag attcccagtt gagaaaaatg cgcgaatctg tttggagaaa
     2221 aaagaagtgg ttttttaggc gggaaatttt gtccccctct tttataatct ctttttaaac
     2281 ctctttttaa acctctttac cgtcggctat atccttactc tcccaaggga atgacgcttt
     2341 tatctacatc gttttgtctg tttatctaca tcgttttgtc tgtttatcta catcgttttg
     2401 tctgtttatc tacatcgttt tgtctgtttg tacattgaat tagtacatcg acgatgttaa
     2461 tataagatgt agataaacag gcattttgta tttcataacg tgtatctctt gtgcgagtaa
     2521 gaaaggagct attttaaata tgtctaatga aatcgtaaag tatcatcatg aattaaatac
     2581 aattccgttg cgaaaattta caccagtcga aatgaatttg ttcttttcta tcgtatcaag
     2641 aatgcgtgat gtaggtgata aaaaagtaca atttacattc gagcaattaa aagatttaag
     2701 caattataaa gcaactgcaa atgttcgatt catagatgat ttagaaacaa cgtatgacaa
     2761 attaatggat ttacgttttg gtagaagaag tgctgacggt ttgcaacgtg aacgttttgt
     2821 tttatttaat caatttaaaa tcgacggaaa atctgatatt ccttttgctg aaatacaagt
     2881 tcatgaaaaa gccttaccct tacttaataa tcttgaagaa tgggtgcgct attctctcca
     2941 acaattcaat gaattagaaa gcagttattc aaaaacaatg tttcgattac taaaacaata
     3001 cagaaccaaa ggtttcgctt atttttcaaa agaagacttt catgaattac ttgatattcc
     3061 taaaagttac aaccaaggta cgattgatca acgtgtgttc aatccaatac gacaagaact
     3121 aaccgcaatc tttaaaggtt taacaatcaa aaagaaatac ggaaaaggac ggggaaagcc
     3181 tgtcattggc tatcaattta catttaaacc tgagataaaa aaatcagacg actttaacaa
     3241 acatgcccca acaaaagaag aacaaaacaa agcattagaa ctagctaacc aacaagttca
     3301 atctgaaaaa gcacctactt cattacaaga aaaaatcaaa gaacaaaagc gaaaattgga
     3361 acttttacaa cagttagaaa agttagaaag agaacaaaat acccaaaata aagaaaataa
     3421 tgcacaaaaa tcagagataa attccgatat tccttcagaa ctaaaaaaag acttattaga
     3481 caatcttcaa aacttattca aagattgagg ggtttttaga ttttatgaca gaaaaaacaa
     3541 tcaaagaact tgctgaagaa ttaggagtta gtaaaacagc aattaacaaa aaagtttctg
     3601 acgaaaatcg aaaactttgg ttttcgaaaa tcggaaacaa gtttgtgatt aatgaaaccg
     3661 gacaaaaggc tataaaatca atgtttttta acgaaaacga aaactcaaat caacaactag
     3721 ttggcgaaaa aacgaaaacc gaaacccaca aaaacgccaa ccaagttccc gaattagaag
     3781 tcctttctct cctagaacaa cagttaaatc aaaaagatat gcaactacaa gaaaaagacg
     3841 agcaaataaa acaaatgcaa aaattactcg accaccaaca acaactcacc ctgcaagcca
     3901 accaacaaat cgagaaactg caggaacaac tacagttgac ttatacggaa gaatcttcgg
     3961 aaaataaatc aacgactctt tctgaagaaa cagaatcgat agaaaaacaa tcaaagacgg
     4021 agaaaaaatg gtggcaattc tggcaataaa accagcaatt taaattaata aaaattgctg
     4081 gttttattct tctttttgct tttgcgagca gagcaacatg gacccatgtt gcgatatttt
     4141 ggtggcgccc caatccccgt tttttcgttg aatcttactc ggactgcact cgttttacag
     4201 ctgatttttc tgaattttga aagagcataa ggaaacgtgg tatcactttc ttacgaaagg
     4261 tgctagatag gctgtgccta tcacgtttct cagaaaaaaa tttgctatca cttttttgcc
     4321 attgtggcta ggctatgtat agtgttttgg cgaaaaatag ctatacaaat actatatttg
     4381 tatggtatac ataaattata cgctgatacg aaaggaaaat ttccatggac attttagttc
     4441 gaaatatcaa tcccaaaatt attgcaagaa tagataaacg ttgtcaggaa ctcacaacca
     4501 aaacgggcaa aaaatggagt cgaaataaat atctaaaagt tctaatcgaa agtgattttg
     4561 accgtcaact tatgaattac aaaaaagatc aattcgattt attactcgaa aaatttattg
     4621 ccattcaaac agaaaatacg aacatactga atgactatgt tcacacaaac aaccaactca
     4681 ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa acacgtaacc
     4741 ctttatccca gtcacgttga cgttttagat tccatcatcg aaaaaaatcc agacgtcaac
     4801 aattattcag aagccattcg ttttttatgt atgagttatg aacctgatac caatacaacc
     4861 acaaccagta accaagcaaa actcaatgcc atgggaaaag aaatttctat catgacagaa
     4921 atcatggcag aatttgctga tttatatcta aaagatactg gtgttcttat cggtacaaat
     4981 tctaaagtgt atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc ttcccgacga
     5041 aatcaaccca aaaatcgtca aaaagaaacc aatcaaacct ttacttctgg aaattttgac
     5101 ccaacaaact ttctaaaaag ataaaaaaag aaaccgatcc ctaaaaaaat tcctcgattt
     5161 ctttttaggg atcagtcttc cagcaagatt tgcaagttgg taccaacttg cg
//
LOCUS       DAJSAW010000104         5199 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJSAW010000104 DAJSAW010000000
VERSION     DAJSAW010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.106"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            25..696
                     /locus_tag="ONT64_002654"
     CDS             25..696
                     /locus_tag="ONT64_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR2304026.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="ONT64_002655"
     CDS             755..1195
                     /locus_tag="ONT64_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304027.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="ONT64_002656"
     CDS             1207..1875
                     /locus_tag="ONT64_002656"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR2304028.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="ONT64_002657"
     CDS             1939..2562
                     /locus_tag="ONT64_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HCR2304029.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="ONT64_002658"
     CDS             2618..3004
                     /locus_tag="ONT64_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HCR2304030.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="ONT64_002659"
     CDS             3217..4608
                     /locus_tag="ONT64_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCR2304031.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1728 a          861 c         1095 g         1515 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacag
//
LOCUS       DAJSAW010000105         5015 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJSAW010000105 DAJSAW010000000
VERSION     DAJSAW010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.107"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            286..1236
                     /locus_tag="ONT64_002660"
     CDS             286..1236
                     /locus_tag="ONT64_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HCR2304032.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            1257..1790
                     /locus_tag="ONT64_002661"
     CDS             1257..1790
                     /locus_tag="ONT64_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCR2304033.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            1806..1976
                     /locus_tag="ONT64_002662"
     CDS             1806..1976
                     /locus_tag="ONT64_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304034.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            2426..2959
                     /locus_tag="ONT64_002663"
     CDS             2426..2959
                     /locus_tag="ONT64_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304035.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            2971..3174
                     /locus_tag="ONT64_002664"
     CDS             2971..3174
                     /locus_tag="ONT64_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304036.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKSKKK"
     gene            3496..3876
                     /locus_tag="ONT64_002665"
     CDS             3496..3876
                     /locus_tag="ONT64_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR2304037.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            3858..4772
                     /locus_tag="ONT64_002666"
     CDS             3858..4772
                     /locus_tag="ONT64_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR2304038.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
BASE COUNT         1874 a          880 c          917 g         1344 t
ORIGIN      
        1 tttgtcaccc tcttttataa tctcttttta aacctttttt taaacctctt taccgtcggc
       61 tgtgtcctta ctccttcaat ggatacagcg ttttataaac tacattttgt ctgcttttaa
      121 actacatttt gtctgctttt aaactacatt ttgtctgctt ttaaactaca ttttgtctgc
      181 ttttaaactc tttttttgca ctcacgtatg ttataatatg cattatcaca gacaaaacgt
      241 tgtttataac gtctgtatcc attgaaggag taaggaggaa tatctttggc aaatgaaatc
      301 gtgaaatacc atcatgaatt aaataccatt ccgttgcgga aatttacacc agttgaaatg
      361 aatctatttt tctctattgt gtctagaatg cgcgaaaaag gcgataaaac agtccgtctt
      421 acttttgacc aactaaaaga tttaagtaat tataaagcaa ctgctaataa tcgtttcatt
      481 aatgatttag aaatgactta tgataaatta atggatttac gttttggtaa aagaagtgct
      541 aatgggctcg atatagatag attcgttatg tttagtgagt ttaaaatacg tggaactgct
      601 aaagatccat atgtagacat acaagtttac gaaaaagctt tacctttact aaacaattta
      661 gatgagtggg ttcgctattc actccaacaa tttaacgaat tacaaagtag ctatgcaaag
      721 actatgtttc gattactaaa acaatatcga actaaaggtt ttgcttattt ttctaaagag
      781 gattttcatg aactattaga tattccaaaa agttactgga acaaccaagc aaacgttgat
      841 aatcgtgtta taaagcctat acgacaggaa ttaaccgcaa tcttcaaagg attatcaatc
      901 aaaaagaaat acggaaaagg gcgaggaaaa cctgtcgttg gctatcaatt tacgtttaaa
      961 ccagaaatca aaaaagcaga tgattttaat aaacatgcac caaccaaaga agaacaaaat
     1021 aaagcattgg ctttagctga taaacaaaaa gaatacgaag aacctgctac aacaattaac
     1081 gaaaaaatca aacagaagaa acgagaactt gaactcctcc aacaaatcaa acaactagaa
     1141 aaagagaaag aagctcttga aaaacaaaca gagtccgcac tttcgaaaaa agagaaaaaa
     1201 agtttattat ctgatttaga taatttattc cagtagcaac ctaggaggca accagtatgc
     1261 aaccacaaga aacctttaca ataaaggaat tgtcagaact ttttaagatg agtaggcaag
     1321 caatatcgaa acatatccaa aaattagatt cttccatgat agctaaaaat gagcgtggtt
     1381 ataaggttgt attgcgttct ggagtattgc aacttgctcg caacttaggc aaccaaatgt
     1441 tgctagatag gttgcacgag tacgaacaag acaacgataa aaatgttaat ataccaagca
     1501 aaaacgtggt tacaaacaag ttgctagatc agttgcgtga agaaaatgaa tttttacgag
     1561 aacaacttgc cctcagagag caaaataatt ctcatataca aaaactactc gaccagcaac
     1621 aacaactcac cctacaagcc aacaaacaaa tcgaaaaact gcaagaaaaa ctacagttga
     1681 cttatacaga agaagctcca gagaatgact caacgactct ttctgaagca acagaaacaa
     1741 tagacaaaca aattgatact gaaaaaaaat ggtggcaact ttggaaataa ctggagggaa
     1801 cattcatgac aagaaaaaat cttctattcg gttctatttt actaggaatt ctaggattca
     1861 gtccgatgtt attgtcagtg atcaataaag ggaataaaca tggtgaattg aaattgaatc
     1921 caaattatta caacagtcct aacgaagaat tgtttgtaaa agaaacagat aaataataaa
     1981 aaagactgct ctttcgagca gccagttaaa aaataatgag tgtttattct tgaaaaagca
     2041 acgcgcttat gaagcgctac caaaaattta tcatatttta ttaaaaataa caacccagta
     2101 aaaaatgact ggtaaaaatt cacaaagaaa cggaaagaag caaccgcaga aaatcaagct
     2161 taaaaagagc ttttgaccta gaccaatcga aaatcacaaa gtgagcgcct gtatctattg
     2221 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
     2281 tcctaatgca ttctaactat accacattat caaaaatata aattatcttt actaaataaa
     2341 atattgacaa gcctatacct tttgttttat gattttcaca aagaaaacgt tttctatata
     2401 tttttctgta tcaggaggaa attctatgaa aaaatttact ttaggggtag ctttgttaac
     2461 tattggggca gttgttttac cgtcaactac tatagtttat gcttcagaaa attcccaagt
     2521 ggtatcatta aacgaaaaca atgctactga aaatgtagtt attgagtaca ctgaagatgg
     2581 tatggtcgta actaaatatg aggatggtta tagtgcggaa gattttgcaa attcttcaga
     2641 tatcatgaca agagctgcag ttgcaagatg gggcaaaaag gaatatacaa atattgctgt
     2701 aaccactgga gtagctgcta atgctattaa tgctgccttt tgggcaggag ggaaagctag
     2761 cacttctatg tttggtatcc caggttgggc aattggtgct ttgttaacag gtgcaagttg
     2821 gactaaaaaa ggtgcttctc cagggaatgc agttgcaaaa gaatgggaca aaaatggtaa
     2881 tggctggatt ggattttatt accaaagggg atatgatgca gctggaagag ttgtagctac
     2941 tagatattca acgttataag ttaaggagtg atgtttttga tatctaaatc attaaaatca
     3001 ttaaaaacat ttactaaaaa gaatccgcaa atagctacta ttcgtatttt tatatttgct
     3061 atagctgttg ctataattgt taatatgcta agcggagggc aattatacaa atctggttta
     3121 tttttagtgt taatcttagt tgtcttagac ataataaagt ctaaaaaaaa ataaatcgat
     3181 acattttaga agaatagggt tgtttgaata ttacaaagca acaatttcac cttcggtgaa
     3241 ataagcgaaa ctttacaaaa agtggctagc aaatgctagc ctttttttgt attgtggagc
     3301 gtggattttg agggggattc aagggggagc ttgcgctcct ttgacaagcg gttatttgca
     3361 ccaaatacgg aggtatttgg ctagcaaata tagagcttgc caaacccttt tagtgagctt
     3421 tactcattgt acctttttag gtacaatggt attgcgaacg atacgtccgt aggacgtact
     3481 gaaagggtgg gacttatgag cgaacaacat caaaatttgg ctagcgatca ttctaaaaaa
     3541 tatacctatc gatccgagcc aaaacaaatc agttttcgag tgagcgaatc cgaatttgca
     3601 aagctgaagc agtcagctga agctttgcaa atgagtgtgc cggctttcgt gaaagccaag
     3661 gcacaaaacg cgcgtctggt gacaccgaaa gtggcgccag acattgccca ggcaatcgcg
     3721 cgcgatttag ccaaagctgg agggaatatc aatcaaattg ccaagtggtg caatacacac
     3781 caacacgacg ttgctcctga tgatgctcaa cgtttatccg aaaacctaaa aatcatgcaa
     3841 aaggagctac aaaagatatg gcaacagtta aagtaagtcg aaccacttca tgtagccgag
     3901 caatcaatta cgcagaacca cgagcaaccg tcaaaacagg aataaactgt gacattgatt
     3961 atgccaaaag tgaaatgaaa caaatacgca cgctttatgg aaaagacgat cgtgtacaag
     4021 cacacttatt gattcagtcc tttcgaccag gagaaattac cgctgaaaaa gccaatcaac
     4081 taggggcaga gtatgcgaaa aaaatagccc ctaaccacca aatcgcgatt tacacacata
     4141 cagacaaaga ccatattcat aatcacattg tcattaactc cgttaatctt gaaactggga
     4201 aaaaatttca agcccatggg caagcatttt taaacaaatg ttatgaccta aatgacgaga
     4261 tttgtcttga acatgactta acaatcacgg aacgaggaaa gaaacccgaa aaacgaacca
     4321 tgtctgaaat taaattaaaa gaaaaaaatg aaccggtttg gaaagacgaa attcggtttg
     4381 cgattgatca aacaatgaaa aacaaaaaaa ctcgaacgta tacccaattt tgtgattcac
     4441 ttaaaacatt cggcattcat tgcttgaatc gcggaaaaaa ttttacttac gaattggtcc
     4501 acaaaaagaa aaaagtacga tccaacaaac taggaaagga ttacgaaaag gagacgattt
     4561 tacatgagct ggacagacga gaacgaacct acaatcaaca acgaaccaac gagctacgaa
     4621 gagctcgagc tagcattcgc gaaactgcag aagaagtacg aagagaacaa cgaactttta
     4681 cgcctgaaca atcggaaatt agaccgactc gaagaaacca tgaacaacac gttgaacgag
     4741 gaacagacca ccaacaagaa ttatcgctct aaaacgttaa aagacatcga aaacgaaata
     4801 aagagacaac gtgcctttct tgcgccttat acccaagaaa ggatacaaac tacgatggat
     4861 tcacaagtca aaaaactcca ccaagcgcta ttagaccagc gaaaccaaga acaaaaaaag
     4921 gctttaattc aacgatcgat ggatattctt gcccccttat gttgcttagt actaacatta
     4981 ttagcctttt ggttggtatg gggagaataa acaga
//
LOCUS       DAJSAW010000106         4968 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJSAW010000106 DAJSAW010000000
VERSION     DAJSAW010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.108"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..182)
                     /locus_tag="ONT64_002667"
     CDS             complement(<1..182)
                     /locus_tag="ONT64_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2304039.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ONT64_002668"
     CDS             517..951
                     /locus_tag="ONT64_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR2304040.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ONT64_002669"
     CDS             974..1294
                     /locus_tag="ONT64_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304041.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ONT64_002670"
     CDS             1435..1626
                     /locus_tag="ONT64_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304042.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ONT64_002671"
     CDS             complement(1697..1954)
                     /locus_tag="ONT64_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HCR2304043.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ONT64_002672"
     CDS             2112..2375
                     /locus_tag="ONT64_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304044.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ONT64_002673"
     CDS             2399..2587
                     /locus_tag="ONT64_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304045.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ONT64_002674"
     CDS             complement(2670..2855)
                     /locus_tag="ONT64_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304046.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ONT64_002675"
     CDS             3070..3441
                     /locus_tag="ONT64_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR2304047.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ONT64_002676"
     tRNA            complement(3591..3663)
                     /locus_tag="ONT64_002676"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ONT64_002677"
     CDS             complement(3709..4137)
                     /locus_tag="ONT64_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304048.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ONT64_002678"
     CDS             complement(4197..4955)
                     /locus_tag="ONT64_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCR2304049.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1735 a          779 c          975 g         1479 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctccct
//
LOCUS       DAJSAW010000107         4886 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJSAW010000107 DAJSAW010000000
VERSION     DAJSAW010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.111"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(6..284)
                     /locus_tag="ONT64_002679"
     CDS             complement(6..284)
                     /locus_tag="ONT64_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304050.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(244..1158)
                     /locus_tag="ONT64_002680"
     CDS             complement(244..1158)
                     /locus_tag="ONT64_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR2304051.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(1140..1520)
                     /locus_tag="ONT64_002681"
     CDS             complement(1140..1520)
                     /locus_tag="ONT64_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR2304052.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(1970..2257)
                     /locus_tag="ONT64_002682"
     CDS             complement(1970..2257)
                     /locus_tag="ONT64_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR2304053.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(2277..2501)
                     /locus_tag="ONT64_002683"
     CDS             complement(2277..2501)
                     /locus_tag="ONT64_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HCR2304054.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(2956..3126)
                     /locus_tag="ONT64_002684"
     CDS             complement(2956..3126)
                     /locus_tag="ONT64_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304055.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(3141..3674)
                     /locus_tag="ONT64_002685"
     CDS             complement(3141..3674)
                     /locus_tag="ONT64_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCR2304056.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(3695..4639)
                     /locus_tag="ONT64_002686"
     CDS             complement(3695..4639)
                     /locus_tag="ONT64_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HCR2304057.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
BASE COUNT         1270 a          895 c          831 g         1890 t
ORIGIN      
        1 tctatttatt ctccccatac cagccaaaag gctaacagtg tcatgattaa gcaacataag
       61 ggggcaagaa tatccattga tcgctgcata attacttttt tctgttcttt atttcgctgg
      121 tttaatagcg catggtggaa ttttttgact tgtgaatcaa ttgtagtttg tattctttct
      181 ttggtataag gtgcaagaaa agcacgttgt ttctttattt cattttctat gtcttgtaac
      241 gttttagaac gataattctt gttggtagtc tgctcctctt tcaacgtgtt ggtcatggtt
      301 tcttcgagtc ggtctaattt ccggttgttc agtcgtaaga gttcgttgtt ctcttcgtac
      361 ttcttttgca gtttcgcgaa tgctagctcg agctcttcgt agctcgttgg ttcgttgttg
      421 attgtaggct cgttctcgtc tgtccagctc atgtaaaatc gtctcctttt cgtaatcctt
      481 tcctagtttg ttggatcgta cttttttctt tttgtggatc aattcgtaag taaaattttt
      541 cccacgattc aagcaatgaa tgccgaatgt tttaagagaa tcacaaaatt ggttatacgt
      601 tcgagttttt ttgtttttca ttgtttgatc aatcgcaaac cgaatttcgt ctttccaaac
      661 aggttcattt ttttctttta atttgatttc agacatggtt cgtttttcgg gtttcttccc
      721 tcgttcagtg attgttaagt catgttcaag acaaatctcg tcatttaggt cataacattt
      781 gtttaaaaat gcttgaccat gggcttgaaa tttgttccca gtttccagat tgacggagtt
      841 aatgacaatg tggttatgaa tatggtcttt gtctgtatgt gtgtaaatcg cgatttggtg
      901 gtcaggggct atttttttcg catactctgc ccctagttga ttggcttttt cagcggtaat
      961 ttctcctggt cgaaaagact ggatcaataa gtgtgcttgt acacgatcgt cttttccata
     1021 cagcatgcga atttgtttca tttcgctttt ggcataatca atgtcacagt ttatccctgt
     1081 tttgacggtt gctcgtggtt cggcgtaatt gatcgctcgg ctacaagaag tggttcggct
     1141 tactttaact gttgccatat cttttgtagc tccttttgca tgatttttag gttttcggat
     1201 aagcgttggg cgtcgccagg atcaacgtcg tgttggtgtg tgttgcacca cttggcaatt
     1261 tgattgatat tcccgccagc tttggctaaa tcgcgcgcga ttgcctgagc aatgtctggc
     1321 gccactttcg gtgtcaccag acgcgcgttt tgtgccttgg ctttcacgaa agccggcaca
     1381 ctcatttgca aagcttcagc tgactgcttc agctttgcaa attcggattc gctcactcga
     1441 aaactgattt gttttggctc ggatcgatag gtatattttt tagaatgatt gctagccaaa
     1501 tattgatttt gttcgctcat aagtcccacc ctttcagtac gtcctacgga cgtatcgttc
     1561 gcaataccat tgtacctaaa aaggtacaat aagtaaagct cactaaagct cactaaaggg
     1621 cttggcaagc tctatatttg ctagccaaat acctacgtat ttggtgcaaa taaccgcttg
     1681 tcaaaggggc gcaagctccc ccttgaatcc ccctcccccc cactctccac aatacaaaaa
     1741 agactagcaa atgctagcca ctttttgtaa agtttcgctt gtttcacctt cggtgaaatt
     1801 gttgcaaatc aataattagg tagagctaca tttttttcgt tgttcaaaga ttcttatgcc
     1861 atttactatt aataaatcta taaatatgat aatttttcgg ctctataatt tatgggactg
     1921 atgaatcaga attgattctt cagtcccatt ttcattttat tccaaagaat taactccaaa
     1981 taccaataga agcccatcct ctatatactt gagattcctt actaacaatt tcttgtaatt
     2041 ctattaaaga tttaggtaat ccaaatttat gttttaaaat atatcttcca agaaaactac
     2101 ttaatttcat actaacttca ccaacacttt ttccctcact gatttcttta gaataaagaa
     2161 agagcttttc ttttaagtca ggatagtcat ctatagttgc ttcattgtat actttactaa
     2221 ttgcatttaa aagtttttct tctttagtaa aatccattat aataacctcc ctaccactac
     2281 cttctaggtg cccatggacc atgattaacc caaccattaa caataatttt tccaatttct
     2341 cctttagctt gattccaatc tacccaacat ttttctttgt tacaataaag accatttcca
     2401 tagtaagtag ctgcatcaac ttttattcct gtaccgatgc cagctagaca agttcctaat
     2461 attcctagaa taacacaatg ttttaatact ttctttttca tgattgtttt tccttttcgt
     2521 ttttttaaaa ttgcttgtta tattaaataa tcttagtaat tttattatat aacattttat
     2581 tatataacaa tgattaacgt cataaaaagt agttttgaat tcataatcgt ttaaataaaa
     2641 ttataagatc agttatttaa acagctagta gacataatgt gtatactacc aagtaacgcc
     2701 tgtaccaagg gatccaggcg ctgattttgt gaatttttat tggtctaggt taaaagctct
     2761 ttttaaggct tggttttctg cggttgtttc tttctaattc tttgtgaata ttgatcggtc
     2821 attttttaaa tacttgttat ttttgtttgt gtttgttatg ctttttacat atctcatatt
     2881 catttattat tcttttctaa ggttaataat aggataatct ctttcagaaa gactgctcgt
     2941 acgagcagtc ttttcttatt tatcaagttt ttctacaaat acttcctcat tttgactgtt
     3001 gtagtaatta ggattgagtt ttagttcccc ttttttattt cctttgttgg ccactgataa
     3061 taacattggg ctaaagctta atattcctag taggatagaa ccgaatagca agtgtttttt
     3121 tgtcataagt atttccttct tcatttccat agctgccacc attttttcca attctttgtt
     3181 tgttcgtcca ttgtttctgt tgcttcagaa agagtcgttg aactattctc tgaagactct
     3241 tccgtataag tcaactgcag ttgctcctga agtttttcga tttgttggtt ggcttgcagg
     3301 gtgagttgtt gttgctggtc gagtagtttt tgtatatgag aattattttg ctctttgagg
     3361 gcaagttgtt ctcgtaaaaa ttcattttct tcacgcaact gatctagcaa cttgtttgca
     3421 accacgtttt tgtttggtat ataagcattt ttatcgttgt tttgttcatg tttatgcaac
     3481 ttatctagca acctttgatt gtctaagttg cgagcaagtt gcaatactcc agaacgcaat
     3541 acaaccttat aaccacgctc atttttagct atcatggaag aatctaattt ttggatatgt
     3601 ttcgatattg cttgcctact catcttaaaa agatctgaca attcctttat tgtaaaggtt
     3661 tcttgtggtt gcatactggt tgcctcctag gttgctactg gaataaatta tctaaatcag
     3721 ataataaact ttttttctct ttttccgaaa gtgcggactc tgtttgtttt tcaagagctt
     3781 ttttctcttt ttctagttgt ttgatttgtt ggaggagttc aagttctcgt ttcttctgtt
     3841 tgattttttc gttaattgtt gtagcgggtt cttcgtattc tttttgttta tcagctaaag
     3901 ccaatgcttt attttggtct tctttggttg gtgcatgttt gttaaagtcg tctgcttttt
     3961 tgatctcagg tttgaacgta aattgatagc caataacagg ttttccccgt ccttttccgt
     4021 atttcttttt gattgttagt cctttgaaga ttgcagttag ttcttgacgt ataggtttta
     4081 taactcgttt atctatatca ggttgcttat aacttttagg aatatctaat aattcaagaa
     4141 agtcttcttt ggagaaatat gcgaaacctt tggttctgta ttgctttaac agacgaaaca
     4201 tagtctttgc atagccactt tgtagctcgt tgaattgttg taaagaatag cgtacccact
     4261 cgtctaaatt atttaacaaa ggcagtgctt tttcgtaaat tcgtatatct acgtaaggat
     4321 tttctacgtc cccgttgatt ttaaattctg taaacattac aaacatatcg taagaaagcc
     4381 cagactcact tctacgacca aagcgtaaag ataagatttt ttcataagtt gcttttaaat
     4441 cttctacaaa tcgtacattt gcagttgctt tgtagttact taaatctttt agttggtcaa
     4501 acgtaaaacg aactgtctta tccccttttt cacgcattct agacacgatg gaaaaaaaca
     4561 aattcatttc aaccggtgta aattttctta aaggaattgt atttaattca tggtggtatt
     4621 ttacaatctc attagccata taatcacctc aatccaatta tgaatgacat tttttaaatg
     4681 tcaagacatt catatttgac gttcaaacag acaaaatgtc cttcataaac agacaaaatg
     4741 tccttcataa acagacaaaa tgtccttcat aaacagacaa aatgtccttc ataacattct
     4801 gtatccattg gtagagtaag gacacagctt acggtaaaga ggtttaaaaa gaggtttaaa
     4861 aagagatata aaaagagggg ggctaa
//
LOCUS       DAJSAW010000108         4843 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJSAW010000108 DAJSAW010000000
VERSION     DAJSAW010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.112"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..258)
                     /locus_tag="ONT64_002687"
     CDS             complement(<1..258)
                     /locus_tag="ONT64_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304058.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="ONT64_002688"
     CDS             complement(274..924)
                     /locus_tag="ONT64_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304059.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="ONT64_002689"
     CDS             complement(935..2107)
                     /locus_tag="ONT64_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCR2304060.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="ONT64_002690"
     CDS             complement(2126..2998)
                     /locus_tag="ONT64_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2304061.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="ONT64_002691"
     CDS             complement(3020..3940)
                     /locus_tag="ONT64_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304062.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="ONT64_002692"
     CDS             complement(3940..4209)
                     /locus_tag="ONT64_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304063.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="ONT64_002693"
     CDS             complement(4206..4586)
                     /locus_tag="ONT64_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR2304064.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
BASE COUNT         1270 a         1060 c          794 g         1719 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tat
//
LOCUS       DAJSAW010000109         4813 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJSAW010000109 DAJSAW010000000
VERSION     DAJSAW010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.113"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            68..1114
                     /locus_tag="ONT64_002694"
     CDS             68..1114
                     /locus_tag="ONT64_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR2304065.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            1131..2354
                     /locus_tag="ONT64_002695"
     CDS             1131..2354
                     /locus_tag="ONT64_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR2304066.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            2443..3330
                     /locus_tag="ONT64_002696"
     CDS             2443..3330
                     /locus_tag="ONT64_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR2304067.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            3416..3646
                     /locus_tag="ONT64_002697"
     CDS             3416..3646
                     /locus_tag="ONT64_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HCR2304068.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(3703..4668)
                     /locus_tag="ONT64_002698"
     CDS             complement(3703..4668)
                     /locus_tag="ONT64_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR2304069.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         1631 a          795 c          976 g         1411 t
ORIGIN      
        1 atagggctta agtttgagtg acagtttgta ggctatgtta tactatataa gtgaaggggg
       61 acgtaaggtg aaggtattat tatattttga aagtgaaaag atgcttgcaa aatctggaat
      121 cgggcgtgcg cttgatcatc aaaaacgtgc tctgacggaa gtcggtatct cttatacact
      181 agatgaaaaa gaagattatg acatcctgca catcaataca tacggaatca atagtcataa
      241 tatggtcaat aaagccagaa gagaaggaaa aaaagttgtc tatcatgcgc attctacaga
      301 ggaagatttc cgaaattcct ttatcggatc gaaccagctt tctcctatcg taaaaaaata
      361 tctggtagga ttgtaccaaa aagcggacta tctgatcacg ccaacacctt attcaaaaca
      421 gttgttggaa agctatggta tccgtgtgcc gattcaagct atttccaatg ggatcgactt
      481 agagaaatat caccctgatc caatgaaaga acaaaagttc agagaatatt tcaaactgtc
      541 tccggatcaa aaagtaatca tttgtgtcgg tttgtttttc gaacgtaaag gaatcattga
      601 tttcgtagaa attgctaaga agatgcccga atacacattt atttggtttg gtcatgttcc
      661 catgtactcg attcctcgga acatccggaa aattgtaaaa gaagatcatc ctgaaaatgt
      721 cctgtttcct ggttacattc gtggagagat catagaaggt gcgtattcag gtgcagatct
      781 attctttttc ccttcttatg aagagacaga agggatcgtt gttttagaag ctttagcaag
      841 caaacaaaac gtacttgttc gagatattcc agtgtataat ggctggttag aagatagcaa
      901 gaactgctat atggggaaga caaacgaaga atttatccga ctgatccaac agattaccaa
      961 tcaagaacta ccgagtacga cacaagccgg ttatcaaaca gcaaaagagc gaagtatcaa
     1021 taaaatcggt gaagaactta aaaatgtata cgaaagtgta ctgaatgaaa aagtctctag
     1081 caaattaaaa aaagtaaatc aggtcaagga ctagtttgga ggcgaaaaac atgaaaatcg
     1141 gcttttttac cgatacctac tttccgcaag tcagcggagt tgctacatcg atcaaaacat
     1201 taaaacaaga attagaaaga catgggcatg aagtctatat ttttacaacg accgatccta
     1261 atgcggataa gttggaaaaa gatgtgattc gaatgccaag tgtaccattt atttctttca
     1321 aggatcgaag agttgttgtt agaggcatgt ggtatgctta tttggttgcc aaagaactag
     1381 aactagatct tattcatacc catacagaat tcggagcagg gatacttggg aagatggtag
     1441 ctaagaagtt gaaaatacct ctgatccata cttaccatac gatgtatgaa gactaccttc
     1501 attatatcgg taaaggaaaa gtgatcaggc aatcccatgt aaaatacttg tctagattat
     1561 ttgcaaatca tacgacaggt gtagtttgtc caagtgaacg tgttattgat acattacgca
     1621 gctatggtgt aatagcaccg cttcgcgtga ttcctactgg tatcgacatt gaaaaattca
     1681 aacgttctga tatcactcag caggatatca gcaaattaag ggagtcacta ggtcttcagg
     1741 aaaaccattt gatgattctt tctttaagca ggatttctta tgagaaaaat atccaagcct
     1801 tgattaatgg gtttcctcag gtcatctccg cttatcctaa cgccagatta gttatcgtag
     1861 gaaaaggacc ttatgtagaa gagttagagg aattgatctc agaactgcag ttagaagaat
     1921 ttgtgcagtt cacaggagaa gtggacaaca atgatgtagc actttattat aaagcggcag
     1981 attattttgt cagtgcttct acttcagaaa cacaagggct tacttataca gaagcgatgg
     2041 cagccggaac accttgtgtc gtggaaggaa atgcttattt gaaccgattg ttcgatcatc
     2101 cttcattagg gaaaactttt gaaaccgacg aagactttgc accagcactt attgattata
     2161 tttccagtga agttcctaaa gatccaaaag ttttagaagc taaactttat gatatttcat
     2221 ctacacattt cggcgaagct atgatcgaat tttatcaaga catggttgtt tatcatgaag
     2281 aagagatgcg aagaaaagaa gaagaagact cgattgaacg aatcaaaata aaattaaatt
     2341 catttaaaag ataaaattca aacatttttt atgaaaaaat ccttgtttct agcgtatact
     2401 agcatagaag caaggatttt tgatttttag gaggtcgaaa aaatgaaact tggttttatc
     2461 ggaacaggcg tcatgggaag tgctgtcgca cgccatctac tagaagcagg acacgaagta
     2521 gctgtctata atcggacaaa agcaaaagct gatcctttag taacagaagg agctatctgg
     2581 gcagatacac caaaagcggt agcagaacaa agtaatattc tctttacaat ggtaggttac
     2641 cccaaagatg tagaagaaat ctactatgga cagtcgggga ttttttctgc agatatctca
     2701 ggacatattt tagttgattt gactacaagt acaccaagcc tggcagaaaa aattgcaaaa
     2761 acagcaaaag aaaaaggcgc agatgcgtta gatgcaccgg tatccggtgg tgacttagga
     2821 gcaaagaatg gtacactgac gatcatggtc ggtggagaag aagccgttta tgatcaagta
     2881 ctgcctttat tcaaagaatt tggtacgaca tttacattgc atggttctgc tgggaaaggt
     2941 cagcatacaa aaatggctaa tcagatcatg attgcaggta cgatgacagg aatgacggaa
     3001 atgcttgtct atgcacaaaa aaacggactt gatttaaaga aagtgattga gacgctttca
     3061 ggtggaagtg cagctaactg gtctttaagc aattattctc ctcgtatttt aaaagaagat
     3121 tacacacctg gtttttttgt caaacatttt atcaaagatt taaagattgc attagaagaa
     3181 gcagaaaaaa tggatcttgt attgccagca actacccaag cattgaaact atatgaagag
     3241 ttggcagata aaggttttga gaatgacgga acccaagctt tgatcaaact ttggtggcct
     3301 gaaggaaaaa taccagaaaa aaaatcataa aaaacgagtt tttttagaga aaatcaatgc
     3361 aagtaggcta tgcttttgat acaatataac agaaataaga aaaggaggac ctccgatgac
     3421 acattcacaa ttagtcgcaa tcataaaacg actggaggct atggtcgagt cagcagataa
     3481 tgaactgcaa gttcgccgtt ttgaaaaaga aggcgtagag aagtgtacag taacttatga
     3541 taaagcgacc gatacatttg aattaactga aacagatact catcagcaat acgaatttga
     3601 taatatcgat atcgtagcga tggaaatcta tgacttaatc caataaattt taggataatt
     3661 gacatttgaa gttgaagatg cctaaaagaa gccgagaagc aactaattta gttgcttttc
     3721 agcttctttt ttgtttaaca gttcaatttc aaactgttct ttttctaatt ttttgcggta
     3781 actgatcaag atttgtacag gatcgatttc ttcttttgac aattgacgtt tcaaagataa
     3841 atgagcttct ttcttttcat aatagaagtc aggagaaacg tcaccagagg taatcattgt
     3901 ttctgcaagt tgaccataac aaacgaggta gtgctttttc tgactctcat ctgctgctaa
     3961 tggcatgctg aaggtgagcg atggtgttct taaaaactgc ttttgttcct ggatccgtct
     4021 aataaaataa gtaactaatt cgatatcttt ttgaagcata ccggcttttt cgaaattcaa
     4081 ctgatagatg aaatgttgtt gtcttttttt caagtaagtc agtagctttt tatttcggcc
     4141 ttgaaacagc ccttggagtt cctttttttt ctgttcaaaa gtcatttcct gtaggtcagg
     4201 aagctggact cgcaaagcta acaaactaat ttcagacaac caaggcattt gataagtttc
     4261 ttccattata cgcaaaatag acggcatttt tttatagaga cgaaaaggcc cgtatgttcg
     4321 agccgtagga gtatctgtta atacaaatcc cttattggta atatgtacat aattataatt
     4381 actgaaataa ttcatttgtc ggttataaaa aggacggtaa tgttggatca attgacactc
     4441 gagcaataaa gcatctaatt ccgtgtctac tacgacgaaa tcaaaatctg ttatatgatg
     4501 aatcatacgt aatacttttt ttgaatgctg tttattctga tgaaaatagc tgcttactct
     4561 atttttgagc cgttttgctt taccaacata gatgacctta ccatctcggt ctttcataag
     4621 atagacaccc ggcagcatgg ggagagtttt cattttttct tttaacatag gatctccttt
     4681 tacgctttga ataaaaagac tataacaaat tttgaaataa tggacaagtt ttagggaatt
     4741 ctatagttct gttcacagaa aatactttga taatcaaaat atgtgagata gatgattttt
     4801 ctaataaaag aaa
//
LOCUS       DAJSAW010000110         4796 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJSAW010000110 DAJSAW010000000
VERSION     DAJSAW010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.114"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(57..173)
                     /locus_tag="ONT64_002699"
     CDS             complement(57..173)
                     /locus_tag="ONT64_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCR2304070.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(418..495)
                     /locus_tag="ONT64_002700"
                     /pseudo
     CDS             complement(418..495)
                     /locus_tag="ONT64_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(649..1284)
                     /locus_tag="ONT64_002701"
     CDS             complement(649..1284)
                     /locus_tag="ONT64_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2304071.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            1797..2939
                     /locus_tag="ONT64_002702"
     CDS             1797..2939
                     /locus_tag="ONT64_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2304072.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYVCDNPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYEY
                     GYYGYKEHYAYGEIKVMVSDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPTLSEKYKNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDNLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            2932..3138
                     /locus_tag="ONT64_002703"
     CDS             2932..3138
                     /locus_tag="ONT64_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR2304073.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            3217..4410
                     /locus_tag="ONT64_002704"
     CDS             3217..4410
                     /locus_tag="ONT64_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2304074.1"
                     /translation="MKEKRRDSKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDRLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFDFKLSEVLENDTKEKVALTEEQEQALLSFIKTDNVYHKYYDDVLI
                     LLKTGLRISELCGLTVADIDFQNEVVIIDHQLLKSKEQGYYIETPKTKSGTRQVPLSR
                     ETIQAFQRVMKKRPKAEPFVIDGRGNFLFINHKGKPKVAIDYTMLFVRMVKKYNKNHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
BASE COUNT         1624 a          929 c          918 g         1325 t
ORIGIN      
        1 gcatcaacat gagctaaaac tccaatatta ataattttca tgtgattttc ctccattcaa
       61 aagcccaaaa gggcataaaa atcccagtga taaatactct tatcactggg atttttatgc
      121 ataaccatag gcatacaaag catacagata ttctccggat actttagaat cacatgataa
      181 aggtattctt aaactgggta caaaaaacta agccctccta aaaaaggaca tccaattatt
      241 tgttcccact atcaaattga cagtttattt aagaatacct tgccgcatat ttattaactc
      301 cttttaaata gatacttaaa taatagcacg taagagcata tttgtaaagg aatctccaat
      361 tttttatcaa agagagtacg tgattacaaa atagctgtaa taatgtacca atatttgtta
      421 ttctataatc ttccaattac tcccgttctt ttcaagtacc aaatcaaatt gagatacctg
      481 cgttgccttt gcctgattta ccacaaatca gattttatcc ctttcttcct cttgattgcc
      541 cacaacagaa aagaaaaagc cctgtcaaga gctttgccac cattggtggt gctttgcact
      601 cttgatcggg ctttttgttt gctgtatctt ctcgcaagag gaaaaaagtt actcgtcctc
      661 atcatcaaaa ttgttattcc ttaatacttc tcgtaacagt tccatatctt cttttgagtt
      721 cgggtttatc attttcacga cttgataagg tgtcttatac ttatgtccct ctatgctttc
      781 cccaaacata tccatactgt ataaatcatc atagcggtct aataattttt gaaacttcaa
      841 atgctggata aattctttga ttgggtaaag gtcggacgct tcaatgcttt tcccgttgat
      901 atagtctgta atatattccc gtaacttttc taactcatat tccagccatt cttcctcgct
      961 gtcaaagaag ttatcatagt gtccatgttt cagttctgca tttagacgtt cttcggctct
     1021 taaaaactga cagacttcct tttcagattg attgacatac ttccattctc ccgacaacaa
     1081 ttcattgggc gtaacgtcca gcacttccat tatcttttcc agcgtttcat aggcaggata
     1141 gttcaaccct ctttcaatct tggaaaggct ctgcatattg ataccgattt tgtccgcaag
     1201 ttcctgttgt ttcattcccc taaattttct tatggtctgt atattatttc ctaaatggct
     1261 tattttcatt gcataaccct ccatatctcg ttatatcttc gctattataa gtctatcatg
     1321 cttaatatga tttgtaaagg caaaaaaaga tattgacaaa taagcatata agaattacta
     1381 tgttcttgta atcgaataag cacaatagaa tgaaatataa aaacattcaa aaagaaatac
     1441 ctatctgatt aaatccgtag atatgaatat cggttgaaat ggaacttgtt agaaaaggca
     1501 aaacagaact tgctgacaaa gtatttctga cacggtggcg gaagcctctc aggcttaccg
     1561 ccatgacacc ttgtgtcaag gggaagaaat gctttgtcgg ctggcgagcc tgcgagcctt
     1621 gtctgtgccc ccgttatcta acaggggggc actatttcac aaaagacaag tcaaaaacca
     1681 tacaacccca aagctgtacg gagtaatgag gagttttaag aagatagtac acttcacact
     1741 tttttgaaag gcggtttaca ggtatttcaa cgctattgtg ggattggagg aatacaatga
     1801 acaacttaga atttgcactt gaaatgaaga acaaacgtct tgccagcggt ctttcacaag
     1861 gagaacttgc aagtcttacc catgtatcaa gatatagtat caaccgcttt gagaacggaa
     1921 aagccaacgc cagcaaggaa acacaaaata ttatcctgcg ttgtctgaat tactatgtct
     1981 gtgataaccc tttttatctg ctggtggatt atctgtctgt ccgtttccca acgactgacg
     2041 cattggaagt tatccgaaag gtgcttggca tgaaagcaga ttattttatc cattatgaat
     2101 atgggtatta tggctataag gagcattatg cctacggaga aatcaaggtt atggtttctg
     2161 atgatgaaca catgggcgta tttttggaat taaaaggggc aggctcacgc aacatggaat
     2221 acgtcttaca ggcacagaac agggattggt attcattctt aaaccgctgt cttgactgtg
     2281 gcggtgttat ccgacgtttt gacttggcaa tcaacgatat gtgcggtctg ctggatattc
     2341 ccaccttgtc tgaaaaatac aaaaatggcg gtgcggaatg tcgctgtaaa aactatgaaa
     2401 atgtgcaggg tggaaaacta agcggaaaaa accgtaattt agcaagcact ctttatatcg
     2461 gctcaaaggc aagcacaaaa tatttctgcc tgtatgaaaa acaaaaggaa caggcaacta
     2521 aaaagaaaca tacggatatt atcaaccgtt ttgaaattcg tctgcgtgac aaaaaggcag
     2581 tacaggcagt tgaagaactg ttattgacgt ataaccctca tgggctggtg ttttacctca
     2641 ttaccgattt tgtggaattt cccgactatc cattgtggga gatattcatt tcccatgata
     2701 atttaccttt tgaaatgaac cctgtacctg tcaatatgga acgcactctg caatggctgg
     2761 aaagacaggt catgccgtcc attgtgatga tagaagaaat cgacaggctg acaggttcaa
     2821 actatatgaa aatgattgat gaatgtaccc acctttccga aaaacaggaa atgcttgtgg
     2881 agcagatgtg tacggacata gcagacgtaa tcgaaagtga gggggtgttt tatgagtaat
     2941 gcacaggata ttcccgtatg ggaaaaatac acccttacca ttgaagaagc gtcaaagtat
     3001 ttccgtatcg gagaaaacaa gttaagacgc ttagcagagg aaaacaagga cgctggctgg
     3061 ctcattatga atggcaaccg catacagatt aaacgccgac agtttgaaaa ggtcattgat
     3121 aaattggacg caatctaatg caaatgagcc ttgtatgtgt tatgatgaac acaagtcata
     3181 tcaaggctct ttccaacaag gaaaggagca gacaccatga aagaaaaaag acgggatagc
     3241 aaaggacgta tcctgcatac tggagagagc caacgaacag acggaaaata cttatataaa
     3301 tatgtggacg catttggaaa cacaaaatat gtgtatgctt ggagattgac acccacagac
     3361 ccgacaccaa aaggaaaacg ggaaaaaccc tcacttcgtg aactggaaca gcagataaga
     3421 cgggatattg aggacggtat cgacagcaca ggcaagaaaa tgacgctttg ccaactttat
     3481 gccaaacaga acgcacagag ggcaaacgtg aagaaaagca cacagaaaca acgggaacag
     3541 ctcatgcgat tactaaaaga ggacaggtta ggtgctagga gcattgatac gataaaaccc
     3601 tctgacgcta aggaatgggc gttacgcatg aaagataaag gcttttccta taacaccatt
     3661 aacaaccata aacgctcgtt aaaagcgtcg ttctatatcg ccatacaaga cgattgtgta
     3721 aggaaaaacc cttttgattt caagttaagt gaagtcctag aaaatgatac caaagagaaa
     3781 gtcgcattga cagaagaaca ggaacaagcc ctactctcat tcatcaagac ggacaatgtg
     3841 tatcacaagt attatgatga tgtgctgata ctgttaaaga caggacttcg tatctcggaa
     3901 ctgtgcggac tgacagtagc cgatattgat ttccaaaatg aagttgtgat tatcgaccac
     3961 cagttattaa aaagcaagga acagggttat tacattgaaa cgcctaagac aaaaagcgga
     4021 acaaggcaag tgccattaag cagagaaacg atacaggcat ttcaacgggt tatgaagaaa
     4081 cgcccaaagg cagaaccatt tgtgatagac ggacggggca actttctatt tatcaatcat
     4141 aaaggcaagc ccaaagttgc cattgattac actatgcttt ttgtccgtat ggtaaaaaaa
     4201 tacaataaga accataagga caacccattg ccacatatca caccgcatac actacgccat
     4261 acgttctgca caaggctggc aagcaagaac atgaacccga aagatttaca gtatatcatg
     4321 ggacattcaa atatcagtat cacaatgaac tggtatgccc atgcgtccat agataccgca
     4381 aaatcagagg ttcagcgtct aatcgcataa gaagtattta ccacgatttt aaccacgatt
     4441 gacagggaaa atataagaag atagacctag atatgtgagg tttaccacaa aagcaaaatg
     4501 cccgtagagc cgataaaata aggctttacg gacatttaag aagatataaa aagatagtca
     4561 aaaagacata tataatttat acctaattta agtaagtaca aacatcattt taagaatagg
     4621 aaaggacatt tatatactat tctctgtatt tttgatttat aaataaatat gaattgaatc
     4681 caaatcagct atattactat tttataataa ccaactataa aaaacgaagt taggtgaaaa
     4741 actaacttcg atattcataa aagactctat tagttcaact tagcaaaaga tttaaa
//
LOCUS       DAJSAW010000111         4792 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJSAW010000111 DAJSAW010000000
VERSION     DAJSAW010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.115"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..867
                     /locus_tag="ONT64_002705"
     CDS             <1..867
                     /locus_tag="ONT64_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR2304075.1"
                     /translation="VGDKKVQFTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFG
                     RRSADGLQRERFVLFNQFKIDGKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNEL
                     ESSYSKTMFRLLKQYRTKGIAYFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAI
                     FKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYS
                     EKDPTSLQEKIKEQKRKLELLQQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKK
                     GLLDNLQNLFKE"
     gene            877..1374
                     /locus_tag="ONT64_002706"
     CDS             877..1374
                     /locus_tag="ONT64_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2304076.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            1750..2031
                     /locus_tag="ONT64_002707"
     CDS             1750..2031
                     /locus_tag="ONT64_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304077.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            2033..2449
                     /locus_tag="ONT64_002708"
     CDS             2033..2449
                     /locus_tag="ONT64_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304078.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            3101..3526
                     /locus_tag="ONT64_002709"
     CDS             3101..3526
                     /locus_tag="ONT64_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304079.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            3556..3720
                     /locus_tag="ONT64_002710"
     CDS             3556..3720
                     /locus_tag="ONT64_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304080.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            4678..>4792
                     /locus_tag="ONT64_002711"
     CDS             4678..>4792
                     /locus_tag="ONT64_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR2304081.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDV"
BASE COUNT         1758 a          816 c          788 g         1430 t
ORIGIN      
        1 gtaggcgata aaaaagtaca atttacattc gaacaattaa aagatttaag caattataaa
       61 gcaactgcaa atgttcgatt catagatgat ttagaaacaa cgtatgacaa attaatggat
      121 ttacgttttg gtagaagaag tgctgacggt ttgcaacgtg aacgatttgt tttatttaat
      181 caatttaaaa tcgatggaaa agctgatatt ccttttgctg aaatacaagt ccatgagaaa
      241 gccttaccct tacttaataa tcttgaagaa tgggttcgat attctctcca acaattcaat
      301 gaattagaaa gcagttattc caaaaccatg tttagattat taaaacaata cagaaccaaa
      361 ggaattgcct atttttcaaa agaagacttt catgaattac ttgatatccc taaaagttat
      421 agagaaagtg atataaataa aaaaattata aagcctattc gccaagaact aaccgcaatc
      481 ttcaaaggtc tgacaatcaa aaagaaatac ggaaaaggaa gaggaaaacc tgtcattggc
      541 tatcaattca cgtttaaacc tgaaataaaa aaagcagacg actttaataa acacgcacca
      601 acaaaagaag aacaaaataa agctttagaa ctagttaatc aacaaattta ctctgaaaaa
      661 gaccccactt cattacaaga aaaaatcaaa gaacagaaac gaaaattaga acttttacaa
      721 caattagaag agctagaaag aaaacaaaat aatctgaata aagaaaataa ggagattaac
      781 gaacaaatac cagataacaa ttccgatatt cccccagaat taaaaaaagg cttactagat
      841 aatcttcaaa acttatttaa agaataaggg gtttttatga cagataaaac tatcaaagaa
      901 ttagcagata ttctaggtgt ttcaaaagat aaagtgaagt atcaactgag gaaattacct
      961 agtaatttta cctacaaaaa aggaaatatt atctacctga aaaaagaagc agtaacaaca
     1021 attacaaagg ttttatcagg taatgagccg ggtaacttac ctagtgaatt acctgattca
     1081 ttacctaccg attacctact caaggagatt gaaactaaga ataatcatat tgaagaaaaa
     1141 gataggcaaa ttgaaaaact tcaactagct ttagaaacac aacaaaaatt actcgaccag
     1201 caacaacaac tcaccctgca agccaaccaa caaatcgaga aactgcagga acaactacag
     1261 ttgacttata cggaagaatc ttcggaaaat aaatcaacga ctctttctga agaaacagaa
     1321 tcgatagaaa aacaatcaaa gacggagaaa aaatggtggc aattctggca ataaaaccag
     1381 caatttaaat taataaaaat tgctggtttt attcttcttt ttgcttttgc gagcagagca
     1441 acatgggccc atgttgcgat attttggtgg cgccccaatc cccgtttttt cgttgaatct
     1501 tactcggact gcactcgttt tacagctgat ttttctgaat tttgaaagag cataaggaaa
     1561 cgtggtatca ctttcttacg aaaggtgcta gataagctgt gcctatcacg tttctcagaa
     1621 aaaaatttgc tatcactttt ttgccattgt ggctaggcta tgtatagtgt tttggcgaaa
     1681 aatagctata caaatactat atttgtatga tatacataaa ttatatactg atacgaaagg
     1741 aaaatttcca tggacatttt agttcgaaat atcaatccca aaattattac aagaatagat
     1801 aaacgttgtc aggaactaac aaccaaaacg ggcaaaaaat ggagtcgaaa tcaataccta
     1861 aaaattttaa tcgaaaacga ttttgaccac gaacttatga attacaaaaa agatcaattc
     1921 gatttattat tcgaaaaatt tattgccatt caagcagaaa atacgaacat actaaatgac
     1981 tatgttcaca caaacaacca actcattgaa acacttatcg gaggaatata acatgagaaa
     2041 aacttctgtt caaaaacacg taacccttta tcccagtcac gttgacgttt tagattccat
     2101 catcgaaaaa aatccagacg tcaacaatta ttcagaagcc attcgctttt tatgtatgag
     2161 ttatgaacct gctaccaata caaccacaac cagtaaccaa gcaaaactca atgccatggg
     2221 aaaagaaatt tctatcatga cagaaatcat ggcagaattt gctgatttat atctaaaaga
     2281 tactggtgtt cttatcggta caaattctaa agtatatcaa gaagtgaaag aaaaaattga
     2341 aaatcaactc aatccttccc gacgaaatca acccaaaaat cgtcaaaaag aaaccaatca
     2401 aacctttact tctggaaatt ttgacccaac aaactttcta aaaagataaa aaaagaaacc
     2461 gaaaaataaa aaaattcctc gatttctttt tagggatcgg tcttccagca agatttgcaa
     2521 gttggtacca acttgcgaaa aaaaaaagaa aaatccgctt ctgcgaattc ttcttttttc
     2581 attttggggc gctgccctaa tccctaagtc ccggttattc aatagcatgg aaactcaaag
     2641 aataagacgt tccctggaat aaaaaaaagg ctgctcgttc gagcagtcca tacataggaa
     2701 aatgaaaaaa cgacgtccct tactgagaaa cgctattaaa cttttaacaa atcctctaaa
     2761 aaataacaag tctttaaaaa gtgaccggta aaaatgcaca aagaactaaa aaagagaaat
     2821 tgcatcaaat caagcattag aagctagtaa ctagctagac caattgaaaa tcacaaagtg
     2881 agcgcctgta tctattgcta caggcgctac ttggcatagt gtacattatg tcaacaagaa
     2941 tttaatataa catatttttc tagtttgtta taacaatgcc actctaacaa aaatgtaagc
     3001 attatcctta ttaaaagctc cttgacaagg actatacctt ttattttatg atttttacat
     3061 agaaaacgtt ttctctattt tttgtttaga ggtgattttt atgatttgca agaacaagaa
     3121 aaaaattatt gctattattg ctagtttctc tttgattttt agtactgcat gtactgtctt
     3181 attaacaaat aatgctaatg ttgaagctgc aagctcttct tacaaacata atcatgctgg
     3241 ttatagatgt tacaaaatga gttattatgt aagtccttct aaatgcaaac aattggctcg
     3301 ccaaactaat aaacttaata ccgtctcagc tattgcaaac tttattggac tatctggatt
     3361 aacaccgggg attatatcat tagttttcgg atcagcagtt agtgctaata atgtatttgt
     3421 tacagccgct agaagaggaa aaggcgttca attaaattat attgcccatt ttagtaatac
     3481 cactacctat aattatagtt ctaaccaaaa ttatgttata aaataattaa aactttaaac
     3541 taggaggaat gctttatgaa attaacaaaa attgctgtaa ttttagctgt tattgctggc
     3601 attattttat attttgtatt gcctgctaat caattcttcc tttactttgg aatacctgcg
     3661 attttaatag taaatggcgt aagaatcttt gaacaacgaa aaaaatgtag ctctacctaa
     3721 tcgttacaaa acaacaattt caccttcggt gaaataagcg aaactttaca aaaagtggct
     3781 agcaaatgct agcctttttt gtattgtaga gcgtgaattt tgagggggat tcaaggggga
     3841 gcttgcgccc ctttgacaag cggttatttg caccaaatac ctccgtattt ggctagcaat
     3901 ccttctaaaa aatacaccta tcgatcagag tcaaaacaaa tcaattttcg agtgagcgaa
     3961 tccgaatttg caaagctgaa gctttgcaaa tgagtgtgcc ggcttccgta aaaatacata
     4021 tggctataat ttgcgtttta aggcgtttta aacgttctgg tatataatta taccaaaaca
     4081 actttaaaac gccttagatc taattttaga ggtcttatag aacattttag ggttgttagg
     4141 gcttgccctg accgcctgta aggcgctttg agaacaagat aagggtttta tactaagact
     4201 gtcttaaaac ggcttaaaca aacgctcaga acttttcaaa ggagttcttt atgaactgtt
     4261 tttaagcaaa ggagccttta ggcgactgcg cagtataatg tgagagcgta gcgatcacac
     4321 atttttagtg accgtaggga gcgagtttcc cagtttagaa aaattcgcga acttgtttga
     4381 ggaaaaaagc aggagttttt taggcgggaa attttgcctc ctcttttata atctcttttt
     4441 aaacctcttt ttaaacctct ttaccgtcgg ctgtatgctt actctcccaa ggtgtgcaga
     4501 atcttttggg ggtctttttg tctgttttta ggggtctttt tgtctgtttt taggggtctt
     4561 tttgtctgtt tttgggggtc tttttgtctg tttttagggg tctttaatta ggggcttgac
     4621 ccctattaga aagctcttta taattttgtt aagtagttaa tcttgaagga ggattttatg
     4681 gcaaatgaaa ttgtgaaata ccaccatgaa ttaaatacga ttcctttacg caaatttacg
     4741 tcagtagaaa tgaatttatt cttctctata gtatctagaa tgcgtgacgt ag
//
LOCUS       DAJSAW010000112         4775 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJSAW010000112 DAJSAW010000000
VERSION     DAJSAW010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.116"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(207..1169)
                     /locus_tag="ONT64_002712"
     CDS             complement(207..1169)
                     /locus_tag="ONT64_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2304082.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="ONT64_002713"
     CDS             complement(1171..2610)
                     /locus_tag="ONT64_002713"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR2304083.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="ONT64_002714"
     CDS             2795..4741
                     /locus_tag="ONT64_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HCR2304084.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1518 a          818 c          788 g         1651 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tcttt
//
LOCUS       DAJSAW010000113         4710 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJSAW010000113 DAJSAW010000000
VERSION     DAJSAW010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.117"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            106..357
                     /locus_tag="ONT64_002715"
                     /pseudo
     CDS             106..357
                     /locus_tag="ONT64_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="ONT64_002716"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="ONT64_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCR2304085.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="ONT64_002717"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="ONT64_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="ONT64_002718"
     CDS             1786..1869
                     /locus_tag="ONT64_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HCR2304086.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="ONT64_002719"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="ONT64_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCR2304087.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="ONT64_002720"
     CDS             complement(2994..3857)
                     /locus_tag="ONT64_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HCR2304088.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="ONT64_002721"
     CDS             complement(3859..4131)
                     /locus_tag="ONT64_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR2304089.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4357)
                     /locus_tag="ONT64_002722"
     CDS             complement(4148..4357)
                     /locus_tag="ONT64_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HCR2304090.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4710)
                     /locus_tag="ONT64_002723"
     CDS             complement(4455..>4710)
                     /locus_tag="ONT64_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HCR2304091.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1445 a          796 c          865 g         1604 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc
//
LOCUS       DAJSAW010000114         4634 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJSAW010000114 DAJSAW010000000
VERSION     DAJSAW010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.118"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            174..518
                     /locus_tag="ONT64_002724"
     CDS             174..518
                     /locus_tag="ONT64_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR2304092.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="ONT64_002725"
     CDS             519..938
                     /locus_tag="ONT64_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR2304093.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="ONT64_002726"
     CDS             complement(1030..1380)
                     /locus_tag="ONT64_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304094.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="ONT64_002727"
     CDS             complement(1346..2566)
                     /locus_tag="ONT64_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HCR2304095.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2932..3498
                     /locus_tag="ONT64_002728"
     CDS             2932..3498
                     /locus_tag="ONT64_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2304096.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3643..3730
                     /locus_tag="ONT64_002729"
     tRNA            3643..3730
                     /locus_tag="ONT64_002729"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3675..3677,aa:Ser,seq:cga)
     gene            4064..>4634
                     /locus_tag="ONT64_002730"
     CDS             4064..>4634
                     /locus_tag="ONT64_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCR2304097.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1684 a          778 c          891 g         1281 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa actaaaaatg
     2761 taagggaacc cattgaattt aaccaaattt ttttaatttt gcaaaaaaaa gttaaagaag
     2821 actgaaaaaa aatttaaaat ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa
     2881 tagggtacaa taaaacaaca tatactgcag taaagaagtg ggggagtttt aatgagaaga
     2941 aaagtctata ccaaggatca aattctaaaa gctgcttatg atgttgttgc aaaggaaggg
     3001 tttaaaggat ttactgcgag aaatattgct aaaaagatgg ggatttcgac acagccgatt
     3061 tatctggaat tcaaaaacat ggaagattta aaaaatacgt tgctggattc tatctttgaa
     3121 caactaaaag aagatgtttt ccctattgaa cgtactggtg ataaaatcat tgatctcggt
     3181 ttgaactata tctactttgc aaaaaagcat catagtcttt acatggcatt gtacattgat
     3241 gaacatgggg acggaaagaa aatgcatgat ttttcataca attatttccg tgaaatagtt
     3301 aaagaagatg aaagatacaa tcaattatcg gatgaatatg tcgatgctct gcatactggt
     3361 acatgggtag cagtgactgg cttagcgtca ttgatgtcat caaacatcat gcatccaaca
     3421 gaagaacaaa tcattgcact gatcaaacgg acaattgact cgattttagc attagataat
     3481 cctgaaataa tcagatagtg ttctatcagt atgaatttga tcggtaagtc cgttggctgc
     3541 aaactgtcaa tggacttttt tgctaaaaaa agcgatttct gttatttatc tattagaaat
     3601 aggcttgaca tttcgtgaaa aaagtttaaa aatagtactt acggaaggtt ggcagagtgg
     3661 taatgcaccg gactcgaaat ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct
     3721 gtaccttcct tatatcaacg attagaggtt gtgaagaagc tgtcctgcat caagtaataa
     3781 gaacggccaa acaaaaatag cttttcctat tttttcgttt gattgggctt attaccgcag
     3841 atgcaagctt ttgaacaccg tttgttcgga ataaggggct gtgacaagac ttttgtcaca
     3901 gccccttatt gttgtacttt taacctacaa tattttttga ttccccctga gtactatttt
     3961 ttataaatgc tagtaaatca atcttctttg gccttttttt gataaaaaaa atactagatg
     4021 taagataaaa gtgtaaaaac gatttgtttt aggaggggaa acgatgtgta ccgcaatcac
     4081 ttatgcaacg aaagaccatt attttggaag gaattttgat tatgagatgt cttataatga
     4141 agtggtcact attactccaa gaaattatcg tttcgatttt agaaaggtaa aaaatttaga
     4201 taaacattat gcaatgatag gaatagcagc tggggtatcc aactatccac tctattatga
     4261 ggctacaaat gaaaaagggt tgagtatggc aggactgaat tttcctggca atgctgatta
     4321 taaagaatta caagaaggaa aagataacgt tgcaccattt gagtttattc catggatact
     4381 agggcaatgc tcaaccatag acgaagctaa agaattattg gctactatca atctagtcaa
     4441 tatcgatttt agtgaaaaac tacccttgtc acctttacac tggttattag ctgataaaga
     4501 aaaatcgata gtaattgaaa gtatgaaaga tggacttcat ttatatgata atcctgtcgg
     4561 tgtgttgaca aataatccgc catttgatta tcaattattt aatctgaaca attatcgctc
     4621 gctatcaaat ggaa
//
LOCUS       DAJSAW010000115         4608 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJSAW010000115 DAJSAW010000000
VERSION     DAJSAW010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.119"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(83..337)
                     /locus_tag="ONT64_002731"
     CDS             complement(83..337)
                     /locus_tag="ONT64_002731"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2304098.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(448..702)
                     /locus_tag="ONT64_002732"
     CDS             complement(448..702)
                     /locus_tag="ONT64_002732"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2304099.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(895..1170)
                     /locus_tag="ONT64_002733"
     CDS             complement(895..1170)
                     /locus_tag="ONT64_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HCR2304100.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1142..2095)
                     /locus_tag="ONT64_002734"
     CDS             complement(1142..2095)
                     /locus_tag="ONT64_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR2304101.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2707..4200
                     /locus_tag="ONT64_002735"
     CDS             2707..4200
                     /locus_tag="ONT64_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HCR2304102.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4335..>4608
                     /locus_tag="ONT64_002736"
     CDS             4335..>4608
                     /locus_tag="ONT64_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HCR2304103.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          801 g         1541 t
ORIGIN      
        1 cttgaattct tgtgaaaaag ttcttcgtgt tctcttagac ataaaaattc ctcctggtat
       61 gttttcttct agtctacaca ccttaatttt tctgtctagt taattgtagc ctatccatga
      121 tcggtttatt agacctttga ggtctatatt ttttaactac aatcgacctt aaatttaatt
      181 gtttcattag cttttggaca tgtttaagag atactgaaat cccttctttt aataaaactt
      241 gatggatctt tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct
      301 ttttcaactt attatttctg atttctgtag ctgtcaacgg tttattctta taaaaataat
      361 agacacttct agggatttct aataaccgac acaacaaaga aacagtgtaa tccttacacc
      421 atttttcaat aaattgaaca agcgaattta ggttttccga gagaatatgg ctgcggcttt
      481 ttttaagatt tcaagttcct catttaaacg agcattttct tttctcagtt cagctacttc
      541 ttttccagtc agtcctgtgg actgatcagg taaatataaa ttcttccatt tgtaaatcgt
      601 tgcttcagat aagccatatt ctttcgctaa ccctcgaaca gaacgtccag tttgattcag
      661 ttcaacaatc atttgtttaa aattttcttc atatcgggtc ataaaaattc atcctctcgt
      721 agataagttt aatttattct tgtctctacg aaaagctgtc taagatttga tactaacacc
      781 aaatgttgta tacccccaaa caatacacat attttgaagg attaggatta ataaagctaa
      841 aaatatagta agtatactaa atatatttac tatatctagt atacttacta tattttaatc
      901 ttttgatcgt ctattatata taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc
      961 cattgcaagt ttgactattt catacatagg aatatctttt gcataactaa gatcccgaat
     1021 ggtttttagt acgggaggat ctacttgaac tggttttctt tctttcgcag tgtactttcg
     1081 ttcccctaac tcgacattaa tatcatcttt aattgaaata tcttcagcac caattttctt
     1141 cttatctttg ttcgttggta ataatgccat ttagtttaat ctcctttcca agaggtagga
     1201 tatgattggc cattgagtca gcatatttag gtgtataatc aaatccttca atatcaccat
     1261 gttgttcata gtattcaatt ctttcttcta attctgtaaa gatgtctgca aaaatagccc
     1321 accatcttcg atctacataa tcatttaatt ggataccatt ctcaccatac atttctaacc
     1381 gatccgagcc tttaacaatg cttttaaaaa tgtttgattc accatactta tcaattgttt
     1441 tttgatagtt tgcgtgttga gaggaacgtc gcactgagaa aagaacaggc aaaattccaa
     1501 tgatttgaag atgagactct tcaaaattcg tgacaatcgg aactaaaact ttattaataa
     1561 aatcttctgt tccttccatt gcgtaccgtt ttaattcttg cattgcaata atataatctg
     1621 cagcagttaa gaatgcacga accacattat ctgttgatgg tgggcagtcg ataaagatat
     1681 aatcgaaatt ttttctcaat tcttctaaag gtttaatgaa ggccaatgat ctcttacgac
     1741 gatcaggata tttttcggac aagtattcat taagttcttc agatccagga gagccagcca
     1801 taatatataa atgtggactt acttgagtaa tacctgaaac ccagtcccct tttttgattg
     1861 ctgcagacat ggatcgatca aagctagtaa tttgatacgt tgatccaatg ttggaagtag
     1921 tatttctttg ttggtcataa tcaattagca acacgtcttt ttcaaaaagt tgactagaaa
     1981 caatcgctaa taaatcattg tctgtagtct taccaatgcc gccttttcta cctgtaagca
     2041 ttacaacttt tcctagttta tctgatgttt taaatgcagt ccgtaagttt tccatagttc
     2101 cctccaattt cctatactaa atatatttag tatacttcat atacttaata tattcgatat
     2161 atctaatata ctccaaattg ataagaattc agactttata aggaatatag tggatatact
     2221 aaatatattt gctatattca gtatatcaga tatattcaaa atgtcaaaat atatttgtgc
     2281 gttttgcgtt gacaatttag agtgaaaaga ggtaaactta attctataga aaccacagat
     2341 aattcactat ttacaaagta gcaaaagcag tggtactctt taaacagaaa aattacataa
     2401 aaaaaagaga cggaaataat tgattttagc agaatcaatt atttctgaat cacatggtta
     2461 ggacatgtgt tcacgctcgt ctcaagaata tgtaagtagc taccaactac ttatattaaa
     2521 agttgatcgg ataccttttc cgattgcctt tattctacca aaaaaaggcg agcaactcaa
     2581 cagaagaacg tttatttagt gtacttaaat taaatattct taaacactga agagagaaca
     2641 catactatgg ctgagattaa agctaaactg tgttctcttt ttgtttgaga aaggacgtta
     2701 caatcgatgt tgagcgaaat ttattcagct attcttaaaa acggcttacg tacatacaaa
     2761 tatcgaaaca gcaccatgaa acctgcaggt catattgatt ccagtaggag tggtgctatc
     2821 tttgcgtacc gtagcaagca attaatgaat gttgggcgtg gtatggtcat tacttcggaa
     2881 gaagctattt tagagaacga aaagaaacta acgcattgga cacctaatac ctatcgcttt
     2941 ggtacttatg cggatgatta tcgaacagtg gttaaaggtc attctgagaa aaacttaagt
     3001 caaatcaaca catttgtagt tgatatcgat tcaaaagaga accatcaagg cgaaattatt
     3061 ttagcctgtt tagatcaagt gggctacatg ccaacgctaa ttttagaatc agatcatggt
     3121 tatcaagttt attttgtact aaagacacca ggctatgtca caaagaaaag caatttcaag
     3181 gtgatcgatg tagcaaaaaa aatttcaaaa acaattcgtc tacagttggc tgaaaaattg
     3241 tccggagtgg atctaggatg taaccacttc ggaattgctc gattcccaca agaaaataat
     3301 attgttttta aagaaatgaa ctatcagtat tcttttgctg actggattca atggtcgatg
     3361 aagcttgaat cagatcaaaa aacaaaagaa gatcgagaac gaaaactaat tgtctttcct
     3421 gagaaaaaag aaattcgtca agtagatgaa ccgtggttcg atatgttact tcataaagca
     3481 aacatcgtcg gcggagaagg acgtcttggt cgaaataatg tgattttcac gctttcttta
     3541 gcttattact cttcaggtta tgcattggat acttgcgaat acaatatgtt cacattcaac
     3601 gaacggttac aggaaccgct atctgaagca gaattagtaa agattgtacg tagtgcttat
     3661 tctggtaatt atcaagcagc aagcagagaa ttcattattg aattgtgcca gcaatgggtt
     3721 tcaccaacct taaaaaataa tgatctgttc attcaacgac gtggctggtg gaaatttaaa
     3781 aagccacgag atcaaagaaa atatagtcat aaaaatgaat ggaaagaaga tctattggct
     3841 tatctaagcg aaaaaagtct tgctagtgag ccttatttga acctgtcaaa aaaagaattg
     3901 gcttcacaac taacgatgcc caagcgaaca cttgataaga tcttaaatga gttacgagag
     3961 gagaataaag tcttctacaa ggtaacacga ggaagaaatg gcggaatgat cattgcttca
     4021 gtcaaaacgt tgttcgcgag ggttattcaa ctaagtaaag aaaagagact agattattca
     4081 acaaggatca cagaggtatt tggactcaac gagagcttag tagaagagac atttagacaa
     4141 ttatcaaccc ccttgaaccc tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga
     4201 aaaataaaag gtgcaaattg ccttatacaa tatatctatg atacttctgt aggtgttgct
     4261 ccctttgcct agctacgtgt aggtggtata cttataactg cgtatgcagt cttatgtatc
     4321 atggaggaag ttgtatgtcg ttaaaaaatt atgtctattc acatcctgtt aatgtgttta
     4381 tcattggtcg tttgggatta tctgtggaag tgttctgtga gctttatggt tttaagcagg
     4441 ggactttgtc ttcttgggtt accagggaaa aaacagttgc ttctttgccg attgagtttc
     4501 ttcatgggtt gtccttagct tctggggaga ctatggatca ggtatatgat tgtttgtgtg
     4561 tgttagagca ggagtatatt gagtataaaa tagctaacga gttaagaa
//
LOCUS       DAJSAW010000116         4484 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJSAW010000116 DAJSAW010000000
VERSION     DAJSAW010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.120"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            439..1350
                     /locus_tag="ONT64_002737"
     CDS             439..1350
                     /locus_tag="ONT64_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HCR2304104.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1837..2103
                     /locus_tag="ONT64_002738"
     CDS             1837..2103
                     /locus_tag="ONT64_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304105.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2175..2516
                     /locus_tag="ONT64_002739"
     CDS             2175..2516
                     /locus_tag="ONT64_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304106.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2571..2990
                     /gene="ssb"
                     /locus_tag="ONT64_002740"
     CDS             2571..2990
                     /gene="ssb"
                     /locus_tag="ONT64_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR2304107.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3067..3363
                     /locus_tag="ONT64_002741"
     CDS             3067..3363
                     /locus_tag="ONT64_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304108.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3378..3809
                     /locus_tag="ONT64_002742"
     CDS             3378..3809
                     /locus_tag="ONT64_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCR2304109.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            3991..4296
                     /locus_tag="ONT64_002743"
     CDS             3991..4296
                     /locus_tag="ONT64_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304110.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT         1652 a          691 c          950 g         1191 t
ORIGIN      
        1 aaatttaaaa gggaaaaaat caacccctac cccagtcttg gggtctcaaa actaactccc
       61 ccctaacccc ctctttcagc ctcttttgaa agagggggag aatcaacccc acggccaacg
      121 ccccagtgtt cttggggctg tccagaacag aaacttgcga gagagtgggg gtaaaacaag
      181 tgtagaggaa acttgaagac ttgttcgaac agaaacttgc gagagagccg gagtaaaaca
      241 agtgtagagg aaacttgaag acttgttcga acagaaactt gcgagagagt tggagtaaaa
      301 caagtgtaga ggaaacttga agacttgttc gaacacaaac ttacaagaga ggggagtaaa
      361 gcagttgcag aggaatcagg aagatttgtt cgaacagaaa cttgcaagaa agcattatta
      421 aattgaaggt gatgaaatat gaagtattta ttgcatggct attttattga ttattctact
      481 cgcaatagta gtactttagc gattctatgt ttgcttcatg aattgagagt ggttactaga
      541 aaacagctat acaaactatt gaattttgac agatcaatta gtaaacaagg agtaacgaat
      601 gctttagtga gattatatga aaaacagtta atcgatcgaa aaaaatatgg aacagaaatg
      661 tattattatt tgacgaaaga gggacatcaa agcataggtg ggtattatac gttgccgaaa
      721 gttcctgaat ataatttgaa tcaccattta caaattaaca attatttgat taaaggaatg
      781 gcattggcaa aagaccaccc acatttaaaa cggattattt cagaaagacg taccgtctat
      841 gaaacgaagg atttaaatcc taagacaaaa ggaagaaaat tttttgtgcc agattttatt
      901 tttcgttttc gagacaaaga aaatcatgag atcaattggt cctttgaaat tgagctaacg
      961 ttgaaaacac gtcgtcgcta ccgtgaagcg atttttccga aatatattcg tgagttgcga
     1021 acacgtcctg aatcacattt aatttatgtg acacctagtg gcgcaattca agaagaactt
     1081 catgcgttta agcgtatttt tttcaagcgc gaaactgaaa aagatgcgag tcaacaagaa
     1141 ttgtttgagc ggttgcatat tttttcagct ttagagtttg aagagggatt aaaagcatta
     1201 ttagagaatg atccagcagt caattggcgg acagtagaac gagaaaaaat ttatccgaat
     1261 ggcgctgttc aaagtcgttt gaatttattt gaagaatcaa ctgaaaatga ggaaaaacca
     1321 aaagtgaagt ttacgcctga agtattgtaa aagagggtgt tcgcttcgct ctttttttgt
     1381 gccagtttgc tggcaccgtt cagacgcaag cgtctgaacc ggctcgtttt taggaaggac
     1441 taacttgttt gatttatccg ttgtcaaaga agtggacgtt cgagagtccc taatcgtata
     1501 gcagtcattc ggcgagtata acctaaaaca taacctgcca attgccaagg gtaaaatgca
     1561 cgctgcgcgc ccttggcaat cgtcagaaca tgttttcgag ccggttatgc cgaacgaccg
     1621 ctatacgaaa agggcctcta aacagaagtc cactttgatc cttgacaact gaaacccaaa
     1681 atcaaacaag caggccttcg tctaaaaacg agccgaatga agaccttggg ggttgcaccc
     1741 ccaaaccccc gtgcccccga cacactaccg aaaaggtggg ggtgggtggc gaaaaaatca
     1801 aataaaacat tctattcaag aaaggaagaa acaaccatga caactaaaaa cgagcaactg
     1861 aaagaacagg caagaaaaga gttgcaagaa agaggtttga ttattgaggg gagctttgaa
     1921 ggagattttg aaacctatat tggttgttat gcaagaccga taaacaaacc tacagcctta
     1981 gacccaatga acgaacaaga agtaaaagaa caagaaaaac atgcagtgaa tggttttccg
     2041 caagatttca ctgaatggta tgagtgggaa attaacaacg ggaagcttga aaacttctta
     2101 taaaagtcag atgacgtgca aagagaatga ttctctttgt acggtaaaaa tataaagaaa
     2161 gaaggaaaaa gaaaatggca aaaaacacac cttatcaagt aggagacatt ttttatagta
     2221 gttggggcta cgaacaaacg aacgtgactt tttggcaaat tgtgaagtta acagaaaaaa
     2281 cagcatggtt tcagcctatt caaaagaaaa cggtcgagca ttattcgtcc atgagtggaa
     2341 aagttgttcc aatgaaagac aagtttatag agtatccttt agcaaaaact aaagacagta
     2401 ttgtaaaaaa acgaattgat ccagaaacct atcctgatat gttatgtggt tatcgtagtt
     2461 gggacacgtt tcatagatat aaaaatcaag ctgtttatga aagtagttat tattaagggg
     2521 atagtttccc ctttcaaaaa taataaatag aagaatggag agaaaaaatc atgatgaatt
     2581 cagtgaattt agtaggacgg ttaacaaaag aagtggattt aaaatacaca ccaaaaggca
     2641 atgcgacagg cacatttatt ttagctgtta atcgaaatta tacgaatgca aacggcgagc
     2701 gtgaagcaga ttatatccgc tgtgtcattt ggcggaaagc tgcggaaact ttagcaaaat
     2761 atactggaaa aggaagtttg attggcatta atggacgtct tcaaacgagg agctatcaga
     2821 atcagcaagg acagaccatg tatgttacgg aagttctagt gacagatttc tatttgttag
     2881 agagtaaaga aatcaatgag caacggtcaa aatcaatggc gaataatcaa aataattctt
     2941 caattccagg aaatgagatt caaatttcag atagttattt gcccttttaa gtgaaaaata
     3001 attttgttct atacctgatt tcttcaggta tagaacaaaa aataaataga gatgaggcga
     3061 ttttttatga aaatcattga cagttacgat caacgaacca tgcaatattt gggctcgttt
     3121 gattatacag atgaaaatat tattaattat gttgcaagtt tacctcctta tcaaagcgtt
     3181 cgcttggtag attttgcaag tgatgaattg gtattaacaa cgatgggcaa ctttttagat
     3241 tatgtcggag acaaaaaatg gttggaaaaa attttgtcta aattgattgc gaaacaaaca
     3301 ggagaagaac ccataaatgc gattcaattg ttcaatcgat atgaacaaga aaacaatgat
     3361 taaagaggag agaaaaaatg aaaagattaa aacgaagtat ttttattgtg ctagcattga
     3421 ttgattttgt tgggacaggt tgtgaagcaa ttgacgttca aaaagctccc ttatcggaaa
     3481 taaaacaagc aataacaaaa caagatgaag aacaaccgct aaatacacaa gtagaaatag
     3541 tgaatcaaaa taaaccgagt ttttctgaag aagatttttc gttagaaaat ggaaccaggc
     3601 aatttttttc agatttggat cgtttaaatc gggtaggtgt agcaagtgca cttttgcata
     3661 aaagcaggat gccaacagaa gaacgagacc gattatacat taagccaact ggctggaaac
     3721 aaaagaaaat gaagaatggc gattatcttt ataatcgttg tcatttgatt ggctatcaac
     3781 gtgcgacaag tttccccata agtatgtaat acgaaacggg aggattaatc ttaagattaa
     3841 tcacaactaa cctatttgac agttggaatg aaagctatca tgtctgctga atcaactggt
     3901 ctgatactcc tacatgcaat aaagtctact aggaagttat tgtatgaagc taggtaaagg
     3961 cggcagaaca atttctaagt gtggaatcat atgaagttgg atatgccggg acgctataaa
     4021 ttactcaggg tgagactgtt tttaattaaa ctgacgaact tgcgaatgta cgggtctata
     4081 accgacttat attcgaaaga tataagattg cgaatgcaaa gaacattatc gaaaagtaag
     4141 aatgttggtc atgaaattcg agatggaaga ctagcggtgt atctgtcaag agttggtaaa
     4201 acatgtttga ttgattcaga tgagagtggg aatgcgacaa aacgttttac agaagaaata
     4261 gagcaactag cagaactgaa tatacgatat ttttaaatac tttagcggag aataaaaaaa
     4321 tcctctacag aaaactacgg agggcaacat gtgctattgt ttcaacaata catttttaat
     4381 ttttaccata acaaaaaagt acttgaaaaa aaacagaaat atgttaaatt aaaaatgaaa
     4441 aaatggaagc ggttacttat tcttttgaat ctaagataaa aatt
//
LOCUS       DAJSAW010000117         4475 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJSAW010000117 DAJSAW010000000
VERSION     DAJSAW010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.121"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            323..1054
                     /locus_tag="ONT64_002744"
     CDS             323..1054
                     /locus_tag="ONT64_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR2304111.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1199..3226
                     /locus_tag="ONT64_002745"
     CDS             1199..3226
                     /locus_tag="ONT64_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCR2304112.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3334..4431
                     /locus_tag="ONT64_002746"
     CDS             3334..4431
                     /locus_tag="ONT64_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCR2304113.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1574 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
       61 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
      121 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
      181 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
      241 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
      301 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
      361 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
      421 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
      481 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
      541 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
      601 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
      661 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
      721 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
      781 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
      841 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
      901 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
      961 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
     1021 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
     1081 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
     1141 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
     1201 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
     1261 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
     1321 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
     1381 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
     1441 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
     1501 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
     1561 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
     1621 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
     1681 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
     1741 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
     1801 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
     1861 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
     1921 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
     1981 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
     2041 gcaaagaagt cttacttatg atttggaagg tgtcggtcac tcttctcgga ttatacctga
     2101 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
     2161 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
     2221 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
     2281 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
     2341 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
     2401 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
     2461 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
     2521 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
     2581 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
     2641 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
     2701 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
     2761 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
     2821 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
     2881 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
     2941 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
     3001 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
     3061 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
     3121 tacagctcat cgtggaatca gaatagatat tcctgtagaa aaccaaagaa tcggaacctt
     3181 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
     3241 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
     3301 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
     3361 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
     3421 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
     3481 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
     3541 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
     3601 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
     3661 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
     3721 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
     3781 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
     3841 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
     3901 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
     3961 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
     4021 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
     4081 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
     4141 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
     4201 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
     4261 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
     4321 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
     4381 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
     4441 aacaaatagt aaatttttat tattgtagga tggtt
//
LOCUS       DAJSAW010000118         4434 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJSAW010000118 DAJSAW010000000
VERSION     DAJSAW010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.122"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(543..2048)
                     /locus_tag="ONT64_002747"
     CDS             complement(543..2048)
                     /locus_tag="ONT64_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2304114.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ONT64_002748"
     CDS             complement(2045..2731)
                     /locus_tag="ONT64_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2304115.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ONT64_002749"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ONT64_002749"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR2304116.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1547 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DAJSAW010000119         4425 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJSAW010000119 DAJSAW010000000
VERSION     DAJSAW010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.123"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            690..1658
                     /locus_tag="ONT64_002750"
     CDS             690..1658
                     /locus_tag="ONT64_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR2304117.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="ONT64_002751"
     CDS             1669..2484
                     /locus_tag="ONT64_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR2304118.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ONT64_002752"
     CDS             2552..3262
                     /locus_tag="ONT64_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2304119.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ONT64_002753"
     CDS             3298..>4425
                     /locus_tag="ONT64_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCR2304120.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAJSAW010000120         4345 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJSAW010000120 DAJSAW010000000
VERSION     DAJSAW010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.124"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(387..1853)
                     /locus_tag="ONT64_002754"
     CDS             complement(387..1853)
                     /locus_tag="ONT64_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2304121.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="ONT64_002755"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="ONT64_002755"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCR2304122.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1412 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaa
//
LOCUS       DAJSAW010000121         4339 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJSAW010000121 DAJSAW010000000
VERSION     DAJSAW010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.125"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            127..579
                     /locus_tag="ONT64_002756"
     CDS             127..579
                     /locus_tag="ONT64_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304123.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ONT64_002757"
     CDS             593..1282
                     /locus_tag="ONT64_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304124.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ONT64_002758"
     CDS             1407..3758
                     /locus_tag="ONT64_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HCR2304125.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ONT64_002759"
     CDS             3838..4233
                     /locus_tag="ONT64_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304126.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1528 a          548 c          867 g         1396 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaat
//
LOCUS       DAJSAW010000122         4310 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJSAW010000122 DAJSAW010000000
VERSION     DAJSAW010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.126"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(65..1285)
                     /locus_tag="ONT64_002760"
     CDS             complement(65..1285)
                     /locus_tag="ONT64_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HCR2304127.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1916)
                     /locus_tag="ONT64_002761"
     CDS             complement(1305..1916)
                     /locus_tag="ONT64_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR2304128.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="ONT64_002762"
     CDS             complement(1959..2891)
                     /locus_tag="ONT64_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCR2304129.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3312..4172)
                     /locus_tag="ONT64_002763"
     CDS             complement(3312..4172)
                     /locus_tag="ONT64_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2304130.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1230 a          866 c          707 g         1507 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tttatatatt tttttatata ataaggtaat tttaatataa aattttatat tgtaaaaaaa
     3061 caaataaggg aaacaaacaa cacatttttg tgtgttgctt atttccctct aactatcgtt
     3121 ttctatttga tggttaacct acttaaaata aatactgcaa cgacaaacgg agatcaagca
     3181 tgctttgtaa cgtattttac gataattacg actttatttt attactctat cttgctgcca
     3241 tacccgtcta tccctaatca tctttggtga attttttcaa agttagcata acaaaaataa
     3301 gtattttttc cttagttaat caatagtatc atttttttca tatgatttaa ttcatcaatt
     3361 aattttttgt ttttattaat tttagcaaaa gctagtgcat cttgaatcaa ttcgagcact
     3421 tctgccttcg acttattttt agcctgtgcg ttcttcgcta attgggcagc agctcgtgct
     3481 aagtaataag tcaaatggtt atccgaacaa atatcgattg cgtaatttag tagtgcatca
     3541 cttgtttcca aatcaccgtc ttgcgcaaaa aaaacaccac aatgaaagac gatatttaac
     3601 acccgccaga tatcttctgt tgtacgaatc ggatacgtat aaattttttc aaacactcga
     3661 ttgaaataaa cttctgcttt ttccaactct ttttctctag catagaccat tccaatacct
     3721 gtatatgcca ataaacgaaa caacgtgttg gctgtttctg ccatcaacaa ctgatcaaaa
     3781 tggaacaaca catccgtaat tggtgcattc gtaaaaatca tttcaaatcc ttttagatag
     3841 tagtaacgtg caatcagctc agactcgtca attaccttgg gatcaatggc tgctaaaatc
     3901 tgtgtcaatt gttcatattc actcgtaatc agtgaaaact ctgctttttc tagctgttca
     3961 tcaagttgtg tattgatcac gttcatcgaa gaaaaaagtt caactaacgg aagattgatt
     4021 cgattacata atttgattaa aatttttaac gaagggactt gaccattgtt ctcaaattta
     4081 cttaatgttg cttgtgtaca aattccttca gacacttctt tttgagataa gttggcagcc
     4141 tttcttgccg taataaattt ttcaatgttc attattttct ctccaatcag acctcacacg
     4201 aaaatcattt ctgtctcgat tttagcatgt tttctttggg aatgctttag gttctgttgc
     4261 aaagttttaa ataaagaata aagtccctta cggtatctat gatttaagct
//
LOCUS       DAJSAW010000123         4273 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJSAW010000123 DAJSAW010000000
VERSION     DAJSAW010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.127"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            798..1052
                     /locus_tag="ONT64_002764"
                     /pseudo
     CDS             798..1052
                     /locus_tag="ONT64_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1122..1634
                     /locus_tag="ONT64_002765"
     CDS             1122..1634
                     /locus_tag="ONT64_002765"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2304131.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1631..2668
                     /locus_tag="ONT64_002766"
     CDS             1631..2668
                     /locus_tag="ONT64_002766"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2304132.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
     gene            2828..3004
                     /locus_tag="ONT64_002767"
                     /pseudo
     CDS             2828..3004
                     /locus_tag="ONT64_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            3059..4273
                     /gene="aph(2'')-I"
                     /locus_tag="ONT64_002768"
                     /pseudo
     CDS             3059..4273
                     /gene="aph(2'')-I"
                     /locus_tag="ONT64_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
BASE COUNT         1609 a          639 c          818 g         1207 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc taaagagaat taagttttga
      541 ttatgtaata agttttggta ctaaaaaaga cttgatctaa ttagaccaag tcttttgtta
      601 gtgttatatt aataacaaaa taaaaataag aagtcgctca ctccccgacc aaagtttgtg
      661 agcaaaataa acagtcgatg tttgtttctt aattgtatat cttgatatta aacgatttaa
      721 gattattctt caagatatat attcgcaaat gtaattggaa aagcgacttc ttaaacaaaa
      781 ttaaggagtc gctttttatg gcaaaaataa cgaaaaatga gcaagaaaat ttggtggaaa
      841 attacacaaa aaaaccggcg tactctaata gccggttaga cgcacatttc gtgtgcactt
      901 ctaatccgaa attgagcttt gacgctatga cgatagtcgg aaaccttaat aaaaacagcg
      961 ctaaaaagct atccgatttt atgagtttag atccacaaat tagactttgg gatatacttc
     1021 aaacaaaatt taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag
     1081 gataataagt tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg
     1141 aacgttactc taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata
     1201 gggggattcg ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc
     1261 gtaaaaaagc tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat
     1321 ggtcaagtga agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac
     1381 ttgcggaata ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa
     1441 cgtgcttatc tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa
     1501 aagaagagaa aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat
     1561 tagcggaagc cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc
     1621 aagaggaaga atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc
     1681 caatcgcaat ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac
     1741 ctacgaacga tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg
     1801 tcctgtaccc aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc
     1861 aaaaaaagaa tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc
     1921 gatttatatt gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca
     1981 tcaccgtggc cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa
     2041 gccaaatgaa gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt
     2101 ttaccggtta tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg
     2161 ggaatcagaa gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg
     2221 tatccaagga cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac
     2281 ttttcaagtc ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag
     2341 taatgacaat ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc
     2401 caataacggc tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg
     2461 gtataacgat tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga
     2521 aggacgacat ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg
     2581 ccatcctgaa cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt
     2641 gaatccttta aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa
     2701 ccctcgtcac ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg
     2761 accataggga ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac
     2821 aaacaccgac catcgtccag taaagagacg caataaattc tatcgaagtt tacgcactgc
     2881 ctctacaacg attaaaggca tggaagccat ccgaggatta tataagaaaa cccgaaaaga
     2941 aggcactctc ttcgggtttt cggtctgtac tgaaatcaag gtattattgg gaatcccagc
     3001 ttaaatcata gataccgtaa gggattttat tctttattta aaactttgca acagaaccga
     3061 atcattaaaa aaacattata cagagccttg ggaagatgaa gtttttagag taattattga
     3121 atataacaat gttcctattg gatatggaca aatatataaa atgtatgatg agttatatac
     3181 tgattatcat tatccaaaaa ctgatgagat agtctatggt atggatcaat ttataggaga
     3241 gccaaattat tggagtaaag gaattggtac aagatatatt aaattgattt ttgaattttt
     3301 gaaaaaagaa agaaatgcta atgcagttat tttagaccct cataaaaata atccaagagc
     3361 aataagggca taccaaaaat ctggttttag aattattgaa gatttgccag aacatgaatt
     3421 acacgagggc aaaaaagaag attgttattt aatggaatat agatatgatg ataatgccac
     3481 aaatgttaag gcaatgaaat atttaattga gcattacttt gataatttca aagtagatag
     3541 tattgaaata atcggtagtg gttatgatag tgtggcatat ttagttaata atgaatacat
     3601 ttttaaaaca aaatttagta ctaataagaa aaaaggttat gcaaaagaaa aagcaatata
     3661 taatttttta aatacaaatt tagaaactaa tgtaaaaatt cctaatattg aatattcgta
     3721 tattagtgat gaattatcta tactaggtta taaagaaatt aaaggaactt ttttaacacc
     3781 agaaatttat tctactatgt cagaagaaga acaaaatttg ttaaaacgag atattgccag
     3841 ttttttaaga caaatgcacg gtttagatta tacagatatt agtgaatgta ctattgataa
     3901 taaacaaaat gtattagaag agtatatatt gttgcgtgaa actatttata atgatttaac
     3961 tgatatagaa aaagattata tagaaagttt tatggaaaga ctaaatgcaa caacagtttt
     4021 tgagggtaaa aagtgtttat gccataatga ttttagttgt aatcatctat tgttagatgg
     4081 caataataga ttaactggaa taattgattt tggagattct ggaattatag atgaatattg
     4141 tgattttata tacttacttg aagatagtga agaagaaata ggaacaaatt ttggagaaga
     4201 tatattaaga atgtatggaa atatagatat tgagaaagca aaagaatatc aagatatagt
     4261 tgaagaatat tat
//
LOCUS       DAJSAW010000124         4203 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJSAW010000124 DAJSAW010000000
VERSION     DAJSAW010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.128"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(1..354)
                     /locus_tag="ONT64_002769"
                     /pseudo
     CDS             complement(1..354)
                     /locus_tag="ONT64_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(614..1162)
                     /locus_tag="ONT64_002770"
     CDS             complement(614..1162)
                     /locus_tag="ONT64_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304133.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1163..2020)
                     /locus_tag="ONT64_002771"
     CDS             complement(1163..2020)
                     /locus_tag="ONT64_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR2304134.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2306..2592)
                     /locus_tag="ONT64_002772"
                     /pseudo
     CDS             complement(2306..2592)
                     /locus_tag="ONT64_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2582..2911)
                     /locus_tag="ONT64_002773"
     CDS             complement(2582..2911)
                     /locus_tag="ONT64_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR2304135.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2988..3260)
                     /locus_tag="ONT64_002774"
     CDS             complement(2988..3260)
                     /locus_tag="ONT64_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR2304136.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3260..3523)
                     /locus_tag="ONT64_002775"
     CDS             complement(3260..3523)
                     /locus_tag="ONT64_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR2304137.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(3629..4045)
                     /locus_tag="ONT64_002776"
     CDS             complement(3629..4045)
                     /locus_tag="ONT64_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304138.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            complement(4051..>4203)
                     /locus_tag="ONT64_002777"
     CDS             complement(4051..>4203)
                     /locus_tag="ONT64_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR2304139.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
BASE COUNT         1225 a          851 c          533 g         1594 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat acttatttga gagtcatcta attggctcat
      241 aatcctttct ttttggttag tatctgcagt tacttgttta gtatgagcca tgaacccaaa
      301 catactcacc ccaacaacca atcctaaata tattttcttt ttaaactttt tcatctacat
      361 ctcctcctaa tagttttatt ttagagttag ctctattact tatttactaa ctcaacaaca
      421 gattatcata cacttgttaa tgaaatcaca atatctcatt aaaacacacc ctaaaatcat
      481 aaccgttcct atgctcattt tttggatttt ttaatcacgt aaccagaaaa ataaatcccc
      541 tctatttcac gcaaatcaaa gaagaactaa gtaactaccg tttaaaggta gttacttagt
      601 tcttctttga ttattatcta actttattat tcattcccca ccctttgcgc agtgagtctt
      661 cggtatcttg ttttatctga ctggtagttc ttattatttc tccacgatca tctaattctg
      721 gaacaattgg atatttttct ctgaacaagt ctagttcttt caccacgtca ttaaacaaat
      781 cttgcttatc ttcttgcgta agattctgtt taatatactc atctaaagct tttctaagca
      841 attcatctct tgtcatatta gtctcatact tttctactaa aactctggaa gtcgcattta
      901 acaaagtcga cagttttaaa ggatgcttgg tggaataaac cttatcaaac tttctgattg
      961 gtgcgccaac tttttttgtt cttcctctac tctgtctttc aacattggag gaagttttta
     1021 tttcttgaac ttggtgcact tctaattgct tattttcaga tggtgtaatt gcttgctgct
     1081 ttttctttgc ttctgcttct ttttttattt tggcgagtcc tttgttgttc ccaaaaatat
     1141 caagtccttt gatgtcagcc aattactcca cctccgcata ccaattctcg tgttcttcca
     1201 attctttcaa aatactgata aataattcgt ggcttttgcg atcccattgt tttaagagac
     1261 ctttctttgt cttctccatc gtaatacctg atccgtcgta tcgttttaag cgcgcatttt
     1321 tgagtacaac atttttaatc aaatgttccc cataaatctc ttgggcttgt ttatacattt
     1381 cctgatctac tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca acatataaat
     1441 ttgtatcaaa ttctttcaca aaaaagtcca tatactcaag atattgcatc gcatttctca
     1501 tcgattttac ttgggtctgt aagacgacga ttgcccaatc cgcaatgtac atcgcggatt
     1561 tactatatac agaaatagaa ggtgggacat ctaaatagat gacgtcaaac tcatcaaata
     1621 atggttctag cttttctttc atatatttga attgggataa tgaatcatta tattggtctt
     1681 tgatccaatc atcatacatt tcaaaggaca tgtctgctgg aagtatatag aggttattga
     1741 ttactttata gacagcctct ttaggtggaa gattgtttat caacacgtca gtcacagtat
     1801 tttcgtaaac aatcggttca ccttctccgc ctccttcttc gttagtgaag ttcatatgcg
     1861 ttaagttcat cacatcagaa gtatttcctt gcatatccaa gtcaattatc aaggttcttt
     1921 ttccttcatt aactgctgat tcaaacccta acatttgcgc gcacgtactt ttacctacgc
     1981 cacctttaaa attaccgatt acatatttct tcgctaccac gttcctacct cttttctttt
     2041 agcaatattc atttgatact attataacat atttattttt agttactacc aaaaaaaaga
     2101 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
     2161 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
     2221 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa aagaagccgt
     2281 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
     2341 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
     2401 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
     2461 tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt
     2521 ctttcaatgg cttctctttt agctttttaa atacttaatg acatgttttg aaggaacgat
     2581 ttcataagcc aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg
     2641 tatcttcagc aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta
     2701 caagaatctc atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa
     2761 aatcataaaa ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact
     2821 ttgcttcaac ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg
     2881 aattgccttg ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa
     2941 ttacttagta aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg
     3001 taatgcgtcc ttgctgaata aatttcaacc acgtgttcat cttcatctac cttataaaca
     3061 acccggtgtt gtctattcaa tcttctagaa tacctacctt catgtgtagg ttttaacttc
     3121 tcaaatgatt gggtagggag atagggatct tcctttaaag tctgaataac cttctcaaat
     3181 tgaggtttca aattagactg tttgattttc tttaaatcag ctttagccga gtttttaatc
     3241 atcacagaat agttattcat tataagttat cccaatcaat ttcatcaaca ttagtaaatc
     3301 cactatcatc tttttctcgc ttacgaacaa cacccaaagt gcctatttgc tcaagtaata
     3361 gcgtttcttt gattgctttc caatcatcca aaccaataag caccgcatta ttttcactgc
     3421 gctcactaat gatttcaatt tccgtatgat tctcatttac atctttcaac aacttataaa
     3481 aatttttccg tgctgagcta ggatttaaga ctgtttttgc catatctact ccccctctaa
     3541 tttatatctc cattctaacg tacttaatta agtacgtaaa ttcgattgtt ttgtttaaat
     3601 tggagtacca aaacagcatt gtaaagtgtt atccgtttcc tttattttta ttaacaagtt
     3661 ttatcacagg cttcatatat ttgattataa cttcatcttc tacgtagata tctgttatca
     3721 ttacgacatc tgtactattt cgattgagta ctttcacttg atctccagca ttgattttcc
     3781 ctctaaatct tttaggaatg aaccaactat acatatgatt agcatcacca aaatgtgctt
     3841 ttatcgcatt ctcaggatat aacttattca atagttcatt ctttttaatt ttcaaaactt
     3901 caaatctata cttatcatca tctgctaatc taaataaatc ttctaaacgc atgttttcta
     3961 actgtaagtt ttgaaaatct tccggtataa attttcggtt ctgttgcgaa gtttgaaaat
     4021 caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt attcccatta ataccttgat
     4081 ttcagtagac accgaaaagc tgaagagcgt tccatttctt cggttctttt tatatattcc
     4141 tcgaatggtc tccatgccct taatcgtgga agaggctgta cggagacttt gataaaattt
     4201 att
//
LOCUS       DAJSAW010000125         4136 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJSAW010000125 DAJSAW010000000
VERSION     DAJSAW010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.129"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1097)
                     /locus_tag="ONT64_002778"
     CDS             complement(<1..1097)
                     /locus_tag="ONT64_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR2304140.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="ONT64_002779"
     CDS             complement(1227..2603)
                     /locus_tag="ONT64_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2304141.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..3259)
                     /locus_tag="ONT64_002780"
     CDS             complement(2603..3259)
                     /locus_tag="ONT64_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2304142.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3269..>4136)
                     /locus_tag="ONT64_002781"
     CDS             complement(3269..>4136)
                     /locus_tag="ONT64_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2304143.1"
                     /translation="IKEGKNIEENDKNSIIVHEEFAKQNNLKLGDEVDLELLDIEKSG
                     KIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDYSTSQEILNNSENNEIANKILM
                     YSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMTYSIMLG
                     GMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQFIFELIFISIPSIISSLFLGNV
                     LLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQSYLILISIIVLSVVFASSLML
                     IKKPKEILSKIS"
BASE COUNT         1297 a          755 c          519 g         1565 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgataaata atctattaaa ttatagtttt
     3001 gaaatactaa agatatatta ttttttctat gattactata tcctttattt tgtatatctt
     3061 cattcttaaa caatattttt cctgtttgag gtttatcaag tccagcaagt aaggaaagaa
     3121 gtgtagattt tcctgttcct gactttccta ctatcgcata aaacttccca agttcaaatt
     3181 tttgatttac tcctgacaaa actttttctt tagaatttgc ataactatat gctacattct
     3241 ttatttctaa tatatccatt attttctcct aacttatttt tgataagatt tctttaggct
     3301 tcttgattaa cattaatgaa gaagcaaata caactgataa aacaataata cttattaata
     3361 ttaaataact ttgtccaagt gttgttatat ttgacataaa actactatta tttattaaac
     3421 ttccaccgga aatcattgaa tcctctgagt taatgaatcc atctacaatt acttttagta
     3481 atacatttcc taaaaataag gaagatatta tacttggtat cgatatgaat attaactcga
     3541 atataaattg cattataatt tgtatcttag atgttccaat agataagaat atacctattt
     3601 cataaattct ttcccttaac catagaatca agattaatga aagaacaacc atcccaccta
     3661 acataataga ataagtcatt acttttatta tatattttat tccactcact gactctaaag
     3721 actcttcaaa cgcattagaa tctttttcaa caaaatactt tgactcatca attttaagct
     3781 ctttcaattt gtttaaggct aagtctgtag attctgcact accagaatac attaaaattt
     3841 tatttgcaat ttcattattt tctgaattat ttaatatctc ttggcttgtt gaataatcta
     3901 caaacaccat attttcacta aaatcagaag ataatcctgt atatgtttcc tgttttttac
     3961 cagaaaagat ccctataatt ttaaatttat gactttttat ttttccactt ttttcaatat
     4021 ctagtaattc aagatcaact tcatcaccta attttagatt gttttgttta gcaaattctt
     4081 catgaacaat aattgaattc ttatcatttt cttctatatt tttaccttct ttaatt
//
LOCUS       DAJSAW010000126         4103 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJSAW010000126 DAJSAW010000000
VERSION     DAJSAW010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.131"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(51..509)
                     /locus_tag="ONT64_002782"
     CDS             complement(51..509)
                     /locus_tag="ONT64_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2304144.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(522..1853)
                     /locus_tag="ONT64_002783"
     CDS             complement(522..1853)
                     /locus_tag="ONT64_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HCR2304145.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1864..3306)
                     /locus_tag="ONT64_002784"
     CDS             complement(1864..3306)
                     /locus_tag="ONT64_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2304146.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3293..4057)
                     /locus_tag="ONT64_002785"
     CDS             complement(3293..4057)
                     /locus_tag="ONT64_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HCR2304147.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
BASE COUNT         1236 a          888 c          604 g         1375 t
ORIGIN      
        1 ttgtaaattc acccataaat atcaccctat ttaacttttt tcatcgtaca ctaaaattca
       61 aaacaactat ctttaatctt tacttcttgg ttttgtttta ataaatttcc ttcaaaaatg
      121 cctggagaaa ctacaatgat caccgtatct ttttgtgctt gctccaactt cttcaaatct
      181 ataatagcta acagtgtccc ttcttttacc tcctggttac tttcaacccg tatctgaaac
      241 ggacttcctt cgagttcaac tgtatctaaa cccatatgga tcagtatttt tctgccagat
      301 tcactttcga tcgttatggc atgtcgggta ggaaatatat tatcgattat tcctttaact
      361 ggcgcatgga tatatccatc atgattcgtt atagcgtatc caactcctat catttttgaa
      421 gaaaagacct cgtcttttac tgctgttaat ggaataattt gaccactagc tacagctttt
      481 agaatgtttc ttgttttaaa gaaatgcact ttttatcacc tctactttaa tgctggccaa
      541 tattttttgt ttactttgat catttcatct agtatttttt ttgcgactct ggcatttggt
      601 acggtttgat tcaatgtaaa tgcttgtaaa gctttttgat aagaaccttc aaagtaagca
      661 tctactagta acttctctac tgcaacttgt gcctccatca atcctttatg aaaatcactg
      721 attggttttc ttagtgtaat tggctccaca ccagttgcac caacatatgc cggtatttca
      781 acaactgcat ctgaccgtag attcggaatc gccccttgat taggaacgat tagcataaag
      841 cgacgacgat catcattcaa caaggaaata gctatatcga cgatatattg tccatgctcc
      901 ccaaaattaa agttcaatac ttcttccaca ataccgcggc gtattttatc agccatttct
      961 ttggtatttt tctctcgtcc atccatgacc atattagcac gtgtatactc tttgtcagta
     1021 tgctcaacga ccatatcagg atataagtaa tactctaagt aattattcgg gatattactt
     1081 ggaaaatttt tcgtgatgac tgacatcatt tgaaaggttt tttgccaagt tttatctcct
     1141 atattaaaat cggcgacttg catttctttt gttattagtt tttcaataat ctctggcata
     1201 atttctcgtt gcaattcttt atcataaatc gatgtatacc agccaaagtg gtttaaacca
     1261 taatacgtaa cgatccaatt cttacgatca taaccgtaat tcactgcaat cgtttcttca
     1321 attgaaatag tcatatcaca ggcattaatc atttttacat tcggatattt acgacgaaca
     1381 gcttcagaaa cgatcgactc tggatttgta taattcaaaa tccatgcttt gggggcatag
     1441 tcttgtatat gatcgacaat ttccaataaa cctttgattg agcgcatccc ataagcaaat
     1501 cctccgagtc cacacgtttc ttgtcctact aatccatgtt taagtggaat cttttcgtcc
     1561 ttttctcgca tctccaatcc tccaacacga atttgtgaga aaacaaaatc gcatcctgta
     1621 aaagcaagct ttggatcatc cgttgcacga atgcgaatat ttgaaaaacc ttttcttttc
     1681 agcataaatt taacaatcaa gtacatatcc atatttcttt cttcatcaat atcatataaa
     1741 cgtatttcag ataatggcaa ccgtttttca ttgttcaaaa tggcttgaac gattcctggt
     1801 gtatacgtac tcccgcctcc agctatagtg ataatctgtt ttttcatcat catcttttcc
     1861 tttctattct tctaataatt tattgaattc tgccataaca taagtcactt ctaagccgat
     1921 gataatttga taattttttt cagttggttt aatgatcccg cttatcccta attgcttgat
     1981 tcgctgttcc tcgaccatgg tgggatccac tacctctacc cgtaggcgtg tattacaact
     2041 atagacatcc aggatattgg cttttcctcc aaacgcttgt agcatttttg ttgccatata
     2101 ttcataattt ttacttgcta accctaactc tgtttcttct aaggttactt cattgccaaa
     2161 ctctgcctta gcttcacgtc ctaatgtctg aatgtttctc ttattgatca aataataaaa
     2221 agtaacgtaa tacaaggcaa aaaagaaaat tccgattgga agaatcaaaa tagcattggt
     2281 agcgattcga aaattgatga gataatcaac aatacatgct ccgaatgtaa aaccaattcg
     2341 tatattgaat aagtaacaga caatccctgc taaaccggca aaaagtgcat ggatcacgta
     2401 caacatagga gaaagaaaca tgaaactaaa ttcaacaggc tcagtgatat ttgcaataaa
     2461 tgaagttcct gcaccgcttc ccatgattcc tttgaccatc tctttatttt ctttataagc
     2521 tgctttatag atagctagac atatagctgg tataccaaac atcattacta caaaaaaaac
     2581 tgataaaaac agtccagcct gaggatctcc gttgataaag cgtgtcatct ctcctctgaa
     2641 aacttccttg ctaggagatg tatagcttcc taaatcgtaa taaatatacg tattcaacac
     2701 atggtgcagt ccaaagggga tcagcaaacg attcaaaaac ataaatatcc ccacacctaa
     2761 tgctcccata tcaacgagca gttttgcaaa attatcaatc ccaatttgga tgagcggcca
     2821 gataaagccc aaaaaaacag ctgcgattgt ttgtagaatc atcaccatag tcaatgggaa
     2881 tttttcgccc gcaaaataag aaaaaactac tggtaatttt tgttctttaa agcgattata
     2941 cgtccatgcc gctactaaac cggaaataat tccaccaaaa atccccatat tcacttctgg
     3001 gtccataatt tctaagcctt ttttcaaggt agcaatcgct aaaaaaccag taagtgcagg
     3061 tattcctttg tccttatttt tgacaaaacc gattgcaact cctatagcaa ataagatatc
     3121 caagtttcca aaaacggcat ttccagctgc accaattact gggatgttca gcatatcgtc
     3181 agctgataaa cgcatgatca atcccgctaa tggcatcgct gcaactggaa gtagcatcgc
     3241 tcttccaagc ttctgcacat ttgatttaac attcattttt attcctcctc tattaaatat
     3301 cctcatatgt tactaactca atcaaaggat catttttcac tttttgagca aatgctgaat
     3361 gaattaaaaa ttcgacttgg tgggtacgag gatacacaaa acttgaacca tttaacaatt
     3421 cctgatccaa atacgctgcg tgacacatga cttcgatcgt tttatcttct ctcaatatca
     3481 tgagtaaact tgccaaatat tcttccacac tcgtattgat ttgtcgtttt ttttcatcag
     3541 tttctcctac gacatcgaaa caaggctcaa aataatcgac atgacgaact tcatcttttc
     3601 gttcaaagtt gctccgaacc ggcaaatcat atttttttgc taatgtaaca actacctcct
     3661 gattttcatc caaagtatga gtatgatgat gactgtctaa atgagttggt atgatccccg
     3721 catgataaat tttttgaatc tgagcattcc attcctgata cagttgatct ttgtcaatgt
     3781 taaatggatg ctgataataa gataaggatc tgaactctcc gctttcgata agagtatcga
     3841 cagttttgag gagtggtttg ccacacgtca atgttaaatg gactcctaca cctagcctag
     3901 gcatttcctt tcttagtttc acagcatgct cgaaacccgg catatttgcc atcaatgtag
     3961 ttgatgtcaa aattcctcgc tgataagcat ccataatccc ataattcaca ccatggctat
     4021 agccaaaatc atccgaatta aaaatcactt ttcccatttc attctccttc ttttttctac
     4081 taaaataggc gcaaccaaat aat
//
LOCUS       DAJSAW010000127         4036 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJSAW010000127 DAJSAW010000000
VERSION     DAJSAW010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.133"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(242..391)
                     /locus_tag="ONT64_002786"
     CDS             complement(242..391)
                     /locus_tag="ONT64_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304148.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            853..1065
                     /locus_tag="ONT64_002787"
     CDS             853..1065
                     /locus_tag="ONT64_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304149.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1343..3142)
                     /locus_tag="ONT64_002788"
     CDS             complement(1343..3142)
                     /locus_tag="ONT64_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR2304150.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3266..3862)
                     /locus_tag="ONT64_002789"
     CDS             complement(3266..3862)
                     /locus_tag="ONT64_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR2304151.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          711 c          689 g         1187 t
ORIGIN      
        1 atattaaata ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt
       61 cataaaaaac gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag
      121 agagacaatt tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt
      181 acaaacgtct tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc
      241 cttatctaga ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt
      301 tattttcgtt ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc
      361 ggtattgcat atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta
      421 gttcttgaaa gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt
      481 tgatgcaatt aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct
      541 attttctttt aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat
      601 ataaatcata tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga
      661 acaacatagg aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata
      721 gttatctaaa aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt
      781 tcaaatacaa ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa
      841 agagggaatg aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga
      901 gtatctcatg aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat
      961 gttgaaagtt atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc
     1021 gtaaaattct gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga
     1081 attcgcaaga actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa
     1141 aaagaaaaag ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta
     1201 ttttagctat tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg
     1261 tcaaacaaaa aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac
     1321 ttttttcatt aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg
     1381 aataagtgat ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg
     1441 ttatgatacc atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg
     1501 tccatacata tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg
     1561 tactatattc cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag
     1621 gtaagatgta gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt
     1681 acgtcaaact ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa
     1741 atcgttcgac aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt
     1801 ggtttagctt atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc
     1861 cgttttcagt aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat
     1921 ttacccctgt taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg
     1981 tttcctgaac atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg
     2041 cttctaaagt attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg
     2101 gttttgtctg aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga
     2161 aaaaaactgc aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg
     2221 cttgtgggat aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa
     2281 caatgatata aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta
     2341 aaagcgtcat agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca
     2401 tactttgtgc caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc
     2461 caatcacaat aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt
     2521 cccgaatggc tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca
     2581 gtcgaatccc aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc
     2641 aacgattagc aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat
     2701 cagaattaaa acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca
     2761 aaaaagtaat gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca
     2821 aagagattgg ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga
     2881 aataaactaa cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat
     2941 gttgagaaaa aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta
     3001 aaataaaacg ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca
     3061 tcagcaagac atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta
     3121 agctacgttt caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat
     3181 gaaaaaggaa agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc
     3241 aatgactaat tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat
     3301 aaatcaaggc catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg
     3361 tatatcgttt cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag
     3421 caactggaag aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc
     3481 agccaatata tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat
     3541 aacgacgccc tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga
     3601 taaatccagc ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa
     3661 tgattggaat aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga
     3721 tggaatcacc tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa
     3781 aataaatcgt cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt
     3841 ttcgagaggc agcaatattc attattcatc aactccttag aggttctttg ttaactatac
     3901 tttactagaa aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg
     3961 taagttcatc ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt
     4021 aataacttgt tttttt
//
LOCUS       DAJSAW010000128         3987 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJSAW010000128 DAJSAW010000000
VERSION     DAJSAW010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.134"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..938)
                     /locus_tag="ONT64_002790"
     CDS             complement(<1..938)
                     /locus_tag="ONT64_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HCR2304152.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKD"
     gene            complement(1160..1522)
                     /locus_tag="ONT64_002791"
     CDS             complement(1160..1522)
                     /locus_tag="ONT64_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304153.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(1526..1864)
                     /locus_tag="ONT64_002792"
     CDS             complement(1526..1864)
                     /locus_tag="ONT64_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304154.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(1843..2262)
                     /locus_tag="ONT64_002793"
     CDS             complement(1843..2262)
                     /locus_tag="ONT64_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304155.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(2255..2488)
                     /locus_tag="ONT64_002794"
     CDS             complement(2255..2488)
                     /locus_tag="ONT64_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304156.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
     gene            complement(2491..3459)
                     /locus_tag="ONT64_002795"
     CDS             complement(2491..3459)
                     /locus_tag="ONT64_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR2304157.1"
                     /translation="MSKKRTIVNEWEKLTALSNRRLNVQTLVQTIKLNWSIDRITIVG
                     KLNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKW
                     QEGKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRADVACDLIDVPDEFITQYRII
                     DPVSFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQ
                     LRRGKATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYK
                     YRNLLKRESQNDELTNHLRETFEESADDLKKELDTWLQGLDVTEEE"
BASE COUNT         1131 a          892 c          549 g         1415 t
ORIGIN      
        1 atatcttttt ggaaaatgac cgccatctct ttttttacat tcagcatatt tttcttctct
       61 tcttcaatct tcgctctctc agaagccatc agagccttat cacgctcgtt ttcttctctt
      121 tgcctataaa tatacctcaa ctcatcagaa acgctctcac gctcttttac agccgtttgg
      181 cgcaattcag aaatttcact catgatttct ttgtattcgt gaatatcttt aattttgttg
      241 gtaccgacaa tatccctttt ccaaccgtaa tcattcatta atcgttctat agagtcaact
      301 tcttgatttc taaaattatt gaaaagcact cgaccattat ctttatcagc gtgtatccct
      361 tgttgaacca gtgctttatt aaagctcggc tgcttttgaa caccccgttt ataaccttca
      421 gcaaccggca caatattcaa gtgcaggtgc ggacttattt cgtcattatg aataacagca
      481 ttataaacag ccaaattagg gtttctctct tgaaactcct tgtaatactg aaccaaaatt
      541 ttattggcaa tcactcgatt ttgttcagct tgttgtttcg atatgccagt agaagaacca
      601 tctgctcgat ttgtctggaa atcatctaaa atgcctattt gcacaataaa ttctcgttga
      661 ggttccaact ttttatcctt gcgcactttc gctaaatagt cctcgatttt tctatcggct
      721 cttttttgtt tcgcattata tttttctacc acttcgccaa acaattcctc atatttatct
      781 gctagattcg tctgctctaa ataaatattt tcatccgatt tcgtgaagtc aatgtgctta
      841 tgccaatcat tggttttctg gcgatcagtt aactcacgat tattatgaga aatacttgta
      901 ttcttcgttc cttttttaaa tgaaatacta aaccccaagt taaaaccctc cttcgcgctg
      961 tcgctaataa aaagattgta aacctttctg ttactctttg tcaaagagta acattttcct
     1021 aattgtgaca tggatgtcac aattccaaac gctctccgag ggttttcgct gtcacaagtg
     1081 acaacgaaaa aaggagccga aagcgtatca cgctatcaac tccttttatg ctttattttg
     1141 cttcacgcaa aataatcgct tatcgtaaat tcacgtcatt aaatattgac gtaaatttac
     1201 tctcactcat ctgacttttc ttattcggta tcactccgaa taagtcgtca gattcatcgg
     1261 gataatctcg ttcgcttggc gctgattttt catcagcaag ccactcatta tccactgttt
     1321 tttcgtgttg ctgatcgtca gaaattgacg ataaccaccg ctcaaaatca gcaaaagttt
     1381 caaacccaaa acgcttgcga agagccttgc ccattgcagc attttctttc tcaatcaagc
     1441 gttttttctg ttggcgaagt ttcgcttgtt ctttttttaa tttttcttct tgcgctttaa
     1501 tatctgctaa agtcattccc ataaactaca tctccttttc tttggaatta ataacggaaa
     1561 tttggtttaa ctgcataaca tgactcatta atttcaacca aaattctttt tctgattcat
     1621 cagttgtttg cgctaaatgc ttatgaacaa attcattagt caaaactgaa gattttgcta
     1681 gatcagcatt gataaccgag ttgagaacat ccataattcg ttcaagcatt tcactataat
     1741 tatcttcatc cggcaaattc aaataatcag ctaaccgttc atacatcaat tcaattctat
     1801 cgactgtttc ttctctcaaa tcaattttat ccatgtcaca cctcacttcc agaattgcca
     1861 ccattttcga ttttttaatt cttgattttc tagtaacaac tgatcgtttt tttgttgctg
     1921 aagcaaagtc aatttttgtt ggtcacgaat catttccgtt aattcagaaa tatgcctatc
     1981 tttttccgct aattgttcac gcaaaaattc attttctttt tctaacggga aatcattgcc
     2041 tgcatcattg accggtaaat caccagtcaa ttgaccacct tttgaccgat aaattgaccg
     2101 cacaacacca atcaattcct tgtctaacat gataacccca ttatctttat accgagcaag
     2161 gtcataacct gcattgacca gttgattgcg cactgtattt ttagatatac ccaactcatc
     2221 agcaaggttt tttatggtca ttttttctgg caaatcagtc attatcttct ccgttacttt
     2281 cagctacccc ataaacatat ttaacagcat gcttagggat aatgattggg gaatcaattt
     2341 tattattttt caaaatcaac tcatactgcg ttacatcaag taacttaccg gacatttttt
     2401 tgctatccaa aaaaacaacg acaacgttat cacctgttaa attcaccaac caatttttct
     2461 taccaaaatg cttttgtttt tctgccatta ctattcctcc tctgtcacat caagtccctg
     2521 taaccaagta tccaattctt tttttaaatc atctgctgat tcttcaaatg tttctcgtaa
     2581 atgattcgtc aactcatcat tttgactttc tcgctttaat aaatttcgat atttatattt
     2641 cgagtttctt gacaaaatcg cccaattaga atgattagct aacaatccat caatcataac
     2701 tttatccgta ggttttatat cagttggtaa aaaatgtgga ctcacaaagc tatccaaact
     2761 ttctttcacc atagcatacc attccgtagc tttaccacgt cttaactgca tttctaaccg
     2821 ccaccaagta ttaatttctg aaggcaccac ctttcttttc atttcttgct ctattttttt
     2881 attgtacaaa cgaatttgcc gctcgcttgc ccgactaccc caatacaccg tttccagttt
     2941 tcctgagcgt ccataaatta atttaaatga aactggatca ataattcgat attgcgtgat
     3001 aaactcatca ggcacatcaa taagatcgca ggcaacatcc gctcgtgaaa aatggggctc
     3061 taaaaataaa tcatgaataa aatttttcat ggaatccgct aaaaatcgac tgattttatt
     3121 aggattaaaa tcaatccgcc cttttccttc ttgccattta gaaatttcaa tataggcaat
     3181 attttcgtca tgttggtcta atagctgcca accattattt ccaaccgctt ttaaatagcc
     3241 attgccttca tttacccgca taagctgttc aaaatctaat attaatacat catgttgagt
     3301 atgataaaaa atattttgat tcaactttcc aacaatggta attcggtcaa tcgaccaatt
     3361 taattttata gtttgtacca aagtttgaac atttaaccgc ctgttagaca gggctgttaa
     3421 tttttcccac tcattcacga ttgttctttt tttactcata ttcagttcct cctcaaagaa
     3481 aaaagagatt tttggaaaga atttccagag ttcacgtaac tctaaaaatt cataaccatc
     3541 aatctctagt cttgaagaaa cctaaaaata ttcgtataat gaatttgagt tacggaacta
     3601 gtgtggttag tttccgtaaa ggtcgagaac gttggtagcg ttgctcgact tatttttttg
     3661 ctctttttcc agttcatttg tttgctaact caataatagc acttctcttt agctatgcaa
     3721 ataccaaaaa aataaaaccg caccctttga aaagggcgcc cccaaacttc gctaacgcta
     3781 agttggccaa aaaatggact tgccattttt tagcaaatgt cagcttcagg ggctagtggt
     3841 ttagccgtca actccttatc ctcggcacaa accgcctccg gtaccgttga ctgcacatcg
     3901 tggctaccac tcaatctcat tctcagaacg ctctaattta tgctgtgaga cgttttgacg
     3961 ataaaataag gttttctcct gctctaa
//
LOCUS       DAJSAW010000129         3982 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJSAW010000129 DAJSAW010000000
VERSION     DAJSAW010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.135"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(55..1698)
                     /locus_tag="ONT64_002796"
     CDS             complement(55..1698)
                     /locus_tag="ONT64_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR2304158.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            complement(1695..2054)
                     /locus_tag="ONT64_002797"
     CDS             complement(1695..2054)
                     /locus_tag="ONT64_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304159.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            complement(2215..2475)
                     /locus_tag="ONT64_002798"
     CDS             complement(2215..2475)
                     /locus_tag="ONT64_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304160.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            complement(2466..2777)
                     /locus_tag="ONT64_002799"
     CDS             complement(2466..2777)
                     /locus_tag="ONT64_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR2304161.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            complement(2778..3350)
                     /locus_tag="ONT64_002800"
     CDS             complement(2778..3350)
                     /locus_tag="ONT64_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304162.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            complement(3415..3642)
                     /locus_tag="ONT64_002801"
     CDS             complement(3415..3642)
                     /locus_tag="ONT64_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304163.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            complement(3683..3943)
                     /locus_tag="ONT64_002802"
     CDS             complement(3683..3943)
                     /locus_tag="ONT64_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304164.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
BASE COUNT         1141 a          850 c          557 g         1434 t
ORIGIN      
        1 agaaaatatc tctttttcac ttctataact acccctttca ataaggagta taccttaaaa
       61 ttcaatctca tccaacatat caaatgcttc ctcataatcc atatcagtta tttcttctcg
      121 tttatataaa gctgccaaaa aagcatgaaa cccatctgtt ttacgcctta ctggctcttt
      181 tttcaaaaat gttttattcc ctaatttgtc ttctttgata aacgaattat ttgtatacca
      241 acgcatcata ggatcatcac caaaaataaa ccgttcattt gcaaaaccat cttcaataat
      301 aggagcaact tttgactgca cacctcttat atttctgata aactcaaaat tatatgattc
      361 tttctctaga agaggtttca gcaaatccat acgaaatccg tcggcacaaa cgatttctat
      421 ttgatggatt tttctttttt catttaacca atcaattaaa tgacgaggat caattgatgg
      481 ctcatcaacg attgtcacta aacctttttt ctcccaatcc ttaataggcg ctttcagttt
      541 gaatgtatct agaaactttt tcctagcaaa actatgttgc tgccagataa atttatcatc
      601 aattttaaaa agcaaaccca ctgttgcaaa atcacgaata cttgcataat caaatccagc
      661 cacacaagaa cgaccattca aatttggcaa ttcttgttta gtagccatta atttttcatg
      721 agtcgtaata tctgattctg catcaccttc tgtaaaattc attcgtttaa caacaaattc
      781 acgcctacct gatggctcat cttctagttt tttatattct ttcatcactg tagaaaacag
      841 acgttttgaa tatgctgaat cttcttcgaa cattggatta gcctttgacc acattttagg
      901 attatccatt tcatcaatgt tatccagttt acaaataaag ggaaacaagc gatcattctt
      961 gttctctcca ctaaaaatct tttgtgctcg ttccaacatt ttgtcataaa aaccttctct
     1021 aacatgacca tttgtaccat tataaaaagt tcgaggatgt tttattttcc ctagcccact
     1081 acgctgaata tcaactaggt ctgaagattc aaactgatgg atttcatcaa attctaagca
     1141 gccatctctt gcactatcca tagtcttagg attgtttgtc ctataactaa aaatcgaatt
     1201 agttacattg ttgactatag ccatcttagt caaataaaat tgtttttcca gtttgcgcat
     1261 ttgtaccatt tcataaactt ctttaaaaga tacctttccc tgcttctcag aattcgctgt
     1321 aatcgttaca tcataatttc tgacaccgta gagagggcta ataaaaaatg agtctcttgc
     1381 tgacatgaac ccatttttcc cgccgcctcg cgcaatacta tttagaattt catcaaaaaa
     1441 cacctcgtcg tcctcttttt gatagcagaa aatgaaaggt gtgataaatt tttgatattt
     1501 agctaaagga aaaaaatttt tttcagtgta cttgataaac ttttgaatca tttcttcatc
     1561 aaaatagata tcatcccgaa ctagcacttc gttttttaaa taagtaatta actgaattct
     1621 ttctttatta aaagggatta cccctttttc gtataaatct acgtattcat caaaatagaa
     1681 tggctttaat gttgtcacag caaatcacta ccatctagcg ctgcattttt tgtaggttcg
     1741 cgcttttctt ctggcaaata gtcagtaagt tgcttaatta cacgttgata tgcttggtct
     1801 ctcgaattat actgtttagc aactggtcgt tcccgctcgt aaggtttttg attttctgac
     1861 tgagagaaaa gctcataatc acctttttcg gaaatatcaa tccacatttc atcaagaaga
     1921 atttttaagc gtgccgcttg cgtaatcaaa ccagaaacga ccttcttttt atccttatca
     1981 atatccttaa acaatttatt tagacgcctt tcttcagcag aaacaagcct atttctctct
     2041 tgcagatcag ccatcaactc acttcctttc tacgtgggga gggggtcgtg tgaaaatagc
     2101 atcatttatt tgcagagttg accccatcca ccggttccca aaaattttat ttcttctcaa
     2161 aatcttttga ccgggggtat ttcactctat atatcttcgc tttatactga tttatcagcc
     2221 gtcttgctcg ttgctttcta caatatgtgc aaatggactc aaggtataat caatgtctac
     2281 attaatatta aatgtccctg ttacttcatt agcgttacct attagtctat tgttgataga
     2341 tatatcctct acagttgttg ttccttcaat gagctgactc ttaaatagaa ccaacttatc
     2401 aatagcctta tcaaccatag cgattgcttc tgctcgtttg acatcatctc tgtttgctgt
     2461 ttttcttacc accattcatc atcccacttt cttttctttt gttggccacg atatttcata
     2521 cggttgtgcc ttttgttatg gcaatctttg cataaggttc ttaggttgtt tatatctaga
     2581 gctaaatccg gatgatactc cagctccttt atatgatcca cttcaagaat tgaatcatgt
     2641 tgagttgtta gtctaccctc tgctttacac cattggcact cataatgatc tcgctctaag
     2701 cattgacttc taagtttcct ccactcagtt gatccataga acctagctct tgcttgttta
     2761 cttgaaacat caatcattta catatctctc ttgtgcccaa tgatttttct atctaagtac
     2821 ttgttattat gtgaatcata atattcaata gtgatgtcgt tagctccttt tggtacttgg
     2881 cattcatatt cttccgtatc ccaatgataa gtaatatcga caagacctat cggcatctca
     2941 tccattcgtt gacctttata ataaacttct ggtactgagt cagtatctct tagtttgatt
     3001 tctagaaggt tcgtattact tccatcatct aacgtcaatt cagcaatcct ctttttgatc
     3061 tgttgaatta cttttcttcc tgtctcacct gtcattggta ctaccgtctc atatccagta
     3121 aactcgcaag taaagcgttc aatagcataa gcgttttgaa gtggcttagc actaagaaca
     3181 gctggattat tgctataaaa acgatctact aataacttcc ctataggaat ggcatctaaa
     3241 gctctatcag taaacactaa agcattggga taatcttctt gaattttgta agctagattg
     3301 ggtgcagtta caacgttata cccttctctg atataatctt ttaatttcat agttttcctc
     3361 ctaaaaacat ctaataatta cttgctaaca acttgatgta atcatagtta tcattcattt
     3421 gatgtacctc tcaatgttgt attggatata ctcgtctttc caatagccat gaccgcaata
     3481 tatcagcttg catttatcca cttcgtttgg tgtagcttct cttagcattt cgacaataga
     3541 gtacttccct ttgatttgta cagaacgcac aacacgcact gaacaatcac caatggttcg
     3601 aggatattca ttagttagtg atacatacca gtagtttctc attatgcagc ctcctttatg
     3661 taaagaaaaa gcctagcacc tgctagactt tgatacattc atttgatttt ttgagataag
     3721 tatctacata gatttcacct ttgtccccgt tcaacgtaat ttcaaaatac ggtgctcctt
     3781 ttaagctagc gctgactaat gctttgctat tttgcagtgt tttgcatgac cagactatga
     3841 aaatactaaa ctcaggcact gctccactta ggtcagcaat ctcattttca attctttcac
     3901 gtacaatagc cttgcatttt tcgataaact tctcatgatt cattttgtac ctccgcaagc
     3961 gcataaccag ctgttgatgt at
//
LOCUS       DAJSAW010000130         3721 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJSAW010000130 DAJSAW010000000
VERSION     DAJSAW010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.136"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            75..470
                     /locus_tag="ONT64_002803"
                     /pseudo
     CDS             75..470
                     /locus_tag="ONT64_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(465..683)
                     /locus_tag="ONT64_002804"
     CDS             complement(465..683)
                     /locus_tag="ONT64_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304165.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            790..1104
                     /locus_tag="ONT64_002805"
     CDS             790..1104
                     /locus_tag="ONT64_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2304166.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="ONT64_002806"
     CDS             1208..2386
                     /locus_tag="ONT64_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2304167.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            2789..3082
                     /locus_tag="ONT64_002807"
     CDS             2789..3082
                     /locus_tag="ONT64_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HCR2304168.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1272 a          550 c          718 g         1181 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
     3421 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
     3481 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
     3541 agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct
     3601 cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat aatcccgtcc
     3661 taactctttt attcttccct catagcttcc aaatttaagc aatacttcaa ttaatcaagt
     3721 t
//
LOCUS       DAJSAW010000131         3715 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJSAW010000131 DAJSAW010000000
VERSION     DAJSAW010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.137"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ONT64_002808"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ONT64_002808"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR2304169.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="ONT64_002809"
     CDS             complement(2262..2513)
                     /locus_tag="ONT64_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HCR2304170.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="ONT64_002810"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="ONT64_002810"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCR2304171.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="ONT64_002811"
     CDS             3463..3681
                     /locus_tag="ONT64_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304172.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1026 a          797 c          688 g         1204 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caaga
//
LOCUS       DAJSAW010000132         3685 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJSAW010000132 DAJSAW010000000
VERSION     DAJSAW010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.138"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..235
                     /locus_tag="ONT64_002812"
     CDS             <1..235
                     /locus_tag="ONT64_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR2304173.1"
                     /translation="SGKSLEALELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVI
                     TNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            470..706
                     /locus_tag="ONT64_002813"
     CDS             470..706
                     /locus_tag="ONT64_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304174.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            713..2665
                     /locus_tag="ONT64_002814"
     CDS             713..2665
                     /locus_tag="ONT64_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCR2304175.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2737..3234)
                     /gene="dfrG"
                     /locus_tag="ONT64_002815"
     CDS             complement(2737..3234)
                     /gene="dfrG"
                     /locus_tag="ONT64_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCR2304176.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3464..>3685
                     /locus_tag="ONT64_002816"
     CDS             3464..>3685
                     /locus_tag="ONT64_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR2304177.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELCKIDRAYSGEIVILQNEFLKL"
BASE COUNT         1310 a          590 c          640 g         1145 t
ORIGIN      
        1 gtccggtaaa tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa
       61 ttgttctctg ttccctcttt atcatggaag tgcaaaaagt aatataggga ttgataacct
      121 tatagaagtt attactaata aattttattc atcaacacat cgaggtccgt ctgaactttg
      181 cggaaatgtt ttcaaaattg aatatacaaa aaaaagacaa cgtcttgcat attaagtagt
      241 tcagttttgg agtacaaaaa gtgaaaatgt aaacctaaat gagtatcaaa ttgcaactga
      301 atttgatact cgttttttat tgttaaatca acgtttgttg ttaatttaca ccacaaaatg
      361 taagttaaaa tgtatataaa aatgcgaacg tgaaataatt attgtaggaa tgcaaattgg
      421 tgtgttaaaa tggttacaat ttactaagca aaaaggggtg cattttccta tgcaatttct
      481 tgagccaaaa aataagaacg ctaagtctgt ggattgggaa atatcagagc aagtaagggt
      541 gatagttaag caatacgctg aatatgcaga gcgtactgaa agcgaagcag tggatgaatt
      601 cttattgaac attttagacg ataagaagtt tattgaatgg attgccaaca agcgaagtaa
      661 taagcggatt gttgaaaaaa tgggtatcaa agatagagtg ggttgataga caatgaccaa
      721 gcttaaaaga ttagccacga aggaagatga aattgtagat gttaaaattc cgatttcaaa
      781 cgaagaatta aaagataggg ctaaacaata tgatttatta actccaaaac gatttgcaac
      841 aaggtataac aaaatgttgt tcttgccaac gtcttttaaa tggaacggtt ctgaatatcc
      901 cattcaatat aattactgca tcaatccgtt ttgttgtaat tttgggaaag aacagcacaa
      961 attcaaagat gtaaaaggga aaccaagtcg atataaaatg actggtagtt ccaaagataa
     1021 aggtcataaa ggtatgtact gcaatgacaa tcctattggc agaggtgtat cgcaaaactg
     1081 tacagtaacc cctttatcta attggtctgt tgttgaagaa attaaacgcc ttatagaaat
     1141 caatagcatc caagatgttg agccagatta tcaatttcat aaagaaggct gctcggaaga
     1201 agaatcaacc ccattcaatg agccgaagca attttacaaa agggggaaaa gtaggggcaa
     1261 atctcaacgc tatcaatgta aggcgtgtaa gaagttcact aatgttctac caaaaagaga
     1321 agaaaccact acttatcacc aacaaaagaa caccattctt ccaatgttcg ctaagatggt
     1381 tgttggaagg gtttcagtca gtcgtacttg tgatatttta ggaattggag ttggaaccta
     1441 ctatcataag ctggagtggc tgtataggcg ttgtttggag tttttagaac gatatgagac
     1501 acaacccctt caaacaaaga aatttaacga gatgtggctt aatacggaca agatgcacta
     1561 ctacctaaat aacgtccgta agaaaggaca aggttcaaag aaatatacgg ggtttgaaga
     1621 ccttaatatg caaacgtata tagttgtatc tgctgaggtc ttatcaagat atgtgttccg
     1681 ttctgatgtt gcctatgatt ggaatatttc aatggatgaa ttaaatgaag atacaagaaa
     1741 attcaaagaa gaccatctca atacttttag tcgtaaaaat gataggttag attggtctta
     1801 ttatccacaa gaaccatccg ctaacgatag cgaaaatagg aatgcttatc ttcacgaatt
     1861 agggaaaatt actaatagga gtagatttgt cgatggttta aatgttgatg ctccttatac
     1921 tacaactgct cactattggt taattaaaca aatggttaat gccgacgaat ggcgaatgat
     1981 tagtgatgat gacttctcta ttagaaacgc cttttataga gtattcacga aagaattaag
     2041 gttgagcgat gcacatcatt ttatttgcca agttaacaag acgaaaagca gaaaacaatg
     2101 cttaaaagaa ttcggacaag caaaagcaga actgctcgat tggggagata tcagaggatt
     2161 caaaacaaag tttctacgga ctttggcatc tcattatcta actgaattac taacaagtca
     2221 tcaattccac gaagaggcta ttagtaaaga tggagaaaga tatcgcaaat atgccgataa
     2281 ccctatcaaa catccattag ccactaagga taaaggattt tattcagttg attgtagaac
     2341 ggatttatct gctctggaac caaacgaaat cgcaaagatg ctattaaatg ttaatgacca
     2401 ttcgaccaat agctttattc aacagataag acgttatata tcatctcttg aaagaccatt
     2461 aacaacagct cgtggtgata agaagagcta tatttacgct aacttcaatc cgaagtatgc
     2521 tcaatttgcg attacaattc taagaactta ctacaacttt tgtcgccctt ttaaatcagc
     2581 ggataaaaag gttttgacac ctgctcaaag attagggatt acagataaac aatttgattg
     2641 gaaagatata atttatttca aatgacaaaa agagcttgga gtaatcccaa gctcttgtgc
     2701 tttatcttta ctgttcttct tcaattaacg cacccgttaa ctcaataagt tttttctttc
     2761 atatacatga aaatagtagt tatacggatt tttatcattc tttatccctt tttgggcaaa
     2821 tacctcattc cattcctcat aattcacttc tggaaaaaaa gtatctcctt cgaattcatg
     2881 atgtattttt gtgatgtaca ttttctcaac ataagggaaa aacaaattat aaatctgttc
     2941 tcctccgaaa ataaaaattt cttcttcgtt tttacataac tcaaaaacat cttctattga
     3001 atgaacaatt tcacaaccat taaaggtaaa ccccttatct ctcgtcagaa taatatttct
     3061 tctgtcaggt aaggctcttc cgattgattc aaggttcttc ctacctaata ttatcggatg
     3121 tccctttgta gtatttttaa catattccca gtccttggga atcctccaag gaatgtcatt
     3181 ctctttgcca atcactctat tcttatccat cgcagcaatc aaagaaactt tcatttataa
     3241 cacagccttt ttaactaatt taccatctaa tttcttattt ctatcaaaaa accttttctc
     3301 attatcctgc ccgttagttg aataagcaat tttttctata agttatatta tcataatacc
     3361 acaaaatcac aaattttcat tcaattatca ttttcaattg cattttataa atcattttgt
     3421 atgtaatttg gtatgtaagt taaaatgaaa aaagtagtcc attttgaagt acattttgtc
     3481 attttgtact ccaaaactga actacttact tgcatatata cgcctttata gtggagtact
     3541 acatttacga gattcggtta gagtatcaga aaaagaaaaa ataaaagtta cagaaatgta
     3601 tacttcaata aatggtgaat tatgtaagat tgatagagct tattctggag aaattgttat
     3661 tttgcaaaat gagtttttga agtta
//
LOCUS       DAJSAW010000133         3534 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJSAW010000133 DAJSAW010000000
VERSION     DAJSAW010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.139"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(45..1511)
                     /locus_tag="ONT64_002817"
     CDS             complement(45..1511)
                     /locus_tag="ONT64_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCR2304178.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="ONT64_002818"
     CDS             1898..2494
                     /locus_tag="ONT64_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR2304179.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="ONT64_002819"
     CDS             complement(2552..3034)
                     /locus_tag="ONT64_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304180.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="ONT64_002820"
     CDS             complement(3140..3337)
                     /locus_tag="ONT64_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304181.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT         1199 a          707 c          578 g         1050 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaa
//
LOCUS       DAJSAW010000134         3505 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJSAW010000134 DAJSAW010000000
VERSION     DAJSAW010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.140"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            577..909
                     /locus_tag="ONT64_002821"
                     /pseudo
     CDS             577..909
                     /locus_tag="ONT64_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
     gene            923..1072
                     /locus_tag="ONT64_002822"
     CDS             923..1072
                     /locus_tag="ONT64_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304182.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1069..1515
                     /locus_tag="ONT64_002823"
     CDS             1069..1515
                     /locus_tag="ONT64_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304183.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1517..1654
                     /locus_tag="ONT64_002824"
     CDS             1517..1654
                     /locus_tag="ONT64_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCR2304184.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            1691..1984
                     /locus_tag="ONT64_002825"
     CDS             1691..1984
                     /locus_tag="ONT64_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304185.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1981..2205
                     /locus_tag="ONT64_002826"
     CDS             1981..2205
                     /locus_tag="ONT64_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCR2304186.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2202..3221
                     /locus_tag="ONT64_002827"
     CDS             2202..3221
                     /locus_tag="ONT64_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HCR2304187.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            3410..3483
                     /locus_tag="ONT64_002828"
     tRNA            3410..3483
                     /locus_tag="ONT64_002828"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3444..3446,aa:Ile,seq:cat)
BASE COUNT         1209 a          629 c          732 g          935 t
ORIGIN      
        1 ttactagatg acccttactg ggtaggtaaa acatcgttgg gtgagaatat tgcaaataaa
       61 ggtacgacgt tcccaattaa atctagcgcg tacgaaatat ataaaggtaa cacggaagaa
      121 gagcttataa taggtcaaac gtatactatc acgcttaaag gaacaaaacc cgcaagtcaa
      181 acctttgtag catataatta ttggaacgtt aattttggag aactaaaacc agttgaggga
      241 ttgacagacg tatggtctct aacattcaca ccaacgaaac ttgaaccggg tttgcctaaa
      301 gaccttcgca tttttcagtt gcctaaagaa acggccggcg catgccaaat tgactggctc
      361 aaaattgaaa aaggcgacac ccgaaccccg aatattagtc agtttaaata ctttggtgaa
      421 ggattgaaag atagcaacaa tccgaacgac tacagctggg acatcacgcc tgaatatact
      481 gaaaaaggct tgaatgatac ggttagtttg actgaaccgc aatccatcga aggattgaaa
      541 aatttcgaag atggtattca atcaaaagga aaatctgtat tgacatcaga cgacaacaaa
      601 tatgaagtcg taaccttaac agttacaaac gggaataccg gatcagcaaa gctttatcgt
      661 gaaggaaaaa ccgtcactat ttattttttt gcgttgaatg ggaaaagcag tggtggaaat
      721 gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt tgagcaactg
      781 gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg tgcagatgga
      841 gccattaaat ggcgaagaaa ctcaagttat ggatcagctt attcatttgt tatcacttat
      901 tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc cttacggatt
      961 cgagcaaatc attgtgaatg acgaagaaca tttaccggaa gaatgcacag aagtcgaacc
     1021 accgattcca aattggaaac cgaaattcaa ttactgggag ggaaataaat gaaaaacatt
     1081 tggaaatatg gccgtactgg cggagagtat gcaggaaaag tattggacga catgcttgta
     1141 tccgttcctt acacagatca gccaccgctc gaaggggttc gttcagatgg cgaaccgcta
     1201 acgattgctg atcagatgtt tgatcctaaa ttgaaccaat ggattatttt agcgaacgcg
     1261 ttagatcaca acgatttaaa caatctcaaa gcgatgtacg aggctcttga acatgaaaac
     1321 ggcaacctaa aacagctaaa tgccaaactc atgctaagcg atgtagcgat taaacaggaa
     1381 aatactgcat tgaaagaaaa agctgacagt ttagcacaaa tcaattcaaa aatgatgctt
     1441 gcttcgttac aaaatagcaa agacatttca gaaattaaag agcaactaaa tccagcttca
     1501 aaaggaggtg agtagtatgt ttagttttag cgatgtgaaa atgatgtatg attggggctg
     1561 ttttactgac gatcaagttc gactattcgt tccactatgc attacagacg aagaagcaga
     1621 taaaatcatt aataaagata agagcgcatc ttaattgatg cgtttttatt taaggtaaag
     1681 gagttgtcac atgattaatt taggggaatg gggaatgata gcaggatcaa taaccgctat
     1741 cgtttctttg attttattag taattagacc gattgctgca tctttctcga aaattactga
     1801 gactctttca aaagtaagcc acaatttaga tttgctgact aaagatttag aatcgagcaa
     1861 atcagatcga ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca
     1921 tacagaaaaa ttggtggaac acacgcaaca aatcaaaact ttgtttaggg agagaagaaa
     1981 atgaataata aaacgttcga agtactaaaa tggttcgcac tggtaattat tcccgcacta
     2041 gctactttcg tggggttagt tggtaaagcg ctcaattggc agtacacaga tatctgtgtt
     2101 gtcatcatta ctggttttgg cgcgttttta gggagtgtgt tgggtgtatc aaatcgaacc
     2161 tacaaaatgt tctcggctga aagcgaagaa ggaggaaaca aatgaaaaag aaaattacta
     2221 ttactgcgat gagcctgtta acggctcttt ttttattgcc aattaacgga tttgcctata
     2281 ctatcaacaa tgaatttaat ttgggcgcaa atgaaggtag ctcacaagta gcaaataatc
     2341 agtatatttt actgcatgaa acggctaatg aaacagcaac aggacgcaat gaagcgcagt
     2401 atatgcaacg ttcatggact agcgcttata ctgcttatat tgtgggagac ggcggaattg
     2461 tttaccaagt cggacaacct ggttatgtac agtacggtgc tggttcatat gctaatgcca
     2521 atagtcctgt gcagattgag ttacaacaca cacatgataa agcaactttt gaaaaaaact
     2581 ataaagcata cgttgaattg gctagagatt cagcaatgaa atatggtatt ccattaacat
     2641 tagacacgcc ttataaccaa ccaggaatca aatcacattt atgggtaact caaaacattt
     2701 ggggcgatca cactgatccg tatggatact tatcagaaat gggtgtaagt aaggaaaaac
     2761 tggcttacga tttagctcat ggatttacag acgaaaaccc aacgacttct gagaacaagc
     2821 ctgtcattga tccaacccga gcaggtgcag caaatccaac tttatcagat ggaacgaacc
     2881 attctcacat tgatcagttt ggagaaatcg aaaatgcgaa cttacacgtc gctggatggc
     2941 acatcgctaa ctataagtac gagtatattt tcattatgga ctacaatact gggaaagaat
     3001 tagctcgagt aagagctgat ggaatttata gaccggatgt aaatcaagct tataatactt
     3061 ctggaaatgt tggctatcat gtatctttta acatgcgtaa cttccctaat aagaaagtct
     3121 atgtcatgat gcgggcaacg aatgatccag agggaaacac taaaggcggt gcgcaagatt
     3181 tccatgacaa acgttggtat ttaaatattc ctaaacgata aaaatagctc ctcgttgagg
     3241 agcattacat aactatattg acaactataa aaatcattcg ataaaatagt gatgttatcg
     3301 catatcttca ctatcaccca taatagtcac actccaagct atgcgataac aggtttgttg
     3361 ccacacattc tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc
     3421 tcagttggtc agagcagacg gctcataacc gtccggtcgt aggttcgagt cctacagggt
     3481 acattaacgt agccatttga atcgt
//
LOCUS       DAJSAW010000135         3495 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJSAW010000135 DAJSAW010000000
VERSION     DAJSAW010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.141"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1533)
                     /locus_tag="ONT64_002829"
     CDS             complement(<1..1533)
                     /locus_tag="ONT64_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2304188.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKI"
     gene            complement(1552..1956)
                     /locus_tag="ONT64_002830"
     CDS             complement(1552..1956)
                     /locus_tag="ONT64_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HCR2304189.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(2188..2445)
                     /locus_tag="ONT64_002831"
     CDS             complement(2188..2445)
                     /locus_tag="ONT64_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR2304190.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(2570..2902)
                     /locus_tag="ONT64_002832"
     CDS             complement(2570..2902)
                     /locus_tag="ONT64_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304191.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT         1088 a          644 c          482 g         1281 t
ORIGIN      
        1 aatttttgtt aaataaatat cccctatttg aataaaacga ttgactaatt gaacttctcg
       61 actacaccaa ctattaatat cattgatgaa ggaatataat ttatcgtccg atgttaattg
      121 gatttgataa atccaagaat tcaaaaagat agacgttatt tgaagataat aatagtcaaa
      181 ttctatttga aaatctctct ctatttcctt aacatactgt ctaaatcgaa tctcttgata
      241 ggtgggatat gttaaagctg aattattttg gatatcaaca ttcttttgtc taaaatatct
      301 atcatatttc tcaaaaagct gctgcttatc agtgtctttc ttccacgttt gataaagata
      361 ctctataagt tcgttgatat taaaaacttc gtctactttt tccaaatcat ccatttgaat
      421 atattttaat tcattctgtc ttttcataac cttcatgccg ttgaaattca cccattgtgg
      481 taaatgatac atctgaatat aaagttcttc caaatcatct aagaaataaa aggcattggc
      541 acaggtcaat tgaatgatat ttttcaaatt gccaacattt tcactaaagc gatagtttaa
      601 taaaaaatct aataattgtt gagatatcag tattttcttt gctagtcttt cttcttctga
      661 tttaaaaaat atcttgatta actgttttct ttcataaaga gaacgactgt ctaatgttgg
      721 aagtgttagc tttacaggta ttcttctcaa caaagttgat aatagtttat cctcaggtgg
      781 ttctgatgtt gcaaatataa tccagcaact tgatgtgtac cagttttcat tatcacccat
      841 ccgatgaaat ttcctcgtat ccataaactg aaaaagtttc tcctgacatt cattgctcaa
      901 tgcatgaatt tcatctaaaa ataagactcc attattagct aattctgcta aaccctttct
      961 tccatcttcg gctcctgtat atgcaccttt tacatgccca aaaaaattgg acaagaaaag
     1021 ttctggatta ttggcatatt cactacaatt aactgaaatg aaagatgcct tctcttctat
     1081 ataattattg gccaacaaat ccttatacac ataatttgct aaatagcttt ttccagttcc
     1141 agtctcgcca tgaagtaaaa tgggtaagcc atttggcgga tagtagattg ctgctttaat
     1201 ttgagcaatt acatttcgca gacttccttg acttccgata accctttcta ttccaaccct
     1261 aaaagagcta tcataccatt tttccgcttc ctctgaagag ctaaaaattt gttgatttgg
     1321 aactttctcc ggtaaagaaa agaagattac tggacgagta ttgatttttc caaattctcc
     1381 atcttttacg aattcattca aatattgaga gacaatatta cgactaacat ttaatttttc
     1441 agatattttc tgtgctgtat tgttccccca tttattttta atctcagagt tctctatgac
     1501 ataattgaca atgcgttctt ttaattcttc catcacagca cctcctcgac actattcgaa
     1561 ttttgccgac agtatttttc cataaatatg acttgctaaa aaggctttgt ttagatcatt
     1621 caattggaac gttgcttgtt ttgcttgatc cgctaagacg ttgaaaagat actctggcga
     1681 aatcgcatac cccaaaatat caacgattgt tgcaatcaga tcttcttttg attcttcatc
     1741 tgaaagtaat tcacgataca acatcgtttg tttgcttact gtgtcatatt cctctaagga
     1801 ttcatctgct aaaagaacca cttgttccaa caaacggtcg gataaatatt tatagaaaat
     1861 cagccccaat aaataatttt tatattctga cgcatccatt ttactacgga gattatctgc
     1921 cgcactaaat aacttctgat ttaattctgc acccaaaaag atacactcct atctcttatt
     1981 taaagttgct atataccctc ttattttagc atactcagtt ttagaaaaag caaaaaaata
     2041 aagaaactta ttcttgataa caagctacgt gacgtatatc cctaacacaa aaatagccat
     2101 atcctttggt ttggatacgg ctatttgata ttactcattc aattatcaaa tcacagtagg
     2161 tgtgaatctc atcgggatcg gcaatatcta cttctgatta ggatcaaagg tgattttctc
     2221 aataaaggtg tgatacaaag ctttaacttg cttcttctca ctctggataa gaaagcaatc
     2281 caaactagtc aatatctgtt ggacatcttt gacttgaatc ggttcccctt gttgttgaaa
     2341 gatccctaag atttcattct ctcgaatttg taactgtctt cgtttttcag tcaattcatt
     2401 taaacgatct tttaacatcg gaaacaattc gggactatct tccaaagcag cttcctgttt
     2461 ttgctctaat agttagattt tgcgtaccat tttcttcttt tattttcctt cgattgctga
     2521 ttttaatata gtctaaagga aatttatatt tattgtattc tttataactt caagagaact
     2581 tcgtttgagt attactgtat ttttttaatt ccataagaaa attagttatt acctgccacc
     2641 taatcagcaa atctgaacct gagtcaatat aattaatatt attttccatc tccaacattg
     2701 taagtactgt tggtgttaat atagggactt tgtacaattg tatttttttg attttatcat
     2761 ctgcacaaag gagatttttt aaatctaatc cttcttttat ttcaaaagtt gaatttttta
     2821 aatctataag agtaaaacca attccttcat actgaatttc tgaaaatcga atttttctcc
     2881 cagttgatag atcaaggggc agaaaacctt ttattacatt gtgagtgtca ccatcattta
     2941 caatgtcatt aggaattttg attttagaat tctgtggagc acctattgga agataataat
     3001 tcattataat tttgtagtct tcatgaggaa ataaagtact tttaggattg ctaagatatt
     3061 ctcgtaccga atctaagaat ggcttatcca ttgaatcttc atccccattt aaactaaaaa
     3121 acattcctac aattgctttt gcaactctaa gtgaattcat tttctttatt gaatgtgtta
     3181 aatcagactt accattcttt aaacttgtat tgttataaag gaaattttta ttgtcttctg
     3241 caaatctaat atattcctca acatactcac taccaaaaaa actattacat ttactacata
     3301 acgtattgga atggcaacag tttttttgac aaattttata aggtgcagaa cttctttccg
     3361 tatgctattc cgattgtcct tgacaatgag cctcctcgga tgtgacgatc ttcggcagga
     3421 gctaacagtt aaagttagac tgcctcgcta gcgttagcac atccgagctc attatcaagg
     3481 attcgctgcg ccaat
//
LOCUS       DAJSAW010000136         3391 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJSAW010000136 DAJSAW010000000
VERSION     DAJSAW010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.142"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(22..2493)
                     /locus_tag="ONT64_002833"
     CDS             complement(22..2493)
                     /locus_tag="ONT64_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR2304192.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            2766..>3391
                     /locus_tag="ONT64_002834"
     CDS             2766..>3391
                     /locus_tag="ONT64_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2304193.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          999 a          686 c          609 g         1097 t
ORIGIN      
        1 tcttttttga gatacttttc gtcaattttc tcgtttaaat atcgatagaa attttttagc
       61 tacgcgacta aacggctttt gctctttttc atttttacgt tcaaacaaat attctaccaa
      121 tgtggtcaaa tgcctaaaaa tcgaatctgc cgagaaaaat agaatgctca acagcaaaag
      181 tgatacagct gagataccgg tgattgaaaa cagcttatgc aaaaagatga ccgaaaaaaa
      241 gttaccgcta gcacagaaaa gaatgatctg acttctgaat ttattcagcg gtgaacagat
      301 tttatagaga acgaggaacc ctactgcaat caaaagcatg gtagatgctg tagaaacatc
      361 tgttttattt agattcaaag tcaccccgca gatcaccaac gcaccgacta cgatcatatc
      421 tgttagtcca cctggcacag ctttttccat cacattcatg atgaatttcc ctctgattcg
      481 ctttttgttt tcttctagag caagcaagaa agatggaagt ccgatagtaa acaaactaat
      541 caatgtgatt tgtgatggtt ctaaaggata ggtcagagca aagatcactg aaaacagtga
      601 gagcaaaaag gagaaaatat tcttcactaa aaacaagctg gctgatcgct caatattgtt
      661 cacaacgcgc cgtccttctg caacaacttc tggcatgcgt gaaaagtcag aatcaagtaa
      721 gaccacttgt gaggcctgca tggcggcttc atttccggaa gccatcgcta tactgcagtc
      781 tgcttctttc atggctagaa tatcattcac gccatcacct gtcatagcta ctgtattgtt
      841 acgctttatc aattgaacga agattttctt ttgttctggc ttgacgcgtc caaaaactgt
      901 atatttttcg acagctgctt catattcttc ttctgataat ttagatgcat caacgtactg
      961 atcagcatgt gaaatccctg cttggacagc tacattggat acggtgagcg gattatctcc
     1021 agaaatcact ttgatggcta cttgctgttt tttgaagtaa tcaaatgtgg ctcttgcttc
     1081 ttttctgata ggattagcaa tcagaatata gcctaatggt gtgactgctt ctgtcaatac
     1141 tggttcttca agaatcccat cgtacacacc aaagaccaac acgcgatagc ctttttcagc
     1201 gaaagaaaca aactcttccg catattccgg caattgatcg cgcaaaacca tttcgggcgc
     1261 tcctaaaaca tacaccttgt ctgccaacgc tacactgctg aatttttgga cagaagaaaa
     1321 aggaataaca tggtgatagg attgatcagt ggtttttgta aaatatgctt taacagcttc
     1381 cattgttgca ttgtctcctg ccatcgcttt tgcatagtca gcaatgcatg cttccaattg
     1441 atctgactga ctgattattt cttgtttttt agagacgatc attttttgaa cagacatttt
     1501 attttctgtg atcgttcctg ttttatctac acaaaggaca tttacccttg ccaatgtctc
     1561 aatgcttttc atattgtgta aaagaactct ttgctttgct agtttagtcg cacttaatgc
     1621 taatgcaatc gtcgtcagca agtacagtcc ttcaggtatc ataccgatca atgctgcttc
     1681 cattgcaacg acactttctt tgatcgtgtt tccattaaaa aagtaacttt gagaaaaaag
     1741 caggataccg ataggaataa tgatgatccc aatccatttg atcaattgat taagagaagc
     1801 aatcatctcc gactgttctc cttctcccat tgtcttggct ttgactgtca gctgggaaat
     1861 atacgaatct gctccaactt tgtctaatcg ggcgtaagct tttccggaaa caatgaaact
     1921 gccggacatt aatggatcac ctatttcttt ggttatttca tctgcttcgc ctgtcaataa
     1981 agcctcatta acttgcagtt tcccttccac gacctctgca tctgctggaa tttgatatcc
     2041 agtctctaaa ataacaatgt catctattac aaggtcttct atatctattg tttgttctat
     2101 cccgtctcgt atgacattga cttttgctgt atttaaaaca tttagttcgt ccaagatctt
     2161 tttggatcga atttcctgaa aaatggcgat tcctgtattg gctatgataa ctggtaaaaa
     2221 agtcaagttc ttataggatt ctactaaaca aagcaaaatg gctaagataa gaaagatcaa
     2281 attgaaatac gtaaatatgt tttccaatac gatttgctga tttgttttga atgtgggatc
     2341 gactgaatta tttgctgcac ctttttctat tcgttccttg acttgatctg ccgttaatcc
     2401 ttctttatac gatggaaaaa gacggatgac tttatctgat gatctttgtg ttactttctt
     2461 ttttttactt cgatttatcc atttattctt catgtggcta cctcgattga tttcgttttc
     2521 tggatatttc caaacttctc ctatccagta tagaacagat atggaaagtg ttcctcacat
     2581 taagcttgca aacacctttc attcctgtca tatagcaaaa aaaaagggtt ctataatatt
     2641 ttgtgttggt ggaaaatcga aggcgatttt ctactaacac tttttcttcg tttcaactca
     2701 aaaagagact cacttttggt aaaatttagt cgaccaaaac gtcatcctac caaaggagtc
     2761 tctcaatgga taatcatact agaaaattac tcggattaac agataaacat ttattttttg
     2821 atgaagaatg gctgattgaa aaagaatata aaggcgttca agcgcagttt attaaaggaa
     2881 aattggatga ctcttcttcc tattgtgaac actgtcgatc ggttcgtatt atccgcaacg
     2941 gttcctatac gacacgcact cagatgttga aagtcaaaga aaagttaact attttagaat
     3001 taaaacgtac ccgttttctc tgccatgatt gtggaaggac cttttctgcg aaaacggact
     3061 tagttgatga acatcatcaa ctaacaaagg aactgaagca ggtgattctc atggaactgt
     3121 acgaaaatca atcacgtaaa ctaatcgcta agaagtactt cgtctcggat gggacggtga
     3181 cacggatctt gcgtgaagca acgaagcatt accagccacg tatgaacttc cttccgacgg
     3241 tgttgtgtat ggatgagttc aaatcaatga attctgtttc tggttccatg agttttattt
     3301 gtgttgatgg gacaaccaat cagctattta cgatccttga agatcgcaga ctttacaaac
     3361 taactcaata cttcctgcgc tttcctagaa a
//
LOCUS       DAJSAW010000137         3339 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJSAW010000137 DAJSAW010000000
VERSION     DAJSAW010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.143"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..2232)
                     /locus_tag="ONT64_002835"
     CDS             complement(<1..2232)
                     /locus_tag="ONT64_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304194.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ONT64_002836"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ONT64_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          920 a          746 c          537 g         1136 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg aatccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaat
//
LOCUS       DAJSAW010000138         3219 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJSAW010000138 DAJSAW010000000
VERSION     DAJSAW010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.144"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(436..1050)
                     /locus_tag="ONT64_002837"
     CDS             complement(436..1050)
                     /locus_tag="ONT64_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR2304195.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1656..2033)
                     /locus_tag="ONT64_002838"
     CDS             complement(1656..2033)
                     /locus_tag="ONT64_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304196.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2333..3214)
                     /gene="galU"
                     /locus_tag="ONT64_002839"
     CDS             complement(2333..3214)
                     /gene="galU"
                     /locus_tag="ONT64_002839"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR2304197.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          978 a          660 c          499 g         1082 t
ORIGIN      
        1 gtgtatttta tttctattag aaaaaggtat cctgttggac aattttgtta tatcaggtaa
       61 acttcattgc aataatgata acaaatgtac tataattact ttgtaaagag ttttggggaa
      121 gactctttca tcaacgtgta ttaagagaca agaaagaatc catatggatt ctttcttgtc
      181 tctttttcca ctacttttta tttttctgac actacatatt cttataactc atttctcata
      241 actgtgaaac aacttataaa aataacaaac tttaaacaaa acaagcttaa aatcaaagat
      301 gatccatctt aaaataacta aaacaaaaaa agtagtcgtt gttaatcctc attaataatc
      361 gttttctttt tttcaaacgc tccattttta tgtggaacat tattttttta aggaactttg
      421 tcaacatgtg gataactatt ttccacttgt gtcctggact agctgttttg ctgtaaaata
      481 caaaacttgc tgcagcaaaa agatcgttgc aagagaggct gttccgcaga tcgctggatc
      541 tagatttggc aaagtcaaga ctgcatcatg taaattggcg tggttgtcca aaaccaaatc
      601 agcaaattca aataatcgca aaccactgct atgttgcgat ttgatacttc gtgacaaatc
      661 aaatccagta acaatgatta atggatgacc attttctttg atccactgag ctagttcgat
      721 gattgccggg tttcttcctt cattcgaaag aagaagaaaa atgtcttctg atttgacaga
      781 aagctgatca ataattgcct gtcctgcacc ttctagcgtt tcatagacac ctccggctgg
      841 attttcaatc aatatacttg gtacaagatc cggggtttga tcccaaaatt caaatgctac
      901 accacttagt gtgcggctag catatatctg caatctacca ccttgattaa tttgtttcgc
      961 gatccaagtg ccagcttgaa gcatcagcgg ctcttctatc cttaatttct cttccaattg
     1021 gtgataatag tcaaaataac ataaactcat ttatctctcc cgcctagcta tacttgacat
     1081 gaatagaaac agctgcctaa tctaaggaag tacataaaaa aacagtagtt agacagtaac
     1141 acatgtggtc tttgctgttt caaacacaaa aaaggcattg aacgaatacg cccaatacct
     1201 tgacatagca aataaaacaa cgtgcagacc atggaaaata tctacatagg ggtagaattt
     1261 tcaccctaga ttaggtacaa taaattaggt cgatgcactg cattgactat ggcaaaatga
     1321 tcggtcaggg tgtatgtgtg tgactggtca ttccctgatt cttagcgcca actaagggtc
     1381 agggagcctt tttttattca gctgtttcta tctgaattta ttgtaagtaa tctcttatcc
     1441 aagtgcaagc aagaaaatac aaaaaaacat tggtatgatt tatatatata taaataacat
     1501 aaaaaaagga atggcttatc ccgatacaac aagcattcct ttaaaaaaga ttcttttata
     1561 taagtgaaaa aagctttttt ccaccaatta aataaatctc tcatctcaat agtttatcca
     1621 ttattttatc tttttttgcg tatacctact aaatattacc gtcttcttct cgttcttcta
     1681 ctttgttcga gagaatctgt actagaaaaa gtagttgtat gtctaccttt tctgcctatc
     1741 cgagactttc ttttcatata tgggatataa atgattagat acagtacagc aaaaaataaa
     1801 gcaaatacgc aagcttccca actacctaac gccaattcaa ccatcaaggt agcaacccca
     1861 acggatgctg cataccagat aaaaatcaga aacgtttctt tttttctgac catgcgaaac
     1921 atgataaata gactaataag caatagtggc aacacaaata tccaattgct tccttccatc
     1981 agccacttga gtatatcaag cagcgactgt gcaacagcga ctaattttcc catagtgaac
     2041 acacaccttt ttttataatt aaaaaattcg cctttttatt ctactatgaa tcttctggct
     2101 tggctttcat ttctcttatg taatcatttt cttttataca acttaaaaaa aaattaaacc
     2161 attagcataa tattttctga atctattcac gaaagtcttt ttctatcttt cgttttccac
     2221 gactcaactg atctagagtc aaaaaactaa aacgacagtt gcgcaaatga cgcacaagct
     2281 gccgctttaa taacaaatag cctttcattc tcagcaggcc acttatttcc agttaacttt
     2341 ctgttcttag ctttttactc aactggataa tatatgcact taatgaagtc ccaatttccg
     2401 aatgttgaag tccatattca atatttgtct tcatatatcc taacttatcg cctacgtcgt
     2461 atctttttcc tttaaactct ctcgcataga ccgcctctgt ctggttaaga gtctgaagtg
     2521 catccgttag ctgtatctcg tttcctgctc ctggttcttg agtttctaat atctcaaata
     2581 ttttaggagt cagtagataa cgtccgataa tggctaaatt acttggtgct tttcccattt
     2641 caggtttttc tacaaaccct tttactctgc aacgaccctt ttcgctatct gttttaggat
     2701 caataattcc gtatttagat gtttcatttt ctggtacacg catgacggca acagcggaag
     2761 cttgtgtttc ttcataatcc atcatcaatt gttgagtcaa tgggatatca tctttcatca
     2821 aatcatcccc cagcatgacg acaaacggtt catttcctat aaatgctttt gcttgaagca
     2881 cggcatcccc cagtcctctt ggatgagatt gacggatgaa gtgaagattg acttgagtcg
     2941 tttcttcaac taaagcgagt aattctgttt tttccttttc ccgcaaattg ttttccaatt
     3001 cgatatttgc atcaaagtga tcttcaatgg gtcttttttc ttttcccgtt acgatcaaga
     3061 tgtcttcgat tcccgaatcc aatgcttctt ccacaataaa ctggatcgtt ggtttatcca
     3121 cgattggaag catttctttt gccatggctt ttgttgccgg caaaaaacgt gttcctagcc
     3181 cagcggcagg aatgactgct tttttgacct tcacttgct
//
LOCUS       DAJSAW010000139         3194 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJSAW010000139 DAJSAW010000000
VERSION     DAJSAW010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.145"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            12..3161
                     /locus_tag="ONT64_002840"
     CDS             12..3161
                     /locus_tag="ONT64_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCR2304198.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1124 a          543 c          685 g          842 t
ORIGIN      
        1 agggaagaag aatgaaaatt agtcaccgtg aggaagcgga agtcgaagaa cagttgattc
       61 gtgtcctcgg ggaaggacat aatcagtgga cgtatcgtcc ggatctaaaa tcagaagaag
      121 atctttggag caatttacgt caaaaaatca tctcaaataa tcaagcagaa ctgaatgatt
      181 ctcctttgac cgataaagag tttgaaacga tcaaaaccga attattgcta cggacgaaaa
      241 ccccatttga tgctgcgaaa tggttaaaag gtgaaaatgg gatggctcgg atcacgattg
      301 aacgggaaaa tccgcaattg ggttctgtct cacttatcct ttattccaat caagacattg
      361 gtggaggaat ctctacttat gaagtggtcc atcaaattgc aaaacgtgga agtaacatag
      421 aagcgcggga tcgacgtttt gacgtcacct tattgatcaa tggcttaccg attatccaaa
      481 tcgagctaaa acaagtaact gcaaaagatg gtttttatca ggcgtttaat caaatcaaaa
      541 aatatgctga agaaggcatg tttcggaaca atatcttttc gaccttacag ttgtttgtgg
      601 tttccaatga acaaacgact cggtattttg caaatgcctt accgaaggat ttgcatccta
      661 agtttttatt tagttggcga acgaaagata ataaaaaggt agaaaactta tatgagtttt
      721 gcaaacaagt gctaaatatc ccagatgccc atcgcttgat tgcggattat acgattgtca
      781 gtgaagatca agacaataaa accctcatgg tgttacatcc ttatcaagtg catgcgattc
      841 aagccttgtt tattgccgca aataaacatc aatcagggta tgtgtggcat gcgactggtt
      901 caggaaagac gttgactagt tttgtatcta ctaagctttt agcacgaaaa tcggggatcg
      961 atcgaacgat catgttagtg gaccgcaaag atttagataa tcagaccacc acagagttta
     1021 cgaaatttgc ttcggagttt aatactgggg tttcttctgg taatgccaaa gcgaatagct
     1081 tgatcgtggg aacaggaagt gccaaagagt taagtgaaac tctgttggca gatgccaatg
     1141 cgaatgttgt gatcattaca acccgtcaaa aactagatgc ggcgttgaaa tatgcaaaga
     1201 aacaggaaga gaaaaaagga accaatcgct tccaaaaatt aatggggcaa catatcgtgt
     1261 ttgtagttga tgagtgtcat cgagcgttaa gtgctgaaaa tatggaagaa atcaaaaaaa
     1321 tgtttccgaa atccacttgg tttggcttta caggtacacc gattttccct gagaatcgga
     1381 aacaagccaa agggcaattg gcacgtacga cccatgatca gtatggagag gtcttgcaca
     1441 cctataccat caagaatgca ttagaagatg gctcggtttt aggatttcaa gtagagcatg
     1501 aaaacaccgt ggagccgact tcattagaga ataaaatcta tcggaaatta aaagaagtag
     1561 aaacatatgc agagtattca ccggaacaga tcaatcgtat gatcgatcaa atggagtctg
     1621 tcaaaaaaga aagttatttg gaccctactg tttttgaagc agaggaacat atccaaaaag
     1681 tcattcataa aatgttccgt ccagacaatg cctatacaaa atttgatttt cgaaatggcc
     1741 gtccaacgaa gtctgcgatc ttaaccacga gttccattga tatggccaaa aagtattatc
     1801 gggccatcaa agagatgacg aaagaatctg attggttaat aaaagagttt cccaatcagc
     1861 ctatccgtga aggacgaacg atggaggatc ctgatttccc acgaatcgct attacctatt
     1921 cattagaaga aaatagccga gattcaagtg cccaacaaga agaaatgcaa aaaattattg
     1981 aggaatataa tcagtattat gatactgcct ggtcactagc agacattgag cgttataacg
     2041 gagacatcaa taatcgtttg gcacgaaaaa gagcggaatt caaacaattt gggaaacaaa
     2101 tcgatttagt aatcgtagtg gatcgtctat tgacaggttt tgacgcccct acgattcaaa
     2161 ccctatttgt ggatcgtaat ctagagtatg caggattgat ccaagccttt tcacgtacga
     2221 accgtacgta tcctgaaaaa acaaaaggac taattgttac tttccgaaag ccagcgacaa
     2281 tggagaagaa tgtcgaggat gcgacaaaac tgtattcaga agctaaagaa gaatcgggtt
     2341 tgatttatcc aagttaccaa gaatccaaaa aaagattcaa gcaagcatat ggtaagttaa
     2401 aagaggtttc atccatggaa tccattgatg aacatacgcc acttgaaatc cgtatcgagt
     2461 acgtcaaagc atttcaagaa ttgaatcgtg cgtatgaatc gttagtgact tatgatgatt
     2521 acaatgatga catggtgcaa tcttcaactt tgaacaatca aattcaactt ttggaagaac
     2581 aaattggcgt gtacgagacc gtgaagggtt cattgatgga ggaagaacca gaaaaagagg
     2641 gagaagattt ttcaacgatc gaattttata gtgaaaatac cactaagttg tatgatattg
     2701 attctactta tattgatcaa ttactaggaa cttatgctgc gaatagttca gatgcacgag
     2761 aagaaattga aaaagctttg gctaaattga ataaaacaga aggagtcaaa gaagtttacc
     2821 gacaaatttt aaatgcattt gatgtgggga ctttagatag taatgaagat atctttgtca
     2881 ttaaacgtcg ctattttaca caagcaagtg ataatttgat tcatgccttt tccaatgagt
     2941 ggtttgtttc tgagaaagaa ttacatgctt ctaatctgca atatatgcca ggtgaagatc
     3001 caattcctaa tatgaaagca attatcaaca gtaaggatta cgaaggatat aaagccaaac
     3061 atccagaagc gaaaccattt aaatacccac aagaaatgaa gcgagcatgg cgtaaaatgt
     3121 tagatgatga gttgattcca cttgaaaatg aattgaggta aacgagaaga gttaagaaaa
     3181 ttgtacgatt caaa
//
LOCUS       DAJSAW010000140         3128 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJSAW010000140 DAJSAW010000000
VERSION     DAJSAW010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.146"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            103..1347
                     /locus_tag="ONT64_002841"
                     /pseudo
     CDS             103..1347
                     /locus_tag="ONT64_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="ONT64_002842"
     CDS             1360..1992
                     /locus_tag="ONT64_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR2304199.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="ONT64_002843"
     CDS             2197..2424
                     /locus_tag="ONT64_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCR2304200.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="ONT64_002844"
     CDS             2493..2759
                     /locus_tag="ONT64_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR2304201.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="ONT64_002845"
     CDS             2749..3018
                     /locus_tag="ONT64_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR2304202.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DAJSAW010000141         3115 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJSAW010000141 DAJSAW010000000
VERSION     DAJSAW010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.147"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(1..940)
                     /locus_tag="ONT64_002846"
                     /pseudo
     CDS             complement(1..940)
                     /locus_tag="ONT64_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(1161..1523)
                     /locus_tag="ONT64_002847"
     CDS             complement(1161..1523)
                     /locus_tag="ONT64_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304203.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(1527..1865)
                     /locus_tag="ONT64_002848"
     CDS             complement(1527..1865)
                     /locus_tag="ONT64_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304204.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(1844..2263)
                     /locus_tag="ONT64_002849"
     CDS             complement(1844..2263)
                     /locus_tag="ONT64_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304205.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(2256..2489)
                     /locus_tag="ONT64_002850"
     CDS             complement(2256..2489)
                     /locus_tag="ONT64_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304206.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(2486..>3115)
                     /locus_tag="ONT64_002851"
     CDS             complement(2486..>3115)
                     /locus_tag="ONT64_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR2304207.1"
                     /translation="ISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRV
                     VDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWRLEL
                     QLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKNTKK
                     KYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT          868 a          701 c          433 g         1113 t
ORIGIN      
        1 atatcttttt ggaaaatgac cgccatctct ttttttacat tcagcatatt tttcttctct
       61 tcttcaatct tcactctctc agaagccacc agagcctttt ctcgctcatt ttcttctctt
      121 tgcctagaaa aatatacctc aactcatcag aaacgctctc acgctctttt acggccgttt
      181 gacgcaattc tgaaatctcg ctcatgattt ctttatattc gtgaacatct ttgattttgt
      241 tagtaccgac taactcacgc tcccaaccaa aatctgccat caaacgttca attgactcaa
      301 cttcctgatt cctaaaatta ttccaaatca ctcgaccatt ctctttatct gctcgtaaac
      361 cctgctgttg caaggctttg ttaaaacttg gttgcttttg aacaccccgt ttataccctt
      421 cagcaacagg aacaatattc aaatgcaggt gcggacttac ttcgtcatta tgaataacag
      481 cattgtagac ggataaatta ggattccgtt ctttaaattc tttgaaatat tcaactaaaa
      541 ttttgttggc aatcactcga ttttgttctg cttgttgttc cgatatgcca gtagaagaac
      601 catcatcacg agtagtacga aaatcatcaa gagtaccaat ttgcacgata aattctcgct
      661 gtggttccaa ttttttatcc ttgcgaacct ttgctaaata gtcctcgatt tttctatcgg
      721 ctcttttctg ctttgcatta tatttttcta cggcttcacc gaataattct tcatattttt
      781 cacgaatatt tgtttgttct aaatagatat tttcatccga ttttgtgaaa tcaatatgct
      841 tatgccagtc attgattttt tggctatcag ttaactcacg attattatga gaaatgcttg
      901 tattctttgt tccctttttg aacgaaatac tgaaccccaa gttaaaaccc tccttcggct
      961 gtcgctaata aaaagagtgt aaacctttct gttactcttt gtcaaagagt aacattttcc
     1021 taattgtgac atggctgtca caattccaaa tgctctccga gggtattcgc tgtcacaagt
     1081 gacaacgaaa aaaggagccg aaagcgtgtc acgctctcaa ctccttttac gctttatttt
     1141 gcttcacgca aaataatcgc ttatcgtaaa ttcacgtcat taaatattga cgtaaattta
     1201 ctctcactca tctgactttt cttattcggt atcactccga ataagccgtc agattcatcg
     1261 ggataatctc gttcgcttgg cgctgatttt tcatcagcaa gccactcatt atccactgat
     1321 ttttcgtgtt gctgatcgtc agaaatcgac gatagccacc gctcaaaatc agcaaaagtt
     1381 tcaaacccaa aacgcttccg aagagccttg cccattgcag cattttcttt ctcaatcaag
     1441 cgttttttct gttggcgaag tttcgcttgt tcttttttta atttttcttc ttgcgcttta
     1501 atatctgcta aagtcattcc cataaactac atctcctttt ctttggaatt aataacggaa
     1561 atttggttta actgcataac atgactcatt aatttcaacc aaaattcttt ttctgattca
     1621 tcagttgttt gcgctaaatg cttatgaaca aattcattag tcaaaactga agattttgct
     1681 aaatcagcat tgataaccga gttgagaaca tccataattc gttcaagcat ttcactataa
     1741 ttatcttcat ccggcaaatt caaataatca gctaaccgtt catacatcaa ttcaattcta
     1801 tcgactgttt cttctctcaa atcaatttta tccatgtcac acctcacttc cagaattgcc
     1861 accattttcg attttttaat tcttgatttt ctagtaacaa ctgatcgttt ttttgttgct
     1921 gaagcagggt caatttttgt tgatcacgaa tcatttctgt taattcagaa atatgtttgt
     1981 ccttctcagc taattgatca cgcaaaaact cattctcttt ttctaaagga aaatcactgc
     2041 ctgtgttatt gaccggtaat tgaccagtca attgatcact ttttgaccga taaattgacc
     2101 gaacaacacc aatcaactct ttatctagca tgataatgcc attttcttta tactgagcaa
     2161 ggtcaaaccc tgaattgacc agttgattgc gcactgtatt tttcgataca cccaattcgt
     2221 cagcaagatt ttttatggtc atcttttctg acaaatcagt cattagcttc tccactactt
     2281 tcagctaccg cataaatata tttaacggcg tgcttaggga taataatcgg agagtcaatt
     2341 ttattatttt tcaaaatcaa ttcatattgc gttacatcaa gtaacttacc ggacattttt
     2401 ttgctatcca aaaaaacaac cacaacgtaa tcacctgtta aatttaccaa ccaatttttc
     2461 ttaccaaaat acttttgttt ttctgtcatt tttcttcctc ctctgttacg tctagcccta
     2521 gaagccatgt gtctaattct ttttttaaat cgtctgccga ttccgcaaag gtttctcgca
     2581 aatgattcgt caattcatca ttttgacttt cttgcttcaa caaatccctg tattttttct
     2641 ttgtattctt agctagattt ccccactcag aatgatcata aagcaaacct ttcaccataa
     2701 tccgttcagt aactttagta tttggcggaa aataatgtgg actagcaaag ctatccaaac
     2761 tttcatgaac catctcatgc caatcagttg cttttcccct tcgtaactgc aattctagtc
     2821 gccaccatgt cgttatttcc ggcggaacaa ttttacgttt tctttcttgt tccagttttt
     2881 tattatacat ccgaatttgt cgttcactgg cacgactacc ccaatatgcc gtttctaact
     2941 taccagaacg cccataaatt ggtttaaaag acacaggatc aactacccga tattgtgtaa
     3001 taaactcatc aggtacatcg ataatgtcgc aagcaatatc cgcacgtgaa aagtgaggtt
     3061 cgataaacaa atcatgaata aaatttttca tggaactagc taagaattga ctaat
//
LOCUS       DAJSAW010000142         3087 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJSAW010000142 DAJSAW010000000
VERSION     DAJSAW010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.148"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(25..162)
                     /locus_tag="ONT64_002852"
     CDS             complement(25..162)
                     /locus_tag="ONT64_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCR2304208.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="ONT64_002853"
     CDS             complement(159..1343)
                     /locus_tag="ONT64_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR2304209.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1602..1892)
                     /locus_tag="ONT64_002854"
     CDS             complement(1602..1892)
                     /locus_tag="ONT64_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304210.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(1882..3024)
                     /gene="dcm"
                     /locus_tag="ONT64_002855"
                     /pseudo
     CDS             complement(1882..3024)
                     /gene="dcm"
                     /locus_tag="ONT64_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
BASE COUNT          842 a          739 c          540 g          966 t
ORIGIN      
        1 aaattttgtc cgaaattcat cttgtcactt tcctttcttt tccattgctc gatccgctgc
       61 tgcacttgca ttcatcaagc acatcacagt aacaccaagc atactgccac caaataccaa
      121 aaaaatatct tttacaattc ccatgacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatcttttta tgtcggtctg ataagcgtgc attctgtacc
      241 atatcctgga tataggttgt ccgattcaat cgatccaact ccttagccat tttccacgta
      301 ggggcgactt gtcggcctag ccaattcaag gttcgttcaa tggtatacgg ttttggctcg
      361 gaagttaatt gaatctcttt ccgattccgt ccaataaaac gcccccattg ttcattcaag
      421 ggccagttgg ttcgcctttt accttctact ttatccgcaa atcgcaagta acgattgata
      481 atggaaaacg tggtgctctc aatgctacga ccttttaaca aatcttgaat cgcatgaaaa
      541 gcacggtcat ttttcagtcg aatctcaaaa cgatttttcg tctcagtttg gtctaatgga
      601 atccctaact tgatatattg ttcatagtct ttttcataca aacagaaata aacctcactc
      661 ttgagactgc cgatatataa ggtattgccc atgccgtcct tttcatcagc ctttaaaagt
      721 tccccggaac gataactttt aaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta gctcaggtat atccaatagc cccactcgat cattgattgc caagtccaag
      841 cgtttcatca cgccaccggc ctttagacaa tcctcgaaaa agtcgtacca gctacgcttt
      901 tgagcgagta aaaaagtttc aaattgtcgg caacctcgcc cctttaattc taaaagcaca
      961 cctttatctt cttcatgaga caacatcacc acaatatcac ccatgacata ctgttcttga
     1021 taggagtaaa aggcataatc ttcatggagc atatactcaa atttcaaatg gagaatttct
     1081 tgaataattt ttcgtgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gtcaaaggca aatcgggatt aaagctttcc agttggtcaa aaattttcct catggttttt
     1201 atagatgcgg ttacgccacc attttctatc tgatttaagt acggccgact aagtccacaa
     1261 gccgtagcca atttattttg tgaaacgccg tattcgttgc gtcggtcttt taatagccga
     1321 taatctaaat tccgttgtgc caagttctca cctccaaatt caaggtgtta actttttcaa
     1381 atgagttaac acctatacaa attacttact atctttgata caacaagcat tcttaatggt
     1441 tgcttactta ttttttgtgt ttttgtaccc ccctgttaga tacgggggtg agacatcatg
     1501 gtcggctaac gccgtccatg aacaggcaca gcaccacgga ggatttctgc ttcgcgaatc
     1561 ctccgcggtc agcgcctaac agtcagttgt gttggtttag gttactcatc tgtaatacca
     1621 tttacttcca actctgcttt catttctaac aagtattgac gaatatctgg ttcttttgct
     1681 tggtccgcaa tccgctcaaa ttctgcaatt acttgattta tttcaattcg agcatccgtc
     1741 tcctcattca ttaaatatgg atcgccagcg actttgacac cattcttgcg aatatcaaat
     1801 tttaaccgtc gcaattgttc ttctaattct gggtctgact gaatctttcc aatcacccaa
     1861 taaatttctc caatcaactc atcataaacc atgtacatcc aactctttta atcttctggc
     1921 aatatcaaaa atgaccggaa ctgttacaga gtttccggct tgcttataca actgactatc
     1981 tgaattgact tgagaatctc gatcaaaagc ccaatcggga aagccctgaa gtcgccaaca
     2041 ctctctaggt gttagcttcc gaatttgata ctgatccgtg acgatcgctt gttggtctcc
     2101 tgttagtaag gtatgaacac tctgttttcc cagacgacct cggcgacttg tggaattagg
     2161 aaaagccaga ttcacactgt cttctggctt cacctcttga tattctgttt cattttttcg
     2221 aatcatgatt tttgtaccac cttgtttcgt tgacaaggtg ggggcggttc cagtaacttc
     2281 ataaactcga ttaccttgtt caaactttcc cggtaatagt cctgctacag agattccatc
     2341 ccttgctggc agtgcgattt tgattccttc atcacctgtt aaggttggcg ctacgccatc
     2401 tgaaccatag acttgtccac ccattcctcg attggaagga ttgatattta ccaaatttct
     2461 gatgttttga tcagatgttg aagacggtcc aatgatagga aatacttgtt cggtacatgc
     2521 gtctcgagaa tgtccgataa ggaatatccg ctcccggctt tggggaatgt aggcctttga
     2581 gttaagcacc taccactcca cgtcataccc caatccatcc agcgttcgga ggatcgtctc
     2641 gaacgtagcc cctccttcgt gattgagcaa tcctcggacg ttctcaagga ataaaagctt
     2701 aggtcggaga agagtggtga accgagcgat ttcaaagaat aaagtacctc gagtatcgga
     2761 aaatctttgc cgctttcccg caattgaaaa agcttggcac ggaaatccgc cgcaaaggat
     2821 gtccactggt ccgagggatt gaataaactc atctgataca cttgtgatgt catgcatttc
     2881 cacctcctta cttgtatcat gaattgcttt ataactacgt ctagcaaatt tatcaatttc
     2941 acaaaaaccg atacaatgat ggctcgcctg ttccatacct aaacgaaagc caccaatacc
     3001 ggcgaataaa tctaaaaatc tcatacatta cctcattcat tgcaaagaaa aaagccaaga
     3061 acattttgct cttggctcat acattat
//
LOCUS       DAJSAW010000143         2970 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJSAW010000143 DAJSAW010000000
VERSION     DAJSAW010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.149"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1372
                     /locus_tag="ONT64_002856"
     CDS             <1..1372
                     /locus_tag="ONT64_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2304211.1"
                     /translation="VDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTY
                     PDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVT
                     DVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTD
                     LNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVT
                     VTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNK
                     KGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLP
                     DGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNG
                     SKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKN
                     RKKENE"
     gene            1506..2849
                     /locus_tag="ONT64_002857"
     CDS             1506..2849
                     /locus_tag="ONT64_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR2304212.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         1180 a          400 c          565 g          825 t
ORIGIN      
        1 agtagatcta acagataatg ttactaactt gccagcgtta ccagaaggaa caacagtaac
       61 agatgttact cccggcggta caattgatac caacacacca ggtaattacg aaggtgtcat
      121 tgaagtaacg tatccagacg gtacgaaaga tacggtgaaa gttccagtag aagtgacaga
      181 caatcgttct gacgccgata aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg
      241 aggcacagta gatttaacag ataatgttac taacttgcca gcgttaccag aaggaacaac
      301 agtaacagat gttactcccg gcggtacaat tgataccaac acaccaggca attatgaagg
      361 tgtcattgaa gtaacgtatc cagacggtac gaaagatacg gtgaaagttc cagtagaagt
      421 gacagacaat cgttctaatg cagataaata caatcctgaa ggacaaaaag tgactacaga
      481 cttaaataaa gagccagatg catctgaggg aattaagaac aaagaagatt taccaaaagg
      541 aactacttat gcttggaaag agaaagtaga tgttagcaca gctggaaata aaaaaggtac
      601 tattgtagtg acatatccag atggatcatc tgatgaagtt gaagtagacg ttacagtgac
      661 agatactcgt tccgatgcag ataaatataa tcctgaagga caaaaagtga ctacagactt
      721 aaataaagag ccagatgcat ctgagggaat taagaacaaa gaagatctac caaaaggaac
      781 tacttatact tggaaagaaa aagtagatgt tagtacagct ggaaacaaaa aaggtactgt
      841 agtagtaaca tatccagatg gatctaaaga agaagtagaa gttactattt ctgtagtaga
      901 taaaaaagca ccgaataaac ctcaagttga tcctattaca gagggtgacc aaattgttac
      961 tggtaaaact gaaccaaatg cagaggtgac agttacatta ccagacggaa gtcaacacca
     1021 tgggacagct gataaaaatg gtaattttac agttaaagtt cctaaattag aggcagggac
     1081 aaaagtgata gtaactgcaa ctgatgaatc tggtaatact agtgaaccta ctaatgtagt
     1141 tgtttcttcc aacaaaaaag acagtggaaa aaacggtagt aaaggtagta agactgacaa
     1201 tcaaggtagc aacagcaatc aagacaaaaa tagaggcaaa agccagtcct caaaagtttt
     1261 tcctaagacg ggagagagtg acagtaatat ctttactatt agcggtggtt taatactgtt
     1321 aggaacgtta ggattattag ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa
     1381 tttcttaagt cttctctgag aagaggaaga atctttttct tcctcttttt gcttttttat
     1441 gatgggaaat aagtgttagt taaagatgaa tatacttttt atagaaagga aaagaatgta
     1501 attaagtgga aaaaatagta attattggtt caaaccattc agggctagcc gcagctcgat
     1561 ttctaaactt aagcgattca aattatgaag ttattctaat cgataaaaaa tcagaaatta
     1621 gttacttgga atgtgggaca tcgttattta taaaaggaga agtaagtaat ttagaagatc
     1681 ttttttatca aaatagaatg acactaaatt ctgagataaa aaagttattt ctagagacag
     1741 aagttgacaa aattgatatt caaaataaaa agattagttg tgtaaaaaaa gatggtacaa
     1801 gattctctca agaatatgac aaagttatat tagctacagg ttcaaagcaa attatgctag
     1861 atataccggg gaataattta gctaatattt tttcgataaa gaattttcca aatgcaaaag
     1921 agatttttaa agctttggat gatagtacta ttaaaaaagt aggaattatt ggagcaggat
     1981 atattggagt tgaaattgct gaagccataa gaaaaagagg gaaagaagta tatcttttcg
     2041 atgttgcaga tagagtgttg tcgacttatt acgaccgtag tttttcagat aaagttgaag
     2101 aaatactatc aaaaaatgga atacatcttt gtctatcaga aagtcctaaa aaatatttag
     2161 gaagaaaaaa gattgaaaaa atagtcacta caagtggtac ccaatactct atggatatgg
     2221 tcgtacaagc tataggattt ctatctaata gtccaattgg ggaaaaggaa ctcttgagag
     2281 atgttagtgg agcatatcta actaatgaat accagcaaac aaatataaaa gatatatatg
     2341 ctattgggga ttgtgctaca acttttttta cgtccattaa tagaagtagt gttgattttt
     2401 ctatttctaa tgctctaaga acagcttata ttgcaactca acatattaac aatcaagatt
     2461 ttacaccttc tggttcccaa tttacaaatg gcttttctat atttaattac aatttttacg
     2521 cagcaggttt gaatttaaaa acagcacgat tattaagtga gtctatagat tatatagaaa
     2581 tagaagattt cattagacct gcttttttga gaaaaaatag tcgtgtaaaa ttaagaatta
     2641 gctttgaaaa aaatacaaag agaatagtag gtgtacaact ttgtagtcaa gaagatttat
     2701 ctggactatt atcaatgttt tctcttgcta tagaagaaca aattacatta gacaaattga
     2761 aaatgttaga ttatttattt catcctagtt ttagccaacc ctataatttt attttaaagg
     2821 cagtaacagg attaaacatt gaaacataat cgttactaaa tgaaatcaag aaatatactg
     2881 tattactcaa ttcattgaaa atatttatca taatttttaa tagaaaacac tattaaagag
     2941 aaaaaagaga ggtaaatcaa atgaaaaaat
//
LOCUS       DAJSAW010000144         2908 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJSAW010000144 DAJSAW010000000
VERSION     DAJSAW010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.150"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            62..676
                     /locus_tag="ONT64_002858"
     CDS             62..676
                     /locus_tag="ONT64_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2304213.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(841..1665)
                     /locus_tag="ONT64_002859"
     CDS             complement(841..1665)
                     /locus_tag="ONT64_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304214.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(1689..1844)
                     /locus_tag="ONT64_002860"
     CDS             complement(1689..1844)
                     /locus_tag="ONT64_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304215.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
     gene            complement(2303..2560)
                     /locus_tag="ONT64_002861"
     CDS             complement(2303..2560)
                     /locus_tag="ONT64_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR2304216.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(2553..2822)
                     /locus_tag="ONT64_002862"
     CDS             complement(2553..2822)
                     /locus_tag="ONT64_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR2304217.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT          958 a          506 c          388 g         1056 t
ORIGIN      
        1 atggcttcct cgtgtatgat tagggtgtat tttaaatagt tgcagaaagt aggtaaaagt
       61 tttgagagtt gcatacatac gtgtctcatc gatcgatcaa aatgaacaac gccagattga
      121 agaaatgaaa aaattcggtg cagagagaat tttcatcgag aaacaatctg gcgcaacgat
      181 tactcatcga cctgttttcc aagaagcatt agattacgtc cgagatcaag atatttttat
      241 tgtggaagct attgatcgac ttggaagaaa ttatgacgag atcatcgctt cagtcaatta
      301 cttgaagaag aaaaatgtga agctgatcat tactagttta ccgatcatgg ctgaagcaat
      361 tggcaatcct ctcctggata aattcattaa ggatctgatc atccaaattt tggccatgat
      421 cgctgaacaa gaacggactg aatcgaaacg tcgccaggca caaggaatta agatcgcaaa
      481 agcaaacggc gtctacaaag ggcggcccaa attatacagt gcagacgcaa aagatcccca
      541 acgtcgcctt gtttacaaaa gcatcgttga agatcttaaa aatggtgttg cgattgctaa
      601 gattgctaaa gactacaatg ttacgagaca aaccgtgtat agaataaaaa aagacagcat
      661 ggtcaatgac aaatagttga tcatgctgct tttattatct tctcatcttt aatttcctac
      721 atataattac tatctataaa attaagtctt actcagaaca gataaagcat aaaattccaa
      781 caaaccattt tttacttaac taaataaaga gtctgttaat ataatcttca cttatttacg
      841 ttaatcccct actgtatttg ttggttcctt atctatattt tctacaaaat ccttattatc
      901 ttgtgattta gcttcgtata attctttgta tgtcgacaca ttggttatac taattcctat
      961 agctagttct tgagactcta cttttctctc tagctccttt gacaaagtga tttgagattt
     1021 atttggttcc gttggagtca attctataga ttcttcataa aaatctctat acataaagct
     1081 atcattaatt ttataaaaaa aattaaatgt ttttagcact gccatataac attcaaaaca
     1141 ttcttctata gttggtttac ttccttgagg agaatgaacc aatttattac gtttttctat
     1201 gaaattttta atttcctttc ttatttcttt cggaaactca atattaaagt accctttaaa
     1261 catgctccct aataatttgt ctacaggggg agattgtaaa ttctcaaata ttagagttaa
     1321 atcaggtttc tcttgttgat aaaattcctt tacccctatt tcacaggctg taattatcga
     1381 aataaaccta tctctagtat ttgatatatt tttacttctt ttaaggagta taagagaaaa
     1441 ggggatactt tcgtgtccat gcatgtaatt aactaattgt tgagcttcag aagttatatc
     1501 tctaattata tagttaacgg aattaggaag tgtctttaac tctaatgaaa tttctatcca
     1561 attactatct tctaatctgt atatgctcga agtataattt gattcgtcag caagcatcgc
     1621 tacatcccaa acactgatca taaaatcttt taaacgttca atcatttcag caatattgct
     1681 aaaatcagct aatttttctt tagaataaaa aaaacattta tatcagaatc aataaattct
     1741 atttttttta ttactatatc gtcatcatta tcttgaccat ttatgtttat tgttccctga
     1801 gtggggagta cttctttcaa attcaagtta aatttatatt ccattacttc tcctccaata
     1861 tataaaaatt acttaactgt atccaaattt aatatgtaaa taaatcatta atttttagct
     1921 aaattctatg agaagtttat ttttgttgtt tttgctaatt aactaaaact agtacaaaaa
     1981 atttaaaata gattattaaa cctattgctt attctattca gtcatatata taagcgtcat
     2041 cctttagtaa ctaacatccc acactacaat agtattataa catatctatt caacttcaaa
     2101 ttgttgaaat ttaagccatt tatatttact tttcctcaac tttagaaatg attttactaa
     2161 ccttatatcc gtaatacgcg taacaaacac attcattaat aacaatcaac actaagctat
     2221 attagctttt taaatgacta gggtaaataa aataaggatc aaaaactttt aagtttctga
     2281 ccctttcctt acttccgatt ggttaatagt gatcttttgc agaataaata aatatcgttt
     2341 cattttcaac tctatatatc agtctatgtt catctgtaat tcttctggac cattttccag
     2401 ataaatcatg ctttaatggc tcaggttttc ctaatccagc aaagggggaa cgatcgatat
     2461 cttttattaa cttgttaatc ttttttatat tgcttttgtt tccttgctca tgccaataaa
     2521 gataatcatc ccaagcatca tcagaccaag ccttaatcat cagattcaac ctcgattaag
     2581 tcatgtgttt taaatgcacc tttggagaat tgttcatctc ctcgacgaat tttttccatg
     2641 acgtaattat tagaaagtgt tctcaacgtt tcttgcatag aatcataatc tctttttgat
     2701 aatacaacaa ctgtatcttc tacatcttta cttgttacaa taagtgtttc agcatcctca
     2761 ttaacttgtt tcatataact acgtaaattt tggcggaaat ttgaataagc tactgcttcc
     2821 attcctttca ccccttattt atttctataa aacaattgta cattatattg tacaattaag
     2881 caaatgatta atattctaac ttcttata
//
LOCUS       DAJSAW010000145         2908 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJSAW010000145 DAJSAW010000000
VERSION     DAJSAW010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.151"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            511..1746
                     /locus_tag="ONT64_002863"
     CDS             511..1746
                     /locus_tag="ONT64_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304218.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQQRISE"
     gene            1887..>2908
                     /locus_tag="ONT64_002864"
     CDS             1887..>2908
                     /locus_tag="ONT64_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304219.1"
                     /translation="MKNTIFSDEYVEIIISDHSKNTKEIAKKIEEKKVFDGCHYLESK
                     SFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFDEFICFSMGEVWLEGIYACL
                     YRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLKKRTPLYNKSYNFYCFFPNI
                     YTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKYIFFTSVYDFEGGEPVGEYE
                     LVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSSIPWEAIQLSGDFNDKIFMT
                     INSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAYDIERLLKQKTMKNILKNVK
                     IAESLD"
BASE COUNT         1047 a          298 c          512 g         1051 t
ORIGIN      
        1 cagtatataa aatgagcgta aaaacggcta tttttgcctt aacaacgatt tttttgtata
       61 caaatacgat atgctcatta attattggaa tttgtagtgc tgtttaaatt aatgaaggtg
      121 taattatatg agtgatcatt ttgaaaaagt ttgttgagaa gatgtgcttc tagtcatgtg
      181 atgggagaaa ataatttaac tgttagagtg aatagttaaa ttattctggt tatatgaaaa
      241 aagtagtttc taaaataatc ttgcagggat taacttaaag aaattgagag aatagtttct
      301 ttgaaatttc tatatacaat gagaagctga aatttacttt tatttgatga ctggaaatag
      361 aaattaaata tattgtattg ttacattaaa gaaaacttgt atggaaaaat cagtacaata
      421 ccattattta atgttcttca cttaaagcgc aaaaaatatc agtttattaa actcgtatat
      481 agaacaaata tatgtagacg gggcgaaacg atgagtgtaa aaaaaatgct atttatggta
      541 agtacaataa ttgtattaac atcccgattt tggggaatta atactagttt atctatgcga
      601 tattttatta tggcaatatg ggatttatat tttatgttaa gctatttttc ttatcgtgag
      661 aaaacatata aaaaatacgt tatcaagaat aacttttggt tgactataat gccgtttgtg
      721 atattttcta tctatactct aataatatgg ggaattggtt caaatgcaga atttgaaaat
      781 tttataaagt tatgttccac gttgctttat ttaattcttg catatggtta cgcatgtaca
      841 gcattttact tatttaaggc agaaggaatt gattttattt tttggtcagg tgttatttca
      901 tatatgagtg gttcagtttt ttatttaatt gtatcttatg gtttaaatgg tttaatttct
      961 tatataaaaa gtcttataac aggtgaaaca aatactgcaa attttgctat ggaggtacat
     1021 gatttaactt ttgcgatggg ggtttttttc ctttattacg tattttttga aaataaaaat
     1081 acaaaaaaac ataagataaa aattatcgta tctttaattt taattttcat gggattgaag
     1141 agaattgaac tgttagcttt aagtggtgca attctagtgt attatgtttt attaaagtgg
     1201 ggaaaaaaga ttcaaatccg tgcattagtg tgtactattt gtgcaactgt tatttcgtta
     1261 gtgtttattt acattataga tacaggaatt ttggcattgc ttatgtcaaa tttgggcatt
     1321 acagatgacg gtgcgaggct ttcattttat ctatattcat ctaaatttta tgaacttggt
     1381 ttaggatatg taggaacagg atggacatgg ttttcaaaat acttttttaa tctttattta
     1441 tcgcacttta ggttaaatgg tggaagaatt gcagcaagcc tacacagtga tatattaact
     1501 atttatatag aagttggatg tatagttttt attatatgga tgttttggtt attttttgtg
     1561 aaaccgataa gactaactaa aaaatttgga ttgaaaacag gggagtgtgt attattatta
     1621 actatatata tgtttatatt atatttgacg gataatacgc tcacttatcc agacactcaa
     1681 atgatttttc tcttagtacc tatgatagtt tcgtgggttg ataatcaaca acgaatatcg
     1741 gaatagatag ctaaagtata ttttaattat gaatttataa ttagttgtaa aagttaatat
     1801 ttaacggaca ggaagtaggg tgaaaaaatt tgaaaaaaat tttagtgtta gcaaatacat
     1861 attatcaaat aatagtagca atacaaatga aaaatacaat tttttccgat gagtatgttg
     1921 aaattattat ttctgatcat tctaaaaaca ctaaagaaat tgcgaaaaaa atagaagaaa
     1981 aaaaagtgtt tgatggctgt cattatttag aaagcaaatc ttttgatgat aatattgggt
     2041 attttcaaaa gattaaggac tgtcttcaga tttcttgtgg taaatcaaat cgatatgcct
     2101 attatttgaa aaactgtaaa agtgaatatt ttgatgaatt tatttgtttt tcaatgggag
     2161 aggtttggtt ggaaggaata tatgcttgct tatatcgaaa taataaaaat gtacaggtat
     2221 caattttaga agaaggtatt ttaagctatg gagttgacat tattccaaca gcaagaacaa
     2281 aagctatcgc agtactcaat tacttaaaaa aaaggactcc actatataac aaaagctata
     2341 atttttattg tttttttcct aatatatata caggaagaga gaatacaata caaattccat
     2401 tagttacagc tgggtcaaga accgcattgc aactttcgga tatttttaac cctatgatag
     2461 atatttataa agaaaaaaaa tatatatttt ttacgtctgt ttatgatttt gagggcggag
     2521 aacctgtcgg agaatatgaa ttagtatgca aaatagcaga cttagtagga aaagataatc
     2581 taattgttaa gacgcatcct agagatgtaa gaacaattta taaagataat ggatttatcg
     2641 aggataaaaa ttcatcaata ccttgggagg cgattcaatt gtcaggcgat tttaacgata
     2701 aaatatttat gacaattaat tcaggaagtg ttatttcagg tagtacaatg tctgcaaaac
     2761 ctgtacgcac gtattatatg tataaattgt gtaacataga aggaaatccg tcttgcaaaa
     2821 agaatgcgta tgatattgag cgattgctta agcaaaaaac gatgaaaaac attttaaaga
     2881 atgtgaaaat agcagaaagc ttagatga
//
LOCUS       DAJSAW010000146         2894 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJSAW010000146 DAJSAW010000000
VERSION     DAJSAW010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.152"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(260..544)
                     /locus_tag="ONT64_002865"
     CDS             complement(260..544)
                     /locus_tag="ONT64_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304220.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
     gene            complement(565..1935)
                     /locus_tag="ONT64_002866"
     CDS             complement(565..1935)
                     /locus_tag="ONT64_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HCR2304221.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            2154..>2894
                     /locus_tag="ONT64_002867"
     CDS             2154..>2894
                     /locus_tag="ONT64_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCR2304222.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSQLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKG
                     ITISVPPRKNMDKSEKLDHSLLGKQRKTV"
BASE COUNT          912 a          590 c          439 g          953 t
ORIGIN      
        1 atgtgaaaat cgaatcttga gatagctatt ccccttgaga tattcactga tacttctact
       61 caaatcattt aaatttttat aattatttgt acggaacgta tatatatcaa catctattta
      121 gacgttgtcg gtgatacaat gtcatttttc ccgtgtacta tgtcatttat ctctatactt
      181 tgtcattttt ctttttgggc aatcaaaaaa ggcaacaaac caattatgat ttgctacctt
      241 ctccgtttaa aaacttatct tagccccaaa atggaatcca atttttaact tcttttgcca
      301 atgcatacgc cttagcaaga cctgagtttt caactaaata tttttcccct tccagagaaa
      361 cactccccca gatatgaaca gtgttatccg cccaaaccat ggattctagt aagccattgc
      421 gcgaaagaaa tttagtttgt tctaaaatat cttcttctgg aacttgccct ttaagctcaa
      481 taaaaggatt ctttttttca tcacgcaatt taagaattgc atatcttagt ttgttcttat
      541 ccaacctaac accacctcta tgctctattt cttatttctt agcgaatcat taatacttat
      601 tccgttatca tatttccagt agtcccaacc attccggcta ccccctaata gcagagctgc
      661 tgcattcgaa acagatgagg cagtgtaatc ttgaacaaat tgtagtcccc caattgtttc
      721 ttcaatgatt ccattatcca acagctcttg gcgagtttca tctagcgaat cagatgcaat
      781 atctttaatc ttagaataaa tttctgaacc tgaatgtata atcacggtct cgtcttcgtt
      841 caaataccca gttgctttta ttccttgcgc ctctaagaaa atttccctat tcccatcgtg
      901 ttcatagtcg cccgacgatc tcaaataggg atacatagta atcagttcat tgattagagc
      961 ttcacttctg gcagaaatat ccgatgatgt ccactcagtc aattcgtaca aatctttatt
     1021 catacgaata tgaagggtat cttcaaagat tttctttttg gtctcaaagt ctttatttcc
     1081 cgcggccgaa ttatcaggat ttgtaacgag tgttaaatta ccaatggtat ttactaatcc
     1141 tgtgtattct tcccccgatg taccagcctt ctcttcccaa tatgaagtac tagtctgtgg
     1201 catgatgtgt tcaatggaca agttactcat atcaagtgtt gctgaatttt ctcgattctc
     1261 tattttttca agtaaccaac gggccaatct catcgcataa gcattggcat tcaatagatt
     1321 agatttcaat gccttgtcag atggaagtgc catattattt gattgatttc tagtaacaag
     1381 aacataaatt acaatatcaa cgatattttc atatccatta gctttagcgt acttcctaac
     1441 acttttcaaa tatgaaggaa atgcttttga cactctgctt gtatcatccc cattaaaata
     1501 tctcctaagc tgataatcat tcactacctt aatcgcatca atatattggt cttcagtgat
     1561 aaattggtca tgatagtacc attcagataa tcccaaaaca aaaggtgcag gcatcatact
     1621 ttccatactt tggaaatcgc tcaatacttc tgagtatttt ccttcaggtt tgttatagta
     1681 aagtctcgca aaataaccag catatcgcac taatttttgg agcaattcgg catcacttga
     1741 tacatcgcgc tctttatgcc aatagtcttt gaatgcttga taaagaatat gctttgctga
     1801 atactcccca gttattgcag caaggtagta tctgaaaaag tcttccattc ctttagaatt
     1861 atcaaagact tcttccaacc tacgccaata tttagtataa agcgtagtct gctcttcatt
     1921 agatttatcc atcatgatga agtttcgaat caaatccgag gcagtcaatc tttcaccggt
     1981 agaattgata ctttcaaaaa tttgctgtgc attatcacgg tcatctaatt taatataaac
     2041 aatacgcgtg gtgctagtta attttttcct agaggaaaat gtctaaacat gttggtaaat
     2101 caacaaaaaa cgcatatagt agtctcaaaa ctcccaagaa atgaggacta catatgcgtt
     2161 catcaaaata tactgaacat tatacagata cttttataac attcaaggaa attatgagaa
     2221 ctgtttctaa catataccac aattgtgtac cagataagat taaaaaccga agaaatactg
     2281 atcaactgaa gcaacgcgat acagtgatta ttgcttgtgt tatatggggc ataattaatg
     2341 gctatactag ccaaagagcc acgtatagag cggtttgttc cgtcttattt cttaatggtg
     2401 actttcctag taggagtcga ttcactcgct taagttcaaa cttagcttac actatcaaga
     2461 ttattcgata ctttttcatc aagaagctca ccaaaggtga actagtcgga attatagata
     2521 gttttcctag tcaattatgt aaaccagtta gaaatagaca agcaaaacta ttgaatcaaa
     2581 tagccaaagt tggctataac tcaacaaaaa aatcttattt ttatggtctt aaaattcata
     2641 tgatcgttac taagactggc tttccgatta cttactcaat cacgaatcca ggtgtccatg
     2701 acgtgaaagt gttagagact ttatcggaag aagcgaatct tcccaatatc ctaggggaca
     2761 aaggatatat tagccataaa atccatgaaa agcttgccct taagggcatt actatatccg
     2821 ttccgccacg taaaaacatg gataaatcag aaaaactaga ccacagctta cttggaaaac
     2881 aaagaaaaac agtt
//
LOCUS       DAJSAW010000147         2820 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJSAW010000147 DAJSAW010000000
VERSION     DAJSAW010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.153"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(61..936)
                     /locus_tag="ONT64_002868"
     CDS             complement(61..936)
                     /locus_tag="ONT64_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2304223.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(1191..1275)
                     /locus_tag="ONT64_002869"
     tRNA            complement(1191..1275)
                     /locus_tag="ONT64_002869"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1239..1241),aa:Leu,seq:caa)
     gene            complement(1293..1364)
                     /locus_tag="ONT64_002870"
     tRNA            complement(1293..1364)
                     /locus_tag="ONT64_002870"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1330..1332),aa:Gln,seq:ttg)
     gene            complement(1374..1446)
                     /locus_tag="ONT64_002871"
     tRNA            complement(1374..1446)
                     /locus_tag="ONT64_002871"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1411..1413),aa:His,seq:gtg)
     gene            complement(1508..1592)
                     /locus_tag="ONT64_002872"
     tRNA            complement(1508..1592)
                     /locus_tag="ONT64_002872"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1556..1558),aa:Leu,seq:caa)
     gene            complement(1613..1683)
                     /locus_tag="ONT64_002873"
     tRNA            complement(1613..1683)
                     /locus_tag="ONT64_002873"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1649..1651),aa:Cys,seq:gca)
     gene            complement(1729..1800)
                     /locus_tag="ONT64_002874"
     tRNA            complement(1729..1800)
                     /locus_tag="ONT64_002874"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1766..1768),aa:Gln,seq:ttg)
     gene            complement(1812..1884)
                     /locus_tag="ONT64_002875"
     tRNA            complement(1812..1884)
                     /locus_tag="ONT64_002875"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1849..1851),aa:His,seq:gtg)
     gene            complement(1887..1960)
                     /locus_tag="ONT64_002876"
     tRNA            complement(1887..1960)
                     /locus_tag="ONT64_002876"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1926..1928),aa:Trp,seq:cca)
     gene            complement(1967..2047)
                     /locus_tag="ONT64_002877"
     tRNA            complement(1967..2047)
                     /locus_tag="ONT64_002877"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2011..2013),aa:Tyr,seq:gta)
     gene            complement(2049..2124)
                     /locus_tag="ONT64_002878"
     tRNA            complement(2049..2124)
                     /locus_tag="ONT64_002878"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2089..2091),aa:Phe,seq:gaa)
     gene            complement(2160..2232)
                     /locus_tag="ONT64_002879"
     tRNA            complement(2160..2232)
                     /locus_tag="ONT64_002879"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2197..2199),aa:Asp,seq:gtc)
     gene            complement(2242..2314)
                     /locus_tag="ONT64_002880"
     tRNA            complement(2242..2314)
                     /locus_tag="ONT64_002880"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2279..2281),aa:Val,seq:tac)
     gene            complement(2330..2401)
                     /locus_tag="ONT64_002881"
     tRNA            complement(2330..2401)
                     /locus_tag="ONT64_002881"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2366..2368),aa:Glu,seq:ttc)
     gene            complement(2416..2505)
                     /locus_tag="ONT64_002882"
     tRNA            complement(2416..2505)
                     /locus_tag="ONT64_002882"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2469..2471),aa:Ser,seq:gga)
     gene            complement(2545..2618)
                     /locus_tag="ONT64_002883"
     tRNA            complement(2545..2618)
                     /locus_tag="ONT64_002883"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2582..2584),aa:Asn,seq:gtt)
     gene            complement(2628..2743)
                     /gene="rrf"
                     /locus_tag="ONT64_002884"
     rRNA            complement(2628..2743)
                     /gene="rrf"
                     /locus_tag="ONT64_002884"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          829 a          652 c          536 g          803 t
ORIGIN      
        1 aatcatagtc catttactat gtgtcatttg gttgcgccta tagatagtta cgcccttatt
       61 ttaatagatt cttgctagat gaattgccaa atagatgatt tcattttctt gaatggcaag
      121 ttgaaattgc tgttcgatga tatctttaat ctttagtgct gtacaatacg ctttgctatg
      181 tttaaatgtt tcagtaaaaa aaccaatatt ttctactggc gtttcttcct gataaatcaa
      241 gcgtttaacc aggaatttga tatgaaccat taagcgatga taagtcagtg attctttatc
      301 ataaacgttt gaataggatt tttctagatg ggacaaaatc gtggtcgtca tttctactat
      361 ttttaagctc tttaaactat taggctctcc tagctcagca ttaatgatat gcatagtgat
      421 gaatcctact tcatttgacc ctaactcggt ttgatatcga tgattgattc gctcgaccac
      481 ttctactgct atatcatatt catttggata aaggaatctc atttctggca acataattgc
      541 tgtgacatct ctatcttctc tttttcgttg aatagcaaaa taaatatgat ctgtcaatgt
      601 aatatagata tttgaatcta atggagagga gatcctagct gttatcagtt caatcacttc
      661 ttccgcaaaa tccataaatt cgattggaat ctgcgagact atctcactga atcgtttgat
      721 cttttcttta gaatctaata caaataattt gtcagcatcc tcaacatcaa atggcttact
      781 cactttagca gaaaagccaa tccctttccc catcgcaatt ttttctattc catcgtcatc
      841 gatcaataca gcattatgat tcagcttttt catcacatac accgttaatt cacctcctct
      901 atatgataaa acaaaacaac ttgcagactc cttcatacat atttttgtct ccaaacaatt
      961 tttaaagaca aaaaacgacc taagaaagaa ccctttctta ggtcgtgcct aattgaatag
     1021 tagcacccta cataatttac aaaaatagtt taacacataa taaaagcgtt atcaatatct
     1081 tttttactcg ttttatgata atacttgtat cgaattgatt tggaggtaat tacataaaaa
     1141 caaaaaaaat atagcaactc tctataagaa agttgctata ttgataatta taccggcggc
     1201 cggggtcgaa ccggcacgtc ctcaacggac actggatttt gagtccagcg cgtctgccaa
     1261 ttccgccacg ccggcaaatt taaaaataat aactggggta gctggattcg aaccaacgca
     1321 tgagggagtc aaagtccctt gccttaccgc ttggctatac cccaagaata aagggcgaat
     1381 gatgggaatc gaacccacga atgccggagc cacaatccgg tgcgttaacc acttcgccac
     1441 attcgccatc ataaaagatg ttatttaatt tctcaataaa aaactaagat gaagacatct
     1501 tagtcaatac cggcggccgg ggtcgaaccg gcacgccctc aacgggcact ggattttgag
     1561 tccagcgcgt ctgccaattc cgccacgccg gcaagttgat ttattagtac taaggcggta
     1621 accggatttg aaccggtgat gaaggttttg cagacctctg ccttaccact tggctatacc
     1681 gccattatac tattatcaat ctataaaata aagaataaat tatagaaact ggggtagctg
     1741 gattcgaacc aacgcatgag ggagtcaaag tcccttgcct taccgcttgg ctatacccca
     1801 agaataaaaa gggcgaatga tgggaatcga acccacgaat gccggagcca caatccggtg
     1861 cgttaaccac ttcgccacat tcgccatggc aggggcagca ggaattgaac ccacactaac
     1921 ggttttggag accgctgttc tacctttaaa ctatgcccct ataaaatgga ggagagtgga
     1981 ttcgaaccac tgaaccctaa ggaacggatt tacagtccgt cgcgtttagc cacttcgcta
     2041 ctcctccatg gtggcgcggg acagaatcga actgccgaca cacggagctt caatccgttg
     2101 ctctaccaac tgagctaccg cgccatattt taaaattatt aattgaataa caagaacgac
     2161 ggttccgacg ggaatcgaac ccgcgatctc ctgcgtgaca ggcaggcatg ttaaccccta
     2221 caccacggaa ccagttcatt atggaggtta acgggatcga accgctgacc ctctgcttgt
     2281 aaggcagatg ctctcccagc tgagctaaac ctccaacatt caattaaaat gacccgtacg
     2341 ggattcgaac ccgtgttacc gccgtgaaag ggcggtgtct taaccgcttg accaacgggc
     2401 caggtaaatt tgctacggag agtaagggat tcgaaccctt gagacagttt ggcaccatct
     2461 acatgatttc caatcatgct ccttcggcct ctcggacaac tctccagagt gaaagaagct
     2521 caatactctt tctcaaaatg agaactccgg cagtaggact cgaacctacg acatcatgat
     2581 taacagtcat gcgctactac caactgagct atgccggaat aacatcagcg tggcgacgtc
     2641 ctactctcac aaggggcaac ccctcactac aatcggcgct aagaagctta acttctgtgt
     2701 tcggcatggt tacaggtgta tccttctcgc tatcgccacc acactgtggt gttatctttt
     2761 attgagtaaa ttttgttcac tcaaaactgg atttgaagtc acataagttt ttttccgagt
//
LOCUS       DAJSAW010000148         2672 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJSAW010000148 DAJSAW010000000
VERSION     DAJSAW010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.154"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..322
                     /locus_tag="ONT64_002885"
     CDS             <1..322
                     /locus_tag="ONT64_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2304224.1"
                     /translation="YKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEELVMLFLDPDFI
                     GKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLDGFGKPYPITH
                     LVKK"
     gene            736..1554
                     /locus_tag="ONT64_002886"
     CDS             736..1554
                     /locus_tag="ONT64_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR2304225.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1627..1986)
                     /locus_tag="ONT64_002887"
     CDS             complement(1627..1986)
                     /locus_tag="ONT64_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR2304226.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          925 a          372 c          471 g          904 t
ORIGIN      
        1 ttataaagca atcattcctg agaacttaga tgaggttaaa ctttatttat gggaagataa
       61 cggagaaatt gtcggtttta gtggtgtaag tgcagaagaa ttagttatgc tatttcttga
      121 tccagatttt attggtaagc gatatggtag tcaaatatta attgagctga ttgaaactga
      181 agatataaaa agaattgatg ttaatagtca aaataaatat gcaaaacatt tttatttaag
      241 ccatggattt gaaattgaat ctgaagaaga cttagatggt tttggtaaac cataccctat
      301 aacgcattta gtaaaaaaat aattcatgaa tggtttatta cctgattgaa catgaataaa
      361 agtaatattt ttattttgat ggcctatttc taggttatga attatccaga tcaaatttgt
      421 gtattacatt cagtaataag gtgcttatct taggatcaac ttatgatact agctgaccaa
      481 ggatgtacca gttatagaaa atcagttaaa gtaaaaatga taaaaatctt atgtcaacac
      541 tgatttaggc tgacctgtgg gttaggagaa agacaggtcg gtagctttta atacaaaaaa
      601 tggcttgtat gtatctagat agttaactga aattagaaat gttcttattt ttaacggagt
      661 aagaaaaata ggctgcttta ggtctgaata ctttttcaat ataatgaaga tatcaaaggc
      721 gacaggagtg tttttatgaa taagaatcct cataatctaa agttggtagc agtagatatg
      781 gatggaacat ttgctcgtac tgattacaca tacgatatcc caaggttcaa ggctgtttta
      841 tcgcgaatga aagctgttga ttgtcaattt gttgttgcaa gtggaaatca atactaccaa
      901 ttgagatcgt tatttccaaa ttattatgat gaattaagtt ttgttgctga aaatggtgct
      961 tttgttaaag ataaaacaga ggtactattt tcagccgata tgcctagaaa agcgatcgag
     1021 gtggtccttg atttatgtaa agactatcct gaagtaaaaa atgtgatgtg tggtctgaat
     1081 agtgcctact gtgaaagagg aaaggtgagt caagcatttt ttgatctaac caatatctat
     1141 taccatagat tagaatgggt agctgatttt gcagaagttc aagataaaat tttaaaattt
     1201 gctccgacta tgcctgttga aaaaaccgaa ttttacgttg aattgttccg tgaaaaatta
     1261 gccgggctat tagtacctac cagtagtggt catggttctg tagacttgat tctttctgag
     1321 tgccataaaa ctttcggctt gaaaatgtta gttgagcgct tggggattaa tcctgaccag
     1381 tgcgttgctt ttggagatgg gggcaatgac attgagatgt tggagtattg tggtctgagt
     1441 tatgaaatgg ataatgcaac ggaagctgtt aagcaagttg ctaagcatca atgtccttca
     1501 aatgacgaag acggtgtttt agtgacgctc gatcgattgt tccctaacac gtaaacacta
     1561 ggtaattcat caaaaaacct cgatataaga aatattcgag gttttaaaac tatatttaga
     1621 acagatttac gaagcgtctg atgagtcaaa atagacttgg acattgctat ctaaaatacc
     1681 actttcattc tttaaacgaa ttgtttgatt tgatggagtt acctctaata ctaaatgccc
     1741 attaataaag gtagtatcgt attcagatgt tttaactgtt gcttcttcgc aaggtattgt
     1801 taaatcatat gctggaatat aatgttgtgt ggaaactaat aaaaaatgat caccaatcat
     1861 acagcatccc tctgctgaag aaaattgatt agagccatca tttaaagtta acaagaatcc
     1921 atcctttaaa cgaggtactt gcttaaatac ttctggttgt acttctaaaa ataaattatc
     1981 gctcattttc acgaccacca tcgacttcca cagttgaaat tgacatttta tttaacacac
     2041 ctattccccc ttaactataa ctcaattcta tataaaaaat attctattct caaataattg
     2101 aacttttcaa atcgccaatt tacttcggct ttatcagagc tcattcatat caagagcgat
     2161 taacgctgta aaaattaaac cgaagatctg ataaattttc tctattttct acaatattta
     2221 aaacgcttcc ttagtctatt atatatctat ttctcttagg agtatgttta gatgtttgaa
     2281 taatagttgc ccaactttaa aggttattct tataatattt tacttcattt tccaatagaa
     2341 aattcaaagg attactggtt tttctaaaat agtttagttt aaatacataa gtatcctccg
     2401 acactgtatt agttcataat catctaaata caaaattttc aaataaaaat atttcaagct
     2461 ctatcgttga cgaatgtaaa cgatacttat aaagtaatag aaattgatta catttgtaaa
     2521 cttaaaatgg ttaatttatc tcacataaaa ctaagaataa aataatgatt aaaagtggtt
     2581 agtttttata cgatttagcc tatcggtaaa aatgaattag agagtgtaaa atattttgtg
     2641 taaatgaaaa aatccataca aaaaaggaag tc
//
LOCUS       DAJSAW010000149         2527 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJSAW010000149 DAJSAW010000000
VERSION     DAJSAW010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.156"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1211)
                     /locus_tag="ONT64_002888"
     CDS             complement(<1..1211)
                     /locus_tag="ONT64_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR2304227.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKM"
     gene            complement(1201..>2527)
                     /locus_tag="ONT64_002889"
     CDS             complement(1201..>2527)
                     /locus_tag="ONT64_002889"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCR2304228.1"
                     /translation="SISPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFD
                     DVDLQSKKLGTDEQQRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLISQFASEAGKK
                     AGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHG
                     QELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANW
                     SADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEG
                     VIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKN
                     QNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDM
                     VHVGNDIKKIRQEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT          703 a          531 c          424 g          869 t
ORIGIN      
        1 aacatctttt gcaagaagcc ttttttcgtt tccttcagca aatctaactt acgttgatga
       61 agagcgatcg tatcatctag ctgcttgaag aaggcaccaa ttttttgttg ttcatttata
      121 tccggtggaa actgaaatgt cctttgttct ataccacctt tagtcacatg taccattgtc
      181 gttccgttag ttgattcttt aattttctct ttatcagtca atagccaagt atatagatac
      241 tgtttgtcca attccttaga taaaaattta agtttccaaa tatgataatg gtagattaca
      301 cgttcttcat tccacatttc aggaccgaag cttgttgccc ataaatataa gagatctcct
      361 ttattagcat acttgtcttc ctctaattct aagtttgaat aataccatct atcattagta
      421 ttaaaattac ctactcttaa aaccctatat tttccagaat caagtaattc ttgttgttta
      481 aaagctctac cattcaaaaa aactaccact tcccccaact tccgctcttc ccaatctccc
      541 gtaaatcctg gaaaacgaac ttctggcact tttgctccgt ttttagggaa cattttttgt
      601 aagaatcctt ttttcgtttc cttcagcaaa tctaattcgt gctggtgaag catgatggtg
      661 tgatcaagtt gtttgaaaaa tgaaccgatc ttgatctgtt cgtcataagt tggaagtgag
      721 ataggtgtct ttttaccact atcaattgta taggttccga cggttccaat gcctgcaacg
      781 tcacggatat acttctgaat tttgttattt atgaacgagt agtaaaagaa gtttccgtca
      841 attttatgct cattcttgaa ccaaatgatg tttccatcct taaagtaaat tgggttatca
      901 tcagtgatta ataaggggac acctattgat ccgacaccag ttacaagcaa gtctccttga
      961 gaaactttac ctgaattttt cgaatattca ttatattttt cttctgaaat gtaaagataa
     1021 tcggatggtt cctcattttt tgcagctgag acaatatcac gagcacgtaa aaatctaata
     1081 cctgagttag tccaatctga ttgatgaatt cgcttcacag aagtaacatc catcatatct
     1141 cctaacttac gctcttccca atcttccgta aaaccaggaa accggatttc tggtcgtgta
     1201 tcattgctca tgtttgaaca cctccagcgc accttgtaac cacgcttcat tttcaggtgt
     1261 tgtttgcagg gaagagatag cttctaaaag ttctttttct agcacttgct gttcttgtcg
     1321 tatcttcttg atgtcatttc cgacatgaac catatcaatt ggttcttctt cttcgaaagt
     1381 atcgacatag cgtgggatat ttaagttata gtcattttct ttgatttcat caaaggttgc
     1441 caaatgagcg tatttctcga catcttttct ttcagcgtag gtttcgagga ttttttgaat
     1501 gttttcttcc gataatttat tctgattttt ccctttaaca aattcacgac tagcatcaat
     1561 gaataataca tctcgagttt gacgattctt cttcaataca atcaccgtcg ttggaatcga
     1621 tgtaccaaag aacaagttcg caggcatacc gatcacagca taaatactgc cgtcttctaa
     1681 taatttttga cgaatgaccc cttctgcagc accacggaat aaaaccccgt gtggcaagac
     1741 gatagccata gtccctgttt ccttcaaatg atagaaacca tgaagaagaa acgcaaaatc
     1801 tgctttggat tttggcgcta atttcccgta gcgattaaac cgagaatcat ctaagaaggt
     1861 cgtatctgct gaccaattag ccgaataagg tggattcatc actactgcat caaatgtgta
     1921 aggttcatct gttggccagt ccttattcaa cgtatcccca ttgcggagat tcatttcttc
     1981 ggcatctacc ccatgtaaaa tcaagttcat ttttgctaga ttgtatgtcg ttgtatttag
     2041 ttcttgccca tgatatttaa cgttatctgg atgagttaag tagttacgta catttaacat
     2101 caaggaacct gaacccatgg ttgggtcaaa gacactaaag aatcgtcgtt ctttttgatc
     2161 caacgtcacg atttgtgcca tcatatcaga aaccatatga ggcgtgtaaa attctccggc
     2221 tttcttccct gcttctgaag caaattgact gatcagaaat tcatacgcat ccccgatcac
     2281 atccccatcg tgacctaaaa cttccacgtc attcaatttc ttgataactt cggtaatcgt
     2341 cacatttcgt tgttgctcat ctgttcctag tttttttgat tggagatcca catcatcaaa
     2401 taacccatta aattgattgt aggtagatgc caattgcaca aaggctttgt ttagatcgtt
     2461 caattggaac gttgcttgtt ttgcttgatc cgctaagacg ttgaaaagat actctggtga
     2521 gatcgaa
//
LOCUS       DAJSAW010000150         2522 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJSAW010000150 DAJSAW010000000
VERSION     DAJSAW010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.157"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            5..2377
                     /locus_tag="ONT64_002890"
     CDS             5..2377
                     /locus_tag="ONT64_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304229.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         1010 a          398 c          555 g          559 t
ORIGIN      
        1 ggacatggca gaagaaaaag aaccactaga caatagtcgc ttgggaaaat ccaaaagaaa
       61 attagtacga ttgcaaaatg aattaaacga acaaattgag aagatgtttg aacaccagag
      121 aaagaccaac ggtcagccaa tgaacgataa gagaaatggg cattcttggt ttaggcaaca
      181 agaacgttta gaaaacaaag tgcatagtct gagagaagag atcaagcaac aagaaaaaca
      241 ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 cctggatatg acaatcgaaa atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg actttttcag ccgctacgat ccgaaaatac caacggaaat tggaaacatt
      421 agaacagctg aaagaaagat cagaaaaagg aaaagaaaat cttcttcctg aagtacaagc
      481 aattattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 agactatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctgc
      661 gccaactgat tctttgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tagaattgat
      781 cgatcaactc aaaacgttgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaagaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccca tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 aaaggaacaa aaacaaacgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaagcaaga
     1081 agtcaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtgaaa gaacttttga attttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ctgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actacacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgggg ggcatatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cttcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctgtagaaac ggcagaacaa aagaatattg ttcctgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccacagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 ttcattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 ag
//
LOCUS       DAJSAW010000151         2454 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJSAW010000151 DAJSAW010000000
VERSION     DAJSAW010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.158"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            132..344
                     /locus_tag="ONT64_002891"
     CDS             132..344
                     /locus_tag="ONT64_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304230.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="ONT64_002892"
     CDS             506..1024
                     /locus_tag="ONT64_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR2304231.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="ONT64_002893"
     CDS             1031..1543
                     /locus_tag="ONT64_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304232.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1814..2437
                     /locus_tag="ONT64_002894"
     CDS             1814..2437
                     /locus_tag="ONT64_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2304233.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          944 a          310 c          408 g          792 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt agttttaaaa
     1561 taaactaatg tccttatttt tgtgttcaca tttctgttac ctaagaaata cttaaaggag
     1621 gtgatgtagc cagcaacgac tacaacttga acccgctatc tacagtttat ttgttgtttc
     1681 ttttgggtat actctaatgg tacaaatcca tatttgctta aagcgttgat ttttcagcgg
     1741 tttttcttta ttgcaaagtg taccctaaga cctgtatact aatagtaaat agattggata
     1801 ggaatgatgg caaatgtatt tagcttattt aagagtttct tccgacgatc aaagtttagc
     1861 tcggcaacat aagctaattg aaaattggtc agtcaaaaat aatattgctg aagacgattt
     1921 aaaaattttc gaggaaaaag tctctggaaa aaatattgaa aacagaccag agctgcaaga
     1981 gcttatttct ttcatacgtg aacgagatac gattattatt cctagtcttg atcgattagc
     2041 aagaaatagt aaagatacaa aagacttact tcaattgttt cgtagtaaag gtgcttctat
     2101 tgaaatatta gacctaccgt cgtttaatgg ggtaactgac cctgcattaa gagacttgct
     2161 aacaaattta gtcatagaag tgtttagcta tgttgctgaa aacgaacgga aaaaaatcaa
     2221 agagcgtcaa aaacaaggca ttgaaattgc aaagaaaaat ggtcgttata ctggtaaaaa
     2281 gattcaatac catgccaatg caacaggaaa aaacaaattg atttacgatg aaattgttga
     2341 tggtctgaag aaaaaagaaa ctatttctaa aatagcaaag aacgcaggag ttacaagaaa
     2401 aaccgtttat cgagtaaaaa gagatttagg aatgtaatat gttccagtta gaaa
//
LOCUS       DAJSAW010000152         2446 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJSAW010000152 DAJSAW010000000
VERSION     DAJSAW010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.159"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(976..1266)
                     /locus_tag="ONT64_002895"
     CDS             complement(976..1266)
                     /locus_tag="ONT64_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304234.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1474..2166)
                     /locus_tag="ONT64_002896"
     CDS             complement(1474..2166)
                     /locus_tag="ONT64_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR2304235.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          717 a          450 c          348 g          931 t
ORIGIN      
        1 tagaatttaa taatttccat agcataaagt gtttagaaaa gaacattaag aatagttcta
       61 taataattct tgaactccat gtcttataat gttctatcga agcacccaaa tagccaagtt
      121 catctaatct acttgcataa actacgtcat ccgctctcat agtcggcata tacatattaa
      181 agagtaaaaa gataccccaa atccctatta gtaacaagat cagttctttg ttattcgtta
      241 tttttctatt atgtttgttc atttttatac tccttcttat atttcctaat agagtataac
      301 atagctttaa gcattactta aagataactt aaaagttatc ttttaaatac ttaattgtta
      361 aataccttat ttgatgcaaa agaaatctac gatagtcaac aaaaaaattt tcattatatc
      421 cagaaaaatt tttcttagtt aaggacctat ggattgcagt aatgcagaat tcgtcgaata
      481 aactgccaaa gacatacaat catattgctt ttaaaattga cgaacaagat atagattcgt
      541 ttgtctcaaa aatccaaatg ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag
      601 gcgaggcttc ttcaatttac ttttacgact atgataatca cctcttcgag ttacataccg
      661 gtactctcca ggaacgttta aaatcctaca attccactac ctgagactag taatgactag
      721 tctcttttta tggcctctga tccccccatt gagcgaatac taatagggct tcgaattgat
      781 ctgttcaatc tttaagcaat atccctcaaa tgagtttgga aagtttagtg attcatcaca
      841 aatacttaaa tacatgtagt tatatataga ttgaggttct tgtgagcgta tgctcgtgtg
      901 ggttcaaatc ccatcgtctg catccatctt tccaaaacaa gcggctagga catttttgtc
      961 tcagccgctt gttttctagt ttattctttc atcttgtcgg accttttttc ttgggtatag
     1021 acttttctca tattctacca caattttggg tttccctaac acatgatagg atctcacaac
     1081 aaattgcttt ctattattat aataacctgt aacgactaac ttcatccctt cgtctacttc
     1141 agctaggaag ttcaaactat gggaagcaat caaacaattg accccattta aactgaaacg
     1201 gatcaacggt ctggcactaa cttgcaaagt acggatctta tctactgttc catatttttt
     1261 ctccataacc ttcacctcaa taccattata cgaacataca ttccctttgt aaagaagaaa
     1321 ctctgtttta tctttttgac tttttgtcta ttacctttct taaaatcata ttggcacttc
     1381 ctgcgaagat gaaggacatg tactaaaaaa aagaggcagc agtatagatt actgttgctt
     1441 ctttactaat ttaggtttac ttgttaagaa agattattta cgcaaatgtt cttcaacaat
     1501 ttgctgagat ttatcagctg tatcttccac ttcatctttt gctttttcta acttttgaat
     1561 atattgtttc aagcttgagt tttcagcagt tgcttcgtct aatttctttt gtgtatctgt
     1621 taactgagtt tctacatttg ttttttcttg tgtcagtgtt gcgatttgtt gattcaaacc
     1681 atttacagta ttttgtaatt ctgaaagttt tgcttgtgct tcttgtagtc ttgctgtcca
     1741 atctgcattc agatgattga tttcatcatt tttttggttg atctcatcta gtttgacatt
     1801 gatttcattt tgtttgcttt gtagttgtgc atccttgctg actaattgac tttctaagtt
     1861 ttgtttttct gctgccgatt gatttttttg gttttctaat tcatttactt ttgtttggta
     1921 ttcttggaca tctgcctttg aatcttgcaa tgtctgttct ttttgtgcta gtcgagcggt
     1981 aagatctgta agcgcttgct ctttatctgt caatgtatga tcaagctgtg tcagattctc
     2041 attgattgta tcaatatctg tctctccacc ccacaaaata atttcatttg cgtaccgcgt
     2101 gcctgtgtag ccaattagac atagaaatgc aacgacaatc ccaccagtaa taaatttctt
     2161 tttcatttgc atagctcctt aatccaatac ttttacatgc taaaagtaag tgatctcaaa
     2221 cacaatgtca agcgttttta aattacatgt gtaattttaa tataaatact atcaaatact
     2281 catttaatcc tcctttttta tctatttaaa aaatcttttt tatagagaaa aatgcaggtt
     2341 aatactttct taaataaata tttatggtga acaaaaacaa aaaagcaagt tttattaaac
     2401 gtaacccttt tcatttcata gattactgta tttattacaa aaaacg
//
LOCUS       DAJSAW010000153         2417 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJSAW010000153 DAJSAW010000000
VERSION     DAJSAW010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.160"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            113..2266
                     /locus_tag="ONT64_002897"
     CDS             113..2266
                     /locus_tag="ONT64_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCR2304236.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          824 a          425 c          535 g          633 t
ORIGIN      
        1 attttatctt gaaattaggt aaagaatttc ttgctccgcc cattttttct tttttattaa
       61 gaagtttttg ggtacaatga agcatgaatg taaataggcg gaaggatagg aaatgatgaa
      121 agattttatg aaaaaaatat taaatacttc ttggttagaa aaaataagaa aaggtgcaca
      181 agaacctaag aacaaaacgt ttcgccggtc ccatgttcct tttcgcttaa attttctttt
      241 tttcattata tttacgttat ttgttgcgtt gattgctaga ttgggttatt tgcagatcgt
      301 gaacggcgaa gagatggagg cgcgagtgaa gtctacttct acgattacgg tccaagaaag
      361 tactcctcgc ggaatgatct acgactccac aggaaaagct ttagttacga ataaagcgaa
      421 tcaggccatc acatatacga gaggaacaca agttacggct aaagatttgc ttgaaatcgc
      481 aacgaagctg aactcgctga tcgatgttcc ggtagatgaa aatctgacag atagagataa
      541 aaaagatttt tggctggctg atcccaaaca tttgaaagaa gccacaaaaa gactttcggc
      601 aaaagaaaag caattaggga acagcgaaca atatagtgcg atggtcaata aagtcacagc
      661 tgatgagatt aatttaaacg aggagcaatt aaaaattgct accatcttta aacggatgaa
      721 tagtgcatat gaattaaaca ctgtctatat caaaaactct ggtgttactg atcaagagtt
      781 agctgttgtg gcagaacgcg cttctgaact tccaggagtt tcgactggca cagactggac
      841 acgagaatat aatgcagcaa gttcactgaa aagtattctt ggcagtgtga cgactgaaaa
      901 gcaaggactt ccagcagatg aagcagagaa atacctagca aaaggttatt ctcggaatga
      961 ccgtgtagga caaagttatt tggaaaaaca atatgaagat gtcctgcaag gaaccaaaac
     1021 ccaatatgaa gtttcccttg ataatgaagg aaatgtttcg aatcaaaaag aaatcttctc
     1081 tggggaaaaa ggttctaatt taatgttatc tatgaatgca gaattccaat caaaagtaga
     1141 agaaattttg aaaaggaatt atcaaacatt gatcaacaac ggcaaagcac agtattcgcc
     1201 tggtgcttat gcagtggcta tgaatcctca gacaggagaa gtgctagcaa tgactggttt
     1261 cagtcatgaa caaggttcca aagaaatcac cgagaatgct ttaggcacga ttactagcgc
     1321 ctttgatcca gggtctgtag tcaaggctgg tactttgaca gcaggctggg caagtaacgc
     1381 gatcagcgga aatcaagttt tgattgatga gccgattcgt ctgcaaggtg cttcagaaaa
     1441 aagctctgtc tttaatcgaa gcggacaagt tgctcttgat gcagtcaaag catcggaatt
     1501 gtcttctaat acgtatatga tcaaagtggc gttaaagatg ttaggactag actatacacc
     1561 tggaatggga ctacctagtt tagatgaaga agctaaagct tatcagcaat tgagagactc
     1621 tttcaaagag tttggtcttg gtacaacgac tgggatcgat ttgccgaatg aatctcctgg
     1681 gatctcacgt tctgttgatt atatgaagaa gttcaatgcg gataatggca aagaatggta
     1741 tgctccaggt aactttaccg atttggcatt tggacagttt gacacatata caccaatcca
     1801 attagctcaa tatgcttcaa cagtcgctaa tggcgggaaa cgagtacagc cgcacctagt
     1861 caaagcgatc tatggcaatg acgaaaatgg aaacttgggt gaagtgaaga aagaaattgg
     1921 cacaacagta gaaaatacgg tcaatatttc tgcagaaaat atggcgatcc tccgtgaagg
     1981 attccaccaa gttgtccatg gtaccgatcc ttacacaaca gccaaaccat tagccagtgc
     2041 gaaaatggac ctttccgcaa aaacagggac tgcagaaacg gttgcggaag gtcatccaga
     2101 tattgcgaca gtcaatagta atatcgtggc atacggacca acagacaatc cggaaattgc
     2161 catcagtgtt gtcttgccga acttattaga cgaacaagac cacataaact tgacgattgc
     2221 taaagaaata atggacgcgt attatgatat gttcatggca aaataaaaaa gaggtcgtga
     2281 aaaagccgct tagtttcgag tatgaagaaa aattttgtga aaatagcttc tcacattttt
     2341 gcttaaattt gacttcatac cgagaaactg gcttttgaac accgtttact cggcaaaaaa
     2401 ggttgtgaca aaaattg
//
LOCUS       DAJSAW010000154         2407 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJSAW010000154 DAJSAW010000000
VERSION     DAJSAW010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.161"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            5..1150
                     /locus_tag="ONT64_002898"
     CDS             5..1150
                     /locus_tag="ONT64_002898"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR2304237.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="ONT64_002899"
     CDS             1321..1779
                     /locus_tag="ONT64_002899"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR2304238.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="ONT64_002900"
     CDS             1938..>2407
                     /locus_tag="ONT64_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCR2304239.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DAJSAW010000155         2400 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJSAW010000155 DAJSAW010000000
VERSION     DAJSAW010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.162"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            207..2069
                     /locus_tag="ONT64_002901"
     CDS             207..2069
                     /locus_tag="ONT64_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2304240.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="ONT64_002902"
     CDS             2069..>2400
                     /locus_tag="ONT64_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2304241.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAJSAW010000156         2233 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJSAW010000156 DAJSAW010000000
VERSION     DAJSAW010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.167"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            221..838
                     /locus_tag="ONT64_002903"
     CDS             221..838
                     /locus_tag="ONT64_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2304242.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            879..1178
                     /locus_tag="ONT64_002904"
     CDS             879..1178
                     /locus_tag="ONT64_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HCR2304243.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1212..1379
                     /locus_tag="ONT64_002905"
     CDS             1212..1379
                     /locus_tag="ONT64_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR2304244.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1424..2029)
                     /locus_tag="ONT64_002906"
     CDS             complement(1424..2029)
                     /locus_tag="ONT64_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2304245.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          738 a          362 c          418 g          715 t
ORIGIN      
        1 atggcagacc gttaaggcct gccattttta tgtattttag cggaataaat ataatcatgc
       61 gggagtgtaa acttactgtt tcaaaaagta tactttttga catgccttga ttttctttgt
      121 ttatgtatgg cataataacc acataataca atttgaaacg tttctgattt aatctgattg
      181 ttttgaaaca attttagtga aaaaagggta gataaaaacg atgagaaaat ttttttatat
      241 ccgggtcagt agttatgatc aatcgatcga acgacaactg atttccgcga aggaattagg
      301 aatccctgaa gaatatatat ttgtagaaaa agccagcggc aaagatttta aacgccctga
      361 atatcaatta atgaaacgga tgtttcgaaa aggagattta ctctatctac aatcattaga
      421 tagactaggc agaaataaac aaatgatttt agaagaatgg cgagaattaa ctaaggaaaa
      481 gcagattgat atttctgtgt tagacatgcc tctgttagat actgctagtt acaagcaact
      541 agatgggtta gagacgctta tctctgactt aattctgcaa ttactcagct atatggccga
      601 agacgagcgg aaacgaatta atagccggca aagagaaggc gtagaagctg cgaaacaaaa
      661 gggtgtaaaa ttcggcagaa agaaaattgc tttacctcct gaatttcctg cgatctattc
      721 tgactggaaa gcaggaaaga taactgcagt ctatgctatg gatcaattgg gcatgaaacg
      781 aaataccttt tatcgacggg tgaaagaata tgaacatacc ttgcaccact tttcttgata
      841 gatataacag ctattagacg ggaagggaga gtttgaaaat ggacttaacg aatcaaaggg
      901 tcgtttttac tggaacgctg cagcaaatga cacgaaccca agccatcggt ttggttcact
      961 ctttaaacgg ccattgtcaa tcaagtgtta cttcaaaaac aaatttcctg gtttgtggtc
     1021 agtattctaa gtcgttgttt gatgattctc tctatacaaa aaaggaacaa accgcgcggg
     1081 acttgattgc gttaggtcat gagattacca ttttatcaga agtcgaattt tatgcgttaa
     1141 ttagtcaaca gttgaaagaa tggcaacggt atttataatc gttagtaaaa tttacaagga
     1201 ggaaaatttt tatgaagcga aaatcttatg atcgttgggg cattgaaata actagtttta
     1261 atcgaaatat tgcttttcac gtggagtgtg actgtgggga attagcaaaa agtgtttatc
     1321 aaaaaaatta cttttactgt cctaaatgcc atcgccgata cggggtacat ggaacataga
     1381 aacgaaatag tgcggtagaa aagcgaatga atgtagtata tttctacaaa tcattcgctt
     1441 tctttatttt ataaatagtt ggtcgagaga tcccgttttc ttttgcaatc tctgcaactg
     1501 gaatgccttg ctttaacatg tccaccacgg ttttaaaaac taagcgtttt tgcggatctg
     1561 ttgagttaat agaatactcg attggtcgtc ccttatatcg gccggctttt tgagccaata
     1621 atactccttg ccgctgacgt tcacgaattt tctcacgctc gttttgtgca atataactta
     1681 gcagtgataa aaacatatcg aacatagcgg tatccaggag ttcatttcca gtattaaaat
     1741 ctaaaaactg tgcatctaat atttttaacg ctactttttt ctgtttcata aaagcaactg
     1801 tattttttat atcttcataa tctcttccta aacgatctag tgatgtaacg atgacgcaat
     1861 caccttcacg gatataattt agtaattttt gaaactcagg acgctcagta tctttaccac
     1921 tggctttttc ttggaataat ttatcaatgt taagtccttc catcattgcc aattgtcgat
     1981 ccaaattttg atcttgtgaa gatactcgaa tatagccaat ttttcccata aaaaactcct
     2041 ttttaaaagt gatataattt acatgtaaaa tttacttgta gttttactgc tatcatagca
     2101 tttttaaaaa taaattttat gtagacttaa aatttacttc atggagggtt taaatggatg
     2161 aaatcaaaag taacaaaaaa gcatttggca tttatttagg agaacctagt acagaacaat
     2221 taaaccaatt ttt
//
LOCUS       DAJSAW010000157         2232 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJSAW010000157 DAJSAW010000000
VERSION     DAJSAW010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.168"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..544
                     /locus_tag="ONT64_002907"
     CDS             <1..544
                     /locus_tag="ONT64_002907"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2304246.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="ONT64_002908"
     CDS             complement(900..1472)
                     /locus_tag="ONT64_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2304247.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /locus_tag="ONT64_002909"
     CDS             complement(1488..2093)
                     /locus_tag="ONT64_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HCR2304248.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          700 a          454 c          338 g          740 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tttgcacagt ct
//
LOCUS       DAJSAW010000158         2143 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJSAW010000158 DAJSAW010000000
VERSION     DAJSAW010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.169"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..351
                     /locus_tag="ONT64_002910"
     CDS             <1..351
                     /locus_tag="ONT64_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR2304249.1"
                     /translation="SVKKQFGKYSNLEINSNDLATYILRYDDKIIEIHLDYFGRVPTR
                     KFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWND
                     LYHANEVLKIAEEK"
     gene            352..1104
                     /locus_tag="ONT64_002911"
     CDS             352..1104
                     /locus_tag="ONT64_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCR2304250.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            1109..2056
                     /locus_tag="ONT64_002912"
     CDS             1109..2056
                     /locus_tag="ONT64_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR2304251.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          761 a          279 c          416 g          687 t
ORIGIN      
        1 tcagtaaaaa aacagtttgg taaatattcg aatcttgaaa ttaacagtaa tgatttagca
       61 acatatatat tgagatacga tgataaaatt atagaaattc atttagatta ttttggaaga
      121 gttcctacaa gaaaatttca attgtttact ttagatgaat gtatcaatgt tgacttatta
      181 aataataaaa taagttttga agaaaacatt gttaaagaaa tgcctgaatc tgctaatgat
      241 atgtatgaga aggagttgaa atatttcttt gaaaacgtag taacaggcaa agaaaactgg
      301 aatgatttat atcatgcaaa cgaagtattg aaaatagctg aggagaaata aatggaaatt
      361 ttatttacta tttgtggtag agctgggtct aaggggttta aaaataaaaa tttgaaatat
      421 ttggcaggaa agcccctagt ccatttcgct ttagcaatga tagatatcta tcaacaaaaa
      481 catcctgaac ataattgcac gacttgtttg aatacggaca gtaatgagtt agtagaattg
      541 acgactaagt taaatccgtc tgtgaaaatt gtagaacgta aacctgaatt agctacagat
      601 actgtaggga aaattgaagt gatacgtgat tcattcttac aactagaaag agaagataat
      661 atatttgatt taattataga tttagatttg acttcacctt tacgtgaagt ttctaatctt
      721 gaagaattaa tagatgaaca tctaaaaaat ttagataaag acgtctactt ttcagttgtt
      781 tctgcaagac gtaatcccta ttttaatatg gtaaaaagaa ttgaaaatga agttactata
      841 gttaataaat caagatacac atctaggcaa cagacacctg aagtttttga aatgaatgcc
      901 tcaatgtatt cttttaaacc tgaatttcta aaaaaaagta attatatttt tgatgggcaa
      961 tgtgggataa taaaaatgag agattatcta attttagata ttgatagtga agaagatttt
     1021 aaatggttag aatttttgtt tcctaaattt ttagaagatt cttcggagct tcaagatatc
     1081 tataattatt tatatcggag gtaaaaggat gagctgttat cttatcaccg gtggggctgg
     1141 cttcattggt tctaccttag ccaatcattt aggagaagaa catgtggtaa tcgtcgtcga
     1201 tgacttatca atgggaaaag tagaaaatct tgatatgacg aaaaatatca cttttattga
     1261 gggagatgtc gctgagaaat ccttgatgga agagatcatg aaagctaata aatttgatta
     1321 tatctttcat ttagcagcag ttgctagtgt ggcagattca gttgcccgac cagtagaaac
     1381 acatcgtgtg aattttgaaa gtgtattaat gattttagaa ttgatccgca agtatcaacc
     1441 tgatttaaaa cggatcgtgt ttagttcttc agcggctgtt tatggggatg aaccgacttt
     1501 accaaaaaaa gaagagtccg tgattcgccc attgacgcct tatgcggttg ataaattcgc
     1561 tgcagaacaa tatgtgctag attattgtca tttatatgat gtacccggta gcgcagttcg
     1621 ttttttcaat gtctatggtc caaatcaaaa tccgaattct ccatacagtg gtgtgatttc
     1681 tattctcgta gatcgctata agaaacaaat cgtaggagaa gcaacttcat ttacattatt
     1741 tggtgatgga agccagtcta gagactttgt ttatatagat gatgtaatcc aagcattact
     1801 tttagttgcg aatgaggaag ctgcactggg gaaacaattc aatgttggaa cgggtaaagc
     1861 aaccacactt ttggaattga ttcatgcaat caatgaaatt ctcggtgtag aattgacact
     1921 tgaatacaaa gaagagcgtt cgggtgatat ccatgactct ctagcagata ttactaagat
     1981 ccagtcaatt ggctatcaac cgaaatatgg tattttaagt ggaatgacta attatttgaa
     2041 aactgaaatc aattaaaaca tactaagata agattagagg gtaaattcag gctctttgtc
     2101 aataaggact gatgagtttt gtaaaatcaa atctgggata gaa
//
LOCUS       DAJSAW010000159         2065 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJSAW010000159 DAJSAW010000000
VERSION     DAJSAW010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.170"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1008
                     /locus_tag="ONT64_002913"
     CDS             <1..1008
                     /locus_tag="ONT64_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304252.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="ONT64_002914"
     CDS             1025..1174
                     /locus_tag="ONT64_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304253.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="ONT64_002915"
     CDS             1171..1617
                     /locus_tag="ONT64_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304254.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="ONT64_002916"
     CDS             1619..1756
                     /locus_tag="ONT64_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCR2304255.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2065
                     /locus_tag="ONT64_002917"
     CDS             1794..>2065
                     /locus_tag="ONT64_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304256.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          753 a          358 c          425 g          529 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa acttt
//
LOCUS       DAJSAW010000160         2002 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJSAW010000160 DAJSAW010000000
VERSION     DAJSAW010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.171"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..378
                     /locus_tag="ONT64_002918"
     CDS             <1..378
                     /locus_tag="ONT64_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCR2304257.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="ONT64_002919"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="ONT64_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR2304258.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2002
                     /locus_tag="ONT64_002920"
     CDS             1859..>2002
                     /locus_tag="ONT64_002920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304259.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          554 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tt
//
LOCUS       DAJSAW010000161         1911 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJSAW010000161 DAJSAW010000000
VERSION     DAJSAW010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.172"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..969
                     /locus_tag="ONT64_002921"
     CDS             <1..969
                     /locus_tag="ONT64_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR2304260.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="ONT64_002922"
     tRNA            1159..1232
                     /locus_tag="ONT64_002922"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          639 a          327 c          362 g          583 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
     1261 ttagaatttt tttgaagagt attatacaag ctaaagcttt tcttcattgc cactcaaatg
     1321 agtggctttt ttatgtatcc ttttatggat taatgaaagg atgtttcaca tagttatact
     1381 tctgtatatt tgaaaagttt tactttgatt tttaaataga aagacatttg ggttaaattg
     1441 tgagataata ataaagaaga gtttaaagcg caccccaaac cacttcccca taagtgtgtt
     1501 acgctttaaa ctcttttata tttgaagcca ttaaaaagca taccatattt ttgaaaaaaa
     1561 gtgagaaaaa aggcttacaa ttggagtggt agttaattag tgacttattt ttgattttat
     1621 agcactgata ctataaaata tagatatcat catattacac aatcttaata ctaacttaaa
     1681 aaatatctcc tttcacaagt atggtgataa aattcgttcc gggctacctt tttaggtagc
     1741 ctactttaat ctttatacct ttctggatca acgaaagtat actttatata gtcataacgc
     1801 cgatgatcgc tacgtgcgtc cggcacgtca gtcatgaatc ggctacacta gactagacag
     1861 aaaaaataag gtgtgtagaa taataaaaaa cacactggag gatttttcat g
//
LOCUS       DAJSAW010000162         1865 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJSAW010000162 DAJSAW010000000
VERSION     DAJSAW010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.173"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(243..464)
                     /locus_tag="ONT64_002923"
     CDS             complement(243..464)
                     /locus_tag="ONT64_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304261.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="ONT64_002924"
     CDS             complement(562..807)
                     /locus_tag="ONT64_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304262.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1047..1661
                     /locus_tag="ONT64_002925"
                     /pseudo
     CDS             1047..1661
                     /locus_tag="ONT64_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          626 a          324 c          275 g          640 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt
      901 ataaactatt taacttttag aattcaaaaa ccgtttgtct atttagagtt taccctaaat
      961 agacaaaaat ataacattga aaaatttaga gttctaacgt atgattaggg tgtattataa
     1021 acgttataga aaagtaggga ataaatttga aaattgctta tattagagtt tcatccatcg
     1081 atcaaaatga acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa aaaattttta
     1141 tcgaaaaaca atctggagcg acgattagtc atcggccgat tttccaggaa gctttagatt
     1201 ttgtcagaga gcaagatatt tttattgtag aagccattga tcgacttgga cgcaattatg
     1261 acgagattat cgattcagtg aattatctaa agaagaagaa tgtgcgactt attatcacca
     1321 gtttaccgat catggctgaa gcaattggaa atcctctcct agataaattc atcaaagact
     1381 taatcattta aatctcagcc atgattgctg aacaagaacg tactgaatcg aaacgacgcc
     1441 aggcacaagg aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt cctaagttat
     1501 acagtgcaga caccaaagat cctcaacgtc gccttgtcta taggagtatc gttcaggatc
     1561 ttgagaatgg ggttgctatc tcaaagattg caacagacta caatgtgact aggcaaacaa
     1621 tttaccgaat aaaaaaagaa atcgaccaac taattgtatg atttcttttt tgaatcacgt
     1681 aattattaat tagctttgtc atcaatacag acaatcattt tagattcatc tagctaagtt
     1741 gattacattt atgttaaaaa gaatttagtt aacataaata taattgatgc ataaatcgtc
     1801 cttatttaaa ggcatttaac attgacaact tagtataatg tatcttatgt aaactaataa
     1861 gattt
//
LOCUS       DAJSAW010000163         1796 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJSAW010000163 DAJSAW010000000
VERSION     DAJSAW010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.174"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="ONT64_002926"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="ONT64_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCR2304263.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          503 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DAJSAW010000164         1743 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJSAW010000164 DAJSAW010000000
VERSION     DAJSAW010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.176"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..528
                     /locus_tag="ONT64_002927"
     CDS             <1..528
                     /locus_tag="ONT64_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304264.1"
                     /translation="ENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIF
                     AKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPIST
                     ISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLP
                     ENWAELRKLVEYGII"
     gene            515..>1743
                     /locus_tag="ONT64_002928"
     CDS             515..>1743
                     /locus_tag="ONT64_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCR2304265.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKK"
BASE COUNT          569 a          226 c          277 g          671 t
ORIGIN      
        1 gaaaacagta aattgatgaa aaacatacaa tcaatctttg ggatagaaga agtttttgtc
       61 ggagataaat gtatcttttt tgatcaacct ttattatctg atggttatgg catagatgaa
      121 acagatattt tcgctaaagt aaatagtata ttttctggaa aaaaagaaac tttaattaag
      181 cttcatccta gagcaaggac aaatcgttat ggtgaaaaaa acatattgaa ttccagttta
      241 ccgtttgaag tcttgctact tgaaaatgac ttctcttcaa cacttttgat tagtcctata
      301 tctacaattt ctttttcacc atctatgatg tttgaaaaga cgatcaactc tattatgttg
      361 gctaaattaa ttttaaatga atatacagca ttatctgaag ataaaaaaga aacattgaca
      421 aatattgttc ttttctgtga acattataac cgtctatcag aagaaaaaat tttactccca
      481 gaaaactggg cagaattaag aaagttggta gagtatggga ttatttaaca cactttccaa
      541 gtcaaaatta gttaagtcca gtttctggta tactataggg aattttttta ttaagggaat
      601 ttcattcata actattccta tttttgtaaa tattactact caagcagaat atggtttgat
      661 taataatttc acttcatttg tatcaatttt ttcgattctt gttgggctaa gcctaaattc
      721 ggcaattaat aatgcaagtt ttgactataa agatgacata aatgaattta tgtcatcggt
      781 actttctcta gctacacttt cgttaattgt gttcttatta atagggaaca tatatttttt
      841 gttttcaaat gctttttttg atatgaacca acttgtattt aatttgatgt tattacaaag
      901 ttttgctagt tttgtaatta gttatatcca gtcttatttt acgattaact ttaaccattt
      961 taaatatttg attttatctt ttatatctac ttttttgaat attgcaatct ctgtagcttt
     1021 gatgcagaca ttatattttg atgataaata tgttggcaga gtagtcggcg gaacactgtc
     1081 atttgtgttg ataggtttag taatttattt aagaataatg ataaagggtg ctcatttttt
     1141 tgataaaaaa cagtggagat acgctttaaa aatctctttg ccgctaatcc cacattcctt
     1201 agcaaatata gttttagcac aattcgatag aattatgatt aattcttatt taggtcctgc
     1261 tgcgggggga atatattcct acattagtaa cataggtatt attctaagcg taatatggat
     1321 gtctactaat aacgcctggg ttccatggtt ctatggtgag atgagtaaaa ataatgaaaa
     1381 aaaagtgaag aaagttagta actattattt agtagtcttt actttgatta cgatgattgt
     1441 aatggtagtt tcgattgatt tagcaaaatt tatggctccg aaagactatt attctgggtt
     1501 atctttagtg gttccgataa ccttaggcta tttctttcag tttttatata gtttaccagt
     1561 taatgctgaa ttttatgaga aaaaaacatc gtttattgcg attggtacag tagcaagtgc
     1621 tatagttaat atactattaa acatgatttt catacctagg ttaggaataa ttgctgctgg
     1681 atacacaacc gtatttacgt attttttact ttttattttt cattataata tcgctaaaaa
     1741 aat
//
LOCUS       DAJSAW010000165         1709 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJSAW010000165 DAJSAW010000000
VERSION     DAJSAW010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.177"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..156)
                     /locus_tag="ONT64_002929"
     CDS             complement(<1..156)
                     /locus_tag="ONT64_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2304266.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEI"
     gene            complement(153..806)
                     /locus_tag="ONT64_002930"
     CDS             complement(153..806)
                     /locus_tag="ONT64_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2304267.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1094..>1709
                     /locus_tag="ONT64_002931"
     CDS             1094..>1709
                     /locus_tag="ONT64_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2304268.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLR"
BASE COUNT          546 a          311 c          235 g          617 t
ORIGIN      
        1 aatttcattt gccatattat gaatcttttc ttttctcgtc tccaaatatg ttttaggaaa
       61 gataaaaaaa attaatgaat gaactaatat aattattatt ccaagaacag aaaatatttg
      121 tatgaacatt tttggaaata tctttaattt tttcattttc tctccaattt atatcctaca
      181 tttcttatag tagttataca gtctaattgt aatttttttc taagttcttt tatatataca
      241 tctattactc gatcataagg agtttcttcg ctatctttcc atacataatc aataatttgc
      301 attcttgtta acacttgtcc attattatcc aataaacatt ttaatacttc tagttctttt
      361 gcatttacat ctatttcttc accatttatt ttagctgtat agctattaaa attaaccgat
      421 agatccttat attcaaactt ctctaaatgt ccataattct tcttaattaa agaatctatt
      481 ctagctttta agaccggcaa tgaaaatggc ttttctacat atccgtctac taaattagta
      541 aatgcatcaa ttttatattc ttcatcacta aatgcagtca agattagaat tggtaaattg
      601 cttttctttc taatttcttt caacacttct aaaccattta taaaaggtat ctgaatgtct
      661 aagataacca aatttatatc gctattaaat tttgacaagg cttctcttcc atctttagct
      721 tgaataacag tatatccaaa ttctgaaaga tattcactta ttccctctct tatcatatta
      781 tcatcttcaa cagttaatat tttcatatat acctccactt aagcctatac actagatatt
      841 atacagcata ttatggctct ataatatcta gtgttggtga caccttctgt tgccaatact
      901 ttttcttttt tctaataaat gcaaacagac accttgaaat cctttagaat aaagggttct
      961 ataatatttt gtgttgttgg aaaatcgaag gcgattttct actaacactt tttcttcgtt
     1021 tcaactcaaa aagagactca cttttggtaa aatttagtcg accaaaacat caccctacca
     1081 aaggagtcgc tctatggata atcatactag aaaattactc ggattaacag ataaacattt
     1141 attttttgat gaagaatggc tgattgaaaa agaatataaa ggcgttcaag cgcagtttat
     1201 taaagggaga ttggatgact caccttccca ttgtgaacac tgtggatcga ttcgtattat
     1261 ccgcaacggt tcctatacga cacgtactca gatattgaaa gtcaaagaaa agttgactat
     1321 tttagaatta aaacgtaccc gttttctatg ctatgattgt gggcagacct tttctgcgaa
     1381 aacggactta gttgatgaac atcatcaact aacaaaggaa ctgaagcagg cgattctcat
     1441 agaactgtac gaaaatcaat cacgtaaact aatcgctaag aagtacttcg tctcggatgg
     1501 gacggtgaca cggatcttgc gtgaagcaac gaagcattac cagccacgta tgaacttcct
     1561 tccgacggtg ttgtgtatgg atgagttcaa atcaatgaag tctgtttctg gttccatgag
     1621 ttttatttgt gttgatggga caacccatca gctatttacg atccttgaag atcgcagact
     1681 ttacaaacta actcaatact tcctgcgct
//
LOCUS       DAJSAW010000166         1602 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJSAW010000166 DAJSAW010000000
VERSION     DAJSAW010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.178"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..589
                     /locus_tag="ONT64_002932"
     CDS             <1..589
                     /locus_tag="ONT64_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR2304269.1"
                     /translation="VDIPKWVEEKKNKGIITRTNYSDLLRLSLLAKHGGLWLDATFYC
                     TKPIPDEYFDIDIWSIRRPDYAHASVASGYFAGYSLACNQESRWMFAIIRDFFLNYWK
                     NNDMMVDYLMIDYMIVLAQRINHEIAQTFDKIPSNNPLCDELIKNLNNIYDEKVWNLL
                     KKDTLLFKLTWKQSFKKQKDDKVTFYGMLVENKLL"
     gene            611..>1602
                     /locus_tag="ONT64_002933"
     CDS             611..>1602
                     /locus_tag="ONT64_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR2304270.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILL"
BASE COUNT          628 a          161 c          313 g          500 t
ORIGIN      
        1 tgttgatata ccaaaatggg tggaagaaaa gaaaaacaaa ggaatcatta cgagaacaaa
       61 ttattctgat ttattaagat tatctttgct agcaaaacat ggaggtttgt ggttagacgc
      121 cacattttat tgtacaaaac caataccaga tgagtatttt gatatcgata tttggtctat
      181 taggaggcct gattatgctc acgcatccgt agcatcagga tattttgcgg gttattcatt
      241 agcatgtaat caagagtcta gatggatgtt tgctataatt agagactttt ttttaaatta
      301 ttggaaaaat aatgatatga tggtggatta tttgatgata gattatatga ttgttttagc
      361 acagagaata aatcatgaaa tagcacagac atttgataaa atacctagta acaatccatt
      421 atgtgacgaa ttgattaaaa atttaaataa tatatatgat gaaaaagtat ggaacttatt
      481 aaagaaagat actttactgt ttaagttgac atggaaacaa tcttttaaaa agcaaaaaga
      541 tgataaagtt actttttatg ggatgttggt agaaaacaaa ttattataaa tattagaggt
      601 ataaaaagtt atgaaacaca aggtaagtgt gatagtacca atttacaatg ttgaaaaata
      661 tttaagaaaa tgtgtggata gtttattgag tcagacatta aaagagatag aaatcgtatt
      721 agttgatgat ggttcgccag atgcttcggg ggaaatagca gatgagtatc aaaaaaaata
      781 ttctaatgta aaaacaatac atagagaaaa tggggggtta ggaccggcaa gaaatacagg
      841 aattgagaat gcgacaggag aatatgtggc ttttcttgat tcagatgatt gggtagaaag
      901 tgatatgtat gaaaaattat acttagttgc gtccaaagaa aatgctgata tagtagttag
      961 tggacattgt gattttgcga atgacaaagc tatggtgaaa aagcaacatc ctttgagtgg
     1021 gaatgtatat gatacgccta atagtattaa aaatgtacga aaaaatttat ttggacatag
     1081 tccaaaagat caagaagtag aggccttccc gatgtcagta tgtatgtctt tgtataataa
     1141 aaatctgcta gataaatata gactaagatt tgaaaaaatt ttatctgaag atacaatttt
     1201 taacttgaat gcatatgatg tcgcaaataa aattgctttt acagaatata cagattattg
     1261 ttatagaaaa gaagaacaag agtctattac aaaaagttta tctccaaata agcagacgga
     1321 atttaaagaa tttttaataa ctctaaaagc tatagcatcg aaagaagatt tagaatgtca
     1381 aatcagagca aaacgtatgg caattgatta ttgtaggctt tatgtgggct tagttgatga
     1441 tgcaaatttg ggaattaaag agaaaagaaa gtatataaaa aaatttattg aagatgaggg
     1501 attaattcaa tgttggaagg gatatcctac aaatacgcta ccatttcaac aaaaattttt
     1561 tcataaaatg cttgaatgta agtgttatac aatattgttg tt
//
LOCUS       DAJSAW010000167         1577 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJSAW010000167 DAJSAW010000000
VERSION     DAJSAW010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.179"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            138..752
                     /locus_tag="ONT64_002934"
     CDS             138..752
                     /locus_tag="ONT64_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2304271.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(776..1126)
                     /locus_tag="ONT64_002935"
     CDS             complement(776..1126)
                     /locus_tag="ONT64_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR2304272.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1120..1383)
                     /locus_tag="ONT64_002936"
     CDS             complement(1120..1383)
                     /locus_tag="ONT64_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HCR2304273.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          520 a          286 c          245 g          526 t
ORIGIN      
        1 atactatttc ctccatcatt agtttttgtc tatataaggt ataccctaac tagacacgac
       61 cgcgacattg aaaaatctgg ttttctcgtg tatgattagg gtgtttttta aatagttgca
      121 gaaagtaggt aaaaattttg agagttgcat acatacgtgt ctcatccatc gatcaaaatg
      181 aacaacggca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc attgagaaac
      241 aatctggcgc aacgattact catcgacctg tttttcaaga agcactggat ttcgtccgag
      301 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattac gacgagatca
      361 tcgcttcagt caattacttg aagaagaaaa atgtgcagct gatcatcaca agtctaccga
      421 tcatggccga agcaattggc aatcctctcc tggataaatt cattaaagat ttgatcattc
      481 aaattttggc catgatcgct gaacaagaac gtaccgaatc taaacgacgt caggcacaag
      541 gcattaagat tgcaaaagcg aacggtgttt ataaaggacg tcctaagcta tacagtgcca
      601 atgcaaaaga tccccaacgt cgtcttgttt ataaaaacat cgttgaggat ctaaaaaaag
      661 gtgttgcgat cgctaagatt gcaaaagact acaatgttac gagacaaacc gtatatagaa
      721 taaaaaaaga cagcatggtc aatcacgaat agttgatcat gctgctgttt ctattttatt
      781 tgcttacttt ttgaaaaact gttctaactt tcatcaataa ctcctcaact aaactatcat
      841 gcgctttttc caaaaaaata cactctctgg cttcataatc aattgtcgtt agttgatcta
      901 ataatacttt tcctgttgtc ttcatttcat attctgagtt tatatttaca tatagcggat
      961 agtttctagt tgtactagaa attggagcaa ctgtaacaac gttactataa tccgcaataa
     1021 tatcatgact taaaacaata taaggtcttt tacctgtctg ttcgtgtcca gatcgaggtg
     1081 ctgtattcaa taatagaata tcgccttgtc taggttttct caccattttt cattacctac
     1141 tgcttcacct aaattttgaa tcgtcacatg atttctttca tcagtgtaat tttcaaataa
     1201 ctgttcaatt gttaattcat tttctatatt agccgcttcg ataattagcc gcttctgacc
     1261 gtttacttct tcttgtatta tcttcacttt gtcttctgta tgaattccaa gatattccat
     1321 aatatacttt ggcaatctaa ggccattact atttccccat ttttttatag gtaacatttc
     1381 catagtagac acctctttct gtatatacat agtataacat acataagaaa aaacgtatat
     1441 acttaattat ccttttctat taaattagat taacattcca tgaatccata aactatgtta
     1501 acatttatat gaaaatagtc gaaaggaaat cataatatgt ttaagaaaac aaaatcttta
     1561 gaatcaggta agaaaaa
//
LOCUS       DAJSAW010000168         1573 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJSAW010000168 DAJSAW010000000
VERSION     DAJSAW010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.180"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
BASE COUNT          594 a          179 c          320 g          480 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taa
//
LOCUS       DAJSAW010000169         1509 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJSAW010000169 DAJSAW010000000
VERSION     DAJSAW010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.181"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            101..868
                     /locus_tag="ONT64_002937"
     CDS             101..868
                     /locus_tag="ONT64_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR2304274.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="ONT64_002938"
     CDS             1060..1458
                     /locus_tag="ONT64_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR2304275.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          561 a          189 c          282 g          477 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatg
//
LOCUS       DAJSAW010000170         1479 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJSAW010000170 DAJSAW010000000
VERSION     DAJSAW010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.183"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            191..445
                     /locus_tag="ONT64_002939"
     CDS             191..445
                     /locus_tag="ONT64_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR2304276.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="ONT64_002940"
     CDS             446..763
                     /locus_tag="ONT64_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR2304277.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          527 a          217 c          283 g          452 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattaaccc
     1381 gtggtgctag ttaattttaa tatcctaaag aatatctcaa agttccttca aaacgtcgtt
     1441 gtgctcgact taatagaaca ctgtaagcca gtaatatgc
//
LOCUS       DAJSAW010000171         1468 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJSAW010000171 DAJSAW010000000
VERSION     DAJSAW010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.184"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            287..694
                     /locus_tag="ONT64_002941"
     CDS             287..694
                     /locus_tag="ONT64_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304278.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="ONT64_002942"
     CDS             708..1109
                     /locus_tag="ONT64_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304279.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..>1468
                     /locus_tag="ONT64_002943"
     CDS             1111..>1468
                     /locus_tag="ONT64_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304280.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKII"
BASE COUNT          562 a          178 c          258 g          470 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataa
//
LOCUS       DAJSAW010000172         1453 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJSAW010000172 DAJSAW010000000
VERSION     DAJSAW010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.185"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..625)
                     /locus_tag="ONT64_002944"
     CDS             complement(<1..625)
                     /locus_tag="ONT64_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HCR2304281.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="ONT64_002945"
     CDS             complement(784..1050)
                     /locus_tag="ONT64_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2304282.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="ONT64_002946"
     CDS             complement(1040..1396)
                     /locus_tag="ONT64_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR2304283.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          406 a          279 c          181 g          587 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg aat
//
LOCUS       DAJSAW010000173         1425 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJSAW010000173 DAJSAW010000000
VERSION     DAJSAW010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.186"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..285
                     /locus_tag="ONT64_002947"
     CDS             <1..285
                     /locus_tag="ONT64_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304284.1"
                     /translation="DRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFD
                     KVGWPSRPPQSRNWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            295..498
                     /locus_tag="ONT64_002948"
     CDS             295..498
                     /locus_tag="ONT64_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304285.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            709..1293
                     /locus_tag="ONT64_002949"
     CDS             709..1293
                     /locus_tag="ONT64_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2304286.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          501 a          205 c          270 g          449 t
ORIGIN      
        1 gataggaacg gaatagccat tggtaaccca actaacgact taactgttac tgaaaattat
       61 ttaatagttt ctccattact tgctcgagca gcttctaaaa cgatacgagt gacaggaacg
      121 atgatttttg ataaagttgg ttggccttct agacctcctc aatcacggaa ttggaaagaa
      181 aaaagaaatg gttattggta ctatggaaaa ttgtatttac aaagttataa atcagctgga
      241 aacaattgga ttgcgactta ttcaggaacc ttaactagaa agtagggatt ttttatgcca
      301 gaatttgtga acacaccaaa tttttatcaa gatagcgata ttctaagtaa aattaattta
      361 ccgaaagaag aatcagcttt acaagttcag cgaactttac aggagacaca aaaaattcaa
      421 aaatcaattt ctaatctaga agattctgaa gaaaaaagaa aaaaaagaga ccaaaaaaca
      481 tatcaaaaag aaaaataatg attttaaaaa aagagtgtct agtcgacatt ctttttttct
      541 aattgaaagt gttcataaaa tcacccattt ctggacactt cctttttttg ctttattttt
      601 caaaaaaaca attcatttca tgttcataaa cccataacaa tatctatgtt cagttaaact
      661 atatggataa agttatgtta caataaaatt aatgaaggag tgattttttt gaaaatagga
      721 tacgcccggg tatctactgg tttacaaaat ttggatttac aagaagacag cctaaataaa
      781 tttggttgtg aaaaaatttt tactgatcat atgagtgggg caaaaagtaa tcgaccagga
      841 ttagaaatgg ccattgattt tgttcgctct ggcgatacac ttgtcgtttg gcgcttagat
      901 cgtttaggta gaaatatgga agatttgatt tcaatcgtca atcgactgaa tgagcgtggt
      961 gtgagttttc atagtattca agaaaatatt acgatggaca aatcaagttc tacaggacag
     1021 ctcatgtttc atttgtttgc ggcatttgca gaatttgagc gtaatttgat cctggaacga
     1081 tccgcagcag gacgagcagc tgctcgtgca agaggacggt ttggtggtcg tcctgaaaaa
     1141 ttgtctgaac aagatttgga attactgaag actttagtgg atagcggtac acctattaag
     1201 actattgcgg aacggtggaa cgtctcaaga accaccattt atcgttattt ggcaaaaatg
     1261 gaggagaaaa atggaaagac aaattctcat tagtttttgt catttaatgc tgaaaatttt
     1321 tgccatttat ctagaaaagt gacaatagta tgagaatttt ttgtaagtgt ctactgataa
     1381 cttattctca aatgttgtaa ggattttatt catttgttaa tatta
//
LOCUS       DAJSAW010000174         1417 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJSAW010000174 DAJSAW010000000
VERSION     DAJSAW010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.187"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(150..1337)
                     /locus_tag="ONT64_002950"
     CDS             complement(150..1337)
                     /locus_tag="ONT64_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HCR2304287.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAJSAW010000175         1412 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJSAW010000175 DAJSAW010000000
VERSION     DAJSAW010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.188"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            99..587
                     /locus_tag="ONT64_002951"
     CDS             99..587
                     /locus_tag="ONT64_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR2304288.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
     gene            574..>1412
                     /locus_tag="ONT64_002952"
     CDS             574..>1412
                     /locus_tag="ONT64_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2304289.1"
                     /translation="MEKIKQTFPLINKYWNKKKIFIFFITFLLSSLFSLIFPYLFSNV
                     IDLYLENELSQNIVRIVTILIFQLFLVLIKEVYGYIKTIYAFEVDLSMKIDFLKVLQK
                     KNLFFSQTKHSGEIQFRMFSDISTIVNTIFIFSVELPVIGVLVLVAVFIISKVNLIFL
                     IFSLIMMGAMVLQYNLFQLPLKKTIENTKSVNQSITGELNAHFDQLEIISAYNFENIS
                     ILKFDSRFKELFNVFKKQNKLLTLGSCLAQVIFQLWIGGIFLIDVILLAKGTISITNF
                     VIV"
BASE COUNT          523 a          128 c          230 g          531 t
ORIGIN      
        1 tggctaataa aatagtattt cttatattgt atagtttttg tttttatgtg aaaacatgag
       61 caatagaatt gtactagtag gataaggatg gatggaaaat gaaaaaaact atttttgttt
      121 tagcgatttt tatatgtttt gggctacttt tggtaggcta cttgttgttt aattctaatg
      181 aaatgagaac tatttctacg aatgacttgt taaaaaaaag tgaattaaag gataaaaaaa
      241 aagaatctct tgcctttata atgtttactc aagaagagtg tgagtattgt atagcaataa
      301 atccaatcat agatagtgta aataaggaaa ctttgaataa gataaagaaa tatgatatat
      361 ctgtagattt tcaatctaaa caaaagtgta aaattaagtt tgttccgact attgttgcat
      421 atagtaagaa taaagaagtg gggagaattg acaatgaatt agataatttg tatcgaaaaa
      481 agcaagatgg ttcaattgta atagacaaga aaaaaacaaa gacattgctt atagattgga
      541 tgaaaaaaat ggataaacaa ggagaatatt tgtatggaga agattaaaca gacatttccg
      601 ttaattaata aatattggaa taagaaaaaa atttttatat tttttattac gtttctgctg
      661 tcatctttat tttctctaat tttcccgtat ctatttagta atgttattga tttatattta
      721 gagaatgagt tatcacagaa tattgtgcga atagtaacga ttcttatatt tcagttattt
      781 ttagtcttaa ttaaagaagt gtatggatat ataaaaacta tttatgcatt tgaagttgat
      841 ttatcaatga aaattgattt tcttaaggtt ttacaaaaaa aaaatctttt tttttcgcaa
      901 acgaagcatt caggagaaat tcaatttagg atgttttcgg atatttctac tatcgtaaat
      961 actatcttta ttttttctgt tgaattacct gtgataggcg ttttagttct tgtagcggta
     1021 tttattataa gtaaagtgaa tcttattttt ctgatttttt cactaataat gatgggagct
     1081 atggtgttac agtataattt atttcaatta ccgcttaaga aaacaatcga gaatacaaaa
     1141 agtgttaatc aaagtattac aggagaattg aatgcacatt ttgatcaatt agaaattata
     1201 agcgcatata attttgagaa tatctctatt ttaaaattcg attctagatt taaagaatta
     1261 tttaatgttt tcaaaaaaca aaataaatta ctaacactgg gaagctgttt ggcacaggtt
     1321 attttccaat tatggattgg aggaatattt cttattgatg ttattttatt agctaaagga
     1381 acaataagta ttactaattt tgtaatagta tt
//
LOCUS       DAJSAW010000176         1391 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJSAW010000176 DAJSAW010000000
VERSION     DAJSAW010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.189"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            49..510
                     /gene="mscL"
                     /locus_tag="ONT64_002953"
     CDS             49..510
                     /gene="mscL"
                     /locus_tag="ONT64_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCR2304290.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(570..1016)
                     /locus_tag="ONT64_002954"
     CDS             complement(570..1016)
                     /locus_tag="ONT64_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2304291.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(1109..1354)
                     /locus_tag="ONT64_002955"
     CDS             complement(1109..1354)
                     /locus_tag="ONT64_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCR2304292.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT          407 a          293 c          251 g          440 t
ORIGIN      
        1 tttatttttc gatatactaa agacaaataa aagaagggag ggtcattcat gatcaaagaa
       61 ttcaaagagt tcatcatgcg tggagatgtc ttggatcttg ctgtcggcgt tgttattggt
      121 agtgcgttta caagcatcgt aaaccaagtg gtgcaaggct tgatcacacc actaataggt
      181 tggtttgtcg caatgatcac aggtacttct gatttagaag gagcactttc aattcttgac
      241 tggtcgccga caaaaggcgt cacatttgcg tttggtaacg ttattagtgc tatcattaca
      301 tttctgatca ctggttttgt cttattctta gttgtcaaag cggcaaaccg tgcgaagaat
      361 ttacgctcca aagaagagca agtggaagtg gaagaaactc ctactgcaga agattatctt
      421 agtgacatac gcgatcttct tgccaaacaa gtggcagaag acaatacaca tccagtagag
      481 caaacaaatc tttccgatac ttctaaataa taaagaatct aaaaaaaaag aaagccgatt
      541 ttcagtgtcg gctttctttt tttagattct taacttgcta cttgttcctt tttcatgtcg
      601 atatgtggaa tatcatcttc caaatacaca tctgagattg ccttgaatcc aaacgactca
      661 taaaaagctt gtaggtattc ttgtgcgccg atcacgattg gtttttctgg aaaatttttc
      721 tggatcatgt taatcgtttc ttgaacaatc tttcttccta aaccatcttt acggttgtcc
      781 tgatgaacaa ggacacgtcc aaaatggata ctctccgctt cttcatatac tcgcgtgtag
      841 gcaaggatct tcccatcagt atcttgaaaa aacgtatgcc aagcttctcc atcaatgtca
      901 tcaacttctt gataaggaca ctgttgttcc acaacaaaaa ctgcgattcg ttctttgacg
      961 atcgcgtaaa actctgctgt tgacaattct tcaaatttct ttacctgata ttccactaag
     1021 caaacgcccc ctgctttttt attcttagca taccgattcg cacacaaaaa agcaaacgaa
     1081 ccttacgaat ctcaggctcg tttgcttttt aaactgcttc agttaaaaat tcgatctcaa
     1141 ctgtccgagt aagtacatct gaatagctgt aagatactcg ttcaaaagaa ttttcttcag
     1201 gatccaaatc aacaacgaat actgaaggat aggtttctgt taaaacgccg cggcgttctg
     1261 tttgacgctt tcttcctgtc tgagcaacca acatgattgg gctgccgata cgaccttcta
     1321 aatctttctt gattgaagct aaagttgttg gcatacagtt cacctctact atcaactata
     1381 ccacatattt t
//
LOCUS       DAJSAW010000177         1372 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJSAW010000177 DAJSAW010000000
VERSION     DAJSAW010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.191"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            122..>1372
                     /locus_tag="ONT64_002956"
     CDS             122..>1372
                     /locus_tag="ONT64_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR2304293.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          428 a          271 c          261 g          412 t
ORIGIN      
        1 gctctatact ttttgatgtt ggtgaacttc accaacatct tttttgtata aaaaaatata
       61 gaaaagacac cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa
      121 gatgtctcat actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt
      181 cgaggaaaat tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg
      241 aactcttctg tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat
      301 cattcgttgg gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac
      361 ttatctggag ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc
      421 ggatacctct gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat
      481 tgctcgctta aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac
      541 ttcttcggtc tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac
      601 tttgccaaag gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat
      661 gagttttatc atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg
      721 cttgccttat ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca
      781 attgattgtt attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa
      841 tgctcagttg atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa
      901 tcatcgagtg aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt
      961 agggaaaaaa ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta
     1021 tgaaaaacgg gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga
     1081 tcgactgtta gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct
     1141 ttatgcggta aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag
     1201 cggtctgcca gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat
     1261 ccaaaatgcg cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat
     1321 caaagtatta aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DAJSAW010000178         1314 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJSAW010000178 DAJSAW010000000
VERSION     DAJSAW010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.193"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..848
                     /locus_tag="ONT64_002957"
     CDS             <1..848
                     /locus_tag="ONT64_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR2304294.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          429 a          240 c          254 g          391 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttttta acggaaatct atcctatact aaat
//
LOCUS       DAJSAW010000179         1296 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJSAW010000179 DAJSAW010000000
VERSION     DAJSAW010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.194"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            7..624
                     /locus_tag="ONT64_002958"
     CDS             7..624
                     /locus_tag="ONT64_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304295.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          488 a          167 c          205 g          436 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt acttta
//
LOCUS       DAJSAW010000180         1291 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJSAW010000180 DAJSAW010000000
VERSION     DAJSAW010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.195"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>1291
                     /locus_tag="ONT64_002959"
     CDS             <1..>1291
                     /locus_tag="ONT64_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR2304296.1"
                     /translation="LFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKE
                     HLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSS
                     FKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNW
                     HIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPI
                     VYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPK
                     GKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDP
                     VAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEG
                     ARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVL"
BASE COUNT          428 a          230 c          279 g          354 t
ORIGIN      
        1 ctttttagtg atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca
       61 gtaaaaggac atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata
      121 acagagaaag aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag
      181 atgaaagata tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg
      241 gattgtctca cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt
      301 tcttctttta aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat
      361 agtttagtgg cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa
      421 gagaactatt ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg
      481 catattaaag cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt
      541 aatcaagaag agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa
      601 acagatacct attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat
      661 tatcaccacg acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca
      721 gggtatttac actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc
      781 tattgtttcg cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac
      841 acggatattc ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag
      901 aaatggaaag acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca
      961 ttatttattg ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg
     1021 gatgcagccg ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat
     1081 ggtcgtttag aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga
     1141 cggaaaaatt ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg
     1201 agtgtatata aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta
     1261 tttgataaga taccaaatct tcccgtactt t
//
LOCUS       DAJSAW010000181         1278 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJSAW010000181 DAJSAW010000000
VERSION     DAJSAW010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.196"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(305..661)
                     /locus_tag="ONT64_002960"
     CDS             complement(305..661)
                     /locus_tag="ONT64_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HCR2304297.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(675..1085)
                     /gene="spx"
                     /locus_tag="ONT64_002961"
     CDS             complement(675..1085)
                     /gene="spx"
                     /locus_tag="ONT64_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCR2304298.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          415 a          229 c          179 g          455 t
ORIGIN      
        1 actcagttat cttatcatgt catttctaac atgtcaacaa ctattttata tgttttttaa
       61 aatactattt tattgctttg ctacttaaaa caacagaaat gaatatatca tgtaaattta
      121 cacatgtcaa tatattttta actctatatc cccacctaga tttgaatttt gaccacataa
      181 attgagcatg tctctatagt agaaaaataa agtcaaaaaa agaacacata acacgcttga
      241 tgctaagtgt tcgactgtta caactgtatg aaatgctcag tgacttgatt aaaacagtta
      301 aagtctattt atttaagata agctgttcca tcaactgctc attgtatcca ataaatagaa
      361 cttcatcgat aataatcaaa ggacgtctga ggagattggt atgttgtgtg cataaaaaaa
      421 tcagttgttt ttttcctaca caggaaaggt caatcgcaat gttgtgaaaa gcctcagacc
      481 aagtcgcaaa tagctgttct gcttgcactt cttcaaaact gcataattct tctgcttctt
      541 ctgtacttag atcgcgttct tctaagtcaa caaattgata aggcaattga tggtgttcta
      601 accattgttt cgccttcaga cttgttacac agtgcgctga tccatagact ttcacttcca
      661 cgaaggctcc ctccctatga atttaagatg ttattgtttt gtaaatagat cagatttctg
      721 gcaacacatc gaatttcacg agggacaaat gaccggatct catcttcatt aaatccgatt
      781 agtattcttt tttcatcaat caaaataggt cgtctcaata atgaaggata ttctttgaat
      841 aagacgatca tttgctttaa agttaattcg ttaaaatcta tgcccagctt ttcgtaaatt
      901 ttcgaccgag tggaaataat atcttgaact cctccttcag tcgctgccaa gatctttttg
      961 atctcacttt ctgttaacgg ttcggcaaaa atattcttct cttcaaattc aagttgattc
     1021 gcttgcaacc atgctcttgc ttttctacag gaagtacagc taggcgaagt atatactttt
     1081 atcattttct cactcatttt taattataat ccaattctac agtaatttct tttaaaatac
     1141 caattattag ctaataatat ataaaaaaaa atcaaaataa ctgattattt acgtttgaat
     1201 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1261 taagggaggt gtgacaaa
//
LOCUS       DAJSAW010000182         1265 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJSAW010000182 DAJSAW010000000
VERSION     DAJSAW010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.197"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            137..1009
                     /locus_tag="ONT64_002962"
     CDS             137..1009
                     /locus_tag="ONT64_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2304299.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          394 a          230 c          286 g          355 t
ORIGIN      
        1 ttaaaaaatg ataaaaattc aaaaagaaaa aatagtagtt atttacttgt catgttacta
       61 aataaaaaaa taatttaaat atgatatata gtcatttatt tagtgatttc attctaaaat
      121 aaagggagaa atgtctatgc tatacggtgc aattgaagca ggcggcacaa aatttgtgtg
      181 tgccattggc gatgaagaaa tgacgatcaa ggaaagagtg agctttccga caacgacacc
      241 tgaggaaaca atgcctttag tcattgactt cttcaaacaa taccaagcag atttagcagg
      301 gattgggatt ggctcatttg ggcctatcga tatccatcgc gattccgcca cttatgggta
      361 cattacctcg accccgaaat tggcatggca aaattttgat ttcatcggta ccatgaaaaa
      421 agaatttccg atcccgattt cttggacaac agatgtcaat gcggctgcct atggtgagta
      481 tgtcttcggc agtggcaaag gattgagcag tgtcgtttac tatacgatcg gtacgggtgt
      541 tggcggcggt gccttacaag aagggcgctt tatcgaaggg tttagtcatc cagaaatggg
      601 gcatatgctc gtcgtccctc atccaaaaga tgactttgct ggcagctgtc catttcatgg
      661 caactgttta gaagggatgg ccgctggacc cgccatcgaa aaacgcttag ggaaaaaagg
      721 acaagaggtt gcagaagatg atccttattg ggaaatcgaa gcctcctata ttgctcaatg
      781 tgcctacaat acaaccttga tgttttctcc agatgtgatc atttttggcg gtggtgtgat
      841 gaaacaacgc catatgctgc aaaacgtcca cgacgcattt gcaaaattgg tcaatgggta
      901 tgtcgaaaca cctgagttaa gcaaatatat cgtgacccca gcattagaag acaacgctgg
      961 aacgctcggt tgtttggcat tggctcgaga agcggtctta cattcataaa cgaaaaaagg
     1021 ctcttcgaca tttagtgttg gtagagaaag atacaactga aaatttagtc ataaccagtt
     1081 aggcagagtg cttaactggt ttttgtttta cttggaagat caagccttga atgagataaa
     1141 ttcttgctcg aaagttatgg aaatttcgat agccgtaggc cacttgtatt gtgcaatagt
     1201 tttttacacg ccgtttgtat aattaagcga gacaaataaa aaagccattt atgttacact
     1261 caaat
//
LOCUS       DAJSAW010000183         1246 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJSAW010000183 DAJSAW010000000
VERSION     DAJSAW010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.198"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            200..742
                     /locus_tag="ONT64_002963"
     CDS             200..742
                     /locus_tag="ONT64_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304300.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="ONT64_002964"
     CDS             898..>1246
                     /locus_tag="ONT64_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HCR2304301.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAJSAW010000184         1233 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJSAW010000184 DAJSAW010000000
VERSION     DAJSAW010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.199"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            358..444
                     /locus_tag="ONT64_002965"
     CDS             358..444
                     /locus_tag="ONT64_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCR2304302.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(565..>1233)
                     /locus_tag="ONT64_002966"
     CDS             complement(565..>1233)
                     /locus_tag="ONT64_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304303.1"
                     /translation="LLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPGVIFTLS
                     IVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCYEEMND
                     IFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEACYNL
                     FSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEWLDQ
                     QQQN"
BASE COUNT          348 a          244 c          205 g          436 t
ORIGIN      
        1 taaacaggtt cgcgcatttt tcaaaactgc caatccaaaa ccttgcttcc tacggtcgcc
       61 aaaaaggtgt gatcgctacg ctctcacatt gtactgcgca gtcgcctaaa ggctcctttg
      121 ctaaaaacat gttggtaaag aggcctttga gcggttctga atgcttatct aagccgtttt
      181 aagacagtct tggataaaaa tccttatcta gctttaaagc gccttacagg cggtcagggc
      241 aagccctaac aaccctaaaa taagcgatac ttaaaaacac cacacaagtt taatttttta
      301 agatataata acttgtcgcc tatccgacag tcttgcgact ggaaggaggt gtatctaatg
      361 tttgaattgt tcatttgccc ccttcttata gaagtttgtg tctctttatt cagttattgg
      421 ttgaataacc gaaacaagaa gtaaaatcaa taaaggcgac atcaacctga aaaaaagccc
      481 cagagaatcc gacctctctg gggctttcgt gtatctaatg tttgatcatt tgcatgttta
      541 gtttaacatg attgccgatc aaattcaatt ttgctgttgc tgatctaacc attccttgac
      601 actttttaga taatttaatg ccgtttcttt tttcgttgga ttttgtatgt tcgttgcttg
      661 ctgaagcagt gctttataac gttcgtaggt aactgattgt tgccgttctt ttcgttcctg
      721 ttcactagtt gaaaaaggga ttgtatcact gaataaatta taacaagctt cttctgtctg
      781 cgtatcttta aaatattgca cgtctttttt ggttcgctca tagttgtctt gaacctcttt
      841 ttgctgtttt tctggcaagt tttgataaag acgagtgagt ttttccatgt ttttatccgt
      901 caaacgtttt tcttgattca caaaaatatc gttcatttcc tcataacaag tggtaaactc
      961 ttccattttt tgtgttaaca cttgctcctt tttcttttta tcacatgctg cttcatactt
     1021 ttgagtgata tcccctaata aaatacttct tgaaatacgt ttatttccct ctaaaacgcc
     1081 gaataaacca cttaaaacga ttgataatgt aaatataacc cctggcaata aaaacctctt
     1141 aaatcgaatt ttagagcctt ttaatgaata tcttgtttga taagccttat agctaagcca
     1201 aataattcct aaaatactgg caaaaatgag tag
//
LOCUS       DAJSAW010000185         1214 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJSAW010000185 DAJSAW010000000
VERSION     DAJSAW010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.200"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            70..1026
                     /locus_tag="ONT64_002967"
     CDS             70..1026
                     /locus_tag="ONT64_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304304.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1080..>1214)
                     /locus_tag="ONT64_002968"
     CDS             complement(1080..>1214)
                     /locus_tag="ONT64_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2304305.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          427 a          179 c          207 g          401 t
ORIGIN      
        1 ttgacaggga atcctaaaaa cattcggctt tttttatata atctttttaa ctcaacagag
       61 ataagttttg tggaattgta tacgctacct ataattataa aaacagaaaa aatgttaaaa
      121 gatttaaaac agtattatcc tctgtctgct tatgaaaaaa agaaattatt ccacttttta
      181 gcaatcaacc tgttggctga acaagctgat tctttagata ttgctagcaa tcagtcgcta
      241 tttttttcgg atataggatt ttaccactta aaaggagtat taggagaaaa atccgttatt
      301 tttatcaata agacactctc ttggctttat cttcatgata aattggatca aaaatacttg
      361 agaaaaaatg aagatgaaac aatcgaatgg ttgaatgaac gattcattga aagttttgaa
      421 aatcagttta atctgttgcg agaagagacg agccaattga tccgaagaga gttaaccaaa
      481 atacatttta aacttttgca ttacactatt aataaagctg atgaatttcc acttgatgtt
      541 tcttttttta gagaaaacta tccagcattt tattgctatt tgatagagta tataaatgat
      601 ctaacgtcaa aacgcaaaga gttgcttcaa gataaatttt ttcttttttt cagttattta
      661 ttattattga tcaatcacat acccattcaa ctgatttctg aaccagtaaa gatcatcatc
      721 gatttttctt atggtgctgc ctataatcaa tttatcaaga aaaatttaag cttgtacatt
      781 aatctgaaca cggaagtgat agaggcagga aagcctgagt tcccggatgt tataatcacg
      841 aataggaata atctatatga agaaagtgca tctcaagtag tggtttggct cgatccgcca
      901 agagcagtag actgggggaa tcttacaaag attttgttga ctatccaaga agagaaatac
      961 aaagacagca aaattgacaa gcaaatagac gattttcctg aagaaatcat cgacaggatc
     1021 gaatagtgaa cttcttttag aaagtccgac tgctttttct aaaaatatta tttgtcaaat
     1081 taagcgagac aaaactttcc atttacgtaa ctcaaaaaaa cttctaatgg tgtttggtaa
     1141 tttagtgatt ttctagggat atggtttctt ttggacgcaa cggatgagat gaatccttga
     1201 ttgacttggt tgaa
//
LOCUS       DAJSAW010000186         1195 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJSAW010000186 DAJSAW010000000
VERSION     DAJSAW010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.201"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1076
                     /locus_tag="ONT64_002969"
     CDS             <1..1076
                     /locus_tag="ONT64_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR2304306.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAJSAW010000187         1143 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJSAW010000187 DAJSAW010000000
VERSION     DAJSAW010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.202"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="ONT64_002970"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="ONT64_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR2304307.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DAJSAW010000188         1128 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJSAW010000188 DAJSAW010000000
VERSION     DAJSAW010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.203"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(27..1085)
                     /locus_tag="ONT64_002971"
     CDS             complement(27..1085)
                     /locus_tag="ONT64_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR2304308.1"
                     /translation="MVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGF
                     IEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSS
                     SRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAK
                     DFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVI
                     RPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAF
                     ICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISI
                     VFWYYLGSISYLYLKKEN"
BASE COUNT          435 a          192 c          138 g          363 t
ORIGIN      
        1 tccaagcaat gaaagctgtc ttcatattaa ttctcctttt ttaaatacaa gtaagaaatg
       61 cttcccaaat aataccagaa aacaattgaa atgacatcgc gcttattgaa aagtacatga
      121 gcctccacca tttcatctat aaaaatagat agtataaaaa ttaaaacaat accaagaaac
      181 gcataattca ctttacttct aaaaaatgtt ttgagtaaac taaagatatt taacacatag
      241 aaagcataga tacatataaa agcaattata ccacttgata caagtacttg aagaaatcca
      301 ttatgcatgt ttccatgtgc tctttttagt tcattcacat ctgtagtatc aagatctact
      361 tttttcaaaa attttgcatc gctattccca tagaaatcgg tctctccata accaaataac
      421 aaagttggtc ttataacttt aagcgcagct ttccaaatcg taaaacgacc agctgaaatt
      481 tcggagttat cactagaatg ctgtaatatt acttcttttt ctggaagtgg agttttatct
      541 gattcatttg actctataac ttctactttt ctatcaatcg atacaggaat tgttgctaaa
      601 aaatccttag cataattagt accaaaagaa aaatatacca atagaactaa taatatgctt
      661 accatccatt tgatttttac aggtagtact tctctaatgc tctttctaaa ttttgaattg
      721 gcaaacaaca aaagaaataa tactaaaaaa gaacaaatta ctacctgggc acctctagac
      781 gaagaaagtg taatataaaa tatttgtacg ataaaattca tcatataaaa aacacgaatc
      841 gtttttgtta tttttgaaat taatacacta attaaactta aaataataga agtatatcca
      901 aacatagctc caacatttgg acttgtatag acaccaaata accgatgttc aataaaacct
      961 tgtcttgcaa gtaatcctgg cgttcctgtt gggacatgat aagaaatcaa aaaaataaat
     1021 tgatagatag atattagcgc agctaaaaaa attatgataa tgaaaacatt attaaaatac
     1081 accatttttt tctttttttc ctcttcactt tgtgaaaagt ctgcagga
//
LOCUS       DAJSAW010000189         1051 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJSAW010000189 DAJSAW010000000
VERSION     DAJSAW010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.204"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ONT64_002972"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ONT64_002972"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCR2304309.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DAJSAW010000190         1048 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJSAW010000190 DAJSAW010000000
VERSION     DAJSAW010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.205"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            219..>1048
                     /locus_tag="ONT64_002973"
     CDS             219..>1048
                     /locus_tag="ONT64_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR2304310.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          372 a          150 c          178 g          348 t
ORIGIN      
        1 tttatagcaa agaaagttta ataagagttt ggacctagca aacaatatta atgaggtcat
       61 taaatcttat tatctttagt gatataacga aatatctaca cagacaaaat gatagcgtaa
      121 aatattttgt gtaaatagat atttagggta gaaaaaaaga aagtccattc tgtaaaatta
      181 aagttaccac aaccgaaatt ttataggagg actttctcat gcccaatttt actacagaaa
      241 ttatggaaac actaatcaat aaaggtgatt tagatgaatt atttcgatgt catttagaat
      301 tagctgttaa ttcattattg caggctgaat taacagcttt tcttgattat gaaaaatatg
      361 atcgtgctgg gttcaattca ggtaattccc gtaatgggaa ttattcacgt tcattcagaa
      421 cagagtatgg agaattaaat ttggtaattc ctagagatag aaatggtaag ttttctcaac
      481 aaactttacc tgcttataaa agaaccaatg actctttaga aaccactatt attcagctat
      541 ttcaaaaagg aattactatg tctgaaatct ctgaattgat tgaaaaaatg tacgggcatc
      601 actatacgcc acaaaccatt tcaaatatga ctaaaatagt ttctgaagat attattgcct
      661 ttaaagaaag gtccttagaa tccagatact ctgtcatttt tatggatgct actcacattc
      721 ctttaaaaag acaaactgtt tcaaaagaag cagtttatat tgtaattggc attcgattag
      781 atggaaccaa agaggttctt gggtttagtc ttgctccaac tgaatctgct tatgtttgga
      841 aagagattct tcaagaccta aaagaccgtg gtttaaaaga ggttttatta gtcgtaactg
      901 atggtttaag tggcattaac gatagtattc atagtgttta tccaaatgct caatttcaac
      961 aatgttgtgt ccatatatct agaaatatcg ctcataaggt tcgtgttagt gatcgacaag
     1021 aaatctgtag cgatttcaaa ttagttta
//
LOCUS       DAJSAW010000191         1004 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJSAW010000191 DAJSAW010000000
VERSION     DAJSAW010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.206"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            371..1004
                     /locus_tag="ONT64_002974"
                     /pseudo
     CDS             371..1004
                     /locus_tag="ONT64_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          184 g          296 t
ORIGIN      
        1 tgtttcaaag ttagtagaga aaatgtagtt atatatttaa atttttacca gaaagaaatg
       61 aacaccttca attatctaaa tctcgaaaat ggttagatgt aatcatcagg aaaaacaatc
      121 ttaaacgaat tactattcat ggtcttagac acacacatgc tagcttgctt ttagcaagct
      181 agtgcaaata ttaaagatgt gcaagagcgt ttaggccact cttctattca aatcattatg
      241 gatctatata ttcatattac agacaaaaga aaagaagaaa ccgtagcgca attcgcaaaa
      301 tataggctct ataatttatg ggactgatga atcaaaattg attcttcagt cccattgtta
      361 ttttaggtat atgattcgta cattgaaaga gttgaaaaac tatcttccga agccgtcaaa
      421 aaggaccctg ccaaaagtat tgatggtgga tgaatttcgt tcgcacgctt caattgtgga
      481 taaaatgagc tttatttgtg cggatggcga gactggaaaa ctcattgata ttttgcctac
      541 acgaaaattg tctcgattaa cacactattt tagtcgctgt gcaaatcctg aagcggtgga
      601 atatttagtg acagatatga acgccgccta cttccaactg acaaaacgtg tcttaccaaa
      661 tgcgaaagtc gtcattgatt gttttcatat catcaaacat ttgaatcagg cgttcaatga
      721 ttttcgtgta caagaaatga aaagattaca ccagttaggg caacgacggg aagccgagaa
      781 agtgaaaaag aactggcgct ttcttctaaa aaaccgtcaa aaaatcaatc actatgatta
      841 caaaacatgg cgcagttttc gtgcacctaa atacccgcta ctcactgaag caatgatgat
      901 tgatcgattg ctggcatttt caacgccttt aaaggacgcc tatacttttt tccatgagct
      961 gaccgaagcc tttagagaca aggaatcaga cttatttttt acct
//
LOCUS       DAJSAW010000192          997 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJSAW010000192 DAJSAW010000000
VERSION     DAJSAW010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.207"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(26..913)
                     /locus_tag="ONT64_002975"
     CDS             complement(26..913)
                     /locus_tag="ONT64_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCR2304311.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          323 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaatt
//
LOCUS       DAJSAW010000193          994 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJSAW010000193 DAJSAW010000000
VERSION     DAJSAW010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.208"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..>994)
                     /locus_tag="ONT64_002976"
     rRNA            complement(<1..>994)
                     /locus_tag="ONT64_002976"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DAJSAW010000194          986 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJSAW010000194 DAJSAW010000000
VERSION     DAJSAW010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.209"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..704
                     /locus_tag="ONT64_002977"
     CDS             <1..704
                     /locus_tag="ONT64_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR2304312.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          333 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataat
//
LOCUS       DAJSAW010000195          980 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJSAW010000195 DAJSAW010000000
VERSION     DAJSAW010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.210"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            82..>980
                     /locus_tag="ONT64_002978"
     CDS             82..>980
                     /locus_tag="ONT64_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HCR2304313.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYE"
BASE COUNT          334 a          182 c          192 g          272 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata
//
LOCUS       DAJSAW010000196          966 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJSAW010000196 DAJSAW010000000
VERSION     DAJSAW010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.211"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            5..652
                     /locus_tag="ONT64_002979"
     CDS             5..652
                     /locus_tag="ONT64_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR2304314.1"
                     /translation="MLVIFSGKQYADNILLWGGEDNIEKINNNLTILDTALENKEQKI
                     SILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKTNLENQLSAKDSELQG
                     KQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQSTINSLNQQINQMLSEK
                     NNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTANKAQQIVNEHTIQ"
BASE COUNT          425 a          117 c          139 g          285 t
ORIGIN      
        1 attgatgtta gtaattttct ccggaaagca atatgctgat aatattttat tatggggagg
       61 ggaagataat attgaaaaga tcaacaacaa tttaactata ttagacacag ctctagaaaa
      121 taaagagcaa aaaattagta ttttaaattc acaattgtct tcaaacaacc aaactcttga
      181 acaactaaga actaatattg aagtttccaa acaaaaagtg gcatctttag aaaacgacaa
      241 gaatcaatta gtatcagaga aaactaattt agaaaatcaa ttgtcagcaa aagactctga
      301 attgcaagga aaacaaaatg aaattaatgc gaaaatagac gaaattaacc aaaaagtagc
      361 tgaaataaat cgattaaata gcgattggaa taatcgttta caaaatgaaa agaacaaatt
      421 taatgaagaa caatctacta ttaatagttt aaaccagcaa attaatcaaa tgttgagtga
      481 aaaaaataac attcaaaatc aactgcaagc tactcagcaa aagctagaag aagaaaagtc
      541 aaaaaatagt agtttgaata attacattga aaagcttcaa aaagctaaaa atgatgtaga
      601 ggataccgca aataaagcac aacaaattgt aaatgaacat acaatccaat agatatagtt
      661 ttaatttaac aaatggtatg tttggagata atgagataga atataaatgt cataatatta
      721 tacaatctaa acaccgtttc cttttctatt tttgatgaca tcagtatata gctactcgaa
      781 agagtagtct ttttattatt ctcgcttatt attgatatga aaatttcgat ttgacaaaag
      841 gtaccgaata attttgtaat atgaattatt atcaagaagt aattatgatg ataacatatg
      901 gaaactagtt agtaataata acaggtgatt aaacttttga acgacttgta attctatatt
      961 ttttct
//
LOCUS       DAJSAW010000197          954 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJSAW010000197 DAJSAW010000000
VERSION     DAJSAW010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.212"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            70..>954
                     /locus_tag="ONT64_002980"
     rRNA            70..>954
                     /locus_tag="ONT64_002980"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          291 a          187 c          275 g          201 t
ORIGIN      
        1 attgcttatt ttcttgatct aacttctatc gctagaagaa ggatcaaaac ccaaccgtaa
       61 ggttgataag gttaagtgaa taagggcgca cggtggatgc cttggcacta ggagccgatg
      121 aaggacggga ctaacaccga tatgctttgg ggagctgtac gtaagctatg atccagagat
      181 ttccgaatgg gggaacccaa catctttaat aggatgttac gattgtgtga atacatagca
      241 cattcgaggt agacgcagag aactgaaaca tctaagtacc tgcaggaaga gaaagaaaat
      301 tcgattccct gagtagcggc gagcgaaacg ggaaaagccc aaaccagcaa gcttgcttgt
      361 tggggttgta ggactccaat atggtagttc tttcagatag tcgaatgact tggaaaagtc
      421 agtcaaagag ggtaaaaacc ccgtagacga aatgtggaag acacctagga ggatcctgag
      481 tacggcggaa cacgagaaat tccgtcggaa tccgggagga ccatctccca aggctaaata
      541 ctccctagtg accgatagtg aaccagtacc gtgagggaaa ggtgaaaagc accccggaag
      601 gggagtgaaa tagaacctga aaccgtgtgc ctacaacaag tcaaagcccg ttaatgggtg
      661 atggcgtgcc ttttgtagaa tgaaccggcg agttacgatt gcatgcgagg ttaagttgaa
      721 gagacggagc cgcagcgaaa gcgagtctga atagggcgtt tgagtatgta gtcgtagacc
      781 cgaaaccatg tgatctaccc atgtccaggt tgaaggtgcg gtaaaacgca ctggaggacc
      841 gaacccacgt acgttgaaaa gtgcggggat gaggtgtggg tagcggagaa attccaaacg
      901 aacttggaga tagctggttc tctccgaaat agctttaggg ctagcctcgg aatt
//
LOCUS       DAJSAW010000198          933 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJSAW010000198 DAJSAW010000000
VERSION     DAJSAW010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.213"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>933
                     /locus_tag="ONT64_002981"
     CDS             <1..>933
                     /locus_tag="ONT64_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HCR2304315.1"
                     /translation="ETFQKKKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKK
                     ETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLT
                     EKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMS
                     FICADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNA
                     KVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYE
                     YKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY"
BASE COUNT          303 a          178 c          187 g          265 t
ORIGIN      
        1 gagacctttc agaagaaaaa gacgttgatc gtcgatgctg ttttctcacc tgctccccat
       61 acctgtagaa attgtggttc tactgtggta gatggaaacg ggaaagtaat tgtagtgaaa
      121 aatgggaaga aggaaacgat tgtccgcttt gaacaataca atcacatgcc tttggttatg
      181 cgcttaaaaa agcagcgcta tacctgtaaa aactgtcgaa cccattggac ggctcaaagt
      241 tattttgttc aacccagaca ttcaatcgcg aatcatgtca gatataaaat tgcttcttta
      301 ctgactgaaa aagtatcttt atcttttatt gcgaaaaatt gtcaggtatc tttgaccacc
      361 gttattcgta cgttgaaaga gtttaaaagc tacttaccaa agcaatctaa gaggattctc
      421 cccagagtat tgatggttga tgaatttcgt tcgcacgctt ccatagaaga taaaatgagc
      481 tttatttgcg cagatggcga aacaggacaa ttaattgatg tcttgcctac gcgtaaattg
      541 cctcgattaa caagctattt cttaggctgc gctaatcctg aagaagtaaa atttttagtc
      601 acagacatga acgccgccta cttccagctt accaaacgta ttctgccaaa tgcgaaagta
      661 gtgattgatc ggtttcatat tatcaaacac atgaatcaag cctttaatga gttgcgtatc
      721 cgtgaaatga atgaacttcg taaagtagga cagaaaagtc aggcagaaaa actgaaaaag
      781 aactggcgct ttttgctaaa aaatcgtgca aacatcaacc attatgaata caaaacatgg
      841 aaaagttttc gagcaccaaa atacccattt cttacggaag cgatgatgat tgatcgattg
      901 cttggctttt ctacgtccct gaaggaggcg tat
//
LOCUS       DAJSAW010000199          883 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJSAW010000199 DAJSAW010000000
VERSION     DAJSAW010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.214"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            1..487
                     /locus_tag="ONT64_002982"
                     /pseudo
     CDS             1..487
                     /locus_tag="ONT64_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            545..>883
                     /locus_tag="ONT64_002983"
     CDS             545..>883
                     /locus_tag="ONT64_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304316.1"
                     /translation="MIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIG
                     LTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRF
                     MIFSTLFISFS"
BASE COUNT          281 a          124 c          119 g          359 t
ORIGIN      
        1 tattaatttt gaaacgttta gatcatatgg catgcaagta aataattttt acccatggtt
       61 aactctatat cctttatttt tattgatcaa gtttactaat ttagcaatag gctataacct
      121 ttttctttat attgttacgc tcattactct atttatatgt cattatgtaa tgtatgaaat
      181 taccaaaaaa catgttacta gctccttttt cgctattata tatacaacat cttcttttag
      241 gagtgtagaa atatttcttc gaggggcaat gggggaatta ctcgcaatgt caatacttcc
      301 tcttatttta ctcgggttta taaagcttta cgactcaaaa aaagaatctt gggttatgtt
      361 ggcgattagc atgactttat taatttacac tcatgttctt tctgtagcta tgactatggt
      421 aatgattata attgctttaa ttattcaatt ttatcggaaa aagaaaatag aaatcttctt
      481 aattattaaa ttaataaaag ctgctatagt tactttactt ctttcactag cgtttatagg
      541 tcctatgatt gagcagacat tgtatcaaga tctgaataga ccttatgtgg atatattaca
      601 aaaaagagca atttatctgg gtaaagagtt tttgattgga agtatagatt cagaattact
      661 atcatttggt attggcttaa ctttgcttat ttgtttaatt atactaacat ttaactttaa
      721 aaaattagga atcttatcta aaatcactta tataatgggt ataatatcta ttctactttg
      781 tacaaaaata tttccatggt ttgccttaca aaatactcct ttaaatatta ttcaagctcc
      841 ttggcgtttt atgatatttt caacattgtt catatctttt tca
//
LOCUS       DAJSAW010000200          878 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJSAW010000200 DAJSAW010000000
VERSION     DAJSAW010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.215"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            454..852
                     /gene="spxA"
                     /locus_tag="ONT64_002984"
     CDS             454..852
                     /gene="spxA"
                     /locus_tag="ONT64_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCR2304317.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          331 a          115 c          149 g          283 t
ORIGIN      
        1 atttgattta tttgtttata gtaaatttta tttagtaatc attgctttaa aatactacgc
       61 ttatataaca gagaaaataa gcgttataat aaagaacagt attatttaaa atgacagaaa
      121 acattactat taaagaaata aaaaacataa aaaatcttaa gaaatcggtt tcatttagct
      181 tttgagataa aataatagta cagttttata cgctgtttca tatgtttaga aaatttgaat
      241 tagatttgaa aactctaatt tttaaatgat aaaaaaagcg agaatgctgg ttttactgta
      301 tattaacctt agataacttt cattttttta aaaaaattat gaaaaaaagg tagctattta
      361 aaagttgatt atgtataata gaaacagatg ggagattatt atgatcttct gttacaaaaa
      421 agtgattctt atcacgaaaa ggagtggtgc acgttgttaa cattatatac ttccccaagt
      481 tgtacttcct gcagaaaggc tcgtgcgtgg ttgcaagaac atcagattcc atttgttgaa
      541 cgtaatattt tttcagaacc attgaacagt tctgaattaa aagcgatttt acaaatgaca
      601 gaagacggaa cagaagaaat catctcgaca cgttccaaag tattccaaaa actgaacatg
      661 gatttagatg atctgccgtt acaagaatta ttggaacttg tgcaaaacaa tcctggattg
      721 cttcgccgtc caatcatgat tgatgataaa cgtctgcaag ttggttttaa tgaagatgaa
      781 attcgtcggt tcctaccgcg cgatgtacga caattagaat tacgtcaagc acaattgatg
      841 gctggattat agaaggataa tacgaaactt ggaaaaga
//
LOCUS       DAJSAW010000201          875 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJSAW010000201 DAJSAW010000000
VERSION     DAJSAW010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.216"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(245..>875)
                     /locus_tag="ONT64_002985"
     CDS             complement(245..>875)
                     /locus_tag="ONT64_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCR2304318.1"
                     /translation="KNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTA
                     FKIFRAIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLN
                     FKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDP
                     SMNKWFDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
BASE COUNT          305 a          138 c           99 g          333 t
ORIGIN      
        1 gattatagtt aagataaaac taatcattgc tccaatcagt agccaagaaa aacgcatttt
       61 aaaaaaattt ttctttataa aaacctcata caaaactata attccacccc aagcaaaagc
      121 tattttcatc gggatattag ttacattaaa agtactaggc ataaaataaa gcagacaaat
      181 gattaaattt attactcgaa acgcatctcg acttaataaa ctcttctcta aaacaggtac
      241 tattctattc atctatattt ccaactttca aaatttcttt atcaattcta tttaatagtt
      301 tatcagagta atccgattta acttttctta caaataattt ttcactttct tttatttgat
      361 ctatatccct ttcatcaaac catttgttca tggaaggatc aataatatga atatttgcta
      421 atttcgaagt ttgtaaagta gtttcttcaa tatttttcaa ttcaagtggt aaatctggtc
      481 ctccaataac atttttattt ttgaacgagg aattaaaaaa aagtgtatga aaaaattttt
      541 catctggggc aaacatacct attttaaaat tcaggtagtc ttgttgattt tgttcataca
      601 tttgtattaa ttctgttagg cattctttcg acaaagccca ccattgagaa ccatgataca
      661 aatcaaattt atggtgtcta atttttgggg gaagtttaat catcctcaga caacgattaa
      721 ttatagctct aaaaatttta aatgcagttg tgttattcct attaattcta ggataatcat
      781 gtttttgata tacatctata tgtttataat aaaattcttt atgtttaatt tgatctagat
      841 ctattccacg aataaactcc actgaactat tcttt
//
LOCUS       DAJSAW010000202          815 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJSAW010000202 DAJSAW010000000
VERSION     DAJSAW010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.217"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            533..>815
                     /locus_tag="ONT64_002986"
     CDS             533..>815
                     /locus_tag="ONT64_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCR2304319.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENI"
BASE COUNT          270 a          130 c          172 g          243 t
ORIGIN      
        1 ttagagcagg agaaaacctt attttatcgt caaaacgtct cacagcgtaa attagagcgt
       61 tctgaagatg agatcgagtg gtagttacga tgtgcagtca acggtaccgg aggcggtttg
      121 tgccgaggat aaggagttga cggctaaacc actaatccct gaagctgaca tttgctaaaa
      181 aatggcaagt ccattttttg gccaacctag cgttagcgaa gtttgggggc gcccttttca
      241 aagggtgcgg ttttattttt tggatatttg catagctaaa gagaagtgct attattgagt
      301 tatcaaataa ataaactgaa aaaagagcaa aaaaataaag tcgagcaacg ctggacacgt
      361 tctcgacctt tacggaaact aatcgcacta gctccgtaac ttcttaatca ttatacgaat
      421 atttttagat ttcttcaaga ctagagattt tttggtaatg aatttttaag agttacgtga
      481 acttttgaaa attctttcca gaagtctctt tttctttgag gaggaacaaa atttgagtaa
      541 aaaaggcacg atcataaatg agtgggaaaa attaacagcc ccctctaaca ggcggttaaa
      601 gtatcaaagt ttgacccttc ccttaaaaat gcagtggtca attgaccgaa ttaccatagt
      661 cggaaaactg aaagaaaata tttattatca cactccaaat gatgtgttga ttttaaattt
      721 tgaacagctt atgcgcctaa atgaaggtaa tggttattta agatctgtta gtaataatgg
      781 ttggcagttg ttagaccaac atgaagagaa tattg
//
LOCUS       DAJSAW010000203          811 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJSAW010000203 DAJSAW010000000
VERSION     DAJSAW010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.218"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..672
                     /locus_tag="ONT64_002987"
     CDS             <1..672
                     /locus_tag="ONT64_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2304320.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAJSAW010000204          764 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJSAW010000204 DAJSAW010000000
VERSION     DAJSAW010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.219"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(109..>764)
                     /locus_tag="ONT64_002988"
     CDS             complement(109..>764)
                     /locus_tag="ONT64_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HCR2304321.1"
                     /translation="GRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPL
                     EAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDV
                     KLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          213 a          175 c          146 g          230 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccga
//
LOCUS       DAJSAW010000205          736 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJSAW010000205 DAJSAW010000000
VERSION     DAJSAW010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.221"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..734
                     /locus_tag="ONT64_002989"
     CDS             <1..734
                     /locus_tag="ONT64_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HCR2304322.1"
                     /translation="DKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRK
                     LMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKI
                     YLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQ
                     YQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIR
                     WYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          318 a           94 c          122 g          202 t
ORIGIN      
        1 cagataaata tagcaagata aagcaagaaa ttacagcgat tgtaaaagaa tcaagaaact
       61 cttatggtta tcgtagagtc actttagcgt taaagatgaa aggatataca atcaatcata
      121 aaacagttag aaaattaatg tcccaaatgg gacttacctg tcaaatccga ataaagcgat
      181 ataaatctta taaaggaaca gtaggaaaaa ttgccaagaa tgtgttaaaa cgaaattttt
      241 cagtagatac acccaataaa aaatgggtga cagacgtcac tgaattcaag ataaaaggaa
      301 gaaaaatcta tctatctcct atacttgatt tatttaacgg agaaataatt agttatagta
      361 tatcaactag tccaacatat aaactgattg aagaaatgct tcaacaagca attaaaaaaa
      421 agggaactga gggttcactg attctacatt cagatcaagg atggcagtat cagatgccac
      481 aatatcaaaa aaaattaaaa gagaataaca ttattcaaag catgtctaga aaaggaaatt
      541 gcttagataa ttctgtaata gaaaattttt ttggtgtgct taaatcagag tttttttatc
      601 gagaaaaatt ccgatcaatt gagatatttc aaagtaaatt aaatgagtat attaggtggt
      661 ataataacaa aaggataaaa ctaaagttaa atggactatc tccagtagaa tatcggaaac
      721 agtccattaa atagtt
//
LOCUS       DAJSAW010000206          729 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJSAW010000206 DAJSAW010000000
VERSION     DAJSAW010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.222"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..729
                     /locus_tag="ONT64_002990"
     rRNA            <1..729
                     /locus_tag="ONT64_002990"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          180 a          162 c          229 g          158 t
ORIGIN      
        1 tgggatacta cccctgcgtt atggccactc taacccgcac cactaatcgt ggtgggagac
       61 agtgtcagat gggcagtttg actggggcgg tcgcctccta aaaggtaacg gaggcgccca
      121 aaggttccct cagaatggtt ggaaatcatt cgaagagtgt aaaggcagaa gggagcttga
      181 ctgcgagacc aacaagtcga gcagggacga aagtcgggct tagtgatccg gtggttccgc
      241 atggaagggc catcgctcaa cggataaaag ctaccctggg gataacaggc ttatctcccc
      301 caagagtcca catcgacggg gaggtttggc acctcgatgt cggctcgtcg catcctgggg
      361 ctgtagtcgg tcccaagggt tgggctgttc gcccattaaa gcggcacgcg agctgggttc
      421 agaacgtcgt gagacagttc ggtccctatc cgtcgcgggc gttggaaatt tgagaggagc
      481 tgtccttagt acgagaggac cgggatggac ttaccgctgg tgtaccagtt gttctgccaa
      541 gggcattgct gggtagctat gtagggaagg gataaacgct gaaagcatct aagtgtgaag
      601 cccacctcaa gatgagattt cccatttctt taagaaagta agatccctga gagatgatca
      661 ggtagatagg tcaggagtgg aagtacagtg atgtatggag cggactgata ctaatcgatc
      721 gaggactta
//
LOCUS       DAJSAW010000207          712 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJSAW010000207 DAJSAW010000000
VERSION     DAJSAW010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.223"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..446)
                     /locus_tag="ONT64_002991"
     CDS             complement(<1..446)
                     /locus_tag="ONT64_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2304323.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DAJSAW010000208          695 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJSAW010000208 DAJSAW010000000
VERSION     DAJSAW010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.224"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(53..628)
                     /locus_tag="ONT64_002992"
     CDS             complement(53..628)
                     /locus_tag="ONT64_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR2304324.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          229 a          151 c          124 g          191 t
ORIGIN      
        1 atgaaaaaac agagccagat tagcattatt ttgctaatcc agctctgttt attcagccta
       61 tttttctttt tgaatatatc aagcgttggg gcaacatagt gatgactaat gaaacaacga
      121 cgatagacaa caagcgatca aggtagtctg tacccgcctg taccaagatc acacttgttg
      181 tctgacttaa acctaaccca tgtaatagct gaacgatcat actggatcct gaggaggtga
      241 tcccgccaaa taactgaacg gtaatgatcg aagaaacgat cgttccaggt accgacaaaa
      301 tcaaagcgag taataacagt ttcaagctgt ttttatcttt gccgagatat cttggcaaaa
      361 tcaatccagc aagcaagcct gtgagaattt ggattggcga ataatacaga gaaaaaatat
      421 ctgtagtcac tccacttaaa ataccgctgg acaatcctgt cagcattcca ggtacaggcc
      481 caagcaatgc accagcaaaa atcgttccga tagaatcgag atagattggc aaacgaagcc
      541 ataaagcaat cgttcctcca atataattga tcccaataca caaagccatc actgtaagta
      601 cgcgagttgc cattttgttt ttattcattt tgttagccca tctttaacct ttccaaatct
      661 ttcataatcg tttcttcttg tgcatttaaa agaac
//
LOCUS       DAJSAW010000209          667 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJSAW010000209 DAJSAW010000000
VERSION     DAJSAW010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.225"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(107..511)
                     /locus_tag="ONT64_002993"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="ONT64_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
BASE COUNT          212 a          123 c          104 g          228 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctga
//
LOCUS       DAJSAW010000210          649 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJSAW010000210 DAJSAW010000000
VERSION     DAJSAW010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.226"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>649
                     /locus_tag="ONT64_002994"
     CDS             <1..>649
                     /locus_tag="ONT64_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2304325.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSV"
BASE COUNT          213 a          111 c          134 g          191 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtag
//
LOCUS       DAJSAW010000211          635 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJSAW010000211 DAJSAW010000000
VERSION     DAJSAW010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.227"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..270)
                     /locus_tag="ONT64_002995"
     CDS             complement(<1..270)
                     /locus_tag="ONT64_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304326.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="ONT64_002996"
     CDS             complement(276..>635)
                     /locus_tag="ONT64_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCR2304327.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DAJSAW010000212          634 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJSAW010000212 DAJSAW010000000
VERSION     DAJSAW010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.228"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(20..91)
                     /locus_tag="ONT64_002997"
     tRNA            complement(20..91)
                     /locus_tag="ONT64_002997"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ONT64_002998"
     tRNA            complement(113..200)
                     /locus_tag="ONT64_002998"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ONT64_002999"
     tRNA            complement(225..298)
                     /locus_tag="ONT64_002999"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ONT64_003000"
     tRNA            complement(312..382)
                     /locus_tag="ONT64_003000"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ONT64_003001"
     tRNA            complement(384..459)
                     /locus_tag="ONT64_003001"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="ONT64_003002"
     tRNA            complement(478..551)
                     /locus_tag="ONT64_003002"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
BASE COUNT          173 a          171 c          151 g          139 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagc
//
LOCUS       DAJSAW010000213          621 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJSAW010000213 DAJSAW010000000
VERSION     DAJSAW010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.229"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>621
                     /locus_tag="ONT64_003003"
     rRNA            <1..>621
                     /locus_tag="ONT64_003003"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          184 a          130 c          178 g          129 t
ORIGIN      
        1 aagtctgaag aggagtcaaa tgcttctttt cttaaggaca agctgtgatg gggagggaaa
       61 taatagtacc gaagttcctg atgtcacact gccgagaaaa gcttctagtg agaaaacagc
      121 ggcccgtacc gcaaaccgac acaggtagtc gaggagagaa tcctaaggtg agcgagagaa
      181 ctctcgttaa ggaactcggc aaaatgaccc cgtaacttcg ggagaagggg tgctgatcat
      241 acgatcagcc gcagtgaata ggcccaagcg actgtttatc aaaaacacag gtctctgcaa
      301 aatcgtaaga tgaagtatag gggctgacgc ctgcccggtg ctggaaggtt aagaggagtg
      361 cttagcgcaa gcgaaggtac gaattgaagc cccagtaaac ggcggccgta actataacgg
      421 tcctaaggta gcgaaattcc ttgtcgggta agttccgacc cgcacgaaag gcgtaacgat
      481 ttgggcactg tctcaacgag agactcggtg aaattttagt acctgtgaag atgcaggtta
      541 cccgcgacag gacggaaaga ccccatggag ctttactgta gtttgatatt gagtgtctgt
      601 accgcatgta caggataggt a
//
LOCUS       DAJSAW010000214          603 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJSAW010000214 DAJSAW010000000
VERSION     DAJSAW010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.230"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
BASE COUNT          199 a          118 c           84 g          202 t
ORIGIN      
        1 aaccgggggc tttattcctg cagtccggct acgccttcct gcaatagaca aaactccaga
       61 tcgggtaatt cgcttgtggc gaaacccgac ctggagtttg tctctgtatt ttgaacccta
      121 aaaattaatc aaacagactg atattctcat aatcagcgcg aaataagcca ttaagtacag
      181 ttttcctacc tatttttgat aacccgctgg ttagttaata ttccttttcc gagagagata
      241 aatgattggt taaatctata tacatctcaa cctttaaaaa tgttttcgaa atacttcatc
      301 agtacatgtc atacaaatta ccagtgcatt ctcaaattct agttctttaa ttgatttcag
      361 ttgaggagca gttacttttg gcggaaattt ccatttttgt ccaaacctct tcccccgatg
      421 aaatttcttt gttgcaatta ttgcaatgaa aaatttttgt ttcaaaaata tacataatta
      481 cttcctccaa taaaaactat ctacctctca aattattttt ctctaataag aaaaacagaa
      541 aaggtaataa gatacaacag tgatgattcc taataaagaa aagactacaa taatattttt
      601 cta
//
LOCUS       DAJSAW010000215          541 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJSAW010000215 DAJSAW010000000
VERSION     DAJSAW010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.231"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..156
                     /locus_tag="ONT64_003004"
     CDS             <1..156
                     /locus_tag="ONT64_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR2304328.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..>541
                     /locus_tag="ONT64_003005"
     CDS             175..>541
                     /locus_tag="ONT64_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2304329.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEAT"
BASE COUNT          236 a           45 c           97 g          163 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 a
//
LOCUS       DAJSAW010000216          528 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJSAW010000216 DAJSAW010000000
VERSION     DAJSAW010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.232"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(5..90)
                     /locus_tag="ONT64_003006"
     tRNA            complement(5..90)
                     /locus_tag="ONT64_003006"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="ONT64_003007"
     tRNA            complement(107..178)
                     /locus_tag="ONT64_003007"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="ONT64_003008"
     tRNA            complement(190..262)
                     /locus_tag="ONT64_003008"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="ONT64_003009"
     tRNA            complement(280..361)
                     /locus_tag="ONT64_003009"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="ONT64_003010"
     tRNA            complement(369..441)
                     /locus_tag="ONT64_003010"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="ONT64_003011"
     tRNA            complement(445..517)
                     /locus_tag="ONT64_003011"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAJSAW010000217          526 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJSAW010000217 DAJSAW010000000
VERSION     DAJSAW010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.233"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ONT64_003012"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ONT64_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR2304330.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(349..>526)
                     /locus_tag="ONT64_003013"
     CDS             complement(349..>526)
                     /locus_tag="ONT64_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304331.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DAJSAW010000218          524 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJSAW010000218 DAJSAW010000000
VERSION     DAJSAW010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.234"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            1..524
                     /locus_tag="ONT64_003014"
                     /pseudo
     CDS             1..524
                     /locus_tag="ONT64_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          218 a           91 c           95 g          120 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag aagcgatcgt aaca
//
LOCUS       DAJSAW010000219          509 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJSAW010000219 DAJSAW010000000
VERSION     DAJSAW010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.236"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
BASE COUNT          228 a           70 c           82 g          129 t
ORIGIN      
        1 gaattcctta ggcagctttt gctgtgtaga aaggaataaa taataacgaa agttcaaaag
       61 ctaatgccga ctattctaga cattaaagaa gacgaaaacg aacgataaag aaagaaaagt
      121 tatgaataat cgtttattga aaaaaacttg aaaaaataca aaaaattcgt tgaccaagct
      181 aaatttagtt ggtatattat tacttgtcca aaagacagaa gaaaatattt attcaaaaac
      241 ataacaaaaa agttattgac atgttactaa cgacatgata agataactga gtcgttaaaa
      301 aagaaccact tgcgaagctt gattctatcg aaaaaaactt caaaaaagtt cttgacaaat
      361 aacaaacgat aagttatgat aaatgagttg ccgaaagatt tcgaaaaacg aaatcgaaaa
      421 aaacttttaa aaaagagttg acaatcgctt gtctctctga tatgatataa aagttgctac
      481 ggcagccaaa aatgaatcgg gaaacactc
//
LOCUS       DAJSAW010000220          509 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJSAW010000220 DAJSAW010000000
VERSION     DAJSAW010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.237"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..132)
                     /locus_tag="ONT64_003015"
     CDS             complement(<1..132)
                     /locus_tag="ONT64_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR2304332.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DAJSAW010000221          493 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJSAW010000221 DAJSAW010000000
VERSION     DAJSAW010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.238"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..378
                     /locus_tag="ONT64_003016"
     CDS             <1..378
                     /locus_tag="ONT64_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HCR2304333.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          182 a           51 c           85 g          175 t
ORIGIN      
        1 ccaatagaat ttgacaatga tgaaagattg ctgctaatat atgcgtttat ggatgaaaat
       61 tctatctttt caactgataa ttcaacttat agaaaagtag ttcaatggga aagtgataac
      121 aatttaagga gttacttgag tgatagagta tttgatgtac tagaaaaatt aagtaaatcg
      181 ggaattttaa aggaagaata cgattcttct tttgatgagt atccatcacg ctatattttg
      241 atttcagagg actataagaa atttaaaagg tattttttaa aaaatgaaaa aaagatatct
      301 gttattatgg agaaatacaa agctccaata caggctgatc cattttccaa tttggatgaa
      361 tcagatttac cgttttaaat tttaaataga taatttcatt ttttagtagc agaggtataa
      421 acttttttaa tgtttgaatt gaaaagaaat gctaataggt cgactttatg tttctcttgt
      481 gcaatcgtaa atg
//
LOCUS       DAJSAW010000222          452 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJSAW010000222 DAJSAW010000000
VERSION     DAJSAW010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.239"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>452
                     /locus_tag="ONT64_003017"
     CDS             <1..>452
                     /locus_tag="ONT64_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304334.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DAJSAW010000223          448 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJSAW010000223 DAJSAW010000000
VERSION     DAJSAW010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.240"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..304
                     /locus_tag="ONT64_003018"
     CDS             <1..304
                     /locus_tag="ONT64_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2304335.1"
                     /translation="RFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIE
                     GFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          174 a           65 c           63 g          146 t
ORIGIN      
        1 aaggtttatg atagataaat ggaaaaagca gtatccacga gtggttaaat tgctcatgaa
       61 tcctgctata ttaacctttt ataatttccc tccgtcaatc agaagaacta tttattcaac
      121 taatttgatt gaaggtttta acaaacaatt aaagaaatat accaaaagaa aagagcaatt
      181 tcctaatgaa gaatctctgg agagatttct agtatctcaa ttcaacaact ataatcaaaa
      241 gtttctatgc cgagttcata aaggttttaa agaaatacat gatacattag aatcaatgtt
      301 ttaacttgat caacagaatg ggcttttcca tttacacaaa attattgacg ctcccgacaa
      361 aattatgatc ttgcgtaaag ttataaaata ttaataaaaa tattttatta atgaaaagct
      421 tggtagcacg gttattcata tgctaatt
//
LOCUS       DAJSAW010000224          440 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJSAW010000224 DAJSAW010000000
VERSION     DAJSAW010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.241"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            49..243
                     /locus_tag="ONT64_003019"
     CDS             49..243
                     /locus_tag="ONT64_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304336.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          189 a           57 c           80 g          114 t
ORIGIN      
        1 acatatattt gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta
       61 gaagtaaaaa caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat
      121 cctttacttg gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata
      181 aagaacgagc tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag
      241 taaatccaat gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat
      301 tatagaatta gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata
      361 ccatatgatt atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat
      421 gaatcaaata tacttgtcat
//
LOCUS       DAJSAW010000225          436 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJSAW010000225 DAJSAW010000000
VERSION     DAJSAW010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.242"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            227..424
                     /locus_tag="ONT64_003020"
     CDS             227..424
                     /locus_tag="ONT64_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2304337.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           61 c           78 g          139 t
ORIGIN      
        1 ttacgtaaat ggaaagtttt gtctcgctta atttgacaaa taataatatt taaaccgtat
       61 atcgagtgat tatccttgtg ttagcattac atccgtgtta tagtaaataa gtaatctaat
      121 ttgaaacgta atctgagcga tatattcaca ctataaaaac tccttttacc aagtaatatt
      181 aattgcaata aaacacgtat tatatacgta tcaggaggaa atatatatga ataacggtac
      241 agtaaaatgg tttaactcag ataaaggttt tggtttcatc actggtgaag atggaaatga
      301 cgtattcgct catttctcag caatccaagg agaaggcttt aagtctttag atgaaggcca
      361 agcagttact tatgatattg aagaaggaca acgtggtcct caagcagtaa atatcgtaaa
      421 ataatgttga acgtta
//
LOCUS       DAJSAW010000226          415 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJSAW010000226 DAJSAW010000000
VERSION     DAJSAW010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.243"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..339)
                     /locus_tag="ONT64_003021"
     CDS             complement(<1..339)
                     /locus_tag="ONT64_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2304338.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAJSAW010000227          409 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJSAW010000227 DAJSAW010000000
VERSION     DAJSAW010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.244"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..379
                     /locus_tag="ONT64_003022"
     CDS             <1..379
                     /locus_tag="ONT64_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HCR2304339.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKT
                     RKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          139 a           79 c           81 g          110 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatataa
      301 gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt
      361 attgggaatc ccagcttaaa tcatagatac cgtaagggat tttattctt
//
LOCUS       DAJSAW010000228          408 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJSAW010000228 DAJSAW010000000
VERSION     DAJSAW010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.245"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            124..327
                     /locus_tag="ONT64_003023"
     CDS             124..327
                     /locus_tag="ONT64_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HCR2304340.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
BASE COUNT          167 a           43 c           78 g          120 t
ORIGIN      
        1 tcaatcattg ttatataata aaatgttata taataaaatt actaatgata tttaatatta
       61 caagcaatct taagaaattc gaaaaggaaa aaatgattat gaaaaagaaa gaaaaagaaa
      121 gtattgaaac attgtgttat tctaggaata ttaggaactt gtctagctgg cattggtaca
      181 ggaatagacg ttgacgcagc tacttactat ggaaatggtc tttattgtaa caaagaaaaa
      241 tgttgggtaa attggggaca atcatggtca gaagggttaa aacgttgggg agataatctt
      301 tttggtagtt ttagtggagg acgataatga cttcaataaa cattgatcac acaaaaaaag
      361 tcatacatga attatacaat agtttaatga cacgacctaa tcagacga
//
LOCUS       DAJSAW010000229          404 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJSAW010000229 DAJSAW010000000
VERSION     DAJSAW010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.246"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>404
                     /locus_tag="ONT64_003024"
     CDS             <1..>404
                     /locus_tag="ONT64_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304341.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DAJSAW010000230          400 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJSAW010000230 DAJSAW010000000
VERSION     DAJSAW010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.247"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>400
                     /locus_tag="ONT64_003025"
     CDS             <1..>400
                     /locus_tag="ONT64_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
                     /protein_id="HCR2304342.1"
                     /translation="SGKLLEALELEQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVI
                     TNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEKIKIT
                     EMYTSINGELCKIDKAYSGEIVILQNEFLKL"
BASE COUNT          146 a           51 c           81 g          122 t
ORIGIN      
        1 gtctgggaaa ttattggaag cattagaact cgaacaagag gaaagcataa gatttcataa
       61 ttgttccctg ttccctgttt atcacggaag tgcaaaaaac aatataggga ttgataacct
      121 tatagaagtg attacgaata aattttattc atcaacacat cgaggtcagt ctgaactttg
      181 cggaaaagtt ttcaaaattg agtattcgga aaaaagacag cgtcttgcat atatacgtct
      241 ttatagtggc gtactgcatt tgcgagattc ggttagaata tcggaaaagg aaaaaataaa
      301 aattacagaa atgtatactt caataaatgg tgaattatgt aaaatcgata aggcttattc
      361 cggggaaatt gttattttgc aaaatgagtt tttgaagtta
//
LOCUS       DAJSAW010000231          381 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJSAW010000231 DAJSAW010000000
VERSION     DAJSAW010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.248"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(21..94)
                     /locus_tag="ONT64_003026"
     tRNA            complement(21..94)
                     /locus_tag="ONT64_003026"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="ONT64_003027"
     tRNA            complement(108..181)
                     /locus_tag="ONT64_003027"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="ONT64_003028"
     tRNA            complement(209..281)
                     /locus_tag="ONT64_003028"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="ONT64_003029"
     tRNA            complement(301..374)
                     /locus_tag="ONT64_003029"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DAJSAW010000232          343 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJSAW010000232 DAJSAW010000000
VERSION     DAJSAW010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.249"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
BASE COUNT          126 a           57 c           54 g          106 t
ORIGIN      
        1 attagcgatg cagggatcga gagaaacatc aaaagggtat ccttcaagat cacgttaaac
       61 aagcctaccg ataaaaagct ctcagaagcg ttctgagagc ttttgacatt ttcttgtata
      121 attttatcct aacacattac aaagcaccta attcgagctt atatcaagca aatttgctat
      181 ttattactca atacatatct aaaaattact tgatggaaaa ttagatacat ggtaagcatg
      241 aaaagaaaat caatttgagc agatgcagta caatacatag caatttaaaa gctctcagaa
      301 ggcttctgag agcttttaat atattctttg ataatttgtc ttt
//
LOCUS       DAJSAW010000233          341 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJSAW010000233 DAJSAW010000000
VERSION     DAJSAW010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.250"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>341
                     /locus_tag="ONT64_003030"
     CDS             <1..>341
                     /locus_tag="ONT64_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCR2304343.1"
                     /translation="RLPDGSYHLSGNDFVDLYQRATRATNAKRNADNMSEQYLQMLEQ
                     NTELNEYVATLEEERHLSGRKQLADLQVENKKLKEENRSLRQRLERLKNAFQKSITRL
                     SIRFGLAEKDMG"
BASE COUNT          120 a           50 c           76 g           95 t
ORIGIN      
        1 aggcttcctg atggttcgta tcatttatct gggaatgatt ttgttgattt gtatcaacga
       61 gctacgagag caacaaatgc caagagaaat gctgataaca tgagtgagca atatttacag
      121 atgcttgagc aaaatacaga attaaatgag tatgtagcta cattggaaga agagcgtcat
      181 ttgtctggta gaaaacaatt agcagattta caagtggaaa ataaaaagct gaaagaagaa
      241 aatcgctctt tacgccagcg tttagagcga ttaaaaaacg catttcaaaa atcgatcact
      301 cgtttgtcta tccgttttgg tcttgcagaa aaggatatgg g
//
LOCUS       DAJSAW010000234          339 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJSAW010000234 DAJSAW010000000
VERSION     DAJSAW010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.251"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..321)
                     /locus_tag="ONT64_003031"
     CDS             complement(<1..321)
                     /locus_tag="ONT64_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR2304344.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT           95 a           55 c           61 g          128 t
ORIGIN      
        1 taaatggata atagtggttt ccaatgtatc gtttgttctt ttgtaggctg gtaaagtttg
       61 ttgtttaaac tcaccattac gatctctagg tatttccaat gttaattcac catattcggt
      121 tttgattgat cgaaagtaag aaccgtttct cgaattacct gaattaaaac cagtgcgatc
      181 atatttttcg taatctaaaa aagccgttaa ttcagtccgt aggagtgtgt ttatcgcttt
      241 ttctaagtgc gaacggaata attcatttaa atcgccttta gtgactagag tttgcacaat
      301 ttctgtagta aaatcattca tagggaagtc ctcttttct
//
LOCUS       DAJSAW010000235          321 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJSAW010000235 DAJSAW010000000
VERSION     DAJSAW010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.denovo.252"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(69..321)
                     /locus_tag="ONT64_003032"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="ONT64_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAJSAW010000236         9288 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.guided.080, whole genome shotgun sequence.
ACCESSION   DAJSAW010000236 DAJSAW010000000
VERSION     DAJSAW010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.guided.080"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..392
                     /locus_tag="ONT64_003033"
     CDS             <1..392
                     /locus_tag="ONT64_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCR2304345.1"
                     /translation="PHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFES
                     MYFQKKLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAE
                     NWAKRFITNDSTIYEQYEYDLSSVENK"
     gene            complement(542..1483)
                     /locus_tag="ONT64_003034"
     CDS             complement(542..1483)
                     /locus_tag="ONT64_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR2304346.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1609..2730
                     /locus_tag="ONT64_003035"
     CDS             1609..2730
                     /locus_tag="ONT64_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR2304347.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            2988..4490
                     /gene="eat(A)"
                     /locus_tag="ONT64_003036"
     CDS             2988..4490
                     /gene="eat(A)"
                     /locus_tag="ONT64_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCR2304348.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4518..5084
                     /locus_tag="ONT64_003037"
     CDS             4518..5084
                     /locus_tag="ONT64_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR2304349.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            5295..6356
                     /locus_tag="ONT64_003038"
     CDS             5295..6356
                     /locus_tag="ONT64_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR2304350.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6359..7255
                     /gene="murQ"
                     /locus_tag="ONT64_003039"
     CDS             6359..7255
                     /gene="murQ"
                     /locus_tag="ONT64_003039"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR2304351.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7269..9251
                     /locus_tag="ONT64_003040"
     CDS             7269..9251
                     /locus_tag="ONT64_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2304352.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
BASE COUNT         3047 a         1657 c         2052 g         2532 t
ORIGIN      
        1 ttccacatcg agggaatgaa caacctggat atcttactta tgaagagtac ctgaaaaaaa
       61 caggggaatc gcgcgggatt ctggaactgt tgcgggaggg tcagcaagga gtgactttgc
      121 ggacatttga atccatgtat tttcaaaaga aactagtgac ggataatcaa gcgattgctc
      181 attatcattt ttatcgtcct gaaaatattt ttttgcttcg agaacgtgat ttagatgaat
      241 taccggaatt tctgcataca ccatttaagc caatcctatc agagctattg gaattttatg
      301 atgcagaaaa ttgggcaaaa cgatttatca ctaacgattc cactatttat gagcaatacg
      361 aatatgacct ctcctctgtt gaaaacaaat aaaaaaccta cattttgaag agattatgta
      421 ctgaaacaaa caagcacaat tcttatggca tcatgagagt tgtgttttct ttttgatctt
      481 ttattgggct taaaaaaaga gaaccagctg ctgattcagc tgattctcat cagagagcct
      541 tttattctgc gccaaggatt ccttcttttg catggactaa ttgggtcaaa aaggcacaat
      601 atggtgtagc aatatcatat ttttgtcctt ttcgccaaat ggcaccattg atgtagtcga
      661 tttcagtcaa acgatggttt ttgatcaggt cttggtacat agatgggtaa tgcaacccga
      721 tgttgtcagg atcataagta gaagcaatat gttggtacac ttcttcttga tctagtatga
      781 ttccttcttt tgctgcgata gaagcaaatt cagatacgat tgttcgaacc atgctctctg
      841 ccgctttttg cgcgcctagt tcagcaatat tacaatccaa aatcgtgcat aaaccattca
      901 atgttccatt tacacaagct tttcgccaaa tggaataacg aacgttatgg ctatagatcg
      961 gatttaaatt tgcttcttga aagacttcta caactttttt tgtaaatgct tcgccttctg
     1021 gttccaagtt ctctagttcg atttctccat cacctaacaa tgtgactttt ccgggaccag
     1081 ctagaccagc tgtccacatc gtgatcccaa ataagatatt tttctttggc acatattttt
     1141 ccagtacatc ttcatgacct aatccattta gtaaacacaa aacataagta ttgtcagtaa
     1201 taatggattg gatcgaacaa aacatatcat ctaattgatt ggcttttgtt aatgctacga
     1261 tcaagtccac gtgttcgttg ctttcgatga tttcttctgg tgaatagatt ggcagttttg
     1321 cgaccacttc tttaccgtta aagtcagcaa tcaacccatt tgtacgaatg gcttcaatgt
     1381 gagcaggcca acgatcgatc aagaggacct catttccgct ttgatgtagc atcaatccga
     1441 ttcggctgcc cattgctccg gccccagcta ttgcgatttt catgtttttt ctctccttta
     1501 ttcaatgagt tcccttttag tatatagaaa aatggaagaa aatagcattg ttttgtttga
     1561 aaaaaatgaa aaaaagtgtc ttacttaaga agaaagtgag ggatacagat ggatattttg
     1621 gcggcaattg attcatttaa aggaagtgct tcttcatttg aactaaatca ggcagttcta
     1681 aatggctggg aagatcgtaa aggaagaaaa atcaatgtgc cgattgcaga tggcggagaa
     1741 aatactgcag aagcaatcta ttacgcgctc aggggaacat ggcgtaccat tgatgggatg
     1801 gatctcttat tccgtcagcg aaaaatcagc tatttattga caacttggca aggaaaaaac
     1861 accgcagtca tcgaatctgc acaagcattg gggttagatc tacttgataa accaacggat
     1921 caaactatcc gacttgcttc cagttatgcc ttaggagaac ttattaaaga tgcactgctt
     1981 caagaagtag aacaaattat tgtcacttta ggaggaagcg ggtgtagcga tggtggtcta
     2041 ggattactcc aatccttggg tgcttcttta gaagggtaca cagaaggaaa tcctttatta
     2101 tctactaaaa aacttcattt tgatcgaaga aacgttcttg tttctaaagt aaaattgatt
     2161 atcgctgcag atgttaccaa tccatataca ggtcaacaag gagctgcttg gcaatttgga
     2221 agacaaaaag gaggaataga tacgacgcta gctttcttag ataaacaagc aggaaagatt
     2281 gctcagcaaa taaaagattt ctgggaggtg gatttagatg atattccagg aagtggagca
     2341 gcaggcggta ttggtggagc cctttttttg ttgggagcga agatggtttc tggtttcgat
     2401 ttagtcagcc aaatcactgg attggaaaaa aagatcgaac aagcagatgt catcattaca
     2461 ggagaagggc ggatcgatca gcaaacgatc cacggaaaag ttccatacgg tgtagcagtg
     2521 cttgcaaaaa aatatcgaaa accaataatc gctatttgcg gaagtagaga ccaagagcta
     2581 gaaggacttt ctgattttct cccaattgtc ttaagtattc atttgggtcc aataggttta
     2641 gaagaagcaa tggaacacag gcaaacatta gaaaaaacaa aaatcttagg acagacactt
     2701 agcagatttt tcaccatcct cccgaagtag ttgacaaaat cattttcttt atgctatcgt
     2761 aatttcaaat ataggacaaa gtgagggatc cgtgcattga aaaattaagt cgattgatga
     2821 aagctgcatt cagactgtac cttgtggaga caaggaagca gtgtgcagat tttcgggatt
     2881 ggcttggttt tttcgatgta tgggtctttt tgtgtgtatc caacccttgc atcctcttta
     2941 gccagttctg aaaataggac tggtcttttt ttacgaggtg atggaaaatg tctaaaatcg
     3001 aaataaaaaa tctgacattc ggctacgaca gccaaggcac attattattt gaacaagcaa
     3061 atctaaattt tgacacacaa tggaaactag gacttatcgg acgaaacggt cgaggaaaga
     3121 caactttact gaatattcta caaaacaaac taccttatca agggcaagta atccatcagc
     3181 aagaatttgc ctatttcccg caacagacaa aagataaaga acgtttaacc tattacgtgt
     3241 taaatgatat tacggatttt gagatatggg aaatcgaaag agagctccaa ttgatgcaaa
     3301 cagatcctga aatcttatgg agagaattca gcacactatc ggggggagag aagacaaaag
     3361 tcctactggc acttttattt gtggatgaca ctcatttccc gttaatcgat gaaccaacga
     3421 atcatttgga tatctctggt agaaaacaag tagcggctta tttgaaaaag aaaaaacaag
     3481 gcttcatcgt ggtcagccat gaccggggat ttatcgatga agtagtggac catgttttag
     3541 caatcgaaaa aagtcaactg gaactttatc aagggaattt ctctatctat gaagaacaga
     3601 aaaaacttcg tgatgaattt gaaatggctc aaaatgaaaa attgaaaaaa gaagtcagta
     3661 ggctaaagaa aacagcagct gaaaaagccg aatggtctcg ttcccgagaa ggagataaaa
     3721 caaagaaaca agtcggattc atcgatactg aatctagacg agtgaataaa ggagcagtgg
     3781 gtgctgatgc tgcacggacg atgaaacgat ccaaagcaat cgtgaatcgg atggagaccc
     3841 agatcagcga gaaagaaaaa ctattaaaag atatcgaata tatcgattct ttgacgatga
     3901 atagccaagc gtctcaccat aagcgacttt taagcgtaga agatcttcaa ttagggtatg
     3961 aaaatctgtt attcgagcca attcatttta caatcgagcc tcatcagcgg gtggcgattt
     4021 caggtcctaa cggtgcagga aagtcatcca ttatccatta tcttctgggg gcattcaacg
     4081 gcaaggttat aggagaaaaa taccagccaa aacatctgag cattagttat gcaagccaaa
     4141 attatgaaga caatcgagga acgttggcgg aatttgcaga gaaaaaccaa gtagactacc
     4201 aagcattttt gaacaacctc cgaaagcttg ggatggaaag agatgttttt cataacaaga
     4261 tcgagcagat gagtatgggc caacggaaaa aagtggaatt ggctaaatct ttatcacagc
     4321 cagctgaact atatacatgg gatgaaccat tgaattattt ggatgtcttc aatcaagaac
     4381 aattagaaca actgatcttg aacgtgaaac ctgccatgtt actagtggaa catgatcaaa
     4441 ccttcctgga taaagtatct actgagatta tttctcttga gagaatctaa cccttgtaat
     4501 ctcaagaaca acaaggtatg gttaagggga actttgccaa cgaaggagag aatcacatgc
     4561 tttatgtaac gagacacgga gaaacaactt ggaacgcaca aggattagtt tgcggacggg
     4621 cagatgtgcc gctgactgaa aaaggacaga tgcaggcaca gaaactagca gaaaaggtag
     4681 tagatttgcc tgttccaatc acgaaaatca tccattcccc tcttcaacga gcaagagata
     4741 cagcacaagc agttgctgat cgtctcagct tgccgctgac cgttgatgaa cgattggtag
     4801 aaatggattt tggggattat gatggactgc caagcaaaga tgagaacttt caaaaagcac
     4861 gtttagcgtt cgctgtacga ttcccaaatg gggagtcagt cttagatgtg tatgcacgga
     4921 tcgttccttt attgaaagaa tgcatcgaag acgaagaaaa tgtctatctg ttagtttgcc
     4981 ataatgcctt gataagagtg atcaatgctt attttcatcc gatgcctaat gaggggttct
     5041 ttacattcat ggtagataat acagaattgg tttcttatga gtaattatgt ttttgtgtta
     5101 tttaaataaa atatttcaag gcactgcttt tgcttagcag tgcctttttt aggttatgac
     5161 aattcttttg ataatataaa ctcaaccttt agaaatgaaa tttaattatt ttaataaaag
     5221 tattgaaatt ttgtttcttt agatatatac tctagtcata gaaatgaaga cggatacaga
     5281 aaaagaggga taatatgttt ggcttttcaa tatttatgaa cgattctttg tcagaagaga
     5341 agaaacagta tatccgccaa atggcatcca atggctttgt cggtattttc acttccatgc
     5401 atataccaga agatgatgtt tcggcatatc aaaagcgatt gatcgaacta ggaagcgaag
     5461 cgaaaaagaa taaactagaa ttgatggtcg atatttcagg agaggcattg gaacgagcgg
     5521 gtttttcatt agaagatcta acgcctttga aagcaattgg tgtaacaggt ttaagaatgg
     5581 attatcatat ttccaatcag cagattgccg actggtcaca tcaattgaaa atcagtttga
     5641 atgctagcac gatcacgcct aaagatatcg atgaattgaa agaagcagaa gctgatttct
     5701 cacagattga agcttggcat aattattatc cacgtcccga aacagggctg gacaaagaat
     5761 ggtatcagaa aaaaaatcaa tggctcaaaa ttcaaggtct ccttgtccaa ggttttgtac
     5821 caggagatac agagttgaga ggcccactat atcaaggatt gccaactttg gaagaacatc
     5881 gagggatcca tcctcttgca ggcgctctag atctattagc aagtaacacg gatattgttt
     5941 acatcggaga tgcaggtctg tcagaaaacg ttcaggagca atttgcttct ttccaaaaag
     6001 aacagacagt cttgctccat acggaacctg tagatgaaga attttacgaa tatatattag
     6061 ggaagcatac gaatcggcaa gacgatgcaa gagatgtcat acgaagcgta gatgcacgtt
     6121 ttcgagagat tccgcctatc ccagcaagaa atacagctac gcgtatgaaa gggtctatta
     6181 ctttagataa cgaaaagtat ttgcgctata tgggtgagat ccaattaacc aaatatgatt
     6241 tgccagcgga tgaaaaagtg aatgtagtag ctaaagttat caaagaagag ctaccattga
     6301 tcaaccaaat caaagctggt atgaactatc aatttataag aaaagaagga agataaaaat
     6361 gagcaaaatc aatttagaga acctaacaac ggaacgcaga aaccaagaaa cttttggatt
     6421 agatgagatg agtatttctc aagccgttca actaatgaac aaagaagatc agaaagtggc
     6481 cgctgcggtt gaaaaagaac tgacaagtat cgaaccagtg attgctaaca cgattgagtc
     6541 ctttaaaaaa ggtggacgtc tgatttatat gggagccgga acaagtggaa gactaggtgt
     6601 gttagatgct gctgaatgcg taccgacatt cggtgtagaa ccggaaatgg tcataggtct
     6661 aattgctggt ggagaagaag caatgactgt agctgtcgaa ggggccgaag attcttatga
     6721 gctagggcaa gaagatctaa tcaagctgaa tttgacaaaa aatgatatag ttattggtat
     6781 cgcagctagc ggtcgtacac catatgtgat cggtggatta gattacgcaa aatcgatcgg
     6841 tgctgtcaca ggaacgattt cttgcaataa acaagccgaa atcagtaaac acgcggattt
     6901 cccaatcgaa gtcgatgcag gtccagaatt tttgacaggc tctactcgat tgaagtcagg
     6961 aacagcacaa aaacttatct tgaatatgat ttctactatc agtatgattg gtataggtaa
     7021 agtattcaat aacttgatgg tcgatgtcaa accaacaaat gaaaaactaa tcgaacgctc
     7081 aaaacgaatc atcatgcaag cgacagaagc tgattacgaa acagctgaaa agtactttga
     7141 agaagcagat caaaatgtta agctggctat cgtcatgatt ttgacaaata gtgataaaga
     7201 ggaagctcaa gcaaaattag ctgaagcaaa cggatttgta aaagggacct tatgaggggg
     7261 aaaagaccat ggcaaaaaac gaactgactt tggaagaact ggcaaaacaa atttataaag
     7321 gggcaggcgg catgggcaat gtagaaagca ttgtccactg catgactcgt gtgcgtatga
     7381 agatcatcaa tgatgatctt gtaaatgtct ctgaattaaa agcgattcca ggagtgcttg
     7441 gtgtagtaga agatgaacag cttcaagtca tcatcggacc tgggaaagtg aacaaagtag
     7501 cacagcaaat ggttgatttg gcaggcgtga aattaggaga aaaactttct tctgtaagta
     7561 cagctacagc aaattcatca gcatctggaa aagaccgtgt aaacgaacgg gcacaagaaa
     7621 tgaaagcggc acaaaaatcg aaacaaaaac catctaaaat caaatcggta ttaaaagaca
     7681 tttctaatat ctttgtgcca atgattcctg catttgtcgg tactgggatc gtggcaggga
     7741 ttgccgcagt tttagcaaac ttagtcacag ccggaacact tgatgcagca acatggcaac
     7801 aatatatcga tgtgatgaac atcttgaaaa acgggatgtt tagctaccta gttatctata
     7861 caggtatcaa tgctgcacaa gtttttggtg caacaccaac tcttggtgga gttatcgggg
     7921 ccgttgtgat gctgacaggg atgaatccgg aagcaccact gaaaaactta tttactggtg
     7981 aagctcttgc agctggacaa ggtgggatcc taggtgtcat cttcgctgtt tggctattgt
     8041 caatcgttga gaaaaaactg cataaagtag tacctgatgc ggtagatatc atcgttacac
     8101 caactctttc attgattgcc atcggtctga ttgaaatttt cttcatcatg cctttagcag
     8161 gtttcatttc tgatggcatg gtcggtggga tcaactgggt acttggcgtt ggtggagcat
     8221 ttgccggctt cgttctaggc acattattct tgccgatggt tatgttcgga ctgcatcaaa
     8281 ttttgactcc aatccatgtg caaatgattg atgaaactgg tcgtacgcta ttgcttccaa
     8341 ttcttgcgat ggctggtgca ggacaagtgg gtgcagcatt ggcattatgg attcgctgca
     8401 aaaaagataa agaattaaca gaaatgatca aaggagcttt accagtcggg atcctaggaa
     8461 tcggcgaacc attgatttac ggagtaacat tacctctagg acgcccattt attactgctt
     8521 gtatcggtgg aggtatcggc ggggcagtga tcggaatgat cggaaatgtc ggggctatcg
     8581 ctattggacc ttcaggagcc gctttgatcc cattgatctc tgacggtaaa tggttaggct
     8641 acatccttgg attattggca gcttatgctg gtggcttcgt tgctacattc ttcttcggta
     8701 ttcctaaaga ccgtttagaa aaagaagaat tagcgactga aacagccaca gtaaatgaag
     8761 tgcctgaaaa agtagtacct caaacttcag ctgaaagtgt gactatcctc tcatcagttg
     8821 ctgacggaac agttgtttct ttagataaag caagtgatcc agtttttgca caaaagatga
     8881 tgggtgaagg ctactttgta gaaccagaaa acggacatat ctattctcca gttactgggg
     8941 tagtcagctc tgtcttccca acgaaacacg ctattgggat cacaacgcca aatggtttag
     9001 aaatcttgct tcatatggga atcaacacag ttgacttagg cggtacgcca tttgatgtga
     9061 aagtaacaga agggcagcaa gtgacacctg atacgcttgt agcagaagct gacttagaag
     9121 cgatcaaagc agcaggcaaa gaaacatcca tgatcgtctt ggtaaccaat atggatcgtg
     9181 taaaaaactt tgtcttggaa aaaacaggta aagcaaaagc aaaagcacct gtgatggatg
     9241 tcaaagctta atatgataga aacaatagaa acgaaagaag aaactgaa
//
LOCUS       DAJSAW010000237         5873 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.guided.100, whole genome shotgun sequence.
ACCESSION   DAJSAW010000237 DAJSAW010000000
VERSION     DAJSAW010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.guided.100"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(41..1027)
                     /locus_tag="ONT64_003041"
                     /pseudo
     CDS             complement(41..1027)
                     /locus_tag="ONT64_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1179..1874
                     /gene="vanR"
                     /locus_tag="ONT64_003042"
     CDS             1179..1874
                     /gene="vanR"
                     /locus_tag="ONT64_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HCR2304353.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1852..3006
                     /locus_tag="ONT64_003043"
     CDS             1852..3006
                     /locus_tag="ONT64_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCR2304354.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3221..4189
                     /locus_tag="ONT64_003044"
     CDS             3221..4189
                     /locus_tag="ONT64_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HCR2304355.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4182..5213
                     /gene="vanA"
                     /locus_tag="ONT64_003045"
     CDS             4182..5213
                     /gene="vanA"
                     /locus_tag="ONT64_003045"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HCR2304356.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5219..5827
                     /locus_tag="ONT64_003046"
     CDS             5219..5827
                     /locus_tag="ONT64_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HCR2304357.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1772 a         1144 c         1320 g         1637 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccct atttccgata accgtgtatt
       61 gaaaggcatc ttcgaagcca ttatccaata tttcgcaggc ttttgtatat ttaggctggt
      121 cgatatattc accgactaag gcattcttag ctgttcgagc gagttcaata tccgtaaact
      181 taaagatcgc ttttactgct tctctaaacg gttttgaatt cttttttgga atggaactga
      241 agatgtttct taaaaaatgg acctggcatc tctgccaact tgcgttggta aatgacttac
      301 gaatcgcaga cactaggcct ttatgggcat cagaaatgat gagttctgtc ccctttaggc
      361 cgcgttcttt taagtattca aagaagatgg accatgtgtc atcactttct tcattttgaa
      421 tcatgaagcc aatgatttca cggtcgccac cttctgttat cccgatcgca atatggcagc
      481 ttttagaaag cactcgatgg tcctcacgga cttttatgta gagaacatca gtcatcagat
      541 aaggataatt cgtacctgag agtgaacggt tctgccattc gttgaccatc gggtctaact
      601 gctcagtcag gctagaaaca aaagatttcg atacagattt tccacatagc tcttcaacaa
      661 tctttgaaac tttacgtgtc gaaacgcccg aaacatacat ctcaagcatt gaagcgagca
      721 gtgccttttc atttcgctga taacgctcaa acaccgtcgg tgaaaattca ccatcacgtg
      781 ttctaggcac ttttaattcg agtgtaccca cacgagtcgt aaagtctcgc tcataatagc
      841 catttctttg actctgacga ctttctgaac gttcatagtc atcggcttga atatattctg
      901 ttcgttgatt ttccatcaat tggttgaaaa cagtggttaa aatattttta gaaacatcat
      961 cttttaccga atgttcaata atactttgaa cctcttcgtt gttcagtgta aaatgtactt
     1021 gggtcatgta acgtcctcct gggtatgttt ttgtggttaa aaacattgta ccgtaaaagg
     1081 gctgttacat ggccttttat cttttacaca attatatgga ctttatccta taatctgata
     1141 aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg
     1201 tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata
     1261 cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg
     1321 accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa
     1381 taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata
     1441 aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg
     1501 agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg
     1561 agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt
     1621 gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc
     1681 tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg
     1741 acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa
     1801 aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata
     1861 aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc
     1921 gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg
     1981 aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac
     2041 gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg
     2101 attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt
     2161 gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt
     2221 tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag
     2281 cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg
     2341 cacgatatta aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct
     2401 ccagacatgc cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat
     2461 cgactcgaac agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata
     2521 acgctaacaa aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt
     2581 tatcctcagc tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc
     2641 gtgtccggcg accctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct
     2701 gcatacagtg aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg
     2761 tcaatcgaat tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa
     2821 aagttctata ggctggacaa tgctcgttct tccgatacgg gtggcgcggg acttggattg
     2881 gcgattgcaa aagaaattat tgttcagcat ggagggcaga tttacgcgga aagcaatgat
     2941 aactatacga cgtttagggt agagcttcca gcgatgccag acttggttga taaaaggagg
     3001 tcctaagaga tgtatataat tttttaggaa aatctcaagg ttatctttac tttttcttag
     3061 gaaattaaca atttaatatt aagaaacggc tcgttcttac acggtagact taataccgta
     3121 agaacgagcc gttttcgttc ttcagagaaa gatttgacaa gattaccatt ggcatccccg
     3181 ttttatttgg tgcctttcac agaaagggtt ggtcttaatt atgaataaca tcggcattac
     3241 tgtttatgga tgtgagcagg atgaggcaga tgcattccat gctctttcgc ctcgctttgg
     3301 cgttatggca acgataatta acgccaacgt gtcggaatcc aacgccaaat ccgcgccttt
     3361 caatcaatgt atcagtgtgg gacataaatc agagatttcc gcctctattc ttcttgcgct
     3421 gaagagagcc ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc atatagatac
     3481 aactgctgct aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc cggatagcgt
     3541 tgccgattat actatgatgc taattcttat ggcagtacgc aacgtaaaat cgattgtgcg
     3601 ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac tcagcgacat
     3661 gacagttggt gtggtgggaa cgggccagat aggcaaagcg gttattgagc ggctgcgagg
     3721 atttggatgt aaagtgttgg cttatagtcg cagccgaagt atagaggtaa actatgtacc
     3781 gtttgatgag ttgctgcaaa atagcgatat cgttacgctt catgtgccgc tcaatacgga
     3841 tacgcactat attatcagcc acgaacaaat acagagaatg aagcaaggag catttcttat
     3901 caatactggg cgcggtccac ttgtagatac ctatgagttg gttaaagcat tagaaaacgg
     3961 gaaactgggc ggtgccgcat tggatgtatt ggaaggagag gaagagtttt tctactctga
     4021 ttgcacccaa aaaccaattg ataatcaatt tttacttaaa cttcaaagaa tgcctaacgt
     4081 gataatcaca ccgcatacgg cctattatac cgagcaagcg ttgcgtgata ccgttgaaaa
     4141 aaccattaaa aactgtttgg attttgaaag gagacaggag catgaataga ataaaagttg
     4201 caatactgtt tgggggttgc tcagaggagc atgacgtatc ggtaaaatct gcaatagaga
     4261 tagccgctaa cattaataaa gaaaaatacg agccgttata cattggaatt acgaaatctg
     4321 gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat tgctattcag
     4381 ctgtactctc gccggataaa aaaatgcacg gattacttgt taaaaagaac catgaatatg
     4441 aaatcaacca tgttgatgta gcattttcag ctttgcatgg caagtcaggt gaagatggat
     4501 ccatacaagg tctgtttgaa ttgtccggta tcccttttgt aggctgcgat attcaaagct
     4561 cagcaatttg tatggacaaa tcgttgacat acatcgttgc gaaaaatgct gggatagcta
     4621 ctcccgcctt ttgggttatt aataaagatg ataggccggt ggcagctacg tttacctatc
     4681 ctgtttttgt taagccggcg cgttcaggct catccttcgg tgtgaaaaaa gtcaatagcg
     4741 cggacgaatt ggactacgca attgaatcgg caagacaata tgacagcaaa atcttaattg
     4801 agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt gccgcgttag
     4861 ttgttggcga ggtggaccaa atcaggctgc agtacggaat ctttcgtatt catcaggaag
     4921 tcgagccgga aaaaggctct gaaaacgcag ttataaccgt tcccgcagac ctttcagcag
     4981 aggagcgagg acggatacag gaaacggcaa aaaaaatata taaagcgctc ggctgtagag
     5041 gtctagcccg tgtggatatg tttttacaag ataacggccg cattgtactg aacgaagtca
     5101 atactctgcc cggtttcacg tcatacagtc gttatccccg tatgatggcc gctgcaggta
     5161 ttgcacttcc cgaactgatt gaccgcttga tcgtattagc gttaaagggg tgataagcat
     5221 ggaaatagga tttacttttt tagatgaaat agtacacggt gttcgttggg acgctaaata
     5281 tgccacttgg gataatttca ccggaaaacc ggttgacggt tatgaagtaa atcgcattgt
     5341 agggacatac gagttggctg aatcgctttt gaaggcaaaa gaactggctg ctacccaagg
     5401 gtacggattg cttctatggg acggttaccg tcctaagcgt gctgtaaact gttttatgca
     5461 atgggctgca cagccggaaa ataacctgac aaaggaaagt tattatccca atattgaccg
     5521 aactgagatg atttcaaaag gatacgtggc ttcaaaatca agccatagcc gcggcagtgc
     5581 cattgatctt acgctttatc gattagacac gggtgagctt gtaccaatgg ggagccgatt
     5641 tgattttatg gatgaacgct ctcatcatgc ggcaaatgga atatcatgca atgaagcgca
     5701 aaatcgcaga cgtttgcgct ccatcatgga aaacagtggg tttgaagcat atagcctcga
     5761 atggtggcac tatgtattaa gagacgaacc ataccccaat agctattttg atttccccgt
     5821 taaataaact tttaacggtt ctgttgcaaa gttttaaatc tactatcaaa taa
//
LOCUS       DAJSAW010000238         4898 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.guided.109, whole genome shotgun sequence.
ACCESSION   DAJSAW010000238 DAJSAW010000000
VERSION     DAJSAW010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.guided.109"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            302..538
                     /locus_tag="ONT64_003047"
     CDS             302..538
                     /locus_tag="ONT64_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304358.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            545..2497
                     /locus_tag="ONT64_003048"
     CDS             545..2497
                     /locus_tag="ONT64_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCR2304359.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2569..3066)
                     /gene="dfrG"
                     /locus_tag="ONT64_003049"
     CDS             complement(2569..3066)
                     /gene="dfrG"
                     /locus_tag="ONT64_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCR2304360.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3428..4468
                     /locus_tag="ONT64_003050"
     CDS             3428..4468
                     /locus_tag="ONT64_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR2304361.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVAQLP
                     LGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQ
                     LKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSR
                     IDKVRYMFNKIT"
     gene            4587..4754
                     /locus_tag="ONT64_003051"
     CDS             4587..4754
                     /locus_tag="ONT64_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2304362.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(4814..>4898)
                     /locus_tag="ONT64_003052"
     CDS             complement(4814..>4898)
                     /locus_tag="ONT64_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2304363.1"
                     /translation="MDSFTDKELSLMESLASGINEARNIED"
BASE COUNT         1707 a          786 c          887 g         1518 t
ORIGIN      
        1 gtctgaactt tgcggaaatg ttttcaaaat tgaatataca aaaaaaagac aacgtcttgc
       61 atattaagta gttcagtttt ggagtacaaa aagtgaaaat gtaaacctaa atgagtatca
      121 aattgcaact gaatttgata ctcgtttttt attgttaaat caacgtttgt tgttaattta
      181 caccacaaaa tgtaagttaa aatgtatata aaaatgcgaa cgtgaaataa ttattgtagg
      241 aatgcaaatt ggtgtgttaa aatggttaca atttactaag caaaaagggg tgcattttcc
      301 tatgcaattt cttgagccaa aaaataagaa cgctaagtct gtggattggg aaatatcaga
      361 gcaagtaagg gtgatagtta agcaatacgc tgaatatgca gagcgtactg aaagcgaagc
      421 agtggatgaa ttcttattga acattttaga cgataagaag tttattgaat ggattgccaa
      481 caagcgaagt aataagcgga ttgttgaaaa aatgggtatc aaagatagag tgggttgata
      541 gacaatgacc aagcttaaaa gattagccac gaaggaagat gaaattgtag atgttaaaat
      601 tccgatttca aacgaagaat taaaagatag ggctaaacaa tatgatttat taactccaaa
      661 acgatttgca acaaggtata acaaaatgtt gttcttgcca acgtctttta aatggaacgg
      721 ttctgaatat cccattcaat ataattactg catcaatccg ttttgttgta attttgggaa
      781 agaacagcac aaattcaaag atgtaaaagg gaaaccaagt cgatataaaa tgactggtag
      841 ttccaaagat aaaggtcata aaggtatgta ctgcaatgac aatcctattg gcagaggtgt
      901 atcgcaaaac tgtacagtaa cccctttatc taattggtct gttgttgaag aaattaaacg
      961 ccttatagaa atcaatagca tccaagatgt tgagccagat tatcaatttc ataaagaagg
     1021 ctgctcggaa gaagaatcaa ccccattcaa tgagccgaag caattttaca aaagggggaa
     1081 aagtaggggc aaatctcaac gctatcaatg taaggcgtgt aagaagttca ctaatgttct
     1141 accaaaaaga gaagaaacca ctacttatca ccaacaaaag aacaccattc ttccaatgtt
     1201 cgctaagatg gttgttggaa gggtttcagt cagtcgtact tgtgatattt taggaattgg
     1261 agttggaacc tactatcata agctggagtg gctgtatagg cgttgtttgg agtttttaga
     1321 acgatatgag acacaacccc ttcaaacaaa gaaatttaac gagatgtggc ttaatacgga
     1381 caagatgcac tactacctaa ataacgtccg taagaaagga caaggttcaa agaaatatac
     1441 ggggtttgaa gaccttaata tgcaaacgta tatagttgta tctgctgagg tcttatcaag
     1501 atatgtgttc cgttctgatg ttgcctatga ttggaatatt tcaatggatg aattaaatga
     1561 agatacaaga aaattcaaag aagaccatct caatactttt agtcgtaaaa atgataggtt
     1621 agattggtct tattatccac aagaaccatc cgctaacgat agcgaaaata ggaatgctta
     1681 tcttcacgaa ttagggaaaa ttactaatag gagtagattt gtcgatggtt taaatgttga
     1741 tgctccttat actacaactg ctcactattg gttaattaaa caaatggtta atgccgacga
     1801 atggcgaatg attagtgatg atgacttctc tattagaaac gccttttata gagtattcac
     1861 gaaagaatta aggttgagcg atgcacatca ttttatttgc caagttaaca agacgaaaag
     1921 cagaaaacaa tgcttaaaag aattcggaca agcaaaagca gaactgctcg attggggaga
     1981 tatcagagga ttcaaaacaa agtttctacg gactttggca tctcattatc taactgaatt
     2041 actaacaagt catcaattcc acgaagaggc tattagtaaa gatggagaaa gatatcgcaa
     2101 atatgccgat aaccctatca aacatccatt agccactaag gataaaggat tttattcagt
     2161 tgattgtaga acggatttat ctgctctgga accaaacgaa atcgcaaaga tgctattaaa
     2221 tgttaatgac cattcgacca atagctttat tcaacagata agacgttata tatcatctct
     2281 tgaaagacca ttaacaacag ctcgtggtga taagaagagc tatatttacg ctaacttcaa
     2341 tccgaagtat gctcaatttg cgattacaat tctaagaact tactacaact tttgtcgccc
     2401 ttttaaatca gcggataaaa aggttttgac acctgctcaa agattaggga ttacagataa
     2461 acaatttgat tggaaagata taatttattt caaatgacaa aaagagcttg gagtaatccc
     2521 aagctcttgt gctttatctt tactgttctt cttcaattaa cgcacccgtt aactcaataa
     2581 gttttttctt tcatatacat gaaaatagta gttatacgga tttttatcat tctttatccc
     2641 tttttgggca aatacctcat tccattcctc ataattcact tctggaaaaa aagtatctcc
     2701 ttcgaattca tgatgtattt ttgtgatgta cattttctca acataaggga aaaacaaatt
     2761 ataaatctgt tctcctccga aaataaaaat ttcttcttcg tttttacata actcaaaaac
     2821 atcttctatt gaatgaacaa tttcacaacc attaaaggta aaccccttat ctctcgtcag
     2881 aataatattt cttctgtcag gtaaggctct tccgattgat tcaaggttct tcctacctaa
     2941 tattatcgga tgtccctttg tagtattttt aacatattcc cagtccttgg gaatcctcca
     3001 aggaatgtca ttctctttgc caatcactct attcttatcc atcgcagcaa tcaaagaaac
     3061 tttcatttat aacacagcct ttttaactaa tttaccatct aatttcttat ttctatcaaa
     3121 aaaccttttc tcattatcct gcccgttagt tgaataagca attttttcta taagttatat
     3181 tatcataata ccacaaaatc acaaattttc attcaattat cattttcaat tgcattttat
     3241 aaatcatttt gtatgtaatt tggtatgtaa gttaaaatga aaaaagtagt ccattttgaa
     3301 gtacattttg tcattttgta ctccaaaact gaactactta cttgcatata tacgccttta
     3361 tagtggagta ctacatttac gagattcggt tagagtatca gaaaaagaaa aaataaaagt
     3421 tacagaaatg tatacttcaa taaatggtga attatgtaag attgatagag cttattctgg
     3481 agaaattgtt attttgcaaa atgagttttt gaagttaaat agtgttcttg gagatacaaa
     3541 actattgcca cagagaaaaa agattgaaaa tccgcaccct ctactacaaa caactgttga
     3601 accgagtaaa cctgaacaga gagaaatgtt gcttgatgcc cttttggaaa tctcagatag
     3661 tgatccgctt ctacgatatt acgtggattc tacgacacat gaaattatac tttctttctt
     3721 agggaaagta caaatggaag tgattagtgc actgttgcaa gaaaagtatc atgtggagat
     3781 agaaataaaa gagcctacag tcatttatat ggaaagaccg ttaaaaaaag cagagtatac
     3841 cattcacatc gaagttccac cgaatccttt ctgggcttcc attggtctat ctgtagcaca
     3901 gcttccatta gggagcggag tacagtatga gagctcggtt tctcttggat acttaaatca
     3961 atcgtttcaa aatgcagtta tggaggggat acgctatggc tgtgaacaag gattgtatgg
     4021 ttggaatgtg acggactgta aaatctgttt taagtatggc ttatactata gccctgttag
     4081 taccccagca gattttcgga tgcttgctcc tattgtattg gaacaagtct taaaaaaagc
     4141 tggaacagaa ttgttagagc catatcttag ttttaaaatt tatgcgccac aggaatatct
     4201 ttcacgagca tacaacgatg ctcctaaata ttgtgcgaac atcgtagaca ctcaattgaa
     4261 aaataatgag gtcattctta gtggagaaat ccctgctcgg tgtattcaag aatatcgtag
     4321 tgatttaact ttctttacaa atggacgtag tgtttgttta acagagttaa aagggtacca
     4381 tgttactacc ggtgaacctg tttgccagcc ccgtcgtcca aatagtcgga tagataaagt
     4441 acgatatatg ttcaataaaa taacttagtg tattttatgt tgttatataa atatggtttc
     4501 ttgttaaata agatgaaata ttttttaata aagatttgaa ttaaagtgta aaggaggaga
     4561 tagttattat aaactacaag tggatattgt gtcctgtatg tggaaataaa acacgattaa
     4621 agataaggga agatactgaa ttaaaaaaat tccccctcta ttgtccgaaa tgcagacaag
     4681 aaaatttaat tgaaataaag cagttcaaag taactgtgat tacagagcca gacgcaaaga
     4741 cgcagagccg ataaaatgag attaatacaa tctcatttta tcggctcttt ccgttatgta
     4801 tggattcttt taattagtct tcgatgtttc ttgcttcgtt gataccgctg gctaaagatt
     4861 ccattaagga tagttctttg tctgtaaagc tatccatg
//
LOCUS       DAJSAW010000239         4896 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.guided.110, whole genome shotgun sequence.
ACCESSION   DAJSAW010000239 DAJSAW010000000
VERSION     DAJSAW010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.guided.110"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            302..538
                     /locus_tag="ONT64_003053"
     CDS             302..538
                     /locus_tag="ONT64_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2304364.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            545..2497
                     /locus_tag="ONT64_003054"
     CDS             545..2497
                     /locus_tag="ONT64_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCR2304365.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2569..3066)
                     /gene="dfrG"
                     /locus_tag="ONT64_003055"
     CDS             complement(2569..3066)
                     /gene="dfrG"
                     /locus_tag="ONT64_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCR2304366.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3428..4468
                     /locus_tag="ONT64_003056"
     CDS             3428..4468
                     /locus_tag="ONT64_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR2304367.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            4566..4754
                     /locus_tag="ONT64_003057"
     CDS             4566..4754
                     /locus_tag="ONT64_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2304368.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(4812..>4896)
                     /locus_tag="ONT64_003058"
     CDS             complement(4812..>4896)
                     /locus_tag="ONT64_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2304369.1"
                     /translation="MDSFTDKELSLMEALAKGINEARNIEG"
BASE COUNT         1704 a          788 c          873 g         1531 t
ORIGIN      
        1 gtctgaactt tgcggaaatg ttttcaaaat tgaatataca aaaaaaagac aacgtcttgc
       61 atattaagta gttcagtttt ggagtacaaa aagtgaaaat gtaaacctaa atgagtatca
      121 aattgcaact gaatttgata ctcgtttttt attgttaaat caacgtttgt tgttaattta
      181 caccacaaaa tgtaagttaa aatgtatata aaaatgcgaa cgtgaaataa ttattgtagg
      241 aatgcaaatt ggtgtgttaa aatggttaca atttactaag caaaaagggg tgcattttcc
      301 tatgcaattt cttgagccaa aaaataagaa cgctaagtct gtggattggg aaatatcaga
      361 gcaagtaagg gtgatagtta agcaatacgc tgaatatgca gagcgtactg aaagcgaagc
      421 agtggatgaa ttcttattga acattttaga cgataagaag tttattgaat ggattgccaa
      481 caagcgaagt aataagcgga ttgttgaaaa aatgggtatc aaagatagag tgggttgata
      541 gacaatgacc aagcttaaaa gattagccac gaaggaagat gaaattgtag atgttaaaat
      601 tccgatttca aacgaagaat taaaagatag ggctaaacaa tatgatttat taactccaaa
      661 acgatttgca acaaggtata acaaaatgtt gttcttgcca acgtctttta aatggaacgg
      721 ttctgaatat cccattcaat ataattactg catcaatccg ttttgttgta attttgggaa
      781 agaacagcac aaattcaaag atgtaaaagg gaaaccaagt cgatataaaa tgactggtag
      841 ttccaaagat aaaggtcata aaggtatgta ctgcaatgac aatcctattg gcagaggtgt
      901 atcgcaaaac tgtacagtaa cccctttatc taattggtct gttgttgaag aaattaaacg
      961 ccttatagaa atcaatagca tccaagatgt tgagccagat tatcaatttc ataaagaagg
     1021 ctgctcggaa gaagaatcaa ccccattcaa tgagccgaag caattttaca aaagggggaa
     1081 aagtaggggc aaatctcaac gctatcaatg taaggcgtgt aagaagttca ctaatgttct
     1141 accaaaaaga gaagaaacca ctacttatca ccaacaaaag aacaccattc ttccaatgtt
     1201 cgctaagatg gttgttggaa gggtttcagt cagtcgtact tgtgatattt taggaattgg
     1261 agttggaacc tactatcata agctggagtg gctgtatagg cgttgtttgg agtttttaga
     1321 acgatatgag acacaacccc ttcaaacaaa gaaatttaac gagatgtggc ttaatacgga
     1381 caagatgcac tactacctaa ataacgtccg taagaaagga caaggttcaa agaaatatac
     1441 ggggtttgaa gaccttaata tgcaaacgta tatagttgta tctgctgagg tcttatcaag
     1501 atatgtgttc cgttctgatg ttgcctatga ttggaatatt tcaatggatg aattaaatga
     1561 agatacaaga aaattcaaag aagaccatct caatactttt agtcgtaaaa atgataggtt
     1621 agattggtct tattatccac aagaaccatc cgctaacgat agcgaaaata ggaatgctta
     1681 tcttcacgaa ttagggaaaa ttactaatag gagtagattt gtcgatggtt taaatgttga
     1741 tgctccttat actacaactg ctcactattg gttaattaaa caaatggtta atgccgacga
     1801 atggcgaatg attagtgatg atgacttctc tattagaaac gccttttata gagtattcac
     1861 gaaagaatta aggttgagcg atgcacatca ttttatttgc caagttaaca agacgaaaag
     1921 cagaaaacaa tgcttaaaag aattcggaca agcaaaagca gaactgctcg attggggaga
     1981 tatcagagga ttcaaaacaa agtttctacg gactttggca tctcattatc taactgaatt
     2041 actaacaagt catcaattcc acgaagaggc tattagtaaa gatggagaaa gatatcgcaa
     2101 atatgccgat aaccctatca aacatccatt agccactaag gataaaggat tttattcagt
     2161 tgattgtaga acggatttat ctgctctgga accaaacgaa atcgcaaaga tgctattaaa
     2221 tgttaatgac cattcgacca atagctttat tcaacagata agacgttata tatcatctct
     2281 tgaaagacca ttaacaacag ctcgtggtga taagaagagc tatatttacg ctaacttcaa
     2341 tccgaagtat gctcaatttg cgattacaat tctaagaact tactacaact tttgtcgccc
     2401 ttttaaatca gcggataaaa aggttttgac acctgctcaa agattaggga ttacagataa
     2461 acaatttgat tggaaagata taatttattt caaatgacaa aaagagcttg gagtaatccc
     2521 aagctcttgt gctttatctt tactgttctt cttcaattaa cgcacccgtt aactcaataa
     2581 gttttttctt tcatatacat gaaaatagta gttatacgga tttttatcat tctttatccc
     2641 tttttgggca aatacctcat tccattcctc ataattcact tctggaaaaa aagtatctcc
     2701 ttcgaattca tgatgtattt ttgtgatgta cattttctca acataaggga aaaacaaatt
     2761 ataaatctgt tctcctccga aaataaaaat ttcttcttcg tttttacata actcaaaaac
     2821 atcttctatt gaatgaacaa tttcacaacc attaaaggta aaccccttat ctctcgtcag
     2881 aataatattt cttctgtcag gtaaggctct tccgattgat tcaaggttct tcctacctaa
     2941 tattatcgga tgtccctttg tagtattttt aacatattcc cagtccttgg gaatcctcca
     3001 aggaatgtca ttctctttgc caatcactct attcttatcc atcgcagcaa tcaaagaaac
     3061 tttcatttat aacacagcct ttttaactaa tttaccatct aatttcttat ttctatcaaa
     3121 aaaccttttc tcattatcct gcccgttagt tgaataagca attttttcta taagttatat
     3181 tatcataata ccacaaaatc acaaattttc attcaattat cattttcaat tgcattttat
     3241 aaatcatttt gtatgtaatt tggtatgtaa gttaaaatga aaaaagtagt ccattttgaa
     3301 gtacattttg tcattttgta ctccaaaact gaactactta cttgcatata tacgccttta
     3361 tagtggagta ctacatttac gagattcggt tagagtatca gaaaaagaaa aaataaaagt
     3421 tacagaaatg tatacttcaa taaatggtga attatgtaag attgatagag cttattctgg
     3481 agaaattgtt attttgcaaa atgagttttt gaagttaaat agtgttcttg gagatacaaa
     3541 actattgcca cagagaaaaa agattgaaaa tccgcaccct ctactacaaa caactgttga
     3601 accgagtaaa cctgaacaga gagaaatgtt gcttgatgcc cttttggaaa tctcagatag
     3661 tgatccgctt ctacgatatt acgtggattc tacgacacat gaaattatac tttctttctt
     3721 agggaaagta caaatggaag tgattagtgc actgttgcaa gaaaagtatc atgtggagat
     3781 agaactaaaa gagcctacag tcatttatat ggagagaccg ttaaaaaatg cagaatatac
     3841 cattcacatc gaagtgccgc caaatccttt ctgggcttcc attggtttat ctgtatcacc
     3901 gcttccgttg ggaagtggaa tgcagtatga gagctcggtt tctcttggat acttaaatca
     3961 atcatttcaa aatgcagtta tggaagggat acgctatggt tgcgaacaag gattatatgg
     4021 ttggaatgtg acggattgta aaatctgttt taagtacggt ttatactata gccctgttag
     4081 tactccagca gattttcgga tgcttactcc tattgtactg gagcaagcct ttagaaaagc
     4141 tggaacagaa ttgttagagc catatcttag ttttaaagtt tatgcaccac aggaatatct
     4201 ttcacgggca tataacgatg ctcccaaata ttgtgcaaat atcgtaaata ctcaactgaa
     4261 aaataatgag gtcattatta ttggagaaat tcctgctcga tgtattcaag attatcgcaa
     4321 tgatttaact ttttttacaa atgggcttag tgtttgttta gcagagctaa aaggatatca
     4381 ggttaccact ggcgaacctg tttgccagac ccgtcgtcta aatagtcgga tagataaagt
     4441 aagatatatg ttcaataaaa taacttagtg cgttttatgt tgttatataa atatggtttc
     4501 ttattaaata agatgaaata ttctttaata tagatttgaa ttaaagtgga aaggaggaga
     4561 ttgttattat aaactacaag tggatattgt gtcctatttg tggaaataaa acaagactac
     4621 gaatacgagt ggatactata cttaaaaatt tccctttata ctgccccaaa tgtaagaacg
     4681 aaactttaat taatgttcaa aaaatgaata taataacaat caaagagcca gacgccaaga
     4741 cgcagagccg ataatttgag aaatgaaact ctcatcttat cggctctttt tgtttatctg
     4801 aattttactg actagccttc aatatttctt gcttcattga taccttttgc tagtgcttcc
     4861 attaaggata gttctttgtc tgtaaagcta tccatg
//
LOCUS       DAJSAW010000240         4129 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.guided.130, whole genome shotgun sequence.
ACCESSION   DAJSAW010000240 DAJSAW010000000
VERSION     DAJSAW010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.guided.130"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            25..1917
                     /locus_tag="ONT64_003059"
     CDS             25..1917
                     /locus_tag="ONT64_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2304370.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            1924..2301
                     /locus_tag="ONT64_003060"
     CDS             1924..2301
                     /locus_tag="ONT64_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2304371.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            2455..3237
                     /locus_tag="ONT64_003061"
     CDS             2455..3237
                     /locus_tag="ONT64_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HCR2304372.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(3363..4094)
                     /gene="erm(A)"
                     /locus_tag="ONT64_003062"
     CDS             complement(3363..4094)
                     /gene="erm(A)"
                     /locus_tag="ONT64_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HCR2304373.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
BASE COUNT         1482 a          589 c          792 g         1266 t
ORIGIN      
        1 cctcgagaga aaggagcata agaaatgaat gcttctagta aaaggaaaat tattagtcag
       61 agtgagatta gcaaaaaaat agctgtaatg aatgaagaaa tgcaggggtt ttgggctaat
      121 aatagttggg atataagaaa atgtccacat ccttctgcca tagaattaag taagaatcct
      181 gctttaagga atcgttgggt tcgttttgaa cgtgttaaaa atctgtggtt aagaacagaa
      241 ttgaaatatt tttattttta ccatttaaac aatggaatat ggaatgcaaa aactgtctgg
      301 attagaaaag gaacagtaat taataaaatg ttagatttct tagatttaaa gtatcctagc
      361 attacttcaa ttactgaagt tcctattgaa aaagcaatga cggagtatag aacttatttg
      421 acaaaacggg gtgttagaat taccactact aattataaga ttactgctaa tcaagaaaaa
      481 acacctgtaa aagctaattc ctactatgtt actaatctaa aacaatttat ggagttttat
      541 gagaactttt attttgatgg agaggagtgg gataaagacg tttgggatag acgtaactta
      601 cctttgccag atgataaggt taacccaaca caatatgaat atacaattaa ctttaaaggg
      661 tttcggaata catattttaa acaacttgta aaaagatatt gtaagttgag attgaacatg
      721 gatagctttt cctatgtaag tgatattgcc caaagactta aagagttctt taattttctg
      781 gacatgaaat ttaaacaagt tcagagagta caccaattaa cgagagtgga aattgaagca
      841 tatttaagtg aactaaacat gatgggaata aaacctagta caataactgg gaggatctct
      901 atattggaag gactatttag tacccttctt aggctagaat gggatgatgt tccttccaaa
      961 atattaattt attctgagga ctatccgaaa ataccaagag caaaaccacg ctttatagat
     1021 gaattcgtcc tagagcaatt gaacagtcat cttgataaat tacccgaata tatagctacg
     1081 atgactatga ttgttcaaga atgtggaatg aggataagtg aattgtgcac cttgaaaaaa
     1141 ggctgtctat tagaggacaa agatggagat ttctttttaa agtattatca atggaaaatg
     1201 aaaaaggagc atatagttcc aatatctaaa gaggtagctt tacttattaa agttcgggaa
     1261 gataaagttt cagaggaatt tccagatagt gaatacctct ttccaagaaa agatggatcg
     1321 ccattaaaac aagaaacatt tagaggtgag ttaaataaat tagcttatga gcaaaatata
     1381 gtggataaat caggtgagat ttatagattc catgcccatg cctttcgcca tacagtagga
     1441 acaagaatga ttaacaacgg gatgccccag catattgtgc agaaattttt ggggcatgaa
     1501 agcccagaaa tgacaagcag atacgctcat atctttgatg aaactctaaa aaatgaattt
     1561 actaaatttc aggaaaaact ggttaccaat aatggagatg tgcttgatct agatgaagat
     1621 aatgaagtcg atgatgtaga gcttcaatgg ttcaagaaaa atataaatgc acaagtgctt
     1681 ccaaatggtt attgtagatt gccagtagta gcaggtggtt gtccacatgc gaatgcatgc
     1741 ttagattgca ctcacttctg taccagtaag caattcttac cacagcacga agaacagtta
     1801 gagcgtacag aagagttatt agccatagct aaggataaac aatggcaaag acaagtagag
     1861 actaatagcc gtgttaaaga gcgtttagaa caaatcattg gaagtttgac ggggtaatta
     1921 tcaatggata aacaagttag aaatacaaca gaaattgtac gtttggcgaa gcagaaatca
     1981 aaaaagacaa gggaaaaagt agacaaagcg atttctaaat tttcgattga aggtaaagtt
     2041 attaatttta attcaatagc aaaggaagct aatgtttcta aatcatggct ttataaggaa
     2101 cacgatatta ggcaaagaat cgaatccctt cgtgagcgtc aaataacagc aaatgtagtc
     2161 tcaaaaccca agaaaagttc tcgttcggag gaaatcctta ttaaaacctt aaaaagaaga
     2221 gtaatggaat tagaaaaaga aaataaaaaa ttacagaacc aaattcaaaa attatatgga
     2281 gatctgtata ataaagaata attattaatc tgtagacaaa ttgtgaaagg atgtacttaa
     2341 acgctaacgg tcagctttat tgaacagtca tttaggtata tgtccaatct agggtaagta
     2401 aattgagtat caatataaac tttatatgaa cataatcagt tacgaggtga aatcatgagc
     2461 aatttgatta acggaaaaat accaaatcaa gcgattcaaa cattaaaaat cgtaaaagat
     2521 ttatttggaa gttcaatagt tggagtatat ctatttggtt cagcagtaaa tggtggttta
     2581 cgcattaaca gcgatgtaga tgttctagtc gtcgtgaatc atagtttacc tcaattaact
     2641 cgaaaaaaac taacagaaag actaatgact atatcaggaa agattggaaa tacggattct
     2701 gttagaccac ttgaagttac ggttataaat aggagtgaag ttgtcccttg gcaatatcct
     2761 ccaaaaagag aatttatata cggtgagtgg ctcaggggtg aatttgagaa tggacaaatt
     2821 caggaaccaa gctatgatcc tgatttggct attgttttag cacaagcaag aaagaatagt
     2881 atttctctat ttggtcctga ttcttcaagt atacttgtct ccgtaccttt gacagatatt
     2941 cgaagagcaa ttaaggattc tttgccagaa ctaattgagg ggataaaagg tgatgagcgt
     3001 aatgtaattt taaccctagc tcgaatgtgg caaacagtga ctactggtga aattacctcg
     3061 aaagatgtcg ctgcagaatg ggctatacct cttttaccta aagagcatgt aactttactg
     3121 gatatagcta gaaaaggcta tcggggagag tgtgatgata agtgggaagg actatattca
     3181 aaggtgaaag cactcgttaa gtatatgaaa aattctatag aaacttctct caattaggct
     3241 aattttattg caataacagg tgcttacttt taaaactact gatttattga taaatattga
     3301 acaatttttg ggaagaataa agcgtcctct tgtgaaatta gagaacgctt tattacttta
     3361 atttagtgaa acaatttgta actattgaaa atagaaagaa attgttcctt cgatagttta
     3421 ttaatattag tgacatttgc atgcttcaaa gcctgtcgga attggttttt agtgaaaaga
     3481 acacgatatt cacggtttac ccacttataa acaaaagatc gatacttttt gtagtccttc
     3541 tttgaaatca atggttgatg tcgttcaaga acaatcaata cagagtctac acttggctta
     3601 ggatgaaaat atagtggtgg tacttttttg agcattttta tatccatctc caccattaat
     3661 agtaaaccca aagctcgttg cagattttgc aatcttttcg caaatccctt ctcaacgata
     3721 agatagctat atttagcctg actttcaaag gtaattcttt tgacaatatc cgtactgatg
     3781 ttataaggaa tattaccata tatcttatag tttatatgtt ttgggaagga aaattttaga
     3841 atatccgttt gaatcacttt tatattctca gaggggttta ccgcttcttt agtcacttga
     3901 cataagcctc catcaatttc tatagcagta actgatcgac tcattttgac tagctctttg
     3961 gtaaaatgtc cttttcctga tccgatttct attacgttgt cttgtttact gatattcgtg
     4021 tgattcaata tttcttttac atgcttttta gaagtaataa aattttgcgt gtctttaggg
     4081 tttttctggt tcattataac cttctcctta ctggttataa tgaactgac
//
LOCUS       DAJSAW010000241         4061 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.guided.132, whole genome shotgun sequence.
ACCESSION   DAJSAW010000241 DAJSAW010000000
VERSION     DAJSAW010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.guided.132"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            11..1489
                     /gene="msr(C)"
                     /locus_tag="ONT64_003063"
     CDS             11..1489
                     /gene="msr(C)"
                     /locus_tag="ONT64_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCR2304374.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1536..1982
                     /locus_tag="ONT64_003064"
     CDS             1536..1982
                     /locus_tag="ONT64_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCR2304375.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2181..2696)
                     /locus_tag="ONT64_003065"
     CDS             complement(2181..2696)
                     /locus_tag="ONT64_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR2304376.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            2813..4015
                     /locus_tag="ONT64_003066"
     CDS             2813..4015
                     /locus_tag="ONT64_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2304377.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1210 a          696 c          884 g         1271 t
ORIGIN      
        1 tgtaaaacat atggaaaatt tagcagtaaa tataacaaat ctacaagtca gctttggcaa
       61 ccagctagaa ctatctattg attctcttcg tgtctatcag caagatcgta tagggatcat
      121 aggagaaaat ggtgtcggta aatcgacttt gctcaaacta atagccggtg aactttttcc
      181 cgatcatggg aaaatccaaa cagagatcac cttcaactac ctgcctcaat taacctatct
      241 cgctgaagca aaggacctaa atttggaatt agccagtcat ttccagttaa gactggaaga
      301 aacttcagag cggaaatgga gcggagggga agaacgaaag atcgagttga tacgtcttct
      361 ttcttcttat gaacaaggga tgcttctaga cgagccaaca acccatctag atagaaaaag
      421 tattgatcga ctgattgaag agcttcgtta ttattatggt acgctggttt ttgttagtca
      481 tgatcgctat tttctagatg agctagcatc gaaaatctgg gaagtaaaag acggagaaat
      541 ccgagagttt tcggggaatt atagtgccta tctcactcaa aaggaattgg agaaaaagac
      601 tcagctacga gaagcagagt cgatcatgaa agagaaaaaa cgattggaaa aatcgatcca
      661 agaaaagaaa aaacaagcgg aaaagttgga aaaagtgtcc agtaaaaaga aaaagcaaca
      721 aatcagactg gatcggttgt cttcctctaa acaaaaagac agtgtacaaa aagccatcca
      781 aaagaatgcg aaaacattag agagaagact ccaaaaaata ggagaaacaa ccaagccgca
      841 gcagatgaaa caaatccgtt ttccagtacc aaaatctctt gaactccaca gccgctatcc
      901 catcatggga caaaatgtcc aattggaaag aagtgggaga acattactgg taaatggtga
      961 ttttcagttt tctttaggta aaaaaatcgc gattgtcggt gaaaatggtt caggtaagac
     1021 aaccttatta gaacatatcc gcaaacaagg agaaggaatc cttctctctc cgaaagtaag
     1081 ctttcaagta tatcagcaaa agggttatca aatgacatct gaagaatcca tcattcgttt
     1141 tgtcatgaga caaacagagt tttcagaatc gcttgtccgt agtttgctga atcacttagg
     1201 gtttgctcag gaaactttga cgaaaccgtt atgtacatta agtgggggag aagcgacccg
     1261 tctgacgatt gctttgcttt ttactaagcc aagtaatgtg ttgctgttag atgaaccgac
     1321 taattttatt gatatggcaa cgatcgaagc tttagagaag ctaatgcaaa tatatccggg
     1381 aacgattttg tttacttctc atgattctta ctttgtcgag cgtacggctg atgaagttta
     1441 tgaaataaaa gggcagaaaa taaaaaaagt acttacgaga aatttttaaa tgtgctacga
     1501 tgattttcgt gacaaattta aaggagtggt acagattgcc tacaaataaa aaagaaggaa
     1561 taatttttac aacaatgatg tgttttttga tggtcttggg gatgagcgcg tataatttat
     1621 ggctgcatca agatcttcat ttatcaagtt tgttgattgg tctagtgcct ggattcgtgg
     1681 tggctttcat tttggatgtc ttcatcatcg gtgtgctggc gaaaaaaatt gcctttaaac
     1741 taccagtaaa tccagaaagc aaggtccaaa tgatcttaac tatttcatgc ttgatggttt
     1801 taggaatggt gacatgtatg tctttattcg gtgtgatcat ggaacaagga atacctgatc
     1861 aattattctc tgcttatatg aatgcttgga aaatgaatgt gattgccgct ttaccgttgc
     1921 agttgatcat cgttggcccg ctttcaagaa gtgtgctaaa agcaattcaa agtaatgaat
     1981 gatggaacac tgttatttga aaaaaggcct tggcatgctt tttgtcatag tctcttgaat
     2041 gcagttctag aaaaatagtg atccgaacga taggttttgg cctgtcgttc ggatcgcttt
     2101 ttcttttcca actgtattgc tcttttattg aagataattt gctcttcaat agatatttct
     2161 agaaaggttc tgtttatccc ttactcaaaa aactgctgca tttgttctct tacttgctga
     2221 caccattgga gttcctgctg acaaagcata attctgcgct ggatcacaac ataacgtcca
     2281 aattcttgtt tcttgtctag cgagaggtca ttcaattttt tttgattttt ttgcatgatt
     2341 ttgttcagct gttgttctct agtgaatagc agtccagtag ctgttgtttt atcgagcgtc
     2401 gaaaaagcat agatttttgc tagaaattca tctttttgcg taggagcggg agtttcctct
     2461 ttgatccatt cctctacaag agcacgtcct tcatcagtca agtggtagat ctttttttga
     2521 gagtgctcgt ctttgtccaa aacggtcaca taaccctgct ggtctaactt ggcaagcgtt
     2581 gtatagatct gactgtgatg ggcttcccaa aaaattgtga tcagctgctt caattcatac
     2641 ccagaacgtg gtttggataa aagcacgcat aataatatat aggctaaagt attcataaaa
     2701 atcctacctc tcgtcttatt ttctttatta tatcacagta aagtttccac gttttacatg
     2761 tgcatattga catgtaaaat aaatcatgta tgatgctaat agagagaaag ggatggaaat
     2821 attggagaat caaatagata tacaagaaca aacaaatgaa gaaacaaagg ggaaaagttc
     2881 agcgctagtt gtagcagcta cctgtatgat cgcttttatg ggagtgggtc tagtagaccc
     2941 aatcttaaaa acgattgctg cacagttaaa cgctacgcca gctgaaacga ccctgttatt
     3001 tactagttac atgttagtca ctggtgtggt gatgctattt acaggtttct tatctagtcg
     3061 gatcggtttg aaaaaaacat tgatgacagg attgtttatc atcgttgtat tcgctggtct
     3121 tggcggatta tctgggaata tcggaatgct gattgggtta cgtgccggct ggggtgtcgg
     3181 gaatgcttta tttgtctcta ctgctttagc aacgatcgtc agtattttag ttggtaacac
     3241 agaaaaagca atcatgatgt atgaagcagc acttggctgc ggaatggctg ttggccctct
     3301 agtcggtggg gtcttgggca gtggttcatg gcgttatcca ttttttgggg tagcattttt
     3361 gatgttttgc ggttttatag ccctagcggt acttttacct gaaactgaga aaccaaagag
     3421 aaaagtaggc ctttgggaag gtgtcaaagc gttaagcaat cataaacttc gttcagttgg
     3481 actaatcgca ttgttatata atttcggatt ctttacttta cttgcgtatg cgccattttt
     3541 attgattggc tattctgaat tagaagtcgg ttttgtattt tttggctggg gtgtgcttct
     3601 ggctattgct tcgattttcg ttgcaccggc tcttgagcgg aagtttacga cttatcggac
     3661 aattttagca gcatttgtct tattcattat ttgtttgctg ttattaggag ctggaacttc
     3721 tatgccagtc cttgtaccga tcagcattgt gttagcagga tttttccaag gaattatcaa
     3781 tacactgttg acgacgattg ctatggaaat accaggtctg caacgaaacg ttgcttcgtc
     3841 cagctatagt tttgtccgct ttttcggtgg agctttagca ccatttattg caggtgtgat
     3901 tggtgaacat attggtgaaa gctatgcgtt ttattttgca ggtgccattg ttcttatcag
     3961 tgtatgttgt atcatttatc accgtaatta ttttgctaca aatgaagaag aataagaaat
     4021 cagaggttgt gaaaaagctg tcctgcatca agtcataaga a
//
LOCUS       DAJSAW010000242         2570 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.guided.155, whole genome shotgun sequence.
ACCESSION   DAJSAW010000242 DAJSAW010000000
VERSION     DAJSAW010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.guided.155"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..128
                     /locus_tag="ONT64_003067"
     CDS             <1..128
                     /locus_tag="ONT64_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCR2304378.1"
                     /translation="RFYDVLKKNGVNCVIRKEHGTDIDAACGQLRSKQMKKTVAK"
     gene            complement(321..1424)
                     /locus_tag="ONT64_003068"
     CDS             complement(321..1424)
                     /locus_tag="ONT64_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR2304379.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            1977..2525
                     /gene="aac(6')"
                     /locus_tag="ONT64_003069"
     CDS             1977..2525
                     /gene="aac(6')"
                     /locus_tag="ONT64_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCR2304380.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          833 a          448 c          501 g          788 t
ORIGIN      
        1 tgagatttta tgatgtattg aagaaaaacg gcgtaaactg tgtgatacgt aaagaacatg
       61 gtacagatat tgatgcagct tgcggacaat tgcgaagcaa gcaaatgaaa aaaacagttg
      121 ccaaataatc gtatcctact cgttttttca attaccataa aaggagcagt tttcttaatt
      181 aaggaactgt tcaacttaaa taagagaaaa gattctatag cgaaagctat aaagcttttt
      241 ttgacaagaa aaaccctcat caagcaaaga aatgtcctca atttgatgac agcctgcttg
      301 atgggggttt tagcaattat ttaactgatt ttcttatatc cttgctggct ttcttgtgta
      361 gcatgataag tttctacgtc aagaatggcc tgtttaaatt tttctcgggt gatttcataa
      421 ggcacatgtt taatatcatt tgttgccatg gctttatcta agattgcttc taaatcggtc
      481 gtgacattga gattcatgtc ggttaacgat actggaagtt ggttcttttt cataaacaca
      541 tagattttat caaacaaatc gaactgctga tccatcatca ataaggtcag tacaccataa
      601 ctgacagatg ctccgtgaag gtatccctcc attttttcta gaacagtcgt accgtaatag
      661 aatgagtgtg ccatagcaga attatagtta ttgttaacta aaacagaaac caatccggta
      721 gtaacaatga tatccgaaac gacttgttca attgcatatg agggacgatc gtcggcacta
      781 tctttcaatg cttgcgtacc ataagtcaag agacgatcaa tgcacatttt tccgatttgg
      841 attcctaatc tatccgtgta gcctagatca tcgccacgag cagaaaaagt agattcgact
      901 tctttggata aagcatcgcc aatacctgcc cataagtatt tatcaggtgc ttcggcaata
      961 attgctgtat caatgaaggt gtgtacaggt ggttttgaag gaaagaagag tccgtccatt
     1021 acgccttctt cgttatagat gacacatacc gcagtgacgg gcgaacagtt cgaaccaata
     1081 gtgggaaagg tgaagactgg tttagctagt ttttcaccaa tgacttttac agtatcgcaa
     1141 gcacggccac caccgactgc aaacaggacg tctgcttttt ggacactttc atgctggatc
     1201 aactgttcaa tatttccata agttgaatta cccccgtacc agagactggc gacaacctct
     1261 atttcctgtg tcaaagctac ttttattttg ttttctgcct ttgaaagtgc agtcttccct
     1321 ccaatcagca ctacttttgt cccatattct ttgacgattc catttgcttt ttcatacgca
     1381 tctgttccaa tggtataatt tggtaagaaa acactttgac tcatattcat ttccttcttt
     1441 cttcgttgaa acgggttaaa attttaaaga gtgatcgatc tgactgcggc tgtagatgga
     1501 gtagaggtga acctaagaca ggataagtga tgaataaggt ggcatgaaca ccttgaaatc
     1561 atcaataagt atcctaaaaa cgaacaagta caaaaacatt gaaaacgaca ataatgaatt
     1621 tttgttcctc ttagtatttc taccgattcc ctttgctttt ttgtaattat ctaaaaattc
     1681 taaaaattaa gattattata tgaaaacagg ttttatttgt caattcataa ttggtttttt
     1741 ttaaagcaaa agataatcta tatcatgatg aacaaacaga gttttttcct ccaattgatg
     1801 gctttcctga tctttcctat tttgaataaa gtgtttctgt ataatttttt tattttataa
     1861 gtaagataaa taatgaaaga atagagatac tgattggtag ctaaagattt ttgagcgaga
     1921 acatttcgtt ttttcaaaag gactgttaga ataagcaaga acaaggggga agacgtatga
     1981 taatcagtga atttgaccgt aataatccag tattgaaaga tcagctttct gatttactga
     2041 gactgacttg gccggaagaa tatggagaca gctcggcaga agaagtagaa gaaatgatga
     2101 atccagaacg aatcgcggta gcagcggtag accaagatga gttagtagga tttattggtg
     2161 caatccctca atacggtatc acaggttggg aattgcatcc attagttgta gaaagctccc
     2221 gacgaaagaa ccaaataggt actcgattag tcaattactt agaaaaagaa gtagcttcca
     2281 gaggaggaat cacgatttat ttaggtacgg atgatttaga ccatggaaca acgttaagtc
     2341 aaacggacct gtatgagcat acatttgata aagtggcttc catccagaac cttcgtgaac
     2401 atccgtatga attctatgaa aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa
     2461 atggctggga caaaccggat atttggatgg caaaaacgat tattcctcga ccaaattctc
     2521 aataataaaa agaggttgtg acagaagtaa tcagcttcaa gaaataagaa
//
LOCUS       DAJSAW010000243         2371 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.guided.163, whole genome shotgun sequence.
ACCESSION   DAJSAW010000243 DAJSAW010000000
VERSION     DAJSAW010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.guided.163"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT64_003070"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT64_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCR2304381.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLDSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2060..2227
                     /locus_tag="ONT64_003071"
     CDS             2060..2227
                     /locus_tag="ONT64_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2304382.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2287..>2371)
                     /locus_tag="ONT64_003072"
     CDS             complement(2287..>2371)
                     /locus_tag="ONT64_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2304383.1"
                     /translation="MDSFTDKELSLMESLASGINEARNIED"
BASE COUNT          817 a          361 c          483 g          710 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatac gtctgggaaa
      601 ttattggaag cattagaact cgaacaagag gaaagcataa gatttcataa ttgttccctg
      661 ttccctgttt atcacggaag tgcaaaaaac aatataggga ttgataacct tatagaagtg
      721 attacgaata aattttattc atcaacacat cgaggtcagt ctgaactttg cggaaaagtt
      781 ttcaaaattg agtattcgga aaaaagacag cgtcttgcat atatacgtct ttatagtggc
      841 gtactgcatt tgcgagattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaatcgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagtta gatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaaata
     1261 aaagagccta cagtcattta tatggaaaga ccgttaaaaa aagcagagta taccattcac
     1321 atcgaagttc caccgaatcc tttctgggct tccattggtc tatctgtagc acagcttcca
     1381 ttagggagcg gagtacagta tgagagctcg gtttctcttg gatacttaaa tcaatcgttt
     1441 caaaatgcag ttatggaggg gatacgctat ggctgtgaac aaggattgta tggttggaat
     1501 gtgacggact gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg agatagttat
     2041 tataaactac aagtggatat tgtgtcctgt atgtggaaat aaaacacgat taaagataag
     2101 ggaagatact gaattaaaaa aattccccct ctattgtccg aaatgcagac aagaaaattt
     2161 aattgaaata aagcagttca aagtaactgt gattacagag ccagacgcaa agacgcagag
     2221 ccgataaaat gagattaata caatctcatt ttatcggctc tttccgttat gtatggattc
     2281 ttttaattag tcttcgatgt ttcttgcttc gttgataccg ctggctaaag attccattaa
     2341 ggatagttct ttgtctgtaa agctatccat g
//
LOCUS       DAJSAW010000244         2371 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.guided.164, whole genome shotgun sequence.
ACCESSION   DAJSAW010000244 DAJSAW010000000
VERSION     DAJSAW010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.guided.164"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT64_003073"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT64_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCR2304384.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLDSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2060..2227
                     /locus_tag="ONT64_003074"
     CDS             2060..2227
                     /locus_tag="ONT64_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2304385.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2287..>2371)
                     /locus_tag="ONT64_003075"
     CDS             complement(2287..>2371)
                     /locus_tag="ONT64_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2304386.1"
                     /translation="MDSFTDKELSLMESLASGINEARNIED"
BASE COUNT          816 a          361 c          484 g          710 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatac gtctgggaaa
      601 ttattggaag cattagaact cgaacaagag gaaagcataa gatttcataa ttgttccctg
      661 ttccctgttt atcacggaag tgcaaaaaac aatataggga ttgataacct tatagaagtg
      721 attacgaata aattttattc atcaacacat cgaggtcagt ctgaactttg cggaaaagtt
      781 ttcaaaattg agtattcgga aaaaagacag cgtcttgcat atatacgtct ttatagtggc
      841 gtactgcatt tgcgagattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaatcgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagtta gatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaaata
     1261 aaagagccta cagtcattta tatggaaaga ccgttaaaaa aagcagagta taccattcac
     1321 atcgaagttc caccgaatcc tttctgggct tccattggtc tatctgtagc acagcttcca
     1381 ttagggagcg gagtacagta tgagagctcg gtttctcttg gatacttaaa tcaatcgttt
     1441 caaaatgcag ttatggaggg gatacgctat ggctgtgaac aaggattgta tggttggaat
     1501 gtgacggact gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg agatagttat
     2041 tataaactac aagtggatat tgtgtcctgt atgtggaaat aaaacacgat taaagataag
     2101 ggaagatact gaattaaaaa aattccccct ctattgtccg aaatgcagac aagaaaattt
     2161 aattgaaata aagcagttca aagtaactgt gattacagag ccagacgcaa agacgcagag
     2221 ccgataaaat gagattaata caatctcatt ttatcggctc tttccgttat gtatggattc
     2281 ttttaattag tcttcgatgt ttcttgcttc gttgataccg ctggctaaag attccattaa
     2341 ggatagttct ttgtctgtaa agctatccat g
//
LOCUS       DAJSAW010000245         2369 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.guided.165, whole genome shotgun sequence.
ACCESSION   DAJSAW010000245 DAJSAW010000000
VERSION     DAJSAW010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.guided.165"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT64_003076"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT64_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCR2304387.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLDSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="ONT64_003077"
     CDS             2039..2227
                     /locus_tag="ONT64_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2304388.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..>2369)
                     /locus_tag="ONT64_003078"
     CDS             complement(2285..>2369)
                     /locus_tag="ONT64_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2304389.1"
                     /translation="MDSFTDKELSLMEALAKGINEARNIEG"
BASE COUNT          814 a          363 c          469 g          723 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatac gtctgggaaa
      601 ttattggaag cattagaact cgaacaagag gaaagcataa gatttcataa ttgttccctg
      661 ttccctgttt atcacggaag tgcaaaaaac aatataggga ttgataacct tatagaagtg
      721 attacgaata aattttattc atcaacacat cgaggtcagt ctgaactttg cggaaaagtt
      781 ttcaaaattg agtattcgga aaaaagacag cgtcttgcat atatacgtct ttatagtggc
      841 gtactgcatt tgcgagattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaatcgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagtta gatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatg
//
LOCUS       DAJSAW010000246         2369 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.guided.166, whole genome shotgun sequence.
ACCESSION   DAJSAW010000246 DAJSAW010000000
VERSION     DAJSAW010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.guided.166"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT64_003079"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT64_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCR2304390.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLDSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="ONT64_003080"
     CDS             2039..2227
                     /locus_tag="ONT64_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2304391.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..>2369)
                     /locus_tag="ONT64_003081"
     CDS             complement(2285..>2369)
                     /locus_tag="ONT64_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2304392.1"
                     /translation="MDSFTDKELSLMEALAKGINEARNIEG"
BASE COUNT          813 a          363 c          470 g          723 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatac gtctgggaaa
      601 ttattggaag cattagaact cgaacaagag gaaagcataa gatttcataa ttgttccctg
      661 ttccctgttt atcacggaag tgcaaaaaac aatataggga ttgataacct tatagaagtg
      721 attacgaata aattttattc atcaacacat cgaggtcagt ctgaactttg cggaaaagtt
      781 ttcaaaattg agtattcgga aaaaagacag cgtcttgcat atatacgtct ttatagtggc
      841 gtactgcatt tgcgagattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaatcgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagtta gatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatg
//
LOCUS       DAJSAW010000247         1781 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.guided.175, whole genome shotgun sequence.
ACCESSION   DAJSAW010000247 DAJSAW010000000
VERSION     DAJSAW010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.guided.175"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            24..416
                     /locus_tag="ONT64_003082"
     CDS             24..416
                     /locus_tag="ONT64_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2304393.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="ONT64_003083"
     CDS             417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="ONT64_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCR2304394.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          731 a          136 c          303 g          611 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg agattctgga attatagatg
     1621 aatattgtga ttttatatac ttacttgaag atagtgaaga agaaatagga acaaattttg
     1681 gagaagatat attaagaatg tatggaaata tagatattga gaaagcaaaa gaatatcaag
     1741 atatagttga agaatattat cctattgaaa ctattgttta t
//
LOCUS       DAJSAW010000248         1504 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.guided.182, whole genome shotgun sequence.
ACCESSION   DAJSAW010000248 DAJSAW010000000
VERSION     DAJSAW010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.guided.182"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            262..345
                     /locus_tag="ONT64_003084"
                     /pseudo
     CDS             262..345
                     /locus_tag="ONT64_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="ONT64_003085"
     CDS             394..477
                     /locus_tag="ONT64_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HCR2304395.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="ONT64_003086"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="ONT64_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCR2304396.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAJSAW010000249         1382 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.guided.190, whole genome shotgun sequence.
ACCESSION   DAJSAW010000249 DAJSAW010000000
VERSION     DAJSAW010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.guided.190"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1311
                     /locus_tag="ONT64_003087"
     CDS             <1..1311
                     /locus_tag="ONT64_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HCR2304397.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          584 a          108 c          234 g          456 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacggttct gttgcaaagt tttaaatcta ctatcaaata
     1381 ag
//
LOCUS       DAJSAW010000250         1350 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-11
            SAMN30692154-rid15768423.guided.192, whole genome shotgun sequence.
ACCESSION   DAJSAW010000250 DAJSAW010000000
VERSION     DAJSAW010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692154
            Sequence Read Archive: SRR21444923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472582.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 21 of 73
            Pseudo Genes (incomplete)         :: 42 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 5 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692154-rid15768423.guided.192"
                     /isolate="VRED13-11"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            47..298
                     /locus_tag="ONT64_003088"
                     /pseudo
     CDS             47..298
                     /locus_tag="ONT64_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="ONT64_003089"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="ONT64_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCR2304398.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
